LOCUS       DASSWZ010000001         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_467011, whole genome shotgun sequence.
ACCESSION   DASSWZ010000001 DASSWZ010000000
VERSION     DASSWZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467011"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /gene="trmFO"
                     /locus_tag="VFN58_00005"
     CDS             <1..763
                     /gene="trmFO"
                     /locus_tag="VFN58_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET8916397.1"
                     /translation="IDALLTAKRVPTYSFERFVPFEGCMPIEEMADRGKETLAFGPMR
                     AVGLIEPRSGIRPYAVVQLRQENQEATLYNLVGFQTKMTYPEQRRVFAMIPGLEKAEF
                     VRLGSLHRNTFINSPQHLLPTLQWRQRLDLFFAGQMTGVEGYIESAATGLLAGINAAR
                     LVAEKPPVVPPPTTALGALLRYITDPARKTFQPMNVNFGLLPTLSEPFRKKAKKEMMA
                     QRAVSDMEAWVRNTEGVEAFVPLDAAPNAHSAMAR"
     gene            760..1698
                     /locus_tag="VFN58_00010"
     CDS             760..1698
                     /locus_tag="VFN58_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET8916398.1"
                     /translation="MSSRRFAFIDIGTNTILGLIAELKNDGSFDVLDDLAEITRLGEG
                     VQRTGRISPEGEERSLKVLRCYLERFESLNVEEIIAVGTSALRDARNSAEVRGRFKEQ
                     LGFDVRVISGDEEAAYSFLAVQRGLPLNRRELLVVDVGGGSTEFIRGSEAGVSQAVSI
                     NIGSVRLTEQFLHSDPVQTEECEKMMRAIERELALLPHQWLKDSSILTLVGIAGTFTT
                     LSAVEKKLARYAHGEVHGSHLTLSEVRRQVALYQGRTIAERKAIDGLEPKRADVILAG
                     ATLIERTMTFFSLQQAIVSDHGVRYGLLHECLKEGN"
     gene            1804..2073
                     /locus_tag="VFN58_00015"
     CDS             1804..2073
                     /locus_tag="VFN58_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET8916399.1"
                     /translation="MTKGDLIDAVAKGAKLSKRAAGEAVDATFEGIARAIKRSKRFQV
                     PGFGTFTVRSRKARKGRNPQTGAVINIRASRTVGFKPAPDLKKGL"
     gene            2211..3128
                     /locus_tag="VFN58_00020"
     CDS             2211..3128
                     /locus_tag="VFN58_00020"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET8916400.1"
                     /translation="MSLKTEKIWFDGELVPWEDAKIHVLAHALHYGTAYFEGIRCYAL
                     ADGRSAVFRLEEHIRRLADSGKILGFPLPYTVEQLQQATLEVIRANRLKECYIRPLVF
                     LGLGDMGLYAPNNPINVCIAVWPWGAYLGDEGIKNGIRAKVSSYTRHHVNVMMTKSKA
                     SGNYINSVLAKTEVKKAGYDEAIMLDAEGYVSEASGENIFIVHDRKLKTTPLTSILPG
                     ITRDSILTVARDKGYEVVEERFTRDELYTADEAFFTGTAAELTPIREVDDRQIGAGCR
                     GPVTADIQQTFFDVIKGKDKKYSKWLAYL"
     gene            3220..3561
                     /locus_tag="VFN58_00025"
     CDS             3220..3561
                     /locus_tag="VFN58_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HET8916401.1"
                     /translation="MTMLPCDTNAHGTIFGGVILSHIDLAGAIAASRQAPRNFVTRAM
                     REVGFIAPVYLGDVVSFYTSVVSEGTTSVTVQVIVEAERSKEPRRQVKVTEAEVVYVA
                     VDENGKSTPIS"
     gene            complement(3558..>3758)
                     /locus_tag="VFN58_00030"
     CDS             complement(3558..>3758)
                     /locus_tag="VFN58_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916402.1"
                     /translation="SASLGLKAAYSSSAFCNDREIGEPSLAQLGQPTLPVFDTPPWAI
                     TSVFVNSKRKNIKRRIMSDLSV"
BASE COUNT          884 a          943 c         1080 g          851 t
ORIGIN      
        1 catcgatgcc ctattaaccg ccaaacgagt accgacctac agcttcgaac ggtttgtgcc
       61 gttcgaaggt tgcatgccga tcgaagagat ggcggaccgc ggcaaggaaa cattggcatt
      121 cggtcctatg agggccgtag ggctaattga gccgcgaagc ggcattcggc cctatgctgt
      181 ggttcagttg cggcaggaga atcaagaagc cacgctctat aacctcgtcg gtttccagac
      241 aaagatgacc tatccggagc agcggcgggt ttttgcaatg attccaggac tggaaaaagc
      301 ggagttcgtc cgactcggca gtttacaccg caacactttt atcaattctc cgcagcatct
      361 gctgcccacg ttgcagtggc gccagcgctt ggatttgttc ttcgcgggac aaatgaccgg
      421 cgtcgagggt tacattgagt cggccgcaac gggtctctta gcgggcatca atgccgcgcg
      481 gttagtcgct gaaaagccac ctgttgttcc gccgccaacc acggctctgg gggcgttgct
      541 gcgctatatc acggatccgg cgcgcaagac tttccagccg atgaatgtga actttggttt
      601 gctccccacc ctgtcggaac cttttcgaaa aaaagccaaa aaagaaatga tggcgcaacg
      661 agccgtgtcg gatatggagg cctgggtcag aaacactgaa ggggttgagg cattcgtccc
      721 cttagatgct gcccccaacg cgcactcggc gatggcgcga tgagttcccg gcgctttgcg
      781 ttcatcgata tcggtaccaa cacgatcctg ggcttgatcg ccgagctgaa aaatgacggt
      841 agctttgatg tccttgacga tcttgcggaa atcacgcggc taggagaggg tgttcaacgg
      901 accggccgca ttagcccaga gggagaagag agaagtctca aagttctgcg atgttatctg
      961 gaacgttttg agagtctcaa cgttgaagag atcatagcgg tgggtacaag tgctctaaga
     1021 gatgcccgca acagcgctga agtgcgaggt cgcttcaagg agcagctcgg gtttgatgtt
     1081 cgcgtcatat cgggcgacga ggaggccgcc tattcttttc tagcggttca gcggggactt
     1141 cctctcaacc ggcgggaatt gttggttgtt gacgtcggcg gcggaagtac ggaatttatc
     1201 cgcggcagcg aggcgggcgt ttcccaggcc gttagcatca atatcgggtc ggtgcggctc
     1261 accgagcaat ttctccattc cgatccggta caaaccgaag agtgcgagaa gatgatgcgc
     1321 gccattgaga gagagctggc attattgccg catcaatggc taaaagatag ttctattctg
     1381 accctcgtag gaattgccgg gacctttacg accctttccg ccgtggaaaa aaaattggcg
     1441 cgctatgccc acggtgaagt tcacggcagc cacttgactc tgagcgaggt tcggcggcag
     1501 gtagcacttt atcaaggcag gacaattgca gagcgtaagg cgatcgatgg cttggagccc
     1561 aagcgggccg atgttattct tgccggcgca accttgatcg agaggaccat gacttttttt
     1621 agtttacagc aggcgatcgt gagcgatcac ggcgtgcgct acggattgct tcatgaatgc
     1681 ttaaaagagg gcaattaaac tgttgacatt ttcggctgaa ttcggaatta tctccccttg
     1741 tttttgcagc actggctgca atcaatcaag ttgacaactc gccatgaagg gaggtgaacg
     1801 caaatgacca aaggcgatct aatcgacgca gtggccaaag gtgccaagct tagcaaaaga
     1861 gcagcagggg aggctgtgga cgcaaccttc gagggcatcg cccgcgccat caaaaggagc
     1921 aaacgctttc aggtaccagg attcgggaca ttcacggtgc gctcgcgcaa agcacgcaag
     1981 ggaagaaatc cgcagactgg ggctgtcatc aacattagag ctagtcgaac tgtaggattc
     2041 aaacccgccc ccgatttaaa gaaagggtta tgatgttact ggtaatccgt tgaaggccac
     2101 cacggggaac cgggtggcct ttttcttgtt gcgacccttg atgaggctcc ggcgggcacg
     2161 tacaatgaac ggaatccgta accgttgaga agcctcgaaa gcgagagtcc atgtcactca
     2221 agactgagaa aatttggttt gacggggagt tggttccctg ggaagacgcg aaaatccacg
     2281 ttttggctca tgctcttcat tacggcaccg cttacttcga ggggattcgt tgttatgcct
     2341 tggccgatgg ccgctccgcg gtttttcgcc tggaagagca tatccgtcgc ttggccgact
     2401 ccggtaagat tctggggttc ccgcttcctt acacggttga acaactccag caagccacat
     2461 tggaagtgat tcgggcaaat aggctaaagg aatgttacat ccggcccttg gtttttttgg
     2521 gtctaggcga catgggactt tatgcgccga ataatcccat caacgtttgc atcgcggttt
     2581 ggccatgggg ggcttatctc ggcgatgagg gaatcaagaa tggcatccgt gcgaaggtat
     2641 cctcgtatac tcgccaccac gtgaacgtga tgatgaccaa gagcaaggcg tcggggaatt
     2701 acatcaactc ggtactggcc aaaaccgaag tcaagaaagc gggttacgac gaagcgatta
     2761 tgctcgatgc cgagggttat gtctcggaag ccagcgggga gaacatcttc atcgtccacg
     2821 accggaagct gaaaacaacg ccgttgacat cgatcctgcc cggcatcacc cgggactcca
     2881 tcctgaccgt ggcccgcgac aaaggctacg aagtggtcga agagcgcttt acccgtgacg
     2941 agctttacac ggcggatgaa gcgtttttta ccgggaccgc cgctgagctg acacctattc
     3001 gcgaagtgga tgaccgccag atcggcgccg gctgtcgcgg tcctgtcacg gccgatattc
     3061 aacagacttt cttcgacgtt atcaaaggca aagataaaaa gtattcgaag tggttggctt
     3121 atctctgacg attttcttga cgagacgtta gctcctgttc gattcgttta tcatctagat
     3181 gcccagctgg tggctgaatt ccgagaacca acgttacgga tgaccatgct gccgtgcgat
     3241 accaatgcac acggaactat cttcggcggg gtaatcttga gtcacatcga cttggccggc
     3301 gcgattgcgg cctctcggca agcgccgagg aactttgtca cccgagcgat gagagaagtt
     3361 ggttttatag ccccggtcta tctcggggac gtagtgagct tctatacttc tgtggtctct
     3421 gaaggaacga cgtccgtgac cgtccaagtg atcgtcgaag ccgagagaag taaagaaccg
     3481 cggcggcaag tcaaagttac ggaagccgag gtcgtatacg tcgcggtgga tgaaaatggc
     3541 aaatcgactc cgattagcta aaccgatagg tcactcatga ttcttctttt gatgttcttt
     3601 ctcttgctgt taacaaacac ggaggttatc gcccaaggtg gcgtatcgaa aactggcaga
     3661 gtgggttgcc ctaattgcgc taatgatggc tcaccgattt cccggtcatt gcaaaaggcc
     3721 gacgagctgt atgctgcctt taaacccaag gaggcgct
//
LOCUS       DASSWZ010000002         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_42473, whole genome shotgun sequence.
ACCESSION   DASSWZ010000002 DASSWZ010000000
VERSION     DASSWZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42473"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFN58_00035"
     CDS             <1..748
                     /locus_tag="VFN58_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8916403.1"
                     /translation="PQSYGWTGSYASMGALIAHHYREQTGEGQQVDVSAQACLLWAFS
                     HANTFWDLNRHVEKRAGSFITGRSITGAKMRVFWPCQDGYLNFIIYGGEAGRRTNQAL
                     VEWMDSKGMAPDFLKQKDWKTFDIAQVGQEEIDRMEEPIENFFQGVTKAEFFKQVVKR
                     QMLGYPVASVKEIFADPQHEARSFWQTIEHPELQTSIDYPGGFAKFSEGACRIWRRAP
                     LIGEHNREIYGQELGMSPAQIAELQKQGVI"
     gene            754..1968
                     /locus_tag="VFN58_00040"
     CDS             754..1968
                     /locus_tag="VFN58_00040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8916404.1"
                     /translation="MQSLKGVKVVEFAAFAAGPAVGKHLADHGATVVHIESRARLDGF
                     RAHYPPYKDNIHGLNRSGLFALCNNDKLDITLNLKKAPQATELAKRIVAWSDVVIENF
                     SPGTMARLGLDYQSLKQIKPDLVMLSSSNLGQTGPHAHHAGFGSQLSSLAGFTHLTGY
                     PDGSPQILYGPYIDYIAVAYGAVAILAALDYKRRTGKGNYIDTAQYETGLQYLAPILL
                     DYKVNGKVASRNANRDPSASPHGTYPCKGDDHWCVLSVYSESEWTALCDAMGNPGWTG
                     DTKFSSHESRKANEDDLDRRIGEWTIGFTAQEVMEKLQSAGVRAGVVNTMKEVYSDPQ
                     LAQRPQWVELEHPEIGTMHYQRPPFLLTKTPPGPSRRDPLLAENNEFFYKDLLGLSQQ
                     EYNQLVEAEVIY"
     gene            2400..3098
                     /locus_tag="VFN58_00045"
     CDS             2400..3098
                     /locus_tag="VFN58_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET8916405.1"
                     /translation="MEKRHKTTRARTLTLTIVCTKSALVGVLFIPFLLTMDYRPQKEP
                     MAESPILVPVPVVQKPPPRELMRIYSIVRSHRPDITEAEAWEISEVILEESSGYGLDP
                     MLVLAVIDVESKFQYGAVSPAGARGIMQILPYVAQSLVQKIGLHQLSHSNSFRPEFLD
                     DPVLNIKLGVYYLHDLKKSFRNLTHALTAYNMGPTETKNRLENDLEIPEEYSTLVLAA
                     YRQYKSGKTKQPIF"
     gene            3130..>3996
                     /locus_tag="VFN58_00050"
     CDS             3130..>3996
                     /locus_tag="VFN58_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HET8916406.1"
                     /translation="MYSNSRILVAGCGAIGSVFGCLLREAGHSVALLGRESHLKAIRT
                     GGLRLEGIWGDHYADGFELATQTTELSGVYDLVLISVKAYHTESMARDICPVVDRDGL
                     AVSLQNGLGNIETLAKIFGAERSLGANVLVGAIIPEAGRVTVTVQAAPVIIGPLEVSD
                     CVMLERIHYWIRAFKRANIPCEHTVRILSYLWAKIFYNAPLNPLGALLQVHYGVLGDA
                     AELRQIMDRLVDEAFLVAKTNGVELLWNTVDDYRALFYRHLVPSTYNHQSSMLQDLQR
                     GRRTEIDAINGAI"
BASE COUNT          946 a         1114 c         1040 g          896 t
ORIGIN      
        1 tccacagtct tatggctgga ccggttccta cgcctccatg ggagcgctga tcgcgcacca
       61 ttatcgagag cagaccggcg aggggcagca ggtcgacgtc tccgcgcagg cttgtttgct
      121 ctgggccttt tcccatgcca atacattttg ggatctcaac cgccacgtgg aaaagcgcgc
      181 gggctcattc attaccgggc gaagcattac cggtgcgaag atgagggttt tctggccctg
      241 ccaagacggc tatctcaact tcatcatcta cggaggcgag gccgggagac gaaccaacca
      301 agcgctggtc gagtggatgg acagcaaagg catggcgccg gattttctga agcagaaaga
      361 ttggaaaact tttgatatcg cccaggtcgg ccaggaagag atcgatcgta tggaagagcc
      421 catcgaaaac tttttccagg gtgtcaccaa agccgagttt tttaaacaag tcgttaaacg
      481 acaaatgctc ggctacccgg tggccagcgt caaagagatt tttgccgatc cgcagcatga
      541 agcacgcagc ttctggcaga cgattgagca tccggaacta caaacttcaa tcgactatcc
      601 tggaggcttc gcgaaattct ccgagggagc ctgccggatc tggcggcgcg ctccattgat
      661 cggcgagcac aaccgggaga tctacggtca ggagcttgga atgtcgccgg cgcaaatcgc
      721 tgagctgcaa aaacagggag ttatttgatc gccatgcagt cgctaaaggg tgtcaaggtc
      781 gttgaatttg ccgctttcgc cgccgggccg gctgtcggca agcacttagc cgatcacggc
      841 gcgacggtgg ttcatatcga atccagagcc cgcctggatg gcttccgcgc ccattatccg
      901 ccttacaaag acaacattca cggcctcaat cgctccggtc tcttcgctct ttgcaacaac
      961 gataaattgg acatcactct gaatctaaag aaagcgccac aagcaacaga gctggctaag
     1021 agaattgttg cctggtcaga tgtggtcata gaaaacttta gcccgggaac catggcgcgg
     1081 ctgggtctcg attaccaaag tctcaagcaa ataaaacccg atctcgtcat gctgagcagc
     1141 tccaatctag gtcagaccgg accgcacgct catcacgcgg gctttggttc ccagctctct
     1201 tcgttggctg gcttcacgca tctgacggga tatcccgacg gctcgccgca gattctctat
     1261 ggaccttaca tcgattacat cgccgtcgcc tacggtgccg tcgcgatcct cgccgccctg
     1321 gattacaagc gacgcaccgg caaaggcaac tacatcgaca cggcccaata cgagacgggg
     1381 ctccagtacc tggcgccgat tctgttggac tacaaagtca acggtaaagt tgccagccgc
     1441 aatgcgaatc gcgatccctc tgcatcaccg cacggcacct atccgtgcaa aggcgatgat
     1501 cactggtgtg tcttgagcgt ctactctgaa tccgaatgga cagcactttg cgatgcaatg
     1561 gggaatccgg ggtggaccgg tgatacaaaa ttttcttccc acgaatcacg caaggccaac
     1621 gaagatgatc tcgatcgcag aatcggcgag tggaccatcg gcttcacggc tcaggaagtg
     1681 atggagaaac ttcaatcagc cggcgtcaga gccggcgtcg tcaacaccat gaaagaggtc
     1741 tacagcgatc ctcagctggc tcagcggccg cagtgggttg aactggaaca tccggagatc
     1801 ggtacgatgc actaccaacg cccccctttc ttactcacaa agacaccgcc cggaccgtcg
     1861 aggcgcgacc cgcttttggc ggaaaacaat gaattcttct ataaggatct gctgggtttg
     1921 agccagcagg aatataacca gctcgtcgaa gcagaagtta tttactgaac cggattccgc
     1981 agttaaaccg aaaatgccgg aagcgaggtg caatcctgga gcagcgaagg cgtccgactg
     2041 tgcaatttca aagtcatttt gcgctctcgc acgtcgttgc tatgaggcta accttccctc
     2101 gaagcaccgc tgatcgatgt ccactcagag gcagaaaccc atcgtcttcg ctgcggaagg
     2161 gttgtgcctc gcgtctctgt cgatcccaac tgcggaatcc gggctgaatt ctgctcgacg
     2221 acaattattc tattccggat tcttcgcggg aataaccatc tcgcattccc cgcgaatttt
     2281 tttcagattt tttcagattt tttcgttgac atcccttccg ctctttgata gctaaaccaa
     2341 agcctttgta gctaccgtag ctcaccacca caactaatag aatagctgag gtctggagta
     2401 tggagaaacg tcataaaaca acgcgcgctc gtactttgac tttgacgatt gtttgcacca
     2461 aatcggctct cgtcggagtt ctatttattc cttttctttt gaccatggac taccggccgc
     2521 agaaagagcc catggccgag tcccccattt tggtcccggt tcccgtggtc caaaagccac
     2581 caccgaggga actgatgagg atctattcca ttgtaagatc acatcggccc gacatcaccg
     2641 aggcagaagc atgggagatc tcagaagtca tactggaaga gagttcgggc tacgggcttg
     2701 acccgatgct ggtgttggcc gtgatcgatg tggaaagtaa atttcagtat ggggcggttt
     2761 cacccgccgg ggccagggga atcatgcaga ttcttcccta tgtggcacaa tccctcgtgc
     2821 agaaaatcgg tctccatcaa ctgtcacatt ccaattcttt tagaccggag ttcttggatg
     2881 atcccgttct caacataaag ctcggcgttt actatctcca tgatctgaaa aaaagttttc
     2941 gcaacctcac tcacgctcta accgcctata acatgggacc caccgaaacc aagaaccgcc
     3001 tggaaaacga cctcgagatc cccgaagaat attcgactct ggtgctggct gcataccggc
     3061 agtacaagag cgggaaaacc aaacaaccca tcttttgacg actcgatata gcgccagcat
     3121 cgccgccgaa tgtacagcaa ctcaagaata cttgttgcag ggtgcggtgc cattggatcg
     3181 gttttcggct gcttactcag agaagcaggg cacagcgtcg ctctccttgg gcgtgagagc
     3241 cacctgaagg ccatccgcac cggcggtctc aggctggagg gcatctgggg cgatcactac
     3301 gcggacggct ttgaactcgc cacccaaaca accgagttat ccggcgtcta tgatctcgtc
     3361 ttaatctcgg tgaaagctta tcacaccgaa tcaatggctc gcgacatttg cccagtagtc
     3421 gaccgtgacg gcctcgcggt ttctttgcag aacggtcttg gcaacatcga aaccttggcc
     3481 aaaatcttcg gcgcagaaag gagtttgggg gcaaatgttc ttgtcggcgc aatcattccg
     3541 gaagccggga gggttacagt caccgttcaa gctgcgcctg ttatcatcgg tcccctcgag
     3601 gtttctgatt gcgtgatgtt ggaacggatt cactactgga tcagagcttt caagcgcgcc
     3661 aatatcccgt gcgagcacac agtgcgaata ctttcttacc tttgggccaa gatattctac
     3721 aatgctcccc tcaatccgct gggagcgttg cttcaggtcc actacggtgt gctcggcgac
     3781 gcagccgaat tgcgccaaat tatggatcgg ctcgtcgacg aggcattcct tgtggccaaa
     3841 accaacggcg tcgaactctt gtggaacacg gtggatgact atcgggcgct tttctatcgt
     3901 cacctggttc cttccacgta caatcaccag agctcgatgt tgcaagattt gcagcgcggt
     3961 cggcgcaccg agatagacgc gattaacggc gcgatc
//
LOCUS       DASSWZ010000003         7010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_325613, whole genome shotgun sequence.
ACCESSION   DASSWZ010000003 DASSWZ010000000
VERSION     DASSWZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325613"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFN58_00055"
     CDS             <1..778
                     /locus_tag="VFN58_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009049075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8916407.1"
                     /translation="VDLPGVGTNLQDRYEVSVVNRMEDDWEALRGAKFHRGDSQYQQW
                     VTEKKGVYITNGGVLVVIKKSKPNRPLPDLFCIGLLGKFEGYFPGYSKSIERHQNYLS
                     WVVLKAHTCNTAGTVTLRSADARDTPLINFHYFDEKCDPDLEDLDSVVEGIKFVRSLT
                     GGMSEYIAEEELPGKNLQSHDELRRFVRENAWGHHASCSCAIGPRGNGGVVDSKFRVY
                     GTKGLRIVDASVFPKIPGFFIVSSVYMIAEKASDVILADV"
     gene            854..2179
                     /locus_tag="VFN58_00060"
     CDS             854..2179
                     /locus_tag="VFN58_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET8916408.1"
                     /translation="MATDAIFQPLAFRNLKVKNRIFRSSISGRFDNYDGSGNLARINW
                     EEKFARGGVGAIISSFVPVHIRGRIMPNYAMIDSDDKIPFWRKVGEKVHEYDCKYIMQ
                     LSHSGRQRDVPGVENFMHKALSSTSNKDTFHGLLCEAATVEQIHELERLFANGARRAR
                     AAGLDGIELHASHGYLFTQFLSSAINDRKDEYGGSLENRARFLLEVIRAIRKEVGNDF
                     HLQVKTNAVDEGSALFPWEKKGNLLEESITVCKWIEESGADAIHVSMGNIFPHPMMPP
                     GGFPIDEASWWYGNMASSGVRGYMNYTLFHFKVLRPIFLYLWNRSKKTRPVEGACADE
                     SRAVKQQVGIPVINTGGYQHGALIRKVISEGYCDGVAIARSLIANNDLPKIIEQGRDL
                     PDRPCTFSNRCLVNAMANPLGCYDLRRFNDDHDAMVKEVMTVFHPPPFP"
     gene            2239..3786
                     /locus_tag="VFN58_00065"
     CDS             2239..3786
                     /locus_tag="VFN58_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8916409.1"
                     /translation="MSEKAVRTKAKDQVLTDRVLETPVDRYQLKFRRKQVIVLVIILL
                     LLWGARLVRRFLVDRPVSYSKIEDHFKYGSIGSEPGGSLFYAVGGLLPPYWIFKALPK
                     ICPDKLPGGYASLGFIFESGHDLPIGVSKRLRLGFEQVGLNCAVCHTGSYRDEPNGGP
                     RIVLGMPAHSLDLQKFFSFVIDCSLDERFNADNVIEKVREAGGRVGVVDRYLYRTQLV
                     PRIRETTRQLKERIQILLSEQVPAWGVGRVDTFNPYKAIQFNWDLKALPQEQLTGASD
                     FPSLWNQKPRDGMQLHWDGNNNSVDERNLSAALGAGVTPVTVDHPRLQRVRNWIWTLP
                     PPQYPYEIDPVLAAKGRDLYVYYCSSCHDFDGSEVGRVEPIAEINTDPRRLNSYTYIF
                     AANQYTLYPDSRYRFVHFRKTNGYANQPLDGIWARAPYLHNGSVPTLRDLLEPPEKRP
                     GEFFRGYDLYDRKKVGFISNVAEANGRRFFNYDTKIAGNGNGGHLYGTKLTPEAKDAL
                     VEYMKTL"
     gene            3824..5671
                     /locus_tag="VFN58_00070"
     CDS             3824..5671
                     /locus_tag="VFN58_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008187941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8916410.1"
                     /translation="MNQYERLAKWYKRVTWFGILVNLLFVFPLVFAPRTILNLLALDV
                     QPVLWARSAGILVFIMCVFYFPASLNLKRYRLYAWLAIFPARFLGAVFFILAVFVVGY
                     PQGYLSIGFVDLGILSLQLLILLNIRRVERGEAPFRKFASMSAIAAVLVAMLGSLVWY
                     NSFRERPQRLADESIEEYFKYGSIGAENQSGLPYWIWLVLPRMFPDHLPGPGGYASFG
                     LPWEQGREMPIGFTKKTIGFDRVAFNCAFCHTAVVRRSADDPVPIIYPGGPSHTFNAL
                     AYERFLFKAASDPRFTTKNIMNAIAEVTQLSILDKGLYRFVLIPFTRKALLKQKDAFA
                     WTDGRPDWGPGRIDPFNPVKVAILQKVNPKVGVGDTIGNADMVAIWNLRARGGMAYHW
                     DGLNTDVTEVVLSSAIGDGATPKSIPLGNLHRLQNWLMNLKPAPFPFLDKVDASLATR
                     GAVIFKQQCASCHAFGGEKTGKVLPASEAGTDPHRAQMWTPDAAQAYHDYSKPYPWAF
                     THFRSTGGYVNVPLDAVWIRAPYLHNGSVPSLVDLLSPPAERPKVFFRGYDVYDPEKV
                     GFVSSGLEAERYGFRYDTSRPGNGNGGHLWGTDLGAQDKKALIEYLKTL"
     gene            5847..6623
                     /locus_tag="VFN58_00075"
     CDS             5847..6623
                     /locus_tag="VFN58_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916411.1"
                     /translation="MIISIINHTNGQLSDEEVQHAIRAINCQIKQDFEPYWSLGATLR
                     LEGKAGAKPKPQEPSDMRGEAVIYLWDNVNVANALGYHDRNNRGIPFGFVFTELSKSL
                     GESWTVTLSHEALELVGDPEVNLLVAGPHPANPKQDVFHWYEMCDAVQAETYKIDDID
                     VSNFVLPLYFTGSEELGGRNDFLGRIHDGKTLRSFNVNPGGYVGFFNPQTGEHETFSR
                     KGDTQAEERMKIKFKAKGARRAIRYRRFEVKTAAAKKTAA"
     gene            complement(6627..>7010)
                     /locus_tag="VFN58_00080"
     CDS             complement(6627..>7010)
                     /locus_tag="VFN58_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase"
                     /protein_id="HET8916412.1"
                     /translation="LAEFARPHGFAIAETQFQVFILNASRRLFSDRFLTSSFRPEFYT
                     HLGVKWVNENGPDGIIKEKGKPNGHEMEVSPLKRVLQRTIPELKPELEPVVNVFDPWA
                     RDRGDYYSLQWKPRTGAGSDVAFKD"
BASE COUNT         1560 a         1914 c         2012 g         1524 t
ORIGIN      
        1 agtggatctc ccgggtgtcg gtacgaatct tcaggatcgc tatgaggtca gcgtagtcaa
       61 tcgcatggag gacgattggg aggcgctgcg gggcgcgaaa ttccataggg gggacagcca
      121 gtatcaacag tgggtgacgg agaagaaagg tgtttacatc accaatggtg gagtgctcgt
      181 ggtcatcaag aagtcgaaac cgaatcggcc gcttcccgac cttttctgta tcggactact
      241 ggggaagttc gagggctatt tccccggcta ctcaaaatct atcgaacggc atcaaaatta
      301 cctgagctgg gtcgtcttga aggctcatac ctgcaacacg gctggaactg tgacactgag
      361 gtccgcggat gctcgcgaca cacccctgat caacttccat tatttcgatg aaaaatgcga
      421 tcccgatctg gaggatcttg actctgtcgt ggagggaatc aagtttgtgc gctcgctgac
      481 cgggggcatg agcgagtaca tcgcggagga agaactaccg ggaaaaaatc ttcagagcca
      541 cgatgagctg cggcggttcg taagggagaa tgcctggggt caccacgcct catgcagttg
      601 tgcgatagga ccgcgaggaa atggcggcgt ggtcgacagc aagtttcgag tttatggcac
      661 gaaggggctg cggatcgtcg atgcctcggt cttccccaag attccgggct tttttatcgt
      721 gtcgtcggtc tacatgattg ctgagaaagc gagcgacgtt attttggcgg acgtttgaag
      781 atgaatcaga ccacgttgaa cggtttgaac tgtttgaaac gattgaagct gattgttggg
      841 aggtgaccta gccatggcga ccgacgcaat ctttcaaccg ctggcttttc ggaaccttaa
      901 ggtcaagaac cggattttcc gctcaagcat ttccggccgg ttcgacaact acgacgggtc
      961 aggaaacctg gcacggatca actgggaaga aaaattcgcg cgcgggggcg tcggcgcgat
     1021 catttcatcc ttcgtgcccg tgcacattcg cgggcgcatc atgcccaact acgccatgat
     1081 tgacagcgac gacaagatcc cgttctggcg caaggtgggc gagaaggtcc atgagtacga
     1141 ctgcaaatac atcatgcagt tgagccattc aggccgccag cgcgatgttc ctggcgtcga
     1201 gaattttatg cataaggcgc tgtcttcgac cagcaacaag gataccttcc acggcctgct
     1261 ctgcgaagcc gccactgtgg agcagatcca cgagctggag cggctcttcg ccaacggcgc
     1321 ccgccgagcc agagctgctg gtctcgacgg catcgagctg cacgcctcac acggctatct
     1381 cttcacgcag tttcttagct cggcgatcaa cgacaggaaa gatgaatacg gcggctcgct
     1441 tgaaaaccgt gcccggtttc ttctcgaagt aatccgggca atccgcaagg aggtcggcaa
     1501 cgacttccat ttacaagtaa agaccaacgc ggtcgatgaa gggagcgccc tctttccctg
     1561 ggagaagaaa ggaaaccttc tggaagaatc catcactgtt tgcaagtgga tcgaggaatc
     1621 cggggcggac gcgattcacg tatcgatggg caacatcttt ccccatccga tgatgcctcc
     1681 gggcggcttc ccgatcgatg aggcgagttg gtggtacggc aacatggcct caagcggcgt
     1741 gcgcggctac atgaattaca ccctctttca cttcaaggtt ctaagaccga tctttcttta
     1801 tctttggaat cgcagcaaga agacgcgccc ggtcgaaggg gcatgcgccg acgaatcacg
     1861 cgctgtgaag caacaggtcg gcattcccgt catcaacacc ggtggctacc agcacggcgc
     1921 tttgatccgc aaggtcatct ccgaaggcta ttgcgacggg gtcgcgatcg ctcggtcgct
     1981 gatcgccaac aacgatctcc caaaaatcat agagcaggga agggacctgc ctgacaggcc
     2041 gtgcacgttc tcgaaccgtt gtctggtgaa cgccatggcc aacccgctgg gttgctatga
     2101 tctcagacgc ttcaacgatg atcacgatgc catggtgaag gaggtcatga cggtctttca
     2161 tccgccgccg tttccctaag atgaagctgc cgcggttgga cagaaaactg attttttgaa
     2221 cttcacagag gtgccgctat gagtgaaaaa gcagttcgca ccaaagcgaa agaccaagtt
     2281 ctgaccgatc gagtcctgga aactccggtc gatcgatacc aacttaaatt ccgccgcaag
     2341 caggtgattg tcttggtgat cattctgctc ctcttatggg gagcacgctt agtccggcgc
     2401 tttctggtgg accggccggt tagttacagc aaaatcgaag atcacttcaa atacggctcg
     2461 atcggcagcg agccgggggg atcgctcttc tacgctgtag gaggtctgct ccctccctac
     2521 tggattttca aagccctgcc gaagatttgc cccgacaagc tacccggtgg ttacgcctcg
     2581 ctcggcttta ttttcgagag cgggcacgac ctgccgattg gagtttcgaa gcgtctgcgg
     2641 cttggcttcg agcaagtcgg gctgaactgc gccgtttgcc ataccggctc ctatcgtgac
     2701 gagcccaacg gcggcccccg catcgtccta gggatgccgg cgcacagtct ggatcttcag
     2761 aaatttttta gctttgttat cgactgcagt ctggatgagc gcttcaatgc ggacaacgtg
     2821 atcgaaaagg tgagagaagc cggaggccgc gtcggcgttg tcgatcgata cctgtaccgc
     2881 actcaactgg ttccccggat ccgcgaaacc actcgccagc taaaggagcg aattcagatt
     2941 ctgttgagcg agcaagtgcc cgcgtgggga gtggggcggg tcgatacgtt caatccctac
     3001 aaggcgattc aattcaactg ggacttaaag gcccttcctc aagagcagct aaccggcgcg
     3061 tccgatttcc cttcgctttg gaaccagaag ccgcgcgacg gcatgcagct tcactgggac
     3121 ggcaataaca actccgtgga cgagcgcaac ttgagcgccg cgttaggcgc tggtgtgacg
     3181 ccggttacgg tcgatcaccc gagactccaa cgagtccgca actggatctg gacgctgccg
     3241 ccgccgcaat atccctatga gatcgatcct gtgttagccg ccaaaggcag agacctgtat
     3301 gtgtactact gctcgagctg ccacgacttc gacggctcgg aggtgggccg cgtcgagccc
     3361 atcgccgaga tcaacaccga cccgcgccgt ctcaattcct atacctatat attcgccgcc
     3421 aaccagtaca cgctttaccc ggactccaga taccggttcg tccactttcg taaaacgaat
     3481 ggctatgcga accaacctct agacggtatc tgggctcgag cgccgtacct gcacaatggc
     3541 tcggtgccga ccttgcgcga ccttctggaa ccgccggaga agcggcctgg tgagttcttt
     3601 cgcggctatg atctctacga ccgtaaaaaa gtcggcttta tctcgaacgt cgccgaagcg
     3661 aacgggcgac gctttttcaa ttacgatacc aaaatcgcgg gcaacggaaa cggcggccat
     3721 ctttacggca cgaagctcac gccggaggca aaggatgccc tggtcgagta catgaagacg
     3781 ttgtgagcat ctgtcgtgac cgggtgaaga aggggagcga ggaatgaacc aatacgagcg
     3841 cctggcgaaa tggtacaaac gggttacctg gttcggcatt ttggtgaacc tgctgtttgt
     3901 gttcccgctg gtgtttgcgc cgcggacgat cctaaatctg ctcgcgctcg atgtgcagcc
     3961 ggtgctctgg gctcgcagcg ctggcatcct cgtgtttatc atgtgcgtct tctatttccc
     4021 cgcgagcctc aacctgaagc gctatcggct ctacgcctgg ctggccattt ttcccgcgcg
     4081 gtttttaggc gccgtgtttt tcatcctggc cgtattcgtt gtcggttatc cgcagggtta
     4141 cctgtcgatc ggctttgtcg atcttggtat tctttccctc caactcctaa tcctattgaa
     4201 cattcggcgc gtcgaacgcg gagaggcgcc gtttcgcaag ttcgcgagca tgagcgcaat
     4261 cgctgcggtg ctcgtcgcca tgttggggag tcttgtgtgg tacaactcat ttcgcgagcg
     4321 gccccagcgg ttggcggacg agtcgataga agaatacttc aaatacggct caatcggggc
     4381 agagaatcaa agcgggctgc cctactggat ctggctcgtt ctaccgcgca tgtttcccga
     4441 tcatctgccg ggtccgggcg gctatgcgtc gtttggcctg ccatgggagc agggacggga
     4501 aatgcccatc ggattcacca agaaaaccat cggattcgac cgggtcgcgt tcaactgcgc
     4561 tttttgccac accgccgtgg tccggcgttc ggccgacgac ccggttccga tcatctatcc
     4621 cggcggtcca tcgcatactt tcaatgcgct ggcttacgaa cgttttcttt tcaaggccgc
     4681 gagtgatccg cgctttacga ccaagaacat catgaacgcg attgccgaag tgacccagct
     4741 ctcgatactc gataaggggc tctatcgttt cgtgctgatt ccgttcacta ggaaggcttt
     4801 gcttaagcag aaggatgctt tcgcctggac ggatggccgg ccggattggg gtcctggacg
     4861 catcgatccg ttcaatccgg tcaaggtggc gattctccaa aaggtcaacc ccaaggtcgg
     4921 cgtcggcgac accatcggca acgccgacat ggttgcgatc tggaacctgc gcgcgcgggg
     4981 cggcatggcg taccactggg acggcttgaa caccgacgtg accgaagtcg tgcttagctc
     5041 ggcgatcgga gacggggcaa cccccaagtc cattccactc ggcaacttgc accgtctgca
     5101 gaattggctg atgaatctca agccagcgcc gtttcctttt ctggataagg tcgatgcgtc
     5161 acttgcgacc cgtggcgcgg tcatttttaa acagcagtgc gcttcctgcc acgcgttcgg
     5221 cggcgagaag accggcaagg tgctgcccgc cagcgaggca ggcaccgatc cgcatcgcgc
     5281 gcagatgtgg acgccggacg cggcgcaagc ctaccacgat tactcgaagc cgtatccatg
     5341 ggcgttcacc cacttccgct cgaccggcgg ttatgtcaac gtgccgctcg acgcggtgtg
     5401 gattcgtgcg ccttatttgc acaacggctc ggtgccgtcg ttggttgatc tgctctcccc
     5461 cccggccgaa cgacccaagg tgttttttcg gggatatgat gtctatgacc cggagaaagt
     5521 cggatttgtg agcagtggtc tggaagcgga gcgttacggc ttccgttacg atacgagccg
     5581 gcccggaaac gggaacggcg gacatctctg gggaacggat ctcggtgcgc aagacaagaa
     5641 ggcactcatt gagtatttga aaacccttta gtgaactgaa tcattaaaga cttcgcaact
     5701 ggaagatttg aaggtgagtt tttgtaagca acgaggagtg ctgatcaaag ggaaatcgta
     5761 cggtaaagta actacaaggc gcattccgga aacaaggatt cattccgacg gatcaagaga
     5821 ctaacaacta accaaggagg acgtctatga taatttctat catcaaccac accaacggcc
     5881 agctcagcga cgaagaggtg cagcacgcga ttcgcgccat caattgtcag atcaagcagg
     5941 actttgaacc ttactggagc ttgggggcga cgctaagact ggaaggaaag gcgggagcaa
     6001 aacccaagcc gcaggaaccg tccgacatgc gcggcgaagc ggtgatctac ctttgggaca
     6061 acgtcaacgt agccaacgct ttgggttacc acgacagaaa taataggggc attcccttcg
     6121 gctttgtgtt tactgaactt tcgaagagct tgggagaaag ttggaccgtg acgttgtctc
     6181 acgaagctct cgaactcgtc ggcgacccag aggtcaacct gctggttgcg ggaccccatc
     6241 ccgccaatcc caagcaggac gtgtttcatt ggtacgagat gtgcgacgca gtgcaggcgg
     6301 aaacctacaa gatagacgac atcgacgttt ctaacttcgt gctaccgttg tacttcaccg
     6361 gcagtgaaga actgggaggg cggaacgatt ttctcggccg cattcatgat ggcaagacgc
     6421 ttcgatcctt caacgtcaac cccggcggtt acgtcggatt tttcaatccg caaacgggag
     6481 agcacgaaac tttttcacgc aaaggcgaca cgcaagccga agaacgtatg aagattaagt
     6541 tcaaagccaa gggcgccagg cgcgctatcc gctatcgccg atttgaagtg aagacggcag
     6601 ctgcgaaaaa aactgccgcg tgatttttaa tccttgaatg ctacatccga gccggcgccc
     6661 gtccgcggct tccattgtaa ggaatagtaa tcaccgcggt cgcgggccca ggggtcgaat
     6721 acattgacga cgggctcgag ttccggtttg agttccggaa tcgtgcgttg aagcacgcgc
     6781 tttaagggcg agacttccat ctcatgaccg ttgggcttgc ctttctcttt gataatgccg
     6841 tcgggtccgt tttcattgac ccacttgaca ccgaggtggg tgtagaattc agggcgaaag
     6901 ctcgaagtaa gaaaacggtc gctaaaaagc cggcgtgagg cattgaggat aaaaacctga
     6961 aattgagttt cggcgatcgc gaagccgtgc ggccgcgcaa attctgccaa
//
LOCUS       DASSWZ010000004         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_226595, whole genome shotgun sequence.
ACCESSION   DASSWZ010000004 DASSWZ010000000
VERSION     DASSWZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226595"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFN58_00085"
     CDS             complement(<1..801)
                     /locus_tag="VFN58_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916413.1"
                     /translation="MNRKLRLVVLGMMGRCPFGGQSWLYLNWLRGLHKLGHEVWYVED
                     DTVWPYDPERNSVTDDCSYAVRHIAGCMKRAGMPDTWAFRPAYRTGECHGISGSELND
                     LYRTCDALLNVVGATDLREEHLAARFRVYVECDPVIAELQLAKSDPHTVVALANHHAI
                     VTYGENFGSPDCGVPLNGQRYGKTRQPVDLDFWPMTYDPEARFFTTIGNYRQDGNDVE
                     YNGEIYHWSKHHEWEKFIDLPRHTSQPLELAMKVEDPADRLRLESYGWK"
     gene            complement(865..2646)
                     /locus_tag="VFN58_00090"
     CDS             complement(865..2646)
                     /locus_tag="VFN58_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916414.1"
                     /translation="MRLRVRSYLRPYLPTFVVALTQVAFISALELLKPWPLKIIIDHV
                     MTETPMPWAFAVSWSSQNLLLFACAGLVVVYLLLGGLRMLNDYTTIRIGQKMVNDLRR
                     DLYSQIQRLSLSFYHRRQVGDLLYRLTSDTYAIQTLTMNGLFPVLCSLTLFVGMFLVM
                     IRMDPLLTFLALIICPILFVFVMISPLRGWLIASATRMHQQKSSVFSLVQWAIPALRV
                     TQAFTKEEEEHRRFMALSQKSLKAELHFYLLQNFYSGTVSVVIAIGTAVVIWVGARHV
                     LAGTLTIGALIVFTAYLTSLYGAIDSIAQAYGSIEGAKVSLQRVFEIMEVEGELEEGS
                     RVFPKGGARGEVAWSEVSFHYSAGQPVLRHVDLNVRPGQKVAIVGRTGAGKSTLVSLL
                     PRFYDPKSGRVTIDGVDIREYQLRSLRRQIAMVLQPPLVFPLTIRENLMYGRPEATEE
                     EVANAARLARIHETIVALPEGYDTVVGEQGATLSEGEKQRLTIARAILINAPILILDE
                     PTSSVDAETEALIMEGLRELTAGRTTFIIAHRLSTVRQADLIIVVRGGKIVEQGTFEE
                     LMRLRGDFAKLYRTQFSAQEEKTRALV"
     gene            complement(2660..>3092)
                     /locus_tag="VFN58_00095"
     CDS             complement(2660..>3092)
                     /locus_tag="VFN58_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET8916415.1"
                     /translation="GREKESLLYSIMDFLLPRKYLIAVDQGWSHWDLEICQGPWAKAQ
                     IRAATENHGSGKRLLRVRCALRMSRMSFTCLSLYAVLMTVAVFLSMPQIAVAALVTGL
                     IHGGAILHQKFRLGRVLYHVLESLAHKLDFVPAQKPSEEAL"
BASE COUNT          728 a          864 c          854 g          646 t
ORIGIN      
        1 tttccagccg tagctttcca gccgcagccg atccgcgggg tcctccactt tcatggcgag
       61 ctccaagggc tggctcgtgt gccgcggcag atcgataaac ttctcccact cgtggtgctt
      121 gctccagtga tagatctcgc cattgtactc cacgtcattg ccgtcctggc ggtagttgcc
      181 gatcgtcgta aaaaagcgcg cttcaggatc gtaggtcatc ggccaaaaat ccaaatccac
      241 cggctgacgt gtcttcccat agcgctgacc gttgagggga accccgcagt caggcgagcc
      301 aaaattttcg ccataggtga cgatcgcgtg atggttcgcg agcgcgacga ccgtgtgagg
      361 atcgcttttg gcgagctgta actcggcaat gaccggatcg cactccacat agacgcgaaa
      421 gcgggcggcc agatgctcct cgcgcaagtc ggtggctccc accacattta gcagcgcgtc
      481 gcacgttcgg tatagatcgt ttagctcgga gccgctaatg ccgtggcatt ctcctgtccg
      541 atacgccggg cgaaacgccc aggtatctgg catgccggcc cgcttcatgc accccgcgat
      601 atggcggaca gcatacgaac agtcgtctgt gacgctgtta cgctccggat cgtatggcca
      661 gaccgtgtca tcctcgacgt accagacctc atggcctagc ttgtggagcc cgcggagcca
      721 gttcagatag agccagctct gaccgccgaa agggcacctg cccatcatcc cgagaacaac
      781 gagtctcagc ttacgattca ttttgccggg cggccctttt gctcgcctgc tcgttcctgc
      841 cacagtcgac aggaagaaac aagatcatac gagggcacgt gttttttctt cctgagcgct
      901 gaattgggtg cgataaagtt tggcaaaatc gcctcggagt cgcatgagct cttcgaatgt
      961 tccctgctcg actatttttc ctccgcgcac aacaataatc agatcggcct ggcggaccgt
     1021 ggagaggcgg tgagcaataa tgaaagtggt gcgacctgcg gtcagctccc ggaggccttc
     1081 catgattaga gcctcggttt cagcatcgac cgaggaagtg ggctcatcta gaatcagaat
     1141 gggagcattg atcaggatcg ctcgggcgat cgttagtcgt tgcttctcac cttcggatag
     1201 cgtagcgcct tgttctccga ctaccgtgtc ataaccttca ggaagcgcca caatagtctc
     1261 gtggatgcga gccagccggg cggcgtttgc cacttcctct tcggtggctt cgggccgacc
     1321 atacatcaag ttttcccgga tagtgagtgg aaagaccagc ggaggctgga gcaccatagc
     1381 gatctgacgg cgcaacgacc tgagctgata ctcgcgtata tcaacgccat cgatagtgac
     1441 ccggccgctt ttgggatcat aaaagcgcgg cagcaagctt accaaagttg atttgccggc
     1501 tccggtacgg ccgacgatcg ccactttttg gcccgggcgg acgtttaagt cgacatgtct
     1561 caagacgggc tggccggcac tatagtggaa agagacttcg ctccaggcga cttcccccct
     1621 ggctccgcct ttaggaaaaa ctctggagcc ttcctcgagc tctccttcta cctccatgat
     1681 ttcgaacact ctttggagac tcaccttggc tccttcaata gagccgtacg cctgggcgat
     1741 ggagtctatg gctccataga gcgaggtgag ataagccgta aagacgatca gcgcgccgat
     1801 ggtgagcgta ccggcgagaa cgtgacgggc ccccacccaa atgacaaccg cggtgccaat
     1861 cgcgatgacg acacttaccg tccctgaata aaagttctgc agcaggtaaa agtgaagttc
     1921 agccttcaag cttttttggc ttagggccat aaaacgacga tgttcctctt cttctttcgt
     1981 aaacgcttga gtaacacgga gagccggaat ggcccactgc acgagggaga aaacgctgct
     2041 tttttgctgg tgcatgcggg tggcggaggc gatgagccaa ccacgcagag gcgagatcat
     2101 cacgaagaca aataaaatcg gacatatgat gagagccaga aaagtgagga gcggatccat
     2161 gcggatcatc accaaaaaca tgccgacgaa aagcgtaaga gagcacaaca cagggaaaag
     2221 gccgttcatc gtgagtgtct ggatcgcgta ggtatcggag gtcaaccgat agagtaggtc
     2281 gccgacctga cggcggtgat aaaaagacag agaaagacgc tggatttgac tgtagagatc
     2341 ccgacgcaga tcgttgacca tcttctgccc gattctgatc gtcgtatagt cattgagcat
     2401 gcgcagtccc ccgagtagaa gatagacaac gaccagaccc gcgcaagcga acaagagaag
     2461 gttttgtgag gaccaactaa cagcaaacgc ccagggcatg ggcgtttcag tcatgacgtg
     2521 gtcgatgata atcttcagcg gccaaggctt gaggagctcg agcgcgctga tgaaagcgac
     2581 ctgcgtcagg gcgacgacaa atgttggaag gtacgggcgc aggtaggaac gaaccctaag
     2641 tctcatcggc ggaatgttct cacagcgctt cttccgacgg cttttgcgcc ggcacgaaat
     2701 cgagcttgtg cgctagagat tccaggacgt ggtagagcac gcgaccgaga cggaattttt
     2761 ggtgtaaaat cgcgcccccg tgaatcaaac ccgtgaccaa agctgctacg gcgatctgcg
     2821 gcatcgacag gaagactgca accgtcatca acacggcata aagggaaaga caagtaaaag
     2881 acatccttga catccgcagc gcgcagcgca ctctcaacaa acgcttcccg ctgccgtgat
     2941 tttccgtagc cgctcggatc tgcgctttcg cccacggacc ttgacaaatt tctagatccc
     3001 agtgactcca tccctgatcc acggctatga gatatttgcg cggcagtaaa aaatccatga
     3061 tcgaataaag caaactctct ttctcccgtc ct
//
LOCUS       DASSWZ010000005         4806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_325654, whole genome shotgun sequence.
ACCESSION   DASSWZ010000005 DASSWZ010000000
VERSION     DASSWZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325654"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1220)
                     /locus_tag="VFN58_00100"
     CDS             complement(81..1220)
                     /locus_tag="VFN58_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET8916416.1"
                     /translation="MKRWHIALLAVVILLSTSIVIGYQLGVRLLQDRIVAALGAGSSV
                     AELKVNWFSLDVFGVTIAAPKDWPTGRTFRAARVRIIPSLRTLLTDQIHISSIVFEEP
                     YVSVMRTSGKWVIFPGLTEAREGKKNAEASRANFSPRAVTISKIELQDGIVELFDATV
                     SRPPLKTRLERVGAVIRNVTAPSLQTRTHIESAAIVKGIRRDGRARVSGWVGPNRGDS
                     SSQVTLDAVDLVSLQPYLVKIGEGQVRKGTLDLRLNSDIRANRLDGKGKIIIRDLEFA
                     QSRGYLETFMGIPRSALINFLKNNENAIDVDFVLTGDTSNPSFSINEAIATRVAMGMA
                     AELGVSIRGLAEDFGTLGRRSVEGAAGVAEGVGSVVRRLFGGSRK"
     gene            1428..2840
                     /locus_tag="VFN58_00105"
     CDS             1428..2840
                     /locus_tag="VFN58_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET8916417.1"
                     /translation="MIESNKYNIIEVNMMPLGLTGIKDRSIIMAMFWLMFVSILIPHL
                     SFAQDKVIATYTLPDTPIKAFQNAVLPGSVANDRKVLLGSVGSDLWHGPRDPRDEFWM
                     ITDRGPNGQIAVDGKNRRTFWVPEFNPTIVRVKTEGKAIKILDAIPIVGQSGKPVTGL
                     PNLKDVDEAPYNYSAQELLPLNPNGLDTEGLVRTTAGDFWISEEYSPSIVHVDRTGKV
                     IKRYIPEGLNLEGTDYPVAKVLPSIYGKRKINRGFEGIALSGDEKTLYMVLQSPLLNP
                     DRKTGEASRNTRVLVFDIPSEKVTAEYVYRFDVSKEFDPNPKNTPDEMKLSGVIAMNP
                     TTLLILERTDLVAKLYSVDLSQATNVLGSKWNDTKTAPALEALADPAAAEVRVLPKSL
                     VLDLSSLAGMPEKIEGIALIDQSTIAVSNDNDFDSEESKYDAEGNNIGKGKISQILGI
                     SLAKPLPLQQSTVAKASESR"
     gene            complement(2918..3937)
                     /locus_tag="VFN58_00110"
     CDS             complement(2918..3937)
                     /locus_tag="VFN58_00110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8916418.1"
                     /translation="MKFGAHFLPTYVPELDGPVTQFYRRMFEQIEALERLGFDRAWVT
                     EHHFGDYGGSIPHPPTFLAAIACKTSRIRLGVAVAVLPLNNPIYLAESYGMVDVVSNG
                     RLDFGIGKGSEPIEYVRFGARREEASQRMTEGTEILRQAWSDEPVNFHGEFFNYDNVR
                     VLPKPVQRPHPPIWVGATRTEETFRWAGENGFNLMTIPFVHPSMGALHGLVEIYREAL
                     ARAGHDSATRQILAKFHIYVSDSLAQGIREASPYMKNYSAIHAAVDPNRKLTNRDIVL
                     DMERGFIIVGDPQRCIDTIRRWQEEVGLTTFSFTFHFGGMPQEMALKNIRLFAERVMP
                     ALDGA"
     gene            complement(4414..4659)
                     /locus_tag="VFN58_00115"
                     /pseudo
     CDS             complement(4414..4659)
                     /locus_tag="VFN58_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167925.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1114 a         1357 c         1248 g         1087 t
ORIGIN      
        1 caccagctga ttcaataccg gtgtattgga aggcaaaagt cccgcagcgg cgccgtcgta
       61 cagggtcgtc aggagatcac ttatttcctg ctaccgccaa agagacgcct gacaacagag
      121 ccgacaccct cggctacccc agcagcgccc tctacgctcc gtctgccgag cgttccaaaa
      181 tcttcagcca aacctcggat gctgacgccg agttccgccg ccatccccat cgcaacgcgg
      241 gtagcgatgg cttcgttgat cgagaagctg ggattgctgg tgtcgccggt tagaacaaag
      301 tccacgtcga tggcattctc attattttta agaaagttga tcagcgcgct gcgaggaatc
      361 cccatgaagg tctccaggta gcctcgcgac tgtgcaaatt cgagatctct gatgatgatc
      421 ttgccttttc cgtccagcct gttggcccgg atatcagagt tgagtcgcaa gtccaacgta
      481 ccttttctga cctgtccctc gccgatcttg acaagatagg gttggagact caccagatcc
      541 actgcatcaa gtgtgacttg cgatgaagag tctccccgat tgggcccgac ccagccggaa
      601 acccttgctc tgccgtcgcg cctgatcccc ttcacgatgg ctgccgattc aatgtgagtt
      661 ctggtctgca atgatggagc ggttacgtta cggataacgg cacccacccg ctcaagccgg
      721 gttttgagcg gtgggcggct caccgttgcg tcgaaaagct ctacgattcc gtcctgtaac
      781 tcgattttag agatcgtcac cgcgcgaggg gaaaaattgg cacggctcgc ctcggcgttc
      841 ttcttgcctt cccgcgcttc cgtcagcccc ggaaatatga cccactttcc actcgtgcgc
      901 atcacggaaa cgtaaggttc ttcaaaaacg atggaagaga tatggatctg atcggtcaaa
      961 agagttcgca gactcggaat gattctgact cgtgctgcgc gaaaggtgcg ccccgttggc
     1021 cagtctttag gggccgcgat agtgacccca aacacatcga gggaaaacca gtttaccttg
     1081 agctctgcga cactgctgcc ggcccccaga gcggccacaa tcctgtcctg aagcagccgc
     1141 acgcccagct gataccctat gacaatactg gtgctgagaa gaatcaccac ggcgagaaga
     1201 gcgatatgcc aacgcttcat aaaatacccg caaggaaaga tattgtgaca gcggtaattc
     1261 tcgcctgcaa attcgctcta tggcagttgc cctaccggcc cggcggatga aacagcgacg
     1321 acctagattt gaaagcactt tgaatccttt ctgatccttc tccgtaggtt tctgtaacct
     1381 tagctttatc cgctcttaac ctttcgttaa cacaaccgct aattactgtg atagaaagca
     1441 ataagtacaa tatcatcgag gtaaacatga tgccgttggg tctaactgga attaaagacc
     1501 gtagtattat tatggctatg ttctggctta tgttcgtcag tatcctcatc ccgcatctat
     1561 ccttcgccca agacaaagtc attgccacct acacgctacc cgataccccc atcaaggcat
     1621 ttcaaaacgc cgtgcttccg ggaagtgtgg ccaacgatcg aaaagttctc ttaggttccg
     1681 tgggcagcga tttatggcac ggccctagag atccgcgcga tgaattttgg atgatcaccg
     1741 accgtgggcc caatgggcaa atcgccgttg atgggaaaaa tcgtcggaca ttttgggtgc
     1801 ctgaattcaa tccgacgatc gtcagggtca agaccgaagg caaagcgatc aaaattctgg
     1861 acgcgattcc gatcgtcggc caatcgggta agccggtgac cggcttaccc aatctgaaag
     1921 acgtcgacga agcgccgtac aattattcgg cgcaggagct tttgccgctg aatcccaacg
     1981 gcctcgacac cgaaggcctg gttcgcacta ccgcgggcga cttctggatc tccgaggagt
     2041 acagcccgtc gatcgtgcac gtcgatcgaa ccgggaaagt catcaaacgc tacattccgg
     2101 agggtctcaa tctcgagggc acggactatc ccgtcgcaaa ggttttgccg tcgatttacg
     2161 gcaaaagaaa gatcaaccgt ggcttcgagg ggatcgcgct cagcggcgac gaaaagaccc
     2221 tttatatggt cttgcaaagc ccgcttttga atccggacag aaagaccggc gaggcgtcgc
     2281 gcaacacccg agtgctcgtg ttcgacatcc cgagcgagaa agttacggcc gagtacgtct
     2341 atcgattcga cgtatcgaag gaattcgacc ccaatccgaa gaatacccct gacgagatga
     2401 agctctccgg agtgatcgcg atgaatccca ccacgctgct tattcttgag cgtaccgatc
     2461 tggtcgcgaa gctatacagt gtggatctca gtcaggcgac caacgttctt ggcagcaaat
     2521 ggaacgatac caagactgcg cccgccctcg aagcgctcgc cgatccggcg gcagcggaag
     2581 tccgcgtgct gccgaaatcg ctcgtgctcg atctcagctc gctagcgggt atgccagaga
     2641 agatcgaagg catcgccttg atcgaccaga gcaccatcgc cgtctctaac gacaatgact
     2701 tcgacagcga ggaaagcaaa tacgatgctg aaggcaacaa catcggcaag ggaaagataa
     2761 gccagattct cggcatttct ctcgccaagc cgttgccatt acagcagtct acggtagcga
     2821 aagcttccga atcccgttaa aagaaccgat cggtgctaca taaactttgt tgaagtagtt
     2881 ccagtatcgt cctgctctac gttacagttt tttccgatca ggccccgtcc aaggctggca
     2941 tgactcgctc ggcaaacagc cggatattct tgagcgccat ctcctgtggc atgccgccga
     3001 aatggaaggt aaaggaaaac gtcgtgagac cgacttcctc ctgccagcgg cggatcgtgt
     3061 ctatgcagcg ctgcggatcg ccgacgatga taaatccgcg ttccatatct aaaacaatat
     3121 cgcgattggt gagtttacga ttagggtcga cggcagcatg aatagcggag tagttcttca
     3181 tatagggaga tgcttcgcgt atcccctgcg ccaaactatc cgacacatag atgtgaaact
     3241 tcgccaaaat ctgccgcgtt gctgaatcgt gtccggcccg cgccagcgcc tcgcgataaa
     3301 tctcgacgag tccgtgtaat gcccccatgc tgggatgcac gaagggaatg gtcattagat
     3361 tgaatccgtt ttcccccgcc cagcgaaatg tttcttcggt gcgagtcgct cccacccaga
     3421 tcggcgggtg cggtcgctgc accggcttgg gcagcacgcg aacattgtcg tagttgaaga
     3481 attccccgtg gaagttgacc ggttcgtccg accacgcctg gcgcaggatt tcagttccct
     3541 cagtcatgcg ctggctggcc tcctcccggc gcgcgccgaa tcgcacgtac tcgatgggct
     3601 cgctgccctt gccgatgcca aagtccaaac ggccattgga gacgacgtcg accatgccgt
     3661 atgattccgc caagtaaata ggattgttca acggcagcac ggcgaccgcc acgcccaagc
     3721 gaatgcggga ggttttgcac gcgatcgccg ccaagaacgt cggcgggtgc gggatcgaac
     3781 cgccataatc gccgaagtga tgttcggtca cccaagcgcg gtcaaatcca agtctctcca
     3841 acgcctctat ttgctcaaac atgcgccggt agaattgggt taccggcccg tcaagttccg
     3901 gcacataagt gggcagaaag tgcgcgccaa atttcatgct gcctccgact gttgttttct
     3961 ccattttctg agtgaaggta gagaaatcgg ttccaccata gtcagcaagc cagaacctgt
     4021 caacgcaacg atgaatcaac ggtggtggca ctggggtcac ttgggagacg gcaggtcaag
     4081 aggcacagac attcctgtgg aaatttttct tgttcccttt tttcaactcg cgtgatccgt
     4141 gatctctgag aacgcgcccc tgttttcttt tcaatcggtg tcgtttttgg tccgtaccag
     4201 tcacccatca ggatcaaggc aatgggagat tctaagagag aaatcttccc gcccctcgca
     4261 agtctacttg ccccagaaaa ctgtcggtac cacaccgcat ccaaggtttt tggattcaca
     4321 gatcctgtgg ctctccctgt tcgggggcca acccttaact ggctcacggc gtaggcgtac
     4381 catcgaatca cagccaccat cgagttgtct ctatcaggga tgagacagcg gccaatgatc
     4441 cctcatgatg tagtaacagg cccgacacag cttgtgcgcc accgctttga gcgccaccac
     4501 ggggtggctc ttggctttct ttcgttggta gaaactgcga accttgggac taaaccggat
     4561 cgcaaagttg gccgtttcca caaacgccca gctcagatac ttattcccgt tcttggtgtt
     4621 gccgctgcct ttgcgtctgc cgttggaaag tttctggctg ccctggcaaa tccgcttgtt
     4681 ttttggcact gagaccattt agactccaag ctggtttgtg atattttgct gactctgaag
     4741 ttagaccaag gaggatgaga gatgtctatg aattggggaa tggcaaatcg tttagcccgg
     4801 atcatt
//
LOCUS       DASSWZ010000006         4317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1401141, whole genome shotgun sequence.
ACCESSION   DASSWZ010000006 DASSWZ010000000
VERSION     DASSWZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401141"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFN58_00120"
     CDS             complement(<1..441)
                     /locus_tag="VFN58_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916419.1"
                     /translation="MTTDRRPARDKLRREYDSSHGLEKIRAPACANLAPLFGALEAAR
                     DAVAREPMRNVCTRFLASLSAFYGIPSPSLKLLGPRPHSTYEGRLANELFGDYQIMGA
                     KIRLWTRTPMKRQWTSSKTILSTLCHEFMHHLDVTSLGFPNSFHT"
     gene            complement(530..1420)
                     /locus_tag="VFN58_00125"
     CDS             complement(530..1420)
                     /locus_tag="VFN58_00125"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HET8916420.1"
                     /translation="MREDLELLRGGIDIHVHSGPDLYPRIQDHVELALSARTAGMRAL
                     VIKSHNFPTAAHAVATSKAVSDIDVFGGIALNLHVGGINPIAVEAAIKYGAKQVWFPT
                     VDSCNHARLVGGEVGQHGKGLVIPGGLSEYTVKHPRIRIVENDGSVSRDVHEILRLIA
                     AANIILNVGHTSFEEMEALLIAGRAAGVKKMVVDHPFFSQLTLQQQERLAALGAFINY
                     TAGEILPRWWRVSVEDFAAGIRRIGVTRTILSSDAGQLHNPPPVEALRITVQLLLEEG
                     FSTDEIRTLLHENPAKLLYD"
     gene            complement(1541..1966)
                     /locus_tag="VFN58_00130"
     CDS             complement(1541..1966)
                     /locus_tag="VFN58_00130"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8916421.1"
                     /translation="MPIEIVDMVKKAHELKKRKVLLNTKRFHAWVHYYPNPGDRDDMH
                     CHNADQTFYVIEGECTMHFPDGGKAVMKPGMAALITGGSFYQLENTGPGPMVLMGNRS
                     GPSEDIQHINYELRKDIKTLTPEERERIQHGGAVQTPEG"
     gene            complement(2466..4082)
                     /locus_tag="VFN58_00135"
     CDS             complement(2466..4082)
                     /locus_tag="VFN58_00135"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HET8916422.1"
                     /translation="MSLRAVDSQRSFYHTDYLAGNLFGPANRYRLFREKIWPKLLELA
                     PKLEALYCAENGRPPIDPVLLCGVTLLQFMEKVADRRASDQVVYHLGWKYALDLDLDD
                     DGFHSTVLVYFRDRLEEKKAERLIFDGMVDLLIELGLVKRKGKQRLDSTHIVGYVKAM
                     SWMECAIETLSLALEDLEREVGQNKRPEFWGRLWEFYVESNLDWQLSKTEQANRHRQC
                     GQDIRELLEWIDRTDPKLAEREAVKLLRRVFGEQFEVVEGKLDLSTQRPSRAVQNPHD
                     PDAHYADKKTKQWTGYKVHVAETVDPEVPIKEKGEPAEHFITEMFTTEAAQDEMAGLT
                     EVLKREQQHHEITPEAMYTDAGYVTENTLTQAEQNGIELLGPTRPNPHKGPYNTDAFQ
                     VDVDKRQAICPQGKLSTQCSRIKDSYMGTEYYRIEWGNQCDSCPVQKQCTRSKGGRRT
                     LVVGLRHDLVQQRRREMTQTGFSKSMHPRNGIEGTHSELVRGHGLRRTKYRGLSRVGL
                     SHYFMGAACNVKRYLNLLAFQMRTPAPSPA"
BASE COUNT          928 a         1245 c         1069 g         1075 t
ORIGIN      
        1 ggtatgaaag ctgttcggaa atccgagaga agtcacatcc aagtggtgca tgaattcatg
       61 gcaaagcgta gacaatatcg tcttactcga tgtccactgc cttttcattg gggtccgcgt
      121 ccaaagtcga atttttgcac ccatgatctg gtagtcgccg aagagttcat tggccaagcg
      181 cccttcgtaa gtcgaatgcg gacgaggccc gagcaatttt aagctcggcg aaggaatgcc
      241 gtaaaaggct gaaagcgaag cgagaaaccg cgtgcagaca tttctcattg gttcgcgggc
      301 taccgcatcc ctggccgctt ccagcgcgcc aaacagagga gccaagttcg cacaagccgg
      361 cgcacgaatt ttctccaggc catgactact atcgtattcg cggcgtaatt tgtcgcgcgc
      421 cggccgccga tctgtggtca tagttttatc tgtgaccgct catttcgatt cttcaagccg
      481 tgcaagggtt caggctaaaa gtagcctgtc ccctttatgc cccttcattc tagtcgtaca
      541 acagcttcgc cgggttctcg tgaagtaacg ttcggatctc atctgtggaa aaaccttcct
      601 ccaagagcag ttgcacggtg atgcgcagtg cctctacggg aggtggattg tggagctggc
      661 cggcgtcgct tgagaggatc gtgcgcgtca cgccgatccg gcgaatgccc gcggcaaaat
      721 cctccaccga cacgcgccac cacctgggaa gtatctcgcc tgccgtgtag ttgataaaag
      781 cgccgagggc ggcaagtctt tcttgttgct gtaacgtgag ctgagaaaaa aacggatgat
      841 ccacaaccat tttctttacg cccgccgcgc ggcccgcaat cagtaacgct tccatctcct
      901 caaaggacgt atggcccacg ttcaggatga tattggccgc cgcgatgaga cgaaggattt
      961 catgaacgtc cctcgagacc gagccgtcgt tttccacaat ccggatgcgc gggtgtttta
     1021 ccgtgtactc cgaaagccca cctgggatca ccaaaccttt gccatgctgt cccacttcgc
     1081 cgcccacaag cctggcatgg ttgcatgagt ctacggtcgg aaaccaaact tgcttcgcgc
     1141 cgtacttgat ggcggcttct acagcgattg gattaatccc gcccacatgc aggttaagtg
     1201 cgattccgcc aaagacatca atgtccgaca cggccttgga ggtcgccacc gcatgcgcgg
     1261 ccgtcggaaa attatgggat ttgatcacca gcgcgcgcat gcctgcggtc cgggccgata
     1321 acgcgagttc cacatggtcc tggatccttg gatagagatc aggcccggaa tgcacatgga
     1381 tatcgatccc gccccgcaat aactcaagat cctcacgcat acgctcgcct ccccttaacc
     1441 tccctcccag atttttggcg cgcgacgatc cgcggtttca aattgccggc cgcgatttca
     1501 acccgatagt aacgtcggcc cggtagaaac cggacgtgca ctaaccttcc ggcgtctgga
     1561 cggcgccgcc gtgttggatt ctttcccgtt cctccggggt caaagtctta atgtcttttc
     1621 ttagttcata gttgatgtgc tggatatctt cagacggtcc ggaacggttg cccataagaa
     1681 ccatcggacc gggtcctgtg ttttccaact gataaaacga cccgcccgtg atcagcgccg
     1741 ccatgccggg tttcattacc gctttgccgc cgtccggaaa atgcatcgtg cattctccct
     1801 cgatgacata aaaagtctgg tccgcattgt ggcagtgcat atcgtctctg tccccgggat
     1861 ttgggtagta gtggacccag gcatgaaaac gcttcgtgtt gaggagcact tttctcttct
     1921 tgagctcatg agccttcttg accatatcga cgatctcgat tggcatacat tccccctttt
     1981 tcaaagttgc gtctgatctc tcaatcgaat actcgctact aaaggcaacg tcaaggagtg
     2041 agagggcggg atcgataaaa aacgaattct gctattctgc aactctgttg actgaaagag
     2101 ttgaaccacg accccttgaa tggcattcga aagacttccg tgaatagaac gtaggtcaga
     2161 tcacaagccc caagcgtttc agccgctggg ttagctggct cagctgcgcg cttccgacgt
     2221 ccgtcgtcca gaacactatg atctcctcga cggcttggct ggcgtgagtt cgttgttggc
     2281 ccgggattaa acctgtctta tcctttgata gaagtggaat gttagattgc gattctgctg
     2341 aaaaacccgt caatttaatt tgccatcgcc ggtagcaaac gctacggata gccaaagagt
     2401 tttgcggcag gtaaattttc acccgcaacg ctgcgatagc cagtaaaagg aacggccttg
     2461 agctctcaag ccgggctcgg agccggcgtt ctcatctgga acgcgagtaa atttaggtat
     2521 cgtttcacgt tgcaggccgc gcccatgaaa tagtgcgata gaccgacccg gcttaagcct
     2581 cgatatttcg tccgtctcaa gccgtgcccg cgcaccagct cgctgtgggt cccttcgatg
     2641 ccgtttctcg gatgcatgct ctttgagaat ccagtctgtg tcatttctct gcgccgctgc
     2701 tgcaccagat cgtggcgcag tccaaccact aaagttctcc gtccaccctt ggaacgcgta
     2761 cattgttttt gtacaggaca gctatcacat tgattgcccc actcgatgcg atagtactcg
     2821 gttcccatat agctatcttt aatgcggctg cattgcgtgc tgagtttgcc ctggggacaa
     2881 atcgcttgac gcttgtcgac atcaacttga aacgcatcgg tattgtacgg ccccttgtgc
     2941 ggattgggac gcgtcggccc taataactcg atgccgtttt gttccgcctg cgttagggta
     3001 ttctcagtca cataccctgc atccgtgtac atcgcctcag gtgtgatctc gtggtgctgt
     3061 tgttctcttt tgagcacctc ggttaaaccg gccatctcgt cctgagctgc ttcggtggtg
     3121 aacatctcgg taatgaaatg ttcggcgggt tcgccctttt ccttgatcgg cacttccgga
     3181 tcgaccgtct cggccacgtg tactttgtag ccagtccact gcttggtctt cttatcggcg
     3241 taatgggcat cgggatcatg cgggttctgt accgcgcgcg aaggtcgctg ggtactgaga
     3301 tcaagcttcc cctcgaccac ttcgaactgc tcgccaaaga ctcgccgcag aagtttcacc
     3361 gcttcgcgtt cagcgagctt aggatcagtt ctgtcgatcc actcaagcag ctctctgatg
     3421 tcctgcccac attgccgatg gcggttggct tgctcggttt tgcttaactg ccaatccaga
     3481 ttgctctcta cataaaactc ccacaatcgc ccccaaaact cggggcgctt gttctgcccg
     3541 acttcacgtt ccaaatcctc caacgccagg cttagcgtct cgatcgcgca ctccatccag
     3601 ctcatcgctt tgacgtatcc cacgatatgg gtgctatcga ggcgctgctt gcccttgcgc
     3661 tttaccaacc ccagctcaat caataaatcc accatcccgt caaaaatcag ccgctcggct
     3721 tttttctctt ccagccggtc gcgaaaataa accaacaccg tggaatgaaa cccgtcgtca
     3781 tccaggtcca aatccagggc gtacttccag cccagatgat agacgacctg atcgctggct
     3841 cttcggtctg ccactttctc cataaactgg agaagcgtca ccccgcaaag tagcaccgga
     3901 tcgatcggcg gccgcccgtt ctcggcgcag taaagcgctt ccagcttagg cgcaagttcc
     3961 aaaagcttgg gccaaatctt ttctcgaaac agacggtagc ggttggctgg accgaaaaga
     4021 tttccagcaa gataatcggt gtgataaaag cttcgctgac tgtcgacggc tcttagactc
     4081 atcgcaaaac tttgaagccc actgcgccga ttgtcaacgt ttatttcttg aatatgacta
     4141 aataccaaat gggtttttca gcagaatcaa tatctagacc tgtcccggaa aagtgcgctg
     4201 cgtttcttcg tcgatcacag taaaccgttc caatggttcc aatcgttcga tcgcttcact
     4261 cagttccacg cgcggccaat tccctggtta actcgaaaat tcatcaggtc tctttcg
//
LOCUS       DASSWZ010000007         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_750182, whole genome shotgun sequence.
ACCESSION   DASSWZ010000007 DASSWZ010000000
VERSION     DASSWZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750182"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1330
                     /locus_tag="VFN58_00140"
     CDS             116..1330
                     /locus_tag="VFN58_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8916423.1"
                     /translation="MKDRAANITLSDLAHVDRGTAAGEWFRRYWLVVGTIKELRDIPV
                     RVRILGEDLVLFRDLKGDIGLIGLHCPHRGSSLEYGDIEERGIRCPYHGWLFDVTGNC
                     LQQPGEPKGSEYHRKVKHLSYPVRELGGLIFAYLGPDGDNPPPLPKYAPLTDGGGQRY
                     VESIRYYDYNWFNFIENGVDPVHFSVLHRSDPGDGTWRSWFFNFHDIPHFDAVETSYG
                     VKVISRKPGPTADTEYVDEKSVALPSIIQIGDLEFTHFHKPASALGEGSHVEHIMFAT
                     PNDDDHFMLFTADCYTGPDPDFFTKLAETRPPPVRQDKKEYDHRKHMPFRGSVRHEDI
                     VTQGTQGSIDEREEHLAASDRGVILLRKTILRAMETAQKGGLPQGVRAMEQAEEIFKI
                     QSFTGIRPRGVF"
     gene            1340..>2314
                     /locus_tag="VFN58_00145"
     CDS             1340..>2314
                     /locus_tag="VFN58_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916424.1"
                     /translation="MKFPRLLTHTLAVLFLLSLSQAQAAPVRVAYSAISGAMAPLWVT
                     QEGGYFRREGLDIELLYIGGGSLLIQSILGGDVQFAYGPSVPVVNAALRGSDLVFIAN
                     TGDTLIFSVMTKPEIKEPIDLKSKRVGVTRLGGSADLALEFALKRWGLQRGRDLIVLQ
                     TGGLPESLAALRSGAVDGAVLSPPNNLLAKKAGLRELVDVGQLGIIFPNTPLSTTRSY
                     IKSNRDALIRFLRALGEGQHRLRTDKDFSVNVLSKYTKTTDPEILGELYRIYGVRYTG
                     QQIPYVRPEGVEERLKGMDSKEARQTKPADFIDNSLLREVEQTGFFRKW"
BASE COUNT          534 a          615 c          648 g          517 t
ORIGIN      
        1 cgaaagatgt tgggtttcac agcgagatat ctcttcgaag atcgatgaac cgtgtcagaa
       61 ttagaataag tgtggtactt gtactgcgcg catgtcgtaa ggaacggaga cgcatgtgaa
      121 agaccgtgcc gccaacatta ccctctctga ccttgctcat gtagaccgcg gaacagcggc
      181 gggagagtgg ttccgccgct actggctggt tgtcggcact ataaaagagc tccgcgacat
      241 tcccgtgcgc gtaagaattt tgggcgaaga tctagtactt tttcgcgacc taaaaggaga
      301 catcggtctt attggcctgc attgccccca ccggggcagc tctctcgaat acggggacat
      361 tgaagagcga ggcattcgtt gcccctatca cgggtggctg ttcgacgtaa cggggaattg
      421 tctccaacaa ccgggagagc caaagggcag cgagtatcat agaaaggtca agcacctttc
      481 ctatccggtg cgggagctgg gcggactcat cttcgcctac ctgggaccgg acggagacaa
      541 tcctccgccg cttcccaaat atgccccgct taccgacggc gggggtcagc gctacgtcga
      601 gtcgattcgc tactacgatt acaattggtt caattttatc gaaaacggcg tcgatccggt
      661 gcatttctcc gtgctccatc gtagcgaccc cggcgacgga acgtggcgga gctggttttt
      721 caactttcat gatattcccc attttgatgc ggtcgagacg tcctatggag tgaaggtgat
      781 ctcacgcaag cctggtccga ctgcggatac ggaatatgtg gatgagaaaa gcgtggctct
      841 gccgagtatt attcaaatcg gcgatctgga gttcacccat tttcacaagc cggcttcggc
      901 attgggagag ggcagtcacg tcgagcatat catgttcgcc acgcctaacg acgacgatca
      961 tttcatgctc ttcactgctg attgctacac cgggccggac cccgattttt ttaccaagct
     1021 ggccgaaacc cgtccgcctc ccgtcaggca agacaagaaa gagtatgacc accggaagca
     1081 catgccgttc cgggggagcg ttcgtcacga agacattgtg actcagggaa ctcaaggttc
     1141 cattgacgaa cgggaggagc atctcgccgc atcggatcgc ggagttattc tgttgcgcaa
     1201 aactattttg cgggcgatgg aaacggcgca aaagggcggg ttgccacaag gcgttcgcgc
     1261 catggaacag gccgaagaga tcttcaagat ccaatctttc actggtatta gaccaagggg
     1321 agtcttctaa accacacgca tgaaattccc acgtttattg acgcatacgc tagccgtact
     1381 gtttcttctc tctctgagcc aggcgcaggc ggctcccgtt cgtgtcgcgt atagcgccat
     1441 cagcggcgcg atggcaccgc tgtgggtcac tcaagaaggc ggatattttc gacgagaagg
     1501 tttggacatt gagctccttt atattggcgg tggttcgttg ctgatccagt cgattctggg
     1561 cggagacgtc caattcgcct acggcccttc ggtaccggtg gttaatgcgg ctctcagagg
     1621 ctcggatcta gtgtttatcg ccaacactgg agacaccttg atcttctcgg tcatgaccaa
     1681 accggaaatc aaagagccca tcgatttaaa aagcaaacgg gtcggcgtca cacgccttgg
     1741 aggctcggcc gacctcgccc tggaatttgc cctgaagcgc tggggactcc aaagaggccg
     1801 cgacctgatc gttttgcaga ccggcgggct tccggagagt ctcgcagctc tgcgctccgg
     1861 cgccgtggat ggagccgtgc tgtcccctcc caataattta ctcgccaaaa aagccgggct
     1921 tagagagctg gtcgacgtcg gtcagctggg gattattttc ccgaacacgc cgctttctac
     1981 gacccgatcg tatatcaaat caaaccggga cgccctgata cgatttctcc gggccttggg
     2041 cgaaggacaa catcggctga gaactgacaa agactttagt gtgaacgtct tgtcgaagta
     2101 caccaagacc acggatccag agatattggg ggagctatac cggatttatg gtgtccgtta
     2161 taccggacag caaattcctt acgtgcgccc ggagggcgtt gaagaaagac tcaagggaat
     2221 ggattccaaa gaagcccgtc agacaaaacc ggcggacttc atcgataaca gccttctcag
     2281 ggaagtggag caaaccggct tttttcggaa atgg
//
LOCUS       DASSWZ010000008         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_580398, whole genome shotgun sequence.
ACCESSION   DASSWZ010000008 DASSWZ010000000
VERSION     DASSWZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580398"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..880)
                     /locus_tag="VFN58_00150"
     CDS             complement(134..880)
                     /locus_tag="VFN58_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8916425.1"
                     /translation="MSQASSQKMALRAEGLWFAYRETDWILKDVCLSVPNGQITIIMG
                     PSGTGKTTLLKILAGILAPSRGHVEVLNQDIAKHSQRSLASLIGYIPQQLGLVRNLTA
                     LENVLMGALGRCGSGSALLGLFPGEEVKKAHAALDLMGIAHKANEKVFRLSGGERQRV
                     AIARTLLQHPRMVVADEFVSDLDMALALEILSRIRRAAQEQITFVMSMHRQGLVREFA
                     ENVVTLREGQVAPGFIPVLTQGSAAVEMVQ"
     gene            complement(877..1824)
                     /gene="phnD"
                     /locus_tag="VFN58_00155"
     CDS             complement(877..1824)
                     /gene="phnD"
                     /locus_tag="VFN58_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8916426.1"
                     /translation="MRAAGERIHKLSFLVTLLILLSMGVTGRAIAQGKLVVAIQPSVS
                     SDEMLKKAQPLEKFLRDGLGGKTDVQVYVPSSFAAVVESLRFGHAQVAFMSAWPAQLA
                     VQLGGADVALAEVREVLHGTKKVEAPYYFSYWIVQKEAPYQNLQALKGKTACFPSPIS
                     TSGYVAPMGRLVELNLIPTPEGKEGDPKAFFKDVVFGGGYGQCAQALKQGQVDVTVIA
                     GDVPVKLYNEVLSTTRTIEEQGPIPSHAVVLSKELKEPLRSQTIQTIMKLSAPEHRDL
                     MRSFISSIFVGFKPTTAAEHLGSLQRYLKLAGLKYTERL"
     gene            1971..>2574
                     /locus_tag="VFN58_00160"
     CDS             1971..>2574
                     /locus_tag="VFN58_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET8916427.1"
                     /translation="MSNSKITQAIEDYLRTIYALEEEVQPVIAARVAEEVGVTPSTMV
                     STLRRLEGEGYLKVVRRKEIHLTAEGKQVAERILRRHFLLERFLTDLLGLDWVKAHQE
                     AHRLEHALSQEVEDRLAKLLGYPTTCPHGNPIPSKDSQRSPKSKGTPLHSIAAGKEVE
                     LDYITEGGERDARLLGFLQEHRLVPGTKVRVLDVAPSMGVM"
BASE COUNT          588 a          687 c          722 g          577 t
ORIGIN      
        1 ggaaccatat cagacgtaag aataccgaga ttgcgtaatc cacggatcaa tttcccgctg
       61 tcaaagaacc ctagctcatt caggacgata cccaagagca gggtgaggag agcaatgata
      121 aggagattcg tcttcattgc accatctcga cggcggccga tccctgcgtc agcaccggaa
      181 tgaatccggg cgcgacctga ccctcccgaa gcgttacgac gttctcggcg aactcgcgga
      241 cgaggccctg cctatgcatg ctcatgacaa aggtgatctg ctcctgtgcg gcacgacgga
      301 tgcgactcaa gatttccaat gccaacgcca tgtccaagtc cgagacgaac tcgtccgcta
      361 caaccatcct cggatgttgt aacagggtgc gggcgatagc cactcgctgg cgctcacctc
      421 cacttaaccg aaagaccttt tcattggcct tatgcgcgat gcccatgaga tcgagggcgg
      481 catgtgcttt cttgacttcc tcccccggaa aaagtccaag cagtgcacta ccgctgccgc
      541 agcgccccaa ggcgcccata aggacattct ccaaagcggt caaattacgc accagcccta
      601 gttgttgggg gatgtagccg atgagtgacg cgagggatcg ttgcgagtgt ttggcgatgt
      661 cttgattgag tacttcgacg tgcccgcgag agggggcaag aatgccggcc agaatcttga
      721 gcaaggtcgt cttaccggtg cccgatggtc ccatgataat cgtgatttga ccgttaggta
      781 cggagagaca aacatctttg agaatccagt cggtctctcg ataggcgaac catagccctt
      841 ccgcacgaag ggccattttt tggctgctcg cttggctcac agacgctccg tgtacttgag
      901 tcccgcaagc ttcaaatagc gctgcaaaga gcccaggtgc tcggccgctg tggttggctt
      961 gaagccgacg aagatgctcg agatgaacga gcgcatgaga tcccggtgct ccggcgcgct
     1021 taatttcatg atggtctgaa tcgtttgaga acgaagcggt tccttcagtt ctttactgag
     1081 aaccaccgcg tgcgacggta ttggaccttg ctcttcgata gtgcgtgtcg ttgagagtac
     1141 ctcgttgtag agcttcactg gtacgtcgcc ggcgatgacc gttacgtcca cctggccttg
     1201 cttcagagcc tgggcgcatt ggccgtagcc gccgccgaac accacgtcct taaagaaagc
     1261 cttggggtcg ccttcttttc cctctggcgt cgggatcaga ttcaactcca cgagacggcc
     1321 catgggcgct acatagccgg aggtcgatat ggggctggga aaacaggcgg tctttccctt
     1381 caatgcttga agattttgat aaggcgcttc cttctggaca atccagtaag aaaagtaata
     1441 aggcgcttcg acttttttcg tgccgtgaag aacttcgcgg acttcggcga gggcgacgtc
     1501 ggcgccgccc agttggaccg caagctgggc cggccaagcg gacatgaaag cgacttgagc
     1561 atgtccaaag cgcagagatt ccaccaccgc ggcaaaactg ctcggtacat acacttggac
     1621 atcggtcttt ccgcccaaac catcgcgcag gaatttttcc aggggttgcg ctttcttgag
     1681 catttcatcg ctcgatacag agggctgaat agcgacgact aatttccctt gggcgattgc
     1741 gcggcccgtg actcccatgc tcaacagaat gagaagggta acgagaaatg atagtttgtg
     1801 gattctttcg cctgcggctc gcatgctttt cctccttggt gcttgaactt tctagtcgcc
     1861 gaggctatat ggcggagaag agtcaagaca cttgacagtt ggttttaaaa agttagtatc
     1921 atctaactta agaagttcct atatggaaat aaatttactt tgtcaacccc atgtcaaatt
     1981 ctaagataac ccaagctatc gaggactatc tccggaccat ttatgccctt gaagaagagg
     2041 tccagccggt gattgccgcg cgggtcgcgg aagaggtggg cgttacgccg tcgacgatgg
     2101 tgagtacgct tcgccgattg gaaggagagg gttacctaaa ggtcgtgcgg cgcaaggaaa
     2161 ttcatttgac cgccgagggc aaacaggttg ccgaaagaat tctgcgccgc cattttcttc
     2221 tcgagcgctt cctgacagat ctgttgggat tggattgggt caaagcgcat caagaagccc
     2281 accgccttga acatgcccta tctcaggaag tggaggaccg actggccaaa ctgctgggct
     2341 atccgacgac ctgcccgcac ggtaatccca ttccgagcaa agattcacag cggtcgccga
     2401 agagcaaagg gactcccttg catagcatcg cggcagggaa agaggtcgag ttggactaca
     2461 tcaccgaggg cggggaacga gatgcgcgac ttttgggttt tttacaagag caccgtctcg
     2521 tgcctggcac caaggttcgc gtgctggatg tggcgccatc gatgggagtg atga
//
LOCUS       DASSWZ010000009         7612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1033324, whole genome shotgun sequence.
ACCESSION   DASSWZ010000009 DASSWZ010000000
VERSION     DASSWZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033324"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFN58_00165"
     CDS             <1..185
                     /locus_tag="VFN58_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916428.1"
                     /translation="DRSLEARKIDFRGNLVEVRLHSEKSLRVTKINFYPKKEKLSRSA
                     WRRKLATRGNYIRGRL"
     gene            complement(213..602)
                     /locus_tag="VFN58_00170"
     CDS             complement(213..602)
                     /locus_tag="VFN58_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916429.1"
                     /translation="MRMILPSLKERRIVDRLLSAFFHEYKPQDFKKAVAALCRFYHLK
                     NPQVDWYEYIDWGKTAGKTYENGQIYLVHPENWKKGRKYNSERKWINTVYHEMGHYVF
                     WADAENKADMFAFRMVRGLNNHKNNHR"
     gene            858..1814
                     /locus_tag="VFN58_00175"
     CDS             858..1814
                     /locus_tag="VFN58_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916430.1"
                     /translation="MIQSWNERAAYIGAWIGFNAGCLAVRIFPRPWLYRFSDTLAQIG
                     FYLFRGFRTRSIKNVGFALGEKLTEIQVEEIVRKSLRNFLRDFVEIAIALVVRAEDLR
                     SEIPIEGGENLDLALAKGQGVIVLGAHLGNFFLTGTRLAIENYPAYILVNQPRNGQFA
                     RFMDDYRLKVRQKTIHARPRQEAWRELIQVLRRNELAVVIADEYRRNNGIHVPFFGRT
                     VLARRGPATLALRTGAAVVPVYLIHDKSHGLKMIVEPELDLIRTDRDKTAIRENTLRM
                     TQWLERTVRAYPEQWNWMNIQWREDRDTAPVAKERRVQGFSS"
     gene            1834..2580
                     /locus_tag="VFN58_00180"
     CDS             1834..2580
                     /locus_tag="VFN58_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8916431.1"
                     /translation="MASSKRELDELKKKVALGNRIMFHQGLADYHGHVSARIPGTRQF
                     FIKPVLAPLGSIAARDIIKVDIDEYKRDCEKNWAKAGNKREVTRLKAPPRETMIHAAI
                     YEARPDVNSVVHTHQPLATAFSVAGTPILPIYNQAAVFAPETPIFPSPRLIYTLQDGK
                     EICQTLGERMAMLLKGHGIVVCGDSLEYATVHAIYLERTAYMQWVASSVGTPAIMPQA
                     DIDYMKENMTYRSYDAFAYFRSLLPKGGRI"
     gene            2623..4227
                     /locus_tag="VFN58_00185"
     CDS             2623..4227
                     /locus_tag="VFN58_00185"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8916432.1"
                     /translation="MAKREARVTESTGKRPIQDLREWLARVEEMGDLIRIKQPVSCDE
                     EMSAIGYLVAKQTPSPAILFESVKGYEKSPFKARLLWNILGPSIPRIALTLEEPSDTP
                     TLELIRRVKDKFRNRMAPREVSPAQAGFFANSVTADKIDLNQLPIPRHWPLDGGRYAG
                     TADVVITRDPDNGYLNVGTYRMMLQGKKEVGLYLSPGKDARLHITRSWQMGKPVEVAA
                     AWGIDPLFMLIGSQTFPKNVSEYEYLGGVKNEPIPVIKGKTTNLLLPANAEFVIEGII
                     KPNSVRKEGPFGEFPGYYGRPEAGCPLVEVTAVHYRDNPILTNALMADYPSCEQSGFF
                     SVIRSARIWDDLDKLGVPGIQGVYAHPAGAGGFGMTVISLEQRYAGHAAQALALAAQV
                     PGGAYYTKWIIAVDEDVDPTDMDQVIWAMSSRCNPIDDIDILRNTWSTWLDPTQNPPE
                     KRPYGSKALINACKEHRYLPVFSKRTALRKEIYDKVAAEWKKLGLPGQVPSVRAFEEE
                     KKVVYHEVGGFEPGKQPGEESSEKKK"
     gene            4729..6441
                     /locus_tag="VFN58_00190"
     CDS             4729..6441
                     /locus_tag="VFN58_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET8916433.1"
                     /translation="MNKLIRALLVEHSDDDAELLVREMRQGGYNIDYLRVETPAALSS
                     ALSEKSWDIILAEYKMPNFNGTDALRLFKRAGLDCPFVIVSGAMSDETGAATMKAGAH
                     DYLLKGNLTRLVPTIERELQQAEIRRQGKVANERLQHLASHDLTTDLPNCAFFSERLA
                     QALLNAQREKNQLAVLIMKVNRFHELNEALGDQLCDVLLMQLGRRLTAELPPSLTFAC
                     LRSNEFAVLIPSAKSSDAALQVVHEITRVLERSFIISEGLKLELQVGFGIALFPEHAT
                     AADLLIRRARAASATAKKSHSQYAFYTLEQHQTAQNRLSLVGDLRRAIVESQLFLLYQ
                     PKVDLASLRLAGVEALVRWKHPQLGILTPDHFIPLAEQTGLIMPLTLWVFNEALRQCN
                     IWNQETVGLDVAVNLSPWNLQSSRLPEQISGLLTSSGVSSSQLELELTESAIMANPKQ
                     AAEILASMKQMGLLIAVDDFGTGYSSLAHLHRLPIDVIKIDKSFVMKMATEAQDAVIV
                     RSMIHLAHNLNLKVVAEGVENRQTIDMLRTMGCDMAQGYYISHPRPAEELPRRLQNWF
                     ESGF"
     gene            complement(6573..6914)
                     /locus_tag="VFN58_00195"
     CDS             complement(6573..6914)
                     /locus_tag="VFN58_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008489817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET8916434.1"
                     /translation="MKKIEAIIKPFKLDEVKQKLTSVGITGMTISEVKGFGRQKGHTE
                     LYRGAEYTVDFLPKVKIEILAPDDMVPKIIEAMLESAQTGKIGDGKIFVSTVEEVIRI
                     RTNERGEEAIS"
     gene            complement(6974..>7612)
                     /locus_tag="VFN58_00200"
     CDS             complement(6974..>7612)
                     /locus_tag="VFN58_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916435.1"
                     /translation="LFQDNGSGMHTHQSLWKGGKNLFYDKKGNGLISDMARYYIGGLI
                     KHAHALCGFIAPTTNSYRRLVPGYEAPINLVYSARNRSACVRIPMYSTSEKAKRLEFR
                     TPDPSCNPYFAFPAMLMAGLDGVRNKIEPPKPIDKDLYELEPQEAAKVKSMPGSLDQA
                     LDALEKDHDFLLRGDVFTKDVIETWLDYKRKKEVDAIRLRPHPHEFALYFDI"
BASE COUNT         1904 a         1979 c         1920 g         1809 t
ORIGIN      
        1 aggatcgatc gctcgaggcc aggaaaatcg acttccgtgg caaccttgtc gaagttaggc
       61 tgcatagtga gaaaagcctg cgtgtgacaa agattaattt ctatcctaag aaagagaaat
      121 tatctagatc ggcatggcgc cgaaaattag ccacgcgggg aaattatatc cgtgggcgat
      181 tgtaggcagc aaatcgaaac catccggctc ttctatcggt ggttgttttt gtgattgttc
      241 aaaccccgga ccattcgaaa ggcaaacata tcggccttgt tttccgcatc cgcccaaaaa
      301 acatagtggc ccatttcatg ataaaccgtg ttaatccact ttcgctctga gttatatttt
      361 ctgccctttt tccagttctc cggatgaacc agatagatct gtccgttttc gtaggtcttg
      421 ccggcggttt tcccccaatc aatgtactca taccagtcta cctgtgggtt cttcagatgg
      481 tagaaacgac agagagccgc tacggccttt ttgaaatcct gaggcttgta ttcatggaaa
      541 aaagctgaaa gcaggcggtc cacgatacga cgttctttca gagatggcag tatcattcgc
      601 atagtacttt tcccagccac gatacccagc atttttacca caaaaaggcc ccaataaaaa
      661 cccagcaaga aacggagcgg gttggcaatt ctaacctaac atattgattt atcggatatt
      721 tttcattgga cggatatttc caaggccaga ctgcgagagc agaattccga tcttgcgacg
      781 gacccaaaag tatcgtagat tggcgtactt tgtgagccaa cgccgttctg tcgaaccgcg
      841 actccagcta cagcccaatg attcaatcat ggaacgaaag agccgcctat ataggcgctt
      901 ggataggttt taacgcgggt tgccttgccg tgagaatttt tccgcgccca tggctttatc
      961 gcttttccga tacactcgct caaattggct tctatctttt tcgaggtttt cgcacacggt
     1021 cgattaaaaa tgttggcttc gcgctgggag agaaattaac tgaaatccag gttgaagaga
     1081 ttgttcgcaa atcgctgcgg aattttttgc gcgactttgt cgagatcgcc atcgcactag
     1141 tggtgcgggc tgaggatttg cgctcggaaa ttcccatcga aggcggtgaa aatctcgacc
     1201 tagctctggc aaaaggacaa ggcgttattg tgttgggcgc gcatctgggg aacttttttc
     1261 ttacgggcac gcgattggcc attgaaaatt accctgctta cattttggtc aatcagccga
     1321 gaaatgggca gtttgccaga ttcatggacg actatcgctt gaaggtccgg caaaaaacga
     1381 ttcatgcccg gcctcgtcag gaggcctggc gcgagcttat tcaagtactg cgacgcaacg
     1441 aactcgccgt cgtgattgct gatgaatatc gtaggaacaa tggaattcat gtgccttttt
     1501 ttggccgcac cgtgctggct cgccggggac cggcgacctt ggccttgagg accggcgctg
     1561 cggttgtgcc ggtctacttg atccatgata agagtcacgg cttgaagatg atcgtcgagc
     1621 ctgaacttga tttgatcaga accgatagag acaaaactgc aatacgcgaa aacacgttgc
     1681 gtatgacgca atggctggaa agaaccgtgc gagcctatcc ggagcagtgg aattggatga
     1741 acattcagtg gcgagaagat agggacaccg cacccgtcgc caaagaacgg cgagttcaag
     1801 gctttagttc ttaggataga aaggggacca aaagtggcaa gcagcaagag ggaactggac
     1861 gaactcaaaa agaaagtcgc cttgggtaac cgcatcatgt tccatcaggg tctggcggac
     1921 taccacggcc acgtgagcgc gcgaattccg ggcaccaggc aattttttat caagcccgtc
     1981 ttggcgccgc tgggttcgat tgcagccaga gatatcatca aagtcgatat cgacgagtac
     2041 aagcgggact gcgagaagaa ttgggcgaag gccggtaaca aacgagaagt tacccggtta
     2101 aaagcgccgc cgcgagaaac gatgatccat gctgccatct atgaagcacg accggatgtc
     2161 aactcggtgg ttcacacgca tcagcctttg gcgacagcat tttccgttgc cggcacaccg
     2221 atattgccta tttataacca agcggctgtc ttcgcgcctg agacgccgat tttccccagt
     2281 cccagactca tctacacgct tcaagatggc aaggagatct gccaaacctt gggtgagcgc
     2341 atggcgatgt tacttaaggg tcacggcatt gttgtatgcg gcgatagctt ggaatatgct
     2401 acggttcacg ctatctacct ggaacgaacc gcctacatgc aatgggtggc gagctcagtg
     2461 ggcacgccag caatcatgcc ccaagccgac attgattaca tgaaagagaa catgacgtat
     2521 cgttcctacg acgcctttgc ctacttccgg tccctattac ccaaaggcgg gcgaatataa
     2581 gattcgaatt cccgacactg catccatttg gaggttatac cgatggctaa acgagaagcg
     2641 agagtaacgg aatccacagg caagagaccg attcaagatc tgcgtgaatg gctcgcgcgc
     2701 gttgaagaga tgggcgatct tattcggata aagcaacccg taagctgtga cgaagagatg
     2761 agcgcgattg gttacctcgt cgccaagcaa actccctctc cagcgatcct gtttgaatcc
     2821 gtcaaaggct atgaaaagag ccccttcaag gctcgactac tatggaacat cctaggtccg
     2881 agcatccccc ggattgcgct cacccttgaa gagccttccg acacaccaac tctcgagctc
     2941 atccgccgtg tcaaagacaa gttcagaaat cggatggcgc cgcgtgaagt atcgcctgcc
     3001 caagccggct tctttgccaa cagcgtcacc gccgacaaga tcgacttgaa tcagttgcca
     3061 attccgagac attggccttt ggatggtggt cgctatgctg ggacggccga tgtggtcatc
     3121 acgcgggacc cggacaacgg ttatttgaac gtcggtactt accgcatgat gcttcaaggt
     3181 aagaaagagg tcggcctcta cctctcaccc ggcaaagacg ctcgtttaca catcacccgt
     3241 tcctggcaga tgggaaagcc tgtggaagtg gcggcggctt ggggtatcga tccgctattt
     3301 atgctcatcg gctcgcagac ttttccaaaa aatgtctctg aatatgaata tctcggaggg
     3361 gtcaagaacg aacccatccc tgtcataaaa gggaaaacga cgaatctgtt gctgcccgcc
     3421 aacgcagaat ttgtcattga gggaattatc aaaccgaact cggtcagaaa agaaggaccc
     3481 ttcggagagt tccccggata ttacggcagg ccagaagcag gttgtccgct cgttgaagtg
     3541 accgctgttc attatcgaga caatccgatt ctaacgaatg cactcatggc ggattacccc
     3601 tcctgtgaac agagtggttt cttctccgtg attcgttccg cccgtatctg ggatgatcta
     3661 gacaagctcg gcgtgcctgg aatccaagga gtttatgccc accccgccgg cgccggggga
     3721 tttggcatga cggtcatcag tttggagcaa cgctacgccg gccatgcggc gcaggcattg
     3781 gctcttgcag cccaggtacc cggtggcgcg tattacacga aatggatcat tgccgtcgat
     3841 gaagacgttg accccacgga tatggatcag gtcatctggg cgatgagcag ccgttgcaat
     3901 cccatcgacg acattgacat cctacgcaat acctggagca cctggctcga ccccacgcag
     3961 aacccgccgg aaaaacggcc ttacggttca aaggcgctga tcaacgcctg caaagaacat
     4021 cgttatctgc ctgtgttctc taaacgcact gccttgagaa aagagattta cgacaaggta
     4081 gcggctgaat ggaagaaact cggcttgccg ggacaagttc caagtgtaag agcgttcgaa
     4141 gaggagaaga aagtcgtcta ccacgaagtg ggcggcttcg agccgggaaa acagcctggg
     4201 gaagaatcgt cagagaagaa aaagtagctc caattcactt accgatctaa attcgttagc
     4261 gattgacctg gaacccacgt ttcaatccag gtcaatcgtt ctgtcaagaa aaccccagat
     4321 cgtaacgtaa tggccacttt tgtccagact ttgtcaagct tggactgttg gtcacttttc
     4381 tgtcgtccca aatataacaa gaattaacaa gcaaatcggt gaattatgct cgtccaaaaa
     4441 aatggcatga gacctgcttt ttctcagtgt ttcaatttgg gtttgtaccg agacagatca
     4501 aagccggcag ggtacgtgcg tcgagcgagc caccatgcct acaattaggt tgagttaaca
     4561 ggattccaca gtggcagctc tccggccaca agcgcaccct ccacgaatag agcctcaggt
     4621 ccggatccat ctaaattcag aatagcgtag agccaggact tttcggaagc atcttgctaa
     4681 ccgaagtcgt cgaagggtct atagccgctg gcaacaaagg atattcaatt gaataaattg
     4741 attcgcgcgt tgttagtaga acattcggat gacgacgctg aactgctagt tcgagaaatg
     4801 cgtcaaggtg gctacaacat agactatcta cgggtcgaaa cacccgctgc gttatctagc
     4861 gcgctgtcgg agaaaagctg ggatatcatc cttgccgagt acaaaatgcc caactttaat
     4921 ggcaccgacg ctctgagact attcaagcgc gcaggtctcg attgcccatt cgtcatcgtg
     4981 tctggagcta tgtccgacga gaccggcgca gcgaccatga aggcaggtgc tcatgactat
     5041 ctcctgaaag gaaatctcac gcgtttagtt cctaccatcg agagggagtt acaacaggca
     5101 gagatacggc gccagggcaa agtagcgaac gagaggttgc aacatctcgc ttcccatgat
     5161 ttgaccactg acctcccaaa ctgcgcattt ttttccgagc gtctcgctca ggcgctcctc
     5221 aacgcacagc gcgagaaaaa tcaacttgct gtcttgatca tgaaagtcaa ccgatttcac
     5281 gagttgaatg aagctctagg cgatcagtta tgcgatgtcc tattgatgca attggggcga
     5341 cggctgaccg cggagttgcc tccgtcgctc acatttgcct gtctgagaag caatgaattc
     5401 gccgttctga ttccctctgc taaaagctct gatgccgcgc tgcaagttgt ccatgagatt
     5461 actagggttt tggaacgatc atttatcatt tcagaggggt taaaattaga gctccaggtc
     5521 ggatttggaa tcgcactctt tcccgaacac gcgacggccg ctgacttact catccgaagg
     5581 gctcgcgcgg catccgccac cgcgaaaaag agccatagcc agtatgcctt ttacacattg
     5641 gaacagcacc aaactgcgca aaatcgactt tctctggttg gagatctacg tcgagcgatc
     5701 gttgaaagcc agttgttctt gctctatcaa cctaaagtgg atcttgcttc gctccggttg
     5761 gccggtgttg aagcactcgt gcgctggaag cacccccagc tcggcattct cacgcctgac
     5821 cacttcatcc ctttggctga gcaaacaggt ctcattatgc cgctcacttt gtgggttttt
     5881 aatgaggcgc ttcgccaatg caatatttgg aatcaggaaa cggttggcct cgatgtcgcg
     5941 gtcaatctct cgccttggaa cctgcaatcg agccggctac cggagcagat cagcgggctc
     6001 cttacctcgt ctggagtatc gtcttctcag cttgagctgg agcttaccga aagcgcgata
     6061 atggcaaacc ccaagcaggc cgctgaaatt ctggcaagca tgaagcaaat gggattactg
     6121 attgctgttg atgattttgg aaccggttat tcttctctgg ctcacttgca cagattgccc
     6181 attgatgtaa tcaaaattga taaatccttt gtcatgaaaa tggcaaccga agcccaagac
     6241 gctgtgatcg tgcgatccat gatacatctg gcccacaacc ttaatctgaa agtcgtcgct
     6301 gaaggagtag agaaccggca gactatcgat atgctacgca cgatgggttg tgacatggcg
     6361 cagggttatt acatcagcca cccgcgcccg gcagaggaat tgccacgaag actgcagaat
     6421 tggttcgaga gcggatttta gacggcctac ggaacacaag ttgcctatac ccttcgcgag
     6481 tgaacctgga aaaatcacct gcagcaagac gaccggttgt aaactatcgc agattcagat
     6541 ctccctgatt ctcgatcgat ggaatagaaa ctctaactga tggcttcttc gcctctctcg
     6601 ttggttcgta tacggatcac ttcctctacc gtcgacacga aaatcttccc gtccccgatt
     6661 tttccggttt gcgccgactc gagcatcgcc tcaatgatct tgggtaccat gtcatcgggc
     6721 gccaagattt caatcttcac tttgggaaga aaatctacgg tgtattcggc cccacgataa
     6781 agttccgtgt gccctttctg ccttccgaag ccctttacct cgctgatggt catcccggta
     6841 atgccaacgg atgtgagttt ctgttttacc tcatccagtt tgaaaggctt gataatcgct
     6901 tcgattttct tcatagaatc ctccttccca cgcgtctaaa gagaaagata accgcctcta
     6961 gcccataccg cttttagata tcgaagtaca gggcgaattc atggggatgg ggtctgagcc
     7021 ggatcgcatc gacctccttc ttgcgcttgt agtccagcca ggtctcgatc acatccttgg
     7081 taaacacatc gccgcgaagc agaaagtcgt gatccttctc caacgcatcg agcgcttgat
     7141 ccagcgaccc cggcatcgac ttcactttgg ccgcctcctg cggctccaac tcatataagt
     7201 ccttatcaat cggcttgggc ggctcaatct tgtttcgaac cccgtccagt cccgccatca
     7261 acatcgccgg aaaagcaaaa taggggttac agctcggatc gggagtacgg aattcgagcc
     7321 gcttggcctt ctcactggtc gaatacatcg gaattctaac gcaggcgctt cggttacggg
     7381 ccgagtacac tagattgatc ggcgcctcgt agcccggaac cagccttcga tacgagttgg
     7441 tcgtcggcgc tataaagccg caaagcgcat gggcatgctt gattaagccc ccaatataat
     7501 accgcgccat gtcactgatc agcccgttgc ccttcttgtc gtaaaacagg ttcttgccac
     7561 ccttccacag gctctggtga gtgtgcatcc cggagccatt gtcttgaaat ag
//
LOCUS       DASSWZ010000010         4284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_481433, whole genome shotgun sequence.
ACCESSION   DASSWZ010000010 DASSWZ010000000
VERSION     DASSWZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481433"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFN58_00205"
     CDS             <1..222
                     /locus_tag="VFN58_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916436.1"
                     /translation="SLDAGKSWKKLDGEVSRANQSFVEAFAFDPVNFDRMYAAQQNGD
                     IFGSENSGASWFKLNVKAPHVSDMKAARA"
     gene            293..1195
                     /locus_tag="VFN58_00210"
     CDS             293..1195
                     /locus_tag="VFN58_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916437.1"
                     /translation="MAHLVGCVAMSHGPQLMLNPDQWDLLKNRESEGLPDKPGLENET
                     AEVKWTKWRGCMDAIATLRQKLNELRPDVVIVVGDDQHENLVDDNMPPFTIYMGETAE
                     ASVSLRYLKQPKSENRTRYQVHFKMAEELINGLMEEGFDPAYSKQTRYDGGLGHAFAR
                     VLKFLTPDAQCRVVPVMVNTYYPPAPSAKRCVKFGQTMASILRRFPGQERVVMVGSGG
                     LSHTKISEPLDYGFLKALETNDLEYMGAMPAADLVEGTSEIRNWIVTAGAANRPGKVV
                     KYFPLYRTRTGVGCAMGFAQWELN"
     gene            1256..2554
                     /locus_tag="VFN58_00215"
     CDS             1256..2554
                     /locus_tag="VFN58_00215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8916438.1"
                     /translation="MTDAPAPSDPKEIGRLAAFHYRDFRLFWISLFVSNIGTWMQMTA
                     TNWLLYQLTDSPVQLGLNGIFRALPAIGFGLISGTLADRYDRRRLMLWTQLLLGLLAL
                     GLGVLDHSGHVQAWQIYAFTFVSSTVGSFDGPARQALFPSLVPRAVLPNAVALNSLLW
                     KGAALLGPTLGGVAISLMGTAGAFYANAASFLVVVVALLLMRTSASARRRHHDFLSET
                     KAGFSYIVSRPIVLGVMIMEGVSSVFGLDNAMLTIFASDILKVGASGFGLLQSARGLG
                     AVIGSSLFIAMGHRSDHGKILFTSAVLYGIGFALFGLSPSFLLSLALIAFVGVTDTIW
                     SACRSTILQLITPENFRGRVMGVFQLSNRGLHPLGQTETGLVVPLMGAPAATFFGGLL
                     VTVVTLITAWRVPEILKFRWNETEDHRTDRSVADDERATG"
     gene            complement(2621..3736)
                     /locus_tag="VFN58_00220"
     CDS             complement(2621..3736)
                     /locus_tag="VFN58_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916439.1"
                     /translation="MSFLRIQDLYKTFDRVVAVDRLNLEVREGEFFTLLGSSGCGKTT
                     TLRLVGGLEKPDGGEIHLGDKCLASERQKLFVKPEKRDMGMVFQSYALWPHMTVFENV
                     VYPLKLRGIRGREAEKKVAEVLELVGLGGLEERPAPALSGGQQQRVALARALVFSPKV
                     LLLDEPLSNLDAQLREEMRRELKALQRRVHVTVVFVTHDQIEALSLSDRIAIMKTGQL
                     EQVGSPEEVYYRPATPFVRDFLGKTFLLPGKILGIADQQVNVAIQQFDASPLAIQRTN
                     LSASGNGLTGVGQSVMVAIRPEQIELWAKPPEGKLNMVPANLQSVQFLGDRYEYTVAL
                     GSETRVLVSPAARHLRAGEKIYLELKSEGITLWPREV"
     gene            complement(3882..>4284)
                     /locus_tag="VFN58_00225"
     CDS             complement(3882..>4284)
                     /locus_tag="VFN58_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8916440.1"
                     /translation="IVLLVIALVLGGITLSTQILKANFVQLGNELEEASRMSGAGFWR
                     TYFQVVFPLMAQTMVLVAVLKFTFASQNASSIILLATSETRTLSLLALDQIAAGYREV
                     ASITVIYITLLTLGVALIARSFGLKVGIRAD"
BASE COUNT          939 a         1218 c         1157 g          970 t
ORIGIN      
        1 tccttagatg ccggcaagag ctggaaaaaa cttgacggcg aagtttcaag agcgaaccag
       61 agcttcgtgg aagcctttgc ttttgacccg gttaacttcg accgaatgta cgcggcgcag
      121 caaaacggcg atatcttcgg gagcgaaaac agcggcgcca gttggttcaa attgaacgta
      181 aaagcgccgc atgtctccga catgaaggcc gctcgcgcgt aagtttaggg cccgtcgtaa
      241 accaaagact aaacgttgaa cttggaacct tgaaatcgga accgaatacc ttatggctca
      301 tttagtcggc tgtgtggcca tgtcccacgg gccacaattg atgctcaatc ccgatcagtg
      361 ggacctgctc aagaatcgcg agagcgaggg attgccggat aaacccggct tagaaaatga
      421 aactgcggaa gtgaaatgga cgaagtggcg cggctgcatg gacgccatcg caacgcttcg
      481 acaaaaattg aacgagctcc gcccggatgt cgtgatcgta gtgggcgatg atcagcatga
      541 aaacttagtc gatgacaaca tgccgccctt cacgatctac atgggggaaa cagccgaggc
      601 gagcgtcagc ttgagatacc taaagcagcc gaagtctgag aatcgaaccc ggtatcaggt
      661 tcatttcaaa atggccgaag agctcatcaa cggcttgatg gaagaagggt tcgatcccgc
      721 ctattccaaa caaacgcgct acgacggcgg gctcggccat gccttcgcgc gcgtgctcaa
      781 atttcttaca ccggatgccc agtgtcgcgt ggttcctgtg atggtgaata cctattatcc
      841 cccagcgcct tcggccaagc gttgtgtaaa attcggccag actatggctt cgattctgcg
      901 ccggttcccc ggccaggagc gagtcgttat ggtcggctcc ggagggctca gccatacaaa
      961 gatcagcgag cccctggatt acggctttct caaggcgttg gagaccaatg atctcgaata
     1021 catgggagcc atgccggctg ccgatttggt cgagggcaca tccgagatcc gcaactggat
     1081 tgtgactgcg ggtgcggcga ataggcccgg caaagtcgtc aaatactttc cgctgtaccg
     1141 gacccgcacc ggtgtcggtt gtgccatggg ttttgcgcag tgggagctca actagtcgcc
     1201 attcccgtcg caatccaaaa catcggccgt acatcacgtt taaagtcccc atcgaatgac
     1261 cgacgcacca gccccctcgg acccgaaaga aatcgggcgt cttgctgctt ttcactatcg
     1321 cgatttcaga cttttttgga tcagcctttt tgtttcgaac atcggcacct ggatgcagat
     1381 gactgcaacc aattggctgc tctatcaact gacagactct ccggttcagc tgggactcaa
     1441 cggtatcttc cgcgcgctcc ctgccatcgg cttcggtctc atcagcggca cgttggccga
     1501 tcgctacgat cgccgccgac tcatgctctg gacccagttg cttttaggtt tgttagcgct
     1561 aggtctcggc gtattggatc attctggtca cgttcaagcc tggcagatct acgcgtttac
     1621 cttcgtcagt tccaccgttg gctccttcga tggtccggcg cgtcaggctt tgtttccttc
     1681 gctggtgcca cgcgccgtcc tccccaatgc ggtggcgctc aattcattgt tatggaaagg
     1741 agcggcgctc ctcgggccga ctctgggtgg cgtcgccatc agcctcatgg gcaccgccgg
     1801 ggcgttctac gcaaatgccg caagtttcct ggttgtcgtc gtcgcgctct tgctcatgcg
     1861 aacttcggca tcggcgcgcc gaagacatca tgatttcttg agtgaaacga aagcggggtt
     1921 ttcctatatc gtctcgcgcc ccatcgttct gggtgtgatg atcatggaag gggtctcgtc
     1981 cgtcttcggt ttggataacg cgatgctgac catcttcgcc agcgatattt taaaagtagg
     2041 tgccagcggg ttcggactct tgcagtcggc ccgtggtctc ggcgccgtca tcggctcgag
     2101 tctgttcatc gccatgggcc atcgctcgga tcatgggaaa atacttttta cttcggcggt
     2161 tctctacggg atcggctttg ctctgttcgg tctttcccct tcgttcctgc tctctttagc
     2221 gctgatcgca ttcgtcggcg tgacggacac gatctggtcc gcttgccgca gcacgatcct
     2281 tcaactaatt acgccggaaa atttccgcgg ccgtgtgatg ggagtttttc agttgagcaa
     2341 ccgggggctt catcctttgg gtcagacgga gaccggattg gtcgtgccgc tgatgggggc
     2401 gccggcagca accttttttg gcgggctgct ggtaaccgta gtaaccttga tcaccgcttg
     2461 gcgggtgccc gaaatactga aattccgctg gaacgaaacg gaagatcacc ggactgaccg
     2521 cagtgtggcg gatgacgaac gagcaaccgg ttaaaccggc ttgcgggatt ttcagatcac
     2581 aaagttaaga tttgagattt cgagcgccag cgaggacgct tcacacctct ctcggccaga
     2641 gggtgatacc ttccgacttg agctccagat agatcttttc accggctctt agatgtcgtg
     2701 cggcgggtga aaccaacact cgagtttccg agcccaacgc caccgtgtat tcataacggt
     2761 cgccgagaaa ttggactgac tgaagattcg ccggcaccat gtttagctta ccttcaggtg
     2821 gcttggccca taattcgatc tgctcgggtc ggatcgcaac catcacggac tggccgactc
     2881 cggttagacc gttgccggaa gccgaaagat ttgttctttg aatggcaagc ggagaagcgt
     2941 caaactgctg aatcgccaca ttgacttgct gatcagcaat cccgagaatc ttacccggaa
     3001 ggagaaaggt cttgcccaag aagtcccgca caaacggcgt cgccggacga tagtagacct
     3061 cttcaggact gccgacctgt tccagttggc ccgtcttcat aatggcgatg cgatcggaga
     3121 ggctcagagc ttcaatctgg tcgtgggtga cgaatacgac ggtgacgtgg acccgccgtt
     3181 ggagcgcttt caattctcgc cgcatctcct cgcgcagttg cgcgtccagg ttgctgagcg
     3241 gctcgtcgag cagcaaaact ttcggcgaga aaaccaacgc gcgtgccagc gcaacccgtt
     3301 gctgctgccc gccgctgagc gccggcgcgg gacgctcctc tagacccccg agccccacca
     3361 actccaagac ctcggcgact tttttttcgg cctctctgcc tctaataccg cgcaacttca
     3421 aaggatagac aacgttttcg aaaaccgtca tgtgcggcca tagggcgtag gattgaaaga
     3481 ccatgcccat gtcgcgtttc tccggcttga cgaatagctt ctgcctttca gaggcaaggc
     3541 atttgtctcc aagatgaatc tctccgccgt cgggcttctc caatcctccc accagccgga
     3601 gggtcgtggt tttgccgcaa ccgctcgatc ccaacaaggt aaaaaattct ccctcgcgaa
     3661 cctcgagatt cagccggtcg accgccacca cccgatcaaa ggtcttgtag agatcttgaa
     3721 tgcgcaagaa actcactggt cttgtcctcg gcagcacgcg tgaagcgata caattgcaga
     3781 tgatgatcga gcagcgatga tagaggatcg cgatcttcga tcctccatct tcgaccctcg
     3841 accctccatc ctcgatcctc tatcctcgac cttctttaat gtcagtcggc ccggataccg
     3901 acctttagtc cgaaagacct ggcgatcaaa gccacaccca aggtcagaag cgtgatatag
     3961 atgacggtaa tactggcaac ctcgcgatag cccgccgcaa tttgatctaa agctagaagc
     4021 gatagcgtcc gggtttccga agtcgcaagt agaatgatgc tcgaagcatt ctgcgaagca
     4081 aatgtgaact taagcaccgc caccaatacc atggtctgcg ccataagggg aaagaccact
     4141 tgaaagtagg tgcgccaaaa accggcgccc gacatgcgcg acgcctcttc cagttcatta
     4201 cccaactgga caaagttggc cttgaggatt tgggtcgaca acgtaatgcc acccaagacc
     4261 aaagcgatca cgagcagaac gata
//
LOCUS       DASSWZ010000011         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1033327, whole genome shotgun sequence.
ACCESSION   DASSWZ010000011 DASSWZ010000000
VERSION     DASSWZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033327"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFN58_00230"
     CDS             <1..539
                     /locus_tag="VFN58_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916441.1"
                     /translation="KGLLHPRDFSMVVISGGTSARLTALESGAIAGGVLGIPYSDIAV
                     DQGFNRLGDTLELIPTYQFNAVTINPAWAEKNRGTVVRFLKAHIRSLRWIYEQPDQAA
                     EFFTQEMGVKPPYARRGVDYFTKNKIFPIDGAVTLDGLKINIEVQARDGVLKEPLPSP
                     EKYVDLSYLRQAQKELGL"
     gene            704..>2161
                     /locus_tag="VFN58_00235"
     CDS             704..>2161
                     /locus_tag="VFN58_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916442.1"
                     /translation="MIPLLQRWKEIHRSFTHTPQAVKLVWQTNRSATLGLGFLTLAGA
                     LLPASQAWVGKLIVDGVVASIQGGADPEVVRTVFVYLILELALFLLNTALNQARRLIQ
                     QLIQLQLANRIRGKIIQKALTLDLAFFEHPDFYDRLQNARRESGYKPVDLINDTFLIV
                     QNTITLISFAVLLLRFSPWLVIVLLVTSIPAFIAETRFSEEGFRLLTRRAPETRQINY
                     LSRLLTEDTSAKEIKLFNLGETLLTRYMTLFDKFFREDKSLALRRAVAGFGLGLIATL
                     GFYGSYAWIVWHTVQGKISLGDMTLYLTIFRQGQTTFQGILSGIGNIYENNLFMANLF
                     DFLGLKPQMGVAEQNQGLSVPLRSGIEFRGVGFRYPDSEEWALRDIDLTIRPGEKIAL
                     VGPNGAGKTTLIKLLSRLYDPTEGTILIDGIDIRALDPLDLRQRIGVIFQDFVRYHLA
                     ASENIGFGQIEALDRMDQIIASARKSGAHSVIENLP"
BASE COUNT          467 a          612 c          557 g          525 t
ORIGIN      
        1 gcaaagggct tcttcatccc cgcgatttct ccatggtggt catctcgggc ggaacgtcgg
       61 cgcgcttgac cgcgcttgaa agcggcgcga ttgcgggtgg ggttctaggc atcccatatt
      121 ccgacattgc cgttgatcaa ggattcaacc ggctaggtga cacgctggaa ttgatcccca
      181 cgtatcaatt taacgccgtt actatcaatc ccgcttgggc agagaagaac cgtggcaccg
      241 tggtcagatt cctcaaagcc cacatccgca gcctgcgctg gatttacgag cagcccgacc
      301 aggccgccga gttcttcaca caagagatgg gggtcaagcc gccctatgcc cgcagaggtg
      361 tcgattattt caccaagaac aaaatcttcc cgatcgacgg cgccgttacc ttagacggct
      421 tgaagataaa tatcgaagtg caggcccgag acggggttct taaagagcct ttgccttccc
      481 cggaaaaata tgtggatctc agttacctca ggcaagcgca aaaggaattg gggttgtagc
      541 gtgcctgggg aatgacgagc cccttctaaa cttgactgga ttagcgccct ttcgaaatgc
      601 tcgccgtgtt attctgaata ccgtcgcccg ttctacgttg cgctcgtacc cgatcggtgg
      661 ccaactctct gacgatccga ttttcgttta ggaggcattt tctatgatcc ccttgttgca
      721 aaggtggaag gaaatccatc gttctttcac ccacactccc caggctgtca agctcgtgtg
      781 gcaaaccaac cgctcggcca ccctaggttt gggttttctc accctggccg gcgccctctt
      841 gccagcgtct caggcatggg tggggaagct catcgtggat ggagtcgtag cctctatcca
      901 ggggggcgcg gacccggaag tggttaggac ggtttttgtc tacttgatcc tcgaattggc
      961 tcttttcctg ctcaacactg cgctcaatca ggcgcgccgg ctcatccaac aactgatcca
     1021 acttcaactt gccaaccgca ttcgcggaaa gattatccag aaagcattga ccttggatct
     1081 cgctttcttc gaacatcctg atttttacga ccgcttacag aacgcacgcc gcgaatcggg
     1141 ctacaagccc gtcgatctga tcaatgatac tttcctgatc gtgcaaaaca cgatcacctt
     1201 gatctcgttt gccgtgctgc tgctgcgctt tagtccttgg ctcgtcattg ttctcttagt
     1261 cacaagcatt cccgctttca ttgccgagac acgcttctcg gaagagggtt tcagactgct
     1321 cacccgccgg gcgcccgaaa cccgacagat caactacctg tcccgtctat tgacggaaga
     1381 cacctcggcg aaggagatca agctcttcaa cctcggcgag actttactga cgcgctatat
     1441 gaccctgttc gacaagttct ttcgcgaaga caaatcgctg gcgctaaggc gagccgtcgc
     1501 gggattcggc ctgggtctga tcgccacctt gggattttac ggctcctatg cgtggatcgt
     1561 ctggcacacc gtccagggga aaatctcact cggcgatatg accctttatc tgactatttt
     1621 tcgccagggc caaaccacct ttcaaggtat tttgtcgggc atcggcaaca tttatgagaa
     1681 caatctgttc atggccaacc ttttcgattt tctagggttg aaacctcaga tgggcgttgc
     1741 cgagcaaaat caaggattat ctgttcccct tcgcagcggt atcgaatttc gcggcgtcgg
     1801 gtttcgctat ccggatagcg aagagtgggc gttgcgtgac atcgacctga caatccgccc
     1861 cggcgagaag atcgcgttag tggggcccaa cggcgccggc aagacgactt taattaaact
     1921 tttaagccgc ctttacgatc caaccgaagg cacaatattg atcgatggca tcgatattag
     1981 ggcgttggac ccgctcgacc tgcggcaaag aatcggcgtc atctttcagg acttcgtgcg
     2041 ctatcacttg gccgccagtg agaacatcgg ctttggccag atcgaagcgt tggacaggat
     2101 ggatcaaatc atcgcatccg cccggaaaag cggtgctcat tcggtgatcg agaatcttcc
     2161 g
//
LOCUS       DASSWZ010000012         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_170105, whole genome shotgun sequence.
ACCESSION   DASSWZ010000012 DASSWZ010000000
VERSION     DASSWZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170105"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFN58_00240"
     CDS             <1..615
                     /locus_tag="VFN58_00240"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8916443.1"
                     /translation="SPKPILKIHAITHRRDPILPFSNMGMPVHEGQTATALIKGAEIY
                     GELKKLGLPVKGVFCPPYGVGHMAVVSTDVPFVNFARRVAHAVWATKPGLFTYYIVIV
                     DADVDPTNMDEVLHAMTTKCHPVNGIHQVPHNPGFPVLLPFLPQKERLVGDAAGVIFD
                     CTWPKDWPQESIPIKASFENLWPKEVQERVLQNWQAYGFTTPNP"
     gene            complement(829..1038)
                     /locus_tag="VFN58_00245"
     CDS             complement(829..1038)
                     /locus_tag="VFN58_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8916444.1"
                     /translation="MSIKKTDTAIRLLTLAEAASILKISKRTLHRMIQQKQIPAFKVG
                     GQWRILESRFQEWVQEEESSSPKLN"
     gene            complement(1279..1365)
                     /locus_tag="VFN58_00250"
     tRNA            complement(1279..1365)
                     /locus_tag="VFN58_00250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1329..1331),aa:Leu,seq:gag)
     gene            complement(1383..1772)
                     /gene="secG"
                     /locus_tag="VFN58_00255"
     CDS             complement(1383..1772)
                     /gene="secG"
                     /locus_tag="VFN58_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET8916445.1"
                     /translation="MVIAITIIHVIVSIGLILVVLLQTGKADMGAVFGGSSSTIFGSS
                     GAGNFLTKLTTGMAIVFMITSLTLGYFAGHKPSVTIFDSRTPTPQTMPAAPETPPVPK
                     QSDATGSAPTQTAPNSPQATQPQKSTK"
     gene            complement(1953..3131)
                     /locus_tag="VFN58_00260"
     CDS             complement(1953..3131)
                     /locus_tag="VFN58_00260"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET8916446.1"
                     /translation="MIRRVEDLNLKDKTVFLRVDFNVPIEQGRIIETHRIESALPTIL
                     LILNRAKKLLVASHLGRPDGKVVPNYSLRPIRDHLEQSLKQPVLLAPDSIGPEVERMV
                     QDPSQRLILLENLRFHKEEESNDMAFSKALASLADVYVDDAFGAAHRAHASISGMAAF
                     FKQKGAGLLLHKELEYLSRLLLNPEKPFVTILGGAKVSDKIGVIRNLLPKVNTLLIGG
                     GMAYTFLKAKGIDIGKSLVETDRVPLAKELLKQAAEQGVSTFLPVDHIIGDSERKNPT
                     ICTGNIPPDQMGLDIGPQTITQFSQELQHAGMVLWNGPVGLFEMEPYSKGSQALARKL
                     AEGYPRLVSVIAGGDTVAAVTAAGVAARITHLSTGGGATLEFLEGKELPGIKALEETP
                     "
     gene            3247..3987
                     /gene="tolQ"
                     /locus_tag="VFN58_00265"
     CDS             3247..3987
                     /gene="tolQ"
                     /locus_tag="VFN58_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HET8916447.1"
                     /translation="MTSLWLLRLLAQDGLAASPQHGILDLVRGSGPVVQFILYLLILF
                     SVASWGIIVYKFRQLRRAKRESEKFIDVFWERRNLSSIHEASRDLKACPVAQVFRSGY
                     EELVRVSRSKKENPSGEALTTELGGVENVSRAMKRATSVEITKLEKYLTFLATTASAA
                     PFIGLFGTVWGIMNAFRGLSVTHSSSIQAVAPGIAEALIATAVGLAAAIPALMAYNYF
                     VQQTKVLAAEMDNFSHEFLNIAERHFFK"
     gene            4003..4425
                     /gene="tolR"
                     /locus_tag="VFN58_00270"
     CDS             4003..4425
                     /gene="tolR"
                     /locus_tag="VFN58_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HET8916448.1"
                     /translation="MAMDGASQRDATTIAQINVTPLVDVMLVLLVIFMVTAPIIQQGV
                     QVNLPQAKAGAIPGTEELLVVTIAKNGKVYLNDNPMTLGELGDKLRAIRKLQADKQVY
                     LRADQDVRYGLVMKAVAEIKQAGIERLGMVTRPSSDEK"
     gene            4430..5353
                     /locus_tag="VFN58_00275"
     CDS             4430..5353
                     /locus_tag="VFN58_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET8916449.1"
                     /translation="MAGHQSTQTISSQERSLPLVDREDKLSKWLFFSFLFHGALILAL
                     FVVPFLPARSRPSYPVYTVDLVGGEKIGRNNLGTELVPPSAPKAAPKKTAEEVPPPPA
                     QETKQETKKEKPKPIEKEAPAEEKVALKEATKKEPPKKEPAKDTKSESKTDESALDRV
                     RERLIQSAVDRAKSRTEGAQNPSKGDVISSGQGEGEGAAALGPGGRGGGVVKGMEFII
                     YQNRMLSSIKDNWAWVGQKSNLKVVVHFSIKDSGEITGLKIVQSSGDQSYDESVLRAV
                     KKSSPLPPPPESSRKDFADVELTFRPKDLGA"
     gene            5358..6683
                     /gene="tolB"
                     /locus_tag="VFN58_00280"
     CDS             5358..6683
                     /gene="tolB"
                     /locus_tag="VFN58_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET8916450.1"
                     /translation="MIVKTIFAVLLMILNPWPANGQPKGDTGVRGEIVGQGGLKFPIA
                     VSPLKNLGTTADVGKLSEGIADTIVYDLDLSGWFKVLDRAAYIENPQKSGVALGSFDF
                     KDWSTIGAEGLVKGGFTIQGDEVTVELRLFDVFQNKERVGKRYVGRVKDYRRIAHKFA
                     DEIINQFTGVPGIFNTRITYVSTSGGRFKEIHIAHLDGSEKFQVTNNHTINLSPSWTA
                     DGKSVLYTSYKDRNSSLYLFELYSGKEAKITPRNGRYLGGKASPDGQFIAATVETGGN
                     SNIVLLDRNGNMIRSLTESPGIEVSPAWSPDSKQLVFVSDRSGSPQVYVLDVASNKSR
                     RLTYSGNYNTSPEWSPRGDRVAYTGRVGNRFAIFTIGVDGGESRKLTAESADSEDPTW
                     SPDGRFIAFSSNRAGKYHLYVMQANGENQRRLTGSGGDDTKPSWSPRLE"
BASE COUNT         1657 a         1632 c         1825 g         1675 t
ORIGIN      
        1 tcaccgaagc cgattttgaa aatacacgcg attactcatc ggagggatcc gattctgccc
       61 ttctcgaata tggggatgcc agtacatgag ggacagaccg caacggcttt gatcaaaggc
      121 gcagagatct acggggaatt gaaaaagctc gggctgcccg tcaaaggggt cttctgcccg
      181 ccttacggcg tcggtcacat ggcggtcgtt tctactgatg ttccctttgt gaattttgcg
      241 cgccgagtcg ctcacgccgt gtgggcaacg aaaccaggcc tgttcacata ttatattgtt
      301 attgtggatg ccgatgtgga tcctactaac atggatgaag tgctccacgc gatgacaacc
      361 aaatgtcatc ccgtaaacgg cattcaccaa gtcccacaca atcctgggtt cccggttctt
      421 cttccgtttc taccccagaa agaaaggctc gttggggacg cggcgggagt gatctttgac
      481 tgcacgtggc caaaggattg gccccaagaa tcaatcccga ttaaggcgag ctttgagaat
      541 ctctggccca aagaggtgca agagcgggtg ctgcaaaatt ggcaggccta tggatttaca
      601 actcctaacc catagcttca tacgctggcc gccaaaggag taaattatac ggacagtagc
      661 aactgacgag tggttctcat agcacatata gtagcttcag ctgatactcc ggggttgtgt
      721 actccggagt gatgcggaaa gaattttttc ttggactgaa gattcgaaac tggaccttgg
      781 tgagctgaaa cgtgaaagga atttggaatc gctgttttta ctggggagct agttaagctt
      841 gggcgacgag ctttcttctt cttggaccca ttcctgaaac cggctttcga ggatgcgcca
      901 ttggccgccg actttgaagg ccggaatctg cttctgctgg atcattctat gcaaagtacg
      961 tttcgaaatc tttaagatac tcgcagcttc cgcaagcgtt aataacctaa tcgctgtatc
     1021 ggtctttttt atagacatgt cgcactcctg aaccctcgca gattcagcaa gccttatgcc
     1081 atgggccagt ggcatcgtaa taggttgttt ttgaaggata atcggagcct ttcgaggata
     1141 tggttttcct ccacctagag gaaaaatgga caccttcggc gtgacactga caaaattgaa
     1201 aagactttgt cacgccggca gtgggttcta atgcagtggc tcactatctc tcggacttaa
     1261 gagacctttg gtagcaattg gtgcgaggag ggggacttga acccccacgc catttctggc
     1321 actagcccct caaactagcg cgtctgccta ttccgccatc ctcgcaatct gatgaacgac
     1381 gactatttgg ttgatttctg tggttgagtc gcctgaggtg aattcggtgc cgtctgtgtc
     1441 ggtgcgctac cggttgcgtc gctctgtttt ggaaccggtg gtgtttccgg cgcggccggc
     1501 atagtctgag gagttggtgt tcgactatcg aaaatagtta ccgacggctt gtgtcccgca
     1561 aaataaccca gagttagcga cgtaatcatg aagacgatag ccattcccgt cgtcaacttt
     1621 gtaagaaaat tgcctgcacc actactgccg aagatagtgg agcttgagcc gccgaagacg
     1681 gcacccatgt ccgccttacc ggtttgcagc aaaacaacca gaatgaggcc gatgctaact
     1741 atcacgtgaa tgatggtaat tgcgataacc atatccgaat ccgcttgtaa aacgattcac
     1801 cacggcaaaa gatttctgtt cagaccgggg tcgcgtcctt acgactaccg atactccaac
     1861 accagttcct cggcgatttt atgttttcgt atatcatttt ccgcaaccgc cttcaatgcg
     1921 atcttaattg cttaggctta ccaaagttgc actcatggag tttcctcaag agctttgatt
     1981 ccggggagtt cttttccttc taaaaactca agcgtcgctc cacctcccgt agaaagatgt
     2041 gtgattcttg ccgcgacgcc ggctgccgtg actgcagcga cggtatcgcc accggcaatg
     2101 acgctgacta ggcgcggata accctccgcg agttttcgcg ccaatgcctg actgcccttg
     2161 ctgtagggct ccatttcaaa cagacccacc ggaccgttcc aaagcaccat tcctgcatgt
     2221 tgcaactcct gcgaaaattg agtgatggtt tgcggtccga tatccagccc catctgatcc
     2281 ggcgggatgt tgcctgtaca tatcgttgga ttcttccgtt cgctatcacc gatgatatgg
     2341 tccacgggca agaatgttga cacaccttgc tcagcagctt gttttagtag ctccttggcc
     2401 aatggcactc tgtctgtttc cactaaggat ttaccgatgt cgattccttt ggccttcaga
     2461 aatgtatagg ccatgccgcc accaatgagc agggtgttca ctttcggcag caggtttctg
     2521 atcacgccga tcttgtccga tactttagcg ccaccgagaa tagtgacaaa aggtttttct
     2581 gggttcaaaa gtaagcgtga gagatactca agttccttgt gaagcaacaa cccggctccc
     2641 ttttgcttga aaaatgctgc cattccagaa attgaagcat gcgcccgatg agccgcgccg
     2701 aaggcgtcgt caacatagac atcggccaaa gatgctagcg ccttcgagaa ggccatgtcg
     2761 ttgctttctt cttctttgtg aaagcgaagg ttctcgagca agataaggcg ctggcttggg
     2821 tcttggacca tgcgctcaac ctcagggccg atactgtcag gtgctagcaa tacgggttgc
     2881 ttcagggatt gttcgaggtg gtcgcgtatc ggtctcaagc tgtagtttgg cacaaccttg
     2941 ccgtccggtc tgcctaagtg cgacgcgacc aggagctttt tcgcccggtt caagatcaat
     3001 aggattgtgg gcagggcgct ttcgatacga tgagtctcga tgatcctacc ttgttcgatg
     3061 gggacgttga agtccacgcg caaaaagaca gtcttatctt ttagattaag gtcttcgact
     3121 ctccgaatca tacggcttcc acctctggaa gtaagttttc caacgctttg cggaaacaaa
     3181 atttatttcc caattgacta attatagtgc ttatattact ttttcaaata gttcctgaaa
     3241 tttgtaatga cttccctctg gttgctccgc ttgttggccc aagatgggct ggctgcatcc
     3301 ccccagcacg gcattcttga tttggttcgg ggatccggac ccgtcgttca atttattctt
     3361 tatctgctga ttttgttctc tgtcgccagt tggggtatca ttgtatacaa atttcgccag
     3421 ctcaggagag cgaagcgaga gtctgagaaa ttcatcgatg tcttttggga gcgacggaat
     3481 ctttcgtcga ttcacgaggc cagccgggac ctcaaagcgt gtcctgtggc gcaggtattt
     3541 cggtccggat atgaggaact ggtccgggta tcgcgcagca aaaaggaaaa tccttccggc
     3601 gaggccctga caacggagct gggtggagtc gagaatgttt cccgggcgat gaaacgagcc
     3661 accagcgtag agattaccaa gctggaaaaa tatctgactt tcctagctac cacggcaagc
     3721 gctgctccgt ttattggatt gtttggcacg gtatggggca tcatgaacgc ctttagagga
     3781 ctgagtgtga cgcattcttc gagcatccaa gcggtagcac ccggcattgc agaggctcta
     3841 atcgccactg cggtgggttt ggcggctgcg attcccgctc tcatggccta caactacttt
     3901 gtccagcaga ccaaagtgct tgccgctgaa atggacaatt tttcgcacga gtttctgaat
     3961 atcgctgagc gacatttttt taagtaactt tgggaggcca ttatggcgat ggatggagca
     4021 tcgcagcgcg acgcgacgac aatcgcacag attaatgtta cgccgctggt tgacgtgatg
     4081 ttggtgctgc tggtcatttt tatggtgacc gcacccatca ttcagcaggg cgtgcaggtc
     4141 aacctgcccc aggcgaaagc tggcgcgatt cctggaacgg aagaattatt ggtagtaacg
     4201 attgctaaaa atgggaaggt ttacctaaat gataacccaa tgactctggg agagttggga
     4261 gataagctgc gagccatccg caagttgcaa gcggacaaac aggtttatct gcgggccgat
     4321 caagacgtgc gatacgggtt ggtgatgaag gcggttgcgg aaattaaaca ggccgggatc
     4381 gagcggttgg gtatggttac gcgaccctct tctgatgaga aatagatcga tggcgggaca
     4441 tcaatccact caaaccataa gttcacagga acgatcgctt cccctcgttg atcgcgaaga
     4501 taagctttcg aagtggcttt ttttctcctt tctgtttcac ggtgctttga ttttggctct
     4561 tttcgtggtg ccgtttttgc cggcccggag tcggccgagc tatccggttt ataccgtgga
     4621 tttagtggga ggggaaaaga tcgggcggaa caatctaggt acggaactag tgcctccttc
     4681 ggctcccaag gcggccccaa aaaagacggc ggaggaagtt cctcctccac cggcgcagga
     4741 aactaagcag gagaccaaaa aggaaaagcc taaaccgata gagaaggagg cgccggcaga
     4801 agaaaaagtg gcgctcaaag aagctacaaa aaaggagccg ccgaagaaag aaccggccaa
     4861 agacaccaag agtgagtcca agactgacga atcggcgttg gatcgggtgc gggaaagatt
     4921 gatccaatcc gctgtggatc gtgcaaaaag tcgcacggaa ggggcgcaaa atccatctaa
     4981 aggagatgta atcagttcgg gtcagggcga gggtgaggga gcagcggctt tgggtccagg
     5041 cggacgtggc gggggcgttg tcaaaggtat ggagtttatc atctaccaga atcggatgct
     5101 cagcagcatt aaggacaatt gggcctgggt aggacagaaa agtaacctca aggtggtcgt
     5161 gcatttcagc atcaaagaca gtggggaaat cactggattg aaaattgttc agtcttcagg
     5221 agatcaatcc tatgacgaat cggtcctgag agccgtgaag aaatctagcc ctctgccgcc
     5281 accaccggag agttcccgga aagattttgc cgacgttgaa ttaacgtttc gaccaaaaga
     5341 cttgggagca tagcgtaatg atagtcaaaa ccattttcgc cgttctattg atgattctca
     5401 acccttggcc tgctaacgga caacccaagg gagacacggg agtaagagga gagatcgttg
     5461 gacagggcgg cctgaaattt cccatcgcgg tgtcgccgct gaaaaacttg ggaaccacag
     5521 ccgatgttgg aaaactgtct gaagggattg ccgacacgat tgtttatgac ctcgatctgt
     5581 cgggatggtt caaagtgtta gaccgcgccg cctatataga gaacccgcag aagagcggtg
     5641 tagccttggg gagctttgat ttcaaagatt ggtcaacgat cggggcggag ggcctagtca
     5701 aaggaggctt cacgattcaa ggagatgaag tcacagtgga attgcggctc tttgatgttt
     5761 tccaaaataa ggagcgggtc ggcaaacgct acgtcggtcg agtcaaggac tatcgacgca
     5821 tcgcccacaa atttgccgat gagattatca atcagttcac gggtgttccc ggaatcttca
     5881 atactcggat cacttatgtc tccaccagcg gaggccgctt taaggagatt catatcgctc
     5941 acctcgacgg aagcgaaaaa tttcaggtca ccaataacca tacgatcaac ctgtcgccct
     6001 catggacagc tgacggtaaa tcggtgctat acacctcgta caaggatcgc aatagtagcc
     6061 tttatctctt tgagctgtat agcggcaagg aggccaagat tacccctcgt aacggacggt
     6121 atttgggagg taaagcttcg cccgatggcc aattcatagc cgccactgtg gaaacgggag
     6181 gaaacagcaa tattgttttg ctggatcgaa atggtaatat gattcgtagc ctcacagaaa
     6241 gccccggtat tgaagtctcg ccggcgtggt cgccggacag caaacaatta gtatttgtgt
     6301 cagatcggag cggctcgcct caggtctatg tccttgatgt ggcgagtaac aaatctcgtc
     6361 gtctcaccta tagcggtaac tacaatacat cgcccgaatg gtcgccacga ggcgataggg
     6421 ttgcctacac cggcagagtc ggaaaccgtt tcgcaatttt tacgattggg gtcgacggcg
     6481 gagaatcacg gaagctcacg gcagagtcgg cggatagcga agatccgaca tggtcacctg
     6541 acggaagatt catcgcgttc tcttctaatc gagctggaaa gtatcatctc tatgtcatgc
     6601 aggcgaatgg agaaaatcaa cgtagattga caggttccgg gggagatgat acaaaaccta
     6661 gctggtcccc tcgtttagag tagtttgccc tcagttcatg tgtgtcgata tcccaaaaat
     6721 tgtgtgcgta tccgagataa ggagcgtgcg atggcggctg tgaataaact agtgggctgc
     6781 ggacttttg
//
LOCUS       DASSWZ010000013         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_990887, whole genome shotgun sequence.
ACCESSION   DASSWZ010000013 DASSWZ010000000
VERSION     DASSWZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990887"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1294
                     /locus_tag="VFN58_00285"
     CDS             86..1294
                     /locus_tag="VFN58_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8916451.1"
                     /translation="MTLPLSRDGWLLFATCGVRSFAYGFLSVVLGLYLDTIGLTTTAI
                     GWIFTAALAGGALMTIVITTVADSFGRKALLILGAVLMATAGWVFAISSDPLLLALAA
                     IFGTISPSGKEVGPFLSIEQAILPETTQDQHRTTVFSAYNLIGSLSGAVGALAVGLPS
                     LFSISQITGYRFLVWGYVICAVVMMVVFALLSPAIERKPKTASRKRVIGVQRSRKVVA
                     KLAGLFAVDAFAGGFIVQSIVAYWFYLRYETDLNTLGGIFFGTNLLAALSFLAAPAIA
                     RRFGLLNTMVFTHLPSNVLLLLIPLMPNLELAVVMLLIRNLLSQLDVPTRQSYAMAVV
                     DPDERAASAGILSVARNAGAAIAPLFTGPILTVPSLGLPFLLAGGLKIIYDLWIFAVF
                     RKVKPPEEEI"
     gene            complement(1331..2317)
                     /locus_tag="VFN58_00290"
     CDS             complement(1331..2317)
                     /locus_tag="VFN58_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8916452.1"
                     /translation="MEPEKFQQVFKRLEAEVRKVIVGHDDVIRQVLIAFFAGGHVLLE
                     GVPGLGKTLLVKSLSHVLGVSFKRIQFTPDLMPSDIVGTEVLTEANGRREFQFKKGPI
                     FAHVVLADEINRATPKTQSAVLEAMEEKQVTVFGESHTLACPFIVLATQNPIELEGTY
                     PLPEAQLDRFFFKLMVAPPSPIELREILKRTTGTETGDAGKVLPDDGGKLIGQMQQLL
                     RQVLIAPPIEDYVVRVVHATQPNLINGNGSTSPVKQYLRFGSSPRGAQAVILGAKGNA
                     LADGRIHVSYEDVAKVIYPALRHRIILNFQAEAENVNTDEILAEVVKQVPRA"
BASE COUNT          468 a          676 c          649 g          601 t
ORIGIN      
        1 taagtaatca tactggctgc gtgttgtgga ggggttaagg gccaggaaca acgacgggga
       61 ctcggggagt ctatctagtt taattgtgac cttaccattg tctcgggacg gatggctgct
      121 atttgccacc tgtggtgtgc gctccttcgc ctacgggttt ctgtccgttg ttctgggact
      181 ctatctcgac accattggac tcacgaccac ggccattggt tggatcttca ccgccgcgct
      241 cgctggtggc gctctgatga caattgtcat tacgactgtg gccgacagtt ttggtcgaaa
      301 agcattgctg attcttggcg ccgtactcat ggcgactgca ggttgggtct ttgctatcag
      361 tagtgatccg ttgctgcttg ccttagctgc gatctttggc accataagtc cttccggaaa
      421 agaagtcggc ccgtttcttt ccatcgaaca ggcgattctt ccggagacaa ctcaagatca
      481 acaccgcacg actgtattct ccgcatacaa tctcatcgga tcgctctccg gcgcggtggg
      541 tgcattagct gtcgggttgc cgtccctgtt ttcaatttcg caaattactg gttatcggtt
      601 tctagtctgg ggttacgtca tttgcgccgt cgtgatgatg gtcgtattcg ccttactatc
      661 accggccatc gagaggaagc ccaaaacagc ttcgcggaag cgagtaatcg gcgtacaacg
      721 atcccgtaag gtcgtggcga agctggcggg acttttcgcc gtggatgcct tcgccggtgg
      781 tttcatcgtg caaagcatcg tcgcgtattg gttctatctc cgctacgaaa ccgatctgaa
      841 cacattgggc ggaattttct tcggcacgaa tcttctggcc gccctctcct tcctcgccgc
      901 ccccgcgata gcgcggcgtt tcggcctgct gaacaccatg gtttttactc atctaccgtc
      961 aaatgtcttg cttctcctca taccgttaat gcccaacctg gaactggctg tcgtaatgct
     1021 gctgatacgg aacttactct cgcagctcga cgtacccacc cggcagtcgt atgcgatggc
     1081 tgtcgtcgat cctgacgagc gagcagcttc ggctggaatc ctttccgtcg ctcgcaacgc
     1141 tggcgcggcc atcgcgccgc tttttaccgg gccaattcta acggtcccgt ccttgggtct
     1201 gccgttcttg ctggcaggcg ggctgaagat tatttacgac ctttggatct tcgcggtgtt
     1261 tcggaaggtg aaaccaccgg aggaggaaat ctgacaccgg aagacaagca cggcagagaa
     1321 agagcctatc ctaggcgcgc gggacctgct tcacaacctc ggcgagaatt tcgtctgtgt
     1381 tgacattttc cgcttccgct tggaaattga ggatgatgcg atggcgcagc gccggataaa
     1441 tcaccttagc gacgtcctcg tagctgacat ggattctgcc gtcggccagc gcattgcctt
     1501 tggctcctag aatgacggct tgggcgccgc gcgggctaga accaaaacgg agatactgtt
     1561 taactggcga ggtcgagccg ttgccattaa ttagattagg ctgcgtggca tgcacgacgc
     1621 gcacgacata gtcctcgatg ggcggagcga tcaatacttg acgcagcagt tgttgcattt
     1681 ggccaatcag ttttccgcca tcgtccggga gcaccttgcc cgcatctccg gtctccgtgc
     1741 cagtcgtccg cttgaggatc tccctcagct ctatcggtga cggcggtgca accataagct
     1801 tgaagaaaaa tcgatccagc tgcgcttcgg ggagcggata cgttccctct agctcgatcg
     1861 gattctgcgt ggccagaact atgaacgggc acgccaaggt atgggattcg ccgaagaccg
     1921 ttacttgctt ttcctccatc gcctccaaga ccgcagactg ggtcttcggc gtcgcacggt
     1981 tgatctcatc cgcgagcacc acatgggcga atatcggtcc ttttttgaat tgaaactcgc
     2041 ggcggccatt ggcttcggtc aaaacctctg tgccgacgat gtcggagggc atcagatccg
     2101 gcgtgaattg aatacgcttg aacgaaacgc cgaggacgtg actcagggat ttcaccaaca
     2161 gtgtcttgcc tagacccggt accccctcca gcaagacgtg tccgccagcg aaaaaagcga
     2221 tcaacacttg cctaatgaca tcatcgtgac cgacgataac tttgcgtacc tcggcttcca
     2281 accgtttgaa gacttgctga aatttctccg gctccatcag ggcccctttc tggctcagcg
     2341 aataatgccg cggtactcca aaggcagttg ttgtttctcc atcggggcct ccgg
//
LOCUS       DASSWZ010000014         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1217290, whole genome shotgun sequence.
ACCESSION   DASSWZ010000014 DASSWZ010000000
VERSION     DASSWZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217290"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFN58_00295"
     CDS             complement(<1..1076)
                     /locus_tag="VFN58_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET8916453.1"
                     /translation="MKRYICIHGHFYQPPRENPWLESVELQDSAYPYHDWNERVTAEC
                     YAPNSVSRILDGDNQIIQLVNNYSKISFNFGPTLLAWLEAKAPDVYDAILEADRESQK
                     NFSGHGSALAQVYNHMILPLANARDRRTQILWGIRDFQRRFKRLPEGMWLPETAVDIE
                     SLEILAELGIRFTILAPHQADRVRPIGGRVWHDVSGSRIDPTQAYEQRLPSGRKIALF
                     FYDGPIARAVAFEGLLSSGEHLANRLVGAFDDGRNRPQLVHIATDGESYGHHHRFGDM
                     ALAYALNTIESNQLAQLTNYGEYLERHLPTHQVEIIEKTSWSCFHGIDRWWSNCGCNS
                     GGRPGWNQEWRTPLRGALDTLRDSL"
     gene            complement(1173..2420)
                     /gene="glgC"
                     /locus_tag="VFN58_00300"
     CDS             complement(1173..2420)
                     /gene="glgC"
                     /locus_tag="VFN58_00300"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET8916454.1"
                     /translation="MYVLGIVLAGGRGERLYPLTAHRAKPAVPFGGRYRIVDFVLSNF
                     VNSGILSIYVLTQFKAQSLLEHLEKGWRTTDFLGDHFITPVPAQMRMGKDWYQGTADS
                     VYQNLYLIERHRPRLVAVFGADHIYRMNIRQMIDEHQKKGADVTVAALPVRIEEASQF
                     GVLEVDSDWRIVGFDEKPSQPKSIPGEPDSALVSMGNYLFNTDTVVETLTQDAKLESS
                     NHDFGRDILPSLTNGKGVYAYDFRKNRVPSTQKGEEPSYWRDIGTIEAYYEANIDLCS
                     LDPSFNLYNRGWPLRTTDFGDPPAKFVFDWFDRKGMALDSIVSQGTIISGSVVKNCVV
                     GRNVRIHSYSQIEGSVIMDWAEIGRGCKIRRAIIDKSNVIPPGTEIGYNIEKDRQRYF
                     VSESGIVVIGRGERRTSWVMTGQ"
     gene            complement(2447..3874)
                     /gene="glgA"
                     /locus_tag="VFN58_00305"
     CDS             complement(2447..3874)
                     /gene="glgA"
                     /locus_tag="VFN58_00305"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET8916455.1"
                     /translation="MLSSEIAPFAKTGGLADVLGTLSVALERRGHEISLIMPAYRCVL
                     NGPFALDETGMQLAVPLGPSAAEASLLKAQLGQNVSVYLVRADRYFDRDFLYGTPYGD
                     YPDNAERFAFFSRAALEILRRHPVEIVHCHDWQTALGIVFLKAQGARYPEVASVKTVL
                     TAHNLGFQGIFRESNWPLLDLDRSYFSPQHLEFYGNINFLKGGLVFADKITTVSPTYA
                     QEIMSAEQGFGLQGILRQRAADVTGILNGVDYNEWSPETDLFIARRYSGNDLTGKPIC
                     KDKLQASMGLPVKSAVPLFGMVSRLTSQKGFDLVQKIFDRMMERELQLVLLGSGELRY
                     EDFFVTAAARFPEKFAVRMGFDEALAHQIEAGADIFLMPSLYEPCGLNQMFSLKYGTL
                     PVVRAIGGLKDTVIDYDAEPGKGTGFVFDRYDPNALWESIERALRAFTDRRSWAALMH
                     RAMRMDFSWDRSAQAYSNLYEQLLR"
     gene            complement(3935..>4678)
                     /locus_tag="VFN58_00310"
     CDS             complement(3935..>4678)
                     /locus_tag="VFN58_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HET8916456.1"
                     /translation="KWDMGWMHDTLEYMSQDPIYRRYHHNKLTFRMIYAFHENFVLPL
                     SHDEVVYGKRSLIGKMPGDDWQKFANLRALFGYMYAQPGKKLLFMGGEFGQWREWVHD
                     GSLDWDLLGYPLHAGLQRWVEDLNRLYRSEPALYEMDCDPAGFEWIDCDDADSSTVTL
                     IRKGKSSSTIILVVCNFTPVPRYSYRLGSPRSGFWQEILNSDAGEYGGSNMGNLGGVE
                     TVPVPLHGRPYSLTITLPPLSVSFFKHQG"
BASE COUNT         1096 a         1280 c         1248 g         1054 t
ORIGIN      
        1 agggagtcac gcaatgtatc cagcgcaccc cgtaatgggg tgcgccactc ctgattccag
       61 ccgggacggc cgccggagtt acaaccgcag ttgctccacc agcgatcgat gccgtgaaaa
      121 caactccagg aggttttttc aataatctcg acctggtggg tcgggagatg ccgttcgagg
      181 tattcaccat agttggtaag ttgtgccaat tgattggact cgatggtgtt caaggcgtag
      241 gccagggcca tgtcgccgaa gcggtggtga tggccgtaac tttcaccgtc cgtagcgata
      301 tggacgagtt ggggccggtt gcgcccatcg tcgaacgcgc cgacgagccg gttggccaga
      361 tgctcgccgc tggagagcag tccttcaaag gcgacagcgc gagcgatcgg tccgtcataa
      421 aaaaacagag caatctttcg ccccgaaggc agccgttgtt cataggcctg ggtcggatcg
      481 atgcgagagc cgctgacatc gtgccagacg cgtccaccga tgggacggac gcggtcggct
      541 tggtgcgggg caagaatggt gaaacgaatc cctagctcgg caaggatctc caggctctca
      601 atatccacag ccgtttccgg cagccacatg ccctccggca gacgcttaaa gcgtcgttga
      661 aagtcacgga tgccccatag aatctgcgta cgccgatcgc gcgcattagc cagcggcaga
      721 atcatatgat tgtagacctg ggccaaagcg gagccatggc cggaaaaatt tttctgactt
      781 tcccgatcgg cttcgagaat cgcgtcataa acatccggcg ctttcgcttc aagccaagcg
      841 agcaaggtag ggccgaagtt gaaactgatc ttggagtaat tgttgaccag ctggatgatt
      901 tggttgtcgc cgtcgagaat ccgcgacacg gaattcggag catagcactc cgccgtaacg
      961 cgttcgttcc aatcatgata agggtatgcc gaatcctgca gttcgacgct ttcgagccaa
     1021 ggattctcgc gagggggttg gtagaaatgg ccgtgaatgc aaatgtagcg tttcatgaga
     1081 agctaaatca cggcctgact ctgattgtaa attgtttcaa caggtcttcg gtgtcgtccg
     1141 gcgtgctatc cgagttagaa tcctcgcggt tattattgac cggtcatcac ccagctggtt
     1201 cttcgctcgc cgcgcccgat caccacaatc ccgctttcag aaacaaagta gcgctggcga
     1261 tctttttcta tattgtatcc aatttcagtt cccggtggga tgacgttcga tttgtcgata
     1321 atcgcacggc gaattttaca accccggccg atttccgccc agtccatgat caccgagcct
     1381 tcgatttggc tgtaactatg aattctgaca ttgcggccga caacgcagtt cttgaccacg
     1441 ctgccgctaa taatggttcc ttgagagaca atcgagtcca aggccatgcc cttgcgatcg
     1501 aaccagtcaa agacgaactt ggccggtgga tcgccaaaat ctgtagtacg aagcggccaa
     1561 ccgcgattat ataaattgaa cgacggatcc aaagaacaga gatcgatgtt agcctcgtag
     1621 taagcttcga tggttccgat atcgcgccag taactgggct cctcgccttt ctgcgtagac
     1681 ggcacgcggt ttttgcgaaa gtcgtaggcg tagactccct ttccattagt cagcgatggc
     1741 aggatatcgc ggccaaagtc atggttgctc gattctagtt ttgcgtcctg cgtcaaagtc
     1801 tcaaccaccg tgtcggtatt aaacagatag ttgcccatgg agaccagtgc ggagtcaggt
     1861 tcgccgggaa tcgatttggg ctggcttggt ttctcgtcaa agccgacaat gcgccaatcg
     1921 gagtcgactt ctaacacccc aaactgactt gcctcttcga ttctaaccgg caacgccgcg
     1981 acggtgacgt cagcgccttt tttttggtgc tcgtcaatca tctggcgaat gttcatccgg
     2041 tagatatggt ctgcgccgaa gacagccaca agccggggtc tgtgtcgttc gatcaggtac
     2101 aagttctgat aaacagagtc ggcggtcccc tgataccaat ccttgcccat gcgcatttgg
     2161 gcaggaaccg gcgtgataaa gtgatctccc aaaaaatcag tggttcgcca acccttttcc
     2221 agatgctcca acaaagattg cgctttgaat tgggtcagaa cgtagatcga aaggatgccg
     2281 gagtttacga aattggaaag gacaaaatcg acaattctgt accggcctcc gaacggcacg
     2341 gcaggcttcg ctcggtgtgc cgtcaaggga tagagacgct cgcccctccc gcccgcgagg
     2401 acaattccca agacatacat cattgcacct ctgttttgtt acctcctcaa cgcaaaagct
     2461 gctcgtacaa gttgctgtat gcttgagctg agcgatccca agaaaagtcc atgcgcatcg
     2521 ctcgatgcat caacgcggcc cacgatcggc gatcggtaaa agctcgtaat gctctttcga
     2581 tgctctccca taacgcgttg ggatcgtatc tgtcaaagac aaatccggta cccttaccag
     2641 gctcggcgtc atagtcgatc accgtgtctt ttaggccgcc gattgcccgg acgacgggca
     2701 gtgtaccata tttcaagctg aacatctggt tgaggccgca gggttcgtaa agcgacggca
     2761 tcaagaaaat gtcggcgccc gcttcgattt gatgcgctaa ggcctcgtcg aaacccatgc
     2821 gcacagcgaa tttttcggga aaacgggcag cggcggtgac gaaaaaatcc tcatagcgca
     2881 attcgccgct ccccaaaagc accagttgaa gctcccgctc catcatccga tcaaagatct
     2941 tctggacgag gtcgaagcct ttctgggaag ttaaacgaga aaccatgccg aacagcggca
     3001 ccgcggactt gacggggaga cccatgctcg cctgcagttt gtccttgcag ataggcttcc
     3061 cggtgaggtc attcccactg tatcgtctag cgataaagag atcggtttcc gggctccatt
     3121 cgttgtaatc tacgccgttg agaattcccg tcacatcggc cgcgcgctgc cgcaatatcc
     3181 cctgcaagcc gaagccctgt tccgcgctca tgatctcctg cgcgtaagtt gggctcacgg
     3241 tagtgatctt atcggcaaag accaggccac ccttaagaaa attaatgtta ccataaaact
     3301 ccaagtgctg tgggctgaaa tagctgcgat caaggtccag taagggccag ttcgactccc
     3361 gaaaaattcc ctggaagcca aggttgtgcg cggtcagaac ggttttgacc gatgcaactt
     3421 cagggtagcg agcgccttgg gccttcaaaa agacaatccc cagcgcggtc tgccagtcgt
     3481 ggcaatggac gatctcgacg ggatgccggc ggagaatttc taacgcggca cgggaaaaaa
     3541 aagcaaacct ttccgcatta tcgggataat cgccataagg tgttccgtat agaaaatcgc
     3601 ggtcaaaata acggtcggcg cgcaccagat atacggacac gttttgcccc agctgagcct
     3661 tgagaaggga agcctctgcc gctgacggcc ccaacggaac agccaattgc atcccggtct
     3721 cgtcgagcgc gaaaggcccg ttgagtacgc agcgataggc gggcatgatt aaagaaatct
     3781 catgcccgcg cctttcaagc gctacggaaa gcgttccgag aacatcagcc aagccgccgg
     3841 ttttggcgaa gggggcgatc tcggatgaga gcatcgcaat tttcatggac aggtgacgac
     3901 aagcgcttag acttccgaca ttctcctgtt ttttctatcc ttggtgctta aaaaaagaaa
     3961 ccgacagcgg cggcagtgtg atcgtgagag aataggggcg gccatggagt ggcacgggaa
     4021 ccgtctctac tccgccgaga tttcccatgt tgctgccacc gtattcgcct gcgtcgctgt
     4081 tcagtatctc ctgccagaat ccactgcgcg gtgaaccgag ccggtaactg tagcgcggta
     4141 cgggcgtaaa gttgcagacc accagaataa tcgtcgagct cgatttccct ttgcgaatta
     4201 aagttaccgt gctgctatcg gcgtcgtcac agtcgatcca ttcaaaaccc gccgggtcgc
     4261 aatccatttc gtatagcgcg ggctcgctgc gatagaggcg attgaggtct tctacccatc
     4321 tttgcagtcc ggcatggagc ggatagccca acagatccca gtccaagctg ccatcatgca
     4381 cccactcccg ccattggccg aattcgccgc ccatgaacaa cagcttcttt cctggttgcg
     4441 catacatata accgaatagc gcccggagat ttgcaaactt ctgccagtcg tcaccgggca
     4501 tctttcctat cagtgaacgt ttgccgtaga caacctcgtc gtgggatagc ggcaagacga
     4561 aattttcatg aaacgcataa atcatccgaa acgttagttt attgtggtga tagcgccggt
     4621 agataggatc ttggctcatg tattccagtg tgtcatgcat ccagcccatg tcccactt
//
LOCUS       DASSWZ010000015         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_948494, whole genome shotgun sequence.
ACCESSION   DASSWZ010000015 DASSWZ010000000
VERSION     DASSWZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948494"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFN58_00315"
     CDS             <1..213
                     /locus_tag="VFN58_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916457.1"
                     /translation="ILNSVISLYYYAKIVKTMFLDTPDPGDKSLTITANNFTLLIPLT
                     ALTIVLGVYFSPLVYYTSQSLRFFMK"
     gene            335..1759
                     /locus_tag="VFN58_00320"
     CDS             335..1759
                     /locus_tag="VFN58_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8916458.1"
                     /translation="MTIAAKIKSFLFQEASLTTRFAVHSLICISILTVVLWFLVSNYL
                     INGILRREWETTAQFVRTEVREFLTAEDFTTKDRKPVGPKFAALLRHITLMPDILRFN
                     VYNTQGVAIWAAVDKKLVGRSFADNDKLQEAIQGKVVANMSSLSQKENVSERDSSKKV
                     VEVFVPIYSEGTRELLGVMEIHKRADSIDRDIREARIVVLLGALCGGLLLYLSLFAIV
                     RQAARKIKEQEENLLKVQSDLVASQRMAAVGEMAGAVAHGIGNPLSSIRAAAQVAKLD
                     CDECQGPDLQQKTLTTLDEIIQQVDRVQKRMRGLLNFAKPMEPRQTPVEINSLLSDVV
                     DVLRPRFGAAGVSLQLDLEKSLPKAQLDANHVEQIFMGLITNALEATPKGGRVTIRTH
                     TLTGNGSATRINVSIEDTGEGIPVESRERVFAPFFTTKPDGTGIGLSLARKFVERNRG
                     KITVSDGISGGAKFDVIFPVGGSN"
BASE COUNT          528 a          558 c          619 g          521 t
ORIGIN      
        1 atcttgaaca gtgtcatatc gctctactat tacgccaaga tcgtcaagac catgtttctc
       61 gacacgcccg accccggcga caaatcgtta accatcacgg cgaataattt cacgctcctc
      121 atccctctaa cggcactcac gattgttctc ggggtttatt tctcacccct ggtttattac
      181 accagccaat ctctgcgttt cttcatgaaa tagtatttgg cgcgccggat tggtatggcc
      241 caagccgagg cccttccacc gttttcgttc ggtttgaccc tctcagtaag ctggtgctag
      301 cttaggagcc gatctagagg gccttgctac ttcgatgacg atagcggcaa aaatcaaatc
      361 tttcttattt caggaagcca gcctcaccac acggtttgcc gtccatagcc ttatctgtat
      421 cagcattttg acggtagtcc tgtggttcct ggtttccaac tatttgatca atgggatctt
      481 gcgacgggag tgggaaacga ccgctcagtt cgtcagaacg gaagtgagag aatttctaac
      541 ggccgaagat tttacaacca aagatcgaaa gcccgtgggc ccaaaattcg ctgccctttt
      601 gcgtcacatc acgctaatgc ccgatatcct gcggttcaac gtgtacaaca ctcagggagt
      661 tgcgatctgg gcggcggtcg acaagaaatt ggtcgggcgc tcttttgccg ataacgataa
      721 gctgcaggag gctattcagg gaaaggtggt cgcgaatatg agctccttga gccagaagga
      781 gaatgtttct gagcgcgact cttctaagaa ggtggtggaa gtctttgttc cgatctactc
      841 cgaggggacc cgggagctgt tgggtgtgat ggaaatccat aaacgagccg attccatcga
      901 ccgggatatt cgagaagcaa gaatcgtcgt gttgttggga gccctatgtg gcgggttgct
      961 cttgtatcta tcgttattcg cgatcgtccg gcaagcggcg cggaagatta aagagcaaga
     1021 ggagaatctt ctcaaggtac aatcggatct ggtggcttcg cagcgcatgg cggcggtggg
     1081 ggagatggcg ggggcagtag cccacggcat cggtaacccg ctttcttcca tccgggcggc
     1141 ggcccaggtg gcaaagctag actgtgacga gtgtcagggc ccggatctgc agcagaagac
     1201 attaaccacg ctcgatgaaa ttatccaaca agtcgatcgg gtgcagaaac ggatgcgagg
     1261 attgttgaac ttcgccaagc ccatggaacc gcgccagacg cctgtggaga tcaattcact
     1321 tttgagtgat gttgttgatg tcttgcggcc acgattcggc gcagcgggcg taagtctaca
     1381 gttggatttg gaaaaaagtc ttcccaaagc gcaattggat gccaatcatg tggaacagat
     1441 ttttatgggt cttattacca acgccctcga agccacgccc aaaggagggc gggtaaccat
     1501 ccggacccat actcttacgg gcaacggatc cgcgacccgc attaatgttt cgattgaaga
     1561 taccggggaa ggcatcccgg ttgaaagtcg agagagagtt ttcgccccct tttttaccac
     1621 caagcctgac ggaaccggca ttggcttgtc actggcgaga aaatttgtcg aacggaatag
     1681 gggaaaaatc actgtctcgg acggaatctc cggcggcgca aagttcgatg tgatattccc
     1741 tgtgggcgga tcaaactgag cccggattcc gcagttggaa gtgtaaacag agagcgaggc
     1801 gcaagccttc cgcagcgaag tcaatgggcc tctgcctccg agcaaagttg cgatccgtgg
     1861 tgcgatgagt ggagttctca atcattcggg aacggactgc cgagtaccaa aaacgacttt
     1921 gaaacctcac gcttctgcgc cttcgctact ccaggctcac gcctcgctca aatgtaggca
     1981 tgacgttaac tgcggaatcc gggctgagaa gaagggtaga ccgcgccggt gaatttgccg
     2041 gcggggaaga attcttcgcg cagtatctac gtcaacggta tcatgatgtg cttctggtaa
     2101 gctggccgct cgcaaagacg cctgtaccat gcatcaaaat tggcgagttt cggtcgttcg
     2161 ataggtaagt tgaaccagcg gtaggcccat actcctagag ggatatcgcc gagagtcaaa
     2221 tcgtcg
//
LOCUS       DASSWZ010000016        13774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1274013, whole genome shotgun sequence.
ACCESSION   DASSWZ010000016 DASSWZ010000000
VERSION     DASSWZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274013"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFN58_00325"
     CDS             complement(<1..1007)
                     /locus_tag="VFN58_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916459.1"
                     /translation="MNVLDRLGLQTVFLCARGLKRVWILTLAGWLTSCSSSTPAPTTS
                     QPPESFSELKQRISLIRELPFKREVSLAKESPNATRTTPERIFSDEYGAQSLVHISHA
                     YKRLGLLPESTDFPAALADYVRLDRIFYYDARRDLIVISPDTTPLVQAMTTAEPALNL
                     EQLPAVLALSRALQEQHFQWQGKLNGISLEDRRLAFRALAVGDTVLVGTAYLQGSQRT
                     TRPPDSARNLAGWATALEKMGSHLPQLLQQKLVFPYREGSQFVQWAHAAKGWTGVNAL
                     FADPPLSTSQILRPPKYYVKRENPLAIRASGLARQMMESATVDQTLGEYLVQLLLTSN
                     LT"
     gene            complement(1008..1715)
                     /locus_tag="VFN58_00330"
     CDS             complement(1008..1715)
                     /locus_tag="VFN58_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET8916460.1"
                     /translation="MFEQPVTRQNLSLDVLRSRALLLLLLLPLLFLAACSVPPSRVKL
                     PPSPASEGRISQTGIASWYGPGFHGKATASGAIYDQNDLTAAHQTLPLGTRVMVTNLD
                     TGSSAEVTINDRGPFAKGRIIDLSYGAGKALGMIGPGTIPVRVEVLEGSNNIQSIRTS
                     LDYTLQLGSFAQIENAQQLRDKLKTSYADVSIVPLQSRDVTYYRVQLGTYSNRNAAEE
                     RARQLSQAGFPVIIMEK"
     gene            complement(1832..2404)
                     /locus_tag="VFN58_00335"
     CDS             complement(1832..2404)
                     /locus_tag="VFN58_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET8916461.1"
                     /translation="MVILISETTAFFCFLAYASFFEWTLHKYLMHTPFWQYPFRAHAL
                     VHHGLFRTGAQYFLPDVKVIRKVRFAWWNAPFIILLHMPVLLWLQRTLDANILAGAVS
                     ALILYYFLYEYLHYCMHIPKRRWLETTAWFRWLDSHHHMHHKRHFNNLNVVLPLADIV
                     FGTLIPPKDHMALPDREDGATQVGLLLSES"
     gene            2713..3459
                     /locus_tag="VFN58_00340"
     CDS             2713..3459
                     /locus_tag="VFN58_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET8916462.1"
                     /translation="MSGHSKWSTIKHKKAAKDAKKGRLFTKLIKEITVAARVGGGDIN
                     ANPRLRTAVITARTNSMPGDNIERAIKKGTGELEGVSYEEIQYEGYGPGGAAILAQVL
                     TDNKNRTVSEIRRLFAKHGGNLGETGCVAWMFDKRGVITVEKSQIDEDRLMGLVLEAG
                     GEDVKDEGDLFEVVTQPEDFERVKETLDREKIALASAQITMVPKNTVTVDEKHVEQIL
                     KLTEELEDHDDVQNVSANFDIPSEYLEKAS"
     gene            3459..4037
                     /gene="ruvC"
                     /locus_tag="VFN58_00345"
     CDS             3459..4037
                     /gene="ruvC"
                     /locus_tag="VFN58_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET8916463.1"
                     /translation="MGDASDRSVRRAVVGRRILGIDPGSLVTGWGIVESFGNSLNQVA
                     CGTIATAGAQAQGARLSRIYRGIQQIIVRYHPDGVSLEKVFFARNPQSALKLGQARGV
                     ALLAAAENQLDVYEYSSNEIKSAVVGYGHATKEQVQKMVASLLHVSEKIAADAADALA
                     AAVCHVHRQAFQARIVEAAPDWQPATRRRRLP"
     gene            4091..4684
                     /gene="ruvA"
                     /locus_tag="VFN58_00350"
     CDS             4091..4684
                     /gene="ruvA"
                     /locus_tag="VFN58_00350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET8916464.1"
                     /translation="MIAQISGTLAHKLPGEVIVDVGGVGYLIFIPLNVFYRLPEVGGT
                     VSLYIHTHLREDALQLFGFRAAEEKQVFLLLNSVSGIGPKLAVNILSGIPVEELVEAV
                     KEGDQLRLVSIPGVGKKLAERMIVELRDKFTTLQSRPSEAIAQSNDSQLVQDAVSALV
                     NLGYRKAEVEKNVRDMIQTGKRTLEEVIKDVLRRMSR"
     gene            4726..5760
                     /gene="ruvB"
                     /locus_tag="VFN58_00355"
     CDS             4726..5760
                     /gene="ruvB"
                     /locus_tag="VFN58_00355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET8916465.1"
                     /translation="MQRPVAPKLVEDDANYDVNLRPRGFDEYVGQEQVKENLKVAIAA
                     ARGRNDVLDHILFHGPPGLGKTSLAYIISREMGVNIKPTSGPVVERPGDLAALLTNLE
                     EGDILFIDEIHRLNHVVEEVLYPAMEDYQIDILIGQGPAARSMKLPLKPFTLIGATTR
                     SGLLTSPLRDRFGQSFRLDFYSTEALAQILKRSASILGVAAEDDGTTEIARRSRGTPR
                     IANRLLRRVRDYAQVKAGGKITKPVTQEALQMLEVDPIGLDKMDTMLLLTLIDKFSGG
                     PVGIETLAAAIGEEKDTIEDVYEPFLIQAGFLKRTPRGRVATELAYEHFGRNAVSPKG
                     SSSKNQPSLF"
     gene            5814..6026
                     /locus_tag="VFN58_00360"
     CDS             5814..6026
                     /locus_tag="VFN58_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET8916466.1"
                     /translation="MAGFGKTLIYIGIVLVVLGLIFSAAGKIPWLGNLPGDITIQRER
                     FTFYFPLTTCILISVIVTLVLYFFRR"
     gene            6064..8295
                     /locus_tag="VFN58_00365"
     CDS             6064..8295
                     /locus_tag="VFN58_00365"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8916467.1"
                     /translation="MENSSTAEPIEVNLKLEEAKRRKREGLVILATTLMVLAFAFFEV
                     RMPETAPEYSLGNNIAFFLLINVNVILLALLVFLVVRNLVKLVFERKRRILGSRLRVR
                     LVLAFVALSLVPTLLLFFIAGGFVTRSFERWFDLQVENALQGSLEIGQTYYQNSANNA
                     LFYARQLSQRMTREGLFEAHRLGELRAFIQNKQREYNLGTVELFSPDYRSLVVAFNEQ
                     VPTGVTIKPENEFLNRALRGLEVTRTQAFGEGDIIRGGVPIYSADKRVLGVIVVDYYV
                     PKSITKRALQISRSYEQYKHLMFLQAPMKNSYILTLLLITLVIIFAATWCGFYLAKGI
                     TVPIQKLAEGTHEVAQGNWDYKIDSGGDDEIGTLVESFNQMTRELKQSNLELEQRKRF
                     VETLLANITAGVIAVDPTGRITTWNKTAEDMFGVDAAGVLGKNQEEVFQVGPLQVMRD
                     ILESVKGRESVERQIKISLTDQLLTVVVTAATLRDDEGNTMGVMVFLEDITQIQKVQR
                     MEAWREVARRIAHEIKNPLTPIQLSAERLRKRYAKLLDGNGAILDKCTSTIIQQVDEL
                     KNLVNEFSHFARLPSAHLTRNDLNGIVQEALFLFKEGHNDIRFQFRNEEIPPLELDRE
                     QIKRVIINLLDNAVAAVSENGEIRIATSHDRSRGVVYLEVADNGCGVAPEVRGRIFEP
                     YFSTKKNGTGLGLTIVSQIIEDHRGYIRVRPNEPRGTKFTIEFPVQTESDLVQNEKRT
                     MHS"
     gene            8304..9608
                     /gene="lpxC"
                     /locus_tag="VFN58_00370"
     CDS             8304..9608
                     /gene="lpxC"
                     /locus_tag="VFN58_00370"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET8916468.1"
                     /translation="MKERLGQKVIMIVDDEDKVRESVREVLADEGYRVVDTADGTGVL
                     ELIEQEKPGLVLLDIWMPQIDGIDLLKQIKSKEPAINVVMISGHGNIHTAVTATKFGA
                     FDFIEKPLSLDALLLTVQRALGDYSPGRSKEINGNGGRKSKKGKRSPTAARPETSSPS
                     IKQRTLKKSIVTSGQGLHSGMKTGLILHPLPPNSGILFTGLSGDVQVPAHLDHVGSTG
                     YATSLRAKGFAVGTVEHLLAVLHSYGITNLLVKVQGEIPILDGSAIEFCNLIDEAGVR
                     EESEEGSEIVVDRQYRIGSQGGESISIEPSDVFSVHYILNYPKPVGIQDYVYTHKDSA
                     GFKEEIAPARTFGFLRDIEELQQAGLVNGGKLNNCILIDDEKIVNTDLRFPNELARHK
                     ILDIMGDFYLLGKPVRGAIIAHMTGHSDNIALLRELRSGMGL"
     gene            9679..10128
                     /locus_tag="VFN58_00375"
     CDS             9679..10128
                     /locus_tag="VFN58_00375"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET8916469.1"
                     /translation="MASSVPFPRLFTVGEANALLPAVRGLTERIQENIQRLKNKSKIV
                     IRDQQLDPGAADFMDRLQENSEIARLVSEVKDGVEQIQSYGCICKGAEQGLVDFPCLL
                     GAEVVFLCWQQGEPTISYWHRIEEGFTGRRPLLDVEKTVLDKKTSYH"
     gene            10202..11374
                     /gene="queG"
                     /locus_tag="VFN58_00380"
     CDS             10202..11374
                     /gene="queG"
                     /locus_tag="VFN58_00380"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET8916470.1"
                     /translation="MIAHNKNALSLRVKEEAQRLGFELVGISPVKLPPHEESFAEWLR
                     QGFSGQLEYMKRTEEIRRNPQRLVPWAVSIVSVGMNYYTPFSRPAASPEPKGWISRYA
                     WGDDYHYLIKDKLESLLDSIRRICNSPVEGKAFVDSGPVLEREFAGLAGIGWIGKNTH
                     LISPQNGSWFFLGELFLSIPLAYDRPIRDRCGKCDLCLKACPTGAFVGPYVLDARRCI
                     SYLTIELKGFVPLQLRPLIGNHIFGCDICQEICPYNVKARPTTERAYAPREGLHAPDL
                     IPLLSLDEEEFRRRFRGSPILRAKRKGLLRNVAVALGNARSHEALPALIRALDDEETL
                     VRGHVAWALGQIGLPKGISALQKRLRIESDPTVKMEIEDAITEAMKPLNTETGSTA"
     gene            complement(11445..11894)
                     /locus_tag="VFN58_00385"
     CDS             complement(11445..11894)
                     /locus_tag="VFN58_00385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET8916471.1"
                     /translation="MVQPRARTKNVTSYRVIYGDTDQMGVVYYANYLRWFEKGRSEFL
                     RQIDLPYKTIEEQGLHFPVTEVSCRYSRSARYDDVITIATQLTSVGRATLNFDYTISR
                     EADSASLASGSTRHACVDADGRITRIPMNLAQRLARALVTPTESTEH"
     gene            12071..12541
                     /locus_tag="VFN58_00390"
     CDS             12071..12541
                     /locus_tag="VFN58_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916472.1"
                     /translation="MKGSANYMIGSVTALLLAVWMVACTSVQEQPKPAAPTVAQQPVP
                     AKQEEAKAPTKQESGKAETKTAKTKAVEVEGVISSVGQRTITFQLERKGKVREEVVGV
                     DDKTNIEKDGSKVKLGQLQETDKALINYEPDAYTPANSVKVIGKGQIQKVGGDD"
     gene            complement(12538..13440)
                     /locus_tag="VFN58_00395"
     CDS             complement(12538..13440)
                     /locus_tag="VFN58_00395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET8916473.1"
                     /translation="MNGHIGLIHSILPATADIQVTVPDNHPSTRNLGSERGGSGTIVD
                     PDGYILTVHYVTVGAASITVTLPDGEQHPGALAAQDQETGLALVKIPARGLPFLKPAA
                     LDSVALGQPAVIIASSGQAARRVSGGYVSSMDGYDGHWEYMLERTIRLTAPNPGFGGG
                     TMANFKGEIIGVVSLNLTEVGKFSLAIPIDYYLQNAQELKQYGQVRSRPRRPWLGFYP
                     QPLAGHLVVGGIVAGGPAERYGLKEGDIIIGVEKKEIRSRSELYHEMWKKRPGERISF
                     RILRDEQSFDLEIIAGDRSDRYRS"
     gene            complement(13445..>13774)
                     /locus_tag="VFN58_00400"
     CDS             complement(13445..>13774)
                     /locus_tag="VFN58_00400"
                     /inference="COORDINATES: protein motif:HMM:NF027524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HET8916474.1"
                     /translation="LKLHNLHIIKNTVLEKLYATGGFTLFSQNEYVTLVVDFLERLAP
                     EMVMHRLTGETYRAITVAPEWSINKIGVHNAIYKELEERDSWQGRFYPNEAEAPEPRD
                     RPPALEE"
BASE COUNT         3299 a         3375 c         3976 g         3124 t
ORIGIN      
        1 gtgaggttcg atgtcaggag caattgaacg agatattcac ccaatgtctg atcgacggtt
       61 gcgctttcca tcatttgccg cgccaagcct gaagcgcgga tagccagggg attttctcgt
      121 ttgacgtaat atttcggtgg gcgaagaatc tgcgaggtcg agagtggcgg atcagcaaag
      181 agagcattga ctccagtcca tcctttggcc gcatgagccc attgaacaaa ttgactgccc
      241 tcgcgatagg gaaatactag cttttgttgc aacagttggg gaagatgcga gcccattttc
      301 tccaacgcgg ttgcccatcc ggcgaggttc cgggctgaat ccggcggccg agtcgttcgc
      361 tgagaccctt gtagataggc ggttccaaca aggaccgtgt cgccgacggc caaagcgcga
      421 aaggcgagtc tgcgatcctc cagcgaaatt ccgttgagct ttccttgcca ttggaaatgc
      481 tgttcttgta gcgctcggga gagggccaat acggccggaa gttgctccaa attaagggcc
      541 ggctccgccg tcgtcattgc ctggaccaac ggagtcgtat ccggtgagat cacgatgagg
      601 tccctgcgag cgtcgtaata gaaaattcga tctaacctca catagtccgc gagagccgcg
      661 ggaaaatctg tggattcggg tagaagtccg agtcgtttgt aggcgtgcga gatatggacg
      721 agcgactgtg ctccgtactc gtcggagaaa attctttccg gcgtcgttct agttgcgttc
      781 ggcgattcct tggccaaaga aacttcacgc ttgaacggca gctcgcggat caagctgatt
      841 ctttgcttca gttcgctaaa agactcaggc ggctgagaag tcgttggtgc aggggtggag
      901 ctggagcagc ttgtaagcca tccggcgagg gtgagtatcc aaacacgctt taacccgcga
      961 gcgcagagaa aaacagtttg taaaccgagc cggtcaagaa cgttcaccta cttctccata
     1021 atgataaccg gaaatcctgc ctgtgacagt tggcgcgccc gttcttcggc ggcgttgcgg
     1081 ttggagtagg ttcccaactg aacacgataa tacgtgacat cccttgactg cagcggcacg
     1141 atggaaacgt ccgcataaga agttttgagc ttgtcgcgca actgttgcgc attctcaatt
     1201 tgggcgaaag atcccagttg caaggtgtag tcgagagaag tgcgaatcga ttgaatgttg
     1261 ttggatcctt cgagcacttc cacgcgcacc gggatcgtac cgggaccgat catcccgagg
     1321 gcttttcctg ccccatagga gagatcaata attcgaccct tggcaaaggg accgcggtcg
     1381 ttgatcgtaa cttcggcgga gctgccggtg tcgagattgg taaccataac gcgagtgccg
     1441 aggggtagtg tctggtgcgc tgcagtcaga tcgttctgat catagatggc tccactagcg
     1501 gttgctttcc catgaaaacc tgggccgtac caagatgcga tgccggtctg gctaatacgg
     1561 ccctcggagg ctggtgaggg tggcagtttg actctagacg gaggcacgga acaggccgcg
     1621 agaaacaaca atggcaggag caacaataga aggagagcac gcgatcgcag cacatccagc
     1681 gatagatttt gccgtgttac gggttgctcg aacatggacc gccggattat agcggaaacg
     1741 gaagaaaaag gaaaaaaggg atcgacatga gacatttagc ctgcattcga cgctcacttc
     1801 tccatctccc aaggtctatg agttcgttgg cctacgattc ggataacaac agaccgacct
     1861 gcgtcgctcc gtcttcgcgg tccggcaggg ccatatgatc cttgggcgga ataagcgtgc
     1921 cgaagacgat gtcggctagc ggcaacacca cattgagatt attgaagtgt cgtttgtgat
     1981 gcatgtggtg atgggagtca agccaacgga accaggccgt cgtttctagc cagcgtcgtt
     2041 tgggaatatg catgcagtaa tggaggtatt cgtaaagaaa atagtagagg atcagcgctg
     2101 acacggcgcc tgccaatatg tttgcgtcca gcgttctttg taaccagaga aggacgggca
     2161 tgtgaagcag gataataaac ggggcgttcc accaggcgaa gcgaaccttt cggatgacct
     2221 ttacatcggg aagaaaatac tgggcgccgg tccggaaaag accatggtga accaaggcat
     2281 gagcgcgaaa gggatattgc caaaacggcg tgtgcatcaa atacttgtgt agcgtccatt
     2341 cgaagaaact cgcgtaggcg aggaaacaga agaaagctgt tgtctcgctg atgagaatga
     2401 ccattaaact cctttcaatc gcctcaatcg tccatgggta cagtgatccc tacttctacg
     2461 ccttcgggat agagttccgc cggaggccgg gtggcaagaa tgtttgggaa tcgcttcgca
     2521 gggagaggtc tattagagtc ttgagccgct tcggtcgtta tttcagactg gatcttgagt
     2581 tccgagtaat gtacgtgttt ttcgacgact cgtcaaacaa atctacaact attggatgtt
     2641 cgatgataca tggtttacgc agcagaggct ttgtggtagc ttaacgccaa ctaattcagg
     2701 gagttgttgt ttatgtccgg tcattcgaaa tggagcacca tcaagcacaa gaaagcggca
     2761 aaggacgcaa agaagggaag actcttcacg aagctcatca aagaaattac ggtggcggct
     2821 cgggtgggcg gcggcgacat caacgccaac ccgcgcttgc gcacggcggt gatcacggcg
     2881 agaaccaaca gcatgccggg agacaacatt gagcgggcga tcaaaaaggg cacgggagaa
     2941 ttggaaggcg tctcatacga agaaatccag tacgaaggat acggtccggg cggcgccgcc
     3001 atcttggccc aggtactgac ggacaataag aaccgcacgg tttctgaaat acggcgcttg
     3061 tttgccaaac atgggggaaa tttgggagag accggctgcg tggcctggat gtttgacaaa
     3121 agaggtgtca ttacggtgga aaaatcacaa atcgatgaag accgcttgat gggtttggtg
     3181 ctggaagcgg gcggagaaga tgtcaaagat gagggcgatc tatttgaagt ggtgacccaa
     3241 ccggaggact tcgaaagagt caaagagacg ctcgaccgag aaaagatagc ccttgccagc
     3301 gcgcaaataa cgatggtccc gaaaaatact gtaactgtag acgaaaagca tgtagaacaa
     3361 attctcaagt tgacggaaga gcttgaggat catgatgacg tgcaaaacgt ttcggcaaat
     3421 ttcgatatcc ccagcgagta cctcgaaaag gcgagctgat gggggatgcc tcggaccgtt
     3481 cggtgcgccg ggccgtggtg ggtcgtagga tcctgggcat cgatccggga tcgcttgtga
     3541 ccggatgggg cattgtcgag tcattcggaa attcgttaaa tcaggtggcc tgcggcacca
     3601 tcgcgacggc gggcgcccaa gcgcaaggtg ctcggttaag ccgcatctac agaggcattc
     3661 agcaaatcat cgtgcgttat catccggacg gcgtcagtct ggagaaggtt ttttttgcgc
     3721 gcaacccgca gagcgccctc aaactcggac aggcgcgcgg cgtggccctg ttggcagctg
     3781 cagaaaacca actggacgtc tacgagtaca gttcaaatga gatcaagtcg gctgtggtcg
     3841 ggtatggcca cgcgaccaag gagcaagtgc aaaaaatggt cgcgtcgctt ctccatgttt
     3901 cggagaagat agccgccgat gccgccgatg ctttggcagc cgcggtttgt cacgtccatc
     3961 ggcaggcatt tcaagcccgc attgtggaag ccgcgccgga ctggcaaccg gcgacgcgcc
     4021 ggcgcaggtt gccctgagcg attcaagcga ttggcgggaa gtcgcgtcct tagagaaatc
     4081 caagaccact gtgattgcgc aaattagcgg aaccctggcg cataagctgc caggagaagt
     4141 catcgtcgat gtcggaggag tgggctatct gatattcatc ccgctcaatg ttttctatcg
     4201 actgcccgag gtcgggggga ccgtcagtct ttacattcac acccatctaa gggaagatgc
     4261 gcttcagttg ttcggctttc gagcagccga agaaaagcag gtttttctct tgcttaacag
     4321 cgtttccggc atcggtccaa agttggccgt aaacattctt tccgggattc cggtggagga
     4381 attggtggag gcggtcaagg aaggcgatca gctgcggctg gtgtcgattc cgggagtcgg
     4441 caaaaagctt gcggaacgta tgatcgtcga actccgggac aaatttacga ctttgcaatc
     4501 ccggccgtcg gaagctatcg ctcaaagtaa cgattctcaa ctcgtccaag atgcagtgtc
     4561 ggcgctggtc aatctggggt atagaaaagc ggaagtcgag aaaaacgtgc gcgatatgat
     4621 ccagacggga aaacggactt tggaagaagt gatcaaggac gtgctccgac ggatgagtcg
     4681 ttaacacccg caatggcgaa gcttaacgac atcactgaga cctcgatgca gcgacctgtc
     4741 gcgcccaagc tggtcgagga cgacgcaaac tatgacgtca acctgcggcc gcggggcttc
     4801 gacgaatacg tcggtcagga gcaggtgaag gagaatctta aagtcgcgat tgccgcggcg
     4861 cgcggccgca acgatgtctt ggatcatatc ttgttccatg gaccgccggg gttgggcaag
     4921 acatcgctcg cctacattat ctcgcgggag atgggcgtga acatcaagcc tacctcggga
     4981 ccggtggtgg agcgccccgg cgatctggcg gcgctgctga cgaatctcga ggaaggcgat
     5041 attctgttta ttgacgagat tcaccgcttg aatcacgtgg tggaagaggt tctttaccca
     5101 gccatggaag attaccagat cgatatcctg attggtcaag gtcccgcagc caggtccatg
     5161 aagctgccgt tgaagccatt tacgttgatc ggagcaacga cccggtccgg acttcttacc
     5221 tcaccactgc gcgatcgctt tgggcaaagt tttcggctcg atttctattc gaccgaagcg
     5281 ctggcgcaaa ttcttaaacg atccgcctcc attttgggcg tcgctgcgga agacgacggg
     5341 actaccgaga tcgcccgccg gtcccggggc acgccgcgga ttgcgaaccg actgctgcgc
     5401 cgggtgcgtg attacgccca ggtcaaggcg ggcggcaaaa ttaccaaacc ggttacgcag
     5461 gaagctctgc agatgttgga agttgatccc atcggtctcg acaagatgga cacgatgctg
     5521 ctcctgacgt tgatcgacaa gttttcgggc gggccggtcg ggattgaaac gctggcggcg
     5581 gctatcggag aagaaaagga caccatcgag gacgtgtacg agccgttctt gattcaagcg
     5641 gggtttttaa agcgaacgcc ccgcggccgc gtggcgacgg agctggcgta cgagcatttc
     5701 ggacggaacg cggtatcgcc aaaaggcagc agttccaaga accaacctag ccttttttga
     5761 aataggtttt ggtcaccccg cgctgacaaa aaggcgagaa gcgaattaca caaatggccg
     5821 gatttggtaa gacgctgatc tatattggaa tcgtcctggt cgttcttggg ctgatctttt
     5881 cggcggctgg caagatcccc tggttgggga atctgccggg tgatatcacc attcagcggg
     5941 agcgattcac attctatttc ccgttgacca cctgcatcct tataagcgtt attgtgacgc
     6001 ttgtccttta tttttttaga cggtaaaatt tgactgtctt tttcccccct tagaaagctg
     6061 ctcatggaaa attcgagcac cgctgagccg atcgaggtaa acctcaaact ggaagaagcc
     6121 aagcgtcgca agcgtgaggg tttggtgatc cttgccacta ccttaatggt attggcgttt
     6181 gcgttttttg aagtccgaat gcccgagacg gcgccggaat actccttggg caacaatatc
     6241 gctttctttc tgttgattaa tgtgaacgtt atccttttgg cgctgctggt ttttctcgtt
     6301 gtccgcaacc tggttaaact tgtcttcgag cgcaagagaa ggattcttgg ttcccgcctg
     6361 cgggtgcggt tagtattggc atttgttgcg ttatccctcg tgcccacgtt gctgctcttc
     6421 ttcattgcgg gtggattcgt tacgcgttct tttgagcgat ggtttgatct tcaggtagag
     6481 aacgcgttgc agggctcatt ggagatcggc cagacttatt atcaaaattc ggccaataac
     6541 gcgttgttct atgcgcggca gctgagtcag cggatgactc gagagggact ctttgaggcg
     6601 catcgacttg gcgagctcag ggcgtttatc caaaacaaac agcgggagta taacctcgga
     6661 accgttgaat tgttctcgcc tgattatcgg tcgctggtcg ttgcgttcaa cgagcaagtt
     6721 cctacggggg tcacgatcaa acctgaaaac gagttcctca accgggcgct ccgaggtctg
     6781 gaggtgacgc gaacccaagc gtttggagaa ggggacatta tccgtggggg ggttcccatc
     6841 tacagcgcgg acaagcgcgt gctcggagtc attgtggtcg attattatgt gcccaaaagc
     6901 atcactaaaa gggccttgca aatttcccgt tcctacgaac agtacaaaca cctgatgttt
     6961 cttcaggcgc cgatgaaaaa tagctatatt ttgaccctac tcctcatcac gttggtgatt
     7021 atcttcgcgg ccacttggtg cggattctac cttgccaaag gcattacggt cccgatccaa
     7081 aaacttgcgg aaggaaccca cgaagttgcc cagggaaact gggactataa aatcgattca
     7141 ggaggagatg atgagatcgg aactttggtg gaatcgttca accagatgac ccgagagctg
     7201 aagcagagca atttagagct ggagcaaaga aagcgctttg tggagacgct tctcgccaat
     7261 attaccgcgg gcgtgatcgc tgtcgatccc acgggacgca ttacaacctg gaataagacc
     7321 gccgaggata tgtttggagt cgatgctgcg ggagtgctgg gaaaaaatca ggaggaggtc
     7381 tttcaggttg gaccgcttca agtcatgcgt gacatcctgg aaagcgtgaa aggccgagag
     7441 agcgtggagc gccaaatcaa gatctcttta acggaccaat tgctcaccgt ggtggtaacg
     7501 gctgcaacct tgcgcgatga tgaaggaaac accatgggcg tcatggtctt tctcgaggac
     7561 atcacccaga ttcaaaaggt gcagagaatg gaggcatggc gtgaggtcgc gcggcgtatc
     7621 gctcatgaga tcaagaatcc gctcacccct attcagttgt ccgccgagcg gctccgaaaa
     7681 cggtatgcta aattgctcga tggcaacggt gcgattttgg acaagtgtac ctcgactatc
     7741 atccagcagg tggacgagct gaagaatctc gtcaatgaat tttcccattt cgctcgtcta
     7801 ccttcggccc acctcaccag gaacgacctg aatgggatcg ttcaggaggc cctgtttctt
     7861 tttaaagaag gacacaacga tattcgtttc cagtttcgca acgaagagat cccgccgctc
     7921 gaattggacc gagagcagat caaacgcgtt attatcaatt tattggacaa cgcggtggcc
     7981 gctgtaagcg aaaatggcga aatcagaatc gccaccagtc atgaccgctc tcgaggagtc
     8041 gtctatttgg aggtcgcgga taacggctgc ggtgtggcgc cagaagttag aggcagaatt
     8101 ttcgagccct atttttccac caagaagaat ggaacgggcc tgggactcac catcgtgagc
     8161 cagatcatcg aagatcaccg gggctacatc cgggtgcgac ccaatgaacc ccggggtaca
     8221 aaatttacga tcgagtttcc ggtgcaaacg gagagcgatc ttgtccagaa tgagaagagg
     8281 accatgcatt cctaggagac gtaatgaaag aacggttggg acagaaggtc atcatgatcg
     8341 tcgacgacga ggacaaagtt cgtgagtcgg tccgagaagt cttggccgac gagggctatc
     8401 gcgttgtgga taccgccgac ggcactggtg tgctcgaatt gattgaacag gaaaagcccg
     8461 gactcgtgct gctggatatt tggatgcccc agattgacgg catcgatctg cttaagcaga
     8521 ttaagagcaa ggaaccggcg atcaacgtgg tgatgatttc gggccatgga aatattcata
     8581 ctgcagtgac ggcgaccaaa ttcggggcgt tcgatttcat cgagaagccg ctctctttgg
     8641 atgcgctgct gctaacggtg cagcgggctt taggcgatta ttcgccgggt cgctctaaag
     8701 aaatcaacgg caacggaggc agaaaatcga aaaaagggaa gcgctctccg acggcggcaa
     8761 gaccggaaac ctctagtccg tccatcaagc aacgcacgtt gaagaaaagt attgtgacat
     8821 cgggacaggg gctccattcg ggaatgaaga caggtcttat tcttcacccg ctgccgccta
     8881 atagcgggat tctattcact ggcctttcgg gtgatgtgca ggtgcccgcc cacctggacc
     8941 atgtcggatc gaccggatat gcaacctcgc tgcgcgccaa agggtttgcc gtggggacgg
     9001 tggaacattt gttggccgtg ctgcatagtt acggcattac aaatttgctg gtcaaggttc
     9061 aaggggagat tcccattctg gatggttctg ccattgagtt ctgtaacctc attgatgaag
     9121 cgggtgttcg ggaagaaagt gaggaagggt ccgaaatcgt ggttgaccga caatatcgaa
     9181 tcgggagcca agggggtgag tcgatcagca tcgagccttc ggacgttttc tcggtgcact
     9241 atatcttgaa ttatccaaag cctgtgggta ttcaagatta tgtctacaca cacaaagatt
     9301 ctgccgggtt caaggaagaa atcgcgccgg cgagaacctt cggctttctc agggacatcg
     9361 aggagctaca acaagcgggc ttggtaaacg gtggcaagct caacaactgc atcctcatcg
     9421 atgacgagaa aatcgttaat acggatctgc gttttcccaa cgagttggcg cgccacaaga
     9481 tcctcgatat tatgggtgac ttctacttgc tcggaaaacc ggttcgaggt gcaattatcg
     9541 ctcatatgac gggtcattcc gacaacattg ctctgttgcg agaactgcgt agcgggatgg
     9601 gtctttagca gtgatcggta accttgtgct ctgcgggccc tgacgacaag aatgcaccct
     9661 ttttgaacaa ccataggtat ggcctcctct gtcccttttc cgcgactctt tacggtgggt
     9721 gaagccaacg cgttgctgcc cgctgtgcgc gggttgacgg aacggatcca agagaatatt
     9781 cagcggctga aaaacaaaag caaaatagtc attcgcgatc agcagctcga tccaggggcc
     9841 gcggatttca tggaccgatt gcaggaaaat agtgaaattg ctcggctcgt gtcggaagtc
     9901 aaagatggag tagagcagat ccagagttac ggttgcatct gcaagggagc ggaacaaggt
     9961 ctggtcgatt tcccgtgcct gctgggagca gaagtggtct ttctgtgctg gcaacagggt
    10021 gaaccgacga tcagctattg gcaccggatc gaagagggct ttaccgggcg ccgacccttg
    10081 ctcgatgtcg agaaaacggt cctcgacaaa aaaacttctt accattaatc ggtcaatttt
    10141 acgtttcggt ttcctctact tttgctcgtt gcgttcaatc cgtttttagg ctgagaggtc
    10201 tgtgatcgcg cacaataaaa acgcgctgtc tcttcgggtc aaagaagaag cacagcgtct
    10261 gggttttgag ctggtcggaa tctctccggt aaaacttccg cctcatgagg agtcgtttgc
    10321 agagtggctg cggcagggct tctcgggaca gctggaatat atgaaacgga ccgaggagat
    10381 acgccgcaat ccccagcggc tcgttccctg ggcagtgtcc atcgtatcgg tcggaatgaa
    10441 ctattatacg ccgttttccc gcccggcagc gtcgccggag ccgaaaggct ggatttcccg
    10501 ttacgcctgg ggcgatgatt accactatct aatcaaggac aagttggagt ctctcctcga
    10561 cagcattcga cggatctgta acagtcccgt ggaaggcaag gcctttgtgg attcaggtcc
    10621 ggtcctggag cgggagtttg ccggcctcgc ggggataggc tggatcggca aaaacactca
    10681 tcttatttct cctcaaaacg gctcgtggtt ttttttggga gaactttttc tcagcatacc
    10741 actcgcctac gatcggccga ttcgcgatcg ctgtggaaag tgcgatcttt gtttgaaagc
    10801 ctgccccacg ggagctttcg tgggacccta cgtgttggat gcgcgacgct gcatttccta
    10861 tctgacgatc gagctcaagg gctttgttcc tcttcagctc cgtccgctaa tcggaaatca
    10921 tatctttggc tgtgacatct gtcaggagat ctgtccctat aacgtaaagg ctcgcccgac
    10981 cacggaaagg gcatatgccc caagagaagg gcttcatgca cccgatctga ttccattgct
    11041 gtccctggat gaggaggagt ttcggcgccg gtttcgcggc agcccgatcc ttcgcgcgaa
    11101 gcgaaaggga ctgttgcgca atgttgcggt ggctctcggc aacgctagga gtcatgaggc
    11161 gctgccggca ttgatccggg cgttggatga tgaagaaact ctggtgcggg gtcacgtagc
    11221 ttgggcattg ggtcaaatcg gtttgccaaa gggaattagc gccttacaaa aaaggttgcg
    11281 catcgagtcc gatccgacgg tcaaaatgga aattgaagat gcgatcacgg aggcaatgaa
    11341 gccccttaat acggaaaccg gtagcacagc ttgagcgtaa attaatgatc gacgttcggt
    11401 gatgaaggtt acagtgccca tctccatcac ggcaccgact ttggctaatg ttcggttgat
    11461 tcggttggag tgaccaaggc tctagctaac cgctgtgcca agttcatggg gatgcgggtg
    11521 attcgtccat cagcatcgac gcaggcgtgc ctagttgatc ccgacgccaa ggacgcgctg
    11581 tcggcttccc gcgagatggt atagtcgaaa ttcagcgtcg cgcgcccgac tgaggtcagt
    11641 tgagttgcaa tcgtgatcac gtcgtcgtag cgagctgatc tggagtagcg acaggaaact
    11701 tcggtcacgg gaaaatgaag cccttgttct tcgatcgtct tatagggaag atcgatttgg
    11761 cgcagaaatt ctgatcggcc cttttcaaac caccgaagat aattcgcgta ataaaccaca
    11821 cccatctggt cggtgtctcc atagatgacg cgataggaag tgacgttttt tgtccttgcg
    11881 cggggttgca ccatgagcca atactcctag ttctttttcg ctcgtttctc aatgccgctt
    11941 agcgaagcga tctccgcctt gacaaggcaa agtgatgtat ggtttaagat ttcaaatttg
    12001 cgttacgaat tctcgacgta agcggtccaa atcgaacgcc tatctctcta agcaagaagg
    12061 aggtcaaagg atgaagggtt cagccaacta catgatcggg tcagtcacgg cattgctatt
    12121 ggcggtttgg atggtggctt gcacttcggt ccaggagcag ccaaaaccgg cagcgcctac
    12181 agtagcgcag caaccggtgc cggctaaaca ggaagaagcc aaagcgccga ccaaacaaga
    12241 gtccggcaag gcggagacaa aaacggccaa gacgaaagcc gtggaggtgg agggtgtgat
    12301 cagtagcgtc gggcaacgga cgattacttt tcaacttgaa cgaaaaggga aagtcaggga
    12361 agaagttgtg ggcgttgatg acaagactaa tattgaaaaa gacggcagca aagttaaatt
    12421 agggcagcta caagagacgg acaaagccct aatcaactat gaacccgatg cttacacacc
    12481 agccaactca gtgaaggtga tcggcaaggg tcaaatacag aaagttggcg gagacgacta
    12541 ggatcgataa cgatccgatc ggtcaccggc gattatctcg agatcaaacg actgctcgtc
    12601 tcttaatatt ctaaacgaga ttcgctctcc tggacgcttt ttccacatct catggtaaag
    12661 ctcagatcta gagcgaatct cttttttttc cacaccgata ataatatcgc cttctttaag
    12721 accgtatctt tcggctggcc cgccggccac tattcctccg acgacgaggt gtccggccag
    12781 aggttgcgga taaaatccta gccagggccg ccgggggcgg ctgcggactt gtccatactg
    12841 cttcagttcc tgggcgttct gtagataata atcgatgggg attgccagag aaaatttacc
    12901 gacttcagtt agattcagcg agacgacgcc aatgatttca cctttgaagt tggccatcgt
    12961 gccgccgcca aaacctggat tgggcgctgt cagacgaata gtcctttcca gcatgtattc
    13021 ccaatggccg tcgtagccgt ccatggaact tacatagccg ccgctaaccc ggcgcgcggc
    13081 ctggccgcta ctagcgatga tgaccgcggg ctggcccaag gcaactgagt ctaatgccgc
    13141 aggcttgaga aaaggcagtc cgcgcgcggg aattttgacc agtgcaaggc cggtttcctg
    13201 gtcctgggca gccaacgctc cgggatgctg ctcgccgtcc ggtaatgtga cagtgatgga
    13261 cgctgccccg acggttacat agtggacggt tagaatgtaa ccgtccggat cgacgatggt
    13321 gcctgagccg cctcgctccg accccaggtt acgggtcgag gggtggttgt cgggaacggt
    13381 aacttgaatg tctgccgtag cgggtagaat cgaatggatc agaccaatat gaccgttcaa
    13441 atcactactc ctccagagct gggggccggt cccgcggctc cggtgcttca gcctcgtttg
    13501 gataaaatct accctgccaa ctgtctcgtt cctctagttc cttgtagatc gcattgtgta
    13561 cgccgatctt gttgatcgac cattccggag ctacggtaat cgcccggtat gtttcgcccg
    13621 tcaaacgatg cataaccatt tccggcgcca aacgctcgag gaaatcaacc acgagtgtca
    13681 catattcgtt ctggctgaag agggtgaagc cacccgtggc ataaagtttt tccaggaccg
    13741 tattcttgat gatatgaaga ttgtgcagtt ttag
//
LOCUS       DASSWZ010000017         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_991028, whole genome shotgun sequence.
ACCESSION   DASSWZ010000017 DASSWZ010000000
VERSION     DASSWZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991028"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VFN58_00405"
     CDS             complement(<1..1642)
                     /locus_tag="VFN58_00405"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8916475.1"
                     /translation="MLGGHAVAQCLRNEGVRHVFSVPGESYIAVVDGLVDFPEIKLIT
                     NRQEGCAAFMAEGYAKATRKPGVCIVTRGPGATNASIAVHSAKYDSAPMVLLVGQVSR
                     AARGREAGQELDYGQFFGSIAKWVIEINDPKQIPRIVSRAFHIARTGRPGPVVVSLPR
                     DVIEQNAEISMIDPYPEVKPNPDPAAMEALIGRINAARKPVLLIGSGTQYTRAWQEVI
                     DFAEKFQVPVLTSYKRQDAFPNNHPNYAGNLLTSNKEAQRLAYEESDLLIVLGCRLNQ
                     QTTGAFTFPRPGRSFVQIDADEENIGQNARPEVGIIADAKQTLLAGLKHSGPRPNENR
                     AAWIAEQHAKQKDFCTPQDRPTPKVSMERIMKDIKSAMPADAITTTDAGSFGQWPQRY
                     IEFDHPDSYISPTLGCMGPGVPSAVAAKLAHPNRTVIAHVGDGGFLMTGQEMATAKQY
                     GANIITIVYNNEGYNSIRMHQEAQYPGRQYGVGLQNPDFAVMGESYGALGLKVTRDEE
                     FLPAFKKALAADRSALIEVQTDYEYVTPSATLSELAGKK"
     gene            1819..2862
                     /locus_tag="VFN58_00410"
     CDS             1819..2862
                     /locus_tag="VFN58_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916476.1"
                     /translation="MKILSLTILVILTMSWPAWSQGTKPKTLDELVAYSGADRQKIIL
                     EGAKAEGKVVWYTSLSGNYREIVDAFKKKYPEIQIEVYRAGSSDVAERLLSEAQAGRY
                     LADAMETTPGALMLLRDKGILKAFTSPELAKYPGEAKTNADGGRVYWVTDREAYLGFG
                     YNTKLIPSNQVPKNYQDLLRPEFKEKLAVTTESSSARVIGTMLKSKGEEYVKKLKDLD
                     IRLFKLASLGFLNLMMAGEIAGSPAVFQNQVIVAKEKGAPIDWVPLDLVPANAGGSAI
                     IANAPHPHAALLLTDFVIGADGQKLMEQFRYGVAWKQYPFKREYPERGMTTAQYQDAE
                     EKWSQLLRSLTRR"
     gene            2974..>3170
                     /locus_tag="VFN58_00415"
     CDS             2974..>3170
                     /locus_tag="VFN58_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916477.1"
                     /translation="MVFVLGYVGFLAFLFEGSARLAFSIPQIGKRLEANEEYTYIRNW
                     VREHQKAGTATYYSFDRYDPS"
BASE COUNT          702 a          843 c          885 g          740 t
ORIGIN      
        1 atttctttcc tgccagctcg gacaacgttg ccgagggcgt cacgtattcg tagtccgttt
       61 gcacttcgat caacgccgag cgatcggcgg ccagcgcttt tttaaacgcc ggcaaaaatt
      121 cctcgtcgcg cgtgactttc aagcccagcg cgccgtagga ttcgcccatg acggcgaagt
      181 cggggttctg taggccgacg ccgtattgac ggccaggata ctgggcctcc tgatgcatgc
      241 gaatcgaatt ataaccctca ttgttgtaga cgattgtaat tatgttcgcg ccgtattgtt
      301 tggctgtggc catttcttgc cctgtcataa gaaatccacc atcgcccacg tgggcgatga
      361 ccgtgcgatt cggatgcgct aacttggcgg ccaccgcgga aggcacgccg ggacccatgc
      421 agccgagggt cggcgaaatg taagagtccg gatgatcaaa ctcgatgtag cgctgcggcc
      481 attggccaaa gctgccggca tcggtcgtcg tgatcgcatc ggcgggcatg gcagatttga
      541 tgtccttcat gatccgttcc atggagactt tgggtgtggg acgatcttgc ggtgtgcaaa
      601 aatctttttg tttagcatgt tgctcggcaa tccacgcagc gcgattctcg ttgggccgcg
      661 gccctgagtg ctttaacccc gcgagcaatg tctgtttggc gtcggcgata atgcctactt
      721 ccgggcgcgc gttttggccg atgttctcct cgtccgcgtc gatctgcaca aacgaccggc
      781 ccggccgcgg gaaagtgaag gcgcccgtag tctgctggtt gaggcggcag ccgaggacaa
      841 tcaatagatc actttcctca taggcaagcc tttgcgcctc cttgttcgat gtcaaaagat
      901 tgccggcgta gttgggatga ttattcggaa aggcatcctg ccgtttatag gaagtcagca
      961 ccgggacctg aaatttttcc gcaaagtcga tcacctcttg ccatgcccga gtgtattgtg
     1021 tccccgagcc gatcaggagc acgggcttgc gtgccgcgtt gatgcgcccg attaaggctt
     1081 ccatcgccgc tggatcggga ttgggtttta cttcggggta cggatcgatc atggatattt
     1141 ccgcgttctg ttcgatcaca tcgcgcggca gcgacacaac caccggtccg ggccggcccg
     1201 tgcgcgcgat gtgaaacgcg cggctcacaa tccgtggaat ctgtttagga tcgttaatct
     1261 cgattaccca cttggcgatg ctgccaaaga attgaccgta gtccaactcc tggcccgctt
     1321 cacgaccccg cgcggcccgg ctgacctgtc cgaccaggag caccatcggt gcggaatcgt
     1381 atttggcgct atgaacggcg atgctcgcat tggtcgcgcc cggaccacgg gtgacaatgc
     1441 agacgccggg tttgcgcgtg gccttggcgt aaccctccgc catgaatgcg gcgcagccct
     1501 cttggcggtt ggtaataagt ttgatttcag gaaagtctac cagcccatcc acgactgcga
     1561 tgtagctttc gccaggcacg ctaaagacgt gtctcacccc ttcattacgc aggcactgag
     1621 cgactgcatg acctccaagc attgattgcc tcccgtgact tcgaatttct cggtacgtca
     1681 tagcgcctgt ctatggccgc tgtcaatgta tcatcatgct tttcagaacc cctggattga
     1741 tactgacgca cggagtgtga tagttcccga acgacgatcc gagcgatgct caaattcgtt
     1801 caacaaagaa ggagcaacat gaagatcttg agcttaacca ttttagtaat tctgacgatg
     1861 tcgtggccgg cttggagcca ggggaccaag ccgaaaacct tggatgagtt ggtggcgtac
     1921 agcggtgccg atcgtcaaaa gataattctc gagggtgcca aagcggaagg aaaagtcgtt
     1981 tggtatacct cgttgtcggg taattacaga gaaatcgtcg atgcgttcaa gaagaaatat
     2041 cctgaaattc aaatcgaagt ttatcgcgcc ggcagtagcg acgtggctga aagactactc
     2101 agcgaagcgc aagctggacg ctacttggcg gacgccatgg aaaccactcc gggagcgttg
     2161 atgctgttgc gcgataaggg cattctcaag gcctttacgt cgcctgaact ggcgaagtat
     2221 ccgggagaag ccaaaacaaa tgcagacggc ggacgtgtct actgggttac cgatcgtgaa
     2281 gcttatttgg gctttggcta caacacgaaa ctgatccctt cgaaccaggt gccgaagaat
     2341 tatcaggacc tattgcggcc cgaattcaaa gaaaaacttg cggtgaccac cgagagttcg
     2401 agcgcccgag tcataggaac gatgttgaaa tctaagggcg aagagtatgt caaaaagctc
     2461 aaggatttgg atatcaggtt gttcaagctc gcgagtctcg gttttctcaa cctgatgatg
     2521 gccggagaga tcgccggctc gccagcggtt tttcagaacc aagtgattgt cgccaaagaa
     2581 aaaggcgctc cgatcgactg ggttcccttg gacttggtcc cggcaaacgc cggtggatcg
     2641 gccatcatag ctaacgcgcc ccatccccac gccgctttgt tgctcaccga ctttgtcatc
     2701 ggcgccgacg gccaaaagtt aatggaacag ttcagatacg gcgttgcgtg gaaacagtac
     2761 ccgtttaagc gcgagtatcc cgaacgcggc atgactaccg cgcagtatca ggatgccgaa
     2821 gaaaaatgga gccagctgct ccgttcgctc acacgccggt aatcgaactg tccgtagtct
     2881 cggtcaggtc tcacggggat ttcgccgacc aatattttta gatggccagg cttcattaac
     2941 atcaattgac gcacaatgag taggaaaaag agattggttt tcgttctcgg ctatgtcggt
     3001 tttttagcct tcctgtttga gggctctgcc aggctggctt tttcgatacc tcaaatcggc
     3061 aaacgtctgg aggctaatga ggaatatact tacattcgca actgggtacg ggaacaccag
     3121 aaagccggga cggcaaccta ttactctttt gacaggtacg atccttccaa
//
LOCUS       DASSWZ010000018         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1274031, whole genome shotgun sequence.
ACCESSION   DASSWZ010000018 DASSWZ010000000
VERSION     DASSWZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274031"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1716
                     /locus_tag="VFN58_00420"
     CDS             220..1716
                     /locus_tag="VFN58_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916478.1"
                     /translation="MSIAITSLLTSAGMPRLAVAQTRVSIGVTETMETYNPYGDSVAL
                     LYDLYSEVTGPFCSYNFAKGEYEGHLAQRWEIKDPNTWVFYLDKRYTFNDGSPITSAD
                     VVHSMSRVLAPDSKHTTLASPMLKAEAVDKYTVKLITKNPTAPLLDFICDRFIITSKA
                     AYEKYGAAEADRYHMLGGGPYARKEVVPGQRIVLVKRPDHHEARKNPQAPDEVVFRTM
                     REPEQRVTALLNNEIQIAQFIPPHLRKRVEASPHHKVVSGDSLELMFLAMQPKPPFDK
                     KEVRQAVCYAIDRDKIIATLLEGFASRLDGPIGPGQYGYDPNLQPRYGYNPEKAKQLL
                     SQAGYPNGVDVELQTPVGRYTLDKQITEAMVPMLNAVGIRTRLATPEWPTLWANVQAG
                     KVPFYYMGRGQVLDPSPPLHQYFRTGVTPRIGYSNPKVDEILFKEQQEFDPAKRKKYL
                     AQAMSLITEEAPACFMWRHKLLWGISEKVDYQPAPDAGIFGLHIKVRR"
BASE COUNT          610 a          716 c          671 g          537 t
ORIGIN      
        1 gggggagcaa cgcctagtcc acgtgtgata tggtccgcat aagcgtattt attaaggccg
       61 atttgctgga cgtcatcgat gccttcgtgt gaggctatca aggaaacgat tctttggaaa
      121 aacccggcgc ggctttacgg cattgcgtga acggagaaac tagccggtgg aaggttatca
      181 ttgggaggtt atatgatgcc gagaaactgg aagtccgttt tgtccatagc aatcacaagc
      241 ctactgacgt ccgccggcat gccgcgcttg gcggtcgccc agacacgcgt ttccatcggc
      301 gtgacagaga cgatggaaac gtacaacccc tacggcgata gcgttgctct cctctatgac
      361 ttgtacagcg aggtcaccgg gcccttctgc tcatacaatt tcgcgaaagg cgagtacgag
      421 ggccatctcg cgcagcgctg ggagatcaaa gaccccaaca cttgggtctt ttatctggac
      481 aaaagataca cattcaacga cggctcccca attacctcgg ccgatgtggt ccactccatg
      541 agtcgggttc tcgcccccga cagcaagcat acaaccttgg ccagcccgat gctcaaagcc
      601 gaagctgtgg acaaatatac ggtgaaattg atcaccaaga atcccacagc tccgcttctt
      661 gattttatct gcgatcgctt catcatcacg agcaaggccg cctacgagaa atacggcgcc
      721 gcggaggcgg acaggtatca catgctcggc ggcggcccct atgcccgcaa ggaagtggtg
      781 cccggccagc gcattgtttt ggtcaaaagg ccggaccatc acgaagcgag gaaaaatcct
      841 caagcccccg atgaagtcgt cttccggacg atgcgcgagc cggaacaaag ggtcaccgcg
      901 ctgctcaata acgagatcca gatcgcccag ttcatacctc cccatctccg taagcgcgtg
      961 gaggcttccc cccaccacaa agtcgtcagt ggtgactcgc ttgagctcat gtttctcgcc
     1021 atgcagccca agcctccctt cgacaaaaag gaggtacgcc aggcggtctg ctacgccatc
     1081 gatcgggaca agatcatcgc cacgctcttg gagggtttcg cttcacgttt ggatggcccg
     1141 atcggtccgg gtcaatacgg ctatgacccg aatctacagc cgaggtacgg atacaatccg
     1201 gaaaaggcaa aacagctcct gtcccaagcc ggttatccca acggcgtaga tgtcgagctg
     1261 caaacgccgg tggggcgcta tacccttgac aagcagatca ccgaggcgat ggtcccgatg
     1321 ctcaatgctg tcggtattcg caccaggctg gccacacccg aatggcccac gctttgggcc
     1381 aacgtccagg ctggcaaggt ccctttttat tacatgggaa gaggccaggt gctcgatccc
     1441 agtccgccgc ttcaccagta tttcaggact ggagtgacgc ctcgaatcgg ctattccaat
     1501 cccaaggtgg atgagatcct ctttaaagaa caacaggagt ttgatcctgc gaagcgtaag
     1561 aaatacctcg ctcaggcgat gagcttgatc accgaggaag ccccggcctg cttcatgtgg
     1621 cgtcataagc tcctctgggg catctcggag aaggtggact atcagccggc ccccgacgcg
     1681 gggattttcg gcctccatat caaggtccgc aggtaagaat aatttctcat taccttaact
     1741 ccagatgtcc agcttacctt ggaagaaagg agcgatagac aatcgcgcct ttgtcggtga
     1801 caggctgttg aaaaaggctc acctgcttcg ttgcgtgtcc gatggcctcg ctcgacgtac
     1861 aaaaagagta cgcctgcgct caaccattct cacgacgcct cgcatctaag accttttgaa
     1921 cagcctgtca gagtattaga gttggtaaga aatttacttg ctagaaatcc gaacgcggtt
     1981 ggcaggagtt tgtcgcgggt aagccataag ttggcggcag tccgacccaa ccctcccacg
     2041 ttctcatcct atcgttgccg tggacgtgaa taacgataag ctagcctttg ctcgcgcttc
     2101 ggtgggactc acacgatcaa cgcccgcgaa gttgacgcgg ttaaagctgt ccgcgagctg
     2161 accatcgccc aactctgacg acgaaaagcc ctgatccgta tcgtcggttg gcgactgaga
     2221 aaatggattg gcttatcagt tactcgcgaa tgagattccg gcggaggaga tgaggacgcc
     2281 gccaaatacg tcaaaaagtc atgcgtggcc ttcgcgatgt ccctagaccg tttgctttga
     2341 agtattgatc aaatacagca acggactata aagtatagta ctgaccttat gtgctacgta
     2401 aaaactttat gacgccgtgc acaagcgcgt gccgcgcgcg gcccttgcct atcacagcgg
     2461 ttcatccgca tggtgcttga acgggccagt agtgcaccaa aatcaagacc atatgtcgaa
     2521 cgaaaatcag cact
//
LOCUS       DASSWZ010000019         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1231590, whole genome shotgun sequence.
ACCESSION   DASSWZ010000019 DASSWZ010000000
VERSION     DASSWZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231590"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFN58_00425"
     CDS             complement(<1..264)
                     /locus_tag="VFN58_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET8916479.1"
                     /translation="MPTLREKIAQMFLVGVGAQELTSEERLAMEHYSFGGFILFGHNC
                     CDPAQILSLTRALWDTGKGLPPFIAIDQEGGRVHRLPKPFTHFP"
     gene            327..968
                     /locus_tag="VFN58_00430"
     CDS             327..968
                     /locus_tag="VFN58_00430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET8916480.1"
                     /translation="MTKSKYIVVEGPIGVGKTSLARLLANEFQARTVFEKVEENPFLP
                     KFYQARESYAFQNQTFFLLNRYQQQVDLSQQDLFNQNTIADYLFAKDKIFATLTLSNE
                     ELNLYQQIYALLNARVSKPDLVVYLQARPEVLYKRVKKRDKKYERGVTFEYLSEVAQA
                     YNQFFFHYDETPLLVVNTSEIDFVASSKDLADLIKEINNMGLGTQHYIPLGSR"
     gene            1089..1892
                     /gene="panB"
                     /locus_tag="VFN58_00435"
     CDS             1089..1892
                     /gene="panB"
                     /locus_tag="VFN58_00435"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET8916481.1"
                     /translation="MGEKITVPDIITKKCQGEKVTCLTAYDYSFARILDEAGIDILLV
                     GDSVGCVFQGQANTLPVTIEEMIYHTRAVVRGRKRALVVGDMPFLSYQVSREQAIKNA
                     GRLLQEGGAEGVKLEGGLAMQATIEAIVKIGIPVMGHVGLTPQSVHRFGGYKIQGKNK
                     EQREAVMGDALAVEDAGAFAVVLEGIPLDLAKQISERLTIPTIGIGAGPHCDGQVLVI
                     HDMLGLFDDFMPKFVKRYADIKEVMTGAVKSFIAEVRERKFPGEEHSFK"
     gene            1985..>2454
                     /locus_tag="VFN58_00440"
     CDS             1985..>2454
                     /locus_tag="VFN58_00440"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET8916482.1"
                     /translation="MQIIEHISEMQHWSEATRRDGRRIVFVPTMGFLHDAHLSLVRDG
                     KKRGDRLVVSIFVNPAQFSPNEDFAAYPRDFERDRGLLEREHADVLFHPSVREIYPEG
                     YQTHVDVGRLSALLCGAHRPGHFQGVATVVAKLFNIVRPHVAIFGEKDYQQLQL"
BASE COUNT          581 a          604 c          695 g          574 t
ORIGIN      
        1 aggaaagtgg gtgaacggct tgggtagccg atgaactctt cccccttcct gatcaatggc
       61 gatgaacggc ggcaggcctt tgccggtatc ccaaagagcc cgggtcaaag agagaatctg
      121 cgcgggatca cagcaattat ggccaaagag gatgaagcca ccgaaggaat agtgttccat
      181 cgccagccgt tcttcgctgg tcagttcctg agcgccgacg ccgaccagga acatctgggc
      241 gattttttct cttagcgttg gcatcaatcg ggtctataat actgcctaga caattttagg
      301 tccattatgt aaatttgccg cgtcagatga cgaagagcaa atatatagtc gtcgaagggc
      361 ccatcggcgt gggcaagacc agtttggcta gactcctcgc caacgaattt caggcccgga
      421 ccgtctttga gaaggtggag gaaaatccat ttcttccgaa gttttatcag gctcgcgaga
      481 gttacgcctt tcagaatcag accttctttc tgctcaatcg ctatcagcaa caagtggatc
      541 tcagccagca ggatctcttt aaccaaaata ccatcgctga ttacctattt gccaaggaca
      601 agatcttcgc gaccttgacc ttgagcaacg aagaactaaa cctttaccag caaatctacg
      661 cgcttttgaa tgctcgggtt tcgaagccgg accttgtggt ctatctccag gctcgacccg
      721 aggtgcttta caaaagggtc aaaaaaaggg ataaaaaata cgaacgtggc gtaaccttcg
      781 aatacctctc agaggtggct caggcgtaca atcagttttt cttccattac gatgagacgc
      841 cgctactggt ggtgaacacc tcggagattg atttcgttgc gagcagcaag gatctggctg
      901 acttgatcaa ggaaatcaat aatatgggtt tggggacgca gcattatata ccgcttggat
      961 ccagatagcc tggactgaga cggatgccaa taggcaatac ttgggtggtc gattcggttt
     1021 accactcgcc attgttggcc ttccggctca gtgcccggaa ggcttttctg tttttggagg
     1081 gtgaatcaat gggagagaag atcaccgtac ctgatatcat cacaaaaaag tgccagggcg
     1141 aaaaagtcac ctgcctcacc gcttacgatt attcatttgc gcggattctg gacgaggccg
     1201 gtatcgatat cctcttggtc ggcgactcgg tgggctgcgt ctttcaaggg caggcgaaca
     1261 ccctgccggt caccattgaa gagatgatct atcacacgcg agctgtagta cggggacgaa
     1321 agcgcgctct agtagtcggg gacatgccgt ttctctccta tcaagtaagc cgggaacagg
     1381 cgatcaagaa tgccggacgg ttgctgcaag agggtggcgc cgagggggtg aaactcgaag
     1441 gcggtctggc catgcaagct acgattgaag ccatcgtcaa aatcggcatc ccggtcatgg
     1501 gtcacgtcgg actcacgccg cagtcggtgc accgtttcgg cggctataag attcagggta
     1561 aaaacaaaga acagagggaa gcggtcatgg gggatgcttt ggcagtggag gatgccggcg
     1621 cgtttgcggt ggtgttggag ggtattcctc tagacttggc caagcagatc tccgagcggc
     1681 tgaccatccc aaccatcggc atcggcgcgg gcccgcattg tgacggtcag gtgctcgtga
     1741 tccatgacat gctagggttg ttcgatgatt ttatgccaaa gtttgtcaag cggtatgccg
     1801 atatcaaaga agtgatgacc ggggcggtca aaagtttcat tgctgaggtt cgcgagcgaa
     1861 aattccccgg cgaggagcat tcgttcaaat aacaatgagg agaacggagt gatggggatg
     1921 tgcggtaatc cttcattact ccaacactcc agtactccaa cattccaatt tagctgttct
     1981 gtccatgcag attattgagc atatctccga gatgcaacat tggagcgagg cgacacggcg
     2041 cgacggacgt cggattgttt ttgttccgac catgggtttt cttcacgacg cgcacctgag
     2101 tcttgtgcgc gatgggaaga agcgaggcga tcgcttggtt gtctccattt tcgtgaaccc
     2161 ggcgcagttc agtcctaacg aagactttgc tgcctatccg cgcgacttcg agcgggatcg
     2221 agggctgctg gaaagggagc acgccgatgt tctgtttcac ccttcggtcc gagagatcta
     2281 tccggagggc tatcagactc acgtcgatgt gggcagactg agcgcgctcc tgtgcggcgc
     2341 ccatcgaccc ggccacttcc agggagttgc cacggtggtc gcgaagctct ttaatatcgt
     2401 tcggccccat gtagcgatct ttggcgagaa agactatcag cagctgcagt tgag
//
LOCUS       DASSWZ010000020        12291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1075966, whole genome shotgun sequence.
ACCESSION   DASSWZ010000020 DASSWZ010000000
VERSION     DASSWZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075966"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VFN58_00445"
     CDS             complement(<1..1070)
                     /locus_tag="VFN58_00445"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET8916483.1"
                     /translation="MRERFDIAADLRKIARLLEIKGENPFKTQAYERGASAIENYQGD
                     FDALVKTGRLKEIPGIGTALAAIIDEIYHTGECWMVQQLLEGMPPGVIELSAVPGLNL
                     KKIIALHDALGIESIADLKTACQEGLVSKVKGFGLKSQAKLLADIESLQIPKDGSLLL
                     SHALETAERILKHLRAGPELIEAGLAGALRRRKETVRRISIVAASNQSREVLDRFLRF
                     PALAHTDELDETRCLARLAGGLRAELTVVAPKDYVAALHARTGSRRHIAKLQDLARSK
                     AMTLYPEPGSHTYRPSTDSENEIYRQLGIQYIPPEMREDEGEIEIASAGKLTQPVELA
                     DIRGMTHCHTIYSDGRNSIEEMA"
     gene            complement(1089..2480)
                     /gene="lpdA"
                     /locus_tag="VFN58_00450"
     CDS             complement(1089..2480)
                     /gene="lpdA"
                     /locus_tag="VFN58_00450"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET8916484.1"
                     /translation="MEQKQHDLVIIGAGPGGYVAAIRAAQLGLNVACVEKEQDLGGTC
                     LRIGCIPSKALLESSELFWRAKEDLAQHGIRAKDLQFDLPTIMARKEKTVKVLSKGVE
                     SLFRKNKISRYVGTGRIVEPGRVLVDTGNDAIDLKSRFILIATGSKPATLPHINLDGD
                     RVATSTEALSYKEVPGHLVVIGAGAVGLELGSVWKRLGAKVTILELMDRILPEMDRDI
                     ARDAMKLLEKQGLEFQLQSRVMEVRLEGNRCVVECEGKEPTLCDRVLVAVGRLPCTDG
                     LDLESLRIKTDKRGYIAIDKNYATSAGGIYAIGDVVVGPMLAHKAQEEGIACVESMVT
                     GYGHVDYHTIPNVVYTHPEVASVGKTEEDLKREGTDYRSGVFPFRANGRAQTLGQIEG
                     KVKVLADAKSDRILGVHILGARAGDLIAEAATAMNFSASSEDIARSCHAHPTLPEALR
                     EAALAVGGRALHS"
     gene            complement(2482..3741)
                     /gene="odhB"
                     /locus_tag="VFN58_00455"
     CDS             complement(2482..3741)
                     /gene="odhB"
                     /locus_tag="VFN58_00455"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET8916485.1"
                     /translation="MPLELKVPSVGESITEVEISEWQKAEGESVKRDEVVAILETEKV
                     TVELPAPGTGVITKILKKKGETAKVGDVIGYMEVDGKHQKELAEAKPQRDTEEKQESK
                     TSEAEKSKDATDRDRAETKDPVRLMPAARRILAERGLKPEEVPATGPGGRLLKEDALS
                     YLERVEESKAEQPTGEKKTLTSPDSDVREEEVVPMSPLRRRIAERLVEAQQTAALLTT
                     FNEIDMTAVMALRRQYRESFEKKYSIKLGFMSFFVKASIEALKLIPQVNAEVRGTDIV
                     YRNYYDIGIAIGGGRGLVVPVLRHAERMSFAEIERAIDDFARRAETNKLNLDELRGGT
                     FTITNGGIYGSLLSTPIINPPQSGILGMHAIQERPVAREGQIVIRPMMYVALTYDHRI
                     VDGREAVTFLRHIKGLLEDPARILLEV"
     gene            complement(3754..6558)
                     /locus_tag="VFN58_00460"
     CDS             complement(3754..6558)
                     /locus_tag="VFN58_00460"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET8916486.1"
                     /translation="MSEAERLPNTINLAFVEGLYLDYLKDPRSVPAEWRAYFDQLSKS
                     GRDNGSPRLGPSFRPPGIFNPSTVSSNDGFGAREAEVAAMQNRVDELIRNYRVRGHMI
                     AAIDPLGISRPRIPELEPEFYGFGEAEMTRSFSCDTLCDGAISLREILERLRNTYCRS
                     IGVQFMHIDELSVRHWLQERMERTQNRLELSREEKIRIFTRLTDAVIFEEFLRRKYLG
                     AKTFSLEGGESLIPLLDLAIDKAAEQEVQEIVIAMAHRGRLNVLANIIGKSPQEIFWE
                     FEDTSPELYIGRGDVKYHLGYSNDWMTASGRKVHLSLCFNPSHLEFVSPVALGRMRAK
                     QDRVGDSERKRGLVLLIHGDASFAAEGIAQETLNLSQLKGYAVGGALHVVVNNQIGFT
                     TSHAEARSSTYATDVAKMLQIPIFHVNGEDPEAVAQVVRLALDFRYQFKRDVVIDMYC
                     YRRLGHNEGDEPSFTQPVLYRAIEKRKSVRDSYLERLLELGGLTSGEAEKIAADGLKK
                     LEDDLSKARRPGYVPATDALRGIWSGYRGGLEPAGADIDTGVSKEKLAEFLDAQTRLP
                     EDFHPHPKIVRGIGIRREMAQGKRPLDWAAAESLAFASLACDGYRVRLSGQDSARGTF
                     SQRHAVLYDYENGQSYVPLQHVSSEQAAVEIYNSPLSEVGVLGFEYGYSLDCPDGLVL
                     WEAQFGDFVNAAQVIIDQFIASAEDKWHRLSSIVLLLPHGFEGMGAEHSSARLERFLQ
                     LAAEDNIQIVYPSTPAQYFHCLRRQMLSRWRKPLVVLTPKSLLRNPKSVSTLDECAVG
                     RFRRIIPDPLVERDQRVDKVLLCSGKIYYELEEERTRLGQKNIAIVRLEQLYPFPEPE
                     LKDALAMYPDARSVSWVQEEPENMGAWHYLHAKFCNQVFGRPFSGIFRPASGSPATGS
                     HASHKREQKELLQQALIGGI"
     gene            complement(6699..7211)
                     /locus_tag="VFN58_00465"
     CDS             complement(6699..7211)
                     /locus_tag="VFN58_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HET8916487.1"
                     /translation="MVDILQVLAVLLVAVAMSLALAHALELPGKMRLSKEAYYAMQPI
                     YYPGFTIGGFAEPAGLILTIILLFVTPLGTADFWLTLAALLGLIAMQATYWLLTHPVN
                     KFWLRGQNLSGLGSGFFSFGTSRSRTRNETRSTEWTDLRDQWEYSHVARAGLALASLT
                     LLVIAISDGS"
     gene            complement(7269..7421)
                     /locus_tag="VFN58_00470"
     CDS             complement(7269..7421)
                     /locus_tag="VFN58_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916488.1"
                     /translation="MIRKLASGEYRLYSRKKDPKTGKRRNLGTFDTRAAAEKHERAVQ
                     HFKRHN"
     gene            complement(7500..7856)
                     /locus_tag="VFN58_00475"
     CDS             complement(7500..7856)
                     /locus_tag="VFN58_00475"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET8916489.1"
                     /translation="MNPRQFFLIVLALGGVSALVAGRASRSFPEVQAASLPTGLGSPS
                     AANAKLEKAVKSMFASDEQVKSAKLSVRADVTKNEVTLSGTVESEAVRSRAVELAKTA
                     HVGVVVNDKMTVKPRR"
     gene            complement(7928..8200)
                     /locus_tag="VFN58_00480"
     CDS             complement(7928..8200)
                     /locus_tag="VFN58_00480"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HET8916490.1"
                     /translation="MAKPKQWVRNVKTVSTYPPKDVFTKDAQTIATTMAKKSVSPKGI
                     GSGIRMIQYFINRAGKSLSTARKRELEKAKKILQKRKTRKKGASSL"
     gene            complement(8200..8694)
                     /locus_tag="VFN58_00485"
     CDS             complement(8200..8694)
                     /locus_tag="VFN58_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET8916491.1"
                     /translation="MPEKQTLERVRKAKQKGKSVSTQAGEFVREEMHHIREGRHGARS
                     AKQAIAIGLSKARRAGVSLPPPEKGTVSEKTRRNAKRAYQAGQRRKPKPSRRRSKATE
                     LALKREPRSAASRKALSQQARAAARKRTRTERSASARRAARTKGRKGRSAAARKAART
                     RGER"
     gene            complement(8754..10502)
                     /locus_tag="VFN58_00490"
     CDS             complement(8754..10502)
                     /locus_tag="VFN58_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8916492.1"
                     /translation="MTSTAADILIDSLHDWGVEVIFGLPGDGINGIMESLRRRHEQIR
                     FIQVRHEEAAAFMACGYAKYSGKLGACLATSGPGGIHLLNGLYDAKLDGQPVLAITGH
                     HYHDLIDTHAQQDVNLDRLFADVAVYNTRVMGPAHVENVVDLACRTALGYRGVAHINF
                     PVDLQDQEVRERSKRNVANHTSDVLAHRARLPAENDIVRAAAVLNAGKKIAILCGQGA
                     LHATDELEEVAERLGAPIIKALLGKAAVPDMSLYTTGGIGLLGTKPSQEALEECDTLL
                     MVGTSFPYIEFLPKPGAARGVQIDVDPARIGLRYPVEVGLIGDSRRGLRALLPHLQRN
                     ENRSFLDHAQAGTKEWWRLMEERGSRQDRPMKPQVVAWELGKRLSDTAIVSSDSGTIA
                     TWFARQIPAKRGQMYSLSGNLATMANGLPYAIAAQIAYPERQCIAFVGDGGFSMLMAE
                     FATAVKYQLPIKVIIIKNNTLGMIKWEQMVFLGNPEFGCELQPIDFVAFAKACGGTGM
                     AIEDPVDCGRILDRALATPGPVIVEAIVDPFTPPMPAKITLDQAKKFAESLARGEPNR
                     SKIAWTVLSDKVRELI"
     gene            10998..11762
                     /locus_tag="VFN58_00495"
     CDS             10998..11762
                     /locus_tag="VFN58_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916493.1"
                     /translation="MKMQKTVLTIAIGGVLLSVPATIPSAVADDPKTTSNPSIYSDRD
                     RTKESKDEKEKLEQTLKTGEEKPFYRRELEKMGWQITSVNYDKPDYLEYEIVKDKSSY
                     EVQIDFDKNSRKATKVDVTANAWKTDATERALREGKKVDYPNKTTANPSRFSDRDRMK
                     SAKSQEEKLEQALKTGEEKDFYRRQLEKMGWKVTSVNYNKPDYVEYEIVKGDTSYEVQ
                     IDIDKNSNKATKVDVVTNVWKTDATKNALKQTQARR"
     gene            complement(11922..>12291)
                     /locus_tag="VFN58_00500"
     CDS             complement(11922..>12291)
                     /locus_tag="VFN58_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916494.1"
                     /translation="ERNMQRKLRLIRGRRVRKQSRTSEKTLRNRDRLNHAPDHAETAL
                     LLIDVINDLEFEGGDKLLSHALAMAGALAALKRRAKAHGIPVIYANDNFGRWRSDFPT
                     LVQQFRGWNYNRELRDDVTG"
BASE COUNT         2657 a         3474 c         3292 g         2868 t
ORIGIN      
        1 gccatctctt cgatgctgtt ccggccatcg gaataaattg tgtggcagtg cgtcattccg
       61 cgaatatcgg caagctctac cggctgcgtg agtttgccgg cgctggcgat ttcaatctca
      121 ccttcatctt cgcgcatttc cggcgggatg tactgaatgc cgagctgccg gtagatttcg
      181 ttttcgctgt cagtggatgg ccgataagtg tgactgcctg gctcgggata caacgtcatc
      241 gctttcgagc gcgctaaatc ttgcagtttg gcaatgtgac gccgcgagcc ggttcgagcg
      301 tgaagcgcgg caacatagtc ctttggcgcc acaacagtca attcggctcg cagaccgcct
      361 gccaagcgcg caaggcatcg ggtttcatcc agctcatcgg tatgcgccaa cgccgggaag
      421 cgcaggaatc gatcgagtac ctcccgcgat tgattgctgg cggcgacgat agatatccgc
      481 cgtaccgttt ctttgcgccg ccgcagcgct cccgcaagac cggcttcgat cagctccgga
      541 ccggcgcgta gatgcttgag aattcgctcc gctgtctcca gcgcgtgact gagcagcaga
      601 gagccatcct ttggaatctg cagagactca atatctgcaa ggagctttgc ctgtgacttc
      661 aagccgaaac ctttgacctt actgaccaga ccttcttggc aagcggtttt gagatcggcg
      721 atgctttcga tacccagcgc gtcatgcagt gcgatgattt ttttcagatt tagtccagga
      781 accgcgctca gctcgatcac gccgggcggc atgccttcca gcagctgttg caccatccaa
      841 cattcaccgg tgtggtagat ctcatcgata atcgccgcga gggccgtacc gatgccgggg
      901 atttccttga ggcgaccggt tttgacgagg gcgtcaaaat cgccctggta attttctatg
      961 gcactcgcgc cccgctcata ggcttgcgtc ttaaaaggat tctcgccttt tatttctaaa
     1021 agacgggcga tcttgcgcaa atcagcggcg atatcgaacc tttccctcat aaaccagcaa
     1081 ccgcggtatc aagaatggag cgcgcgtcct cccaccgcta gggctgcttc ccgcagcgct
     1141 tcgggtagag tgggatgagc atgacacgac cgtgcaatgt cctcgctact cgctgaaaaa
     1201 ttcatggcgg tggcggcctc ggcgatgaga tctcccgccc gtgccccaag gatgtgaacc
     1261 ccaagaatac gatcgctctt ggcatcggcc agcactttca ctttgccttc gatttggccc
     1321 aaggtctggg cgcggccatt ggcccggaag ggaaagaccc cgcttcgata atcagtcccc
     1381 tctcgcttga gatcctcctc agtttttccg acggacgcta cctccggatg ggtgtacacc
     1441 acgttgggaa tcgtgtgata atcgacatgg ccatagcctg tgaccatgct ttccacacaa
     1501 gcaatccctt cttcttgagc tttatgggcc agcatcggac cgacgacgac atcaccgatc
     1561 gcgtagatgc ctccggcgga cgtcgcatag tttttatcga tggcgatgta acctcttttg
     1621 tcggtcttaa tgcgcaaaga ttcaagatcc aacccgtccg tacatggcaa gcggccgacg
     1681 gcgaccagga cgcggtcgca gagcgtgggc tctttccctt cacactcgac cacgcaccgg
     1741 tttccttcta agcgaacctc catgacacgg ctttgcagtt gaaactccaa cccttgcttt
     1801 tcgagaagct tcatcgcgtc gcgcgcgatg tcgcggtcca tctccggcag aatgcggtcc
     1861 atcagttcaa ggatggtgac tttggctccg agtctcttcc acaccgagcc gagctcgagc
     1921 ccgacggcgc ctgcgccgat gacgacgaga tggcctggaa cttccttata gcttaaagct
     1981 tcggtactgg tcgcgacccg atcaccatcc agattgatat gcggtaaggt ggccggcttg
     2041 cttcccgtgg caataaggat gaaccggctc ttgagatcga tggcatcgtt tcccgtatcg
     2101 accagaaccc gtccaggctc gacaatccgg ccagttccca catagcgtga aatcttattc
     2161 ttgcgaaaca gcgactcgac acctttggag agaactttta ccgttttttc cttgcgggcc
     2221 atgatcgtgg gaaggtcgaa ctggaggtct ttagcgcgaa ttccatgttg ggcgagatcc
     2281 tcttttgctc tccagaagag ctcgctcgat tccaagagag ccttactcgg aatgcagccg
     2341 atgcgtaggc aggttccacc gagatcctgc tccttctcca cgcaggcgac attcaggccc
     2401 agttgcgcgg cgcggatagc agccacatat cctcccggac cggcgccaat gatgacgaga
     2461 tcgtgttgtt tttgttccat gtcaaacttc gagcagaatc cgcgccggat cctcgagcag
     2521 ccccttgatg tgccggagaa aagtgaccgc ttcgcggccg tcgacgatgc ggtggtcgta
     2581 agtcagcgcc acgtacatca tcggacgaat gacaatttgt ccttctcggg caacgggtct
     2641 ttcctgaatc gcgtgcatcc cgaggatgcc gctctgaggc gggttgatga tcggcgtcga
     2701 aagcagcgag ccgtagattc cgccgttggt aatcgtaaag gtgccgcccc gtagctcatc
     2761 gagattgagc ttgttggtct cggcccggcg cgcgaaatcg tcgatggcgc gctcgatctc
     2821 ggcgaagctc atgcgttcgg cgtggcggag caccggaacc accaggcctc tgccgccgcc
     2881 tatggcgatg ccgatgtcat agtagttgcg atagacgata tcggtgccgc gcacctcggc
     2941 gttgacctgc gggatgagct tgagcgcctc gatggaagcc ttgacgaaga acgacatgaa
     3001 gccgagcttg atagaatatt tcttctcaaa ggattcccgg tattgccggc gtagcgccat
     3061 gaccgcagtc atatcgatct cgttgaaagt ggtcagcaaa gctgccgttt gctgcgcctc
     3121 cacgagtcgc tcggcgatgc ggcggcggag cgggctcatc ggaacgactt cttcttcgcg
     3181 aacgtcggag tcggggctcg tgagagtttt tttctcgccg gtcggttgct cggcttttga
     3241 ttcttcaact ctctcaagat agctcaaagc gtcctctttc aggagacgac ctcccggacc
     3301 tgtggccggc acctcttcgg gcttaaggcc acgctcggcc agaattcgcc gcgccgccgg
     3361 catcagtcgc actggatcct tggtctctgc ccgatcccga tccgttgcgt ctttggattt
     3421 ctcggcttcg ctggtcttgc tctcttgttt ctcctcggta tctcgctgag gctttgcctc
     3481 agccagttcc ttctggtgtt ttccatcgac ttccatgtag ccaatcacat caccaacttt
     3541 agctgtctca ccctttttct ttagaatctt tgtgattaca ccggtgccgg gcgcgggaag
     3601 ctcaacggtc accttctcgg tctcgaggat cgccacgacc tcgtcccgct tcaccgactc
     3661 cccttctgcc ttctgccact cggaaatttc cacctcggtg atcgactcgc ccacgctggg
     3721 gactttgagt tctaacggca taaaaactta tccttaaata ccaccgatta gggcctgttg
     3781 gagaagctcc ttctgttcgc gcttgtggct cgcgtgagaa ccggtggctg ggcttccgga
     3841 cgcaggccgg aaaattcctg aaaaaggccg accgaaaacc tgattacaaa acttcgcgtg
     3901 aagataatgc caggcgccca tattctcggg ttcctcctga acccaggaga cactcctggc
     3961 gtctgggtac attgccaagg catccttgag ctccggctct ggaaaggggt agagctgttc
     4021 aagtctgacg atggcgatat ttttttggcc aagacgtgtg cgctcctcct cgagctcgta
     4081 gtagatcttg ccgctacaca aaagaacctt atccacccgt tgatcgcgct ctacgagcgg
     4141 atccgggatg atacgccgga atcggcccac ggcacactcg tctagggtcg agaccgattt
     4201 cggattacgc agaaggctct tgggagtgag caccaccaga ggctttcgcc accggctcag
     4261 catctggcgt ctcagacaat gaaaatattg ggctggagtc gaaggataaa cgatctggat
     4321 gttgtcctcc gcggcgagct ggagaaaacg ctcaaggcgg gcgctcgaat gctcggctcc
     4381 cattccttcg aatccgtggg gcaaaagcaa aacaatcgaa ctcagccggt gccatttgtc
     4441 ctcagcgctg gcgatgaact gatcaatgat cacctgggcg gcgttgacga aatcgccaaa
     4501 ctgggcttcc cagagcacca ggccatccgg acaatcgaga ctgtaaccgt actcgaagcc
     4561 caaaactcct acttccgaga gcgggctgtt ataaatctct accgccgctt gttcagaaga
     4621 tacgtgctga agcggaacgt aagactgtcc gttctcgtag tcgtagagga cggcatgtct
     4681 ttggctgaag gtgccacggg cgctatcctg tccggagagg cgcacgcgat aaccgtcgca
     4741 agcaaggctg gcaaaggcca gagactcagc ggcagcccag tccagaggtc gtttgccctg
     4801 cgccatttcg cgcctgatcc caatcccccg tacgatcttg ggatgagggt gaaaatcttc
     4861 gggaagtcgc gtttgcgcgt cgagaaactc cgcaagtttc tccttggaca ccccggtatc
     4921 gatatcggct cccgcgggct caagcccgcc tctatatccg ctccaaatgc cgcgtaaggc
     4981 gtcggtcgcc ggaacataac ccgggcggcg cgccttggag agatcgtcct cgagtttttt
     5041 caaaccatcg gcggcgattt tttccgcttc gccgctcgtc aacccaccca actccagcag
     5101 acgttccaaa taactatcac gcaccgattt tcgtttttcg atggcgcgat aaagtacagg
     5161 ttgggtaaag gaaggctcat cgccttcgtt gtgtccgagg cgtcgataac aatacatgtc
     5221 gatgaccacg tcgcgcttga attggtaacg aaaatcgagg gcgaggcgaa ccacctgcgc
     5281 cactgcctcg gggtcttcgc cgttgacgtg gaaaatcgga atctggagca tcttggccac
     5341 atccgtggcg taggtgctgg atctcgcttc ggcatgagaa gtggtaaacc cgatctggtt
     5401 attcacgacg acgtgcaaag ctccgccgac ggcgtaacct tttagctggc tcaaattgag
     5461 cgtctcctgg gcgatcccct ctgcggcgaa gctcgcgtcg ccgtgtatta gcaaaaccaa
     5521 gccccgcttt ctttcgctat cgccgacgcg gtcctgtttg gcgcgcatgc ggccgagcgc
     5581 tacaggactg acaaactcga ggtggctggg attgaaacaa agcgacaggt gaactttgcg
     5641 accggacgcc gtcatccaat cgttgctgta gccgagatgg tattttacgt cgcctcggcc
     5701 gatgtagagt tccggcgagg tgtcctcgaa ttcccagaag atctcttgcg ggctcttgcc
     5761 gatgatgttg gccagcacat ttaatcgtcc gcgatgggcc atggcgataa caatctcttg
     5821 aacttcctgc tcggcggcct tgtcgatggc aaggtccagg agtgggatca agctttctcc
     5881 cccttccaga gagaacgtct tggccccgag gtactttcta cggagaaatt cctcgaagat
     5941 gaccgcgtcg gtgagccggg tgaagatgcg aatcttttcc tcgcggctga gttcgagtcg
     6001 attttgcgtc cgctccatac gctcttgcag ccagtgccgc accgacagtt cgtcaatgtg
     6061 catgaactgg accccgatcg accggcaata ggtgttgcgg agtcgctcga ggatttcccg
     6121 cagcgagatc gcaccatcgc agagcgtgtc gcaggaaaaa cttcgagtca tctccgcctc
     6181 gccgaagccg tagaattccg gttcgagctc agggatgcgg ggccgagata tccccaacgg
     6241 atcgatggcc gcaatcatat ggccccggac acgatagttg cggatgagct cgtcgacccg
     6301 attctgcatc gcggcaactt cagcctcacg agcgccaaag ccgtcgttgc tgcttacggt
     6361 agacggattg aagatgccgg gcggacggaa cgatgggcca agacgagggc ttccgttatc
     6421 tcggccagat ttcgacagct gatcgaaata ggccctccac tcggctggaa cggaacgtgg
     6481 gtccttaaga taatcgagat aaagtccttc aacaaaagcg agattgatag tattcggcaa
     6541 acgctccgct tcgctcatgg tcggtcctga tcgtcacgaa aatgcttttg gatcctacaa
     6601 aaatcctaac agttgtggga attttcgcta gagcccgaga cgatcaagga aaatacgagc
     6661 gcgctaaagc cgcgttccag gtgagatcca cgacactgct aagaaccatc cgagatcgcg
     6721 atcacgagta gggttagact tgcgagcgca aggcctgcac gtgctacgtg ggaatactcc
     6781 cattgatcac gcaagtccgt ccattccgtc gaacgggttt cgtttcgggt tcgagaccgg
     6841 ctggttccga atgagaaaaa gccggagcca agtccactta aattttgacc ccgaagccaa
     6901 aacttgttca ccggatgcgt cagcagccaa tacgttgcct gcatagcgat aagcccgagt
     6961 aatgctgcaa gggtcagcca gaaatccgcg gttcccaaag gcgtaacaaa caagaggata
     7021 atcgttaaaa tcaaaccggc gggctcagcg aagcctccaa tggtgaaccc cggataatag
     7081 atgggctgca tggcgtaata cgcttcctta ctgagccgca tctttccggg taactcaagg
     7141 gcatgtgcga gagccagcga catcgccacc gcgaccagta gcactgccag cacttgcaga
     7201 atatcgacca tttatatttc ccttgctcgg ctaggccgag aatctcctat ccgttcgaga
     7261 tgacaagact aattgtggcg tttgaaatgc tggactgccc gctcgtgttt ctcggccgca
     7321 gcgcgcgtgt cgaaggtacc caagttccgg cgcttaccgg tcttcggatc tttctttcgc
     7381 gaataaaggc gatactctcc agaggctagt ttgcgaatca tatcgggcag ctcctgtata
     7441 gggacaatgc taatgtcgcc cccgcaacgt cacgcgtgcg ctcccgactg cgtagttttt
     7501 catcgtcgcg gctttacggt catcttgtca ttaactacga cgccaacatg agcggttttt
     7561 gccagctcca cggccctcga tcgtacggct tccgattcca cggtaccaga cagcgtcact
     7621 tcatttttcg ttacatccgc tcgaacgctc agtttcgcgc tttttacttg ttcatcgctg
     7681 gcgaacatgg acttaactgc tttttcgagt ttggcatttg cggcgcttgg actgcccagc
     7741 ccggttggca aggatgccgc ttgaacttcg ggaaaagatc tggaagcgcg acccgctact
     7801 aaagcgctca ccccgcctag tgcgagtacg attaaaaaaa actgccttgg attcatgcca
     7861 gtatctcatc ccgcaattgc atcggaaccg gtcattttca acttggctct tgcggcaaat
     7921 tcagtggtca taagctgctc gccccttttt tgcgggtttt tcttttctgc aaaattttct
     7981 tcgctttctc caattctcgc tttcgagccg tcgatagact cttgccggcg cggttaatga
     8041 aatattggat catgcggatg ccggaaccga tgcctttagg actcacgctc ttcttcgcca
     8101 ttgtcgtggc tatcgtttgc gcgtctttcg tgaatacgtc tttgggtgga taggtggaaa
     8161 ccgtctttac gttgcggacc cattgtttcg gtttagccat caccgctctc ctctagtgcg
     8221 cgccgctttg cgagcagcgg cggaacgtcc tttgcggccc ttggttcgcg ccgctcgccg
     8281 tgccgaagcc gagcgttcgg ttcgtgtgcg ctttcgcgca gcggcacgcg cctgctgcga
     8341 gagcgctttc cttgacgccg cgctccgagg ctcgcgtttc agcgcgagct cggtggcttt
     8401 tgagcggcgg cgagatggct tgggtttgcg tctctgaccc gcctggtagg cacgcttggc
     8461 gttgcgccga gtcttctcgg aaaccgtacc cttttctggc ggtggaaggc tgacgccggc
     8521 acgccgtgct ttcgatagac ctatcgcgat cgcctgttta gcggaccgag cgccgtgtct
     8581 gccttccctg atgtgatgca tctcttcacg aacaaactcg ccggcctgcg tgctgacgga
     8641 ctttcctttc tgcttggctt ttctaactct ctccaaagtt tgcttctccg gcatgacttg
     8701 ctctccgatt tatcaagcat tgaactccgt tggttaatct tttcagccca ccgttaaata
     8761 agttctcgta ctttgtctga aagcacagtc caggcgattt ttgaacggtt cggttcacct
     8821 cgggctaggg attcggcaaa cttcttcgct tgatcaaggg taattttagc gggcatcggc
     8881 ggcgtaaacg ggtcaacgat ggcttccaca ataaccggtc caggggtcgc gagcgcccgg
     8941 tcgaggattc gaccgcaatc cacgggatcc tcgatcgcca tgccggtccc gccgcatgct
     9001 tttgcaaaag cgacgaaatc aatgggctgc agttcgcagc cgaattcagg attgccgaga
     9061 aagaccattt gctcccactt gatcattccc aacgtgttat tctttatgat gattaccttg
     9121 atgggaagct ggtatttgac ggcagtcgca aattccgcca ttagcatcga gaagccgccg
     9181 tcaccaacga acgcaatgca ttggcgctcc gggtatgcga tctgcgcggc gatcgcatac
     9241 ggcagtccat tagccatagt ggcgaggttt cccgacaagg aatacatctg cccgcgcttg
     9301 gctggaatct ggcgcgcaaa ccaggtggcg atggtgcctg aatccgaaga gacgatggcg
     9361 gtgtccgaga gtcgtttgcc gagctcccac gcgaccacct gaggcttcat gggccggtcc
     9421 tgcctgctgc cccgttcctc catcaatctc caccattcct tagttccggc ttgggcatgg
     9481 tcaaggaagc tgcgattttc gtttcgctgc aaatggggca aaagcgcgcg caggccgcgc
     9541 cggctgtcac cgataagacc cacttcaacg gggtagcgca aaccgatacg ggcgggatcc
     9601 acgtcgattt gaacgcctct tgccgccccc ggcttgggca agaactcgat ataaggaaac
     9661 gatgtcccta ccattagaag agtgtcgcat tcttcgaggg cctcttgcga tggcttggtg
     9721 cctagcagtc cgattccgcc tgtcgtgtac aggctcatat ctggaacagc cgccttgccg
     9781 agcaaagcct tgatgatcgg cgcgcctaat ctctcggcaa cttcttccag ctcgtcggtg
     9841 gcatgcaagg ctccttgccc gcaaagaatt gcgatcttct ttccggcatt caacactgcc
     9901 gcggctctga caatgtcatt ttccgccggg agccgtgccc gatgggcaag gacgtcggaa
     9961 gtatggttgg caacgttgcg ctttgagcgc tcgcggactt cctggtcttg cagatccacc
    10021 gggaaattaa tgtgcgccac gcctcgatag ccgagagccg tacggcacgc caggtccact
    10081 acgttttcca catgcgccgg ccccatcaca cgagtattat agacggccac gtcggcaaac
    10141 agccggtcga ggttcacatc ttgctgggca tgtgtgtcga tcagatcatg gtaatgatgc
    10201 cccgtaatgg caagaaccgg ttggccatcg agcttcgcat catacagacc gttcagcagg
    10261 tgaatcccgc cggggccgga tgtggccagg caagccccca gcttgccact gtatttagca
    10321 tagccgcagg ccataaaggc ggcggcctct tcgtgccgga cctgaatgaa acggatttgt
    10381 tcgtggcgtc ggcgcaggga ttccataatg ccattgatgc cgtcaccggg aagaccgaaa
    10441 attacctcga caccccaatc gtgtaagcta tcaatcagaa tatctgccgc agtgctggtc
    10501 attcgtctga ccttccctga tttctattct cttctggaac tggtgctgat catgcacgcc
    10561 tagaaaagtg caaaccgaat gccactattt ctgtgacgag tctccagaac cgctccgagg
    10621 ctcgcattcc aattactcgg cgcgtcgaaa cactgctgct ggtggtctgg cccttaatcg
    10681 aactgtattg aaagatcgtc aggatccaac tcaaataaga attttccgct tagcgagccc
    10741 aaagaattga tccccgatga taatcgtaaa actagactct gcccgtgtcg gtaaacaccg
    10801 gactacgtgt tagatttttc caaataagcg taacccaaaa atcttaccga agtttttctc
    10861 tagctcacaa tacttaaaat cccacgtttt ggttcaaatt acagcgcatg gcatatctgt
    10921 tgcgtattac aaagttttat taactatgtg cggcaaaaac taaccgaacc tcattcaaat
    10981 aaacgggagg aaatatcatg aaaatgcaaa aaacagttct gacgattgcc atcggtggag
    11041 tactgttatc ggttcccgct acgattccga gcgcggtcgc cgacgatccc aagactacat
    11101 ccaatcccag catttacagc gatcgcgacc gaaccaaaga gtcgaaagat gagaaagaaa
    11161 aactggagca gacgctgaag acgggggagg agaagccgtt ctatcgccgc gagctggaaa
    11221 aaatgggttg gcaaatcact tcggtgaatt atgacaagcc cgattatctc gagtatgaga
    11281 ttgtgaaaga caagtcctcg tacgaagtgc aaatcgattt cgacaagaat agccgtaagg
    11341 caactaaagt cgatgttacg gcaaatgcct ggaagacgga cgccactgaa cgcgctctaa
    11401 gggaagggaa aaaggtagac tatccaaaca agaccacagc caaccccagc cgcttcagcg
    11461 acagagatag aatgaagtcg gcaaagagcc aggaagagaa actggaacaa gcgttgaaga
    11521 cgggtgagga aaaggatttc tatcgccgcc agcttgaaaa gatgggttgg aaggttactt
    11581 cggtgaacta caataagccc gattacgtgg agtatgaaat cgtcaaaggc gacacgagct
    11641 acgaagttca aatcgatatc gacaaaaata gtaataaggc taccaaagtc gacgtagtca
    11701 cgaacgtatg gaagaccgac gcaactaaaa acgcacttaa acaaactcag gctcggcgct
    11761 aggttaaaat tgcaggatgt ttcggcgttt gcgccgcacg cgcgcaacgc ctaaaccgaa
    11821 aggagcagat tatgtttcaa ggatttattt tatggttgtt aggaataccc tttgggatca
    11881 ttgtgttact ttggttattt ggtatcgtcg gttaatatct atcaacctgt cacatcgtct
    11941 cggagctcgc gattgtagtt ccacccgcgg aactgctgca cgagcgtggg aaaatccgag
    12001 cgccaacggc cgaaattatc attcgcgtag atcactggaa taccgtgggc tttcgcccgc
    12061 cgcttaagcg cggccagagc gccagccatt gcgagcgcat gagataaaag tttatcgccg
    12121 ccttcgaatt ccaggtcatt aatcacatcg atcaacaaaa gggccgtctc cgcatggtca
    12181 ggggcatgat tcaagcgatc acgattcctg agtgttttct ccgaagtccg cgattgtttt
    12241 cggacgcgtc taccgcgaat taatcgaagc tttcgttgca tgttccgttc c
//
LOCUS       DASSWZ010000021         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1401477, whole genome shotgun sequence.
ACCESSION   DASSWZ010000021 DASSWZ010000000
VERSION     DASSWZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401477"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VFN58_00505"
     CDS             complement(<1..1340)
                     /locus_tag="VFN58_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET8916495.1"
                     /translation="MQDLFLIGSLPWWVIALVACGSVALLVQQFLSLKQRLSLGQSSF
                     LVLLRACVYGLLIFFLFGPALVEKRVAKLRRPLTVLVDSSQSMSFPADPKPTQDGQPV
                     KSRLDLVRDTLLAGKEPLIQELNRDYDLRLYRFGTALEPIAPASISQLKAQDQGTRLL
                     EVLQSAAREAGEQSGILLFSDGIANGDRKTLEETQSLPVPIFTIGVGDTEGFTDVRIA
                     DLRAPEFAFRGREVKLDLTVQAYGLKGKTFPLYFNRGKNLITSRSISVNADPFEQKIT
                     FSFTPKEIGTHSFSLAIPNQPGEQITQNNHKDFKIDVQRDKIRVLTLSGSPAWNYRFL
                     RMAMKQDPLIELVSFVFLRTPTDSVDVPENQLSLIPFPIDDIFLEELKNFDVVVFDDF
                     SHRAYFNPVYLDKVRDFVRDGGGLAMLGGARAFDSGGYADSSLKDVLPVELDGKG"
     gene            complement(1344..>2078)
                     /locus_tag="VFN58_00510"
     CDS             complement(1344..>2078)
                     /locus_tag="VFN58_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET8916496.1"
                     /translation="KGVFAAALAPRNPETSSKDKRPIQFVFAQLKYRGGDWNPHPLAV
                     TPLMDELMLRTSVEAATTRHEVAVTDGDLFNYPFLYIAGKYEFDPFTQDEIEILRRFL
                     SYGGFLLVDDALGQQGYGFDKALRREMQRIFPGNEFKRLPSGHAALRSYYLLRRIGGV
                     RIANPNLEGITIGNSTPIIYCQNDLGGAWERDQLGNWTNSCAPGGEEQRKDAFHLGIN
                     LIVYAMTENYKEDLIHVPFIRKRLTR"
BASE COUNT          469 a          545 c          615 g          449 t
ORIGIN      
        1 cctttgccgt cgagctccac cggaagcacg tccttcaggc tgctgtccgc ataaccgccg
       61 ctgtcgaaag cgcgcgcgcc gccgagcatt gccaagccgc cgccatcgcg gacgaaatcc
      121 ctgactttgt ccaaatagac gggattgaaa taagcgcggt gggagaagtc atcaaagacg
      181 accacgtcga aattcttcaa ctcctccaaa aaaatgtcgt cgatggggaa ggggatcaaa
      241 ctcaactgat tctccggcac atcgacactg tccgtcggag ttcgtagaaa tacgaaggag
      301 actagctcta tcaaagggtc ctgtttcatc gccatgcgca gaaatcgata gttccaagcg
      361 ggagagccgg acagagtgag cacgcgaatc ttatcgcgtt gcacgtcgat cttaaaatcc
      421 ttgtggttgt tttgggtaat ctgttctccc ggttgatttg gaatggcaag cgaaaagctg
      481 tgagtgccga tttcttttgg cgtaaagctg aaggtgattt tttgctcgaa ggggtcggca
      541 ttgacactga tagaacggct ggtgatcagg tttttaccgc gattgaaata cagagggaat
      601 gtcttgcctt tgagaccgta ggcctgaacc gtcaaatcga gtttcacctc gcgtccgcgg
      661 aaggcgaact ccggggcccg gagatcggcg atgcgaacat cggtgaagcc ctcggtatcg
      721 cccacgccga tggtaaagat aggcacgggc aaggactgag tttcttccaa agtcttccgg
      781 tcgccgttgg cgatgccgtc gctgaaaaga agaatgccgg actgttcccc ggcttcccgc
      841 gcggcggact gcagcacttc caaaagccgc gtcccttgat cttgagcttt cagttgcgag
      901 atggacgccg gtgcaatggg ctcaagggcg gtgccgaaac ggtagagacg taagtcataa
      961 tctcggttga gctcttggat caacggctct ttgcccgcta agagcgtatc tctgacgaga
     1021 tccaaccggc tctttacagg ttgcccgtct tgcgtcggtt tgggatcagc gggaaaagac
     1081 atgctttgcg agctgtccac cagaactgtg agtggtcgac gcagtttcgc cacgcgtttt
     1141 tccaccaagg cgggaccgaa cagaaagaaa atgagcaagc cataaacgca ggcacggaga
     1201 agaaccagaa acgaactctg tccaaggctc agccgctgct tgagcgaaag aaattgttgg
     1261 accagcaggg caacgctgcc gcaagcgacc agagcaatga cccaccaggg cagactgccg
     1321 atcagaaaaa ggtcctgcat ggttcaccgg gtgagccgct tgcggataaa cgggacgtgg
     1381 atcagatcct ctttgtagtt ttcggtcata gcgtagacaa tcagattgat gcccaagtgg
     1441 aaggcgtctt tgcgttgttc ctcgccgccc ggcgcgcacg agttggtcca gttgccgagt
     1501 tggtctcgct cccaggcgcc accgagatca ttctggcagt agatgatcgg cgtggaattg
     1561 ccgatggtaa ttccttccaa gttcggattg gcgattctta cgccgccgat gcgccggagc
     1621 aggtaataac tccggagcgc cgcgtggccg gagggcaggc gtttgaactc attgcccggg
     1681 aaaattcttt gcatttctcg ccgcagcgcc ttgtcgaagc catagccttg ctggccgaga
     1741 gcatcatcga cgagcagaaa gccgccgtag gagagaaagc ggcgcaggat ctcgatctcg
     1801 tcttgagtga aggggtcgaa ctcgtatttg ccggcgatgt aaagaaacgg ataattgaaa
     1861 agatcgccgt ccgtaacggc aacttcgtgt ctggtcgtgg cggcttcgac actcgtgcgc
     1921 aacatcagct cgtccatcag cggggtgact gccaatgggt gcgggttcca gtcgccgccg
     1981 cgatacttga gctgcgcgaa gacaaactgg atggggcgct tgtctttgga gctcgtctcg
     2041 gggtttcttg gcgctagtgc cgccgcgaaa actccttt
//
LOCUS       DASSWZ010000022         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_637467, whole genome shotgun sequence.
ACCESSION   DASSWZ010000022 DASSWZ010000000
VERSION     DASSWZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637467"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1119)
                     /locus_tag="VFN58_00515"
     CDS             complement(43..1119)
                     /locus_tag="VFN58_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916497.1"
                     /translation="MRSKTLFFSLFISSVIASVFPSLSFAQGAASALKQYEGLKGKER
                     EQRLSEGAKKEGRVVVYSFTAVDQLTPLLEEFQKRYPFIKAEHYRANATGVFNKFSTE
                     ARAGQTLADVIDISAGETHTLLQMGLIDPYLSPSREGIPKDFMDEKGYWTAHYHFVIA
                     LGVNTQHIKAAEAPKSYDELINPKWKGRLSLDPADQDLFGALLLHWGKEKALNYFRNL
                     AKLEPRMVSGHTQQANLVGAGEIHMAPWLYGYRPLQLKDEGAPVETLLFDPVLSNPAY
                     LLLTKNSPHPHAAALLIDWALSADGGMKFFAEKFGRTPTRTGLKERFPQLRVDKYLVV
                     KPEIVGPNFKEFTKLYNEIFGVGK"
     gene            1266..>2381
                     /locus_tag="VFN58_00520"
     CDS             1266..>2381
                     /locus_tag="VFN58_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8916498.1"
                     /translation="MAKQQRKEVAAGMSPTQILAEYIAGLSYRNIPHEVVAHIKLCII
                     DSVGCALFGSTLSWGKIITSFTKEMGKGKGALIWGDGAEVPSTNAPLANGTLIHSFEM
                     DDLHRVGVIHPGAEAIPAADALVRRAGKVDGKRFLTAMVAGYEVGCRVGMTGGASQLR
                     RGFHPSATSGTFAAGAAAAKMLQLDPVKTLHTLGIAGTQAAGLMAAQHSSMVKRMHPG
                     RSAQAGVYGALLANKGFTGIEDILEASYGGFCSTFCDNPNLSHLTQDLGERFETLNVG
                     CKPYPCCGSNHTSIDALKKILRDHPEVSAENVDKIRIRTTRATKLHVGWPYEPKSVTT
                     AQMNLPFCVAALLHDRDFFVDQVTEKSIRSDAVLQTTR"
BASE COUNT          545 a          661 c          632 g          543 t
ORIGIN      
        1 caagcggaac aacagctaaa caaggccggc tggaagccgt gattacttgc cgacgccaaa
       61 aatctcattg tagagtttgg tgaactcttt gaagttaggt cccacgatct caggctttac
      121 cacgagatac ttgtccaccc gtagttgagg gaaacgctcc ttcaatcccg tgcgcgtcgg
      181 cgttcggccg aacttttcgg caaaaaattt catgccgcca tccgccgaca gggcccagtc
      241 aatgaggaga gcggccgcgt gagggtgagg cgaatttttt gtcagtagaa gatacgcagg
      301 gttggacaat accgggtcga agagaagcgt ctccaccggc gctccctcgt ctttcaactg
      361 cagaggccgg tagccataga gccacggggc catgtggatc tctccggcac cgacgagatt
      421 ggcttgctgc gtatgtcccg aaaccattct aggttcgagc ttggcgaggt ttctaaaata
      481 gtttaaggcc ttttcttttc cccaatgaag caggagagcc ccgaacagat cctgatccgc
      541 cgggtcaaga gaaagtctcc ccttccattt cgggttgatc agttcgtcgt agctcttggg
      601 cgcttccgcc gctttgatgt gctgcgtatt gacaccaaga gcaatcacga aatgatagtg
      661 ggccgtccaa taccctttct cgtccatgaa gtctttggga attccttctc ggctgggcga
      721 tagataagga tcaatgagcc ccatctgcaa cagggtatgg gtctcacctg cggaaatgtc
      781 gatgacgtcg gctaaggtct gacccgcgcg tgcctcggtc gagaatttgt tgaacacccc
      841 cgtggcattg gcccggtagt gttctgcctt gataaatgga tatctctttt gaaactcctc
      901 aagcagaggg gttagctggt cgacggcggt aaagctgtaa accaccaccc gcccctcttt
      961 tttggcgcct tcactgagcc tttgctctcg ttctttgccc ttgagtccct cgtattgctt
     1021 aagggctgaa gccgcgccct gagcaaaact gagagacgga aataccgagg caatgaccga
     1081 agaaatgaaa agtgaaaaga acaaggtttt gcttctcata taacctccat ttagaaatgc
     1141 cttgacaacc gcgctggtat agtaggaaaa catttagtcg aagaatactg tcggtggaca
     1201 acgtcactcg ccttctacga gccgatgaaa aatcacagaa cttcacgcgc gtcaaggagt
     1261 tttttatggc gaaacaacag agaaaagaag ttgctgcagg aatgtctccg acgcagatac
     1321 tcgctgagta catagcggga ctctcttata gaaatatccc ccacgaggtg gtcgcgcaca
     1381 ttaagctctg catcatcgat tccgttggct gtgctctctt tggctcgact ttgtcctggg
     1441 gaaaaatcat tacttcgttt acgaaggaga tgggaaaagg aaagggagcg ctcatctggg
     1501 gcgatggcgc ggaggttccc agcaccaatg ccccgcttgc caacggtacg cttattcaca
     1561 gctttgaaat ggatgacctt caccgcgttg gcgtcattca ccccggcgcg gaagccatac
     1621 cggccgcaga cgcgctggtt cgacgcgcgg gcaaagtcga cggcaaacgc tttctcactg
     1681 ccatggtagc gggctatgaa gtaggttgcc gtgtcggcat gacgggcgga gcttcccagc
     1741 tccgccgagg atttcatccg agcgccactt ccggcacctt cgctgccgga gccgccgccg
     1801 ccaagatgct ccaattagat ccggtcaaga ctctccatac gctgggtatt gccggcactc
     1861 aagccgcggg tctcatggcg gcccagcact cctctatggt aaagcggatg catcccggcc
     1921 gctccgctca ggcaggcgtc tatggggctt tgctcgccaa taaaggcttt accggtattg
     1981 aagatatctt agaggcttct tatggaggct tctgctcgac tttctgcgac aatcccaacc
     2041 tgtcgcactt aacgcaggat ctaggggagc gatttgaaac tctcaacgtt ggctgtaaac
     2101 cttatccctg ctgcggcagc aatcacacct caatcgatgc cctcaaaaaa attctccgcg
     2161 atcacccgga ggtcagtgcc gagaacgtgg acaagatccg cattcgtacc acccgcgcta
     2221 ccaagcttca tgtcggttgg ccctatgagc cgaagagcgt gaccacggct cagatgaatc
     2281 tccccttctg cgtggccgct ctgctgcacg accgcgactt tttcgtcgac caggtcacgg
     2341 agaagtcgat tcgaagcgac gcggtactcc aaaccaccag g
//
LOCUS       DASSWZ010000023         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_538422, whole genome shotgun sequence.
ACCESSION   DASSWZ010000023 DASSWZ010000000
VERSION     DASSWZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538422"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFN58_00525"
     CDS             <1..504
                     /locus_tag="VFN58_00525"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET8916499.1"
                     /translation="SQQVTALYNGQELEMEKFPHQIAFNCIPHIDAFVDGGYTKEELK
                     MINETRKIFGEPSLRVTATTVRVPVFCGHSESVNVETEKKLTAADVKAILREAPGVIV
                     ADEPENNVYPMAIEATGKDATYVGRIREDDSVTNGLNLWVVADNLRKGAALNAVQIAE
                     ILIRDYL"
     gene            561..1292
                     /gene="truA"
                     /locus_tag="VFN58_00530"
     CDS             561..1292
                     /gene="truA"
                     /locus_tag="VFN58_00530"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET8916500.1"
                     /translation="MNIKLSVEYDGTNYLGWQMQPNGPTIQSVLEQAVSTFFGAPTRV
                     TGSGRTDAGVHALGQVVNFFCDREPDLHRLRRGLNALTPDDISIKGVEIVPDSFDARR
                     DGRSRTYEYRILNRPSPSPFYLNYVWHLHDPLDARRMRKAVRYLEGEHDFSCFRASGC
                     DAPHPVRTVYRSSLDQAGDLLVFTIEATAFLRHMARNIVGTLVEVGREQRTPNSFAEL
                     LEARDRTKAGPTAPPHGLFLMEVKY"
     gene            complement(1345..1710)
                     /locus_tag="VFN58_00535"
     CDS             complement(1345..1710)
                     /locus_tag="VFN58_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916501.1"
                     /translation="MAPATAAEALQKSAAVFRGRVVTIYRSFLDRVGITNTAGYRVQF
                     EITKHWKGTPSKSIVVITRITGEACGFPFEEKKEYLVYVVTEPKDIQTGICTGTKNIA
                     EAEQEMKELDKLLERSSHN"
     gene            2246..3769
                     /locus_tag="VFN58_00540"
     CDS             2246..3769
                     /locus_tag="VFN58_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET8916502.1"
                     /translation="MASSNPLSRIQHIVVLMLENRSFDSMLGFLYAEQGNKSPSGQPF
                     EGLTGKEANLDSTGKSIPVFKIKPTDKNAYFMPGTDPGEGYAATNSQLFGSVNAPTPP
                     VATNQGFVKDFSSTLGWEENTPSWAKQILPGTVAQNIMGIFTPEMLPVLSALARGYAV
                     CDHWYCSLPTETMPNRAFVCAATSQGHMDDKTKSFTVPSIFGLLGQNNVSWAIYGYDT
                     DPLTRHTFPDTSNADDSHFGLFKDFQAAAAGGTLPAYTFLEPSWSPTGNSQHPNYDVA
                     LGEQLIHDVYYAVYNGKAWEQTLLIITYDEHGGCYDHVAPPLGAVPPDNTPGEYGFDF
                     KRFGVRVPTIIVSPLIAPGTIFRVPAGTTPLDHTSILKTVEKRWGLPPLTARDAAAPD
                     IGDVLTLNTARTDDPLKGVKVPQASGKDPAKGQPSHLQQVHAELVAQLPVQDKHGNRI
                     PPAAELRTSAEYKAYIQSRTAAWKKSRKARTEFNTRSTKKRGAKKNTHATRKAKKKS"
     gene            3974..>4175
                     /locus_tag="VFN58_00545"
     CDS             3974..>4175
                     /locus_tag="VFN58_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916503.1"
                     /translation="MIDPIRRKILKAGAAATVMATAPRVFAQQTGQGGAAMSFYEKGP
                     VRIRYQEAGSGFPLLLIAGGGLN"
BASE COUNT          950 a         1192 c         1096 g          937 t
ORIGIN      
        1 agccagcaag tcaccgccct ttataacggg caggaactgg agatggagaa gtttccacac
       61 cagattgcct tcaattgtat tcctcacatc gacgcctttg tggacggcgg ttacaccaaa
      121 gaagaattaa agatgatcaa cgagacccgc aagattttcg gcgaaccctc gttgcgcgtt
      181 accgcgacca cggttcgtgt tccggttttt tgcggccatt cggagtcggt aaacgtcgag
      241 acagagaaaa agctgactgc ggcggatgtc aaagcgatat tgcgggaagc tcccggcgtc
      301 atagttgccg atgagccgga gaataacgtt tatccgatgg ccattgaggc cactggcaag
      361 gatgcaacct acgtggggcg aatccgcgag gacgattccg tgaccaacgg cctcaatctc
      421 tgggtcgtgg cggataattt gcgcaaaggc gcagctctca atgccgtcca gattgccgag
      481 attctgattc gcgattatct ctagccctct cgctgccgct ctcgagtcga aacaaaagac
      541 aacacgaaac aggttttttc atgaacatca aactcagcgt tgagtatgac ggcacgaatt
      601 accttggctg gcagatgcaa ccgaacgggc cgactattca atctgttctc gagcaggcgg
      661 tatcgacgtt tttcggcgca ccgacacgtg tgaccggttc cgggcgaacc gatgccggcg
      721 ttcatgcgct ggggcaggtg gtcaatttct tctgcgatcg agaaccggat ctgcatcgcc
      781 tacggcgcgg tctcaacgcg ttgaccccgg acgatatcag catcaaaggt gtcgagatcg
      841 tgcccgactc tttcgacgcg cgccgtgatg ggcgcagccg gacttacgaa taccgcatct
      901 tgaaccgccc cagtccttca ccgttttatt tgaactatgt ttggcatctg cacgatcccc
      961 tggatgcgcg gcgcatgcgg aaagccgtcc gttatctgga gggagagcat gatttttcct
     1021 gcttccgcgc ctccggttgc gacgcgccgc atccggtgag aaccgtttat cgatcctcgc
     1081 tggatcaagc tggggatttg ctggtcttta cgattgaggc cacggcgttc ttgcgccata
     1141 tggcgcgcaa tatcgtggga acgttggtcg aagtcggacg cgagcagcgc actcccaatt
     1201 cctttgccga attgctggaa gcccgagaca gaaccaaagc cggtccgacg gcgccacccc
     1261 atggattgtt tttgatggaa gtgaagtatt aggattcggc ggcaggctcg accaaaaata
     1321 acgggattgc ccgtaagggc tatgtcagtt gtgtgagctg cgctccagca gtttatccag
     1381 ttccttcatc tcctgttctg cttcggcaat gttctttgtc cctgtgcaga ttcccgtttg
     1441 gatatctttc ggctcagtca ctacgtacac cagatattcc ttcttttcct caaaggggaa
     1501 tccacaggcc tctccggtga tccgggtgat gacgacgatg ctcttcgatg gagttccttt
     1561 ccagtgcttt gtgatctcaa actggacccg gtagccggca gtattcgtga taccgacgcg
     1621 gtccaagaag gatcggtata ttgtgacgac tcgacccctg aagacagccg cagacttttg
     1681 caacgcttcc gcagccgtag ccggtgccat gcaagtgcac gcatacgccg gttctgtcag
     1741 cagaaaccaa gttccaacca gccatatcaa gaatccaatt ctcgtgtgtc ccatcaatat
     1801 tttttatcgg ctaccgacgg acagctcagt gctaagttta gcgtcgcggt tgcacaatac
     1861 aagttcgtcc aatcatgacg ttactaagct ttgcgcccaa caaatgcgct gttgggtacc
     1921 ctcgtcgctt cgctcctcag cgcccggtca tacagggcgt tcggcgacag ccaagaaaaa
     1981 tctcaagagt ataaagaata aatacaactc ttattgccga cttttacctg gttgtgcttg
     2041 tgtttgtgtt cgcgatcata ctagcatacg tccgactggc aggcggaact catgtccaac
     2101 acttagtcct tgtttacatt ggcatgggtg cctggtagta gtcacgcttt tctttcgcct
     2161 gtcgctacat tttccaaaac gagagttatc actcgcagtg cctcagaaat cagaattggt
     2221 caatgaactc ttctggagtt tcaatatggc tagtagcaat ccactctcta gaatccagca
     2281 catcgtcgtc ctgatgctgg aaaaccgatc ctttgactct atgctcggat ttctctatgc
     2341 cgaacaggga aacaaatcgc cctccggcca acctttcgag ggtctgacgg gcaaagaagc
     2401 caatctcgat tctaccggca agagcatccc cgtcttcaag atcaaaccga cagacaagaa
     2461 cgcttatttc atgcccggca ccgatcctgg cgagggctac gcggccacca actcccaact
     2521 ctttggcagt gtcaatgctc cgacgccgcc ggttgccacc aaccaggggt tcgtcaagga
     2581 cttctcttct acactcgggt gggaggaaaa cacgccaagc tgggcaaagc aaatcctgcc
     2641 gggaacggtc gctcagaata tcatgggcat cttcacccct gagatgctgc ccgtgctttc
     2701 agccctcgcg cgtggctatg ccgtgtgcga ccactggtat tgttccttac cgaccgaaac
     2761 catgcctaac cgcgcgttcg tttgtgcagc gaccagtcag ggccacatgg acgacaagac
     2821 caagtccttc accgtgccca gtattttcgg cctgcttggt caaaacaatg tgagttgggc
     2881 catctatggc tacgatacgg acccgctcac acgccatact ttccctgaca ccagcaatgc
     2941 agacgactca cattttggcc tcttcaaaga tttccaggcc gcagccgctg gtggaacgct
     3001 tccggcctac acgtttctgg agcctagctg gtcccccacc ggcaacagcc agcatcccaa
     3061 ttacgatgtc gctctcggcg agcagttgat ccacgatgtt tattacgccg tgtacaacgg
     3121 caaagcatgg gagcaaacgc tcctcatcat cacctatgac gagcatggtg gctgctacga
     3181 ccatgttgcg ccccctttag gtgccgtgcc cccggataac accccaggcg aatacggctt
     3241 cgacttcaag cggtttgggg ttcgtgtgcc cacgatcatt gtttctcccc tgatcgcgcc
     3301 aggcaccatt ttccgcgtac ctgcgggcac gacgcctcta gaccacacct ccatcttgaa
     3361 gaccgtggag aagcgatggg ggttgcctcc cctgaccgcg cgcgacgccg ctgcgccgga
     3421 tattggtgat gtgcttactc tcaacaccgc acgcacggat gatccgctca agggagtgaa
     3481 agttccccaa gctagtggca aggacccagc caagggccag ccttctcacc tacaacaagt
     3541 acatgcggaa ctggtagcgc agttgccggt ccaggacaag catggtaaca ggattccacc
     3601 ggcggctgag ttacgcacga gtgccgaata caaagcctat attcaatcgc gcaccgccgc
     3661 atggaagaaa agccggaagg cgaggacaga gttcaacacg cggagcacaa aaaagcgtgg
     3721 ggcgaaaaaa aacactcacg caacacggaa ggcaaagaaa aagtcctaag tctctggtgc
     3781 gcttatagaa gcgttccaag tgcagaagat tctgtggctt agatgcgatg ccgccgttgt
     3841 gggtaaggaa ggttgctacc taacaagcac atccacttga gccgcgcgag cgagtttcgt
     3901 atgattccct cattacaacg cgcgcggccc ggtgatgtgg agtgttagac ggccgaaagt
     3961 caggaggaca ccgatgatcg atccgatcag acgcaaaata ctaaaagcgg gcgccgcggc
     4021 gacggtgatg gccacagcgc cacgcgtgtt tgctcagcag actgggcagg gaggcgctgc
     4081 catgtccttc tacgaaaaag gccccgtccg cattcgctat caggaggccg gttccggctt
     4141 tcccttgtta ctcatcgccg gtgggggact aaact
//
LOCUS       DASSWZ010000024         7134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_495985, whole genome shotgun sequence.
ACCESSION   DASSWZ010000024 DASSWZ010000000
VERSION     DASSWZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495985"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFN58_00550"
     CDS             <1..982
                     /locus_tag="VFN58_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916504.1"
                     /translation="FVLLGPSGSGKTTLMRCIAGLVKPDAGNIVLDGKTVFSASPRNN
                     VPPEERQIGMVFQSYAIWPHMTVAENIGLVLTHGPARLSQSEARKRIRHALHLVQLDD
                     FEKRPARLLSGGQQQRVALARALAINPKLLLMDEPLSNLDARLREEVRTKIKKLVSEL
                     GITVLYVTHDQVEAMVLADRIAVMARGEILQVGNPYELYRDPKSPQVAEFFGSMNWLR
                     GKVLDKHCVETKIGRLEVDCRGETNGKVVVGIRPEDMKIDASQEILRNRLEGTMAHST
                     FVGDQMIFEIRINDTLLTAKAMPDGKRPEGKVSVYFPKEKMVVFPDASSE"
     gene            1164..1991
                     /locus_tag="VFN58_00555"
     CDS             1164..1991
                     /locus_tag="VFN58_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8916505.1"
                     /translation="MGTEAKPKGDVRHLDHFVVAVMDADRAEKFYTEILGARTLKRFD
                     SPNMNRVFMKLGENHVGLFSQGKTTLPKRESAASYPRHSFIVPEGEFEPMAEKIRRAS
                     PFVKDIQSEKGLGCCWNEGLVFQDSEGNFLEITKAKGISKTRVHHLHFDTTDLDASVS
                     FYKSILNFNLVEQKNGMAVIGVPSDQRVILNQVDELSEVTKTTYRGRHFAFTVSDDNF
                     HAIVDKLHRAGIDEHDDHGEREGRRPEQLGTYFREPSGFRLQITNEDSATFALHAND"
     gene            1984..3081
                     /locus_tag="VFN58_00560"
     CDS             1984..3081
                     /locus_tag="VFN58_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HET8916506.1"
                     /translation="MTEDQSVKNHSVSSASEPIREIDVLWITAGLGCDGDTIAITAAT
                     QPSLEDILLGALPGIPKVNFHNPFLSYENGENFLLPFHLAAEGKLAPFILVVEGSIPN
                     EKIKQEGYWAALGTDKTTGQPITTCEWIDRLAPNAWAVMAAGTCATYGGIHAMEGNPT
                     GAMGLADYLGWQWKSLASIPIVCVPGCPVQPDNFMETLLYLLYQAAGSAPMIPLDEAL
                     RPTWLFSQTVHEGCDRGGYYEQAEFTEEYGSRQCIVKLGCWGPVVQCNVGKRGWITGV
                     GGCPNVGGICIGCTMPGFPDKFMPFMNQPPGSILSTTAVMTYGKTIRALRNFTQASLN
                     KEPEWRHPTIQANEVQSSRFKVQGDKHSRSR"
     gene            3110..4498
                     /locus_tag="VFN58_00565"
     CDS             3110..4498
                     /locus_tag="VFN58_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET8916507.1"
                     /translation="MSQPAEAPLTPKEAESIIQGLAGVFFEASSKPSKNGGEPLQDDH
                     RPLPDIEARYHTLVEQVPAVVFMIFLDRGISEAYVSPYIEAALGFSQDEWLDDPIRWY
                     RQIHPDDKERWNVEAAQMVLSGEPLRSVYRIIARDGHTVWFHCDVKMVRTEEGRPWFV
                     HGIAFDITELKQAEEALHHAHDELEFRIQERTVELAKANSELQSEITERKRVQEDLLR
                     ARHELEMRVQERTAELAITNETLKSEIIERRRLEKSILEISEREKGRIAQDLHDGLGQ
                     HLTGVAFLSKVLERKLIEKSAPDACDAGKIARLVNEAINQTRELARGLLPVQSGSKGL
                     MSALQHLASEVEELFHISCHFKVDEEVLIRDIDVATHLYRIAQEAVTNAIKHGQSRRI
                     DIGLSHRRDKFLLSIQDDGLGFTERLENKEGMGIRTMNYRANMIGASLDIQKNSGGGT
                     VVICTLPLSMEE"
     gene            4510..5193
                     /locus_tag="VFN58_00570"
     CDS             4510..5193
                     /locus_tag="VFN58_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8916508.1"
                     /translation="MSSERKHPVKRKRTVFVIDDHPIVREGLARLINGEPDLFVCGVA
                     EDIYAALKSMEILKPDIAIADISLKGPDGIELIKHLKTRIPGLPVLVLSIHDESIYAP
                     RALRAGARGYIMKQEATENVLVALRRVLSGEVYLSDRMANKMLQQFVGAGGAGVKFPV
                     DRLSDRELEVFRLIGQGHGTRRIAEELHLSVKTVESYREHIKEKMTLGDASELVRYAI
                     QWMEQEKSQ"
     gene            complement(5364..5963)
                     /locus_tag="VFN58_00575"
     CDS             complement(5364..5963)
                     /locus_tag="VFN58_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET8916509.1"
                     /translation="MKGAPLPPITRSNTALVLHDMQNDFIKESLSKPEMQAVVKRTRA
                     LIDLAHSLQMPVIYTRVETDPAIGFPSERAPYLQRPGSTCVKGTPGAEIINDLKPRSD
                     DYVVTKVRSSAFYQTKLDTLLRVKGVWILVVAGGSTNWGVEWLARDARCRDLLTVALR
                     DCTYSATPEAQAASLANIDDFIGSVMNQDEVVQMLSKSK"
     gene            complement(6168..6695)
                     /locus_tag="VFN58_00580"
     CDS             complement(6168..6695)
                     /locus_tag="VFN58_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916510.1"
                     /translation="MPIAALCANISWEFIFSFLYPHDPPQNYITMVWFAFDLVIVFQT
                     LRFGRVEFEPAWLFYPAFILGLLVSFGAILAITVQFNDWDGKYAAFGQNLMMSVLFVA
                     MLLKRRNVKGQSIYIALFKMAGTLLPSILFFLRYPNSPLLNFLYGAIFVFDLIYTVLL
                     YTKHRELGINPWRRV"
BASE COUNT         1803 a         1787 c         1953 g         1591 t
ORIGIN      
        1 cttcgtcttg ctgggaccta gtggttcggg gaagaccacg ttgatgcgct gcatagcggg
       61 actggtgaaa cccgatgcag ggaatattgt tctcgacggc aaaaccgtct tttctgccag
      121 tccccgaaat aacgttcctc cggaggagcg gcaaatcggt atggtttttc agtcctacgc
      181 gatctggccg catatgacgg tcgccgaaaa catcgggctg gtgttgaccc atggtccggc
      241 gcggctttct caaagcgagg ccaggaaacg gatccggcac gctctccatc tagtgcaact
      301 cgacgacttc gaaaagcggc cggcgcggtt gctgagcggc ggccagcagc aacgggtggc
      361 tctggcgcgg gcattggcga tcaatcccaa gctgctgctg atggacgagc cgctgagcaa
      421 tctcgatgcg cggctcaggg aagaggtgcg cacgaagatc aagaaactgg tgagcgagct
      481 gggaattacg gttctctacg tgacccatga tcaggtggaa gccatggtgc tggccgatcg
      541 tatcgcggtc atggcgcgcg gagagatctt gcaagtgggg aatccttacg agctttaccg
      601 tgacccgaaa agccctcagg tggccgagtt cttcggctcg atgaactggc ttcgcggcaa
      661 ggtccttgat aaacattgcg tagagacgaa gatcggcagg ctcgaagtcg attgccgtgg
      721 cgaaacaaac gggaaggtcg ttgtggggat caggccggaa gatatgaaga tcgatgcgtc
      781 tcaagaaatc ttgcgcaacc ggctcgaggg aacgatggcg cattcgacat tcgtcggtga
      841 tcagatgatc ttcgaaattc ggatcaacga tacgctgcta acggcgaagg ccatgccgga
      901 cggcaagaga cctgagggaa aagtgtctgt ttattttccc aaggaaaaga tggtcgtgtt
      961 tcctgatgct tcctcggaat aggaatgaaa gtgcacccaa ttatagaatc taagcttgca
     1021 tttaacgtcc agaaagattc ctcgcttgcc gctcggaatg acaccttaag cgacttgtca
     1081 tcccgaagga agtgagggat cttgaaccaa tggaaccaca ggattcaaag gataagactg
     1141 attcaaaaaa ggaggatctg aacatgggca ctgaagcgaa acctaaaggg gatgtccggc
     1201 acttggatca ttttgttgtg gccgtgatgg atgcggaccg agccgagaag ttttacacgg
     1261 agattctcgg cgcaagaact ctgaagcggt tcgacagccc aaacatgaac cgggtattca
     1321 tgaaattggg cgaaaaccat gtcggactgt tttcccaggg aaaaaccact ttgcccaagc
     1381 gcgagagcgc cgcttcttat ccgcggcatt cctttatcgt tcctgaaggg gaatttgaac
     1441 cgatggcgga aaagatccgc cgcgcgagtc ccttcgtgaa agacattcaa agcgaaaagg
     1501 gattgggttg ttgttggaac gaaggattgg tcttccagga ctccgaggga aattttctgg
     1561 agatcaccaa ggccaaagga atcagcaaga cgcgcgtcca ccaccttcat ttcgacacca
     1621 cggatttgga cgccagcgtc agtttctaca agagtatctt gaattttaat ttagtcgagc
     1681 agaaaaacgg catggccgtg atcggagttc cctcggatca gcgcgttatt cttaaccagg
     1741 tcgacgagct tagcgaagtg acgaagacga cctatcgagg ccgccatttc gcctttaccg
     1801 taagcgacga taactttcac gccatcgtcg acaaactcca ccgagcgggg attgacgagc
     1861 acgacgatca cggggagcgc gaaggaaggc ggccggagca gttgggcact tactttaggg
     1921 aacccagcgg gtttcgcctg cagattacca acgaggactc ggcaacgttt gctctccacg
     1981 cgaatgactg aggatcagag cgtcaaaaat cacagcgttt cttcagcttc cgagccgatc
     2041 cgggagatcg atgttctatg gatcacggcc ggtctcgggt gtgacgggga tacgattgcc
     2101 atcaccgccg cgacccagcc gagtctggaa gatattttgc tcggggctct ccccggcatt
     2161 cccaaagtca attttcataa tccattcctc tcctatgaaa acggggagaa ttttttgcta
     2221 ccgtttcatc tcgccgccga aggtaagctt gcccctttca tcctagtggt tgaaggatcg
     2281 attcccaacg aaaagatcaa gcaagaaggt tactgggctg cattaggaac cgacaaaaca
     2341 accggccagc caatcaccac ctgtgagtgg attgaccgtc tcgcgcccaa cgcctgggcg
     2401 gtgatggcgg ccgggacatg cgccacctac ggcggcatcc atgcgatgga gggaaatccc
     2461 acgggcgcca tgggcctggc tgattacctg ggctggcaat ggaaatccct cgcgagtatt
     2521 cccatcgtgt gtgttcccgg ctgccccgtc cagccggaca atttcatgga gactttgctt
     2581 tatctcttgt accaggccgc tggtagtgca cccatgattc ctctcgatga agccctgcgg
     2641 cccacttggc tttttagcca gactgttcac gaaggttgcg atcgcggcgg atattatgag
     2701 caagccgagt tcacggaaga gtacggctcg cgccaatgca tcgttaaact gggatgctgg
     2761 ggacccgtgg ttcaatgtaa tgtcggaaag cgaggctgga tcactggagt gggcggctgt
     2821 cccaacgtgg ggggaatttg catcggctgc acgatgccgg gattcccgga taagttcatg
     2881 ccatttatga atcaaccgcc cggatcgatt ctttctacga ctgcggttat gacctacggc
     2941 aaaacgattc gcgcgctgcg caattttacc caggcgtcgt tgaacaaaga acctgaatgg
     3001 cggcatccga cgattcaagc caacgaggtt caaagttcca ggttcaaggt tcaaggagac
     3061 aaacattctc gttcacgttg aaccttgaac ttcgatagga cgaactcaag tgagtcagcc
     3121 tgctgaagca ccactgaccc cgaaggaggc cgaaagcatc atccaaggcc tcgcgggcgt
     3181 tttcttcgag gcgagttcca agccatccaa aaacgggggt gagccgctcc aagatgatca
     3241 tcgacccctg ccggacatcg aagccaggta ccatacctta gttgaacaag tccccgcggt
     3301 tgtcttcatg attttccttg atcgaggaat cagcgaggct tacgtcagtc cctacatcga
     3361 ggccgcactt ggattttcgc aggatgagtg gctcgacgat ccgatccgat ggtatcgcca
     3421 gatccacccc gatgataagg agcggtggaa cgtagaagcc gcccagatgg tgctctcggg
     3481 tgaacccttg cgatccgtgt atcggattat cgcgcgggac ggacataccg tttggttcca
     3541 ttgtgacgtc aagatggtcc gcactgagga gggtcgtccc tggttcgttc atggcattgc
     3601 atttgatatc accgagctca aacaagcgga ggaagcccta caccatgctc acgatgagtt
     3661 ggagttcagg attcaagaaa gaacggtgga acttgccaag gcaaattccg aactccagtc
     3721 tgagatcacc gagcgtaagc gggtccaaga ggatctgctt agagcgcgtc atgaattgga
     3781 gatgcgcgtt caagaacgga cggctgaatt ggcgattacc aacgagacct taaagagcga
     3841 gatcatcgag cgaagacgcc tagagaaatc gatcctggaa attagcgaga gagaaaaagg
     3901 gcgtatcgca caggatctgc atgacggttt aggacaacat ctgacggggg tcgccttctt
     3961 gagcaaggtg ctggaacgca agctgattga aaaatcggcc ccggatgcct gcgatgccgg
     4021 caaaatagcg cggttggtca acgaagccat taatcagact cgcgagctag cgcgaggatt
     4081 gctccctgtt caatcgggat caaaggggtt aatgtcggca ctccaacatc tggcatccga
     4141 agtcgaagag ctttttcata tctcgtgtca tttcaaagtg gatgaggaag tgttgattcg
     4201 cgacattgat gtggcaaccc acctctatcg aatcgctcaa gaggcggtga ccaatgccat
     4261 caaacatggt caatcccggc gaattgatat tggtctctct caccgccggg acaaatttct
     4321 cctcagtatc caagatgatg ggttgggatt cacggagcgg cttgagaaca aggaaggcat
     4381 ggggatacgt acgatgaact atcgcgcaaa tatgattggg gcatcgctgg acattcagaa
     4441 gaattcgggt ggtggaacgg tcgtcatctg cacgctccca ttgagtatgg aagagtaagc
     4501 gaggcatttt tgtcgagcga gcggaaacac ccggtcaaga ggaagcgcac cgtctttgtc
     4561 atcgatgacc atccgattgt gcgcgaagga ctggcccggt tgatcaatgg ggagccggat
     4621 ctttttgttt gtggcgtggc ggaagacatc tatgcagcgc tcaaatcaat ggagatcttg
     4681 aagccggata ttgccattgc ggatatttcc ctgaagggcc ccgatggcat cgagctgatc
     4741 aagcacttaa agacccgaat acccggtttg ccggtgctgg ttctctcaat ccatgacgaa
     4801 tccatctacg ccccgcgggc gctgcgcgcc ggcgctcgag gctatatcat gaagcaagaa
     4861 gcgaccgaga atgtgctggt ggcgctgcgt cgagtcctaa gcggggaagt ctatctgagt
     4921 gatcgaatgg ccaacaagat gctgcaacaa tttgttggag ccggcggagc gggcgtcaag
     4981 tttcccgtcg atcggctcag tgaccgcgag ctggaggttt ttcggctcat cggccaaggt
     5041 catggaaccc gccgcattgc agaagagttg cacctgagcg taaaaaccgt cgagtcttat
     5101 cgcgagcaca tcaaagagaa aatgaccttg ggtgacgcca gcgagcttgt ccggtacgcg
     5161 attcaatgga tggagcagga aaagtcccag tgacctaagg cctcgccatt tacgcccgcc
     5221 agcacgcaaa actccattgt caattctgat ttcaccgatt gagcctgtgg gaaggtgccg
     5281 ccgaacagga tatcattgat ggccgatccg ctagccccaa cagaaagggc caaaggtagg
     5341 tgtcagacac ctgcttgtca atttcatttt gacttggaaa gcatctgcac aacctcgtct
     5401 tggttcatta cactaccaat gaagtcgtca atattagcga ggcttgcagc ttgggcttcc
     5461 ggtgtcgccg aataggtaca gtctctcaat gccacggtta gtagatctcg acacctagca
     5521 tccctcgcga gccactcgac accccaattg gtagaaccgc cggccactac caaaatccaa
     5581 actcccttga ccctcaggag agtgtccagc ttggtttggt aaaacgcgct ggatctgact
     5641 tttgtgacta cgtagtcgtc gctccgaggc ttgaggtcgt ttatgatttc cgcgccggga
     5701 gtgcccttaa cacaggtcga accaggtcgt tgtaggtagg gagcgcgttc gctcggaaag
     5761 ccaatggctg gatcagtctc aactctcgtg tagatgacag gcatctgcag cgaatgggca
     5821 agatcaataa gagccctggt ccgtttcaca actgcctgca tttccggttt cgacagcgac
     5881 tcttttatga agtcgttttg catgtcatgc aataccaaag cggtattgga tcgagtgatc
     5941 ggaggcaaag gtgcgccttt catctcaatt cctttcagag tttgtaagaa ttactttgac
     6001 ccatgagtta acatcgcgcc tttttcattt ttcatgcggt gttcgtctcg acatagcgag
     6061 cactcatatc aaaaatgaat aaatcggaac aggccgtgtt gccaaaatct ttcagagaac
     6121 aattgttttg tgaaagtcct cagtcgggaa cgcctgctca tattcatcta aactctcctc
     6181 caagggttaa tccctaactc cctgtgtttt gtgtatagca aaaccgtata gatgagatcg
     6241 aagacaaata tggcaccgta aagaaagttc aaaagtggcg agttagggta gcgaagaaaa
     6301 aacaggatcg acggcaatag cgtcccggcc atcttaaaaa gtgctatata aatggactga
     6361 cctttaacgt ttcgacgctt cagcagcatc gccacaaaca aaaccgacat catgaggttt
     6421 tgcccaaagg ccgcgtactt accatcccag tcgttaaact gaaccgtgat cgccaggata
     6481 gcgccaaagc ttacaagcaa acccaaaatg aatgccggat agaatagcca cgccggctca
     6541 aactcaactc ttccgaaccg cagggtttga aaaacaatga ccagatcaaa cgcgaaccaa
     6601 accatagtaa tgtaattctg cggtggatcg tgaggataga ggaaagaaaa tataaactcc
     6661 caagagatat tggcacaaag ggccgctatc ggcataccat aagttttatc ccgaaaacct
     6721 aatcggatga ttaatatata gaccgcggtc caaaaaactc cggatccgat cttcagactc
     6781 cagcttagtt caggagagac tgtcattcaa aaccaatgcg ccagttggtg atcgcataga
     6841 gagcactttc ccagtgggca gggcggcaag acgaaagaga agggaacccc gtcagccctc
     6901 ttgaccttag ttgtgaatcc gcctatgggt aaaacccgaa atctattcat tacaatatta
     6961 cgtgatcaag aatggttcgt aaggctcgtc tgtaactaag gaggttttta tgcggcggtt
     7021 tcaatccaag cgcgccttcg gcctcattct cttttctctt tggctggttg tgtcggcggt
     7081 gttttagtgt cagcacggct ttttcacaga cgccatttta ccagggaaag acga
//
LOCUS       DASSWZ010000025         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_113946, whole genome shotgun sequence.
ACCESSION   DASSWZ010000025 DASSWZ010000000
VERSION     DASSWZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113946"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..828
                     /locus_tag="VFN58_00585"
     CDS             88..828
                     /locus_tag="VFN58_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263013193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:286..288,aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET8916511.1"
                     /translation="MNDPLEIERYNYSEFVGSDFSAFRTHLPVGSAAPDFQSTLLETG
                     RPVRLSDYWKKGDLLIEFGSLTUPYCALAAPALEPLAREYAGKDVSFVFLYTREAHPG
                     ENFPAHRSIEQKLSHATIFKEQFNIQRPILVDDLVGTGHKLYGVLPNMTYLIGRGGKV
                     LFRADWTDPPTIEMTLQYVMTSRGRKRQGLHLAPFYAEIVGYRWSDIAKAHEGLRRAG
                     PKAVEDYERVQQRWRKRGPRPGRIEIDD"
     gene            complement(840..1250)
                     /locus_tag="VFN58_00590"
     CDS             complement(840..1250)
                     /locus_tag="VFN58_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET8916512.1"
                     /translation="MTPELERAVKRYTQWFGSYKKSGELVEIQVWLTVNNGSIEFLTL
                     DDSYKVKRIRRNPRVVCYIGDKEGPSVSGTAEIFMGSEAIWRVYRAYWKTHPLIMAFI
                     AFSIRRRIRTHKQVLIRVRPNVPNPLAGVTDPIV"
     gene            1427..1864
                     /locus_tag="VFN58_00595"
     CDS             1427..1864
                     /locus_tag="VFN58_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET8916513.1"
                     /translation="MGEQFSILLARVSLGMFFAISGGNKLFVTSRTRQMYETLACAGI
                     PFPHFMTYFVSSVEFISGCLLIIGLLSSLCCAALIIQMIVAITTVQLATISKGLSFLN
                     WLDDFLYLPETMYIIILIWLICSGPGRVSVDDRIARALGYQDG"
BASE COUNT          503 a          556 c          566 g          486 t
ORIGIN      
        1 gggtagtacc agcaggggga tgaaccacgc gagacggatc gcaacgattc acttcggcga
       61 gtttcgaaag aaatgaaagg acaaccgatg aacgatccac tggaaatcga aagatacaat
      121 tacagcgaat tcgtcggttc cgatttctcg gccttccgga cgcatcttcc ggtaggctcc
      181 gctgcgcctg actttcagtc gaccttgctg gaaactgggc ggcccgttcg attgagtgat
      241 tattggaaaa aaggcgacct gttgatcgaa tttggttcgc tgacatgacc ctactgcgcg
      301 ctggcggcgc cggcgctgga gccactggcg cgggaatacg ccgggaaaga cgtctcattt
      361 gtttttctct acactcgcga ggctcatccg ggcgagaatt ttcctgcgca ccgaagcatt
      421 gagcaaaagc ttagccatgc gacgatcttc aaagagcagt tcaatatcca gcgcccgatc
      481 ttagttgacg acttagtcgg cacaggtcac aagctatacg gagtgctgcc gaacatgacc
      541 tatctcattg gccgcggagg aaaggtgctc tttcgcgccg actggacgga cccgcctaca
      601 atcgagatga cactccagta tgtcatgact tcccgagggc gaaagcgaca gggattgcat
      661 ttggcaccat tctacgcgga gatcgtcggc tatcgctgga gcgatatcgc caaagcgcac
      721 gaagggttaa ggcgtgctgg gccaaaggcg gtggaggact atgagcgcgt tcagcaacgg
      781 tggcgcaagc gagggcctcg gcccggacgc atagagatcg acgattaaaa tcacgcgcct
      841 caaacaatag gatctgtcac gccggccaag ggatttggca cattggggcg gacgcgaatg
      901 agaacctgct tgtgagttct gatccgcctt ctaattgaga acgcaataaa cgccatgatc
      961 aacggatgcg tcttccaata agcgcggtag acccgccaga tggcctcact tcccataaaa
     1021 atttcagcgg tgcctgagac agacggccct tctttatcac cgatataaca aaccacgcgc
     1081 ggattcctac gtatccgctt aaccttgtag gaatcatcga gggtcagaaa ctcgatggag
     1141 ccgttgttga cggtgagcca aacctggatc tctacgagct cacccgattt cttgtaggag
     1201 ccgaaccatt gtgtatagcg cttgacggcg cgttcaagct ctggtgtcat cgggtaggtc
     1261 cagttcccaa ttgggagtcc gaggcaataa gacgaaccat aaaacccacg ctatcacaga
     1321 cgggagtaat caccgatcga ttacattgtc ataggatagc ttgcggagaa actaacgcaa
     1381 tgaagcctta tgcattgatc cggtcagtgc tgctcggaaa ccgttcgtgg gagagcaatt
     1441 ttcaatcttg ctcgcccgcg tttcactcgg aatgtttttt gccatttcgg gagggaacaa
     1501 gctattcgtc actagtcgaa cccgccagat gtatgaaacc cttgcctgcg ctggaatacc
     1561 ctttccgcat ttcatgactt atttcgtctc ctcggtcgag ttcatcagtg gctgcctgct
     1621 catcatcggg ttgctttcaa gcctgtgctg cgctgctcta atcatccaga tgatcgtcgc
     1681 gatcacgacg gtccagcttg cgacgatttc gaaagggctc tcgtttctca actggctcga
     1741 cgatttcctc tatctgccgg aaaccatgta catcatcatc ttgatctggt tgatctgctc
     1801 cggaccggga agggtcagcg tcgatgaccg gattgcccgc gcgctcgggt atcaggacgg
     1861 ataaactcgg gagcgcgcac tcaaagatcg ccgttgtccg tgaaaccggg ggttagctca
     1921 atttgacccc agcttctctt gtacttttcc tccccttgac acggtgacac gccgtgatta
     1981 cgttccctac atctaaacaa aagctttaaa aaggagggct aattatggaa ctagtaagat
     2041 gggatcaggt tgagggtgta aacagatttc agtcccggat caacgagcta ttcgaagaca
     2101 cgttcggtcg g
//
LOCUS       DASSWZ010000026         5202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_43201, whole genome shotgun sequence.
ACCESSION   DASSWZ010000026 DASSWZ010000000
VERSION     DASSWZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43201"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..887
                     /locus_tag="VFN58_00600"
     CDS             366..887
                     /locus_tag="VFN58_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916514.1"
                     /translation="MSRILVVEPRNILRQAIGLALFPDHEVQMAAWLSDSHATVAQEF
                     DLVIVDSAALRATNTLNSQLVRAVQSWKVPTIWIDDTGARQAPARDKLVVLTGPIQKE
                     SLQSAVTKCLGISSSKQNSTTSIPAKEPMATKEPRTATASQGTGPQIIELVDVVAEPP
                     ESRKTKKQPTKTK"
     gene            892..3645
                     /locus_tag="VFN58_00605"
     CDS             892..3645
                     /locus_tag="VFN58_00605"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET8916515.1"
                     /translation="MGLAKAYDHRNVESKWYAHWMQSGAFKPAAGKAPYFSMVIPPPN
                     VTGSLHMGHALNNTLQDILCRYKRMDGYRVLWVPGTDHAGIATQNVVERRLSAQGLTR
                     AQVGREEFIERVWEWKKEAGSAILHQLKALGVSCDWEHERFTMDEGLSFAVREAFVRL
                     WEEGLLYRAQRLINWCPRCKTALADIEVVHEESDGSLWHIRYPLADDPSEALIVATTR
                     PETMLGDTAVAVHPKDKRYQRFAGKKIRLPITDRVVPLLFDSYVDQTFGTGALKITPG
                     HDFNDFEIGERFGLDRISIFDADAKIDGSAFTSRGEKGDWIHRYNLKDRFEARRLIIA
                     ELKEKGFLEKVEMYRHAAGRCYRCQTTVEPYLTPQWFVDIKPLAEPAMQVVREGKIRI
                     IPEGWSNSYFAWMENIKDWCISRQIWWGHQIPAWYCVACDSGNVIRGNNGEHILTKDA
                     RPIVARQAPSGCPNCGGKELLQDPDVLDTWFSSGLWPFSTLGWPHETDDLKSFYPTST
                     LVTGFDILFFWVARMIMMGLKFMDDVPFRDVYIHALVRDEEGQKMSKSKGNVIDPLDV
                     MQEYGTDAFRFTLTAMAAMGRDIKLAQDRIAGYQNFVNKLWNAARFVVMNLGEGSNGQ
                     EVHRRFLASGDLQFADLWIRSRLASTIAEARKAIDAYRFNDYANVLYQFTWHEFCDWY
                     IEMSKLSLNGTIGSDPRQSQRLLVEILEQILLLLHPIMPFVTEEIWHVLEGPLTGPRK
                     SVMLESYPRLETVWIDEQSENKMEFLMNTIRAIRNLRAEVNCPPSREVKVIFHEPNHD
                     LSILRGQEPYLRALARVGSVEYLTSGERPKGAATAVVASVEIYLPLGDMINLQDERTR
                     LTKETSKVEEELARIQKKLSNPQFLSKAKEEVIQKEREKSTQHEEKLRALNLSLERLR
                     EIE"
     gene            3663..4517
                     /gene="nadC"
                     /locus_tag="VFN58_00610"
     CDS             3663..4517
                     /gene="nadC"
                     /locus_tag="VFN58_00610"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET8916516.1"
                     /translation="MDILVSPQIERLIRDSLDEDIGAGDLATMATISAESQGKGLFRA
                     KKDGVVAGLVLLDRIFYFIDPKVEVRLLTKDGSQVKNGKVVAEAEGPVRALLLAERTA
                     LNFLQRLSGTATLTRKYVDAVKDLPCKVLDTRKTTPGLRTLEKYAVRMGGGTNHRIGL
                     YDAALVKDNHIEATGSIAEAVKAVRRHAPFMAKVEVEAGNFKQVQEAIDAGADVIMLD
                     NMTIAQMAEAVRLVNKRAWVEASGGITLDTIRQVAETGVDFISSGALTHSAPVVDFNM
                     KITMNARS"
     gene            4546..>5202
                     /locus_tag="VFN58_00615"
     CDS             4546..>5202
                     /locus_tag="VFN58_00615"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET8916517.1"
                     /translation="MEGPLQLQQVQDGLATRRLGTKLYYFAEIDSTNACARRLAEQGA
                     NEGGVVIAEAQTRGRGRLGRSWVSPRLVNLYVSVILRPQLAPVHAPQLTLMAAVALAD
                     TIASFIGAPPTIKWPNDILVGGKKLAGILTESSCDSERIEFVILGIGVNLNYPVALMP
                     KVIRERATSILALTKNNIKREAFLQRLIQDLDRCYGELEEVGFDSLALRWEALFGLRG
                     K"
BASE COUNT         1250 a         1295 c         1493 g         1164 t
ORIGIN      
        1 tgtgtggatt gggaaaaagc gcctaaaact cgccgtcgat ttgtgatatt gtgggagaaa
       61 accgggcgct tggtttgcag aacatcggcc ataaaaatta gtcgcaatct tgtgtcgcag
      121 ctgtgccaat ccgtgccaca gacacaaata ctggattcac ttgaaccgaa atttggaaag
      181 gcgatttact aggtgagact gaacggaacc ggactagact caaccgaagg gcagtttcct
      241 gaatcctgcg cgtctaccaa ttccgccact ttcgcatctg gatcaatgct cgttgtcctt
      301 cgtatcttat gtaacgtggg gtcatagggt tgtcaagata aagaataccg aggtttgcgc
      361 aacgaatgag taggatcctg gttgttgaac cgcggaatat actgcggcag gcgattggtc
      421 tggcgctttt cccggatcat gaggtccaaa tggctgcctg gttgtcggac agccatgcga
      481 ccgtggcgca ggaattcgac ctagtcatcg ttgattcggc ggcgttgcgt gcgacgaata
      541 ctttgaacag tcagctcgtc cgtgcggtgc aatcgtggaa agtcccgacg atatggatcg
      601 acgataccgg agcgcgccaa gcgccggccc gtgacaagct agttgtactt actggaccga
      661 ttcagaagga aagcctgcaa tcagctgtaa caaagtgtct cggcatttcc tcctcaaagc
      721 aaaatagtac cacgagcata ccagcaaagg agccaatggc gacaaaggaa ccaaggacgg
      781 ctacggcgtc tcaggggacg ggcccgcaaa tcattgagct tgttgatgtc gtagcggagc
      841 cgccggagtc gaggaagacc aaaaaacaac cgaccaagac gaagtaatca tatggggcta
      901 gcgaaagcct acgatcacag aaatgttgaa tccaaatggt atgcccattg gatgcagtcc
      961 ggagcattta agcctgccgc agggaaggct ccgtattttt ccatggtcat tccgccaccg
     1021 aatgtgaccg gatctttgca tatgggccat gcgctcaata acacgctcca ggatatcctc
     1081 tgccgttaca agcgaatgga cggctaccga gtcctctggg ttccggggac ggatcatgcc
     1141 ggtatcgcca ctcagaacgt cgtcgagcgc cgtttgtctg cccaagggct gacccgagca
     1201 caagtgggac gggaagagtt catcgaaaga gtctgggaat ggaagaaaga agcagggagc
     1261 gcgatcctgc atcagctcaa ggcgctgggc gtctcctgcg attgggagca tgagcgtttt
     1321 accatggacg aagggctttc ttttgcggtg cgtgaagctt tcgttcgcct ttgggaagag
     1381 ggtctgcttt accgcgcgca gcgactcatc aactggtgcc cgcgctgcaa gacggcgtta
     1441 gccgacattg aagtcgttca tgaagaatcg gacggcagtc tttggcacat ccgttacccg
     1501 ttggccgacg atccatccga ggcgctgatc gttgcaacga cgcggccgga aacgatgctt
     1561 ggggacaccg ctgttgcggt gcatccgaaa gacaaacgct accagcgttt cgccggcaag
     1621 aagattcgct tgccgattac ggatcgtgtg gtaccgctgc tctttgattc ctatgtcgat
     1681 cagacattcg gaaccggggc gctgaagatt accccgggcc acgactttaa tgattttgag
     1741 atcggtgaac gctttggcct ggatcgaatt agtatctttg acgccgatgc gaaaatcgat
     1801 ggctctgcat ttacttcccg cggcgaaaaa ggcgattgga tccaccgcta caacttgaaa
     1861 gatcgattcg aggcgcgaag gctgatcatc gcggagctga aagaaaaagg ttttctggaa
     1921 aaagtcgaaa tgtaccgcca tgccgcgggc cgttgttacc gctgtcagac cacggtcgag
     1981 ccttatttga cgccgcaatg gttcgtggac atcaaaccgt tagcggaacc ggcgatgcaa
     2041 gtggtgcgag aaggcaagat tcggattatc ccggaaggtt ggagtaactc ctattttgct
     2101 tggatggaaa atatcaagga ttggtgtatt tcgcggcaaa tttggtgggg gcatcagata
     2161 cctgcttggt actgcgtcgc gtgcgattca ggaaacgtca tccgtgggaa caatggtgaa
     2221 catattctta ccaaggacgc gagacctatc gtggcccgac aggcaccaag cggttgtccc
     2281 aattgcggcg gcaaagaatt gcttcaagat cccgacgttt tggatacctg gttttcttca
     2341 ggcctatggc cgttctcaac ccttggctgg ccgcatgaga cagacgatct caagagtttc
     2401 tacccgacct ctaccttggt cacgggtttc gatattttgt tcttctgggt ggcgcggatg
     2461 atcatgatgg gactcaagtt catggatgat gtcccgtttc gggacgttta cattcacgca
     2521 ttggtacgcg atgaagaagg gcagaagatg tccaaatcga aagggaacgt tatcgatcct
     2581 ctcgatgtca tgcaagaata tggcaccgat gctttccgtt tcaccttgac agccatggcc
     2641 gccatgggtc gagatatcaa actggcccag gatcgcatcg ctggttacca gaatttcgtc
     2701 aataagctct ggaatgcggc gcgatttgta gtgatgaacc tgggcgaggg ttcgaacggg
     2761 caagaggtcc atcgacgttt tcttgcgagc ggcgacctgc agttcgcgga cctttggatt
     2821 cgttcgcgtc ttgcgtcgac catcgctgaa gcacgaaaag cgatcgacgc ctaccggttc
     2881 aacgactacg ccaacgttct gtatcaattc acctggcatg agttctgtga ctggtacatc
     2941 gaaatgagca agctgtccct gaacggtact atcggttcag atcccaggca aagtcagcgg
     3001 ttgttggttg agatcttaga gcaaatcttg ttgttgcttc atccgatcat gcccttcgtc
     3061 actgaagaaa tctggcacgt gctagaagga cccctcaccg gcccgcgcaa gagcgtgatg
     3121 ttggagtcct atccacgcct tgagacagtt tggatcgatg aacaaagcga gaataagatg
     3181 gagtttctaa tgaacactat tcgtgccatt agaaatctgc gcgctgaagt gaactgtccg
     3241 ccgagtaggg aggtcaaggt tatcttccat gagccgaatc acgatttatc gattctgcgc
     3301 gggcaggagc cctacctccg ggccctagcc cgcgtcggat cggttgagta tctgacttcc
     3361 ggagagcggc cgaagggcgc ggcgacggcg gttgtggcct ctgtcgagat ctatctccct
     3421 cttggtgata tgatcaactt gcaagacgaa cgcacgcggc tgaccaagga aaccagcaaa
     3481 gtggaggaag agctagcgcg gattcaaaag aaactctcca accctcaatt tctatctaag
     3541 gccaaggaag aagtgattca gaaagagcgg gaaaaatcca cgcagcacga agaaaagctt
     3601 cgtgccctca acctgagcct ggaacggctg cgggaaattg agtaagaaag gaactgtgac
     3661 ccatggatat cctcgtcagt cctcaaattg agcgattgat tcgcgactcg ttggatgaag
     3721 acatcggcgc tggcgatcta gcgaccatgg cgaccatttc agccgagtct cagggcaagg
     3781 ggctgttccg agccaagaag gatggcgttg tggcgggatt ggtgctcttg gatcgaatct
     3841 tctattttat cgatccaaaa gtggaggtac gccttctaac caaggatggc agccaagtga
     3901 agaacggcaa ggttgtggcg gaagcggaag gtcccgttcg cgcgctgttg cttgccgaac
     3961 ggacagctct aaacttcctc cagcgtttgt caggaacggc aaccttaaca cggaaatatg
     4021 tcgacgctgt gaaagacctt ccctgcaagg tcttggatac gagaaaaacg acaccgggac
     4081 ttcggacgtt ggagaaatac gccgtgcgca tggggggagg aacgaaccat cgcatcggct
     4141 tatacgatgc cgctttagta aaggacaatc atatcgaggc cacagggtcg attgccgaag
     4201 ccgtcaaagc cgtaagacgt cacgctccat tcatggccaa agtcgaagtc gaagctggaa
     4261 atttcaagca ggtccaggaa gcgatcgatg ccggggccga tgtgatcatg ctcgacaaca
     4321 tgacgatcgc gcagatggcg gaggcggttc gattggtgaa caaacgcgct tgggtcgagg
     4381 cctccggcgg catcacatta gatacgattc gccaggtggc cgagacgggc gtcgatttca
     4441 tctcgtcggg cgcgctgacc cactcggccc ccgtcgttga tttcaacatg aagattacaa
     4501 tgaatgcgcg ttcttgagac ggataataga caagggtcgg cgcggatgga agggccgctc
     4561 caactccagc aagtccagga cggcttggca accagacgtc tcggaacgaa actctactac
     4621 tttgcggaaa tcgactccac caacgcttgc gcccgacggc ttgccgaaca aggagcgaac
     4681 gagggcgggg ttgtcattgc ggaagcccaa acacgcggtc gcggccgttt gggtagaagc
     4741 tgggtatctc cacgtttggt caacctgtat gtttccgtca tactccggcc gcagctggcg
     4801 ccggttcatg ccccgcagct taccttgatg gctgctgtcg ccctggctga tacgattgcc
     4861 tcatttatcg gtgcccctcc caccattaag tggcccaacg atattttggt tggcggtaaa
     4921 aagctagcgg gaatcttgac cgaatcgtcg tgtgattcag agcggatcga attcgtgatt
     4981 cttgggatcg gcgtcaacct gaattatccg gtcgcattga tgccgaaggt gattcgcgaa
     5041 cgtgcgacgt cgattctagc tctaaccaaa aacaacataa aacgtgaggc gtttttacag
     5101 agattgattc aggacctcga caggtgctat ggagaactcg aagaggtggg tttcgattcc
     5161 cttgcgctgc gctgggaagc cctttttggg ctgcgtggca aa
//
LOCUS       DASSWZ010000027         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1090368, whole genome shotgun sequence.
ACCESSION   DASSWZ010000027 DASSWZ010000000
VERSION     DASSWZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090368"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFN58_00620"
     CDS             <1..444
                     /locus_tag="VFN58_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET8916518.1"
                     /translation="VNRPEFVRKAAETFGSQCIVVAIDAKRVSGHWEVFTHGGRNPTG
                     LDACEWAQKMEQYGAGEILLTSMDRDGTKTGYDLELTREVSDQVRIPVIASGGVGNLE
                     HIYDGLTQGKASAALAASIFHYQEYSIAKCKNYLHSRGILVRLAG"
     gene            complement(552..1763)
                     /locus_tag="VFN58_00625"
     CDS             complement(552..1763)
                     /locus_tag="VFN58_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HET8916519.1"
                     /translation="MPIGTPFHSRTSALCTSQNWRIWSGYLVASSYDLLHDYEYHAIR
                     NAAALIDVSPLFKYDVCGKDALRLVNRVITRDAMKCAVGQAIYTCLCDAEGKVIQDGT
                     VFRLAPDDFRFHLADPGLRWLKLNAVGMEVSIQEVSEQVAALALQGPSSLQILQQVVD
                     VKLSQLRFFRFTSANIGGIPMIISRTGYTGDLGYEVWVAAEHAERVWDVLIEAGRGFG
                     IRPAGMLALDVARLEAGFILLEVDYIGVEKALISSQKCTPFEIGLGWTVDLKKDHFVG
                     LRELRKERERGPSRQIVGLEIDSRDYDQLYQQARLPPQIPLTAWRGGVPVYKETRQVG
                     HATTGGWSPMVKKYIVLATVQKEYVEPGTRVDFEVTVEYRRKTVGATVVKLPFFDPPR
                     KRAVFVSPSEA"
     gene            1873..>2562
                     /locus_tag="VFN58_00630"
     CDS             1873..>2562
                     /locus_tag="VFN58_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8916520.1"
                     /translation="MTKYDAIIIGGGHNGLTSAGYLAKAGKKVLVLERRHLLGGAAVT
                     EEIYPGFKYSVCSYVVSLLRPEVIHDLELPKYDLEIIPLDSTFTPMPDGNYLARWGDH
                     AKTRREIARHSATDAEAYEQYGQLMVQMAMAVKPLLSMIPPKPTSWNPKELFGLASLG
                     NHFRQLGEKLLYDMTKLMTMSSADFLDEWFETEVLKATMSASGIIGTFMGPRSPGTAY
                     VLLHHYMGELDG"
BASE COUNT          626 a          731 c          640 g          565 t
ORIGIN      
        1 gtcaatcgcc cggaatttgt ccggaaagcc gccgaaacat ttggcagtca gtgcatcgtc
       61 gtagctatcg atgccaagcg agtcagcggc cactgggagg ttttcacgca cggcggaaga
      121 aatcctacag gacttgacgc ctgtgaatgg gctcagaaaa tggagcagta cggtgccggc
      181 gaaatcttac tcacaagcat ggatcgggac gggacgaaga ccggctacga cttggaattg
      241 acccgcgagg tctcagacca ggttcgtatt ccggtgatcg cctccggcgg cgtcggtaac
      301 ttggagcata tctacgatgg actaacccaa gggaaggcca gcgccgcgct tgccgcatct
      361 atttttcact accaggaata ttcgatcgcg aaatgtaaga actatctaca tagccgtgga
      421 attctcgtgc ggctcgcagg ctgatgaaaa aaacacatat cctccgtcgc gctcaatcgc
      481 ctcgcctctg aatcttcttg agcagcctgg aaacagagtt tttccaacaa ccgtttagtc
      541 gaggacgcta gctaggcttc gctgggcgaa acgaacaccg cacgtttacg cggcggatca
      601 aaaaacggga gtttgaccac ggttgcaccg acggtctttc gtcgatattc gacagtgact
      661 tcaaaatcga cccgggttcc gggttccacg tattcctttt ggactgtggc tagcacaatg
      721 tactttttga ccatcggcga ccaaccgccg gttgtcgcat gccccacttg ccgtgtctct
      781 ttgtacacag gcaccccgcc gcgccaagct gtcaagggaa tctgcggcgg caatcgagct
      841 tgctgataga gctgatcata atcacgcgag tcaatttcca ggcctacgat ctgcctcgac
      901 ggaccgcgct cacgctcttt gcgtaactcc ctcaagccga cgaaatggtc ttttttcaaa
      961 tcgacagtcc agcctagccc gatctcaaac ggcgtacatt tctgggacga gataagcgct
     1021 ttctccacac cgatataatc gacctccaac aaaatgaacc ctgcctcaag acgtgccacg
     1081 tcgagggcca gcatccccgc cggcctgata ccaaagcccc gtccggcctc tatgagcaca
     1141 tcccagactc gctctgcatg ctccgcagca acccaaactt cgtatccgag atcgccggta
     1201 tagccggtcc gagaaataat catggggata ccgccgatat ttgcagaagt aaatcgaaaa
     1261 aatctcagtt gactcaactt gacgtctaca acttgctgga gaatttggag tgagctaggt
     1321 ccctgaagcg caagcgctgc aacctgttcc gaaacctcct gaatggagac ctccattccg
     1381 acggcattta gcttaagcca gcgcagaccg ggatcggcca aatgaaagcg aaagtcatcc
     1441 ggcgccaacc ggaaaaccgt gccgtcttga ataacctttc cctccgcatc acataaacag
     1501 gtatagatcg cctggccgac cgcgcacttc atagcgtctc gggtgatcac gcgattgacg
     1561 agccttaagg cgtctttgcc gcagacgtcg tatttgaaga gcggcgaaac atcgattaga
     1621 gccgctgcat ttcgaatcgc atggtactca tagtcgtgca gcaggtcgta agagctggcc
     1681 acgaggtatc cggaccagat ccgccaattt tggctcgtgc agagcgccga cgtccgtgag
     1741 tgaaaaggag tcccgatggg catatgctgc tttcgatttt agatttttga ttttacattg
     1801 aaaaaaatcc gtctattgcg ttgacttggt gcacaaaatt accaaaaatg cccgcctaag
     1861 caaatcaaac ccatgacaaa atacgatgct attatcatag gcggtggcca taacggtctc
     1921 accagtgccg gttatttagc aaaagccgga aagaaagtcc ttgtccttga gcggcgccat
     1981 ttgcttggcg gcgccgcagt caccgaagaa atctaccctg gtttcaagta ctcggtttgc
     2041 tcttacgtcg tgagcctctt gcggccggaa gttatccacg atctcgaact tcccaaatac
     2101 gatctcgaaa tcattcccct cgacagcacc tttacaccca tgccggatgg taattatctc
     2161 gcccgttggg gagaccatgc caagacgcgc cgggagattg cgcgccactc ggcgactgac
     2221 gcggaggcct atgagcaata tggccaactg atggtccaaa tggcaatggc agtcaaaccc
     2281 ctgctcagca tgattcctcc aaagccgacc tcctggaatc cgaaagaact ctttggactt
     2341 gcttcgctgg gcaatcattt tcgccaactc ggcgagaaac tcctctatga catgacaaaa
     2401 ctcatgacca tgagttcggc ggattttttg gacgaatggt ttgaaaccga ggttctcaaa
     2461 gctaccatgt ccgctagcgg catcatcggc accttcatgg ggccgcggtc gcccggcacc
     2521 gcctatgtcc tcctgcatca ctacatggga gagctggacg gc
//
LOCUS       DASSWZ010000028         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1203716, whole genome shotgun sequence.
ACCESSION   DASSWZ010000028 DASSWZ010000000
VERSION     DASSWZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203716"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /gene="hslV"
                     /locus_tag="VFN58_00635"
     CDS             complement(<1..386)
                     /gene="hslV"
                     /locus_tag="VFN58_00635"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET8916521.1"
                     /translation="MFSGTTILAIRHGGKVVVVGDGQVSLGQTVMKRSARKVRKLHHD
                     RVIAGFAGATADAFTLFEKFEGKLEQFNGNLKRAAVELAKDWRTDRVLRQLEALLIIA
                     DVNDLLVISGSGDVIEPDDDLIAIGSG"
     gene            complement(565..1473)
                     /gene="xerC"
                     /locus_tag="VFN58_00640"
     CDS             complement(565..1473)
                     /gene="xerC"
                     /locus_tag="VFN58_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET8916522.1"
                     /translation="MENLIQKYVDHLRNERNVSPHTLRNYLSDLVQFSQFLVERKLCL
                     EEGGTVDLRTVDIHVVRTYLAALTKNRKKSSMGRKLAALKGFFRYLVATRQVEKDPLR
                     LIHSPKQEKPLPNFLSVDDVFQLLGAIKVNSSLDFRDCAVLEVFYSTGIRASELVGLN
                     WSDVDFQLGIIRVVGKGSKERIVPIGEVALQSLRDYGQEVRKKWSLPCTGANPVFLNH
                     RGGRITTRSVARVVEKHLREAGIAVKMGPHGLRHSFATHLLNSGADLRVIQELLGHAS
                     LSTTQRYTHLNLDELTAVYDKAHPRA"
     gene            1807..>2364
                     /locus_tag="VFN58_00645"
     CDS             1807..>2364
                     /locus_tag="VFN58_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8916523.1"
                     /translation="MSQRKTTFGLTLSNRAVVTRGTQPKDLIDMAVRAEASGRVYAVW
                     VGDSILSKPRLECMPLLGAIAGHTSRLRLGVACMATIAQRNPVLLALQWSSLDVLSGG
                     RTWLAACMGYPASQHPTAAKELEVMGIASKERPDRLEEMIQALRVLWSDEHASFRGRY
                     YSFDDVNLLPKPVQQPCPIYIAGTPR"
BASE COUNT          541 a          687 c          604 g          532 t
ORIGIN      
        1 cccgacccga tggcgatgag gtcatcgtcc ggttcgatta catctccgct accggaaata
       61 accagcaagt cgttgacatc agcgataatc aagagcgcct cgagctgtcg caaaacccga
      121 tcggtccgcc aatccttggc cagctccacc gccgctcgtt tcagattgcc attgaattgc
      181 tccagcttgc cctcgaattt ctcgaacaag gtgaaagcat ctgcagtcgc cccagcaaag
      241 ccggcgatca cacggtcatg atggagcttc cggaccttgc gcgcgctacg tttcatcacg
      301 gtctggccca gactgacctg accgtccccc acaaccacca ctttcccgcc gtggcgaatc
      361 gctagtatgg ttgtaccgct gaacattttg gattttagat tgccgatttt ggattcatta
      421 tagccctaaa tgtttttgca ggctgctcaa aaagactcag aggcgaggcg cgcgaagatc
      481 gatgagcgga ggcgtacttt tttgtacgct gacgtgaaat cggtcgagcg caacgaagca
      541 tatgagtctt ttttcagcag cctgctaggc ccgcggatgg gccttgtcat agactgccgt
      601 cagttcatcc aaattcaggt gcgtgtagcg ctgggtcgtc gaaaggctgg catgaccgag
      661 caattcttga atgacccgca aatcggcgcc gctgtttaac agatgggtag caaagctatg
      721 acgcagcccg tggggaccca ttttgacagc gattcctgcc tcccgtaggt gtttctcgac
      781 cacgcgcgcc acgctcctgg tcgtaatcct tccgccccga tggttcaaaa agaccggatt
      841 tgcccctgtg cagggcagac tccatttctt gcgcacttct tgaccgtagt cgcgaagcga
      901 ctgcagcgcc acctcgccga tcggcacaat ccgttccttg gagcctttcc caacaacgcg
      961 gataataccc agttgaaaat caacatcact ccaattgagg cccaccagct cgcttgcgcg
     1021 aattcccgtg gaatagaaaa cctccaagac cgcgcagtct cgaaaatcca aagagctatt
     1081 cactttgatc gcgcccaaca actgaaagac atcgtccacc gaaagaaaat tgggcagcgg
     1141 tttctcctgc ttgggactat gaatcagccg tagcggatcc ttttcgacct gacgcgtggc
     1201 aaccagataa cgaaagaatc ctttcagcgc ggcaagtttg cggcccatcg aactcttttt
     1261 tcggttcttg gttaaagcgg ctaaatatgt cctaacgacg tgaatatcga ccgtgcgtaa
     1321 gtccacggtc ccgccctcct ccaggcagag ctttctttca accaggaact gggaaaactg
     1381 gaccagatcg ctgagataat ttcgcaaggt gtgcggcgac acgttgcgct cgttacgcag
     1441 atgatcgacg tatttttgaa tcaagttttc cacgattcca ctcggcactg taagaataac
     1501 acgcgtgtga aaaaaacgaa atcatccctt cgatctcgcc tgggactatt tatcgaccga
     1561 ggaaaaggca acatacactg tagaccgaga agtgagcatc ggagcgctcg ggcacaggag
     1621 cgtaaagctg caacgactcc tagggagctt ggttatgagc ttgcatttgt tccttattct
     1681 tcactccacc actctaattt gcaggcctgc tcaagagaca ggcccattga ccgcgaccgg
     1741 agcagctcgc tcctcggtct gtttcttctt gacaccagag accagcctcg gcttatattc
     1801 gccgtcatgt cacagagaaa aacaactttt ggcctgacgt tatccaatcg ggctgtggtt
     1861 accagaggca cgcagccgaa agatcttatc gatatggccg taagagcgga ggcgtcggga
     1921 agagtttacg ctgtttgggt aggcgacagc attctcagca aaccgcggtt ggagtgcatg
     1981 ccgctcttag gtgccatcgc cggacatacc tcgcggctcc gtctcggagt cgcctgcatg
     2041 gcaaccatcg cgcaacgcaa tcctgtgctg cttgccttgc agtggtcgag tcttgatgtc
     2101 ctatccggcg gccgcacgtg gctggccgcc tgcatggggt acccggcgag ccaacatcca
     2161 accgccgcca aggaactcga ggtcatgggc atcgcgagca aagagcgacc ggacaggctg
     2221 gaagagatga ttcaggccct gcgcgtcctc tggtcggatg aacacgccag tttccgcggg
     2281 aggtattatt cctttgacga cgtgaacctt cttcccaaac ccgttcaaca accatgcccc
     2341 atctatatcg cgggtacccc tcgg
//
LOCUS       DASSWZ010000029         6018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_901, whole genome shotgun sequence.
ACCESSION   DASSWZ010000029 DASSWZ010000000
VERSION     DASSWZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFN58_00650"
     CDS             complement(<1..967)
                     /locus_tag="VFN58_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET8916524.1"
                     /translation="MGRSQSARAKRKEQAMNREKIVFLDAATYGDISLQSCIDQWDCT
                     IHQVTSPPETSQRLIGQSVAVTNKVVIDRAVFSSAAAKELKLIAVAATGTDIIDREAA
                     KEFDVRVCNVPGYATQSVAQFTLALILELATRAARYGAAVRSDAWQKSPVFTLLDFPS
                     IELKGRKLGIIGYGDIGRAVAKMARGFGLEVLIAARPGSTEPISSGRVPLRELFQQAD
                     VITLHCPLTPQTKSLINAETLSLMKPSALLVNTARGALIDETALIRSLREKRLAAAAL
                     DVISREPPPADHPIIRAAKELDNLLVTPHTAWSAREARERLLGEVE"
     gene            complement(919..1731)
                     /gene="mazG"
                     /locus_tag="VFN58_00655"
     CDS             complement(919..1731)
                     /gene="mazG"
                     /locus_tag="VFN58_00655"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET8916525.1"
                     /translation="MKNERAGETFAKLLDVMAQLRGDNGCPWDKEQSHASLRPFLLEE
                     SHELLDALDKGDSAQIREELGDLLHQIIFHCQIAAEGGAFTAEDVVRDLKDKMVRRHP
                     HVFSGKTLPDSDTVLKQWAKIKAKENDGETSRSALGNLPKSMPGLARAQTITERASQV
                     GFDWPDIVPVWGKVEEELAELKTACSSGEKQRTGEELGDLLLSLVNLSRFLGLQSEEV
                     LAQATERFIKRFSYIETRLHDAGKSPTLSSLEIMDRLWDEAKALERSEKNKR"
     gene            1892..2740
                     /locus_tag="VFN58_00660"
     CDS             1892..2740
                     /locus_tag="VFN58_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8916526.1"
                     /translation="MATAVIDGIEVYYETKGSGSPLLMFAPGGFDATIDKWVSASAWK
                     DINALDVLATEHTVVVYDRREAGRSGGRVERLGWGSYTRQAKDLLDHLRIDSAYVMGG
                     CMGCSVALAFGVHYPERARALLLHWPVGGYRWKVNGLERFRRHYDFTKANGVKAVVER
                     AQTGKSFWSDPESGPWASVIARNRQFAENFADQDLERYLGVILTAGRGFFDRDTATGA
                     EPEEVMGIKAPALIIPGDDPSHATSGAHYLRECLPKNEFWNVMPPEQTTQKVCDRILE
                     FCRAHS"
     gene            2831..3550
                     /locus_tag="VFN58_00665"
     CDS             2831..3550
                     /locus_tag="VFN58_00665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8916527.1"
                     /translation="MDVTGSMLQLNTLDGKMEAYEARPKDKGSYPGIVVLMEAFGLND
                     HIKKVTERIAQEGYVAIAPDLYHRESERVVPYSDLQKAIGIMNRLQDPKVMDDVGAAI
                     AHLKSQSIVKAGSIGVAGFCMGGRFTYLSAAHHNKDVQAAVVFYGGGIPMGKPSPLSR
                     TEEIASPMYLFFGGKDPLIPMDHVDQIKAKLQDEKKSFTMEVYPEATHGFFCDERPSY
                     HEASAKDAWEKTKAFFAQHLK"
     gene            3635..4531
                     /locus_tag="VFN58_00670"
     CDS             3635..4531
                     /locus_tag="VFN58_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HET8916528.1"
                     /translation="MPKTRKRELVTLGLIQMSTHESPDANLATALSRIRAAAEKGAQI
                     VCLQELFRSRYFCQNEDHQNFRLAETIPGPSTEALGKLARDNKIVIVASLFEKRSAGI
                     YHNTAVIIDADGTIAGKYRKMHIPDDPLYYEKFYFTPGDLGFPSFQTRYAKIGALVCW
                     DQWFPEAARLASLGGAQILFYPTAIGWIPKESRAVAQCQRNAWEIIQRSHAVANGVYV
                     AAVNRVGREGKLRFWGRSFVAGPFGEILACASGERDEIVIAKCDLSKIEETRQSWPFL
                     RDRRIDAYGYLQSRFIESFDKH"
     gene            4562..5638
                     /locus_tag="VFN58_00675"
     CDS             4562..5638
                     /locus_tag="VFN58_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET8916529.1"
                     /translation="MTKTNSSQATPQSLGYSMPAEWMPHRATWLAWPHNRQTWPTQLE
                     TVREVWVRIIQALALNEQVLLLVNDEQTGQDVVLRLKNIRTVMKNISILTIPTVDVWM
                     RDYGPTFLTRAVNENPLVLNDWIFNGWGKKYKSYEDDDRVAKEMASLLQVPVFNHSVV
                     LEGGSIEVNGAGTCLTTEQCLLNKNRNPHMSRGEIEQFLKEALGVKHLIWLGEGIIGD
                     DTDGHIDDIARFINPTTVVCVLESNPKDENYAPLRENYERLQGARDQDGNRLSIVTLP
                     CPAPVYCAGSRLPASYANFYIANEGVLTPVFDDPNDYKALGILQELFPERRVVGLPCK
                     ALVAGLGAIHCVTQQEPSILACRP"
     gene            5676..5888
                     /locus_tag="VFN58_00680"
     CDS             5676..5888
                     /locus_tag="VFN58_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916530.1"
                     /translation="MGQIIQLEAIHQARRRRSEKVSMEQCVQLLEWNLKKSLDDYFSS
                     PREERSMRATQIRKLSEILEYALRLL"
BASE COUNT         1339 a         1563 c         1695 g         1421 t
ORIGIN      
        1 cttcgacttc tccaagcaat cgctcgcgtg cttcgcgcgc gctccaggcc gtgtgcgggg
       61 ttacgagcag attgtcgagt tccttcgctg cccgaatgat cgggtgatct gctggcggcg
      121 gctcgcggga gatcacatcc agtgcggcag cggctaagcg cttttcacgc aaagaccgaa
      181 tcaatgccgt ctcgtcgatc aacgctcccc gagccgtgtt gaccagcaag gcggaaggct
      241 tcattaggga aagggtttcg gcgttgatga gacttttggt ctgcggcgtt aacgggcaat
      301 gcaaggtgat cacgtcggct tgctggaata gctccctcaa tggaactcga ccggaagaga
      361 ttggctctgt cgaaccaggg cgggccgcaa tcagtacttc taaaccgaat cctcgtgcca
      421 tctttgcgac cgcccggcca atatcgccat agccgatgat gccgagtttt ctccccttga
      481 gttcgatgct cggaaaatct agcaaggtga atacaggact cttctgccat gcgtcggaac
      541 gcacggccgc gccgtaacga gcggcgcggg tcgccaactc caggatgagc gccagggtga
      601 attgtgccac ggattgggtt gcataacccg gaacattgca aaccctgaca tcgaactctt
      661 tggctgcctc gcgatcgata atatcggtcc cggtggcggc gacggcgatg agtttcagct
      721 cttttgccgc agctgaactg aagaccgcac ggtcgatgac gaccttattg gtcaccgcga
      781 ctgattgccc aatcaatctt tgggaagttt cgggcgggct cgttacctgg tggatcgtac
      841 agtcccattg gtcgatacaa ctctgcagtg aaatatcgcc ataggtggcg gcgtcgagaa
      901 atacgatttt ctcgcggttc atcgcttgtt cttttcgctt cgctcgagcg ctttggcttc
      961 gtcccacaaa cgatccatta tttctagaga cgaaagtgtc gggctttttc ccgcatcgtg
     1021 caatcgggtc tcgatatagc tgaatcgttt gataaaccgc tcagtcgctt gtgccagcac
     1081 ctcttccgat tgcagtccga gaaagcgcga tagattcacg agggagagaa gcaggtcgcc
     1141 aagctcctcg ccggtgcgct gtttttctcc ggatgaacag gccgttttaa gttccgccag
     1201 ctcttcttcg accttgcccc ataccgggac tatatccggc caatcgaaac cgacttgtga
     1261 ggcccgttcg gtgatcgtct gagcccgtgc aagcccgggc attgacttgg gaagatttcc
     1321 caacgcagac ctagacgtct cgccatcgtt ctctttggcc ttaattttcg cccactgttt
     1381 caagacggtg tcagagtccg gtagtgtctt gccggaaaag acgtgtggat ggcgtcggac
     1441 catcttgtct ttcaaatccc ggacgacgtc ttccgcggtg aaagcaccgc cttccgcggc
     1501 aatttggcag tgaaaaatga tctggtgcaa gagatcgccg agttcctcgc ggatttgcgc
     1561 agagtctccc ttatcgagcg cgtccaggag ttcatggctt tcttccaaga ggaacggtct
     1621 tagcgacgcg tggctctgct ctttgtccca tggacatccg ttatctccgc ggagttgggc
     1681 catgacatct aacagcttgg caaacgtttc cccagcccgt tcattcttca ctaacctacc
     1741 ctccgcaatg ggtttcgggt taagcttccc tctgaatcga cagcgtgtta gatcttacgt
     1801 gtcgctcgcg ctgatgtcca gtaatactct ggcaagatac tctaattgca agaacagctg
     1861 gatttgagaa tcggacaaag gagggacgac gatggcaaca gccgttattg acgggattga
     1921 ggtctattat gaaacaaaag gttcgggttc acctctgttg atgtttgcgc cgggtggctt
     1981 tgatgctacg atcgacaaat gggtttcagc gagcgcgtgg aaagacatta atgcgctcga
     2041 tgtgctcgcg acagagcata cggtcgtcgt ctacgatcgg cgcgaagcgg gcagatcggg
     2101 tggacgggtg gagcgcttgg ggtggggaag ttacacgagg caggcaaagg atctcttgga
     2161 tcatttgcga atcgattccg cctatgtcat gggcggctgt atggggtgtt cggtagcgct
     2221 ggcatttggt gttcattacc cggaaagagc tcgcgctctg ctgctccatt ggccggtggg
     2281 aggatatcgt tggaaggtaa acggcttgga gcggttccgg cgtcactacg atttcaccaa
     2341 ggcaaacgga gtgaaagcgg ttgtcgagcg cgcgcagacc ggaaagagtt tctggtccga
     2401 tccggagtcg ggcccctggg cgtcggtgat cgcacgcaat cgacaattcg ctgagaattt
     2461 tgccgaccaa gatctggagc ggtatctcgg agtcatatta accgctggac gcggcttctt
     2521 cgatcgggac accgccactg gagccgagcc cgaagaggtg atgggcataa aagcaccggc
     2581 gttaatcata ccgggtgacg atccgtccca cgctacctct ggagcccatt atctgcgcga
     2641 gtgtctgcct aaaaacgagt tttggaatgt gatgccgccg gaacaaacaa cgcagaaggt
     2701 ctgtgatcgc attctggagt tttgccgggc ccatagctga tttttcatgc atgggctcca
     2761 cacggtggac cggcaaaaaa gaatctgata ggaaaaactt cccacccaag aaattcttaa
     2821 ggaggcgttt atggacgtca cgggttccat gctgcaactc aatactttgg acggaaagat
     2881 ggaagcctac gaggcgcgtc cgaaggacaa agggtcttat ccgggaattg tggttttgat
     2941 ggaagctttt ggcctcaacg accatatcaa aaaagtaaca gaacgaattg cccaggaagg
     3001 ttacgtcgcc atcgcgccgg atctctatca tcgggagtcg gaacgggtcg ttccctacag
     3061 cgatttgcag aaagctatcg gcattatgaa ccgcctacaa gatcccaagg taatggatga
     3121 tgtgggtgcg gcgattgcgc atttgaagtc acaaagcatt gtgaaagcgg ggtccatcgg
     3181 cgtggccggc ttctgcatgg gtgggcgctt tacttatctt tcggcggctc atcacaacaa
     3241 ggatgtccaa gcagcggtgg tgttctacgg cggcggtatt cctatgggta agccgagccc
     3301 tttgtcgcgg acggaagaaa tcgcctctcc gatgtatctt ttctttggcg ggaaggatcc
     3361 gctcattccg atggatcacg tggatcagat caaagccaag ctccaagatg aaaagaagag
     3421 cttcacgatg gaagtctatc ctgaagcaac ccacggattt ttctgtgacg agcgaccaag
     3481 ctatcatgaa gcatctgcga aagacgcctg ggaaaagacc aaagcgtttt tcgcccaaca
     3541 tttgaagtaa gcgatcgtgc aagtcttgca ggagagcaag gcacaaaacg cctttcctct
     3601 cttttgttcg aatatagtcg ggaaagttgc gattgtgcca aaaactcgga agcgagagtt
     3661 agtgactctg ggtctcattc aaatgagcac ccatgagagc ccggacgcga acttggcaac
     3721 agcgttgtca cggatccgtg ctgcggccga gaagggcgct cagattgtct gcctccagga
     3781 attgttccgt tcgcggtact tttgccagaa cgaagatcat cagaacttta gacttgccga
     3841 aaccattccc ggcccgagta cggaggctct tggaaagctc gccagggaca acaagatcgt
     3901 cattgtggcg tccctttttg aaaagcgatc cgccgggatc tatcacaata cggccgtcat
     3961 catcgacgcc gatggaacca tcgcgggcaa gtatcgcaag atgcatatcc cggacgaccc
     4021 gctttattac gaaaagttct attttactcc aggcgatctg ggtttcccga gttttcaaac
     4081 acgatacgct aagattggtg cgcttgtctg ctgggaccaa tggtttcccg aggcggctcg
     4141 cctcgcttca cttggcggag cgcagattct tttttacccg acggccatcg gctggattcc
     4201 caaggagtcc cgtgcggtcg cccagtgcca gcgcaatgcc tgggaaatta tccagcgctc
     4261 ccatgcggta gccaacggtg tctatgtcgc tgcggttaat cgagtggggc gggagggaaa
     4321 actgagattc tggggtcgct cgtttgttgc cggtccgttc ggagaaatcc tggcctgtgc
     4381 gagcggtgaa cgggacgaga ttgtcattgc gaaatgcgat ctgagtaaga ttgaagaaac
     4441 acggcaaagt tggcccttcc tgcgtgatcg acgcatcgat gcttatggct atttgcaatc
     4501 acgcttcatt gagtcattcg acaaacactg attgcggttc ttcctctgcg gtccttgcac
     4561 catgaccaag acaaactcga gccaagctac cccgcaatca cttggctact ctatgcccgc
     4621 ggagtggatg ccccatcggg cgacctggct cgcctggccg cacaatcgcc aaacctggcc
     4681 aactcaattg gaaacggtgc gggaagtttg ggtccggata atccaggcgc tggccctaaa
     4741 cgaacaggtt cttctattgg ttaatgacga acagacgggg caggatgtgg tcttacgact
     4801 caagaatatt agaacagtaa tgaagaatat ttcgatcctg acgattccta cggtggacgt
     4861 ctggatgagg gactacggac cgacatttct cacgcgagcg gttaacgaaa atccgctggt
     4921 gctgaatgat tggatcttca acggttgggg caaaaaatac aaatcctacg aggatgacga
     4981 tagggtcgcc aaagaaatgg cgtccttgct gcaggttccg gtgttcaatc attcggtcgt
     5041 gctcgaagga ggctccatcg aggttaacgg cgcggggacc tgtctcacga cggaacagtg
     5101 cttgttgaac aaaaatcgca atccccatat gagccgcggg gagattgagc agttcctcaa
     5161 agaggcactg ggagtgaagc acctcatctg gttaggagag ggcattatag gggatgacac
     5221 cgatggacat attgatgata tcgcccgctt tatcaatcca acgacggtgg tttgtgtttt
     5281 ggagtcaaat ccgaaggacg aaaactacgc tcctctccga gaaaactacg agcggcttca
     5341 aggggcgcgg gatcaagacg gcaaccgcct gtccatcgtg actctccctt gccccgctcc
     5401 tgtttactgt gcggggtccc gtttgccggc gagctacgcc aacttttaca tcgccaacga
     5461 aggggtcctg actccggtat tcgatgatcc gaatgactac aaggctctgg gcatcctgca
     5521 agagcttttt cccgaacgtc gggtggtggg cctgccttgc aaggcgctag ttgccggctt
     5581 gggtgcaatt cactgtgtga cgcagcaaga gccgagcatt ttggcttgca ggccttgaca
     5641 attcatcgcg ctcgttctta tcctacggta ttcgcatggg tcagatcatt cagcttgagg
     5701 cgatccatca agcgcgacgc cgacgctccg agaaggtcag catggagcaa tgtgtgcagt
     5761 tgctcgaatg gaacctgaag aaaagcctcg acgactactt ctcttcgcct cgcgaagagc
     5821 gatccatgcg cgcgactcag attcgcaagc tcagcgaaat actcgagtac gcactgagac
     5881 ttctgtaacc cagcccaatc agtcgtggaa tttctaagtc cgattgcact tattttgctt
     5941 ttttccgttg cccttttgct tgtggggtgg ttcttcctca gtcgttttga acccttttgc
     6001 gagatccttc gcgctccc
//
LOCUS       DASSWZ010000030         4188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1345253, whole genome shotgun sequence.
ACCESSION   DASSWZ010000030 DASSWZ010000000
VERSION     DASSWZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345253"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFN58_00685"
     CDS             complement(<1..656)
                     /locus_tag="VFN58_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HET8916531.1"
                     /translation="MLGIVVSLSRELKSLTRQTIPVGSWKAITDNTLVALSGIGADRA
                     YMAGSVLVSQGATALLSWGCAAALDDRVSPGCLILPERIIGANGEIHRVSTEWHRRLY
                     LALESKHSVRTEALVESDVIVKTSAEKRSLARRTQAAATDMESAAQARLAKDYGLPFI
                     AIRAIVDTPSTEIPENVLKALDPEGNINLWKLLSSTMPADWIKIVRLGIQFNAAQRTL
                     "
     gene            complement(656..2617)
                     /gene="shc"
                     /locus_tag="VFN58_00690"
     CDS             complement(656..2617)
                     /gene="shc"
                     /locus_tag="VFN58_00690"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET8916532.1"
                     /translation="MGHGTILSTDHQAIQKSKIKTPDGLHLSQLERAIQHAASALLGL
                     QHPDGYWCFELEADCTIPAEYILMMHYMAEVDDQLERKLATYLRARQGEDGGWPLYPR
                     GPAEISCSVKAYYALKLAGDDPKLPHMAKSRQTILRLGGASRANVFTRIMLAIFEQVP
                     WRAVPFLPVECMLLPRWFPFHLLKVSYWSRTVMVPLFVLYTSRARAQNPKQVSIQELF
                     INDPWLETDYFPVHSALNRALLILDRLGLKLYRWVPKRLRRRAIKKAEAWMLERLNGE
                     GGLGAIFPAMINAYEALGLLGYPVNHPLRATQRRAIDDLLVVKEQSAYCQPCVSPVWD
                     TAWGCIALQQVANERSHKAVKKAFHWLAEKQLLQEPQDWEFNRPKVRSGGWPFQFKNS
                     HYPDLDDTAAVAYAMHKSGDKSFGKTVQRAAEWICGMQSRNGGFASFDADNDHTYLNE
                     IPFADHGALLDPPTEDVSGRCVILLTQLATSPQCQSVKKKCLDYLFRSQEPDGPWYGR
                     WGTNYIYGTWSVLMGLEEAGISREHQAVRRAAAWLENVQRADGGWGEGCDTYFDPSKR
                     GRGERSTSFHTAWALLGLMSSGEVRSDSVGRGIDFLLRTQQSNGLWSDPDFNAPGFPR
                     VFYLKYHGYDKYFPLLSLARYRNLTNEET"
     gene            complement(2640..3797)
                     /locus_tag="VFN58_00695"
     CDS             complement(2640..3797)
                     /locus_tag="VFN58_00695"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET8916533.1"
                     /translation="MLPFRDESAMSKLTSPPGSDRIDRARRKLWLSDDDFQSEMLEGV
                     SRTFALTIPQLPTELCRVVSNAYLLCRIVDTIEDEPVLSGTQKNSFCQQFLKTLNVAR
                     NAEPFSRHLCAALSSQTPPAEHELIRNVPRVLRITRGFSEPQREALQQCVRAMAKGMG
                     QFQLRGEKQGLQSLGDLDQYCYYVAGVVGQMLTRLFCLHSPEVAKHHDALMALAVSFG
                     QGLQMTNILKDVWEDYQHGACWLPRKIFAEEGFDLSDLTTARNRQGFERGVQRLIGIA
                     HRHLRNALAYTLLIPKQETGIRDFCLWAIGLAVLTLRKINNHLDYTDGSQVKISRLSV
                     KGTILATQLARQNDTLLKLLFYLAAWPLPLVAHLTGLQSQSTNAGKANQAR"
     gene            complement(3946..>4188)
                     /locus_tag="VFN58_00700"
     CDS             complement(3946..>4188)
                     /locus_tag="VFN58_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET8916534.1"
                     /translation="YAQSLGLAFQITDDILDAHEVSSDNSNHKGIANYVSVAGAAKAS
                     ERVKGLLTACLREMQPYGSAAEPLREIARYVAQRTE"
BASE COUNT          919 a         1127 c         1145 g          997 t
ORIGIN      
        1 ttcagggttc tttgcgcagc gttaaactga ataccgagtc gcacaatctt gatccaatcc
       61 gctggcatgg tgctactgag aagtttccag agattgatat tgccttcagg atcgagcgcc
      121 tttaggacgt tttctggaat ctccgttgat ggagtgtcga caatggcccg gatcgctata
      181 aagggaagtc cgtagtcttt ggcaagccgc gcttgtgctg cactctccat atccgtcgct
      241 gccgcttgcg tccgtctggc gagcgagcgc ttttccgctg aggttttcac aatgacatca
      301 ctttcaacga gagcctctgt gcgaaccgag tgtttggatt ccaatgctag atagagacgg
      361 cgatgccatt ccgtgctgac ccgatgaatt tcaccgtttg ccccaataat acgctcggga
      421 aggatcaggc atcccgggct gacgcggtcg tccagtgctg ccgcacaacc ccagcttagc
      481 agggctgttg ctccctgcga aaccaaaacc gaccccgcca tgtatgcgcg atctgctccg
      541 atcccggaca gggcgaccaa tgtgttatct gtgattgctt tccacgagcc caccgggatc
      601 gtttgacggg ttaggctctt tagctctcga gaaagcgaaa caacgattcc gagcattatg
      661 tttcctcgtt ggtcaagtta cgatagcgcg ccaacgacaa caggggaaag tatttgtcat
      721 agccgtgata ctttaaataa aaaacccgcg gaaaaccggg tgcattgaaa tcgggatcgc
      781 tccaaagtcc gttggactgc tgtgtgcgca ggagaaaatc gatgccgcgg ccaaccgagt
      841 ccgagcgaac ctcgccgctg ctcatcaagc cgagcaaagc ccatgccgtg tggaaagagg
      901 tgctgcgctc tccacggccc cgtttggacg gatcaaaata agtgtcgcat ccttcacccc
      961 atccaccatc ggcgcgctgg acattctcaa gccatgcggc ggcgcgtcgc accgcttggt
     1021 gttctcgact tatgcctgcc tcttccaacc ccataaggac agaccaggtg ccgtagatgt
     1081 agtttgtacc ccaccttccg taccatgggc cgtccggctc ctgagaacga aaaaggtagt
     1141 cgaggcattt tttcttaacg gattggcatt ggggtgaagt cgccagttgc gtgagcaata
     1201 tcacacaccg tccgctcaca tcctctgtgg gcggatcgag cagggcgccg tgatcggcga
     1261 aggggatttc gttgagatag gtgtgatcgt tatcggcatc aaatgaggca aagcctccgt
     1321 tcctggactg catgccgcaa atccattcgg ctgcgcgttg caccgtcttg ccaaaactct
     1381 tgtcgccgct cttatgcatc gcgtaagcga ccgctgccgt gtcgtcgaga tcgggatagt
     1441 gagaattttt gaactgaaag ggccaaccgc cgctcctgac tttaggacga ttaaactccc
     1501 agtcttgagg ctcttgtaat aactgttttt cagccaacca atggaaggct tttttgaccg
     1561 ctttgtggct ccgctcgtta gcgacctgct ggagagcgat acagccccat gcggtatccc
     1621 atacgggcga cacacagggc tggcaatagg cgctttgctc tttcaccacc aggagatcgt
     1681 cgatcgctcg tcgttgcgtc gcgcgtagcg gatggttaac aggatagcca agtaaaccta
     1741 gcgcttcata ggcattgatc atggccggaa atatggcacc taagccgcct tcgccattga
     1801 gccgctccag catccaggct tccgcctttt ttatggcgcg gcggcgcagc ctttttggaa
     1861 cccaccgata gagctttaat cccaggcggt ccagtataag gagggcgcga ttgagagctg
     1921 aatgaactgg aaaatagtcc gtttccagcc aagggtcatt gataaagagt tcctggatcg
     1981 agacctgttt gggattttgg gcacgtgccc tagacgtgta gaggacgaat agcgggacca
     2041 ttacggttcg tgaccaataa gagaccttta agagatgaaa gggaaaccag cggggcagca
     2101 gcatacattc caccggcaaa aacggtacgg ctcgccaagg aacctgctca aaaattgcga
     2161 gcatgattcg agtaaacaca ttggcacgag aggctccgcc caggcgaagg atggtctgcc
     2221 gggatttggc catatggggt aatttaggat cgtcaccggc aagcttcagc gcatagtagg
     2281 ctttgacaga gcaactgatc tcagcgggac cacgggggta gagaggccaa ccgccgtcct
     2341 ccccttggcg ggcgcgtagg taggtggcaa gttttctttc cagctggtcg tcgacctcgg
     2401 ccatataatg catcatcaga atatactcgg ccgggatggt gcaatcggcc tccagctcga
     2461 aacaccagta accgtccggg tgttgcaagc cgagcagggc gctggcggca tgttgaatgg
     2521 cgcgctccag ttgagacagg tgcagaccgt ctggagtctt gatcttgctt ttttggattg
     2581 cctggtgatc cgtgctgagt atggtgccgt gccccatgaa gtcaggccat ccagtgaact
     2641 cagcgcgcct gattcgcttt accggcgttt gtactttgtg attgcaagcc tgtcaggtga
     2701 gccactaatg gtaatggcca tgcggcgaga tagaaaagaa gcttcaggag agtatcgttt
     2761 tgtcgcgcca gctgtgtcgc taagatggtt cccttaacgc tcagccttga tatcttgacc
     2821 tgacttccgt ccgtgtagtc gaggtggttg ttgatcttgc gcaacgtcaa caccgccaac
     2881 ccgattgccc agagacagaa gtcacggata ccggtttcct gtttcgggat gagtaaagta
     2941 taagctaagg cgtttctcaa gtgtcgatgc gcgatgccga ttaggcgctg cacgccacgc
     3001 tcgaacccct gtcggtttcg cgctgtcgtt aagtcgctga gatcgaagcc ttcttcagcg
     3061 aaaatctttc ggggcaacca acacgcgcca tgctgatagt cttcccacac gtctttaagg
     3121 atgttggtca tctgcaagcc ctggccgaaa gataccgcca aggccattaa ggcatcgtga
     3181 tgcttggcga cttccggtga gtgaagacaa aacaggcggg tcagcatttg gccgacaacg
     3241 cccgcgacat aatagcagta ttgatcgagg tctcccaagc tttgcaatcc ctgtttttcg
     3301 ccgcgtagct ggaattgtcc catgcccttg gccatggcgc ggacacactg ctgtagcgct
     3361 tcgcgctgtg gctcggagaa accgcgggtg atgcgcagca cacggggaac gttgcggatc
     3421 agctcgtgct cggccggagg tgtttggctc gagagtgctg cgcacaggtg ccgggaaaac
     3481 ggctcggcgt ttcgggcaac gtttaaggtc ttcaagaact gttggcaaaa ggagtttttt
     3541 tgcgtgccac ttaaaaccgg ctcgtcttcg atggtgtcaa caatgcggca aaggagatag
     3601 gcattggaga cgactcggca aagctctgtc ggaagctgtg gaatggtcaa ggcaaatgtg
     3661 cgggaaactc cctctaacat ttccgactga aagtcgtcat cgctgagcca caattttcgc
     3721 cgcgcacgat cgatacgatc tgagccagga ggcgacgtca gtttagacat ggcgctttcg
     3781 tccctaaaag gcagcatgtt ttatcctccc tttcactatg gctacggctg gcaacctcat
     3841 cctattcttc cccatacgtc gatctggagc atatgtccag cgctttatcc tgatttttct
     3901 tccaatctcc ttaccttagc cgaccggctt tacccgctcc actgattact ctgttctttg
     3961 agccacgtac cgggcaattt ctcgcaaagg ttccgccgca cttccgtagg gttgcatttc
     4021 tctcaagcat gcggtcaaaa ggcctttaac acgttcactc gcttttgcgg ctcccgctac
     4081 ggatacgtaa tttgcgatcc ctttatgatt cgagttgtcg ctggaaactt catgggcatc
     4141 caaaatgtca tccgtgatct ggaaagctaa acccaaagat tgggcgta
//
LOCUS       DASSWZ010000031         7493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_637657, whole genome shotgun sequence.
ACCESSION   DASSWZ010000031 DASSWZ010000000
VERSION     DASSWZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637657"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFN58_00705"
     CDS             <1..282
                     /locus_tag="VFN58_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET8916535.1"
                     /translation="LTKNNIKREAFLQRLIQDLDRCYGELEEVGFDSLALRWEALFGL
                     RGKRVRVEMTDHIMIGMAKGIDRDGALIVEDDRGERQRIIAGDVFPLED"
     gene            293..1087
                     /locus_tag="VFN58_00710"
     CDS             293..1087
                     /locus_tag="VFN58_00710"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET8916536.1"
                     /translation="MLLAIDIGNTNIVLGLYQGKKLVTHWRLRTEAERTADEYGVIIS
                     QLISSKGFRCNQIQSIVASCVVPPMVSVTQELATKFFELEPLMVGPGIKTGMPILYDS
                     PKDVGADRIVNGIAAYEKYRDACIVVDFGTATTVDFISKRGEYVGGAIAPGLLISLEA
                     LFQRASKLPRIEIVKPKEVVGKNTVNSIQAGIFFGYVGLVDGIVQRIQKENEVQAKVV
                     ATGGLAPLVASECSTISEVDEFLTLEGLRIIHERNSLQEVKARAHK"
     gene            1110..3209
                     /gene="fusA"
                     /locus_tag="VFN58_00715"
     CDS             1110..3209
                     /gene="fusA"
                     /locus_tag="VFN58_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET8916537.1"
                     /translation="MATAELEKLRNVGLLGQGGSGKTSLGEAMLFAAGVTQRLGKVQD
                     GTSVLDHEPEEIKHYVSISTAFHTLAWKKHPFQLIDTPGYAAFLADSINCMRAFGGAV
                     FVLNPSVGIRVESERLWDKANDNRIGRVFFASKMDHEQDNVVERIDSMLEGLEVKGVH
                     LQMPIGVEANFKGVVDLLSMKAYLFEGDSGRFTEGEVPGDLKGAAEEMRQKLLESVSE
                     TDDELLEKYLDGKELSTDELKKAIREQTRQGKLYPVLYGSALKQIGIPQLLDAIIDYL
                     PSPLDEGEMEGTNPITGEAEKRARQPTAPFSAYVFKTIIDPFAGKLSVMRVASGKIAS
                     DGVCYVPNKQVKEKIGHMFRLEGKKQDIVKEASAGDIVAAAKLKDVSTGDTLCDEKSP
                     IQYEGPVHFSPVISFALEPKSKADEEKVPQGLHRIMEEDPTIEVHRDEQTRDFILSGM
                     GQQHVEIIVEKLKRKYSAEVVLKAPKVPYKETIRASASAQGRLKKQTGGRGQYGDTWI
                     KIEPLPHGKGFEFVDEVVGGAIPRNYIPAVEKGIKEAMAGGVIAGYPMVDVRVRLYDG
                     SYHDVDSSDMAFKIAGSLGFKSAVEKAKPVILEPIMNMEITVPDESMGDVIGDLNSRR
                     GKVVGMDTKGHSQVIKSKVPMSEVLKYAPDLRALTSGRGEFQMEFSHYEELPPHLAEK
                     VIKEAKARQAADGEHKA"
     gene            3226..3435
                     /locus_tag="VFN58_00720"
     CDS             3226..3435
                     /locus_tag="VFN58_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916538.1"
                     /translation="MEQSFDQFNAALLYCNQCRRAMPVRERLLLVLPDGELYDYTCQG
                     CNSSVGSKTERVGLKPSVAKPRLGG"
     gene            3441..4469
                     /locus_tag="VFN58_00725"
     CDS             3441..4469
                     /locus_tag="VFN58_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916539.1"
                     /translation="MTVIDSDAHVLETERTWDYMLQSERAYKPRIVATPNDPTSGGES
                     WLIDDVYIGKARNVGHETPKEAREMDDINARLKHMDELDVDVQVLYPTIFLRPFTRRP
                     ELELAVTRSYNRWLVDIWKRAPERLRWVAVLPLLTMDKALEEARFAKENGACGIFMRG
                     LEGDKRLSDSHFFPLYEEAGRLDLPVCVHSATGSFAVHDFFLHECGFSKFKLAVVGSF
                     HALIFHEIPDKFPKTKFAFIEVSSQWVPYAIHDFARRFERGGKKVDKQDVLRKNRIYV
                     ACQTDDDLPYVLKYSGENQLVIGTDYGHNDTSSEILALRKLKEDGAVPPEIVNKILDD
                     NARALYGL"
     gene            4502..5527
                     /locus_tag="VFN58_00730"
     CDS             4502..5527
                     /locus_tag="VFN58_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916540.1"
                     /translation="MVIDFQAHIYPEAYLDEMKRLDGAVVLEAPDPHSGMTYFYDKKL
                     GCRINTATFQGQNIAHRLEHMDQLGVDIHVLTIPAPGADRFEGEGAISIARIANNAVA
                     AICRQHPNRFIGFFTLPTCSIKDSLDELERSVNELGLRGFGCFANLNGKPLDREELLP
                     IYERLARYKLPVYIHPTAPLSTESTGIDIMPTLIFGWAFDSTVAMTRLVYGRVLERFP
                     EISFVVADVGGVLAFFAQRAINIYSGRTEEIRQRYGLQENPLDSFRRFYVDTADHPAS
                     TLKCVKEFFGVDRLVLGTNYPYGPEEGCVLVRNSLKAIDGLELNERDKGKILGGNAQR
                     ILGLGGL"
     gene            5524..7125
                     /gene="ggt"
                     /locus_tag="VFN58_00735"
     CDS             5524..7125
                     /gene="ggt"
                     /locus_tag="VFN58_00735"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8916541.1"
                     /translation="MNFNKSARPVIMGRNGMVSAGHSLGALAGVRVLQEGGNAIDAAL
                     AAGFVMAVVKPESCGPGGDLFALVFMKKNGRVEAINSSGPAPAKASIEYFREKGLKSV
                     PTSGPLSVAVPGAVDGWLELHRKYGTKDLSRLTSDAIDLARGGFPLHLELTEAIEELS
                     SEFPWVDRCFRRPMGEPRPGKLVVQKGLGDVLEKISRNGRDGFYSGEVAETICRTLKA
                     EGGLLTEDDLQSNIARWLDPLSTSYRDYLVFEQPPVSQGFMVLEMLNIIEGWPLHTGE
                     IQGADSIHYQVGAKKLAFEDRIRHLEDPDFGDPKIPMLISKEHAAKRRELLADAIHRP
                     QTAATALGGDTTYLCATDRDGNAVSLIQSIFAAFGSRVIAGDTGVIMNNRLCSFGLDP
                     SKANSLKPGKRPAHTLNTYMVFKSGEFFAVGGSPGADEQPQTNLQVLHNIIDLGMDPQ
                     AAVEEPRWSHQPGTPPRDQLPEHLRMEEGFDPQVVDRLSRRGHKVSVVDRWSFGSAKV
                     ILKDDNTGTWMAGADPRREAYALGW"
BASE COUNT         1798 a         1837 c         2196 g         1662 t
ORIGIN      
        1 ctaaccaaaa acaacataaa acgtgaggcg tttttacaga gattgattca ggacctcgac
       61 aggtgctatg gagaactcga agaggtgggt ttcgattccc ttgcgctgcg ctgggaagcc
      121 ctttttgggc tgcgtggcaa acgggttcga gtcgaaatga ccgaccacat catgatcggc
      181 atggccaaag gaatcgatcg agacggcgcc cttattgtag aggacgaccg aggagagcgt
      241 cagcggatta ttgctggcga cgtgtttccg ttggaagatt gaaatcaagc atatgcttct
      301 ggcgatcgat atcggcaata ccaacattgt tcttggtctc taccagggca aaaagctggt
      361 cacccactgg cgtctccgga ccgaagcgga acgaacggct gacgaatacg gcgtgatcat
      421 ttcacagctc atctcctcaa aggggtttag gtgtaatcag attcaatcta tagtcgcctc
      481 gtgcgtcgtc ccgccgatgg tcagcgtgac ccaagaactg gccaccaagt ttttcgagct
      541 cgaacctctc atggtcggtc ccgggattaa gactgggatg cccattttat acgatagtcc
      601 caaggacgta ggcgccgatc gcattgtcaa cggcattgcg gcctatgaaa aatatcgcga
      661 cgcctgcatc gtggtggact ttggcaccgc gacgaccgtg gattttatat cgaaaagggg
      721 cgaatacgtc ggcggcgcta tcgcgccagg cttgttaatt tctttggaag cattgttcca
      781 acgggcctcg aaactgccac gaatcgagat cgtgaaacct aaagaagtgg tgggaaaaaa
      841 taccgttaac tccatccaag cgggaatctt tttcggctat gtgggtcttg tggacggtat
      901 cgttcagcgt atccagaaag agaacgaagt tcaggccaaa gtcgtggcta cgggagggtt
      961 ggcgcccctg gtggcgtcgg aatgttccac gatcagcgaa gtcgatgaat tcctgacctt
     1021 ggagggactg cgcatcattc acgaaaggaa ttccctacaa gaagtgaagg cgagagcgca
     1081 caaataaaag gaggagggat cctatagtta tggctacagc ggagctggag aaacttcgta
     1141 acgtcggcct tctgggtcaa ggcggctcag gaaaaacctc gttaggggaa gcgatgctgt
     1201 ttgcggcggg ggtgactcaa cggttgggca aggttcaaga cggcacatcg gtattggatc
     1261 atgagccgga agaaatcaaa cactatgtgt cgatctcgac ggcgtttcat acgctggcat
     1321 ggaagaaaca tccattccaa ttgatcgata cgccgggcta cgccgctttc ctggcggatt
     1381 cgatcaattg catgcgcgct ttcggcggtg ctgtatttgt gcttaacccc tccgtcggca
     1441 ttagggtcga gtccgaacga ttgtgggata aggccaatga taaccggatc ggtcgggttt
     1501 tcttcgcaag caagatggat catgagcagg ataatgtagt tgaacgtatc gattcaatgt
     1561 tggaaggtct agaagtcaaa ggggttcacc tccaaatgcc tatcggtgtc gaggcgaact
     1621 tcaaaggagt ggtggatctg ctttctatga aagcgtatct ctttgaagga gatagtggaa
     1681 gatttaccga aggcgaggtt ccgggtgatc tcaagggcgc cgcggaggag atgcgccaaa
     1741 aactgttgga aagcgtgtcg gaaaccgacg acgagcttct ggaaaaatac ctggacggga
     1801 aagagctatc tacggacgag ctgaagaaag ccattcgcga gcaaacacgt caaggaaaac
     1861 tctatccggt tctctacggt tcagcgctca agcagatcgg cattccgcaa ttgctggatg
     1921 ccatcatcga ctatttgccg tccccattgg acgaaggcga aatggaaggc acgaacccga
     1981 taaccggcga ggctgagaag cgggctcgcc aaccaaccgc tccgttttcc gcctacgtgt
     2041 tcaagacgat catcgatccg tttgcgggaa agctctccgt catgcgcgtc gcttcaggca
     2101 agatcgcctc agatggcgtc tgctatgttc ccaacaagca ggtcaaagag aagatcggcc
     2161 acatgttccg tctcgagggt aagaaacagg acatcgtcaa ggaagcttct gccggtgata
     2221 tcgtggcggc ggcgaaactg aaagatgtct ctaccggcga taccctgtgt gacgagaaat
     2281 cgccgatcca gtatgaaggg ccagtgcatt tttcgccggt tatttctttt gctctcgaac
     2341 cgaaaagcaa ggcggacgaa gagaaggtac ctcagggctt acacagaatc atggaagaag
     2401 acccgacgat cgaggtccac cgcgatgagc aaacgcggga ctttatcctt tcgggcatgg
     2461 ggcagcagca tgtcgagatc atcgtcgaaa agctcaagcg caaatatagc gcggaagtcg
     2521 tcctcaaggc ccccaaggtg ccgtacaaag aaaccatccg ggccagcgcg agcgcccagg
     2581 gaagactgaa aaagcaaacc ggcggacggg ggcagtacgg cgatacctgg atcaagatcg
     2641 aaccgcttcc ccatggcaag ggctttgagt ttgttgatga ggttgttggc ggcgctattc
     2701 cgcgaaacta tatccctgca gtggaaaaag ggatcaagga agcgatggcc ggaggcgtta
     2761 tcgccggata tccgatggtg gatgtgcggg taaggcttta tgacggttcc taccacgatg
     2821 tggactcgtc ggatatggcg ttcaaaatcg ccgggtccct gggctttaag agtgccgttg
     2881 agaaggcaaa gccggtgatt ctcgaaccga tcatgaacat ggaaataacc gttcctgacg
     2941 aaagtatggg ggacgtgatc ggtgatttga acagccgtcg cggaaaagtt gtcggtatgg
     3001 ataccaaagg acacagtcaa gttattaaat ccaaagtgcc catgtcggaa gtcctaaaat
     3061 atgctcccga tttgcgcgcg cttaccagtg gccggggaga attccagatg gagttttctc
     3121 actatgagga gttgccgccg catctggcgg aaaaggtgat caaggaggcg aaagcaagac
     3181 aagcggccga tggcgaacac aaggcttgag aattgaaagt ttcttatgga gcagtctttc
     3241 gatcagttca acgcggcgtt gctttactgc aaccagtgcc gcagggcgat gccggtaaga
     3301 gaacgcctgc ttttggtgct cccggacggc gagttgtatg attatacctg ccagggttgc
     3361 aatagctctg tcggttccaa aacggaacgg gtcgggctta agccatcagt cgctaaaccg
     3421 agattgggag gttgattgaa atgacggtca ttgattccga tgcccatgtt ttagaaacgg
     3481 aacgaacctg ggattacatg ttgcagtcgg agcgagcgta caaaccccgg atcgtggcga
     3541 caccgaatga cccgacttca ggtggtgagt catggttgat cgacgatgtg tatattggga
     3601 aggcgagaaa tgtcgggcac gagactccta aagaagccag ggagatggat gatatcaatg
     3661 cccgcctcaa gcacatggac gagctggacg tcgatgtgca agtactctat ccgacgattt
     3721 ttttgcggcc ctttacgcgc cggccggagt tggaactggc ggtaacccgg agctacaacc
     3781 gttggctcgt cgatatctgg aagcgtgccc cggagcggct gcgctgggtg gcggtgctgc
     3841 cgctgctgac gatggataaa gcgctggaag aggcgcgctt cgccaaagag aacggcgcct
     3901 gtggtatctt tatgcgcggc ctcgaaggag acaagcgact cagcgactct cattttttcc
     3961 ctctgtatga agaagccgga cggctggatt tgccggtctg cgtgcactcg gccacgggaa
     4021 gtttcgcggt gcatgatttt ttcctccacg aatgcgggtt ttccaaattc aagctcgctg
     4081 tggtggggtc gtttcacgcc ttgatcttcc acgagatccc ggacaagttt cccaagacca
     4141 agttcgcttt catcgaggtg agctctcagt gggtgcccta tgcgattcat gattttgcca
     4201 ggcggttcga gcgcggcgga aagaaagtgg ataagcaaga cgtgctgcga aaaaaccgaa
     4261 tctatgtcgc gtgccaaacc gatgatgatc ttccctacgt gctaaaatat tcgggcgaaa
     4321 atcagctggt catcggtacc gattatgggc acaacgatac ttcttcggag atcctggcgc
     4381 tgcgcaagct caaagaagat ggcgctgtgc cacccgagat cgttaataaa attcttgacg
     4441 acaacgccag agccctgtac ggcctttaat tcatagtatc tcatcgcgat cctgcatcat
     4501 tatggtcatt gattttcagg ctcatattta tcctgaagcg tacctcgatg agatgaaacg
     4561 cctcgatggc gcggtggttt tggaagcgcc ggatccgcac agcggcatga cctactttta
     4621 cgacaaaaag ctcggatgcc ggatcaacac ggcgaccttc caggggcaaa atattgcaca
     4681 tagattagag cacatggatc agttgggagt cgatatccat gttttgacca ttccagcccc
     4741 cggcgcagac cgttttgaag gcgagggcgc cattagcatt gcaaggattg ccaacaatgc
     4801 tgtggcggca atctgtcggc agcatcctaa tcgctttatc ggatttttca ccttaccgac
     4861 ctgctcaatt aaggattcgc tcgatgagct ggagcgaagt gtaaacgagt tgggactcag
     4921 aggctttggc tgcttcgcga atctcaacgg caaacccctg gaccgtgagg aactgttgcc
     4981 gatctatgaa cggctggcac gatacaagtt acccgtctat atccatccga ccgctccgtt
     5041 atccaccgag tccacaggga ttgatatcat gccgacgttg atctttggct gggcgtttga
     5101 cagcaccgta gcgatgaccc gtttggtcta cggtagagta ttggaacgat ttccggagat
     5161 cagctttgtc gtagccgatg tcggtggagt tttggcattc ttcgcccagc gggcgatcaa
     5221 tatctacagc ggccgcacgg aggaaatccg gcagcgatat ggattgcagg aaaatccgct
     5281 cgattcgttc aggagatttt atgtcgacac cgccgatcac cccgcgtcga cgctaaagtg
     5341 tgtgaaggaa ttcttcggcg tcgacaggct tgtgctcggg accaattatc cctacggtcc
     5401 ggaggaagga tgtgtgctgg tcagaaatag cctgaaagcc atcgatggtt tggagctcaa
     5461 tgagcgggat aaaggaaaaa tcctcggcgg caatgctcag agaattctcg gtttaggagg
     5521 attatgaact tcaacaaatc ggcgcgaccg gtcatcatgg gacgaaacgg aatggtttcc
     5581 gcaggtcaca gtctcggagc gttggctggg gttagagtcc tccaggaggg cggcaacgcc
     5641 atcgatgccg cgcttgcggc gggatttgtg atggcggtgg taaagcccga atcttgcggt
     5701 cctggcgggg atctttttgc gctggtcttt atgaaaaaaa acggcagggt cgaggcgatt
     5761 aattccagcg gtccggcgcc tgccaaggcc agcatcgaat atttccggga aaagggactg
     5821 aaaagtgttc cgacgtcggg tccgttgagc gtggcggtcc ccggggcggt cgacggatgg
     5881 cttgaactgc acagaaagta tggcaccaag gatctgagcc ggcttacatc tgatgcgatt
     5941 gatcttgccc gcggtgggtt cccgctccat ttggaactga cggaggcgat cgaggagttg
     6001 agctctgagt tcccctgggt tgaccgctgt tttcgccggc cgatggggga gcccaggcct
     6061 gggaaacttg tcgttcaaaa ggggctggga gacgtgctgg aaaaaatctc tcgcaacggc
     6121 cgcgatggat tttacagtgg cgaagttgcg gagactatct gccgaacact taaagccgag
     6181 ggtggcttac taacggaaga cgatttgcag agcaatattg cgcggtggct cgaccccctc
     6241 agcacgagct atagggatta tctggtcttt gaacagcccc cggtgtctca gggattcatg
     6301 gttctggaga tgttgaatat catcgagggg tggccgctgc atacgggaga gatccagggc
     6361 gctgacagca ttcactatca ggtgggagct aagaagctcg cttttgaaga tcgcatccgc
     6421 cacctcgaag accccgactt cggcgatcca aaaatcccaa tgctgatttc gaaggaacat
     6481 gcggcaaagc gacgagagtt acttgccgac gcgattcacc gaccccagac cgctgcgacc
     6541 gcgttgggag gggacaccac ctatctctgt gccacagatc gcgacggcaa tgcggtttcg
     6601 ttgatccaaa gtatcttcgc ggcttttggt tctcgtgtga tcgccgggga tacgggcgtt
     6661 atcatgaata atcggctttg cagcttcggg ctcgacccga gcaaagcgaa ctcgctaaaa
     6721 ccgggcaaac gacccgcgca cacactcaac acctatatgg tcttcaagag cggtgagttc
     6781 tttgctgtgg gcggaagccc aggtgctgac gagcagcccc aaacaaatct gcaagttctc
     6841 cacaatatca tcgacttggg tatggatcct caagctgcag ttgaggagcc gcgctggagt
     6901 caccagccgg gcacgccgcc ccgcgatcaa cttcctgagc acttacggat ggaagagggt
     6961 tttgatccgc aagtagtcga ccgtttgagc aggcggggcc acaaagtttc cgtagtggat
     7021 cgctggtctt ttggtagcgc caaggtaatc ctcaaagacg ataatactgg aacgtggatg
     7081 gcaggcgccg atccccgacg agaagcctat gcactcggct ggtaaactcg cgctaacgat
     7141 gatcgtgact ctgagtccaa ctagaaacgg ccgccagctg tgacccgctg gggattatcc
     7201 tatgcactgg cgctgcgtgc catcagtcct tggaagcgca acaaacaaaa gatttcgatc
     7261 tttccgttat cgagacgggc ttcgttgttc ttgaaagtag accgcggtat ttgaagcggc
     7321 ggcgaactat tgcgagcgag ctattccggt gtcagcggcg tgccgtccaa cgtagcgacc
     7381 ggtgagcgag ccaaccgcca gcagtacaat agtgaattgc gttcggcgca acgctttctc
     7441 tactccgttg gttcgaatag ttcgccgcca cttcaaatac cgcggtttat ttt
//
LOCUS       DASSWZ010000032         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_779227, whole genome shotgun sequence.
ACCESSION   DASSWZ010000032 DASSWZ010000000
VERSION     DASSWZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779227"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1443)
                     /locus_tag="VFN58_00740"
     CDS             complement(349..1443)
                     /locus_tag="VFN58_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916542.1"
                     /translation="MKRNNLRLLLFAIAFSAFMAALTQSFSAERPPFYQGKTLTFIIN
                     FAAGGPTDIESRIFAKHLTKHIPGQPTVVVQAMGGAGGLTAVNYIGEVAKPDGLTAAY
                     FTGSLFQHQIKDPALRVDISKFGFITGVQGVTVSYIRSDVRPGIKKPSDFVKAERFRA
                     AGLGISSSKDVRFRLSFDLLGLKYDYVTGYNSSSTARLAVERNEAQYHDETLPSYRAA
                     VEPQMVQTGIVTPLYYTDLVTPSGEVLVSQDVPELQPFSYYYKEIFGKPPSGIKYEAL
                     KAANMSSTNMTRMVLMPPKVPPETITIMRQAFLSLAKDEDFLTDAVKTMRFQPRFEVG
                     QSGEALFKRASQTSPEIITFLRQYIEQANK"
     gene            1893..>4173
                     /locus_tag="VFN58_00745"
     CDS             1893..>4173
                     /locus_tag="VFN58_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8916543.1"
                     /translation="MRLAKAFARISNGLLANHKPRRFSLRTQLVLLVLAVVLPLLVLA
                     AVMFLRDVQFQRAALERGMKDTARALSLALDREVGKLHAVLETLAASPSLDSRDFKSF
                     NELALRATEQHKGSRIVLFDRSGQQIINTLRPFGASLPNILEEARKSPEATEGGLPLG
                     SPPTVKSVLETGEPVISDLFMGLVSKRPSLATTVPVIRDGNIAYGLSMVTLPANFATL
                     LREQGLPHEWNAVIVDRKGTIISRNPDAERFVGRPVSPKLRGLLSQSDQGWSASQTSD
                     GVQLYSAFARSTLTGWAVAIEAPQAVIDAAVNRSLRIIGGAAAVLLFVALGTALLLGR
                     RISGPISMLARSAEAIQRGERVEIKTSGVKEISELHSAVLAAGEATREWAAERERAVI
                     EKRLRRLAEVSATLAESIDFEKTLTRLVEFMVPDYADWCSIDLLREGSNICRRVAVRT
                     SRKDKEPIAEEFYRNYAPDLSRPHPILTAIKTGRSDYMLDLDQRWTDQHARNERHQFL
                     LEQMELSGVIVVPLRVQGRVAGALSMFASRYSGRKYTVADVEFAEEVGRRASIALDNA
                     WLYGEVQRELTERKQAEEALRESEERFRSTFENAPVGMAHVGLDGRWLRVNDTLCKIT
                     GYSRMELLAKTFGDITHPDDLEKSWARVQSLLAGELSTYSMEKRYLRKDGSLVWVEIT
                     ISLRRDADGRPLYLIGAIEDITDRKRAQEELGESEERFRSYFELGLIGMAITSPSKGI
                     LEVNEEICKTLGYERDELLR"
BASE COUNT          899 a         1092 c         1218 g          964 t
ORIGIN      
        1 tcccttgtcg gggggcggcg agatacgcgg ccagatccgc gtcatcggca aatagtgttt
       61 cgtcgcgcgc cgggcatcgc gaacacgtga gtcaatcggg gtcgatgcat gccagtggaa
      121 cgttgaagaa gcgtctagtg acacgtgtaa cgcttaggtc gtgaatccgg cgacttaaca
      181 gagacagaag acgcgtatcg gcgggttggc aaagcactcg ttcgacgcgt cttctgttag
      241 ttcactgttt tgagacgatc gtccggacgg tgatacgagc gtccgactag ggggacgggt
      301 gtaactcgag tattccagtg ctccgatcac ccggaagtgt gtccaacttt acttgttagc
      361 ctgctcgatg tactgtcgga gaaaagtgat tatttcaggc gatgtctgtg acgctcgttt
      421 gaaaagagcc tcacctgact gaccgacttc gaagcgcggc tgaaaacgca tggtcttgac
      481 cgcgtccgtt aggaagtcct catccttcgc tagcgacaga aaagcctgcc tcataatggt
      541 gattgtttcg ggcggtactt tcggcggcat caataccatt cgggtcatat tggtactcga
      601 catgttggca gctttgaggg cttcgtattt gattcccgaa ggtggtttgc cgaagatttc
      661 tttgtagtaa tagctaaagg gctgaagttc gggcacgtcc tggctcacta agacttcgcc
      721 gcttggcgtg actaaatccg tgtaataaag cggcgtcacg ataccggttt ggaccatctg
      781 cggttccact gccgcgcggt aactgggcag tgtttcatcg tggtattggg cttcgttgcg
      841 ctccactgcg agccgcgcag tggaacttga gttgtagccg gttacataat cgtacttcag
      901 tccaagcaag tcgaaggaca accgaaagcg tacgtccttg gagctgctga taccgagccc
      961 cgcggctctg aaccgctccg ctttcacgaa gtccgagggc ttttttatcc ctggcctaac
     1021 atcggagcgg atatacgaga ccgtgacacc ctgtacacct gtgatgaatc caaacttgct
     1081 gatatcgacg cgcaaagccg gatccttaat ctggtgttgg aagagacttc cggtaaaata
     1141 agccgcagtg agcccgtcag gttttgcaac ttcaccgatg tagttgaccg ctgtgagacc
     1201 tccggccccg cccatggctt gaaccaccac ggtaggctgc cccggaatgt gcttggttaa
     1261 gtgtttagcg aaaattctgc tttcaatatc ggtggggcct ccggcggcaa agttgattat
     1321 gaacgtcaag gttttccctt gatagaaagg aggccgctcc gccgaaaaac tttgcgtcaa
     1381 tgccgccatg aacgcagaga aggcgatagc gaagagtagc aatcgcaagt tatttcgttt
     1441 cattattctc tcctctcagt agtcggtaag cgctacttag aaaatccgcc ggcatagaca
     1501 cgggtcatac tgcacccaaa ggccgccctg ccgcccttca tactgcaact gctttgactt
     1561 gtctatcacc atcgtgggcc tggttttgtt caagtcgatg atggatcgct tggctcgcta
     1621 tcgcccgtag gagcgcagct cttttttgtg gcaaagtcgt ggcaaagcag aaagattcga
     1681 ttgtgttgtt tttcttgtta gagatgcgac gagatcatca ttcttgtatc gttccttttc
     1741 gcttcttgcc cgatgccagt ttccaccgtg gggcttcgtg cccttgacag cgctgcactg
     1801 cgctggtgta aacttgatcg ttcttgcgct tttccctttg agagataact acgcgccagt
     1861 gcacatccaa aggcagcgcg gggagtatag ggttgcgcct ggctaaagct ttcgcccgga
     1921 tttcaaacgg cctccttgca aatcacaaac cgcgccggtt ttctctgcgt acccagttgg
     1981 tcctgctcgt actcgcagta gtgctgccac ttctggtctt ggctgcggtc atgttcttgc
     2041 gggatgtgca gttccagcgc gccgctcttg agcgaggaat gaaggatacg gcccgtgcgc
     2101 tgtcgcttgc cctcgaccgc gaggtgggaa aactgcacgc tgttttggaa acgcttgcgg
     2161 cgtcgccgtc tctcgattcg agagacttca aatccttcaa cgagcttgct ctacgagcga
     2221 ctgagcaaca caagggttcc aggattgtcc tcttcgaccg ctcgggccag cagatcataa
     2281 acacactccg gccgtttggc gcttctctac ccaatatttt agaagaagca aggaagagcc
     2341 cggaggcaac tgaagggggc ttgccgcttg gaagccctcc gactgtgaaa tcggtgctgg
     2401 agaccggtga gcccgtgata tccgatcttt tcatgggact cgtttccaaa cgaccttcac
     2461 ttgccactac ggttcccgta attcgggacg gtaacatcgc atacggccta agtatggtga
     2521 ccctgccggc aaacttcgcg actcttttga gggagcaagg gcttccacac gagtggaacg
     2581 cggtgattgt ggacaggaaa ggaactatca tctcgcgcaa ccccgatgct gagaggtttg
     2641 tcggccggcc tgtgtcgccg aaacttcgtg gactgctcag tcaatctgat cagggatgga
     2701 gcgccagcca gacttcggac ggagtccaac tctacagcgc gttcgcgcgt tcgacgctta
     2761 caggatgggc cgtagcaata gaggccccgc aggctgtaat tgatgctgcg gtaaaccgct
     2821 cgctgcggat cataggaggg gccgccgcgg tgctcctgtt tgttgcgctc ggcacagcac
     2881 ttcttctcgg caggcgcatt tcaggcccga tttccatgct tgcgagatcc gcggaagcaa
     2941 ttcagcgcgg agagcgggtc gagataaaaa cttccggagt gaaggaaatc tccgagcttc
     3001 attccgctgt gcttgccgca ggggaggcca ctcgggaatg ggccgcggag cgcgaacgcg
     3061 cagttatcga aaaacgactg cgacggctgg ccgaggtaag cgcaaccctc gctgaatcga
     3121 ttgattttga gaagacgctc acgcgcctgg tcgaattcat ggtgccggac tatgcggatt
     3181 ggtgctccat cgacctgctg cgagagggct caaatatttg ccgccgtgtc gccgtgcgga
     3241 caagcagaaa agacaaagaa ccgattgcgg aagagtttta ccggaattat gcgcctgacc
     3301 tgagccgtcc ccatccgatt cttactgcga taaagactgg ccgctctgac tacatgctcg
     3361 atctggatca gagatggacc gaccaacacg ctcgaaatga gcgtcaccag tttctactgg
     3421 aacaaatgga attgagcggc gtgattgtcg tgccgttgcg tgtgcaaggc cgagtggcgg
     3481 gggcactctc catgttcgcc tcgcgatatt ccgggcggaa atacacggtc gccgacgtcg
     3541 agttcgcgga ggaagtcggc cgccgcgcct cgattgccct tgataacgcc tggctttatg
     3601 gagaagtgca gagggaattg acagagcgta aacaggcaga ggaagccctg cgggagagtg
     3661 aagagaggtt tcggagcacg tttgaaaacg caccggttgg catggcacac gttggcctgg
     3721 atggccggtg gttgagggtg aatgacacgc tctgcaagat taccgggtac tcgcgcatgg
     3781 agcttttggc taaaaccttc ggcgacatca cgcatcccga cgatctggag aagagctggg
     3841 cgcgtgtcca aagcctgctc gcaggcgaac tgtcaacata ctcgatggag aagcgctatc
     3901 tccgcaaaga tggttctctg gtctgggtgg agatcactat atctctgcgg cgcgatgcgg
     3961 acgggcggcc gctttatttg atcggtgcga ttgaggatat caccgaccgc aagagggctc
     4021 aggaggagtt aggcgaaagc gaagaacgat tccggagcta cttcgaactc ggcttgatcg
     4081 gcatggcgat cacatcgccg agcaaaggca tactcgaggt caatgaagag atttgcaaaa
     4141 cgctgggcta cgaacgcgac gaactgttgc gca
//
LOCUS       DASSWZ010000033         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_15129, whole genome shotgun sequence.
ACCESSION   DASSWZ010000033 DASSWZ010000000
VERSION     DASSWZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15129"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFN58_00750"
     CDS             <1..451
                     /locus_tag="VFN58_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916544.1"
                     /translation="GKATAFMWQLAPYNLWGNPLNWDKVRGEWFQKQLAVWQRFAPNL
                     DENNILSTDSTTPLDIERHDRNMYCGDWMVGEYSGDQALENRPFPGWGNYRTPIDGLY
                     LCGSSCHPGGNITGAPGYNAARVIAESLGIKPWWKPLDFKEHMKGLR"
     gene            547..1290
                     /locus_tag="VFN58_00755"
     CDS             547..1290
                     /locus_tag="VFN58_00755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET8916545.1"
                     /translation="MRLGGKVAIVTGGARHIGAAYCRRLAVEGAALVVADVLDGGSVV
                     KEITTEGGKAIALKVDVSREEDTARMAAEALQAFGRIDILVNNAAIFLNIQRHPFYEI
                     SGEEWDQVSAVNIKGPFLCAKAVFPQMKQQRSGKIINISSSTVFAGTPLFLHYVTSKA
                     ALVGMTRSLAREVGEFGICVNAIAPGLVQHEGMNAPKEFTEFQLKTRSIKRLQTPEDL
                     LGVLVYLASSDSDFVTGQTILIDGGSVFY"
     gene            1423..1848
                     /locus_tag="VFN58_00760"
     CDS             1423..1848
                     /locus_tag="VFN58_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916546.1"
                     /translation="MLVVAALWWQWAQTQKQSQVGKAAPGVNSLTAAAVSGIWRGEVT
                     YSGGTTYTERFLFQVEGDKLFGTASFLGFKRGIEDGRIDGDKISFNVRFHEAVEGVST
                     ERTNRYAGSVSDNQIHFKMQDDRGERPVEFVVRKENRAE"
     gene            1861..3810
                     /locus_tag="VFN58_00765"
     CDS             1861..3810
                     /locus_tag="VFN58_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HET8916547.1"
                     /translation="MAYHAFVAMPFGIKEQIDFNRVYGELIKPALEDAGFEVFRADEE
                     MRAGDIRTDMFQELLMADLVVADLSIDNPNVWYELGVRHALRARGVIGIMCRRNYMPF
                     DVYTDRVLTYQIKDNPPGAAIPDPERLEDGKKKLAEFATQTMDSWYERKISPVYHLLP
                     ALKEPHFKLLQVEGAKEFWSWAMRIEVARKGNRPGDILVFAEEAPNIGLRVEAYRSAG
                     NGLRSLGQFALALDQYEKALALAPDDLESRRQKGLLLGRLKQSDAAKEWLQNLVKEVP
                     DDAESWALLGRVEKDSWISGWRADGKTVEQMQEDAAADDAVLQETINSYAKGFRKDPS
                     HYYSGINAVALMHLLSHLTGKEENLEVRKEMEGGVRWAVRGALEKDSRDYWARATLAE
                     LEGLSNKKEVVDAALKSAVAVADRDWFKLNSSREQLLLYKDLGFRPVEVQAHLEIIDR
                     ALARAIRPEARWAPRQVFLFSGHMIDAPDRKDPRFPPDKEPIAAKAIADRLDALGAGA
                     EDLAICSGACGGDLLFAEACLARGLRLELRIPFDEPTFLRNSVAFAPGNWTDRFYQVK
                     GNPNTKLLVMPDELGPTPKEANPYARDNLWQLHAALSWGPDKVRFVCLWNRKGGDGPG
                     GTGHMYETVQKYSGRVYVLDTNLLW"
BASE COUNT          886 a          954 c         1184 g          843 t
ORIGIN      
        1 gggaaaagcc acggcgttca tgtggcaact ggcgccttac aatctttggg gtaatccgct
       61 caattgggac aaagtccgcg gtgagtggtt ccagaaacag ttggcggttt ggcagcgctt
      121 tgcgccgaac ctggacgaga ataatatcct gtcgacggat tccaccacgc ccctcgatat
      181 cgaacggcac gaccgcaaca tgtattgcgg cgattggatg gtgggcgagt actccggcga
      241 tcaagcgctg gagaaccggc cattccccgg ctggggaaac taccgaacgc cgatcgacgg
      301 tctttattta tgtggctcgt cctgtcatcc aggcggtaac atcaccggcg ctcccggtta
      361 caacgcggcg cgcgtcatcg ccgagtcgct cgggatcaag ccgtggtgga agccgctgga
      421 cttcaaagaa catatgaaag ggctgagata aaagaatgga gtattggagt accggagtat
      481 tggagtaatg ggctcaattt ccaccactcc attactccat cactccagca tcccaggggg
      541 tgccttatga ggctgggtgg taaggtcgcc atcgtcaccg gtggagcgcg gcacatcggc
      601 gccgcctatt gtcgcaggct cgccgtggag ggcgcggcgc ttgtcgtcgc cgatgttctc
      661 gacggcggaa gcgttgtaaa ggagattact acggagggtg gcaaggccat cgctttgaag
      721 gtcgatgttt ctcgggaaga agataccgcg cggatggcgg cggaagcgct gcaggcgttc
      781 ggccggatcg acatcctggt caacaacgcg gcgattttcc tcaacatcca acgccatccg
      841 ttctatgaaa tatccggtga ggagtgggac caagtctcgg ccgtcaacat taaaggtccg
      901 ttcttgtgcg ccaaggcggt ttttccccaa atgaagcaac agcggagcgg caagatcatc
      961 aacatttcat catctaccgt cttcgccggc acgccgttat ttttgcacta tgttacatcg
     1021 aaagctgcgc tggtcgggat gaccaggtcg ctcgcacgcg aagtgggtga attcggcatc
     1081 tgtgttaatg cgatcgcgcc gggattggtc caacacgaag gcatgaatgc gccgaaagag
     1141 ttcaccgagt ttcagttgaa aacgcgctca atcaagagac tgcaaacccc agaggatcta
     1201 ctcggcgttc tcgtctatct tgcgtcctcg gacagcgact ttgtcacggg ccagacgatc
     1261 ttgattgatg gcggcagcgt tttttattga attggtctgt gaataactct tatctcacgc
     1321 tttccatctt gcatgacggc tcaacgaaat tcgtcttcgg gcgaaacccg acggcaaccc
     1381 actagtaggc gacgcaaaat atggagcgga ccggtcgcgc tgttgctggt tgtggcagcg
     1441 ctctggtggc agtgggctca gactcagaag caatcccagg tcggcaaagc ggcgccggga
     1501 gttaactcgc taacagccgc agctgtctcc ggtatttggc gcggggaggt cacctatagt
     1561 gggggaacaa cctatacgga acgattcttg tttcaggtcg aaggtgataa actcttcgga
     1621 accgcgtctt ttctgggttt caagcgtggg atagaagatg gccgaataga tggcgacaaa
     1681 atttctttca acgtccggtt ccacgaagcg gtggaagggg tgagtacaga acgcacaaat
     1741 cgctatgccg gcagcgtttc cgacaaccag attcatttca agatgcaaga tgatcgaggt
     1801 gagcgaccgg tggagttcgt tgttagaaaa gagaaccggg cagagtaacg ggaggctgtt
     1861 gtggcatacc acgcgttcgt agcgatgccc ttcggcatca aagagcaaat agattttaat
     1921 agggtttacg gtgaactcat caagccggcg ctggaagacg ccggcttcga ggttttccgc
     1981 gcagacgagg agatgcgcgc gggggacatt cgcacggaca tgttccaaga gttgcttatg
     2041 gcggatctgg tcgtggcgga tctctccatc gataacccga acgtctggta cgagctcggc
     2101 gtgcgccatg cgctcagggc gcgcggagta atcggcatca tgtgccggcg caattacatg
     2161 ccgttcgatg tctacacgga ccgggtgcta acataccaaa taaaggacaa tcctccaggg
     2221 gctgccatcc cggatccgga gcgattggag gacggcaaga aaaaactcgc tgaatttgcc
     2281 acgcagacga tggattcctg gtacgagagg aaaatcagtc cggtgtatca cctgttgccc
     2341 gctctcaagg agccgcattt caagttgctg caagtcgaag gagcgaagga gttctggtcc
     2401 tgggccatgc gcatcgaggt cgcgagaaaa ggaaatcgcc cgggcgatat tctagtgttt
     2461 gcggaagaag ctccgaatat cggccttcgg gtcgaggcgt accggagtgc gggcaacggc
     2521 ctacgttctc taggacaatt tgctctggca ctcgatcaat acgaaaaggc gttggcgctg
     2581 gctcccgatg atctcgaaag tcgtcgtcag aagggtcttt tgttgggccg gctgaagcag
     2641 agcgacgcgg ccaaggaatg gctccaaaat ttggtaaagg aagttcctga cgatgccgag
     2701 agttgggcgc ttttaggccg cgtggaaaaa gatagttgga ttagtggttg gcgtgcggat
     2761 ggaaagacgg tggagcagat gcaagaagac gctgcggctg acgatgctgt gctccaggag
     2821 acgatcaatt cctatgccaa agggtttcgc aaagatccct cgcattacta ctcaggcatc
     2881 aacgctgtgg cgcttatgca tctactgtct catttaaccg gcaaggaaga aaacttggaa
     2941 gtacgaaagg aaatggaggg cggggtgcgc tgggccgtgc ggggggcgtt agaaaaggac
     3001 tccagagatt attgggcgcg cgccactctt gcggaattag aggggttgtc aaataagaaa
     3061 gaagttgttg acgcggcttt gaagagtgcg gttgctgtcg cagataggga ttggttcaag
     3121 ctgaattctt cacgtgagca gcttctccta tacaaggacc ttggttttcg gccggttgaa
     3181 gttcaagccc atctggagat tatagaccgg gcattggcgc gagcgatacg ccctgaggcg
     3241 cgatgggcgc cacggcaagt ttttctcttt agcggacata tgattgatgc gccggaccgc
     3301 aaagatccgc gttttcctcc cgacaaggag ccgattgcag ccaaagcgat cgccgataga
     3361 ctcgacgctc tcggcgccgg agcggaggat ctggctattt gcagtggcgc ctgcggcggc
     3421 gatttgcttt ttgccgaggc ttgccttgcg cgcggattga ggctggaact gcgcatcccc
     3481 ttcgacgagc cgacttttct tcgaaactcc gtcgcttttg ctccgggtaa ttggacggat
     3541 cgcttttatc aggtgaaggg taacccaaac accaagctgc tggtgatgcc ggatgaactc
     3601 ggtccaacgc ccaaagaagc caacccgtat gctcgggaca atctgtggca actacatgca
     3661 gccctctcct gggggcccga taaagttcgc tttgtttgtt tgtggaaccg gaaaggcggg
     3721 gacggtcccg gaggcacggg gcacatgtat gaaaccgtgc agaagtattc cgggcgggta
     3781 tacgttcttg atacaaacct tctttggtag tggaggagat gtttatgagt ctaacggaga
     3841 agatacaacc tgggagccag aaaaaaa
//
LOCUS       DASSWZ010000034         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_651893, whole genome shotgun sequence.
ACCESSION   DASSWZ010000034 DASSWZ010000000
VERSION     DASSWZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651893"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFN58_00770"
     CDS             complement(<1..442)
                     /locus_tag="VFN58_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8916548.1"
                     /translation="MNNARALIYVIDDDVSVRNGLDNLLRSAGFTVQTFSSAREFLAS
                     ARPDMPSCLLLDVQLPGLSGLDLQQELAKGGVEIPIIFITGHGDIPMSVRAMKAGALE
                     FLTKPVNDEDLLDAIQQAIARDHRARQPQGETGQSNFVGKSAALK"
     gene            complement(581..967)
                     /locus_tag="VFN58_00775"
     CDS             complement(581..967)
                     /locus_tag="VFN58_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8916549.1"
                     /translation="MSEFPLISIVDDDDSLRNSLNNLIRSVGFGVQGFSSAEAFLKSN
                     HLYDTACLILDVRMPGMSGLELQHQLAVTHCRIPIILITSHEDDGARARALEAGAVEF
                     LYKPFREEALLNAIHTALKSKEDGEN"
     gene            complement(1129..1752)
                     /locus_tag="VFN58_00780"
     CDS             complement(1129..1752)
                     /locus_tag="VFN58_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8916550.1"
                     /translation="MTEADAIVFVVDDDEPMRKSLQNLIGSVGLRVEAFASAPEFLRS
                     KLTDVPGCLVLDVRLPGLSGLDLQKRMVEAGIEIPIIFITGHGDIPMTVQAMKAGAVE
                     FLTKPFRDQDLLDAIQQALDRDRAARKQRTQINELHNRLDSLTPREREVMGLVVAGLL
                     NKQIAGELGTSETTIKIHRHQVMEKMGAGSLAELVRMADRLGIPTPK"
     gene            complement(1749..3311)
                     /locus_tag="VFN58_00785"
     CDS             complement(1749..3311)
                     /locus_tag="VFN58_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8916551.1"
                     /translation="MSKLGSLAERVPRAVWQYGLAVVSVTVALGITDWLEPYTTLRTP
                     LFYIAIISSAWFGGMGPGLLAVVLSTLAVGYYFAPGGQTPVLSLDTLPFLLLFSLSGL
                     IACWISVKRRRAEEALKRVRDELEVRVEERTSDLRRASEGLKAEIAERKRGEEALREK
                     ANLLDLTHDTIFVRDANNVITFWNRGAERLYGWKREEAVGQISHQLMQTIFPAPLEKI
                     MVELNSTGHWEGELIHALRDGTEVVVASRWALQLDERGKPIAILETNNDITERKRAEE
                     ALHRAQTELAHVTRVATLGEMTASIAHEVNQPLGAVVNNASACLRWLAGQAPNLEEAR
                     KSAALIIADGHRASEIISRIRALAKKAPPQKDWLDINETILEVIALARSEAQGNRIAL
                     QTQLSDDMPLILGDRIQLQQVILNLIINAIEAMSGVGEGPRELQVSSGKDDSQGALVA
                     VRDSGPGLDPKSLDHLFTAFFTTKPKGMGMGLAISRSIIEAHGGRLWATANQLRGATF
                     RFTLPADGERVS"
     gene            3665..4129
                     /locus_tag="VFN58_00790"
     CDS             3665..4129
                     /locus_tag="VFN58_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HET8916552.1"
                     /translation="MMTFLFAFFIGFFAGLRSLTAPAATAWAVHLGWLKLQGPLALMG
                     SIPAVTIFTLLAAVELVADKLPKTPKRTAPPGLIARIVMGGLTGACVATGGGQGALLG
                     AVLGAAGGVVGCFRGYEARTRLVSALGMPDIYVALLEDLVAIAGSLWVVSRF"
BASE COUNT          887 a         1247 c         1171 g          986 t
ORIGIN      
        1 ccttcagagc ggcactcttg cccacaaaat tggactgtcc agtctctccc tgcggctggc
       61 gcgctctatg atcgcgcgca atcgcctgct ggatggcatc cagtagatcc tcatcgttaa
      121 cgggcttggt gagaaactcc aacgcacctg ccttcatggc ccgcaccgac atcgggatat
      181 ccccatgacc ggtgataaag ataattggaa tctcaacccc gcccttggcc agctcctgct
      241 ggaggtcaag accgcttagg ccaggaagct gcacatccaa cagcaaacag ctgggcatat
      301 ccggccgcgc actcgccaaa aactcccggg ccgacgaaaa agtctgcacc gtaaagcccg
      361 ccgaccgcaa caaattgtcc agcccgttgc gaaccgatac atcgtcatct atgacatata
      421 tgagagcccg tgcgttattc atcgttttat ctcccgcttg ataaggccat taattgttga
      481 aacctaccaa tgcctgcccg ctgatcgcta gactccaaag ttcgcatcct attcgccaag
      541 gtttggcttt cgcctctttt aagaatgtct tgccctttat ctagttttct ccgtcctctt
      601 tagatttgag agctgtatga atcgcattta acagcgcctc ttcccggaag ggtttgtaca
      661 gaaactccac ggccccggct tcaagtgccc gcgctcgcgc gccatcatct tcatgtgagg
      721 tgataaggat aatcgggatt cgacagtgcg tgacagccag ctggtgctgg agctcgagtc
      781 cgctcattcc cggcatccgc acatcgagaa tcaggcacgc cgtatcgtac agatgattcg
      841 atttcaaaaa cgcttccgcc gaagagaaac cttgcacccc aaaaccgacg gatcgaatca
      901 gattattgag cgaatttctg agggagtcgt catcgtcgac aatcgaaatc agcggaaatt
      961 cggacatgca aacgcctttc ttagtcaatt ttccggaaac aagcctcatt caatgtactg
     1021 caaaagtgga tccacgttag cctttgtatt tcgaagatga tgatgccctc gaagacaatg
     1081 taccgcccac gggaaagagc gtctattgta ctttggtaaa ggcggctact actttggggt
     1141 agggatgccc agcctgtcag ccatccggac aagctcggcc agagagccgg ctcccatctt
     1201 ttccattacc tggtggcggt gaatcttgat cgtcgtctcg ctggtgccca gctctccggc
     1261 gatctgcttg ttgagcagcc cggcaaccac cagccccatc acctctcgct cacgcggcgt
     1321 cagcgagtcc aaccggttgt gcagttcatt aatctgcgtc cgctgcttgc gcgcagcgcg
     1381 gtcgcggtcc agcgcctgct gaatggcgtc gagcagatcc tggtcgcgga agggtttggt
     1441 cagaaactcc actgcaccgg ccttcatggc ctgcacggtc atcggaatgt ccccgtggcc
     1501 ggtgatgaag atgatgggaa tctcgatgcc ggcctcgacc atccgcttct gcagatcaag
     1561 cccgcttagg cccggcagcc tcacgtcgag cacgaggcag cccggcacat ccgtaagctt
     1621 gctgcgcagg aactctggag ccgaggcgaa ggcttcgacc cttagcccga cagacccgat
     1681 gaggttttgc agggatttgc gcataggctc atcatcatcc acgacgaata caatggcgtc
     1741 tgcttcagtc atgacaccct ctcaccgtca gccggcaacg taaaccgaaa cgttgcccct
     1801 cggagctggt tcgcggtcgc ccacagccgc ccgccgtgtg cctcgataat cgagcggcta
     1861 atggccagtc ccatccccat tcctttcggc ttggtcgtaa agaaagccgt gaaaagatgg
     1921 tcgagactct tcggatcgag ccccgggccc gaatcgcgca cggcgaccag cgcgccctgc
     1981 gagtcatcct tcccggagct gacctgcagc tccctcggcc cctcgccgac tccactcatg
     2041 gcttcgatgg cgttgatgat cagattcagg atcacttgtt gcaattggat tcgatctccc
     2101 agaatgagcg gcatgtcatc tgacaattga gtctgcagag cgatgcgatt tccttgcgct
     2161 tcgctgcgcg ccaaggcgat gacttcaagt atcgtttcgt tgatgtcgag ccagtccttc
     2221 tgcggcggag ctttcttggc gagggcacga atccggctga taatttcgct ggctcgatgg
     2281 ccgtctgcaa tgatgagcgc ggcagacttc cgggcttcct cgagattggg agcttggccc
     2341 gccaaccagc gcaaacaggc actggcgtta ttgaccaccg cgccgagtgg ctggttgact
     2401 tcgtgcgcaa tcgaggcagt catctctccg agagtcgcca cccgtgtgac atgcgccagt
     2461 tcggtctgtg ccctgtgtag cgcttcctcg gcgcgcttgc gctcggtgat gtcattgtta
     2521 gtctccagga tcgcgatggg ctttccccgt tcgtccaatt gcagcgccca ccggctcgcg
     2581 actacgacct ccgtcccgtc gcggagagcg tggatcaatt caccctccca gtggcccgtg
     2641 ctatttagct ctaccatgat cttttccaac ggcgcaggga agattgtctg cataagctgg
     2701 tgagagatct gtccgactgc ctcttctctt ttccacccgt acagtctctc ggcgccgcga
     2761 ttccagaaag taatgacgtt gttggcatcg cgtacgaata ttgtatcgtg agtgagatcc
     2821 agtaggtttg ccttttcacg caaagcctct tcgccccgtt tgcgttccgc gatttcggcc
     2881 tttaaccctt cactggcccg ccggaggtcg gaggtccgtt cctcaactct tacttccaat
     2941 tcatcgcgca cccgcttgag cgcctcctcc gccctcctcc gtttaacact tatccaacag
     3001 gcgatgaggc ctgacaatga gaacaggagg aggaagggca gggtgtcgag gcttaaaact
     3061 ggcgtttggc cgccaggggc aaagtagtaa cctactgcca gcgtagagag cactacggca
     3121 agcaaccctg gtcccattcc gccgaaccaa gcactgctga ttattgctat gtaaaagagc
     3181 ggcgttcgga gtgtggtgta gggctccagc caatccgtga tgcccagcgc cactgtgacg
     3241 ctcaccacgg ccaacccgta ttgccaaacc gcccgaggaa ctctctcggc caaagagcct
     3301 aatttggaca tgccttcacc ggatgaatca gagatccttc tcactatatt gctcgagaga
     3361 aagggcagag acgaaccttt cttcataaga ttctcattca gctcgagtag tggcaagacc
     3421 ctataaaaag gcagagaagc agtttcgtct gaagtattaa aaaattgttg agcatcgcag
     3481 agtgtcccgg gcagaccgaa gtaatgaaac aaactggtaa caattctgtg accgtattac
     3541 ttaatcggtg aattcggcac atttttgctt gcaaacggaa ctggcagggg agaaaactgg
     3601 gcaagaacgg gcggctgtaa gcgatgtcgg tggactttaa atcgacggac ccgaggagga
     3661 ctgcatgatg acgtttctct ttgccttttt cattggattc tttgcgggat tgcgctcgct
     3721 tacggctccg gcggcgacgg catgggcggt ccacctgggc tggctgaagc tccagggtcc
     3781 acttgctctc atgggttcga ttcctgccgt cacaattttc accctgctcg cagctgtgga
     3841 gttggtagcc gacaagctgc cgaaaacccc gaagcgcacg gcgcccccgg gcctgatcgc
     3901 acgtattgta atgggtgggt tgacaggcgc gtgcgttgct actggcgggg ggcagggagc
     3961 gcttcttggc gccgtgctgg gtgctgccgg cggcgttgtg ggttgtttca gagggtacga
     4021 agcacgcact cggctcgtga gcgcactggg tatgcccgac atctacgttg ccctgctgga
     4081 ggatctagtg gcgatcgctg gctcgctgtg ggttgtttca cgattttgag cttcgctgtg
     4141 ggctttacgt tagcgactca gggccgagaa aaattttcca cgcagatctg gaaggcggcg
     4201 gccgtgagtt gctcgatgga gcaccaaaaa agtggccagg tttgctcggt tatctgaggg
     4261 atagcgggat tgcccacgtt gtagtagtcg a
//
LOCUS       DASSWZ010000035         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_878380, whole genome shotgun sequence.
ACCESSION   DASSWZ010000035 DASSWZ010000000
VERSION     DASSWZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878380"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="tgt"
                     /locus_tag="VFN58_00795"
     CDS             <1..793
                     /gene="tgt"
                     /locus_tag="VFN58_00795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET8916553.1"
                     /translation="LSEEGVRFQSHLDGSYHLLTPENVVQIQEALGSDIAMVLDECIP
                     HDANREYARDSTARTIRWAERCLRARSQADQLMFGIIQGGMYEDLREQCVREMNSMPF
                     DGFAVGGLGVGEGEELLHSIAASTAGLLPKTRPRYLMGVGRPEDIMRAVRAGFDLFDC
                     VLPTRNARNGTLFTAQGKLSIKRAEFASDRRPLDETCDCYCCRNFSRAYLRHLYVAGE
                     ILSSQLNSLHNLYFYHRLMERCREAIRQERTDFWSREAVAFSQPV"
     gene            812..1147
                     /gene="yajC"
                     /locus_tag="VFN58_00800"
     CDS             812..1147
                     /gene="yajC"
                     /locus_tag="VFN58_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET8916554.1"
                     /translation="MWDIAYAQGAPGLTGPSPVMTILPFILIFVIMYFLVIRPQQKKG
                     KEHQEMLNKLKKNDEVMTSGGIYGKVVALADNVVTLEVAPNVRIRVHRPQISAVVTGE
                     KSSSKEVKE"
     gene            1152..>2129
                     /locus_tag="VFN58_00805"
     CDS             1152..>2129
                     /locus_tag="VFN58_00805"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET8916555.1"
                     /translation="MRRSVMIRLALFVVATLVSLVYLAPTFVHPLPAWWSGFLPTDRI
                     HLGLDLQGGSHLLLEVKVDKAIENNVERVKTDLTNVLRDRGISGVSVERVQSTQLQVK
                     VPAASVERVRGLLKSDFANLTVANTQTGGGMTDFFLTLSKEELRSLRDYAVDQSLETI
                     RNRIDQFGVSEPVIQREGQQEILIQLPGIQDPERAKEIIGKTASLEFKLVDDAANVEE
                     AVRNGPPPGRQILYGYVGKGEGGVGDKTPYVLESRTLMTGEYITDARVRPSSQLQGPY
                     VELILNSTGARLFEQITAANVKRRLAIVLDNRVYSAPVIQERIGGGRASI"
BASE COUNT          472 a          540 c          640 g          477 t
ORIGIN      
        1 actatcggaa gagggggttc gctttcaatc tcatctggac ggctcttatc atctccttac
       61 cccagagaac gtcgttcaga ttcaagaggc cctcggaagc gacatcgcga tggtgctgga
      121 cgaatgtatc ccccacgacg cgaaccggga gtatgctcgg gattccacgg cgagaacgat
      181 ccgttgggct gaacgttgtt tgcgagcgcg aagccaagca gatcaattga tgttcgggat
      241 tattcaaggt ggtatgtatg aggatcttcg agagcagtgt gttcgggaaa tgaattccat
      301 gcccttcgac ggctttgctg tcggcggttt gggcgtgggt gaaggggagg agttacttca
      361 ttccatcgcg gcatctacgg ccggcttgct gcccaaaact cggccacgct atctcatggg
      421 tgtgggcaga cccgaggata ttatgcgtgc cgtccgcgcc ggttttgatc tgttcgattg
      481 cgtcctgccg acgcgtaacg ctcgaaacgg cacgctcttt accgcgcaag gtaagctcag
      541 tatcaagcgc gccgaattcg ccagcgatcg gcggccgctg gatgaaacct gcgactgcta
      601 ctgctgtcgc aacttctctc gggcgtactt gcgccacctg tacgtcgccg gagaaatcct
      661 ttcatcccag cttaacagtt tgcacaatct ttacttttac catcggctca tggaaaggtg
      721 tcgtgaggcc attcgccagg agcgtaccga tttctggagc cgagaggccg ttgctttttc
      781 acagccggtc tagaatttga caggaggaaa catgtgggac attgcctatg cccaaggggc
      841 gccgggactg acggggccca gcccggtgat gacgatatta ccgttcattt tgatcttcgt
      901 catcatgtat tttttggtga ttcgaccgca gcaaaagaag ggtaaagagc atcaggaaat
      961 gctcaacaag ctcaaaaaaa atgacgaggt gatgacctcg ggaggaattt acggtaaggt
     1021 ggtggccctg gcggataacg tggtgacttt ggaggtggct cccaacgtga ggatccgtgt
     1081 tcatcggccg cagatctcgg cggttgttac cggcgagaag agttccagca aagaagtaaa
     1141 ggagtaaggg aatgcgacgg agcgtgatga ttcggctggc actgtttgtt gtcgcgaccc
     1201 tcgtttcgct agtgtatctg gcacccacgt ttgtgcaccc tctgcccgct tggtggagcg
     1261 gctttttacc gacagaccgg attcacttag gactagatct tcaaggcggc agccatcttc
     1321 ttctcgaagt gaaggtggat aaagcgatcg agaacaatgt cgagcgcgtc aaaaccgatc
     1381 tcaccaacgt cctacgtgac cgaggcatct cgggcgtatc ggttgaaagg gtacagagca
     1441 ctcagctcca ggtcaaagtt ccagcggcca gtgtggagcg ggtgcgaggg ttgttgaaaa
     1501 gcgatttcgc gaatctcacc gtggccaata cgcaaaccgg gggggggatg acggattttt
     1561 tcttgacgct cagtaaggag gaactccgtt ccctgcgcga ctatgccgta gaccagtcgt
     1621 tggagaccat tcgcaatcgt attgaccaat ttggagtgag cgagccagtc atccagcgcg
     1681 agggacagca agaaatcctt atccagctcc ccggcattca agatcccgag agggcgaagg
     1741 agatcatcgg caagacggcg tcactcgaat ttaagttagt cgatgacgcc gctaacgttg
     1801 aagaggctgt tcggaacgga ccaccgcctg gtcgccaaat tctatacggc tacgtgggca
     1861 agggagaggg tggtgtgggg gacaagactc cctacgtgtt ggagtcccgg acattgatga
     1921 ccggcgaata cattaccgat gcgcgcgtgc ggccgagctc gcaattgcag ggaccttacg
     1981 tagagctcat cctcaattct accggcgccc ggcttttcga gcagatcacg gcggcgaatg
     2041 ttaagcggcg gctggccatc gtgctcgaca atcgagttta ttcagcgccg gtgattcagg
     2101 aacgaatcgg cggcggcagg gcttccatc
//
LOCUS       DASSWZ010000036         7935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_722842, whole genome shotgun sequence.
ACCESSION   DASSWZ010000036 DASSWZ010000000
VERSION     DASSWZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722842"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..169
                     /locus_tag="VFN58_00810"
     CDS             11..169
                     /locus_tag="VFN58_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1722 domain-containing protein"
                     /protein_id="HET8916556.1"
                     /translation="MGHIIDFRHNPVPLVVPLTLVRHYVNKFDVGYLQRQVYLQPSLK
                     ELLLLNHV"
     gene            203..679
                     /locus_tag="VFN58_00815"
     CDS             203..679
                     /locus_tag="VFN58_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8916557.1"
                     /translation="MRTYLLPTSLWLPQPREQIFAFFSDPKNLEKITPPWLRFKSLSP
                     TELAVGRGTRIDYRLRVRRLPIRWQSEITVWEPPIRFVDQQTRGPYKAWVHEHTFSES
                     EGGTIAGDKVVYAVPGGKLVQKFLVRPDLDRIFQYRHEVLQSIFNPTGYDAQTRWT"
     gene            732..2117
                     /locus_tag="VFN58_00820"
     CDS             732..2117
                     /locus_tag="VFN58_00820"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET8916558.1"
                     /translation="MDSLERIADDPRVRIRRAGLNAQAKCVLYWMRRSQRGLDNAALN
                     VAVQAGNVLRKPVMVFFGLTPKAHHANLRHYTFLMQGLHDVAQDLRKRNVGFVLRRDP
                     EHHILKLCSEVQPCLVVGDENPLRESERTQARVAAEVKAPFWSVDSDVIVPTKLLGKE
                     HYAARTIRPKIRALLVQFLKPLHNPTAKLIWQKEPTLVDNSLLEKLPLDRAVQPVSAF
                     VGGSMEASRRLKEFLQFRLSGYAMRRNKPDLDGTSQLSPYLHFGHIAPHVVAMAVQEA
                     DAALQDRTAFLEELIVRRELAVNFVRYNSNYENFDSCEAWADVTLRKHAGDKRRFLYT
                     EKQLQNAETHDPLWNAAQKQMVLTGWMHGYLRMYWAKKILEWSPSPAVAYDIAVRLND
                     RYELDGRDPNGYAGIAWAIVGKHDRAWGPERPVYGKVRYMSYDSTSRKFNSKAYIERI
                     ALLEKNRLAAS"
     gene            2283..3446
                     /locus_tag="VFN58_00825"
     CDS             2283..3446
                     /locus_tag="VFN58_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8916559.1"
                     /translation="MVLWFLGALQIILGARVILRLIRSVGGVRIEAATARPSERVSII
                     LPVLNETARIKACLDSLVAQSDAVAEILVVDGGSSDGTQAIVESYGLQDRRVRLIDAS
                     PVPCDWAGKTWGLYVGLKNTDPRSHWVLFIDADVRCSPLLAPSLLAHAERTGISTFSV
                     ATHQQLSSLAEGLLHPSLLTTLVYRFGIPGKATRNRHQVQANGQCFMSLRDTLLRTDA
                     FRAAQLSLCEDFTMARRLAECGEATGFYESDGLVKTGMYQGYCETWRNWPRSLSTRDR
                     YFGWHEATGLLEILLVQSLPLPLLSVLYAVGASGWIVAVNLALTVTRLGALMGVARAY
                     EWRPWTYWFSPLLALPVGLRLITSALRRRQYGGAEFICDAPEEFLSRWTDPRP"
     gene            complement(3632..3895)
                     /locus_tag="VFN58_00830"
     CDS             complement(3632..3895)
                     /locus_tag="VFN58_00830"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET8916560.1"
                     /translation="MAKKDQPDKKFESALEELEGVVEQLESGDLSLEDALAAFEKGVG
                     LVKYCNQKLNEVEKKVELLLKDKEGRLQLKLLDSLAGDEEKED"
     gene            complement(3898..4818)
                     /locus_tag="VFN58_00835"
     CDS             complement(3898..4818)
                     /locus_tag="VFN58_00835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET8916561.1"
                     /translation="MKLVRILGIGIALLPVFDSYSFGRPAAKTSATKPIEISQGDIVE
                     LKIPGIGLTTVEGRMGKAMVPFFSSGTGDYTAFVGADLEAKPGLVTVTVKGTTTTGLQ
                     RDSQMTLLIKPKPFKKESFSVGAEFDQLSPEVLERIRKDQDEFSRTFMTSAPDRLWEG
                     PFLLPISSEVSSPFGYRRVINGAPRAPHTGVDLRAALGSEVVAANHGRVVLLGDFFFS
                     GHSLVLDHGGGLYTMYFHLSGFKVEMGAAVRKGDVIGLSGMTGRVTGPHLHWGARING
                     ARVDPFELVNKLGNSQSRLESDATGDGAKR"
     gene            complement(4820..6049)
                     /gene="xseA"
                     /locus_tag="VFN58_00840"
     CDS             complement(4820..6049)
                     /gene="xseA"
                     /locus_tag="VFN58_00840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET8916562.1"
                     /translation="MKKAADDLPLFARQAPRFFTISELNQIIKGTLEQELDGLWVVGE
                     VSNFRVPPSGHYYFTLKDDKSQILAVMFRRQGVALSFTPENGMEVLCFGKVSLYPARG
                     DLQLYVESMEPRGQGTLYLAFEQLKRRLSAEGLFAEERKRALPFLPVSIGIVTSEKGA
                     ALRDMLRIIGDRYPDRRIVVWPVKVQGVGAAEEIAKGITELGRSGEVDVMIVGRGGGS
                     LEDLWAFNEEIVARSISMTPVPVISAVGHEIDFTIADFAADLRAPTPTAAAEIAVPRK
                     SELVGQVMDLEEQLARCMWRRLELAKENWRGFVKRLADPERKLRETQQRVDELSVDLV
                     RRFRDRMRQVKDRFVQGGGRLDALSPLAVLDRGYSIVHKIPEEKLVKDTTSLDLGDFL
                     RVTFAKGKAICRVERKE"
     gene            complement(6155..7525)
                     /locus_tag="VFN58_00845"
     CDS             complement(6155..7525)
                     /locus_tag="VFN58_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET8916563.1"
                     /translation="MRIVAKRGFPVILLLVGMALGFFLAAQWVPSVSAVPRQDYESLE
                     AFTNILSIVKKNYVEDVDTKNLVSGAINGMLSSLDPHSAYLTPELYKELQMDTQGRFG
                     GLGIEITVKGGILTVVSPIEDTPAAKAGVKPGDQIFKIEDEFTKDMTLVDAVKKMRGP
                     KGSKITISIKREGVPELINFTLMRDTIRVQSVRSRNLEEGYGYIRLAQFQERSDRDLQ
                     KALEKMVAEKAGLKGLVLDLRNNPGGLLTQAVRVADLFLDSGMIVYTEGRIESQKQKY
                     FAQKDGSWMDFPVVVLVNGGSASASEIVAGALQDHKRAVVLGTKTFGKGSVQTILPLD
                     DNSALRLTTARYFTPKGRSIQAMGIVPDIIVESAPPQEAKADEKRRQSLREENLPGHL
                     QNQQNQQQTPNSQPEPGDKEKEKQIPSSGPTGDDSIDNDAQLKRALDLLKSWDVFKQL
                     VQKKAA"
     gene            complement(7595..>7935)
                     /locus_tag="VFN58_00850"
     CDS             complement(7595..>7935)
                     /locus_tag="VFN58_00850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET8916564.1"
                     /translation="KIRHPEFSAEVFRKGIDIEAPIGEEIRAVEKGRVVFAERFSGYG
                     RMVIIDHGERYFSVYAHLSEIIKKIGDTVRRGETLGRVGDSDSLAGSRLYFEMRKDGK
                     SIDPLPWFRK"
BASE COUNT         1806 a         2299 c         1956 g         1874 t
ORIGIN      
        1 ggaggaactc ataggacaca tcatcgattt tcgacacaac cctgtgcctc ttgtcgtccc
       61 actgaccttg gttcggcatt atgtcaacaa attcgacgtg ggctatctgc aaagacaggt
      121 ttacctacaa cccagtctaa aggagctctt gctgctcaat catgtgtaga attcaacaag
      181 ctctgagcca gcgcaaatca taatgagaac ttacctacta ccaaccagtc tttggctgcc
      241 acagccccgg gagcagattt ttgctttctt ttccgatcca aagaatcttg aaaagatcac
      301 tcctccatgg ctccgtttca aaagcctcag cccaaccgaa ctcgccgtcg ggcgcggtac
      361 acggatcgac tatcggctgc gtgttcgacg cttgccgatt cgttggcaga gcgaaatcac
      421 ggtgtgggag ccgccgatac gcttcgttga ccaacaaacc cgaggtccct acaaagcgtg
      481 ggtgcatgaa cacacttttt cagaatccga gggaggaaca atcgctggcg ataaggtggt
      541 ctatgcagta ccgggaggaa agctggttca aaaattcttg gtccggccgg acctcgatag
      601 aattttccag taccgtcacg aagtcctgca aagcatcttc aatccaacgg gttacgacgc
      661 tcagacccgc tggacttaat taatcctcga cagcgacgtt tccaaagttt tgggcgttga
      721 aaactgattc tatggactct cttgaacgta ttgctgatga tccgcgagta agaattagaa
      781 gagcgggctt aaacgcgcag gcgaaatgtg tcctctactg gatgcggcgc tcgcaaagag
      841 ggctggataa tgcagcgctc aatgtggcgg tacaagctgg aaacgttctc aggaagccgg
      901 tcatggtgtt tttcggtctg accccgaagg cgcatcacgc caatctcagg cactacacat
      961 tcttgatgca aggtctccac gatgtcgctc aagacctacg gaaacggaac gtcggttttg
     1021 tgttgcggcg tgaccccgag caccacatcc taaagctttg ctccgaagtt cagccctgcc
     1081 tcgttgtcgg ggatgaaaac cccttgcgcg agtcggagcg cacccaagcg agggtggcgg
     1141 cagaagttaa agcccctttc tggagcgtcg actcagacgt gatcgtaccc acgaaactcc
     1201 tcggtaagga gcattacgca gcgagaacca tacggccaaa gatccgcgcg ctgctggtcc
     1261 aattcttaaa acccctccac aatccgaccg ctaaacttat ttggcagaag gaacccaccc
     1321 tcgtggacaa ttccctattg gaaaaattgc cccttgatcg tgccgttcaa ccggtctccg
     1381 ccttcgtcgg cgggtcaatg gaggcaagtc gaagacttaa ggagtttctg caatttcgac
     1441 tcagtggcta cgccatgcgc agaaacaagc ccgatctcga tggcactagc cagctttcac
     1501 cttacctcca ctttggacac atcgcgcccc acgtcgtcgc catggccgtc caagaggctg
     1561 atgctgcgct tcaagaccgg acagcgtttc ttgaggaatt gatcgtgcgc agagaactcg
     1621 cggtcaactt tgtccgctac aactcgaact atgaaaactt tgactcctgc gaagcctggg
     1681 cggatgtcac gctccgtaag cacgcagggg acaagcgccg tttcctctac accgagaaac
     1741 agttacagaa cgcggaaacc catgacccat tgtggaacgc ggcgcaaaaa caaatggtgc
     1801 ttaccggttg gatgcacggt tatctacgaa tgtattgggc caaaaagatc ctcgaatgga
     1861 gtccatctcc cgcagtcgct tacgatatcg ctgtgcgact aaatgatcgt tatgaactgg
     1921 atgggcgtga tccgaacggc tacgccggca tcgcctgggc gattgttgga aagcatgatc
     1981 gtgcctgggg accggagcga ccggtatacg gcaaagtccg ttacatgtcc tatgacagca
     2041 cgtcgcggaa attcaacagc aaggcttaca ttgaacgaat cgcgcttctg gaaaaaaatc
     2101 gcttagcggc aagttgaatt cgtctagccc atgttgcgcg gccattcggt tctcaatgca
     2161 gagtcatcga gcctggtcga attgacgcca agaactcgat gctcaagctg cgaacttcga
     2221 tgccaccgaa aagacagact ccaattacag gcaagaagct ctcaacgtaa gcgaaagccg
     2281 atatggtcct ttggtttcta ggcgcgctgc agatcatcct tggtgctcgc gtcatactgc
     2341 gtctaatccg aagcgtaggc ggggtaagga ttgaggccgc tacggcgcgg ccatccgagc
     2401 gcgtcagcat catcttgccg gtcctcaacg aaaccgcgag aattaaagct tgtcttgaca
     2461 gtctcgtggc tcaatcagac gcagtggcag aaatcctggt agtggacggc ggttccagcg
     2521 acggcaccca agccatcgtt gagagctacg gattacagga ccgacgcgta cggttgatcg
     2581 acgccagccc cgttccctgc gactgggccg gaaaaacgtg gggcctatac gtggggttga
     2641 aaaacaccga tccacggtca cactgggttc tttttatcga tgcggatgta cgctgctcgc
     2701 ctctgctggc accttccttg ttggcccatg ccgaacgaac cggaatttca actttctcgg
     2761 ttgcgaccca ccaacaactt tccagtctgg cggagggcct cctccaccct agtcttttaa
     2821 cgaccctggt gtatcgcttc ggcataccgg gaaaagccac acgcaaccga catcaggttc
     2881 aagcgaacgg ccagtgcttc atgagtctgc gcgacacctt gctaagaacc gatgcattcc
     2941 gcgcggctca actatccttg tgcgaagatt tcacgatggc tcgacgcctt gctgaatgcg
     3001 gcgaagccac cggtttctac gaaagcgacg gactcgttaa gacgggaatg taccagggct
     3061 attgtgagac ttggcgcaat tggcccagat cgttatccac ccgtgatcgg tacttcggct
     3121 ggcatgaagc tacaggtctg ttggagattt tgttggttca atcgttgcca ttgccccttc
     3181 tgtctgtcct ctatgcagtg ggcgcctcag gctggatcgt cgccgtcaac ttagcactaa
     3241 ctgtcactcg tctcggcgcc ttaatgggcg tcgccagggc atacgaatgg agaccatgga
     3301 catattggtt ttcgccgctg ctcgcccttc cagtgggcct acgactgatc acgagtgctc
     3361 tccggcgtcg tcagtatggc ggggccgagt ttatttgcga cgcgccggag gaatttttga
     3421 gccgttggac agatccaaga ccgtaagcca atagtccttc ggcttgcaac ttttgcctat
     3481 aactttgttt gatcgaataa cctgccgagc catacggctt agcgcccgga cattcgctgc
     3541 aggacgtgaa agggttgtga gccggcggtc aatcatgaaa aacaattcgt acatcgcttg
     3601 ggacagcacc gtgatgcctc tatcactcgc cctaatcctc cttttcctca tcaccggcca
     3661 gagaatccaa tagtttgagt tgcagcctgc cctctttgtc cttgagcaaa agctcaactt
     3721 ttttttctac ttcattcaat ttctgattac agtacttgac cagacccact cccttttcaa
     3781 atgcggccag cgcatcttcg agtgaaagat cccccgattc caattgttcg acgactcctt
     3841 ccagctcttc cagagccgac tcgaatttct tgtccggctg atctttcttg gccattttta
     3901 tctcttcgct ccatcgccgg tggcatcgct ttcgagtcgg ctctgagaat tgcctagctt
     3961 gttgaccaat tcaaacgggt caacacgcgc gccgttgatc cgggcacccc aatggaggtg
     4021 cggccctgtc actcgcccag tcatccccga taacccgatc acatccccct tgcgcaccgc
     4081 cgctcccatt tcaactttga atcccgagag atggaaatac atggtgtaaa gtccaccccc
     4141 gtggtccagc accaggctat ggccgctgaa aaagaaatcg ccgagcaaca cgactcgacc
     4201 gtggtttgcg gcaaccactt ctgagcctaa cgctgctctt aaatcaactc cggtatgcgg
     4261 tgcacgtggc gcgccgttga tcacgcggcg ataaccaaag ggagagctaa cttcgctgga
     4321 aatgggaagc agaaagggcc cttcccatag acggtctggc gcggaagtca tgaacgtgcg
     4381 ggaaaattcg tcttggtcct tccgaatcct ttccagtact tccgggctca actggtcgaa
     4441 ttcagcgcca accgaaaacg attccttttt aaagggcttt ggcttgatca gcaaggtcat
     4501 ctggctgtcg cgctgtagcc ccgtcgttgt cgtacctttg acggtgaccg ttactaagcc
     4561 gggcttggct tcgagatccg ctccgacgaa tgccgtataa tcaccggttc cgctcgaaaa
     4621 gaaagggacc atcgcttttc ccatgcgtcc ctcgacggtg gtcaaaccaa tgccgggaat
     4681 tttcaactcg acgatgtcac cttgagagat ttcaattggc ttggttgccg aggttttggc
     4741 tgccggacga ccgaaggagt aactatcgaa gaccggcagc agtgcaattc caatccctag
     4801 gatacgcacc agtttcatct cattcctttc tctccacccg gcagatcgct ttgcccttgg
     4861 caaaggtaac tcggagaaaa tcgccgaggt ccaaagaggt tgtgtccttg acgagctttt
     4921 cctccggtat tttgtggacg atgctgtaac cacgatccaa aaccgccaag ggacttaacg
     4981 catccagccg cccgcccccc tgaacgaacc ggtccttgac ctgacgcatg cgatctcgaa
     5041 aacggcgcac gaggtcaact gataactcat ccacgcgctg ttgcgtttca cgcaacttcc
     5101 gctcgggatc ggccagtctc ttgacgaatc cgcgccagtt ctctttggcc aactccaatc
     5161 tccgccacat acatctcgcc agctgctctt ccagatccat cacctgcccc accaattcag
     5221 actttctcgg aacagcaatc tcggcggccg cggttggtgt gggcgcccgc aaatccgccg
     5281 cgaagtcagc aatggtgaaa tctatttcgt ggcccaccgc ggagatcacc ggcaccggcg
     5341 tcatggagat gcttcgcgcc acgatctcct cgttgaaggc ccagaggtct tccaaggacc
     5401 caccgccgcg gccgacgatc atgacatcca cctcacccga ccgaccgagc tcggttatcc
     5461 ccttggcaat ttcttcggcc gctcctacac cctgaacttt caccggccac acaacgatgc
     5521 ggcgatcagg ataacggtcg ccgatgatgc gcaacatgtc ccttagagcg gcaccttttt
     5581 ccgaggtcac aatgccgatc gagacaggca agaacggcaa cgctcgcttg cgctcctctg
     5641 cgaataatcc ctcggcagac agtcttcttt tcaactgctc gaaggccaag tagagggtcc
     5701 cttgccccct tggctccata ctctcaacat acagctgcaa atctccacgc gccgggtaga
     5761 gactgacttt gccgaagcac aggacctcca taccattttc gggggtgaag gaaagagcaa
     5821 caccctggcg tcgaaacatc acggccaaaa tctggctctt gtcatctttg agcgtgaagt
     5881 aatagtggcc ggaaggggga acacgaaaat tagagacctc accaacgacc cataatccgt
     5941 ccaactcttg ctcgagggta cccttgatga tttggttgag ctcgctgatg gtgaaaaacc
     6001 gtggcgcttg tcgggcgaac agaggtaagt catccgccgc ttttttcatt agggtccagc
     6061 tccggctgct gaatgtctag ctacaaaaaa ttagccctcc ggacggaggg ctaatagaga
     6121 aattagcttg agaagtttgc cgaccgcacc cggttcaggc cgctttcttt tgcaccagct
     6181 gtttaaagac atcccaactc ttcagcaagt ccaaagcccg cttaagctgc gcatcattgt
     6241 cgatgctgtc gtcacccgtc ggtccagagg agggtatctg tttttctttc tctttgtctc
     6301 ctggctccgg ttgcgaattg ggggtctgct gttgattctg ttgattctgc agatgacccg
     6361 gcaggttttc ctcgcgcaag ctctgcctgc gcttttcatc ggccttcgcc tcctgcggtg
     6421 gcgcgctttc cacgatgata tccggcacga tacccatcgc ctgaatcgat cgtcccttgg
     6481 gcgtaaagta acgtgcggta gtcaagcgca atgcggaatt atcatccaag ggtaaaatcg
     6541 tctggaccga acccttgccg aaagttttcg tccccaatac aaccgcgcgt ttgtgatcct
     6601 gtaacgcccc agcaacaatc tcagaggcgc tggcactccc cccgttcacg agaaccacaa
     6661 cggggaaatc catccacgag ccgtctttct gcgcgaagta tttctgcttt tgcgactcaa
     6721 ttcgaccctc cgtgtaaaca atcatgcccg agtcgagaaa tagatctgca acgcgcacag
     6781 cctgggtgag taagccccca gggttgtttc tgaggtccaa aaccaatccc ttgagacctg
     6841 ccttttcagc gaccattttt tcaagagcct tctgaagatc gcgatcactt cgttcctgaa
     6901 actgcgcgag ccgaatgtag ccgtatcctt cttcaagatt tcggctgcgc acgctctgga
     6961 cacgaatcgt atcgcgcatc aaagtaaagt tgataagctc cggcacacct tctcgcttta
     7021 tagagatggt gatcttgctc cccttcggtc cgcgcatttt ctttactgcg tccacgaggg
     7081 tcatgtcttt ggtgaattca tcttcgatct tgaagatctg atctccaggc ttcacgccgg
     7141 ctttagcggc aggcgtatct tcgatcggcg ataccacggt gaggatcccg cctttaaccg
     7201 tgatttcaat tccgagacca ccgaagcgtc cctgtgtatc catttgcagt tctttgtaga
     7261 gttcaggcgt gagataagca ctgtgtggat cgagggaact cagcattcca ttgatggccc
     7321 cgctgactag gttcttcgtg tccacgtcct ctacatagtt cttcttaacg atggaaagaa
     7381 tattggtaaa agcctctaga ctctcataat cctgtcgggg aacggccgaa acactcggca
     7441 cccactgagc tgctagaaaa aatccgagcg ccatcccaac gagcagcagg atgaccggga
     7501 acccgcgctt tgctacaatt cgcatagtct ctctcctcgc tgaagtccaa acattcagtt
     7561 tggttattct atcccaacac tttgagaata tctattattt tcgaaaccat ggcaaggggt
     7621 cgatggactt gccatccttg cgcatctcga agtaaagcct tgagccggcg agggaatcac
     7681 tgtcgccaac ccgtcccagg gtctcgcctc gccttactgt gtcaccaatt ttcttaataa
     7741 tttctgatag atgcgcgtaa acactaaagt aacgctctcc atgatcaatg atcaccatcc
     7801 tgccatagcc cgaaaaccgc tccgcgaaca ctaccctgcc cttctctact gctctaattt
     7861 cctcaccgat tggggcttca atgtcaattc cttttcgaaa cacttcggct gaaaactcgg
     7921 gatgtctaat tttgc
//
LOCUS       DASSWZ010000037         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_156807, whole genome shotgun sequence.
ACCESSION   DASSWZ010000037 DASSWZ010000000
VERSION     DASSWZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156807"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFN58_00855"
     CDS             complement(<1..657)
                     /locus_tag="VFN58_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916565.1"
                     /translation="MRAIDVHVHPSTRGLDCDACAYFRRELNEVPTTEEKFAELFVTQ
                     QVKALLIGWHPSTVKDGTRNSNEHVIELVTKYPAAFAGVLASLNVGSQPVVELIQKAE
                     TLLKNPAVKGFKFHPPDQNFYPDDRKHYEVWEILETGQKPVMFHTGFTVLGANQDGGQ
                     GIGLDCGRPIHLDTLARDFPRMKIIAAHPGWPWQEELIGVVTHKKNIHVDTSGYLAEQ
                     L"
     gene            complement(644..1852)
                     /locus_tag="VFN58_00860"
     CDS             complement(644..1852)
                     /locus_tag="VFN58_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET8916566.1"
                     /translation="MIDGINKVGVVGLGVMGFDIAFLYAMRGCRTFVYDAAKVAMDSL
                     PGRSEQTIERLKRRKRISDDQIESVRNGLIGAVGLKELAKMDLVTEAVSESAKTKLAV
                     YQALREAGFAGILTTNTSSLARSFLLAGGVPDRSKFALTHFFNPVLYTQMVEVVKGDM
                     ETTNFDTVVSFLKRLGREPVETKDISGFVSNSILMYYAVMALQLVQCGARIEDVDQAA
                     KELRVLPPFISFDNWKPSIVEDVTQVMFELRGDEFLRSSKLLAALAKDNPKFYIDQKP
                     NPAIYRLVETPNREIGVASIKRALKTVIHMAAARVVELGESPAIVDFVATEGVKIPQA
                     PLEEMDSLGAGAVLQELSALNQEMQEISLTPPELLAAMAREKQPFFRDGQPNPWLVSL
                     VKSPTTHASY"
     gene            2025..>2348
                     /locus_tag="VFN58_00865"
     CDS             2025..>2348
                     /locus_tag="VFN58_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916567.1"
                     /translation="MTLGKASTIVLLGLYLSIAGLNRFAIAAEPDKINVCYSSIAATS
                     ITTWVPKEAGIYKKYGLDVSIIYVAGAQAITTLISGDTQIVQGSGAAAALSRLSGSDL
                     NVIATT"
BASE COUNT          532 a          654 c          595 g          567 t
ORIGIN      
        1 cagctgctcc gccaaatagc cggacgtgtc cacatggata tttttcttgt gtgtcaccac
       61 gccgatgagt tcctcctgcc agggccagcc cgggtgagcg gcgatgattt tcatccgtgg
      121 aaagtctcgc gccagcgtat cgagatggat cggccgaccg caatccaggc cgattccctg
      181 gccgccgtcc tgattggccc ccagaaccgt aaatcccgta tgaaacatga cgggcttttg
      241 ccccgtctcc aatatttccc acacctcata atgctttcta tcgtcgggat agaagttttg
      301 gtccggcgga tgaaacttga agcccttcac tgcgggattt tttaacagcg tctcggcctt
      361 ttggataagt tcaaccacag gctgggatcc cacgtttagc gatgccagaa ctccggcaaa
      421 ggcggccgga tacttcgtga ccagctcaat gacatgctca ttgctgtttc gcgttccgtc
      481 cttcacggtg gacggatgcc agccgataag cagcgctttg acttgctgcg tgacaaataa
      541 ctccgcgaac ttttcttctg tggtgggaac ctcgttcagc tctctcctga aataagcaca
      601 cgcgtcgcag tccaggccgc gagtgctggg atgaacatga acgtcaatag ctcgcatgag
      661 tggttggcga ctttactagg gagacaagcc aggggttggg ttggccgtca cgaaaaaaag
      721 gttgtttttc acgagccatg gcggcaagaa gctcaggcgg agtcaaagat atttcctgca
      781 tctcttggtt aagcgctgat aactcttgaa gtactgcgcc agctccaagg ctgtccattt
      841 cttccaacgg cgcctgggga atttttactc cctccgtggc gacaaaatcc acgatggcgg
      901 gcgattctcc aagctctacg actctcgccg ccgccatatg gatcacggtt ttcagagcgc
      961 gcttgatgct cgctacgcct atttcccggt tgggagtttc gactaatcga tagatcgccg
     1021 gattgggttt ttgatctatg tagaactttg ggttgtcctt tgccaaggcg gcgagcaatt
     1081 ttgacgagcg cagaaattca tcgcccctga gctcgaacat cacctgggtg acatcttcta
     1141 cgatggatgg tttccaattg tcgaaactga tgaaaggagg aagtactcgc agctctttcg
     1201 ccgcctgatc aacgtcctcg atgcgcgctc cacattgaac cagctgaagc gccatgacgg
     1261 cgtagtacat gagaatgctg ttggaaacga agcccgatat atctttggtt tcgacgggtt
     1321 cacgtcctaa gcgtttaagg aatgaaacca cggtatcgaa gtttgtagtt tccatgtccc
     1381 ctttcacgac ctcgaccatc tgggtataga gaaccgggtt gaagaagtga gtaagggcga
     1441 attttgatcg gtcgggaacg ccgccagcga gcaaaaacga cctcgccagg gatgacgtat
     1501 tagttgtaag gatgcccgca aatcctgcct ctcgcagagc ctgatagacc gccagcttcg
     1561 tctttgcgct ttccgaaacc gcttccgtca ccagatccat ttttgccagc tcttttagtc
     1621 caaccgcacc aatgaggcca ttgcgaacgc tctctatttg atcatcggaa attcgcttcc
     1681 tcctcttgag ccgttcgatg gtctgctcgc tgcggccagg taaagagtcc atcgccactt
     1741 tcgcggcatc gtagacaaac gttcggcagc ctcgcatggc gtacaagaaa gctatatcga
     1801 accccatgac gccgagcccg actacgccga ctttgtttat tccgtcaatc atgagacttc
     1861 gctcaaaagc attcgacttt acccgagata ccataggcga tgggtcaact caatgtcatt
     1921 caaaatattc gccttgactt agcggtaacc cttttctaag gtcaccgacg aacggcttaa
     1981 aaacaaacag ggcccaaggc aatcgacaat ggagggagta ttcgatgacg ctcggtaaag
     2041 catcaaccat cgtgctctta ggactttatc tctcgatagc cggcttgaat cgtttcgcta
     2101 tcgccgccga gcccgacaag atcaacgttt gttattcttc catcgcggcg acctcgatta
     2161 ccacctgggt gccgaaagag gccgggatct acaagaagta tggccttgac gtgagcatca
     2221 tctatgtcgc gggagcgcag gcgataacga ccctcatttc cggcgacacc caaatcgtac
     2281 aaggctccgg cgccgccgcg gcgctctcgc gcctatccgg ctccgatcta aacgtgatag
     2341 caacaacg
//
LOCUS       DASSWZ010000038         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_284302, whole genome shotgun sequence.
ACCESSION   DASSWZ010000038 DASSWZ010000000
VERSION     DASSWZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284302"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1192
                     /locus_tag="VFN58_00870"
     CDS             158..1192
                     /locus_tag="VFN58_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916568.1"
                     /translation="MAKALGVFLLPSFISLSLVTAALAASVEEFYKGKTIQFIVGGSA
                     GGGYDTYTRLIARYFTQYVPGKPSTVVQNMPGAAMLIAANYVFNSAPRDGTVIGHWSG
                     PLILQHMMGNPAVQFEGRKFGWLGMPTADSLICITTDRSGIKSAEDWRKAKTRVKLGA
                     IGPGTSGTDDTKLLAAATGFPLQLIEGYKGTADIRIAAEQGEVDGTCAFGWQSAKVTW
                     ANALRARQVHVVLQTMTEPHPELKGVPLAVEYANTEEGKKLLRIAGDLYGKQRLYSLP
                     PQVPEERVRTLQKAFINTLKDSQFLAEAEKAKLEIDPVDGPGIEKMVNGLYEIEPAIV
                     NRVKQLLESK"
     gene            1289..1672
                     /locus_tag="VFN58_00875"
     CDS             1289..1672
                     /locus_tag="VFN58_00875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8916569.1"
                     /translation="MPKKEVIQVDLAAANPNLSPATRFGNLVFVAGQTGRHPTSGEVG
                     KGVREQTHYALQRVKTILEAAGTSLDNVLTVTTHLTTREDLAAYNEEYAKFFPINKPA
                     RTTVEVMLNSPELLVEITVTACIPD"
BASE COUNT          483 a          565 c          575 g          441 t
ORIGIN      
        1 ggaccagcga tagatctcgt aaaggccgtc aacggtctca agctattggg ccgctagaat
       61 gatgcgatga tgaattcaat tcctgtgaac cgcttgactt gacctcgcgg gcaactatag
      121 cttccagcag aacggacaaa atacaaggag gaggttcatg gcgaaggcac tcggcgtatt
      181 tttgcttcct agcttcatct cgttatcgct tgtcaccgcg gcgctcgctg cctcggtcga
      241 ggagttctac aagggcaaaa ccatccaatt catcgtcggt ggctccgccg gtggaggcta
      301 tgatacgtac accaggctta tcgcgcgcta cttcactcaa tacgttccgg gtaaaccctc
      361 gactgtcgtc caaaacatgc ctggagcggc tatgctgatc gcggcgaact acgtctttaa
      421 cagcgcgccg cgcgacggaa cggtcatcgg gcattggtcc ggcccgctca ttctacagca
      481 catgatgggg aaccctgcgg tgcaatttga ggggcgcaag tttggctggc tcggtatgcc
      541 gacggcggat tcccttatat gcataacgac tgatcgcagc gggataaagt cggcagagga
      601 ttggcgcaag gctaaaacac gggtcaagct gggagcgatt ggccccggca ccagcggaac
      661 cgatgatacc aagctcctcg ccgccgctac gggtttccct ttgcagctca tcgagggcta
      721 caagggtacc gccgacatca gaattgcagc ggaacaaggg gaagtcgacg gtacgtgcgc
      781 ctttggctgg caatcggcga aggtgacttg ggccaacgcc ctgcgggcac gacaggttca
      841 tgtcgtgctc cagacgatga cggaacctca tcccgagctg aagggtgttc ctcttgctgt
      901 cgagtacgcc aacacggagg aagggaaaaa actcctccgg atagccggcg acctttacgg
      961 caaacaaaga ctctattctc ttccgccaca agtgcccgaa gagcgcgtgc gaacactgca
     1021 gaaagccttc atcaatactc tgaaagattc tcagtttctg gctgaggcag aaaaggcgaa
     1081 gctcgagatc gatccggtcg atggacccgg catagaaaag atggtgaacg gcttgtatga
     1141 aatcgagccc gccatcgtga atcgagtgaa acagttactg gagtcaaagt aggagtgaga
     1201 gaacttctac taaacggaag aaggctaggg ataaaaagtt aaacgggctc gacgactcgc
     1261 ggagcgttcg gtccgacagg agatggagat gcctaagaaa gaagtgattc aggtagatct
     1321 cgctgcggcc aatcccaatc tgtctcccgc tacccggttc ggcaatctgg tctttgtggc
     1381 cgggcagacg gggagacacc cgacttcggg ggaagttggt aaaggcgttc gcgagcagac
     1441 ccattacgca ctgcagcgtg tcaaaacgat cttggaagcc gccggcacat ccctcgataa
     1501 cgtcctgacc gtgacaaccc atctcaccac acgggaagac ctggcggctt acaacgagga
     1561 gtacgccaag ttcttcccga tcaacaagcc ggccaggacc accgtcgaag tgatgctaaa
     1621 ctcgcccgaa ctcctggtgg agatcaccgt aaccgcttgc atccccgatt gatcctggtc
     1681 tgtgaagtgg gggattgtta cgctcccaac ccctttgatt tcttctccgc tacgtacttt
     1741 ttcaaagcaa ggcgcctctt ttgtctcttg cgcctatgcg aattttttcg tgcgtcctgc
     1801 ttcattgcta gctccttgtg cgccggaaca aaccggcggg ttgtttgcgg atcagagtcc
     1861 cacacggcga atcgttagcc acgccggact tgagtcatgc gtggctatgc tttcgaatag
     1921 tgacactttc tgttcccgtc tgctagagga ttttgtgtca aggcgaccgc gaactcacgc
     1981 ttgccggagg tttctcgccc gctccgtgac aacaaataaa acggtttgcc tgtgtctcaa
     2041 attcagtaca gaacatggca agca
//
LOCUS       DASSWZ010000039         9927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1020000, whole genome shotgun sequence.
ACCESSION   DASSWZ010000039 DASSWZ010000000
VERSION     DASSWZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020000"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="VFN58_00880"
     CDS             complement(<1..1732)
                     /locus_tag="VFN58_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8916570.1"
                     /translation="MPLLRLDGVSLAYGHLPLLAQIDLQIEPGERICLVGRNGAGKTT
                     LLRVVTGVALPDEGDIWRHDTLRIAHLEQEVPPDTEETIYEVVAAGLGELGELLTEYH
                     QVTHEAGTGERPSLQRMAKLQARIDALGGWNISQKVETVLTRLGLPADQSLASCSGGI
                     RRQTMLARALVSDPDLLLLDEPTNHLDITAITWLENFLLAYRGALIFITHDRMFLKHL
                     ATRIIELDRGKLSSFPGDFDAYLSKKEELLEIETRATANFDKKLAEEEAWIRQGIKAR
                     RTRNEGRVRVLQAMRRERSQRLELPGQVRFGLDAGVLSGKLVVDLRRVSFGYGDNVII
                     RGLSTTILRGDRIGVIGANGCGKSTLLKLILGEIAPTSGEVVMGTRLKLAYFDQHRRE
                     LNPDKTVRENMTHGSDYVAVQGRSRHVIGYLRDFLFPPERIDSPVQALSGGERNRLLL
                     AKIFTQSANMLVLDEPTNDLDVETLELLEDLLADYQGTLLLVSHDRTLLDNVVTSTLV
                     FEGEGKVGEYAGGYEDWKLQRAAAPLRELPKPSAPPLVAAAVTEKTNNRNRKLSYKER
                     RELEALPGKIE"
     gene            complement(1829..2260)
                     /locus_tag="VFN58_00885"
     CDS             complement(1829..2260)
                     /locus_tag="VFN58_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET8916571.1"
                     /translation="MASGDWDSVMWQRASDLLQQVERIHRNFLQIAAGVQYRSSHGRS
                     PAWEPPVNVVETDESLWVISALPGVATDRMNVRLEGRELLIAGERPLPKCCNDGELKI
                     WEIPLGRFERRLTLIDGREALSLGEVSLHDGLLIIELRKAL"
     gene            complement(2444..3007)
                     /locus_tag="VFN58_00890"
     CDS             complement(2444..3007)
                     /locus_tag="VFN58_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916572.1"
                     /translation="MKLIKNGVSSEMVGHEVQRITSNLIDEGLVWREAIDFVKGRIRN
                     GVGAVFSTTNYHEGAAGFLHALRQKGWIQQTELEKICLCGSKIDWSSRIVGHFNMGSN
                     KVIGLADALRIGEEALKAGISFVFGDDPLGNDRELLEIAPYAYVIKNEKNRHLNLPDR
                     VRLVTWSEVQAMSISELVSLHTDPGEK"
     gene            complement(3870..6323)
                     /locus_tag="VFN58_00895"
     CDS             complement(3870..6323)
                     /locus_tag="VFN58_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET8916573.1"
                     /translation="MIDPICGMEVEPSKAAGSHVHNGKTYSFCSHHCLAKFKEDPEKF
                     LKSSAAGHVAHGYEHARAHAAPQIPKTDKAEGGIYLCPMDPEVRESKPGVCPKCGMAL
                     EPAAASATAVKTEYVCPMHPEIVRPEPGFCPICGMALEPRTVTLEEEANPELVDMTRR
                     FWVGVVLSVPIALLAMSDMIPGQPVQRIVSPQLLNWLQLVLATPVVLWCGWPFFQRGW
                     ASIVNRSLNMFTLIAIGVGTAYIYSVVATFFPQLFPESFRGHSGEVGVYFDAAAVITT
                     LVLLGQVLELRARSKTSKAIKALLGLAPKTARLIRDDGTEVDVPLERVKPGDRLRVRP
                     GEKIPVDGIVLEGTTSVDESMISGEPIPAEKTSGSRVTGGTVNGTGSFIMRAERVGSD
                     TLLAQIVRMVAEAQRSRAPIQKLADVVAGYFVPAVVLVAVITFIVWALFGPEPPMAYA
                     LVNAVAVLIIACPCALGLATPMSIMVGTGRGAIAGVLIKNAEALEVLEKIDTLVVDKT
                     GTLTEGKPRLISVIPARERDEGELLYLAASLERGSEHPLAAAIVSGAEQRGIKFTDAR
                     EFRSITGRGVVGSVDGKRVALGNRKLLEELHVGAGDLWNRSEELSRDGQTVMYVVVEG
                     TVAGLLSVADPVKPSTPEAIRMLHEDGIKIVMLTGDNRTTAEAVAKKLAIDEVHAEVL
                     PEQKGNLVKRLQAEGRMVAMAGDGVNDAPALAQAHVGIAMGTGTDVAMESAGVTLVKG
                     DLRGIAKARRLSRGTMRNIRENLFFAFIYNILGIPIAAGVLYPFFGMLLSPVIASVAM
                     TFSSVSVITNALRLNRLQL"
     gene            complement(6544..7224)
                     /locus_tag="VFN58_00900"
     CDS             complement(6544..7224)
                     /locus_tag="VFN58_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET8916574.1"
                     /translation="MTIPQVALPETLPVFPLTGVLLLPGTVLPLHLFEPRYRAMVEDT
                     LSADRVFGMIQPFAPQQDNRPMPGAENQRPDLYKVGCAGYIEKWEKLPEGRFFVELRG
                     VNRFRFEKELSLQRGYRRVLADYQTFPDRKLYEGWQCDRSAVLQALASYGQAHGLQVK
                     PEQAERFNDLELVNLLSVSLPFHPAEKQALLEAATLKDREKVLVNLLRLGSGPDDPGS
                     DIPPRTVN"
     gene            7428..7859
                     /locus_tag="VFN58_00905"
     CDS             7428..7859
                     /locus_tag="VFN58_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET8916575.1"
                     /translation="MAEFKSVSIDSAKDFLTKHHWGVLATRRKDGRLQMSPITPGLDG
                     DGRVIISSRETAYKVNNLRRDPRAALCAFTTSFHGEGWVQVNGSAEIISLPDAMNSLA
                     YLQQQVYGEHKSWPEFRARMVRERRVIIRFAIESVAPLRRG"
     gene            7916..8524
                     /locus_tag="VFN58_00910"
     CDS             7916..8524
                     /locus_tag="VFN58_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET8916576.1"
                     /translation="MDWRNWLLSFFLLFALSILLIETHAAEQSDDLTGQLLVATPEMR
                     DPRFVETVIYMVKHSGEGAFGLVINRPLAKGPVEDLLKGFGIDSRGAKGEIIIHYGGP
                     VSPRAGFVLHSDDVLLEESAKVTNGIAMTSDPKLIEAMGQGKGPRQSLFVLGYAGWAP
                     GQLEGELLAGSWFVVSGDKALIFGQDADRKWRQAMDKRKIPL"
     gene            8692..9624
                     /locus_tag="VFN58_00915"
     CDS             8692..9624
                     /locus_tag="VFN58_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916577.1"
                     /translation="MLRIAVPDLVSNSYFPIIAAVELGCFKSEGFDASVDLLFPVPKT
                     FEALRDGQLDFVVGSAHATLLAFPDWKGAKLLCAIGKHTYWFLVIRSDLNPKRGDLSV
                     VKGLRVGAAPGVNLSLQRLLVEAGIDPEKNNVQIVPIPGAAGPNVSFGLSAAKALEEG
                     KLDGFWANGMGCEVALRRGVGSMVLDVRRGDGPPEARNYTFSALVATESRIAKEPDSA
                     RAAIRAVMKAHKALKEDVNRATEVGRKRFPPSEAELIAELIRRDLPYYDPTITEETVT
                     HMNRFAQDIGLLSAPVAYDQVVATQFTHLWKEPV"
BASE COUNT         2288 a         2862 c         2545 g         2232 t
ORIGIN      
        1 cttcgatttt tcccggcagt gcttctagct cgcgccgctc cttgtaagaa agtttacgat
       61 tcctgttgtt ggtcttttct gtaaccgcag ccgcaaccag tggcggcgca ctgggttttg
      121 gcagctccct tagcggcgcg gccgcgcgct ggagcttcca atcctcgtac ccgccggcat
      181 attctcccac cttaccttcc ccctcaaaaa ccaaagtgct cgtcaccacg ttatcgagaa
      241 gggtgcggtc atgactcacc agcagcaagg tcccttggta atcggccagc aggtcttcca
      301 atagttcgag cgtctccacg tctaaatcgt tggtcggctc atccagcacc agcatgttcg
      361 ccgactgcgt aaaaatcttt gccagcaaca aacgattgcg ctcgcccccg gacaaggcct
      421 gcaccggcga gtcgatgcgc tcgggcggaa aaaggaagtc tcttaggtag ccgatgacgt
      481 ggcgggaacg tccttggacc gcaacgtagt cggagccgtg ggtcatgttt tcgcgcactg
      541 tcttgtcggg atttaattct ctgcggtgtt gatcgaagta tgcgagcttc aggcgggttc
      601 ccatgaccac ctccccgctc gtcggtgcta tctcgcccaa gatcagcttg aggagtgtgc
      661 tcttaccgca gccgttggca ccgatgacac cgatccgatc gccgcgcagt atagtcgtcg
      721 ataggccgcg aatgataaca ttatcgccgt aaccaaaact cactcggcgc aggtcgacca
      781 ctagtttgcc ggacagtaca ccggcatcga gcccgaagcg gacttggccc ggcagttcca
      841 accgttggct gcgctcccgc cgcatggcct gcaagaccct cacccgccct tcgttgcgcg
      901 tgcgtcgggc cttgatgccc tggcgaatcc acgcttcttc ttccgccagc ttcttgtcga
      961 agttagccgt cgcgcgcgtc tcgatctcca gcagttcttc ttttttactc aagtaagcgt
     1021 cgaagtcgcc gggaaaagaa ctcaactttc cccgatcgag ttcgatgatg cgggtcgcca
     1081 aatgctttag aaacatgcga tcgtgggtga taaagatcag cgcgccgcgg taagcgagta
     1141 aaaaattctc cagccaggta atcgcggtga tgtcgagatg gttggtcggc tcgtccaaca
     1201 gcaacagatc cgggtcactc accaatgccc gcgccagcat ggtttgtctt cggattcctc
     1261 cggaacagct agcaaggctc tggtccgcgg gcaaccccaa ccgcgtcaag accgtctcta
     1321 ctttctgact gatgttccag ccgcccaaag catcgatgcg cgcctgcaac ttcgccatcc
     1381 gctgtaggga agggcgttcc cccgtacctg cctcgtgtgt cacctggtgg tactccgtca
     1441 gcaactcgcc cagttcgccc aatcccgcgg ccaccacttc atatatcgtc tcctcagtat
     1501 ccggaggcac ctcttgctcg aggtgagcga tgcgcaacgt atcatggcgc cagatatcac
     1561 cctcgtccgg gagcgccacc ccggtcacaa cgcgcagcaa tgtggtcttg ccggcgccat
     1621 tgcgaccgac aaggcaaatc cgttcacccg gctctatttg aagatcaatc tgagcgagca
     1681 acggcaaatg gccataagcc agagatacac cgtcgagtcg aaggagaggc atagtaacta
     1741 agccgtaggc gacaaatcta tataaccgac gccccggcag gagtgataaa gcgaggtttg
     1801 gactcaggga cttccttctc tactgaagtc atagagcctt cctaagctcg atgatgagca
     1861 gcccgtcatg gagcgagact tctcccaaag ataaagcctc tcttccgtcg atcaaagtga
     1921 ggcgccgctc gaaacgtcca agcgggatct cccatatctt taattccccg tcattacagc
     1981 atttcggcaa gggccgttcg ccggcgatca aaagctcgcg tccctccaag cgcacgttca
     2041 tgcgatcagt ggcgactccc gggagggctg aaatcaccca caaactttcg tcggtctcga
     2101 ccacgttcac aggcggctcc caagcggggc tcctcccgtg ggaagaacga tactgaacac
     2161 cggcggcaat ttgcaaaaaa ttcctgtgga ttcgctccac ttgctggagc agatcactgg
     2221 ctcgctgcca catcacacta tcccaatcgc cggaggccat attaatcact cctcaactga
     2281 tgaatacaat gactcgttat cacagaccct ctaaaagcct aggattcgaa cgcaccattg
     2341 tcaagaaagt aacccggaaa ctgtcgcata gagaatgaca aagatgtcaa gctgtcaacg
     2401 acatgtccat gaaagtggca tgtggccacg acatgactag gatctatttc tcgccagggt
     2461 cagtatgcag acttaccaat tcggagatag acattgcttg aacctccgac caggtaacca
     2521 accttacgcg atcgggcaaa ttcaaatggc gatttttttc atttttaatg acataggcat
     2581 aaggagcaat ctccaatagc tctcgatcgt tgcccaacgg gtcatcccca aaaacaaaac
     2641 ttatcccagc cttcaatgcc tcttcaccga ttcttaaggc gtctgccagg ccaataactt
     2701 tgttgctacc catattaaaa tgaccaacaa tcctggagct ccaatcaatt tttgaaccac
     2761 acaggcaaat cttttccaac tccgtttgct gaatccaacc tttttgccgc aaagcatgga
     2821 gaaacccagc agctccttca tgataattcg tggttgaaaa gactgcccca accccgttgc
     2881 gtatccggcc tttcacaaaa tcaatcgcct ccctccagac taatccctcg tcaattaaat
     2941 tgcttgttat ccgctggact tcatggccaa ccatctctga gctgacacca ttcttgatga
     3001 gcttcaagcc tcttttcatc attgtgagag aacactcaaa aggatccatc ccactctgaa
     3061 tttcggtctt ccattcggca acatacctat caaataaatc tttgtccgcg gatagactcc
     3121 gtgtggacag ataccagaga tcgtggcctg gaatggtcag tgttccgtca atatcataga
     3181 catcgaaatg ataggcttgc attcgatcag ccgggaatgt ctgactagta gtatactgat
     3241 tcagagcaat tccaaagtct atcgtttatc tattattgtt tttcctctgt aagcacgtcg
     3301 gcagcaagat cccgacgatt cgcggaattg ttcgaggcag gcttcgaggt cttcgacaat
     3361 ttcctgggcg agaacgtcgg gattcggaag attgtcggat gctttcaagc gtttcgtctc
     3421 tgctaggaga gctgagatta ggaaagaccc ccctttgttc ccctccaggg ccggacatga
     3481 agagagcgca gcctcacagc acgaaccccg tctcgcgagg tttcacgctc aacatcgacg
     3541 tggaacgatg ttgaaaacgt agagccacaa acgttgatgc aaagaactag ccgctagatg
     3601 accgccggcg aatttcaaaa tatcaaacat tgggctcaag ccgctgtcta acttagaaga
     3661 caatcgccca gttgccagga atttgtcaag atatttgatt ggacatgaaa atgaaactcc
     3721 ggataaatga aaagcggaat gacgcgtgga aaacaacccc gatcggggat tctgcttcgt
     3781 gatgaacttc gacgctcata cccttctcat taccgcgctg ctcgttcctt cacggaatcc
     3841 gatcttccgt ttcgctcaga gtgtctaact taaagctgca gtctgttcag cctcagtgcg
     3901 ttggtgatca cggagacaga gctgaaggtc atggcgacgc tggcgatcac cggacttagc
     3961 agcatgccaa aaaaaggata aagcacgccc gcggcgatgg ggattcccag tatgttatag
     4021 ataaacgcaa agaagagatt ttcgcggatg ttccgcatgg ttccacggct gaggcggcgg
     4081 gctttggcga taccgcgcaa atcacctttg accaaagtga cgccggcgct ctccatcgcg
     4141 acgtcggtgc cggtgcccat ggctattccc acatgagcct gggccagcgc cggtgcatcg
     4201 ttcacgccat cgcccgccat cgccaccatc cgtccctccg cctgcaaccg cttgaccaag
     4261 tttccctttt gctctggcaa tacctcggcg tgaacttcat cgatagccag cttctttgcg
     4321 accgcttctg ccgttgtgcg attgtctccc gtcagcatca cgatctttat cccatcttca
     4381 tggagcatcc ggatcgcttc aggcgtggac ggcttgaccg ggtcggcaac gctcagcagt
     4441 ccggccaccg ttccttcgac gacgacatac atcaccgtct gcccgtctct gctaagctct
     4501 tccgaccgat tccacaaatc acccgcgcca acatgcaatt cttccaagag tttgcggttg
     4561 ccgagcgcga ctcgctttcc gtcgacagaa ccgaccacac ctcttcccgt gattgagcga
     4621 aactctcgcg catcggtaaa ctttattcct ctctgctcgg caccgcttac aatggctgcc
     4681 gccaacggat gctcgctgcc tcgctcgaga ctggctgcga gatataagag ttcgccttcg
     4741 tctcgctcgc gcgccggaat cacggagatc aaacggggtt tgccctcggt caaggttcct
     4801 gtcttgtcca ccaccaacgt gtcgattttc tccaggacct ccaacgcttc ggcgttcttg
     4861 atcagcacgc cggcgatcgc gcctcgcccc gtaccgacca tgatcgacat cggcgtggcc
     4921 aagccaagcg cgcagggaca ggcaatgatc aacaccgcca cggcattgac caaggcataa
     4981 gccatgggag gctccggccc aaatagagcc caaacgatga aggttataac ggcgaccaga
     5041 accaccgccg gaacaaaata gccggccaca acgtctgcca atttctggat cggtgcacgg
     5101 ctgcgctgcg cttccgcgac catgcgcacg atctgcgcga gcagagtgtc gctgccgaca
     5161 cgctccgccc gcataatgaa gctgccggtt ccgttgaccg ttcctcctgt cacccgacta
     5221 ccgctagtct tctcagcggg aataggctcg ccgctgatca tagattcatc cacagatgtt
     5281 gtcccctcaa gaacgattcc gtccaccgga atcttctctc ccgggcgaac ccggaggcgg
     5341 tctccaggct tgactcgctc cagtggtacg tctacttccg tgccgtcatc tcggatcaga
     5401 cgagccgtct tgggcgcaag ccccagtagc gccttaatcg ctttgctcgt cttgctgcgg
     5461 gcgcgtagtt ccaatacctg accgagcaga accagcgttg taataaccgc tgcagcgtca
     5521 aagtaaacac cgacttcgcc actgtggccc cggaaagatt cggggaagag ctgcgggaag
     5581 aaagttgcaa cgacactgta gatataggcg gttccgacac cgattgcgat aagcgtgaac
     5641 atattcaggc tgcgattgac aatggatgcc caaccccttt gaaaaaaagg ccatccgcac
     5701 cagagaacca ccggtgttgc caaaacaagt tggagccaat taagaagttg gggagaaaca
     5761 atccgttgca cgggctgccc gggaatcatg tcggacatgg cgagaagtgc aatcggcaca
     5821 ctcagcacaa cgccaaccca aaaccgccgc gtcatgtcga ccaattccgg attggcttct
     5881 tcctccaaag ttacggttct gggttcgagg gccatgccgc atatgggaca gaaaccgggc
     5941 tccgggcgga cgatctccgg atgcatcgga cacacgtact ctgtctttac ggctgtagcg
     6001 gatgcagccg ctggttccag agccatacca cacttggggc aaacgcccgg cttggactca
     6061 cgcacctcgg gatccatcgg acacaggtag attccgcctt ctgctttatc ggttttggga
     6121 atctgcggag ccgcgtgtgc gcgcgcatgc tcgtacccgt gagcaacatg cccagcagcg
     6181 ctcgacttta gaaatttctc tgggtcttct ttaaatttcg ccagacagtg atgactgcaa
     6241 aaagaatagg tttttccgtt gtggacgtgg cttcctgcag ccttgctggg ctccacttcc
     6301 atgccgcaaa tcggatcaat cattacctaa ctccgcaacc tgtaaattat cacgcccagc
     6361 tccatgttcc gagcgctata gatgaacaat tgccaaaatc tgcgaaacca atctaatttc
     6421 atgctccgta gattgaacta cttgtccgca cgtgccgaag tgcgcgatcg gtgcgcagaa
     6481 gcgggacgtt gggctcgtta gccgacaccg gttagcgtca acaactgaag ttttatctta
     6541 ttttcaatta accgtgcgcg gtgggatgtc tgaaccggga tcgtcgggac cgctgccgag
     6601 tcggagcaaa ttcacgagga ctttctcgcg atctttgagc gtggcggctt cgagcagcgc
     6661 ctgcttctcg gcgggatgaa acggtagcga cacactcaaa agattcacca gctcaagatc
     6721 attaaagcgc tctgcctgtt ccggtttcac ctgcaggccg tgagcttggc cgtaacttgc
     6781 gagcgcttga agtacggccg aacgatcaca ttgccagccc tcataaagct tccgatcagg
     6841 aaaagtttga tagtcagcta agacgcgccg ataaccgcgt tgcagcgaaa gttctttctc
     6901 gaaccgaaac cggttaactc cccttagttc cacgaagaac cggccttccg gtagtttttc
     6961 ccacttctcg atgtagccag cacaaccgac tttgtagagg tccggtcttt gattttcagc
     7021 gcccggcatg ggccggttgt cctgttgcgg ggcaaacggc tgaatcattc cgaaaacacg
     7081 atccgcactg agcgtgtcct caaccatagc gcgataacgc ggctcgaata ggtgcaacgg
     7141 caaaaccgtt cccggcaaga gcaagactcc ggtcaaggga aaaaccggta gcgtttccgg
     7201 caacgccact tggggaatcg tcatcgccac ttttgtctcc ttgcaactct ataacgcatc
     7261 ggattaacta ccgcaatgcg cgaaagttca agacagttgg caaatgatct cgttttacta
     7321 atcgtccaag actacgaaat tcggatcggc tcttgcaatc tatcattggg gtattctagt
     7381 ttggcgatga cttaaaatgc agacgatttc tgcccgttag aaacactatg gctgaattta
     7441 aatcagtgag tatcgacagc gcgaaagatt ttctgacaaa gcatcactgg ggcgttcttg
     7501 cgacccggag aaaagacggc cggctgcaga tgtcgccgat aaccccggga ctggatggtg
     7561 atggccgggt gatcatcagc agccgggaaa ccgcatacaa ggtcaacaac ttgcggcgtg
     7621 atccgcgggc cgcgctttgc gctttcacca cctcctttca cggcgaaggt tgggttcaag
     7681 tcaatggcag cgcggaaatc atttctcttc cggacgccat gaattcgttg gcctatttgc
     7741 agcaacaagt ctatggcgaa cataaaagct ggcccgagtt ccgcgcgaga atggtccgcg
     7801 aacgccgggt aattattcgc tttgccatcg agagcgtagc gccgctgcga cgaggataaa
     7861 tgtggctcga agcggtcctg ctcacggagt tatcttgcag aggtatcggc aatttatgga
     7921 ttggcgaaac tggttgctaa gctttttttt gcttttcgcc ttgagcatcc tgctaatcga
     7981 aacccatgcc gccgagcaaa gcgatgatct cactggacaa ttactcgtgg cgactcctga
     8041 aatgagagac ccgcgatttg tcgaaacggt catatacatg gtgaaacaca gcggcgaagg
     8101 cgccttcggt ctggtcatca accgcccgtt ggcgaaaggc cccgtagaag acttgctcaa
     8161 agggtttggc atagatagcc gcggcgcgaa gggtgagatc atcatccatt acggcggccc
     8221 cgtcagtcca cgcgcgggct ttgtgcttca tagtgatgat gtcctcctcg aagagtccgc
     8281 caaggttaca aatgggattg cgatgacatc ggaccccaag ctcatagagg cgatggggca
     8341 gggaaaagga ccacggcaat cgttgttcgt tttgggttat gcaggttggg cgccgggcca
     8401 gctcgaagga gaactgttgg ccggttcctg gtttgtggtt tctggagata aagcgctgat
     8461 ctttggtcag gacgcagatc ggaaatggcg tcaagcgatg gacaaacgaa aaattccgct
     8521 gtgagctgtg ggacgggagg ttcgattctg tcccggacga ttcggttgtc atcccgagca
     8581 aacgcgaggg atctaggatt tctcgctccg ctcgaaatga cacgagaact caacagatct
     8641 ccgcacacac atttgcgact aaaaacttta ctttgcacag gagagtttta aatgttacgc
     8701 atcgcggttc ccgatctggt ttcgaattct tatttcccca tcattgcagc cgtagagtta
     8761 ggctgcttca aatctgaggg cttcgatgca tcggtagatc tgctgtttcc cgtacccaaa
     8821 actttcgaag ccttgcgcga tggccagctg gatttcgtcg ttggctcggc acacgcaacg
     8881 ttgttggcat tcccggattg gaaaggcgcg aagctgctct gcgccatcgg caagcacacc
     8941 tactggtttc tcgtcattcg cagcgatctt aatcccaagc gcggagactt gagcgtagtt
     9001 aaaggactgc gtgtcggcgc ggctccgggc gtgaatctca gcttgcagcg tttgctggtc
     9061 gaggccggca tcgatccgga aaagaacaat gtgcagattg taccgattcc cggcgcggca
     9121 ggaccgaatg tttccttcgg cctctcagcg gcaaaggcgc tcgaagaagg caagctcgac
     9181 ggcttctggg ccaacggcat gggctgcgaa gtcgcgctgc gccgcggcgt cggcagcatg
     9241 gtcctggatg ttcgccgcgg tgacgggcct cctgaggcgc ggaattatac tttctccgcg
     9301 ctcgtggcga ccgagagtcg aatcgcaaag gaacccgata gcgctcgcgc ggcaatcagg
     9361 gccgtcatga aagcccataa agcgctcaaa gaagacgtga accgggctac agaagtgggc
     9421 aggaagcgct ttccaccttc ggaagcggag ctgatcgccg aactgatccg gcgtgatctc
     9481 ccctactacg atccaaccat tactgaagaa accgttaccc acatgaaccg ctttgctcag
     9541 gacatcggtt tgctctccgc gccggttgcc tacgatcaag tcgtcgcgac ccagttcact
     9601 catctttgga aggagccggt ttgaacgcgg cagctcaaag tgatgttatc cagtaacgcg
     9661 gcttaacttc gcgctccgag ataaatcatc cgaaaaacct cgccgttgcg ctcaaccagt
     9721 gtctccaacc cgaaaaaacc cgtctattcg ctagcctatc ccaacgggaa cttttttcta
     9781 aagcggcagt ctaatacaat ttaagcccat gttcgccgaa gggataggtg acgccgaatg
     9841 cgtcaggcgc gtacaacaag gtgacaccga ttccttcgaa gttttggtgc ggcgacacga
     9901 gaaggcgata ttcaacttga tttaccg
//
LOCUS       DASSWZ010000040         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_694599, whole genome shotgun sequence.
ACCESSION   DASSWZ010000040 DASSWZ010000000
VERSION     DASSWZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694599"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFN58_00920"
     CDS             <1..333
                     /locus_tag="VFN58_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916578.1"
                     /translation="YPDLRIVIRSSGGGLPLLLHRFFWKHKGPRGEQRYSEIFLEHFL
                     IDCASSDARTLSCLIDPLGEEHVVFGSDYCGGLGPLKKALASVENQTNPEYLKSFTEK
                     NSRRLLHI"
     gene            369..1601
                     /gene="lhgO"
                     /locus_tag="VFN58_00925"
     CDS             369..1601
                     /gene="lhgO"
                     /locus_tag="VFN58_00925"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HET8916579.1"
                     /translation="MASQYDVTIIGGGIVGLATALKILDAYPRTKLLILEKEAQLARH
                     QTGNNSGVIHSGLYYRPGSLKARSCVTGRKALIEFCNENSVPYELCGKVVVATSQEEL
                     PPLDELHRRGVANGLQGLEIIGTERLKELEPHAIGIKGLRVPETGIIDYKKVAEAYAA
                     RVRSAGGDIRLSQKVLGIIEARQEIVLQTSGGDYRTNYLINCCGLHSDIVAKMMKTLN
                     AAGNSDEHRIIPFRGEYYKIAPERQFLVHNLIYPVPDPTFPFLGVHFTRMAKGGVEAG
                     PNAVLALAREGYHHTDINLGDLWQTASFKGFWAMAGKYWETGFGELYRSLNKPAFVRA
                     LQKLVPEIRESDLVPGGAGVRAQAVSASGALVDDFVIKESRNAIHVLNAPSPGATASL
                     AIGQAITDMAAKNFELKN"
     gene            1626..2153
                     /locus_tag="VFN58_00930"
     CDS             1626..2153
                     /locus_tag="VFN58_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET8916580.1"
                     /translation="MIIEYKGKRPNISPSAFIAPTAVLIGDVTVGEEASVWWGAVLRS
                     DQNANPIVVGDRTSVQDNCVIHSGEAPTIIEADCTIGHGAIMEGPLVKRGAIIGMNVT
                     LLENVEIGEEALIAAGSVVVPHTKIPPRVLAAGSPAKVKKELTGEALHWIKMGSSTYV
                     NLCRSYLAGDYKIIA"
     gene            2278..3018
                     /locus_tag="VFN58_00935"
     CDS             2278..3018
                     /locus_tag="VFN58_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET8916581.1"
                     /translation="MLHAIKKTRIHEEVVTQIHDLIREGRLKAGDQLPSERELSETFK
                     VSRTSVREALRALETKGLIISRTGMGNFIADLPIESLIAPLAKLLIEEKTALADIFEL
                     RKLIEPHIAALAAERATKKDIERMKSILEKQSEAVSRGETGVDADAELHFAIGQATQN
                     QALEKLVSGLMEILSHSREESLQTLDRRKASIESHRKILAAIEQHNRTKAQEAMFHHI
                     EQVEENVLSSRKPQANHNNNRLEERSVS"
BASE COUNT          768 a          813 c          810 g          643 t
ORIGIN      
        1 tatccggatt tgcgaattgt cattcgcagt tccggcggcg gccttccctt gcttctgcat
       61 cgcttttttt ggaaacataa gggacccaga ggtgagcagc gctattcaga aatttttctc
      121 gagcacttct tgatcgactg tgcgagctcc gatgcccgca ccctgtcgtg tttgatcgac
      181 cccctggggg aagagcacgt ggttttcggg tctgattatt gcggcggcct gggcccattg
      241 aaaaaggctt tggcatccgt agaaaaccaa actaatccgg aatatttgaa gagcttcact
      301 gaaaagaatt cccgccggct gcttcacatt tagagagcgg ctatttatga cctcgctgcg
      361 ttgaagctat ggcttctcaa tacgacgtca cgatcatcgg cggcggcatc gtcggtttag
      421 caacggctct aaaaatcctc gatgcttatc cccgcacgaa gcttttgatc ctcgaaaagg
      481 aagcccaact ggcccggcac caaaccggca acaacagcgg tgtcattcat tccgggctct
      541 actatcgccc tggatctctg aaggcccgga gctgtgtcac cgggcgaaaa gcgctgatcg
      601 aattctgcaa cgaaaattct gtgccctatg aattgtgcgg taaagtggtc gtggcaacga
      661 gccaggaaga gttgccgccg ctcgatgagc ttcatcgtag aggcgtggcc aacgggctgc
      721 aaggcttgga aataatcgga actgagcggc tcaaagagct cgaaccccat gccattggaa
      781 tcaagggact ccgcgtccct gaaaccggca ttatcgacta caagaaagtc gccgaggctt
      841 acgccgcccg ggtgcggtcg gcgggaggtg atattcgcct gtcgcaaaag gttctcggga
      901 ttatcgaagc ccgtcaagaa atcgttttgc aaacctcggg gggcgactac cgcacgaact
      961 atctgatcaa ttgttgcggc ctccactccg atatcgttgc caagatgatg aaaactctta
     1021 acgccgctgg aaacagcgac gagcaccgaa tcattccgtt tcgtggcgag tattataaaa
     1081 tcgcacccga aagacaattt ttggtacaca atttgattta cccggttccg gacccaacct
     1141 tcccattctt aggagttcac ttcacccgca tggcgaaggg cggagtcgaa gccggaccca
     1201 acgcagttct tgctctcgcc cgcgagggct accatcatac cgatatcaat ctcggagatc
     1261 tgtggcagac cgcttctttc aagggcttct gggcgatggc ggggaaatat tgggaaaccg
     1321 gattcggcga actttatcgc tccctcaaca agccggcttt tgtccgcgcg ctccaaaagc
     1381 ttgtgccgga aattcgcgaa agcgatcttg ttccgggcgg cgccggcgtg cgcgcccagg
     1441 ccgtgtcagc cagtggcgcg ttggtggacg attttgttat caaggaaagc cgcaacgcca
     1501 ttcacgtcct caacgcccca tcccccggcg caaccgcctc cttggccatc ggccaagcga
     1561 ttacggacat ggcggcaaag aattttgaat tgaaaaactg atcgctcagg gaggtcaatg
     1621 ctgtaatgat tattgagtac aaaggcaaaa gaccgaacat ctccccaagc gcttttattg
     1681 cccccaccgc tgttttgatc ggcgacgtca cggtcggcga agaagcgagt gtctggtggg
     1741 gagcggtctt gagatcggat caaaacgcca atccgatcgt ggtgggtgac cgcacgagcg
     1801 tacaagacaa ttgtgtcatt cattctggcg aagctccgac cattatcgaa gcagattgca
     1861 ccatcggcca cggcgccatc atggaaggtc ccctcgttaa acggggcgct attattggaa
     1921 tgaatgtcac tctcctggaa aacgttgaga tcggcgaaga agccctcatc gcggccggca
     1981 gcgtcgtcgt tccgcatacc aaaattccgc cacgcgttct tgccgcaggc agcccagcca
     2041 aagtgaaaaa agagctcacc ggcgaggcct tacattggat caagatgggc tcttcgacat
     2101 acgtgaattt gtgccggagt taccttgcag gcgactacaa gatcatcgcc tagggaagac
     2161 ggcggcaccg attcacggga tctctcaaca ggcgggcggc gtttagaggg gtacccagac
     2221 ccacttgaca aatagtgaca aaatggatat tggtcagacc agcagacagc tagacctatg
     2281 ctccacgcca ttaagaagac ccggattcac gaggaagtgg tgacccagat tcatgatctc
     2341 atcagagagg gcagactcaa ggcgggcgat cagctgccct ccgagagaga actttcagag
     2401 acatttaagg tgagccggac ctccgtgcgt gaagctctcc gggcattgga aactaaagga
     2461 cttattatca gccggacggg aatgggaaat tttatcgccg acttgcctat tgaatcttta
     2521 atcgccccct tggctaaatt gctcatcgag gaaaaaactg ccctcgccga catttttgag
     2581 ctccgtaagc tcattgaacc gcacatcgcg gcactggctg ccgagcgcgc aaccaagaaa
     2641 gacatcgaga gaatgaaaag cattctggaa aagcagagcg aagcagtgag tcgaggggag
     2701 accggagtag acgccgatgc tgagctccat tttgctatcg gacaggcaac tcaaaaccag
     2761 gccctagaga aacttgtctc tggattgatg gaaatattaa gtcacagccg cgaggagtcc
     2821 ctccaaacac tcgatcgcag gaaggcttct atagagtccc accgaaaaat tctcgctgcc
     2881 attgaacagc acaacaggac aaaagcccaa gaagcgatgt tccaccacat cgagcaggtc
     2941 gaagaaaatg tgctttcatc gaggaaacca caggccaatc acaacaataa ccgtttagaa
     3001 gagaggagtg tttcatgatc aagcgcgtag aggt
//
LOCUS       DASSWZ010000041         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_241904, whole genome shotgun sequence.
ACCESSION   DASSWZ010000041 DASSWZ010000000
VERSION     DASSWZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241904"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFN58_00940"
     CDS             complement(<1..241)
                     /locus_tag="VFN58_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004598144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HET8916582.1"
                     /translation="MTYKPQDSYYKKAKQEGYRSRAAYKLLELQQRFRLLRPGDVVVD
                     LGAAPGGWLQVAAKFVGESGKVVGIDLQPIEALREP"
     gene            complement(478..1251)
                     /locus_tag="VFN58_00945"
     CDS             complement(478..1251)
                     /locus_tag="VFN58_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8916583.1"
                     /translation="MKSLRLPVLVMILCLALGPKVSIAAPDEYDDSQSNPFRVAAYLL
                     YPAGFILEWTVFRPFHFLVSSTAPTEAFFGHVPHPPVISDPRPYPYYGVSPRVPLTEV
                     PPPAKVTAAPAPPKEVIKIVEVPVEKIVVKEVVKEVPKIVEVERVVFPNVAFRFDSAE
                     LTELGKGEVYLAAQRLKEKANVTIAIEGHTDDVGNSEYNDKLGMRRAETVMKELASLG
                     VDRGRMSAASLGETKPLIGQETAWARAVNRRVEFQVKGQ"
     gene            1473..2234
                     /locus_tag="VFN58_00950"
     CDS             1473..2234
                     /locus_tag="VFN58_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8916584.1"
                     /translation="MAVKVESKERVVRITLERPPLNVLDIPLLRELDAVLTSCAHEAE
                     TAVVVIEGAGQRAFSAGVDVRDHTRANVPEMLDAVHGVIRKLFLVPQVTIALVQGVCL
                     GGGCELATSCDFIIASEDSSFATPEIHVGCYPPVALARFSSLIGYRRAAEMILTGRKF
                     SAQEALTIGLINRALPSDQLEAGLAALLEELLGKSGAVLRIAVKGLRELSLKGFSDAL
                     RRSEDLYRNELLHTEDVEEGIRAFLEKRKAKWMHR"
     gene            2304..3149
                     /locus_tag="VFN58_00955"
     CDS             2304..3149
                     /locus_tag="VFN58_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8916585.1"
                     /translation="MRWIKLIAILSLGLCFFGVLVVLHLCISLLRLPNRWKIISRWIR
                     GFTWSMRRILNIKITVEGDTGQLERGGYVIISNHFGYLDGIVLGSLFPVVFVSKSEVK
                     SWPVVGQWTTLCGTVFINRQRKDLIPLAVKEISEKLEQQANILLFPEGAATNGERMLP
                     FQTAPLAAPLRSRAIIVPITLAYQSVEGQPVSAANRDLIYCYDDMPDVPHFWKLLALH
                     RVEARVTIQPRVDCSRYEDNSAGRKKLAEDCYNRILGRARKQDCSHKEGETATPGNSR
                     GLFSS"
     gene            complement(3135..3587)
                     /locus_tag="VFN58_00960"
     CDS             complement(3135..3587)
                     /locus_tag="VFN58_00960"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET8916586.1"
                     /translation="MKFTQSAQIPVAREPLWNFLMDVPKVAQSLPGVESVSKIDDTTY
                     QGALKVRVGPISLNLQGKIIVEEQDHENWRAALRAEAKDRMAAGAVNGKTTMMLKELG
                     ADQTELLVETDVSILGKIGEFGQPIIRKKADAMLKEFVENIKKQLTGK"
     gene            complement(3584..4516)
                     /locus_tag="VFN58_00965"
     CDS             complement(3584..4516)
                     /locus_tag="VFN58_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8916587.1"
                     /translation="MIPFEYRTPKNLKEVHATLKEFGTDAKLIAGGTALVIMMKQRLV
                     RPSCLVSLRSVRGLNAIELKDGGLRIGGLATHREVEDSSLVRRRLPLLAETYHHVATI
                     RVRNRATVGGGLAHADPNQDPPPTLIALGATVKATSPNGSRVIPLDEFFTDYYETSLK
                     PDEIITELFVPRLAANAGGAYLKFLPRTADDYATVSAAAVLTLDKSKKTISDVRIALG
                     SVGTTPIRATAAEAVLRGQPVKAEAFAEAAEKAKEAVDPVSDFRGSAGYKKEMAGVFV
                     RRALEKALAGIRQLARAKPAPKTTKKTVKKRRKR"
     gene            complement(4528..>4827)
                     /locus_tag="VFN58_00970"
     CDS             complement(4528..>4827)
                     /locus_tag="VFN58_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET8916588.1"
                     /translation="MVSSCLELAVFADGHDLTTVEGLAKDGELHPVQKSFIQYGGFQC
                     GICTPGQVVAAKALLDENPRPNEEEIKDWMMGNLCRCTGYYKIIESIQKAYQFAK"
BASE COUNT         1039 a         1292 c         1325 g         1171 t
ORIGIN      
        1 taggttcgcg caaggcttcg atgggctgga gatcgatgcc aacaactttt ccactctcgc
       61 cgacgaattt tgcagccacc tgaagccagc cacctggagc ggcccccagg tcgacaacaa
      121 catcaccagg tctcaggaga cgaaaacgct gttgtagctc aagcagctta tacgcggctc
      181 gcgatcggta gccttcttgc ttggcttttt tgtaataact gtcctggggc ttataggtca
      241 tcgactttta ctgatcctag cacattgctg caaaaactct gctcacaggc tgctcaaaaa
      301 gacctcaaag gcgaggcgcg cgaagatcga tgagcggagg cgtactcttt ttgtacgttg
      361 acgcgaaatc ggtcgagcga aacgaagcat atgagtcttt ttcagtagcc tgcgacgaga
      421 aaaaaaagcc cagcctaaga gttaggctgg gcctcgaccg tcaaatctta aattatctta
      481 ctgaccctta acttgaaact caacccggcg attcacggcc cgcgcccagg cagtttcctg
      541 gccgatcaac ggtttagttt ctcccaggct ggcggcagac attcttcccc ggtccacgcc
      601 caaggacgcg agttctttca tgactgtttc ggcacggcgc attcccaact tatcattgta
      661 ctcactgttt ccgacgtcat ctgtatgacc ctcgatcgca atcgtaacat tagctttctc
      721 tttaagcctt tgcgcggcaa gatagacttc ccctttgcca agttccgtga gctccgctga
      781 gtcgaaacgg aaagctacat tagggaagac gacccgctcg acctcgacaa tcttgggcac
      841 ttctttgacg acttccttaa cgacaatttt ttcgacggga acctcgacga tcttaatcac
      901 ctccttcggt ggcgctggtg ccgccgtcac ttttgccgga ggtggcacct ccgtcaatgg
      961 gactctaggg gatactccat aatatgggta gggtcgaggg tcggaaataa cgggagggtg
     1021 aggcacatgg ccgaagaagg cctcagtggg cgcagtgctg ctaacaagaa aatggaaggg
     1081 tctaaaaacc gtccactcta gaatgaatcc agcaggatag agcaagtaag ccgcaactct
     1141 aaaagggtta gattggctat cgtcgtactc gtcaggcgca gcgatgctaa cttttggccc
     1201 aagggcgaga catagaatca tgacgagcac tggtagtcgt agacttttca taatagcctc
     1261 cttatcactt gcaagcaccc aagtttaagg tctggccaca cgcttgtcaa actcgtgggg
     1321 gcagccattc ccttgtaaaa cggggctcgt ttatgcgaga aaaagcctca gaccctcgct
     1381 cgggatttcg cctggaagtc gagggttcgg aaccggtccc acgggcttgg tttagcgcag
     1441 aaagaaacaa caggtttcgg agataccgaa cgttggctgt aaaggttgaa agcaaggaac
     1501 gggtggttcg catcaccctc gaacgtccgc cgttgaatgt tctggacatc ccgttgttgc
     1561 gggaacttga tgcagtgctg acgtcctgcg cgcacgaggc tgaaaccgcc gtcgtggtca
     1621 tcgaaggggc tggccagcgg gcgttctccg caggcgtcga tgtccgcgat cacacgcgcg
     1681 caaatgttcc cgagatgctc gacgccgtgc acggtgtgat ccgcaaactt ttcctggtcc
     1741 ctcaagtcac tattgccctg gtccagggtg tctgtcttgg cgggggttgc gaattggcaa
     1801 cttcgtgcga cttcattatc gcctcggaag atagttcttt tgcgacgcct gagatccatg
     1861 ttggatgtta tccgccagtc gctctggcgc gtttttcctc gctgatcggt tatcgtcgtg
     1921 ccgcggagat gattcttacc ggacggaaat tttccgctca ggaagcctta accatcggat
     1981 tgatcaaccg tgcgctgccc agcgatcaac tcgaggccgg cctggcagct ttgttagaag
     2041 aactgcttgg caagagcggg gcggttttac gtatcgctgt gaagggtcta cgagaacttt
     2101 ctcttaaggg attttccgat gcgctcagac gatcagagga cttgtatcgc aacgagcttc
     2161 tccatacaga agatgtcgaa gagggaattc gcgcattcct cgagaaaaga aaagccaaat
     2221 ggatgcaccg ttaaaatatt gctcaatgct aaaatggaaa gattataagt gccgtttcgg
     2281 tctgatccag tcccgggtgt gccatgcggt ggattaagct gatcgctatt ctttccctgg
     2341 ggctatgctt cttcggcgtc ctagtcgtcc tgcacttgtg catcagcctg ctaagacttc
     2401 cgaatcgctg gaagattatc agtcgctgga tacgaggctt tacctggtca atgagaagga
     2461 ttctgaatat caagataacg gtggagggcg acacgggaca gctcgaacgc ggcggttatg
     2521 tcatcatcag caaccatttc ggctatctcg atgggattgt tcttggaagc cttttcccag
     2581 tggtttttgt ctccaagagc gaagtcaaaa gctggccggt ggttggtcag tggacaacgc
     2641 tttgcggcac ggtttttatc aatcggcaaa gaaaagacct cattccgctg gcggttaagg
     2701 agatcagcga gaaactagag caacaagcaa atattttatt gtttcccgag ggggctgcga
     2761 ccaatggtga gcgcatgctc ccctttcaga ccgctccgtt ggcggcgcct ttgaggagcc
     2821 gggcgattat cgtaccgatt actttggctt atcaaagtgt cgaggggcag cccgtgtcgg
     2881 cggccaaccg tgacttgatt tattgttacg acgatatgcc cgatgttccc cacttttgga
     2941 agcttctcgc gctgcaccgg gtcgaagcca gagtgacgat ccagccgaga gtcgattgtt
     3001 cccgatatga agacaactcg gcaggccgga agaagcttgc tgaggattgt tacaaccgta
     3061 ttttggggcg agcgcgcaaa caagattgct cgcacaaaga gggggaaacg gcaacgccgg
     3121 gaaactcgcg ggggctattt tccagttaac tgttttttga tgttctcgac gaactctttg
     3181 agcatggcgt cggctttctt gcggatgatc ggttggccaa actccccaat cttgcccaaa
     3241 atactcacgt ccgtctccac caggagttcc gtctgatcgg cgcccaactc cttgagcatc
     3301 atagtggttt taccgttcac agcacccgcg gccatgcgat ctttcgcctc ggcacgcagg
     3361 gccgcgcgcc agttctcgtg gtcttgttct tcgacgatta tcttgccctg aagattgagg
     3421 gatatcggtc cgactctgac tttcagtgct ccctgatagg ttgtatcgtc gattttgctg
     3481 acagactcaa ctcccggcag cgactgcgct accttgggaa cgtccatgag aaagttccaa
     3541 agaggttcgc gggcaactgg aatctgcgcg ctctgggtaa atttcaacgc tttctccttt
     3601 tcttgaccgt cttcttggtt gtctttggcg ccggtttggc tctggctaac tggcgaatgc
     3661 cggcgagggc cttttcgagg gcgcgccgga caaatacgcc ggccatctct ttcttgtagc
     3721 ctgccgagcc gcgaaagtcg ctgactggat cgactgcttc cttggccttc tccgcggctt
     3781 cagcgaaagc ttcggctttg acgggctgac ctcgcagaac ggcttctgcg gccgtggccc
     3841 gaatcggtgt ggttccgacg cttccaagag cgatccggac gtcactgatg gttttcttgc
     3901 ttttatccaa ggtgagcacc gccgcagccg acaccgtggc ataatcatcg gccgtccggg
     3961 gtaagaactt caggtaggcc ccgccggcat tggctgctag acggggaaca aacaattcgg
     4021 tgataatctc gtcgggtttg agcgaggttt cgtagtagtc cgtgaaaaac tcatcgagcg
     4081 gaatgacccg gctcccgttc ggtgaagtcg ctttcaccgt cgctccaagc gcaatcaagg
     4141 ttggcggcgg gtcctggttc ggatcggcat gcgccaagcc tccgccgacg gttgccctat
     4201 tgcgcacccg aatcgtggcg acgtggtgat aggtctcggc gagcaagggg agtcgccggc
     4261 gcaccagtga cgaatcttct acttcacgat gcgtggctag accgccgatg cgcagcccgc
     4321 cgtcctttag ctcaatagca ttgagcccgc gtacggagcg aaggctgacg agacagctcg
     4381 gtcggactag acgctgcttc atcataatca ctaaagcggt cccgccggcg ataagcttcg
     4441 cgtcggtccc gaattctttc agcgtggcat gaacttcctt gagatttttg ggcgtacggt
     4501 attcgaaagg tatcatggct gtgtccctta tttcgcgaat tgatatgcct tctggatcga
     4561 ttcgataatc ttgtagtagc ccgtgcagcg gcagaggttg cccatcatcc aatctttgat
     4621 ctcttcttcg ttaggccggg ggttctcatc gaggagtgct ttggcggcaa ccacctgacc
     4681 cggagtgcat atgccgcact gaaagccgcc atactgaata aagctcttct gcaccggatg
     4741 gagctcgccg tctttggcca agccttcaac ggtggttaag tcatgaccat cggcaaaaac
     4801 cgccaactcc aaacaggagc tcaccat
//
LOCUS       DASSWZ010000042         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_157046, whole genome shotgun sequence.
ACCESSION   DASSWZ010000042 DASSWZ010000000
VERSION     DASSWZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157046"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFN58_00975"
     CDS             <1..726
                     /locus_tag="VFN58_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916589.1"
                     /translation="NDGPIAAVEQGMDIVMIAASSKITHMLIGGKNYRTYEDLRGATI
                     GASTLTSGTAFVLRRALKTKGLEYPRDYKLLNVGGTASSFAALSAGQIAAAMQAVPNA
                     FQAQEAGFNVIGRIADIFPTYLLSAYSVRRSWAEKNRPQVVRFLKGVLQAKKWFEQDK
                     KGAVEYLAKEFQLTPSLAERGLDYYLTYQAWPPELEIDMEGLKTVVDIYAEQTGMKGP
                     LPNPEKYVDMSYLKQAWKELGWK"
     gene            749..1555
                     /locus_tag="VFN58_00980"
     CDS             749..1555
                     /locus_tag="VFN58_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916590.1"
                     /translation="MITVEGEQIEIIDAHSHMGARKKLAIHQIPPIMKFMAEDMLESM
                     DNAGVDGVVTFAIGIGEPSDYRETNQYISNAMKQHAGRIHGFMRLSPGNGPKDTLQVL
                     DEGVKLGLKGIKIHPLIEHCPANDKEKVYPLMEAAQHYGLPVLFHCGLGEDASPKRIG
                     EVAKDFPKLPIIMGHSGLVEGVRDVVEIAKKCENVHMDSSGVGWLPFFCESISWAGPD
                     RVLYGSDTPFNPMEWEIEKIVKHAQRHLKLKIEDLRLIMSGNIKRLLKLN"
     gene            2108..>3102
                     /locus_tag="VFN58_00985"
     CDS             2108..>3102
                     /locus_tag="VFN58_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET8916591.1"
                     /translation="MKIISISIGGSHGINVAETKDDFSCTDFPPVNPYKEGAFIVSMP
                     MQSFGEPHRAPLRKSEKVWPPGGFFFDDEKFFLKESTVLAPCHGVLEVQRPHVGGKFL
                     FVGNDKLWIRGVTYGAFRPGPNGQEYHDTKVLERDFSLMAANGLNAVRIPHTTPPRGL
                     LDIAQRHGLRVMVGLSAEQYAGYLIDRKGAPDIEKLIRSKARICAGHPALLCYALGNE
                     IAAPVVRWLGRERVESYLERLYWAVKAEDPNALVTYVNYPTTEYLHLPFLDLVTFNVY
                     LESQDQFDGYLARLHNIAGDRPLLMSEIGLDSLRNGEATQAQALEWQLRSTFSPG"
BASE COUNT          776 a          799 c          825 g          702 t
ORIGIN      
        1 aacgacggcc ccatagccgc ggtagaacag gggatggata tcgtaatgat cgccgccagc
       61 agcaagatca cacacatgct gatcggcggg aaaaattata gaacctacga ggacctgcgc
      121 ggcgcaacca tcggcgcttc aaccctcact tcagggaccg catttgttct cagacgggcg
      181 ctaaagacca aaggcctcga gtatccgcgc gactataaac ttctgaacgt tggcggcacc
      241 gcttcttctt tcgcggcctt gagcgccggc cagattgcag cggccatgca agcggttccc
      301 aacgcttttc aggctcaaga agcgggattc aatgtgatcg ggcgcatcgc ggatattttc
      361 cccacctatc tgctttcggc ctattcggta aggcgcagtt gggcggagaa aaatcggcct
      421 caagtggttc gttttttgaa aggcgttctt caggcgaaga aatggttcga gcaggataag
      481 aagggtgccg tggaatatct tgccaaagaa tttcaactca cacccagcct cgctgagagg
      541 ggactcgatt attatctcac ttaccaggca tggcctccgg agctcgagat tgatatggag
      601 ggattaaaga cggtggtgga tatctatgcg gagcaaacgg gcatgaaggg gccgctcccc
      661 aatccagaaa aatacgtcga tatgagctac ctgaaacaag cctggaagga actgggctgg
      721 aaatgatcac aatgtcgagg aggcaactat gattactgtc gaaggggaac agatcgagat
      781 catcgacgcg cattcgcaca tgggcgcgag aaaaaagtta gctatacacc agattccccc
      841 gattatgaaa ttcatggcgg aggacatgct ggagtcaatg gacaatgccg gagtggacgg
      901 agtcgttact ttcgccatcg gtatcggcga gccgtcggat tatcgcgaaa ccaatcaata
      961 catttccaac gccatgaaac agcatgccgg gcgcattcac ggtttcatga ggctgagtcc
     1021 cggcaacgga ccgaaggata cgcttcaggt gctggacgaa ggggtcaagc tcggactcaa
     1081 agggatcaag attcatccgc tgatcgaaca ttgtccggcc aacgacaaag agaaagtcta
     1141 cccgctgatg gaggcggcgc agcactatgg gttgcccgtt ctctttcact gcggcctagg
     1201 cgaagacgcc agtccgaaac ggattggcga ggtggccaaa gactttccca agctgcccat
     1261 catcatgggt cattcaggtt tggtggaggg agtccgagac gtcgtcgaga tcgccaagaa
     1321 atgtgaaaac gttcacatgg acagctcggg cgtgggatgg ctgccgtttt tctgcgagtc
     1381 gatctcctgg gctggacccg atcgagttct ctacggttcg gacacgccgt tcaatccgat
     1441 ggaatgggaa atcgagaaaa tcgtcaagca cgcgcagcgg cacttgaagc tcaagatcga
     1501 agatctccgc ctgatcatgt ccggcaacat caaacggctg ctgaagctaa actagatcga
     1561 aacaaaacga tccattgccg gttagcgggc agctgccttg tcctagcggg cttgtaatgg
     1621 atccgtgccc gagagttttt tgaacagttc tatagtctcc ttgtcacgcg gcactcccga
     1681 ataattttct cttctcttcc gcgtctgacc cagcgaaatc ttcaagcttt gggcagacga
     1741 ctcgagcacc cgcagcgcca ctgacgctta agatcgaaga tcttcgccct gattatgtcg
     1801 ggcaacattc aagcaaactc cacggcgaga tcataataga tgtggtcgtt caccacgcaa
     1861 tccgatccta cccgactcga acatcccgca agactagatt tgcgcgtcat aattggccga
     1921 tataacagcc ccgatttcaa cgatcagact gaagacagca aaagaaacgg agctaggcat
     1981 cgattgttct gtgaggttag ttctcattta tccctacatc agaaacggtt tttctaaaat
     2041 gatgtaggaa ctatcctgca cttactcgtc gccgtgtctt tcgacagcga gcaaacccgt
     2101 gaaatcaatg aaaataatct ctatttcaat aggcgggtca catggcatca atgttgcgga
     2161 aacaaaagac gatttctcat gtacagattt tccaccagtc aatccctata aagaaggagc
     2221 gttcatcgtg agtatgccga tgcagtcatt cggcgagccg catcgcgcgc ctttaagaaa
     2281 atcggagaaa gtttggcctc ctggagggtt tttcttcgat gacgagaagt tttttttgaa
     2341 agaatccacc gttttggccc cgtgccatgg ggttcttgaa gttcaacgac cacatgtagg
     2401 cggtaaattc ttatttgttg gcaacgataa actctggatt cgtggtgtca cctacggagc
     2461 attcagaccg ggtccaaacg gtcaggagta ccatgacact aaagttctgg agagggactt
     2521 ttctctgatg gccgccaacg gactgaatgc cgtccgtatt ccacatacga cgccaccacg
     2581 ggggcttctc gacatagctc aaaggcacgg ccttcgggtt atggtcgggc tctctgccga
     2641 acaatacgca ggttatctta tcgatcggaa aggggccccc gatatcgaaa agttgatccg
     2701 gagtaaggcg cgtatctgcg ccgggcatcc cgctctgctt tgctatgccc tcggcaatga
     2761 gattgcagca cctgttgttc ggtggctggg acgcgagcga gttgagagct atctcgagcg
     2821 cttgtactgg gcggtgaaag cagaagaccc gaacgccctc gtcacttatg tgaattatcc
     2881 cacaacggaa taccttcatc ttcctttcct ggaccttgtg actttcaacg tttaccttga
     2941 atcgcaggat cagtttgacg ggtacttggc ccggcttcat aatattgccg gtgatcgacc
     3001 tctactcatg agcgagatcg gcctcgatag tcttcgaaac ggagaggcaa ctcaggcgca
     3061 agcgctcgag tggcaactcc ggagcacctt ctcacccggt tg
//
LOCUS       DASSWZ010000043         7700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_468428, whole genome shotgun sequence.
ACCESSION   DASSWZ010000043 DASSWZ010000000
VERSION     DASSWZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468428"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..929
                     /locus_tag="VFN58_00990"
     CDS             144..929
                     /locus_tag="VFN58_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916592.1"
                     /translation="MALLELRGISKRFGGLQALNRVDLTLEDGMIAALIGPNGAGKTT
                     LFHTLTGIYRPDEGEIRFSGRSLVGLKPDKITGAGISRTFQNIRLFARMTVLENILVG
                     MHCRIRTSLWGTLLQSQGFHRQESDAARRAMELVELAGLRGKENEWAHQLSYGEQRRL
                     EIARALAASPKVLLLDEPTAGMNTTEAQSLMLLFKDLIGRYVQAVLLIEHNMRVVMGI
                     SHRVHVLDYGEKIAEGDPDEVRRDPRVIEAYLGRGSWVGDAQS"
     gene            916..1617
                     /locus_tag="VFN58_00995"
     CDS             916..1617
                     /locus_tag="VFN58_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916593.1"
                     /translation="MLKVKDVHVYYGAIHALKGVSFFIEKGELVTLLGANGAGKTTTL
                     KTLSGLLRLHSGRIELEGASLNGADPHVIVQRGVGHAPEGRKIFPRFTVMENLHIGGY
                     TRSKHSLGPDIERVFELFPRLKERQKQVAGTLSGGEQQMLAIGRALMVRPKLLLLDEP
                     SMGLAPKIVEQILETIASINKAGVTVLLVEQNAAMALAISHRGYVLETGEVVLQGSAS
                     ELAGNDFVRQAYLGG"
     gene            1684..2391
                     /locus_tag="VFN58_01000"
     CDS             1684..2391
                     /locus_tag="VFN58_01000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET8916594.1"
                     /translation="MQYQTRPMMFGGGTVTPAVKFLLIVNTAVFFLQLFLPSEILLLF
                     GLVPSMIWHDFYFWQLFTYQFLHGGLFHLLFNMLALWMFGCDLERRWGSNFFLKYYFV
                     SVVGGGILNALLVPGQIVPSIGASAGIYGILLAYGLIYPNRIVYFYFLFPIKMKYFVW
                     IIGGIAFYSSMTAGQSGIAHLAHLGGMVFGYLYLRGGNPWERFRLYLDQRRLARLKRR
                     FQVIQGRRDDDSKPTLH"
     gene            2543..3856
                     /gene="mgtE"
                     /locus_tag="VFN58_01005"
     CDS             2543..3856
                     /gene="mgtE"
                     /locus_tag="VFN58_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET8916595.1"
                     /translation="MADKNFTPAELYEAWPVLSMEERVEGFEFLQREEADDFFLQLSA
                     RDRAQLVLALPAGERRLWIRLLAPDDAADLIQEAPLEERENLLALLDDSTRREVKGLL
                     DYAEDEAGGLMNTRYSRLRADMTVDEAIAYLRRDARQREKTVYYVYVVDGEEHLLGVV
                     SFRDLIVAPGDKHVSDVMRTEVISAPDDLDQEALSQLFMRHHLLMMPIVDAEGRIKGV
                     VNVNDIVQVVQEEATEDIQKIGGVATLEGPYLQIPLLLMIRKRAGWLAALFLGEMLTA
                     TAMGRFEAEIARAVVLALFVPLIISSGGNSGSQATTLVIRAMALGELRIRDWWRVIRR
                     ELVTGLGLGLILASIGLVRILAWQGLFNAYGEHYFLVALTVALSLVGVVLWGSIAGSI
                     LPFILRALGFDPASASAPFVATLVDVTGLIIYFTIADVVLRGTLL"
     gene            3940..5337
                     /locus_tag="VFN58_01010"
     CDS             3940..5337
                     /locus_tag="VFN58_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8916596.1"
                     /translation="MKSALQEKIVGKLANIVPPEHITTDENRLDELSWDALSEGRLHP
                     AKRPEVTIPFCSVLPGSTDEVRRVVLLANEERVPIIPYGGGSGLMGGALSVQAGIVVD
                     LRRMDQILDVDTQARSARVQAGTVLEALEKRLNRDNFMLGHDPWTLPVATVGGAISTN
                     SMGYRGGLYGSMGEQVLGLEAVLPDGEVLRTRAVPKSSAGIDLKFLLVGGEGCFGIIT
                     EATIRIFPAPEKRVLHALRFESFEVGYNAIQEMFRLRVQPAVLDFGDADAKFQGGAML
                     YLAFEGIREIVEAEEKRALLVCEKFGAKGLPREEAERFWSDRHEIARRFMQNRRQRRE
                     RSRDGIYRDWIHVALSASKVLPFRAAAMEIAKKHGVQVLESGLWIQPELFSMPLGIHD
                     DGTNKAQLTLEETVDELLHLVHEMGGSMEYTHGVGVKLAPLMAEEHGYGVEVMRRIKK
                     TLDPKGILNPGKMGL"
     gene            5564..6298
                     /locus_tag="VFN58_01015"
     CDS             5564..6298
                     /locus_tag="VFN58_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET8916597.1"
                     /translation="MKLREQIEILATLQNVDREIREKNGIKQELLGELQVKDKEILSK
                     KQEIALLAAACAEKEKDRQGKERVLQDEGKKAMDKRMRMNRIKNIKELQALQREIDQI
                     KQSNGDLEEELIRVLEEIDRIKAELKAKEEEAAMMEEEWQQRQEEMQSKLSGIDQLVS
                     EAYTLRKTIASQIAGDLIARYELIFARRGGSAVVEVAGGICRGCYMNIPPQLWNEIIK
                     SEKVNLCPSCQRILYYKPSAEQEKEA"
     gene            6445..6879
                     /locus_tag="VFN58_01020"
     CDS             6445..6879
                     /locus_tag="VFN58_01020"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HET8916598.1"
                     /translation="MSELDNEWLVMVDGAARGNPGHAGCGAVILDGNGTVVKELSRYL
                     GHATNNVAEYEALLMGLEALFQLGKRRIRVQSDSQLLVRQLNGEYRVKDEKLKVLFQR
                     AVTLLRQFEVYRILHVPREMNKLADRLANKGIDEAIKRSSLG"
     gene            6878..7237
                     /gene="rnpB"
                     /locus_tag="VFN58_01025"
     ncRNA           6878..7237
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFN58_01025"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            7573..>7700
                     /locus_tag="VFN58_01030"
     CDS             7573..>7700
                     /locus_tag="VFN58_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET8916599.1"
                     /translation="MFRGSFEHTLDGKGRLSIPVKFREVLFGKSDERIVITNFVVDG"
BASE COUNT         1753 a         1856 c         2276 g         1815 t
ORIGIN      
        1 ggcgatttta ctttgagtca gattcccgcc cagtttgagc cggcgaaata tgagcggatg
       61 gtttttggtt tgatcctgat tctcatgatg atgtttcggc cgcagggcat cttgccgcct
      121 cggcagaagc ggggacattg aagatggctt tgctggaatt gcgcggtatc agcaagagat
      181 tcggcggatt gcaggcgctg aaccgagtgg atctgacctt ggaagacggc atgatcgcgg
      241 ctctgatcgg acccaacggc gccggcaaga cgaccctctt tcacacgctc acggggatct
      301 atcggcccga cgaaggagag atccgtttta gtggtagatc gctcgtcggg ctgaagcccg
      361 acaaaattac cggcgcgggt atcagccgaa cgtttcagaa tatccgtctc ttcgcgcgta
      421 tgacggtttt ggaaaatatc ttagtcggca tgcattgccg aatccgcact agcctgtggg
      481 ggactctgct ccagagtcaa ggttttcacc gccaggaaag tgatgccgcg cgcagggcaa
      541 tggagttggt cgagcttgcc ggactacggg gcaaagaaaa tgaatgggca caccaactct
      601 cctacggcga gcagcgtcgc ctcgagatcg cccgcgcctt ggccgctagt ccgaaagtgc
      661 tcctgctgga tgagccgacg gcaggaatga acacgaccga agcgcaatca ctgatgctgc
      721 tattcaagga tctcatcggg cgttacgtcc aggccgtgct gcttatcgag cacaacatgc
      781 gcgtggtgat gggcatctcg caccgcgttc acgtcctcga ctatggggaa aagatcgccg
      841 aaggagaccc cgacgaggtg cgccgagatc cgcgcgtcat agaagcctat ttgggacgtg
      901 ggtcgtgggt gggagatgct caaagttaag gatgtgcacg tatactatgg cgctatccac
      961 gctttgaaag gagtttcttt cttcatcgag aaaggcgagt tggtcacctt gctgggtgcc
     1021 aacggcgcgg gcaagacgac cacgttaaaa accctttcgg gcttgttgcg gctgcacagc
     1081 ggacggatcg aattggaagg cgcttctttg aacggcgccg atccgcatgt gatcgtgcaa
     1141 cggggcgtcg gccacgcgcc agaagggcga aaaatctttc ctcggttcac cgtaatggag
     1201 aatcttcaca tcggcggcta cacgcgctca aaacactctt tgggcccgga catcgaacgc
     1261 gttttcgaac tttttccaag gctcaaggaa cgacagaagc aggttgccgg aactctgtcc
     1321 ggaggcgagc agcagatgct cgccatcggg cgcgcgctta tggtgcggcc gaaattgctg
     1381 ctgctcgacg agccgtccat gggactcgct cccaagatcg tcgagcaaat cttggaaacg
     1441 attgcatcta ttaacaaggc cggcgtcacg gtacttttgg tcgagcaaaa tgccgccatg
     1501 gccttggcta tctcacaccg cggctatgta ttggaaaccg gagaggtggt cctccaaggc
     1561 agcgccagtg agcttgccgg aaacgatttt gtccggcaag cctatctggg cggttgatct
     1621 gggtcatcct caattggggc gagcgctttc aattcagcgc gccgacagct attctgaaat
     1681 tgcatgcagt accaaaccag acccatgatg ttcggcggcg gcacggttac tccggcggtt
     1741 aaattcctgc tgatcgtcaa taccgcagtc ttcttcctgc agctgtttct gccttctgaa
     1801 atcttgttgc tgttcgggct cgtcccctcg atgatctggc acgactttta tttttggcag
     1861 ctcttcacat accagttttt acatggcgga ctgtttcatt tgttgttcaa catgctggct
     1921 ctttggatgt tcggctgtga cctggagcgg cgctggggca gcaatttttt cctcaagtac
     1981 tattttgttt cagtggtcgg cggcgggatc ttgaatgcgc tgcttgtgcc aggacaaatc
     2041 gtgcccagta tcggtgcctc ggccggaatt tacgggattc ttctcgcgta cgggcttatt
     2101 tatcccaatc ggatcgttta cttttacttt ctctttccga tcaagatgaa atacttcgtc
     2161 tggattatcg ggggaatcgc gttttattct tcgatgactg ccggccaaag cggtattgct
     2221 cacctcgccc acctaggagg catggtcttt ggttacctct atctccgcgg cggaaatcct
     2281 tgggaacgct ttaggctgta tctcgatcag cgccgcctcg cccgcctgaa acgccgcttc
     2341 caagtcattc aagggcgacg ggatgatgat tccaagccga cgttgcattg acccaaatcg
     2401 cgtcctggtt tttcccgtgc cgaccgggtt tccttttctt cgccaataat catgaattga
     2461 atgtttctgc gattgcacat gtttgatgat gtgctaggaa tagccccatt aatctctgcg
     2521 gcatgatcgc gggataaaaa acatggcgga caagaacttc actccggctg aactctacga
     2581 agcctggccc gtgctttcta tggaggagag agtagagggt tttgagtttt tgcagcggga
     2641 ggaggcggat gattttttcc tgcaattgag cgctcgcgat cgagcgcaac tggttttggc
     2701 gttgccggcg ggggagcggc ggctatggat cagattgttg gcgccggacg atgccgccga
     2761 cttgattcaa gaggcgccgc tcgaagaacg agagaatctg ctggcgctgc tggatgattc
     2821 tactcgaaga gaagtgaagg ggctcctcga ctacgcggag gatgaagccg gggggctgat
     2881 gaacacgcgc tactcccggc tgcgtgccga catgacggtg gacgaggcga ttgcctacct
     2941 tcgtcgggac gcgcgccagc gggagaaaac ggtctactac gtctatgtcg tggacgggga
     3001 agaacatcta ctgggagtgg tttctttccg cgatctgatc gtggccccag gcgataagca
     3061 cgtcagtgat gtaatgcgta ccgaggttat ttccgctccc gatgatttag accaagaagc
     3121 gctgagtcag cttttcatgc gccatcacct gttgatgatg ccgattgtcg acgcggaggg
     3181 acgaatcaaa ggcgtcgtca acgtgaatga tatcgtccag gtagtccaag aggaagccac
     3241 tgaagacatc cagaagatcg gtggcgtggc gacgttggag ggaccctatc tacagattcc
     3301 tcttttgctg atgattcgaa agcgggcggg ttggctggcg gctctgttct tgggcgagat
     3361 gctcaccgct actgccatgg gtcggttcga ggctgagatc gctcgggcgg tggttttggc
     3421 tctgtttgtg ccgttgatta tcagcagcgg cggtaactct ggatctcagg caaccacgct
     3481 ggtgatccgt gccatggcgc tgggagaact gagaattaga gattggtgga gagtaatccg
     3541 gcgcgagctg gttacgggat tggggttggg gttgattttg gcatccattg gcttggtccg
     3601 gattctcgcg tggcagggtt tgttcaatgc ctatggcgag cattacttcc tcgtcgcttt
     3661 gaccgttgcc ttgagccttg ttggggtggt gttgtggggc tccattgccg gttcgatttt
     3721 gccgtttatc cttcgcgccc tgggcttcga tccggccagt gcctccgccc ctttcgtggc
     3781 aacactcgtg gacgttacag gcttaatcat ctatttcacg atcgccgatg tggttttacg
     3841 cggtacgttg ttgtgacgtg tcggttcccg acgtttaatt ccgtggcttc ttccttgatc
     3901 ggcttttcga tataatgagc ttggcaccgg atgggttcga tgaagagtgc tttacaagaa
     3961 aagatcgttg gcaaactggc caacatcgtt ccgcctgagc atatcaccac tgatgaaaac
     4021 cgccttgacg aattgtcatg ggacgcgctg agcgaggggc gacttcatcc tgcgaagcgg
     4081 cctgaagtga cgatcccctt ttgcagcgtc ttgcctggtt cgaccgatga agtcagacgg
     4141 gttgtccttt tagccaacga agaaagagtg ccgattattc cctatggtgg tggatcggga
     4201 ctcatgggtg gagcgttgtc ggtccaagcg ggaatcgtcg tcgatttgcg acgaatggac
     4261 caaatcctcg acgtcgatac tcaggcgcgt tcggctcgtg tgcaggcagg cacggttttg
     4321 gaagcgctgg agaagcggtt gaaccgagat aatttcatgc ttggccatga cccgtggacg
     4381 ttgcctgttg ccacggttgg cggcgcgatt tccaccaata gcatgggata ccgtggcgga
     4441 ctttacggct ccatgggtga gcaggtcctg ggactagaag ctgtgttgcc tgacggtgaa
     4501 gtgctccgca cccgggcggt tcccaagtcg tccgccggca tcgatctcaa gtttcttctc
     4561 gtcggtggcg agggctgctt tggaattatc accgaagcca cgattcggat ttttcccgcg
     4621 ccggagaagc gcgtgcttca tgcacttcgc ttcgagtcgt ttgaagttgg ctataatgcc
     4681 attcaagaga tgttccgcct gcgcgtacaa ccggccgtcc ttgatttcgg cgatgcggac
     4741 gcgaagtttc aaggcggtgc gatgctctat ctcgcgtttg agggcattcg tgaaattgtc
     4801 gaggccgaag agaaacgcgc gctcttggtc tgtgaaaaat ttggcgccaa gggcttgccc
     4861 cgggaagagg cggaaagatt ctggagcgat cgacacgaga tcgcccgacg gttcatgcaa
     4921 aaccgccggc aacgaaggga acgcagcagg gatggcatct atcgagattg gattcacgtc
     4981 gcgttatccg cctcgaaggt cttgccgttt cgagcggcag caatggagat tgccaagaaa
     5041 catggagtgc aagtcctgga gagcggcctc tggattcaac cggagctttt ttccatgcct
     5101 ttgggtatcc acgacgacgg cacgaacaag gcgcaactca ctttggaaga aacggtcgac
     5161 gaactgctgc atttggtaca cgaaatgggc ggatccatgg aatacactca cggcgtcggc
     5221 gtgaaattag cgccgctgat ggccgaagaa catggttacg gcgtggaagt tatgcgtcgg
     5281 atcaagaaaa cgctggatcc caaggggata ctgaatcccg gaaaaatggg cttatagtcc
     5341 taggggaaag gtggcgggag caacgcgcgc gctaaaggcc gagaatagga gtggtcatga
     5401 gggtcaatac cactttacaa tagtggagaa tcgagatagt aattataaga ggagctttaa
     5461 cggggagtgg aattagcaag acccacgctt tatttattgg cagaggttcg attcaagaaa
     5521 tgcacccaca cgggtgcatt tcttttttgg gcagttggga gaaatgaaat tgcgtgaaca
     5581 gatagagata ttggctacat tgcagaacgt tgatcgcgag ataagggaaa aaaacggcat
     5641 taagcaagaa ttactggggg agcttcaggt caaggacaaa gagatcctgt ctaagaagca
     5701 ggaaatcgcg ctgcttgcgg ctgcttgtgc ggaaaaagaa aaggaccgtc agggaaaaga
     5761 acgagtactg caagacgaag gcaaaaaggc catggataag cggatgcgaa tgaatcgcat
     5821 caagaacata aaagagctgc aagcgttaca gcgggagatt gatcaaatca aacagagtaa
     5881 cggggatctc gaggaagagc tgatcagggt tctcgaagag atcgaccgta tcaaggccga
     5941 gctcaaagct aaagaggaag aggcggccat gatggaggag gagtggcagc agaggcagga
     6001 agagatgcaa tcgaagctct ccggcattga tcaactcgtt tctgaagctt acacgcttag
     6061 aaaaacaatt gcatcgcaga ttgccggaga cctgattgca cgctatgagc tcatcttcgc
     6121 acggcgggga ggcagcgcgg ttgtggaggt tgccggtgga atttgccgcg gctgttatat
     6181 gaacataccg ccgcagctgt ggaatgaaat tatcaagagc gaaaaagtca atctttgccc
     6241 cagttgtcaa cgtattcttt attacaagcc gtctgcagaa caggaaaagg aagcctagcc
     6301 cagtcaagga tccggtctct ccattcgcaa ctcggcaatc attcgaagcg ccggctcacg
     6361 acgctgagct gggtttccat caagcaaaaa aatatcgcca tatacgtgtc tcttgaatta
     6421 tcggagagga gcgctccatt agctatgtcg gaactagaca acgaatggct cgtcatggtt
     6481 gacggcgctg cacgaggcaa ccccggacat gcaggatgtg gagctgtgat tcttgatgga
     6541 aacggcacgg tagtgaagga gttaagtcgt tacttgggac atgccaccaa caatgtcgca
     6601 gagtatgaag cgctgctcat ggggctggaa gcgctcttcc agctcggcaa gaggcgcatc
     6661 cgggtccaga gtgattcaca actactggtg cggcagctta atggtgaata ccgtgtgaag
     6721 gacgaaaagc taaaggtgct ttttcaaagg gcagtcacat tattgcgtca gtttgaggtc
     6781 tatcgtatcc ttcatgtgcc tcgggaaatg aacaagctag cggatcgatt ggctaacaaa
     6841 ggtatcgacg aggcaatcaa aagatcctca ctgggctaaa gtggatcaag cgatcgccct
     6901 ccgcctcaag cggagggagg aaagtccgga ctccacagga caaggtggcc gctaatggcg
     6961 gccccgagtg atcgggggaa agtaccacag aaaacatacc gcccattttt tatgggtaag
     7021 gttgaaaagg cgaggtaaga gctcaccggt ccgtcagtaa tgacggacgc ttggcaaacc
     7081 ccacccggag caagaccaaa taggagggtt gggtggtccg cccataaccc tcgggtaagg
     7141 tcgctcgacc ccgtgagcaa tcatggggca agatgaatga tcgctagggt tcctcttagg
     7201 aaccaataca gaatccggct tacgatccac tttagcccac aacacctgtc tcacactttt
     7261 ctgggcgcgc tttcgccaat tcgtgctaag gcccagaagg aatgtcctat cgcaaatttt
     7321 aggcactatc tgtcattctt tggcttttgt tgcggcccaa cgaggcgcta aaagttttga
     7381 ttcgatatct agtgtagcgg cctcccgtac cgtcaatata ttgtctcgat ctcaagtcgt
     7441 ggaaaagcac aaaaatccgc gaaaaatctg agatttttgt tgacagttcg cactcataaa
     7501 gttataagtg gcgcctacgt gggataatgt gggataattt cccattaaat taatttctga
     7561 cttaggcgac gcatgtttcg tggcagcttt gaacatacgc tcgatggcaa gggacgtttg
     7621 agcattcctg tgaagttccg ggaggttctt ttcgggaaaa gtgatgagcg catcgtgatt
     7681 acgaatttcg ttgtggatgg
//
LOCUS       DASSWZ010000044         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1331618, whole genome shotgun sequence.
ACCESSION   DASSWZ010000044 DASSWZ010000000
VERSION     DASSWZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331618"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFN58_01035"
     CDS             complement(<1..729)
                     /locus_tag="VFN58_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916600.1"
                     /translation="MVRAKKRYKVLVLFDTAGTPPQDQDFTKELKTDDWAAEAHVIGA
                     LKKLGHEVRTVGVWDEPGMIIDEIKTNPPDVVFNLTEHFNEVSAYDRNVAGLLEMMDV
                     PYTGSSPTGLTLCKNKGMAKELLAYHNIRVPNFAIFSPGSSIKRPKRLKFPLFIKPLR
                     EEGSVGISLDSFVDSDEAFEERICFIHERLNQEALAEEYIEGRELYVSILGTDRLRVF
                     PYREVIFAEVPEGQPKFSTFKAKWD"
     gene            complement(732..1787)
                     /locus_tag="VFN58_01040"
     CDS             complement(732..1787)
                     /locus_tag="VFN58_01040"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET8916601.1"
                     /translation="MDVSESKQKPESTRATAPLPELAGLSDDELLQMRICDLKLKIPG
                     TELESRIQTFYSELAAKGIAFKPLCYLGDEWFCPEGCATIAIPFYLAHPRLKRLEEKM
                     MMEVEGGTEAWCLSLLRHEMGHVLNHTYLLEKEKHWQKLFGPPSLEYSESFRARPYSK
                     RFVRHLGGWYAQSHPEEDFAETAAIWLTPDLDWRQQYKDWKALEKLEYVDSLMEKLAG
                     KQPLVFSKTKMSEASRLRSRLALYYKRRRHTYAQEFPDFFDADLKRLFVNSSASPNTE
                     RASTFLRRSTKPILNAVSAWTGEPKFTLHRLLRALADRCSELDLRLRSDAAGVEIAAY
                     LATLASHYRLTGKFKDS"
     gene            complement(1838..>2512)
                     /locus_tag="VFN58_01045"
     CDS             complement(1838..>2512)
                     /locus_tag="VFN58_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET8916602.1"
                     /translation="AVAKTSEVRPVPDGVTSTQAAATPTVFLTAWFGLLTDGDLKAGE
                     WLLVQGGSSGVGNAAIQIAKHLGAKVVTTSGSEEKCRRLRQLGADVTIDVSQNDFLPE
                     VLRVTNNRGVDVVLEMIGGDVYQKSLQALAPGGRLFSIGGAFGAIPDSPPALGDGRKA
                     TRFSITNYLKAKPDDFKQLDEILKLVQEKKFQVVIGKSFPLAETRAAQRYLEGREHFG
                     KVMLTM"
BASE COUNT          528 a          678 c          708 g          598 t
ORIGIN      
        1 gtcccacttg gctttgaagg tcgagaactt cggctgacct tcgggaactt cggcaaagat
       61 cacttcgcgg taaggaaaga cgcgtagacg atcggtgcct agaatgctga cgtaaagttc
      121 ccgtccctcg atgtattcct ctgcgagggc ttcctggttc agccgttcgt ggataaagca
      181 aatccgctct tcgaaggctt cgtcgctgtc gacgaaagaa tccagcgaga ttcccacaga
      241 gccctcttct ctgagcggct tgatgaagag cggaaatttg agtcgcttgg gtctcttgat
      301 gctcgagccg ggagagaaaa tggcaaagtt cggcacgcga atgttgtgat aggccagcag
      361 ttccttggcc atccctttgt ttttgcaaag agtcagtccg gtggggcttg agccggtata
      421 agggacgtcc atcatttcga gcaagccggc gacgtttcga tcgtaggcgg acacttcgtt
      481 aaaatgctcg gtgaggttga aaacgacatc gggaggattc gttttgatct cgtcgatgat
      541 cattcccggt tcgtcccaga caccgacggt tctcacttcg tgcccgagtt tcttaagtgc
      601 gccgataacg tgcgcttcgg ctgcccagtc gtcggtcttc aattccttgg tgaaatcctg
      661 atcctgagga ggagtgcccg ccgtgtcgaa gagcaccaaa actttatatc gttttttggc
      721 tcgaaccatg atcaagaatc tttgaacttg ccggttagac gatagtggga agccagggta
      781 gccagataag cggcgatttc gacgccggca gcgtcagaac gaagtcgcaa atccaattcg
      841 gagcagcggt ccgccaatgc ccggagcagg cgatggagtg tgaacttcgg ctcgcccgtc
      901 catgcggata cagcattcaa aatcggtttt gtcgagcggc gcagaaaggt cgatgcgcgc
      961 tctgtgttgg gcgaagcaga ggagttaacg aatagtctct tgaggtcggc gtcaaaaaag
     1021 tcgggaaatt cctgcgcgta ggtgtgtcgc cggcgtttgt aatacagcgc caatcgggat
     1081 cgaagccggg acgcctcgct catcttggtt ttcgagaaaa ccagcggctg tttccccgcg
     1141 agcttttcca ttaagctatc tacgtactcc agcttctcca gggccttcca atctttatat
     1201 tgttgcctcc agtccaaatc cggcgtgagc cagatcgccg cggtctcggc gaaatcttcc
     1261 tcgggatggc tctgcgcgta ccagccacca aggtggcgca cgaatcgctt gctatagggg
     1321 cgggcgcgga aactttcaga gtattccaac gacggcggac cgaataactt ttgccagtgt
     1381 ttttcctttt ccagcagata ggtgtgattc aagacatggc ccatctcatg acgtaaaagg
     1441 ctcaagcacc atgcttcggt gcctccctcc acttccatca tcatcttctc ttccagtctt
     1501 ttcagtcgtg gatgggcgag ataaaagggg atggcgatgg tcgcgcatcc ttcgggacaa
     1561 aaccattcgt cgccgaggta acagaggggt ttgaaggcga tccctttggc ggcaagctca
     1621 ctgtaaaatg tttggattcg cgattcgagt tcagtgccgg gaattttgag cttcagatcg
     1681 caaatacgca tctgcaaaag ctcatcgtcg ctcaagcctg ccagttccgg caacggagcc
     1741 gttgcccttg tcgactcggg cttttgcttg ctctccgaaa cgtccatccg gggctatttt
     1801 accagagcgg agagaaagca gctactcgcg cttatttcta catcgtcaac atgactttgc
     1861 cgaagtgctc gcgcccttcg agataacgct gcgccgcgcg ggtttccgcc aacggaaagc
     1921 tcttgccgat cacgacttga aacttttttt cctggacaag cttgaggatc tcgtccaatt
     1981 gtttgaaatc gtccggctta gctttcaaat aattcgtaat cgagaatcga gtcgcttttc
     2041 gcccatcgcc gagagccggc ggtgagtcgg gaatcgcacc gaaggcgcca ccgatggaga
     2101 aaaggcggcc gccgggcgca agtgcttgca aactcttttg atatacgtcg ccgccgatca
     2161 tttccaacac gacgtcaacg ccgcgattat ttgtcacgcg caggacttcc ggcagaaaat
     2221 cgttttggga gacatcgatg gtgacgtcgg cgcccagttg tcgcagtcgg cgacatttct
     2281 cttcgctgcc ggaggtggtc acgactttcg ccccgagatg tttggcgatt tggatcgccg
     2341 cgttgccgac gccgctgctg cctccttgca ccagcagcca ctcgccggct ttgaggtcgc
     2401 cgtccgtcaa caagccgaac cacgcggtga gaaagaccgt gggagttgcc gccgcctggg
     2461 tggaggtgac tccatcggga acgggccgga cttccgaagt cttggcgacg gc
//
LOCUS       DASSWZ010000045         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_157088, whole genome shotgun sequence.
ACCESSION   DASSWZ010000045 DASSWZ010000000
VERSION     DASSWZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157088"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFN58_01050"
     CDS             complement(<1..717)
                     /locus_tag="VFN58_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET8916603.1"
                     /translation="MLQEFKFPDVGEGIAEAEIIRWLVKEGDAVKEDQDLLEVETDKA
                     ILTLNSPYTGKVTKLHGKAGDIIKVGDVLTTFDAGGKAATVVEAEKKDSGTVVGTLSD
                     NEVVEVIRPVQATPAVRALAKQTGIDLARIKGTGPGGRITKEDVERAATKTTQQTNVE
                     ADTYGSVEKVPLRGIRRTVAKRMAEASKRVAEVTIWEDADITELEQVRAKERKVAEEK
                     GVKLTYLPFLIKAVIPALNGH"
     gene            complement(734..1711)
                     /locus_tag="VFN58_01055"
     CDS             complement(734..1711)
                     /locus_tag="VFN58_01055"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET8916604.1"
                     /translation="MAKLTMIEAINLALKQEMEKDPRVLILGEDVGKNGGVFRATQGL
                     FDLFGENRVVDTPLSESAITGVAIGMAVYGLRPIAEIQFEGFVYASMEQLANHAGRIR
                     TRSRGRYHCPLVVRLPYGGGIRAPEHHSDSNEAYFVHTPGIKVVVPSTPYEAKGLLIA
                     AIRDPDPVIFMEPKKVYRSLREEVPEEEYLIPLGQSRIVMEGPDVTLITWGAMLQPTL
                     QAATLMENRGIKAEIIDLRTLSPLDMQTVIESVEKTHKAVVVHEAPASCGVGAEVIAR
                     INEKALVHLEAPVARVAGFDTVMPLPKLEKYYLPNAERVAEAVDRVLNF"
     gene            complement(1724..2812)
                     /gene="pdhA"
                     /locus_tag="VFN58_01060"
     CDS             complement(1724..2812)
                     /gene="pdhA"
                     /locus_tag="VFN58_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET8916605.1"
                     /translation="MPKKTVQSFSVEWLQILDENGNCDEALRPPLSHEDIKKLYEWMV
                     LARVFDEKAFKLQREGRLGTYASILGQEAAQVGSAFALRPDDWMFPSFREPGASFVRG
                     LPMRMILQYWAGDERGSLIPEELNDFPITIPVGTQIPIAVGAAWAAKLKADQIAVLCY
                     MGDGATSKGDFHEGLNFAGVFAVPAVFVCQNNQWAISVPLKRQTAAKTLAQKAIAYGF
                     SGIQVDGNDIFAVYRATHEALNRARDGKGPTLVECVTYRLGDHTTADDASRYRSREEV
                     ELWKGRDPVERLKKYMEKEGLWNKSYDEAVRSEAREKVEQAVHEQENFPPPDPRDIFR
                     FTFHELTTELEEQLKSFVNTNGRRDIRD"
     gene            3286..4467
                     /locus_tag="VFN58_01065"
     CDS             3286..4467
                     /locus_tag="VFN58_01065"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET8916606.1"
                     /translation="MKKAVIVSAARTPIGSFNGVLSSVSATRLGSIAITEAVRRIVLS
                     GEEVDEVIMGNVLSAGLGQAPARQASLGAGLSKSVGCTTVNKVCGSGLKAVMLANQAI
                     LSGDAEVVVAGGMESMSNAPYLLMKARNGYRMGDGELIDSMIRDGLWDVYNRFHMGEA
                     AELCADTLGISRDDQDQFAATSYRRALEALQRGEFRREIVSVPVSQRNGADQLVEEDE
                     EPKRVSLERLPTLKPAFRKDGKVTAGNASSISDGAAALVVMLEEKAKGLGLTPRARIV
                     NYASFATDPAWFTTAPVHAISSLVKKTGRQISDVDLFEINEAFAVCSIAVNRELGLNP
                     DKVNVRGGAVALGHPIGASGARILTTLIHALEDRNLSTGVASLCIGGGEAVAMMVERE
                     I"
     gene            4528..>5160
                     /locus_tag="VFN58_01070"
     CDS             4528..>5160
                     /locus_tag="VFN58_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8916607.1"
                     /translation="MDFELAEEHQMVREMTRGFVEKEVKPVAPGMDRAGSYPSQLVKR
                     LSEMGLMGILVPQEFGGSGMDLLSCVVAMEEISKAWASLGVAMSVQNSLVCAPILRFG
                     SAAQKKKYLPSLASGKQLGCYALTEPGAGSDAGSIQTSAKKVADGFVLNGNKIFTTNG
                     NRADLAIVYAVTDPARGKKGISAFIVETKSPGFVVGKLEDKLGLRSSDTAS"
BASE COUNT         1093 a         1359 c         1420 g         1288 t
ORIGIN      
        1 atggccattc aaagctggaa taaccgcctt aatcagaaag ggaagataag taagtttcac
       61 tcccttttcc tctgctacct ttctttcttt ggcgcggact tgctctagct cggtgatgtc
      121 ggcatcttcc cagatggtca cctcggcgac acgtttagag gcctcggcca tacgcttagc
      181 gaccgtgcgc cgaatgcctc gaaggggtac tttttcgacc gagccatacg tgtctgcttc
      241 gacgttagtt tgctgagtgg ttttcgtcgc tgctctctcg acatcttctt tcgtgattcg
      301 tccccccggg cccgtgccct ttattctggc cagatctatt cccgtctgtt tggccaaggc
      361 acggacggcc ggtgtggcct gcaccggacg aatgacttcg acgacttcat tatcgctgag
      421 agtccctacc acggtgccgg agtctttttt ttcggcttca actacggtcg ctgctttgcc
      481 gcccgcgtcg aaggtcgtta agacgtcacc gactttgatg atgtcacctg ctttgccgtg
      541 gagtttggtg accttccccg tgtagggcga attcagggtc aggatcgctt tatcagtttc
      601 aacctctagc aggtcctgat cctcctttac tgcatcacct tcctttacaa gccatcgaat
      661 gatttccgct tcggcaattc cttcaccgac atcggggaat ttgaactctt gcagcatcgg
      721 aggctcccag gtgttagaag tttagaaccc gatcaaccgc ttcagcgact ctttcggcgt
      781 tgggcagata gtacttctcc agtttcggta gcggcatgac ggtgtcgaat ccggcgaccc
      841 ttgcaacggg tgcctctaga tgcaccagtg ctttttcatt gatccgggcg atcacttcag
      901 cgccaacacc gcagcttgcg ggggcttcgt gaaccaccac agccttgtgg gttttttcca
      961 cggattcaat caccgtttgc atatccaaag gcgatagggt cctcaagtct ataatctccg
     1021 ctttgattcc cctgttttcc ataagcgtag cagcttgcaa ggtaggttgg agcatagcgc
     1081 cccaagtgat caaggtaacg tcgggtcctt ccatgacgat acgtgattgc ccgaggggaa
     1141 tgaggtactc ctcctctgga acttcctctc tcaaagagcg gtagactttc ttcggctcca
     1201 tgaagatcac aggatcgggg tcgcggatgg cagcgatcaa aagacctttg gcttcgtaag
     1261 gagtcgaagg aacgacgact ttgattccag gcgtgtgaac aaaataggct tcgttgctat
     1321 ccgagtggtg ctccggcgct cgaatccctc ctccgtaagg aagcctgacg actaagggac
     1381 aatgatagcg ccctcgcgag cgggttctga tccgtccggc atgattagct agctgctcca
     1441 tggaggcata aacaaagcct tcaaactgta tttcggcgat gggcctcaag ccgtaaaccg
     1501 ccatgccgat tgcaactccc gtgatcgccg actcagacag gggtgtgtcc acgactcggt
     1561 tttctccaaa gagatcgaac aacccttgtg tggcccggaa aacccctccg tttttcccca
     1621 cgtcctctcc gagaataagc actcttggat ctttctccat ctcttgtttc aaggccaaat
     1681 tgattgcctc gatcatcgtc agttttgcca taaccacaga ccctcagtcg cggatgtccc
     1741 ttctcccgtt cgtattgacg aagctcttca actgctcctc aagttcggtc gtgagctcat
     1801 ggaaggtaaa gcggaatatg tctcgtgggt ccggaggagg gaagttttct tgttcatgaa
     1861 cggcttgttc tactttctct ctggcttctg aacggaccgc ttcgtcgtaa gacttattcc
     1921 aaagaccctc tttctccatg tactttttca ggcgttcgac tggatctctt cctttccaca
     1981 gttccacttc ttcgcggcta cggtaccggc tggcatcgtc tgctgtcgtg tggtcgccca
     2041 gccggtaagt tacgcattcg accagggttg gtcccttgcc atctcttgct cgattcagcg
     2101 cttcgtgggt cgctcgataa acagcgaaga tatcatttcc atcgacctgg attcccgaga
     2161 aaccatacgc gatggccttt tgggctaagg tcttggctgc ggtctgtcgt ttgagcggca
     2221 cggagatcgc ccattggttg ttctggcaga caaagaccgc tggcacggcg aagacgccgg
     2281 caaagttcag gccttcatga aaatctcctt tggaagtcgc gccgtcgccc atgtagcaca
     2341 acacggcgat ctgatcggcc tttagtttgg cggcccaggc ggcgccgacg gcgatcggga
     2401 tttgggtgcc tacggggatg gtgatgggaa agtcgttcag ctcttctggt attaagctgc
     2461 cgcgctcatc ccctgcccag tattggagaa tcatgcgcat cggcaggccc ctgacgaagg
     2521 aggctccagg ttccctgaag gagggaaaca tccaatcatc gggccgaaga gcaaatgcgc
     2581 tgccgacctg agcggcctct tgtcctagga tcgaagcgta ggtaccgagg cgtccttccc
     2641 gttgaagttt gaaagccttt tcgtcaaaga cccgggcaag gaccatccat tcgtaaagct
     2701 tcttgatgtc ctcgtggctt agcggcgggc gaagcgcttc atcgcaattt ccattttcat
     2761 ctaagatttg gagccactcg acgctaaaac tctgaacggt cttcttcggc attgagattt
     2821 cctcttgtcg acgtagtcga cccactcaga accagcatcg tatcttcaac attatattac
     2881 atcgcttcag gtttatttta aatatgttaa aataactttt gagaagtcac cggccgattg
     2941 gaggcgttcg atgcggctgt tggcaaacgt tttgcgatcg gccaaaattg cttttctatg
     3001 ctatcctttc cctagccgcg aggtcaccgc cgttttggat cgactcgcac ttagacccga
     3061 tatctgcggc atatcgaatt gctcgaattt agctctgttt caagtccaat tcgtttctcg
     3121 tgcgataagt ctaaataatc acatctccga tttttggccg agagcaaaaa aagaatgcgt
     3181 ccaccgttcg tttcgctctg ggatctgcga agaggttcgt tacgctgtga aatcgggtcg
     3241 ctcattagct gggtttgcgc cacgagtcca ggttaggaag agtctatgaa aaaagcagtc
     3301 atcgtcagcg ctgctcgcac tcctatcggg agctttaacg gtgttttgag ttccgtttcc
     3361 gccactcgtt tagggagtat tgcgataacg gaagccgtta gaagaattgt actgagcgga
     3421 gaggaggtgg acgaggtcat catggggaat gtgctttcgg cgggtttggg gcaggctccg
     3481 gcgcgtcagg caagccttgg cgccggtctg tctaaatctg tcggctgtac aacagtcaac
     3541 aaggtttgcg gctcaggctt aaaggcggtc atgctggcga atcaagcgat cttatcaggc
     3601 gatgccgagg tagtggttgc cggcggaatg gaaagcatga gcaatgcccc ctatttgttg
     3661 atgaaagcga gaaacggcta ccgcatgggc gatggcgagt taatcgatag catgatccga
     3721 gatggacttt gggacgttta caaccggttt cacatgggag aagcggcgga gctatgcgcc
     3781 gatacactcg gcatctcccg agacgatcaa gatcagttcg ccgcgaccag ttatcgccgc
     3841 gcgctcgaag cactacaacg aggcgagttc aggcgagaga tcgtttcggt gcccgtgtcg
     3901 caacggaacg gagcagatca gttggtggag gaagatgaag agccaaagcg cgtgagtttg
     3961 gaaaggctcc ccaccttaaa gcccgccttc aggaaggatg gtaaagtcac cgcaggaaat
     4021 gcgtcttcga tcagcgacgg cgccgccgcc ttagtggtca tgctggaaga aaaagcgaag
     4081 ggcctaggtc tcacccctcg agcgcggatc gtcaattatg cgtcttttgc tacggaccct
     4141 gcttggttca cgacagcgcc agttcatgcc atctcttctt tggtgaaaaa gaccggccgc
     4201 cagatcagtg atgtcgacct tttcgaaatt aacgaagcct ttgcagtctg ctccatcgcg
     4261 gtcaatcggg aattgggtct aaatccagac aaagtcaatg tccggggggg agcagtggcg
     4321 ttgggtcatc ctattggtgc cagcggcgcg aggattctaa cgacgctcat tcatgcgttg
     4381 gaggatagga atcttagtac aggagtcgct agcctttgca ttggcggcgg cgaggccgtc
     4441 gccatgatgg tggagcggga aatatgagtt tcgagttggg ggttgagcgt ctatgcagaa
     4501 actcgatacc cgaaaatcat aaccgacatg gattttgagc tcgcggaaga acaccagatg
     4561 gttcgggaaa tgacccgagg ctttgtggag aaggaagtca agccggttgc cccgggcatg
     4621 gaccgcgcgg ggagttatcc ttctcagctg gtcaaaagac tgagcgagat gggcctcatg
     4681 ggaatattgg tgccgcagga gtttggcggc tccggcatgg acctcctatc ttgtgtcgtc
     4741 gcgatggaag agatttccaa ggcatgggct tcgctggggg tcgccatgag cgtgcagaac
     4801 tcgctcgtct gcgcacctat acttcgcttc ggtagcgccg cgcaaaagaa aaagtacctt
     4861 ccttcgctag cgagcgggaa acagttgggt tgctatgcgt taaccgagcc gggcgcgggt
     4921 tcggatgctg gctccattca gacaagcgca aaaaaggtcg ccgacggctt tgtgctcaat
     4981 ggcaataaaa ttttcaccac caacgggaac cgcgcggacc tcgccatcgt gtatgccgtc
     5041 accgatcccg ctagaggcaa aaagggtatt tcggctttca tcgtggagac aaagagccca
     5101 ggattcgttg tcggtaagtt ggaagataag ctcgggctcc gctcgtcgga taccgccagt
//
LOCUS       DASSWZ010000046         8374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_666417, whole genome shotgun sequence.
ACCESSION   DASSWZ010000046 DASSWZ010000000
VERSION     DASSWZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666417"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..110
                     /locus_tag="VFN58_01075"
     CDS             51..110
                     /locus_tag="VFN58_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916608.1"
                     /translation="MLEGKERVEKIREQTQNIE"
     gene            107..772
                     /gene="gmk"
                     /locus_tag="VFN58_01080"
     CDS             107..772
                     /gene="gmk"
                     /locus_tag="VFN58_01080"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET8916609.1"
                     /translation="MIRKTKHLTRREGIIFILSAPSGTGKTTLVKRLGAVFPEIRLSI
                     SSTTRARREGEVHGRDYYFVNAEKFATMKAKGDFAEWAKVHGFFYATPRRPLDRCVRG
                     GRDILLDIDVQGASKIKRRYPRAVSIFLLPPSWRELQRRLARRGTDGKETIRRRLVNA
                     RGEIGEIIKYDYYVVNREVRTSVQLLKSIVEAERAKTFRVKQWRIEPLHRRQGDRGRE
                     QGN"
     gene            744..2978
                     /locus_tag="VFN58_01085"
     CDS             744..2978
                     /locus_tag="VFN58_01085"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET8916610.1"
                     /translation="MTVAVSKEIKITDLVDKVQSYYPAADVELIRKAYDFSAKVHQGQ
                     KRLSGEPYLIHPIAVAGVIADLKMDVPSVIGGLLHDTVEDTLTTLEELKSIFGREIAS
                     LVDGVTKLSRTNFSSREEKQAENFRKMLLAMGKDVRVILIKLADRVHNMRTLDHLPLE
                     NQILTAQETLDIYAPMAHRLGVAWIKSELEDLALKYLHPEIYYQLKRNVTKKKSDREK
                     YIAEVISVIKRKLEAEGIEADVTGRPKHFFSIYQKMESQNLLYDQIYDLVAFRILVDT
                     ARECYETLGVIHSQWRPVPGRFKDYIALPKPNMYQSLHTSVIGPYGERMEIQIRSHEM
                     HRVAEEGIAAHWRYKDGADFRVNDNQRFAWLRQLLEWQQNLQDPQEFLHSLKDDLFTT
                     ELYVFTPKGDLLNFPKGSTIIDFAYRIHSDIGHHCSGARVNGQLVALKYILRSGDTVE
                     IITTPQQVPSRDWLKLVKTPRAKSKIRDWLKSQQRERSVALGREILESDLHRYGLDYP
                     SLRKAGHIGSLTKELGLKDEEALLAALGYGRITPRHVLAKLVPQDKLDSGHKKKEGSL
                     ERLFRLVSGQKQGLGIRVKGIDDVLVRFALCCHPLPGEQIVGFITRGRGVTVHTVGCP
                     TVLESDPHRKIEVNWEEKSGQTPRAVKIEVTCVDQPGLLAAISSAITSAEANIARAQV
                     RTFSGQKALNTFDVMIKNSKHLQQVLQNISKVKGVYKAVRGRGRTGAEIRDPAGRNDF
                     SDAR"
     gene            complement(2988..3179)
                     /gene="rpmB"
                     /locus_tag="VFN58_01090"
     CDS             complement(2988..3179)
                     /gene="rpmB"
                     /locus_tag="VFN58_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET8916611.1"
                     /translation="MARVCSICGKGRSIGHNISHANNKTKRSWRPNLQRVRANFDGQV
                     KRALVCTDCIHSGRVQKVA"
     gene            complement(3185..3655)
                     /gene="rfaE2"
                     /locus_tag="VFN58_01095"
     CDS             complement(3185..3655)
                     /gene="rfaE2"
                     /locus_tag="VFN58_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET8916612.1"
                     /translation="MSDKIKSLDELKTLTAAARTNGKIVVFTNGCFDLLHRGHVHLLR
                     NAKALGDVLVVAVNSDLSVKLIKGPTRPILAETDRLELIASMKMVNYVTLFDAPDPYN
                     IISILRPDILVKGGDWSAEEVVGADLVKKNGGKVVSVPYLAGSSTTEIIAKIRS"
     gene            3814..5253
                     /locus_tag="VFN58_01100"
     CDS             3814..5253
                     /locus_tag="VFN58_01100"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8916613.1"
                     /translation="MEYQDLRDWIEIADEMGELKTLKGCDWNLEIGAVTELVAHKEDG
                     PAVLFEDIKGYPTGYRVLSNSLASRKRLALTLGLPAGETKMDFVKIWKDNYKNIKPVP
                     AKFVKDSPLFDNVYKDGDIDMFKFPTPFWHDKDGGRYIGTGSIDITRDPDEGWVNYGT
                     YRVMIHDKNKVGFYISPGKHGRMQRDKYAANGKPFKMAMSFGHDPLTFLAGSIEVPYG
                     VPEYEFIGGIRGEPFEMIEGEYSGLPIPANAEIVIEGDVHFDNPKVEGPFGEWTGYYA
                     SAERSEPWVEVKRLYHRNNPIILGSPPGRPPAELGWYRSYLRSALIWDEMEKAGVPDV
                     KGVWLTVSGGSRLMMVVSIKQRYPGHAKQAAVIASQCHAGAYLGRFVVVVDDDIDPSN
                     TDDVIWAMGTRCEPEQDIDILRRCWSGPLDPVIQVGKKGFNSRAIIDATRPYEWMKDF
                     PPVAESSKEVLDATAKKWGAELFGSNGKK"
     gene            complement(5279..6070)
                     /locus_tag="VFN58_01105"
     CDS             complement(5279..6070)
                     /locus_tag="VFN58_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HET8916614.1"
                     /translation="MKPSQWLFLSSLILLFPINLYAAQETNSVEARIKTLMLDPNTQS
                     PIVVLETVTDKKLLPIWIDVPEARAIALELEHVTTPRPLTHDLIRNIIQGLGATLQRV
                     VITDLRNNTYFAVLSLGFKGQDFQIDARPSDAIAVALRLKAPIYASTQVLAKSKSLPA
                     LTAPARELQKRLGIQAQDLTAEVAALLDISFQKGVLVVDVAKGSTAMDAGIQRGDIIT
                     KANDQTIQSTHELEALVQSKKPETRIKLEVIKKGKPTVIVIDLPS"
     gene            6215..7951
                     /gene="mutL"
                     /locus_tag="VFN58_01110"
     CDS             6215..7951
                     /gene="mutL"
                     /locus_tag="VFN58_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET8916615.1"
                     /translation="MKIHILSDEMASRIAAGEVVERPASVVKELIENSLDAGAAEIAV
                     SIEKSGTMLIRVTDNGEGMGPEDLTLAVERHATSKLRDEGDLFRVATLGFRGEALPSI
                     ASVAKMEITSRPPLSDIGYRIRVEGGKKDPPCIVGSSVGTSVEIKEIFFNTPARRKFL
                     KSPATELSHICDAVNRMALAHPDVHFRLSHDGRIIADYASVSQRMDRLHQVLGREIAR
                     DLIPFSSARGLLEISGFLSSAPASFPNTRYLMTFVNHRFVRDKVVSHAVLHGYETLLM
                     KGQYPAAIVFVEIPCSEVDVNVHPAKYEVRFRRPSDVHDAVAEAIRQALKQEAKGPSP
                     RALAMASAGFAGVMENSLPYLSPPFQPDKPLLKAEVFPMAAIPAYSTGEGFFSSMDIL
                     GQILGCYLVCFSARGLALIDQHAAHERVAFEKLRQQLNAGAVQKQSLLIPQAIELTAG
                     ELMLLEQELPTLERFGFLLEPFGPNSYAITSVPALLPEGDYRQIVQQMVAELAELEHS
                     EKLRQHLEERLATIACHSVIRANRLLETTEMRALLRDLDQIEFATQCPHGRPVMIEFS
                     RDDLERMFKRVV"
     gene            8057..>8374
                     /locus_tag="VFN58_01115"
     CDS             8057..>8374
                     /locus_tag="VFN58_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HET8916616.1"
                     /translation="MKPKIVVVLGPTAAGKSDVAMELALQMSGEIINADSQQVYRQMN
                     IGTAKPSRGECETVPHHLIDIVDPDEEFNVARYRELAAAGVHDIQKKGKPAIVCGGTG
                     LYIK"
BASE COUNT         2014 a         2087 c         2325 g         1948 t
ORIGIN      
        1 agctcaatac gatcagttca aaggtgcccc accttcccgt cgttcagtta gtgctcgaag
       61 gcaaagaacg ggtcgagaag atccgggaac aaacccaaaa tattgaatga ttcgtaaaac
      121 gaaacacctc acccggcgag agggaattat tttcattctc tcagctccct cagggacagg
      181 taaaacaacg cttgtgaaac gcttaggggc ggtttttccg gaaatccgac tctctatttc
      241 ctctaccacc cgggcccggc gcgaaggaga ggtccacggc cgtgactact atttcgttaa
      301 cgcggagaaa tttgctacga tgaaagccaa gggtgacttt gccgaatggg ccaaagtcca
      361 tggctttttt tatgcgacgc cgcgccgacc gctggatcga tgcgttcgtg ggggccgaga
      421 cattctgctg gacatcgacg tccagggggc gagcaagatc aaacgcagat atccacgggc
      481 agtctcgatt tttcttctac ccccatcatg gcgtgagttg cagcggaggt tggcccggag
      541 agggactgac ggcaaggaga cgatccgccg gcgcttggtt aatgcccggg gtgaaatagg
      601 agaaataatc aagtatgatt attatgtcgt taatcgcgag gttagaacat cggtgcaact
      661 tttgaaatcc atcgtcgagg ctgagcgagc caaaactttc cgagtaaagc aatggcggat
      721 cgagccattg catcggcgac aaggtgaccg tggccgtgag caaggaaatt aagatcaccg
      781 atctggtcga caaagttcaa tcttactatc cggctgcgga tgttgaactc atccgcaaag
      841 cttacgattt ttccgccaaa gtacatcaag ggcagaaacg gctgtcggga gagccctatt
      901 tgattcaccc gatcgctgtg gcgggtgtca ttgccgactt gaagatggac gtgcctagcg
      961 ttatcggcgg cttgctgcat gacacggtgg aagataccct gacaacgctg gaagaactga
     1021 aaagtatctt tggtcgagaa atcgcttcac tcgtggacgg cgttaccaaa ctcagccgga
     1081 caaatttcag cagccgggaa gaaaagcagg cggaaaactt tcgcaagatg ctgctggcca
     1141 tgggaaagga cgtccgcgtg attctcatca agctcgcgga ccgcgtccac aacatgcgca
     1201 ccctcgatca tttgccgctt gaaaatcaga ttctgaccgc ccaagaaacg ctggatattt
     1261 atgcgccgat ggcccaccgt ttgggcgtcg cttggatcaa gagcgagctt gaagacctgg
     1321 cgctcaagta tctgcacccg gagatctact accagctgaa gcgcaacgtt accaagaaga
     1381 agagcgatcg cgaaaagtac atcgccgaag tcatttcggt gatcaaaaga aaacttgaag
     1441 ccgaaggaat cgaggcggat gttactggcc gtccgaaaca ctttttcagt atctaccaga
     1501 agatggaaag ccagaacctt ctctacgacc agatctatga cttggttgcg tttcgaattc
     1561 tggtggatac ggcgcgtgag tgctatgaaa cgctgggcgt gatccattct caatggcgtc
     1621 cggtacccgg gcggttcaaa gactacatcg cgctacccaa gcccaatatg tatcagtcgc
     1681 tacataccag cgtgatcggt ccgtacggtg agcggatgga aattcaaatc cgcagccatg
     1741 agatgcatcg tgttgctgag gaggggatcg cggcgcactg gcgttataaa gacggtgcgg
     1801 atttccgggt taacgataac cagcgtttcg cctggctcag acaattgtta gagtggcagc
     1861 agaatctgca agatccccag gaatttttgc acagtcttaa agacgatctt tttactaccg
     1921 aactttatgt ttttacgccc aagggcgacc tgttaaattt ccccaaaggg tcaacgatta
     1981 tcgatttcgc gtaccggatt cattccgaca ttggtcatca ttgctccggc gcacgggtta
     2041 atgggcagtt ggttgctttg aaatacatat tgcgcagcgg tgataccgtg gagattatca
     2101 caacaccgca gcaggtccca tcccgcgatt ggttgaagct cgttaagacc ccccgcgcga
     2161 aatccaagat ccgcgattgg ttaaaatcgc aacaacggga aagaagcgtg gcactcggac
     2221 gagagattct cgaaagcgac ctgcatcgct acggattgga ttaccctagc ttgcgtaaag
     2281 caggtcacat cggttccttg acgaaagagc tggggctgaa ggacgaagag gcgcttctag
     2341 ctgcgctcgg ctatgggaga attacgccaa ggcatgtgct ggccaagtta gttccgcagg
     2401 ataaacttga tagtggccac aagaagaagg aaggttcttt ggagcggctt tttcggctgg
     2461 tgtcagggca gaagcagggt ttgggtattc gagttaaggg catcgatgac gtcttggtga
     2521 gattcgcgct ctgttgccac cccttgcctg gtgagcagat cgtcggtttc atcactcgtg
     2581 ggagaggcgt taccgttcat acggtgggtt gcccgacggt gctcgaaagc gatccgcatc
     2641 gaaagattga agtgaattgg gaagaaaaaa gtggtcagac gccccgtgcg gtgaaaatag
     2701 aagtcacctg cgtagaccag ccgggactct tagcggctat cagctccgcg attacaagcg
     2761 ccgaggcgaa tattgcgcga gctcaagtaa ggactttctc gggccagaag gcgctcaaca
     2821 ccttcgacgt gatgatcaag aattccaaac atctccagca ggtgttacag aatatctcaa
     2881 aggttaaagg ggtttacaaa gctgtccgtg gacgcggtag aacgggcgct gagatccgcg
     2941 accccgcagg caggaatgat ttcagtgatg cgcgataacc gcagcgctta tgcaactttc
     3001 tgaacgcgtc cggaatgaat acaatccgtg caaactaaag ctcgcttaac ctgcccgtca
     3061 aagttagctc taacccgctg cagattgggg cgccaagaac gtttggtctt gttgttggcg
     3121 tgactgatgt tgtggccaat ggagcgaccc ttgccgcaaa tcgaacaaac cctcgccata
     3181 gttgttaact ccttatcttt gcaataattt ctgtcgtcga ggagcccgcc aaatagggaa
     3241 cgctgacgac tttaccgcca ttctttttga ccagatcggc gcctacgact tcttcagcgg
     3301 accaatcgcc acctttaaca aggatgtccg gccgcaagat cgaaataata ttgtagggat
     3361 ccggcgcgtc aaacaacgtc acataattta ccatcttcat cgaagcgatc aactcaagcc
     3421 gatctgtctc cgctagtatc gggcgggttg gtcctttaat caacttgacg gataggtcgc
     3481 tgttgacggc gacaaccagc acgtccccaa gtgcttttgc gttgcgcagc aggtgaacat
     3541 gtcctcggtg gagcaggtcg aagcagccat tggtaaaaac tacaattttt ccatttgttc
     3601 tcgctgcagc ggtgagcgtc ttgagctcgt cgaggctttt aatcttgtcc gacacattca
     3661 atgggtaaca tgagaaactg gggttttcaa ttggaagttc ccgtgtttta aggaaattca
     3721 atgtcttgac cgttccggaa aatttcgtta gagtcggaag caattctgca aagccgcact
     3781 ttctacgcgc gttctcaaaa tcaggaggaa gaaatggaat atcaagatct tagagattgg
     3841 atcgaaatcg ccgatgaaat gggagagtta aaaaccctca aaggctgtga ctggaaccta
     3901 gaaattggcg ccgtcaccga gttggtggct cacaaagaag atggtcccgc cgttttgttt
     3961 gaagatatta agggttatcc aacgggctat cgggtattgt ccaattccct agcatcgagg
     4021 aaacggctgg cgttgaccct cggtcttccg gcaggcgaga ccaaaatgga ctttgtcaaa
     4081 atctggaagg acaactacaa gaacatcaaa ccagtcccgg cgaaatttgt taaggatagc
     4141 ccgctattcg ataacgttta caaagacggc gacatcgata tgtttaagtt cccaacgccg
     4201 ttttggcacg acaaggatgg tggccgctac atcggcaccg gaagcataga tatcacgcgc
     4261 gacccggacg aagggtgggt gaattacggc acctaccggg tcatgattca tgacaagaat
     4321 aaggtgggat tctatatttc accgggcaag cacggccgca tgcaacgaga caaatacgcc
     4381 gccaacggca agcccttcaa gatggctatg tcgttcggcc acgatccgct aaccttcttg
     4441 gccggtagca tcgaagttcc ttacggcgtt cccgaatatg agtttattgg cggtattcgt
     4501 ggcgaaccgt tcgaaatgat cgaaggcgaa tactccggat taccgattcc cgcaaacgcc
     4561 gagatcgtca ttgagggtga tgtgcatttc gataatccaa aggtcgaagg cccattcggt
     4621 gaatggacgg gttattacgc tagcgcggaa cggtcggaac cctgggtaga agtgaagcgg
     4681 ctgtaccacc gcaacaatcc gatcatcctc ggttcccctc ctggccgtcc tcctgcagaa
     4741 ctcggttggt accgctctta cctccgttcc gcgttgattt gggatgagat ggagaaggcc
     4801 ggcgtgcccg atgtcaaagg cgtttggctt accgtttcgg gtggcagccg gttgatgatg
     4861 gtggtttcga tcaagcaaag atatccgggc cacgccaagc aggccgcggt gatcgcatcg
     4921 caatgccatg ccggcgccta tcttggacgt ttcgtggtgg tggtcgacga tgacatcgat
     4981 ccgtcgaaca cggacgacgt catttgggca atgggtaccc gctgcgaacc cgaacaagat
     5041 attgacatcc tccgccgctg ctggagcggg ccgctcgatc ccgtcattca agtgggaaag
     5101 aaaggcttca actctcgcgc aatcatcgac gcgacgcgcc cgtatgagtg gatgaaagat
     5161 ttcccaccag tggctgaatc gagcaaagaa gttttggatg ccacggccaa gaagtggggt
     5221 gctgagcttt tcgggagcaa cgggaaaaag taagatccgc tacgagtttg aattgccgct
     5281 acgagggcaa gtcgatcaca atcacggtag gcttgccctt ttttatgact tccagtttga
     5341 ttcgggtctc aggttttttg gactggacca gcgcctctag ctcatgggtt gactgtatag
     5401 tttgatcgtt ggcttttgtg atgatgtctc cacgttggat ccccgcatcc atcgcggtgc
     5461 ttcccttcgc tacgtcaact acgagaacgc ccttttgaaa tgagatgtcc agaagcgccg
     5521 cgacctccgc ggttagatcc tgcgcttgga tgcccagccg tttttgtagc tctctggcgg
     5581 gggcggttag cgccggcagc gacttggatt tcgccaatac ctgagtcgac gcgtagatgg
     5641 gagctttcaa tcttaaagcg acggcgatag cgtcgctcgg ccgcgcatcg atttggaagt
     5701 cctgaccttt gaatcccaaa gacaggacgg cgaaataggt attgttccga aggtcggtaa
     5761 tcaccacgcg ttgtagtgtt gcacccaatc cttgaatgat attgcggatc agatcatgag
     5821 tgagagggcg tggtgttgta acatgttcga gttcgagggc gatggctctc gcttccggca
     5881 cgtcaatcca gatcggcaag agcttcttgt cagtcacggt ctcaaggacg acgatcgggg
     5941 attgtgtatt cggatccagc atgagggttt taattcttgc ctccacggag ttggtctctt
     6001 gtgcagcgta gaggttgatg ggaaacaaaa ggatcagcga ggacagaaaa agccattggc
     6061 taggtttcat gcaacctcct tcgccagaat tgtggctgtc accgaaggtc aacttcacct
     6121 tatctgcgcg ctttggatca tgtcaatggt gccgtttttc ttgaccgggt cattggcgct
     6181 gagttattgt cacttatagg agagtcttgc ggcagtgaaa attcacattc tctcggatga
     6241 aatggccagc cgcatcgccg cgggagaggt tgtcgagcgt ccggcgtcgg tagtcaagga
     6301 gctgatagaa aattccttgg acgctggtgc cgctgaaata gccgtctcta tcgaaaagag
     6361 tggcacaatg cttattcgtg tcactgataa tggcgaaggt atgggcccgg aagatctcac
     6421 cctcgcggtg gagcggcatg caaccagcaa acttagggac gaaggggatc tttttcgcgt
     6481 cgccactttg ggattcaggg gggaggcgct accgagtatc gcatcggttg caaagatgga
     6541 gatcacctcc cgcccaccgc tttcggacat cggttaccgg atacgagtcg aaggaggcaa
     6601 aaaagacccg ccttgcatcg tcgggtcgtc ggtgggaacc agcgtcgaga tcaaagagat
     6661 ttttttcaat actcccgcac gccggaagtt tctcaagtcg cccgctaccg agctgagcca
     6721 tatttgcgat gccgtgaatc gtatggcttt agcgcatccg gacgtacact ttcgcctgtc
     6781 ccatgacggc aggatcatcg ccgactacgc aagcgtaagt cagcgcatgg accggctgca
     6841 ccaggttctg gggcgcgaga tcgcaagaga cttgatcccg ttctcgtcgg ctcgaggact
     6901 gttggaaata agtggattct taagttccgc gccggcctcg tttcccaaca ctcgttatct
     6961 catgactttc gtcaatcacc gttttgtgcg cgacaaagtg gtaagccacg cggtgcttca
     7021 tggttacgag actttgttga tgaaggggca gtatcctgcg gccatcgtgt ttgtagaaat
     7081 cccttgctct gaagtcgatg tgaatgttca tcctgcaaag tatgaagttc gttttcgccg
     7141 gccgtcggat gtccacgatg cggtcgctga agcgattcgg caggcattga aacaggaagc
     7201 caaggggccg tcgccacgcg cgctggcgat ggcatccgcc gggtttgccg gcgttatgga
     7261 aaattcgctt ccgtacttga gtccgccgtt ccaaccagac aagccattgc taaaggcgga
     7321 agtgtttccg atggccgcga tccccgcata ttcgaccggc gagggatttt tctcctccat
     7381 ggatattctc ggacagattc ttggatgtta cctggtttgc ttttcggcgc gggggttagc
     7441 actgattgac cagcacgccg cccacgagcg agtcgccttt gagaagttac gccagcagct
     7501 gaacgccggc gcagtccaga aacagagcct gctcatccct caggcaattg agttgaccgc
     7561 gggagagttg atgctcttgg aacaagaact tccaaccctg gagcgatttg gctttctcct
     7621 ggaacccttt ggtcctaatt cctatgctat tacgagtgtt cccgcgttgt tgcccgaagg
     7681 agattaccga caaatcgtcc agcaaatggt tgcagagttg gcggagctgg agcattcgga
     7741 gaaactccgc cagcatctgg aagagcggtt agccaccatc gcttgccaca gtgtgatacg
     7801 ggcaaatcgt ctactcgaaa cgacggagat gcgggcgtta ctcagagacc tcgatcaaat
     7861 tgaatttgcc acccaatgtc cccatggtcg tcccgtaatg atcgagttca gccgcgatga
     7921 cttggagaga atgttcaaac gagtagtgta gtctaaggag ttctgggttc aatgtgcaag
     7981 gttgcgggcc ccaggttctg actattaacc ttaaacgttg aaccttgaac cgtagagacc
     8041 tcgtctctag ttttccatga agcctaaaat cgtcgttgtc cttggcccga cggccgcagg
     8101 caaaagcgat gtcgcgatgg agcttgcctt gcagatgagt ggcgagatca tcaacgccga
     8161 ctcgcaacag gtctatcgcc agatgaatat cggaacagcc aaaccttcta ggggagaatg
     8221 cgagacggtt ccgcatcatc ttatcgacat cgttgatcca gacgaagagt ttaatgtcgc
     8281 cagatatcgg gaactagcgg cggccggcgt ccatgatatt caaaaaaaag gtaaaccagc
     8341 gatcgtttgc ggtggaaccg ggctttacat caag
//
LOCUS       DASSWZ010000047         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1274982, whole genome shotgun sequence.
ACCESSION   DASSWZ010000047 DASSWZ010000000
VERSION     DASSWZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274982"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFN58_01120"
     CDS             complement(<1..696)
                     /locus_tag="VFN58_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8916617.1"
                     /translation="MVWGVPYALLIAFGAVPLILLLHSLKPKGFKVSTTTLFLWERVL
                     KQRPLATRIGWLFRKNLLLILQILAACLLIAALADPSLLHFGAPSGDMVVVVDLSASM
                     KAGGPSGTRFEAARKEFLSLVDDLSSGQKMMLIGAGAQTRLIVPFTADKQRLRDAGRS
                     MVATDVSGRIKEAILFAHAFLKRGSPDHVVVISDGAFAGAEDFARQAAHLRFIKVDGG
                     RDNVGIVGFEVRRH"
     gene            complement(700..1734)
                     /locus_tag="VFN58_01125"
     CDS             complement(700..1734)
                     /locus_tag="VFN58_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET8916618.1"
                     /translation="MSFESEVRGVLYERWRKILRFAQDDKRQRAQNNRIVDAQKFATV
                     ASEIQERKPETFFDAEFLKKLERLRLIAKRLSWANAKGEHPSSRRGFSLEFSDYRRYQ
                     QGDDLRYVDWNIYRRLDRLLLKVFTADEEMNVYLFVDTSRSMAEGTPPKIDYAKKVAA
                     ALGYIGLKNLDRVGGAAFSSALQAPLTLGRGRKQVLSMFSFLSKLSCHGETNLRSAAH
                     SFTNLFPHPGLVVIVSDLFDPAGWRAAIEELAIKKYQLLVIHVLDDQEINPRPWGDVA
                     LNDVENRRERRLFLDVDLVRRFQQELTDYFKEIEMVCSSRRIDYLRTTTQIPFDEFVL
                     QTLRQVSSVT"
     gene            complement(1747..2754)
                     /locus_tag="VFN58_01130"
     CDS             complement(1747..2754)
                     /locus_tag="VFN58_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8916619.1"
                     /translation="MEAVAEIEERMQRARRSFDDLKAEMARIIVGHEDLIEQVFIALV
                     CGGHCLLEGVPGLGKTLMVRSLGEILSLSFSRVQFTPDLMPADITGTNVLTDDPSGRK
                     IFEFQRGPVFAHIVLADEINRATPKTQSALLEAMQEQSVTVGGKVHRLENPFMVLATQ
                     NPIEMEGTYPLPEAQVDRFFFKLLLKYPTRDELARIADFTTAGQPAALREVLDTADIL
                     QMRQWVRDVPLADIVKDYAVRLVLNTHPQREPGKEAAPLAQKFVLYGSSPRGLQSVIL
                     AAKARALLAGRPNVSFEDIRQMLLPSLRHRLILNLEADAEGIGAEKILSEVVERVAEV
                     S"
     gene            complement(2798..4222)
                     /locus_tag="VFN58_01135"
     CDS             complement(2798..4222)
                     /locus_tag="VFN58_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916620.1"
                     /translation="MSSEAETLRKKLAELLSWERRKRREQILATLFCYALLAALLTLP
                     FHGFLETVISRWFIPISFFGLMAPFFLGRGRWRSSDSARALARVDKTLRLDERALTAW
                     ELLERNETRAAALMVLKQAGEKLTNLDPKALFRRSWSWQAYCALPLLLLWLGLVWFDV
                     GLRFDSGVQLSAPKTVAQKLREFSRELQEKAKNEGLHESFRVGRELEQVAQKGIDAKT
                     GDEKFKADLAGMQNKIATMGKSAAEQQSLSAAESQQDLRDLKAELEAARDLLNMADAG
                     KGAQELGKQWLDQLATLPQLKKQFEREGQAGQSLSQNELKSFLDKLEKQATGELDRRT
                     LLDAQQFLEQLRKQGQGEKGESDARMAGRGERDSPDEGERTNNRSNLPGKEPGKRETE
                     SPSLPEFQPGAAAHVKGLLGEGNSSGLVLKAKPSAGKSEVSQDEVIASYRRQAEVELN
                     TERVPEALKETIKNYFLSLGIGKE"
BASE COUNT          929 a         1328 c         1140 g          974 t
ORIGIN      
        1 atgccgccgc acctcgaagc cgacgatgcc cacgttgtcg cgcccgccgt cgaccttgat
       61 gaagcgcagg tgcgcggctt ggcgggcgaa atcttcagcg ccggcgaaag ccccgtcgct
      121 gatcacgacg acatgatccg ggcttcccct tttgagaaac gcatgggcga aaagaatcgc
      181 ttctttaatc cgcccggaca catccgtcgc gaccatactt cgtcccgcgt cacggagcct
      241 ttgcttgtcc gcagtgaaag gaacgatgag acgggtctgg gcgccggcgc cgatgagcat
      301 catcttctga cccgacgaca ggtcatcaac caaggacaga aactcttttc gcgcggcctc
      361 aaagcgagtt cccgagggac ctcccgcctt catgctggcg ctcaagtcga ccaccaccac
      421 catgtcgcct gacggcgcgc cgaaatgaag cagcgacggg tcggccagcg ctgctatgag
      481 aaggcaggct gctagaattt gcagaatcag cagcagattt tttctgaaca gccagccgat
      541 ccgcgtcgcc agcggccgct gcttcagcac gcgctcccaa agaaagagcg tcgtggtgct
      601 tactttaaat cctttgggct tgaggctatg cagaaggaga atgagaggga cagccccgaa
      661 agcaattagg agcgcgtatg gaacacccca gaccatagtt tacgtcacgc tgctcacttg
      721 ccgtagcgtc tgcagcacaa actcgtcaaa ggggatttga gtcgtcgtcc gcagataatc
      781 gatccgacgg ctggaacaga ccatttcgat ctccttaaaa tagtctgtca gctcttgctg
      841 aaagcggcga accagatcca cgtccaaaaa caatcggcgc tcccgtctgt tttccacatc
      901 gttcagcgcc acatcgcccc agggcctggg attgatctct tgatcgtcca aaacatgaat
      961 caccaggagc tgatattttt ttatcgcgag ttcctcaata gcggcgcgcc agccagcggg
     1021 atcgaacaaa tcgctcacga tgaccacgag gccgggatgg gggaagagat tggtgaagga
     1081 gtgagccgcc gatcgcaggt tggtctcgcc atggcacgat agcttactga ggaagctaaa
     1141 catactgagc acctgctttc gtcctcttcc caaggtaagg ggagcttgca gggcagatga
     1201 aaatgccgcg cccccgaccc ggtccaaatt cttgaggccg atgtaaccca acgccgcggc
     1261 aacttttttg gcatagtcaa tcttcggcgg cgtgccttcg gccatggacc ggctggtatc
     1321 gacgaacagg taaacgttca tctcctcatc ggcggtaaaa accttcagca gcagccgatc
     1381 gagccgccgg taaatattcc agtcgacgta gcggaggtca tcgccctgtt ggtatctgcg
     1441 gtagtcggag aactcgaggc tgaagcctct tcgggatgag ggatgctcgc ccttggcgtt
     1501 ggcccaactc agccgcttgg cgatcagtcg cagccgctcg agctttttca aaaactccgc
     1561 gtcaaagaag gtttcaggtt tccgctcttg gatttcggac gcgacagtag caaatttttg
     1621 agcgtctacg atccggttat tttgagcccg ttgccttttg tcatcctgag cgaagcgaag
     1681 gatctttctc caacgctcgt acaaaacacc gcgtacctca gactcgaaac tcatgaactt
     1741 tccgtcctat gaaacttcag caacccgttc cacgacttcg ctgagaatct tctccgcgcc
     1801 gatcccttcg gcatcggctt cgagattgag aatcagccga tggcgcaagc ttggcagcaa
     1861 catctgccga atgtcttcga acgaaacatt cggccttccc gcaagaagcg cgcgggcttt
     1921 ggccgcaaga atcacactct gcagtcctct ggggctggag ccgtagagga caaacttctg
     1981 cgctaacggc gctgcttcct tgcctggctc gcgctgcgga tgcgtgttca gcaccaaccg
     2041 aaccgcatag tccttcacaa tgtcggcgag aggcacatcg cgcacccatt gtctcatctg
     2101 caggatgtcc gctgtgtcca ggacttcgcg cagtgccgcc ggttgtcccg ccgttgtgaa
     2161 atcggcaatg cgagccagct catcgcgagt cgggtattta agcaaaagct tgaagaagaa
     2221 acgatccacc tgggcttcag gaagcggata ggttccttcc atctcgatag ggttctgtgt
     2281 ggccagcacc atgaacggat tctccagacg gtgcaccttc ccgccaacgg ttacactctg
     2341 ctcctgcatg gcttccaaca gggcggattg agtcttgggc gtagcgcgat tgatttcgtc
     2401 ggccagcaca atatgggcaa aaaccggacc gcgctggaac tcaaagatct ttctcccgga
     2461 cggatcgtcg gtaagcacgt tggtgcccgt gatatcggcg ggcatgagat caggcgtaaa
     2521 ctgtacgcgc gagaaactaa gactcagaat ctcaccgagg ctacgcacca tgagcgtctt
     2581 gcccaatccc ggcaccccct ccagcaggca gtgaccgccg cagacaagcg cgatgaaaac
     2641 ctgttcgatg agatcctcat ggccgacgat gatgcgcgcc atctccgctt ttagatcatc
     2701 gaaagaacga cgggcgcgtt gcattcgctc ttcgatttct gccaccgctt ccatcaagtg
     2761 aattctcctt tagggtggat cccaaaaaac actcattcta ctctttcccg attcccaggg
     2821 agagaaagta gttcttaatc gtttctttca gcgcctctgg cacccgttcc gtgttgagct
     2881 cgacctccgc ctggcgtcga taggaggcga taacttcgtc ctgtgacact tcgctcttgc
     2941 cggccgaagg ttttgccttt aagaccaagc cgctactgtt gccctcgccg agtaagcctt
     3001 tcacgtgggc cgccgctccc ggttgaaatt ccggaagaga tggcgattcc gtctctcttt
     3061 tgccaggctc ctttcccggc aaattacttc tattgttcgt cctttcacct tcatcgggtg
     3121 aatctcgctc tccccgtccg gccatccgcg catcgctttc acccttttct ccttggccct
     3181 gtttcctcag ttgctcgaga aactgctggg catccaacaa ggtccgccgg tccagttccc
     3241 cggtggcctg cttttccaat ttatcgagga aagacttgag ctcgttctga ctgaggctct
     3301 gtcccgcttg accttctcgt tcgaattgct ttttgagttg gggcaacgtc gccaattgat
     3361 cgagccactg tttgcccaac tcctgcgccc ctttgcccgc atcggccata ttcaagagat
     3421 cccgcgctgc ttccaattcg gctttcagat ccctaaggtc ttgttggctc tcagcggctg
     3481 acaatgactg ttgttcggcg gccgattttc ccatcgtggc gatcttgttt tgcattccgg
     3541 ccagatcggc tttgaacttt tcatcgccgg ttttcgcgtc gatacccttc tgcgctacct
     3601 gctccaactc tcgacccact cggaagctct catgtagacc ctcgttcttc gctttctcct
     3661 ggagttctcg agaaaactct cgcagcttct gcgccacggt cttcggagca gaaagctgca
     3721 caccgctgtc gaatcgaaga ccgacgtcaa accacacaag tccgagccaa aggagaagaa
     3781 gcggaagagc gcaataggcc tgccagctcc agcttctgcg aaacagtgcc ttaggatcga
     3841 ggttcgtgag tttctccccc gcctgtttta aaaccatgag cgccgccgct cgcgtctcgt
     3901 tgcgctccaa caactcccac gcagttaggg cgcgctcgtc gagccgcagc gtcttgtcaa
     3961 cccgcgccag cgcccgcgcg gaatccgacg accgccaacg ccctctgcca aggaaaaacg
     4021 gcgccattaa cccgaaaaat gaaatcggaa tgaaccaacg gctgatcacc gtctccaaga
     4081 agccatgaaa cgggagggtg agcaacgcgg ctagcaatgc gtagcaaaaa agtgtggcga
     4141 ggatctgctc gcgccgcttt ctccgttccc agctcagcag ctcggcgagt ttcttccgga
     4201 gggtttctgc ttcggaactc aaacggttaa atccttcctc agcgcccagc ggtgcgctgc
     4261 taaaccgatg ctgccaatta gcaagtgaaa tgtaaaatgg acggtggtcg caggtacctt
     4321 ccatccaccc atactgagcg gcggcaactc ctgtcggccg atataggcga g
//
LOCUS       DASSWZ010000048         8631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_864698, whole genome shotgun sequence.
ACCESSION   DASSWZ010000048 DASSWZ010000000
VERSION     DASSWZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864698"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /gene="mutS"
                     /locus_tag="VFN58_01140"
     CDS             <1..1513
                     /gene="mutS"
                     /locus_tag="VFN58_01140"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET8916621.1"
                     /translation="PRDLAAIRETLTHVGAVRRELDPCNSTILKSLQTKIAELPEVVL
                     LIESAIVDAPPLSVKEGGFIRAGFDAELDEVRGMRAHAKEWIAAFEAGERRRTGIQSL
                     KVRYNRVFGYYIEVTRSHLKAVPEDYIRKQTLANGERYITPELKEYEAKILNSQELIE
                     KLEIALLARVREQVAVHFIALKEMSVALAMLDVLLSFAEVAESQHFTRPRVNNGFALI
                     IREGRHPVVEATLGRGGFVPNDSILDPASNQILLLTGPNMAGKSTYMRQVALIVILAQ
                     MGSFVPATEARIGIADRIFTRIGATDSLARGESTFMVEMKETANILQHATERSLILLD
                     EVGRGTSTFDGISIAWAVAERLHDMPGRPRTLFATHYHELTDLARTKERIRNFNFSVK
                     EWQGDVIFLRNLVEGAASHSYGIHVGRLAGLPPAVIERAKQILQNLESGEAEGKRADL
                     EKREVDVDEPVQMALFGSVERKLGEELKKLDVSRLSPIEALNLLHQWSEEAKK"
     gene            1535..2857
                     /locus_tag="VFN58_01145"
     CDS             1535..2857
                     /locus_tag="VFN58_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET8916622.1"
                     /translation="MAKRLLLGIFGLLHLGLWVPQGCAGGTAAPHRPAQVQKKAPATS
                     PSASPANNEALEVDRVAQSTKVSASPDRSRLFSVRVSSSQTYSRVTMDLSHEVRYETH
                     RLKEDPSKGLPPRIYVDLFSTKLAMDARNPIAADDGVLRQLRVGQFTADVVRIVIDLT
                     TSGEHNAFLLADPYRLVIDIQGQKNSEAVAAADKSKKKTATLPERAKPLPVPAIRKVV
                     LDPGHGGKDPGAIGVGGIAEKDIVLAVAKKLERKLKQEMGVEVVLTRRDDRFIPLEDR
                     TAIANAEGADLFVSLHMNASPNGEARGLETYYLDNTNDEGSLRLAARENGTSRRNVSD
                     LQFILSDMTQNMKLEDSITLAHHLQHSLVDGMSTRLTDVKDLGVKKALFYVLVGARMP
                     SVLVEMFFITNKTEGRAMSQESYQNAVVDALYDGILKYKESNVASKTL"
     gene            2871..5579
                     /gene="glnD"
                     /locus_tag="VFN58_01150"
     CDS             2871..5579
                     /gene="glnD"
                     /locus_tag="VFN58_01150"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET8916623.1"
                     /translation="MDAIALSRSLLDETQAESDLRRAARSYVKKGRELLLEKHRAGAA
                     GVEVVSVYSTMMDQLIRYLFSKVSDDFIRRYPSENPRCTVVAQGGYGRGELNPYSDID
                     LLFLYSWKISPYVEAVTERLLYTLWDAGLQVGHATRTIKESIALSASDMKVKTALLDA
                     RYLCGHYELYQEFDKAVENRLVNKRVSRFVQEKLTESRLRHESYGGSVYLLEPDVKDG
                     EGGLRDIHTARWIGRVKAGAKDLDGLALKGIVNPDDVAKLKASQDFLLRVRNELHFST
                     GKHQDQLTFEEQQKVSQALGFEGEGTLKAVEVFMRNYYLHAAQISRLASLIIHRLTDA
                     PRPLVGGLYSFARTLREGICVSHGQLFVTKPSILKSEPGNLIDVFAEMQKHRCELSHE
                     TRELLREHIGLIDDRFRRSAAANMPFFKILKWKERVYETLLEMYRCGILGAFIPEFGR
                     LLCMVLHDAYHIYTVDQHSLKLIQEIERLKAGAYKEGMPLLTQLARDADKIELLYLGL
                     MFHDIGKGFGGGHSEIGARLVSPIARRMRLNADDGSLVEFLVRHHLLMTHTAFRRDLE
                     DEKTIFDFAKTMGNVNNLKMLYLLTFADVKAVGPDVWNPWKASLLGELYVKTLNVLEE
                     MEKGEFQREDVRAGLRRIQARVQRLLAKDHPADRVDHFLESMPERYFLSTAESDIPAH
                     FELMEKFRGKGAVTAVEHFPERDCSSIVICTQDRPGLFASITGTLTALNLDILNARIF
                     TSSDGRILDVFRVSHRGRSELVMAEQKWSKFRSTLDDVLEGRLDVARLVENSRRAPFL
                     QRHVPKVPTVVQVDIEASDNFTIVEVFTEDRIGVLFKITYALHQLGLSIHVAKISTNV
                     DQVADIFYVADENGRKIEEPGRLEEIRLSLYESLAPQDERVAQSSH"
     gene            5551..6477
                     /gene="xerD"
                     /locus_tag="VFN58_01155"
     CDS             5551..6477
                     /gene="xerD"
                     /locus_tag="VFN58_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET8916624.1"
                     /translation="MKESLSPHIDSFLTMIAVEKGLAKNTVEAYSRDLVRLSEFLVAQ
                     SVASWEETETLHLRSYLSSLRRNGLSPRSIARHIVTVRRFYGFLETEGVINENPVPPF
                     HLAAGGRKLPQTLSGDDVRKLLDQPNPQELLGVRDQAMLELLYATGLRVSELISLQMQ
                     QINFQGNFLTVKGKGSKVRAVPFGKWARQKLLDYVNGARRRLAKGRASVFVFINRSGR
                     PLSRQGFWKLIRGHARAAGVEKRVTPHTLRHSFATHLLEGGADLRSVQSMLGHADIST
                     TQIYTHVNGARLKEVHRRHHPRERGDRKQDLE"
     gene            6630..7301
                     /locus_tag="VFN58_01160"
     CDS             6630..7301
                     /locus_tag="VFN58_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET8916625.1"
                     /translation="MVDAIQQIALWALPVLVAIIFHEVSHGWVAYRLGDPTAARMGRL
                     TLNPIAHIDPIGTILLPALLIISNAPFLFGYAKPVPVNFHNLRNPKRDMVWVALAGPV
                     TNLVLAAGCIVVLKIGLPLIAAGAIYSSSSGVVNFLAPVALMAKNGVLINIVLAVFNA
                     LPLPPLDGGRVAVGLLPEPYSSMLARAEPYGFLILIILLMTHALDKIIGPPTEFLINF
                     YFKFL"
     gene            7313..8290
                     /gene="trpS"
                     /locus_tag="VFN58_01165"
     CDS             7313..8290
                     /gene="trpS"
                     /locus_tag="VFN58_01165"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET8916626.1"
                     /translation="MDTIVSGMRPTGRLHLGHLHGALKNWLRLQENYRCYFFVADWHA
                     LTTDYASPQGIKPSTSEMVTDWLSVGLDPRRSTIFRQSQVKEHAELHLLFSMITPVPW
                     LERNPTYKDQLKELVEKDLSTYGFLGYPVLQAADITIYKANKVPVGVDQAPHVELTRE
                     IVRRFNQIYRPIFPEPEVLLTEAQKVPGLDGRKMSKSYNNAVFLSDTPNEIDQKLSRM
                     MTDPARVRRTDPGEPEKCPAFQLHKIYCTPEEIAEVSTGCRTAGIGCLDCKKVMIKHV
                     IEDLAPFREQRARYEKDPEQVEAVLTTGNAIAHEKAAETMVEVREAVGL"
     gene            8287..>8631
                     /locus_tag="VFN58_01170"
     CDS             8287..>8631
                     /locus_tag="VFN58_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET8916627.1"
                     /translation="MTTRVQLEIFEGPLDLLLHLIKKNEVSITDIPIATITEQYLATL
                     EVMQAFNLDVAGEFLVMAATLIHIKSRMLLPMTDDEDDEEEGTDPRQELVRRLLEYQR
                     FKDAADQLERREL"
BASE COUNT         1955 a         2234 c         2519 g         1923 t
ORIGIN      
        1 gccgagggat ctggctgcga tccgggagac gctgactcac gtgggggctg tccgcagaga
       61 gctcgatcct tgcaactcga cgatcttgaa gtcgctgcaa accaagattg ccgaattgcc
      121 tgaagtggtt ttgctcatcg aaagcgctat cgtcgatgcg ccgcccctat cggtcaagga
      181 gggcggtttt attcgcgccg gttttgatgc ggagcttgat gaggttcgcg gtatgcgcgc
      241 tcatgccaag gaatggatag ccgctttcga ggccggagag cgacggcgca cgggaatcca
      301 atccctaaag gtgagataca accgggtctt cggttattac attgaagtga ccaggtcgca
      361 tctaaaagcg gtgccggagg actacattcg caaacagacg ctcgccaacg gcgagcgtta
      421 tatcaccccg gagctgaagg agtatgaggc caagattctc aattcccagg aactgatcga
      481 gaagttagag attgccttgc tggcccgggt gcgcgaacaa gtcgccgttc attttatcgc
      541 tttgaaggag atgagcgtcg ctctcgcgat gctggacgtg ctgttgtcat ttgcggaagt
      601 cgccgagtcg caacatttta cccggccacg ggtcaataac ggcttcgcgc tgatcatccg
      661 agaggggcgt cacccggtgg tggaagccac gttaggccgc ggcggcttcg ttcccaacga
      721 ctccattctc gacccggcat cgaatcagat cctgctgctc acaggaccca atatggcagg
      781 caagtcgact tatatgcggc aggtcgcatt gattgtgatt cttgctcaga tgggcagttt
      841 tgtgcctgca accgaggctc gtattggcat agcggaccgt atctttaccc gcattggagc
      901 caccgactca ctggcgcgcg gcgagtcgac tttcatggtc gagatgaagg agaccgcgaa
      961 tatcctgcag catgcaaccg agcgcagtct catcctgctg gatgaagtcg gccggggcac
     1021 cagcacgttt gatgggatat ccatcgcctg ggcggtggcg gagcgtcttc atgacatgcc
     1081 ggggcgaccg cgaactcttt ttgcgacgca ttatcatgag ctcaccgatc tggcgcgaac
     1141 caaggaaagg atcagaaact ttaacttttc ggtcaaagaa tggcaggggg acgtcatttt
     1201 tctgcgcaat ctagtcgaag gcgccgcgag tcacagctat ggaattcacg tgggacggct
     1261 ggccggcttg ccgccggcgg tcatcgaacg ggccaagcaa attttgcaaa acctagagag
     1321 cggcgaagcc gagggaaaac gcgcggatct ggaaaagaga gaggttgacg tggacgaacc
     1381 cgtgcagatg gcgttgttcg gatccgttga aaggaaattg ggcgaggagc taaaaaaact
     1441 cgacgtgtcc cgtctatcgc ccatcgaagc gctgaacttg cttcaccaat ggtcggagga
     1501 agcgaaaaag taggcacgag ctgtagagta tcttatggct aagagacttc tactcggcat
     1561 attcggactt ctgcacttgg ggctatgggt accccagggc tgcgcagggg ggacggcagc
     1621 tccgcatcgc cctgcccagg ttcagaagaa agctccggcg acaagtccga gcgccagtcc
     1681 ggccaataat gaggcgcttg aggttgatcg cgtggcgcag tcgacgaagg tttcggcttc
     1741 gccggacaga tcgcgtctgt tctcggtgcg cgtgtcatcg tcccagacct acagtagggt
     1801 cacgatggac ctctcccacg aggtgcgcta tgaaacccat cgcctcaagg aggatccgtc
     1861 taaaggattg ccaccgcgga tctatgtcga cctgttcagc accaagctgg cgatggatgc
     1921 caggaatccg attgcggccg atgatggtgt gttgcgccag ttgcgcgtgg gccaattcac
     1981 tgccgatgtc gtacgcatcg tgatcgactt gacgacctcg ggggagcata acgccttcct
     2041 gttggctgat ccttatcgat tggtgattga tatccaagga cagaaaaatt ccgaagcggt
     2101 cgctgcggcc gacaagagca agaagaaaac ggcgaccttg ccggaaagag caaagccgct
     2161 tccggttccg gccatccgca aggttgtttt ggatccggga cacggcggca aggatcctgg
     2221 agccattggt gtcgggggga tcgcagaaaa agacattgtt ctggctgtag cgaaaaagtt
     2281 ggaacggaaa ctcaagcaag aaatgggagt ggaggtggtt ctgacgcgaa gggatgaccg
     2341 attcattccc ctggaagatc ggacggcgat cgccaacgcc gaaggcgctg atcttttcgt
     2401 ctctttgcat atgaatgcga gcccaaatgg tgaggcgagg ggtctggaga cctattacct
     2461 ggacaacacc aatgacgagg gttcgctgag gcttgcggcg cgagaaaacg gcacctcgcg
     2521 caggaatgtt tcggatttgc agtttatctt gagcgatatg actcaaaaca tgaagttgga
     2581 ggactccatt accctcgccc atcacttaca gcattcgctg gttgacggca tgtcaaccag
     2641 gcttaccgac gtcaaggacc tcggggtcaa gaaggcgctc ttctacgtac tggtgggagc
     2701 gcgtatgccc agcgttttgg tagagatgtt tttcatcacc aacaagaccg aagggcgcgc
     2761 catgagtcaa gagagctacc agaacgccgt ggttgacgca ttgtacgatg ggatcctgaa
     2821 gtacaaggaa agcaacgtgg caagcaagac gttataaagg gacctttggc gtggacgcaa
     2881 tcgcactttc ccgctccctc ttagacgaaa cccaggcgga gtcggacctc cgacgcgcgg
     2941 cgcggagcta tgtcaaaaag ggccgggagc tgctcctgga aaaacaccgg gcaggtgccg
     3001 ccggagtcga ggtggtatcg gtctactcga cgatgatgga ccaattgatc cgctatcttt
     3061 tcagtaaagt aagcgacgac ttcattcgca gatatcccag tgaaaatccc cgctgcaccg
     3121 tcgtcgccca gggcggatac ggcaggggag aactcaatcc ttattccgat atcgatctac
     3181 tgttccttta ctcttggaaa atttcgcctt acgtcgaggc ggtgacggaa aggctgctct
     3241 acacgctgtg ggacgccggg ctccaggtgg gtcacgccac gcgcaccatc aaagaatcca
     3301 ttgcgttgtc cgcctcggat atgaaagtga agacggcgct gctggacgcg cgctacctct
     3361 gcggtcatta cgagctctat caggagtttg acaaggcagt ggagaaccgg ttggtcaaca
     3421 agcgggtcag ccgattcgtc caggaaaaac tgacggaaag tcgcctgcgc catgaaagct
     3481 acggcggatc ggtctatctt ttggagcccg atgtaaagga cggcgagggc ggtcttcgcg
     3541 atatccatac cgcccgatgg atcggccggg taaaggcagg cgcgaaggat ctcgatgggc
     3601 tggccctaaa gggaatcgtc aatcccgacg atgtcgccaa actcaaagcg tcccaggact
     3661 ttttgttgcg cgtgcgcaac gagctgcatt tttcgacggg caaacaccaa gaccagttga
     3721 ccttcgaaga gcaacaaaag gtgagtcaag cactaggctt cgaaggcgag gggactttga
     3781 aggccgttga agttttcatg cgcaattatt atctccacgc cgcgcagata agccgcctcg
     3841 cttccttgat cattcaccgc ctcaccgatg ccccgcgccc gcttgtgggc ggactataca
     3901 gtttcgccag gaccctgcgc gagggcattt gcgtttcgca cggacagctg ttcgtcacaa
     3961 agccaagtat tcttaagtcc gagccgggca atctcattga tgtcttcgcc gagatgcaaa
     4021 agcaccgctg cgagctgagt cacgaaacgc gggagctctt gcgcgagcac atcgggttga
     4081 tcgatgatcg atttcgtcgt tcggcagcgg ccaacatgcc gtttttcaag attctcaagt
     4141 ggaaggagag ggtttacgaa acactcttgg aaatgtaccg ttgcgggatt ctcggagcgt
     4201 ttattcccga gttcggcagg ctgctttgta tggtcctcca tgacgcctac catatttaca
     4261 cagtggacca gcattccctc aaattgatcc aagagatcga gaggcttaaa gccggagcct
     4321 acaaagaggg gatgccgctg ctcacccaac tggcccgcga cgctgataag atcgagttac
     4381 tttatctcgg cctgatgttt cacgatatcg gcaaggggtt tggcggggga cactcggaga
     4441 tcggcgcccg tttggtgtcg cccattgcgc ggcgcatgcg tctcaacgcc gacgacgggt
     4501 ctctggtgga atttctcgtg cgccatcacc tgttgatgac gcacacggcg tttcggcgtg
     4561 atttggaaga cgagaaaacg atctttgact tcgcaaaaac aatgggcaat gtcaacaacc
     4621 tcaagatgct ctacctcctg acatttgccg acgtcaaagc ggtcggccct gatgtctgga
     4681 atccgtggaa agcgtcgctg ctgggagagc tctatgtcaa gacgttaaac gtcctggagg
     4741 aaatggaaaa aggcgaattc caacgtgaag atgtgcgggc ggggttgcgc agaatccagg
     4801 cccgggtcca gcgcctgctg gccaaggacc acccggccga tagggtggac cattttctgg
     4861 agtcgatgcc ggaaaggtat tttctttcca ccgcagagag cgatattccg gctcatttcg
     4921 agttgatgga aaagtttcgg gggaagggag cggttaccgc cgtcgagcac tttcccgaaa
     4981 gggattgcag ttccatcgtg atctgtaccc aagatcgccc ggggctgttt gcttcgatta
     5041 ccgggacgtt gacggccttg aatctggaca ttctgaacgc caggattttt acttcgagcg
     5101 atggccgcat cctggatgtt tttcgggtct cgcaccgagg ccgttccgag ctggtcatgg
     5161 cggaacaaaa gtggagtaaa tttcgctcga ctctggacga cgttttggag ggtcggttgg
     5221 atgttgcccg gctggtggaa aactcgcggc gcgccccatt cttgcagcga catgtaccta
     5281 aggtgcccac ggttgttcag gttgatattg aagcgtcgga caatttcacg attgtcgaag
     5341 tattcaccga ggatcggatc ggggtcctgt tcaagataac ttatgcgctg catcaactcg
     5401 gcttgtctat ccacgtcgcc aagatatcga ccaacgtgga tcaggtggcc gatattttct
     5461 acgttgccga cgaaaatgga cgaaagattg aagagccggg tcgactggaa gagattcggc
     5521 tatcccttta cgaaagtcta gcgccacaag atgaaagagt cgctcagtcc tcacattgat
     5581 tcctttctta cgatgatcgc ggtggagaag gggctggcca aaaatacggt cgaggcctac
     5641 agtcgtgatc tggttcgact gtcggagttt ttagtggccc agagcgttgc ctcctgggaa
     5701 gagacagaga ccttgcatct ccgttcctat ttatcgtctc tccgccgcaa cggtttgagc
     5761 ccgcgttcca tagcccggca catcgttacg gtacggcgat tctatggttt cttggaaacc
     5821 gaaggagtca tcaatgagaa tccggtgccg ccattccatt tggcggccgg cgggagaaaa
     5881 ttgccgcaaa ctctctccgg ggacgatgtg cgaaagttgc tggatcagcc gaatccccag
     5941 gaactccttg gtgtccgtga tcaggcgatg cttgaactgc tgtatgccac cgggctgcgg
     6001 gtttcggaac tgatctcttt gcagatgcag cagatcaatt ttcagggaaa ttttctgacc
     6061 gtgaagggca aaggctcgaa ggtgagagcc gttcctttcg gaaaatgggc gcggcaaaag
     6121 ttactcgact atgtcaatgg tgcaagacgg cgactggcca agggtagagc cagcgttttt
     6181 gtatttatta accgctcggg ccggcctttg tcgcggcagg gcttctggaa gcttattcga
     6241 gggcatgcgc gggccgccgg cgtcgaaaag cgcgtaacac ctcacaccct gcgccattcg
     6301 tttgctaccc atcttctcga gggaggcgcc gatctccgct ccgtgcaatc aatgctaggt
     6361 catgcggata tctcgacgac ccaaatctac acccacgtga acggcgccag actcaaggag
     6421 gttcaccggc gccatcatcc aagagagcgg ggcgaccgga aacaagactt ggaatgatgg
     6481 agtactggag ttgtggagta ttggcagaaa atgcacgcta gaactaccca tcactcgact
     6541 actccatcac tccgggtttt tcttcaccag cttgagtttt tccagggctt gccgtaatca
     6601 tgttgaaata gcgattaagc gagaaggttt tggtagacgc catccaacaa atcgccctgt
     6661 gggctcttcc cgtcctggtg gcgattatct ttcacgaagt atctcatggc tgggtcgctt
     6721 atcggttagg ggatcctacc gccgccagaa tgggtcggct gactctcaat ccgatagctc
     6781 acattgaccc gatcggaacc atcttgttac cggcgctgtt gattatttcc aacgctccat
     6841 tcttgttcgg ttatgcgaaa cccgttccgg ttaattttca caatctgagg aatccgaagc
     6901 gggacatggt ctgggtcgca ttggccggcc cggtcacgaa tcttgtgttg gcggctggtt
     6961 gtatcgtggt tttgaagatt ggcctgcccc tcattgcggc gggagcgatt tattcttctt
     7021 cgtccggcgt ggtgaacttt ctcgcgcccg tggcactgat ggccaaaaat ggagtgttga
     7081 tcaatatcgt cttagcggtt tttaacgcat tgccgcttcc gccattggac gggggcagag
     7141 tggcggtggg attacttccc gaaccctact cttcgatgtt ggcgcgggct gaaccctatg
     7201 gttttttgat tttaatcata ttgctgatga cccacgcgct ggacaaaatt atcgggccac
     7261 cgactgagtt tttaattaac ttttatttca agttccttta gacttggtaa ttatggatac
     7321 cattgttagc ggtatgaggc ccacgggacg gctccatttg ggtcatcttc atggggcgct
     7381 caagaattgg ctaagactcc aggaaaatta tcgctgctac tttttcgtcg ccgattggca
     7441 cgcgctgacg acggactacg cctcgccaca ggggatcaag ccgagcacga gcgagatggt
     7501 gacagactgg ctgagcgttg gcctggatcc ccgccgctcg actatttttc ggcaatcgca
     7561 ggtgaaagag catgcggagc tccacttgct tttctcaatg ataacaccgg ttccatggtt
     7621 ggagcgcaat cccacgtata aagaccagct caaggaattg gtggaaaagg atctgtcgac
     7681 ctacggcttt ctcggctacc cggttttgca agccgcggac attactatct ataaagccaa
     7741 caaagtcccg gtgggagtcg atcaagcgcc ccacgttgaa ttgacccgcg agattgtgcg
     7801 gcgcttcaat cagatttacc gtcccatttt cccagagccc gaggttctcc tgaccgaggc
     7861 gcagaaggtg ccgggcttgg acgggcgaaa gatgagcaag agctacaaca acgccgtgtt
     7921 tttgtccgac acaccgaacg aaatcgacca aaaattgagc cgcatgatga ccgacccggc
     7981 gcgcgtcagg cggaccgatc ccggtgaacc ggaaaaatgc ccggcgtttc aactgcacaa
     8041 gatttattgc acccctgaag agatcgcaga agtttcaacg ggctgccgca ccgcgggcat
     8101 cggttgcctg gattgcaaga aggtgatgat caagcatgtg atcgaagatc tagcgccctt
     8161 tcgcgagcaa agggcgcgtt atgagaaaga ccctgagcag gtcgaagcgg tgctgacaac
     8221 gggcaatgcg atcgctcatg aaaaggctgc cgaaaccatg gttgaagtgc gggaagccgt
     8281 aggactgtga ccacccgagt acaactcgaa attttcgaag gaccgttgga tctgctgctt
     8341 catctgatta aaaagaatga ggtcagcatc accgatattc ccattgcgac catcaccgaa
     8401 caataccttg cgacgttgga ggttatgcag gctttcaatc tcgacgtcgc cggggaattc
     8461 ctggtcatgg cggctaccct cattcacatt aagtcgcgca tgctcctgcc tatgaccgac
     8521 gacgaagacg atgaagagga ggggacagat ccacggcagg agttggtgcg gcggctgctc
     8581 gaataccagc gctttaagga tgccgcggat caactggagc gaagggaact g
//
LOCUS       DASSWZ010000049         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_213704, whole genome shotgun sequence.
ACCESSION   DASSWZ010000049 DASSWZ010000000
VERSION     DASSWZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213704"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1087)
                     /locus_tag="VFN58_01175"
     CDS             complement(29..1087)
                     /locus_tag="VFN58_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HET8916628.1"
                     /translation="MAAPKTDETLVHVLWINAGLSCDGDSVALTAATQPSIEEIVLGA
                     LPGLPKIAVHWPLIDFECGPGGLGEPGADTFIKWFYKAERGEIDPFVLVVEGSIPNES
                     IKKEGYWCGFGNNFETGQPMTTSEWLDRLAPKALAVMAVGTCATYGGIHAMAGNPTGA
                     MGVPDYLGWSWKSKAGIPIVCVPGCPIHPDNLSETILYLLYQATGQAPMIPLDDQLRP
                     TWLFGQTVHEGCDRAGYYEQGDFATEYGSPKCIVKLGCWGPVVKCNVPKRGWINGIGG
                     CPNVGGICIGCTMPGFPDKFMPFMDEPPGSKVSAGISGVYGKLVRGLRGITERTVDKE
                     PKWRHRGNELSTGYQKQW"
     gene            complement(1352..1804)
                     /locus_tag="VFN58_01180"
     CDS             complement(1352..1804)
                     /locus_tag="VFN58_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916629.1"
                     /translation="MFIETYYDDRATVPGSTSEPMIPYPELSGEELEVWRKYLPMRRQ
                     IRNLDSRLNRDTIPGPVITELEKARKAPGLFDRVEIWSRADDPMAVGIIGEEKTRYFA
                     IARWGDAELTFEQVKKRLRAEKWMLWLASTVGILVFLAATLVAIANGG"
BASE COUNT          532 a          683 c          579 g          539 t
ORIGIN      
        1 gcccaaccta aacaacgcgg ttgctgcctc accattgctt ctgatatccc gttgacaatt
       61 cattaccgcg atgccgccac ttcggttctt tatcgacagt cctttcagta atgccgcgca
      121 aacctcgaac caactttcca taaactccgc tgatccccgc ggaaaccttg ctccctggag
      181 gttcgtccat aaatggcatg aacttgtccg ggaaccccgg catcgtacag ccgatgcaaa
      241 ttcctcccac gtttggacag ccgccgatgc cgttgatcca gccgcgtttg ggcacgttgc
      301 atttgaccac cggtccccag cagccgagct tgacgatgca cttgggcgag ccgtattcgg
      361 tggcaaaatc gccctgctcg taatatccgg ctcggtcaca gccttcgtgc accgtttgac
      421 cgaaaagcca tgtgggacgg agctgatcgt ccaggggaat catcggcgcc tggcctgtcg
      481 cctgatagag caaataaaga atcgtctccg ataaattgtc gggatggatc ggacagccgg
      541 gcacgcaaac gatcgggatg ccggctttcg acttccaact ccacccgaga taatcgggca
      601 cgcccatggc tccggtagga ttgccagcca ttgcatggat gccgccgtac gtcgcacagg
      661 ttcctacggc catgacggca agcgctttgg gagccaaacg gtcaagccat tcactcgtgg
      721 tcatgggctg cccggtctca aagttatttc cgaagccgca ccagtatcct tcctttttga
      781 tggactcgtt ggggatagag ccttcaacga ccagcacgaa aggatcgatc tcgccccgtt
      841 cggccttgta aaaccatttg atgaacgtgt cggctccggg ctcgcccaag ccgccggggc
      901 cgcactcgaa gtcgatcagc ggccagtgaa ccgcaatctt cggcaagccc ggcaaggcgc
      961 cgagcacaat ctcctcgatg ctcggctgcg tggcggcggt gagcgccacc gaatccccat
     1021 cacaactcaa gccggcattg atccacaaca catgaaccag agtctcatct gttttcggcg
     1081 cagccatttg tgcatgaacc tccttttttg cgttagcgat cggctctcgt cagtcgactt
     1141 ctaaaagcaa tgcttcggcg aaaatgggct tacctatcca atttaaagtg actaatggag
     1201 gaggcgctcg cccgcaatac ggagtacgcc cgtttctttt atgagggatt agccgagccg
     1261 ctcctagggg aatcccctag gagagaagga ctggagcact gagggatgaa gtagtggagg
     1321 tgatttctct tatcgcgacg gcaggggtcc tctatccgcc attcgcaatg gcaaccaacg
     1381 tggcggcgag aaaaaccaag atccccactg ttgaagccaa ccaaagcatc catttctcgg
     1441 cccgcagtct ctttttcacc tgctcgaacg tgagttctgc atccccccaa cgtgcgatag
     1501 caaagtagcg tgttttttct tccccgatga ttccgactgc catgggatcg tccgcgcggc
     1561 tccagatctc tacacggtca aataagcccg gcgcctttct tgccttctct agctccgtaa
     1621 tcacggggcc aggaatagta tccctattga gccgtgagtc gaggtttcgt atctgtcttc
     1681 tcatcggcaa gtattttcgc catacctcca gttcctcccc agacaattca gggtagggaa
     1741 tcattggctc gcttgtgctg ccaggaaccg tagccctatc atcataataa gtctctataa
     1801 acataaagcc tccttaatat gctaaaaagt tctgcatcgg atgggtggcc tctgaaaaac
     1861 aaaagccagt acactcttgc ggcacaaccg caaaagcgac tggctttgat gactcattta
     1921 tcttctgccg aggaacacac ttcgttcctc tcaaggttca ctaacctaag ctaaccctat
     1981 cggagatgag gaagctctgt ccaaggatga aaagctatca aggtgtatgg gctgcgacgc
     2041 tttgacagca tgcttttagc actgggcaac gtgactgtca acaacaagaa tgacatgtaa
     2101 cttctgatca tctggtttcc tgatgcggcc gcacatatca gggaaatcta gcggtgtgtt
     2161 tgtggagcgt caagacggta tcaatgcaag acaccgaagc gagtgagcgc aggctttagc
     2221 gagcaccacg agcgaaggcc ccttcctcca ccaagcgaag cagtatattt agccacgccg
     2281 cttatctatc cagtccttgc atagctccac tccgtgttgc tctgcttgct ctt
//
LOCUS       DASSWZ010000050         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1076966, whole genome shotgun sequence.
ACCESSION   DASSWZ010000050 DASSWZ010000000
VERSION     DASSWZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076966"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..743)
                     /locus_tag="VFN58_01185"
     CDS             complement(207..743)
                     /locus_tag="VFN58_01185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET8916630.1"
                     /translation="MKGLSPLMITIRAETSKDIAAIRHVLEMAFGQASEARLVEALRQ
                     VDALITSLVAVEKEQIVGHIAFSAVFIESLHATAKVVALAPLAVLPTHQRRGIGSELV
                     RRGLTQCRRAGHRVVLVLGEPAFYQRFGFSKASLHGICCPFDVPDDAFMAAELFPGAL
                     AGQSGSVKYRPEFDLVSG"
     gene            820..2586
                     /locus_tag="VFN58_01190"
     CDS             820..2586
                     /locus_tag="VFN58_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HET8916631.1"
                     /translation="MMRTKSELRRLIQGAMGEIKADLIVTGGKLINVYSGEILDGTEI
                     AVIDGRICYVGPSAAYARGAATKILDAGGLYISPGFIDGHTHIGHYCRPYEYLQAYLP
                     HGTTALMASCDELATVFGYRGVELFLDEVAAHPLRVLTLISMVAPQDPLLCQTATITN
                     DEIEAALQDPRILGFGEIVSWLRLTQLDEEILERLEMAWRSGKIIHGHTAGAKDQKLC
                     AVAAVGISSCHEPIRVEDALARLRLGYWTMLREGSLRQDLEATLTPLIARGIDTQRLI
                     LVTDSMSPDDVEELGHMDHVLRRAVGLGLSPLRAIQAVTLHPATYSGVEQEVGGIAPG
                     RFADMVLLEDLESFRVQATLIGGKMVAEEGRSLVQTAPLSVPHEMMHSLRLRPNISEK
                     QFRVHCQAGNAKIRVIELLNQNITAERVMSLDARKGYIEADVEQDLLKIAMFDRHEQI
                     GKITMGFLKGFGAKIGAVGTTTDLDENTLLIVGSQDADMTLCANVLIESGGGMAVVRN
                     GQVVDKIEFPVGGIFGLNPWRQVGQQLRRIHRYLREQGSPFERPMYALCFLPFVTLPS
                     LRITARGLVNAKERKIVPLLVD"
     gene            complement(2589..2993)
                     /locus_tag="VFN58_01195"
     CDS             complement(2589..2993)
                     /locus_tag="VFN58_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET8916632.1"
                     /translation="MAEPNKLGESGAYLGPGTKINGKLQFDGPATIEGEIEGEIIAHA
                     NVTIGQQATLKGKISAVSVVVQGKVMAEVQAEKKLEIQSPGSIVGDVNTQTLVIGDGA
                     VLEGHVSMRKEGRVLPLRQDVKPAAAKEGTSS"
     gene            3306..>4301
                     /locus_tag="VFN58_01200"
     CDS             3306..>4301
                     /locus_tag="VFN58_01200"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8916633.1"
                     /translation="MELTDLRDYLEILEEYGELQRVQSEVDWNLEMGAITRRCYDLGA
                     PAALFENVRGYPKGYRALGAPLGASRHPKHSLFARTALALRLPPHASAKEIMQTYLER
                     KEKLIPPVRVNTGPCKENIILGDDVDVLKFPIPLIHGGDGGRYIGTWHTVITKDPDSS
                     WVNWGMYRLMVHDRNTLGCLFPLQQHIGQMYQKYEAMNRPMPVAVAIGGQPVIPIVSC
                     VQLPPYVNEVEVAGALQAGPIPLVKCETSDLEVPATAEIILEGEVLPHERRVEGPFGE
                     YMGYEAGKSSPKPILKIHAITHRRDPILPFSNMGMPVHEGQTATALIKGAEIYGEL"
BASE COUNT         1003 a         1080 c         1221 g          997 t
ORIGIN      
        1 gccgtcgaga tcgaaaatcg acttgggcga cttcggatgg aaccaagtct ggggcactag
       61 gtgagcgtga caatcgatga tcatgagcaa ttacctccaa aatcgacatt aggggtcggg
      121 gagttgaatg tcaaatttcc aatttggcat ctgcgtcacg ccgttagaca tgtaagggat
      181 cagttagcgt tagattgggt tttaattcac ccgctcacca agtcgaattc agggcgatac
      241 ttgaccgatc cactttgtcc tgccagcgct cccggaaaca attctgcagc catgaacgcg
      301 tcatctggaa cgtcgaaggg acagcagatg ccgtgtagtg aagccttgct gaaaccgaag
      361 cgctggtaga atgcgggttc gccgagtaca agaacaacac gatggccggc gcgccggcac
      421 tgcgtcaaac cgcgtctcac caattcgctg ccgatgcccc gccgttggtg ggttggtagc
      481 acggcgagcg gagcgagcgc gacgaccttt gcggtagcgt gtagtgactc aataaacacg
      541 gcactgaaag cgatatgccc gacgatttgc tctttctcga ctgcgactaa cgatgtgatc
      601 aaggcatcga cttgacgaag ggcttcaacg agccgtgcct cgctggcctg gccgaaggcc
      661 atctcgagga catgccggat agcggcgata tccttggaag tttctgcgcg gattgtgatc
      721 atcaggggag agaggccttt cacgggcaag gcgattctat tgtgattttc atacctccac
      781 aactttagta accaagacca tgtgtgttaa aaacggaaca tgatgcgaac gaagagcgag
      841 ttgcggcgcc tgatccaagg tgcaatggga gaaataaaag ccgaccttat cgtaaccggc
      901 gggaaactta tcaatgtcta ttccggggaa atcctcgatg ggacggaaat tgcggtaatt
      961 gacgggagaa tctgttacgt cggtccgagc gccgcttacg cgcgtggcgc ggcgacgaaa
     1021 atcttggacg cgggtgggct ctatatttct cctggtttta tcgacggaca cacccacatc
     1081 ggccactact gccggcccta cgagtatctt caggcatatc ttccgcacgg gacgacggcg
     1141 cttatggcgt cttgcgacga attggcgacg gtttttggct atcgtggtgt ggagcttttt
     1201 ctcgatgaag tggcagcgca tccgctgcgg gtgttaacgc tgatttcaat ggtagcgcca
     1261 caggatccgc tgctctgcca aacggcgacg atcaccaacg acgaaatcga agcagcactt
     1321 caagacccgc gcattctcgg gttcggcgag atcgtttcgt ggctacgctt aactcagctc
     1381 gacgaggaga ttctcgaacg gctcgaaatg gcatggcgca gcggtaaaat cattcacggc
     1441 cacacggccg gtgccaaaga ccagaaacta tgcgcggtgg ctgccgttgg aatctcttcg
     1501 tgccacgagc cgattcgcgt ggaggatgct ctggcaaggc tgcgattggg gtactggact
     1561 atgctccgtg agggttcgtt acgccaggat ctagaggcga ctctcacgcc gctcattgca
     1621 cgaggtatcg atacccagcg tttgattctc gtgaccgaca gcatgtcacc ggacgacgtt
     1681 gaggagcttg gccatatgga ccatgtcctt cgacgagcgg ttggtttagg gctttccccg
     1741 cttcgcgcga tccaagccgt gaccttgcat ccggcgactt actccggcgt ggagcaagaa
     1801 gtgggtggaa tcgcgccggg ccggtttgcg gatatggtgt tgctggagga tttggagagt
     1861 ttccgagttc aagcaacact gatcggaggc aaaatggttg cggaagaagg gcggtccttg
     1921 gttcaaactg ctccgctttc ggttccgcat gagatgatgc acagtcttcg attgcggcca
     1981 aatatctcgg agaagcaatt cagagtgcac tgtcaggcgg gaaacgccaa gatccgagtg
     2041 atcgagttac tcaatcaaaa cattactgcg gagagagtca tgagcctaga cgcgcgaaaa
     2101 ggttatatcg aagccgatgt tgaacaggat ttactgaaaa tcgcgatgtt tgaccggcac
     2161 gagcagatcg gcaaaatcac catgggtttt ctcaagggat ttggtgccaa aatcggcgcg
     2221 gttgggacca ccaccgatct cgatgaaaat acgctgctga ttgtaggaag ccaggacgca
     2281 gatatgacgt tatgcgccaa tgttttgatc gaatcgggtg gaggaatggc ggtagtccgc
     2341 aatgggcaag tggtagataa aattgagttc ccagtcggcg ggatttttgg cctaaaccct
     2401 tggcgccagg tgggccaaca gcttagacgt attcatcgct acctccggga gcaaggctcg
     2461 ccgttcgagc gtcccatgta cgctctttgt tttctccctt ttgtaaccct gccttcgctg
     2521 aggatcacgg cgcgcggttt ggtcaacgcg aaggagcgca aaatcgtgcc cctccttgtg
     2581 gattgaatct atgaagaagt gccttccttg gcagccgctg gcttaacatc ttggcgcagc
     2641 ggaagaactc tgccttcttt cctcattgaa acatgacctt cgaggacggc gccatcgcca
     2701 atgacgagcg tttgggtatt cacgtcgccg acaatcgagc ctggcgattg aatctccaac
     2761 tttttttcgg cctgaacctc ggccatgact ttgccctgga cgaccacgga aacggcgctg
     2821 atttttccct tcagcgttgc ctgttgaccg atcgttacgt tggcgtgcgc gatgatctcg
     2881 ccttcaattt ctccttcgat ggtcgctggc ccgtcaaact gtaatttccc gttgatcttt
     2941 gtgccgggcc caagataggc tcctgattct cccagtttgt ttggctctgc cattgtgcct
     3001 cctttcttcg agccacaaag tataaaacac aaggtaattt ttgtccacaa ttactaaaag
     3061 ctttatagca atgtagcgat atggcgaccg agagaacgat tcctagaaag cgcgggcacg
     3121 caagtataaa ggtcgcgttg cgcgaactta gctactctta aaaaagtcgg agcagcgggt
     3181 aacagtctaa gcgcttttgc atatctgaca ggctgtctgc tataaaattg ttaaagctta
     3241 aacaattagt ttccctgggt gatagccccg ggcaactcga atgccaacct gacaggggac
     3301 attaaatgga attaaccgac ctcagagatt atttggaaat cttagaagag tatggggagc
     3361 tccagcgggt tcagagcgaa gtcgattgga atctcgagat gggggcaatc acgcgccgat
     3421 gctacgatct gggggctccg gctgcgcttt tcgagaacgt aagaggttat ccgaaagggt
     3481 accgcgctct cggcgcaccg ctcggagcca gccggcatcc gaagcattct ttgtttgcgc
     3541 gtaccgccct cgcgctgagg ttgcctcccc acgcgtcagc caaagaaatc atgcagacct
     3601 atctagagcg aaaagaaaaa ctcattccgc ccgttagagt taacactggg ccgtgcaaag
     3661 agaacattat tttgggtgac gacgtcgatg ttcttaaatt cccgatcccg ttgattcacg
     3721 gcggtgatgg cgggcgttat atcggcactt ggcataccgt catcaccaag gatccggata
     3781 gttcgtgggt taactggggt atgtatcgtt tgatggtcca cgatcgaaac acgctcggtt
     3841 gtctctttcc gttgcagcaa catatcggtc agatgtacca gaagtacgaa gcgatgaacc
     3901 ggcccatgcc cgtggcggtt gcaatcggcg ggcagccagt aatacccatc gtaagttgcg
     3961 tacaacttcc tccatacgtc aatgaagttg aagtagccgg tgcgctacaa gcagggccga
     4021 ttccgctggt gaaatgtgag acctccgatt tggaagttcc agccacggct gagattattc
     4081 ttgaagggga agtcctgcct catgagcgaa gagtggaagg accgttcggc gaatacatgg
     4141 gctacgaggc aggtaagtct tcaccgaagc cgattttgaa aatacacgcg attactcatc
     4201 ggagggatcc gattctgccc ttctcgaata tggggatgcc agtacatgag ggacagaccg
     4261 caacggcttt gatcaaaggc gcagagatct acggggaatt g
//
LOCUS       DASSWZ010000051         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1190328, whole genome shotgun sequence.
ACCESSION   DASSWZ010000051 DASSWZ010000000
VERSION     DASSWZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190328"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VFN58_01205"
     CDS             complement(<1..1203)
                     /locus_tag="VFN58_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8916634.1"
                     /translation="MRFSPTPWLVATLAFVILGFTRGLHSSFGVFYVALLDTFGWSRG
                     ATAGVFSMVLTVDAILSPVVGYLLDRFGTKKIVCSGCFILALGLLLSSTIGSLWQLYI
                     FFGLISAVGITFTGMVPHVFLISEWFVANRASAIGFVYAGTGVGILLLAPLSEWLLSS
                     WGWPRAFQTFSGIVLLLLLPLVWLFYQHGPFGEKLRSKKEAKSDDHRWTAKLALRSWQ
                     FWLLFFARICAACGTTVIVTHQVAHVVDVGYSKLLAATIFGLAGITSSFGRVVFGFIA
                     DLLTKQAAYTLNILMTLVGVGALMILRDPSQTWLLYVYVIFFGIGFGSRAVIFSALTA
                     DIFSGKGFGSILGYSTVAVGVGGALGSWLGGAFYDWTGSYLVSFALSALVLGCSDVCI
                     WLASMPSVA"
     gene            1298..2146
                     /locus_tag="VFN58_01210"
     CDS             1298..2146
                     /locus_tag="VFN58_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916635.1"
                     /translation="MTKQNLICGLGLLLIIAGCATLESGGEFTSGRRALMSGDSAVAL
                     TYFEPIAKRDPNYIAPFSSFRESIWTYLGRAQYQSGKLVEAKSSLERALSQIPNDSVA
                     KLYLALTNLRLQTTEKATNPFTLQDISFALRERVEPKRVAALVRERGVAFDLTTESES
                     QLRKAGADDFLIDEIKKIRADALKQRKTSESQLGQSTKELAAALTAIRDQLDYLNSTR
                     QGIFWDPNKEIRSQIQTGLSLLSAPQPDWQKALSTGEWIGQRIEEEIDLARRDEAEEL
                     RRQDRR"
     gene            complement(2171..3751)
                     /locus_tag="VFN58_01215"
     CDS             complement(2171..3751)
                     /locus_tag="VFN58_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HET8916636.1"
                     /translation="MADIFLSYANEDREAARAVAGLLESAGCTVWWDRRIPAGRTWRS
                     MIEEALRDMRCMVVLWSSHSVESDWVKEEAEEARAVGKLIPVLIEAVKPPVGFRSIQA
                     ADLIDWDGSRDFLGARQLIADLESLMGKPPPQKLSETDGEPRKSPPIPQPIEEARVVN
                     RSVSNNEDSTLAGSPSVQRSGSTTIESDRKSDTRWKIAVVGALIVVAVLGSLLWFAKK
                     DRRTSGPEVVAVKPPPPVPAPNLVSLGLSGDRQEIAANETLNVILKGQYSDGTEKNLN
                     RGVKWLSSEPGVATIDDQGQVTARQAGETKITARYGDLVSPAWTLAVRAEKPAVTVTA
                     PAEKPAVKTPVITRLVALAIFTERREVRTQERLSLRVKARYSDGNEKGLSSGVEWRSS
                     DTAIASVDSRGELLALRAGKITVIARWGGIESSAVNILVRESPAKPPPEPPTYKPAVE
                     PPQVTPAKPPVQTINIASYINRAKSHRVQGNYAAALAELEKARAISPGSQEVFDEIEA
                     TRRACNAEQRLGREGLTC"
     gene            4023..>4796
                     /locus_tag="VFN58_01220"
     CDS             4023..>4796
                     /locus_tag="VFN58_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HET8916637.1"
                     /translation="MVIRELKDGSSIVSTQEDHAELSAQFAAHWGNKDFARLRPYKTM
                     VFATTYHDSGYREWEGNPPMNVAKGRPYAHREEIPSFEPVELRAYARNVEWLSSQDSY
                     AGLLVSMHRTGLWQNRYQTFTSPKGRLRERSPEVKAAMQQLEAKQQEEKKSLGLGNPT
                     FENDLWFNYRALQIYDLLSLYFCCDGYSDDDHFKEERLAPIRLSYDSQAEVELRIVPA
                     GVRSVRMEPYPFDIGPLTVSARARIIPPGTYASEEACLEA"
BASE COUNT         1166 a         1375 c         1290 g          965 t
ORIGIN      
        1 ggcaaccgat ggcatcgaag ccagccatat acagacatcc gagcagccca agaccagcgc
       61 cgaaagcgca aaggagacca gatagctgcc ggtccaatca tagaacgcgc ctcccaacca
      121 ggagcccaac gctccaccga cgccgacagc gaccgtcgaa tagccgagaa tcgatccaaa
      181 tcccttgccg gaaaagatat cggcggtgag cgcggagaaa atgactgcgc gcgagccgaa
      241 gccaatgcca aaaaaaatga cgtaaacgta aagtagccat gtttggctcg gatcacggag
      301 gatcatcaaa gcaccgacac ccaccagagt catcaagatg ttcagcgtat aggccgcctg
      361 tttggttaaa aggtcggcga taaaaccgaa cacgacacga ccaaaactac tggtgatgcc
      421 ggcaagacca aagatcgtcg cggctagtaa tttgctgtaa cctacatcga ccacgtgggc
      481 gacttggtgg gtcacaatca cggtcgtgcc gcaggccgcg caaatacgcg caaagaacaa
      541 cagccagaac tgccaactcc gcagcgccag ctttgccgtc cagcggtggt catcggactt
      601 cgcttctttc ttcgatcgaa gtttttcgcc gaaaggtcca tgctggtaaa agagccagac
      661 taagggcaac agcaatagca gcacgatccc ggaaaaggtt tgaaaggccc gtggccatcc
      721 ccagctcgat agcaaccatt cactcagcgg cgcgagcagt aagataccta cgccagttcc
      781 agcatagacg aaaccgatcg ccgaggcgcg attcgcaacg aaccactccg aaatcaagaa
      841 tacatggggc accataccgg tgaaggtgat accaacggcg gagatcaaac cgaagaagat
      901 gtagagctgc caaagactgc cgatcgtgct gctcagcaga agtcccaaag ccaggatgaa
      961 gcagccgcta cagacgattt tcttagtccc gaaacggtcc aagagatagc cgacaacggg
     1021 tgagaggatc gcatctacgg tcagaaccat agaaaaaacc ccggcggtgg cgccgcggct
     1081 ccaaccgaag gtgtcaagca aagcgacata gaaaactccg aacgaagaat ggagaccgcg
     1141 ggtaaagcca agaataacga aagcaagcgt agcgacaagc caaggtgtcg gagaaaaacg
     1201 catgacgatg gggcaattat tttctaacag agtgaaccgc cctcaacaag ctaacacggg
     1261 ttgagtttat atcgagccta ttctaataag agctttcatg accaagcaaa atttgatttg
     1321 cgggctgggg cttctactaa tcatcgccgg gtgcgcgact ctagaatcgg gcggggaatt
     1381 cacttccggc cgccgggcgc tgatgagcgg agactcggcg gtcgcgctga cttatttcga
     1441 gccgattgcc aaacgcgatc ccaactacat cgcgccgttt tcttcattca gggagagcat
     1501 ctggacctat ctgggccgcg cgcaatatca gtccggcaag ctcgtcgagg ccaagagttc
     1561 attggaaaga gcgctgtccc aaattcccaa cgattcagtt gctaagcttt acctcgcgtt
     1621 gacgaacctg cgcctgcaaa cgacggagaa agcaaccaat ccctttactc tgcaggacat
     1681 ttcatttgcg ctccgcgagc gggtcgaacc caaacgggtc gccgctcttg tccgggaacg
     1741 cggtgttgct tttgatctta caactgaatc cgagagccaa cttagaaagg ccggcgcgga
     1801 tgactttctg atcgatgaga ttaaaaagat ccgcgccgat gccttgaaac aaagaaagac
     1861 cagcgagagc cagttaggtc aatcgactaa ggaactcgcc gctgctctta ccgccatccg
     1921 cgaccagctg gattacttga acagcacgcg gcaagggata ttctgggatc ctaacaagga
     1981 aattcgttcg caaatccaaa ccgggctttc gcttctctcg gcaccgcagc ccgactggca
     2041 aaaagccctg agcacgggtg aatggatagg acaaagaatc gaggaagaga tcgacctggc
     2101 ccgccgggat gaggccgaag agctacgtcg gcaggaccgc cgctagccgg cggctcgagc
     2161 cgcgaaggcc ttagcaagtt agcccttcac ggcccaatcg ctgttcagcg ttacaggctc
     2221 ttctagtcgc ttcgatttcg tcaaacactt cctggctgcc agggctgatg gcacgcgcct
     2281 tctcgagctc tgccagtgcg gctgcataat ttccttgtac ccggtggctc ttggcccggt
     2341 tgatatagga cgcgatgtta atagtctgga ccggtggttt ggccggcgtt acttggggag
     2401 gctccactgc cggtttgtag gttggcggtt cgggcggtgg tttggcggga gattctctga
     2461 ctaggatgtt caccgctgaa ctttcgattc caccccagcg ggcgatgact gtaattttgc
     2521 cggctcgcaa tgcgagcaac tcgccgcgag aatcaacaga agcaatcgca gtatcgctgc
     2581 tgcgccattc aacgccgctg gaaagtccct tttcgttgcc atcggaatat ctcgccttta
     2641 cgcgaagcga aagccgttcc tgagttcgca cctcacgtct ctccgtgaaa atcgcgagtg
     2701 ccactaatct cgttatcacc ggcgtcttaa ccgccggctt ttctgctggc gctgtgacag
     2761 tcacagcagg cttctccgcg cgcacggcaa gagtccatgc tgggctcacc agatcgccgt
     2821 atcgagccgt aatcttcgtt tccccggcct gccgggctgt gacttgaccc tgatcatcga
     2881 tcgttgccac ccccggttca ctgctgagcc atttgacgcc ccgattgagg ttcttctccg
     2941 ttccatcgct gtattgtcct ttaaggatga catttaacgt ctcgttggcg gcgatttctt
     3001 gacggtcgcc actcaagccg agactaacaa gatttggagc aggaacgggc ggcggcggct
     3061 tcactgccac aacttcgggc cccgaagtgc gcctatcctt ttttgcaaac cacagaaggg
     3121 agcccaggac cgccacgacg attaaagctc caactactgc gattttccat cttgtatccg
     3181 acttgcggtc ggactctatg gtcgtcgatc cgcttcgctg aaccgacggg cttccagcaa
     3241 gtgtcgagtc ttcattgttg cttactgatc tattgacgac tcgagcttcc tctatgggct
     3301 gcggaatcgg tggacttttc cgaggctccc cgtcggtctc agaaagcttt tgaggcggcg
     3361 gctttcccat cagcgattcc aagtcagcaa tcagttgccg ggctccgagg aaatccctcg
     3421 agccgtccca atcgattaga tccgccgcct gaatactgcg aaatccaacc gggggtttga
     3481 cggcttcaat cagtaccggg attagctttc ccacggcgcg agcttcttcg gcttcttctt
     3541 tgacccaatc gctctccacc gagtgagacg accacaggac gaccatgcag cgcatgtcac
     3601 gcagcgcttc ttcgatcatc gaacgccagg tacgaccggc cgggatgcgc cggtcccacc
     3661 agaccgtaca gccggcggat tcgagaaggc cggccacggc acgcgccgct tcccggtctt
     3721 cgttggcata gctgagaaag atatccgcca tagtcccgca aacaatgggc tgaaacagcc
     3781 aagcctagca aacgttctcg atgctggatg tgctgaaacg gacgttagcc gatcttaccg
     3841 taattgtagt cggaaattct accagccgtg gagacctaaa ttggaccggg tctcacgacg
     3901 tatccaacca gaagttgcct cagtgacaaa aaaatcttct gccgttgaca ctcaccggct
     3961 cggcttgata gacgcacaag cagaatgccc gcatgttgag cgaaaaattg tagggaggtc
     4021 ccatggtcat acgagaactg aaggacggct catccatcgt tagcacgcag gaagatcacg
     4081 ccgagctttc ggcacagttt gccgcacact ggggcaacaa ggattttgcc agactgcgtc
     4141 cctataaaac catggtgttt gccacgactt accacgatag cggatatcgg gaatgggaag
     4201 gcaatccgcc gatgaatgtg gcgaaaggac gcccttacgc gcaccgggag gagatcccgt
     4261 cctttgagcc ggtggaactt cgcgcctacg cacgcaacgt cgagtggctg agttcacaag
     4321 attcttacgc cggtcttttg gtatccatgc accgcaccgg gctctggcag aatcgttacc
     4381 aaactttcac gagtcctaag ggcagattgc gcgagcgcag ccccgaggtc aaagccgcaa
     4441 tgcagcaatt ggaagccaaa cagcaagagg agaaaaaatc cctcggcctg ggcaacccga
     4501 cgtttgaaaa cgatctgtgg ttcaattatc gggcgctgca gatctacgat ctgctttcgc
     4561 tttatttctg ctgcgacggt tactctgacg atgatcattt caaggaagag cggctggcgc
     4621 cgattcgctt gtcctatgat agccaggccg aagttgaact gcgcatcgtc cctgccggcg
     4681 tccgctcggt gagaatggaa ccgtacccct tcgacatcgg accactcaca gtgtccgccc
     4741 gggcaagaat cattccgccg gggacatacg catcggaaga agcatgtctg gaagcc
//
LOCUS       DASSWZ010000052         8495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_86523, whole genome shotgun sequence.
ACCESSION   DASSWZ010000052 DASSWZ010000000
VERSION     DASSWZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86523"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VFN58_01225"
     CDS             complement(<1..1110)
                     /locus_tag="VFN58_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8916638.1"
                     /translation="MAKVAQPEPMKQEPVITMWSKEFTYDRWMKTKGIPIHTGYFIPD
                     LRTIELEWWEERGCKAAFIQLLGQEGVSASRVTEIPAGKSLPPVKFALDELVYVLSGR
                     GLTTVWGGESKPKKSFEWQEHALFLVPRNHYHVFSNMQGDKPVRLFHYSYMPMAMSAL
                     PEPDMYFNNPYESKNGATEGDFYSEAKMVQAADADEGLGMRAFWYGNFFPDMRAWNKL
                     DANERRGAGGKSVFIQFPNSELTAHMSVFAPQTYKKAHRHGPGRAIIIPAGEGYSIMW
                     EEGKEKIVCPWHECSMVTPPNKWFHQHFNAGGDMARYLALHPPMQFHGHAEKIEDRAK
                     DQIEYPNEDPYIRQKFEEELGKRGIKSIMPAECYSN"
     gene            complement(1127..1543)
                     /locus_tag="VFN58_01230"
     CDS             complement(1127..1543)
                     /locus_tag="VFN58_01230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET8916639.1"
                     /translation="MADKIRLRVVTPSRLMLDDVVDEVTAPGALGEFGVLPNHIAFLT
                     LLVPGEMSYKDGTTRHYLAVSGGYAEVLDNVMTVLAPAAEFAGEIDTGRARQAKEFAE
                     IRIGELNYEEKEFKTAEAALLWANARLNVAARESRK"
     gene            complement(1586..3001)
                     /gene="atpD"
                     /locus_tag="VFN58_01235"
     CDS             complement(1586..3001)
                     /gene="atpD"
                     /locus_tag="VFN58_01235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET8916640.1"
                     /translation="MTMNTGKITQVLGAVVDVEFSDGSLPPIYNALKVSNPAISDQQG
                     NLILEVAQHLGENTVRCVAMDTTEGLVRGMDAMDTAAGITVPVGAETLGRVLNVIGEP
                     VDEQGPVNAKKSYPIHRPTPEFVDQETKVQAFETGIKVVDLLAPYARGGKIGLFGGAG
                     VGKTVILMELINNVAMKHGGYSVFGGVGERTREGNDLWLEMKESGVINKAALVYGQMN
                     EPPGARARVALTALTVAEYFRDDEGRDVLLFIDNIFRFTQANSEVSTLLGRMPSAVGY
                     QPTLATDLGELEERITTTHKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQI
                     AELGIYPAVDPLDSTSRILDPHVVGQEHYEVARQVQVILQRYKDLQDIIAILGMDELS
                     EDDRLTVARARKIQRFLSQPFHVAEAFTGSPGVYVELKDTIRGFKEVVDGKHDDIPEQ
                     AFYMVGTIEQAVEKAKRMAAA"
     gene            complement(3022..3882)
                     /gene="atpG"
                     /locus_tag="VFN58_01240"
     CDS             complement(3022..3882)
                     /gene="atpG"
                     /locus_tag="VFN58_01240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET8916641.1"
                     /translation="MATLKAIRKRISSIRNTQQITKAMKMVSAAKLRRAQEAAVAARP
                     YAEKMTDLLRNLAARVASNAHPLLTVREEKRVQLVLLTSDRGLCGGYNANLIRAAEAF
                     RREQGPEKEVQLTLIGRKGADYFRRRRAEISDRYVNILATPPEELAAEIAEKLINRFV
                     GGETDAVYILYSSFRSALSQVPTLEKLLPVTVTATAAMEQLTEYLYEPGIQELLASLL
                     PKITEVAIQRALLEATASEHGARMTAMDSATTNASKMMGTLTLQMNRARQASITRELM
                     EIVGTAEALK"
     gene            complement(3929..5437)
                     /gene="atpA"
                     /locus_tag="VFN58_01245"
     CDS             complement(3929..5437)
                     /gene="atpA"
                     /locus_tag="VFN58_01245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET8916642.1"
                     /translation="MEIGTAEISRIIKEQIRDYEKSVEIQEIGTVLSTGDGIARVYGL
                     DKVALGELLEFPHGIFGVALNLEEDNVGTALFGETHMIKEGDTVKRTGRIAEVPVGQG
                     LIGRVVNALGEPIDGRGPIETKDRRRIEIKAPGIVARQPVKEPLQTGLKAIDGMIPIG
                     RGQRELIIGDRQTGKTAVAIDTIINQKGGNVTCIYVAIGQKRSTVAQVVDKLRESGAM
                     EFTIVVAATASESAPLQYIAPYSGCTMGEHIRDNGGHALLIYDDLSKHAVAYRQLSLL
                     LRRPPGREAFPGDVFYLHSRLLERAAKMSDARGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYSGVRPAINVGISVSRVGGNAQIKAIRQVAGTLRLELAQYREM
                     AAFAQFGSDLDQATQRQLNRGSRLVELLKQDQYEPLPVEKQVLIIYAGTNGFIDELPL
                     TALKKYEQELYSFVESKHPDIFADTIKKRELDNDLRAKMNKALDEFKAVFKH"
     gene            complement(5452..5994)
                     /gene="atpH"
                     /locus_tag="VFN58_01250"
     CDS             complement(5452..5994)
                     /gene="atpH"
                     /locus_tag="VFN58_01250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET8916643.1"
                     /translation="MIEGSLSRRYTKALFQLAREAGQEEKISQEIESFLEAYTGAQLQ
                     KVLTNPAFGMDSRKRVVIEVSNRLQLSILAIHFLSLLLERDRLPYLPSIATRYRRLLN
                     ETRGRAEAKIVSPSSLEPVMVDRLQEVLHQISGKEIVLQEETDASLIGGVLVEFEGKV
                     YDGSVRTQLDNMKQRIARGY"
     gene            complement(5991..6539)
                     /locus_tag="VFN58_01255"
     CDS             complement(5991..6539)
                     /locus_tag="VFN58_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET8916644.1"
                     /translation="MSFFELFGVAQAWASAAEAEHHAPSIHQIWFPLGNFLIFAFIIV
                     HYALPLVRNFLQSRRDEVLATVREAAAKKQRAEAIVQDYQGRLARVEQEIQSIAASLR
                     AEGEREKAKLLSDAQTLAVRIKEDARFLADQEVRAARQKIREEMASQAEARARELVQH
                     HLSSADQGRLVGDFIQSIGQVA"
     gene            complement(6536..6964)
                     /locus_tag="VFN58_01260"
     CDS             complement(6536..6964)
                     /locus_tag="VFN58_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET8916645.1"
                     /translation="MISLDYSVVYQIVLFLIVWLILSKVLFRPYLGLLEERERRTLGT
                     QHDSTDLEHEGARLRAQYEEKISQAQAAGYAAKEAILQEARQQRERVLNQAREEAMGM
                     LEGVRREVESQIQKEREFAAAEVRTIAQEMASKILGRNVG"
     gene            complement(7109..7669)
                     /locus_tag="VFN58_01265"
     CDS             complement(7109..7669)
                     /locus_tag="VFN58_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET8916646.1"
                     /translation="MTWFDRSLGGKKEPQKTDRKPDMGQAVSEPAPDENTPSSKLEPA
                     PAPAARGLVGYLYKGSRVTGQLTFQGSARIEGNVDGEIQCHGTLTIGERAEVRAKISG
                     DVVVIRGKVEGNVVAKEKVELVAPARLFGDIDTPRLIITEGVVFDGDCTMGVARQKGG
                     IAGSQNLNADKAAAAKTVKLHVDFEK"
     gene            7836..8435
                     /locus_tag="VFN58_01270"
     CDS             7836..8435
                     /locus_tag="VFN58_01270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET8916647.1"
                     /translation="MIRNLLLIAVLLLGTSIAFAGEAQDKKGPVYATLKTSMGDIVIQ
                     LFEDKAPKTVANFIELASGTKEWTDPKTGAKVKRPLYNGTIFHRVIPGFMIQGGDPLG
                     NGTGGPGYRFEDEFNPDLKHSKPGVFSMANAGPNTNGSQFFIIQKATPWLDGKHSVFG
                     EVVKGQNVVDAIVNVPRDFRDRPIKEVVLQEVIISRGSY"
BASE COUNT         1827 a         2479 c         2179 g         2010 t
ORIGIN      
        1 attggaataa cattcagcgg gcatgatgct cttgatgccc cgcttaccca gttcctcttc
       61 gaacttctga cggatatacg gatcctcatt gggatattcg atctggtctt tggcgcggtc
      121 ttcgattttc tccgcatgac catgaaactg catcggcgga tggagcgcca ggtagcgcgc
      181 catgtcgccg ccggcattga aatgctggtg gaaccactta tttggcggcg tgaccatgct
      241 gcattcgtgc cacgggcaga cgatcttctc tttgccttcc tcccacatga tcgaataacc
      301 ttccccggcc ggaatgatga tggcacgacc cggaccatgc cggtgcgctt tcttgtaagt
      361 ctgcggcgca aaaacagaca tatgggcagt cagttctgag ttgggaaact ggataaaaac
      421 gctcttgccg ccggcaccgc gccgttcgtt ggcatccagc ttgttccacg cccgcatatc
      481 cggaaagaaa ttaccgtacc agaacgcccg catgcccaaa ccttcatcgg cgtcggccgc
      541 ttggaccatt tttgcctcgg aataaaaatc cccttcggtt gcgccatttt tcgattcgta
      601 ggggttattg aaatacatgt caggttccgg cagcgcggac atcgccatcg gcatataact
      661 gtagtggaac aaccgcaccg gtttgtcgcc ctgcatattg ctgaaaacgt ggtaatgatt
      721 gcgtggaacc aagaatagag catgttcctg ccactcgaag cttttcttcg gcttgctttc
      781 tccaccccaa accgtcgtga ggccgcgacc cgacaagaca tagacgagct catccaaagc
      841 aaatttcacc ggcggcagcg atttgccggc ggggatttcc gttacccgcg acgcactgac
      901 gccctcttgc ccgagcagct ggatgaaagc cgctttgcat ccccgttctt cccaccactc
      961 cagctcgatg gtacgcaaat ccgggataaa ataaccggta tgaatcggaa tccccttcgt
     1021 tttcatccac cgatcgtagg tgaattcttt gctccacatc gtaatgacgg gttcttgttt
     1081 catgggttcc ggttgagcca ctttcgccat aagacctcct ttttagctat tttctcgatt
     1141 ctctagcagc gacgttgagt cgggcattcg cccaaagaag tgctgcctcg gcggttttga
     1201 actctttctc ttcgtagttt agctctccta tcctaatttc agcaaactcc ttagcttgac
     1261 gcgcgcgacc tgtatcgatc tcgcctgcaa attcggctgc aggcgccagt acggtcatga
     1321 cattatccaa cacctcggca tagcccccgc tgaccgccaa gtaatgtctg gtcgttccgt
     1381 ctttatagct catctcgccg ggcaccaaaa gagtaagaaa agcgatatgg ttcggcaaaa
     1441 caccgaactc gcccaacgcg cccggggccg tgacctcgtc gaccacatca tcaagcataa
     1501 gccggctcgg cgtcaccact ctgagtctga ttttgtcagc catcttttct cagcgacaaa
     1561 cgtcgatatc tttgccgcac ccgcgctagg ccgctgccat tctcttggct ttttctactg
     1621 cctgttcgat cgtcccgacc atgtagaacg cctgctcggg gatatcgtcg tgtttcccat
     1681 caaccacttc tttgaagccg cgaatggtgt cttttaattc aacataaacg ccagggctac
     1741 ccgtaaaagc ttcggcgaca tggaacggtt gcgacaagaa gcgctggatc tttcgcgcac
     1801 gggccacggt taacctgtca tcttccgaga gctcgtccat accgagaata gcgatgatat
     1861 cttgcaagtc cttgtagcgt tggaggatca cttgcacctg ccgtgcgact tcgtagtgct
     1921 cctggccaac aacatggggg tccaagatac gggaggtcga atccagcggg tccacggccg
     1981 ggtaaatacc taactccgcg atctgacgcg aaagaacggt tgtcgcgtca agatgagcaa
     2041 aggtcgtcgc cggcgcggga tcggtcaggt cgtcggctgg cacgtagatc gcttgaaccg
     2101 aagtgatgga gcctttatgc gtggttgtga ttcgttcttc caattcaccc aagtcggtgg
     2161 ccaaggtcgg ctgataaccg acggcagaag gcatgcgtcc gagcaaggtc gaaacctctg
     2221 agttagcctg ggtgaaacgg aaaatgttat cgataaagag cagcacgtcc cggccttcgt
     2281 cgtcgcggaa gtactccgcc acggtgagcg ctgttagggc gacacgggcg cgggcgccgg
     2341 gaggctcatt catctgtccg taaaccagcg cggccttgtt gataacgccg gattccttca
     2401 tctcgagcca gaggtcgttg ccctcgcggg tccgttcgcc gaccccgccg aagacggaat
     2461 agccgccgtg cttcatggcg acgttattga tcaattccat gagaataaca gtcttgccga
     2521 caccggcgcc cccgaacagg ccgatctttc cgccgcgggc ataaggagcg agcaggtcga
     2581 cgaccttgat accggtttcg aaggcctgga ctttagtttc ttgatcgacg aattccggag
     2641 tggggcggtg aatcggatag gattttttcg cgttgactgg cccctgttca tccacaggct
     2701 caccgatgac gttgagcacg cgccccagtg tttccgcacc gaccggtacg gtgatgccgg
     2761 cggcggtatc catcgcatcc ataccgcgaa ccagtccttc ggtcgtatcc attgcaacac
     2821 accggacggt attttcaccc aagtgctgcg ccacttccaa gataagattc ccttgctgat
     2881 cgctgatcgc cggattgcta accttgagcg cattgtaaat gggcggcagc gagccgtcag
     2941 aaaattccac atcgacgacc gctcccaata cctgggtgat cttccccgta ttcatagtca
     3001 ccattccctc cgttcaattc ctcacttcaa agcttccgct gtaccgacga tctccatcag
     3061 ctcgcgcgtg atcgaagcct ggcgcgcgcg gttcatctgc aaggtcagtg tccccatcat
     3121 cttcgatgcg ttcgtcgtcg cggaatccat ggccgtcatg cgcgcgccat gctcgctggc
     3181 ggtcgcttcc agcaaagccc gctgaatagc tacctcggta atcttgggca gcagactggc
     3241 taaaagctcc tgtatccccg gctcgtaaag atactcggta agctgttcca tcgcggccgt
     3301 tgccgttacc gtcaccggca aaagtttctc cagagtcggc acttgggaaa gagctgaccg
     3361 gaaactgcta tagaggatat agaccgcatc ggtttcgccg ccgacgaaac ggttgatcag
     3421 cttttcggcg atttccgccg ccagttcttc ggggggagtt gcaagaatat tcacgtagcg
     3481 atcggagatc tcggcgcggc ggcggcggaa ataatcagct cccttacgac caatcaatgt
     3541 aagctggact tccttttcag gaccttgctc acgtctaaag gcctcagcgg cacggataag
     3601 attggcgttg tagccgccgc acagcccacg atccgatgtt aacaacacaa gctggactcg
     3661 cttctcttca cgcacggtga gcagcgggtg ggcgttgctt gcgactcgag ccgcaaggtt
     3721 tcttaataaa tcggtcattt tctcggcata gggacgcgcc gccaccgccg cctcttgcgc
     3781 gcgtcgcagt ttggcagccg acaccatctt catcgctttg gtgatctgct gcgtattacg
     3841 gatcgagctg atccgcttgc gtatcgcttt aagggtcgcc attgtccctt acaagttcag
     3901 ggttctgagt taatcagaaa cctgaaatct aatgtttaaa aaccgcttta aactcgtcca
     3961 gcgccttgtt catcttggcg cggagatcat tatccagttc gcgcttcttg atagtatcag
     4021 caaaaatatc cgggtgcttc gactcaacaa aagaatagag ctcctgttcg tacttcttga
     4081 gggccgtaag cggaagctca tcgatgaatc cgttggtgcc ggcgtagatg atcaagacct
     4141 gtttttctac cggcagcggc tcgtactgat cttgcttgag tagttccacg agacggctcc
     4201 cacgattgag ctggcgctgg gtcgcctggt ccaagtccga tccgaattgc gcgaaggccg
     4261 ccatttcacg gtactgcgcg agttccaacc ggagggtgcc cgccacctgt cttatcgcct
     4321 tgatctgggc gttgccaccg actcgagaga ccgagatacc gacgttgata gccggcctga
     4381 caccggaata gaaaagatcg ctttccagaa agatctggcc gtcggtgatg gaaataacat
     4441 tagtcggtat ataggcagaa acatcacccg cctgggtctc aatgatcggg agcgccgtca
     4501 gcgatccacc accgcgggca tcgctcatct tcgcggcacg ctccagcaac cgagagtgga
     4561 gataaaacac gtctccggga aatgcctcac gtccgggcgg ccgacgcagc agcaaagaga
     4621 gctgacgata ggcaacggcg tgtttcgaaa gatcgtcata gatcagcaga gcgtggccgc
     4681 cattgtcacg aatgtgctcg cccatggtgc aaccgctata gggagcgata tattggagcg
     4741 gagccgactc ggaagccgtc gcggcgacta caatggtaaa ctccatggcg ccggattctc
     4801 ggagtttatc gacgacttgt gcgacagtcg atcgcttttg gccgatggcg acgtagatac
     4861 acgttacatt gccgcctttt tggttgatga ttgtgtcgat ggccaccgcg gtttttcctg
     4921 tctgccgatc gccgatgatc aattcccgct gcccgcgacc gatgggaatc atgccgtcaa
     4981 tagccttcag tcccgtttga agcggctcct taaccggctg acgcgcgacg atacccggcg
     5041 ctttgatctc gattctccgc cgatccttgg tttcgatggg tccccggccg tcgattggct
     5101 cgcccaaggc attgaccacg cgtccaatca atccctggcc cacaggcacc tcggcgattc
     5161 taccagtgcg cttgaccgtg tcaccctctt tgatcatatg ggtttcgccg aaaagggcgg
     5221 ttccgacatt gtcttcttcg aggttcagcg cgacgccaaa aataccgtgg ggaaattcca
     5281 acagctcacc gagcgcgact ttgtccagcc catagacgcg ggcaatgcca tccccagtcg
     5341 aaagaaccgt tccgatctcc tggatctcaa ccgacttctc gtagtcccgg atctgttcct
     5401 ttatgatgcg gctaatctcc gctgttccga tctccataaa tagatcgctc cttagtatcc
     5461 gcgcgcgatg cgctgtttca tattatcgag ctgtgtccgc acactgccgt cataaacctt
     5521 gccttcgaac tctactaaaa caccaccgat gaggctggcg tccgtctctt cctgcagcac
     5581 tatttctttg ccggagattt ggtgcagtac ttcctgcagc cggtccacca tcaccggctc
     5641 cagcgagcta gggctgacaa ttttggcttc cgcgcgaccc ctggtctcat tcagcaagcg
     5701 ccggtagcga gtagcaatcg agggaagata agggagtcga tcacgctcca gcaaaagcga
     5761 caggaaatga atcgccaaaa tcgaaagttg cagccggttc gatacttcga tgacgactct
     5821 cttacggctg tccatcccaa aggcgggatt cgtcaggacc ttttgcaact gcgcgcctgt
     5881 ataagcctcg agaaagcttt caatctcttg gctgattttc tcttcttgcc ccgcctcgcg
     5941 agccaattga aagagcgcct tggtatatct ccgacttaaa ctgccttcga tcatgcaacc
     6001 tgtccgatgc tttgaatgaa atctccgacc aagcgacctt gatcagccga agaaagatgg
     6061 tgttggacga gctcgcgagc cctggcctcg gcctggctgg ccatctcctc acggattttt
     6121 tgccgcgccg ctctgacttc ttggtcagcc aggaaccgcg cgtcctcctt aattctgacg
     6181 gcgagcgttt gcgcatcgct caagagcttt gctttctccc tttcgccttc agcccgcagc
     6241 gacgctgcga tcgactgaat ttcctgctcc acccgcgcca accggccctg gtagtcttgg
     6301 actatcgcct cagcccgctg ttttttcgca gcagcctccc ggaccgtagc gagcacttca
     6361 tcgcgccggg attggaggaa gttacggact agcggcagcg cgtaatgaac aatgatgaaa
     6421 gcaaaaatga gaaaattccc gagcgggaac caaatttgat gaatggatgg tgcatggtgc
     6481 tcggcttccg cggccgaagc ccatgcctgt gcaacgccaa agagctcgaa aaaactcacc
     6541 ccacgttcct cccgaggatc ttgctcgcca tttcctgtgc tatcgttctg acttcagctg
     6601 cggcgaattc ccgttctttc tgtatctggc tctccacttc ccgccgtacc ccttccagca
     6661 tccccatcgc ttcctcgcgg gcctggttca aaactctttc acgttgctgg cgcgcctcct
     6721 gtagaatcgc ctccttggca gcgtagccgg cggcctgcgc ttgggagatt ttctcttcgt
     6781 attgtgcgcg caatcgcgcc ccttcatgtt ccaagtctgt tgaatcgtgc tgggttccga
     6841 gggttctacg ttctcgttct tccagcagac ctagataggg acgaaataaa accttactca
     6901 ggatgagcca aacgataaga aaaagaacaa tttgataaac gacggagtag tctaaggaga
     6961 tcattgggtc actttggctg tcaattcgtt tttgtttcag tccgatcggg caatgagcaa
     7021 ttaaacgccc aattggaaaa aaccgccttc acaatcggtc tatctatgaa cttaggatgc
     7081 agggatggga tgaaccgcga aacgaagtct atttctcgaa atccacgtgc agcttcaccg
     7141 ttttagctgc cgcagcttta tcagcattta agttctgtga acctgcgatt ccaccttttt
     7201 gcctcgccac tcccatggtg cagtcaccgt caaacacgac cccctccgtg atgatcaatc
     7261 gaggagtatc gatatcacca aacaatcgag ccggcgccac caactccacc ttttctttgg
     7321 ctactacgtt gccctctact ttgccgcgga tgactacgac atcgccggag attttggccc
     7381 gaacttcagc ccgttcgccg atggtcaagg ttccgtgaca ctggatttcc ccatcaacgt
     7441 tcccctcgat cctggcagac ccctgaaatg tcagctgacc cgttactcga ctccctttgt
     7501 aaaggtaacc caccagcccg cgcgccgcgg gggccggcgc cggttccaat ttcgacgatg
     7561 gtgtgttttc atccggagca ggctccgaaa cggcctgtcc catgtccggc ttccgatcgg
     7621 ttttctgagg ctcctttttg ccgccaaggc ttcgatcaaa ccatgtcata aaaagagctc
     7681 ccttgctcag gacaaaattg gtctatagca tccaggcaac ctattgtcaa aagatagagt
     7741 ctcacgatag tgtatttaat tgctacgcaa attgggacat atactcggat tcgtcaacga
     7801 agtcgtcgcg gaactgggac agaaatgagg agatgatgat tagaaacttg ctgttgatcg
     7861 cagtattgtt gctcggcacg tcgatcgcgt tcgccggcga ggctcaagac aaaaaaggac
     7921 cggtttacgc gacgttaaaa accagtatgg gcgacattgt gattcagcta tttgaggaca
     7981 aggcaccaaa gactgtcgcc aacttcatcg agttagcgtc gggcaccaag gaatggaccg
     8041 atccgaagac tggagcgaaa gtaaagcggc cgctctataa cggcacgatc tttcatcgcg
     8101 tcatccccgg ctttatgatc caggggggcg atcctttggg caacggcacc ggtggtcctg
     8161 gatatcgctt cgaggacgaa ttcaatcccg atctcaagca ctctaaaccc ggcgtctttt
     8221 ccatggctaa cgccggaccc aacaccaacg gtagccagtt ctttatcatc caaaaagcga
     8281 ccccatggct cgacggcaaa cacagcgtgt tcggcgaggt cgtaaaaggc caaaatgtcg
     8341 tcgacgccat cgtcaacgtg ccgcgggatt ttcgcgatcg gccgattaag gaagtagtgc
     8401 tccaggaagt gatcatctcg cggggaagtt actaaccacc gcacgcggcg acgactccgg
     8461 gtgccagaaa accaatgtgg ttacttcgtt tcggg
//
LOCUS       DASSWZ010000053         6395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1232630, whole genome shotgun sequence.
ACCESSION   DASSWZ010000053 DASSWZ010000000
VERSION     DASSWZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232630"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..213)
                     /locus_tag="VFN58_01275"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="VFN58_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941054.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(709..1779)
                     /locus_tag="VFN58_01280"
     CDS             complement(709..1779)
                     /locus_tag="VFN58_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916648.1"
                     /translation="MITIPYAASLTILVFCLLLVSGTAVCAVSVEERLDQLNRQAGKQ
                     RTETLEREARKEGEVVWYAAMASDRAAELIRAFESNYTFLKVRFQPGGASRQLDQLLV
                     EHRSKKHRADIINTRRSFVGVMAKNGAIARYRTPLRASLRDGFTDKEGYVNGIYAQPR
                     VFLFNSRIVPRDKAPKSFEDLLDPQWKDKLGMDTTDYDWIAALIDYYGQSKAIEFAGQ
                     LSKQQLNMRRGPTLLAQLAVAGEFPIVIDAFPEEVLQLKNAKAPVDFTFSEPFVAVKT
                     PTTVSISAGAPHPNAAALFVDFLLSKPGQEIMASQGRWVSRNDVGYLVDLKGKRMQIP
                     SPAWDDKQVELIKLYNSLFSLR"
     gene            complement(1802..2782)
                     /locus_tag="VFN58_01285"
     CDS             complement(1802..2782)
                     /locus_tag="VFN58_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916649.1"
                     /translation="MRRIVFAVFVSLVTSAALGNAQLAPIRISYATTSGIRSPLWIAE
                     DARLFEKYGLDAKLINIPSGNTAISALVTGEVDVVSGSGSASIVAAGRGLPVVIVGSF
                     GSTTYKLVANPGITDLRGKIVGTSRIGSTTDFALRRTLSMLGLTPDKDVKILATGIGE
                     ADKRIMLMLQGRMDGTIGSPESIFAAETQAKVKIEILADLEEMKIYNTVGDISTRTDV
                     LKNRRDLLRSFFMACSEAIALGKKNKATAQRVLAKHMKITDRKRLDIVYEASLGRMPS
                     KPYPREEAVQLELESVAFTDPLFKTKRVSEFMDSSIIAELERQGFFIQLK"
     gene            complement(2787..3779)
                     /locus_tag="VFN58_01290"
     CDS             complement(2787..3779)
                     /locus_tag="VFN58_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8916650.1"
                     /translation="MKREREDPKQPELSAENYYDYEARFVKQKREQERINVLPRVIKP
                     AMFAKGEFALGDMRVFSRYTVAPLSSLTCGFVELKPGAHSDPKRMVPSAIAYVLEGSG
                     ESIQENARFSFAAGDVVIIPPYTTHQFAANPDEGFRAWLPQVRIWHGQGLLWREQSEA
                     DRGHEAIFQARRRVAIPPATRTRYDWFLGRLAEENRIEREAPRVIRGTDCSWESTRQG
                     KLKFCISRWTEVAAPALDLMVQEIRPGERSGEHRHIFEELLLVVKGRGYDVHEGTRHP
                     WEAGDLICIPPMISHQHVNDGTDAAQLVSVWSRQPGHEYLGGIEQLSDASDWIP"
     gene            complement(3798..4943)
                     /locus_tag="VFN58_01295"
     CDS             complement(3798..4943)
                     /locus_tag="VFN58_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8916651.1"
                     /translation="MEERWPWEAEESLEPSDPLAHQRLEESFKEQLPRVIKLLPVQGT
                     VMGSSSPHQTGDIVCYDILKRAPLYDLTSHFSDIHPHAPVRGAHRHISAPTLFCLSGR
                     GWEINDGKTYWFSTYDMLVVPPFTIHQHGGDEEAGCEIFVPQSRMFSILGLTKREQIK
                     FGEKPTFPQGTEALHDELGKLVGYRIKKGVLGIKEDIDVMLGADQKTEAVFRARKEVI
                     PWEGAVENTYDRYLKMFHDEGSFCNAVTHVISFEEQSWEWCRQGRLKWFTHPEMESAA
                     RRVWLYIQEISPGSRSGKHRHVAEEQILVISGRGYDIQDGKRWDWERGDLINIPSMTE
                     HQHFNADTEEPVLLLSSMPSVCTDLGLGGIEQLEDAPEYMDSKQQSD"
     gene            complement(5082..6083)
                     /locus_tag="VFN58_01300"
     CDS             complement(5082..6083)
                     /locus_tag="VFN58_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916652.1"
                     /translation="MSKHGRCIRLLIFVLLSHGVVFAVETQLPLANVTIAYTSISPQY
                     APVWIAKEAGFFKRNGINAQLVYMRGGIVATQALVSNDVNFINAGGGGVVDAVLGGAD
                     IFIVASPINQEPQVLVAKKEIKEIAQLKGKKLAVNSLAGPSILSLKMILAASGLDPDR
                     DVSYLATGPSASRFGALQLGQVDATTLTPPFTSAARRAGYTFFESIPGMKEAELPNAA
                     LITSRKFFEAEPLAAEMVIKSVIEGIHFYKTEKIKTPAILKKYMKLENAEELQDTYNF
                     YVKLLAEKPYPTAKGIQTILEWSKRADARKANPAQFIQTRVVEKLDKEGFIDGLYKK"
     gene            complement(6099..>6395)
                     /locus_tag="VFN58_01305"
     CDS             complement(6099..>6395)
                     /locus_tag="VFN58_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8916653.1"
                     /translation="ASAAVQLNLDKDGACSEIGIGVTGAGIVPQKAVSVETLLRGRQV
                     TSALIDQTGQLIQEGAEPIEDLRGSAEYKKKALSTILRRAIAESLRRAKDKQSL"
BASE COUNT         1384 a         1846 c         1603 g         1562 t
ORIGIN      
        1 gtcgccgcgt gatttcctga tcttcctggc tcggtgcagg gtcgccgctt gggtggttgt
       61 gaacaaatat gacggcagca gcgcaaaggt gaagcgccga gcgccatact tcgcgaggat
      121 gcacgagcga tgcagtcagg cttcccgtcg atttaccttg aatccgttca ggcggttctt
      181 gttgttcatg gcaagtagta gaaaatgctc cttgtccgcg tctagtgttt gatagatagc
      241 acgcgctagc cgttcgacat ccgccgacgc cctacacggc gaaccagtgg gaaactcagg
      301 ctctgctatg aattgcactc gaaacgtttt taatcgataa gtcattgccg tctcctcgtt
      361 cgaaagagac ggacaattca cggaccgccg ttgaaaaatg tttgtgctgc acagggttaa
      421 aaaaggcggc tggttctgac aaccagttcg gggcttacgc gcctgcccgg gtgtgtccgc
      481 cttttgtccg tgtgccacaa aagacaagct ccaagaactt acaaccacgc agacctttaa
      541 agcttgagat tgttgggatt tttgggagcg tttagtctgt agtgacgggt aggaagggtc
      601 ttcaaaacca gtctgggggt cgtaaggtcc ccggagggtt cgactccctc ccttctccgc
      661 cattaactca ggcaggaccg ttgccgatag ctttttgatt cttgaaggtt agcgaagcga
      721 aaagagacta ttgtacagct tgatcagctc cacttgttta tcgtcccaag cgggagaagg
      781 aatctgcatc ctcttacctt ttaggtcgac taaatagccg acgtcatttc gactcaccca
      841 gcgaccctgg ctcgccataa tttcctgacc cggtttggaa agcagaaaat cgacaaacag
      901 agcggcggcg ttagggtgtg gtgcgccggc ggaaatggaa accgtcgtcg gtgtcttgac
      961 cgcgacgaaa ggttccgaga atgtgaaatc cacgggtgct ttggcattct ttagctggag
     1021 cacctcttcg ggaaaggcat cgatgacgat gggaaactcc ccggccaccg ccagttgcgc
     1081 caaaagcgtt gggccacggc gcatattgag ttgctgtttg gagagctggc cggcaaactc
     1141 tattgctttg ctttggccat agtagtcgat gagcgccgca atccaatcgt agtccgtcgt
     1201 atccataccc aacttgtctt tccattgggg gtcgagcaga tcctcgaagg acttaggtgc
     1261 cttatcccgc gggacgatgc ggctattgaa taaaaagact cttggctgcg catagattcc
     1321 gttcacataa ccttctttat cggtgaagcc atcccgcaag gacgctctca agggcgtgcg
     1381 gtagcgggcg atggcgccgt tctttgccat cacaccgacg aaggaacgcc gcgtattgat
     1441 aatgtcggct cgatgtttct tgctgcgatg ttccacgagc aactgatcga gttgccggct
     1501 cgctccgcct ggttgaaatc tgacttttaa gaaggtgtag ttgctttcaa atgctctaat
     1561 gagttccgca gcgcggtcgc tcgccatcgc tgcgtaccaa acgacttcgc cctctttgcg
     1621 cgcttctctt tcaagcgttt cggtacgctg tttgcccgct tgccgattga gttgatccaa
     1681 gcgctcttcc acggacaccg cgcacaccgc cgtcccggaa acaagcagga gacaaaaaac
     1741 gagaatcgtt aaacttgccg catagggtat cgttatcacg cctcgctctc ccctgtctca
     1801 gctatttcaa ttggatgaaa aagccttggc gctccagttc cgcaatgatg ctgctatcca
     1861 tgaactcgga cacgcgctta gttttgaata gaggatcggt aaaagccacg ctttcaagct
     1921 ccaactgaac ggcttcctca cgcggatagg gtttgcttgg catccggccc aacgaggctt
     1981 cgtatacgat gtcgagccgc ttacgatcgg ttatcttcat gtgcttggcc aatacccgct
     2041 gcgcggtcgc tttgttcttc tttcccaaag cgatcgcctc gctgcacgcc atgaaaaatg
     2101 accgcaggag atcacggcgg tttttcagca catcggttct tgtcgagata tcgcctacgg
     2161 tgttgtaaat tttcatctct tcgaggtcgg ccaaaatctc gattttcact ttcgcctgag
     2221 tctcagcagc gaaaatgctc tccgggctgc caatggtgcc atccatccgt ccttgcagca
     2281 tcaacatgat tctcttatcc gcttcgccaa tgcctgtagc caaaatcttc acgtctttat
     2341 cgggggtaag cccaagcatt gataacgtcc gccgcagcgc gaaatccgtt gtgctgccga
     2401 tccggctcgt gccaacgatt ttgccacgca gatccgtgat gccgggattc gcgaccagct
     2461 tgtacgtcgt gctgccaaaa ctgccgacaa tgaccaccgg cagtccgcga ccggccgcaa
     2521 cgatagaagc cgagccggac ccgctcacga catcgacctc tccggtaacc aacgcgctga
     2581 tggccgtatt gccggaagga atgttgatca gcttggcatc caagccgtac ttctcgaaga
     2641 gtcgcgcgtc ttcagcgatc cacaacggcg agcgaatccc tgaagttgta gcgtaggaga
     2701 tcctgatcgg agccagttga gcgttgccta gggctgcgga ggtgacgaga gaaacaaaaa
     2761 ctgcaaagac aattcggcgc atgttttcac ggtatccagt ctgaggcgtc actgagctgc
     2821 tctataccac cgaggtactc atggcccggt tgacgagacc aaacggagac aagttgagcg
     2881 gcatcggtcc catcgttgac atgctggtgg ctgatcatcg gcggaataca aatcagatca
     2941 cctgcctccc agggatgtcg cgtgccttcg tgaacgtcat aaccccggcc cttcaccacc
     3001 aacaagagct cctcgaaaat gtgacggtgt tcaccgctac gctctcccgg acggatctcc
     3061 tgtaccatta gatccaaggc cggagctgcc acttctgtcc atcgactgat acaaaacttc
     3121 agttttcctt gccgcgtcga ttcccaagag cagtccgtcc cgcggatcac acgaggagct
     3181 tctctctcta tccgattttc ttccgcaagt ctgcctaaga accaatcgta ccgagtccgg
     3241 gtggccggag gaatcgcaac tcgtcgccgc gcctgaaaga tggcttcatg ccctcggtcc
     3301 gcttctgact gttcccgcca taaaagccct tggccgtgcc aaattctcac ttgcggcagc
     3361 caggcgcgaa acccttcgtc ggggttcgcg gcaaattgat gcgtcgtgta cggcggaatg
     3421 ataaccacgt cgccggccgc aaacgagaat cgagcgttct cctgaatgga ctctccgctt
     3481 ccctcgagaa cataggcaat agctgaaggg accatgcgct tgggatccga gtgtgcgccg
     3541 ggcttcagct cgacaaatcc gcaggtcagg gaactaagcg gcgctaccgt atagcgacta
     3601 aacacgcgca tatcccccag cgcgaactca cccttggcaa acatggcggg cttaatgacg
     3661 cgcggcaaga cgttgatacg ctcttgttct cgtttctgtt taacgaagcg cgcctcatag
     3721 tcgtagtaat tctccgccga cagttccggt tgtttcggat cttcccgctc tcgtttcatc
     3781 tcggccacat tttttcgcta gtcgctctgc tgtttcgaat ccatatattc cggggcatct
     3841 tcgagctgct cgattccgcc gagacctaag tccgtacaaa cgcttggcat agacgatagc
     3901 agcaagacag gctcttcagt atccgcattg aagtgttgat gctccgtcat agaggggatg
     3961 ttaatcagat ctccccgttc ccagtcccac cgtttgccgt cctggatgtc atagcctcgc
     4021 ccgctaataa caagaatctg ttcctccgct acatgccgat gcttgccgga gcgacttcct
     4081 ggactaattt cctgtatata tagccagacc cttctagccg cgctctccat ttccggatgc
     4141 gtaaaccact tcaaacgacc ctgccggcac cattcccagg attgttcttc gaaggaaatc
     4201 acatgagtca ccgcattgca gaaggagcct tcgtcgtgaa acattttcaa gtatcgatca
     4261 taggtatttt cgactgcgcc ttcccatgga atcacttcct ttcgcgccct gaacaccgcc
     4321 tccgtctttt gatcggcgcc cagcataaca tcgatatctt ccttaattcc gaggactcct
     4381 tttttgatcc gatacccgac gagcttccct agctcatcat gaagggcctc cgtcccctgg
     4441 ggaaaagtcg gtttctcgcc gaacttaatt tgctcccgtt tggttaaacc gagaatactg
     4501 aacatccggc tttgcggcac gaagatctca cagccggcct cctcatcccc accatgctga
     4561 tgaatagtga agggcggcac caccagcatg tcataggtgc tgaaccaata ggtcttgccg
     4621 tcgttgatct cccaacctcg gccgctcaag caaaacaagg tcggcgcgct gatatgccgg
     4681 tgcgctcctc ggaccggcgc gtgggggtga atatcggaaa aatgggacgt gagatcatag
     4741 agcggcgcgc gtttcaggat gtcatagcaa acgatatccc cagtctgatg ggggctgctg
     4801 cttcccataa ctgtaccttg caccggcagc agttttatta cacgaggcaa ttgctccttg
     4861 aaagattcct caaggcgttg atgcgccaac ggatcgctag gttctaaact ctcctccgcc
     4921 tcccagggcc aacgctcttc cattatggag cctcgtttct atctgtcgta tcgcctaggg
     4981 catccgccga gcgacagttt tcatcctgca ttcccaaagt gacagtccaa acattctgac
     5041 gacaatgcaa atgcactttt gaggtccaaa catctcgctc gctacttctt atacaacccg
     5101 tcgataaacc cttccttatc cagtttttca accaccctcg tctgaatgaa ctgcgccgga
     5161 ttcgccttgc gcgcatccgc ccttttcgac cactcgagaa tggtctggat acctttagcg
     5221 gttggatacg gtttctccgc cagcaacttc acgtagaaat tataggtgtc ttggagctcc
     5281 tcggcatttt caagtttcat atacttcttc aggatcgcgg gcgttttgat tttttcggtt
     5341 ttgtaaaaat gaattccctc gatcaccgac ttgatcacca tctccgccgc caatggttcg
     5401 gcctcaaaga acttccggct ggttattagc gcggcattag gcaattccgc ttccttcatt
     5461 ccgggaatac tttcaaaaaa ggtgtagccc gctctgcgcg ccgccgaggt aaacggcggc
     5521 gtcaacgtcg ttgcatctac ctgtcccaat tgcagcgccc cgaacctgct ggccgatggt
     5581 ccggtggcca aatagctcac gtcccgatca ggatccaacc cggaagccgc cagaatcatt
     5641 ttcaacgaca atatcgaggg accagccaga ctgttcacag cgagcttttt gccttttagc
     5701 tgggcgatct ctttgatctc ttttttcgca accaagactt ggggttcttg gttgattgga
     5761 ctggcgacaa tgaagatatc cgcgccgcca agcacggcat ccacgactcc accgcccccg
     5821 gcattaatga agtttacgtc gttgcttacc agtgcctggg tcgctacgat tccgccgcgc
     5881 atgtaaacca actgtgcgtt aatcccattt ctcttgaaaa accctgcttc tttggcgatc
     5941 cagaccgggg cgtactgagg cgaaatcgaa gtataggcaa tcgtaacatt ggcgagcggc
     6001 agttgggttt ccacagcgaa gactacgccg tgcgacaaca aaacaaatat tagcagccga
     6061 atgcaacgac cgtgttttga caagatgctg ctccctgctt acaaagactg cttgtctttt
     6121 gcccgccgca gactttcggc gatcgctcgg cgtaaaattg tgctgagcgc tttctttttg
     6181 tactccgccg agccccgcag gtcttcaatt ggctcagcac cttcctggat gagttggcca
     6241 gtctggtcga tcagcgcgga agtaacttgc ctaccgcgga gcaaagtttc caccgacacg
     6301 gctttctgtg gcacaatgcc ggcgccggtc acgccaatac cgatttcact acaggcgccg
     6361 tctttgtcga gattgagctg caccgcggcg ctggc
//
LOCUS       DASSWZ010000054         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_992082, whole genome shotgun sequence.
ACCESSION   DASSWZ010000054 DASSWZ010000000
VERSION     DASSWZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992082"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..2592
                     /locus_tag="VFN58_01310"
     CDS             103..2592
                     /locus_tag="VFN58_01310"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET8916654.1"
                     /translation="MGTVLRQRRWMLQFPPETLVAVLLGAFLFIPSILSNAAEQKNAE
                     ATKTPPLTTIPVEEIATQATQVGDLIRGFAANVVPTGEIETIRKFLPQVSVDIALELK
                     NTTNILQEQASLETLQAQEEIWQQRQSQGTSWLKVLTDRATKLQVALTQLKKLHETWA
                     KTRDAEESAKAPPPILQQIDTTLAVIEAAQQPHQAQRDEILNLQSKVAELVASCNTAL
                     AQIAGLQHMAVGGIFTPENPPIWNGDLWARAKITLPERVPKIAASYWGDILLYVRDPS
                     RHLPWHVAIFIALSLVLLAARRQVGQGDADGDRSSHATAVFDHPFAAALLVTLMIATS
                     PASPVPVTVKRLFQIAALVPIIVLTRRMVTPIVVPVIYALGILFATDTVRRAVVGEPP
                     MGQGILALEAVAGIVVLGWLWVYGARRAARGLGQTSTLRRLVAAFILFSLAIGFIAAV
                     FGYMRLARLTIPGVFAGGALALELYASAQVAMGLIVYALRLWPLRLLHMVQHHRDLME
                     RRIYRLLLWLVAGGWLLRYLDYLGLLDPAWSLATAFLNTRLERGSISTSVGDIVAFFL
                     TVLAAYLLSAFIRFVLAEDVYSRTGIATGQSYAVSSLLNYAILAIGFVVALGVLGMDL
                     TKVTVLAGAFGVGIGFGLQSVVNNFVSGLILLFERPIHVGDTVQVGSLQGRVRRIGIR
                     ASIVRTMQGAEIIVPNAQLITEQVTNWTLSDQLRRLDLPVGVNYGAAPKKVIELLEAV
                     GRAHPKVLQEPAPRGLFMSYGDSSINFELRAWTEQTNSVQVHSDLTVAIYDAIYAAGM
                     SFPFPQREVRVLSDHGGESTMTPVRAADKKT"
     gene            complement(2603..>2924)
                     /locus_tag="VFN58_01315"
     CDS             complement(2603..>2924)
                     /locus_tag="VFN58_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916655.1"
                     /translation="VISGLFTDAPFWRRIEQPDGSPRSGLTGYQKNIFIRIYPKFASP
                     NGAETIPLAKGECFTRTSPPLIVHKQTCSAFPRETARLQCENSKIPHAAFFIPMDFLW
                     RLCN"
BASE COUNT          607 a          796 c          861 g          660 t
ORIGIN      
        1 gcaatagtca tgtatgtgtt gagcctggac gactcgatgg ttccgcgctg agaaaggggg
       61 tctcggtttc aaaaatgcat cggttgagat atgcaataga aaatgggtac ggtattgcgg
      121 caaagacgct ggatgttgca attcccgccg gaaacattgg ttgctgtcct actcggtgcg
      181 ttccttttca tcccgtcaat tctcagtaac gccgcagagc aaaagaatgc tgaagcgact
      241 aagaccccac cgctgacaac aatcccggtc gaagagatcg cgacgcaggc cacgcaggtc
      301 ggggatctga tccgcggctt cgctgcaaat gttgtcccga ccggcgaaat cgaaacgata
      361 cggaagtttc ttccccaggt gagcgtcgat atagccctgg agctcaaaaa tactaccaac
      421 atcctgcaag agcaggcttc gcttgagacg ctccaggcgc aggaagagat ctggcagcag
      481 agacagtcgc agggcacctc atggctaaaa gtgcttaccg accgggccac gaagctgcag
      541 gttgcgttga cccaactgaa aaaactgcac gagacgtggg ccaagacccg cgatgctgag
      601 gaatccgcaa aggcgccgcc tccgatcctg cagcagatcg atactacgct cgccgttatt
      661 gaagcggcgc aacaaccgca tcaagcgcag cgcgacgaaa ttctcaacct ccagagtaaa
      721 gttgccgaac tagtggccag ttgcaacacg gcgctcgcgc aaatcgcggg gctgcaacac
      781 atggccgttg gggggatctt cacaccggag aacccgccca tttggaacgg cgacctatgg
      841 gctcgcgcca agattacgct gccggagcgt gtcccaaaaa tcgccgcttc ctattggggg
      901 gatattctgc tgtatgtccg agatccctcc cggcacctgc cgtggcacgt tgccatcttc
      961 atagcgctgt cgcttgtgtt gttggcggcg cgccggcagg tcggtcaggg ggacgcggac
     1021 ggagaccgtt cctcgcatgc taccgcagtg ttcgatcatc ctttcgccgc ggcgttgctt
     1081 gtgacattga tgatcgccac gtcacccgct tcaccggtcc cggtcacagt gaagcggctt
     1141 ttccaaatcg cagcgcttgt gccaataatt gtgctcaccc ggcgcatggt gactcctatc
     1201 gtcgttcccg tgatctatgc gctgggaatt ttgttcgcca cggatactgt gcggcgggca
     1261 gtcgtcggtg agccccctat gggtcagggg atactcgcgc tagaggccgt cgcagggatt
     1321 gttgtgttgg gttggttgtg ggtctacgga gcccgtagag cagcgcgagg tctcggccaa
     1381 acgagcactc tgcggcggct ggtggctgct tttattctat tctccctggc catcggattt
     1441 atagccgccg ttttcggcta tatgcgtttg gcacgcctca cgataccggg cgttttcgcc
     1501 gggggtgcgt tggcactgga gctatacgcg tcggctcagg tggccatggg actgatcgtg
     1561 tacgctttac gcttgtggcc gctgcggctt ctccatatgg tccagcatca ccgtgatctg
     1621 atggagcggc gaatctaccg gttgttgtta tggctcgtag ctggcggttg gttactccgt
     1681 tatttggatt atctcgggct cctcgatccg gcatggtctc tggccacagc ctttctcaac
     1741 accagattgg aacgaggctc gatcagcacc tccgtgggcg acatcgtcgc ctttttcctc
     1801 accgtgttgg ccgcatacct gctgtcggct ttcatccgat tcgtgctggc ggaagatgtg
     1861 tattcacgga caggaattgc gacaggccag tcttatgccg tctcgagcct gcttaattat
     1921 gctatccttg ccatcggctt cgtcgtggcc ttgggcgtac tgggaatgga cttgaccaag
     1981 gtaaccgtcc tggccggtgc attcggcgtc ggtatcggct tcggcttgca gagtgtagtc
     2041 aacaatttcg tttccggtct gatcttgctc ttcgagcgcc cgatccatgt gggcgacacg
     2101 gttcaagtag gcagccttca aggcagggtg cgccggatcg gcatccgtgc cagcatcgta
     2161 cgcactatgc aaggcgcgga gatcattgtc cccaacgcgc aactgatcac ggagcaggtg
     2221 acaaactgga cactcagtga ccaactacgg cgcctcgact tgccggtggg cgtgaattat
     2281 ggtgctgcgc caaaaaaagt aatcgagctc ctcgaagccg tggggcgcgc ccatcccaaa
     2341 gtgttgcaag aaccggcgcc ccgtggtctt ttcatgagct atggagacag ttcgatcaac
     2401 ttcgagctgc gagcgtggac ggaacaaacc aactcggtgc aagtccacag tgatttaacc
     2461 gtggccatct acgatgcgat ttatgcggcg gggatgagct ttccttttcc ccagcgcgag
     2521 gtgcgggtat tgtccgacca cggcggagaa tcgaccatga cgcccgtgag agcggccgat
     2581 aaaaagacat agacggagca ggtcaattgc aaagccgcca aagaaaatcc atggggatga
     2641 agaaagccgc gtggggaatt ttgctgtttt cacattgtag tctcgcagtt tcacgaggga
     2701 aggcggaaca agtttgtttg tgcacaatta agggcggaga tgtgcgagta aagcattccc
     2761 ctttggcaag cgggatggtt tccgctccat tcgggctggc aaatttggga taaattctga
     2821 taaaaatgtt tttttgatac cccgtcagcc cgctgcgggg agatccatca ggctgttcaa
     2881 ttctgcgcca aaaaggagcg tcagtgaaga gccccgaaat cacg
//
LOCUS       DASSWZ010000055         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_779886, whole genome shotgun sequence.
ACCESSION   DASSWZ010000055 DASSWZ010000000
VERSION     DASSWZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779886"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1117
                     /locus_tag="VFN58_01320"
     CDS             56..1117
                     /locus_tag="VFN58_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HET8916656.1"
                     /translation="MSLDLTKNSAYVDKTPYEVWQETEGVPIYKGLAIDDLTTVGLGP
                     WKRKGTFGAFINLMGAGRSCDCYACEIQPQGQTLPERYLFEELIYVVKGRGATSVWTE
                     GGPKQTFEWQEGSMFSPPLNTWRQHFNVQRDEPARFVALTDAPVMINRFRNLDFIFDN
                     SFGFRDRFSGEDGYFSGKGRVVEGHRTWDSNFIADVPGFKLIDHSARGQGARGILLHF
                     SANTISAHIEEYPVGTYPRAHWHGPGAHIVILSGEGYSLLWESGKPRTRIDWRPGTIF
                     VPPAKWFHQHFNPGKEPARYLALKPWGFTYQVEDLVKTLEVEAAGGTQIDYKDQDPEI
                     HQTFIQECAKRGVEVQLKF"
     gene            1152..1913
                     /locus_tag="VFN58_01325"
     CDS             1152..1913
                     /locus_tag="VFN58_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET8916657.1"
                     /translation="MNVKMNGEEAKTYVDGIYRQVADRWQQRVNSSQFMKALLAGKLS
                     KETFRLFFKNWAAYTIEINTLEAATYHKHIHFFRKHRDLMAAMAEKLADELMHPKPPG
                     HIHVVMETAKALGISEDEVFVSPMLAEFRAKIDYFRAIVWEGTVAEFYAAGATEEQFG
                     HWAGEVFTALTTHYGLTPEQAVYFSLHEEADTREHEGGIMGHGAFRKMVLRRLLEDGA
                     EVRPGYTLEYCALSCIDLHGAILDACTTAVARCQR"
     gene            1963..3018
                     /locus_tag="VFN58_01330"
     CDS             1963..3018
                     /locus_tag="VFN58_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HET8916658.1"
                     /translation="MDLTKTAAYSNKTPYDLWQESEEIPIVRGHCVEDLTAIPVAPWK
                     RTGARGSFINLVGSGRTCGGYVLEIPPRSETQPQRYLFEQLIYVVKGRGATSVWNQRS
                     TKQTFEWQEGSLFSPPLNAWHQHFNAQGTETARFVALTDAPQMINRFRNLDFIFSNSF
                     EFRDRFNGEEGYYNGKGREVASHRTWESNLVADVRDFGLRDRSLRGQGATGIRLHLAA
                     NTMSAHLEQYPSGTYPRGHRHDPGAHIVILSGEGYSFLWEERRPRIRIDWRPNSLFVP
                     PANWFHQHFNPSAEPVRYLALKPWGFTYKVEDLSKTDQDIKAGGTQIEYKDQDPKIHA
                     IFLAECKQRNTQAKVSI"
     gene            3062..3811
                     /locus_tag="VFN58_01335"
     CDS             3062..3811
                     /locus_tag="VFN58_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET8916659.1"
                     /translation="MNKSEAKTFVDEFYKKVAARWKERVTQGPFMKHLLAGTLPRAAL
                     RLFFKNWGSYTIEINTLEAASYHKHIHFFRSHRDLMAPMAQKLADELVHPEPPGHVHV
                     VLKTAAALGIAEEEIFLSPMLAEFRAKIDFKRSILWEGTVAEFYAAGATEEQTGYWSA
                     DCFKALTTHYGLTAQQAIYFSTHEEADLKEHADGVMGHGSFRRAVLQRLLEEEIATVR
                     PGYTLEYCGMTAVDLHGKILQAAYDAALAAC"
BASE COUNT          999 a         1012 c         1138 g          902 t
ORIGIN      
        1 gatggagcac ggtctagata gcgacttaga aaactcaccg aagtgaggga tgaagatgag
       61 tttggatctc accaagaatt ccgcctatgt tgataagacg ccttatgaag tatggcaaga
      121 aaccgaaggc gttccgatct acaaggggct tgccatcgac gatttgacca ccgtcgggct
      181 gggaccatgg aaacgtaagg gaactttcgg tgcctttatc aacctgatgg gcgccggccg
      241 cagttgtgat tgttatgcgt gtgaaattca gccgcagggc cagacgctgc cggagcgcta
      301 tcttttcgaa gagttgattt atgtggtcaa gggacggggt gcgaccagcg tttggaccga
      361 aggcggccct aagcagacgt tcgaatggca agaaggcagt atgttctccc caccgctcaa
      421 cacctggcgc cagcatttca acgttcaacg agatgagccg gccagatttg tcgccctgac
      481 ggatgcgccc gttatgatca atcggtttcg taatctggat tttatttttg acaactcttt
      541 tggatttcgc gatcggttca gtggcgagga cggatatttc agcggcaaag gcagagtagt
      601 cgaaggtcat cgcacgtggg attcgaattt tatcgccgat gtcccaggat tcaagctgat
      661 cgatcacagc gcacggggac agggagcaag gggaatcctt ttgcacttct cggcaaatac
      721 gatcagtgcg cacattgaag agtatccagt agggacctat ccgcgggctc attggcatgg
      781 gccgggcgcg cacatcgtga ttctttccgg agaagggtat tcactgttat gggagtccgg
      841 aaagccgcgc actcgcatcg actggcggcc gggaacgatt ttcgtgccgc cggcaaaatg
      901 gtttcatcaa cacttcaatc caggaaaaga gcctgcccgc tatctcgctc tgaaaccatg
      961 ggggttcacc tatcaggttg aagatcttgt gaagaccctg gaagtcgaag ccgccggcgg
     1021 aacacagatc gactacaagg atcaagaccc cgaaattcat caaaccttta tccaagaatg
     1081 cgcgaagcga ggggtcgagg ttcagctaaa attttgagtg gacaacaact aagaatcacg
     1141 aggaagccgc gatgaatgtg aagatgaacg gagaggaagc caaaacgtac gtcgatggga
     1201 tttataggca agtcgccgat cgctggcagc aaagggtcaa cagctcgcaa tttatgaagg
     1261 ctctgctcgc gggaaaatta tctaaagaaa ccttccggct cttttttaaa aactgggccg
     1321 cgtacaccat cgagatcaat actctagaag ccgctacgta tcataaacac atccacttct
     1381 ttcgtaaaca tcgcgacttg atggcggcga tggcggaaaa gttggcggat gagctaatgc
     1441 atcctaagcc gcccggccat attcacgtgg ttatggaaac ggccaaagcg cttggtatca
     1501 gcgaggatga agtttttgtt tctcccatgc tcgccgagtt ccgtgccaaa atcgattact
     1561 tccgcgccat tgtttgggaa ggcacggtgg cagagttcta tgctgccggc gccaccgagg
     1621 agcagttcgg tcactgggcg ggcgaagtct tcaccgcttt aacgactcat tacgggctga
     1681 ccccggagca ggcggtgtat ttttcgcttc atgaggaagc tgacacgaga gagcatgagg
     1741 gcggcatcat gggacatggg gcattccgaa aaatggtgtt gcggcgtctc ttggaagacg
     1801 gcgcggaggt gaggccgggc tatacgctgg aatattgcgc cttgagctgc atcgatttac
     1861 acggcgcaat tcttgatgct tgcacgaccg cggtcgccag atgccagcgg tgagtttagg
     1921 tgtgatcctt ttgggaagaa tcctcgcggg attcatactt gcatggattt gacaaaaaca
     1981 gcagcctact ccaataagac accctacgat ctgtggcagg agtcggagga aattccgatt
     2041 gtgcgtggtc attgcgtaga agatctgacc gcgattccgg tcgcaccctg gaagcgcacc
     2101 ggtgctcgcg gatcgtttat caatctcgta gggtcggggc gtacctgtgg cggctatgta
     2161 cttgagattc ctccgcggag cgaaacccag ccgcagcgct atctttttga gcagttgatt
     2221 tatgttgtca aaggtcgcgg cgccaccagt gtttggaatc aacgatccac caaacagacc
     2281 tttgagtggc aggagggaag ccttttttcg ccgcccctaa acgcttggca ccagcacttc
     2341 aatgcacagg gaaccgagac cgcccgtttt gtcgcgctca ctgacgcgcc ccagatgatc
     2401 aaccgcttcc gaaatctcga cttcattttc agcaacagct ttgaattccg ggacagattc
     2461 aatggtgagg aaggctatta caacggaaag ggaagagaag tcgcgagcca ccggacgtgg
     2521 gagtccaatc ttgtcgcaga cgtgagagac tttggtctca gagatcgaag cttgagaggt
     2581 cagggagcga cgggaatccg gctccacctt gccgccaata cgatgagcgc tcatctcgaa
     2641 cagtatccgt ccggtactta tccgagaggt catcgacacg accccggggc gcatatcgtg
     2701 atactttcgg gcgaaggcta ttcttttcta tgggaggaaa ggcgccctcg catccgaatc
     2761 gattggcgtc cgaatagtct gttcgtgcca ccggctaatt ggtttcatca gcattttaat
     2821 ccctccgcag aaccggtccg ctatctcgct ttgaagcctt ggggtttcac ctacaaggtc
     2881 gaagatctct cgaagaccga tcaggacatc aaggcgggag ggacgcagat agagtacaaa
     2941 gatcaggacc ctaagattca cgccatcttt ctggcggagt gcaaacaaag aaacacccaa
     3001 gcgaaggttt caatctgaac cggcgtcgcc gacaatagat taagaattga tcgaggaatt
     3061 tatgaacaag agcgaggcaa agaccttcgt cgatgagttc tataagaagg tcgccgccag
     3121 gtggaaagaa agagtcacgc aaggaccctt catgaaacac ctgctggccg gaaccctgcc
     3181 gcgcgcggca ttgcggcttt tctttaaaaa ttgggggtcc tatacgattg agatcaatac
     3241 cctagaagcc gcttcctatc acaagcacat ccacttcttt agaagccatc gcgatctcat
     3301 ggccccgatg gcgcaaaagc ttgccgacga acttgtccac cccgagcctc cggggcacgt
     3361 ccatgtcgta ctgaaaaccg ccgcggcgtt gggtatcgct gaagaagaga ttttcctttc
     3421 gccgatgctc gccgagtttc gcgccaagat tgattttaaa agatcgatcc tgtgggaagg
     3481 tacagttgca gagttttatg cagcgggcgc aaccgaggaa cagacgggtt actggtcggc
     3541 ggattgtttc aaagccttga ccacacacta cggtctcact gcacagcagg cgatttattt
     3601 ttcaactcac gaggaagccg acctcaaaga gcacgcggat ggtgttatgg gccatgggag
     3661 ctttagacga gcagtcttgc agcggctttt ggaagaggag atagcgactg ttcgacccgg
     3721 ctacacactc gaatattgtg gaatgacggc ggttgatttg cacggaaaga ttctccaggc
     3781 cgcctatgat gccgccttgg cagcgtgcta ggcgcaacag cactctcggt tttcgatcgg
     3841 cgatgagatt tgttcgccca cgctggcaaa gcgactaccc gccaggtgtt ttatggagaa
     3901 gcatcccgat acaccggcaa gtttgccaaa tcaaatcaca attgacgcgg tccaacctat
     3961 tggagactga taaggagtcc acatgttgtt gttgaaagat gaagacgtgc agaaagtgct
     4021 gactatgccc atgacgctgg aggcgctcga c
//
LOCUS       DASSWZ010000056         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1303592, whole genome shotgun sequence.
ACCESSION   DASSWZ010000056 DASSWZ010000000
VERSION     DASSWZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303592"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFN58_01340"
     CDS             complement(<1..387)
                     /locus_tag="VFN58_01340"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HET8916660.1"
                     /translation="MNKVALADIVGNAAYEKMREKSRQRIIELKRHRRVSVGDKVTLV
                     FENRDTVIFQIQEMMRAEKISDLDKIREEIEVYNELIPAPGELSATLFLEIEDQTHLR
                     EDLLKFLGIDESVYLKVGNHSIHARFE"
     gene            complement(451..900)
                     /locus_tag="VFN58_01345"
     CDS             complement(451..900)
                     /locus_tag="VFN58_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HET8916661.1"
                     /translation="MAKSLKGSKSHGNLKNAFAAESQANRRYLYFARVADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATDVPFGSTEKNLKSAVEGETYEYTEMYPGF
                     AKTAREEGYTELAEWFETLAKAEKSHAGRFSKGLEKIAGREPADAAS"
     gene            complement(991..1701)
                     /locus_tag="VFN58_01350"
     CDS             complement(991..1701)
                     /locus_tag="VFN58_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916662.1"
                     /translation="MARARYASTVVLLRPDEDGSFEILLTRRPPEMQFLGGFYVFPGG
                     AVHKDDYSTKALERCRGLSANDAQKILGNRHDPELALGHWVACVRELFEEVGVLLCES
                     RSGGPIELLDEMTKAKFETKRQAIVRGKLGFGEFLESEELWCDLSRIIYFFHRVTPEF
                     YPMRFDTRFYLASLPAHQTALSRSEEVTHAVWIKPAEALAEVYHNDFPILPPTTTVLE
                     DLAGIHTWEELRARYRLC"
     gene            complement(1714..2757)
                     /locus_tag="VFN58_01355"
     CDS             complement(1714..2757)
                     /locus_tag="VFN58_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916663.1"
                     /translation="MKLFASALLVMVSWQSQVCAQGKPATVADLAAYNKPDREQVLYE
                     GAKKEGKLMWYTSLTGGPNTEAPKVFEAKYPGVKMEVYRGDSDALISRILQEAQAKRY
                     LVDTIETTFPILKVMQDEKIIARYSSPYLASYPDEVKEKAAKGLVYWATDRETYIGVA
                     YNTNVVPAGAAPKRFEDLLKPELKGKIGFATSDTGNRVIGAMLATRGGEFVQKLRSQD
                     ITLHGVSGRAILDMVISGELGVSPTVFLSHSRVSISKGAPIRWVPMDVVPTNAGGVAL
                     PAQAPHPHAALLFADFLLSPEGQKFLGKFGLDSAMNKPDFKRWYAEAGMTVEQYEKEN
                     SKWEKLLREIGRK"
     gene            complement(2784..>3224)
                     /locus_tag="VFN58_01360"
     CDS             complement(2784..>3224)
                     /locus_tag="VFN58_01360"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8916664.1"
                     /translation="LILAHEGTLERFTGDGMMIFFNDPVPVANPAERAIRMALAMRES
                     VAELTVKWRKLGYELDFGVGIAQGYATIGGIGFEGRWDYGAIGTVTNLASRLCGEAKP
                     SQILVSQRLLGTVEELVEVEPVGELTLKGFHRPITAHNVLRLKN"
BASE COUNT          682 a          952 c          817 g          773 t
ORIGIN      
        1 ttcaaagcgc gcatggatgg aatggtttcc gaccttaagg taaaccgatt cgtcgatgcc
       61 caagaatttc agcaaatcct cgcgcagatg ggtctgatcc tcgatctcca aaaagagggt
      121 ggcgctcagt tctccgggcg cggggattag ctcattgtag acctcaattt cctccctaat
      181 cttatccagg tcggagattt tctccgcccg catcatctct tggatttgaa agatgaccgt
      241 gtctcggttc tcgaagacca aggtcacttt gtctcccaca gaaacgcgtc ggtgccgctt
      301 aagctcaata atgcgctggc gcgacttctc gcgcattttt tcataggcgg cattgcctac
      361 gatatccgct agtgcaacct tgttcataac ttgccgtctt ccactgtggt cagggattgc
      421 gattgccatc cctgaccttc tacagatcaa ttagcttgcg gcgtccgcgg gctctctacc
      481 ggcaatcttc tccaagcctt tggagaatcg gccggcatga gatttttccg ccttggccaa
      541 ggtttcaaac cattcggcca attccgtgta tccctcctca cgtgctgtct tggcaaaccc
      601 gggatacatc tcggtgtatt cgtaagtctc cccctcgacg gcagatttga gattcttctc
      661 tgtgctgccg aagggaacgt cggtggcggg gtcgccgact tctttcaaaa aatccagatg
      721 gccgaaggca tgtcccgtct cagcctcgga cgtgtcccgg aacaaccctc cgacatccgg
      781 atagccttcg atatctgcca cccttgcgaa atagagataa cgccggttag cctgagactc
      841 ggccgcgaag gcgttcttca gattcccatg gctcttagag ccctttaaac tttttgccat
      901 tatgctgtcc tccattaagg ttattaagta gagagtgcgt agactgtcaa ggggtggaga
      961 tgaattcttt gtttcaggga gccggccagc tcagcagagg cggtatctag cgcgtagctc
     1021 ttcccaagta tgaattcccg caagatcctc taataccgtc gttgtcggag ggaggatggg
     1081 gaagtcgttg tgataaacct ccgccaacgc ttcggccggc ttgatccaca cggcgtgtgt
     1141 tacctcttcg gaccgcgaca gcgccgtttg atgcgccggc agcgaagcca ggtaaaaacg
     1201 cgtgtcgaat cgcatcggat agaattcggg cgtcacgcga tggaagaaat agatgatacg
     1261 ggacaggtca caccagagtt cttccgattc gaggaattct ccgaagccaa gttttcctct
     1321 cacgattgcc tgacgcttgg tctcgaactt tgctttcgtc atttcgtcga gcagttcgat
     1381 aggcccaccc gatctcgact cacaaagcag tacgccaacc tcttcaaaca attctcgaac
     1441 acaggcgacc caatgcccta gggcaagttc aggatcatgc cggttaccga gaattttctg
     1501 cgcgtcgttc gcggataatc cccggcagcg ctccagcgcc tttgtcgaat agtcgtcttt
     1561 gtggacggcc cctcccggaa aaacgtagaa gccgcccaga aattgcatct caggcggcct
     1621 acgagtcagc aggatctcga agctgccgtc ctcatccggc cgcaggagaa cgaccgtgga
     1681 agcataacgc gctcgcgcca taaacaggag aaattacttt cgcccaatct cccgcaacag
     1741 tttctcccat ttgctgtttt ccttttcata ctgctcgacg gtcatgcccg cctcggcgta
     1801 ccaacgcttg aaatcaggct tgttcatagc actatcaagg ccgaacttgc ctagaaactt
     1861 ctgcccctca gggctgagca ggaagtcggc gaacaaaaga gcagcgtgcg gatggggcgc
     1921 ttgcgcaggc agcgcgacgc cgcccgcgtt ggtgggcaca acgtccatgg gcacccacct
     1981 gatcggcgct cccttcgaaa ttgatacccg cgagtggctg agaaaaacag tgggagatac
     2041 gcccaactct ccggaaatca ccatgtccag tatcgccctt ccggaaacgc cgtgaagggt
     2101 tatatcctgt gatctgagct tctggacaaa ctcgcctccc ctggtggcca acatcgcacc
     2161 gatcacacgg tttcccgtgt cgctcgtggc gaatccgatc tttcctttaa gctcgggctt
     2221 tagcaggtct tcgaatcgct ttggcgccgc gccagccgga accacattgg tgttgtaagc
     2281 gaccccgata taggtttcac ggtccgttgc ccagtaaacg agtccctttg cggctttttc
     2341 tttgacctcg tcgggatagg aggccagata aggtgaggag taacgggcaa tgattttttc
     2401 gtcctgcata acttttaaga tcgggaaggt ggtttcaata gtatccacca gataacgctt
     2461 cgcttgcgcc tcctgaagta tcctcgaaat cagagcgtcg ctgtcacccc ggtaaacttc
     2521 catcttgacc cccgggtact tggcctcgaa gaccttcggc gcctcagtat tcggtccacc
     2581 ggtaagcgag gtgtaccaca tcaatttgcc ttccttcttc gccccttcgt aaagaacctg
     2641 ctcgcgatcc ggtttgttgt acgccgcaag atcggcgact gttgccggtt ttccctgagc
     2701 gcacacctgt gactgccagc tcaccataac gaggagcgcg gatgcgaaaa gtttcattct
     2761 ttcttcccct tgcttttccg atcttagttc ttcagcctta agacgttgtg cgcggtgatc
     2821 gggcggtgaa agcctttgag cgttagctcg cctacgggct ccacctccac caactcttcc
     2881 acagtcccca gcaggcgttg agaaactaaa atctggctgg gtttggcctc gccgcagagt
     2941 cgcgaggcaa gattagtgac cgtcccgatg gcgccgtaat cccagcgtcc ttcaaaaccg
     3001 atgccgccga tagtcgcata gccctgggca atgccgacgc cgaaatctag ctcatagccc
     3061 aacttccgcc acttcaccgt cagctccgcc acactctcgc gcattgcaag cgccatacga
     3121 attgcccgtt cggctggatt ggccacgggc accggatcat tgaagaagat catcatgccg
     3181 tcgccggtga atcgttccag agtcccttcg tgcgccaaga tcag
//
LOCUS       DASSWZ010000057         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1006384, whole genome shotgun sequence.
ACCESSION   DASSWZ010000057 DASSWZ010000000
VERSION     DASSWZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006384"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            645..1562
                     /locus_tag="VFN58_01365"
     CDS             645..1562
                     /locus_tag="VFN58_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916665.1"
                     /translation="MSSNLLQTFVRLSLIIAVFSGGGCSRTVNPEIRYVPAGDLLDIV
                     KEFQRLAREDTYRFPIPKDVTGVNIMKATLVRLDDYEKKNPGKFTDIIQFNKALALER
                     LREYEQAAALYRKVAETEGRLGKEASKNAEILDTFLLILDRPLASDDPFEYIKGLDEK
                     VIAWNQLISKHQGTAYEFLARVEEERVDRAKVAFVEANRYRLKEGNQLAILGYTQLST
                     KHRQSKNIYRFLIDFGEFYELLAKEYASQYDPEGLNFDPKSFELFVKSALRLYNEVAQ
                     VDGILEKIEAQGKIEALRGLHEKITRLSR"
     gene            1559..>3300
                     /locus_tag="VFN58_01370"
     CDS             1559..>3300
                     /locus_tag="VFN58_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916666.1"
                     /translation="MMTKLILTSLLLLILGSTHVWPEPCAAQELAIEKILNPMPDYDP
                     FDKSTTGAPQFFPDAVDKRTRELLIDALTNQQESLKDHLQFLKEQDSQLQKEYNTSTG
                     LTERALDLTNNTIQDRQRYLAAQEKALKNASTPERKKYLQSIINNDDLNQADDLMRQS
                     STNQWGGIVNRMLSSVDLAGVASGNYIGAAAETVISQLYALADRDMAIEERRALARHL
                     DHLKRFPNDPRNAEILRQVEGIEGKKKSVLIKKQLARGDEALGKGELERALFYYETAF
                     FIDPESKSTERALQKATKLYQEQQEIRNKGLASGKEGSFSSDQEQDVEQLLNALSLRD
                     PALIERQSIDVGKKHHGKPLGDAALDTEAVALELRGQHEAAKRMIDQLARSSKNPDLQ
                     RRAAALLGSPEYNLLGSFQDARNDRNIESVKYVLLGEDLLKKNLIYAAGAMAAAGPAG
                     GVTLGAINAMMLGNNLVKVISNNPISAQPVIDAGVAYIRNHPKSENASEVYTVLADAY
                     AERGMFDKAVNFHQLAGAPPEQITALKEKAASAMLNAATKSKGRGTQQYYLTTIIDEY
                     PESPAAAEATKKLAA"
BASE COUNT          875 a          909 c          874 g          642 t
ORIGIN      
        1 cgcgtcggcg gcccggcaac caccggaagg ggtggcggcg gagggacagg cgctggagca
       61 gaagaaacag ctggcgcagg tggagcgacc tcaggctcag gactatcgga agtggtagcg
      121 cagccaaaca gggcgagtcc cagagtaagc atgattacaa cgaatccagg ccctactccc
      181 ctctgaattc gatgaaccag ccgcaacacc gggaacttca tctcttgacc ttcctgtaag
      241 tttaacttgc tccgggttgc aaacgcaata acaaattcaa atcggtacca tgaggccggc
      301 acagcgtggt ggccacggag acaacgattt atctgctcag tccgtaagct cttcgcttag
      361 agatcagcag gcgctttttg cggcggagcc aaaattgccc ttgactttgt ctgaaaactt
      421 ctgaaactat gcaggcaata agggaagttg tctaatgcta tccctttcaa cacggatctg
      481 tgttgctgca aaccgtcggc aagtccagtt ggcccatcca ttccctagtg agaacaacgt
      541 tccccggaaa ggcgcattat cggataaaca acggacagaa tagctcacca ctcaagattg
      601 ggatcgtcct ctacatcggg tgcccgcgaa atcaagagat cattttgtcg tcaaatttac
      661 ttcagacttt tgttcgcctt tcactcatca ttgcggtttt ttcgggcgga ggctgttctc
      721 ggaccgtcaa ccccgagatt cgctatgtcc cggccgggga cctgctggat atcgtaaaag
      781 aattccagcg cctggcgcga gaagacacct atcgattccc tattcccaaa gacgtcaccg
      841 gcgtcaacat tatgaaggca accctggtgc gactcgacga ctacgaaaaa aagaacccgg
      901 gaaaattcac cgatatcatt caattcaata aagcgctggc cctggagcgg ctccgagaat
      961 atgagcaggc ggcggccctc tatcggaagg tcgccgaaac cgaaggccgt ttaggcaagg
     1021 aagcatcgaa gaatgccgaa attctcgata cgtttctcct gatcctggat cgaccgctgg
     1081 ccagcgacga cccttttgaa tatatcaagg gtctcgacga gaaggtcatc gcttggaacc
     1141 agctgatcag caagcaccag ggaaccgcct acgagtttct cgcccgcgtg gaggaagaac
     1201 gagtcgatcg cgctaaggtc gccttcgtcg aggccaaccg ataccgcctc aaggaaggca
     1261 accagctggc gattctgggc tatacccagc tatcgaccaa acaccggcaa agtaaaaata
     1321 tctatcgttt tttgattgac tttggcgagt tctacgagct acttgcaaaa gaatacgcgt
     1381 ctcagtacga tccggaaggt ctgaactttg accccaagtc tttcgagctg ttcgtcaagt
     1441 cagccctcag gctctataat gaagttgccc aggtggatgg aatccttgaa aagatcgagg
     1501 ctcaaggtaa gattgaagcc ttgcgcgggc tgcacgagaa aataacgagg ttaagccgat
     1561 gatgacaaaa ctgatcctaa cctctttact cctgctcatc cttggcagca ctcacgtgtg
     1621 gcccgagccg tgtgctgccc aagaactagc aatcgaaaaa attctcaacc caatgccgga
     1681 ttacgatccc tttgataaat ccacaaccgg agctccgcag ttctttcccg acgcggtgga
     1741 caaaagaact cgcgaactgc tgatcgatgc gttgaccaat caacaggagt cgctcaagga
     1801 tcacctgcag tttcttaagg aacaagacag ccagttgcag aaggaataca acacatcgac
     1861 cggtttgacc gaacgcgcgc tggacctgac taataatacg attcaagacc ggcaacgcta
     1921 tcttgccgcc caggaaaagg ccctgaagaa tgcctcgacg ccggagcgaa agaagtatct
     1981 tcagtcgatc atcaacaacg atgacttgaa ccaggcggat gatttgatgc ggcaaagctc
     2041 gacgaatcaa tggggcggca tcgtcaatcg gatgttgagc tcagtggatc tggccggggt
     2101 cgcgtcgggc aactacatcg gcgccgcggc agaaactgtc attagccaac tctatgccct
     2161 tgccgaccgc gacatggcca tcgaggagcg ccgcgcgcta gcgcgccatc tcgatcatct
     2221 caaacgtttt cccaacgatc cgcgcaacgc tgagatcctc aggcaagtgg aagggatcga
     2281 gggcaagaag aaaagcgtat tgatcaagaa gcaactcgcc agaggcgatg aagctctggg
     2341 caagggcgag ctggagcgag cccttttcta ctatgaaacg gcatttttca tcgatcccga
     2401 atcgaagagc accgagaggg ctcttcaaaa agccaccaag ctctatcagg agcaacaaga
     2461 gataagaaac aaggggctcg catccggcaa ggaagggtct ttctccagcg atcaagagca
     2521 ggatgtagaa caactgctga acgccctcag tctgcgtgat ccggctctca ttgagcgcca
     2581 gtccatcgat gtcgggaaaa aacatcacgg aaaaccgctt ggcgatgcgg ccctggacac
     2641 agaagccgtc gccctggaac tacggggaca gcacgaagca gccaaaagaa tgattgatca
     2701 gctcgcccgg tcgtcgaaga accctgatct ccaaaggcgc gcagccgcgt tgttggggag
     2761 cccggaatac aatctgctcg gcagcttcca agacgcccgc aacgaccgca acatcgaatc
     2821 ggtgaagtat gttttgctcg gtgaagacct gctcaagaaa aacctgatct atgccgctgg
     2881 cgccatggcc gccgccggtc cggcgggcgg ggtcaccctc ggggcgatta acgccatgat
     2941 gctaggaaac aaccttgtca aggttatttc caacaacccg atttccgccc aaccggtgat
     3001 cgacgcaggt gttgcctata tccgcaacca tccgaaatcg gaaaacgctt cggaagtgta
     3061 caccgttctc gcggatgcct acgcggagcg gggcatgttt gataaagcgg tgaacttcca
     3121 ccagttggcc ggcgcgccac cggagcaaat cacggcgcta aaagaaaagg cggcgagtgc
     3181 catgctcaat gctgccacaa aaagtaaagg ccggggcacc cagcagtatt atttgacgac
     3241 catcatcgac gaatatccgg aaagtcctgc ggcagcggaa gccactaaga aactcgcggc
//
LOCUS       DASSWZ010000058         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_426377, whole genome shotgun sequence.
ACCESSION   DASSWZ010000058 DASSWZ010000000
VERSION     DASSWZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426377"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFN58_01375"
     CDS             <1..369
                     /locus_tag="VFN58_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HET8916667.1"
                     /translation="KADPALWTREIFARIFKHCLPKSAELYTYSASTAVRVSLLTAAF
                     FVAEGVGTGPKSDTTIAFTRAAGARTHPLSPQLLGQKWLTRWRRSGSKFPTTLPTEDR
                     PHFEKLIETHRQFLSDSFPD"
     gene            complement(435..1421)
                     /locus_tag="VFN58_01380"
     CDS             complement(435..1421)
                     /locus_tag="VFN58_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916668.1"
                     /translation="MQRKMHRVMVLLLALGISMLHASAPAIAADKPDKLRIAYITTSG
                     SMATLWMAAASSAFQNEGLDVELVYIGASAAMAAILAGEVDAVEISAPGIVPVVLAGG
                     NLTMIAGLLNKMIYSLHAQKEIKSAAQLRGKVVGTDRVGTPGNYGVRTTLTKLGLNPD
                     TDVQLLSVGGLDIRWPALQSRQIAAAGLTPPATFRADAQGFTKLTDTYDLPYQNVGLI
                     VRKNEVEKRSDVWLRLLRAVRRGIDVWYENPQLAKRVLARYTKENDSAMLEKTYEFFT
                     KQAGFNRDLTFTDRGFEQILKFLGATVLPAAKDAPVNQFYDTRIIERLKKGS"
     gene            complement(1483..2613)
                     /locus_tag="VFN58_01385"
     CDS             complement(1483..2613)
                     /locus_tag="VFN58_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916669.1"
                     /translation="MEKPIISADGHLDLFYLPPDTFTSRTSSRLKDKVPKVVEVDGIP
                     SWVGNGAIMGKYRGWMGRGELTGHRGKRMMEVGWEPTHPADPVLRVKDLDIDGVQAEV
                     IYGIRFVEDSIKDPEVISATYRAYNDFVAEFCAHNPKRLIGIADIPAHSVEGAVEETR
                     RIGKGGLGLRGALFDWFNGPKPIWHPMWEPLWAAAEEADVVLSFHIGAGHGTTTVGPT
                     GVAEKLSGTIPRVSMAAHQAVVAMQADECLASVILCGALERHPKLKVVLAESHIGWIP
                     YLLDRLDTKFKEGSYKDLLGTMPSELFRRQMWATFQDDKVGARLAEDYAPDNFCWASD
                     YPHLDSVWPDSHAFIRESMEHLSRKMQEKLTYGNVTSLYHLE"
     gene            complement(2679..>3435)
                     /locus_tag="VFN58_01390"
     CDS             complement(2679..>3435)
                     /locus_tag="VFN58_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916670.1"
                     /translation="IQLAQMGGPAALAAGVAGMDLSFVAVALNTTPIVIMGTVSRMED
                     LKGKAIGVTRYGSNTDISARFAIRKAGLQPEKDVALIQLEDYPGIMGGIQSGRIAAGA
                     LADPFTDYAKKLGYKEIADIAALGLEFPFVGIVAKKSYLKDNPDITLRFVRAYTEAIA
                     LYKNNPELAMKVTSKYTGIKDPSILSSTVNFYAPKLARVPYPTLGGMRFVLEQIAIRD
                     PKAKNVNPESFMDVRFVKQLEDSGFIQTLYPKG"
BASE COUNT          768 a         1011 c          855 g          801 t
ORIGIN      
        1 aaagcggacc ccgcgctttg gacccgtgag atctttgcgc gaattttcaa gcattgcctg
       61 ccaaaatcag cagagctcta cacctactcc gcatcgactg ccgtgagagt ttcacttttg
      121 acagcagcgt tttttgtcgc cgaaggcgtc ggcacaggac ccaaatcaga caccaccatc
      181 gctttcacaa gagcggcagg agcccgaacc catcccttgt ctccacagct cctgggccag
      241 aagtggctca cgcgatggag acgaagtggg tcaaaatttc caacgactct accaactgaa
      301 gataggcctc attttgaaaa gctcattgaa actcatcgcc aatttttaag cgactccttc
      361 ccagattagt tgatcaccca gaaggaacct gaggtcttgc aatcctccat cggcgtcatg
      421 caggcacgat accatcacga cccttttttc agtctctcga taatccgggt atcgtaaaac
      481 tggttgaccg gggcatcctt tgccgctggg agcactgtcg ctcccaagaa ttttagaatc
      541 tgttcgaaac cgcgatcggt aaaggtgagg tccctattga aaccggcctg cttggtaaaa
      601 aattcgtagg tcttttccaa catcgccgaa tcgttctctt tggtatatct ggcaaggacg
      661 cgcttggcca actgcgggtt ctcataccac acgtcgatgc cgcgtcgaac ggcgcgcaac
      721 aagcgtagcc aaacgtccga tcttttctcg acttcatttt ttctcacaat caggccgaca
      781 ttctggtaag gcaaatcata cgtatcggtt agcttggtaa atccttgcgc atctgcccta
      841 aaggtcgccg gcggcgtcaa tcccgctgcc gcaatctgtc tcgattggag tgctggccac
      901 cggatgtcca atccaccgac agacaacaat tgcacatcgg tgtctggatt caggccgagc
      961 ttggttaaag tcgttctcac tccgtaattg cccggggtgc ccacgcggtc tgtgccgacg
     1021 accttaccgc gcaactgcgc ggctgatttg atttccttct gtgcgtgaag agaataaatc
     1081 attttattga gcagaccggc tatcattgtc agattcccgc ccgcaagcac caccggaacg
     1141 atacctggag cagaaatctc cacggcatcg acctcgccgg ccaagatcgc cgccattgcg
     1201 gcgctggctc cgatatagac gagttcgacg tcgagccctt cattctggaa agccgaagag
     1261 gctgcggcca tccataaagt tgccatcgac ccactggtag taatgtaagc gatcctaagt
     1321 ttatctggct tgtccgccgc gatcgcaggc gctgacgcgt gcagcatcga tatcccaagc
     1381 gccagaagta aaaccatcac gcggtgcatt ttcctttgca tagctcacgc gccaggacgc
     1441 ttttgagttt cgcatccagg gaatgaagat gacagaccga atttattcaa gatggtatag
     1501 ggaagttaca ttaccgtaag tgagtttctc ctgcatcttg cggctgagat gctccataga
     1561 ctcacggata aatgcatgcg agtcaggcca taccgaatcc aaatgcgggt agtccgaagc
     1621 ccagcagaaa ttgtccggtg cataatcctc cgccaggcgc gcccccacct tatcgtcttg
     1681 aaaagtcgcc cacatttggc gccggaacaa ctcgctcggc attgtcccga gcaaatcctt
     1741 gtaggagcct tccttaaatt tagtatcgag cctatcgagc aaatatggga tccagccgat
     1801 gtgactctcg gctaaaacga ccttaagctt gggatgcctc tccagcgcgc cgcataagat
     1861 cacagaagcc agacactcgt cggcttgcat ggccaccacg gcttgatgag ccgccatgga
     1921 gactcgtggt atggtccccg acaatttctc ggccaccccg gtggggccga ctgtcgtggt
     1981 tccgtgaccg gcgccgatgt gaaaagagag tacgacatca gcttcctccg ccgcagccca
     2041 gagcggctcc cacatcggat gccagatcgg cttcgggccg ttgaaccagt caaagagtgc
     2101 tccccgcaga cccaaacctc cctttcctat tctccgggtc tcctcgactg ctccttccac
     2161 ggagtgcgcc gggatatccg ctatgccgat caaacgttta ggattgtgcg cgcagaactc
     2221 cgcaacaaag tcgttgtagg cccggtacgt cgctgagatc acttccggat ccttgatgct
     2281 atcttcgaca aaacgaatcc cgtatatgac ttccgcttgc acaccatcga tgtctaaatc
     2341 cttgacccgc agtacgggat ccgcgggatg cgttggctcc catccaacct ccatcatcct
     2401 tttgccccgg tggcccgtca gctcaccccg tcccatccaa ccgcgatact ttcccatgat
     2461 cgctccattc ccgacccagg agggtatccc gtcaacctcg acaactttcg ggaccttgtc
     2521 cttcaagcgc gacgaagtac gagacgtgaa agtatcgggg ggaagataga acagatccaa
     2581 atgtccatcc gcggatatga ttggtttttc cattgatata cctcgatcct ttttgttgag
     2641 cgtttattta gacgtgatag ccaatcccgt ttaccaacct atcctttcgg ataaagcgtc
     2701 tgaataaagc cgctgtcttc gagctgtttg acgaagcgca cgtccatgaa cgattccgga
     2761 ttgacgtttt tggccttcgg gtcgcggatc gcgatctgct cgagcacgaa gcgcatccct
     2821 ccgagcgtcg gatacggcac ccgcgccaat tttggggcat agaaatttac cgttgaggag
     2881 agaatcgacg gatctttgat gccggtgtac ttgctcgtga ccttcatcgc cagttcggga
     2941 ttgttcttgt acagcgcgat cgcttcggta taagcgcgga caaagcgcag ggtgatgtcc
     3001 ggattgtcct tgaggtagct tttcttggcg acgatgccga cgaaggggaa ctccagccca
     3061 agggccgcga tgtcggcgat ctctttatag ccgagctttt tagcgtagtc cgtaaacgga
     3121 tcggcgagtg cgcccgcggc gatgcgaccg gactgaattc cccccatgat gcccgggtaa
     3181 tcttcgagtt gaatcagcgc cacatctttt tccggttgca ggccggcctt tctgatcgcg
     3241 aagcgagccg aaatatcggt attggaacca taacgagtga caccgatggc tttccctttg
     3301 agatcctcca tgcgtgatac cgtccccata atgacaatag gcgttgtgtt cagcgctacc
     3361 gctacaaaac tcaagtccat gcccgccacg cccgccgcca gcgccgcagg gccgcccatt
     3421 tgcgccaatt gaatc
//
LOCUS       DASSWZ010000059         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1218741, whole genome shotgun sequence.
ACCESSION   DASSWZ010000059 DASSWZ010000000
VERSION     DASSWZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218741"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /gene="gpmI"
                     /locus_tag="VFN58_01395"
     CDS             complement(<1..1319)
                     /gene="gpmI"
                     /locus_tag="VFN58_01395"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET8916671.1"
                     /translation="MQPVILVILDGWGINPRKEGNAIAQASIPNIDRLTREFPVSSLS
                     MSGVDVGLPEGQMGNSEVGHMILGAGRIVYQDLTLIHQDIDEGSFYRNKNLLDAMRKT
                     RDKSGRLHLMGLLGDGGVHSHQRHMEALIEMARREKVGTVYLHLFLDGRDTPPNSGEQ
                     FMLDLNEKLKAYPEVRIATVSGRYYAMDRDKRWDRTEKAYLCLTEGIGNKADSALEAI
                     RNSYKDKVTDEFVLPTIISGVVPEGLIRDADGVIFFNFRADRARQLTRALTEEDFKEF
                     PRKRRVALSAFATMTQYDETFKVPVAYPPREIKKILGELASEAGIRQLRIAETEKYAH
                     VTYFFNGGEENKFPGEERILIPSPKDVATYDLKPEMSARKVTEALVKQLREQDVGLVI
                     ANYANADMVGHTGNFEAAVQACEVIDECIGKVVDAAMSKKGKMIITA"
     gene            complement(1404..1808)
                     /gene="rsfS"
                     /locus_tag="VFN58_01400"
     CDS             complement(1404..1808)
                     /gene="rsfS"
                     /locus_tag="VFN58_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET8916672.1"
                     /translation="MAAISSWDKVLLLTRFALEKKARDLVVLEVRELTSIADYFIICS
                     GSSDRQVQSIAQGIEENLSEAGHSLLSVEGANRGHWVLMDFSDVIVHIFYEPVREFYD
                     LDGLWGHAPRVELPEPYSTLVDQFQAADHPLS"
     gene            complement(1814..2476)
                     /gene="nadD"
                     /locus_tag="VFN58_01405"
     CDS             complement(1814..2476)
                     /gene="nadD"
                     /locus_tag="VFN58_01405"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET8916673.1"
                     /translation="MRIGLFGGTFDPIHWGHLRSAEEVSETFELDRVVFVPASIPPHK
                     RGQTTTPARDRLAMVRLAVARNPRFKVSTVEIARPGVSYSIDTVRHFAKRKHRGDSLF
                     FIIGLDAFQEIGSWKNFAEIFPLCNFIVTSRPGSKASDPLRGTGVAVKKLFCYDFKQR
                     NYRHGSGSRVLFTRLTDIAISASEIRGLVEEGKSIRYLVPSRVETYIKKQGLYQNRRE
                     ER"
     gene            complement(2667..>3740)
                     /locus_tag="VFN58_01410"
     CDS             complement(2667..>3740)
                     /locus_tag="VFN58_01410"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET8916674.1"
                     /translation="GISEPIVDRIALNPGRVQAMAKGLRDVAALPDPVREVVKMWRRP
                     NGLQVGKMRIPLGVIAIIYEARPNVTADAAALCLKSGNAVILRGGSEAHFSNQAIGQI
                     LRKACAQTRVPREAVQIVQTKDHAFVQELLQLEEYIDLVIPRGGEELIRAVVTHSKVP
                     VIKHYKGVCHVYVDADASHEMAERICMNAKVQRPSVCNAMETLLVHETIAPAFLPSLI
                     AKYQAAGVEIRGCEKTCALVSGIKKATEADWGTEYLDLILSVRVVKDMDEAIDHIERY
                     GSEHTETIVTTNYQRSRDFIDRVNSSAVMVNASTRFNDGGELGLGAEIGISTSKIHAF
                     GPMGLEELTTTKFVVLGDGQIRE"
BASE COUNT          851 a         1099 c          919 g          871 t
ORIGIN      
        1 tccgcagtga tgatcatttt acccttcttg ctcatggccg catccacgac tttgccaata
       61 cattcgtcaa tgacttcgca ggcctgtacc gccgcctcga agttccccgt gtggcccacc
      121 atgtcggcat tagcatagtt agcgatgacc aagccgacgt cctgttcgcg caattgcttg
      181 accagcgcct ccgtcacttt gcgagcgctc atctcgggct ttagatcata agtcgccaca
      241 tccttgggcg aagggatcag aatacgctct tcgccgggaa atttgttctc ctcgccaccg
      301 ttgaaaaagt aggtcacgtg cgcgtacttt tccgtctccg cgatgcggag ctggcggatc
      361 cccgcttcac tcgcgagttc ccctaaaatc tttttgattt ccctaggcgg ataggccacc
      421 ggcactttga aggtttcgtc gtattgcgtc atcgttgcga acgcagaaag agccacgcgt
      481 cgcttgcgcg ggaactcttt gaaatcctct tccgtcagcg cgcgggtcaa ctgccgcgcg
      541 cgatcagctc taaaattgaa gaagatcacc ccgtcggcat cacggatcaa accctcgggc
      601 accacaccgc tgatgatcgt gggaagaaca aactcgtctg taaccttgtc tttgtaactg
      661 ttgcgaatag cctccaacgc cgaatcagct ttattcccga tgccttcggt caagcagagg
      721 tacgccttct cggttctgtc ccaacgtttg tcgcgatcca tggcgtagta tcgcccgctc
      781 accgtggcaa tccgaacctc cggataagcc ttcagcttct cgttgagatc gagcatgaac
      841 tgctcgccgc tgttcggcgg cgtgtcccgc ccgtccaaaa aaagatggag ataaacggtc
      901 ccaacctttt cgcgccgcgc catctcaatc aacgcttcca tgtgacgctg atggctgtgc
      961 acgcccccgt ctcccaaaag acccatcagg tgaagccggc cgctcttatc ccgcgtcttt
     1021 ctcatcgcat ccaaaagatt cttgttccta tagaaactcc cctcgtcgat gtcctggtga
     1081 atgagagtaa ggtcttggta gacgatgcgg ccggcgccga ggatcatgtg gccaacttcg
     1141 gaatttccca tctggccttc aggcaacccg acatccactc cggacataga tagactcgat
     1201 accggaaact cccgagtcaa cctatcgata ttcggaatcg aagcctgagc aattgcatta
     1261 ccctcttttc ttggattgat tccccatcca tccaagatca ccaggatcac gggttgcatt
     1321 atgctcaaga ggctttcctt tcttttgcga ttcccggccg aaagcgcccg caccaagcgt
     1381 aaattaaagc ggcgctcatt ctgctacgat agcggatgat ccgcggcttg aaactgatcg
     1441 accagggttg agtaaggttc gggcagctca actcgaggcg cgtgacccca gagcccgtcg
     1501 agatcgtaga actcgcgcac cggctcgtag aagatgtgaa cgataacgtc ggaaaaatcc
     1561 atcaacaccc aatggccgcg attggcacct tccaccgaaa gcagggagtg accggcctcg
     1621 cttaggttct cttcaattcc ttgcgcaatg ctttgaacct gtcgatcgga gctgcctgaa
     1681 caaataatga aatagtccgc gatggaagtc agttcgcgga cttcgagcac cacgagatca
     1741 cgagcctttt tctccagggc gaaacgcgtt aggagaagga ctttgtccca ggagctaatc
     1801 gctgccaaag ctcttaacgc tcctcccgac gattttgata gaggccctgc tttttgatat
     1861 aagtctcaac tctagaaggc accaagtacc gaatcgattt accctcttct actagccctc
     1921 gtatttccga ggctgaaatg gcgatatccg tgagtctagt aaacaagaca cggctaccgc
     1981 tcccgtgtcg atagtttctc tgcttaaaat cataacaaaa gagctttttt acggcaacgc
     2041 ctgtgccgcg aagcggatcg ctagctttac tgcctggccg ggaagtcaca atgaagttac
     2101 agagtggaaa aatctccgca aaattcttcc atgagccgat ttcctgaaat gcatccaggc
     2161 cgataataaa aaaaagggag tcgcctctgt gctttctttt ggcgaaatgg cgcaccgtat
     2221 cgatggaata ggaaacgccg ggccgtgcaa tctccaccgt cgataccttg aaccggggat
     2281 ttctcgccac cgccaaacga accattgcta agcgatcccg cgccggcgtc gtggtttgcc
     2341 ctcgcttgtg aggcggaatc gaggcgggaa caaaaacgac ccggtcaagc tcaaaagtct
     2401 cactgacctc ctcggcgctg cggagatggc cccaatgaat cggatcgaag gtaccgccaa
     2461 agagaccgat cctcatgggg ttgcaattgg tttcttctgc agaccgctca aaaaggtccc
     2521 agacgcgagg cgcacgaaga tttggcgagc tacaatatca ccgcaaagag tcgaacaatt
     2581 gatcgttgag cctttttcga actcgaaacc cgaaactcga aactcgaaac tgacgaggga
     2641 aagatgaggt tttctgaggg gagtcgtcat tcccggatct gcccatcccc caacacaaca
     2701 aacttggtcg tcgtcagttc ttctaacccc ataggcccaa aggcgtgaat cttgctcgtg
     2761 ctgataccga tctccgcgcc caaccccaac tcaccgccgt cgttaaatcg ggtagaagca
     2821 ttgaccatga ccgccgatga attcactcga tcgatgaaat ctctcgacct ctgataatta
     2881 gtcgtcacta tggtctctgt atgctcggag ccgtagcgct cgatatggtc gatggcttca
     2941 tccatatctt ttacgactcg taccgagaga atcagatcaa ggtattcagt gccccagtcc
     3001 gcctccgtcg ctttttttat tccggaaacc aacgcgcagg ttttttcaca gccccggatc
     3061 tccacgccgg ccgcttgata tttcgctatc agagaaggca aaaaggcggg ggctatcgtt
     3121 tcatgaacga ggagcgtttc catggcgttg cataccgacg ggcgctgcac tttggcgttc
     3181 atacaaatcc gttctgccat ctcatgggac gcgtcggcgt ccacgtagac gtggcatacg
     3241 cctttgtagt gcttgatcac cggcactttc gaatgggtga ccacggcgcg aatcagctct
     3301 tcgccgccgc gtgggataac gagatcgatg tattcttcca gctggagcaa ttcctgaacg
     3361 aaagcatggt ctttggtctg aacaatttgt accgcctccc gcggcacgcg cgtttgcgcg
     3421 caagctttcc ttaagatctg gccgatcgct tggttagaga aatgcgcttc actgccaccc
     3481 cttaggataa ccgcattgcc cgattttagg cacagcgccg cggcgtctgc cgtcacgttg
     3541 ggccgcgcct cataaatgat agcgattacg cctaggggga tccgcatctt gccgacttgc
     3601 agcccgttgg gtcgccgcca cattttcacg acctcgcgca ccggatcagg caaggcagca
     3661 acgtctcgaa ggcctttagc catcgcctga acacgaccgg gattaagcgc aatccggtcc
     3721 actatcggct ctgaaattcc
//
LOCUS       DASSWZ010000060         4532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_808364, whole genome shotgun sequence.
ACCESSION   DASSWZ010000060 DASSWZ010000000
VERSION     DASSWZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808364"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFN58_01415"
     CDS             complement(<1..570)
                     /locus_tag="VFN58_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916675.1"
                     /translation="MAGANGKDYFGKKLSPQESEMVVAELKEYLLGLADRWLFKGRFH
                     TELGKGTLPMEAIQVFWQNWYGFVAEINNFHGVAYQRHLPFFKRHPELQKHFANHVAD
                     EMIHPKPPGHIRIVLEQGRNFGLTDDQMIEYEMLPECRAFLEYYRGVLYEGTMAEWWA
                     SFCVEEAVGHWARFFGGALRKAYKMPEEKI"
     gene            complement(596..2080)
                     /locus_tag="VFN58_01420"
     CDS             complement(596..2080)
                     /locus_tag="VFN58_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8916676.1"
                     /translation="MLSLDQIVGKLEEIVGPRFVSTNPIDKINNQHTLAYGAYRALPD
                     VIVRVESDARGHHSLARILQFANEHKIPIVAKGGVGMGISSPLKGGILLDMLGMDKVL
                     EVNPTLQYVVAEGGASVYAIHHALRQHGLMLPNFGTYGPAVVIGAMVNKGGIGYGMTR
                     YGWIADMVLGLEVILANGETIRLGALANQETTFGPFQKWIHLPDLLGLFTLAAGSMGI
                     ISKVCLRAIEGRREWLHDYCYTFRRDELNRAQDALIQLVRGEVVYDIHFTDRWQYHWP
                     MEEGLYDGSRIPDDAWFFLKTVIMARDAEELAFKENRMRRIYTEAGGVDVPEIADKAM
                     GAKAQQHGLHGWPDYHIMGPDGWCGSVVRHSGMFAVTSKMYPLTFLQEMYDLDEAAKR
                     ASGLWDAEHSPQHDSYVTRDLAIKEEYFVFYNPYDEEDVGKLRKWMGMVQEFSNKRGV
                     VWTAPTLSTKGPYPYERLTNLYDLVKEIKQLVDPNNILNPGALY"
     gene            complement(2132..2905)
                     /locus_tag="VFN58_01425"
     CDS             complement(2132..2905)
                     /locus_tag="VFN58_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8916677.1"
                     /translation="MTEQAKHAPLQGKVALVTGASRGIGAAAAKRLAQSGASVVINYH
                     QNQEAAQRVLEKVEADGGRGMIFQADVTRKDQVEAMVAAVQEKWAAVDVLVNNAYFPF
                     KVGQLHELPWENFHQAVEHELAAFHNCVQACLPGMAAKKAGKIIVISTRLAQQPLPRM
                     GAYAAAKSALESMANTMAIELGPLGIPVNVVTPAFTLTDASMIMPEAFRERVRETRPL
                     KKHLYPEDVAGAIAFLAGDESSMLTGSHILITGGSHLQL"
     gene            complement(2918..4399)
                     /locus_tag="VFN58_01430"
     CDS             complement(2918..4399)
                     /locus_tag="VFN58_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET8916678.1"
                     /translation="MKLLFVTPPMGNWAPWGDRHLAVNSLHSQVAAFIREKEAADVAV
                     LDCRALGLDDNEMMDEVGRRRPDIVFFGSVIPAAGGAAQLNRFHAAMKRIKESYPRTV
                     TVGGGLMYTAIPQQIMKDNPQLDFAIVGVFGDNEYTLWELLGELDRPAPNFDRIKGLT
                     YRRGDEIILTPARPLIQNLDELPMPAYDLFPMDRYYGYSVIPNYNEAVTTRGCEGACH
                     FCYEWWLVDPRNPRDFSSHRTRSGKAVADEMELLNKQYGVKSLTFMDDDFNSDRQKMV
                     DLVEELEKRKLDMSWFCLSRAQNLIRDTDLIPRLRKVGLYQVLVGIDGGTDEEIAEAR
                     KGPMKVGVKELKELIQFLRKNDISTISTYLNGFWEDDEAKIRQRAKAVDEIDPDIVMI
                     QLLNPIPGSPIYKKAVKDSLIEIDNLSLYDLEHCVMPTKHLTRHQLGELTGWAFQSFY
                     GKPGRIDRILNGYSSPYVKMKFLSFKNNAAKYEKGAAEDAVAI"
BASE COUNT         1016 a         1300 c         1158 g         1058 t
ORIGIN      
        1 gattttctct tcgggcattt tgtaggcttt gcgcagagcg ccgccgaaga acctcgccca
       61 gtggccgaca gcctcttcca cgcagaaaga agcccaccat tcagccattg ttccttcata
      121 gagcaccccg cggtaatact cgagaaacgc gcggcactcg ggaagcattt cgtattcgat
      181 catctgatcg tcggttaacc cgaagtttcg tccctgttcg agaacgatgc gaatgtggcc
      241 ggggggcttg ggatgaatca tctcgtccgc cacatgattg gcgaaatgct tctgcaactc
      301 aggatggcgt ttgaaaaaag gcaagtgacg ctgataggcg acgccatgaa agttattgat
      361 ctcagccacg aagccgtacc aattctgcca gaagacctgg atcgcttcca tgggcaaagt
      421 tccttttccc aactccgtat gaaagcgccc cttgaaaagc catcgatccg ccagccccag
      481 cagatattct ttcagctccg cgaccaccat ttccgattct tgcggcgata gtttcttgcc
      541 aaagtaatct tttccgtttg ctcccgccat tgaatttctc cttcctcaac taaaactaat
      601 acaaagctcc aggatttaga atgttattgg gatcgaccag ctgcttgatc tccttcacca
      661 ggtcgtaaag attggtcaga cgttcatacg gatagggacc tttggtgctt aacgtcggcg
      721 cggtccacac aaccccgcgt ttgttgctga actcctgcac catgcccatc cacttgcgca
      781 acttgcccac gtcctcttca tcgtaaggat tgtaaaagac aaaatactct tccttgatcg
      841 ccaggtcacg cgtcacataa ctgtcgtgct ggggtgaatg ctcggcgtcc cagaggccgc
      901 tggctcgctt ggcggcctca tcgagatcgt acatctcttg aagaaaagtc aggggataca
      961 tcttggaggt cacggcgaac atgcctgagt ggcgcacgac cgacccacac caaccgtcgg
     1021 gtcccataat atgatagtcc ggccagccgt ggagcccatg ctgctgcgcc tttgcgccca
     1081 tcgctttgtc ggcgatctcc ggcacgtcta caccgcctgc ctcagtgtag atacgccgca
     1141 ttcggttttc cttgaacgca agctcttcgg catcccgcgc catgatcact gtctttaaaa
     1201 aaaaccaggc gtcgtccgga atccggcttc catcgtacag cccttcttcc atcggccagt
     1261 gatactgcca gcgatcggtg aaatgaatat catagaccac ctcgcccctg accagttgaa
     1321 tcagcgcatc ttgtgcgcgg ttgagctcat cgcggcgaaa tgtgtagcag taatcgtgca
     1381 accattcgcg ccgcccttcg atcgcccgta gacaaacttt actgataatt cccatggaac
     1441 cggccgccaa agtgaataga cccaacagat caggcagatg aatccatttc tgaaagggac
     1501 caaaagtcgt ttcctggttg gcaagagcgc cgagcctgat agtttcgcca ttagcaagaa
     1561 tgacttccag cccgagcacc atgtctgcga tccagccgta gcgcgtcatg ccgtagccga
     1621 taccgccttt gttcaccatg gcacctatca caaccgccgg accgtaggtt ccaaagttgg
     1681 gcagcatcaa gccgtgctgc ctcagggcat gatgaatggc gtagacgctc gcaccgccct
     1741 ctgcgaccac atactgaagg gttgggttga cttccaagac tttgtccatg ccgagcatgt
     1801 cgaggagaat tccacccttg agcggcgaac tgatgcccat acctaccccg cctttggcaa
     1861 cgatggggat cttgtgctcg ttggcaaact ggagaatcct ggcgagggag tgatgccccc
     1921 gggcatccga ttcgacacgt acgatgacgt ccggcaatgc ccgataagcg ccgtaagcca
     1981 gggtgtgctg attgttgatt ttgtcgatgg gattcgtcga aacgaatcgt gggccgacta
     2041 tttcttcgag ctttccaaca atctggtcca gcgatagcat gaagcgaaat cccatcggga
     2101 gaaaatagtc ctgccaagcg gccagagcat atcagagttg aagatgactt ccaccggtaa
     2161 tcagtatgtg gctgcccgtc agcatgctgg attcatcacc cgctaagaat gctatggctc
     2221 cagcaacgtc ctcgggataa agatgttttt tcaacggccg ggtttcacgc acgcgctctc
     2281 taaacgcttc gggcatgatc atggaagcat cggtcagcgt aaaagccggc gttaccacgt
     2341 tgacagggat tcccaaaggc cccagttcga tggccatggt attggccatc gactcgagag
     2401 cggacttagc tgcggcgtaa gcacccatgc gcggcagcgg ctgttgagcc agccgggtgc
     2461 tgatgacgat gattttgccg gctttctttg ccgccattcc cggcaaacag gcctgaacgc
     2521 agttgtggaa ggcggcaagc tcatgttcga cggcctgatg aaaattctcc catggcaatt
     2581 catggagttg acccactttg aaagggaagt aagcgttgtt gaccagcaca tcgacggcgg
     2641 cccacttttc ttgaactgca gccaccatcg cctccacctg atccttccga gttacgtccg
     2701 cttgaaagat catgcctcga ccgccatcag cctcgacttt ctcgagaacc ctttgcgcgg
     2761 cttcctggtt ctggtggtag ttaatgacga cggacgcgcc ggactgtgca agacgctttg
     2821 ccgcggccgc accgataccg cggctcgccc cggtgacgag agcgactttt ccttggagcg
     2881 gtgcgtgttt agcttgctcc gtcatgaact caaaagctca aatggcgacg gcgtcttccg
     2941 ccgcgccctt ttcatacttc gcggcgttgt tcttgaagga gagaaacttc atcttcacgt
     3001 acggcgagct gtaaccgttc aaaatccggt cgattcgccc cggtttgccg taaaaggatt
     3061 gaaaagccca accggtcaat tcgccgagct gatggcgcgt gagatgtttg gtcggcataa
     3121 cgcaatgctc gaggtcgtac agactgaggt tgtcaatttc gataaggctg tccttgaccg
     3181 cctttttgta gatgggcgat cccgggatcg gattgagcaa ctggatcatg acaatatccg
     3241 gatcgatctc gtcaaccgcc ttggcccgct ggcgaatctt cgcttcatcg tcttcccaga
     3301 aaccgtttaa ataggtcgag atcgtcgaaa tgtcattctt acgcaggaac tggatcagct
     3361 cctttaactc cttgacaccg actttcatcg gccctttgcg cgcctcggcg atctcttcat
     3421 ccgtcccacc atcgatgccg actagcacct gatagagacc aaccttacgt aaccgcggaa
     3481 tcaagtctgt gtcgcggatg agattctgtg cccggctcag gcagaaccag ctcatgtcca
     3541 gttttctttt ttccagctcc tccaccaagt cgaccatctt ctggcggtcg gagttgaagt
     3601 catcgtccat aaaggtgagc gacttgacgc cgtattgctt gttcaataac tccatctcgt
     3661 cggcgaccgc cttaccgctg cgggtacggt gcgagctgaa gtcacgtgga ttgcgcgggt
     3721 caacgagcca ccactcgtaa caaaagtggc aggccccttc gcaacccctg gtggttaccg
     3781 cttcgttgta gttcggaatg acggaatagc cgtagtagcg atccatcggg aaaagatcgt
     3841 aggccggcat gggaagctca tcgagattct ggatcaaggg cctagccggg gtgagtatga
     3901 tttcgtcgcc cctgcgatag gtaagccctt tgatcctgtc aaagttcggc gccgggcgat
     3961 cgagctcccc caatagttcc cacaacgtgt attcattatc gccgaagaca cccacgatcg
     4021 cgaaatcaag ctgcggatta tctttcatga tctgttgggg gatggcggtg tacatcaaac
     4081 cgccaccgac ggtgaccgtc ctaggataag attccttgat ccgtttcata gccgcgtgaa
     4141 accggttgag ttgtgccgcg ccgcctgcgg ctggaatcac gctaccaaag aatacaatat
     4201 ccggtcgtcg ccggccgacc tcgtccatca tttcgttatc atccaagccg agggcgcgac
     4261 aatctagaac cgcaacgtcc gcagcttcct tttcgcggat aaaagcagca acttgcgagt
     4321 gcagggaatt taccgcgaga tggcgatcac cccagggtgc ccagtttccc atcggcggcg
     4381 tgacaaatag gagcttcatt aggttttcct cgcttacgta gaatcgtttg actcaaaata
     4441 tttaatacct aaacgattgt caagttagac gtacaaaatc gggcccttcg gagtttcaac
     4501 cccagcgccc agaaatgaaa ataaaaggcc at
//
LOCUS       DASSWZ010000061         3809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_327343, whole genome shotgun sequence.
ACCESSION   DASSWZ010000061 DASSWZ010000000
VERSION     DASSWZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327343"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..574)
                     /locus_tag="VFN58_01435"
     CDS             complement(17..574)
                     /locus_tag="VFN58_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET8916679.1"
                     /translation="MAQDRKKLWMSPFTFMILLSLLGCKGSQKEAADSKELPDLNAQI
                     RPYITIDNTKLRTGPGPQFRAIAEIRRDAKVQVVGRDGDWVLIVSKKGNPPGYIEMAS
                     IEPSSGVEKQESSAPPVEGKYESLVDTRVYSGPGLHYPVVAEIKKGTKINVVDEEKGW
                     LKVESKRGNKPGYVEASIAKPVGSQ"
     gene            complement(686..1105)
                     /locus_tag="VFN58_01440"
     CDS             complement(686..1105)
                     /locus_tag="VFN58_01440"
                     /inference="COORDINATES: protein motif:HMM:NF020489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HET8916680.1"
                     /translation="MKLLCFQAKHFRWKTYSKTLVEVADQDVDEQVADAIVIFVHAEK
                     SDEEADKKPSVLRQTLKHIKWLANKRDFKNIVIHSFTHLGGETAAPDFAEALIKAVAE
                     RLRENTYTVWTTPFGYFCEWDLSVYGDSLAKVWKDIH"
     gene            complement(1113..1679)
                     /locus_tag="VFN58_01445"
     CDS             complement(1113..1679)
                     /locus_tag="VFN58_01445"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET8916681.1"
                     /translation="MILLIDNYDSFTYNLYHYLGELGTKVEVFRNNRISLDDIELMRP
                     EKIVISPGPCTPKEAGISCDVILRFAEKTPILGVCLGHQCIGAAFGGEIIRAPSIMHG
                     KLSEVSHDSQTIFRSIKSPFAAMRYHSLVINPEHLPAELSISARTADNVIMGVRHKRF
                     PVEGVQFHPESILTDEGKRLLKNFLDYY"
     gene            complement(1676..3163)
                     /gene="trpE"
                     /locus_tag="VFN58_01450"
     CDS             complement(1676..3163)
                     /gene="trpE"
                     /locus_tag="VFN58_01450"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET8916682.1"
                     /translation="MIRPSFKEFCRFAEQGNLIPVYQELLMDLETPLSFFKRLERDDY
                     SFLLESVEGSERWARYSFLGTRPQRIFKARGTQVEIIENGKKISFTSDSPLKSLEDLL
                     KGCRPVTVPGVPPFFGGALGYVAYNAVEQFHEISDGKKDPLAMPEIFFIFVQSVVAFD
                     NLKHTIKVIDNVRVEQRKNLRRAYDQASQRIRKVISSLQKKPRGIEPRDLSQEQGNRK
                     FRSNLTREEFRNAVIRAKDYIKAGDIIQVVLCQRLETETASDPFEIYRALRFINPSPY
                     MFYLELEDLRVIGSSPETMVRLTGDTIELRPIAGTRRRGATPEEERELEADLLADPKE
                     CAEHIMLVDLGRNDVGRVAEIGSVEVNELMAIERYSHVIHIVSNVRGKLAAAKTPFDL
                     FVSAFPAGTVSGAPKIRAMQIISELEPEKRGLYAGAIGYFAYNGNLDTCIVIRTIVMK
                     GKKVFINAGAGIVADSDPEMEYQETLNKARAMLKAVDLAEQWRKK"
     gene            3645..>3809
                     /locus_tag="VFN58_01455"
     CDS             3645..>3809
                     /locus_tag="VFN58_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8916683.1"
                     /translation="MVRREKSNYTIQSVSHALDVMEQFNGSVDEIGVTELSKRLKLHK
                     NNVFRLLATLE"
BASE COUNT          860 a          953 c         1006 g          990 t
ORIGIN      
        1 actgaccgcg ctacgctcac tggcttccga cgggcttcgc gatgctcgct tcgacatagc
       61 ccggtttgtt gccgcgcttg gattcgacct ttaaccagcc tttttcttcg tccacgacgt
      121 tgatttttgt tccttttttg atttctgcca ccaccgggta gtgaagccct ggtccgctat
      181 acactcgggt atcgactagg ctttcatatt taccttctac aggcggcgcg gatgattctt
      241 gtttctcaac gccactactg ggttcgatcg atgccatttc gatgtagcca ggtgggttgc
      301 ctttcttcga aacaatcagc acccaatcgc catcccgtcc aacgacttgg accttggcat
      361 cgcgcctgat ttctgcgatg gctcgaaact gtggtccggg cccggtgcgt agcttggtat
      421 tgtcaatagt tatgtaggga cgaatttggg cgttgaggtc cggaagctcc ttcgaatcgg
      481 cggcttcttt ttggcttccc ttgcaaccca ataaactcaa taaaatcata aaggtaaagg
      541 gggacatcca cagttttttt cgatcttgag ccatgttctc tccttattaa tgaaaagaga
      601 actccattat aacacgctcc gcgatttgga tgtttttgtt agcgccggca ggtcgcgtgc
      661 ttggcgatgg ttgtagatta gggaatcagt gtatgtcttt ccaaaccttg gccaagctgt
      721 cgccgtagac gctgaggtcc cactcgcaga agtaaccgaa aggtgttgtc cagactgtat
      781 aagtgttttc gcggagacgt tccgcgacag ctttgatgag agcttcggcg aaatcagggg
      841 cggcagtctc gccgcccagg tgggtaaagg agtggatgac gatattcttg aagtctcttt
      901 tgttggcaag ccacttgatg tgctttaacg tttgccgcag gactgaaggt tttttgtctg
      961 cttcttcgtc ggacttctcg gcgtggacga agataacaat cgcgtccgcg acctgctcat
     1021 ctacgtcctg atccgctacc tcgactaaag tcttggagta ggtcttccaa cgaaaatgct
     1081 tcgcctggaa acaaagaagt ttcatgtaga gatcagtagt agtccaggaa attcttaagt
     1141 agtcttttgc cctcgtcggt aagaatcgat tcgggatgga attgcacgcc ttccacgggg
     1201 aagcgcttgt gacgcactcc catgatgacg ttgtccgcgg tacgggcgct gatgctcagc
     1261 tcagccggca gatgctcggg attgatgacg agggagtgat agcgcatggc agcgaaggga
     1321 cttttgattg accggaagat tgtctgtgaa tcgtgactca cctccgagag tttgccatgc
     1381 atgatcgacg gcgcccggat gatctctccg ccaaaagcag cgccgataca ctggtggccg
     1441 agacagacgc ctagaatggg tgtcttctca gcgaagcgca aaatcacgtc acatgagatt
     1501 ccagcctctt taggcgtaca gggaccgggc gagatgacaa ttttctccgg tctcatgagt
     1561 tcgatatcgt caagactgat ccggttattg cgaaagactt cgaccttcgt ccccagctcg
     1621 ccgagataat gataaaggtt gtaagtgaag gagtcgtagt tgtcgataag cagaatcact
     1681 tctttctcca ctgctcagcc aggtcaacgg ccttcagcat ggcccgggcc ttgtttaagg
     1741 tctcctgata ctccatttcg ggatcggagt cggcgacaat ccccgcaccg gcattgataa
     1801 agactttttt tcctttcatc acgatggttc gaatgacgat gcaggtgtcg agattgccgt
     1861 tgtaagcgaa gtagccaatg gcgcccgcgt acagaccccg cttctctggt tccaattcgc
     1921 tgataatttg catggcgcga atcttcggcg cgccggagac ggtgccggca ggaaatgcgg
     1981 acacaaagag atcaaacggt gtctttgcgg ccgccagttt tccgcggacg ttggagacga
     2041 tgtgaatgac atgggaatag cgctcgatgg ccatcagctc gttgacttcc accgagccta
     2101 tttcggcaac acggccgacg tcattgcggc ccaagtccac gagcatgata tgctcggcgc
     2161 actctttagg atccgccaac agatcggcct caagctcccg ctcttcctcc ggcgtggcgc
     2221 ccctacggcg ggtgccggcg atgggacgga gctcgatggt gtcgccggtc aggcggacca
     2281 tggtttccgg tgaagagccg atgacgcgaa ggtcttcgag ttcaagatag aacatgtagg
     2341 gtgacggatt gataaaccga agcgcacgat aaatctcgaa agggtcgctc gccgtctcgg
     2401 tctcgagtcg ctgacagagc acgacctgaa taatatctcc cgccttaata tagtctttgg
     2461 cgcgaatgac cgcattgcga aactcttctc gcgtcaggtt ggagcgaaat tttctattcc
     2521 cttgctcttg actcaagtct cgtggctcga tcccgcgggg tttcttttgc agagacgaga
     2581 tcacttttcg gattctttgg cttgcctgat catacgcccg tcgaaggttc tttcgttgtt
     2641 ccactcgtac gttatcgatc actttgatgg tgtgttttag attatcgaag gcaaccacgc
     2701 tttgaacaaa aataaaaaag atttcgggca tagccaaagg atcttttttg ccgtcggaaa
     2761 tttcatggaa ttgttcaaca gcgttatagg ccacgtaacc gagagcgccg ccgaagaagg
     2821 gaggaacgcc gggtaccgtg acaggccggc agcccttgag gaggtcctct agcgatttca
     2881 gcggtgagtc tgacgtgaag cttatctttt ttccgttttc aatgatctcg acttgagttc
     2941 cccgggcttt gaagatccgt tgagggcgtg ttcccagaaa agaataacgg gcccagcgtt
     3001 cgctcccttc tacgctttca agtaaaaagg aatagtcgtc gcgttcgagc cgcttgaaga
     3061 aggaaagcgg agtctccaga tccataagca attcctgata tacaggaatc aggttgccct
     3121 gctcggcgaa ccggcaaaac tctttaaagc tcggtcgaat catttttttt acaaacaaaa
     3181 agggccataa gttttcacct atggcccttt cccttaaccc aacgaataaa ttgactggaa
     3241 ttagccacgg tgcggacgct gccgcagttc ccagcgccac caaccgaaag ctaactttcc
     3301 gttctccagc cgattgttca cattcggcat tttgccttat ctgttcaggg gggtcaaggc
     3361 ttgtttcaaa acagaagtct gaaagtcgca agggtagttg tgttggccta agctatttat
     3421 gaatatttag aaggattagg actttgagga aagtcgactc ctattgatag gcgccggatg
     3481 tttacctcgg aatggcaaag tcggaatcga ttttgcggag gtaaaggcgc cgcaaccact
     3541 atttgacaac cgtcgcgcgc taagttaaaa cgcctttttg caccgatcct tgttggttga
     3601 tgaccgggca gatttcaacg gaactagttc atccaggagg agacatggtc agacgcgaga
     3661 aatccaatta tacgattcag tctgtttctc atgctctcga tgttatggag cagttcaacg
     3721 ggagcgtcga tgagatcggt gttactgaac tcagcaagcg gttgaaacta cataagaaca
     3781 atgtcttccg cttgctcgcg acgctcgaa
//
LOCUS       DASSWZ010000062         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1332055, whole genome shotgun sequence.
ACCESSION   DASSWZ010000062 DASSWZ010000000
VERSION     DASSWZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332055"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /gene="ligA"
                     /locus_tag="VFN58_01460"
     CDS             <1..1275
                     /gene="ligA"
                     /locus_tag="VFN58_01460"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET8916684.1"
                     /translation="FLDAIKEWGLKPVPFTRICRSLKEILAYQEEMEAQRDKLPYEID
                     GLVIKVNSTELQRRLGEIARSPRWAIAYKFKARQAATRILDIQPQVGRTGTLTPVASL
                     EPVPIGGVTVKSASLHNMDEIERKDIRTGDTVVVERAGDVIPYVVQVLTDKRTGEERR
                     FIMPDHCPVCGSGVYREEGEAAYRCIGISCPAKLKESLKFFGSRGAMDIEGLGDKLIE
                     QLVERGLVKDLADLYKLNKTELANLERMAEKSAQNLLNALERSKEATLPRCLTALGIR
                     HVGEATAKLLAEHFGDLASIMEASEEQLTEVREIGPQVAKSIAQFFAQKENREVIKKL
                     LEAGVRFRREPRREGALAGSTFVLTGGLETMSRVEAQRRIEALGGRLASNVSRNTSYV
                     VVGADPGSKLKKAKELGLRILDEKELKKILEG"
     gene            complement(1277..1585)
                     /locus_tag="VFN58_01465"
     CDS             complement(1277..1585)
                     /locus_tag="VFN58_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET8916685.1"
                     /translation="MGAKLYVGNLPYSVTEERLQQHFAQHGSVVSARIITDKFSGRSK
                     GFGFVEMSSDQEAERATAALNGTDFEGRNIVVSEARPQQQRAPRPMGGGGRPPRGNRY
                     "
     gene            1840..>2129
                     /locus_tag="VFN58_01470"
     CDS             1840..>2129
                     /locus_tag="VFN58_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET8916686.1"
                     /translation="MKEAARVHLKVHGRVQGVYFRASTVEEARRLRLTGWVMNCPDSS
                     VEVVAEGEREQLEKLVSWCRSGPPGARVREVRAEWEAPKEEFQSFFIRRNFD"
BASE COUNT          506 a          559 c          636 g          428 t
ORIGIN      
        1 tttctcgatg cgattaagga gtggggcttg aaacccgtgc cgtttactag aatctgccgc
       61 agcctcaagg agatccttgc atatcaagaa gaaatggaag cgcagcgcga caagcttccc
      121 tatgaaattg acggcctcgt gatcaaggtc aacagcaccg aattacaaag acgcttggga
      181 gaaatcgcgc gctcaccgcg ctgggccata gcctacaagt tcaaggcgcg ccaggcggcg
      241 acgcggattc tggatattca accccaagtg ggccgcaccg gcaccttgac cccggttgcg
      301 agcctggagc cggtgcccat cggtggtgta acagtcaaga gcgcgtcgct gcacaacatg
      361 gatgaaatag aacgcaaaga catccgcact ggagacaccg tcgtggtcga gcgcgccggc
      421 gacgttattc cctacgtcgt acaagttttg acggacaaaa gaacaggcga ggagcggcgc
      481 tttatcatgc cggaccattg tccggtatgc gggtccgggg tctaccggga agaaggtgag
      541 gcggcctatc gttgcatcgg catctcgtgt cctgcgaagc tcaaagagag cctcaaattc
      601 ttcggctcgc gcggcgctat ggacatcgag gggctcggcg acaaactcat cgaacaattg
      661 gtggaacggg gtttggtgaa agatttggcc gatctttata aactaaataa aacagagctg
      721 gccaatctcg agcgtatggc cgagaaatcc gcgcagaatc tcctcaacgc cttggaacgc
      781 agcaaggagg cgactctacc acgctgtttg actgccctcg gaattcgtca tgtaggtgaa
      841 gccacagcca agcttctcgc cgagcacttc ggcgacctgg cgtcgattat ggaagcgtcc
      901 gaagagcaat tgacagaggt aagggagatc ggtccccaag tggccaaaag tatcgctcag
      961 tttttcgccc aaaaagaaaa ccgcgaagtc atcaagaaac tgctcgaagc tggagtccgc
     1021 ttcaggcgcg aacccaggag agaaggtgcc ctcgcgggat cgaccttcgt tctaacgggt
     1081 ggactggaga ccatgtcacg agtggaagcg cagcggcgga tcgaagcgct tggcgggcgc
     1141 ctcgcttcca acgtgagccg gaacaccagc tacgttgtcg tcggcgccga tcccggctcc
     1201 aaactcaaga aggcaaagga actcgggctt cggatcttgg atgaaaaaga gctaaagaaa
     1261 atattggagg gctgacttaa tagcgattgc cgcgcggcgg cctgcctcct ccgcccatcg
     1321 gtcgcggcgc tcgctgctgc tggggtcgcg cttcgctgac aacgatgttt ctcccctcga
     1381 aatccgtgcc gttgagggcg gctgtggctc gttcggcctc ttgatcggaa ctcatttcca
     1441 cgaaaccgaa ccccttggat cttccgctga atttatcagt gatgatccgg gcgctcacca
     1501 cggagccgtg ctgagcgaag tgttgctgga gccgctcttc ggtcacggag taaggaaggt
     1561 tgcccacgta tagctttgct cccatattgg ccctcctagt tgcacctaga aactatactt
     1621 ttcgggatcg cttacccaaa gggcagggcc tcaaacggtc tcaccagcgg gagccaatct
     1681 ctcggcgcgc tcttcggtaa aatgaccctt tcagaccggc gtaagcctac ctaaggagtg
     1741 ggtctgttgt caaatctatt cggcattgga ctttcgtatg taaaagttgc gacgattgcg
     1801 taggtaagat atgagaatgg ccgagcgaaa ataacgacga tgaaagaagc ggcgcgtgtt
     1861 catttgaagg tccatggcag ggtccaggga gtttacttcc gagcatcaac ggtggaggaa
     1921 gcccgacggc tgcgtctgac ggggtgggtt atgaactgcc ctgatagctc ggtagaagtg
     1981 gttgcagaag gcgaaaggga gcaattggaa aagcttgtca gctggtgccg cagcggacca
     2041 ccaggcgcgc gagtgcggga agttcgcgcc gaatgggaag cgccaaagga agaatttcag
     2101 agtttcttca taaggcgcaa tttcgacta
//
LOCUS       DASSWZ010000063         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1190600, whole genome shotgun sequence.
ACCESSION   DASSWZ010000063 DASSWZ010000000
VERSION     DASSWZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190600"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFN58_01475"
     CDS             complement(<1..813)
                     /locus_tag="VFN58_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET8916687.1"
                     /translation="MKAIQIQSHGGLEVLRVTTLPEPQLVADEVLVRIKASAVNPLDG
                     IIRMGDFPIAKKPPLILGEEAAGIVERNGAGFKAGERVIIYGGGLGVFRDGTWAEAVA
                     VPASSLRRLPDAISFAEGAALTNVGVTAYGALRVAELKAGETLVVLGATGGVGSAGVQ
                     IGKAIGARVIAVVSTVGKAARLQALGADHVIALSDGPLAEQVQELTESRGADVVLDTV
                     GGDLTGRALSSLASFGRLVHLGYGAGTTLTIDSLDLIAKPSTIIGFNIFLVPA"
     gene            956..1270
                     /locus_tag="VFN58_01480"
     CDS             956..1270
                     /locus_tag="VFN58_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916688.1"
                     /translation="MKVSSCAVALYLKLDKTNTCHCRPKKDAALVGLSEFFKLKLDKA
                     GKTADVRVQKRWIFLTGIGSLGKVGNNGIQQFSFCNLTTEWSHFATTICRYVFESVKS
                     TG"
     gene            1344..2336
                     /locus_tag="VFN58_01485"
     CDS             1344..2336
                     /locus_tag="VFN58_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8916689.1"
                     /translation="MIWLILIVGLIIIGNLGIRGYYRKWLKVQTKRVSADSKIIQTSR
                     GQVEYDMRGHGPTVLHFHGGNVGHNGWFFLAHLVEGGYRLLTPDRPGYLGTLLANNGS
                     PEAQADLAAVLLDALAIDTVAVVGISAGGPGALQFVLRHPDRAKALILLSAISRRTGL
                     SEDQMNSTLGRLVMTPRFQNPAYFLINQAMKRIPSLALQDYVRTETTYDMKTGKRYIK
                     QILADPAQRQQVMDLADAIVPALPRFEGVSNDLNVQQSLDNLPLEQIQIPTLIVGSKN
                     DGDIGYENSTNSHAKIPNAELITVEQFGHFIWWGDAQVTKDFQKRIEIFLEQGG"
     gene            2333..3388
                     /locus_tag="VFN58_01490"
     CDS             2333..3388
                     /locus_tag="VFN58_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET8916690.1"
                     /translation="MRRSGKTLLVLFASMLILCLATFGLEFKLAHDRRKAEAKWSKQV
                     RPYYWPSLQTRDGIALGTRSGPLKLVLHPFVSYANLPNQKTPHFSINNLGFRGGDIRK
                     DEKTKKRIIVVGGSTAFGTGLQHDDETFAQRLEYLLDAEVINAAVVGHASGQELVSLV
                     MELVDLQPDLVIALNGWNDFERPLTSKFRGSNGFADIEEQLKTLQSVTDESLLTRVSN
                     LYWVLFPNITKQLESLWLRFFRGGQTRETDLGLASAVYATNMTKMRRISEAFHSTFLC
                     VLQADRDSHQRYRAFRETAKMHLQQVGIVPLDLSELDEPGIKAEWYMDEMHLDAAGNQ
                     AMAQIIANKIVADGLLR"
     gene            complement(3588..>3724)
                     /locus_tag="VFN58_01495"
     CDS             complement(3588..>3724)
                     /locus_tag="VFN58_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET8916691.1"
                     /translation="LRIAVNLRLAEPEAVAQIPIDHFDGLNTFDDLPRDGRCVSDYWF
                     "
BASE COUNT          927 a         1002 c          954 g          841 t
ORIGIN      
        1 cgctggcacc agaaagatgt tgaacccgat aatcgtggac ggcttcgcga taagatcaag
       61 ggaatcaatt gtcagggtcg ttcccgcacc gtagccgagg tgaaccagcc gcccgaacga
      121 tgcaaggctg gatagtgcac ggcctgtcag atcgcctccg acagtatcga gaaccacatc
      181 tgcccctctt gattctgtta gctcttgcac ttgctcggcc agcgggccgt cagacagagc
      241 tatcacatga tctgctccaa gggcctggag cctcgctgcc ttccccacag tggacacaac
      301 tgcgatgacg cgagccccga ttgccttccc aatctggacg ccagccgagc caaccccacc
      361 ggtcgctccg agaacgacca gcgtctcgcc agctttcagt tccgccaccc gcagtgcgcc
      421 gtaagcagtc acgccgacat tggtcagcgc cgcgccctct gcgaatgaaa tggcgtccgg
      481 caatcgccgc agcgaagaag ccggtaccgc taccgcctca gcccaggtcc catcacgaaa
      541 tactcccaag cctccaccgt agatgatgac tcgctcgccc gctttgaacc cggccccgtt
      601 ccgttcaacg atgccagctg cctcctcgcc tagaatcagc gggggcttct tggcgatagg
      661 aaaatcaccc atccgaatga tcccatcaag cgggttgact gccgatgcct taatgcgtac
      721 gagaacctca tcagcgacca actgcggttc aggcaacgtc gtaaccctga gaacttccaa
      781 accaccatgt gactgaatct gtattgcttt catgatcaat cctatcactt atgcaattgt
      841 catctagctt cagttccttt agggtttgag ttaactgaca gcatccctca tttcgattcg
      901 ccgcgcaaag gtcggtaacc gccaaatata tccacaagcc accgctgcga agaacatgaa
      961 agtatcttct tgcgcggtag cgctgtacct gaagttggac aaaacaaaca cctgccattg
     1021 tcggccaaaa aaagatgcgg ctctcgtagg tctatctgaa tttttcaagt taaaacttga
     1081 taaagccgga aaaactgccg acgttcgagt tcaaaaacgc tggatatttc ttacaggaat
     1141 aggaagcctt ggaaaggttg ggaataatgg cattcaacag ttctccttct gcaatttaac
     1201 aacggaatgg tctcactttg ccacaaccat ttgtcgctat gtgttcgaat cggtaaaatc
     1261 gaccggctga gacagccgtt aaacgcttga atagatacca atagcgactg atagatccga
     1321 cgattgagaa tgagaaccgg atcatgattt ggctcatttt gatagttggt ttgatcatca
     1381 tcggaaacct cggcattcgc gggtattacc gtaaatggct caaagtacag acgaagcgcg
     1441 tttccgcaga tagcaaaatt atccagacat cccgtggaca ggttgaatat gatatgcgtg
     1501 ggcatggacc tacagtcctt cattttcacg gcgggaatgt ggggcacaac ggttggttct
     1561 ttctggcgca tctcgtcgag gggggatatc ggctgctgac accggatcgc cccggatact
     1621 tggggacgct gttagcgaac aacggatcgc cggaggcaca ggctgatctc gctgcagtgt
     1681 tgctcgatgc gttggcaatt gataccgttg ccgttgttgg catctcggct gggggaccag
     1741 gtgctctgca atttgtgcta cgtcacccgg atcgagcaaa agctctcatt ctcctttcag
     1801 ccatttctcg ccgcaccggc ttatcggaag atcaaatgaa cagcacgtta ggacgtttgg
     1861 tgatgacacc ccgtttccag aatcccgctt atttcctcat caatcaggcg atgaagcgaa
     1921 taccttcatt ggctttgcaa gactatgttc gcacagaaac cacttatgat atgaaaactg
     1981 gtaagcgtta catcaaacaa attctcgccg atccagccca acgccagcag gtgatggact
     2041 tggctgatgc cattgtgccc gcgttgcctc gctttgaggg cgtatccaac gatctcaatg
     2101 tccagcaaag tctggataat ttgcctctcg agcagattca aattccaacg ctcatcgttg
     2161 gcagcaaaaa tgatggtgac atagggtacg aaaattccac aaactcccac gccaaaatcc
     2221 ctaacgcgga actcatcacc gtggagcagt ttggacactt tatctggtgg ggcgatgcgc
     2281 aggtgacaaa ggattttcaa aaacgaattg aaatattctt ggagcaaggc ggatgagaag
     2341 atcggggaaa acactacttg ttctgtttgc aagcatgttg atcctttgtc tcgccacgtt
     2401 cgggttggag ttcaaactgg cccacgaccg gcgtaaagca gaggcaaagt ggtccaagca
     2461 ggtgaggccg tattattggc cgtcgctcca gacgcgcgac ggcatcgctc tcgggacgag
     2521 atccgggccg ttaaaattag tcctacatcc gtttgtcagt tacgccaact taccgaacca
     2581 aaaaaccccg cacttttcga tcaataacct cgggtttaga ggcggcgata tccggaagga
     2641 cgaaaagact aaaaagcgca ttattgtggt cggaggctcc acggcatttg gcaccggtct
     2701 tcagcatgac gacgaaacct ttgcacaacg cctcgaatat ctgttggatg ccgaagtgat
     2761 caacgctgcg gtcgtcggtc atgcctccgg acaggaactg gtgtctctgg taatggaact
     2821 cgtggactta cagccggatc tggtgatcgc tctgaatggg tggaatgact ttgagcgtcc
     2881 tctgaccagc aagttccgcg ggtcaaatgg ctttgcggac atcgaggagc agctgaaaac
     2941 gttacaatct gtgaccgatg agtcgttgct cacgagggta tcgaacctgt attgggttct
     3001 gtttcccaac atcaccaagc agctggaatc actgtggctc aggttcttca gggggggaca
     3061 aacacgtgaa acggatcttg ggctggccag tgcggtctac gcgaccaata tgaccaaaat
     3121 gcgcaggatt tcagaagcct ttcactccac atttctctgt gtgttgcagg ctgatcgaga
     3181 tagccaccag cggtaccggg ccttccgcga aacagcgaaa atgcatttac agcaggtagg
     3241 gattgtcccg ctggatttaa gcgagctcga tgagccgggc atcaaagctg agtggtatat
     3301 ggatgaaatg catttggatg cagccggcaa tcaggcaatg gcccagatca tcgcaaacaa
     3361 gatcgtcgct gacggcctac tccgctaagg tgcatatgct gcggaagctt gcatattcca
     3421 ccggatctca aggctgaacc caagtcggaa cctcctcact ttgccagaac tatatgccac
     3481 tatgtgctcg aatcggtaaa atcgatcggc tgagaccgcc gttaaacgct tgaatagata
     3541 ccaatagtga ccgatggtga cacatagtcc cgctacggcg ccaagcccta gaaccagtag
     3601 tcagaaacac agcgaccatc tcgcggtagg tcgtcaaatg tgttcaggcc gtcgaagtgg
     3661 tcgatcggaa tctgcgccac agcttcgggc tccgccagac gcaggttcac ggcgatccgg
     3721 agac
//
LOCUS       DASSWZ010000064         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1388563, whole genome shotgun sequence.
ACCESSION   DASSWZ010000064 DASSWZ010000000
VERSION     DASSWZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388563"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="VFN58_01500"
     CDS             complement(<1..2252)
                     /locus_tag="VFN58_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8916692.1"
                     /translation="MSRFFVYHPIIAIVIAILMVIVGIVAIARLPIEQYPNLAPPEIL
                     LQTNYVGADALTVEQSVATPIEQQMQGVDNMLYMISVNDNSGQMRLRVDFDVDTKPND
                     DQILTHLRYLQAESYLPQDVRNYGVTNRKSPTSPLALFSLYSPKGTYDATFLANYAYI
                     NIYDPMSRVPGIGQVLIFGAGQYAMRFWVRPDTLASMGITVTDIITALTNQNKVNPAG
                     QIGGEPVPRGQQFTYTVRAQGRLLTAEEFGEVVVRANPDGSVVRMKDVARVELGAQSY
                     SQIGRLNGNPSAIIAVYQLPDTNALDTMKSAKALMEELKTRFPEDLDYIVSLDTTLAV
                     SQGIKEIVTTLVEALILVVLVVFIFLQNWRSTLIPLLAVPVSLVGTFAVFPFLGFSIN
                     TLSLFGLVLAIGLVVDDAIVVVEAVEQHIEKGLSPRDATLKAMEEVAGPIVAIALVLA
                     AVFLPPAFVGGISGRLNNQFAVTIAVSTLFSAFNALTLSPALSALLLKPGKESSGLLG
                     RFFNSFNSGFGKVMHFYLVGSDVLLHKVAVSLLLLLVIAVSAGFVGSRLPTGFIPEED
                     QGYFYMNVQLPTASSLQRTGDVAKKIEAILSETPGVQTYNTVVGFSLLSLANISYNAF
                     FFVTLKPWDERGSGLTAAAIIQKLNQRLAGLPDAQAFAFSPPAIPGIGTAGGVTFMLE
                     DRSGQAIDFLAQNTDRFLQAARQRPEFAALTTTFIPNAPQVYADVDRDKVLNQGVDLS
                     LVYQTLQTFMG"
     gene            complement(2258..3460)
                     /locus_tag="VFN58_01505"
     CDS             complement(2258..3460)
                     /locus_tag="VFN58_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8916693.1"
                     /translation="MCLPFEKLRLFVAAFLIFNPILSLIGCQKAQQPASTTPDVEVVE
                     VLQKDVPIWKEWIGTLDGLWNAQIRPQVTGYLLRQTYKDGAFVKKDQLLFEIDPRTFQ
                     AAVDQYKGQLANAEGLLASAQANQVKTQNDVNRYTPLAKEQAIPQQDLDTAIQVNLGA
                     KAQVDAAKAQIEAAKGQLASAQLNLGFTRVLSLIDGIAGIAQGQLGDLVTPSTLLTTV
                     STVDPIKIYFPVSEREYLDYVKEHPDEDKRHLRLQLILANGLVYPHEGQTTLVDRQVD
                     VKTGTLRVQGLFPNPGNVLRPGQYARVRTIIATRRGALLVPQKAVSELQGNYQVAVVG
                     SDNKVQIRPVKVGEQVGTEWIIEQGLKPGERVVAEGVQKVRGGLTVNPKPLTTTAEAQ
                     PAPAAPTR"
     gene            3655..4506
                     /locus_tag="VFN58_01510"
     CDS             3655..4506
                     /locus_tag="VFN58_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8916694.1"
                     /translation="MRRFELVEPRTLEEACGLLSDNNEARAIAGGTALLTLIKHGVFL
                     PKTLVNLKKIKGATEIDYDSQRGLRIGALATIYDVESSPLVQRHYPVLGAACHVVANI
                     RIRNVATIGGNLAHGDYQSDPPTVLVALDAGLELMRRNATRQIKIADFLRGSYETALE
                     PGELVAAILIPPAADLKGAYIKFTTGSSEERPCAGIAVLAKMENSTCRSLAIVVGAVS
                     PRPVRIDKAEEMASGQRLDVKLMEQIATEASRAVDPIDDLRGSADYKRHLVQVLVPRA
                     LSTVAQT"
     gene            4522..>4791
                     /locus_tag="VFN58_01515"
     CDS             4522..>4791
                     /locus_tag="VFN58_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8916695.1"
                     /translation="MAVVGTNVTMVGGLAKVTGAVNYAPDLVLPRMLYAKALRSPYAH
                     ADLLRVDASKAEKLPGVVAVITRDDLAGLSPYFGPVVDDQPVVATD"
BASE COUNT         1075 a         1319 c         1369 g         1028 t
ORIGIN      
        1 cccatgaacg tttgcagcgt ctgataaacc aaactgaggt ctacaccctg gttcaacacc
       61 ttgtcgcgat ctacatccgc ataaacctgc ggcgcgttcg ggatgaatgt cgtagtcagc
      121 gcggcaaact cgggccgctg ccgtgcagcc tggaggaagc gatccgtgtt ctgcgcgaga
      181 aaatcgattg cctgcccgga gcggtcctcc agcatgaagg tcacgccgcc ggcggtgccg
      241 atccctggaa ttgccggagg tgaaaaggcg aaggcctgcg catcaggaag cccggcaagc
      301 cgctggttca acttctggat gatcgcagct gcagtcaatc ctgagccgcg ctcgtcccat
      361 ggtttgaggg tgacgaaaaa aaatgcattg taactgatat tcgcaaggct caagaggctg
      421 aagccaacta ccgtgttgta agtctgaaca cccggagtct cacttaaaat tgcttcgatt
      481 ttttttgcga catcacccgt ccgctgtagt gaggacgctg tcggcagttg cacgttcatg
      541 tagaagtagc cctgatcttc ctcagggata aaaccagtcg gcaggcgcga gccaacgaaa
      601 ccggccgaaa ctgcgatgac aagcagcagc aagagcgaga cggcaacttt atggagcaga
      661 acatcgctgc cgaccaggta gaaatgcatg accttgccga atcccgagtt gaacgaattg
      721 aagaatctgc cgaggaggcc gcttgattcc ttacccggct tgaggagcag cgccgaaagc
      781 gcgggcgaaa gcgttagcgc gttaaaggcc gagaacagtg tcgagaccgc aatcgtcacg
      841 gcgaactgat tattgagtcg tccgcttatt cctccgacga atgcaggggg caaaaacacc
      901 gccgcaagaa ccaatgcgat tgccacgatc ggcccggcaa cttcttccat cgctttgagc
      961 gtcgcgtcgc gcggcgaaag ccccttctca atatgctgtt cgacagcctc gaccacgacg
     1021 atcgcatcat ccacgaccag accaatggcc agaacgagac caaacagcga cagagtattg
     1081 atcgagaaac caagaaaagg gaaaaccgcg aacgtgccca ccagtgaaac cggcaccgcc
     1141 agcagaggaa tcagagtgga ccgccagttc tgcaggaaga tgaatacgac gaggaccacc
     1201 agaatcagcg cctcgacgag agtagtgacg atctctttga tgccttgcga tactgcaagg
     1261 gtcgtatcca gcgagacgat gtaatcgagg tcttcgggaa atctggtttt caattcttcc
     1321 atcaacgcct tggcgctttt cattgtgtcg agcgcattag tgtcgggaag ttggtacacg
     1381 gcgatgatcg ccgaggggtt tccgttcagg cgtccgatct ggctgtaact ctgtgcccct
     1441 aactccacgc gagcgacatc cttcatccgg acaaccgaac cgtccggatt ggcgcgcacg
     1501 accacctcgc caaattcttc cgcagtcaag aggcggccct gcgcgcgaac tgtataggtg
     1561 aactgctgtc cgcgaggcac aggctcgccg ccgatctggc cggccgggtt gaccttattt
     1621 tgattggtca gcgccgtgat aatgtcggtg acggttatcc ccatgctggc gagcgtgtcg
     1681 ggacgcaccc agaagcgcat ggcgtattgt ccggcaccga agatcagcac ctgtccgatt
     1741 ccaggcacgc ggctcatagg gtcgtagatg ttgatgtaag cgtagttggc gagaaacgtt
     1801 gcatcatacg ttcccttggg cgaatagagt gaaaacaatg ctagtggact ggtcggtgat
     1861 ttccggttcg tcacgccata gttgcgcaca tcctgaggca aataggactc cgcctgcaaa
     1921 tagcgtaagt gggtcaaaat ctggtcatca ttcggtttgg tgtcaacatc gaaatcgacc
     1981 cgtagtcgca tctgtccgct attgtcgttc acggaaatca tgtagagcat gttgtcgacg
     2041 ccctgcattt gctgctcgat tggtgtcgca accgattgct caaccgttag agcatccgcg
     2101 ccgacgtaat tggtttgaag gagaatttcg ggcggtgcga ggttggggta ttgctcgatc
     2161 ggcagccgag cgatggcgac gataccgacg atcaccatca ggatggcaat gacgattgca
     2221 atgatagggt gatagacgaa aaatctggac atagcggtta cctcgtcggc gctgctggcg
     2281 caggctgcgc ctccgccgtt gttgtgagcg gcttcgggtt aaccgtcagg ccgccacgca
     2341 ccttctggac tccctcggcc acgactcgct cgccgggctt caatccctgc tcgataatcc
     2401 actccgtgcc aacctgttcg ccaaccttaa ccggacggat ttgaactttg ttatcgctgc
     2461 caacgacagc cacctgatag ttaccctgca attcactaac ggctttctga ggaaccagca
     2521 gcgcgcctct gcgtgtcgct ataattgtgc gaacgcgggc gtactggccg ggacgaagca
     2581 catttccggg gttcgggaag agaccctgaa cgcgcagtgt accggttttg acatcgacct
     2641 ggcggtcaac caaagtagtt tgcccttcgt ggggataaac caacccattg gccaaaatca
     2701 attgcaagcg caaatggcgc ttatcttcat cggggtgttc tttaacgtag tccagatatt
     2761 cccgctcgct cacgggaaag tagattttaa tgggatcaac ggtggatacg gttgtgagta
     2821 gagtgctggg ggttactaga tcaccaagct gtccctgggc aatcccggca ataccgtcga
     2881 tgagggaaag aactctggta aagccgaggt tgagctgcgc tgatgccagt tgccccttcg
     2941 ctgcttcgat ctgtgctttg gccgcgtcta cctgagcctt ggcccccaga ttcacctgga
     3001 ttgcggtgtc gagatcttgc tggggaatgg cttgctcttt ggctaggggc gtgtagcgat
     3061 tcacgtcatt ctgcgttttg acctgattcg cctgcgcact ggcaagcagc ccttccgcat
     3121 tggcgagttg acccttgtat tgatcaaccg ccgcctgaaa agttcgcgga tcgatctcga
     3181 agagcaactg gtccttttta acgaacgcgc cgtccttata agtttggcgc agcaggtagc
     3241 cggtcacctg cggcctgatc tgcgcgttcc acagtccgtc caacgtgccg atccactcct
     3301 tccagatcgg aacgtccttt tggagcacct ccaccacttc aacgtccgga gttgtgctcg
     3361 ctggctgctg tgccttttgg caaccgatca gagagaggat tgggttgaag atcaaaaacg
     3421 cggcgacaaa cagtcggagt ttttcaaaag gtaagcacat ggggtgtaac acgagaatac
     3481 tttcgttgac gcggcgtaac aaagggtgtc ataacacccg cgctgtttcg ggtcaacatt
     3541 cttggaccgg gtttcgcttt gtgataggac acgacgtgaa ttatggatga ttccgtcttt
     3601 acacggcggt ggattgtgcg aaagtttcgc tcgcaacgat gacgacagcc ggtcatgcga
     3661 cgctttgaac tagtggaacc caggactctg gaagaggcat gcggtctact gagcgacaac
     3721 aatgaagcca gggccatcgc cggcggcacc gcgctcctca ccctcatcaa acacggggtt
     3781 tttctaccga agactctcgt caatctaaag aaaataaaag gcgcgaccga gatcgactac
     3841 gattcacaaa gaggactgcg catcggcgcg ctcgcgacta tttacgacgt ggagagttca
     3901 ccgctggtac agcggcatta tcccgtgctg ggggcagcgt gtcatgtggt cgccaatatt
     3961 cgaatccgca acgtggcgac catcggcggt aacttggccc acggtgacta tcaatcggat
     4021 ccgccgacgg tgctggtcgc gctcgatgcc ggcctggaac tcatgcgccg aaatgcaacg
     4081 cggcagatca aaatagccga ctttttgcgc ggcagctatg agactgcact ggaaccaggc
     4141 gagcttgtag cagcgatact gattcccccg gctgccgatc tcaaaggtgc ttacatcaag
     4201 ttcacgaccg gctcttcgga ggagcggccc tgcgcgggaa tcgccgtcct cgcgaaaatg
     4261 gaaaatagca cctgtcgatc gttggctatc gttgtcggtg ccgtatcgcc gcgaccggtg
     4321 cgcattgaca aagcggagga gatggctagc ggtcaaaggc tcgatgtgaa actcatggaa
     4381 caaatcgcca ccgaggcgag ccgcgcagtg gatccgatcg acgatcttcg cggttcggcc
     4441 gattataaac gccacctcgt ccaggtgctc gttccccgcg ctctatccac tgtcgcgcaa
     4501 acctaagagg aagacgcaac aatggctgtg gttggaacta atgtaacgat ggtgggaggt
     4561 ctagccaaag ttaccggcgc ggtcaattac gctccggatc tggtgctgcc gcggatgctc
     4621 tacgctaaag cgctgcgcag tccatatgct cacgccgatc tactccgagt cgatgccagc
     4681 aaggcagaga aattgccggg cgtcgtcgcc gtgatcacgc gcgacgatct ggccggcttg
     4741 agtccttatt tcgggccggt ggtcgatgac cagccggtcg tggctacgga t
//
LOCUS       DASSWZ010000065         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1105733, whole genome shotgun sequence.
ACCESSION   DASSWZ010000065 DASSWZ010000000
VERSION     DASSWZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105733"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFN58_01520"
     CDS             complement(<1..284)
                     /locus_tag="VFN58_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916696.1"
                     /translation="MTKHIEIAYKEHGYEVRPTAEHPSFQSCHDRPQAEETKKKDFPL
                     KNSGLTSEIKARILGISLVILWLLVWEWSVQSGLLRNFFLQPSAILALLYR"
     gene            complement(507..1235)
                     /locus_tag="VFN58_01525"
     CDS             complement(507..1235)
                     /locus_tag="VFN58_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HET8916697.1"
                     /translation="MLLLSLIFLVAIPLFMVVSGQAQLGRDYRRADRSSAGIAPPAES
                     TPEAVVQVYAARALNWRGIFGVHTWIATKPENATEYTLHQVIGWRTYRDLPAVVSAPG
                     IPDGRWFGNEPELVGDLRGEAAAKAIPKIIEAVASYPYPNEYTLWPGPNSNTFTAHVG
                     RQVPELRMDLPTTAIGKDYPINGSLVDRTPSGTGYQFSMLGVLGLTLAREEGIEMNLL
                     GLSFGIDFLRPALKLPFIGRLGVS"
     gene            1568..2050
                     /gene="ispF"
                     /locus_tag="VFN58_01530"
     CDS             1568..2050
                     /gene="ispF"
                     /locus_tag="VFN58_01530"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET8916698.1"
                     /translation="MAREFRIGHGFDVHRFRRGRKLILGGVEIPYRMGLAGHSDADVL
                     CHAVINAVLGAMSEGDIGMHFPDTDPRYRGIASTKMLDTVLRIMRRRHFKLVNVDVTL
                     VAQQPKLAPYYAAMKKNIARRFRVAESKISIKATTTEKLGWVGQGKGMAATAVVLLRT
                     "
     gene            2119..2466
                     /locus_tag="VFN58_01535"
     CDS             2119..2466
                     /locus_tag="VFN58_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916699.1"
                     /translation="MTSDPPNGAQEAREQEIERLAQEIGHLIQGANPERRDDLKELAF
                     ALIREELIHKVEGEESGGGSTPAPFNPLGTGILIFVLGAGLSFVFGPVGLVLMFGGLI
                     FVVWGALISWFKG"
     gene            2474..2992
                     /locus_tag="VFN58_01540"
     CDS             2474..2992
                     /locus_tag="VFN58_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8916700.1"
                     /translation="MAEAEKKKKGIWSRRDFFTRLGWGGFGIFGGLTLLGFLRSAFPR
                     VLFQPPSTFKAGLPTDFAIGEVSEKFKQDQRVWIVREEVGIYAIFAKCTHLGCTPRWL
                     AAENKFKCPCHGSGFFKSGLNFEGPAPRPLDRFKVTVGDDGQLVVDKARVFKMGPGAE
                     PDEQYADSVLKV"
BASE COUNT          720 a          748 c          883 g          727 t
ORIGIN      
        1 cgataaagga gggctagaat agcgctcggc tgtagaaaga aatttcgcaa cagaccagat
       61 tggacgctcc actcccacac caacagccac aggattacca ggctgatgcc caggatgcgc
      121 gccttgatct cgctcgtcag accggagttc ttcaagggga aatccttctt tttagtctcc
      181 tcagcctgcg gcctatcgtg gcatgattga aagctcggat gctccgctgt cgggcgcacc
      241 tcgtaaccgt gttccttata ggcgatctct atatgctttg tcacctgctt ttttcgaaga
      301 tctgctgccc gaattgcatc aacaggcagt cgcagtacag cgatgccgtg cggtaggctc
      361 gcgattcgaa ttctgaataa ttgcaagctt cgattttttt aacccgaaaa aaacaggtga
      421 gtgatagggc cattcgaatt ttcgaggaga gctcgtgcga atgtaggacg caagaaatgg
      481 ccgctgcagt gggtttggga gtagttctag ctaacgccga ggcgaccgat gaacgggagt
      541 ttcagggccg gccgcaagaa gtcaattccg aaacttaaac cgagcaagtt catttcgatt
      601 ccttcctcgc gcgccaaggt caggccgagg acaccgagca tcgaaaattg atagcccgta
      661 ccgctgggag tgcgatccac cagggatcca ttgatcgggt agtctttgcc gatggctgtg
      721 gttgggaggt ccattctcag ctccggaacc tgtcgcccaa catgggcggt aaaggtattg
      781 ctattggggc cgggccagag tgtgtattcg ttagggtagg ggtaacttgc aacagcttcg
      841 atgatcttcg gaatggcttt tgccgcagcc tcgccacgca agtcaccgac cagctccggt
      901 tcattgccga accatctacc gtccggaatg ccaggggcgg aaaccacagc gggtaggtcg
      961 cggtaggtgc gccaaccgat gacctgatgc aaagtgtact cggttgcatt ctcaggtttg
     1021 gtggcgatcc aagtgtgcac gccgaaaatg ccgcgccagt ttaaagcgcg ggcggcatag
     1081 acttgaacca cagcttccgg ggttgattca gcggggggcg cgatgccggc actggagcgg
     1141 tccgctctac ggtagtccct tcccaattgc gcttggccgg aaacgaccat gaacaaaggt
     1201 atcgccacga gaaagatcaa agataacaga agcattcggg tcaactggat tctcctactt
     1261 cgctcgggaa gtatagccca cggatgagcg caactccaga cagccgactt gcaaagtgat
     1321 ctgcggcgtt attgattgtt ccttcggatc gcgtgatgat gccactgtga gtaccgtcgc
     1381 ataatcattt tgtaaagaca atggaaagat aaccgtgtcc atagccacac ttaaatacgt
     1441 tttgctgtgc tgggcacaag ggctgagtgt cgttggcggg gaccagaaaa cacttagggg
     1501 aagggtcact gttgcccgtg actccgctca atcattcgga tctggtcccc aaccaggttt
     1561 ctgaagaatg gctcgagaat ttcgcatcgg acatggtttc gacgttcatc ggtttcgccg
     1621 cgggcggaaa cttattctcg gcggtgtgga gattccctat cggatgggtc ttgcgggtca
     1681 ctcagatgcg gatgtgctgt gccatgctgt gatcaacgcc gttctcgggg cgatgtctga
     1741 aggggatatt ggaatgcatt tcccggacac cgatccccga tatcggggaa tcgccagcac
     1801 aaaaatgctg gataccgtct tgcgcatcat gcggcgcagg cactttaaac tggtcaatgt
     1861 ggatgttact ctggtggcgc agcaacccaa actggcgcct tactatgccg ccatgaaaaa
     1921 aaatatcgcg cgacggtttc gggtcgcgga aagcaagatc agtatcaagg cgacgactac
     1981 cgagaaactt ggatgggtgg gccaaggtaa aggaatggcc gccaccgcag tcgttttgtt
     2041 acgtacataa atgagcgtta cgcagtgaaa aggttgacaa taaatcattt ctatattaat
     2101 caaccaatct aatcttaggt gacatccgat cctcccaatg gcgcgcagga agcgcgtgaa
     2161 caagagatcg agcgattagc tcaagagatc ggccatctca tccagggagc caacccggaa
     2221 cggcgggacg atctcaaaga gttggctttt gcccttatcc gcgaggagtt gatccacaag
     2281 gtggagggtg aagaaagcgg tggagggtca actccggccc cattcaatcc gctgggaacg
     2341 ggaattttga tatttgtttt aggggctggt ttgtcttttg tttttggacc ggtggggttg
     2401 gtattgatgt ttggagggct gatcttcgtt gtttggggcg ccttgattag ttggtttaaa
     2461 ggatagagtg ctcatggcag aagcagaaaa gaaaaaaaag ggtatctggt cacgcaggga
     2521 tttttttaca aggctcggct ggggaggctt tggtattttc ggtgggttga ctctcttggg
     2581 ttttctcagg tcggcatttc cgcgagttct ttttcaaccc ccgtcaacct tcaaagccgg
     2641 attgccaacc gacttcgcta tcggcgaagt cagcgaaaaa ttcaagcagg atcaacgcgt
     2701 ctggatcgtt cgggaagaag tcggcatcta cgccattttt gccaaatgca cccatctggg
     2761 ctgcacgccg cgctggctcg ctgccgagaa taaattcaaa tgtccgtgcc acggtagcgg
     2821 cttctttaag tcgggtttga atttcgaagg gccggcaccg cgccccctgg accgctttaa
     2881 ggtcactgtt ggcgacgatg gtcagcttgt cgtcgataaa gctagagtct tcaaaatggg
     2941 tcccggagct gagccggatg agcagtatgc cgacagcgtc ctgaaagtct aacggatagg
     3001 aatcatgagc cttttagata agatcaaaag cgatatcgtc gaaagccacg tttggaaggc
     3061 gattttccgc cacggcta
//
LOCUS       DASSWZ010000066        13546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_808460, whole genome shotgun sequence.
ACCESSION   DASSWZ010000066 DASSWZ010000000
VERSION     DASSWZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808460"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFN58_01545"
     CDS             complement(<1..426)
                     /locus_tag="VFN58_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HET8916701.1"
                     /translation="MRHLPFEDLGFAKVDHHRTLRQGFPEVIMGQGKQPKEIAAIVRA
                     LRRRRANVLVTRLSAEKMAMVKKLVSGLKYHAAAHAATCIGKPIKITGKGAVLVVCAG
                     TSDIPVAEEAALTAAMMGNRVEKLFDVGVAGIHRLLGNRQ"
     gene            complement(550..1542)
                     /gene="thiL"
                     /locus_tag="VFN58_01550"
     CDS             complement(550..1542)
                     /gene="thiL"
                     /locus_tag="VFN58_01550"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET8916702.1"
                     /translation="MKLSRLGEFGLIERVRRTTAQGRGVNLGIGDDAAWVQTRFGSCL
                     LTADLLIEGVHFDFKWTSLYALGYKTLAVNMSDIAAMGGTPAYLLLSLGIPADFESED
                     LEEFYRGIRSLALKSGIALVGGDTSVAKSLFISACLVGQVPCRPITRSGAQAGDDIYV
                     TGTLGDSALGLKLLKQKSPKLTQRAAKFLLSRHHFPTARVRTGATLARENLARAMIDI
                     SDGLLQDLGHVCKASRVGAIVAEEKLPLSVAYRSLTGHDGTDLALAGGEDYELLFVAR
                     RRDRSRIEKLQEFVDVPISRIGTCVRPRDGIAVLNRKGQRVSFRATGHDHFKHL"
     gene            complement(1628..4135)
                     /gene="lon"
                     /locus_tag="VFN58_01555"
     CDS             complement(1628..4135)
                     /gene="lon"
                     /locus_tag="VFN58_01555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET8916703.1"
                     /translation="MSEAEWKIEGQEGKASQVEIPEVLPLLPIRDIVIYPYMMLPLFV
                     GRDVSIRAVEESLSRDRLIFLVAQKNSAEEAPSPSDINRVGTIASIMRMLKLADGRVK
                     ILVQGLSKGEVDTYLRERPFFEVKIRKVIEPTLPEVPIEVEALMRTAKEKIEQILNLK
                     NLPPEIVMVTDNISDPGVLADLVASNLRLKIEESQAILEIFDPIERLKKVNELLSREL
                     ELSTMQARIQNQAKEEMSKTQRDYFLREQLKQIQQELGEGDERTEEINELKKQLDKAK
                     MPVDVKREADKQLKRLEQMHPESSEASLVRTYLDWLVDLPWGKKTKDNLNIKKAKQVL
                     DEDHYNLEKIKERILEYLAVNKLRRKIKGPILCFVGPPGVGKTSLGRSVARALGRNFV
                     RISLGGVRDEAEIRGHRRTYVGALPGRVIQGIKQAGSNNPVFMLDEIDKVGADFRGDP
                     SAALLEVLDPEQNHAFSDHYLNLPFDLSNVLFICTANMLDPIPPALADRMEVIQLSGY
                     TNEEKLEIARKYLLPRQLEDNGISTKYLEFSDDAVLRIIAEYTKEAGLRNLEREIASI
                     CRKVARKIAEEKKDLTRITRANLHSFLGAPKFSPETEQEHHEIGVATGLAWTSAGGEL
                     LFVEASLSKGRGNLTLTGQLGDVMKESAQAAVSYARAHARKLGIEEDFYQKLDIHIHV
                     PAGAIPKDGPSAGITVATALISALMKRPVSRDVAMTGEITLRGRVLPIGGLKEKSLAA
                     FRAGIKTIVLPDRNEKDLDEVPKSLRRRLRWIVVKNMSDVLKIALLPEQSRARRAASA
                     VASKTKLRTRKGGPWKRSERPLRKNQRERSIPLLPSH"
     gene            complement(4259..5365)
                     /gene="dnaJ"
                     /locus_tag="VFN58_01560"
     CDS             complement(4259..5365)
                     /gene="dnaJ"
                     /locus_tag="VFN58_01560"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET8916704.1"
                     /translation="MSEKNDYYDLLGVNRNSSEDEIKKAYRKLALKYHPDRNPGDKQA
                     EEKFKEVSEAYQVLSEPQKRAQYDQFGHAAFGDGGPFAGGFDFTAGFEDVFGDIFGEF
                     FGGTRRRSRVRGEDLRYNLTLTFEEAISGTEKKVKIPRQGPCESCHGNGAKPGTVPQS
                     CPTCRGRGQVSFQQGFFSVSRTCNQCHGHGTIIKDPCTTCGGAGRVRKLHTLSIKIPT
                     GVDTGSRLKLRNEGESAPTGGAPGDLYVVIQVEPHPIFVRENLDIVCDVPISFVHAAL
                     GAEIDVPTLDGKVKMKIPAGTQSGKLFRMKGKGVKDVQGYEQGDQHVRVVVETPTHLT
                     PRQKELLKEFAVSGGEEVNPLSKGFFDKVKELFG"
     gene            complement(5491..7410)
                     /gene="dnaK"
                     /locus_tag="VFN58_01565"
     CDS             complement(5491..7410)
                     /gene="dnaK"
                     /locus_tag="VFN58_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET8916705.1"
                     /translation="MAKVIGIDLGTTNSCVAIMESGDPTVLANSEGSRTTPSVVAFTD
                     NGERLVGQIARRQAITNPENTIFAVKRLIGRRSDDPMVQKAAKVLPYKVVRNENGDAW
                     VEIRGKQYSPAEISAFILQKMKQTAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLNVLRIINEPTAASLAYGLDKKKDEKIAVFDLGGGTFDISILELGDGVFEVKATNG
                     DTFLGGEDFDQRIIDYLADEFKKDQGIDLRKDRMALQRLKEAAEKAKCELSSSMETDI
                     NLPFITADQQGPKHLNMKLTRSKLEALCADLIDRTEGPCRQALKDSGLSPSDINEVIL
                     VGGMTRMPAVQERVKKIFGKDPHKGVNPDEVVAVGAAIQAAVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQIFSTAQDNQTAVSIRVFQGEREMAANNKLLG
                     QFDLVGIPVAPRGVPQIEVTFDIDANGIVHVAAKDLGTGKEQSIKITASSGLSEDEIK
                     QMVKDAEAHAAEDKKHKETVEARNQLDSLIYSTEKSLKEYGNDLDAGVKENIENALKK
                     ARETLEGQDAQAMRTAAEQLSQSSHKLAEAMYAKASRQQPGQEASGDAKEQPTSDGKK
                     KKDDVVDADFEEVKD"
     gene            complement(7460..8086)
                     /gene="grpE"
                     /locus_tag="VFN58_01570"
     CDS             complement(7460..8086)
                     /gene="grpE"
                     /locus_tag="VFN58_01570"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET8916706.1"
                     /translation="MADEKEKIGAHATTDGLDPSVSEAQSTALDQAAELAKLRENLEA
                     KEKEANENYDRYLRQVAELENFRKRANRERDEAIRFANEALVKDLLPVVDNLERAVVH
                     AKDGGNGKPLVEGVEMVLRGFFDTLAKHGVVPIAAVGQRFDPEKHEAMAQIESGTYEP
                     DTVVEEYHKGYLLRDRLLRPALVSVAKTPKSQGKKNNGGEVENEPGDD"
     gene            8233..9000
                     /gene="recO"
                     /locus_tag="VFN58_01575"
     CDS             8233..9000
                     /gene="recO"
                     /locus_tag="VFN58_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET8916707.1"
                     /translation="MVLCHVTPAIVLRTWPFGESDKIVSFLTENHGKLTGIAKGAKRS
                     RKRFVNSLEPFSLVNLRFQDRAHSNLAFIVAADLTLGFKHLLTSLEKISFASYLVEIT
                     DGLIGEREENRLVFLHLKAGLTHLEEQSTSLTFLTAFELKLLKLAGYQPVLESCRRCG
                     RERWDRCATRWHFSPRDGGILCESCSQLRKEIFSLSAATLEILTNIQDEKKILSSDVL
                     PPTRVLKEIRSVVVRFIEFQMEREIKSAAFLNQFALA"
     gene            9068..11788
                     /gene="ppdK"
                     /locus_tag="VFN58_01580"
     CDS             9068..11788
                     /gene="ppdK"
                     /locus_tag="VFN58_01580"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET8916708.1"
                     /translation="MLKKKKVVYFFGGGKAEGRAAMRELLGGKGAGLHEMTRIGAPVP
                     PGFTITTDVCGYYYAHHQQYPKGLASDVIAGLARIEKILGRRFGDSDNPLLVSVRSGA
                     RESMPGMMDTVLNLGLNDRTVQGLIRRTQNPRFAYDSYRRFVQMYADVVLGLKPRERT
                     ERDPFEEILEQRKKTRHVRFDTELEAKDLEAVVTAFKELVKSRTGKEFPDDPQEQLWG
                     AIGAVFGSWNNDRAVAYRELYHIPDTWGTAVNVQAMVFGNLGEKCATGVAFTRNPSTG
                     EKSFYGEFLVNAQGEDVVAGIRTPRPIAELKMVMSRPYTELQKVCKILETHYKEMQDI
                     EFTVEDNKLWMLQTRSGKRTGFAAVRIAVDMVEERIISKKEALLRVEADHLNQLLRPI
                     FDNKDKEQAAREGRILAKGLPAGPGAATGKIVFHAEDAVAWQQSGEQVVLCRTETSPD
                     DIRGMEAAQGILTARGGMTSHAALVARQMGKVCVAGCEALSIDYSARMMRVGETVLHE
                     GDWLSVDGSTGEVIQGEIKTRPSEVLQVLVGGTLEAKKSRVYQQYARLMKWADEVRTL
                     GVRTNTDQPDQARVAKAFGAEGIGLCRTEHMFFQGDRIQAVREMILADDEAGRRRALA
                     KLLPMQKEDFKGILDIMGNLPVTIRTLDPPLHEFLPHRDDEIIELAQVMGVSVDRLRD
                     KVKVLQEFNPMLGHRGCRLGISYPEITEMQARAIFEAACELRKEGNNPFPEVMIPLVG
                     SRGELIAQRRIVEQAANETMASYGVKVRYLIGTMIELPRACLVADEIAEEAEFFSFGT
                     NDLTQTCLGLSRDDAGKFLPTYVLDGLLADDPFVSVDRDGVGALMRIAITKGRKVRRN
                     LEIGICGEHGGDPRSVMFCHEIGLDYVSCSPFRVPIAKLAAAQAALRGKP"
     gene            11906..>13546
                     /gene="pilB"
                     /locus_tag="VFN58_01585"
     CDS             11906..>13546
                     /gene="pilB"
                     /locus_tag="VFN58_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET8916709.1"
                     /translation="MEPRIAELLVRGGIVSRDQLVQAQEKERDNGSSVIRELIRLGFT
                     TEDTLTEFLAKQFSIEKVDLSPSEIEDSVFSLVPPQLVQKHQLVPVKLLGSTLTVAMA
                     DPTDLVAINEVKFITGYAVRVTLAPPSAIKKTLDHRFGGVSYDEVLKKFGDGEMEVIH
                     ETDDVNLQELQQATMEAPVVTLVNAILADAAKRRASDIHIEPYEKIFRVRFRVDGVLQ
                     EIMSPPLRLKNPLVSRLKVMAGLDIAERRLTQDGRIKLKMGVGGELDIRVSVLPTLFG
                     EKVVMRLLDKSNLQLDMSKLGFDPQTLKDFLEAIHKPYGMILITGPTGSGKSTTLYSA
                     LSELNKPDVNISTAEDPVEYNLVGINQVQVREQIGLTFAAALRSFLRQDPDIIMVGEV
                     RDLETAQIAVKAALTGHLVLSTLHTNDAPSTVDRLINMGVETFLLISSINIIAAQRLV
                     RRICEKCKESAEVSPEILINLGVDSAEVGTGFPTFHGRGCANCNGTGYRGRLAIYEVM
                     VMHEVLKELILRNASVVELKREAVKQGMSTLRMSALQKV"
BASE COUNT         3067 a         3522 c         3555 g         3402 t
ORIGIN      
        1 ctgccggttt cctaggagac ggtgaattcc tgccacaccg acgtcaaaaa gcttttcgac
       61 tcggttgccc atcatagcag ccgttagggc ggcttcttca gcgacgggta tatcggaagt
      121 gcccgcacag acgaccaaca cggcaccctt tccggtaatc ttaatcggct tgccgatgca
      181 ggtcgcggcg tgcgccgcag catggtattt gagcccggag accagctttt tgaccatggc
      241 catcttctca gctgataatc gggttacaag tacatttgct cttcgacggc gcaaagcacg
      301 tacaatggct gcgatctctt tcggctgttt gccttgtccc atgatgactt cgggaaaacc
      361 ctgacggagg gtgcgatgat ggtcgacctt agcaaagccc aggtcttcga aagggaggtg
      421 gcgcaaacgc gctaccgcct gcgatacgct aacttttcct cgccgaactt ttgttagaag
      481 ttctacaatt cgttccgagt ccattcagac cttgattaat tacggtgcaa tttgtgaata
      541 ctctcagagc tataaatgct taaagtgatc gtgaccggta gcgcgaaagg aaacccgttg
      601 acccttgcgg tttaagacag ctatcccgtc gcgtggacgg acacaggtac cgatacggct
      661 aatcggaaca tcgacgaact cctgaagctt ttcgatacgc gagcgatcgc gtcgccgggc
      721 aacaaagaga agttcatagt cttccccgcc agcgagggcg aggtccgtcc cgtcatgtcc
      781 agtaagggag cggtaggcta ccgagagcgg tagcttttct tctgcaacga tagcgcctac
      841 acggctggct ttgcagacgt ggcctaagtc ctggagcagt ccatcactga tgtcgatcat
      901 tgcgcgagcg aggttctctc tcgctaatgt cgctccagtt cggactctag cggtcggaaa
      961 atgatgtcgc gagagaagaa actttgccgc acgttgtgtg agcttgggcg acttttgttt
     1021 gagcagcttc aagcccagag ccgagtcgcc tagtgtgccg gtgacgtaga tgtcgtcgcc
     1081 tgcttgtgcg ccgctgcgag taataggacg acaaggcact tggccgacca agcaggctga
     1141 aataaataaa gacttcgcga cgctggtatc tcctcccacc aaggcgatgc cgcttttcag
     1201 agccaaagag cgaatccccc gatagaactc ctccaaatcc tcactctcaa agtcggcagg
     1261 aattccaaga gagagaagca agtaagccgg cgtacctccc atagctgcaa tgtcactcat
     1321 gttgacggcg agagtcttat aaccgagggc gtatagggac gtccacttaa aatcgaaatg
     1381 gacaccctcg atcagaagat ctgcagtcaa caggcatgaa ccgaacctcg tctggaccca
     1441 ggccgcatcg tcgccaatgc ctaggtttac gccgcgtccc tgagccgttg tacgccggac
     1501 gcgttcgatc aagccaaatt cgcctaagcg actgagtttc attgagtgat ggccgctaca
     1561 ctcgatgggt caaaccccat tgtagttcag tcgaggcaga ccacagcagt tttcggaccc
     1621 gacgatttta gtgactgggt agaagcggaa tggatctttc tctttgattc tttcgtaacg
     1681 gtctttcact gcgcttccac ggccctccct tgcgtgtcct aagtttcgtc ttggaagcga
     1741 cggcagatgc agcacggcga gctcgacttt gctctggcaa caatgcaatt tttagcacgt
     1801 cagacatgtt cttaacgacg atccatcgaa gcctgcgccg gagcgatttg ggaacctcat
     1861 caagatcctt ttcgttccta tctgggagga caatggtctt aatgccggcc cgaaaggcgg
     1921 ccaaggattt ttccttgagc ccgccaatgg gcagtactcg gccgcgcagc gtaatctctc
     1981 cggtcatcgc cacgtcccga cttaccggcc gcttcatcag agcggagatc agagccgtcg
     2041 ccacggtgat tccggctgag ggtccgtcct tagggatggc tcctgctggg acatggatgt
     2101 ggatatcgag tttctgataa aaatcttcct cgatacccaa cttgcgcgcg tgagcgcgag
     2161 cgtaactcac cgctgcttga gcggactctt tcatgacgtc gcctaattga cctgtcaagg
     2221 tcaaatttcc tcgccccttt gatagggagg cctcgacaaa caaaagctca ccgccggcgc
     2281 tcgtccaggc cagcccggtg gcaacgccga tctcatgatg ttcctgttcg gtttccggcg
     2341 aaaatttcgg cgcacccaaa aatgagtgaa ggttggcacg ggtgatacgg gtgaggtctt
     2401 ttttttcctc ggcaatcttc cgtgcgacct tccggcaaat agaggcgatc tctcgctcga
     2461 ggttccttaa accagcctct ttggtgtact cagctatgat gcgcaacacg gcgtcatcgg
     2521 agaactcgag atatttggta gagatgccgt tgtcctcgag ttgtcttggg agcaagtatt
     2581 tgcgggcaat ctccagtttt tcctcgttag tgtaaccaga gagctggatg acctccattc
     2641 gatccgccaa cgccggtgga atcggatcca gcatgtttgc ggtacatatg aacagaacat
     2701 tggaaaggtc gaacggcaaa ttcaggtaat gatcgctgaa ggcatggttt tgctcggggt
     2761 ctaaaacctc aagcagcgcc gccgaaggat cgccacggaa gtccgctccg actttgtcga
     2821 tttcatccaa cataaatacc ggattgttcg atccggcttg ctttattccc tgaatgacac
     2881 gaccgggcaa agcgccgaca taggttcgtc gatggccgcg aatctctgcc tcatcgcgaa
     2941 caccgcccag tgatattcgg acgaagtttc tccccaaggc gcgggcgaca gatcttccca
     3001 gggaagtttt tccaacaccc ggcgggccaa caaagcagag aatagggccc ttaatcttcc
     3061 gtctcagctt gttgaccgcg aggtattcca aaatccgttc tttaatcttc tccaggttat
     3121 aatgatcctc atcgaggact tgttttgctt tcttaatatt cagattgtct ttggttttct
     3181 tgccccaagg caggtccacc agccagtcaa ggtaggtgcg caccaaagat gcttcagaag
     3241 attccgggtg catttgctcc aatcgcttga gctgtttgtc tgcctcacgc ttcacgtcca
     3301 cgggcatctt ggctttatcg agctgcttct ttagctcgtt gatttcctca gtgcgctcgt
     3361 cgccctcacc cagttcctgc tgaatctgct tcagctgttc gcgcaggaag taatccctct
     3421 gggttttgct catctcctcc ttggcttgat tttggatccg cgcctgcatc gtcgacagct
     3481 ctaactcgcg gctaagaagt tcgttgactt tttttaaacg ttcaatggga tcaaaaattt
     3541 caaggatcgc ctggctctcc tcaattttga ggcgcaggtt tgacgccact aggtccgcca
     3601 gtacgccagg atcacttatg ttgtccgtca ccatgacaat ctcaggcggt aaatttttga
     3661 gattcaagat ctgttcaatc ttttccttag cggtccgcat caaggcttcg acctcgatgg
     3721 gaacctccgg cagcgttggt tcgatgactt tgcggatctt gacttcgaag aaaggtcgct
     3781 cgcgtaagta ggtgtcgact tctccctttg acaaaccctg gacaagaatc ttgacccgcc
     3841 cgtccgcaag cttgagcatg cgcatgatcg aagcgatagt cccgacgcgg ttgatgtccg
     3901 acggacttgg ggcttcttca gcggaatttt tttgcgcgac caggaaaatg agtcggtcac
     3961 gggaaagaga ctcttcaacg gcgcggatcg acacgtcgcg gccgacgaac agcggcaaca
     4021 tcatgtacgg gtaaatgacg atgtcgcgaa tcggcaagag gggcagcacc tccggaatct
     4081 ctacttgcga tgctttgccc tcttggcctt caattttcca ttcagcttca ctcatagttt
     4141 tggatcacct taatcacact gggactagag gtcaactaaa atcttgcacc cgacatgtct
     4201 ttgttggagt gctggaggca aagagaacag gacagggagg ttcctcgaac tcgcatgtct
     4261 agccgaataa ttctttaacc ttatcaaaaa agcctttcga gagaggattg acttcctctc
     4321 caccgcttac agcaaattcc ttcaaaagct ccttctgacg tggcgttagg tgagtcgggg
     4381 tctccacgac tacccgtaca tgctggtcac cctgctcata tccttggacg tcctttacac
     4441 ccttgccctt catgcgaaag agttttccgg attgggtccc ggcaggaatt ttcattttca
     4501 ccttgccgtc caaagtcgga acgtcgattt ctgcacccaa ggccgcgtga acgaaactta
     4561 tgggaacgtc gcaaacaatg tccaggttct cacgcacaaa gatcggatga ggctccacct
     4621 ggatgacgac gtaaagatct cccggggcac cccctgtggg cgcgctttcc ccttcatttc
     4681 ttagtttgag ccgtgatccg gtgtcgacac cggtcgggat cttaatgctg agggtgtgta
     4741 actttcgtac gcgacctgca ccaccacaag tcgtgcacgg atctttgatg atggtgccgt
     4801 ggccgtgaca ttggttgcag gttctcgaaa cgctgaaaaa accttgttga aagctcactt
     4861 gtcctctgcc gcgacaggtt ggacaagact gcggtacggt tcccggttta gcgccgttgc
     4921 cgtggcagct ctcgcacgga ccctgtctgg gtatcttgac tttcttttcg gtgcccgaga
     4981 tggcttcctc aaaagtcaag gttaggttgt agcgaagatc ttctccgcgg acgcgacttc
     5041 ggcggcgggt cccgccaaag aactcgccaa aaatgtcacc gaagacatcc tcgaaacccg
     5101 cagtgaagtc gaatccaccg gcaaagggac caccatcacc gaaagctgcg tgaccgaact
     5161 ggtcgtattg cgcgcgcttt tgtggttcgg agagaacttg gtaggcctcg gaaacttcct
     5221 tgaatttctc ctccgcctgt ttgtcacccg ggttgcggtc cgggtggtac ttgagcgcga
     5281 gtttccggta agctttcttg atctcgtcct cgctggagtt gcggttcaca ccaagcaaat
     5341 cgtaataatc gtttttttcg ctcaagtcgg gacctcaaca aagaaaatcc gtatacgtta
     5401 gaaagcccct ctcctcaatc gacagtagga gcaggggctt tcttttgtca cgaagatcga
     5461 tcactgcgtt acttcagaga atcttaaggt ctagtcctta acctcctcga agtctgcatc
     5521 gacgacatcg tctttctttt tcttgccatc gctcgtcggt tgttccttgg catctcccga
     5581 agcctcctgc cccggttgtt gtcgcgaagc tttagcatac atagcttccg ccaatttatg
     5641 agaagactga ctcaattgct ccgcagcagt gcgcatcgct tgggcatctt gtccttccaa
     5701 tgtctcccgg gctttcttaa gcgcattctc gatattttct tttacgccgg catccaagtc
     5761 gtttccgtac tccttgagtg atttttctgt cgaatagatg agggaatcaa gttgattacg
     5821 cgcttcgacc gtctctttgt gtttcttgtc ttcggcggca tgggcttcag catctttgac
     5881 catctgtttg atctcatctt cgctcagacc gcttgacgcc gtaattttga tggattgctc
     5941 tttgcccgtg ccgaggtctt tggccgcaac atgaacaata ccgttggcat cgatgtcgaa
     6001 agtcacttca atctgtggga ccccgcgcgg cgctaccgga attccaacca gatcgaattg
     6061 tcccaaaagc ttgttattag ccgccatctc ccgctctcct tggaagacgc gaatggacac
     6121 agcggtctgg ttgtcttggg ccgtcgaaaa aatttggctt ttcttcgtcg gtatagtggt
     6181 gttgcgctcg atcaattttg taaatacgcc gccaagggtc tcgatcccta atgagagggg
     6241 ggtaacatcg agaagcaaca cgtccttgac ttcacctttc agaacagcgg cctggatggc
     6301 ggcacctacg gcgacgacct catccggatt gacgcctttg tggggatctt tgccaaagat
     6361 tttctttact cgttcctgca cggcgggcat gcgagtcata ccgcccacca aaataacttc
     6421 attgatgtcg ctgggggaga gtccagaatc cttcagggct tgtcggcagg ggccttcggt
     6481 ccgatcaatc aaatctgcac acagcgcctc aagcttggag cgagtcaatt tcatgttgag
     6541 gtgtttcggt ccttgctgat ccgccgtgat aaacggcagg ttgatgtccg tctccatcga
     6601 agaagagagc tcgcatttcg ctttttccgc cgcttccttc aagcgctgca gcgccatccg
     6661 atctttgcgc agatcaatcc cttgatcttt tttgaattca tccgcgaggt aatcgataat
     6721 tctctggtca aagtcttcac cgcccagaaa cgtgtccccg tttgttgctt taacttcaaa
     6781 gacgccgtcg cccagttcca gtatcgatat gtcgaatgtt ccgccgccaa ggtcgaagac
     6841 ggcgatcttt tcgtccttct tcttgtccaa tccgtacgcg agagaggcgg ctgtgggttc
     6901 attgataatg cgcaatacat ttaggcctgc gatgcgccca gcatctttag tggcctgtct
     6961 ctggctgtca ttaaaatagg ccggcacggt aataactgcc tcggttacct tttcaccaag
     7021 atagtcttcc gctgtctgct tcatcttctg gaggataaaa gccgaaattt ctgccggact
     7081 atactgttta ccccgaattt ctacccatgc atcaccgttc tcattgcgaa cgaccttata
     7141 gggcagtacc ttggcggcct tctgaaccat aggatcgtcg ctacgccgac caatcaagcg
     7201 ctttacagca aaaatagtgt tctctggatt ggtgatggcc tgccttctgg cgatttgccc
     7261 aaccaacctc tcaccgttat cggtaaaagc aaccacagag ggggtcgtgc gactgccttc
     7321 ggagttggcg agaacggtcg ggtctccact ctccatgatc gcaacgcagg agttggttgt
     7381 cccgaggtca atgccaataa cttttgccat cttagttact ccttaaaatt ctgtaagatt
     7441 tcgaataatt tgataaaatt taatcatcgc caggttcgtt ttcaacctcg ccaccgttgt
     7501 tttttttccc ttgcgatttc ggtgtcttag ctacgcttac cagcgcagga cgaagaagcc
     7561 tatcccgaag taggtaacct ttgtgatact cctcaacgac cgtatctggc tcgtaggtac
     7621 cactctctat ttgggccatc gcttcatgtt tctcaggatc aaagcgctgg ccaaccgctg
     7681 caatgggcac gacgccatgt ttggcgaggg tatcgaaaaa ccctctaaga accatctcga
     7741 caccttctac aaggggtttg ccgttaccgc cgtctttcgc atgcacaaca gcccgttcca
     7801 gattatccac aaccggaaga agatctttga ccaaagcctc attggcaaag cgaatcgctt
     7861 catctctctc gcggttagct ctctttctaa aattttctaa ttctgcgacc tggcggaggt
     7921 aacggtcgta gttctcgttg gcttccttct cttttgcttc aagattttca cgcagctttg
     7981 cgagctcggc tgcctgatct agcgcagtcg attgcgcctc gctaaccgat gggtcaagtc
     8041 cgtcggtagt cgcgtgtgca cctatttttt ctttttcgtc ggccatgtgt tccctcaaaa
     8101 tgtcaaaaaa atctaatcac ggtgccttcc gttgtcaagt tccggctttg taaaaagctt
     8161 cgatatttta aatagttgtc tgcatcgtca aaaagtgata attcttttaa agtaagtccg
     8221 agcacttgac ccatggttct ttgtcacgtc accccggcta tcgtgttgcg cacttggcct
     8281 ttcggggagt cggacaagat cgtatctttc ctgaccgaga atcacgggaa actgaccggc
     8341 attgctaagg gggcgaagcg ctcacgtaag cgatttgtca attctctaga gcctttttcc
     8401 ctggtcaatc ttcgttttca agatcgcgct cacagtaatt tggcgtttat tgtcgctgcg
     8461 gaccttacgc ttgggtttaa gcacttacta actagtctag aaaaaatctc gtttgcttct
     8521 tatctggtcg agatcacaga tggattgatt ggggaacggg aagagaaccg tttggttttc
     8581 ctacatttga aggctggtct cactcatctt gaagagcaat cgacgtcgct aacttttctg
     8641 accgctttcg agctcaagct ccttaaattg gctggttacc agcccgtgct agagagctgc
     8701 agacgttgcg gcagagagcg ttgggatcga tgcgcgacgc gctggcactt cagcccacgg
     8761 gatggcggaa ttctttgtga atcctgttca caattgcgga aagaaatctt ttcgctttcc
     8821 gccgcgacac tggaaattct gacgaacatc caggatgaaa aaaaaatact ctcatccgat
     8881 gttttgcctc caacccgggt gctaaaagaa attcgttccg tcgtggttcg attcatcgag
     8941 tttcagatgg agcgggagat caagtcggcg gcatttctta atcaattcgc tttggcatga
     9001 agtaggcttg gctacgtctt gccgaagtac agaagtactc tgacaaccgg gatgaggatt
     9061 gaggaccatg cttaagaaaa aaaaggtagt ctattttttc ggtggcggta aagccgaagg
     9121 tagggcagcc atgcgcgagc tgctcggcgg gaaaggagca ggcttgcatg agatgacacg
     9181 catcggggcg cccgtgccgc caggctttac tataacaact gatgtgtgcg gctattacta
     9241 cgcgcatcac cagcagtacc cgaaggggct ggcatctgac gtcatagcgg gtcttgccag
     9301 aatcgaaaag atcctcggac gacgattcgg cgactccgac aaccctttgc tggtctcagt
     9361 gcgttcgggc gcgcgcgagt cgatgccagg catgatggat acggttctaa acttaggcct
     9421 caacgatcgg acggtgcagg gattaatccg ccgcacacag aatcccagat ttgcatatga
     9481 ttcttatcgt cgctttgtgc agatgtatgc ggatgttgtc ttgggactca agcccaggga
     9541 aagaaccgag cgtgatccgt ttgaggagat attagagcaa aggaaaaaga cgcgccatgt
     9601 gcgctttgat actgagctcg aggcaaagga tttagaagcc gtagtcactg cattcaagga
     9661 attagtaaaa tcgcgcaccg ggaaagaatt ccctgacgat ccccaggaac aactttgggg
     9721 tgcgatcgga gctgtctttg gttcgtggaa caatgatcgc gccgttgctt accgcgaact
     9781 ttaccacatc ccggacactt gggggacagc tgtcaacgtc caggcgatgg tgtttggaaa
     9841 tctgggcgaa aagtgtgcca caggggtggc cttcacacgg aatccttcga ctggagaaaa
     9901 gtcattctat ggtgagtttc tcgtcaacgc tcaaggagag gatgtcgtcg ccggcatccg
     9961 gacaccgaga cccattgcag agttgaaaat ggtgatgtcc cggccgtaca cagaacttca
    10021 aaaggtctgc aagattctcg agacgcacta caaggagatg caagatatcg aattcacggt
    10081 ggaagacaac aagctctgga tgctccagac tcggagcggc aaaaggactg gttttgcggc
    10141 ggtgcggatc gcggtggata tggtcgagga gcggatcatc agtaaaaaag aagccttact
    10201 gcgagttgag gctgaccatc taaatcagct tttacgtcct atcttcgaca ataaggataa
    10261 agaacaggca gccagggagg gacgaattct tgcgaaagga ctgccggctg gaccaggggc
    10321 ggctaccggg aagattgttt tccacgcaga ggatgccgtg gcctggcagc aaagcggcga
    10381 acaggtagtg ctgtgccgaa ctgaaaccag ccccgacgat atccgcggga tggaggccgc
    10441 acagggaatt cttaccgcta gaggcgggat gacatcacac gccgctttag ttgcccggca
    10501 gatgggaaag gtttgtgtag ccgggtgtga ggcattgtct atcgactata gcgcgcgcat
    10561 gatgcgcgtg ggggaaacag tcttgcatga gggggattgg ctctcggttg acgggagcac
    10621 cggggaagtg atccagggag aaattaagac gcgcccatct gaagtattgc aggtactcgt
    10681 tggtggaacg ctggaagcca aaaagtcacg ggtctatcag cagtatgcta ggctcatgaa
    10741 gtgggcggac gaagtacgca cgcttggcgt gcgtaccaat acggatcaac ccgaccaggc
    10801 gcgggtggcg aaggcctttg gcgccgaagg cataggtctc tgccgtacgg agcacatgtt
    10861 ttttcaaggt gacaggattc aagccgtacg cgagatgatt ctggcggacg acgaagcggg
    10921 taggcggcgg gcattggcaa aattgctgcc gatgcaaaaa gaagacttca aaggtatcct
    10981 cgacattatg ggtaatcttc ccgtgaccat ccgtacgctg gatccaccat tgcacgaatt
    11041 tcttccccat agggatgacg aaatcattga gctggcacaa gtcatgggcg tttcggttga
    11101 ccggcttcga gataaagtga aggttctgca agaattcaat ccgatgttag ggcatcgggg
    11161 ctgccgtctg gggatttctt atccggagat caccgagatg caggcacggg caatcttcga
    11221 agccgcatgc gagttacgca aagagggaaa caatcctttc cccgaggtta tgataccttt
    11281 ggtgggatcc cgtggagaat taatcgcgca gcggaggatt gttgaacagg ccgccaacga
    11341 gaccatggcc tcttatgggg tcaaggtccg ctatcttatt ggaacaatga tcgagcttcc
    11401 gagggcctgc ctggtggccg atgagatcgc cgaggaagcc gagttttttt cctttggcac
    11461 caacgattta acgcagacat gcctgggctt gtcgcgggat gatgccggga aatttctacc
    11521 tacgtacgtg ctcgacggcc tattggccga cgatcccttt gtcagcgtgg atcgagacgg
    11581 agtcggcgcg ctgatgcgga tcgccatcac caaaggacgc aaagtacgcc gaaacctcga
    11641 gatcggaatc tgcggagagc atggtggtga tccaagatca gttatgtttt gtcatgagat
    11701 cggattggat tacgtaagtt gctcgccctt ccgtgttccc atcgccaaac tcgccgccgc
    11761 gcaagccgca ctgcgcggaa agccatagtt tgtaggacct ttccactctt catttctaaa
    11821 tagaagcccg ttcttttatt taaaactgtt gacagaattg cctttctttt aagataattt
    11881 acatcctcct gatagctaag gtggcatgga accgcgtatt gcagaactat tagtccgggg
    11941 tgggattgtg tcccgtgatc aactcgtcca ggcccaggaa aaagagcggg acaacggctc
    12001 aagtgtgatt agggagctga ttcggctagg gttcactact gaagataccc ttaccgagtt
    12061 tcttgcaaag cagttcagta tcgaaaaagt cgatctaagt ccttcagaaa tcgaggattc
    12121 ggtgttcagc ctcgtaccgc ctcaactggt tcaaaagcac cagttggttc cggtaaaact
    12181 tcttggttcc actctcactg tggccatggc tgacccaacg gatctcgttg caatcaatga
    12241 agtgaagttt atcaccggct atgccgtgag ggtcacgttg gcccccccct cggcgatcaa
    12301 aaaaaccctc gatcaccgct tcggcggcgt aagctacgac gaggttttga aaaagtttgg
    12361 cgatggggag atggaggtta tccacgaaac cgatgacgta aacctccagg aacttcagca
    12421 ggccaccatg gaagcgccgg tggtaacttt ggtgaatgct atcttggcgg acgcggcaaa
    12481 acgacgcgcc agtgacatac acattgagcc ctacgaaaaa atttttcgcg tgcgttttcg
    12541 agtggatggg gttcttcagg agatcatgag tccgccgctt cggctcaaaa atcccttagt
    12601 gtcccgtctc aaggtcatgg ccggactgga tatcgctgag cgccgcctca cgcaagacgg
    12661 ccggattaag ttaaagatgg gggtaggcgg tgagttggat atccgggttt ccgtactccc
    12721 gacgttgttt ggcgagaaag tcgtcatgag attgttggac aaatccaatc ttcagctgga
    12781 catgtccaaa cttggttttg acccccaaac cctcaaagat tttctggaag cgatccacaa
    12841 gccgtatgga atgattctta ttactgggcc gacagggagt ggaaaatcaa caacgctcta
    12901 ttccgcgctc tcggaactca acaaacccga cgtcaacatt tccacggcag aggatcctgt
    12961 cgagtataac ttggttggaa tcaatcaagt gcaggtccgg gagcaaatcg ggttaacctt
    13021 tgccgccgct ctacggtcgt ttcttcgaca ggaccctgac atcatcatgg tcggagaagt
    13081 ccgtgatctc gaaaccgccc agatcgccgt caaagccgcg ttgacaggtc acttggttct
    13141 tagcaccctc cataccaacg atgctccctc taccgttgat cgcttgatca acatgggggt
    13201 cgagactttc ttgctaattt cgtccatcaa tatcatcgca gcccaaaggt tggtacggcg
    13261 aatttgcgaa aagtgtaaag agtcggcgga agtgagtccc gagatattga tcaatttagg
    13321 tgtggactcg gcggaggttg gcaccggctt tcccacgttt catggacggg gctgcgccaa
    13381 ttgcaacggt accggatacc gaggacgttt ggccatctat gaagttatgg tcatgcacga
    13441 ggttctaaaa gagttgattt tgcgaaacgc gtcggtggtt gagcttaagc gagaggctgt
    13501 gaaacagggc atgagcacgc tacgtatgag cgccctccag aaggtt
//
LOCUS       DASSWZ010000067         6298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_412341, whole genome shotgun sequence.
ACCESSION   DASSWZ010000067 DASSWZ010000000
VERSION     DASSWZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412341"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..902)
                     /locus_tag="VFN58_01590"
     CDS             complement(114..902)
                     /locus_tag="VFN58_01590"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HET8916710.1"
                     /translation="MMTSRDWAWGVVPPGFKRVIDGREGLMIVREDVEKFITIEECTK
                     SSDSYDHEAGLFQGRGKLRVLELRNGDAALIRPYRHGGLFRHLLNGIFFTWPPRPFRE
                     LVITEEVRRRGIPTAEVFAACVRRIWGPFYRGWLVTRQLKGGQDLWTAIRDGFVGQTG
                     AEKVFNAVAASLRGLHREGVYHRDLNLKNIVVRRESDGMKGYIIDFDRAKLFLGEVPL
                     ALARRNLDRLLRSANKLDPKREYFAAKDWKSFVDSYHERNADKP"
     gene            complement(1012..2112)
                     /gene="waaF"
                     /locus_tag="VFN58_01595"
     CDS             complement(1012..2112)
                     /gene="waaF"
                     /locus_tag="VFN58_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET8916711.1"
                     /translation="MVPRIWEKVLVVQTSFLGDTVLTLPLLSEIKRRFPSSQLSLLCT
                     PLGQALLTGHPAVDETIVDDKRSADRGWLGLWRKAKLLRKKGFTMALCPHKSFRSGLL
                     LFLAKIPYRVGFAQSKSSFLFHVRVNRNTGRHDVERTLSILEGLGIRTEECERGLDLP
                     TAAETRQEVAQRLRSLGVDTSKLLVGLHPGSVWATKRWSAEGFARLIGMLKERYPCEI
                     LLFGGPEDAQVIAQVQNLCGGRAVSLVERIALPELPAALGLCKVLVTNDSGPMHIAVA
                     RAVPVVAIFCATTPALGFYPYSSKAIVLEKDLPCRPCSSHGGRRCPLGTEDCIRLIGP
                     EQVFQAVERLLDSAGQPELEVANRYLPQFVTV"
     gene            complement(2116..2283)
                     /locus_tag="VFN58_01600"
     CDS             complement(2116..2283)
                     /locus_tag="VFN58_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET8916712.1"
                     /translation="MSISKELLDILACPKCKGDIHLNEQGDGLICDGCRLMYPIKDDI
                     PVMLIDEAVRL"
     gene            complement(2348..3289)
                     /locus_tag="VFN58_01605"
     CDS             complement(2348..3289)
                     /locus_tag="VFN58_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET8916713.1"
                     /translation="MRSIITGVAGFIGSHLAEKLLELGHEVVGVDKFLDNYARDFKDS
                     NLSQFVNHPSFKFINDDLVNIDLRQLLIQTDYIFHLAAQPGVRSSWGEEFSHYSHNNI
                     LATQVLLEACKQVKVRKFVYASSSSVYGDTDDLPMRESGGTRPVSPYGVSKLAAEHLC
                     YLYWKAVGMPTVSLRFFTVYGPRQRPDMFFHILMRRLLVGDEIPLYDDGEQSRDFTYC
                     KDIVDGLTAAAFYPGSGDVFNLGGGGEITLLKAIEMVEKISGRKAKLKRFDRQKGDVR
                     RTRASLDLASKKLGYRPSILLEEGLTRQWEWISALGK"
     gene            complement(3398..4462)
                     /gene="lpxK"
                     /locus_tag="VFN58_01610"
     CDS             complement(3398..4462)
                     /gene="lpxK"
                     /locus_tag="VFN58_01610"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET8916714.1"
                     /translation="MNGRWIRRVWERRGVLGRLVWVSFLPVSCLYWLGVQTRNILYSV
                     GWKTTQTLNEFVLSIGNLTVGGTGKTPTVVWVAQELQKRGFRIAVLSRGYNRKGSQPR
                     VLTANAPGDVDAFGDEPAMMARLFGLTVAVSNPRYKGALEAMKNNAVDVFILDDGFQH
                     RQLARDLDLVLLGSDSTGWVLPSGPFREPSKAVSRADFLLVTDSQDKWQRLVSQAGQR
                     LVFHGALKPVALIGFESRRWKEYPLSLLARRKIVAVAAIANPEPFYRIIHDWEGEIVE
                     VLEFKDHHDYSSKDWQRINRAARNADLIITTEKDILKLIRFPFGRERLLALRVVMAVE
                     NGDRLIQAVIDGIQKKSESN"
     gene            complement(4494..5810)
                     /locus_tag="VFN58_01615"
     CDS             complement(4494..5810)
                     /locus_tag="VFN58_01615"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HET8916715.1"
                     /translation="MICAKKQVTYWLYNALATVLLLTGLLFLPVLYLLGKRYSMGLGE
                     RLGFYGREVGNAVKGSRPVWIHAASVGEILSAGHLVEAIKKRFPSQKILVSAFTHTGY
                     LTARRVISDSSVIFFPLDHPWIVKRALFTFEPSLIVFLETEIWPNMLRLAHRRGTPTL
                     LLSGRFSKRSVKKYSSLSWFFRAVLENFTSMGMQSDEDADRAKKLGADPAKVLVTGNL
                     KLAGWGNNFPPNPMISEPFHLKKKAEGRCLLVAGSSHRGEEEILLDAFRSVKQRFPNF
                     QMVLAPRHPQRFPEVERLIKASGLEFEKKSQTNGRSSFMQDICLLDTLGDLKEFYALG
                     DIAFVGGSLVDAGGHNLLEPARVRRPVLFGPYMANFAAMAAEMKEKGGGVEVRGVQDL
                     VREITDLLNHADKRLTMGENAYQVAADEQRVGERTSELLSRYLQAR"
     gene            complement(5807..>6298)
                     /locus_tag="VFN58_01620"
     CDS             complement(5807..>6298)
                     /locus_tag="VFN58_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8916716.1"
                     /translation="DDTVRNNIAYGDPERRIDDVIAAAKAAHADEFIRSMPNGYDSQI
                     GEMGMQLSGGQRQRLAIARALLKDAPILILDEATSALDSDSERMVQEALEHLMTERTT
                     LVIAHRLSTIRNADRIVVLVDGTIAEEGTHEELLARKSEYSRLYSLQMLEEERSLNPE
                     TVH"
BASE COUNT         1571 a         1822 c         1462 g         1443 t
ORIGIN      
        1 ctgtccaagc gagcgtcgcc ttcggaaagc cgctatgaat gagattggcc agcggcaatg
       61 cccgggtcac atcaccgatg gatccatgaa gaaccacgag aatctttttt ggttcaaggc
      121 ttgtcagcat tgcgttcatg atatgaatcg acgaagctct tccaatcttt ggcagcgaaa
      181 tactcccgtt tcgggtccaa cttgttcgcc gagcgaagaa gccggtcgag gtttctccgc
      241 gccagggcca gcggaacctc tcccaagaac agtttagccc ggtcaaagtc gatgatatat
      301 cccttcattc cgtcggattc gcgacgcacg acgatattct tcaggttcag atctcgatga
      361 tagacgccct cccggtgcaa accgcgaagg ctggccgcca ctgcattaaa aaccttctca
      421 gctcctgtct gaccaacaaa accatcccgg atggctgtcc atagatcctg tccacccttt
      481 aactgtcgtg tcacaagcca gcctcggtag aacggccccc aaatacgcct aacgcaagcg
      541 gcaaaaacct cggctgtagg aatgccgcgt cggcggacct cttcggtgat caccaactct
      601 ctaaaaggcc gcggcggcca agtaaaaaaa atcccgttta acaaatgacg aaacaaacct
      661 ccgtgtcgat aaggccggat cagcgccgca tcgccattgc ggagctcaag gactcttaac
      721 tttcctctcc cttgaaatag gccggcctca tggtcatagg agtcgctcga ctttgtgcat
      781 tcctcgatag tgataaactt ttcaacatcc tctcgaacga tcattaggcc ctcgcggcca
      841 tcgatcactc ttttaaagcc tggcggaaca acgccccacg cccaatctcg ggaagtcatc
      901 atttttcaat atcacgattt atgtccgccg gcgcatcgcc actacaggcg aacgcgtttc
      961 cttttatgct tgtgcaggtt gaagttgaca acttttcagg cacagggcgg atcagaccgt
     1021 tacgaactga ggcaaatagc gattcgccac ctcgagctcc ggttggcccg ccgagtccag
     1081 gagtcgctcc accgcctgaa aaacctgttc ggggccaatg agccgtatgc aatcttcagt
     1141 gccgaggggg caacgccgcc cgccatggga gctacatgga cggcatggta gatctttttc
     1201 caacacaatc gctttggatg agtaaggata gaaaccgagc gcgggggttg ttgcgcaaaa
     1261 gatagcgacc accggaacag ctcgcgccac ggcgatatgc atgggtccac tatcattagt
     1321 gactagaacc ttgcaaagac ctaaagccgc gggaagctcg ggaagcgcaa ttctctcaac
     1381 caggctcact gcgcgaccgc cgcagaggtt ctggacttgg gcaataacct gagcgtcctc
     1441 gggaccgccg aatagaagta tttcacaagg atatctttct tttaacatcc ctatgagtcg
     1501 tgcgaaaccc tccgctgacc accgtttcgt agcccaaacg gatcctggat gcaatccgac
     1561 cagaagcttc gacgtatcga cacccagcga gcgcaaccgt tgagcgactt cttgccgggt
     1621 ctccgcggcg gtcggcaaat cgagcccccg ttcgcactct tctgtccgta tgcccaatcc
     1681 ttcgagaatt gaaagggtgc gctccacatc gtgtcgcccg gtattccggt tgactcgaac
     1741 atggaacagg aaagaactct tgctctgagc aaagcccacc cggtacggta tttttgccaa
     1801 aaacaagagc aaaccactcc gaaaagattt atgaggacat agggccatcg taaatccctt
     1861 tttcctgaga agcttcgcct tgcgccacaa gccgagccac cctcggtcgg cgctcctttt
     1921 gtcatccacg atggtctcat ccacggcagg atgacccgtc aaaagcgcct gccctagagg
     1981 tgtacaaaga aggcttagct gggagctagg gaatctccgc ttaatttcgg atagcaaggg
     2041 gagagtcaga acggtatcac cgagaaagct cgtctgaacc accaagacct tttcccaaat
     2101 gcgtggaacc atgatttaca gtcgcacggc ttcgtcgatc aacatcaccg gaatatcgtc
     2161 cttgatggga tacatcagac ggcagccatc gcagatcaaa ccatcaccct gttcgtttag
     2221 atgaatatcc cctttacatt tggggcacgc caggatatcc aacaactctt tgctgatcga
     2281 catcgagcgc tccttttgtc aaaactccca gattaaccgt aaaatgcaag tgtcatttac
     2341 atacttctca ttttcccagt gcagaaatcc actcccattg ccgcgtcaaa ccctcttcca
     2401 ataaaattga cggcctatag ccaagcttct tgcttgccag gtccaagctc gcacgggtac
     2461 ggcggacatc gcctttttgc cgatcaaatc tcttgagctt tgccttccgg ccgcttatct
     2521 tctcgaccat ttcgatagcc ttgagcagtg tgatctcgcc gccgccgccc aagttgaaca
     2581 catccccaga acccgggtag aaggcggctg cggtcaatcc gtcaacgata tccttgcaat
     2641 acgtaaaatc acggctttgt tctccgtcgt catataatgg aatttcgtcg ccaaccaaca
     2701 ggcgccgcat cagaatatga aaaaacatat ccggtcgctg tcgcgggccg taaacggtaa
     2761 aaaaacggag cgaaactgtc ggcattccaa ccgctttcca atagagataa cagagatgtt
     2821 ccgcggccag tttgctaacc ccataaggag ataccggtcg cgttccgcca ctttctctca
     2881 tcggcagatc gtcggtatca ccgtagacag aagaagacga ggcataaacg aattttctga
     2941 ccttgacctg cttgcacgct tctaacaaaa cctgcgtcgc taatatattg ttatgcgagt
     3001 agtgagaaaa ctcctccccc caactggagc gaacccccgg ctgggcagct aaatgaaaga
     3061 tataatccgt ttgaatgagt agctggcgca agtcaatgtt gacgaggtca tcgttgatga
     3121 acttgaagga cggatgattg acgaattgcg acaaattcga atctttgaag tcgcgcgcat
     3181 aattgtccaa gaacttgtcc acacccacga cttcgtgccc taactccaag agcttctcgg
     3241 ccagatgaga gccgataaag ccggcaaccc cagttattat tgatctcatt taaaggcctt
     3301 tcatgactgg cgctattctc cggctgactc cagaatcccg tcggtttgat ctcatagaag
     3361 gcagtccgca aacactccga accgggtatc caacacttta attgctttcg gatttctttt
     3421 gaatgccatc gataactgcc tgaatcagtc tatctccgtt ttcgacagcc atcaccactc
     3481 gcagggccaa caacctttcc cgcccgaatg gaaagcggat cagcttgagg atgtccttct
     3541 cggtcgtgat gatcagatcc gcgttgcggg ctgcccggtt gattcgttgc caatctttcg
     3601 aggagtagtc atgatgatct ttgaattcga gcacctcaac gatctctcct tcccagtcat
     3661 gaatgattcg ataaaagggc tccggattcg caatcgccgc gacggcaacg attttccggc
     3721 gcgccaagag actcaaggga tactccttcc aacgccgtga ctcaaacccg atcaaagcga
     3781 ctggcttaag ggcgccatgg aaaaccagcc gttgtccggc ttgggaaact aaacgctgcc
     3841 atttgtcctg tgaatcggta accagcaaga agtcggcccg actgaccgct ttgctgggtt
     3901 cgcggaatgg accggacgga agaacccaac ccgttgaatc ggagcccagg agtacaagat
     3961 cgaggtctcg cgccagttgc cggtgttgaa agccgtcgtc cagaataaat acgtccacag
     4021 cattattctt catcgcctcg agagcgccct tgtaacgcgg gttcgagacc gcaacggtca
     4081 atccgaaaag gcgggccatc attgccggtt cgtccccgaa agcatcgacg tcccccggag
     4141 cgttagccgt cagcacccga ggctgactgc cttttctgtt ataaccccga ctgagcacag
     4201 caattctaaa accgcgcttt tgtaactcct gggccaccca aacaaccgtg ggtgttttcc
     4261 ccgtgccgcc cactgtcaag ttgccgatgc tgaggacaaa ttcattgaga gtttgggttg
     4321 ttttccagcc aacggaatag agaatgttac gagtttgcac acccaaccaa taaagacagg
     4381 atacgggtaa gaacgagacc cacacaagtc ttcccaaaac cccacgacgt tcccaaaccc
     4441 gacgaatcca ccgaccattc atctaactcg gatcaccgct ttaatttgca gcttcatcgc
     4501 gcttgaagat aacgggacaa taattcagaa gtacgctcgc cgacacgctg ttcatccgcg
     4561 gcaacttgat aagcgttctc acccattgtc agacgtttgt ccgcgtggtt cagaagatcg
     4621 gtgatctcgc gaacaaggtc ctgtacgcct cgaacttcaa cccctcctcc cttttctttc
     4681 atctccgcgg ccatagccgc aaaattggcc atgtagggac caaataaaac aggtctccgg
     4741 acacgggcgg gctccaaaag attgtgcccc cctgcgtcga ccagactccc acccacaaac
     4801 gcaatatctc cgagcgcata aaattctttg agatccccca aggtatccaa aaggcaaatg
     4861 tcctgcataa aagaacttcg tccattggtt tggctcttct tttcaaactc caatccgctc
     4921 gccttgatta accgttccac ctctggaaag cgttgcggat gtctgggcgc caataccatt
     4981 tgaaaattgg ggaaacgttg cttcacggat cgaaaagcat ccaaaaggat ttcctcttcg
     5041 ccacggtgtg aactccccgc taccaacaaa cacctcccct cggctttctt cttaagatga
     5101 aagggctccg agatcatggg attaggaggg aaattatttc cccaccccgc gagcttcaag
     5161 ttgccggtca cgagcacctt tgccggatcc gctccaagct ttttggcgcg gtcggcgtct
     5221 tcgtcactct gcatacccat agaggtaaaa ttttccagta cagctcgaaa aaaccaggat
     5281 aacgaagaat atttcttaac cgatcgttta gaaaaacgac cactcaagag caatgtaggc
     5341 gtccctctcc ggtgcgccaa gcgaagcatg ttgggccaaa tttctgtctc taaaaacact
     5401 atcagtgacg gctcaaaggt gaaaagcgct ctcttgacga tccatggatg atccagggga
     5461 aagaagatta ccgaactgtc cgatattacg cggcgcgccg tcagataacc cgtgtgggta
     5521 aatgccgaaa ctagaatctt ttgggatgga aaacgttttt tgattgcctc cacaagatga
     5581 cccgcagaca atatctctcc caccgacgcg gcgtggatcc acaccggccg agaaccttta
     5641 acggcgttcc ccacctcgcg accataaaaa ccaagccgct cgcccaatcc catgctgtat
     5701 cgcttcccca acaggtacag caccggcaaa aaaagcagac cggtcagcaa taatacggtc
     5761 gctagcgcgt tgtaaagcca gtaagtcacc tgttttttgg cacagatcaa tgaacggtct
     5821 ccgggttgag tgacctttct tcttccaaca tctgcaatga atagagtcgg ctgtattcgc
     5881 tcttccgtgc taacaactct tcgtgggttc cttcttcggc gatggttccg tcaaccaaaa
     5941 ccacgatacg atccgcgttc cttattgtcg ataaacgatg ggcgataacc agagttgtcc
     6001 gttcagtcat caaatgctct agagcttcct gaaccattcg ttctgagtcc gaatcgagcg
     6061 ccgaagtggc ttcgtcaaga attagaatcg gtgcatcttt gaggagtgcc cgcgcaatcg
     6121 ccagcctctg cctttgcccg ccagacagct gcattcccat ctcgccgatt tgagaatcgt
     6181 atccatttgg catggacctt ataaactcgt ctgcatgagc tgccttggct gcggcaatga
     6241 catcgtctat ccgtctttcc ggatcgccat aggcaatatt gtttcgcacc gtatcgtc
//
LOCUS       DASSWZ010000068         4881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_935956, whole genome shotgun sequence.
ACCESSION   DASSWZ010000068 DASSWZ010000000
VERSION     DASSWZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935956"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VFN58_01625"
     CDS             complement(<1..908)
                     /locus_tag="VFN58_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8916717.1"
                     /translation="MIRMLEGKVALVAGATRGAGRGIAVELGAAGATVYLTGRTTRAQ
                     RSEYNRPETIEETADLVRQAGGQGIAVQVDHLDPAQVQPLVARIEREQGRLDVLVNDV
                     WGADFFIEWNVPVWKHSLERGLRMLRLAIDTHIITSHFALPLLIKNPGGLVVEMTDGT
                     AEYNNAKYRVSLFYDLAKTSVIRMAWAQAHELRSHQCTAVALTPGWLRSEMMLDAYGV
                     SEANWRDAIAKQPHFVITETPRYVGRAVAHLAGDPEVERWSGQSLSSGQLAKVYGFTD
                     LDGSQPDAWRYVVEVQDAGKPADATG"
     gene            complement(1036..1596)
                     /locus_tag="VFN58_01630"
     CDS             complement(1036..1596)
                     /locus_tag="VFN58_01630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8916718.1"
                     /translation="MSEQEHKDQLTVRLARETDLDDVEEMVNDFVKGHPAEKHPRSRS
                     VLCAAYFGDAPVARLVVATRNGRVIGMGQWTLIYDMFWGMYGANAEWLYVRPEYRGRG
                     VVVAIVAEICAQVRQAGGEFLRGGGDDEVERLYERVAIGRPTHECHVSAEAFQVLADL
                     AGLAPREIVRRLPSPELNRVAARARI"
     gene            1975..2511
                     /locus_tag="VFN58_01635"
     CDS             1975..2511
                     /locus_tag="VFN58_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET8916719.1"
                     /translation="MGDKIIKSYISPKIEIRKSELGGKGLFARDRINKGDIVIDYAGG
                     PGQFISMKKADALYEDGNDYMIQVDNDRFFVATNSKELEEADFINHSCNPNCGIKGHL
                     QIVAMRDIESGEEITFDYAMTESSDYSMDCKCGAKNCRKTITGNDWKLPILQKRYNGY
                     FSNYIQIKLMNLIVILGK"
     gene            complement(2857..3327)
                     /locus_tag="VFN58_01640"
     CDS             complement(2857..3327)
                     /locus_tag="VFN58_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8916720.1"
                     /translation="MNEQYAVSITRFPLFQGFTLDGARMLLESGEIKEYSPGEVLLKE
                     GDSPTFVLLVLTGKMQVFVERQQRDLVLTDAGPGTILGELAVLCGIPRSASVRASEKS
                     AALQWSAAAFRSLLLRDAFFSQRIFRESLRTLIEKERSLIDSLIRSQGGSNPSE"
     gene            complement(3506..3928)
                     /locus_tag="VFN58_01645"
     CDS             complement(3506..3928)
                     /locus_tag="VFN58_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8916721.1"
                     /translation="MSRFQRAVPVLQVRDVERSMGWYVDTFGFQPDPFPEKPPYGFAI
                     LRRDDSEIMLQSADVEEKFHSGDGWAVYLRTSGEDLLDLAKAIGRVTPLIRGPERMFY
                     GLVEFEVVDPDGYHVCVSGPLPASVNVPRAREHEDATR"
     gene            complement(4015..>4881)
                     /locus_tag="VFN58_01650"
     CDS             complement(4015..>4881)
                     /locus_tag="VFN58_01650"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916722.1"
                     /translation="WIAQDLKLFEKYGLESELVYVSGARPIQAMLGGSIDASQVGGAA
                     TVSAAAQGADVVILGTIFSRLNFAVHASPQIKQISDLKGKTLVTGTIGGNTYFAALLF
                     LSRFNWVPNRDVNLIGVGGSPEVLGALTQGRFPAGVLTAPTTHIATRMGFREIFDLAS
                     LDFPFPTLSVVSTRKYIEANPDVILSVLRATSEAIHLYKTRPEVTLPVIAKYMRVPKD
                     DPALLQAQETFAKHLNLTLTPSLDGIKFILDFLAEQRPALKGKNPADFVNVSFLKKLE
                     DEGFFKKLSSSH"
BASE COUNT         1153 a         1378 c         1277 g         1073 t
ORIGIN      
        1 tatccggtcg cgtcggctgg tttaccggca tcctggacct caacaacgta gcgccaagca
       61 tcgggctgtg aaccatcaag atctgtgaag ccatacacct ttgcgagctg tccgctcgac
      121 agcgactgac cactccagcg ctcaacttcc ggatctccag caaggtgcgc gaccgcacgg
      181 cctacgtagc gtggggtttc ggtgatgacg aagtgcggtt gcttcgcgat ggcgtcacgc
      241 caatttgctt cactgacgcc gtaggcatct agcatcatct cggaacgtag ccagccggga
      301 gtcagggcaa cggcggtgca ctgatgggac cgaagctcgt gtgcctgcgc ccaagccatg
      361 cgaatgactg aggtcttggc gagatcatag aacagggaca cccggtactt tgcattgttg
      421 tactctgcag tgccgtccgt catctcgaca actagtccgc ccggattctt gatcaaaagg
      481 ggcagtgcga agtggctggt gatgatatgc gtgtcgatgg caagccgcaa catccgcagc
      541 cccctttcga gagaatgctt ccacacgggc acattccact cgatgaagaa gtccgccccc
      601 cacacatcat tgaccaggac atccaggcga ccttgctcac gctcgatacg cgccactagc
      661 ggttgcactt gcgccggatc caaatgatcc acttgcacgg cgatgccctg gccaccagcc
      721 tgccgcacca gatcggccgt ctcttcaatc gtttcaggac gattatactc ggaacgctgc
      781 gcgcgcgtgg tgcgaccagt gaggtacacg gtggctccag ccgcgcccag ttcgacagcg
      841 atgccacggc ccgccccgcg cgttgcccct gcgaccaacg ccactttgcc ttcaagcatt
      901 cgaatcatga ttcagctctc ttgcggtcgt ttacgcataa gactgatggt cagcggcgcg
      961 gcaagtggcg catgccttgc gtaggcaagg tgtgtgacac cctgatgcat gcgctcattg
     1021 tgcgatcgtg cagacctata ttcgcgcacg cgcagccact cgattcaact ctgggctcgg
     1081 aagccggcga acaatctccc gcggagccaa accagccaag tccgcaagca cttgaaacgc
     1141 ctctgcagaa acgtgacatt cgtgcgtcgg ccgtccgata gcgacacgtt catataggcg
     1201 ctctacctcg tcgtccccac ctccgcgtag aaactcaccc ccagcttggc gtacttgggc
     1261 acagatttcc gccacaatcg caacaacgac tccacgtccc cgatattccg gccgcacgta
     1321 gagccactcc gcgttggcac catacatgcc ccaaaacatg tcgtagatca gcgtccattg
     1381 tcccataccg atgacgcgcc cattccgggt tgccacaact agccgcgcca cgggagcgtc
     1441 gccgaagtac gccgcgcaca acaccgaccg tgaccgcgga tgcttctccg ccggatgacc
     1501 tttgacgaag tcgttgacca tttcttcgac gtcgtccaag tcagtttcac gagcgagtcg
     1561 gaccgttagt tggtccttgt gttcctgctc cgacatgctt gttaacgcgc tccggtcaac
     1621 tgccaatgtt gcacaacgcc gggttcgagg gcgtctccgc tctcagtcgt accgccacat
     1681 gcctagcgta ctgctcatat ccaagtcgcc gggcaatact gatggacgcc atgttggttt
     1741 caagagtctg atagaggagc agcttgcctt cctggagcgc gcgcccgaca acggcgctgg
     1801 ttgtggccgc tgcccaaccg cgaccgcggt gggcgcgatg cgtgagaatg caagggccac
     1861 ggtctctgcc ttgctccgtc tgtcaactac taattatata gaaatactta acgacgatat
     1921 actcttacag tatggggaaa atcaaatgta gacattctag aatataagag atgcatggga
     1981 gacaaaatca taaaatctta tatatcgcca aaaattgaaa tcagaaaaag tgaacttggc
     2041 ggaaaaggat tatttgctag agacagaatc aataagggtg atattgtaat tgattacgcc
     2101 ggtggtccgg gacaattcat aagcatgaaa aaagccgacg cattatatga ggacggcaat
     2161 gattatatga ttcaagtgga caatgacaga ttctttgtgg ctacgaatag caaagaactg
     2221 gaagaggctg attttataaa tcattcctgt aatcccaact gcggtataaa agggcaccta
     2281 cagattgtag cgatgagaga tatagaatca ggcgaggaaa ttacatttga ttacgcaatg
     2341 acggaatcat ctgattactc aatggattgt aaatgcggag ctaaaaattg ccgcaagaca
     2401 attaccggca atgattggaa attgccgatt ttacagaaac gatataacgg atatttttcc
     2461 aattatatac agataaagct gatgaattta atagttattt tgggaaagta gtcggcacaa
     2521 ctaagcttgg tgataataac caagtattaa ccgaggcgga cctggcaatc ggggaaaaga
     2581 attatctctt tgtttgaaat ggaacgcgct cgaatcccgc ggcctctcta tcggtgtcca
     2641 tttagacctc ctcaactgag tcagctgaaa accctatgaa tgcttcgaca aggctcagcg
     2701 tgaacggaaa aatctccggg taatttccac tgctcgtccg ttcgtccctc gctaaactca
     2761 gggtttttcg gcagaattct aaactcatat ttcaactgga aaaacgatgc tgtcccagca
     2821 agaccaacat acatctctcc gcctccatcc gtggccctac tcgcttgggt tgctgccgcc
     2881 ttgcgagcgg ataagtgaat caatcagcga gcgctctttc tcgataaggg tgcgaagcga
     2941 ttctcggaat atccgctgcg agaagaaggc atcgcggagg agtaggctgc gaaaagccgc
     3001 ggcgctccat tgcagggccg ccgatttctc gctcgcgcgc accgaagccg atcgcggaat
     3061 gccgcatagc acagccaatt cacccaagat cgtacccggc ccggcgtcgg tcagcaccag
     3121 gtcacgttgc tggcgttcga cgaagacttg cattttaccg gtgaggacga gcaggacgaa
     3181 tgtggggcta tctccttcct tgagcagcac ttctcctgga gagtactcct tgatttcgcc
     3241 agactccaac agcattcgag caccatccaa agtgaacccc tggaatagcg gaaaccgcgt
     3301 gatcgagaca gcgtattgct cattcacgga aagcacctcc gttggttttg cgtttcgcct
     3361 ccacgatagg gtaacagaaa cgctgcggct ccctcagtcg tcgcagctga gggagcggct
     3421 tgaagcgcca agttaagctt tgaaaaatgg tcgctttccc tgtgttctcc tgcctgctca
     3481 ggccaaaaac gtcttcgtgc gtcggttagc gcgttgcgtc ttcgtgctca cgtgcacgtg
     3541 ggacgttaac actcgccggc aagggcccgc ttacacaaac gtggtagcca tctgggtcga
     3601 caacttcaaa ctcgacgagg ccatagaaca tgcgctccgg ccctcgaata agtggggtaa
     3661 cccgcccgat ggcttttgcg aggtccaaga gatcctcacc ggatgtgcgc agatatacgg
     3721 cccaaccgtc gccgctgtga aacttctcct ccacgtcagc ggactgcagc atgatttcag
     3781 agtcgtcacg cctgaggatc gcgaatccgt agggcggttt ctcaggaaac ggatcgggtt
     3841 ggaaaccgaa cgtatcaacg taccaaccca tactccgctc gacatctcta acttgcagca
     3901 caggtaccgc ccgctgaaat ctcgacatgt gatcctccat ttacttccag ctcattagtt
     3961 agcgctttag ctgagccgag cgttgccaga atatcccttg tccaacggac gttactagtg
     4021 gctgctcgat agttttttaa agaacccttc gtcttctagc tttttcagaa agctcacgtt
     4081 gacgaaatcc gcgggatttt ttcctttaag cgcagggcgc tgctcggcga gaaaatccag
     4141 gatgaacttg atgccgtcga gcgatggcgt cagggtcaag tttaagtgct tggcgaaagt
     4201 ttcctgagcc tggagcagag cgggatcgtc tttgggcaca cgcatatact tggcgatcac
     4261 cggcagtgtg acttccggcc gtgtcttgta caaatggatc gcctctgaag tcgctcgcag
     4321 tacgctgaga atgacgtcgg ggttcgcttc gatatatttc cgggtgctga caacggacag
     4381 agtgggaaaa ggaaaatcca ggctggcgag gtcgaaaatc tctcgaaagc ccattctcgt
     4441 cgctatgtgc gtggtcggcg ccgtcagcac acccgccggg aacctgccct gggtcagcgc
     4501 gccaagaact tccggcgagc cgccgacacc gatcagattc acgtcccggt taggaaccca
     4561 attgaatctc gagaggaaga gcagcgccgc aaagtaagta ttgccgccga tcgtaccggt
     4621 aaccagggtc tttcctttga gatcgctgat ctgcttgatc tgcgggctcg catgtaccgc
     4681 aaaattaagc cgggaaaaga tagtgcccaa gatcactaca tcggctccct gggctgctgc
     4741 cgagacggtg gccgctccgc cgacctgaga cgcgtcgatg gatccgccca gcatagcttg
     4801 aatcggcctg gcgcccgaca catagaccag ctccgactcg agaccatatt tttcgaaaag
     4861 cttcaggtcc tgcgcaatcc a
//
LOCUS       DASSWZ010000069         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1134162, whole genome shotgun sequence.
ACCESSION   DASSWZ010000069 DASSWZ010000000
VERSION     DASSWZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134162"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="amrB"
                     /locus_tag="VFN58_01655"
     CDS             complement(<1..665)
                     /gene="amrB"
                     /locus_tag="VFN58_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HET8916723.1"
                     /translation="MDRIRPAAVAGSFYPSEIEELGRLIDECFESSPLGPQGYRSPSS
                     SLIGGLVPHAGYVYSGPCAAHFYALLDNSIQRVILLGVNHQARGHRAALSPADFWQTP
                     LGIVRIDQESNHLLQDAVHFLKQDEVAHAREHSIEVQLPFLQRVLGEFSLIPISLAHI
                     SMEECVELGTAVADLLRNNRQSGTRTVMMASSDLSHYLSPKETAELDGIALEQVLALN
                     PRA"
     gene            complement(683..1627)
                     /locus_tag="VFN58_01660"
     CDS             complement(683..1627)
                     /locus_tag="VFN58_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8916724.1"
                     /translation="MRFITFGRKNQQRIGLIGPQDQIIDVAEITRRYLRGGNPPYLAS
                     MQAFIEAGGKALQAARKAEKYAAGKTPEEQKKLARAGGLLKSSQAKLLAPIPWPRKNV
                     VMLGINYREHVDEGARARSLDIKYPEWPVYFTKPATSVIGHMGKVVHHKVTDKLDWEI
                     ELAVVMGKKGRDIPKAKAYDYVFGYTVCLDMTARELQRQHGQWFKGKSLDTFCPLGPW
                     IVHKSALPDPQQVRLICRINGEVMQDGNTRDMIFDIPTIIESLSSGLTLEPGDIISTG
                     TPSGVGFARVPPVYLKPGDKVEGEVEGIGILQVEIVEP"
     gene            1928..2125
                     /locus_tag="VFN58_01665"
     CDS             1928..2125
                     /locus_tag="VFN58_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916725.1"
                     /translation="MREGDRILINKGISKKVFIVARVDVPHSNERVFVSKSGGKYTIG
                     LKVWKTDYAWSEKNQMWVPKH"
     gene            2238..3608
                     /gene="holA"
                     /locus_tag="VFN58_01670"
     CDS             2238..3608
                     /gene="holA"
                     /locus_tag="VFN58_01670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET8916726.1"
                     /translation="MKRDLENVVADIKNSRGPQLLLLFGDDLQVQGACKTLLDNLVPE
                     NQRGFNLERFDGRSAPWDQIEASLNTPPFFPGKKVVWVENVTYFISREQKGELGEKVR
                     QAWSEGKKDEAVRLLLDLLAVEGWTQEQLEQAQSASSSEPLNELLEVDDAEARADTQA
                     LLVYSRSQEMDLSRRRSSEEQRLAEMLERGLPPWDFFLMTAVQVDRRTRLYRRLEEMG
                     AVLQLVIERDRSGRISRESLAVFVSQRLRQAGKTIEPQAREMILLRAGDELRGLQQEL
                     EKLFLYVGDQPTIRSQDVDAIFADQGDGWVFDLTRCIAERNAVAALGHLARLLAQGEH
                     PLKLLGTIAAEVRRLLAARQLMDGEVRGRWKRGLTYQQFQQNVLNQGAPILTRNPYAD
                     FMCFQRADQFSLDELRACLTSSYEADLRLKSRGNNPRLIMEKLILNMCLGSRKESGLV
                     GRRIGI"
     gene            3680..4147
                     /locus_tag="VFN58_01675"
     CDS             3680..4147
                     /locus_tag="VFN58_01675"
                     /inference="COORDINATES: protein motif:HMM:NF019509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET8916727.1"
                     /translation="MAVQTCFSPQGRCSAHIVREIEKAQKELLIAVYAFTNDELARAV
                     AQAKKRGVSVQIVLDREFDAANEKSQGRFLDEQKIPLKRISGIKPAAPEKEPGLMHQK
                     FAVIDRKIVFTGSYNWTRSADSANDENLLWFRDAGPLAEEYRKVFFQLWERKG"
BASE COUNT         1002 a         1110 c         1229 g          995 t
ORIGIN      
        1 aatgcgcggg ggttcagcgc caatacttgt tcgagagcga tcccgtccaa ttcagcggtc
       61 tctttgggtg aaagatagtg gctgagatcg ctgcttgcca tcatgacggt tctggttccc
      121 gactgcctgt tgttccgcaa aagatctgcg acagccgtgc cgagttcgac acattcttcc
      181 atagagatgt gggcgagcga tatgggaatc agggagaatt cgccgagcac gcgctgcaaa
      241 aaaggtagct ggacctcgat gctgtgctcg cgggcatggg cgacttcatc ctgtttcaga
      301 aaatggactg cgtcttgcag cagatggttt gactcttgat cgatccttac tatgcccaac
      361 ggcgtctgcc aaaagtccgc tgggctgagc gcagcccgat ggcccctagc ctgatggttg
      421 acacccaata agatgacgcg ctgaatggaa ttgtctaaaa gcgcatagaa atgcgccgcg
      481 caagggccag aatagacgta gcccgcgtgg gggaccaatc caccgatcaa ggatgatgag
      541 ggcgacctat agccttgggg ccccaacggg ctcgactcga aacattcatc gatgagtcgg
      601 ccaagttctt caatctctga aggataaaat gaaccggcta ccgcagcggg cctgattcgg
      661 tccataaccc cgaaagtttg cgttagggct cgacgatctc aacttgaagt ataccaatgc
      721 cttcaacttc accttcaact ttgtcaccgg gctttaggta caccggcggc acgcgagcga
      781 aacccacgcc ggaaggagtt ccggtgctga tgatgtcgcc cggctccaat gtcagtccac
      841 tcgacaagct ttcgatgatc gtcgggatat caaagatcat gtcgcgggtg ttgccgtcct
      901 gcatgacctc accattgatc cgacagatca accgcacctg ctggggatcc ggcagagcgc
      961 ttttgtggac gatccagggt cccaacggac aaaaagtatc cagcgatttt cccttgaacc
     1021 actgtccgtg ttgacgctgg agctcgcgcg cggtcatatc gaggcacacc gtgtaaccga
     1081 acacgtagtc gtaggctttg gcttttggaa tgtcgcggcc cttctttccc ataaccacgg
     1141 ctaattcgat ttcccagtcg agtttgtcgg tgaccttgtg gtggacaacc ttgcccatgt
     1201 gaccgatcac cgacgtcgca ggtttggtga aatacaccgg ccattcaggg tacttgatgt
     1261 ccagcgaacg tgcccttgct ccttcatcga catgttcgcg gtaattgatg ccgagcatca
     1321 ccacgttttt tctcggccag ggaatgggag cgagcagctt tgcttggctc gatttcagca
     1381 gcccgccggc gcgagcgagt ttcttttgct cctcgggagt tttccctgcc gcatatttct
     1441 ccgccttcct tgcagcttgc agtgctttac cacctgcctc aataaaggct tgcatactcg
     1501 ccaaataagg cggattgcca cctctcaagt agcgccgggt tatctccgcc acatcgatga
     1561 tctgatcctg ggggcctatg agaccgattc gttgctgatt cttccggccg aaagtaataa
     1621 accgcattct ctcctccgag ttggaaaata ctctaacgaa agatccacgc cggtccctaa
     1681 cagcctgcaa gcgttgggtc tgaccgtcga cgggaactta ttcagcgtag ataccaacga
     1741 ccctagtttt gggaaatgac cgttgtcctg cgacagctat aggagagggc tagttgattg
     1801 tcaagctgga caccccagta aggcatcgaa gccggtttcc gatactctgc gtgcgtttgc
     1861 ctgccaaaat gagcaatggt aacttttcct catgaaaggc aagggcaaag gagagaaccg
     1921 gacctcaatg cgcgagggag accgcatcct gatcaacaaa gggatttcaa aaaaagtctt
     1981 tatcgttgcg cgggtcgatg tgcctcattc caacgagaga gttttcgttt ctaagtccgg
     2041 cggaaagtac accatcggtc ttaaagtctg gaaaacggac tatgcctgga gtgaaaaaaa
     2101 tcagatgtgg gtgccgaaac actagcaggc cttacaggtt tcgcaggcgc cgcctttgag
     2161 atctttttga acagcacggg aaaacggttc tttccagcag aattaatagc tcacaatctg
     2221 agaatttgcc gcctaacatg aagcgggatt tggaaaacgt tgtcgctgat atcaagaata
     2281 gcaggggtcc acagttgctg cttttgtttg gcgacgatct gcaagtgcaa ggggcttgca
     2341 aaaccctcct tgacaatctg gttccggaaa atcagcgggg atttaacctc gaacgcttcg
     2401 acggtcgatc ggcgccgtgg gaccaaatcg aagcttcatt gaatacgccg cccttttttc
     2461 cggggaaaaa ggtggtgtgg gttgagaacg taacttattt tatttcgcgc gagcaaaagg
     2521 gcgagttggg agagaaggtt cgtcaggctt ggagcgaagg caagaaagac gaagcggtta
     2581 ggttgctgtt ggatttgcta gctgtggagg gttggacaca ggagcaattg gagcaggcac
     2641 aatccgcctc ttcttcggaa ccgctgaacg agcttctgga agtcgatgac gccgaggcgc
     2701 gcgcagatac ccaagccctt ttggtgtatt ctcgtagtca ggaaatggac ctaagccggc
     2761 gtcgaagttc cgaggaacag agacttgcag aaatgctcga gcgcggattg ccgccgtggg
     2821 actttttctt gatgacggcg gtgcaggttg atcgtcggac acgtctttat agacggctcg
     2881 aagagatggg cgcggttttg cagctcgtca tcgagcgcga tcgtagcgga aggatcagtc
     2941 gggaaagtct ggcggtgttc gtcagccagc ggctgcgaca agccggaaag acaatcgaac
     3001 cccaagccag agaaatgatc ttactgcgcg ccggggatga gttgcggggc ttgcagcaag
     3061 aactggaaaa gctgtttctt tatgtgggcg atcaaccgac aattcggtcc caggatgtgg
     3121 atgcgatttt tgcggatcaa ggcgacggtt gggttttcga tttgacacga tgcattgcag
     3181 agcgaaatgc cgttgcggcg cttggccatt tggcccgtct gctggcgcag ggagaacatc
     3241 cgctgaagct cctaggtacc atcgcagcgg aggtacgaag gctactagca gcacgccagt
     3301 taatggacgg ggaagtacgc gggcggtgga agcgaggttt gacctatcag cagttccagc
     3361 agaatgttct caatcaaggt gcgccaatcc tgacgcgcaa cccctatgcg gatttcatgt
     3421 gcttccagcg agcggatcag ttttctctgg atgagctgcg cgcttgtttg accagcagct
     3481 atgaggcaga tctccgtttg aagtccaggg gtaacaaccc ccggctcatc atggaaaagc
     3541 taattctcaa catgtgcctg ggctcacgaa aagaaagcgg gcttgtcggc cgccggattg
     3601 ggatatgaag agcagggggc gcgaacttgc gatctgttgc tgtctcctat ccttgtgtgc
     3661 tgtcacctct tgggccgaga tggcggtgca aacctgtttt agcccgcaag ggagatgttc
     3721 ggcgcacatt gttcgtgaga ttgagaaggc gcaaaaagaa ctgctgatcg cggtgtatgc
     3781 attcaccaac gacgagctcg cgcgcgcggt ggcgcaagcg aaaaaacgcg gcgtttcggt
     3841 ccaaatcgtg ttggaccgag aattcgatgc agctaacgaa aaatcccagg gtagattttt
     3901 ggatgagcaa aaaattcctt taaagcgcat ttccggcatc aagccggcgg cgccagagaa
     3961 agagcccgga ctaatgcacc aaaagttcgc cgtgatcgat cgaaagatcg ttttcacggg
     4021 ttcttacaac tggacccgtt ccgcagatag cgccaacgac gagaacctgc tttggtttcg
     4081 cgacgccgga cccctggcag aagagtatcg caaggtcttt tttcagctct gggagagaaa
     4141 agggtgaagc aattctcgcc ggccataacg ctcggtctct tgatcgcatt tctagcgggt
     4201 tttgtctcgc ctttgctcta tcaacggtgg aatccctcct ttcccgcaac cggcgagaat
     4261 ccgacgaatg agaaaaattc tgagttccgc cttgacgttg agccaccgag ctttcgcgtg
     4321 accttcctgc ccacgc
//
LOCUS       DASSWZ010000070         8327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_737866, whole genome shotgun sequence.
ACCESSION   DASSWZ010000070 DASSWZ010000000
VERSION     DASSWZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737866"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /gene="pnp"
                     /locus_tag="VFN58_01680"
     CDS             complement(<1..1902)
                     /gene="pnp"
                     /locus_tag="VFN58_01680"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET8916728.1"
                     /translation="MIKKVELDFHGRPLSIEVGKVAKQADGAALVRYGETVVLVTAVA
                     AKELKTDTDFFPLTVDYQEKTFAAGKIPGGFFKREGRPSEKEILTCRLIDRSIRPLFS
                     EGLRCETQVIATVLSADRENDPDMVAMLGASVALQVSDIPFSGPLVGVRIGRIDGRWV
                     INPTQSQLLESDMDIFLSGSRDAIVMVEGGAKMVPEDEILEALFTGHEAIQPLLQIQE
                     EIRRDIGKPKRQVPLAELDAAIVRQVEQLALSKLQQAIEIPEKLERYKQIGEIKGEVV
                     AKMLAEFPQKEKDIKGAFDELKRNCFRGLIIQRERRIDGRGLKDIRPITCEVEILPRT
                     HGSALFTRGETQALVVTTLGTASDEQKIDALIGEHYKKFMLHYNFPPFSVGEVKFLRG
                     PSRRDIGHGNLAERALLPVLPPEESFPYTVRIVSEVLESNGSTSMATVCGGSLSLMDA
                     GVPVLAPVAGIAMGLIKEGEHVRVLSDILGDEDHLGDMDFKVAGTPNGVTSLQMDIKI
                     SGVNRDIMRQALYQAREGRLHILGIMTETMGSHRASVSGHAPRITTLKVRPDKIREII
                     GPGGKVIRGIIEATGVKMDVEDDGTVTIASSDEAASRKAIEMVQRIAAEAEVGKIYKG
                     TVRKIVDFGA"
     gene            complement(1970..2239)
                     /gene="rpsO"
                     /locus_tag="VFN58_01685"
     CDS             complement(1970..2239)
                     /gene="rpsO"
                     /locus_tag="VFN58_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET8916729.1"
                     /translation="MGIAQPNRQVVIEQFRVHQTDTGSPEVQVALLSQRIDHLTGHFK
                     IHVKDHHSRRGLLKLVGQRRRLLDYLKKSDFERYQSLIQRLGIRK"
     gene            2457..3062
                     /locus_tag="VFN58_01690"
     CDS             2457..3062
                     /locus_tag="VFN58_01690"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HET8916730.1"
                     /translation="MARTVFGAAFLSALLNSSASLIAAEYYRWVDQNGVVHFTDNLHN
                     IPETQRGSAGRIQSIEPPRVAPPPVPIPPSKASIPFEKQGQVVVVEATLNKKTAAKFV
                     VDTGASYTMISSAVAKELDIDTEQNRRTAPFQTANGIIQAPLVSLESITVGGMEIRNL
                     TAAVHDAVSDARIAGLLGLNFLSNFRMDIDTQKGVLHLEKK"
     gene            complement(3078..5843)
                     /locus_tag="VFN58_01695"
     CDS             complement(3078..5843)
                     /locus_tag="VFN58_01695"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET8916731.1"
                     /translation="MSQVTELNVPGGDPRTQKGLKIGRYFTRAGVDPYDEIEWEQRAA
                     SITNEKGKVVFEQNNVEIPKAWSLMATNVVVSKYFRGPLGSPQREQSVRQMIGRVVDT
                     ITSWGRKDGYFASDEDAQSFHDELTHILVHQKACFNSPVWFNCGIEAKPQCSACFILA
                     VDDTMESILDWYRKEGIIFKGGSGSGVNLSKIRSAREQLAGGGTASGPVSFMKAADAS
                     AGVIKSGGKTRRAAKMVVLNIDHPDIEEFIKCKVEEEKKAWALIEAGYDSSLDGPAYG
                     SVFFQNANNSVRVSDGFMQAVQEDREWKTRYVKSGEVCETFKARDLLRMIADSTYFCG
                     DPGMQYDTTINDWHTCANTARINASNPCSEYMHLDNSACNLASLNLLKYLRDDGVFDT
                     EAFRHAVDIISLAQDMLVDNSSYPTAEITANAHAFRELGLGYANLGALLMSLGLPYDS
                     DAGRAYAAAVTALMSGEGYLQSARNAAATGAFAGFEVNRQPMLRVLNKHRSHAYKISP
                     SHVPLDLLTAAREAWDEACDEAEKFGVKNSQISVLAPTGTIAFMMDCDTTGVEPDIAL
                     IKYKKLVGGGLLKIVNNTVPRALKRLGYDVKQIQEIVEYIDEHETIEGAPHIKDEDLA
                     VFDCAFKPANGSRSIHYMGHVKMMGAVQPFISGAISKTINMPTDVTVEEIMHAYVESW
                     KLGLKAVAIYRDGSKRTQPLNTSKDKEEKKAAVGEGKESRLLRRKLPDERRSITHKFD
                     IAGHEGYITAGMYEDGQPGEIFITMSKEGSTISGLMDSFATAISMALQYGVPLRVLVD
                     KFSHMRFEPSGFTKNPDIPMAKSIMDYIFRWLATKFLDGEAQQEVGVVKPEPEEPGNR
                     ENLAIKKVIPIASQAPRGGAPVANISSVTSLYQQDAPPCPDCGAIMIRSGACYKCMNC
                     GAVSGCS"
     gene            6080..6553
                     /locus_tag="VFN58_01700"
     CDS             6080..6553
                     /locus_tag="VFN58_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET8916732.1"
                     /translation="MARHDPEIERLGQVLDKSLKRMEISTRLDEYGVWPIWNDIVGTT
                     IARNAQPEKIRNGTLFVKVTSPVWMQQLQYMKEMIAEKLNQRLKTDVVKNIFFVVGRI
                     HIETPDVDSMPTATSSAAGQDSRPDEDFLESIRDPEIRQAFKRLLKGYSRRHRKS"
     gene            complement(6550..7389)
                     /locus_tag="VFN58_01705"
     CDS             complement(6550..7389)
                     /locus_tag="VFN58_01705"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET8916733.1"
                     /translation="MSFAPLLGCGENRNAMSMIPDEHVSVLAGAADLKREGETLDVLF
                     EGKLKLFQAHSGYRFSLDALLLAHFMTCRRGDMVADLGTGNGVIALTLAYLHSSLSIV
                     GVEIQLSMVDRASRNVRMNGFQERITISQADVRNIQETFGPESFAAVVCNPPYRRTTS
                     GRISPNVERKIARHEIAAGLADFLRAGAYLLPIKGRIAMIYPAVRVVDLIQSMRNVNL
                     EPKRLRMVHSFADAKASLVLVEAVKGGRSGNEALSPLIVYRKGKQYTTEVEAMLAGRA
                     TLY"
     gene            7497..8267
                     /locus_tag="VFN58_01710"
     CDS             7497..8267
                     /locus_tag="VFN58_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HET8916734.1"
                     /translation="MLSLLARIGRVTLEQLAGMGRMFLFLFSAFAWTFRPPARLRLIV
                     YHVRSIGVDSLSVVLLSGVFTGMVMGLQGYYSLRKFNAESFLGSAVALGLLRELGPVL
                     SAFMVTGRTGSAMAAEIGSMKVTEQIDALYSMAINPIQYLVSPRIIAGLISMPLLTAI
                     FDVVGIYGAYLVGVGLLGVSSGSYFAGMEGSVVFHDVWSGLLKSVSFGLIITWVCCYK
                     GFNAPPMATGVGQATTESVVLCFVLILVWDYFMTSVML"
BASE COUNT         1795 a         2375 c         2114 g         2043 t
ORIGIN      
        1 agcgccgaaa tcgacaatct ttctcaccgt ccctttgtag atcttcccga cctcggcctc
       61 ggcggcgatc ctttgcacca tctcaatggc ttttctcgaa gccgcttcat cgctggacgc
      121 aatggtcacg gttccgtcgt cttccacgtc catttttaca cccgtggctt caatgatgcc
      181 gcgaataacc ttaccgccgg gtccgatgat ttcacgaatt ttatcgggcc tgaccttgag
      241 ggtggtaatt cgcggcgcat ggcccgagac actggcccgg tgcgacccca tggtctcggt
      301 catgatgccg aggatatgca accggccttc ccgcgcctgg tacaacgcct gccgcatgat
      361 gtcgcggttc acaccgctga tcttgatgtc catctgcagc gaagtgacgc cgttcggcgt
      421 gccggcaact ttgaagtcca tgtcgccgag gtgatcctca tcacctagaa tatcggagag
      481 cactcgcaca tgttcccctt ctttgatcag acccatggcg atccccgcca ccggcgccaa
      541 taccggcacg ccggcatcca tcagggaaag cgatccgcca cagaccgtgg ccatcgacgt
      601 agagccattg gactccaaaa cttcagatac aatgcgcact gtgtaaggaa aactctcctc
      661 gggcggcaat accggcagca acgcgcgttc ggcgagattg ccatggccga tgtcgcggcg
      721 gctcggccca cgaagaaact tgacctcacc gacgctgaac ggaggaaagt tataatgcag
      781 catgaacttt ttgtaatgtt cgccgattaa tgcgtcaatc ttctgttcgt ccgatgccgt
      841 cccgagtgtc gtcacaacca gcgcctgggt ctccccgcgg gtaaacaggg ccgatccgtg
      901 agtccgcggc agaatttcga cctcgcaggt aatcgggcgg atatctttta atccgcgtcc
      961 gtcgatgcgg cgctctcgct gaatgatcag gccacggaag caattccgct tgagctcgtc
     1021 gaatgcccct ttaatgtctt tttccttttg gggaaattcc gccagcatct ttgcgacgac
     1081 ttcgcctttg atttcgccga tttgtttata gcgctccaat ttctctggaa tctcgatggc
     1141 ctgttggagc ttcgacagtg ccaactgttc gacttgccga acaatcgctg cgtccagttc
     1201 tgccaacggc acttgacgct tgggcttacc gatgtcccgc cgaatctctt cctgaatctg
     1261 caaaagcggt tggatggctt catggccggt aaataaggcc tcgagaattt cgtcttccgg
     1321 aaccatcttg gcgccgccct cgaccatgac gatggcatcg cggctgcccg acagaaaaat
     1381 atccatgtcg ctttccagga gttggctttg ggtgggattg atgacccatc ggccgtcgat
     1441 tcggcctatc cgaactccta caagcggtcc gctaaatgga atatccgaaa cctggagcgc
     1501 cactgaggct cccaacatcg cgaccatatc gggatcgttc tcacggtcag ccgataaaac
     1561 cgtcgcgatc acttgggttt cacagcgaag cccttccgaa aagagcggcc gaatggagcg
     1621 atcaatgagt cgacaggtaa ggatctcctt ttccgacggg cggccctcac gcttgaaaaa
     1681 gccgccggga atcttccctg cggcaaaagt tttctcctga taatcaaccg ttaacgggaa
     1741 gaaatcagta tcggtcttaa gctctttggc ggccactgct gtcaccaaaa ccacggtttc
     1801 accgtagcgg accaatgcgg cgccgtctgc ctgtttcgcg actttgccga cttcgatcga
     1861 caaaggacgg ccgtgaaaat ccaattccac tttcttaatc atattaggtt cctctgagag
     1921 gagacgcgct taacggaggc ggtacaggag ggtaactggt caggaatggc tacttcctta
     1981 ttcccagccg ttggatcaga ctctgatatc tttcgaaatc gcttttcttc agataatcca
     2041 gaagcctcct cctctgtcct accagcttca ggaggccccg acgggaatgg tgatccttga
     2101 cgtgaatctt gaagtgcccg gtgaggtggt cgatgcgctg actcaataag gccacttgca
     2161 cttcaggaga gccagtatcc gtctgatgaa ccctgaactg ctcgataact acttgcctat
     2221 tcggttgagc aattcccatg tgatctccgc tctttctctc tctttctttg ttttagttgg
     2281 acaaatttct accataccga cgctgtcttg taaaggtgcc tggaaaaagc aattgacgcc
     2341 agcagcggcc aaacatcggc ggacatgcac tcggagcttc cttatctatc ttatgcgagc
     2401 caccgttggt gagttagttt tcgtcggtag cgagttatga taagattctt tctgtcatgg
     2461 cacgcacagt tttcggcgcc gccttcttat ccgctctctt gaatagctca gcctcactta
     2521 tcgcagctga atattatcgc tgggtggatc aaaacggtgt agtccatttt accgacaatt
     2581 tgcataacat cccggaaaca cagcggggta gcgcggggcg tatacagtcc attgagcccc
     2641 cgcgggtcgc gccgcctccc gttccaatcc cgccgagcaa ggcctcgatc cctttcgaga
     2701 agcaagggca ggtcgtcgtc gtcgaggcca cgcttaataa gaaaaccgca gcgaagtttg
     2761 tcgtcgatac cggcgcgagc tacacgatga tctctagcgc cgtagccaaa gaactcgaca
     2821 tcgacaccga acaaaatcgc cggaccgcac cctttcaaac ggccaacgga ataatccaag
     2881 cccccctagt gagtctcgag tcgattaccg tcggtggcat ggaaatccgc aatctgaccg
     2941 ctgccgtgca tgatgccgtt tcagatgcac gaatcgctgg cttgctgggc ctcaacttcc
     3001 tgagcaattt ccgtatggac atagataccc agaaaggcgt gctccactta gagaagaagt
     3061 agtcgagcgg ggcagtctta cgagcacccg ctcaccgcgc cgcaattcat acacttatag
     3121 caggcgccgc tccgaatcat gatggcaccg cagtcggggc agggcggcgc gtcctgctgg
     3181 taaaggctcg tcacgctgct aatgttggcc accggcgcgc cgccgcgcgg cgcttgcgat
     3241 gcgatcggga tcaccttctt aatggcgaga ttttccctat tgccgggttc ctctggctct
     3301 ggcttcacga caccgacctc ctgctgcgcc tcaccgtcga ggaacttggt cgccaaccag
     3361 cggaaaatat agtccataat cgacttcgcc atcgggatat cgggattctt cgtgaaaccg
     3421 gacggctcga accgcatatg gctgaacttg tccactaaaa ctcgcagcgg caccccgtat
     3481 tgaagtgcca tcgagatcgc ggtcgcgaag gaatccatga ggccggaaat agtagaaccc
     3541 tccttagaca tggtgatgaa aatctcacca ggctggccgt cttcgtacat gccggcagta
     3601 atgtagcctt catgcccggc gatatcgaac ttatgggtaa tcgaccttct ttcgtcaggt
     3661 agcttgcggc gtagcagccg actttccttc ccttctccca cggctgcttt cttctcctct
     3721 ttgtctttag aggtgtttag gggctgcgtc cgttttgaac cgtcacggta aatcgccacc
     3781 gctttaaggc ctagcttcca ggactccaca tacgcgtgca tgatctcctc cactgtaaca
     3841 tcagtgggca tattgatggt tttggagatc gcaccggaaa tgaacggctg caccgcgccc
     3901 atcatcttga catgccccat gtagtggatc gaccggctgc cgttggccgg cttgaaggcg
     3961 cagtcaaaga ctgccaggtc ttcgtccttg atatgcggcg cgccttcgat ggtttcatgc
     4021 tcgtcgatgt actcgacgat ctcttggatc tgcttgacgt catatccaag ccttttgagc
     4081 gcccgcggca cggtattgtt gacgatcttc aacaggccgc cgccgactag tttcttatat
     4141 ttgatgagag cgatatccgg ctcgacgccc gtggtgtcgc agtccatcat aaaagcgatg
     4201 gtcccggtcg gagccagcac ggagatctgc gaatttttta cgccgaattt ttccgcctca
     4261 tcgcaagcct catcccatgc ctctcgggcc gccgtcaaaa gatccagcgg cacatgcgat
     4321 ggcgagatct tgtaagcatg ggagcggtgt ttgttgagca cccgcagcat gggctggcga
     4381 ttgacctcga aaccggcgaa ggccccggtg gccgccgcat tccgggccga ttgcagatag
     4441 ccttccccgg acatcaaagc cgtgactgct gctgcatagg cgcggcctgc atccgagtca
     4501 tagggaagac ccaaagacat gagtaaagcg ccgaggttgg catagccaag ccccaattcg
     4561 cgaaacgcgt gcgcattggc ggtaatttcc gcggtcgggt aagaggaatt atcgaccagc
     4621 atgtcctgcg ccaggctgat gatatccacc gcgtggcgga aagcttccgt gtcaaacacg
     4681 ccatcgtccc ggaggtactt gagcagattc agcgatgcca agttgcaggc cgaattgtcc
     4741 agatgcatat attccgagca cgggtttgaa gcattgatac gagctgtgtt ggcgcaagta
     4801 tgccaatcgt tgatcgtggt atcgtattgc attccaggat cgccgcagaa gtaagtgctg
     4861 tccgcgatca tccgaagcag atctcgcgcc ttaaaggttt cgcatacctc accgctcttc
     4921 acataacgag tcttccactc ccggtcttcc tggaccgcct gcatgaaccc atccgatacc
     4981 cgcacggagt tattggcatt ctgaaaaaag acgctgccgt aagccggccc atccaagctg
     5041 gagtcatatc cggcttcgat gagcgcccaa gccttcttct cttcttcgac tttacacttg
     5101 atgaattctt cgatgtccgg gtgatcaatg ttgagcacca ccattttagc agcgcgccgt
     5161 gttttgcctc cggatttaat cacgccggct gaggcgtcgg ctgccttcat aaaggacacc
     5221 ggacccgagg cagtaccgcc gcccgccaat tgctctctag ctgaacgaat cttcgacaga
     5281 ttgacgccgg aaccggagcc gcctttgaag ataatgcctt ctttgcggta ccaatccagg
     5341 atcgattcca tagtgtcatc caccgcgagg atgaaacaag cggaacactg gggttttgct
     5401 tcgatgccgc aattgaacca cactggactg ttgaagcagg ccttctggtg caccaggatg
     5461 tgggtgagtt cgtcatggaa gctttgcgca tcttcgtcgc tagcaaaata gccgtccttc
     5521 cggccccaac ttgtgatcgt atcgaccacg cgtccgatca tctggcgcac gctttgttct
     5581 ctctgcggac ttcccagcgg cccacggaaa tacttcgaaa ccaccacgtt ggtggccatt
     5641 aaagaccaag cttttggaat ctctacgtta ttttgttcga agacgacttt gcccttttca
     5701 ttggtaatgg aagcggcacg ctgctcccac tcgatctcgt cgtatggatc tacgccggcg
     5761 cgggtaaaat accgccctat tttgagccct ttttgggtcc tggggtcccc tccaggaaca
     5821 ttaagttcag tcacttgtga catcgtggtt gccatcgcgc ttgctccctt cacccttgga
     5881 ttgtcaatat atggtgtgaa tcacgctgga aatatactag attttgtatt ggtaaagctt
     5941 ttgaaaaatt ttgtcaagaa aaattttctt tcccagcatt tatttttaac attgcctgcc
     6001 tttccgctaa acccatcgcc agataatatt ctgtagagtc tcgacatggc atctgctaca
     6061 ttcttttctg gttgagaaaa tggcgaggca cgacccagag attgaacggc ttggacaggt
     6121 gctcgataag tctctcaagc gtatggagat ttctacgcgg ttggatgagt atggtgtctg
     6181 gccaatctgg aacgacatcg ttggtacgac catagcccga aatgcccaac ccgaaaaaat
     6241 tcgcaacggc actctattcg tcaaagtaac gagcccggtt tggatgcagc aacttcagta
     6301 tatgaaggaa atgattgctg agaaactcaa tcaacggctc aagacagatg tcgtcaaaaa
     6361 tatttttttc gtcgttggac gaatccatat tgagaccccc gacgttgact caatgccaac
     6421 agctacgtct tccgctgccg gacaggattc ccggcccgat gaggattttc tcgagtcaat
     6481 acgcgacccg gagatccgcc aagccttcaa gcggctgctc aaaggctact cacggcggca
     6541 ccgaaaaagc taatatagcg tcgcccttcc tgccagcatc gcctcaactt cggtcgtgta
     6601 ttgctttccc ttcctataaa caatgagcgg cgagagtgcc tcgtttccac ttcttccgcc
     6661 cttcacggct tccactagaa ccagagacgc cttggcatcg gcaaaggaat gaaccatccg
     6721 taagcgcttc ggttcaaggt taacgttgcg catagattga atcaaatcga cgacccgaac
     6781 ggcgggataa atcatcgcga ttcttccttt gatcggcaac aaataagccc ctgctcgcaa
     6841 aaaatcggca agccctgcgg cgatctcgtg gcgggcgatt ttcctttcga cgttagggct
     6901 tatgcgaccg ctggtcgttc gtcgataggg cgggttacaa accacggcag caaaactttc
     6961 ggggccaaaa gtttcctgga tattgcggac atcggcttga ctgatcgtta ttctctcttg
     7021 aaacccgttc atccggacat ttctgctggc gcgatcaacc atacttagct gaatttcgac
     7081 acccacaatc gacagcgacg aatggaggta ggctagggtc aaagcaatca ccccgttacc
     7141 cgtaccgaga tcagcgacca tgtcgccgcg ccggcaagtc atgaaatggg ccagcaacag
     7201 cgcgtcgaga gaaaaacgat agccgctgtg agcttgaaaa agcttgagct tgccttcaaa
     7261 cagcacgtcc aatgtttcac cctccctttt taggtcggcc gctccagcta aaactgacac
     7321 gtgctcatcc ggaatcatcg acatcgcgtt tctgttttcc ccgcaaccga gcagaggagc
     7381 aaaactcatg gctccactca tcgtttctat ttggttaatt tatctcagtt gcttgaaaat
     7441 ctctgaactt ttctataata gcctgatttt agtatcacgg gacattgcaa caaaaaatgc
     7501 tttctctact cgctcggatt ggacgcgtca ccttggagca actcgctggc atgggtcgaa
     7561 tgttcctgtt tctcttttct gccttcgcct ggacctttcg acctcccgca cgcctgcgtt
     7621 tgattgtgta tcacgttaga agcattggag ttgactcgct atcggtggtg cttctttccg
     7681 gcgtatttac cggcatggtc atggggcttc agggctatta tagccttaga aaattcaacg
     7741 ccgaaagctt ccttggctcg gctgttgccc tgggtttgct gcgcgagctc ggtccggtgc
     7801 tttcagcttt tatggtgacc ggcagaaccg ggtcagcgat ggcggcagag atcggcagta
     7861 tgaaggtcac tgaacagatc gatgcgctct actccatggc gatcaatccg atccagtatc
     7921 tggtctcgcc gcgcatcatc gccggcctca tctcgatgcc gctgctcacc gctatctttg
     7981 acgtggtcgg gatctacggc gcttaccttg tcggcgtcgg actcctgggt gtcagctccg
     8041 gcagctattt tgcgggaatg gaaggcagcg tggtcttcca tgatgtctgg agcggccttc
     8101 tcaagtcggt gagtttcggt ctgattatca cgtgggtttg ctgctacaag ggatttaacg
     8161 cgccgccgat ggctaccggt gtcggccagg cgaccaccga atcggtcgtc ttgtgcttcg
     8221 tcctgatcct ggtttgggac tacttcatga cttcggtcat gctctaacag gctgctgaaa
     8281 aggactcata tgcttcgttg cgctccatcg cctcgctcca acgtact
//
LOCUS       DASSWZ010000071         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1020718, whole genome shotgun sequence.
ACCESSION   DASSWZ010000071 DASSWZ010000000
VERSION     DASSWZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020718"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..944
                     /gene="trpA"
                     /locus_tag="VFN58_01715"
     CDS             132..944
                     /gene="trpA"
                     /locus_tag="VFN58_01715"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET8916735.1"
                     /translation="MSCIERKFADLQRRGEAALIPFVTAGDPDLATTLKILRALEKGG
                     ADCIELGIPFSDPTADGPTIQRSSERALRNGLSLPRIFRLVRQFRRTSEVPIVFFGYF
                     NPIFHYGLENFCRAAARAGADGILCVDLPPEESAELKRWTDAEGLDLIFLLSPISGPD
                     RVQLVAGRGRGFIYYVSVTGVTGARQALDNRLRAQVARVRRATSLPVGVGFGISTPKQ
                     AAWIAGFADAAVVGSALVERIEKAKGSAEKARCAGAFVSELKRAMKQVAKKS"
     gene            1009..2319
                     /locus_tag="VFN58_01720"
     CDS             1009..2319
                     /locus_tag="VFN58_01720"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET8916736.1"
                     /translation="MDAYSETLNTIYNLRGGVIDLRLDRMNQALALFEHPEKQFPSFH
                     IAGTNGKGSTAAMLHRILSRAGYQVALYTSPHLVSFTERIRVGDEEIAPNEVVALAQE
                     IWDRTGAANVSLTFFEFVTVMAFVHFARRKIDVAVVEVGLGGRLDATNLVRSVVSLIS
                     TISKDHEAYLGSDLLSIAREKGGIIKQGIPVICGALPTDVAALLENIANEHTAPSYFL
                     SRDFTFSLKNNGRFDYRGLKWRLIDLVVALRGRHQKRNAALALAGLEIAGRDFPVPEA
                     ALREGLKTVRWPGRFEIVQDHPAIVLDGAHNGEGVRALVEELENFREQRRVKLLFASM
                     EDKDWRLMLESLSAVVDEIVLTRVNMGRCADPFYLASQLGGKVAHRAIGNARSALDYV
                     LDGADSNDVILIAGSLYLIGEIRPIVQEIAAAKSGKKNDPHSPV"
     gene            2297..>4384
                     /gene="lptD"
                     /locus_tag="VFN58_01725"
     CDS             2297..>4384
                     /gene="lptD"
                     /locus_tag="VFN58_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HET8916737.1"
                     /translation="MILILQSRKAMRFGKSFGIRALLAVVAISCVAPAWGQATPGAKS
                     AAEKIDVTADSLSFGDGGTQIEAKGNVEIKREVMTLKADEVRINRETNEMEAKGKVSL
                     DDPNWKVKSAESMQFNLQKETGELQKGDMFIEEGHVSVSGQRLQKFGGQTYHIDQGFF
                     TTCMCESGPAHWRISADEVDLTLEGTGIIRHGYFYVYDIPVLYIPYGVIPLQTERQTG
                     FLFPEVGQSGQNGFRYLQPFYWAISKSTDATLSFNVETKTRLGVLGEFRTKLNQESDF
                     QFQPSFFNELFRKNADADIVDKTIADPHIPLHRWGVVGTHRYTTETDWLTYSDFGAYS
                     DPLFTRELVDRFDLPATRERTIQASRFGRSQFGVFRSWGDTYLNGDWRFYQDFIQPAK
                     TTLQRTPEVSFWGRRFLSGFPLEFRWRAESVSYLRRQDCPGSGGTTGYCGDGLRLDLR
                     PEVVLPFRLASYLFGSVSVTPRETAYYLYSPVNPGDQGVSRELVEIRGNIGTTLSRVF
                     SWNGPGGGAIRHVFEPELSYFFVPGVNQDKIPIMDDIDRVRRRNVVMFAVGNRIWRRS
                     GGASPSPSPDQEGESLNPFISNVQELGSLRLALGYNVAAARKGADSLTDLDMNLRLTP
                     TNFLNFAFDGGVSPGSWEITQGRATIAFTDPRPITRRALDPDFLRPNSFSIGYRYLVN
                     GPNGFLASNSNINL"
BASE COUNT          982 a         1118 c         1235 g         1049 t
ORIGIN      
        1 cggaagggga gataaggata tgatgacagt tggggattat ctcggggtga ctctctaaat
       61 gtggttcaag tttaagattt ttctttactt tgagcccgga accttgaatt tttgaagctc
      121 ctgtccgaac catgagctgt atcgaaagga aatttgccga cctacaaagg agaggagagg
      181 ccgcgctgat cccctttgtt accgcgggag atcccgatct tgcgaccacg cttaagatcc
      241 ttcgggcttt agaaaagggc ggtgcagact gtatcgagct cggaatcccc ttctctgacc
      301 ccacggcgga cgggccgact attcaacgat cgtcggaacg cgccttaaga aacgggcttt
      361 cattgccgcg aatttttcgg ctggtccgcc agtttcgccg tacgtcagaa gtgccgatcg
      421 tgttttttgg ttacttcaat ccgattttcc actacggctt ggaaaacttt tgtcgtgcgg
      481 cggctcgtgc cggcgctgat gggatactct gcgtcgatct gcccccagag gagagtgccg
      541 agctcaagcg ctggaccgat gcggaaggac tggatctcat ttttctctta tcccctatca
      601 gcggtccgga tcgtgtgcag cttgtggctg gaaggggtcg aggatttatc tattacgttt
      661 ccgtgaccgg tgtcaccgga gcgcgccaag cgctcgataa ccgactacgt gcgcaagtcg
      721 cacgggtgcg ccgagccaca tcgctgcccg tcggagtcgg ctttggaatc tcgactccaa
      781 agcaggcagc ctggatcgcc ggctttgccg atgccgccgt ggtcggcagc gcattggtcg
      841 aaagaatcga aaaggcaaag ggaagtgcgg agaaagcccg gtgcgccggc gccttcgtgt
      901 ccgagctcaa gcgggcaatg aagcaagtcg ctaaaaaatc ttagagcttg ggcagttcct
      961 cacaagtcgc gcccatcagc taggaagcgg tcccttcaac ttatccccat ggatgcgtat
     1021 tcggaaactc tcaacacgat ctacaacctg cgcggcggag tgatcgatct ccgcctggat
     1081 cgcatgaacc aggctttagc tctctttgag cacccagaaa aacaatttcc ctcgtttcat
     1141 atcgcgggga ccaacggcaa aggatctacc gccgccatgt tgcatcgcat cctgtctcgg
     1201 gccggttatc aggttgctct ttacacctca ccccatctcg tatcttttac ggaacgaata
     1261 cgtgtaggag atgaagagat tgctccaaac gaagtcgtag ccttggcgca ggagatctgg
     1321 gaccggacag gagccgctaa tgtttcgctg accttctttg aattcgtcac ggtcatggcc
     1381 tttgttcact ttgcccggcg aaagatcgat gtcgcggtgg ttgaagtcgg cttaggcggt
     1441 cgattggatg ctacgaatct ggtgcggtct gtggtgtcat tgatttcgac tatttccaaa
     1501 gaccacgagg cttatctcgg atccgacctg ctttctatcg cccgggaaaa agggggaatc
     1561 ataaagcaag gaattcctgt gatttgtggc gcattaccga cagacgtcgc agccctgcta
     1621 gagaatattg ccaatgagca cacggctcct tcttactttc tcagccgtga ttttactttt
     1681 tccttgaaaa ataatggtcg tttcgattat agaggtctaa aatggcgttt aattgacctc
     1741 gtcgtcgcac tccgcggtag gcatcaaaag cggaatgcgg cgcttgcgct agccggtctt
     1801 gagattgccg ggcgcgattt cccggtgccg gaggcggccc tgcgggaggg attaaaaaca
     1861 gtccgctggc ctggacggtt tgaaatcgtg caggatcatc ccgcgattgt tctggacggt
     1921 gcgcacaacg gggagggtgt aagagcgctg gttgaggagc ttgaaaattt tcgcgagcag
     1981 cgcagggtca aactgttgtt cgcttccatg gaagataagg attggcgtct catgttggag
     2041 agcctttccg cggtggtgga tgagatcgtc ttgacgcggg tcaatatggg acgctgtgca
     2101 gatccttttt acttggcctc tcaactgggc ggaaaagttg cgcatcgagc gataggaaat
     2161 gcgcgatcag cgctcgacta cgttctcgat ggcgcggact ccaatgacgt tattcttatt
     2221 gccgggtccc tttatcttat aggtgagatt cgaccgatag tccaagaaat cgcggcggca
     2281 aagtccggca aaaaaaatga tcctcattct ccagtctaga aaagcgatgc gcttcggaaa
     2341 aagtttcggc atccgagcgc tattggccgt cgttgcgatt tcttgcgttg ctcctgcgtg
     2401 gggacaagcc actcccggcg ccaaatcggc cgccgagaag attgacgtaa ctgccgacag
     2461 cctgtctttc ggcgatggcg gaacgcagat tgaagctaaa gggaatgtcg agatcaagcg
     2521 cgaggtgatg acactcaaag ccgatgaggt tcggatcaac cgtgaaacga acgagatgga
     2581 agcaaaaggg aaagtctcgc tggacgaccc taattggaag gtgaaatctg cggagtccat
     2641 gcagttcaat ttacaaaagg aaaccggtga gcttcagaaa ggcgacatgt tcatcgaaga
     2701 gggccacgtc agtgtctcgg ggcaaaggtt gcagaaattt ggcgggcaaa cctatcatat
     2761 tgaccaggga ttcttcacga cctgcatgtg tgaatccggc ccggcgcatt ggcgaatctc
     2821 ggccgacgaa gtcgacctta cgcttgaggg cacaggaatc ataagacatg gctacttcta
     2881 tgtttatgat atcccggttt tgtacatacc ttacggtgtt atcccgctgc aaaccgaacg
     2941 acaaaccgga tttctattcc cggaagtcgg tcagtcgggc cagaacggat ttcgatacct
     3001 gcaaccgttt tactgggcga tatcgaagag cacggatgca accttgtcct ttaacgttga
     3061 gactaaaacc cgattgggcg tgcttggtga atttcgaacg aagcttaacc aggagtccga
     3121 ttttcaattc cagccatcct ttttcaacga gctgtttcgc aagaacgcag atgcggacat
     3181 tgtggacaag acgattgccg atccgcacat tccgctacat cgttggggcg tcgtcgggac
     3241 ccatcgctac acgacagaga ccgactggct cacctacagc gacttcggcg cctacagtga
     3301 ccctctgttt acgcgcgagt tggtggaccg cttcgatcta cccgcaacaa gggaacgaac
     3361 cattcaagca agccgttttg gccggtctca gttcggggtg tttagaagct ggggagacac
     3421 gtatctcaac ggggactgga ggttctacca ggatttcatc cagcccgcca agacaacgtt
     3481 acaaaggaca ccggaggtct ccttttgggg ccgccggttt cttagcggat ttccactgga
     3541 atttcgctgg cgagcggaaa gcgtgagtta tctccgcagg caggattgtc cggggtctgg
     3601 gggcacgacc gggtattgcg gcgacgggct tcgtctcgat cttagaccgg aagtggtgct
     3661 cccctttagg cttgcctcct atctgtttgg gtcggtgagc gtcacccccc gcgagacggc
     3721 ttattatcta tactcaccag tcaatcctgg ggatcaaggc gtttcccgtg agctggtgga
     3781 aattcgcggc aatatcggta ccaccctgag ccgcgtcttt tcttggaatg gaccaggggg
     3841 cggcgcaatc aggcatgtct ttgagccgga attgagctat ttcttcgttc ccggtgttaa
     3901 ccaggataaa attcctatca tggacgacat cgaccgcgta cgacgcagaa atgttgtgat
     3961 gtttgccgtg ggcaacagga tatggagaag gtcgggtggc gcatcaccca gtccctcccc
     4021 tgatcaagag ggcgaatcgc tgaacccgtt catttctaat gtccaggagt taggatcgct
     4081 acggctggca cttggttaca atgttgcagc agcgcgaaaa ggcgctgaca gcctgacgga
     4141 tctcgatatg aacctacgac ttacccccac aaatttccta aattttgcct tcgacggcgg
     4201 ggtaagccca gggtcatggg agatcaccca aggtcgggca actatagcgt tcactgatcc
     4261 tcgtccgatt acacggcggg cattggatcc ggacttcttg agaccgaatt ctttcagcat
     4321 tggttaccgt tacttagtca atggaccgaa tggttttctg gcctcgaact caaatatcaa
     4381 cttg
//
LOCUS       DASSWZ010000072         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1360434, whole genome shotgun sequence.
ACCESSION   DASSWZ010000072 DASSWZ010000000
VERSION     DASSWZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360434"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFN58_01730"
     CDS             <1..921
                     /locus_tag="VFN58_01730"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916738.1"
                     /translation="CAATVFAEEITVSYATLTAAYMDHIVAMEKGYALEEGLNVKVMR
                     AGGGTATQTLLSGQLHFSSSAGTALSAALRGGLVKIVYTNMSRPTYRLVSNKPEIKTL
                     QDLVGKKIAINTFGDTGHLATLLLFKKYGLDPKSFLFIAVRNEARFPAFVSGSVDAAP
                     LSPRDIAQVGPLKGHILADTTNEVQLVWNGVAVSSKLLSENPLLVERFLRAVAKGREF
                     ARLYKEPTLAMIAKHTPSPLDVLSLDYDTVLGSMTEAGWVTDDVLRDEIMTRGELIKV
                     TKLPEAGQLFDYRIIKKVYTELKKSWKPKP"
     gene            968..1975
                     /locus_tag="VFN58_01735"
     CDS             968..1975
                     /locus_tag="VFN58_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916739.1"
                     /translation="MAKKIREFMTHRDPDEHIWVERDESAVIGTARMGQRMVPKIQPF
                     DLLRSAPRSANCTRAMVWEGDSLRIATEHIDGVEEQLTRAADHDVVYFQFCGRSKIES
                     ECGVVDLEPGEMVLMPAAISHRSTGKEECLRLRVATRELVNLGVDPEKPIVETQFKVR
                     PSEPFARDGNGRQESENGRTLEHITFWDPQSDVWIERDASALIGTVTSGSRPVKKIRG
                     FDYFTGMTGKGGAKAPVLYHGKEFRIDVYNLEEEQRGFHRGCDEDEIWFQFRGHSLNH
                     TEWGAMELDAGQTAMIPRGIAHRITGSPGFLRMVFYSRGLVQPKAFNNISERETRFEV
                     Q"
     gene            2001..3119
                     /locus_tag="VFN58_01740"
     CDS             2001..3119
                     /locus_tag="VFN58_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916740.1"
                     /translation="MKLSDWISLGLSVLATLCLPSFLPAADGQTEWERTLAAAKKEGP
                     PVIGIPASSELRKAIGTRFKEKFGIDIELFPSRGPENVTRIIKEYSAGVRYFDVLVAG
                     GATPLAMVAAGAADDFQPYMILPEVKEAKNWWGGHIWEDNVSTKKYIYAFLCYTSETF
                     WHNTSQVEPQEMRSFDDLFNPKWKGRIGFLDPRNPGSGQNTWAFLWKVKGEEFLGKLV
                     QQDLLITQNQRQIADALAKGKLAFTIGLSHYSYEPFIKAGLPVKPVANIKEGAHANNG
                     SGVVTVVKNPPHPNSTKVFINWLLGKEGQELYGKAMLQGTRRLDVDTQWLKESGIEAC
                     KDIMTVEDYYRRETHLESSVNKLRKPATALAEKLLSRN"
     gene            complement(3187..3498)
                     /locus_tag="VFN58_01745"
     CDS             complement(3187..3498)
                     /locus_tag="VFN58_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916741.1"
                     /translation="MSKYLMNKLIHHVNMSESAEDEYVSNPRGFVEKWEKSQKLKFTP
                     EEREALATKDYGKLYALGAHPFTLWSFTEAVWIHEKPREELVKDYKEKAARAGYPDFK
                     T"
     gene            complement(3512..>3781)
                     /locus_tag="VFN58_01750"
     CDS             complement(3512..>3781)
                     /locus_tag="VFN58_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET8916742.1"
                     /translation="DWKILSTVFTAVFIAELGDKTQLATMLFASDKDASKLTVFVGAA
                     LALIVTSAIGVAAGSAISQHVSEKTLHYLAGIGFIAIGIWTLIKA"
BASE COUNT          915 a          946 c         1073 g          847 t
ORIGIN      
        1 tgcgccgcga cggtctttgc tgaagaaatc accgtgtctt acgctaccct cacggcggcc
       61 tacatggatc atattgtggc aatggagaag ggctacgccc tcgaagaagg actgaatgtt
      121 aaagtcatga gagctggagg agggacggca acccaaacct tgctgtccgg acaattgcat
      181 ttcagctcgt cggcaggtac tgccctgagt gcggcgctac gaggcggtct cgtcaaaatt
      241 gtttacacca acatgtcgcg ccccacctac aggctcgttt caaataagcc tgagattaag
      301 accctgcaag atctcgtggg aaagaagatc gctatcaata ctttcggtga tacgggtcat
      361 ctcgccacgc ttttgctttt taaaaagtat gggctggatc cgaaatcttt tcttttcatc
      421 gcggtcagga acgaggctcg ctttcccgct tttgtgtcgg gctccgttga tgctgcgccc
      481 ttgagtccca gagatatcgc tcaagtgggc ccgctgaagg gtcacattct cgccgacacg
      541 accaacgaag ttcagttggt ttggaacggt gttgcggttt ccagcaaatt gctctcggaa
      601 aaccctctgt tggtcgagcg tttcctccgc gcagtggcca aaggaagaga atttgctcgc
      661 ctttataagg aaccgacgct tgcaatgata gccaagcata cgccatcgcc gttggacgtc
      721 ttgtccttgg actatgacac ggtcctggga agtatgaccg aggcaggctg ggtgacggat
      781 gatgtgctga gagacgaaat catgacgaga ggagaactga ttaaggtaac caaactgcct
      841 gaggccggac agctgtttga ctaccgcatt atcaagaaag tctataccga gctcaaaaag
      901 agctggaagc cgaagccttg atcgaaaacg acaaattaat gtcagctaaa actaaaggag
      961 tcaccgtatg gcaaaaaaaa tccgtgagtt catgacccac agggaccctg atgaacacat
     1021 ttgggtggaa cgggatgaga gcgcggttat cggcaccgcc cggatggggc agagaatggt
     1081 gccgaagatc cagccgttcg acttgctccg cagcgcaccg cgctccgcta actgcactcg
     1141 ggcgatggtc tgggaaggcg acagtttgcg tatagcgact gagcacatcg acggagtcga
     1201 ggagcagctc acccgggcgg cggatcatga tgtggtttat ttccaattct gcggccgttc
     1261 gaagatcgaa agtgaatgcg gggttgtgga cctggagcct ggcgaaatgg tcttgatgcc
     1321 tgcggccatc tcgcatcgat ccaccgggaa ggaagagtgc ttgcgccttc gcgtggctac
     1381 ccgagagttg gtgaacttgg gagtcgatcc cgaaaagcca atcgtggaaa cccaattcaa
     1441 ggtccgccca tcggaaccat tcgcgcggga cggcaacggg cgacaagaat cggaaaacgg
     1501 ccgcacgctc gagcatatca ctttctggga tccgcagtcc gacgtttgga ttgagcgcga
     1561 cgcctccgct ttgatcggca ccgtcacgag cggcagtcgt cccgtcaaga aaatcagagg
     1621 gttcgattat tttaccggca tgaccgggaa aggcggagcc aaggctcccg tgctctacca
     1681 cggcaaggag ttcaggattg acgtttataa tctggaagag gaacaaagag gctttcaccg
     1741 cggttgcgac gaggacgaga tttggtttca gtttcgcggc cattcgctca atcatacgga
     1801 gtggggcgcc atggagctgg acgccgggca gacggcgatg attcctcgtg gtattgctca
     1861 tcgaattacc ggcagcccag gttttctgcg catggtgttt tacagccgcg gtctggtcca
     1921 gccgaaagct ttcaataata tttccgaaag agaaacgagg tttgaggttc agtaacgtga
     1981 accaaaggta aacctggtgc atgaaactat ccgactggat ttctctcggg ttaagtgttc
     2041 tggcaacgtt atgtttacca agttttcttc cagccgcaga cggacagacg gaatgggagc
     2101 gaactctggc tgcggcgaag aaagaagggc caccagttat cggtatccct gctagcagtg
     2161 aattacggaa ggccatcggc acaaggttca aggagaagtt cggtatcgac atagagctct
     2221 ttccgagccg aggtccggag aacgtgacgc gcatcatcaa agaatacagc gccggtgttc
     2281 gctactttga cgtccttgtg gccggaggag cgacaccgct tgccatggtg gcagcggggg
     2341 cggcggacga ctttcagcct tacatgatcc tgccggaggt caaagaggca aaaaattggt
     2401 ggggcgggca tatctgggag gataacgtca gcacgaagaa atatatctac gcgtttcttt
     2461 gttacacgag cgagaccttc tggcacaaca ccagtcaggt agaaccgcag gagatgcgct
     2521 ccttcgacga tcttttcaac cccaaatgga aaggccggat cggttttctc gacccgcgga
     2581 atcctggttc agggcaaaac acttgggctt ttctctggaa agtaaaagga gaagaatttc
     2641 tcggcaagct ggtgcagcaa gatctcctga tcactcaaaa ccaacggcaa atcgccgacg
     2701 cgttggccaa aggaaagctc gcttttacga tcggtctcag tcattacagc tacgaaccgt
     2761 ttataaaagc tgggctgccg gtcaaaccgg tggccaatat caaggaggga gcccacgcca
     2821 acaacggcag cggtgtcgtg acggtcgtga aaaatcctcc gcatccaaac tctaccaaag
     2881 tattcattaa ttggttgctt gggaaagaag gacaggaact ctacggcaag gcgatgctcc
     2941 agggaacgcg acggctcgac gtggatacgc aatggctgaa ggagtcaggt atcgaggcgt
     3001 gcaaggacat catgacggtg gaggattatt atcgccggga aacccacctg gaatcttcgg
     3061 tcaacaagct caggaagccg gcgacggccc tggctgagaa acttctgagt aggaattgaa
     3121 ggaggtgagc tgtttacaat taggacgggg gcttaaagcc atgctgcggc tgtggattta
     3181 aaatcgttac gtcttaaaat ccggatagcc tgccctggcg gctttttctt tgtaatcttt
     3241 gactaactct tctctcggtt tctcgtggat ccaaaccgct tcggtgaaac tccagagcgt
     3301 gaacggatgg gcgcccaggg cgtaaagctt gccgtagtcc ttggtcgcca gcgcttcgcg
     3361 ctcctcgggc gtgaatttta gcttctggct cttctcccat ttctcaacaa acccgcgcgg
     3421 gttgctcacg tattcatctt cggctgattc gctcatgttc acgtggtgga tgagtttgtt
     3481 catcaggtat ttgctcatag taactcctcg tttaggcttt gattagcgtc caaatgccga
     3541 tggcaataaa accaatcccc gccaggtaat gtaacgtctt ttcactaacg tgttgcgaaa
     3601 tggcagaacc cgcggcgacg ccgatggcgg atgtcacaat caatgccaat gccgctccga
     3661 cgaaaacggt cagcttgctg gcgtctttgt ccgaagcgaa cagcattgtg gcgagctgtg
     3721 ttttgtctcc aagctccgcg atgaagaccg cagtgaatac ggtcgataat attttccaat
     3781 c
//
LOCUS       DASSWZ010000073         5935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1176582, whole genome shotgun sequence.
ACCESSION   DASSWZ010000073 DASSWZ010000000
VERSION     DASSWZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176582"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFN58_01755"
     CDS             complement(<1..238)
                     /locus_tag="VFN58_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET8916743.1"
                     /translation="MRSREKVTNRFIPQPSPRLQPPLYAILDPEQTKGRATETVLREL
                     LEGGIKLLQLRAKAMIPVDFLRLACATRALTRSYT"
     gene            complement(213..1658)
                     /locus_tag="VFN58_01760"
     CDS             complement(213..1658)
                     /locus_tag="VFN58_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8916744.1"
                     /translation="MDQGKILVTEDEESLRFVLKKALENEGYWVQTTANGDTARQLLT
                     ETHFDVSLMDIKLPDADGLTLLKEIKEAGIDTAMILMTAQNTMRNAIGAMKNGAFDYI
                     TKPFDLDEVLVLVKRAIDSRKLSRDFRELKEEVKKRFEPGVNIIGTSSSMQKVYKTIG
                     QVVDTQATILIQGESGTGKELVAKTIHYNSPRWNQPFVAINCAAIPRDLLESELFGHE
                     RGAFTGALDRRIGKFELAEGGTLFLDEVGDIPLELQTKLLRVLQDREYSRVGGRDVLK
                     ADVRILAATNQDLERAVKEKRFREDLYFRLKVIPIYLPPLRERRGDIPLLISYFIDKI
                     NREMGIQTSGISPEAQKLLEEHSWPGNVRELENTLIRAAVLSSGPILFPKDFTLQNKP
                     AVANLEVDQLSLEEIIRHKLEDYFRRTGDVDLDNLYSLVIERIERPLIELTLKKTRGN
                     QIRAAQILGINRNTLRKKIVDLRIEIKRESN"
     gene            complement(1664..2140)
                     /locus_tag="VFN58_01765"
     CDS             complement(1664..2140)
                     /locus_tag="VFN58_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8916745.1"
                     /translation="MPVNIHEVLDDVLLLETQAAEQLSIAVKKRFDPSLPPIRGDRAQ
                     LTQVFLNLVKNAFQAMNEGGILTITTRLETDFHIRGEGSHRSRLIWVDIEDEGVGIKE
                     EDLPHIFSPFFTTKHSGTGLGLAVCYRIVKEHGGLIRVESAEGRGTTFRISLLVAN"
     gene            complement(2131..2772)
                     /locus_tag="VFN58_01770"
     CDS             complement(2131..2772)
                     /locus_tag="VFN58_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET8916746.1"
                     /translation="MKVLNLHSKQSPSKISWEQILAGLEDAVITVDRDGKVSYFNEAA
                     EILTELSASQAIQKSVIQLFKREHWLIELVKKSQSPRQESTRGEGDLVTRWGHKVPVS
                     LTVSPLQDRHGNFLGSILVLRDLKHRKELEEDLKRADRLAMVGTLAAGLAHEIRNPLG
                     GIKGAAQLLKRSLDRDSSLLDFANIMIREVDRVTNSLSSFLTYPARQNCNSCP"
     gene            2952..3419
                     /gene="rimP"
                     /locus_tag="VFN58_01775"
     CDS             2952..3419
                     /gene="rimP"
                     /locus_tag="VFN58_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET8916747.1"
                     /translation="MDATLTRVWQLAAPLVEGERMEIVDIEFRYEGSRGGRVLRLYLD
                     KEGGPNVDDLSRVSRQLSELLDGEDTIDGTYTLEVSSPGINRPLKKPAHFARFVGKRI
                     RVRTRDLIDGRRSFLGILGQVVEDSVILTQEGKRYQIPFSMIEKSNYDHDWSA"
     gene            3421..4758
                     /gene="nusA"
                     /locus_tag="VFN58_01780"
     CDS             3421..4758
                     /gene="nusA"
                     /locus_tag="VFN58_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET8916748.1"
                     /translation="MQQDLNRVIEQVSKEKGIDKSILINALENAMISAAKKTFGHQRK
                     IEAQFNPEIGEVELFEAKTVVENVQDPAVEVTLQEAREGLDPDAEIGDELLSKLDSSM
                     FGRIAAQAAKQNIVQRVRDAEREIIYNEFKGREGQLVNGIVQRFEKRNIIVNLGRTDA
                     ILPEKEQVPRERYRQGDRIRAYIVSVEITSRGPQIVLSRTHPGMLTKLFEQEVPEIYE
                     SIVEVKGAAREPGGRAKIAVVSNDPDVDPVGACVGMKGTRVQAVVQELRGEKIDIVHW
                     IPDQAEYVCRALAPAKVSKIIIDDDEHSMEVIVPDDQLSLAIGKKGQNVRLASRLTGW
                     RIDVRSEAEAEEETRRARQSIGAIPGINDLVAELLYQAGFKSAEEIADSELEEILDVE
                     GVSKDKAEALYKSAKEYVAEKRKKEQEAQAAAQAAAAAVSEVQPVEEKATQES"
     gene            5043..>5935
                     /locus_tag="VFN58_01785"
     CDS             5043..>5935
                     /locus_tag="VFN58_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HET8916749.1"
                     /translation="MGKTRVHLLAKELGIETKDLITQLDKLGIRGRKAQSALEDDEVT
                     RIRAALAGQDKPQVHVGEEKVVADRVVTAEDENLGEIQARETVVERRVRANVIRRRTS
                     RVEVVSQAGEKPDAEVVETGPEKTPASAIEETAPIAPAEEFPEGPGAEIEIPDQLTGE
                     MEAVFQEEPPSAEEMITEAAPSAQPVPEESAKPATEEKPTPVQEAPRGARILGRIDLR
                     RTTRVEPAPTPVARRPVPGAPAAPSVVPTEALRPARDGEAAKPQGAEGEKPAKPGRHK
                     KRVVKKQDVLEFREKELRSGR"
BASE COUNT         1388 a         1519 c         1752 g         1276 t
ORIGIN      
        1 aggtgtatga tcgagtgagt gcgcgcgtgg cacaggcgag tcgaagaaag tcgacgggaa
       61 tcatcgcctt ggcacgcaat tgaagaagct tgataccgcc ttccagcagc tcacggagca
      121 cggtctcggt ggcgcggccc ttggtttgct cgggatcgag aatggcgtaa agcggcggct
      181 gaagccttgg tgaaggctga ggaatgaagc ggttagttac tttctctctt gatctcaatc
      241 cgcaggtcga cgattttctt gcgcagggtg ttgcggttga tgccgagaat ctgcgccgcg
      301 cgaatttgat tcccacgggt tttcttaagc gtcagctcaa tcaacggccg ctcgattctt
      361 tcgatcacta aggaataaag gttatcgaga tccacgtcac cggttcggcg aaaatagtcc
      421 tcgagtttgt gacggataat ctcttcgagg gacaactgat ccacctccag gttggcgacg
      481 gcgggtttat tctgcagggt gaaatctttg ggaaacagga tcgggccgga ggagagcacg
      541 gcggcccgga tgagcgtgtt ctccagctcg cgaacgttgc cgggccaaga atgctcttct
      601 aaaagtttct gcgcctccgg agaaatgccg gaggtctgaa tgcccatctc gcggttgatc
      661 ttatcgatga agtagctgat caagagtggg atatcgcccc tgcgctctcg caacggcggc
      721 aagtagatcg ggatgacttt gagacgaaag tagagatctt cacggaagcg tttctctttg
      781 accgctctct caagatcttg gttggtggct gccagaatcc gaacatcggc cttcaacacg
      841 tcccggccgc cgacccggct atattcccga tcctggagca ctcggaggag tttggtttgg
      901 agttccagcg ggatgtcgcc cacctcgtcg aggaaaagag ttccgccttc ggccaattcg
      961 aatttaccga tgcgccgatc caacgcgccg gtaaaagcgc ccctttcatg tccgaacagc
     1021 tcgctttcca acaagtcgcg cggaatggcg gcgcagttga tggccacaaa cggctgattc
     1081 cagcgcggcg aattgtaatg aatcgtcttg gcgaccagct ctttacccgt gccgctctcc
     1141 ccctgaatta gaatcgtggc ttgtgtgtcg accacctgcc caatggtctt gtagaccttc
     1201 tgcatactgg agctcgtgcc aatgatattg acgccgggct caaaacgttt tttcacttct
     1261 tctttgagct ctcggaaatc ccggctcaac ttgcggctat cgatagcccg tttgaccagg
     1321 accaagacct cgtcaagatc aaagggcttg gtaatgtaat caaaggcgcc gttcttcatg
     1381 gcaccaatgg cattgcgcat ggtgttctga gcggtcatca gaatcatcgc ggtatcgatg
     1441 cccgcttctt tgatttcttt cagcaaggtc aggccgtcgg cgtcgggcaa tttaatgtcc
     1501 atgagcgaga cgtcgaaatg ggtttccgtc agtagctggc gcgccgtgtc gccattagcc
     1561 gtggtttgca cccagtagcc ctcgttctcg agggctttct tcagcacaaa gcgcagcgat
     1621 tcttcgtctt ccgtaaccag gatctttccc tgatccataa taactagttc gccaccagca
     1681 gagaaatcct gaaggtggtt cctctcccct cggcgctctc gacgcggatg agcccgccgt
     1741 gctctttgac gattcgatag cagaccgcga gcccgagacc ggtaccgctg tgttttgtgg
     1801 taaagaaagg agagaagata tggggcaggt cttcctcttt gattccgact ccctcgtcct
     1861 cgatgtccac ccaaatcaac ctgctgcgat ggcttccttc accgcgtatg tgaaaatcgg
     1921 tttccagccg tgtcgtgatt gtcagaatgc cgccttcgtt catcgcttga aaggcatttt
     1981 tcaccaagtt gagaaaaact tgtgtcagct gtgctcggtc gccacggatc gggggcaggc
     2041 tgggatcgaa acgtttctta accgcgatgc tcagctgttc cgccgcttgc gtctcgagca
     2101 atagaacgtc atccaacacc tcgtggatat tcacgggcat gagttgcagt tttgccgggc
     2161 gggataggtc aagaagctgc tcaatgagtt tgtcacccga tcgacttcgc gaatcatgat
     2221 gttggcaaag tccagcaagg atgaatcgcg gtccaaggag cgcttcagca actgagcggc
     2281 gcccttgatt cctcccaggg gattacgaat ttcatgagcc aggccggcgg ccaaggtccc
     2341 gaccattgct aaacgatccg cccgtttcag atcttcctct agttctttcc gatgcttcag
     2401 atcccgtagg acaaggatgc tacctagaaa attgccgtgg cgatcttgca gaggagaaac
     2461 cgtgaggctg accggcacct tatgtcccca acgggtcacc aaatcccctt cgcctcgcgt
     2521 gctctcttgt cgtggcgact gactcttctt gaccagttcg atgagccaat gctcccgctt
     2581 gaagagttgg atgaccgatt tttgtatcgc ttgagatgcg gaaagttccg tgaggatctc
     2641 ggcagcttcg ttgaaatagg agaccttgcc gtcccggtcg acagtgataa cggcatcttc
     2701 caggccggca aggatctgct cccaggagat tttcgacggt gactgcttgc tgtgaaggtt
     2761 taaaactttc atcgcggcga catgcgtagt ccaaaaatca tagcaaatct agctcggcaa
     2821 gcaaatgcac cacgcacggc ttcgggcctt gcaattccaa cgttcactat ggtaaaaaca
     2881 tcttacactt taggagttta cactcgaagt gggctatgcc cgcttttttt gtttctggga
     2941 ctggaattgt gatggatgcg accctcacgc gagtttggca gctcgcggcc ccgctagtcg
     3001 aaggcgaaag aatggagatt gtggatattg agtttcggta cgagggaagc cgcggcgggc
     3061 gggtgctacg gctgtatttg gacaaagagg gggggccgaa tgtcgatgat ttgagccggg
     3121 taagccgcca gttgagcgag ctgctggacg gggaagatac gatcgacggc acctataccc
     3181 tggaggtttc ctcccccggg atcaatcgcc cgctcaaaaa gccggcgcat tttgcgcggt
     3241 ttgtcggcaa gcgaattcgc gttcgcaccc gggatttgat cgatggccgg agatcctttc
     3301 taggaattct cggccaggtc gtagaggaca gtgtgatttt aacccaagaa gggaaacgct
     3361 atcagattcc attttccatg attgaaaagt cgaattacga tcacgattgg agcgcttaat
     3421 atgcagcagg atctcaatcg agtcattgaa caagtgagca aagaaaaagg catcgacaag
     3481 tctattctca tcaacgcttt ggaaaatgcc atgatctccg cggccaaaaa gaccttcggt
     3541 catcaacgaa agatcgaggc ccaattcaat cctgagattg gcgaggtcga gctctttgag
     3601 gccaagacgg tggtggaaaa cgtgcaagac ccggctgtgg aggtaaccct gcaggaagct
     3661 cgagagggtc ttgacccaga tgccgagatc ggcgacgagc tcctgagcaa actggactcg
     3721 agtatgttcg gccgtatcgc cgcacaagcc gccaaacaaa atattgtcca acgcgttcgg
     3781 gatgcggagc gggagattat ctataacgag tttaaggggc gggaaggaca gttggtcaat
     3841 ggcattgtgc agcgcttcga gaagcgcaac attatcgtga accttggacg caccgatgct
     3901 attcttccag aaaaggagca agtgccgagg gaacgctacc gccaggggga tcggattcga
     3961 gcctacatcg tgagcgttga gatcacgagt cgcgggccgc agatcgtcct gtcgcgcacc
     4021 catcccggca tgctcacaaa actttttgag caggaagttc cggaaatcta tgaaagtatc
     4081 gtcgaggtta agggcgcggc gcgtgagccc ggaggccggg cgaagatcgc cgtcgtgtcc
     4141 aatgatccgg acgtggatcc cgtgggggcc tgtgttggca tgaaggggac tcgggttcag
     4201 gcggtggttc aggagttacg cggtgagaaa atcgacatcg ttcactggat accggaccaa
     4261 gccgaatatg tatgccgcgc cctggctcca gcgaaagttt ctaagattat catcgatgac
     4321 gacgagcaca gcatggaggt tattgttccc gatgatcaac tgtctctcgc cattggtaag
     4381 aagggccaaa atgtgcgctt ggcgtcgcgc ttgaccggct ggcggatcga tgtgcgtagc
     4441 gaagctgagg ctgaagaaga gacgcgccgg gcgcgccagt cgatcggcgc cattcccggc
     4501 attaacgact tggtcgccga gttgctttac caagccggat tcaagtctgc cgaagaaatc
     4561 gctgacagcg aattggaaga gatcctcgat gtcgaaggtg tcagcaagga taaagccgag
     4621 gcattgtaca agtcggccaa ggaatacgtc gccgagaaac gaaaaaagga acaagaggcg
     4681 caagccgcag cacaagcggc ggcggccgct gtcagcgaag tgcagccggt ggaggaaaaa
     4741 gcaactcaag aatcctaggt gcgcctttgt cgaaacgagg ccatcgtcca gaacggacat
     4801 gcctgggctg tggtgcgagg gaagagcaga ataaattgat tcgcctgatg gttggagagc
     4861 agggtgagct gaaaatcgat cggttggctg acggtcgggg aggatatctt cacccggctc
     4921 gttcatgttg gcagccattt ttgcggaaaa agagccttca tcgagcattt cacgtggaaa
     4981 tcggcaaaga ggcaagggaa aaaataattc gagaattgca ggagcgatac ggggagtaag
     5041 agatgggaaa aacgagagtc catttactgg ctaaggagct tggaatcgag accaaggatc
     5101 tcattaccca acttgacaaa ttgggaatcc gcggccgtaa ggctcagagt gccctggaag
     5161 atgatgaagt aactcgcatc agggcggctt tagcaggaca agataagcct caggtccacg
     5221 ttggcgaaga aaaggtcgtc gccgaccgtg tggtgactgc agaggatgag aaccttggag
     5281 aaattcaggc tcgggaaacg gtggtggagc gcagagttcg agccaacgtc attcgtcgcc
     5341 gtaccagccg ggtcgaagtc gtttcgcaag cgggtgagaa acccgacgcg gaagtcgtcg
     5401 agaccggccc ggaaaaaaca cccgcctcgg ctatcgaaga aacggcgccg attgcacccg
     5461 ccgaggaatt cccagaggga cctggggcgg aaattgaaat tccggatcag ctgaccggtg
     5521 agatggaggc ggtttttcag gaagaaccgc ctagcgcaga ggagatgata acggaagccg
     5581 cgccttcggc ccagccagtg ccggaagaat ccgctaagcc tgccacagaa gaaaagccca
     5641 cacccgttca ggaggcgccc cgcggggcgc ggattcttgg ccgcattgat ctcaggcgaa
     5701 caacaagagt cgagccggcg ccgacacctg tagctcgacg tccggtgcca ggcgcgccgg
     5761 ctgcgccctc agttgttcca acggaggctt tgagacctgc tcgggatggt gaggcggcaa
     5821 aaccacaggg agccgaaggg gagaaaccag ccaagccggg ccgccacaag aagcgcgtcg
     5881 tcaagaaaca agatgtcttg gagtttcgag aaaaggagct tcgctccgga cgtat
//
LOCUS       DASSWZ010000074        14862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_2062, whole genome shotgun sequence.
ACCESSION   DASSWZ010000074 DASSWZ010000000
VERSION     DASSWZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14862
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFN58_01790"
     CDS             <1..187
                     /locus_tag="VFN58_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8916750.1"
                     /translation="ALGAKRAQAAKDYLITLGISTERLSTISYGEEIPVCKESNESCW
                     RQNRHARFVIIQARPAS"
     gene            230..1060
                     /gene="ybgF"
                     /locus_tag="VFN58_01795"
     CDS             230..1060
                     /gene="ybgF"
                     /locus_tag="VFN58_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET8916751.1"
                     /translation="MKAIHFCFLAVAAGWIVGCAHPQQIDLIEREQRRMRADTNAMQS
                     DLDSMRGTLADTRANMQQMQREVSALKERVDETRVQVGRQIGQTSREGDQRVKNLETR
                     LAKLDDDLKAQGEALKTREEAIKQLSETLEAQQKTVPSDGLAEGAAAESDAIRKDYEV
                     AWRTLDKKDYKAAIARFREFIKKYPKSALAGNAQYWIGESLYALKEFDQAIIEFDAVR
                     RRYPQGEKVPAALLKQGFAFAELGEKVNARLILQEVVEKYPQSPEAGRAKQRLKSLES
                     "
     gene            1173..2141
                     /locus_tag="VFN58_01800"
     CDS             1173..2141
                     /locus_tag="VFN58_01800"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET8916752.1"
                     /translation="MKIFRHIEDQGLSMSGTVATMGNFDGIHLGHQALVRNTVEESTR
                     LGCPSTVLTFEPHPLKVLAPERAPLLILSYDDKMALLQSLGVDIVIAQRFDRQFASIA
                     ADEFIYRFLVNRLKIRKLWVGRDLRFGQDRKGGTESLLRLAPDAGFEVSVLDPILLDG
                     IRISSSRIRQLIEEGRVDEVRSMLGRYHFVSGRVVTGHRRGQKLGFPTANIASQTEVV
                     PLNGIYATLIQLKNKQWLSVSSIGVNPTFGDGPRTLESFILDFDGDIYGEWLKLSFVK
                     RIRDEKKFVTVNDLVAQMHEDVKQAKAVFNELGLTDKEDPQSNSSL"
     gene            2138..3133
                     /locus_tag="VFN58_01805"
     CDS             2138..3133
                     /locus_tag="VFN58_01805"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET8916753.1"
                     /translation="MNLQKTVVEPQSAGIRLDLFLTRCLPDIMKGEEFSRSGIQKLIA
                     KGQITLNGKPAKASARLKTNDLVAIKTLPPKKSILVAEPIALNVIYEDDDCIVINKGP
                     GIVVHPAAGRTTGTLVNAILHHCPDLQGIGGESRPGIVHRLDKDTSGVMIIAKRMPAF
                     QQLARQFKERRVKKEYVALVLGKPKDRHGIIDRPIGRHRSDRKRMSSVHALPKKKEAV
                     TEWVVERSFRRKDGALSASWVSLLRLMPKTGRTHQIRVHLADMGYPLVGDKVYGRKRA
                     NPTAKNAAGSSLDSFSRQALHAERLSLLHPRTGAPMEFYAPLAEDIQNLLQTLEG"
     gene            3508..4809
                     /gene="rho"
                     /locus_tag="VFN58_01810"
     CDS             3508..4809
                     /gene="rho"
                     /locus_tag="VFN58_01810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET8916754.1"
                     /translation="MVRGRTRTAEPETLEDNGLNLKALKEKKISDLALIGKNFNIEGA
                     ANMRKQELIFAILQAQTEQNGHIYGEGVLETLPDGFGFLRAPDYNYLPGPDDIYVSPS
                     QIRRFNLRTGDIISGHIRPPKEGERYFALLKVESINYEDPERAREKILFDNLTPLYPN
                     ESFHLEYQPEDYTTRIVDLLTPVGKGQRGLIVAAPRTGKTMMLQHLAKAIAHNHKEVI
                     LIVLLIDERPEEVTDMQRSVDGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHGKDVV
                     ILLDSITRLARAYNTVVPPSGKILSGGVDSNALHKPKKFFGAARNIEDGGSLTIIATA
                     LIDTGSRMDEVIFEEFKGTGNMEVHLDRRLMDKRVFPAIDINKSGTRKEELLIAKEDL
                     NRIWILRKVLSQLSVVDAMEFLQDKMHGTKNNKEFLESMNQ"
     gene            5042..5833
                     /gene="rpsB"
                     /locus_tag="VFN58_01815"
     CDS             5042..5833
                     /gene="rpsB"
                     /locus_tag="VFN58_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET8916755.1"
                     /translation="MSDISMKQLLEAGVHFGHQTSRWNPKMKPYIFGARNGIHIIDLQ
                     QTVAMFKDLEAVVRNIAATGGHVLFVGTKKQAQEAVKEEAERCGMFHVQNRWLGGTLT
                     NFSTIRQSIERLRRLEEMENDPKIVDALTKKEMLGLKREKEKLEQSLGGIKSMRKLPD
                     AIFVVDPKQEEIAVKEARKLGIPVVAVIDTNCDPDLVDYKVPGNDDAIRAIRLFCSAI
                     ADAINEGKMLYEQSLVKDKADEKAASDVPTEEPSRATASQAMGGV"
     gene            5840..6448
                     /gene="tsf"
                     /locus_tag="VFN58_01820"
     CDS             5840..6448
                     /gene="tsf"
                     /locus_tag="VFN58_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET8916756.1"
                     /translation="MELNATVVKELREKTGAGFLDCKKALAETGGNLEKAVDYLRQKG
                     LAAAAKKADRVAADGAVGAYVHPGGKIGVLVEINCQTDFVARTTEFQTLLKDMAMQIA
                     AASPRYVKREDVSVDEIEKEREIYRGQALESGKPEKVVEKIVEGKMERFYSEVCLLEQ
                     AFIKDPDRKVSDTINDAITRLGEKIEVRRFTRYHLGEGVQKD"
     gene            6466..7197
                     /gene="pyrH"
                     /locus_tag="VFN58_01825"
     CDS             6466..7197
                     /gene="pyrH"
                     /locus_tag="VFN58_01825"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET8916757.1"
                     /translation="MNNGSPKYKRIILKVTGEVFAGPHKFGIDGKTVKAFAQEIQEVK
                     EMGCELALVIGGGNIFRGAVASEIGMDRASADTMGMLATVINSLALQDALEKLGVSTR
                     VLSAIEMRQVAEPYIRRRATRHLEKGRVVIFAGGTGNPYFTTDTTASLRAMEVGAEVI
                     LKATKVEGVYDADPLKHAGAHKFEELTYIEVLNRELKVMDSTAISLCMDNQLPIIVFN
                     IMEKGNIKRVVSGEPIGTLVSGGKR"
     gene            7194..7751
                     /gene="frr"
                     /locus_tag="VFN58_01830"
     CDS             7194..7751
                     /gene="frr"
                     /locus_tag="VFN58_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET8916758.1"
                     /translation="MNEAIFTQLQKEMDQSVGALRKDLAKIRTGRASTALLEHVVVDY
                     YGATTPLNQLATLSAPEPRLLVVQPYDRSAMGNIEKAILKSDLGLTPNNDGKIIRVPI
                     PQLTEERRKELVKHVKKIGEEFRVSVRNHRRVAVEHLKEAEKKKEITADDSKHGHDRV
                     QKITDDFIGRIDKIIKTKEEEIMEV"
     gene            7789..8643
                     /locus_tag="VFN58_01835"
     CDS             7789..8643
                     /locus_tag="VFN58_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET8916759.1"
                     /translation="MSFVAEPLQAFATRSIHETDAARLTMDLSGLDKSRLPQHVAIIM
                     DGNGRWAKERGKSRIEGHRRGKTSVRVIVEMSRKIGIPFLSLYAFSTENWLRPQDEVH
                     ALMGLLEHYLAAEQAKMMRYSIRLVAIGDRSRLPISVRRTLERVIDLTRDNKRMTVIL
                     ALSYSGRNEIVRMMKRIASEVREGTYDPEEIDEKTIAAHMDTPDVPDPDLLIRTSGEL
                     RISNFYLWQIAYSELYVTSTLWPDFREKDYLQALIEFQQRRRRFGRTDEQVMDPAPSS
                     LEASGIKS"
     gene            8627..9433
                     /locus_tag="VFN58_01840"
     CDS             8627..9433
                     /locus_tag="VFN58_01840"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET8916760.1"
                     /translation="MESSLRSRVLTASVGIPLLIVLVGWGPSWAFVGFFVVLNAAALR
                     EYFAMAFPGRMREQLWGILFGLSLSLALLFSQASKGEVGLSVLFVFFFSIYLFMAGKL
                     SERLNRLAWTLLGAVYLGYLLPHWVLLFRMPHGRAWVFFVLLVIMMGDTAAYVTGTYF
                     GKRKLAPEISPGKTIEGTLGYVAGSVAGGCLGASFLVTELGAIEVVALSGMLSILGQI
                     GDLFESLIKRVFAVKDSGTVLPGHGGLLDRLDSLIFPAVFATTYLKVFHP"
     gene            9430..10578
                     /locus_tag="VFN58_01845"
     CDS             9430..10578
                     /locus_tag="VFN58_01845"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET8916761.1"
                     /translation="MRTIALLGSTGSIGLSTLDLVRRFPERFKVYGLVAGENLKRLMS
                     QIREFAPKCVAIKNEEDVPRLRKLLGDNRVEVLCGERGAAAVSTAHEVDIVVAAIVGG
                     AGLVPTLAAVRAGKEVALANKEALVMAGEIFVSTAKQKGVRLLPVDSEHSAIFQCLQG
                     NRRDEVDKLILTASGGPFLRTPLKLLNRATVAQALKHPNWKMGRKITIDSATMMNKGL
                     EVVEARWEFDMDPSRIDVVIHPQSVVHSMVRYQDGSIIAQLGIPDMRIPIAYALTYPH
                     RLKGGWKPLDLTQHGELSFLPVDSKRFPALRLAYAALKEGGTMSAVLNAANEVAVAAF
                     LERKIGFRDIHRIIDKTMQGHAPRRPKEVDEVLEVDRWARQTASSLIH"
     gene            10606..11688
                     /gene="rseP"
                     /locus_tag="VFN58_01850"
     CDS             10606..11688
                     /gene="rseP"
                     /locus_tag="VFN58_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET8916762.1"
                     /translation="MENVIYSIVAAIIGLGVLIVIHEFGHFLFAKLFGVGVLTFSVGF
                     GPKLWVKKKGETEYALSAFPLGGYVKMVGEDPDEEVQQADVEKSFAHKGLLRRMAIVA
                     AGPGFNLLLAVFLLMIVFIFYGVPVLSTQVGGVEPGSPADRAGIKKGDRIVAVSGQPV
                     TEWEELSKRIKESQGSPLNLSIRRDAQDVALTVEPTKRESRTIFGEKKDEWMIGIGSH
                     VSIEKGNPGLAIVKAVQQTYDYAKLTLVALYKMISGEVSPRNLGGPILIAQMAGQQAQ
                     EGLGSFLGFLAILSINLGVLNLLPIPVLDGGHLLFFLVEAVIGKPVAVRHREVAQQVG
                     IFLLMLLMIYAFYNDIARFFEKQVGG"
     gene            11685..12419
                     /gene="tsaB"
                     /locus_tag="VFN58_01855"
     CDS             11685..12419
                     /gene="tsaB"
                     /locus_tag="VFN58_01855"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET8916763.1"
                     /translation="MRVIGIDTASGAASVALVENGLLLSEKTDLRCESARDAIRRGGK
                     SNRAETLLPLIELLFESTGVSLQNITGFALSIGPGSFTGLRIGLSTVKGLAYGWQIPV
                     VGVSTLFAHAARVTDYEGLICALLDARKNEVYAAVFRKTADIVNRVTEDVVASAANVV
                     EMVRGLQRGSPCLFVGDGAVVYKHLFLELPGVCLPETTNYPTVAAAVARLGEDRFRSN
                     RVDDLGALTPAYVRPSEAEFKRGSSL"
     gene            12521..12757
                     /locus_tag="VFN58_01860"
     CDS             12521..12757
                     /locus_tag="VFN58_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HET8916764.1"
                     /translation="MEVKEEQVIASLVDKDPELKKYYEEHQELERKLAEFQHKHHLSP
                     DEEVEKKRLQKLKLLGKDKIMEILGRHRQAGATQ"
     gene            12767..14545
                     /gene="ilvB"
                     /locus_tag="VFN58_01865"
     CDS             12767..14545
                     /gene="ilvB"
                     /locus_tag="VFN58_01865"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET8916765.1"
                     /translation="MKKTGADIFVESLRHEGVKTVFGLPGGVVLKIFDVLCQQKDIEL
                     VLTRHEQGAAHMAEGYAKATGKPGVVLVTSGPGMTNIVTGLADAYMDSVPLVAFTGQV
                     STNLIGNDAFQEADNVGISRPCTKHNVLVKDVNDLAQTIKEAFYIAMSGRPGPVLVDI
                     PKDVSTEKAEFKYPDKVNLRGYNPTISGNKHQIKQAAEEIMKAKKPMIYVGGGAIFSD
                     AADEIRELAELTQIPVTMTLMGLGSFPGTHPLALGMLGMHGGYWTNMAMHYSDLLIAV
                     GARFDDRVTGKLSEFCPEARVIHIDIDPTSIKKTFHAHIPIVGDVKAVLRQMNVMLRS
                     MDGNASEVKALRAPWLKQIYEWKKGHPLTYKQDSKITKPQFVIERLFELTKHNAIVAT
                     DVGQHQMFAAQYFKGTKPRTWLTSGGLGTMGFGFPAALGAQKAFPEKLVLCITSEGSF
                     QMNLQEMATAVEHKLPVKIVLLNNGFHGMVRQWQDLFYEGRYSASYLGKLPDFVKLAE
                     AYDILGLRAEKPREVESVLKEGLKHKGPALMDIHTDPFENCYPMIPAGGAHHEMMLED
                     PPELTQAKKGGSHKRKVDEGEGVLPA"
     gene            14558..>14862
                     /gene="ilvN"
                     /locus_tag="VFN58_01870"
     CDS             14558..>14862
                     /gene="ilvN"
                     /locus_tag="VFN58_01870"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET8916766.1"
                     /translation="MEHIISVLVENKFGVLARVAGLFSARGYNIESLSVAPTLDPTTS
                     MITIVTAGDDPIIEQIMKQLNKVVEVLKVVDLTETDFLDRETALIKVHTKPEHRDEA"
BASE COUNT         3717 a         3522 c         4248 g         3375 t
ORIGIN      
        1 ggctctgggc gcaaagagag cccaggcagc gaaggattat ttgatcacgc tggggatctc
       61 taccgagcgg ctatcgacca tcagttatgg cgaagagatt ccggtgtgta aggagtcaaa
      121 cgagagttgc tggcgtcaaa accgacacgc acgttttgtg attattcaag cccgacctgc
      181 ttcataaatg ggttaattct cggctagctc cctcgtgttt cgtcttttta tgaaagcgat
      241 tcatttttgt tttttggccg ttgccgcggg ttggattgtt ggctgcgcgc atccccaaca
      301 gatcgatctc atcgagcgcg aacagcggcg gatgcgcgcc gatacaaacg ctatgcagtc
      361 ggatttggat tcgatgcgag ggacccttgc cgacacccgc gccaatatgc agcagatgca
      421 gagagaggtg agtgcgctca aggaaagggt cgacgaaacc cgagtccaag tgggtaggca
      481 aatcggccaa accagtcgtg aaggggatca aagagtaaag aatttggaaa ctcggttggc
      541 gaaacttgac gacgacctga aagcgcaggg agaggcgttg aaaacgcgtg aggaggcgat
      601 caagcaactc agtgagactt tggaggccca acagaaaacg gtgccgagcg atggccttgc
      661 cgagggagcg gcggcagagt ctgacgcgat caggaaagat tacgaagtcg cctggcgcac
      721 attggacaag aaagattaca aggcggccat tgcccgattc agggaattca tcaagaagta
      781 tccgaaaagt gcgcttgcgg gaaatgcgca atattggatt ggtgaaagcc tgtacgctct
      841 gaaagaattc gaccaagcga ttatcgaatt tgatgcggtc cgaagaagat atccgcaggg
      901 agaaaaagtt ccagccgcgc ttttgaagca aggctttgcg ttcgcggaac tgggagagaa
      961 agtcaatgct cggctcattc tccaggaggt agtcgagaag tatccacagt ctcccgaagc
     1021 gggaagagcc aaacaacgat taaaatcgct agaatcgtga ttaggcaacc agcctgattc
     1081 catagactcc gttacaactt tactgccggt cgccccctcg tttcccagtc gatgtcccgt
     1141 agttgttcct tctgggatga gggacggaaa caatgaaaat ctttcgtcac atagaggatc
     1201 aagggctttc aatgtcagga acggtcgcta cgatgggtaa tttcgacggt atccatcttg
     1261 ggcaccaagc tctggtgcgc aatacggtgg aggaatcgac gcggctgggt tgtccttcga
     1321 ccgttttgac gtttgagccc catccgctaa aagttcttgc tccagaacgc gctcctttgc
     1381 tcattctctc ttacgacgac aaaatggccc ttcttcaatc cttgggtgtc gatattgtta
     1441 tcgcccagcg gtttgaccgc caattcgcaa gcattgcagc agatgaattc atctaccggt
     1501 ttcttgtgaa tcggctaaag attaggaaat tatgggtggg gcgagacttg cgctttggtc
     1561 aggataggaa agggggaacg gagagtctgc tgcggttggc accagacgcc ggattcgagg
     1621 tgagcgtgtt agatcccatt ttgctcgacg gcatccggat cagcagctcg agaatccgtc
     1681 agttgattga agaaggtcgc gtggatgaag ttcgctccat gctgggacgc tatcactttg
     1741 tatcgggtcg cgtggtcacg gggcaccgcc gaggtcagaa actaggattt ccgactgcga
     1801 atattgcgag ccagacggag gttgttcctt tgaacgggat ctacgccacg ctaatccaac
     1861 tcaagaacaa acaatggttg agtgtatcta gcatcggcgt taacccgacc tttggcgacg
     1921 ggccgagaac attggaaagt tttatcttgg attttgacgg cgacatatac ggcgagtggc
     1981 tcaagctttc cttcgtgaag agaatccgag atgaaaaaaa gtttgtgacg gtcaacgatt
     2041 tagttgcgca gatgcacgaa gacgtgaagc aggcaaaagc cgttttcaat gaactgggat
     2101 tgacagacaa agaggatccc caaagcaatt catctttatg aatcttcaaa aaacggtcgt
     2161 ggaacctcaa tcagccggta tcagactgga tcttttctta acccgttgtt tgcccgacat
     2221 catgaaaggc gaggagttta gccgttccgg gattcaaaag ttaatcgcca aaggacagat
     2281 cactctaaac ggcaaacccg cgaaagccag cgcgcgactg aaaaccaacg atctcgtcgc
     2341 catcaagacc ttgccgccga aaaagtctat cttggtggct gagcctattg ccctgaacgt
     2401 tatctacgag gatgatgact gcatcgttat caacaaaggt cctggaatag tggttcaccc
     2461 ggcggccggc agaaccacag gaactttggt taatgcaata ttgcatcact gcccagacct
     2521 ccaagggatc ggcggagaat cccgtccagg aatcgtccat cgattggaca aagatacctc
     2581 tggagtcatg atcatcgcta aaaggatgcc tgcctttcaa cagcttgcgc gacagttcaa
     2641 agaaagacgg gtgaagaagg aatacgttgc gcttgtgttg gggaaaccga aagaccggca
     2701 cgggatcatc gatcgaccca taggacgaca ccgttcggat cggaagcgga tgtccagcgt
     2761 tcacgctctg cctaagaaaa aagaggccgt gacagaatgg gttgttgaga gatcctttcg
     2821 acgaaaggat ggggcactga gcgcttcttg ggtgagtttg ctgaggctca tgccgaagac
     2881 cggacgaact catcaaatcc gggtccatct cgccgatatg ggttatccct tggtcggcga
     2941 taaggtctac ggccgcaaac gggcgaatcc aacggccaaa aatgccgctg gttccagttt
     3001 ggactcattc tcgcggcagg cactccatgc ggagcggctg agcctgttgc atcctcggac
     3061 tggagctcca atggaattct acgcgccgct ggccgaagac atccagaacc ttctgcaaac
     3121 tctcgaaggt tgacatctgg agcaccgaag gcgcgcgaaa tcaactttgg gggttgacaa
     3181 ggagagaggt tttacttagt attgaattaa gaaattgttc atttcagcga tctctttgaa
     3241 ttaaccccca cactgacaaa atcgttaagc atccggcctg gatttgtttt ggatcttaag
     3301 gaaaagattc gcatccgatt gtagaaaccc gtcgcattga catgtcgcgt ctgcaaacgt
     3361 gccgctactt tcgtaatctc ctctcatcga gaggagttgg aatttctgaa tcggcagcac
     3421 aattctgcgg gtaagtacgg agaagatgcg gtgtcggcaa aatatcacga acgttccttc
     3481 aagagtgtga aaggagaggc atagtttatg gtccgtggca gaactcgaac ggcagaaccg
     3541 gaaaccctgg aagacaatgg gttaaacctc aaggccctca aagaaaagaa gatcagcgac
     3601 ctagcactga tcggcaagaa ctttaatatt gagggcgccg ccaatatgcg caagcaagag
     3661 ctgatttttg cgattttgca agcgcaaacg gagcagaacg gccacatcta cggggaaggc
     3721 gtgctggaaa ccctacccga tggttttggt tttctgcggg ctcccgatta caattatctt
     3781 ccagggcctg atgatattta cgtttctccg agccaaattc gtagattcaa cttgcggaca
     3841 ggcgatatta tctccggcca cattcgccct cccaaagagg gggaacgtta cttcgcgctc
     3901 ctcaaggttg aatcgatcaa ctacgaagat ccggaaagag ctcgggaaaa aatccttttc
     3961 gataatctta cccctctcta ccccaatgaa agtttccatc tcgaatatca gcccgaagat
     4021 tatacgacgc gtatcgtgga tttgttgaca ccagttggca aaggccagcg tggcttgatc
     4081 gtcgccgcgc cgcggacggg caaaacgatg atgctacagc atctcgccaa ggccattgcc
     4141 cacaatcaca aagaagtcat cctcatcgtt ctgctgattg acgaacgtcc cgaagaggtc
     4201 acggatatgc aacgatcggt ggatggggag gttatcagtt ctactttcga tgagcctgcg
     4261 acacgacacg ttcaggttgc cgaaatggtg attgaaaaag ccaaacggct tgtcgaacat
     4321 ggcaaagacg tggtgattct tctcgacagc ataacccggc tcgcgcgggc gtataacacg
     4381 gtagtgccgc caagcggcaa gattctatcc ggcggtgtcg actccaatgc gcttcataag
     4441 ccgaagaagt tcttcggtgc ggcgagaaac atcgaggatg gtggcagcct gactattatt
     4501 gccacggccc tgatcgatac cggtagccgg atggatgaag tcatttttga agaattcaaa
     4561 ggcactggga acatggaagt gcacttagat cgacggctca tggataagcg ggttttccct
     4621 gcaatcgaca tcaacaagtc gggaaccagg aaggaggaat tgctgattgc caaagaggac
     4681 ttgaatcgga tctggatctt gcggaaagta ctatcgcagc tgagcgtagt cgatgcgatg
     4741 gagttcttgc aggataagat gcatggaacc aagaacaaca aagagtttct tgagtccatg
     4801 aaccagtaac tttcctcggt tttcccgcta ccttgcgcgc aacgcttatc tttgttaaaa
     4861 acgcaaagat atctaagtcg attaaacttt tttacttatc gctggcggtt gtgtgttggt
     4921 cctagaaacc cgccggcctg gatacttatt tcacaccctt tctgatccct tgcgtggtgc
     4981 cccatcgggt gcgtaacgga aaagaggaaa ggggaggaca aaaccaaagg agaagagaat
     5041 aatgagcgat atttctatga agcaattgct ggaagccggt gtccatttcg gacaccaaac
     5101 cagccgttgg aaccccaaga tgaagcctta catattcggc gctcgtaatg gcattcacat
     5161 tatcgaccta cagcagacgg tggcaatgtt caaagattta gaggctgtgg tgcgcaacat
     5221 cgcggccacg ggcggacacg tcctgttcgt cggtactaag aagcaggctc aggaggcagt
     5281 gaaggaagag gccgagcggt gtggaatgtt ccatgttcaa aatcgttggt tgggcgggac
     5341 actaaccaat ttctcaacca tccgtcagag cattgaacgt ttaagaagac tcgaggagat
     5401 ggaaaacgat ccaaaaatcg tcgacgcttt gactaagaag gaaatgttgg ggcttaagcg
     5461 cgaaaaagaa aaactagaac agtctctggg tggaattaaa agcatgcgaa agcttcccga
     5521 cgccatcttc gtcgttgatc cgaaacaaga agagattgcc gtcaaagaag cgcgcaagct
     5581 ggggatacct gtcgtcgccg ttatagatac aaattgcgat cccgatttgg tggactacaa
     5641 ggtgcccggc aatgatgatg ctattcgggc catccggctg ttttgttccg cgattgcgga
     5701 tgccatcaat gaaggaaaaa tgctttacga acagtcgctc gtcaaagaca aagcggatga
     5761 aaaggccgcc agcgacgtcc ctaccgagga gccgagtcgg gcgacagcaa gtcaggcgat
     5821 gggaggagtg tagggttcca tggaactcaa tgcaactgtc gtgaaagaat tgcgggagaa
     5881 gaccggcgcg ggctttttgg actgtaagaa ggctctcgcc gaaaccggcg gaaacctgga
     5941 aaaggccgtc gactatttgc ggcaaaaggg tttagccgca gccgcgaaga aagccgaccg
     6001 ggttgccgcc gacggagccg tgggcgccta cgtgcatccg ggaggcaaga tcggggttct
     6061 cgtggagatc aactgccaga cagactttgt tgctcgaacg accgagtttc agactttact
     6121 gaaggatatg gccatgcaga tcgcagccgc cagtccgcgt tatgtgaaac gagaagacgt
     6181 atccgttgac gagatcgaaa aggagcgaga gatctaccgc ggccaggctc tggagtctgg
     6241 caagccggaa aaggtagtcg agaagatcgt cgaaggaaaa atggagcgat tctattctga
     6301 ggtttgcctc ttggagcaag cgttcatcaa agatcctgat cgtaaggtga gtgacactat
     6361 taacgatgcc attacccgtc tgggcgagaa aattgaagtg aggcgattca ctcgctacca
     6421 tctcggcgaa ggagttcaga aggattagaa tgcccagaga gactaatgaa caacgggtcg
     6481 ccaaaatata aacggatcat cctgaaggtc accggcgaag ttttcgcggg accgcataaa
     6541 tttggtatcg acggaaagac cgtcaaggca ttcgcccaag aaatccaaga agtgaaagag
     6601 atgggttgtg agttggcatt ggtcatcggc gggggcaata tctttcgtgg agccgtggca
     6661 agcgaaatcg gcatggaccg ggccagtgca gacacaatgg gcatgctagc caccgttatc
     6721 aatagtttgg cgctgcaaga cgcgctcgag aagctgggag tttcaactcg ggtattgtcc
     6781 gccattgaaa tgcgacaggt ggcagagccc tacatacggc gccgagccac gcggcacctg
     6841 gagaagggtc gcgtggtgat attcgctggc gggactggca acccttactt tacgacagat
     6901 accacggcca gccttcgggc gatggaagtg ggtgctgagg tcattctcaa ggcaaccaag
     6961 gtcgaaggag tctatgacgc ggacccgctc aagcacgcag gggctcacaa gttcgaggaa
     7021 ttgacttaca tcgaggttct gaatcgcgag ttgaaagtca tggactccac ggcgatatca
     7081 ctctgtatgg acaatcaact gcccattatc gtcttcaata ttatggagaa gggcaatatc
     7141 aaaagagttg tttcagggga gcccattggc actctagtca gcggagggaa gcgatgaacg
     7201 aagccatttt cactcagctg caaaaagaga tggatcaatc cgtcggcgcg ctgcgcaagg
     7261 atctcgccaa aatccgtacg ggccgtgcct cgacagctct gttagagcat gtcgtggtcg
     7321 actattatgg ggcgacgaca cccctgaacc agttggctac gctgagtgcg ccggaaccgc
     7381 gtctgctggt ggtacagccc tacgaccgca gtgccatggg aaatatcgaa aaagcgattc
     7441 tcaagtcgga tcttggactt accccgaaca acgacggtaa gattatccgc gtgccgattc
     7501 cccagctcac tgaggaacgg cgcaaggaac tggtgaagca cgtcaagaaa attggtgagg
     7561 agtttcgcgt ctcggttcgt aatcatcgtc gcgtcgccgt agagcatctc aaagaagctg
     7621 aaaaaaagaa agaaatcacc gcggatgatt ccaagcatgg tcacgaccga gtgcaaaaaa
     7681 tcaccgatga cttcatcggt cggattgaca aaataatcaa gaccaaggaa gaagagatca
     7741 tggaggtttg aataaccgcc gatctccgcc tgtcacgacg ccttcaggtt gagttttgtt
     7801 gcagagcccc tgcaggcgtt cgccacacgt tccattcatg aaactgacgc tgctcggcta
     7861 acgatggatc taagcggcct agacaagagt cgtttacccc agcatgttgc cattatcatg
     7921 gacggcaacg ggcggtgggc caaggaacgt gggaaaagcc gtatcgaggg ccatcggcga
     7981 ggaaaaacct cagttcgcgt catcgtcgaa atgagccgca aaatcggcat tccgttccta
     8041 tcgttgtacg ctttttcaac agaaaactgg ctcagacctc aagacgaagt ccatgcactc
     8101 atgggacttt tggaacatta tctcgccgcc gaacaggcga aaatgatgcg ctacagcatc
     8161 cgacttgtcg cgatcggcga ccggagccgc ctgccgatca gcgttcggcg cacactcgaa
     8221 cgcgtgatag acctgacacg tgacaacaag cggatgacgg tgatcctcgc gctcagctac
     8281 agtggtcgta atgaaattgt gagaatgatg aaacgaattg cgagcgaggt gcgggagggg
     8341 acgtatgacc ccgaggagat cgacgaaaaa actattgcgg cgcacatgga cacacccgat
     8401 gttccggacc ctgacctttt aatccggacc agtggtgagt tgcggatcag caacttttat
     8461 ctttggcaga ttgcctatag cgaactctac gtgacctcta ccctttggcc cgattttcgg
     8521 gaaaaggact atctgcaggc cctcatcgag tttcagcagc ggcgccggcg atttggtaga
     8581 accgacgaac aagtcatgga tcccgcccct tcatcgttgg aggccagtgg aatcaagtct
     8641 taggtcaagg gtgcttaccg cgagcgtcgg tattccgctg ctcattgtgc tggttggctg
     8701 ggggccctct tgggcttttg ttggattttt tgtcgttctt aacgcagcag cattgcgcga
     8761 gtattttgcg atggcgttcc ccggtcgtat gagagaacag ttgtggggaa tacttttcgg
     8821 cctcagtcta tccctggcgc tgcttttttc tcaagcgtca aaaggagagg tgggcttgag
     8881 cgttctgttt gtgttttttt tctcgatcta tctattcatg gcgggaaaat tatcggaacg
     8941 attgaatcgg ctcgcgtgga cgctcctcgg tgccgtctat ctcggttatc tgctgcccca
     9001 ttgggtcttg ctgtttcgca tgcctcatgg tcgcgcgtgg gtctttttcg ttctcttggt
     9061 gataatgatg ggcgatacgg cggcttatgt gaccggcact tattttggca agcgaaagct
     9121 cgcgccggag atcagtccgg ggaaaaccat cgaaggaacc ttggggtacg tggcagggag
     9181 cgtggcgggt ggctgcctcg gagcgagttt cttggttacg gaattgggcg cgatcgaagt
     9241 cgtcgcgctt tccggaatgt tgagcatcct gggacaaatt ggcgatcttt ttgagtcttt
     9301 gattaaacgc gtttttgccg tgaaagattc gggtactgta ctgccgggtc atggcgggtt
     9361 gttggatcgg ctggatagct tgatcttccc tgcggttttc gcgacgactt acttgaaagt
     9421 gtttcatcca tgagaacgat cgctcttctt ggttccacgg gctctatcgg gttgagtacc
     9481 ctcgatctcg tccgccgatt ccccgagcgt ttcaaagttt acggattggt tgccggggaa
     9541 aatctcaagc ggctgatgtc gcagattagg gaatttgcgc ccaagtgtgt cgccatcaag
     9601 aatgaggaag acgtgccacg tctgcgcaag ctgctcggcg acaatcgagt ggaggtgctt
     9661 tgcggagaac ggggggcggc cgcagtttcc acggcccatg aggtcgatat cgtcgtggcc
     9721 gccatcgtcg gtggagccgg gctggtgccg actcttgcgg cggtacgagc gggcaaagaa
     9781 gtggccctcg cgaataaaga ggcgctggtg atggccggtg agattttcgt cagcaccgcc
     9841 aagcaaaagg gagtcagact cctgcccgtg gacagcgagc acagcgccat cttccagtgt
     9901 ctgcaaggta accgacggga cgaagttgac aaactcattc tgacggcgtc gggaggtccg
     9961 tttttgcgca ccccgctcaa gctcctgaat cgggcaaccg tcgcgcaggc gttaaagcat
    10021 ccgaactgga agatgggccg gaaaattacc atcgattcag caacgatgat gaataagggg
    10081 ctagaggtcg ttgaggcgcg ctgggaattc gatatggatc cgagtcggat cgacgtcgtt
    10141 atccatcctc agagtgtggt tcattcaatg gttcgatatc aggacggatc aattattgcg
    10201 cagctcggca tccccgatat gcgcattccc atcgcttacg cgctgactta tccgcatcgg
    10261 ttgaaggggg gctggaagcc tctggatttg acccagcacg gcgagttaag ctttttaccg
    10321 gtggactcca agcgtttccc cgcgttgcgg ttggcgtacg ctgcgttgaa agagggtgga
    10381 acgatgtctg cagtcttgaa cgcggcgaat gaggtagcgg tggcggcgtt cttggagcgc
    10441 aaaatcggct ttcgcgacat acaccgtatt attgacaaga cgatgcaagg tcatgcacca
    10501 aggcgcccta aagaagtcga tgaagtcctg gaggtagatc ggtgggcccg gcaaaccgct
    10561 tcgtcgctta tccattgaag taacggcagg agaaggggag aacggatgga gaacgtaatt
    10621 tactctattg ttgcagcgat tatcggcctc ggcgtgctga ttgttatcca tgaatttggt
    10681 cattttttgt ttgcaaaatt gtttggcgtc ggagtgctga ccttttccgt cggtttcggc
    10741 ccgaaacttt gggtgaagaa aaaaggcgag accgagtacg cgctcagtgc ttttcctctg
    10801 ggtggatacg tcaagatggt aggcgaggat cccgatgaag aagtgcagca ggcggacgtc
    10861 gagaagtcat tcgcccacaa aggactcttg cggcggatgg cgattgtggc cgccggtccg
    10921 ggatttaacc ttttgctcgc cgtgtttctt ttaatgatcg tatttatttt ctacggtgtg
    10981 ccggttttat cgacccaggt cggcggtgtt gaaccgggct cgccggcgga tcgggcagga
    11041 ataaaaaaag gcgatcgtat tgtagccgtt agcggtcaac cggttaccga gtgggaggag
    11101 ttgtctaaac ggattaagga aagccagggt agcccgttaa atctctcgat tcggagagac
    11161 gctcaagacg ttgcccttac ggtggaaccg acgaaacgag agagccgaac gatctttggt
    11221 gagaaaaaag acgaatggat gatcgggatc ggtagtcatg tctccattga aaagggaaac
    11281 ccggggttag cgattgtgaa ggccgtacag caaacctatg attatgccaa gctcaccctc
    11341 gtggccctat acaaaatgat cagcggtgag gtttcgccgc gcaatctcgg ggggccgatt
    11401 ttgattgcgc agatggcggg tcaacaggcg caggaggggt taggtagttt tctcgggttt
    11461 ttggcgattc taagcataaa tctgggagta ctgaatcttc taccgatccc cgtacttgac
    11521 ggcggacatc tgctgttttt tctggttgaa gccgtaattg gtaaaccggt tgcggttaga
    11581 catcgagagg tggcgcaaca agtgggaatt tttcttttga tgcttttgat gatctatgcg
    11641 ttttacaatg atattgccag gttttttgaa aaacaggttg ggggatgagg gttatcggta
    11701 tcgacacggc aagcggcgcg gccagtgtgg ctttggtcga aaatggattg ttactgtccg
    11761 agaaaaccga tttacgctgc gagtccgctc gcgacgcaat cagacgcggt ggcaaaagca
    11821 atcgcgcgga gaccctcctt ccgctaatcg agttgctctt cgaaagcacc ggagtctcac
    11881 ttcagaacat taccggattt gcactctcca tcggcccggg ttctttcacg ggtcttagaa
    11941 ttggattgag cacagttaaa ggtcttgcct atggttggca aattcccgtc gttggagtgt
    12001 caacattatt tgctcatgcc gcaagggtca cagattatga aggcttgatt tgtgccctcc
    12061 tagatgcacg taaaaatgag gtctatgccg cggtatttcg aaagaccgcg gacatcgtca
    12121 atcgcgtgac ggaagatgtt gttgcctccg ccgccaatgt ggttgaaatg gttcgaggtc
    12181 tccagagagg ttcaccttgc ctgttcgtcg gcgacggtgc cgtcgtctac aaacatttgt
    12241 tcctggaatt gcccggggtt tgtctgcctg aaacgacaaa ttatccaacc gtcgctgccg
    12301 ccgttgcgcg cctcggcgag gatcgttttc ggtccaacag agttgatgat ttgggcgcat
    12361 tgacgcccgc ttatgttcgt ccgtctgaag ccgagttcaa gcgcggaagt tcactctaac
    12421 ttgccgatca gccgaagata aaacctacat tgacaaaatt actaccctaa gataaattga
    12481 gtcccgctaa aaaatcaatc acacaaaaaa aggaggcccc atggaagtga aagaagaaca
    12541 ggttattgcc tctcttgtgg ataaggatcc tgagctaaaa aaatattacg aagagcacca
    12601 ggagctcgaa agaaagttag cggaatttca acacaaacat cacttatcgc ctgacgaaga
    12661 agtagaaaag aaaagactac aaaaactgaa actactcggg aaagataaaa tcatggaaat
    12721 tcttggcagg caccgccagg ccggtgcgac tcaataggga gctcgaatga aaaagaccgg
    12781 agccgatatt tttgtcgaat cactgcggca tgagggggtc aagaccgtct ttggactacc
    12841 gggcggtgtg gtcctgaaga tattcgacgt gctgtgtcag cagaaggata tcgagctggt
    12901 tctgacgcgc catgagcaag gggccgcgca catggctgaa ggatatgcca aggcaactgg
    12961 caagccgggg gtcgtgcttg tcacttcggg acccggaatg accaatatcg tcaccggttt
    13021 ggccgatgct tacatggatt cggttccgtt ggttgctttc acggggcaag tgtcgacaaa
    13081 cttgattggt aacgatgcgt ttcaggaagc ggataatgtc ggcatcagcc gtccgtgcac
    13141 gaaacataac gttctcgtga aagatgttaa tgatttggct caaaccatca aagaggcatt
    13201 ttatattgct atgagcggcc ggccggggcc ggttctggtc gatattccca aggatgtgag
    13261 caccgagaag gcggaattca agtatcccga taaagtaaac ttgcgcggct ataatccgac
    13321 gatcagcggc aataagcatc aaattaagca agcggcggaa gagatcatga aggcgaagaa
    13381 gccgatgatc tatgtgggcg gaggcgcgat tttttccgat gccgccgacg aaatccggga
    13441 gttggccgag ctcacgcaaa tcccggtgac catgaccttg atgggtctcg gcagctttcc
    13501 gggtacccac ccgcttgcgc tgggaatgct cggcatgcac ggtggttact ggacgaacat
    13561 ggcgatgcat tattcggact tgctgatcgc ggttggcgct cggttcgatg accgtgttac
    13621 cggcaagctc tccgagtttt gcccggaggc gcgggttatt catatagaca tcgatccgac
    13681 atcgatcaag aaaaccttcc acgcgcatat tcctatcgtc ggtgatgtca aagccgtgct
    13741 gcgccagatg aacgtgatgt tgcgttctat ggatgggaat gcgtcggagg tgaaggcgct
    13801 ccgcgcccca tggttgaagc agatctacga atggaagaaa ggccatccgc tgacgtacaa
    13861 gcaggatagc aagatcacca agcctcagtt tgtcatcgaa cggctttttg agttgactaa
    13921 acacaacgcg attgttgcta ccgacgtggg tcaacatcaa atgtttgccg ctcaatactt
    13981 caagggcacc aagcctcgga cttggttgac ctcgggcggg cttgggacga tgggatttgg
    14041 cttcccggcg gcgctcggcg cgcaaaaggc ttttcctgaa aagctagtgt tgtgcatcac
    14101 cagcgagggc agcttccaaa tgaatctcca ggagatggca acggccgtgg agcacaagct
    14161 gccggtcaag attgttctgc tcaataacgg cttccacggt atggttcggc agtggcaaga
    14221 tctcttctac gaaggccgtt attctgcgag ctacttggga aaacttcctg acttcgtcaa
    14281 actggcggag gcatatgaca ttttgggact gcgagcggag aagcctcgtg aagtcgagtc
    14341 ggttctcaag gaaggcttga aacacaaggg cccggcgctc atggatattc ataccgaccc
    14401 atttgagaat tgttacccca tgattcctgc tggtggcgca catcacgaaa tgatgctgga
    14461 ggatccgccg gaactgaccc aagcgaagaa ggggggatcg cacaagagaa aggtggacga
    14521 gggggagggg gttcttcctg cctgatttcg tcagcgaatg gaacacatca tatcggtcct
    14581 ggtagaaaac aaattcggtg ttttggctag agtggcgggt ctgttcagtg cgaggggcta
    14641 caatatcgag agcctttccg ttgctcccac cctagatccg acgacttcga tgatcaccat
    14701 cgttaccgcc ggcgatgacc caatcatcga acagatcatg aagcagttaa acaaggtggt
    14761 ggaagtgctt aaagtcgttg atctaaccga aactgatttc ctcgaccggg aaaccgctct
    14821 gatcaaggtt cacaccaagc cggaacaccg tgatgaagct at
//
LOCUS       DASSWZ010000075         5771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_808581, whole genome shotgun sequence.
ACCESSION   DASSWZ010000075 DASSWZ010000000
VERSION     DASSWZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808581"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFN58_01875"
     CDS             <1..351
                     /locus_tag="VFN58_01875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET8916767.1"
                     /translation="VRHAVRMSGITGIALTKLDVLTGFKKIPLCNAYRYDGKVLHDFP
                     ASSKVMQNAQPVYEEMEGWDKPLDDVRKFSDLPAQAQKYVRRLEEIVETEIILVSVGP
                     NREQTILLKNPFDA"
     gene            complement(415..1440)
                     /locus_tag="VFN58_01880"
     CDS             complement(415..1440)
                     /locus_tag="VFN58_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916768.1"
                     /translation="MSLWKSCHAVYLFILGILLLPVISWSQSGPDPKLIEGAKKEGQL
                     VYYTTMTLDQSKQTVDRFEKKYPFIKVTLFRTGGGPLLNKIFTESRGGRHDWDVVVGR
                     GEMVLPLMERKLLASYRSPEYKMIDDQLVDKEGYWTAYYVNSYVLGWNTKLVKREDVP
                     KTYDALLNPKWKGGQISLDTEAYGMFEGLKGVWGREKALGYFKTLASLEPVLKRGNTE
                     RVQLAVAGEYPLIIAYNQTLQRMTSRGAPIDWIALEPAVTQVNPAMIGAKAPHPNAAR
                     LFYDYILSKEGQEQLRGFQRIPVRKDVEPDPPRLFRGFKYVIENPEDYKDFDATVKQY
                     LEIFKLR"
     gene            1618..1923
                     /locus_tag="VFN58_01885"
     CDS             1618..1923
                     /locus_tag="VFN58_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HET8916769.1"
                     /translation="MEGETTRERLVSDLKTLVGDAEELLKATTNQAGEKVTAARQKIE
                     QSLIEGKKALADAEKALVKRSKEYADVADDYVRDNPWSAVGVAAGVGLILGLLIRRS"
     gene            1932..2120
                     /locus_tag="VFN58_01890"
     CDS             1932..2120
                     /locus_tag="VFN58_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916770.1"
                     /translation="MPEKEGSTNGERSTSFLDTCKSVFAAITSALHTRLELLITELEE
                     ERERLKQTVKGIHAICAA"
     gene            complement(2149..3825)
                     /gene="ggt"
                     /locus_tag="VFN58_01895"
     CDS             complement(2149..3825)
                     /gene="ggt"
                     /locus_tag="VFN58_01895"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET8916771.1"
                     /translation="MFFRRKLQAKFFLLVFFFVAAFPAYAQQRAAGIVVSDHPLATQA
                     GTEILDRGGNAVDAAIATGFALAVVDQAASGLGGGGFMIIYEARDKRAHALDFRETAP
                     EAAHADLYARGGKPVPSLSLTGALAVAVPGEVAGLLAAMKRFGSLPLAALMAPAIRLA
                     TEGFPLDAALRYAIERQSSNMKRFPELGRVFLVKDEVPPEGEIIRQPDLGQTLKAVVA
                     EGADVFYQGWIAQAIVDSVKRDGGVLTLEDLRSYKPIWREPLVGSYRKRTVITMPPPS
                     SGGVALIQMLNVLEGHQLTKLAPNSATYLHLVAEAMKHAFADRAQYLGDPDFVKAPIP
                     TLLSKDYAAWIRGRISPVKTHPSKYYGLGNFKAEQGGTTHFSVLDRFGNAVACTLSVN
                     TRFGSKILVPKTGIVLNNEMDDFAIHPSGNVYGLVGNDANSLQPKKRPLSSMTPTIIL
                     QGDRPELIVGGAGGPRIISATLQTILNVLDFQMPIKEAVEAARIHHQWIPDELNVEDK
                     LPPETKKSLERRGHAVRERITLGVVQAVLAKRSNITGAADSRKADRARSE"
     gene            3931..>5771
                     /locus_tag="VFN58_01900"
     CDS             3931..>5771
                     /locus_tag="VFN58_01900"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916772.1"
                     /translation="MKTILGPFHPHLENALVEEIRGQKADNPLLPVLILVPSDSLRRR
                     LKILLTREQNLSLVNLQLLTFHQLSSRLSSEVNGANLPILRDDLFLEEVLRQIIRTKQ
                     PGTEAFAGIEERAGGCAALWQTLRDLRDGMVDPSVVLEALREGHFGARTSERTSHLLA
                     LLQTLRLFCKEKGIEDHSDLEKLTTERAPLSCFLSQFAQIFYYGFYDLTQIQVDLFHS
                     IVQNYPATLFFPLLRTQPSHEGWSFAERFYQRYVQGRTGATAMTNLVEKPALQDTLPP
                     TFRLFDQVGERAYAPLPKKWRCQILNAFGIHDEVGAAAKEILRLVADDEMPFNDIGVV
                     ARSLEIYGPPIKEIFHNHHIPMTGVIEEPLVQFPLTKAVILLLNLPAKDYLRTQVIDL
                     LSSPYFQLDSSGMEKILPRPDMWDLATRELAICKGVQEWRRLQDYTTRDLILSQVSHD
                     DEPRVIRIAAAQIRGLANIFNEISIDLETLPPEASWSHYVGAWKELLKRYLGISSGNE
                     EMELENSEDLVRAEILAILDRLAGLDVVSVNTLLSDFSQTFQHWLERSTVAAPPLNAA
                     GVAVLNATAARGQSFGALFILGLNEGVFPRTIREDAFLRDRDRETLER"
BASE COUNT         1338 a         1622 c         1487 g         1324 t
ORIGIN      
        1 gtgcgccatg cggttcggat gagcggtatc accggcatcg cgttgacaaa gctcgacgtc
       61 ttaacgggtt tcaaaaaaat tccgctatgc aacgcctacc gttatgacgg taaagtgctg
      121 cacgactttc ccgccagttc aaaagtcatg cagaacgccc aacccgttta cgaggaaatg
      181 gagggctggg acaaaccgct cgatgatgtg agaaaatttt ctgacctgcc ggcgcaggct
      241 cagaagtatg tccgcaggct tgaggaaatt gtcgaaaccg agataattct cgtttcggtg
      301 ggaccgaacc gcgagcagac cattttgtta aaaaacccct tcgacgctta ggcaacagac
      361 ctcgatatga ggtaatagca gatttccctt gtcaactcgc cctggtacct tcacttagcg
      421 tagtttaaaa atctccaagt attgcttcac cgtagcatcg aaatctttat agtcctccgg
      481 gttctcgatg acgtatttaa atcctctgaa gagccgcggc ggatcgggtt ccacgtcctt
      541 gcgcaccgga attctctgga aacctcggag ctgctcttgg ccttccttcg aaaggatgta
      601 gtcgtaaaac agtcgggccg cgttgggatg gggagccttc gctccaatca tcgcgggatt
      661 cacctgagta accgccggtt cgagtgcgat ccagtcaatc ggcgcgccgc gcgaggtcat
      721 tctttgaagg gtctgattgt aagcaatgat caaaggatat tctccggcca cagccagctg
      781 cactctttcg gtgttgccac gctttaaaac cggctccaag gacgcaagtg ttttgaaata
      841 gccgagggct ttctctctgc cccaaacacc cttgagtcct tcgaacatgc cgtaggcttc
      901 ggtatcgaga gatatctgtc cacccttcca cttgggatta agcaaggcgt cgtaagtctt
      961 tgggacatcc tcgcgcttaa ccagcttggt attccaaccc agcacgtagc tgttgacgta
     1021 ataggctgtc cagtagccct ccttgtctac caattggtcg tcgatcatct tgtactccgg
     1081 cgaccggtag gacgccaaaa gctttctctc catgagcggt aagaccattt cccctctgcc
     1141 gacgaccacg tcccaatcat gcctcccgcc gcgggactcc gtgaaaatct tgtttagtag
     1201 cggcccgcct cccgtccgaa agagggtgac cttgatgaaa ggatacttct tttcaaacct
     1261 gtctaccgtt tgtttgctct gatccagggt cattgttgta tagtaaacga gttgaccctc
     1321 ttttttggct ccctcgatca gtttgggatc gggaccgctt tgagaccaag aaatcacggg
     1381 tagcagcaat attcctagga tgaacaggta aaccgcgtgg cacgacttcc agagcgacat
     1441 agggatattt cctccttacc ctccaatggg tcaaaaagtt ggacgtatgg taagggatga
     1501 aaaaggacaa atcaactttc acgagaaaat attctgaaat caactgcggt ccttgaaata
     1561 ccccaggagg gctgattaac atggttcaag gcattcgcaa gcgaaaggga ggttaccatg
     1621 gaaggtgaaa caacgagaga aagactcgtt agcgatctga agactttagt cggcgacgca
     1681 gaggaactac taaaagccac cacgaaccag gctggtgaga aagtcaccgc ggcgcgccaa
     1741 aagatcgagc aaagtttgat cgagggcaaa aaggcgctgg ccgacgcaga aaaagcccta
     1801 gtaaagagat ccaaagagta tgccgacgtc gccgacgatt atgttcgcga caatccctgg
     1861 agcgcggtgg gtgttgccgc cggtgtcggc cttatcttag gcctgctgat tcggcgcagc
     1921 taattccgcc tatgcctgaa aaagaaggca gcacgaacgg cgaacgctcg acaagctttc
     1981 tagatacttg caaatccgtg tttgcggcaa tcacttcggc tctgcacacg cggctggagc
     2041 tgcttattac ggagttggaa gaagaacggg agcggctaaa acagacagta aagggaattc
     2101 acgctatttg cgccgcttaa tcgttcagtt tcaatagctc tcagacggtt attccgaacg
     2161 cgccctgtcc gcttttcttg aatcggctgc gccggtaatg tttgaccgct tcgccaggac
     2221 cgcctgtacc acacccagag taatcctttc tctcaccgcg tggccccttc gttcgaggga
     2281 ctttttggtc tctggcggca gcttatcctc gacgttgagc tcatcgggaa tccattgatg
     2341 gtgaatacga gcggcttcga cggcttcctt gatcggcatc tggaaatcta acacgttaag
     2401 aatcgtttgt aaggtcgcgc tgataatccg cggtccaccg gctcctccga cgatcaactc
     2461 cggccgatcg ccttggagta taatcgtcgg tgtcatgctg ctcagtggcc tctttttcgg
     2521 ctgcaatgag ttggcgtcgt tgcccaccag gccgtaaacg ttgccgctcg gatggatcgc
     2581 aaaatcatcc atctcgttgt tcagcacaat cccggttttg ggcacgagga tcttggaacc
     2641 gaaacgtgtg ttaaccgaca aggtgcatgc gacggcgttg ccgaaacggt caagcacgct
     2701 aaaatgtgtc gttccacctt gctctgcctt aaaattcccc aagccataat attttgaggg
     2761 atgcgtcttg accggagaaa tcctgccgcg aatccaagcc gcataatctt tggacagcaa
     2821 ggttgggatc ggcgctttga caaaatccgg atcgccgaga tattgcgcgc ggtccgcgaa
     2881 ggcgtgcttc attgcctcag caaccaggtg aagataagtc gcggagttgg gcgcaagctt
     2941 ggtcaattga tgaccctcca gcacgttcaa catctggatc aaagccaccc cccctgaact
     3001 cggcggcggc atcgtaatca cagttcgctt gcggtagctt ccgacaagcg gctcacgcca
     3061 gatcggctta tagctcctca agtcctcaag agtaagaaca cccccgtccc tttttacgct
     3121 atcgacgatg gcttgggcga tccagccttg gtagaagaca tcagcaccct cggctacgac
     3181 cgctttcaac gtttgaccga ggtcgggttg gcgaataatt tcgccctctg ggggcacttc
     3241 atctttcacc agaaagactc gacccagctc cggaaacctt ttcatgttgg atgactggcg
     3301 ctcgatggca tagcgcaggg cggcatccag cgggaaccct tccgtcgcca gacgaatagc
     3361 gggcgccatc aaagccgcca atggcagact gccaaaccgc ttcatcgctg ccaacaagcc
     3421 cgccacttcg ccgggcacgg ccactgccaa cgcgcctgtc aaactcaatg atggcactgg
     3481 ctttccgccc ctcgcataaa ggtcggcgtg cgccgcctcc ggcgctgtct cccggaagtc
     3541 caacgcatgg gcccgtttat ctctggcctc gtaaataatc atgaagccgc cgccgccgag
     3601 accggatgcg gcctgatcca ccacagcgag ggcaaacccg gtggcaatgg cggcatcgac
     3661 agcgttgcct ccccgatcaa gaatctccgt tccagcttgc gtcgccaacg ggtggtccga
     3721 aacaacgatg ccggccgcgc gttgttgggc ataagcaggg aacgccgcga caaagaagaa
     3781 tacgaggagg aaaaatttcg cctggagctt gcgcctgaag aacataaaag aagagagctt
     3841 accttgtctt taaatcttcg aaagctcttt tgcaacttaa aaatctttgc ccggaagctt
     3901 ggttgtgtta cttttgttcc gaagactcta atgaagacga tcttaggtcc ttttcatccc
     3961 catctagaaa atgccttggt agaggagatc cgcggccaaa aagcggataa cccgctcttg
     4021 cccgtgttga ttctcgttcc ctcagattcg ctgcggcgcc ggctaaagat tcttttgacc
     4081 cgcgaacaaa acttatccct ggtcaatctt cagctgctga catttcacca gctctcttca
     4141 cgcctgtctt ccgaagtaaa tggggcgaat ctaccgattc ttcgcgacga cctctttctc
     4201 gaagaggtat tgcgtcaaat cattcggaca aagcaacctg gcaccgaggc cttcgccggc
     4261 attgaagaaa gagccggtgg atgcgccgcg ctctggcaaa ccctgcggga cttgcgggat
     4321 ggaatggtgg acccgtcggt tgtcttggaa gcacttcgtg agggtcattt cggcgcgaga
     4381 accagcgaaa gaacatccca tctcctagcc ttgctccaga cgttgcggct tttctgcaaa
     4441 gagaaaggca ttgaagacca ttccgactta gagaaattga cgacggaaag agctcccttg
     4501 tcgtgctttc tcagtcagtt cgcgcagatt ttctattacg gcttttacga tctgactcaa
     4561 atacaggtgg atctctttca ttccatcgta cagaattatc cggcaactct tttcttcccg
     4621 ctcctgcgga cgcagccgag ccacgagggc tggagtttcg ccgagcgctt ttaccaacgc
     4681 tacgttcagg gccgaaccgg cgctacagcc atgacgaatc tcgtcgagaa acctgcactc
     4741 caagacactt tgcctcccac tttccggctc tttgaccaag tcggcgaaag agcttatgcg
     4801 ccgttaccga aaaaatggcg ttgccaaatc ctaaacgcct ttggcatcca tgatgaagtc
     4861 ggggctgccg ccaaggagat tctgcgcctg gtggccgatg acgagatgcc tttcaatgac
     4921 atcggcgtcg tggcccgtag cctcgagatc tacgggccac ccatcaaaga aatctttcac
     4981 aatcaccaca tccccatgac cggcgtgatc gaagagccac tggtgcagtt tcccctgacc
     5041 aaggcagtga tcctgctgct caatctgccg gcaaaagatt atctccggac ccaggtcatc
     5101 gatctgctgt cttcccccta ctttcaactc gactcctcag gcatggaaaa gatcctacct
     5161 cgccccgaca tgtgggatct agccacccgc gagctggcaa tctgcaaagg cgtccaagag
     5221 tggcgccgct tgcaagacta cacgacacgg gatctgattc tctcccaagt ttctcacgac
     5281 gatgagccgc gcgtgattag aatcgccgcc gcacagattc ggggcttggc gaatattttt
     5341 aacgagatct cgattgattt agaaaccctg ccgcccgagg cttcttggag tcattacgtt
     5401 ggcgcgtgga aagagctgct caagagatac ctcggaattt cgtcgggcaa tgaagaaatg
     5461 gaattagaga actcagagga tctcgtccgc gcggagattc tcgccattct cgaccgcctg
     5521 gctggactcg acgtggtgag cgtgaacacg ttattgagcg attttagcca gacctttcag
     5581 cattggctag agcgctcgac cgtagcagcg cccccgctaa acgccgctgg agtggccgtc
     5641 ctcaacgcca ccgccgcccg cggccaatcc ttcggcgcgc tgtttatcct gggccttaac
     5701 gagggagttt ttcctcggac tattagggaa gatgcctttc ttcgcgaccg agatcgagaa
     5761 accttggagc g
//
LOCUS       DASSWZ010000076         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_638807, whole genome shotgun sequence.
ACCESSION   DASSWZ010000076 DASSWZ010000000
VERSION     DASSWZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638807"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1136)
                     /locus_tag="VFN58_01905"
     CDS             complement(105..1136)
                     /locus_tag="VFN58_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8916773.1"
                     /translation="MKGIHFWLLILIFSSCPVAAQTPYYQGKTIRMMVGYPAGSAHDM
                     WGRLVAPQLTKHIPGNPATVVENRTGAGSMVAANYIYNVAKPDGLSVGVINAALYFDQ
                     LMKRPEVQFDWSKYSWVGSSTPTNALLYMWANAPYKTIHDVRSATVPPKCGVTGTGNT
                     GYYFPKLLEQAIGTKFQLVTGYQGGADIEIAVERGEVQCRAFSVQVFFGREPFNTWRS
                     KNLVRVLVQTGKKRDARLPETPLFNELMDQYQTAESYRRLAKVLFGSGEFGSAPMIAS
                     PGIPPEQVKILREAYAKGLNDPDLVAEAKKKGLDPELIHGNDMENLAKEVMVQPTEVI
                     ALMKKVMGE"
     gene            1345..2385
                     /locus_tag="VFN58_01910"
     CDS             1345..2385
                     /locus_tag="VFN58_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916774.1"
                     /translation="MQMFRVLIMVSALVISFELTPVAFAQQTPSSVENAKKEGEVVWY
                     GTLTGGSIVGRILKSFEDKYPSIKVKYLRLGGAGLIERIRSEARSGKYLWDVVTAEYI
                     QFFELPKYVTLAKYVPPEFAQYSPKHRDPNGTWVSFYGAIATIAWNTRLVKPVEAPKD
                     WKDLLDLRWKGKKIGLPAEAFQWYGGMVAYMGDKAGREYMRALAEQDPQTQAGYTNTA
                     NLLVAGEFPVAVIRAHRIEDARSKGAPVDWSAEANPIVISIHPIGISEKAAHPNAARL
                     FEDFLTSVEGQSLFTEEGFLSSHSGVKPRFPRMSLDRIKYPAPPDTKVSERIQTWITE
                     HQQTFRIPVGRK"
     gene            complement(2402..>2607)
                     /locus_tag="VFN58_01915"
     CDS             complement(2402..>2607)
                     /locus_tag="VFN58_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916775.1"
                     /translation="GSGWRLLKIVRLLEQDLQVPVIYALACKLWDVQKQFHVREPVKG
                     YGQLLEELPVNKKVSNFGFRASS"
BASE COUNT          612 a          695 c          720 g          580 t
ORIGIN      
        1 ttcgcaggca gaaatcgaca taaagcctcg aaggactcat gagcaagaca gtccgaggac
       61 aaagtcagag ccgtgtgttg cccgctagag ctctaaaagg gagtctactc gcccataacc
      121 tttttcatca gagcgatcac ctcggtgggc tggaccatga cttctttggc taagttctcc
      181 atatcgttac catggattag ctcgggatcg agtccttttt tcttcgcttc cgcaacgagg
      241 tcggggtcgt tcaaaccctt ggcgtaggcc tcgcggagaa tcttcacctg ctccggagga
      301 attccggggg acgcgatcat cggcgcgcta ccgaattcgc cggagccgaa caagaccttt
      361 gcgagacggc ggtaagactc ggcggtttga tactgatcca tgagctcgtt aaagagcggt
      421 gtttccggca gccgcgcatc gcgcttcttg ccggtttgca ccagcacacg gaccagattc
      481 ttgctgcgcc aggtattgaa cggttcccgc ccaaaaaaga cctggaccga aaaagcgcgg
      541 cattgcactt ctcctctctc cacggcgatt tcaatgtcgg cgcccccctg ataccccgtc
      601 acgagttgaa attttgttcc aatcgcttgt tccaggagtt tgggaaagta atagccggta
      661 ttgccggtgc cggtgacacc gcattttgga ggcacggtgg cgctgcggac gtcgtgaatg
      721 gtcttatagg gggcattggc ccacatgtac aggagagcat tggtcggggt cgaactgccc
      781 acccagctgt atttggacca atcgaactgc acctctggac gcttcatcag ctgatcgaaa
      841 tataacgccg cgttgattac tccaacgctt aaaccatcag gcttggcgac gttgtagata
      901 taattcgctg ctaccatgga gccggcgccg gtcctattct cgaccactgt ggcgggattt
      961 cccggaatat gtttggtcaa ctggggagcc accagcctgc cccacatgtc atgggcgctg
     1021 cctgccggat atcccaccat cattctgatg gtcttgccct ggtagtaagg agtttgcgct
     1081 gcaacggggc aacttgaaaa gataagaatc aacagccaaa aatgaatccc tttcatcgtg
     1141 aaacctcctc ttgattacca agggaaagaa gtcccggggc gtgatttcac gtaacatagg
     1201 gcgatttgtt gagcggcgtc aagtagagaa aaattgtcgt gtgtttacat cggccgccgc
     1261 agtcggatat agctgagctt ccagcacccg gatccccaga cccagccaga gtcagatgtc
     1321 tcaaaggagt ctgaaggagc cttcatgcaa atgtttagag ttttaataat ggtatcggct
     1381 ctggttatct cctttgagct gacaccggtc gcgtttgccc agcaaacccc ttcatccgtt
     1441 gagaatgcaa agaaagaagg ggaagtcgtc tggtacggca cgctgaccgg gggatccatt
     1501 gtcgggcgga ttctgaagag cttcgaggac aaatatcctt ccattaaagt gaagtatctt
     1561 cggctcggtg gagccggcct tattgaaaga atacgcagcg aggcgcgcag tgggaaatac
     1621 ttatgggatg ttgtgactgc ggagtacatc caattctttg agctgccaaa atacgtcacg
     1681 ctcgcgaagt acgtcccacc agaatttgct cagtactcac caaaacatcg ggaccccaac
     1741 ggtacctggg taagctttta tggagcgatc gcgaccatcg cttggaatac ccggctggtg
     1801 aagccggtcg aggcgcccaa ggactggaag gatcttctag atctcagatg gaaagggaaa
     1861 aagatcggtc tgcctgccga agcgtttcaa tggtacgggg gcatggtcgc atacatggga
     1921 gacaaggcgg ggcgggaata catgagggcc ctcgcggagc aggatcccca aacgcaagcg
     1981 ggttacacca acaccgccaa tctcttggtg gccggtgagt ttcccgtggc ggttattcga
     2041 gcgcatcgga tagaggacgc gcggtcgaag ggagcaccgg tggattggtc cgcagaggcc
     2101 aatcccattg ttatcagcat tcatccgatc ggtatctccg aaaaagcggc gcatccaaat
     2161 gcggcgagac tcttcgaaga tttcctcacg tcggtggagg gccagagcct tttcactgaa
     2221 gagggtttct tgagctctca cagcggcgtc aagccgcgct ttccgagaat gagcctggat
     2281 agaatcaaat accccgcccc acccgatacc aaagtaagcg agcggatcca gacctggatc
     2341 accgaacatc agcagacgtt ccgcatcccg gtgggccgaa aatgacaatt gctttttctg
     2401 atcaactcga cgcgcggaac ccgaaattcg aaactttctt gtttaccggc aattcttcga
     2461 gaagctgacc gtatcccttg actggttcgc gcacgtggaa ttgtttttgg acgtcccaaa
     2521 gcttgcacgc cagtgcataa atcaccggca cctggagatc ttgttccagg agccgaacga
     2581 tcttcagcag gcgccagccg gagccga
//
LOCUS       DASSWZ010000077         9600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_667037, whole genome shotgun sequence.
ACCESSION   DASSWZ010000077 DASSWZ010000000
VERSION     DASSWZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667037"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..980
                     /locus_tag="VFN58_01920"
     CDS             213..980
                     /locus_tag="VFN58_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8916776.1"
                     /translation="MKNSQTGARYFVAAGALGSLLLMQGCLATRDWVKEQMDPVSSRV
                     ATNEQKLTQVESQVTSLSKSTTDKFGQVEGRLGQFEGRLGQVDAKTDKALNTLANLRL
                     ERRFVIDMKEGANFAFNSANLPAQAQKEIDGFLSDLKGDAAGADGTIFLVAGHTDNVG
                     SEDINFELGKKRADAVSRYLITQKKLDPLKVISVSYGESAPLQDNSTGQGRAKNRRVE
                     ILVYRDGITSAPSAAAAPSSQAEPRASQQGGERLSQR"
     gene            complement(1039..1971)
                     /locus_tag="VFN58_01925"
     CDS             complement(1039..1971)
                     /locus_tag="VFN58_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916777.1"
                     /translation="MALEEFRIIYRSNSHAPLWVVAEKSGVWEKNGLAVNTSPQPVRE
                     KAAEALRNGRVDLISGNHHNLYVRNARGEDFVHLAQAGNNWTENRLVVAHGIGSVMDL
                     KGKKVVVDKLTSHAGLNVWLFLRQEGLDADRGDVDLVELRGPAEERWKRVLNGEFAGT
                     FVTLPHDTRAKRAGAQVIRVRAIPMIRGVTLTTTMSFVKTHEAKVRLLIRGFVDAIHF
                     FITRKQETLEILKEHASPILHLESGEEVETLYEEWAHSLERKPYPSIEAIANVFRLAV
                     RRNPEIASFNPLALWNTHYVRELDDSGYIDQLYQ"
     gene            complement(2044..2517)
                     /locus_tag="VFN58_01930"
     CDS             complement(2044..2517)
                     /locus_tag="VFN58_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916778.1"
                     /translation="MLNSWLHLIALAVYVGAVVGLWAMLLPAASKIENHKDKVPFLVR
                     SLKLYSPIQIGALGVLLFTGAFQLTDLKAVYRELFVKQLGYNLGIKLFVAFFLVLFSV
                     YQSMGIAHRFVKRHEAGDAITSQEFDSIIRKLKSSNGLILILALITLWFGLRLRS"
     gene            complement(2530..3366)
                     /locus_tag="VFN58_01935"
     CDS             complement(2530..3366)
                     /locus_tag="VFN58_01935"
                     /inference="COORDINATES: protein motif:HMM:NF033683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HET8916779.1"
                     /translation="MATMITNDCINCGACEPECPNNAISQGETIFIIDPMLCTECVGF
                     HDYEACAAVCPVDCCVTDPNNIETEEALIARARGLHPDTRFDDNFESRFRKGQGQPAA
                     AGAKTDAPARAASAANNSATEVTSATQQASAGTSSAYAEPGVNFVTLPDIDSWDIPVR
                     CFKCKQPHVEPVRNFMIGNVIFCPHCNKSMVVRDNLNFHVRTLLRDSYDKWQNEQNEF
                     QGKRQRELTEFVEDRAREAAAFEAYQQRELQKIRQQLDAMGETYDAPGKPIKKGSRFG
                     WG"
     gene            3581..4621
                     /locus_tag="VFN58_01940"
     CDS             3581..4621
                     /locus_tag="VFN58_01940"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916780.1"
                     /translation="MGYKRIMKMSQSTVLLNAIAFLVALGLILSPNLSPAADKSKKLR
                     LAYSGWEIGTAVSYVGVDSGLFKKHDVDIEELPIRDTLSAGVQSLLGVDLLIGFGNPL
                     AILQPVAGGADITLIGSHVSFDNYGMGVASSISAIKDLKGKKVGVSALGTRSDLIARV
                     ILRRAGLDPAKDVEVVAAGLSPSRALAISKNLVQGAPLSPEVAAEAQKLGIKILEVKP
                     VPVVTDLIMTTRSFIRKEEDTVRRFMKGYAAAIHHFVTKRDDSLAILKKYFPGNQSVT
                     VDAMYDSFAAQLRPLPELTVEAIQALVDVGTVADQRAKSLKPTDIIEPRFLEELKGSK
                     FLKDLYTEKVSL"
     gene            4777..5523
                     /locus_tag="VFN58_01945"
     CDS             4777..5523
                     /locus_tag="VFN58_01945"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8916781.1"
                     /translation="MRLKDQVAIVTGAGRNIGEDICKLFVQEGAKVAVVDLDPGRGGR
                     VAGEINAAQPGKAFAIVCDVSSADSVEKMVQRVTQEFGGVDILVNNAAWTDHKTVFDI
                     TDEEWDRTMNVCLRSVWYCTRSAARAMIDKKKRGKIINIASTSGHWGRKDATAYTTAK
                     AGVLNLTRSMAVQLASEGIRVNSISPNRIGSPVGQEAVPENRVVRNLAGRRGVPMDIA
                     KAAVFLASDESDFIYAVDLPVDGGALAVRD"
     gene            complement(5644..6117)
                     /locus_tag="VFN58_01950"
     CDS             complement(5644..6117)
                     /locus_tag="VFN58_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8916782.1"
                     /translation="MNLADQITTQGPEPTILVVDDNKGVLEFLLLLLSKHGLSVIGAS
                     SGNQCLNIVKTRPVSLIILDVMMPAMDGLEVCQELKKTIPSIPIILLTARDDMATRAA
                     AMDLGVSEFVAKPVNNRDLLDRVRVQLRNLEWDKTADQAFWRIEKSTRGAGPKKP"
     gene            complement(6143..7186)
                     /locus_tag="VFN58_01955"
     CDS             complement(6143..7186)
                     /locus_tag="VFN58_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8916783.1"
                     /translation="MLSSSSAMLGEHPSIKKIYEIIQRVAATDVTVLITGESGTGKEL
                     VARAIHNVSLRADRPFVPVNCAAIPENLLESELFGHARGAFTGAHSSRAGLFQLANHG
                     TILLDEVGELPLPLQAKLLRVLQDGKVRPIGTDHHVEVQVRVIASTNKDLAHQVEKGS
                     FREDLFFRLQVIPLHLPPLRARRSDIPLLVNHFLDKSTRKHGVSVSLTREAMIYLWEY
                     DWPGNIRELENLIERLVLLSDNGITDLKDLPPNIRSYVSDKKCPHPTLGSGQIDLRDA
                     TEQLQYRLMEEALRLTNGNKSAAARMLGLKRTTLVAKLRRKPRPNGGSFTLASEPEEP
                     LSVFLAASGVKDR"
     gene            7587..8747
                     /locus_tag="VFN58_01960"
     CDS             7587..8747
                     /locus_tag="VFN58_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8916784.1"
                     /translation="MQLEITPQSEASQFNNALSNPLVGNSARMHIVRALIARLANSNS
                     TVLITGESGTGKELAARAIHELSPRQNGCFVPVNCGAIPEELLESELFGHVRGAFTGA
                     VNARQGRFQFAHGGTLFLDEISEMSAKLQVKLLRVLQERQFEPVGSDSPVQVDVRVVA
                     ATNRDLRVAVRERRFREDLFYRLNVLPLELPPLRERDADLVLLIRHFLTLHARRKGKT
                     AIQVNEDAMAIMKRYSWPGNVREVENLVERLVVLNEDGIVRPTELPDYVIHNAMPQHQ
                     TTAQVFLPSDGVDLDGFLDKIEDGFIQQALERSRGNKTLAAELLNLNRTTFIERLRKK
                     GMLQSTRRASLPSTVVNNVPEDGTPLTEPKFKQPSPNGLSPADSVKVGSLSF"
     gene            8812..9021
                     /locus_tag="VFN58_01965"
     CDS             8812..9021
                     /locus_tag="VFN58_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916785.1"
                     /translation="MNFKLQMPALNQSEENVRVLKGLISRFNQSSLELIREYRRLEER
                     AGYLKGQRESVREPCRRGQKNFHGH"
     gene            complement(9407..>9600)
                     /locus_tag="VFN58_01970"
     CDS             complement(9407..>9600)
                     /locus_tag="VFN58_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916786.1"
                     /translation="LTVDYDNFGTESISKNRFCMEVNTIVDGTKRQGNFTFRPDRALP
                     AGGQNPGFFVFILPVANAR"
BASE COUNT         2292 a         2379 c         2615 g         2314 t
ORIGIN      
        1 ctcagaattt tggcgcgact gttctcagga aaccttttgg agaaatggag ttacttaaaa
       61 taattcaatc acttacaaga tcaggctcag agaattttga atggtcgtct gatcaaagga
      121 aatatggttg gaaagaagca atttaactga aggattttcc ttcagttaaa ctaattataa
      181 aggaacggat caataaagga ggagaatggt ttatgaagaa ctcccaaacg ggggctaggt
      241 atttcgtcgc agcgggcgca ttagggtcgt tgctgctgat gcaaggttgt ctcgcgacgc
      301 gtgactgggt gaaagagcaa atggatcctg tatccagtcg agtggcaacg aatgagcaaa
      361 agttgactca agtcgagagt caagttacaa gtctcagcaa aagcacgacc gacaaattcg
      421 gtcaggtgga aggtcggctg ggacagttcg aaggccgctt gggtcaggtc gatgcgaaaa
      481 ccgacaaggc tctaaatacg ctggctaact tgaggttgga gcggcgtttt gtcatcgata
      541 tgaaagaggg agccaatttt gcttttaatt ccgcaaatct gccggctcag gcgcagaaag
      601 agatcgacgg gtttctcagc gatctcaaag gagacgctgc cggggcggac ggcacgattt
      661 ttctcgttgc tggacacacg gacaacgtcg gttccgagga cattaacttt gaactcggca
      721 agaaaagagc cgatgcagtg agccgctatt tgatcactca gaagaaactg gacccgctaa
      781 aggtgatttc agtttcctat ggtgaaagcg cccctctaca agacaacagc acgggccaag
      841 gccgtgctaa aaaccgtcgg gtcgaaattc tagtttaccg cgatggtatt acctcggcgc
      901 cgagcgccgc tgcagccccg tcatcacaag ccgagccgcg cgcgtctcag cagggtggag
      961 agagactgag tcaacgatag acgcataaaa ggtcctggca atgtagacaa aaaggcgggg
     1021 ttttaacccc gccttttttt attggtagag ttgatcgata tagccgctat cgtccagttc
     1081 gcgaacgtag tgagtattcc atagcgccaa tgggttaaag ctcgcgatct ccgggttacg
     1141 cctgaccgca agccggaaaa cattggcgat ggcttcgatg gaagggtagg gtttccgctc
     1201 caatgaatgg gcccattcct catagagcgt ctctacttcc tcacccgact ctagatgcag
     1261 aatcgggctt gcatgctctt tgagaatctc caaagtttct tgcttgcgcg tgataaagaa
     1321 atggattgca tccacaaaac ctcgaatcag aagccggacc ttcgcttcgt gggtttttac
     1381 gaagctcatg gttgtggtca gtgtcacgcc gcgtatcatg ggtatggcgc gcacgcgaat
     1441 cacctgggct ccggctcttt tcgcgcgagt gtcgtgagga agagtgacga acgttcctgc
     1501 gaattcgcca ttcaacacgc gcttccaccg ctcctccgcc ggtccacgca attccacaag
     1561 gtcgacgtcg cctcgatccg cgtcgagtcc ctcctgacgt aagaaaagcc agacattcag
     1621 cccggcgtga gaggttagct tgtcgaccac tacttttttg cctttgagat ccatgactga
     1681 accaatgcca tgagccacca ccagccggtt ttccgtccaa ttgttacctg cttgagccaa
     1741 gtggacgaaa tcctcacccc tcgcgtttct cacgtagagg ttatgatggt ttccggagat
     1801 caaatcgaca cggccattcc ttagagcttc cgccgctttc tcgcgtaccg gttgaggcga
     1861 cgtattaacc gcgagaccgt tcttctccca gaccccggat ttttccgcca cgacccagag
     1921 cggtgcgtgg gagtttgagc gataaatgat tctgaattct tcgagagcca tcggagttct
     1981 cctactataa tacgtttcgt ttacagggac ccagggagga gatgctgtac tcgcgggttg
     2041 agactaggag cggagtcgta gtccgaacca taatgtaatc agggcaagaa tcagaatgag
     2101 cccattcgag ctcttgagtt ttcggatgat cgagtcgaat tcctgagatg tgatggcatc
     2161 tccagcctca tgtcgtttca cgaatcgatg cgctatcccc atggactgat agacactaaa
     2221 cagaacgagg aagaatgcaa cgaatagctt tatgcccaag ttatagccca gctgcttgac
     2281 aaacagttcg cgatacacag ccttgaggtc cgtgagttgg aaagcgccgg tgaaaagaag
     2341 gacgccgagg gcaccgattt gtatagggct gtaaagcttg aggctgcgga cgagaaatgg
     2401 gactttgtcc ttatgattct cgatcttcga cgctgccggt aacaacattg cccagagacc
     2461 gacaaccgct cccacgtata cagcaagggc tatcaggtga agccaggagt tgagcatgag
     2521 agaaaaggtt cagccccatc cgaagcgtga tcctttttta atgggtttgc ccggtgcatc
     2581 ataagtctcg cccatggcgt cgagttgctg acgaattttt tgcagttcgc gttgctggta
     2641 agcttcgaag gccgccgctt cccgggcgcg gtcctcgaca aactctgtaa gctctcgttg
     2701 gcgtttccct tgaaattcgt tttgttcgtt ttgccatttg tcgtaagaat ccctcagtag
     2761 ggttcggaca tgaaaattca aattgtcacg caccaccatg gacttgttgc aatgtggaca
     2821 gaaaatgacg ttgccgatca tgaagttgcg caccggttcg acatggggct gcttgcactt
     2881 gaagcagcgc acgggaatgt cccacgaatc aatgtctggt agcgtgacaa aattaactcc
     2941 gggctccgca tacgccgagg aagtccctgc gcttgcttgc tgggttgcgc ttgtgacttc
     3001 ggttgctgag ttattggccg cagatgcggc tctagctggc gcatctgtct tcgctcctgc
     3061 ggccgccggc tggccttggc cttttcggaa tcgggactca aaattgtcgt cgaatcgtgt
     3121 atccggatgt aacccccgag cacgcgcaat cagtgcctct tccgtctcga tattgttcgg
     3181 atcagttacg cagcagtcca ccggacaaac cgcggcacat gcctcgtaat cgtgaaaacc
     3241 gacgcattcc gtgcatagca ttggatcgat gataaagatc gtttcccctt gcgagatcgc
     3301 attgttggga cactcaggtt cacaggcgcc gcaatttatg caatcgttgg taatcatcgt
     3361 tgccatcgac tcgatcctcc taacgcttgt tggaattggt accggatgaa gactaaataa
     3421 tcctaaatga aattctggga ggttgcaaat cggcggccgg ctggagcgcg acggcccaaa
     3481 aaattccaat aacatagggt acgcgcatca gccccgagtc atgctgttcg caagggaggc
     3541 cgaattctcg tgctattttg cccttggcgt ggaggggaat gtgggttata aaagaattat
     3601 gaaaatgtca cagtcgacgg ttttgcttaa cgcgatcgcc tttttagttg ccctcggact
     3661 catattgagt cccaatctct cccctgccgc ggataaatca aaaaaactaa ggttggccta
     3721 ctcgggctgg gaaattggta ccgcggtgtc ctatgtggga gtggattccg gtcttttcaa
     3781 aaagcacgac gtggatattg aagagctccc gatccgggac acgctctcag caggtgtcca
     3841 gtctttgctc ggagtcgatc ttcttatcgg ctttggtaat ccgctggcga tcctccagcc
     3901 cgttgccggc ggcgcagata tcaccttgat cggttcccat gtcagcttcg acaattatgg
     3961 tatgggtgtg gcttcgtcga tttcagccat taaagactta aaaggtaaaa aagttggcgt
     4021 ttccgccttg ggtacgcgat ccgacctgat cgcccgcgtc attttgagac gcgcgggcct
     4081 ggaccctgcc aaagatgttg aagtcgttgc agccggcctt tcgccatcca gggcactggc
     4141 aatttcgaag aacctggttc aaggcgcgcc tttgagcccc gaagtcgctg ctgaggcaca
     4201 aaaactgggc attaagatat tggaagtaaa gccagtcccc gtggtaaccg atctcatcat
     4261 gacgacccgc tcgttcatca ggaaagaaga agacaccgta cggcgcttca tgaagggtta
     4321 cgcagccgca atccatcatt ttgtaactaa acgtgacgac agtctcgcaa tcctcaagaa
     4381 gtattttcct ggcaatcagt cggtcaccgt agatgccatg tacgatagct ttgcagccca
     4441 gctgcgaccg cttccggagc tgacggttga agcgatccag gctctcgtgg acgtcggaac
     4501 agtcgcggat cagcgcgcga aatcgcttaa gcccaccgat atcattgagc cacgcttctt
     4561 ggaagagctc aaaggaagca aatttcttaa ggacctttac accgaaaagg tcagcttgta
     4621 actcaggcgg tcaggtctcc ttttgtagct cccatgagcc atcagtgctg cacgcatgcg
     4681 cgatgcacct ggggtctgct gtgatcggtg tgtgtgcgtg tggaacttta ggctggctgt
     4741 tgttattcag ccaaaaaaat tactatggag gaagatatgc ggctcaaaga tcaagttgcg
     4801 attgtcactg gcgcagggcg caatattgga gaggatattt gcaaactttt tgtccaagaa
     4861 ggagccaaag ttgcggtggt cgatctagac cctggcagag gcggccgcgt tgcgggcgag
     4921 atcaacgccg ctcagccggg aaaggcattt gccattgttt gcgatgtttc ctcggcggat
     4981 tcggtggaga agatggtcca gcgggtaacg caagagtttg ggggcgttga tatcttagtg
     5041 aacaatgcag cctggacgga tcacaagacg gtctttgata ttaccgacga agagtgggat
     5101 cggacgatga atgtgtgtct tcgaagcgtc tggtactgca ctcgatctgc agcgcgggcc
     5161 atgatcgata aaaagaaaag aggcaagatc attaacattg cgtccacttc cggccattgg
     5221 ggtcgcaagg acgcaactgc gtacacgaca gccaaagcgg gagttttgaa tctgactcgc
     5281 tctatggcag ttcagctggc atccgagggt atccgtgtca actcaatctc tcccaaccgc
     5341 atcggttcgc cggtcggcca ggaagcagtc ccagaaaatc gtgtcgtcag aaacctcgcc
     5401 gggcgacggg gagtcccaat ggacatcgcg aaggcggcgg tttttctggc atcggatgag
     5461 tccgacttta tatacgctgt cgatttgcca gtggatggcg gtgctctcgc ggtgcgcgat
     5521 taagttcgaa tttgaacgaa tctgtcgcgt ggcattcgcg aaatcgtcgg cagcttccat
     5581 aactggcgcc tgaagcggca gaccaaggtc tgctccgaag ggttgagatt gttgagagga
     5641 tccctatggc ttcttaggac cggcacctcg cgtggatttt tcgattctcc agaatgcttg
     5701 gtccgcagtc ttatcccatt ctaaatttcg tagttgcacc cgaacccgat cgagtaagtc
     5761 ccggttgttg acgggtttgg cgacaaattc gctgaccccg agatccatgg ccgcagcccg
     5821 ggtcgccata tcatctctcg ctgtgagcaa aataattggt atcgaaggaa tagtcttttt
     5881 gagctcctgg caaacctcaa gtccgtccat cgcgggcatc atcacatcca agatgataag
     5941 gctaacgggc cgggtcttga cgatattcag gcattggtta ccgctcgacg cgccgatcac
     6001 tgacagtcca tgtttggaaa gtagcaacag aaggaactcc agaactccct tgttgtcgtc
     6061 gacgactaga attgtcggtt ccggtccctg cgtcgttatc tgatcagcca agttcattgt
     6121 tttaccgagc gatcccgaca atctacctgt ccttaacgcc ggaagcggcc aggaacaccg
     6181 ataagggctc ttccggctct gaagctagcg tgaaagatcc gccgttgggt ctcggttttc
     6241 gtcggagttt cgcgaccagt gtcgtgcgct tcaatcctag catcctggcg gcggcgctct
     6301 tgttgccgtt ggtgaggcgc aatgcttctt ccatcagtcg atattgaagt tgctctgtcg
     6361 cgtcgcgcag atcaatctga ccgcttccca gggtgggatg aggacatttc ttatccgaaa
     6421 cataagagcg aatatttggc ggcaaatcct tgaggtcggt gatgccatta tcacttaaga
     6481 ggactagacg ctcgatgaga ttctccaact cccggatatt tccaggccag tcgtactccc
     6541 aaagataaat cattgcttct ctggtgaggc tcactgagac gccatgtttt cgggttgact
     6601 tatcgaggaa gtgattcacg agaagcggaa tatcggaacg acgagcccga agcggtggca
     6661 gatggagcgg aataacctgc agccgaaaaa aaagatcctc gcgaaaggaa cctttttcca
     6721 cttggtgagc aagatccttg ttggtcgacg caataactcg gacctgaacc tcaacatgat
     6781 gatccgtgcc gatgggtctt acctttccgt cttgcaatac gcgcagaagc tttgcttgca
     6841 atggaagcgg cagctcgccg acctcgtcaa gcaatatcgt accgtggtta gcgagttgga
     6901 agagcccggc acgggaagag tgcgcgcccg taaaagcacc tcgcgcgtgg ccgaatagtt
     6961 cgctctcgag caggttttcc ggaatagcgg cgcaatttac cggaacaaac gggcgatcag
     7021 cccggaggct aacgttgtga atcgcccgcg ccactagttc cttgccagtg ccgctttcgc
     7081 ctgtaatgag gacggttacg tcagtggctg ccacccgttg aataatttca taaatcttct
     7141 tgatcgaagg gtgctcaccg agcatggccg aagaagagga cagcatggga aatttatgtc
     7201 tatgacctcc ttaagccttc gtttacagag cgcggaactt ggatcatttc ggggatctgg
     7261 cgaggaggga tggtttgact ctgggcacac ctaaattgtt caaaatcagt ctatttgaga
     7321 agttaagttt aagttaagtg tggagttttt gcaatcacgc tttgagttca cgatatattg
     7381 cccgccaatt ttctaacagc tgacattgga gtgttaattt ttcgccagca agaatattgt
     7441 tccctctacc actatctttt cgtaagattc ttcgttcttt ccgtggttta gacttgacca
     7501 agcagatcga aaacgatgtg gcattgggtt tgctcgaaga gactacacgt tcaaataatt
     7561 gtcgtcccat cggaaaagga gaaacgatgc agctcgagat caccccacag tcagaagcat
     7621 cccaattcaa caatgcactg agcaatcctt tagtcgggaa tagcgcgagg atgcacatcg
     7681 tacgggcact gatcgccaga ttggcgaata gtaactctac ggttttaatc actggggaaa
     7741 gcggcactgg aaaggagctg gcggcgcgcg caatccatga gctgagtcca aggcaaaatg
     7801 gttgctttgt gccagtcaat tgtggcgcga tcccagagga acttttggaa agcgaactgt
     7861 ttggtcatgt acgtggcgct tttacgggcg ccgtaaatgc gcgccaaggt cgtttccaat
     7921 tcgcccacgg cggaacactt tttctcgatg aaatctccga aatgagcgca aaactgcagg
     7981 tgaagttgct gcgggtactt caagagcggc agtttgaacc cgtcggcagt gatagccccg
     8041 tgcaagtcga tgtccgagtc gtcgcggcga ccaataggga tcttcgggtt gcggtgcggg
     8101 agagaagatt tcgggaggat ctcttttatc gattgaatgt tcttcccttg gaacttccac
     8161 cgctgcgcga gcgcgatgcg gacctagtgc ttctcattcg tcacttcctg acccttcacg
     8221 cgcgtaggaa aggaaaaacg gccattcaag tgaatgaaga tgctatggcc ataatgaagc
     8281 gatatagctg gccaggaaac gtcagagaag tggagaatct tgttgaacgt ctggtggtgc
     8341 tgaacgaaga cggcatcgtt cggcctacag agcttcccga ctacgtcatc cacaatgcta
     8401 tgccgcaaca ccaaacgacg gcgcaggttt ttttgcccag cgatggagtt gatctcgatg
     8461 ggttcctgga taagatagaa gacggcttca tacagcaggc ccttgagcgt tcgcgaggaa
     8521 acaaaacatt agcagcggag ctactcaatc taaatcgtac gacctttatc gagcgcctgc
     8581 gcaaaaaagg gatgctccaa tccacgcgcc gcgcttcttt gccttcgaca gttgtcaata
     8641 acgtgccgga agatggcact cctctgacgg agccgaagtt caagcaaccg agcccgaacg
     8701 ggctttcgcc tgcggactcg gtgaaagtcg gcagcctgtc attttagtga caccctcggt
     8761 gctggcgtat ggcttggtca aattttggct gcacgattgt cattttcctg aatgaatttt
     8821 aaattacaaa tgccggcgct caaccaatct gaggaaaacg ttcgagtcct caaaggtctc
     8881 atcagccggt tcaatcagag tagtcttgag ttgatccggg agtatcggcg cctcgaagag
     8941 cgggcaggtt atctcaaagg acaacgtgag agcgtacgag agccgtgccg ccggggacag
     9001 aaaaatttcc acggccatta gaaacgtcaa gccaacggta agaggggaac agatctttcg
     9061 ttagtcccgg ttcgagatgt ctatggtcct gatggttgtc atagtggctc ctttgacttt
     9121 agccctcctg ctggaagcgc ggcatagccg cggcaactgc tgcgcatcgc atgctcaaga
     9181 atcgcaggcg ggcagggagg gctgaagtga gaggggtaat ggaattaatg aataacgagc
     9241 ttgccagagg agagcgcgaa acgtaattcg cccatccctg tggaatcttg ctgcgcaatc
     9301 ttatcatccg agtccacgat tgtctcagtt tggaaagcca tgaagttgct tcagttggac
     9361 gaggatcgaa tgctccgtgt ggagctctcg cttcaagaag tgttgctcag cgggcatttg
     9421 caaccgggag aataaaaacg aaaaagccgg ggttctgacc cccggcagga agtgctctgt
     9481 caggtctgaa agtaaagttt ccttgacgtt ttgttccatc aacgatcgtg ttcacctcca
     9541 tgcagaacct atttttgctt atggactctg ttccgaagtt gtcgtagtcc accgttagat
//
LOCUS       DASSWZ010000078         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_695413, whole genome shotgun sequence.
ACCESSION   DASSWZ010000078 DASSWZ010000000
VERSION     DASSWZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695413"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFN58_01975"
     CDS             complement(<1..203)
                     /locus_tag="VFN58_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916787.1"
                     /translation="MKITRVKSQIVKLPADEPLAGGPGFYRSFFEFVTLRMETDNGIE
                     GIGVTFFGWSLTPALKHAVDQLS"
     gene            complement(376..1110)
                     /gene="surE"
                     /locus_tag="VFN58_01980"
     CDS             complement(376..1110)
                     /gene="surE"
                     /locus_tag="VFN58_01980"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET8916788.1"
                     /translation="MRILIANDDGIYSPGIAVLAEVAAKFGDVRVVAPDVEQSSAGHS
                     ITAMRPLSYKRTPIGNFDAYRVNGTPADCVALGAYHWEKVDVVLSGINLGSNLGNAMW
                     HSGTLAAAKQAVLLGLRGIALSVAITRDEPNFDILKPSVEKVLGLLLEDRELTLVNVN
                     FPDVKPKGFRWTRQSVRHYDGKVVPGEDPLGRKLFWFTVVPIEGTEEDTDRWAVEHGY
                     VSMTPLRLDLTNEKELAGAQLRHPLD"
     gene            1192..2109
                     /locus_tag="VFN58_01985"
     CDS             1192..2109
                     /locus_tag="VFN58_01985"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET8916789.1"
                     /translation="MKVTLIHNPDAGDDRSPSGDELLDLIRRAGYSVGYQSSRDENWH
                     RVLEKPCDIVAVAGGDGMVGQVAKHLIDRHIPIAVIPMGTANNVAKTLGLTNKTITQV
                     IAGWKAGLLVNFDAGLASGPWDSKCFIEGLGMGLFTETMYRLDARDNAELAHLDDSEE
                     KVTSVLQILRERLRTFTPNELKIRLDGQDLSGQYILLEVMNISYVGPNLCLAPHAHPG
                     DGLLDIVFVSAGDQDKLGMCLSDCIDGKLARPALTVRKGQQLQVEWDGFTVHIDDEVW
                     PDKGSAFPLSPNFIDITVRRHALQFLVTP"
     gene            2326..>2817
                     /locus_tag="VFN58_01990"
     CDS             2326..>2817
                     /locus_tag="VFN58_01990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET8916790.1"
                     /translation="MFPYRDENETLRAAIVTGILIALNVLVWIVVQGAGSTVALARSV
                     CNLGLIPGELTSSLPVGTSFPIGDGLVCATDPGPQVSHLVTSMFLHGSWMHLLGNMWF
                     LWLFGNNVEDSMGRLRFLVFYLLCGLAAAMGQVVTNPSSAIPMVGASGAISGVMGAYL
                     VLYP"
BASE COUNT          632 a          738 c          761 g          686 t
ORIGIN      
        1 tcgctgagtt gatccactgc gtgcttgagc gccggcgtga gggaccaacc aaaaaaagtc
       61 acaccgatac cttcgatacc gttgtccgtt tccattcgta gggtcacgaa ctcgaaaaac
      121 gaacgataaa aacccggtcc gccggcaaga ggttcgtcgg ctggcaattt tacgatctgt
      181 gatttaacgc gtgtaatctt catgttaatc cttttcgatg ccgatcaaat ccctaaattt
      241 aagatcaaaa gtgatgcaaa tatccatcat ttgcgaaaaa cttgtcaagc ttctcgcgcg
      301 gagaaatgct gccatcttgc attgtcttcc gattggtcgt cagagcgatg aacgcactgt
      361 gtcgttgcgc gggcgctaat ccagggggtg ccttaactga gcgccggcca gttctttttc
      421 attcgtgaga tcgaggcgta gcggtgtcat tgacacgtaa ccatgctcga cagcccagcg
      481 gtccgtatct tcctccgttc cctcaatcgg aaccacggtg aaccagaaga gcttccgccc
      541 caacgggtct tcacccggca ccactttgcc gtcgtagtgc ctgaccgatt gccgggtcca
      601 gcggaaaccc tttggcttta catcgggaaa gtttacgttg accaaggtca actcgcggtc
      661 ttctaatagc aggccgagaa ctttttctac cgagggcttc agaatatcga agttcggctc
      721 gtcgcgtgta atggccacgc tgagagcgat gccacgcaag cccagcagga cggcctgctt
      781 ggcggcggcc aacgttcccg aatgccacat tgcgtttcct agattacttc ccagattgat
      841 tccggacagc actacgtcca ctttttccca gtggtaagcg ccaagcgcga cacagtccgc
      901 gggtgttcca ttgaccctgt aagcgtcaaa gttgccaatc ggtgtgcgct tgtacgaaag
      961 cgggcgcatt gcggtaatcg aatggccagc agaagactgc tcgacgtccg gagctacaac
     1021 ccgcacgtcg ccaaactttg ccgcgacctc ggcgaggact gcgattcccg ggctatagat
     1081 tccatcatca ttggcaatga gtatgcgcat tagtccaagc ctcctgtaag acttagcttt
     1141 atatttctac taacaatggt accatttatt ttcggcctgc atcatcaagt catgaaggtc
     1201 actttaatac acaatccgga cgccggtgat gatcgatcac cctccggcga cgagctttta
     1261 gacctcattc gccgcgcggg gtacagtgtt ggctaccagt cgtcgagaga tgaaaattgg
     1321 cacagggtcc tggaaaagcc ctgcgacatt gtcgcggtgg ctggtggcga cggcatggtc
     1381 ggtcaggtcg ccaagcattt aatcgacagg catatcccca tcgccgttat acccatggga
     1441 acggccaaca atgttgcaaa aaccctcgga cttacaaaca agacgatcac tcaagtcatc
     1501 gcaggttgga aagcggggct tcttgtaaac ttcgacgccg gcctggccag tggcccgtgg
     1561 gactctaaat gcttcatcga gggattgggc atgggactat tcactgagac tatgtaccgg
     1621 ctcgatgctc gagacaacgc agagcttgct catttagacg atagtgaaga aaaagttacg
     1681 tcggtcctgc aaatattaag agagcggctg cgaaccttta ccccaaatga actgaagatt
     1741 aggttagacg gacaggattt gtcaggtcaa tacattctgc tggaggtgat gaacatcagc
     1801 tatgtcgggc cgaatctctg tcttgccccc cacgcgcatc ccggtgatgg cctgctcgat
     1861 atcgtgtttg tctcggcagg cgatcaagat aagttgggta tgtgtctgtc tgactgtata
     1921 gatggcaagc tggcccgacc tgctctcact gtccgcaaag gtcagcaact gcaggtcgag
     1981 tgggatgggt ttaccgtgca cattgatgac gaggtctggc cggacaaggg ctcggctttt
     2041 cccctctcac caaactttat tgacatcacg gtgagacggc acgcacttca gtttcttgtg
     2101 acaccgtgag agccttgccg gacctccgag ataaaagctt gcgaatcatt ccagcagtga
     2161 cccacttgtg atagatgtca atctcatacc ggtgccttgg caagaaacat tcaagctgtc
     2221 aagtatgggt aagagcggcc ctaggttact gagtgcgagc gatgagttga cgctggcaag
     2281 cccgtttgct taggcaaatt attcggagct cgataagaag cacctatgtt tccatatcgt
     2341 gatgagaatg agacgcttcg cgcagccata gttacgggga tacttatcgc tctgaatgtg
     2401 ctagtctgga tagtggtaca gggggcaggc agcacagtgg cgctcgcccg atccgtctgc
     2461 aatcttgggc tcattcccgg agagttaacc agttcacttc cggtcgggac aagctttccg
     2521 attggcgatg ggttagtctg cgctaccgat cccggccctc aggtttcgca tttagtaact
     2581 tcgatgttcc tgcatggatc ctggatgcat ttgttgggga atatgtggtt cctgtggctc
     2641 ttcggcaaca atgtagagga ttccatggga cggctgcgct tcttggtctt ctacctcctg
     2701 tgtggactgg cggcggcgat gggacaggta gtgacgaatc ctagctcggc gattccgatg
     2761 gtcggcgcct cgggagccat cagtggggtc atgggcgctt acctggtact ttatccg
//
LOCUS       DASSWZ010000079        11010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_851188, whole genome shotgun sequence.
ACCESSION   DASSWZ010000079 DASSWZ010000000
VERSION     DASSWZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851188"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFN58_01995"
     CDS             complement(<1..367)
                     /locus_tag="VFN58_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HET8916791.1"
                     /translation="MSVKDVLDLVKKNKVEIVDFKFVDVPGLWQHFSIPAAELTADLF
                     EDGIGFDGSSIRGFQSINESDMLLIPDPDTAMMDPFTEHPTLSLICNVIDAVTRESYS
                     RDPRYVAQKAEKYLKSSGIA"
     gene            complement(456..1712)
                     /locus_tag="VFN58_02000"
                     /pseudo
     CDS             complement(456..1712)
                     /locus_tag="VFN58_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
     gene            1655..1801
                     /locus_tag="VFN58_02005"
     CDS             1655..1801
                     /locus_tag="VFN58_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916792.1"
                     /translation="MRCRLCRVSPVEQVPQRRGLKGELPKVFRLEPLVRFQAKSVDLL
                     LDQT"
     gene            complement(2136..3083)
                     /gene="ispH"
                     /locus_tag="VFN58_02010"
     CDS             complement(2136..3083)
                     /gene="ispH"
                     /locus_tag="VFN58_02010"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET8916793.1"
                     /translation="MAIRKVVIAQPRGYCAGVEMAVETVERALQRHGAPVYVFHEIVH
                     NQHVVQDFSRRGVRFVQSIDEVPRGARLIFSAHGVSPVVWQKAKEKQVEIVIDATCPL
                     VDKVHREVRKFAARGYWIILIGHEKHDEIVGTSGEAPERIRVVADVEDAKSIDIPEPE
                     KVVVLTQTTLSVDDTREVVDVLKARFPRMITPAKEDICYATQNRQDAIKQLAHEVDLV
                     LVFGSRNSENSNQLCKVARACGTTAYLIEDRHAIDPTWLVGVERIGITSGASAPERLV
                     QETADFFKHQGAEITYLGFAEENIHFALPPEVSSENLPH"
     gene            complement(3145..4215)
                     /locus_tag="VFN58_02015"
     CDS             complement(3145..4215)
                     /locus_tag="VFN58_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HET8916794.1"
                     /translation="MSSILYYITGHGYGHAVRSSQVIGSLKKTSPDLRIYVRTTAPEW
                     LFLNRALLSRQAIDVGIVQKDSLEMDLDGTLRACQSLHKSVPRLIEQETNFVREHQIR
                     LILGDIPPLCFEIAMRASVDSVAIANFTWSEIYGAYIKNCPGFKPLIEEMEGFYRKAT
                     LALTLPYPCGMDVFPRKEPIPWVARRSALTKEEARKAFRLPQSATVVLLSFGGLGLER
                     FPWGKLKAMSELFFVTTGENKQETNNLRVLPEAQRNYADLVRAVDVIVTKPGYGIVAD
                     IISHQVPTLYTDRGDFAEYRYLVQALEDCATAEFIPQDQLLSGNLVPYLTRLLDKKEN
                     WLTVPLDGAEVATAKILRLIGP"
     gene            4335..4538
                     /locus_tag="VFN58_02020"
     CDS             4335..4538
                     /locus_tag="VFN58_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET8916795.1"
                     /translation="MTGTIKKIIRQKGFGFITPDDGSEDVFFHRGSLAPRTQIEDFNE
                     GDTVQFQTRKGDKGPVALDLKIR"
     gene            4569..4865
                     /locus_tag="VFN58_02025"
     CDS             4569..4865
                     /locus_tag="VFN58_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916796.1"
                     /translation="MDEFQSLSANRLLFDLASRLGSLEGYLYAEDKVDKSYLPNWIDN
                     IDHEFRNLSAEVRNEIQADYLALLKKVRDLLHKAYGDQDPNTAKIDAAISRIAQ"
     gene            4879..5847
                     /locus_tag="VFN58_02030"
     CDS             4879..5847
                     /locus_tag="VFN58_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916797.1"
                     /translation="MRRHDFAGFARIAAGLCLLLLSPVVATNGAAAEKFFISIPGPTL
                     SYVPLYYAQEKGFFLQEGLDLHVLVVRGIIGVSSLMSGEIDVTCHAGSGFSAALRGIP
                     IKIISVTRDRPIHELIVGPSISSSTDLKGKAIAVGSLEGTAAVMTRRILQAKGLDAQK
                     DVTLLSMDTPARLQSLMTGKVSGGMMTPPSTYLAMDQGYKVFGRGRDYMRFLQTGVVA
                     TDTYIKHKREPLVRFLRAWNRALKFYQDNPEVMLPYIQRKLGVKDAQLARRMYDDDAP
                     YILPGGRLSPDAMKEIVEIGREALKIKEPISTDRVFDFSLASEALK"
     gene            complement(5867..8533)
                     /gene="polA"
                     /locus_tag="VFN58_02035"
     CDS             complement(5867..8533)
                     /gene="polA"
                     /locus_tag="VFN58_02035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET8916798.1"
                     /translation="MSAKKPITKSNPNASVGATSEGRKAILLDGHSLAFRAFFALPDT
                     LVTSSGQITNAVYGFTAMLIKLLADERPDAVVVCFDRGAPQFRLDRYAEYKAGRAETP
                     DTFRQQLPLIREVLASLRIPMVELEGYEADDLLATLTKYFKQERNEVVIVTGDRDILQ
                     LVGEGVSVIMTRRGISDVIRYDNATVLERYGVTPEQWTDFVALKGESSDNLPGVPGVG
                     DKTAAQLVNKYGDIEHVIAHADELTPKLRDAIKERAHQVRINKELGRLLDDVPLAIDA
                     ADLRLEPWDEEEVRKLFTSLEFRTLHERLKELKLHASAAPPALELKTISELRSETDLR
                     PGEAVALAWSAEWLALCDEPGEARILPFSSGMQRLEGFLNDPNRQKIAHDAKPLYRSA
                     LAAGTEIQGLYCDTKIAAYLLEPGSAPGYTVRDTVSRYLKISIDEAPPELQAGEQACL
                     TLGEDDKRRVSREAVAVRAVAPALEEQLSNQGIWELATTLEFPLVKVLARMEHNGILV
                     DRGYLEKLNSEFGRKMATLEDTIQRLADENFKVNSQPQLQRILFEKLGLPKTRKIKTG
                     YSTDAAELEKITGAHPIIASLLEYREVSKLKTGFTEMLLELIDPKTNRIHTTYEQTTA
                     ATGRLSSTAPNLQNIPIRGEMGRQIRRAFTAPPDHVLLSADYSQIELRVLAHLSQDVA
                     FLQAFAQGHDFHATIAGKVYGVAVNAVTPEMRNHVKQFSYGIAYGMSAFGVSQRLGVE
                     MDEAGNFIEAYYAQFPKVREFLTAQVEKAKIDGFTTTMFGRRRYLPELGSSNYRLRAL
                     GERMALNAPIQGSAADIIKQAMISVDDALQREPVAKMLLTVHDELVFEVPASKLAQAQ
                     RLIEEKMTQAVKLQCGLAVEVHTGTNWAEAHA"
     gene            complement(8689..9171)
                     /locus_tag="VFN58_02040"
     CDS             complement(8689..9171)
                     /locus_tag="VFN58_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916799.1"
                     /translation="MTPKKIDCSYLDVVQVLFPLLCGRVFHVTTEKKFTDICRSGWIY
                     SKEQTQFVLTPGQSETTYGRKRGWVSLYDLSYTEHTDIKEALIRYWFFRTLRAGRTDV
                     YLMIAKNAWSSLVSWKCASRDVGGQEFFVPFVEAWYPGDIPLQLVNESLMVSVHPPSR
                     "
     gene            complement(9687..10670)
                     /locus_tag="VFN58_02045"
     CDS             complement(9687..10670)
                     /locus_tag="VFN58_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET8916800.1"
                     /translation="MKSFENIDAKSVNQAVALLQKFNQEKKNAIVVGGGSEILQLMKD
                     QVVTPDYVVNLKTIPGLNTIKEERSGFRIGGLTTLADIEEHSGIREKLLILSDAAGEA
                     ASPQIRNAGTIAGNICQRPFCWYFRSANFNCLRKSGQVCYTVTGDGRFHAILGAGPSY
                     IVHPSDTAPALVALDAQIKIAGPSGERVMPLEKFFVLPSVDFKRENILKPSEIVTEIF
                     VPYPKSGSKGYYHKVRERLAWDHAIVSVATIVQSNGGVARDARVVLGGVAPIPWRAPK
                     AEEFLRGKKIDEETAQKAGEIALEGARPLKDNVYKVEMAKSLIHRALLASV"
     gene            complement(10686..>11010)
                     /locus_tag="VFN58_02050"
     CDS             complement(10686..>11010)
                     /locus_tag="VFN58_02050"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8916801.1"
                     /translation="ESQIKGGVVQGIGMAFTEELITDRVTAIPINPNYRDAKVPTHLE
                     TPEVEVIFIEHPDPFGPFGGKVVGEPPITAAVATIANAIFNATGKRIKELPITPDKIL
                     SARQV"
BASE COUNT         2580 a         3000 c         2843 g         2587 t
ORIGIN      
        1 cggctatgcc gctcgacttt aagtacttct cggccttctg ggctacgtaa cgcggatctc
       61 tggagtagga ctcccgagtc acggcgtcga taacgttaca gattaagctc aacgtcgggt
      121 gttcggtaaa cgggtccatc atggcagtgt cgggatcggg aattaacagc atgtcggatt
      181 cgttgatcga ctgaaatccc cgtatgctcg agccgtcaaa gccaatcccg tcttcaaata
      241 gatcggccgt cagttctgcc gctggtatcg aaaaatgctg ccataacccc ggcacgtcga
      301 caaatttaaa atccactatc tccactttgt tcttcttgac taagtccaaa acgtccttga
      361 cactcatcta actactcctc tcttcaacaa taacttttcg accgcaaatt ttgttatcac
      421 attctttcat caacagctag cccgcggcgt gatgattact catacgcgcg ctcgttgtgt
      481 tggctcaagt ccaagcccat gcgttcgtca tcatctgcta ccctgagact catgatcgcc
      541 ttaacgatgg acagaagaat gtaagttcca acgatagcga gaatccaagt ggctaccaca
      601 gcaacgaatt gaacccaaag ctgccccgga ttgccgaaga agagaccgtt atttccggcc
      661 gcgtttatgg ctttggaagc gaaaagacct gtcgccagcg ctccccaggt tccgccgacg
      721 ccgtgtactc cgaccacatc tagcgagtca tcgtaaccga gttttggctt tagactacag
      781 gccatggagc aaagtactcc gccacctatc ccgatccaga ttgctgacat gggaccgaca
      841 aatcctgatg ccggtgtaat ggcaaccaac cccgcaaccg cgccgctagc ggctcccaag
      901 catgtcggtt tccctctgaa aatccagtcc atcgccatcc aggaaagggc tgctgatgct
      961 gttgcgatgt gggtagctac gaatgcagac gtagctaaac catcagccgc tagcgcgctg
     1021 ccggcgttga aaccaaacca acccacccac aaaagcgagg cgcctatgag actgaacgga
     1081 aggttgtgcg gcggcatcgc ctcctggccg taaccgattc gccgcccaaa catgagagcc
     1141 gccacaagag ccgagacccc tgagctaatg tgaactacag tgccaccggc aaagtccagt
     1201 gcccccatct cacgcatcca tccctttaca ccccataccc aatgagcgag tggatcatag
     1261 atgaacgttg cccacagcag aatgaaaacc aggaaggcac caaatttcac gcgttccgca
     1321 aaagccccgg tgataagcgc gggagtaatg atcgcgaaca tcatctgata gaccatgaaa
     1381 gtctgatggg gaatcgtagc agcgtagtcg gcgttcggtt ccgtaccgac tccgttcagg
     1441 ccaaaccagt cgagatttcc gacaattcca cctacgtccg gtccaaacgc cagactgtag
     1501 ccccagagga cccattggat actgattacg gcgacaatga taaagctatg catgatagtg
     1561 ccgaggacat tttttcctct aaccaggcca ccgtagaaga gaaagaggcc tgggatcgtc
     1621 atcatcaaca ccaaagcaga ggaagttaac atccatgcgt tgtcgccttt gtcgagtttc
     1681 gcctgtggag caggtgccgc agcgccgggg gctgaagggg gagctaccga aggtttttcg
     1741 gttggagccg ctggtgcgct tccaggcgaa gtcggttgat ttgcttcttg accaaacgta
     1801 atagccggaa ccgttagcgc taatacccat aggaggctaa agacaatccc ccagtttcta
     1861 ttctgttgat tcacgaccag tcctcctttt tttgcacaaa aagtaagcta acgagcgaat
     1921 aaaaaggtaa ttttaagcat gcgcggcaac atatgtgcca tcgacaggaa ttgaatttat
     1981 ccctttttat tggctacatg tggagtggat gcgaaagcca tgaagaacgc ttttgcccaa
     2041 ttagcaggca aatgcacaag aaatagtcat cagtgccgcc agcgtcgaac taacgaagag
     2101 cagcacaccc tgaatgttgt aaatggcaag ccggatcagt gtggcaggtt ttctgaactg
     2161 acctcgggag gtagagcgaa atgaatgttc tcttctgcga aaccaaggta ggtaatttca
     2221 gcaccctggt gtttgaaaaa atcggcggtc tcctggacca acctttcagg agctgaagca
     2281 ccggatgtta tgccaatccg ttccactccc actagccagg tcggatcaat ggcgtgacga
     2341 tcctcgataa gatacgcggt cgtcccacaa gcgcgcgcca ctttgcaaag ttggtttgag
     2401 ttttcgctgt tacgcgatcc gaacaccaga accaagtcta cctcgtgagc cagttgcttt
     2461 atggcgtcct gtcgattttg agttgcataa cagatgtcct ctttggccgg cgtaatcatc
     2521 cgtgggaacc ttgccttcaa gacgtcaacc acttcgcggg tatcatctac gctcagtgta
     2581 gtttgagtga gaacaacgac cttctcaggt tccggaatat cgatagactt cgcgtcttca
     2641 acgtcggcta cgacgcgaat tcgctctggt gcttcgccac ttgttccgac gatctcatcg
     2701 tgcttttcgt gaccgatcaa tatgatccag tatcctcgtg cggcaaattt tcgtacctca
     2761 cggtggacct tgtcgaccag agggcaggta gcatcgatga ctatttccac ttgtttctct
     2821 ttggctttct gccacaccac tggagagacg ccatgagcgc tgaaaatcaa tctggcgcct
     2881 ctaggcacct catcgatgga ctgcacaaaa cgcaccccgc gccggctaaa atcttggact
     2941 acatgctggt tgtgaacgat ttcatgaaac acgtaaaccg gtgcgccgtg tcgctgtagt
     3001 gctcgttcca cagtctcgac ggccatctcc acgcctgcac agtaaccgcg cggctgtgct
     3061 atgacgactt tgcgtattgc cataagtatg cttcaagtct ggtagattga taaggctctg
     3121 tcaatactgg atccctgcat ggattcaagg accgatcaag cgcaaaatct tcgctgttgc
     3181 cacttctgca ccgtccagag gtaccgtcag ccagttttcc ttcttgtcca acaggcgggt
     3241 caagtacggg acgagattcc cggataacag ctgatcctgg ggtatgaatt ccgctgtcgc
     3301 gcaatcctcc agagcctgca ccaaataccg atattcagca aaatcacccc ggtcggtata
     3361 cagtgtcggc acctggtgag aaatgatgtc cgcaacgatg ccatagccag gtttggtaac
     3421 tataacatct acggcgcgaa ccagatccgc gtagtttcgc tgggcttccg gcaagacgcg
     3481 cagattgttt gtttcttgtt tgttttcccc agtcgtcaca aagaacaact cactcatcgc
     3541 cttcaatttg ccccagggaa accgctccaa ccccaaaccg ccaaagctca agagcacaac
     3601 ggtagcagat tggggtaagc gaaatgcttt cctcgcctct tcctttgtca gggcagacct
     3661 acgggcaacc catgggatgg gttcttttcg cggaaagaca tccatgccgc acggataagg
     3721 aagggtcaaa gccaaggttg ctttgcgata aaacccttcc atctcctcaa tgagcggttt
     3781 gaagccggga cagttcttga tataagcgcc atagatctca ctccatgtga aatttgcgat
     3841 cgccacagaa tcgaccgacg cccgcattgc gatctcgaaa cagagcggtg ggatatcacc
     3901 cagaatcagg cgaatttgat gttctctgac aaaattggtt tcttgctcaa tgaggcgcgg
     3961 tacgctcttg tgcaatgact ggcaggctcg caacgttcca tcaagatcca tctcgagact
     4021 gtctttttgt actattccaa cgtcgatcgc ctgacgagat agaagggccc gatttaggaa
     4081 gagccattca ggtgccgtgg tacgaacgta aatcctcaga tctggagagg tctttttcag
     4141 actgccgatg acttggctgg aacgaactgc atggccatac ccgtgcccgg taatataata
     4201 aagaattgaa ctcaagttgg cttttggatg tatgtactcg gaaacattaa cctagggagc
     4261 gagccaagaa ttgtcaagga cctttccgct tgaagagtct cacgcaactg acataggaag
     4321 aggagaagtt agccatgacc ggaaccatta agaagatcat tagacagaag gggtttggat
     4381 tcatcactcc cgacgacggc agtgaagatg tattttttca tcgcggaagt ctcgcgcccc
     4441 gaactcagat tgaagacttt aacgagggcg acaccgtaca gtttcagaca cgaaagggtg
     4501 ataagggtcc tgtcgccttg gatctgaaga ttagatgaac cggatcccag gcacaaagga
     4561 gagtactgat ggatgagttt caatccctat cggccaaccg tctacttttt gatctggcgt
     4621 cgcgattagg ttctttagag ggctatttgt atgccgaaga taaagtcgac aagagttacc
     4681 tgcccaattg gattgacaac atcgaccatg aatttagaaa tctctccgcc gaagtgagaa
     4741 acgaaattca ggcggattat ttggcgctgc taaagaaagt tcgagatctg ctccacaagg
     4801 cttacggcga ccaggatcca aacaccgcaa aaatcgacgc agcgatttct aggatagccc
     4861 aataaaagga gcctaaaaat gaggcggcat gatttcgcag gcttcgcccg gatcgcagcg
     4921 gggctttgtc ttcttttgct ctcgcctgtc gtcgcaacaa atggagcggc ggcagagaag
     4981 ttttttatta gtattccggg gccgactcta agctacgttc ctctttatta cgctcaggaa
     5041 aaaggcttct ttttacagga aggtctggac ctgcatgtat tagtggttcg gggcataatc
     5101 ggtgtcagca gccttatgtc cggtgagatc gacgttacct gccatgccgg aagcggtttc
     5161 tccgcagctc ttcgagggat tccgatcaag atcatctcgg ttacccgcga tcgtcctatc
     5221 cacgaactta tcgtcgggcc atcaatcagt tcgtccaccg acctaaaagg aaaagccatc
     5281 gctgtaggtt ctttggaggg aaccgccgcg gtcatgaccc gccgtattct tcaggctaag
     5341 gggctcgatg cgcagaaaga tgttactttg ttgagcatgg atacccctgc gagactgcaa
     5401 tccctcatga ccggcaaagt ctcaggtgga atgatgactc caccttccac ttacctggcg
     5461 atggatcaag gttacaaagt ttttggccgc ggccgcgact acatgcgctt cctgcaaacc
     5521 ggcgttgttg ccaccgatac ctacatcaaa cacaaacgcg aaccgctggt gcgttttctg
     5581 cgcgcctgga accgggcact aaaattctac caggacaacc cggaagtcat gcttccttac
     5641 attcaaagga aactcggcgt gaaagatgcg caattggctc gccgaatgta tgatgatgat
     5701 gcgccttaca ttctacccgg cggccgcttg agtccggacg caatgaaaga gatcgtggag
     5761 atcggtagag aagctttgaa aattaaggag cccatatcga cggatcgagt tttcgacttt
     5821 tcattggcca gtgaagccct taagtgattg ccgctcgcga agcaactcaa gcatgcgctt
     5881 ccgcccaatt cgttccggta tgcacctcaa cagcgaggcc gcactgtagc tttacagcct
     5941 gagtcatctt ctcttcgatc agccgctgcg cttgggctag cttgctggcc ggcacttcga
     6001 acaccagttc gtcatgtaca gttagcagca tctttgccac cggctccctc tgcagggcgt
     6061 catcgacgct aatcatcgcc tgcttgatga tgtcggcggc gcttccttga atcggcgcat
     6121 tgagcgccat gcgctcgccc agcgcccgca accgatagtt tgacgaaccg agctctggta
     6181 agtagcggcg tcgaccaaac atcgttgtcg tgaaaccgtc gatcttggct ttttccacct
     6241 gagccgtgag aaactccctc actttcggaa actgagcgta gtatgcttcg atgaagtttc
     6301 cagcctcatc catctcgacg ccgagtcgct gtgaaactcc aaaagcggac atcccatagg
     6361 cgatcccata ggagaactgt ttgacatggt tccgcatctc aggcgtgacc gcgttcaccg
     6421 caacgccata aaccttgccg gcgatggtcg catggaagtc gtgtccctgc gcgaatgcct
     6481 ggagaaaagc cacatcctgg gaaagatgcg ctagcactcg gagctcgatc tgcgagtaat
     6541 ccgccgagag cagcacgtga tcgggaggtg cggtaaaagc gcgccggatc tggcggccca
     6601 tctcgccgcg aatcggaatg ttttgcaggt taggcgccgt tgacgacaag cgtccggtgg
     6661 cggcggttgt ctgctcatag gtcgtatgga ttcgattcgt tttgggatcg atgagctcca
     6721 gcagcatctc ggtaaaaccc gttttgagct ttgaaacctc ccggtattcg agcagtgaag
     6781 cgatgattgg atgagcacct gtaatcttct ccaactccgc agcgtcggtg gaatagccgg
     6841 tcttgatttt cctggttttt ggcagtccca gtttttcaaa gagaatccgc tggagctgcg
     6901 gctgggagtt taccttaaag ttctcgtccg cgagccgctg aatcgtgtcc tcgagcgttg
     6961 ccattttccg accaaactcg ctgttcaact tctcaagata cccgcggtcg acgagaattc
     7021 cgttatgctc catgcgcgcg agcaccttga ctaagggaaa ctccaaggtg gtcgcaagct
     7081 cccagatccc ttgattgctg agctgctcct cgagagcggg ggcgacggcc cggaccgcca
     7141 ctgcttcccg ggaaacgcga cgtttatcat cctcgcctaa agtaaggcag gcctgttctc
     7201 ccgcttgtaa ttccggcggg gcttcatcga tggagatttt gaggtaacgg ctcaccgtgt
     7261 ctcttaccgt gtaccccggc gcagagcctg gctcgaggag ataggcagcg atcttggtat
     7321 cacaatagag cccctgaatt tccgtccccg cagcgagggc cgagcggtat agcggcttgg
     7381 catcatgagc gatcttttgt cggttcgggt cgttaagaaa accctcgagc cgctgcatcc
     7441 ccgacgagaa aggtaaaatg cgcgcttccc ccggctcgtc acacagagcc agccactccg
     7501 cactccaagc gagcgcaacc gcctcacctg ggcgaagatc tgtctctgag cgaagttctg
     7561 agattgtttt gagctcgaga gcgggcggcg cggcagaggc gtggagcttg agctctttca
     7621 accgctcgtg cagagtgcgg aactcaagag aggtgaagag cttgcgcact tcttcctcat
     7681 cccaaggttc gaggcgaaga tccgcggcat cgatagccag cggcacatca tcgagcagac
     7741 gcccaagctc tttattgatc cgcacctggt gcgcgcgctc tttgatggcg tcgcgcagtt
     7801 ttggcgtgag ctcgtcggcg tgggcgatga catgctcaat gtcgccgtat ttattgacca
     7861 gctgggcggc ggtcttgtcg cccacacccg gaacgccggg caaattgtct gagctctcgc
     7921 ctttgagcgc cacgaagtcc gtccattgct ccggcgtgac cccgtagcgc tctagcaccg
     7981 tcgcattgtc gtaacgtata acatcggaga ttccccggcg cgtcatgatc accgaaaccc
     8041 cctcgccgac caactggagg atatcccggt cgccggtgac gatgacgacc tcattccttt
     8101 cctgcttaaa gtacttcgtc agagtcgcca gcaaatcgtc ggcttcgtaa ccttcgagtt
     8161 cgaccatcgg gatgcgcaag cttgcgagca cctcgcggat caaaggaagc tgttgccgaa
     8221 aagtgtcggg tgtttcagct cggccagcct tgtattccgc gtagcgatcg agccggaact
     8281 gtggtgctcc tctatcaaag cagacaacaa ccgcatccgg acgctcgtct gccaaaagtt
     8341 tgatgagcat cgcggtaaaa ccatagaccg cattggtgat ctgccctgat gaagtgacaa
     8401 gtgtgtccgg taaggcgaaa aaggcgcgga acgctagcga atggccgtca agcaatatcg
     8461 ctttgcggcc ttcgcttgtg gcaccgacgg atgcattggg attggatttt gtgattggct
     8521 ttttcgcgct catgttttgc agtctacccg ttgctcacag cggcagcaat acgcgacaaa
     8581 gcaatacgac gtcgacattg tcgtcaaagg acaaatcccg aaaggaacgg cctgagtcgt
     8641 ggtaattccc gcagtgggcc ggattgaatt tttatcgttt taggaagctt agcgggaggg
     8701 cggatgaaca ctaaccataa ggctttcgtt aacgagctga agcggaatgt cgccgggata
     8761 ccaagcttcc acaaaaggaa caaaaaattc ctgtcccccc acgtctcgac tggcgcattt
     8821 ccaggaaacc aaggacgacc atgcgttttt ggcgatcatt agataaacat ctgttctccc
     8881 cgcgcgcagt gtcctgaaga accaatagcg tattagtgcc tccttgatat ccgtatgttc
     8941 tgtatagctt aagtcgtata ggctgaccca accccgcttt cgaccgtagg tagtctcgga
     9001 ttgacctgga gtgaggacaa attgggtttg ctccttgcta tatatccatc cagatcgaca
     9061 aatatcggtg aattttttct cagtcgtcac gtggaacacc cgcccacaga ggagaggaaa
     9121 cagaacctgt accacgtcca agtaactgca atcgattttt tttggtgtca attccaaatt
     9181 tacacgcaac cggttttcga gatgacgacg gttgacgcgc aaaccgcatg ccattgccaa
     9241 gatggctgaa ttttctaagt ttccaatccg gctggatgaa aaaaaatttc cctgaccacg
     9301 taaaaaatac ctacggtcgc aggataagcc ctccactact gcattattag taatgtcttc
     9361 gtctggacgc atgctcgaga tctacgagac agtttcgttc tgcaactaag agattacctg
     9421 aggtgctttc aggagttcgc gcgtgtccat gtaagggaac gaaaagcttt gcatcagcgg
     9481 tccgaactga tgcagcctac aagcaacccg acatgggatt gccgagcttg catcccacgt
     9541 aaaaggtttt ttattgaaaa gtgtggtcga tagttgaagc gtttgtccat ttcctccgga
     9601 gcgtcatggc aaagcaagaa gatgaaggta tgtttcccct cacgcatgcc gctcctggct
     9661 acgcttttgg ccaagagcgg gtcaacttac acagatgcga gcagcgcgcg gtggatcaac
     9721 gacttcgcca tctccacttt gtagacgttg tcttttagcg gcctggcgcc ttcgagggca
     9781 atctcgcccg ccttctgagc agtttcctca tcgattttct tgccccggag aaactcctca
     9841 gctttcggcg cgcgccacgg aatgggtgcc acgccaccga gaaccacacg tgcgtccctc
     9901 gcgactccac cattgctctg cacaatagtc gcgacgctca cgatggcatg atcccaggcc
     9961 aaccgctcac gcactttgtg gtaatagccc ttgctcccgg acttgggata aggaacaaag
    10021 atttcggtga cgatctcgct gggcttgagt atattttcgc gtttgaagtc cactgaaggc
    10081 aggacgaaga acttctccaa cggcatgact cgttcacccg aaggtccggc gatcttaatc
    10141 tgcgcgtcga gtgcgacgag cgccggcgcc gtgtccgacg gatgaacgat gtagcttggt
    10201 ccggcgccca agatggcgtg aaagcggcca tcaccggtca cggtgtagca aacctgcccg
    10261 ctcttgcgta ggcaattgaa gttagcagaa cggaaatacc agcagaaagg ccgctggcaa
    10321 atgttgccgg cgatggtgcc ggcattgcgg atttgcggcg aagccgcttc cccggcggca
    10381 tcggacagga tcagtagttt ttcacgaatt ccagagtgct cttcgatgtc cgccaacgtg
    10441 gtcaacccgc cgatacgaaa tccgctgcgc tcttccttaa tcgtgtttaa acctgggatg
    10501 gtcttcagat tgacgacata gtccggcgtg accacctggt ccttcatcaa ctgcaaaatc
    10561 tcgctgccgc cgccgaccac gatggcgttt ttcttttcct ggttgaattt ctgcaacagg
    10621 gcaacggcct gatttacact cttggcatcg atgttttcaa aacttttcat ggccttcctc
    10681 ctccatcaga cctggcgagc cgacaagatt ttgtccggcg taatgggcaa ctctttgatt
    10741 cgttttcccg tggcgttgaa gattgcattg gcgatggtcg ccacggctgc ggtgatgggt
    10801 ggctcgccga caactttgcc gccaaaggga ccaaaggggt cagggtgctc gataaagatc
    10861 acttcgactt ctggcgtctc tagatgggtc ggcactttgg cgtcgcgata gttggggttg
    10921 ataggaatcg cggttacgcg atcagtgatg agttcctcgg tgaacgccat gccgataccc
    10981 tgcacgacgc cgcctttgat ctggctttcg
//
LOCUS       DASSWZ010000080         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_766293, whole genome shotgun sequence.
ACCESSION   DASSWZ010000080 DASSWZ010000000
VERSION     DASSWZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766293"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFN58_02055"
     CDS             <1..219
                     /locus_tag="VFN58_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017528080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET8916802.1"
                     /translation="RAERTMMKIVVDHTTDKVLGCHMVGADAGEIIQGMAVAMNCGAT
                     KAQLDATIGIHPTAAEEFVTMRTAVAHT"
     gene            536..1285
                     /locus_tag="VFN58_02060"
     CDS             536..1285
                     /locus_tag="VFN58_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET8916803.1"
                     /translation="MDRETFREQLLAVMERKDHWAWPAFTSGRVPKALLHFHFEQEYE
                     TYVRDFPVMVGWAYVRCPVPEVRRSLAENLYEEETGGLVAGSPHPELFLEYPRGLGMD
                     LSRFQHVELLPAANEYRHFLDEAIRHYGWEIAAAVVTIFIEGTKDERSVIDPSGPKVP
                     PPPFEEHPLVKHYGMALEHLALTKAHRRVEGNHRAAAWHAILTHISPTRRGPVVRAMN
                     EALDRWLSYRDEVAVVCGIAPNGDNSAASAA"
     gene            1419..1892
                     /locus_tag="VFN58_02065"
     CDS             1419..1892
                     /locus_tag="VFN58_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916804.1"
                     /translation="MNKKSIIIGVGAGMISILALANQASADTRRFDYPRYNRPAHQEI
                     RGDRKELFKDRVELRRDLRELHKDKAELRRDIRRGASPEEIARGRAEVRQGVREVWQD
                     RREIRQDHGELRRDLDKYGWNRYPNYNGRYGYAGNRYSGWDRNRWGWDRNRWDRD"
     gene            2093..3271
                     /locus_tag="VFN58_02070"
     CDS             2093..3271
                     /locus_tag="VFN58_02070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916805.1"
                     /translation="MIIDCDTHLMPREAFDGVDGEFGRRKPTLKFNTDGLYVDVEFPG
                     YPPEVSGTSPLLAPGSGAMFKSLWDVESRMTDYDKKLGIEQHVVLPQFSGWWSYLIEA
                     ELAARMARSHNEALLRVMREYPGRILGVALVALQDVAGSIRETEWARENGFCAVVLDK
                     VFPVKDHPYGEPLGSHRELWPFFKRAEELSMPIYLHNVQHGHRISNFSLFQRDGLYVF
                     APEEGQMSLVSLITSGLLDEYPRLQFIYTEAGTSFIKPLVVRLDKLLEAPPIDYADQE
                     EPLGNRPLTKVGEKLRRARALNPAHIFLEKNKKPASHYFKNNFYFTIETEEPELPEAI
                     EFLGAGRFLFATDYPHDDPGGRMKFQDVELLRNNPKISESDKELIRWRNAERLFHLAL
                     "
     gene            3295..3783
                     /locus_tag="VFN58_02075"
     CDS             3295..3783
                     /locus_tag="VFN58_02075"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET8916806.1"
                     /translation="MAIPERYNTGYNIGVGGAVVRGGQLLLVRRASRHGHGNWQLPGG
                     FIEPDETIEQAVVREVQEEAGVKGEVEAVLGLRSRYDPDSGNGIYIVLLLRPVSGEPT
                     ADGHEVDHAAYFTLEQIRDLNPLPPVNWEIAQRVLDPTRRLLLPKPLTNLNGAKFTLF
                     VG"
     gene            complement(3870..>4759)
                     /locus_tag="VFN58_02080"
     CDS             complement(3870..>4759)
                     /locus_tag="VFN58_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916807.1"
                     /translation="IYQKEPLQRESIVGIQPESSFREKNETDRLAPSVAPAPTSKTKG
                     VAEETKPRLQEFKDSIQVKDLQSRPKPEEQDNAPETARPQDQVRSPATFSPVPLQEKS
                     SAASEAAFPRREQSSSSREAQSKEALTPTPPPVSAAKSSFSPEARERSAASSLDTLRS
                     GTVVGVAVPADHELAIRLKEPVRDDKAMGDRLASGGAQAVRRSLTSQEEVKNLDQARQ
                     RAIQTGQSQTAWVTIARNQYELFKKERADLGNIEVESSTPDRNNDAIAKSSDQLRIKV
                     TILPPLPSGNPVPSQPSSR"
BASE COUNT         1057 a         1210 c         1367 g         1125 t
ORIGIN      
        1 cgggctgaaa ggactatgat gaaaatcgtc gtagatcaca caacggataa agttttgggc
       61 tgccacatgg tcggcgcgga tgccggggaa atcatccagg ggatggccgt ggcaatgaat
      121 tgcggcgcca ccaaggccca actcgatgcg accatcggta ttcaccccac ggcagcggaa
      181 gagttcgtga ccatgcgcac ggcggtggca catacgtaga ccggtctatt caccgaaagc
      241 ccgcgccttc gaagacagcc tacttgcgtc tgtttgcttg cacccttcgg ggggaacccc
      301 tcggtctact ccctgtcgta atctttcttc ggactatgac cgggcagtgc gttgtctgaa
      361 atcggttgag atcgctgcct acgtacgccc gccaagttgt cttgttttct gacaagtgtc
      421 cgctttgccc gatcgcgctc gtcggcaaaa tcctgaaaag tcagcccctt tgatagtggc
      481 ataatggttg ctcgtcgccc ttattagaag aggccggtaa ccaaaagagg tggctatgga
      541 tcgggaaacc tttcgggagc aattgttggc cgtaatggag cggaaagacc actgggcctg
      601 gccagcgttt acgtcgggga gggtacctaa ggcgctgcta cattttcatt tcgagcaaga
      661 atacgaaacc tacgtgcggg attttccagt catggtgggc tgggcttatg tgcgctgtcc
      721 ggttcctgaa gttcggcgaa gcctggctga aaacctttat gaggaagaga ccggcggtct
      781 cgttgccggc agcccgcatc ctgagctttt tctcgaatat cctcgcggac tggggatgga
      841 cctaagtcga tttcagcacg tcgagttact gccggcggcg aatgagtatc gccacttcct
      901 cgatgaggcg atccgacact atggctggga gattgccgca gcagtcgtta ccatttttat
      961 cgaaggcacg aaggacgaac gctccgtgat cgatccatca ggaccaaaag taccaccgcc
     1021 gccctttgag gagcatcctc tggtcaaaca ttacggtatg gctctggagc acctcgccct
     1081 caccaaagct catcgtcggg ttgagggtaa ccatcgtgcg gcggcatggc acgcaatcct
     1141 gacccatatc tcaccaacca gacgcggtcc agtcgtacgg gcgatgaatg aggccctaga
     1201 tcgttggctc tcgtaccgtg acgaggtcgc ggttgtctgt ggtatcgcgc caaatggcga
     1261 taattccgcc gctagcgccg cctaaggctt tttagaacct tccgtcacaa tccctcaatt
     1321 ttgccaaatt cttaatgcgg ctgacggcga tgatcgccgt tactaccgtc actaaacatt
     1381 tccgacacac gcacgtctaa ttggccaagg aggtgcctat gaacaagaaa agcataatca
     1441 ttggtgtagg cgctggaatg atttctatct tggcgttggc gaatcaagcg tctgcggata
     1501 cgagacgttt cgactatcca aggtacaatc gaccggcgca tcaggagatt cgcggcgacc
     1561 ggaaggaact gtttaaggat cgagtggaac tgcgcagaga tttgagagag ctccacaagg
     1621 acaaggcaga gctccgtaga gatatacgcc gcggcgcgag tccggaggag attgctcgag
     1681 gcagagccga agtcagacag ggcgttcgcg aggtttggca ggaccgtaga gagattcgcc
     1741 aagatcatgg ggaactgcga cgcgatttag ataagtatgg ctggaataga tatcccaact
     1801 acaacggaag gtacggctat gcgggaaatc gttacagcgg atgggatcgc aaccgttggg
     1861 gttgggatcg taaccggtgg gatcgcgact gaaaaaggaa gcagtaacct agttgacaat
     1921 tagaccaacc ttaaccttcc aacaactgcg atctcgcctc tccggaggcg ggcaaccagc
     1981 ccgcctccgt tcctttcaga tcgactctgt aaaaccggac cttcttgaca tcgaaaatca
     2041 tggagtccta taaggttaaa aggacaattt tttctacaag ggacggacaa tcatgatcat
     2101 cgattgcgac actcacctga tgccgagaga ggcctttgac ggagtagacg gagaattcgg
     2161 ccggcgcaaa ccaactctaa aatttaacac cgacggactt tatgttgacg tggaatttcc
     2221 gggatatccc ccggaagttt ccggaaccag cccgttgctg gctcctggtt ctggtgcgat
     2281 gttcaaaagt ttgtgggatg ttgagtcacg catgacggac tacgacaaaa agctgggcat
     2341 cgagcagcat gttgtcttac cgcaattttc cggctggtgg tcgtatctca ttgaagctga
     2401 attggctgct cgaatggcgc ggtcgcacaa cgaagctttg ctccgcgtca tgagagagta
     2461 cccgggacgc atcttgggtg tggctctggt cgcgctgcag gacgttgccg gctcgatccg
     2521 cgaaacggag tgggcgcggg agaatggctt ttgcgcggtt gtattggata aagtttttcc
     2581 agtgaaagac catccttacg gggagccctt gggaagtcac cgggaactgt ggccgttttt
     2641 caagcgcgcc gaagaattgt ccatgccgat ctatcttcac aacgttcaac atggacaccg
     2701 catcagcaac ttctcgctgt ttcagagaga cggactgtac gttttcgctc ctgaagaagg
     2761 acagatgagt ttggtttcgc tgattaccag cgggctgctg gatgaatacc cgcggttaca
     2821 atttatttat accgaagctg gaacatcgtt tattaagccg ttagtggtgc ggctcgataa
     2881 gcttctggag gcgcctccca tcgattacgc cgaccaggaa gaaccactgg gcaaccgtcc
     2941 tttgaccaaa gtaggggaaa aattgcgaag agcaagggcc ctgaatccgg cccatatttt
     3001 tctcgagaaa aataaaaaac ctgccagtca ttactttaag aacaattttt attttaccat
     3061 cgagaccgaa gagccggagt tgccggaggc gattgaattt ttgggagctg ggcggtttct
     3121 ttttgccacg gactatcccc acgatgatcc gggcggaaga atgaaatttc aggatgtaga
     3181 gctactgaga aacaatccaa agatttcaga atcggacaag gagctgattc gctggcgcaa
     3241 cgcggaacga ctgtttcatt tggctctata accggccatt tgcaaaggtc atttatggcg
     3301 ataccggagc gttacaatac gggttacaac attggcgttg gcggtgcggt ggtgcgcggc
     3361 gggcagctgc tgctggtgcg acgcgcgtcg cgccatgggc acggcaactg gcagttgccc
     3421 ggaggtttca tcgagccgga tgaaactatc gagcaagccg tggttcgtga ggtacaggag
     3481 gaagcgggag tcaagggcga agtggaagcc gtccttgggc tccgtagtcg ttacgatccc
     3541 gatagtggca acgggattta catcgttctg ctcttgaggc ccgtgagcgg cgagcccacg
     3601 gcggacggtc acgaagtcga tcatgccgct tactttaccc tggaacagat ccgcgatctg
     3661 aacccgctgc ctccggtcaa ctgggaaatc gcccagcgtg tgttggaccc cactcggcgc
     3721 cttctgctgc ccaagccgtt aactaatctc aacggcgcaa aattcacctt gttcgtgggc
     3781 tagtaattgg cgcgtcggtc tgtccttctc cggtctcatc cacgttgaaa gttgaacctg
     3841 caattcttat cggaggctct tctaagaatc tagcggcttg aaggttggga aggtaccgga
     3901 ttcccggaag gcaacggagg gagaatcgtt actttgatcc gaagttggtc cgaggatttg
     3961 gcgatggcgt cattgttacg gtcgggtgtg gaagattcca cttcgatatt ccccagatct
     4021 gcacgttctt tcttgaatag ctcgtattgg ttacgcgcaa tagtgaccca cgcagtctgt
     4081 gactgacccg tttggattgc gcgctgccgg gcctgatcga ggttttttac ttcctcctgc
     4141 gaagtcaacg atcgtcgtac agcctgggcc ccgcctgatg ccaatcgatc tcccatggct
     4201 ttgtcatcgc gcaccggttc ttttaggcgg atcgccaact cgtgatcagc agggactgcc
     4261 acgccgacca cagtgccgga tctaagagtg tccaaggatg aggccgcgct tctttctcgc
     4321 gcttcgggag aaaatgatga ctttgccgca ctaactggtg gtggcgttgg cgttaaggcc
     4381 tctttcgatt gtgcttcccg tgaagatgaa gattgttctc ggcgggggaa ggcagcctcg
     4441 ctcgccgctg aagatttctc ttggagcggg acaggactga aggtagccgg cgagcggacc
     4501 tggtcttgcg gacgcgcagt ttccggagca ttatcctgtt cttctggctt ggggcgtgat
     4561 tgaaggtctt tcacttgtat tgaatccttg aattcttgaa gccgcggttt ggtttcctcg
     4621 gcgaccccct tcgtcttaga ggtgggcgcc ggcgcgacgg atggcgccaa tctgtcagtt
     4681 tcattctttt ctctaaagga gctctcaggt tgaatgccca ctattgattc tcgctgcagc
     4741 ggttccttct ggtaaatgt
//
LOCUS       DASSWZ010000081         7942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_214380, whole genome shotgun sequence.
ACCESSION   DASSWZ010000081 DASSWZ010000000
VERSION     DASSWZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214380"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFN58_02085"
     CDS             <1..463
                     /locus_tag="VFN58_02085"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET8916808.1"
                     /translation="TLHKLGLARKLQAAITVQYPDERAPYPDTFRGRHRLTLKVDGSV
                     QCTACFLCATACPAECIYIEAGEYPDNPVEKYPIRYEIDTLRCIYCGFCVEACPCDAI
                     RMDTGTHPGNLGFSRKDFVENKEVLMERSRELAAKGKEGLYEEYVKPYRHV"
     gene            502..2430
                     /gene="thiC"
                     /locus_tag="VFN58_02090"
     CDS             502..2430
                     /gene="thiC"
                     /locus_tag="VFN58_02090"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET8916809.1"
                     /translation="MSNSDNRNESKPSGITTQPLPASQKAYVHSHEHPDIGVPMRAIS
                     LSGDHAGNGHANAPVMVYDTSGPYTDPSVETDIRAGLRPLRSEWIKARGDVEEIPRSY
                     ASANGKNGSHTERFPETSRRPVLRAKAGCNVSQMHYARKGIITPEMEYIAIRENIGRQ
                     AALENGANGNPRTTTGNSFGASIPKFVTAEFVRDEVARGRAIIPANINHPESEPMIIG
                     RNFLVKINANIGNSAVASSVEEEVEKMIWATRWGSDTVMDLSTGDNIHETREWILRNS
                     PVPIGTVPIYQALEKVDGKAEELTWEIYRDTLIEQAEQGVSYFTIHAGVLLRYVPLTA
                     KRITGIVSRGGSILAKWCLAHHKENFLYTHFEDICEIMKAYDISFSLGDGLRPGCIAD
                     ANDEAQFGELETLGELTEIAWKHDVQVMIEGPGHVPMHLIHENMTKQLEACHEAPFYT
                     LGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKKDVKDGVIAYKI
                     AAHAADLAKGHPGAQLRDDALSRARFEFRWEDQFNLSLDPDTAREFHDETLPAQGAKV
                     AHFCSMCGPHFCSMKITQDVRDYAAQKGIEEQAALAVGMEEKAEEFKKSGAKIYQEVT
                     SSSGGGSEPPAYRAAPKE"
     gene            2569..3345
                     /locus_tag="VFN58_02095"
     CDS             2569..3345
                     /locus_tag="VFN58_02095"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8916810.1"
                     /translation="MALPRLALSMGVQSNDTKDKLKTAGGRYGRKGSLVKFSLIFLLL
                     FTVPVAWRWTPLNEWINFSTVFDWQQSIRNHPAAFYLVVGVYVLGGLVLFPVTILNLA
                     TVFTFGPIQGNGYALAGWLASASMGYAIGHGIGREMVQKLARPWLDRLIRSVDRHGFL
                     TVLTLRVFPVAPFTLVNVFIGASGIRFRDFFLASLIGRIPGMILLSLAGVQVENLLRR
                     PDAISLILLGLTLVLLPLALNGLFRRALISNQRKKHSSQS"
     gene            complement(3398..4480)
                     /locus_tag="VFN58_02100"
     CDS             complement(3398..4480)
                     /locus_tag="VFN58_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916811.1"
                     /translation="MAILTHVTARLSSGKHKMVARRTQIYTFLFLVLMSFQSSCRTGP
                     VAPTPQRAPEAEKAEPTGKADAQTKSESTRIDQERSKDPKLSGAAKPTARLPESIVGA
                     PGKELASAKIAYDTVMMERAPHGSIHAGWQYRKDRQSQIVGFEFSNRGGNRILPPRYD
                     IEKNLLFARDFQFRFDDRARQDIQLFISDWAPSRDKQFRLSELMNSVLHFFPRNYLPA
                     ILGSDGRYVVTLPTGEQVEFDAKTREILGGVFSETAVDLNPDRAARKFPGVNYLGKGV
                     VVRANARGTDPRIGSTATITTGTPAQDCDKGAACNQCQVPSKELWHQNGPVRFRFPTD
                     QEFDRYLVSRCGFGIPGPIIVALPRE"
     gene            complement(4664..5128)
                     /locus_tag="VFN58_02105"
     CDS             complement(4664..5128)
                     /locus_tag="VFN58_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8916812.1"
                     /translation="MAKKAKAVPRGYRTVTPYLIIKGAASAIDYYKQAFKAKVLMCLS
                     EPNGKVGHAEIKIGDSRIMLADEYPETGSRGPQSFGGSPVSILLYVDDCDDIFNRAIA
                     RGAKVVKPMSDQFYGDRAGTLEDPFGHTWTVATHKEDLTSEEILQRAAARSG"
     gene            5381..5653
                     /locus_tag="VFN58_02110"
     CDS             5381..5653
                     /locus_tag="VFN58_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916813.1"
                     /translation="MMNFVETVKIDDPRLDESFSFLFVYRKGDVVVSRENRALRGQIN
                     DGVYVGELPNRASRALNPKGKTVYEIKFPDEVLQIFDEKEIEKVSE"
     gene            complement(5673..6662)
                     /locus_tag="VFN58_02115"
     CDS             complement(5673..6662)
                     /locus_tag="VFN58_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916814.1"
                     /translation="MLKAYSRVFVFVFSAMPVILGAQERLLVSYAGFGGFQAPAWAAK
                     DLGLFTKYGLSTELVMIPGSARGTQALLGGSTHFGQIDGTALIAAINQGADLVLIAAS
                     LNKFPFSLVAQKTITKPADLVGKKIGIVALGGAHEVSMVLALKEWNIPRQSVTLLTSG
                     PAANRLVALSSGALDATLLAPPETGEASRMGLPTLAHMTDLKAAAFPMNVIATRRSFL
                     DKNRDVVKRFLQAYSEATYQFMTNKEKALAIYNQRMKQKNPAVVEETYQYFAATFSFP
                     PRVSHEGMRVAMEMIAQRTPGTKLDTNVDKYVDERLLDELEREGLFRKISGKS"
     gene            7161..7490
                     /locus_tag="VFN58_02120"
     CDS             7161..7490
                     /locus_tag="VFN58_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916815.1"
                     /translation="MVRPRPDVDRREIERTPAERPPDVRDSAKTEIIGTVERVDKIKN
                     EIQLRTTEAKVIVIKYDPATLVYSRERQVGIEALRLRDLILVRVAKTPQGEQYADLIR
                     LNDRKEQ"
     gene            7493..>7942
                     /locus_tag="VFN58_02125"
     CDS             7493..>7942
                     /locus_tag="VFN58_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916816.1"
                     /translation="MNTVVGIFKTRQAATAAGNLLKAAGFEGDKVIILAPDTPDPVVE
                     KTVPTEDAEQPGMGKAIGSVVGGAVGLGAGALIANLLLPGIGPVLAVTFGAAAGGLGG
                     AAAGAAAGGALENLLSIGVPKDEIFFYEDALRQGRSVVIGLSEDDDQI"
BASE COUNT         1803 a         2043 c         2279 g         1817 t
ORIGIN      
        1 cactttgcat aagctcggcc tagcccgcaa gctgcaagcg gcgattaccg tccagtatcc
       61 cgatgaaaga gcgccgtatc ctgatacctt ccggggtcgg caccgcttga ccttgaaagt
      121 tgacggctcg gttcaatgca cagcctgctt tttgtgcgcg actgcctgtc ctgctgagtg
      181 catttatatc gaggcgggtg agtatccgga caatcccgtt gaaaaatatc cgatacgcta
      241 cgagatcgac accctgcgct gcatctattg cggtttttgc gtcgaggcgt gtccctgcga
      301 tgcgattaga atggatacgg gaacgcatcc aggcaatctt ggctttagcc gtaaagattt
      361 tgtcgagaac aaagaagtgc tgatggaacg atcgcgtgaa ttggcggcga agggaaaaga
      421 agggctctac gaagaatacg tcaaacctta ccgtcatgtt taaccgcttt cgggaaagtc
      481 cgcatccaag gggggatcaa aatgtcgaac tccgataatc gaaacgaatc gaagcccagc
      541 ggaataacca ctcagccgct tccggcttcg caaaaggcgt atgtccatag tcatgaacat
      601 cccgacatcg gtgtcccgat gcgcgccatt agtttgtccg gcgatcacgc gggtaacggt
      661 catgcgaatg ctccggttat ggtttacgac acttccgggc cgtacaccga cccgagcgta
      721 gaaaccgata tccgtgcggg gctcaggccg ctgcgctcgg agtggatcaa agctcgcggc
      781 gatgtggagg aaatccctcg cagctacgcc agcgccaatg gcaagaacgg ttcgcacacc
      841 gagcgattcc ctgaaacttc ccggcgtccg gttctgcgcg ccaaggccgg gtgtaacgtt
      901 tcccagatgc attacgcgag aaaaggcatc atcaccccgg agatggagta catcgctatt
      961 cgcgagaaca ttggccgcca ggcggctctg gaaaatggcg ccaatggcaa cccgcgaacc
     1021 accaccggaa acagtttcgg cgcatcgatt cccaagttcg tgacggcgga atttgtcagg
     1081 gatgaagtgg cgcgcgggcg ggcgattatc cccgccaata tcaaccaccc ggaaagcgag
     1141 ccgatgatca tcggtcgcaa cttcctggtg aagatcaatg ccaatatcgg caactctgcc
     1201 gtcgcatcat cggtggaaga agaggtcgag aagatgatct gggccacgcg ttggggctcg
     1261 gatacggtca tggacctttc gacgggcgat aatatccacg agacgagaga gtggatcctg
     1321 cgcaattcgc cggtgcccat cggaaccgtg ccgatttatc aagccctgga aaaggtcgac
     1381 ggcaaggctg aagagctaac gtgggagatc taccgagata cgctgatcga gcaggccgag
     1441 cagggggttt cgtactttac gattcacgcc ggcgtgttgc tgcgctatgt gccgttgacg
     1501 gctaaacgga tcacgggcat cgtttctcgc ggtggctcga tcctggcaaa atggtgcctc
     1561 gcccatcaca aggagaattt cctctacacc catttcgagg acatctgcga gatcatgaag
     1621 gcctacgaca tctcattctc tctgggtgac gggctgcggc ctggatgcat cgccgacgcc
     1681 aatgatgaag cgcaattcgg cgagctggaa accctcggtg agctgaccga gatcgcctgg
     1741 aagcacgacg tgcaggtgat gatcgaaggg ccggggcacg taccgatgca cttgattcac
     1801 gagaacatga ccaaacagtt ggaagcgtgc catgaggcgc cgttctacac cctgggcccg
     1861 ctgaccaccg acatcgcgcc cggttacgat cacatcacca gcgccatcgg tgcggcgatg
     1921 attggttggt acggcacggc catgctttgt tacgtaacac ccaaggaaca cctcggcctg
     1981 ccggacaaga aagacgtgaa ggacggagtg atcgcctaca agatcgcggc ccacgctgcg
     2041 gatttggcga agggtcaccc gggcgcgcaa ctgcgcgacg acgctctaag ccgcgcgcgc
     2101 ttcgaatttc gctgggagga tcagttcaac ctgagcttgg atcccgacac ggcgcgcgaa
     2161 tttcacgacg agacgttgcc ggcgcagggg gccaaagtgg ctcacttctg ctcgatgtgc
     2221 gggccccatt tctgctccat gaaaatcact caagacgtgc gcgactacgc ggctcaaaag
     2281 ggtatcgagg agcaggcggc attggcagtc ggcatggagg aaaaggccga ggagttcaaa
     2341 aagagcggcg caaagattta tcaggaagtg acttcttcga gtggcggcgg gagcgaaccg
     2401 ccagcctatc gagccgcgcc gaaagagtag tagcaaaacg gtctaacgtt caaagaggcc
     2461 ttacttcgta gaatcgaagt aaggcctctt ttagtcccga gaaacaattt ctaaacacca
     2521 gactcagaac tcaatcgatg cttaaggtcg ggcataggac cttgcttgat ggctttgccc
     2581 cggttagctt tgagtatggg agttcagtcg aacgatacca aggacaagct gaaaacagcc
     2641 ggcggtagat acggacgtaa agggtcttta gtcaaatttt cgctgatttt ccttctgctc
     2701 ttcacggtcc cggtggcctg gcgctggacg cctctcaacg agtggatcaa cttcagcacg
     2761 gtcttcgact ggcaacaatc gataaggaac cacccggcgg cgttttatct cgttgtcggc
     2821 gtctatgtgt taggcggtct ggttcttttc cccgtaacga tcctcaatct cgcgacggtt
     2881 ttcactttcg ggccgatcca gggtaatggt tacgcgctcg ccggatggct cgccagcgcg
     2941 tcaatggggt atgctattgg gcacgggatc ggccgcgaga tggtgcaaaa actggctcgg
     3001 ccgtggcttg accgcctgat tcggtcggtc gaccgccatg gctttctcac cgtgcttacc
     3061 ttgcgcgtgt ttcccgttgc tccgtttact ctggtgaatg tttttatcgg cgcgtcggga
     3121 attcgttttc gggatttttt tctggcgagt ctgatcggca gaattccggg gatgatcctg
     3181 ttgagtttgg ccggtgtcca agtcgaaaat cttttgcgcc gcccggatgc gataagtctc
     3241 atcctgctgg ggctcacgct ggtgctgctt cctctcgcgt taaatgggtt attcaggcgc
     3301 gccctgataa gcaaccagcg gaaaaaacat tcctctcaat cttaaaagac tgccgccggg
     3361 agcggcaaca gcgattgccg aggttgtggc tgttgaacta ctcgcgcggt agggcaacta
     3421 tgatggggcc gggtatgccg aatccgcagc gtgataccag gtagcgatca aattcctggt
     3481 ccgtcggaaa cctgaagcga accgggccat tttgatgcca aagttccttg gacggaactt
     3541 ggcattggtt gcaagcggct cctttgtcac aatcctgagc cggcgttccg gtagtgatcg
     3601 tagctgtact cccgattcgt gggtcagtgc cccgcgcatt agcgcgaaca accacgcctt
     3661 tgccgagata attgacgccg ggaaattttc gcgccgctcg atcgggattg aggtccaccg
     3721 cggtttcgga aaatacaccg cctaggatct cgcgtgtttt ggcgtcaaat tccacttgct
     3781 caccggttgg aagcgtgaca acgtaacggc cgtccgagcc aagaatcgcg ggcagataat
     3841 ttcttggaaa aaaatgcagg acgctgttca tgagctcgct aagtctaaac tgtttgtccc
     3901 gggatggggc ccaatctgaa ataaatagct gaatatcctg cctcgcccgg tcgtcaaagc
     3961 gaaactggaa atccctggcg aaaagcaaat tcttttcgat gtcatagcgg ggaggaagta
     4021 tccggttacc gccgcggttg gaaaactcaa aaccgacgat ctggctttgt cgatctttcc
     4081 ggtattgcca tcctgcgtgg atcgatccgt ggggcgctct ttccatcatc actgtatcgt
     4141 aggcaatttt ggccgaagcc aattcttttc ccggcgcgcc aacgatggat tcgggtaagc
     4201 gtgcggtagg cttggctgca cctgaaagct ttggatcctt actgcgttcc tggtcaattc
     4261 tagttgattc cgatttggtc tgcgcatccg ctttgccggt aggttccgct ttctcggctt
     4321 caggggcacg ttgtggtgtc ggcgcaacgg gacctgtcct gcagctcgat tggaaggaca
     4381 ttaatacgag gaacaaaaat gtataaattt gagtccgccg ggcaaccatt ttgtgcttcc
     4441 cggacgatag acgggcggtc acgtgtgtta aaatcgccat attgcaagct atctgtattc
     4501 cgctttaccg ggcttgatca ctaaaggcaa cgtccatgcc aaggccgggg tgagttttcc
     4561 ccttggtgct cagaaaattt accgaagaac tttcgacagc tgccaaggcc aggcttcggc
     4621 atcgaggagg ctatcttggc ggtttggacg ctggttgcac gctttagccg gatctcgcag
     4681 cagcacgttg caatatttct tccgacgtga ggtcttcctt gtgggtagct actgtccaag
     4741 tgtgtccgaa tggatcctcg agagttccgg cgcgatcgcc gtaaaactga tcgctcattg
     4801 gtttgacgac tttggcccct ctagcgatgg cccggttgaa tatgtcatcg caatcatcca
     4861 cgtagagaag aatactcacc ggtgagccgc cgaacgattg ggggccgcgc gagcccgtct
     4921 ctgggtattc gtcggccagc atgatacgag aatcgccgat tttgatttca gcgtgcccta
     4981 ccttgccgtt gggttcggat agacacatta acaccttcgc cttgaaggcc tgtttgtagt
     5041 agtcgattgc actcgctgca cccttgatga tcaagtaagg tgtcactgtt cgatagccgc
     5101 gcggaacggc tttcgccttt ttcgccatga tccgtctcct tcttaaaggc gtttagtttt
     5161 tgtgcgttac aactgtggaa aacgcggttt ttctcgcaag acgtatgcca cgacagcatc
     5221 atggaatatc gccatcgcat cgaccgacat ttaggtctct ccgaaaagtt ctgtatgaat
     5281 tctcctgcat cataggtgaa aagaactcaa aaaaagggtc ggacgtgtgg agatttttag
     5341 tccacgggtg aacggccttg aaaaaaagag gaaaagagcg atgatgaatt tcgtggaaac
     5401 cgtgaaaata gacgaccccc ggctggacga gagtttttca tttcttttcg tatatagaaa
     5461 aggcgacgtt gtcgtcagca gagagaatcg agcgctacga ggacagatca atgacggtgt
     5521 ctatgtcgga gagcttccca accgagcgtc gagagctctt aatccgaagg gcaagacggt
     5581 gtacgaaatc aagtttccgg atgaggtgct gcaaatcttc gatgaaaaag agatcgaaaa
     5641 agtttcggag tgaagggtat ggaaacagcc ttttagctct taccgctaat ttttctgaat
     5701 agtccttctc tctccagttc gtctagcagc cgttcatcga catacttgtc tacgtttgta
     5761 tcgagcttgg ttccgggggt gcgctgagca atcatctcca tggcgacgcg catgccttcg
     5821 tgggaaaccc ttgggggaaa agagaaagta gcagcgaaat attggtaggt ctcttctacg
     5881 acggcggggt ttttttgttt catccgctgg ttatagatcg ccagggcttt ttctttgttg
     5941 gtcatgaact gatacgtggc ctcgctatag gcctgcaaaa agcgtttgac gacgtcacga
     6001 ttcttgtcga gaaaggagcg gcgcgtggcg atgacgttca tggggaaggc ggcggccttg
     6061 agatcggtca tgtgagcgag ggtaggcaat cccatgcgcg atgcctctcc tgtttccggc
     6121 ggggcgagta acgtcgcatc cagggcaccg ctggaaaggg cgaccagacg gttggctgcc
     6181 ggaccgctgg taagcaaagt tacggattgc cgcgggatat tccactcttt gagcgccagg
     6241 accatggaaa cttcgtgggc gccgccaagg gcgacgatac cgattttttt gccgaccaaa
     6301 tcggccggct tggtgatagt cttttgcgct accaggctga aggggaattt gttaagcgac
     6361 gcagcaatca gcacgagatc ggctccctga ttgatggcgg cgatcaaggc ggtcccgtcg
     6421 atctgcccga agtgggtgct tccacccaat agggcctggg ttccgcgggc tgatccggga
     6481 atcatgacca actcggtgct caagccatac ttggtaaaaa gccccaggtc tttagcagcc
     6541 cacgcaggtg cctggaatcc gccgaagccg gcatagctca ctagcaagcg ctcttgtgcc
     6601 ccgagaatta ccggcatcgc actaaaaaca aaaacaaaca cgcggctgta agcctttaac
     6661 atctcagcct cctctgtcct aggatctgct attcaatccg agccgctgcg atttaacctt
     6721 gatccaacag aaatagtcaa gccgcttcgc ggttcgatgg cgcaggcgtt gtgagttttc
     6781 ttcgcagtct ttgtaggaaa attcctatca gcgagccctt agcattgcgg aaagtctcta
     6841 tcaatcttca tcttattcaa agaaagacaa tggcataggc cttgcggaat taacgggacg
     6901 cgacatcgta aaagtatcaa accaaggagg tggcgtcatg acagagcaac ctaaccagtt
     6961 caagtgtcat agttgtggcc ggtcgtttag ctctgaggac cagttgcgtg aacatgagaa
     7021 aaattgttca ggtccccgtc cgcacggagg ccagtaacca aatgtttttt agatttataa
     7081 caaggactca ggcggtagct taccggtctc ggattatttt gctgggtttt atcttcaccg
     7141 ctgtgggttg tgagagcatc gtggttcggc cacgaccgga cgtggacaga cgggaaattg
     7201 agcggacccc agccgagcgg cctccggatg tgcgcgacag cgcaaagact gaaattatcg
     7261 gtaccgtaga acgggtcgac aaaatcaaga atgaaattca actgcggact acggaagcga
     7321 aagtaatcgt catcaagtat gatccggcta ctcttgtcta cagtagagag cgccaagtcg
     7381 gcatcgaagc cttgcgtcta cgtgatctca tcctagttcg ggtggcgaaa actccccaag
     7441 gggagcaata cgcggacctg atccggctaa acgacagaaa ggagcaataa ttgtgaacac
     7501 agtcgtcggt atttttaaga ctcggcaagc agccactgct gccgggaatt tattgaaggc
     7561 agcaggtttc gaaggtgaca aggtaattat tttggcacca gacacccctg acccggttgt
     7621 cgagaagaca gtgccaacgg aggacgcaga acaaccgggc atgggaaaag ccatcggtag
     7681 cgttgttggg ggagcggttg gtctcggcgc gggagctctg atcgctaatc ttcttttgcc
     7741 tgggattggt ccggtcttgg cggtgacatt cggagccgcg gccggcgggt tgggtggcgc
     7801 cgcagccgga gcggcggcag gtggcgcact ggagaatctt ctgtccatcg gcgtaccgaa
     7861 ggacgagatc tttttttatg aggatgcgct tcgacaggga cggtccgtcg tcatcggact
     7921 ttcggaggac gacgatcaaa tc
//
LOCUS       DASSWZ010000082         5635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_313603, whole genome shotgun sequence.
ACCESSION   DASSWZ010000082 DASSWZ010000000
VERSION     DASSWZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313603"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFN58_02130"
     CDS             complement(<1..278)
                     /locus_tag="VFN58_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HET8916817.1"
                     /translation="MSDRLTWDQYFMTITQQVAERSTCARAKVGAVIVRDKNILATGY
                     NGAPAGMPHCTDVGCLIYQSKTPNGETEENCFRTIHAEMNAIAQAAKNG"
     gene            complement(308..1090)
                     /locus_tag="VFN58_02135"
     CDS             complement(308..1090)
                     /locus_tag="VFN58_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET8916818.1"
                     /translation="MLITLTTDFGYTDPFVGIMKGVICGINPHARLVDLSHGIPAQNI
                     MAAALVLRHSAQYFPRGTIHTVVVDPGVGSARRPLLVELEGSYFIGPDNGVLSLVMEA
                     KTPTRIVHLSNPSYQLQPTSATFHGRDIFAPTAAYLSRGIAPEAFGETTDDFVKLIWP
                     TVLKLETAISGEIVYIDSFGNLFTNIGADDLKGLPGHRLRITLRDLSILGLAANYTAV
                     EQDKYVALVNSWGLLEIAIYKGSAQKHSGAIIGDKVQISWVS"
     gene            complement(1237..2007)
                     /locus_tag="VFN58_02140"
     CDS             complement(1237..2007)
                     /locus_tag="VFN58_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8916819.1"
                     /translation="MVSRSLGQTGLIVSRIGLGTTKLGRNTDVKYPKAFALPSDKQVG
                     ELLDTALRSGVNLIDTAPAYGASEARLGAFVGNYRDRLILSTKCGEQYRNGSSVYEFS
                     TGAITASIEASLRYLNVDHVDILLLHSDGRDIEILTRTDGLEALMRLKQSGKTRAIGI
                     SAKTPDGIHEACRTLDVIMAPFSQKDPSLAEALRQAHQAGLGILAIKGLSSGYLEARP
                     AIEFVLHQEFIDALVLGTTEPAHLSEAVSIAENMTIAS"
     gene            complement(1989..3164)
                     /locus_tag="VFN58_02145"
     CDS             complement(1989..3164)
                     /locus_tag="VFN58_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8916820.1"
                     /translation="MRLDLLIFGGGAAGLWCLDRFRRAGYLATLLESTALGRGQTIQA
                     QGIIHGGGKYALRGVRDFTAVRSTKAMPERWRRSLAGEIEPDLRGAQVLSDRCHLWLP
                     RGSMAARALSWGLMPLLAKGGLLSTPPQQLSESMWPEALRGSALVVYALAEPVVATGS
                     LLDALARPHRKWIFSYNVSDLQFAGGQVEIAGALFQPRSIVFAAGEGNAELMRRAGIR
                     GDLMQRRPLKMVLLRGTTLPVLFGHCIIRGKTQVTITTPTQGIWQVGGEIAEQLARQE
                     HLEDGRQAALREVRGCLPGLDFSGVEIAIYSATRAEAKTAEQKRPSGVHVSRAAAGIV
                     VGWPTKLSMAPILAEEVFALVHGELKQPGDYEEPSVPWPTPPVAPYPWEEVEWFPAR"
     gene            complement(3308..4711)
                     /locus_tag="VFN58_02150"
     CDS             complement(3308..4711)
                     /locus_tag="VFN58_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET8916821.1"
                     /translation="MTQLIDTEYEGTALIADPIHRYIQFTVPLDKSELTEKALIDSPW
                     VQRLRYIYQLQSARWVYPSAEHSRFQHSLGAMHMAGEFAKHLYPSLKKVLGDECPSAP
                     FVEEYMRVTGLLHDVGHGPFGHFFDDNFLYAYDLTHEDLGQIIIQNHLGEIISQLRRS
                     PSGPFDPGEELRPEDIAFPIKKDGKEDHRKPKWVRFLEPLTSGIFTFDNLDYVSRDSY
                     MCGVAVGPVDRARLLHYTFFSPKGLTLHKAGNSALTMFLNTRLYLYTNVYYHRTTRAI
                     DLHLREIFPETMRVLFPGNPAEHMDEYLYLTDWSLLEEVSRWRRNKGELAALSKEWET
                     VLRREVKWKMAYDRTLSLNELRYGVALIQDADWEKRIRSALPTTARDLVFRVDMATQD
                     PRPENPFAMGKKQIHIFDPSTGEITKESLAELFEFIPSKVVQFRVFALNHEQDQLLSE
                     VAESTLKGQSPSAKTSV"
     gene            complement(4718..5143)
                     /locus_tag="VFN58_02155"
     CDS             complement(4718..5143)
                     /locus_tag="VFN58_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HET8916822.1"
                     /translation="MENQKRYRLTPRQSEIAVRVSGNSQADLFANSAFALFDVMTDIE
                     KVDIKDRVPLEVEGADRDDLMVNWMRELLYLYQGSGYLLKEFHIREAKDTVVKAEVCG
                     EKTDPDRHEINKEIIGIAYHQSRMQKTGDQWTAQLIFEI"
     gene            complement(5437..>5635)
                     /locus_tag="VFN58_02160"
     CDS             complement(5437..>5635)
                     /locus_tag="VFN58_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HET8916823.1"
                     /translation="ALPSVVAGLIDKPVIAVPTSVGYGASFNGLAALLAMLNSCASGI
                     TVVNIDNGFGAGFAASLINRV"
BASE COUNT         1224 a         1513 c         1543 g         1355 t
ORIGIN      
        1 ccgttctttg cagcttgggc gatggcattc atttcagcgt ggatggttct gaagcagttc
       61 tcctcggtct ctccgttagg agttttggat tgatagatga gacaaccgac atcagtgcag
      121 tggggcatac cggccggcgc gccgttgtag ccagtggcga ggatgttctt atcccggacg
      181 ataaccgccc cgaccttggc ccgggcacag gtggaacgct ccgccacctg ctgcgttatg
      241 gtcataaaat actgatccca ggttaatcga tcgctcatga atgacctccg tttggagctc
      301 caacgtctta cgaaacccag ctgatctgga ctttgtcgcc gatgattgca ccgctatgtt
      361 tttgtgcact ccccttgtaa atagcaatct ccaatagtcc ccagctgttc actaacgcaa
      421 catacttgtc ttgctcaacc gcggtataat ttgccgccag gccgagaatt gagagatcgc
      481 gaagcgtgat ccggagtcta tgtccaggta ggccttttag atcatccgcg ccgatattcg
      541 taaacaggtt accgaaactg tcgatgtaca caatctcgcc gcttatggcc gtctcgagct
      601 tgagaaccgt gggccagatc agtttaacga aatcgtctgt ggtttcgcca aaagcctctg
      661 gcgcaatgcc gcgtgaaaga taggcagccg ttggagcgaa aatatctcgt ccgtgaaagg
      721 tcgcactggt gggctgcagt tggtaactcg gattcgatag atgaacgatg cgtgtaggcg
      781 tctttgcttc catgacaaga ctcaacactc cattgtccgg ccctatgaaa tagcttccct
      841 cgagttctac aagcagaggc cggcgtgcac tgcccacacc tgggtccact accacggtat
      901 ggatcgtccc gcgtggaaag tattgtgccg agtggcgtag gaccagagcg gcagccatga
      961 tgttttgcgc gggaataccg tgactgaggt ccactagtcg agcgtgcgga ttgattccac
     1021 agatgacgcc tttcatgatc ccgacaaaag ggtctgtgta gccgaaatcg gtggtgagcg
     1081 ttatgagcat cgcataaccc gtgagagagg tgaaatgaat ctaacggaaa cgaaagtaca
     1141 ggtaaaggtt agacccttca ccttccaagg aaaagctgta ggaaggattt gccgatgagc
     1201 aaattccagc tgggtttctg gctcgaaacg ctcagtttag ctggctatcg tcatgttttc
     1261 cgctatcgaa accgcttcgc tgaggtgtgc cggctcggtg gttcccaaaa caagcgcatc
     1321 gataaattcc tggtggagta caaactcaat ggcggggcgt gcttcgaggt aaccactgga
     1381 gagtccttta atggcaagga taccgagacc ggcttgatgc gcttgtcgca gggcttccgc
     1441 tagacttgga tctttctggc tgaagggagc catgatcaca tcaagcgttc ggcaagcttc
     1501 gtggattcca tccggagtct ttgccgagat acctattgcc cttgtcttgc cgctttgttt
     1561 aagacgcata agcgcttcga gcccgtcggt ccgggtgaga atttcaatgt cccgcccgtc
     1621 ggagtgtaac aagaggatgt cgacatgatc gacattgaga tacctgagac ttgcctctat
     1681 tgaagccgtg atcgcgcccg tggaaaactc ataaactgaa ctaccgttac ggtattgttc
     1741 gccgcatttg gtgctgagga tgagacggtc gcggtaattc ccgacgaagg cccccagtcg
     1801 cgcctcgctt gcaccatagg cgggcgcagt gtcgattaaa ttcacacccg atcttaaagc
     1861 ggtgtcgagc agctcaccga cctgtttgtc cgaggggagc gcaaaagctt tggggtactt
     1921 cacgtcggtg tttctcccca gtttggttgt tccaagacca atgcggctta cgatcaatcc
     1981 cgtttgccct agcgagcggg aaaccattcg acctcctccc agggatatgg cgcaacaggg
     2041 ggcgttggcc agggtaccga tggctcctcg tagtcccccg gttgtttgag ctccccgtgc
     2101 accaaggcga agacttcttc ggccagaatc ggtgccatcg agagtttggt gggccaaccg
     2161 accacaatgc cggcagcagc gcgcgagacg tggacaccac ttgggcgctt ctgctctgct
     2221 gtttttgctt ccgcccgagt cgctgagtaa atagcgatct cgacgcccga gaaatccaac
     2281 ccaggtaggc agccccggac ttcgcgcaag gctgcttgtc gcccgtcttc taggtgttct
     2341 tgccgtgcca attgctccgc aatctcgccc ccgacctgcc agatgccctg agtcggcgtc
     2401 gtgattgtaa cctgtgtctt gccgcgtatg atgcaatggc caaacagcac cggcagagtc
     2461 gtgcccctga ggagtaccat cttaagcggc cggcgctgca tgaggtcgcc acggatccct
     2521 gcccttcgca tgagttcggc gttgccctcg cctgccgcga acacgatcga gcgtggctgg
     2581 aaaagcgcgc ctgcgatttc cacctgtccg ccggcgaatt ggaggtcgga aacattgtaa
     2641 gagaagatcc acttgcggtg agggcgcgcc agcgcgtcaa gaagcgagcc cgttgcaaca
     2701 accggctccg ccaacgcata gacaacaagg gccgaacctc gaagcgcctc cggccacatt
     2761 gattccgaaa gctgttgggg tggtgttgac agtagtcccc cttttgcaag tagcggcatc
     2821 aatccccacg acagcgctcg tgccgccatt gaccctcgcg gcagccaaag gtggcatctg
     2881 tctgaaagaa cctgcgcgcc gcgcagatcg ggttctattt ccccggccag acttcgccgc
     2941 cagcgctctg gcattgcttt tgtagaccgc acggccgtga aatcacggac accgcgcagt
     3001 gcatactttc ctccgccgtg gatgattccc tgtgcctgaa tcgtctggcc gcgcccgaga
     3061 gccgtcgatt ccaggagagt tgcaagatag cctgcgcgac ggaaacggtc taaacaccag
     3121 agtccggcag cccctccccc gaagatgaga agatctagcc gcatctgtca gagcagtcga
     3181 acactgacgc gattggcagc cgggaaaagc ttgtggatcc gtgcgcggag gtgactcgat
     3241 gaggtacacg ggaaataggc gacgaaatga agcatcctgt ccgcttttcc tagtataccg
     3301 tctgccgtca aacgctggtt tttgcggacg gagactgtcc cttcagcgtg ctctccgcaa
     3361 cctcggacaa tagctgatcc tgttcgtggt tcagggcaaa aactcggaat tggacaacct
     3421 tggacgggat aaattcgaaa agctcggcca gagactcttt ggtaatctca ccggtggaag
     3481 ggtcgaagat gtgaatttgc ttctttccca tagcgaacgg gttttccggc cgcggatctt
     3541 gggttgccat gtctacccga aacactaggt ctcgcgcagt cgtcggtaac gctgagcgga
     3601 ttctcttttc ccagtcagcg tcttgtatca aagccactcc gtagcgaagc tcgttgagag
     3661 atagggtccg atcgtatgcc atcttccatt tgacttcgcg gcggaggacg gtttcccatt
     3721 ccttgcttaa tgccgcgagt tcgcccttgt ttcggcgcca acgggaaact tcttcgagca
     3781 atgaccagtc cgtcaggtaa agatactcgt ccatatgctc ggcagggttg cctggaaaaa
     3841 ggacacgcat ggtttcggga aaaatttctc ttaaatgtag atcgatggct cgcgtcgtac
     3901 ggtgataata aacattggtg tagagataga ggcgtgtgtt tagaaacatc gtcagcgcgc
     3961 tgttgcccgc cttatgcaag gtaaggccct tgggcgagaa aaacgtgtaa tggaggaggc
     4021 gtgcacgatc tacgggtccg acggcgacgc cgcacatgta ggagtctcgc gatacgtaat
     4081 ccagattgtc gaaggtaaag atgccgctcg tgagaggctc cagaaacctt acccatttcg
     4141 gtttgcgatg gtcttccttg ccatccttct taattgggaa cgcgatatct tcaggacgca
     4201 gctcctcgcc tggatcgaac gggccactgg gactgcgccg cagctggcta atgatctcac
     4261 ccagatggtt ttggatgatg atctgtccga gatcctcgtg ggttaaatcg tatgcgtaga
     4321 ggaaattgtc atcgaaaaaa tggccaaagg gtccatgtcc cacgtcgtgc agaagccctg
     4381 taactcgcat gtattcttca acaaagggag ctgacggaca ctcgtcgcca agcactttct
     4441 ttagagaagg ataaagatgt ttcgcgaatt ctcccgccat gtgcatggca ccaagagagt
     4501 gctgaaagcg gctatgctca gcagagggat agacccaacg ggcgctttgc aattgataaa
     4561 tatagcgcaa acgctgcacc cacggggagt cgatgagcgc tttttcggtt agttcggatt
     4621 tatccagtgg tacggtgaat tggatgtaac ggtggatggg gtccgcgatc agcgcagttc
     4681 cctcgtattc ggtgtcgatg agctgcgtca taagttttca gatttcaaag atcaactgtg
     4741 cggtccactg atcgccggtc ttttgcattc ggctttggtg gtaagcgatg ccaataatct
     4801 ctttattaat ctcgtggcga tcgggatcgg tcttctcgcc gcatacttcc gccttcacca
     4861 cggtgtcttt tgcctcccgg atgtgaaatt ccttcagcaa atagccgctt ccctgataga
     4921 gatataatag ctctcgcatc caattgacca tgaggtcgtc ccggtccgcc ccctctacct
     4981 ccagaggtac acgatccttt atatcaactt tctcgatatc agtcatgacg tcgaaaagtg
     5041 caaacgcgga attggcaaag agatcggctt gagaattgcc tgagaccctt acagcgatct
     5101 cagattgccg aggcgttaga cggtatctct tttggttctc catgattcat gagcctttgg
     5161 cgttcatcgc ggttgcttgg tgtttcttca cagccggcat ctgccagcga gcactgactg
     5221 tccttggtta attcgtggtc tgatcactgg gttggcgttg aattgacacg agagaacgtt
     5281 tccttgtgat atcggcgggt tggcgcatcc tctttagtgt tgttggagaa ggccatgctc
     5341 tgtccggatt ggagcagaga gtctgttatc tcttaagttc tcttctttgg tactttctct
     5401 aagtctctgg ctcgtgggca tatgcactga gcttatttat acccggttta tgaggctcgc
     5461 cgcaaatccc gctccaaacc cattatcgat attgaccacc gtaatgccag acgcgcatga
     5521 attaagcatt gccagaagcg cagcaagtcc gttgaaactc gctccatatc cgacactggt
     5581 cggtaccgca ataaccggct tgtcgattag tccggcaacc acactcggca acgcc
//
LOCUS       DASSWZ010000083         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_638952, whole genome shotgun sequence.
ACCESSION   DASSWZ010000083 DASSWZ010000000
VERSION     DASSWZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638952"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFN58_02165"
     CDS             complement(<1..710)
                     /locus_tag="VFN58_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8916824.1"
                     /translation="MAKPAPQVDNAMDNIPYYEKWQLEEGIPIVKTFFVQDLKKVELK
                     PWDRTGGKGAFINMEGAEGTTGAYVLEIAPGKQTSPQRHLYEDLIFVLKGRGATTIWN
                     DQGAKQTFEWQDGSLFALPLNSWYQHFNGQGDEPARFFSVNSMPIVFNLFHNADFIFD
                     TPRDFTDRFNGEAEYFSGKGKAYPGRVWDTNFIADARNFKLEDWKERGGGGKNVMLEM
                     ANGSMAAHISEFPVGTYK"
     gene            964..1179
                     /locus_tag="VFN58_02170"
     CDS             964..1179
                     /locus_tag="VFN58_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HET8916825.1"
                     /translation="MAINVEAPMVGKILRVEKKVGDQVEEDEVVVVMEAMKMEIPVVA
                     PANGVLKELKVTPGQAVEAEQVLAVIE"
     gene            1224..1370
                     /locus_tag="VFN58_02175"
     CDS             1224..1370
                     /locus_tag="VFN58_02175"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HET8916826.1"
                     /translation="MDIWTFGWTLALIGMAGTMLVLWCLSLLILLLKKLFPYQPEESA
                     SRKG"
     gene            1380..2525
                     /locus_tag="VFN58_02180"
     CDS             1380..2525
                     /locus_tag="VFN58_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HET8916827.1"
                     /translation="MESALISGVQGLLLGVTNLGVGHVIMLLIASLLLYLGISKGYEP
                     LLLVPIGFGAILVNIPLAEMMGDDGFLRYFYNAGVRTEVFPLLIFLGIGAMTDFQPLL
                     ENPKIILLGAAGQFGIFLTLLLALALGFDKLDAVAVAIIGACDGPTAIYVSSKFAPHL
                     LAAVSVAAYSYMSLVPLIQPPIMRFLTTEKERKIAMGVVRQPVSKTTKILFPIVVTIF
                     ASILAPKGAPLIGTVMLGNLMKESGVVDRLKRAAENEIANIVTLLLGLCIGATMEADQ
                     FLQAQTLLVLGLGFIAISLDTVVGVLFGKLMCLLTAGKINPLIGAAGISAFPMAARVV
                     QVEGQKYNKKNYLLMHAMSANAGGQIGSVIAAAVMLSVLQGMGIVGG"
     gene            2526..>2707
                     /locus_tag="VFN58_02185"
     CDS             2526..>2707
                     /locus_tag="VFN58_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA carboxyltransferase"
                     /protein_id="HET8916828.1"
                     /translation="MANTREDLEKKLAALEQRAADGDPKVAAKQRAEGKLTARERVHK
                     LLDPGSFVEEFMLAET"
BASE COUNT          611 a          720 c          719 g          657 t
ORIGIN      
        1 ttcttgtacg tgccaactgg gaattctgag atgtgagcgg ccatcgagcc attggccatc
       61 tccaacatca cgttctttcc gcctccgcca cgctctttcc aatcttcaag cttgaaattg
      121 cgagcgtcgg cgatgaaatt agtgtcccaa actcgacccg gataagcttt gcccttgccg
      181 ctgaagtatt cagcctcgcc gttgaaacga tccgtgaaat cacgcggggt atcgaaaatg
      241 aaatcggcat tgtgaaaaag attgaaaacg atcggcatgc tgttgacgga gaaaaatcgc
      301 gccggctcgt ccccctggcc attgaagtgc tggtaccagg agttgagcgg taaagcgaac
      361 agactgccgt cctgccattc aaaagtttgc ttcgctcctt gatcgttcca aatagtcgtc
      421 gccccgcgtc ccttgagaac aaaaatcagg tcttcataaa gatgtctctg agggcttgtt
      481 tgtttgcccg gagctatctc cagaacataa gcaccggttg ttccctccgc gccttccatg
      541 ttgatgaagg ctccttttcc tccggttctg tcccaaggtt tcagttcaac cttcttcaaa
      601 tcctggacaa aaaaggtctt aacgatgggt atcccttctt cgagctgcca tttctcatag
      661 taaggaatgt tgtccattgc gttatcgact tggggcgccg gtttggccat atttatcctc
      721 ccgagaaagc tattttgggt cataccgtat caaacctgca aaagcaagga agtagctggc
      781 gtgcaaccaa gcacgcgctc cgatttccca gcctttccgt gcattagatt gttgactgtg
      841 ccactagcgt tttccgagac gatttatcca ggcgagcatt gacaagcatt gcgaaaccct
      901 actaaatggc aatactcgaa tgacatagcc gaaacgatgc atcagtgaga aaggagcaga
      961 gcattggcaa taaacgtcga ggcgcccatg gtgggtaaga tcctgcgggt tgaaaaaaaa
     1021 gtcggtgatc aagtggagga agacgaagtc gtggtggtta tggaagcgat gaagatggaa
     1081 attcctgtcg tagcgcccgc taatggggtc ttgaaagaac tgaaagttac cccgggtcag
     1141 gccgtcgagg cggaacaagt tctcgctgtc attgagtaat cccccctttc tagcgaccat
     1201 caaactattt gtacggagac ccaatggaca tctggacgtt cggttggaca ctggcgctca
     1261 ttggcatggc aggaaccatg ctggtcttgt ggtgtttaag cctgctgatc ctgttgctga
     1321 agaagctctt tccttaccag cctgaggaat cggcatcacg gaaaggttaa cgcctagtca
     1381 tggagtcagc gctcatttca ggggttcaag gtcttctgct cggtgtcacc aatctcggcg
     1441 tgggtcatgt aatcatgctc ctcatcgcct cgctgctcct ttacctcggg ataagcaagg
     1501 ggtacgaacc cctgctgctt gtgcccatcg gcttcggcgc gattctggtg aacattcctt
     1561 tggcggagat gatgggagac gatggctttc ttcgttactt ctacaacgcc ggggtgcgca
     1621 cggaagtttt tcctctgctg atctttctcg ggatcggcgc catgaccgac ttccagccct
     1681 tgttagaaaa ccccaagatc attctcctcg gagccgcggg ccaatttggt atatttctga
     1741 ctctattgtt ggctttggcg ctcggctttg acaaactgga cgcggtggct gttgccatca
     1801 tcggcgcttg cgacgggccc acggctattt acgtgagctc gaaattcgcc ccccaccttc
     1861 tcgccgcagt ttcggttgcc gcctactcct acatgtcctt ggtcccgctc atccagccac
     1921 ccatcatgcg ctttttgaca accgaaaaag aacgaaaaat cgccatgggc gtagtgcggc
     1981 agcctgtgtc gaaaaccacg aagatccttt ttcccatcgt ggtcaccatt tttgcttcga
     2041 tcctcgctcc caagggcgct ccgttgatcg gcaccgtgat gctcggaaac ctgatgaaag
     2101 aaagcggcgt ggtcgaccgt ttgaaaaggg cggcggaaaa cgagattgct aatatcgtca
     2161 ctcttctgct cgggctttgt atcggcgcaa cgatggaagc agaccagttc ttgcaagctc
     2221 aaacactctt ggtcttgggc ctgggtttca tcgccatcag cctagatacg gttgtcggcg
     2281 tcctcttcgg caagctcatg tgtctgctga ctgcaggcaa gatcaatccg ctcattggcg
     2341 ccgcaggaat ctcagcgttt ccaatggcgg cgcgggtggt tcaagtcgag gggcaaaagt
     2401 acaacaagaa aaactatctc ctcatgcacg cgatgagcgc caatgccggc ggacagatcg
     2461 gctccgtgat tgccgccgct gtcatgcttt cagtgctcca gggaatgggg atagtgggtg
     2521 gataagtggc caacaccaga gaagacctag agaagaaact ggcggcgttg gagcaacgcg
     2581 ccgctgacgg cgatccgaaa gtagcggcaa aacagcgcgc cgaaggcaag ctgacggccc
     2641 gggagcgcgt ccataagctt ctcgaccctg gcagctttgt cgaagagttc atgctggcgg
     2701 aaacaca
//
LOCUS       DASSWZ010000084         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_44696, whole genome shotgun sequence.
ACCESSION   DASSWZ010000084 DASSWZ010000000
VERSION     DASSWZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44696"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFN58_02190"
     CDS             complement(<1..812)
                     /locus_tag="VFN58_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8916829.1"
                     /translation="MQKSSERISTASLTVLALIAFAANSVLCRLALGGSAIDPASYTA
                     VRLITGAITLWLITVFRRNGSLGKSGGNWISAAMLFLYAVTFSFAYVSLSAGTGALIL
                     FAAVQITMITVGLSTGERPEILEWLGLFIAICGLIYLVSPGITAPSILGSVLMASAGI
                     AWGIYSLRGRGASDPVGVTTDNFVRTVPLAVGLILLWLSTLTITPMGFLWAAVSGSIT
                     SGVGYVLWYAALPRLTATRAATVQLAPAPLAALGGVVVLSEAISLRLVISTVV"
     gene            complement(1054..1470)
                     /locus_tag="VFN58_02195"
     CDS             complement(1054..1470)
                     /locus_tag="VFN58_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET8916830.1"
                     /translation="MKQLAIVVRDDGYDRMLTPLTFAYVQAKAGVKVDMLFVLWAVRA
                     LTEEGARSLTIDGPHASEAEWLRQRMSAAGEPTEIHDYMKLLAATGNVKFYGCQIAAS
                     TFGVKPSDLMPEAEGIVDSNWFLNEKALKADHCQYF"
     gene            complement(1505..2089)
                     /locus_tag="VFN58_02200"
     CDS             complement(1505..2089)
                     /locus_tag="VFN58_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8916831.1"
                     /translation="MTTPTIKTVTPSSADQAVGTVVLAFSADPIARWFYPDPHQYLLH
                     LPSFVRAFAGKAFEHNSAYCVDGYLGAALWLPPDVHPDEKVLAALLQRTIPEENQQEI
                     FAFTERMNSSHPSEPHWYLPMIGVDPAKQGNGYGSALMKHALARCDSEGKLAYLESSS
                     PRSIPLYRRHGFELLGTIQVGSSPPLFPMMRKPR"
     gene            complement(2346..2408)
                     /locus_tag="VFN58_02205"
     CDS             complement(2346..2408)
                     /locus_tag="VFN58_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916832.1"
                     /translation="MVEIKRRYNPEKCISLESEH"
BASE COUNT          603 a          683 c          678 g          578 t
ORIGIN      
        1 accacagtcg atatcactag acgtagacta atcgcttcag ataaaacgac aactccgcca
       61 agcgctgcta gcggtgccgg tgctagctgg accgtggccg cccgtgtcgc tgtcaggcga
      121 ggcagcgccg cataccacag gacatagccg actcccgacg tgatggatcc ggatacggcc
      181 gcccagagaa aacccatcgg ggttatagtc agcgtcgata gccataacaa aatgagaccg
      241 acggccaagg gcactgtgcg cacaaaattg tccgtggtga cgccgacagg atcactggct
      301 ccgcgacctc taagagagta aatgccccac gcgattcctg cgctggccat aagaactgac
      361 ccgaggatcg atggggcggt gatcccggga gacaccaaat aaatcagacc acagatggcg
      421 atgaataacc ccaaccactc taatatctcc ggtctttcgc ccgtagagag gcccaccgtg
      481 atcattgtga tttgcaccgc ggcgaacaga atcaacgcgc cggttccagc gctcaagctt
      541 acgtaggcga aagaaaaggt cacggcatat agaaaaagca tcgcggcaga aatccagttg
      601 ccgccggatt ttccgagaga tccgttccgg cggaaaacgg tgattagcca caaagtaatc
      661 gcccctgtga tcagcctgac cgctgtgtaa cttgcaggat cgatggccga cccgccaaga
      721 gccaatcggc acaagaccga gttggcagca aaggcgataa gggcgagaac cgtaagagac
      781 gccgtggaga ttctttcaga cgatttttgc aactgattac cttagcaagc tcgattgccg
      841 atgcccatta ggctcttacc ttggcatgtg acaagcatac tccggtcttc gataagaatg
      901 aaagttccct tccgtttccg tgacccgatc gtaggtttgg ctacctgttg catcaaagcg
      961 cgttggcatc gttttcttac ttgattaagc tcggtcaaaa tttcagcacg gtgctgtcga
     1021 tttcaatcca gcgcaccagg gtattgagac ggatcagaag tattgacaat ggtctgcttt
     1081 gagcgccttt tcgttcaaga accaattgga gtcgacgatt ccctctgcct ccggcatgag
     1141 atccgacggc ttcacgccaa atgttgaggc ggctatctgg cacccgtaaa atttcacgtt
     1201 accggtggct gcaagcagct tcatatagtc atggatctcc gttggctcac cagcagcgct
     1261 catgcgttgg cgcaaccatt ccgcttcgga cgcatgcggg ccgtctattg tgagcgaccg
     1321 tgccccttct tctgtgagtg cccggacggc ccagagcacg aacaacatgt ccactttgac
     1381 gcctgccttg gcctggacat aggcgaacgt aagcggtgtg agcatcctgt cgtacccgtc
     1441 atcacgaact acgatagcga gctgtttcat aaagtctcct ttcaaattca attgggatca
     1501 cgagtcaccg cggtttccgc atcatcggaa acagcggagg tgacgaacca acctgaattg
     1561 tgccgagcaa ttcaaagccg tgccgccggt agagcgggat acttcttggg ctggacgatt
     1621 cgagataggc gagtttgccc tcgctatcgc accgtgcaag cgcatgtttc atcaacgcgg
     1681 aaccgtagcc gttgccttgt ttggccgggt ccactccaat catcggcaaa taccagtgag
     1741 gctcggatgg gtgggagcta ttcatccgct cggtaaaggc aaagatctct tgttggttct
     1801 cctccggaat cgtgcgttga agcaacgcgg ccagcacctt ttcatcaggg tggacgtctg
     1861 gcgggagcca gagtgccgcg cccagatagc cgtctacaca ataggcgctg ttgtgttcaa
     1921 aggctttgcc ggcgaaggcc cgcacgaaac tcggtaaatg gagcaaatat tggtgcggat
     1981 cgggatagaa ccaacgggct atgggatcgg cgctaaacgc cagcaccact gtgccgacgg
     2041 cctgatccgc gcttgatggc gttacggttt tgattgttgg tgtggtcatg gtctcgtctc
     2101 cctaaatgaa cctcgggttt tctcgcgctg gaattccaag ctaaaacgat tgtgctcact
     2161 tgtcgtccga acggcgtcgc gcttgcatcc tatgagtgac cataagaaat agccacatag
     2221 aaaagtatca gggcgaagag caaaaagccg tccatgacga catcctgttt tggtttgacc
     2281 agatcactgt ctcatgattt tacggggaac gggttgaacc gccgttcccg gttttttcct
     2341 caaggctaat gttctgattc caatgaaata catttttcgg gattgtagcg tcttttgatc
     2401 tccaccagtc gatcatggtt ggagccgtaa gtgcggcgac gcggtcaccc tcctgctcac
     2461 ccatagagca gttcgtatgc catctgattt cggatggtat tttctttcag caaattaaat
     2521 atttatcgaa agaaagaact gg
//
LOCUS       DASSWZ010000085         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_356038, whole genome shotgun sequence.
ACCESSION   DASSWZ010000085 DASSWZ010000000
VERSION     DASSWZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356038"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..913)
                     /locus_tag="VFN58_02210"
     CDS             complement(32..913)
                     /locus_tag="VFN58_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HET8916833.1"
                     /translation="MKYAFYPGCVSRGGCPELYPSAVKVADKLGIELDELKSVGCTGA
                     GVLPQNITDPINARTFAKAEQLGLPIMTICSTCTGVMSQANYRLKEDPDYREKVNKEF
                     LADEGLEYKGTTEVKHLLWVLFEDYGIEHLQSLIQRPLKGLKVSPFYGCYLRRPPEAI
                     TPTKELNRRKSYLDELISILGAEMADISGKGKCCGFPILSANEENSLAMVAKHTGESK
                     EKGADFMVTPCPLCHLNLDGNQSRAEAQTGKEIDLPILHLPQFVGLALGFDPNEMNLK
                     RHIVSTTDVESKVSMMS"
     gene            complement(910..1869)
                     /locus_tag="VFN58_02215"
     CDS             complement(910..1869)
                     /locus_tag="VFN58_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET8916834.1"
                     /translation="MEVTFKVYRKDPEKDGKPGRSNYTLELAEDATVLDALLKIRDES
                     DSTLAFRGCCGRGYCGECMMRVNGSARLACTAKVPDYTKKGPEISLDPLRNVPVIKDL
                     VFDWDAFMWNKIKAVKPWLEPDDSPVNGETLIADAEMKDLRKVMSCFYCGMCDEGCTV
                     LPVDFDFLGPAALTKAYRLAFDPRNKNSQEQVKIVEKPKGIWDCVHCYEADEHCPRNI
                     EPTKRIVALRDRAFNSGITHEAVARHHASFAASVKESGWLDEGRLAVESEGLTNVKGL
                     MKLLPTAAKALTRGKAPIPYMHSKRPDADKIKKIFEKAEREEK"
     gene            complement(1891..3513)
                     /locus_tag="VFN58_02220"
     CDS             complement(1891..3513)
                     /locus_tag="VFN58_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8916835.1"
                     /translation="MQEFDVLVIGGGASGLRAAIAAKHAGASVGLISKVHPLRTNSAI
                     AQGGMNAPLSKDDSPDSFAADTFLAGDELCERDVVKAFTQEASKEVLWLERMGVPFNR
                     DKEGRLDLRGFGSNSRNRTCYADDRTGYIVLQVLHEQFQREQIPSFAEWFVTSLVVEG
                     GTCLGAIALGLRNGKLDSFSARSIILASGGFTRFYLPSTASLRTTGDGQSLAYEAGAR
                     LMDMEMIQFHPLVFPKKHGLLITEAALNAGALIVNQKGESITDLKGMPRHKICLSIHR
                     AMQNGGGGVSLDLRPIGKEKLRGYFPQTQESLRTVAGLDITKDLVPIRPLAHRPIGGI
                     ETNAAGETSVPGLFAVGECACNGLNGAGRLAGNALTEAVVFGRRAGETAAKHATAGPK
                     KSFPAVKVADEEKQLARIFSAEPSKDSLGKIHSELGQLMNEKVGLIRSGTGLQEASGQ
                     IQNLKDRYSRLRVKNPSRIYNYELTSYLELGSMLTLAEVVALAAGARAESRGAHQRAD
                     FSERDDTNWKSHTLVSFVGGSGQLQKKPVAAG"
     gene            complement(3854..4336)
                     /locus_tag="VFN58_02225"
     CDS             complement(3854..4336)
                     /locus_tag="VFN58_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916836.1"
                     /translation="MNTAHRSLLSQGIPITNSEIASAHLARGEIAEADEEWGLALEHY
                     GIGLSLLPEDTRTVYFLFNNAAHCLNALELYSEGESYCRRAIEIDSMRPGAYKNLAVS
                     LRGQGNLRGSALCLVKAIKISPSDERAVQFLKQLLTDHPTLSLQCSQIARELELIEHR
                     "
BASE COUNT         1004 a         1268 c         1198 g         1152 t
ORIGIN      
        1 tattccacta ctccaatact ccagttttct ttcaggacat catgctgacc ttcgactcga
       61 cgtcagtcgt cgagacgata tgccgcttga ggttcatctc attcgggtca aaacccaggg
      121 ccaaacctac aaactgaggc aagtggagaa tcggaagatc aatctctttt cctgtttgtg
      181 cctctgccct cgattggttc ccgtccaagt ttaggtggca gagagggcag ggggtaacca
      241 taaaatcagc acccttctct ttggactcgc cggtgtgctt cgcgaccata gccagagagt
      301 tttcctcatt ggccgaaagg atcggaaaac cacagcactt tcccttgccg ctgatgtccg
      361 ccatttcggc gcccaaaata cttatgagct catccagata gcttttccgc cggttaagtt
      421 cttttgttgg ggttatggct tcaggggggc ggcgtaaata gcaaccataa aagggcgata
      481 ccttgagacc cttaagaggt ctttgtatca acgactggag atgttcgatg ccatagtctt
      541 caaagagaac ccatagcaga tgtttcacct cggtcgttcc cttgtattcc aggccttcgt
      601 ccgcgaggaa ttctttattc actttctccc ggtagtctgg atcttccttg agccggtagt
      661 tcgcttgcga catgactccc gtacaagtgc tgcatatggt catgatgggc agccccaact
      721 gttcggcttt ggcgaaggtt ctagcgttaa tcggatcggt gatgttttgg ggcagaacgc
      781 cggctccggt acaacctacg ctcttcagct cgtcaagctc gattcctagc ttgtcagcga
      841 ccttgaccgc cgatgggtaa agctccgggc agcctccgcg ggagacgcag cctgggtaga
      901 acgcgtattt catttttcct ccctttcagc cttctcaaaa attttcttga tcttatcggc
      961 gtcgggccgc ttcgaatgca tgtagggaat cggcgccttc cctcgggtca gagctttcgc
     1021 ggccgtggga aggagtttca tgaggccttt gacgtttgtc agtccttcgg attcaacggc
     1081 cagccttccc tcatccagcc agccgctttc cttgaccgac gcggcaaaac tcgcgtggtg
     1141 ccgggcgacg gcctcatgag tgatccccga gttgaaggcc ctgtcgcgca aggcaacaat
     1201 tcgcttggtt ggttcgatgt ttctcgggca atgttcgtcg gcctcgtaac agtgaacgca
     1261 gtcccatatg cccttgggtt tttcaacgat cttgacttgt tcctgggaat ttttgttccg
     1321 gggatcaaag gccagccgat aggctttagt cagagccgcg ggcccgagaa aatcgaaatc
     1381 cactgggagg acggtacaac cttcgtcaca catcccgcag tagaagcaac tcatcacttt
     1441 ccttagatcc ttcatctcgg cgtccgctat caaggtttcc ccgttcacag gcgagtcgtc
     1501 aggttccagc cacggtttga ccgctttaat cttattccac atgaaggcgt cccaatcgaa
     1561 gacgaggtcc ttgatgacgg ggacgttgcg cagcgggtcg agtgaaattt ccgggccctt
     1621 cttcgtatag tcaggcacct tcgcggtgca ggcgaggcgg gcactcccgt tcacgcgcat
     1681 catgcactcg ccgcagtaac cccggccaca acagccgcga aaggctagtg tgctgtcgct
     1741 ctcgtcacgg atttttagaa gagcgtccaa aaccgtggca tcctctgcca actccagggt
     1801 atagttggaa cgccctggtt tgccgtcctt ctcagggtcc ttgcgataaa ccttgaaagt
     1861 tacttccatg aaaacctcct cttccggtct ttacccggca gcaactggct tcttctgcag
     1921 ttgccctgat ccccccacaa agctcacgag ggtatggctt ttccagttgg tatcgtctcg
     1981 ttctgagaaa tccgcccgct ggtgtgcccc tcgactctca gcccgcgccc cggcggccaa
     2041 cgccacaacc tccgccaggg ttagcatcga gcccagctca agataggagg tgagctcgta
     2101 gttgtagatc ctggaggggt tcttgactcg aagccttgag tatcgatcct ttagattttg
     2161 aatctgaccc gatgcctctt gcaatcccgt gccgctcctg atgaggccaa ctttttcatt
     2221 catcaactgt cccagttcgc tgtggatctt tcccaaagaa tccttagacg gctccgctga
     2281 aaagatcctg gcgagctgct tttcttcatc cgctacctta actgctggaa agctcttctt
     2341 tggtccagcc gtggcgtgct tcgccgcagt ctctcccgcc cttcttccaa acacgaccgc
     2401 ctcggtcaaa gcatttcccg ccagccgacc agcgccgttg agaccgttac acgcgcattc
     2461 cccgaccgca aataagcccg gtactgaggt ctctccagcc gcgttagttt caattccccc
     2521 gatgggtcgg tgcgccagcg gccgaatggg cacgaggtct ttcgtgatgt ccaagccggc
     2581 aacggttcgg agcgattcct gagtctgagg aaaatagcct cgcagttttt ctttgccaat
     2641 gggtcgcagg tcaagggaga cgccgccgcc tccgttctgc atagcgcgat ggatggaaag
     2701 acagattttg tgccgtggca tcccttttag atcagttatt gattcacctt tctgattgac
     2761 aatcagcgca cctgcgttta gggctgcttc ggtgatcaat aaaccgtgct tcttgggaaa
     2821 gactaaggga tggaactgaa tcatttccat gtccatcaat ctggcgccag cttcataagc
     2881 gagagattgg ccgtctcccg tagttcgaag agaagcggtg gaagggagat agaagcgagt
     2941 gaagccgccg gaggccagga taatcgaacg cgcagaaaac gaatctaact taccattcct
     3001 taaaccgagg gctatggctc cgaggcaagt ccctccctcg actaccaatg aggtaacaaa
     3061 ccactctgcg aatgatggga tttgctcgcg ctgaaactgt tcgtgaagga cttgcaggac
     3121 gatgtacccg gttcgatcat cggcgtagca cgttcgattc cggctgtttg acccaaagcc
     3181 tctaagatcc agtcgtcctt ccttgtcccg attgaacggc acccccattc gttcaagcca
     3241 gagaacctct tttgatgcct cttgggtaaa cgccttaacc acatcacgtt cgcacaattc
     3301 gtctcctgct agaaaggtgt ccgccgcaaa actgtcaggc gagtcgtctt tgcttagcgg
     3361 tgcgttcata ccaccctgcg cgattgccga gtttgttctc agcgggtgga cttttgaaat
     3421 gagtcctact gaggctcccg catgttttgc cgcgatggct gctcgcagtc ctgaagcgcc
     3481 accaccaatg accagaacat caaattcttg catttcagta tcgcttctct tgagcgtcgc
     3541 tctcagttta actctttgac cctataacag atggctctct gaccgtcaag gaaacgaaga
     3601 ccgaaaacgc cgaagatgga ggatggctga tcgaggatcg atcatagatc catcctccgc
     3661 aaaaggatgg actgatacga gctatcggac tctcgcgaat tccattaacg ggagagttat
     3721 ctgtgagcgc gagatgcagc tttacgctat ggtttacgaa ggcggggcat tcggaaactc
     3781 gaagaggaag aacgaagaga acaaggtacg gattggaaaa cccaatatgg ccaggggggg
     3841 tgcgccggaa attttatctg tgctctatca gttccagttc gcgggcgatc tgggagcatt
     3901 ggagcgacaa ggtgggatga tccgtcaaca gctgcttgag aaactgcacc gcgcgctcat
     3961 cggaggggct aattttgatg gctttgacca agcacaaagc cgagcctctc aagttacctt
     4021 ggccgcggag cgaaacggcc aaattcttgt aagctccagg tcgcatcgaa tctatttcga
     4081 tagcgcgacg gcagtagctc tctccctcgg agtaaagctc tagcgcgttg aggcaatggg
     4141 cggcattgtt gaaaaggaaa tacaccgttc gggtgtcttc cggcaaaagc gataggccga
     4201 ttccataatg ctcaagagcc aatccccatt cctcgtcggc ctcagctatt tcaccccgtg
     4261 cgagatgggc gctggctatt tccgaattcg taatggggat accttgggat agcagggatc
     4321 tgtgggctgt gttcatgccc taaaaaagag caaagggcgc gccacgaaga aaagcctaga
     4381 aatttctaga aatatgtagt ctgtggaggt cacggcaggc aatttgacag ctcattgtgt
     4441 caaaatgtca gctcatggca cgatcgagaa tgctcgcgcc ttcgcactcc gctgattgct
     4501 attttatttt tcgatctgaa ccatcaccgt ctggtcgccg atagtttgtt ggagccgctc
     4561 gaatctaggt gatgcgttcg gagctgtagc tagggaccaa aaatatttga ttatggtttt
     4621 ct
//
LOCUS       DASSWZ010000086         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_497622, whole genome shotgun sequence.
ACCESSION   DASSWZ010000086 DASSWZ010000000
VERSION     DASSWZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497622"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFN58_02230"
     CDS             complement(<1..531)
                     /locus_tag="VFN58_02230"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916837.1"
                     /translation="MKRIWISVFCVLCLLCVPVIAGAQRLTAGYISKDLNYLPFFVGL
                     KKGMYAKEGIQVDLVSIGRADVQLQALVAGELNFANINADNVIVWNERTNGNLKVAAG
                     SSNAAPYFLIGAKNIKKVDDLKGQRLGVASLTGGATSILLAYMKSKGLVHPKDFSLAL
                     IAGGTPARLSALESNAI"
     gene            complement(802..2082)
                     /locus_tag="VFN58_02235"
     CDS             complement(802..2082)
                     /locus_tag="VFN58_02235"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8916838.1"
                     /translation="MSSLEEQLTDDVEAPAETVPRRPRLQTFSSLRHLDFRYLCAGTF
                     MMSAGQWIQQVTLGWLVYELTGNSVLLGALNGLRALPFLVTGPMAGVAADRMDRQKLM
                     LRTQYLLIVTAVVMGGLVVSGWLQVWHIFVFTLITGIGWTFSEPVRQSLIPSVVPKAE
                     LANAIALNSGGFNLMKVIGPALGGVMIAVFGAGGNFFVQGVAYVGVLVMIYLMHVPPT
                     PEKARHSSAMANLKQGFAYVWSTPAVLALMTLAYVPRVFAVPYQTLMPVFQKDVLKVG
                     PEGLGLLMAAPGVGAVLSVLVLASLTNRVKRQGVLLVGSIIVLGAFLILFSQITSFPL
                     ALIVLILVGGFQMLFLASTNTMLMLIVPDELRGRVMSLYMLDRGLMPAGALFAGVVAH
                     FIGAPSTVATMGAIVIVLTLVVAWRIPAIRALET"
     gene            complement(2136..2861)
                     /locus_tag="VFN58_02240"
     CDS             complement(2136..2861)
                     /locus_tag="VFN58_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8916839.1"
                     /translation="MAEPILTEKQADVTTITLNRPEAGNRQTDATWAEVTKTIDAAAQ
                     DSRVILFKGAGDDFCLGREAMGQPGPVLEAYGVRERSDTIFNLYGAFRNAKVPIIAVV
                     QGRAVGLGCALAALCDITIASDRARFQLPEMSHRIMPTIAMSALVDRVPRKAATYLVY
                     STQEIDAQKALTFGLVSNVVPPAELDAAVANLVEHFKKTPLAALLAVKEYARSAFSMS
                     TQAANDLARNLHATVNSFSGMRA"
     gene            complement(3016..3279)
                     /locus_tag="VFN58_02245"
     CDS             complement(3016..3279)
                     /locus_tag="VFN58_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916840.1"
                     /translation="MAEETPEQKLARLEAENRALREQVEQRRPGQLRLKVSEKGGVSV
                     YGLGRFPVTLYKEQWIRLLEHADAIKSFLKENDQSLKAKQQEK"
     gene            complement(3349..>4388)
                     /locus_tag="VFN58_02250"
     CDS             complement(3349..>4388)
                     /locus_tag="VFN58_02250"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916841.1"
                     /translation="ALIAAMLPLSSISTFAQSSPPAKLQKVTALYGARSGASWPMWMA
                     KDGGYYRKHGLDVELVFGVHPAPIAAVISGQAAMTPAGADPALLSASKDPSLVLLGSY
                     LNKGSFALVAAKNINQINQLAGKRIGVGRVGDPPYHFTLSLLRKHGINPKDIQWLSVG
                     VDAAARAASLHTGQIDATLVTAPSYFRLEAAGFPVLAVLSDYDDIYVSTYYLFRRDAS
                     PTNRMVAEGFIKAHAEAIKRFYDDKAFATQVMMKYGGAKDMEDAGRVYHLFAKSRMFE
                     SVPYVLKGSVEGVIERQRQEQPYLKDFDFSKSVDNSLVDQLVKEGFFERVFGPAIREE
                     QLKKQALSFGR"
BASE COUNT         1015 a         1264 c         1183 g          926 t
ORIGIN      
        1 gatagcgttg ctctccagcg cgctcaaccg cgccggtgtg ccgccggcaa tgagcgccag
       61 ggaaaagtcc ttgggatgga cgagtccttt actcttcatg taagccagca aaattgaagt
      121 cgctcctccc gtcaggctgg caaccccaag cctttgccct ttgagatcat cgaccttttt
      181 gatgtttttc gcgccgatga ggaaataggg cgcggcattg ctcgacccgg cagcgacttt
      241 gaggttgccg ttggttcttt cattccagac gataacgttg tcggcgttga tgttggcgaa
      301 gttaagctcg ccggcaacca atgcttgcag ttgaacatcg gctcggccga tgctgacgag
      361 atcgacctga atgccttcct tggcatacat gccttttttc agaccgacaa aaaacggcag
      421 atagttaaga tctttcgata tgtagcctgc ggtcagtctt tgcgcgccgg cgataacggg
      481 tacacaaagc aaacacaaca cgcagaaaac ggaaatccaa atccttttca tggtttcact
      541 tgctcctttc gcgccggacg atcacttagc ccggaaaaat tcatgctctg aagaaaaatc
      601 tcggcagagt atcgctcgcg gagtgagcct ggaagggcgc gggcacaaga acctaaagct
      661 gcgttgactt ctatggtgct tggccatcag tttgcgtctc tcccttactg cacgttccac
      721 caactagatt tgcaggccgt ctcaagatag agacccattg cccgcaatcg gagaggctcg
      781 gcgcttctgt gaacaaccag gttaggtttc taaagcacgg atcgcaggaa tccgccatgc
      841 gacaacgagc gtgagaacaa tgacgatggc tcccatcgtc gccacggtgg acggcgcgcc
      901 gatgaagtgc gccaccacgc cggcaaacag tgcgccggcc ggcatgaggc cacggtccag
      961 catgtaaagg ctcatcacgc ggccgcgcag ctcgtcgggg acgataagca tgagcatcgt
     1021 attggtgctg gccaggaaca gcatctgaaa accacccacc aaaatcaaaa ctatcagcgc
     1081 cagcggaaag gaagtaatct gagaaaagag aatgagaaaa gcaccgagga cgataatgct
     1141 gcctaccaac aatacgccct gacgttttac tctgttagtg agggaggcca aaaccagcac
     1201 ggacagtacg gctccgactc cgggtgcggc catcagcaag ccgagacctt ctggacccac
     1261 cttaagtacg tccttttgaa acaccggcat caaggtctgg tagggcaccg caaagacacg
     1321 cgggacatag gccaaggtca tgagcgccag aaccgcgggg gtggaccaaa catacgcgaa
     1381 gccctgcttg aggttagcca ttgccgaaga gtggcgcgcc ttctccggtg tcggcggcac
     1441 gtgcatcaaa tagatcatga ctaatacacc cacgtatgcg acgccctgca cgaaaaagtt
     1501 tcccccggct ccaaacacgg cgatcatcac accacccagt gcgggaccga tgaccttcat
     1561 gagattaaat ccgcccgagt tgagcgctat cgcgttggca agttccgctt tgggaacaac
     1621 gctcggaatc aggctttgcc ttaccggttc gctaaacgtc cagccaattc ccgtgatcaa
     1681 cgtaaacaca aaaatgtgcc acacttgtaa ccatccggat acgacaagcc cgcccatgac
     1741 gaccgcggtc acgatcagca agtattgagt gcgcagcatc aatttttgcc ggtccatgcg
     1801 gtcggcggcg acacccgcca tggggccggt gactaaaaac ggcagcgcgc gcaagccatt
     1861 gagcgctccc agcaacacgg aattgccggt cagctcatac acgagccatc ccaaagtcac
     1921 ttgttggatc cactgtccgg cactcatcat gaacgtgccg gcgcaaagat agcgaaaatc
     1981 cagatggcgg agagaggaaa aggtttgaag ccgaggacgc cggggaaccg tttcagccgg
     2041 cgcttcaaca tcgtcagtca gttgctcttc tagagaactc atttcgcaat catagcttga
     2101 ttgcccttag cttgccacgt ttccaagaat gctttttagg cacgcatccc ggaaaaactg
     2161 ttcaccgtgg cgtgcaggtt gcgcgcaaga tcgttggctg cctgggtgct catgctgaaa
     2221 gcggagcgcg cgtattcttt gactgccagc aaagcggcga gcggcgtctt tttaaaatgc
     2281 tcgacgagat tcgcgacagc ggcatcgagc tcagcaggtg gaaccacgtt gctcaccagc
     2341 ccgaacgtga gcgccttctg cgcgtcaatc tcctgggttg agtagactag ataagtggcg
     2401 gccttgcgcg gtacgcgatc caccagcgcg gacatggcga tggtcggcat gatgcggtgc
     2461 gacatttcgg gcagttgaaa gcgggctcta tcgcttgcga tggtgatgtc gcagagcgcc
     2521 gccagggcac agcccaggcc gacggcgcgg ccctgaacca cagcgatgat gggaaccttc
     2581 gcgttgcgaa acgcgccgta gagattgaaa atcgtatcgc tccgttcgcg aacgccatag
     2641 gcttcgagaa ccgggccggg ctggcccatc gcctcgcgac cgaggcagaa atcgtcccct
     2701 gcccctttga ataagatcac acgcgagtcc tgcgccgcgg catcgatcgt cttcgtgacc
     2761 tcagcccagg tggcgtcggt ttgccggttg ccagcctccg gacggtttaa agtgatcgtc
     2821 gttacgtctg cctgtttttc cgtgagtatg ggttccgcca tgaaaatgac ctccagtcga
     2881 attttttcaa ctctaaaaag agcctgcccg atttgtcaat gggattctag attcccgcgt
     2941 tcgcgagaat gacgaccgag agcgactgaa ttcctcgtcg aatttctgag atctgacaat
     3001 aaccccgagg cgttgctatt tttcctgttg tttggccttc aacgattgat cgttctcttt
     3061 gaggaatgac ttgatcgcat ccgcatgttc gagcagcctg atccactgtt ccttgtaaag
     3121 agtcacgggg aatcggccaa gaccgtagac cgaaacgcct cccttttcac ttaccttcag
     3181 acgcaattga cccggtctgc gttgctcgac ctgttcgcgt agtgctctat tctccgcttc
     3241 gaggcgagcc agtttttgct ctggggtttc ttccgccata acacctccgt aaaatcattt
     3301 agacctatct gtcaaatctc cagtgcacaa aaagacatga caagaatcct atctgccaaa
     3361 ggaaagcgcc tgcttcttta gctgttcctc tctaatcgcc ggcccgaaaa cccgctcgaa
     3421 aaaaccttcc ttgacgagct ggtcgaccag gctgttgtcc acggacttgc tgaaatcaaa
     3481 atccttcaag tagggttgtt cctggcgttg tcgctcaatg acaccctcta ccgagccttt
     3541 gagaacgtag ggcaccgact cgaacatccg gctcttggca aacaggtgat aaacccgccc
     3601 ggcatcttcc atgtctttgg cgccgccgta tttcatcatc acttgcgtag cgaaggcctt
     3661 atcgtcgtag aaacgcttga tcgcctcggc gtgggccttg atgaaacctt ctgcaaccat
     3721 tcggttggtc ggcgaagcat cccggcgaaa aaggtaatag gtggaaacat agatatcgtc
     3781 gtaatcggag agcaccgcga gcacgggaaa tcccgcggcc tcgagcctga aatacgaagg
     3841 cgccgtgacc aaggtggcgt caatctgacc cgtatgaagg ctcgcagccc gcgcggcggc
     3901 atccactccc accgacaacc actgaatgtc cttgggattg atcccgtgct tccggagcag
     3961 gctcaaggta aagtgatagg gcgggtcgcc cactcgaccc acaccgatgc gcttaccggc
     4021 aagctggttt atctgattga tgttcttggc ggccaccagg gcaaagctcc ctttgtttaa
     4081 ataacttccc aggagcacca atgaaggatc tttggatgcc gataacagtg cagggtccgc
     4141 tcctgccggg gtcatggctg cctgcccgct gatgaccgcc gcgatgggcg ccggatggac
     4201 gccaaacacc agctcaacat cgaggccgtg cttacggtaa tagccgccgt ccttagccat
     4261 ccacatcggc caggaggcgc cgctccgcgc gccatagaga gcggtgactt tctgcaactt
     4321 ggccggcggc gagctttgag caaatgtcga aatcgaactc agtggcagca tcgccgctat
     4381 gagagcga
//
LOCUS       DASSWZ010000087         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_412816, whole genome shotgun sequence.
ACCESSION   DASSWZ010000087 DASSWZ010000000
VERSION     DASSWZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412816"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1431)
                     /locus_tag="VFN58_02255"
     CDS             complement(178..1431)
                     /locus_tag="VFN58_02255"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET8916842.1"
                     /translation="MIERAVILSTGDELTTGRVVDTNSSAIADRLFAVGVEVAAVLTV
                     SDDREKLHWALRQGRELGDVIIGTGGLGPTADDLTTEVVAEFLGSQLVQDEHVAGSLK
                     RRFEARGIAWTPNNLKQALFPEGSVIVPNPIGTAPGFQVPIGPGKTLIWLSGVPQEMT
                     AMLNDTVIPWIVRQRGDAEQVTASTFKIYGLTESKLDDLMKPVKLGSDARLSFRAHYP
                     DLSLRLMVKGGKESAETLTRLKDQIRGALGSFIYFEGDATLEEVVGQLLLAKQQTLAL
                     AESCTGGYISHRITRVAGSSAYYHGGLVTYSNDAKIRFLNVKPATLESYGAVSRETAL
                     EMSRGIRERSGAGIGLSVTGIAGPLGGSPDKPVGTVWISIAQEAGHEARLFRFHGDRE
                     RIIIGTSQAALHWLRTVLLSETAEP"
     gene            complement(1557..2807)
                     /locus_tag="VFN58_02260"
     CDS             complement(1557..2807)
                     /locus_tag="VFN58_02260"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET8916843.1"
                     /translation="MKPTAVQGSNPKTLRKEAPKSKTTAPAPPIRRLTKAQSEVPADA
                     MERREHVIQQTRVGDTLLELLSRFGLANAERQLWTRSIQRNIGTRGLLPAGKEVHFYF
                     TKTIPGTRQSPQLKALEVDFSDTSTLTWEKGIRGVLFQRREKPYDIELKTVGASVENS
                     LFADGQKAGIHPTLLSQLADIFTWDIDLEREVRKGDSIKILYEQRSRKGQETKASLRI
                     LAAELISADQKLTAIYFEKQKGQGNYYNLEGRSLARSFLRFPLEFTNITSHFAPARFH
                     PILKVNIPHTGVDFAAKRGTPVRAVGSGLITLADWNGVYGKTVEIQHDSTYTTRYAHL
                     ERFAEGVHNGAAITKGQIIGYVGSTGRATGPHLHFELYKDQQYIDPLSVDFPAEDLIE
                     PTLQALFDNQKHILLVELSSAPQS"
     gene            complement(3049..>3874)
                     /locus_tag="VFN58_02265"
     CDS             complement(3049..>3874)
                     /locus_tag="VFN58_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET8916844.1"
                     /translation="PPEESKGERELTPIQERGSELLAGFSVRSYPRLFGYMETELKRG
                     AHLDLMLPREDRKIPLLASWRYEKGRVAAFTADLEGRWTRNWIQWSALQTFWDRLLGW
                     LRPSDERDTIPVHEARVGLSANKPFLDLFLYEDTGADGQFRFSVNGKGSKAEGVLQKL
                     APGHYQAALPLAAPGDYRVELTEERRGRRIAYPPIGYSFPYELNSELPRPDFNLPLLN
                     TLAQASGGEINPKSIESSEKQDFTNTYRPLRQALIMCAAALFLFEIVARKLFLSEA"
BASE COUNT          863 a         1020 c         1059 g          932 t
ORIGIN      
        1 aataggttcg attctacttt cctcaatgtc acccaggaac ttgagcgtca agtggaagtt
       61 cgtcggcggg acccagcgaa tacctggaat acggggtttg aggtcggtga cggccgcaga
      121 gatctgtccg acagtttgcg aatcgatctc gacggcgata aaagcgcgaa tcacggacta
      181 cggctcggcg gtttctgaca gcagcactgt gcgcaaccag tggagggcgg cttgcgatgt
      241 gccgatgata attcgctcac ggtcgccgtg aaaccggaag agcctagctt catggccagc
      301 ctcttgggcg atacttatcc agacagtccc tacgggcttg tccgggctgc cacccagggg
      361 accggcgatg ccggtaacac tgaggccgat gccggctccg cttcgctctc tgatccctcg
      421 ggacatttct aaagcagtct cccggctgac cgcgccatag ctttccaggg tggccggctt
      481 gacgttgagg aagcgtattt tggcatcgtt ggaataagtt accaagccgc catggtaata
      541 agctgagctg ccggctaccc gtgtgatacg gtgactaatg tagccgccgg tacaggactc
      601 agcgagggcc agggtttgct gctttgccaa aagtagctgt ccgacaactt cctctaaagt
      661 cgcgtcgcct tcaaaataaa tgaaggaccc gagggctccg cgaatttgat ctttgaggcg
      721 ggtcaaagtt tcggcgctct ccttgcctcc cttaaccatc agacgcaaag agaggtcagg
      781 atagtgggcg cggaatgaga gtctggcatc cgatccaagt ttcacgggct tcatcaggtc
      841 gtccagcttt gactcagtta ggccatagat tttgaatgta gaggcggtga cctgttctgc
      901 gtcgcctctt tgtcgcacga tccagggtat taccgtgtcg ttcaacatcg ccgtcatctc
      961 ttgcggtact ccggacaacc aaatgagggt tttgccagga ccaatgggga cctggaaccc
     1021 gggggccgta ccgatgggat tgggaactat caccgatccc tccgggaaca gggcttgttt
     1081 caggttattc ggtgtccaag cgatgccgcg ggcctcaaag cgccgtttca aggaccccgc
     1141 gacgtgctcg tcttgtacca gttgggaccc aagaaattcg gccacgactt cggtggtcaa
     1201 atcgtccgcc gttggcccaa gtcctccggt gccgatgatc acatccccga gctcgcgtcc
     1261 ctggcgcagg gcccagtgga gtttttctcg atcgtcagag actgtgagca ccgccgctac
     1321 ttcaaccccg actgcaaaaa ggcgatccgc gatagccgag gagttggtat cgacgactct
     1381 gccggtggtg agctcatcgc cggtgctcag aatgaccgca cgttcaatca tacgcttcct
     1441 gacttgccca tcggaaacat cttttcgata tcaaaaaaac aaaagaatac aaagctgtgc
     1501 cgaatagggc gggtttggaa aaagcgcccc aaacacctgg aggagtagac cgtgatctat
     1561 gactggggtg cggagctaag ctcgacaagg agaatatgtt tttggttatc gaaaagcgcc
     1621 tgcagggtag gctcgatcag atcctcggct gggaaatcaa cgctcaaggg atcgatgtat
     1681 tgttgatcct tatagagctc gaaatgcaga tgcgggccgg ttgccctgcc ggtggatccg
     1741 acataaccga tgatctgtcc cttggtgata gccgcgccgt tatggacccc ttcggcaaac
     1801 ctctccaggt gggcgtaacg agttgtgtac gtggaatcgt gttggatttc caccgtcttt
     1861 ccgtaaacac cgttccagtc agcgagtgta attaaaccac taccaacggc ccggaccggc
     1921 gtgccccttt tcgcggcgaa gtctaccccc gtatggggta tattgacctt aaggatggga
     1981 tgaaagcggg cgggcgcgaa gtgcgaagtg atgttggtga actccagagg gaatcggagg
     2041 aaggagcgag ccaggcttct tccctctaaa ttatagtagt ttccctgtcc tttttgcttc
     2101 tcaaagtaga tggcggtcag tttctggtcg gcgctaatca gttcggcggc tagaatacgg
     2161 agcgaggcct ttgtttcctg tccttttcga cttctctgct catacaatat tttgatgctg
     2221 tccccttttc gaacttctct ttcaaggtcg atatcccaag taaagatatc ggcgagttga
     2281 gagagtaaag tcggatggat acctgctttt tgcccatctg caaagagcga gttttccacc
     2341 gatgcgccga cagtcttcag ctctatgtca tagggcttct cgcgtctctg aaagagaacc
     2401 cctctaattc ctttttccca ggtgagggtt gaggtatcgg aaaaatcgac ttccagtgct
     2461 ttcagctgtg gactctgacg agtgccaggt atggttttag taaaatagaa atggacctcc
     2521 ttaccggccg gcagcagccc ccgagttcca atattgcgct gaattgaccg cgtccaaagt
     2581 tgtcgctcgg cattcgccag gccaaaacga ctcaataact ccagcaatgt atcgcccact
     2641 cttgtctgct ggatgacatg ctcgcgcctt tccatggcgt ccgcggggac ttcgctctgg
     2701 gccttagtga gtcttcggat cggcggtgct ggggcggttg ttttgctctt gggagcttct
     2761 ttgcggaggg tttttggatt agatccttgg acagccgtgg gtttcattgc gaaaacagaa
     2821 ccgataaccc aaagccaaac taaggttcta agaacagagg ggaatgctga aagctggtgt
     2881 cggccggaaa acgttccccc ggtgcctgta tgtttgattt ccaggcccat aacaagaagt
     2941 tctcattgaa taaaccgttt tgattaccgt gtcaaacaaa aaattcgccg gctagacgtt
     3001 cgatttcgag cacttgggaa aggatcggta gctggatggt tggttaattc aagcctctga
     3061 aagaaacagc ttcctggcaa caatttcaaa aagaaaaagc gcagctgcac acataattaa
     3121 agcctgacgc agaggccgat aggtgttcgt aaagtcttgt ttttccgagg attctatgga
     3181 cttcggatta atttctccgc cgcttgcttg agccagtgta ttcaacaaag ggaggttaaa
     3241 gtcgggtcgc gggagttcgc tgttaagctc gtaaggaaaa ctgtagccaa tgggcggata
     3301 ggctatcctt cgcccccgcc gctcttctgt cagctcaaca cgataatctc ccggagccgc
     3361 taaagggagg gcggcctgat aatggccagg ggccagcttt tgtagtactc cctctgcctt
     3421 gctccccttt ccattgactg aaaaccgaaa ctggccgtcc gcaccggtat cctcgtagag
     3481 aaacaaatcc agaaaaggtt tgttggcgga aagacccacc cgcgcctcgt gaacgggtat
     3541 ggtgtcgcgc tcgtcgctag gacggagcca accgaggagc ctgtcccaaa acgtttgcaa
     3601 agcgctccat tggatccagt tcctggtcca acggccttcc aagtccgccg taaaggctgc
     3661 gactcttccc ttctcatatc gccaggacgc caataagggt attttccggt cttctctcgg
     3721 aagcatgaga tccagatgag cccctcgctt tagctcagtc tccatgtaac caaagagcct
     3781 tggataggat cttactgaaa agcctgcaag taactctgat cctctctctt gaatcggggt
     3841 cagctcccgt tcacccttgg attcttccgg aggg
//
LOCUS       DASSWZ010000088         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_58949, whole genome shotgun sequence.
ACCESSION   DASSWZ010000088 DASSWZ010000000
VERSION     DASSWZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58949"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFN58_02270"
     CDS             complement(<1..938)
                     /locus_tag="VFN58_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916845.1"
                     /translation="MGKNEIRPNRVAGPVMVALTICILFLGSSWVEAAQSRNFNEKAA
                     ADFYRGKTVTIIVGFAPGGGYDISARLVARHLGKHIPGTPNLIVENRPGAGSLVAANL
                     VYKGLRPDGTYVATFNSQMVLQQLLGQQGMEFDSRAFNWLGSVSSSQSACGVHRDTGV
                     THVKQLMGPSGKVVNMGGEAPGSGITDTAAVIRAAVGAKFKIIYGYAGSRPVANAVLN
                     RELDGMCISWESFTSDLKMFFEPQKLLNMLVIFGTEVPKHPWLENAEAAEAIAPTNDA
                     RTLLKLVYAPGKMSFPYAVAPGVPADRLAALRVAFD"
     gene            complement(986..>2048)
                     /locus_tag="VFN58_02275"
     CDS             complement(986..>2048)
                     /locus_tag="VFN58_02275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916846.1"
                     /translation="DEAGLPVRSASATTLDKSLVPHRVCDRERNSEVVGIVDIHAHWY
                     PDGCIAEAIGGQSDFRLDSVPEGGQRLLCRGSYVMSLPRGHNDMEGRLSVMDQAGVAV
                     QVLSVGALDIGLARTRASVVARRINDALSEICVKANGRFRFVASLALDDPAELIKEMD
                     RMAAMGAVGVGITTTVGGRSLDADEFRGFWQEAGRRELVVLVHPTFPLTGPAGDRGEF
                     LTTAYLGETAMAATKVVLAGILDESPRVRIVWSHLGGGLAMLIDRLDRVYKRYPTCPK
                     PPSFYLKRCFYDTVCTHGPALDCARATFGADRLVFGTDEPHVPNGTGDVLAALRNRSW
                     PAADLEAVLTQNVTRLGVL"
BASE COUNT          458 a          663 c          506 g          421 t
ORIGIN      
        1 ttatcaaagg cgacgcgtag agccgccaat cgatctgctg gcacacccgg cgccaccgca
       61 tagggaaaag acatcttccc tggagcgtag acaagcttca aaagcgttcg cgcatcatta
      121 gtcggagcaa tggcttcggc cgcttcggcg ttttccagcc atggatgctt gggcacctcg
      181 gtcccaaaga tgaccagcat attgaggagc ttttggggct caaagaacat cttcaggtcc
      241 gaagtaaaag attcccaact gatgcacatc ccgtccagct cacggttcag aaccgcattg
      301 gcgaccggcc gtgaccccgc atagccgtag atgattttaa atttggcccc gacagcggca
      361 cggatcaccg ccgccgtatc ggttattccc gaaccgggcg cctctccccc catgttcact
      421 acctttccac tcggccccat caactgctta acgtgagtca ctcccgtgtc cctgtgaacc
      481 ccgcacgcgc tttggctcga cgacacgctc ccgagccagt taaacgcccg gctgtcgaac
      541 tccatccctt gttggcctaa aagctgttgc aacaccatct gggaattgaa ggtcgcgaca
      601 taggttccgt ccgggcgtaa gcccttatag acgaggtttg cagccacaag gctgccggca
      661 ccgggacgat tctccacgat caaattggga gtgcccggta tgtgtttacc tagatgtctg
      721 gccaccaatc gcgcggaaat atcgtaaccg ccacccggcg cgaatccaac gataatagtt
      781 acagtcttgc cacggtaaaa atcagctgcg gctttttcat tgaagtttcg ggattgtgca
      841 gcctctaccc aacttgaacc caggaagagg atgcagattg tcagagctac catcaccgga
      901 cctgcgaccc tattgggtcg aatttcattt tttcccatct aacctcctcg gtaatctccc
      961 tgcagcaacg cttcctttta agctcctaaa ggacgcccaa ccgcgtcaca ttttgtgtca
     1021 agacagcctc caggtccgca gccggccagg accggttacg gagagccgca agcacatcgc
     1081 ccgtcccgtt gggtacgtga ggctcatcgg tcccgaacac gagccgatcg gcaccaaagg
     1141 tcgcgcgggc gcaatccagc gccgggccat gggtacagac ggtatcgtaa aaacatcgct
     1201 tgagataaaa gctcggcggt ttcggacaag ttggataacg cttgtaaacc cgatccaatc
     1261 gatcgatcag catggccagg ccgccaccca agtgagacca gactatccgc accctgggag
     1321 actcatcgag gatgccggct aagacgacct tggtcgccgc cattgccgtt tcccccagat
     1381 aggccgtcgt taaaaactca ccccggtcac ccgccggtcc tgtcagaggg aaagtcgggt
     1441 gaactaagac gacaagctcg cgtcgcccgg cttcttgcca aaacccgcga aactcatccg
     1501 catccagtga tcttcccccg accgtggtcg tgattcctac gcccaccgca cccatcgcgg
     1561 ccatccgatc catttctttg atcaactccg cgggatcgtc cagcgccaaa gacgccacga
     1621 agcgaaacct cccgttggct ttcacacaga tctcggagag agcgtcgttg attcgcctcg
     1681 cgaccacgga ggcgcgcgtc cttgccaaac cgatatccag agcgccaacc gacagaacct
     1741 gaactgcaac tcctgcctga tccatgaccg agagccgccc ttccatgtca ttatgcccgc
     1801 gcggtagcga cattacgtag ctcccgcgac acagcagccg ctgtccaccc tccggcacgc
     1861 tatctagacg aaagtcgctt tgaccgccga tggcttcggc tatgcatcca tccgggtacc
     1921 aatgcgcgtg tatgtcgaca attcccacga cttcgctatt tctttcccta tcgcagactc
     1981 tgtgaggaac cagactcttg tctaacgtcg ttgccgaagc agatctaaca ggtagtccgg
     2041 cttcatcg
//
LOCUS       DASSWZ010000089         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_101419, whole genome shotgun sequence.
ACCESSION   DASSWZ010000089 DASSWZ010000000
VERSION     DASSWZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101419"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..2010
                     /locus_tag="VFN58_02280"
     CDS             79..2010
                     /locus_tag="VFN58_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HET8916847.1"
                     /translation="MARKRYLKKTPLEEARKLFLASIDASKLASETVPVEDALNRVTA
                     EPVFAKISSPHYHAAAMDGICVRAEDTFGATEFVGKKLTRASKDVSGPDAFAYVDTGN
                     SLPAWANAVIMIEKVRQLADETVEIFESVAPWNHVRLVGEDVVATELLLPRSHRLRPY
                     DLGALLASGHTSVRVKVHPKVGIIPTGDELIQPGEEAKPGAVIEFNSTVLAAFVREWG
                     GVPVKYPKVKDDAELLKQALGRAADECDVATIIAGSSAGEHDLTADVVAAAGELWAHG
                     IDVMPGKPAVLGAADGKPVIGFPGYPVSAIVIAREILKPGLEKFLGCAAPSYPRVRAV
                     VPKKLASHLGLEEFIRVTLGRVGEKLVAVPLARGAGVITTMVRADGLLRIPSFIEGLN
                     AGEEISVELLRPEEEIENTILCTGSHDLAIGVLEDQLKLQHAGLKIAATNVGSLGGLL
                     ALQRGETHIAGTHLLDPETGVYNIPDIKKTIPKTPVVLVHLAQREQGILVLRGNPKSI
                     RGLNDLARKDCRFVNRQPGSGTRVLLDYELKRLRIDPASIHGYEREEFTHMAVGVAVA
                     SGLADAGLGVRAAAQALALDFIPLANEQYDLLCLRPFYESARGTQLMAIIRSQSLKTA
                     VASLGGYDPVKAGEVLYQQ"
     gene            2289..2504
                     /locus_tag="VFN58_02285"
     CDS             2289..2504
                     /locus_tag="VFN58_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8916848.1"
                     /translation="MDLENIRLLTLSEAAILLQVSTRTLQRMIRNGQLPAFKVGGQWR
                     LRETQLRQWVENRETAVVKLEKESREI"
     gene            2501..2797
                     /locus_tag="VFN58_02290"
     CDS             2501..2797
                     /locus_tag="VFN58_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916849.1"
                     /translation="MKIGVFSGLRSRPVCLIAGRLTEKENFPGEDEWARAATRFETGV
                     GGPEIKDPKNKKVEKDLHRHLAWMQMLCEVDRKFLASLDLRLMTDALFTVALPG"
BASE COUNT          654 a          775 c          882 g          693 t
ORIGIN      
        1 gagcagggag aggaagtcga ggtgataatg ttttagtctc gagacctgag actcggaacc
       61 cgagacaata aatttcccat ggcgcgcaag cgatatctaa agaaaactcc tttggaagag
      121 gcccgaaagc tttttttggc aagtatcgat gcgtcaaaat tggccagcga gacggtaccg
      181 gttgaggacg cgcttaatcg cgttaccgca gagcccgtgt tcgcgaagat ctcatcgccc
      241 cattaccacg ccgccgccat ggacggcatc tgtgtccgcg ccgaggatac gtttggcgcc
      301 actgagtttg tcggaaagaa acttactcgg gcctcgaagg atgtctcggg accggatgcc
      361 ttcgcttatg tggacacggg caactctctc cctgcttggg ccaatgcagt cataatgatt
      421 gaaaaagtgc ggcaactcgc tgatgaaacg gtggagattt tcgagtcggt agcaccctgg
      481 aatcacgtcc gactggtggg cgaggatgtc gtcgccacgg aattgctttt gccgcgctcc
      541 caccggttgc ggccctacga tctgggcgca ttgctcgcgt cggggcacac ctcggtaagg
      601 gtaaaggtcc atcccaaggt ggggatcatt ccgacggggg atgagttgat ccagccgggt
      661 gaagaagcaa aacccggggc ggtcatcgag ttcaactcca ccgtgctggc ggccttcgtg
      721 cgcgaatggg ggggagtgcc ggttaagtat cccaaagtta aagacgatgc tgagttgctc
      781 aagcaggcgc taggtcgggc ggctgacgag tgcgatgtgg ctacgatcat cgccggatct
      841 tccgccggcg agcatgactt gaccgcggat gtggtcgccg ctgcgggtga attgtgggcc
      901 cacggcatcg acgtgatgcc tggaaaacct gcggtcttag gcgcggcgga tggcaagccg
      961 gtgatcggct ttcccggata tccggtgtcg gcgatcgtca tcgcgcggga aatcttaaaa
     1021 ccagggttag agaagtttct tggttgcgcg gcgccgtcct atcctcgagt tcgtgccgtc
     1081 gtgccgaaaa agctcgcgtc tcatctcggc ctcgaagaat ttatccgcgt aacattgggg
     1141 cgcgttggag aaaaattggt cgccgtgccg ctggcgcgcg gcgccggggt gattacgaca
     1201 atggtgcgcg ccgatggatt attgcgcatc ccaagtttta tcgaaggctt gaatgcaggc
     1261 gaggaaatat cggtggaact gctgcggccc gaagaggaaa tcgaaaatac catcctctgt
     1321 acggggagtc acgacctcgc catcggcgtt ctcgaagacc aacttaagtt gcaacacgcg
     1381 ggactaaaga tcgcggcaac gaatgtcggc agcttaggag gcttgctggc tttgcagcgg
     1441 ggtgagaccc atatcgccgg cactcatctg cttgaccccg agacgggcgt ctacaacatc
     1501 cctgacataa aaaaaaccat tcctaaaact ccggtcgtct tggttcatct ggcgcaacga
     1561 gagcaaggta ttctagtgct gcgcggcaat ccgaaatcga tccgtggcct aaacgatctt
     1621 gcgcgaaagg attgccggtt tgtcaatcgg caaccgggtt ccgggacccg tgttttgctc
     1681 gattatgagc tcaaaagatt gagaattgac ccggcttcca ttcatggcta cgagcgggaa
     1741 gagtttaccc atatggcggt cggggtggca gtcgccagcg gtctggccga tgccggactc
     1801 ggtgtgcggg cggcagccca ggccctggcg cttgatttca ttccgctggc caacgagcag
     1861 tacgacttgt tgtgtctgcg cccgttttat gaatccgcaa gaggaacaca gctaatggca
     1921 atcattcgct cccaatcctt aaagacagcg gtcgcctccc tcggcggtta tgatccggtc
     1981 aaggccggag aagttcttta ccagcagtaa ctctaccgtc gttattttcc ccattccaag
     2041 tcatcgcggc cgcaggttat cccggttaac gacaacgccg ttcagctccc cgcgacatcg
     2101 aactatttgt gcagtttttt ccaagctgtt ccgccactgc actagctagt cgtggtttgt
     2161 cccgggttgt ccatttttgt acttgaagag atccaacttc tgttgcgctt ccctttcgaa
     2221 aacagtcagt tagatgcaag tttttgctgg cattggcatt gcgtttcgcc tccctgagga
     2281 aattttcgat ggatctagaa aacattcgtt tactcactct ttctgaggct gcgattcttt
     2341 tgcaggtttc aacgcggacg ctgcagcgga tgattcgcaa tgggcaactg ccggctttca
     2401 aggttggagg gcagtggcgt ctgcgtgaaa cacagttgag gcagtgggtg gaaaataggg
     2461 agactgcggt tgtcaaactt gaaaaagaga gtcgtgaaat atgaagattg gcgtgttctc
     2521 gggtcttcgc tcgcgaccgg tttgcctcat tgccgggcgt ctaactgaaa aagagaattt
     2581 tcccggtgag gatgaatggg ctcgggcggc tactcgtttc gagacggggg tcggtggccc
     2641 agaaatcaaa gatcccaaaa acaagaaagt agaaaaagat ttgcatcgcc atcttgcgtg
     2701 gatgcagatg ctttgcgaag tggaccgaaa attcctcgcc agcctcgatc tccggctgat
     2761 gacggacgcg ttgtttacgg ttgctctgcc gggctagcag gctggaagca gtctttttag
     2821 caaccagcaa gtcctagaat gagctttcaa tagcgggcag cggagtccaa gggtgaaaaa
     2881 cctgcacaaa gaaaaatccc ctctgagttc atctcctagg tacggacttc gtgtgcgcct
     2941 cattctgctg gtatttattg ccatcgtccc ggcgctcgtc ctcatcctcc ataacgccaa
     3001 gcgt
//
LOCUS       DASSWZ010000090         6062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_384523, whole genome shotgun sequence.
ACCESSION   DASSWZ010000090 DASSWZ010000000
VERSION     DASSWZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384523"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFN58_02295"
     CDS             <1..495
                     /locus_tag="VFN58_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8916850.1"
                     /translation="DHETTANIGVIEGGMAVNIVSNSVVLRGEARSHSEEKLQRQTQH
                     MLQCLHEAASRHVLELDGKRFQAKVEAKIDRDYDHMHVPDDSAIVRLVQRAAKNLDLE
                     LKTLATGGGCDANVLNKKGLEVANLSTGMREIHTVNEWLDLKDLYLSAEVVLETVRLN
                     GASH"
     gene            557..2002
                     /locus_tag="VFN58_02300"
     CDS             557..2002
                     /locus_tag="VFN58_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8916851.1"
                     /translation="MDAEEIGLLLKQERGQFLEFVSAYEYRRGGTQKKRDEDLAREIV
                     RVLSGMANADGGTLLVGVEPDRSVTGLPHNPNEVQSLIQAPQTLLTPPLSLASAKIRL
                     GNLLLIKFEVASALEVYRVTGGRSFYRISTETPSLPAEQIQTLREAKRSVLYERQQPL
                     NVGWDDLDQTAIAGLTARIQDSRPPEAVLARTYHLVDTSRRPPTPNMAGLLLFAKDPA
                     FWHPRCGIDFVKYEGTERQHGASLNVVKRIRFEAPLVRLIDEAVGRIKEHVRERTVLH
                     DLFFRERLEYPTFAWQEALVNAVAHRDYGITGAAIEIWMFDDRIEVRSPGLPPPPVTL
                     EQLRQHKSIHFSRNPLIVRVLADLGYLKEMGEGIPRIFQEMEHYGLRSPEFSTEGFLF
                     IVTLRNTPVYDEETLRWLNQFPAAQINFRQRRLLAYAFCHGKTFSTAEYERIAEVDRD
                     AAYREIRSMVKNGIVAPLKPKSRSYRIIERL"
     gene            2015..3004
                     /locus_tag="VFN58_02305"
     CDS             2015..3004
                     /locus_tag="VFN58_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8916852.1"
                     /translation="MDFTELMRLAGGHVEARLTQTALELAIFDALESSAATAEAVANR
                     LKLEPMATELLLNSLASLGLLNKRAECFSLTAAAAKYLLKSSPQYLGAMIRFESSLWS
                     CWEKLPEAIRSGQPVRPPNMYQDDPTETEIFIKAMDSLVKARGDAEVVANAIDWSGMT
                     ELLDIGSGPASYPIAFCERFPNLHAKIFDLPGTLAITERYVREAGMTERIRLIAGDYR
                     KDPIPGDYNVIFLSNIIHGESFEINRSLIRKLVSNLKLGGQIVVKDHILDDSRTSPPV
                     GAIFSLLMLLTTESGRCYSFNEIKSWMDHAGLSRVERLDLPAPLTSSLVIGVK"
     gene            3021..3377
                     /locus_tag="VFN58_02310"
     CDS             3021..3377
                     /locus_tag="VFN58_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8916853.1"
                     /translation="MCYTAEVFRAKEGHLAIDELEQRARAQAVIENLKPACLCNKIRK
                     GTVLKAIQAGAKTFEQVSKRTGVGTGPCGGRRCGSMVRGMLGEPVISCNECGWPVLAG
                     ASPQICPRCEYAKQEV"
     gene            3421..4428
                     /locus_tag="VFN58_02315"
     CDS             3421..4428
                     /locus_tag="VFN58_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916854.1"
                     /translation="MNNRRHQTLILLLCALASMSLALPHALAQAPSEKLRVAYTVIAP
                     TQASVWTAKEMGYYAKHGLDVELVLLVGAPLAVAALVGGETPIVHTGASAVITSNLAG
                     SGAVIIAGAANRFPYVLFVTDQIKKVEELKGKKFGVSRIGSADNAAAATVLEKYGIKE
                     SDVTYVQAGSIPARLAAMQTNALQATLLQAPETLKAKELGLRALLDFTKLDVEWQQNG
                     VATTRDYIKKKPDTVRRFMRAYVEAVHYNLTNPKGAQKILQKYLAIKDDKSIEEAYNE
                     IVFKLTRRVPYPTEPGIQLFLDQLKIKNSKAGLVKPSEFTDISFLKELESSGYIDRMY
                     K"
     gene            complement(4709..5173)
                     /locus_tag="VFN58_02320"
     CDS             complement(4709..5173)
                     /locus_tag="VFN58_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET8916855.1"
                     /translation="MNLPLPEADAATAAEVRAWLDRFAACVREVDYAAARPFWHPDIV
                     IFGTYQELVKSLSAWTERQWDNVWPRTAEFRFDLANTMIMASPDGAMAVAIAPWTSTG
                     FYEDGMPFDRPGRATIVLARQPDGRWLGVHSHMSLARGVPQDSYGKRTVKTR"
     gene            complement(5256..>6062)
                     /locus_tag="VFN58_02325"
     CDS             complement(5256..>6062)
                     /locus_tag="VFN58_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916856.1"
                     /translation="ETSHPLRQLGEFLLIQLGVTSPMLLVGVLLISEIPVSLSMKDRR
                     MKWRFCFLFSAPLLVFFLFYSTRSLVKANWTLPGYLSLLIAAYPAYRYLRFNSGARMK
                     LVARYFLLAWFYALPITYLVAVYHSTVTIPNVTVHHWTTGWKELGKVVDREAAAFEAE
                     SGRKVFILGMDTYYLAAALSFYTDDTNEVFSRNLVGRSARAFDYWTPKIDPVGLNALA
                     VDLQAPDLEKLAKYFTRVDENVKRIPVMKGDRVLYRFYLVKCFGYVGSRS"
BASE COUNT         1439 a         1748 c         1634 g         1241 t
ORIGIN      
        1 gaccatgaga ccactgccaa tatcggcgtg atcgagggtg gtatggccgt caacatcgtc
       61 tcgaactcgg tggtcctccg gggtgaagca aggagtcaca gcgaggaaaa attgcagcgc
      121 cagacgcagc atatgttgca gtgtctgcac gaggcggcca gccggcacgt cttggaattg
      181 gacggcaagc gctttcaggc taaggtcgaa gccaaaatcg atcgtgacta cgaccatatg
      241 catgttcctg atgactctgc gattgttcga cttgtgcaga gggcagcgaa aaatctcgat
      301 ctcgaattga agaccctcgc cactgggggc ggctgtgacg ccaacgtgct gaataaaaaa
      361 gggctggagg tcgccaactt atccaccggc atgcgagaaa ttcatacggt caacgaatgg
      421 ctggatctca aagatctcta tctttccgct gaggtcgtat tagagactgt caggctcaac
      481 ggcgcaagcc attgaggggg atcaacgctg ttgtccttcc gctgagtggg tcactcgact
      541 ccaacgagcc ggcaccatgg acgctgaaga aatcggactc cttctcaagc aggaacgtgg
      601 acaatttctg gaattcgtaa gcgcttacga atatcggcgg ggcggaaccc aaaagaaaag
      661 agatgaagat ctcgcgcgtg aaatagtccg cgttctctcc ggcatggcca atgccgacgg
      721 cggaacgctt ctggtcggtg tcgaacccga ccgcagcgtc acgggtcttc ctcacaaccc
      781 caacgaagtc caaagcctga tccaggcccc tcaaacattg ctcactcccc cactcagcct
      841 ggcatcggca aaaattcgac tcggcaatct tctgctcatc aaattcgaag tggcttccgc
      901 gcttgaagtc tatcgtgtca cgggtggacg ttcgttttat cgtatctcga ctgagacacc
      961 ctcgcttccc gcggaacaaa ttcagactct cagagaagcc aagcgcagcg tgctttacga
     1021 aaggcagcaa cctttgaatg tcggatggga cgacctggac cagactgcca ttgccggttt
     1081 gaccgcccgg attcaagact ccaggccacc cgaggcggtt ctcgcccgca cctatcatct
     1141 ggtggatacc tcccggcgtc caccgactcc caacatggcc ggtctgttgc tttttgccaa
     1201 ggatccggct ttttggcatc ctcgttgcgg catcgacttc gtcaaatacg aaggcaccga
     1261 gaggcagcac ggcgcgtctc taaatgtggt caaacgcatt cggttcgaag caccgctggt
     1321 gcggctcatc gatgaagctg tgggccgaat caaggaacat gtccgggagc ggaccgtgct
     1381 gcacgacctg ttttttcggg agcggctcga gtatcccact tttgcttggc aggaggcttt
     1441 ggtcaacgcg gtagcgcacc gggattatgg catcaccggc gcggccatcg agatctggat
     1501 gttcgatgat cgaatcgaag tgcgcagccc cggtcttccg cccccgcccg taaccctcga
     1561 acaattgcgt cagcacaaga gcattcactt ttcccgcaac ccactgatcg tacgtgtcct
     1621 agcggatctc ggttatctga aggaaatggg cgaaggcata ccgaggattt tccaggagat
     1681 ggagcactac ggcctccgct ctccggaatt ttccaccgaa ggatttcttt tcattgtcac
     1741 tttgcgcaac acacccgtct atgacgaaga gaccctgcgc tggctcaacc aattcccggc
     1801 ggctcagatc aactttcggc aacggcggct cctggcctac gctttctgtc acggcaagac
     1861 cttctccact gccgaatatg aacgcatcgc cgaagtcgat cgcgacgccg cctaccggga
     1921 gattcgctcg atggtgaaaa acggcatcgt cgcgccgctg aaaccaaaga gccggagcta
     1981 tcggatcatc gaaaggctct aaccacgagg cccgatggat ttcacggaac tcatgcgatt
     2041 ggccggcggt cacgtcgaag cgcggctcac gcaaaccgct ctggaattgg cgattttcga
     2101 tgctctggaa agctccgcag cgaccgccga agcggtggcc aatcgactca agctcgagcc
     2161 gatggcaacg gaactgctgc tgaactctct ggcgtccttg ggactgctaa acaaacgcgc
     2221 ggaatgtttt tctctgacag cagccgcggc aaaatatctg ctcaagagtt caccgcaata
     2281 tctcggcgcc atgatccgtt tcgaatcgtc gctctggagt tgctgggaaa aactccccga
     2341 ggccattcgc tccggtcaac cggttcgtcc cccgaacatg taccaagacg atcccacaga
     2401 aaccgagatc ttcatcaaag caatggactc tctggtcaag gcgcgcggcg acgcggaagt
     2461 ggtggcgaac gctattgatt ggagcggaat gactgagctg cttgacatcg gctccggccc
     2521 cgcaagctat ccgattgcgt tttgcgagcg ttttcccaac cttcacgcca aaatcttcga
     2581 tctacccggc acgcttgcta tcaccgaaag atatgttcgg gaagcgggta tgacagaacg
     2641 aatccggctc atcgccggcg actatcggaa agacccgatc cctggagact acaacgtcat
     2701 ttttctctcc aacatcatcc atggcgaaag ctttgaaatt aaccgatcgc tgatccgcaa
     2761 acttgtctct aaccttaaac tcggcgggca aattgtcgtg aaagatcaca tcctcgacga
     2821 tagtcgtact agcccgcctg tgggcgccat ctttagtttg ctcatgttgc tcacgaccga
     2881 atcagggcgc tgttatagct ttaacgaaat caaaagctgg atggaccacg ccggtctaag
     2941 tcgcgtagag cgcctcgatc ttccggcacc gctaacctct tcgctcgtca tcggcgtcaa
     3001 gtaattagct tggcgcaaaa ttgtgctaca ctgctgaggt attcagagca aaggaagggc
     3061 acctcgccat cgacgagcta gaacaaagag ccagagcgca ggcagtcatt gagaacctca
     3121 aaccggcctg cctctgcaac aagattcgca aaggcaccgt gctcaaagcc atccaggctg
     3181 gggctaagac cttcgagcaa gtgagcaaac gcaccggcgt tggcaccggc ccgtgcggcg
     3241 gcaggcgctg cggctcaatg gttcgaggga tgctgggaga gcctgtgata agttgcaacg
     3301 aatgtggctg gccggtgtta gcaggggcct caccacaaat ttgcccgcgc tgtgaatatg
     3361 ccaaacagga agtctgactc gccgtttttg actagtgaat ccagtttcag gaggacgggc
     3421 atgaataacc gacggcatca aacattgatt ttacttttat gcgcgcttgc atcaatgtcg
     3481 ctggcgctgc cccacgcttt ggcgcaagcc ccgtcggaaa aacttcgcgt tgcgtacaca
     3541 gtcatcgcac cgactcaagc aagcgtctgg accgctaaag agatgggcta ctatgcaaag
     3601 cacggtctcg acgtcgagtt ggttctcctc gtcggcgcgc cgctggctgt ggccgcgctg
     3661 gtgggtgggg aaacaccgat cgttcacact ggcgcttccg cggtgatcac cagcaaccta
     3721 gccggctccg gagccgtgat tattgccggc gcggccaatc gttttcctta cgtccttttt
     3781 gttacagacc aaatcaagaa agttgaggaa ttgaaaggga agaagttcgg cgtcagccgc
     3841 atcggcagcg ccgacaacgc cgccgccgcc acggtcttag aaaaatacgg catcaaagaa
     3901 agcgatgtga cctatgttca ggccggctcg attccggccc ggctcgctgc gatgcaaacg
     3961 aatgcgctcc aggcgaccct gctgcaagcg ccggaaacgc tcaaagccaa ggagttgggt
     4021 ctccgcgcgt tgctcgactt caccaagctg gatgtcgaat ggcagcaaaa tggggtcgcc
     4081 accacgcgcg actacatcaa gaaaaaaccc gacaccgtgc gccggttcat gcgagcctat
     4141 gttgaagcgg tccactataa tcttaccaat ccgaagggcg cgcaaaagat tctacaaaag
     4201 tatctcgcga tcaaagacga caaatccatc gaagaggcct acaacgaaat cgtcttcaag
     4261 ctgacgcgcc gggtccccta tccgacggaa cccggcatcc aattgtttct agaccagttg
     4321 aaaataaaaa attccaaagc cggactggtc aaaccaagtg agtttaccga catcagtttc
     4381 ctaaaagagc tcgagagcag cggttacatc gaccgtatgt ataagtgaca ccgacgctcc
     4441 aatggattgt tagcccagac tattcatgct ccggaaaaac cacgacacag cgtcgatcaa
     4501 gggatgagct cggaggagcg aaggcacaga gacgaacagc ttcaatgacg tttatgatgc
     4561 ttggctgtac gctcgaattg ttctcaggca tccgcttcag aacttgggtt tggagaccgc
     4621 ctcaaggtag aggcctattg tcttcgatcc ggagagctcg tcccttgatc gttgcttgcc
     4681 agtgaataat ccgggttagc actctcacct accgcgtctt caccgtcctt ttcccatagc
     4741 tatcctgcgg cacgccgcgc gccagcgaca tgtgcgaatg cacgccgagc cagcgaccgt
     4801 ccggctgtct cgccagcaca atcgtggcac ggcccgggcg atcgaagggc attccgtcct
     4861 catagaaacc ggtgctggtc cagggcgcga tcgcaaccgc catcgcgcca tccggcgagg
     4921 ccatgatcat ggtgttcgcc aggtcgaatc gaaattccgc cgtgcgcggc cagacattgt
     4981 cccattgccg ctcggtccag gccgataggc tcttcaccag ctcctggtag gtgccgaaga
     5041 tgacaatatc ggggtgccag aacggacgcg ccgccgcgta gtctacctcc cgcacgcagg
     5101 cggcgaagcg gtctagccag gcgcgcacct ccgccgcagt ggcggcatcg gcctccggaa
     5161 ggggtaggtt catcttggac ctctcgtcat ctcgcctatc gtcaacagat ccgctcgcct
     5221 tgatcgcgac ctttcagccg cttgctcgag cccaactaag atcggctccc gacataacca
     5281 aagcacttca ccagatagaa acggtaaaga accctatcac ccttcatgac gggtatccgt
     5341 tttacgtttt cgtcaacccg cgtaaagtat tttgccaatt tctcaaggtc tggagcctga
     5401 aggtccaccg ccaacgcatt gagacccacc gggtcgatct ttggtgtcca gtaatcgaag
     5461 gcccgggcgc ttcttccgac gaggtttcgc gagaaaactt cgttggtgtc atcggtgtag
     5521 aacgagaggg cggcagccag ataataggtg tccatgccga ggataaaaac cttcctgccg
     5581 gattcggctt caaaagctgc tgcctcccta tcgacgacct tacctaactc tttccaaccg
     5641 gtcgtccagt ggtgcacggt gacgttagga atcgttacag tcgagtggta aaccgcaacg
     5701 agataggtaa tcggcaaagc atagaaccaa gctaacaaga aatatcgggc gacgagcttc
     5761 atccgggctc cgctgttgaa ccgaagatag cgataggcag gataggcggc gataagcaga
     5821 gagagatatc cgggaagcgt ccagttggcc ttgaccaaag agcgagtact ataaaacagg
     5881 aaaaagacga gcagtggcgc cgaaaagaga aaacagaatc tccacttcat acgccggtct
     5941 ttcatgctta gggagacggg aatctctgaa atgagcagga ccccgaccaa aagcatcgga
     6001 gaagtcaccc cgagctgtat gagtagaaat tcccctaact gccgcagcgg atggctagtc
     6061 tc
//
LOCUS       DASSWZ010000091         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_752572, whole genome shotgun sequence.
ACCESSION   DASSWZ010000091 DASSWZ010000000
VERSION     DASSWZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752572"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..454
                     /locus_tag="VFN58_02330"
     CDS             152..454
                     /locus_tag="VFN58_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916857.1"
                     /translation="MNNIKPLMSVVFLTGALGLSVASAGAEAVISKDVLADGSYCHTK
                     FESIREDTLASSQPVLKDTDDIIDFYGACNHDPLGKEEIQSQKLEMQHRFQSEYND"
     gene            692..1171
                     /locus_tag="VFN58_02335"
     CDS             692..1171
                     /locus_tag="VFN58_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8916858.1"
                     /translation="MKSLFTVITLVLGLVFMQAQAFAALPKAKPESPNVAAGREAMEA
                     KDFKSAVGNLAKAVLENPNDADAHVMLGYSYRKLGSFDKSMEHYQKALKIDSSHRSAH
                     EYLGELYLEMHQPANAEEQLQVLKKACPFFGKCAEYDDLKEAINKYRTNTSRSPAEL"
     gene            1272..>2288
                     /locus_tag="VFN58_02340"
     CDS             1272..>2288
                     /locus_tag="VFN58_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HET8916859.1"
                     /translation="MITHNIQIAKGFRWLGSLMGVSFLALAACTQMVQPAASTQPAKA
                     YVSNFKDNTVSVIDTDAGKVVATVPVAAGPHGMAITQDGRTVYISGDGSSSLDVIDTA
                     TDKVVKTINVGKTPNGIALTPDNKLLLVTVYGENRLAFVDTATQTVVATVAVPKPHTV
                     AISSDGKLAYVTAQEPGHFELDLIDLNKRSVVRTLSLEKTPRDGEFGYDGKLFYFTQA
                     GVSSVQVLDPASNKIVAEIPTGVSPHFVNYPRGSAFGIVVVQGPGELLLFDPATNKPA
                     RSVAVGKQPNWAATSGDGKTVYVTNEGSNDVTVVDLATGRTATIPVGNAPRRVVVQQT
                     AATAG"
BASE COUNT          563 a          591 c          639 g          495 t
ORIGIN      
        1 gagtgggtgt gagtacatag tttttcctct gcgcctgctc atggagtaac tctgtgaaaa
       61 gcaattttca ggactcttta aggaattttt cctgaagact tgagtctcat atagcagagg
      121 ccgcgagttg gccggaaagg aggtgataaa catgaataac atcaagccgc ttatgtctgt
      181 tgtgtttctg acgggagctt tgggcttgag tgtagcgtca gcaggggctg aagctgtcat
      241 ctccaaagat gtcttagccg acggcagtta ctgccacacg aagttcgaga gcattcgtga
      301 agacactttg gcttcgtctc aaccggtcct taaggatacc gatgacatca tcgatttcta
      361 tggtgcttgc aaccatgatc ctctggggaa ggaagagatt cagtctcaga agcttgagat
      421 gcagcacagg ttccagagcg agtacaacga ttgaggattt tttgatcctg ctcctggggg
      481 caacaggcag caacctgttg cccctggtgg gcaccgatcc tcggcacacc gccgtgacga
      541 gtcggagatt agttttcggg gaataaactc agattgaaat agtcataaag gttagaaagg
      601 acggacgata aagatttcag gtgtgtaaca ggcacgctgg attaattcaa taggctcacc
      661 cgtttgtcca tctacaaagg aggtctacga tatgaaatcg ttatttactg ttattacttt
      721 ggttcttggt ttggtgttca tgcaagctca ggctttcgcc gctctaccaa aggcgaagcc
      781 tgaaagtcca aatgtcgccg ccggacggga agccatggag gcgaaggact tcaagtctgc
      841 agtgggaaat ttggcgaaag ccgttctgga aaacccgaac gacgccgatg ctcacgtcat
      901 gctcggctac agctatcgca aactcggctc cttcgacaaa tccatggagc attaccagaa
      961 ggctctgaag atagattcca gtcatcggag cgcacacgaa tatcttggcg agctttacct
     1021 cgagatgcat caacctgcca acgccgagga gcaactccag gtactgaaaa aggcttgtcc
     1081 cttctttgga aaatgcgcgg agtacgacga cctcaaggaa gcaatcaata agtacagaac
     1141 gaatacttct cgttcgccag cagagctcta aggagaatcc ttacgggccg gtccacttcc
     1201 cacgggaata tttattcaaa tgaaagagtc atacagatat gcagaagaac tttataagga
     1261 ggtactaaga aatgataacg cataatatac agatcgctaa agggtttcgg tggctaggtt
     1321 ccttgatggg agttagcttc cttgcgctgg ctgcctgcac ccagatggtg cagcctgccg
     1381 cttccactca gccggccaag gcctacgtga gcaatttcaa agataacacg gtgagtgtga
     1441 tcgataccga tgctggcaag gtcgtcgcga cggtgccggt agccgcgggt ccgcacggca
     1501 tggcgataac gcaggacggc cgcaccgtct atataagcgg cgatggctct tcgtcgcttg
     1561 acgtcatcga tacggccaca gacaaagtcg tgaagaccat caacgtcggc aagacaccga
     1621 acggtatagc actcacaccc gacaacaaac tgctgctcgt cacggtctac ggtgagaacc
     1681 gtcttgcctt cgtcgataca gcgacacaga ccgtggttgc gacggtcgcg gtgcctaagc
     1741 cgcatactgt cgcgatcagc tccgacggca agctcgcgta cgtcacggcg caggaaccgg
     1801 gccacttcga gcttgacctc atcgacctga ataagcgcag cgtggttcgc acgctatcgc
     1861 tcgagaagac accgcgcgac ggggagttcg gttacgacgg caagttgttc tactttaccc
     1921 aagccggagt cagttcagtc caggtgctcg atccggcgtc caacaagatc gtggcggaga
     1981 ttccgacggg cgtatcgccc catttcgtta attacccgcg cggctcggcg ttcggcatag
     2041 tagtcgtgca ggggccgggg gagctactct tgttcgaccc agcgacgaac aagccggcgc
     2101 gcagcgtcgc ggtcgggaaa cagccaaact gggcggccac gtccggcgac ggtaaaaccg
     2161 tgtacgtaac caatgagggc tcgaacgatg tcaccgtcgt cgatctcgca actggccgaa
     2221 ctgcgacgat cccggtcggc aacgcgccac gcagagttgt tgtgcagcaa accgcggcga
     2281 ccgctggg
//
LOCUS       DASSWZ010000092         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_398674, whole genome shotgun sequence.
ACCESSION   DASSWZ010000092 DASSWZ010000000
VERSION     DASSWZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398674"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFN58_02345"
     CDS             complement(<1..278)
                     /locus_tag="VFN58_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916860.1"
                     /translation="MKLQAAEIRRSSRSFFERGHRKRLGVASRHLVEATAALLCLRFV
                     TAFLIELTPQEAYYWNYAVHPALSYFDHPPMVAWVIGAGYFLLGKCELG"
     gene            567..914
                     /locus_tag="VFN58_02350"
     CDS             567..914
                     /locus_tag="VFN58_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET8916861.1"
                     /translation="MRLASGIQDETAVRQVFTEIQNRLAQDGKAEAMDGVLVQPMISG
                     VELVIGVTQDPLFGPLIGFGLGGIHVEILKDVCFRITPITDRGTREMIRSIKGYRLLQ
                     GYRGHPPADIEAL"
     gene            943..1071
                     /locus_tag="VFN58_02355"
     CDS             943..1071
                     /locus_tag="VFN58_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916862.1"
                     /translation="MEEVPEIVELDLNPVMALPPGRGCLIVDARIALKAGENGLSA"
     gene            complement(1120..1797)
                     /locus_tag="VFN58_02360"
     CDS             complement(1120..1797)
                     /locus_tag="VFN58_02360"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HET8916863.1"
                     /translation="MKSHITLALFFFTLLVSGTAFAAENAASTADKSEPKPWVRKVVF
                     GILAHDIGPLADSQESLAPDPNWEVQFNPPEWRWWRWVGSPSPMTGAIPNFTGGTSAF
                     YLGLAWELSLSYEFLNNLTNDFTRRMWVSGGWGPAIHNGPLTKNTADCRSREGSLKTT
                     GECGFGTRVLPMLQIEIGATFWKNHALSLYGDHMSGGAVFGGAQNEGLDHVGLRYHFF
                     FNKNGTP"
BASE COUNT          470 a          554 c          554 g          434 t
ORIGIN      
        1 ccgagttcac acttgccgag cagaaaataa ccggcgccaa tcacccaggc aaccatcggt
       61 ggatggtcga aatagctgag cgcgggatgc acggcgtaat tccaatagta ggcctcttgc
      121 ggtgtgagct cgatcagaaa agcagtcaca aagcggagac agagaagcgc ggcagtcgct
      181 tcgacgagat gtctactagc tacgccgagt cgtttcctat gacctctctc gaaaaacgat
      241 cgactcgatc ttcgaatttc ggcagcctgc agcttcattc tagcctcatg aattgcgcgc
      301 agacctccgc cgagaagggg acatagatcc gcagcatgta caaaactgtt gtggcaaaaa
      361 gacgcccttg attcaacccg gcttccgcag ttgggattga cagagacgcg aggcacaacc
      421 cttccgcagc gcagtcgatg ggttgctgcc tcggagtaga catctctcag cggtgcttcg
      481 agggcaggtt agcctcttag caacgacgtg cgaaatcgca aaatgacttt ggaatttcac
      541 agtcggacgc cttcgctgct ccaggattgc gcctcgcttc aggcattcag gacgagacgg
      601 ccgtccgcca agtattcacc gagatccaaa atcgacttgc gcaagacggc aaagcggagg
      661 cgatggacgg cgtgttggta caacccatga ttagcggcgt cgagctcgtc atcggcgtca
      721 cccaggatcc tctcttcggc ccgctcatcg gctttgggtt gggcggcatt cacgtggaga
      781 ttctcaaaga cgtttgcttc cgcattaccc ccatcacaga tcgcggcacc agggaaatga
      841 tacggagcat taaaggctat cgtctcctgc agggttatcg cggtcacccg ccggcggaca
      901 tcgaggccct ttgaagatct cctgcttcgc atctcccggt tggtggaaga ggttccggag
      961 attgtcgagc tagacttaaa cccggtgatg gctctgccgc cgggtcgcgg ctgtttgatt
     1021 gttgacgcaa ggatcgcgct caaggcgggg gaaaacggtt tgagcgctta ggcaggctct
     1081 ccaaaattcc caggcaagct ctcttacgtg gcgtgccgat catggggttc cattcttgtt
     1141 gaaaaagaaa tgatagcgaa gcccaacgtg atcgagtccc tcattctgcg ctcctccgaa
     1201 aactgcgccg cccgacatgt gatccccgta aagtgacagc gcgtgatttt tccagaaagt
     1261 ggctcctatt tctatctgga gcattggcag aactcgagtc ccaaatccgc attctcccgt
     1321 cgttttgaga gaaccttctc ttgacctgca gtcggccgtg ttttttgtca gcgggccgtt
     1381 gtgaatcgcc ggaccccaac cgcctgagac ccacattctc ctggtgaaat cattggtgag
     1441 gttgttcagg aactcgtagg acaaactcag ctcccaggcg agtccgagat aaaaagcgct
     1501 cgtgcctccg gtaaagttcg gaatggcgcc ggtcataggg gagggggagc ccacccaacg
     1561 ccaccacctc cactcgggcg gattgaactg cacctcccaa ttgggatcag gagccaggct
     1621 ctcctgactg tcggccagcg gcccgatatc atgcgcgagg atgccgaaca caactttccg
     1681 gacccagggt tttggctcgc tcttatccgc ggtactcgct gcgttttccg cggcgaacgc
     1741 ggttcctgaa acaagaagag taaaaaaaaa caacgccaga gtgatgtgcg atttcatgtg
     1801 agacccaaga ataacatcag gaaacagttt tctgcaagga ctaagcgcag ttttgagccg
     1861 taatgaatgg gtcttctggg tttagtgtgg aagagcattc accacaaagt gactcatgat
     1921 cttacgcgcg cccgtagaac ctgaaaatag atagtttccg cgtcccggcc ctttagggag
     1981 agtccctgag ttcaaatcag gaaaaacgga ag
//
LOCUS       DASSWZ010000093         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1389475, whole genome shotgun sequence.
ACCESSION   DASSWZ010000093 DASSWZ010000000
VERSION     DASSWZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389475"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /gene="yfbR"
                     /locus_tag="VFN58_02365"
     CDS             <1..345
                     /gene="yfbR"
                     /locus_tag="VFN58_02365"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: protein motif:HMM:NF003009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HET8916864.1"
                     /translation="PVKYFNPEIKNAYKEIEAAAASRLLKMIPEGLKADYQGLFFAGE
                     ADQESRELVKAADKLCAYLKCLEEIGAGNQEFAKAEKALRASVDELKLPEVRYFLDTF
                     VPSFRLTLDELD"
     gene            833..1624
                     /locus_tag="VFN58_02370"
     CDS             833..1624
                     /locus_tag="VFN58_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET8916865.1"
                     /translation="MNTIKTAISGHKVVSRKEWLEARKKLLKKEKESTRLRDQLSAER
                     RKLPWVKVEKQYVFDAPQGKVTLADLFDGRSQLVIYHFMFGPDWKEGCPSCSFTSDHI
                     DGTLAHLAARDVTIVIVSRAPLGKIEAFKKRMGWRFKWVSSYGSDFNPDFRVSFTKDE
                     MAQGKVNYNYTTQEFPSEEAPGVSVFYRDPGGDLFHTYSTYGRGVEQLVGTYMILDMV
                     PKGRDEDHLGFTMEWVRHHDRYGTDEFADANKPYWPATTSPSSTS"
     gene            1760..4405
                     /locus_tag="VFN58_02375"
     CDS             1760..4405
                     /locus_tag="VFN58_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET8916866.1"
                     /translation="MSSIESSDILTSMRSQEFGDQNPEALRDQYQALLEVTESIASHR
                     DLSELFHDLAQPLRNVLHFDYLSVRLHDPQRKVMRIHILEKSAPGELVPELPVNESLA
                     GFVWQTQQALLIADIEHEVRFPRAMQRLRENHIKSCCCLPLSTAHRRLGAMTLGSVRD
                     KAYQPTEFRFLEQVARQVAMAIDNALNFEQAQSLQKQFKGERDHLSLLLEVNNALVST
                     LNLSELLNAVSASLRPLVPHEYASLSLYDPKTGCLQVHALDFPVDNKLYEECLSVPVE
                     GTPTGLAYSSRKPVVLNREGLEGFPSDICRLLLAKKVQSVCCLPLISHNRALGALNLA
                     SLQENAFGPEDVQLLNEVASQVAIAVENALNFEQAQSVQQQLKEEHDRLRLLLDVNNT
                     IVSSLDLRELLNAVSASLRRLVPHEYASLSLYDAQTQRLQIHALDFPASKGLIQEGLW
                     IPVEGTPHGRALTSRQPLFVTLQEIEEFELTPRIRAEGLKSGCFLPLISHGRPLGTLV
                     VASLQAESFPQKDADLLQHVANQIAIGVENALAYGQVVDRANKLSEEKLYLQDEIRTE
                     HNFEEIIGESPALKKVLEQIQTVAPTDSTILILGETGTGKELIARAIHNLSTRRERTL
                     VKVNCSAIPTGLLESELFGHEKGAFTGAIAQRIGRFELAHRGTIFLDEVGDISLELQP
                     KLLRVLQEQEFERLGSTKTIRVDVRMVAATNADLAQKVADNQFRLDLYYRLNVFPVVI
                     PPLRERREDIPLLVRYFAQKYARRMKKPIDTIPMKTMSALVDYHWPGNVRELENFIER
                     AVILSRSADLKIPVAELKQRGRVTGAASPNGLGTLEHAEREHISRALSESNWVIGGPA
                     GAAFKLGMKRTTLQSKMRKLGIARP"
     gene            complement(4643..>4892)
                     /locus_tag="VFN58_02380"
     CDS             complement(4643..>4892)
                     /locus_tag="VFN58_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916867.1"
                     /translation="FTATLGAINTAIGADGILSKDQLTAVVTVTRSFRDQPEHLGDSQ
                     PALKDSSTGDVIDFYGPCNEDPVGTDQVQEQKLKPSID"
BASE COUNT         1150 a         1337 c         1355 g         1050 t
ORIGIN      
        1 cccgtcaagt acttcaatcc tgagatcaag aatgcttaca aagaaatcga agccgctgcg
       61 gccagtaggc tgcttaagat gattccggaa ggtttgaaag cagattatca ggggctcttt
      121 ttcgctggcg aagcggatca ggagtctcgc gaactcgtca aggcagcgga caaactctgt
      181 gcttacctca agtgcttgga agagatcggc gcgggaaatc aggaattcgc gaaagctgag
      241 aaggcgctta gagcgagcgt cgatgagcta aagcttccgg aggtacgcta ctttctggac
      301 acgtttgtgc cgagcttcag attgactctt gacgaactcg attgaagttg cgagctcaaa
      361 aaactcaatc tggagccttc gagctcatgc ttcaagggtt tctgaagatc aactcgtagg
      421 ttggctgaac atgagaggaa gaacggcata cttattgact acatcggaaa gccgttagca
      481 ataccaccgg cacgctccgg gcggattttt gtcggcgatt gggaggacca ccgaagcacg
      541 agcctcctat aaacgggcgc tcgcgcgaca ggagccggag cggtggtttc tgggactgcg
      601 gctccgttaa ttggggcgag atgaaatcgc gctgtcgatt ttggctcccc ccaaacgact
      661 atccgctgaa agcacagcga gcctgacatc ccctagtcct cgggatgtct gtcgcgactt
      721 ctggacctga aaaccttgtg gcaacgggct tacccaaatg tcgatttggg gctccgccaa
      781 acgactatcc agtggaaaga atagttcaac ccagaaaaaa ggaggtcaaa taatgaatac
      841 aatcaagaca gcgatatcag gccacaaggt cgtatcgagg aaggaatggc tcgaggcgcg
      901 caaaaaactt ttgaaaaaag agaaggaatc cacccggctg cgcgaccagc tgagcgccga
      961 acggcggaaa cttccgtggg tgaaggtcga gaagcagtac gtgttcgatg ccccacaggg
     1021 caaggtgacg ctcgccgacc tgttcgacgg ccgcagccaa ttggtcatct accacttcat
     1081 gttcggaccg gattggaagg agggctgccc tagctgctcg tttacgtccg accatatcga
     1141 cggcacacta gcgcatttgg cggcacgcga cgtaacgata gtgatagtct cgcgggcgcc
     1201 gctgggcaag atcgaggcat tcaagaaacg catgggctgg cggttcaagt gggtgtcgtc
     1261 atacggcagc gacttcaacc ctgacttccg cgtttcattc acgaaggacg agatggcgca
     1321 gggcaaggtg aactacaact atacgacgca ggagttcccc agcgaggagg cgccaggcgt
     1381 cagcgtcttc tacagggacc ccggtgggga cctcttccac acgtactcca cctacgggcg
     1441 cggcgtcgag caactcgtcg gcacgtacat gattctcgac atggtgccca agggccgcga
     1501 cgaggaccat ctgggattca cgatggaatg ggtccgccac catgatcgtt acggtaccga
     1561 cgagttcgcg gacgcgaaca aaccctactg gccggcgacc acgtcgccgt cgtccacgag
     1621 ctgatcatgc ggatcgaacc gaactgtgtc aagtcggcga ccatttgaac cagttgtgat
     1681 tgcaaatact tttcacgaat ggaaagacgg acacgatcgt gtctgtctcc gaagcgatga
     1741 tgaaagacaa ttgcgtagct tgagtagcat tgaatcgagc gatattctta catccatgcg
     1801 tagtcaagaa tttggggatc agaaccccga ggcgttgcgg gatcagtatc aagccctcct
     1861 agaggtcacg gagtctatcg cctcccatcg agacctatcc gaattgtttc acgatctcgc
     1921 ccagccgctg cgcaatgtgc ttcacttcga ttatttgagt gtgcggctgc atgatccgca
     1981 gcgcaaagta atgcgcatac acattttgga aaaatccgcg cccggagagt tagttcctga
     2041 attacccgtc aacgaatcgc tcgcgggttt cgtatggcaa actcagcaag ctctccttat
     2101 cgccgacatt gagcacgagg ttcggttccc acgcgcgatg cagaggttgc gcgaaaatca
     2161 catcaaatcc tgctgctgtt tgccgttgag caccgcgcac agacgcctgg gagcgatgac
     2221 cttaggcagc gttagagata aagcgtatca gcccacggaa tttcggtttc tcgagcaggt
     2281 tgcaaggcaa gtggcgatgg ccatcgacaa cgcgcttaat tttgagcagg ctcagtcgct
     2341 tcagaagcaa ttcaaggggg agcgtgacca tttgagcctg ttgctagagg ttaacaacgc
     2401 cctggtctcg accctcaact tgagcgagct gcttaacgcg gtatccgcat cgctgcgccc
     2461 tctggtgcca catgaatatg cgagcctatc cctctatgat cctaaaaccg gatgtctgca
     2521 ggtccatgcc ctggactttc cggtcgataa taaactttat gaggaatgtc tatcggtccc
     2581 cgtcgagggt acgccgacag gcctggctta ttcgtcgcgc aaacctgtgg tgctgaaccg
     2641 cgagggtctt gagggtttcc cgagtgatat ttgtcgcttg ttattggcga agaaagttca
     2701 atcggtctgt tgcttaccgc tgatttcgca caaccgtgcc cttggcgctt tgaacttggc
     2761 gagcttgcag gaaaatgcgt tcgggccgga agacgtccag cttctaaacg aagtcgcctc
     2821 ccaggttgcg atcgccgtcg agaacgcgct caacttcgag caggcacaat cggtgcagca
     2881 acaactcaag gaggagcacg atcgcttgag gctcttgctg gatgtcaaca atacaatcgt
     2941 ctcatcgctg gatttacgcg aactgctcaa tgccgtctcc gcgtcgctgc ggcgcctggt
     3001 gccgcacgaa tatgccagcc tgtcgctcta tgacgcccaa actcagcgct tgcagattca
     3061 cgcgttggat tttcccgcca gcaagggctt gatccaggaa ggcctctgga tccctgtaga
     3121 gggtacgcct cacggccgag ccttaacctc acgtcaaccg ctgttcgtga ccctccagga
     3181 gattgaggaa tttgagctta ccccgcgcat ccgcgcggaa ggacttaagt ccggatgttt
     3241 cttgcctctc atttcgcacg gccggcccct cggcacgctc gtggtggcga gcttacaagc
     3301 ggagagtttt cctcaaaaag atgccgattt gctccagcat gtggccaatc aaatagccat
     3361 cggggtcgag aacgcgctcg cgtatggtca agtcgtggac cgggcaaaca agcttagcga
     3421 agagaagctc tacctccaag acgagatccg caccgaacac aacttcgaag agatcatcgg
     3481 tgaaagtcct gccttgaaaa aggtcctgga acagatacag acggtggcgc cgacggattc
     3541 gacgattctc atcctaggtg aaaccggcac cggcaaggag ttgatcgctc gcgccattca
     3601 caatctcagc acgcgtcgcg agcggacact cgtgaaagtg aactgttccg ctattccgac
     3661 cggactgctc gaaagcgaac ttttcggaca cgagaaggga gctttcacag gcgctattgc
     3721 gcaacgaatc ggccggttcg aactggctca ccgaggcacg atttttctcg acgaggtagg
     3781 cgatatctcc ctcgagctcc aacccaagct cctgcgcgtc ctccaggaac aagagtttga
     3841 gcggctggga agcacgaaaa cgatacgcgt ggatgttcgc atggtggcag ctaccaacgc
     3901 ggatctggcg caaaaggttg ccgacaatca atttcgtctt gacctttatt accggcttaa
     3961 tgtgttcccg gtcgtgatcc cgccgctgcg cgaacgtaga gaagatatcc ctcttctcgt
     4021 gcgttacttc gcgcagaaat atgcgcggcg catgaaaaag ccgattgata cgattccgat
     4081 gaaaacgatg agtgctttgg tggactacca ctggcctggc aatgtgcgtg agctcgaaaa
     4141 cttcatcgag cgcgcggtca tcctatcccg tagcgcggac ctaaaaattc ccgtcgctga
     4201 gttgaaacaa agggggagag tcacaggggc cgcttctccg aacggtctcg gaaccctcga
     4261 gcacgcggaa cgggagcaca tctcgcgcgc tctcagtgaa agcaattggg tgatcggcgg
     4321 acctgcgggc gccgcattca aactcggaat gaagcgcaca actctccaat ccaaaatgcg
     4381 caagctcggc attgcgcgac cttagtaagt cttgccgaca tgacggcaat gaaagggtga
     4441 cgcagggcct ggtataattg ttctcctcca gggtcttcag atgtctttgc tgaaccggga
     4501 tactgaacat ccaggctgac gctgctgttg ccaccgtgcg gccgtcgttc cggctagaca
     4561 atagcgacag aacgcgcaga ggagggcacc tgccctcccc cttgcaccgg tttcaacagc
     4621 aactaatcag agtattccat ctctaatcga tgctgggctt caatttctgc tcttggactt
     4681 gatctgttcc aaccggatcc tcgttacacg gcccgtagaa atcgatcacg tcgcctgtgc
     4741 ttgaatcttt cagagctggt tgactatctc caaggtgctc tggctgatcc cggaaacttc
     4801 tcgtgacagt aactactgca gtcaactggt ccttcgagag aatgccatcc gcaccgattg
     4861 ccgtgttaat tgctcccagt gtagctgtga ag
//
LOCUS       DASSWZ010000094         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1248101, whole genome shotgun sequence.
ACCESSION   DASSWZ010000094 DASSWZ010000000
VERSION     DASSWZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248101"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1297
                     /locus_tag="VFN58_02385"
     CDS             296..1297
                     /locus_tag="VFN58_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916868.1"
                     /translation="MKLGVTAKKPFSVKLIFLLSLALCLGFSRVSSALDEAKIHVAGT
                     PNITSFVYHVGKEKGFYREEGIELQPIMAGMLQGIQGLIGGSFDYSQMLGQGGGAILR
                     GAPLKIVMAFDTRPSWFLLGGKNMKSLQDLKGKQLAVSSFGAALDQMTRELLPRFGME
                     PMRDVVLRAIEPPPNRLAALLSGAVDAAVLTHPETIIARKQGFNELLFFGDHIEFVSA
                     GVVATERNISQKPDFVRRFIRGALKTFYWIKANEKEVVTRLAKATKSSETDGLELYRT
                     GVKLFSQDGTIPRSLQERMISMQRKVLQVEKEIAPESVYDFSFVRAANKELGKGESL"
     gene            1294..2343
                     /locus_tag="VFN58_02390"
     CDS             1294..2343
                     /locus_tag="VFN58_02390"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916869.1"
                     /translation="MSILDQSLLIANVNYHVGHWVAIYVAEEQGFFKEEGLTRYEYER
                     GGLLPGPSESQTLGLAIKEHGVDIATAVDTESAICQRLQGADVYIVGGWRYTPFLKWY
                     GSKHITDISKLRGGKIGVRESSADGGGLVEFFIEEALKEAGVNPETEVEQVQNPVFGY
                     RNNPAHIEMLRSGKVNAITSSPPFSDQLEKEGYPVILDPNKVFPRRPGKVTVAPRRTI
                     EQRGEELKAYFRAIIRTFWFMRDVKNFEYLHDLEARLRKLTHNEDERSLFIASAPDRV
                     DSWALPMDGGIERQALDRIIQEMVKRGRLERPLPVEEVFRDDAVRDAYRDVSKRAKLK
                     PAFDKAMAAVEKYGF"
     gene            complement(2419..>2648)
                     /locus_tag="VFN58_02395"
     CDS             complement(2419..>2648)
                     /locus_tag="VFN58_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HET8916870.1"
                     /translation="IGKEIEKKGIPVVVITCLSNVATQVGVGRILGGSKFHYPVGQPE
                     LPPEEELRWRVGLLEKGLAALETPVKGPKIF"
BASE COUNT          646 a          702 c          737 g          563 t
ORIGIN      
        1 tcagcaccga tattgtcttg acccgcgctt gagcggatca taagtgatga gataggacgt
       61 agactcagtc gtcggcgcta atgttcccag agttctaagt ctgtcactcc cgaatgcgtt
      121 tgtcgggaat ccaggcgaaa actgtgactg accctataat aacattcgat tcagttcgat
      181 cctaaccgga gggttcggga cgacagtttt ctcttccgct ttgcaaaaca tttggacaac
      241 gtctcgctga gatcagtttg accgcaaccg ggctaagcag gtaaaggaga cttgtatgaa
      301 gttaggagtg accgcaaaaa aaccgttcag cgttaagttg atctttcttt tgtctctggc
      361 tttatgcttg ggtttcagtc gagtctcctc ggcgttggat gaagcaaaaa ttcatgtggc
      421 cggaacgccg aacatcacga gcttcgtcta tcacgtcggc aaagaaaaag ggttttacag
      481 agaagagggg atcgagctgc aaccgatcat ggctgggatg ctccaaggaa ttcaggggct
      541 gatcggcggg agcttcgact acagccaaat gctcgggcaa ggcggaggcg ccattctgag
      601 aggcgctcca ctcaagatcg tcatggcttt tgacacgcgc ccgtcgtggt ttcttctcgg
      661 cggcaaaaat atgaaaagcc tccaagattt gaaaggcaaa caattggccg tctccagttt
      721 tggcgccgcc ctggatcaga tgacccgcga gctgctgccg agatttggca tggaaccgat
      781 gcgcgacgtc gtcctgcggg ccatcgagcc cccgcccaat aggctggctg ctctattgag
      841 cggggccgtc gacgcggcgg tcctgactca tccggaaacg atcatcgcga ggaaacaggg
      901 cttcaacgaa ttgcttttct tcggcgacca tatcgagttt gtctcagcag gggtggtggc
      961 gaccgagaga aacatttctc agaagccgga tttcgtccgc cgtttcatcc gcggcgccct
     1021 caagacgttc tactggatca aggccaacga aaaagaagtt gtgacccggc tggccaaagc
     1081 cacgaagtct tccgagactg acggtctcga actctacaga accggcgtca agctgttttc
     1141 gcaggacggc accatacccc gaagtcttca ggagagaatg atctccatgc agagaaaggt
     1201 ccttcaggta gagaaggaga tcgcgccgga aagtgtctat gacttcagtt ttgtccgtgc
     1261 tgcaaacaaa gagttaggaa aaggagaatc actatgagta ttctcgatca aagtcttctc
     1321 atcgctaatg tgaactacca cgttggtcac tgggtcgcca tttacgtcgc tgaggaacag
     1381 ggttttttca aggaggaagg tcttacgcgc tacgagtacg agcgcggcgg gctcctgccg
     1441 ggcccgtcgg aaagccaaac gcttggactc gcaatcaagg agcacggcgt ggacatcgcc
     1501 acagcggtgg acacagagtc ggccatctgt caaaggcttc aaggcgccga tgtttacatc
     1561 gtcggcggtt ggcgatacac gccgttcctc aaatggtatg gctccaaaca tatcaccgat
     1621 atcagcaaac tccgcggcgg gaagatcggc gtccgggaga gctcggccga cggcggaggc
     1681 ctcgtcgaat tcttcatcga ggaggcattg aaagaggccg gcgtgaaccc ggagaccgag
     1741 gtagaacagg tccaaaaccc ggtgttcggc tatcggaaca acccggccca catcgaaatg
     1801 cttcgctcgg gaaaagtcaa tgcgatcacg tcttcgccgc ccttctccga tcaactcgag
     1861 aaagaaggtt atccggtcat tctcgatccc aacaaagttt ttccccggcg tccgggcaag
     1921 gtcaccgtgg cgccgcgaag aaccatcgaa cagcgcggcg aggagctgaa ggcttacttc
     1981 agggccatca ttcggacatt ttggttcatg cgcgatgtaa agaacttcga atatctccac
     2041 gatcttgaag cgcggctaag aaagctcaca cataacgaag acgagcggag cctctttatc
     2101 gcttcggcgc ccgatcgggt tgacagttgg gcgctgccga tggacggcgg catagagcgt
     2161 caagcgctcg atagaatcat tcaagagatg gtcaagagag gcaggctgga acgtccgctt
     2221 cccgtcgagg aagtattcag agacgatgcg gtgcgggatg cttaccggga cgtcagcaaa
     2281 cgggccaagc tcaagcctgc cttcgataag gccatggcag ccgtagaaaa atatggtttc
     2341 taaacaaaag acgccacggg aatctgaagc cagaaaaaaa ctcgaaactc gaattccgaa
     2401 attcgaacca aaactaactt agaatatctt tggtcccttc accggcgttt ccagcgcagc
     2461 gaggcctttt tcgagaaggc cgacgcgcca ccgcagctcc tcttccgggg gtagctccgg
     2521 ctggcccacg ggatagtgaa acttgctccc tcccagaatc cttcctacgc ccacttgcgt
     2581 tgcgacattg gagaggcaag tgatcacgac cacgggaatt ccttttttct cgatttcttt
     2641 cccgatcg
//
LOCUS       DASSWZ010000095         4737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_512180, whole genome shotgun sequence.
ACCESSION   DASSWZ010000095 DASSWZ010000000
VERSION     DASSWZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512180"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFN58_02400"
     CDS             <1..253
                     /locus_tag="VFN58_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916871.1"
                     /translation="SVKWKNFKVIFRKADGINDPLEDVQVPMVFNLLGDPGERFNLWE
                     RSLDMGWVYRPVFEQIGRFQQSVAKYPNIKTGEDFKGYQ"
     gene            complement(425..574)
                     /locus_tag="VFN58_02405"
     CDS             complement(425..574)
                     /locus_tag="VFN58_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916872.1"
                     /translation="MRLLPNEALQKTRGAGAHGADSLRQAPLAAERQGIKSVFELNSP
                     SKQIL"
     gene            complement(599..1264)
                     /locus_tag="VFN58_02410"
     CDS             complement(599..1264)
                     /locus_tag="VFN58_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4304 domain-containing protein"
                     /protein_id="HET8916873.1"
                     /translation="MSEIGKVIDRIAVDALAAPLKAAGYRRAGRTWRRRLKDAVQVVH
                     VQASRSNVGADGRFTLTAGVYFPALAARLALFPPIDDPAEHDCHVRTRPMPPGRHWWE
                     VRATAAAPDPEAGRVLGAIFSWLDRRADQRAPETNERATQELREALERYALPWLSRVA
                     NLRGARDELARQGLLWWAAAASLDLGVPVEAARLFAQALEKAGPPQADELRRWGRTNG
                     LTP"
     gene            complement(1338..1595)
                     /locus_tag="VFN58_02415"
     CDS             complement(1338..1595)
                     /locus_tag="VFN58_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916874.1"
                     /translation="MKPAEKFFHLIDQFIAMNRTLYERACEMGGTRVTSTAIPFSQAD
                     WIQHYGPIWESFRDAKNRFDPHNVLTLGQGIFPSAVGERRV"
     gene            complement(1675..2505)
                     /locus_tag="VFN58_02420"
     CDS             complement(1675..2505)
                     /locus_tag="VFN58_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET8916875.1"
                     /translation="MSVLCIFCLVACNAAKTISIEALGDTPAGVTTRFVFPTSGSNAE
                     AYLTRPAGPGPFPLMILLHGHNLGSVGATSIVPEAEAFASDLCYAGLAVSLPGFGATE
                     VQPGMDPRITLSVVLGAVSLIKNLPWIDPKQLYLYGFSRGAFYTSLLANQIDGLRGII
                     LHSGAYDLDRLYHETSSQWLRSMLNPSGEENLKLISILPEVSTWSAPTLVLHGEKDFL
                     IPVKQATLLRDSLEVAKKHYRFILYPNSGHRLPPEDVRVKVTAFLKENSGSACRPNAP
                     "
     gene            complement(3083..3994)
                     /locus_tag="VFN58_02425"
     CDS             complement(3083..3994)
                     /locus_tag="VFN58_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916876.1"
                     /translation="MQHTYTYQAALAASERIGWKVDDIIGGDKKLDFSKPFMPESLAQ
                     VKQLSFLTPEEQLTLNQIRGHEYLAMFGLVEEFILPYVVDHARPQLSGDDYRVRALLQ
                     FAGEEAKHIQLFKRFRREFEDGFGSKCDFIGPAEDVSRFVLSHSPLGVGMTILHIEWI
                     TLRHYIEGVRDNQNLDPQFKSLLKHHWMEESQHTKLDTLIVESVVKGATPKEIDQAFE
                     EYCEIGMFLDNGIKQQAEFEAESFVQATGRKLSKNEREEMTNAVLKGMRWTYLGTGMT
                     HPNFLATVEEIKPEARKQIEEMVLAFS"
     gene            4587..4715
                     /locus_tag="VFN58_02430"
     CDS             4587..4715
                     /locus_tag="VFN58_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916877.1"
                     /translation="MMYKMIVAATIAGLLGGYAAMLYALVYVAVLIDRSYLLSLAM"
BASE COUNT         1070 a         1284 c         1326 g         1057 t
ORIGIN      
        1 gtcggtgaag tggaagaact tcaaggtcat tttccgaaag gctgacggta tcaacgaccc
       61 tctcgaggac gtgcaggtgc ccatggtgtt taacctgctc ggtgatcccg gagaacgctt
      121 caatctctgg gagaggtcgc tggacatggg gtgggtgtac cgcccggtgt tcgaacagat
      181 cggaaggttc cagcagagcg tggcgaagta ccccaacatc aaaacaggag aggacttcaa
      241 gggttatcag tagtttgcac ccggcccccg acctgaaaga gacagagcga agacggcacc
      301 cgcaatcgaa tgaaaagagc ggaggacttt caatcctctg cgcgcaaaac taacatccgt
      361 gatttgcatc agtgatggat attattttcc ataggattga actgaacgac gcctcagaca
      421 aatttcacaa gatttgtttc gatggagaat tcagttcaaa aacagactta atgccctgcc
      481 gctcagccgc gagcggcgct tgccgcagcg aatcggcccc atgcgcgcct gcgccgcgcg
      541 tcttctgcag cgcctcgttt ggcagcaacc tcatgaacta gccgccccac cggtcgcctc
      601 acggggtgag cccgttcgtg cggccccagc gacgcagctc gtcggcctgg ggcggccccg
      661 ccttctccaa ggcttgggcg aagagccggg cggcctcgac tggcaccccc agatcgaggc
      721 tcgcggccgc cgcccaccag agcaagcctt gtcgggcgag ctcgtcccgc gcacctcgga
      781 ggttggccac gcgtgacagc cacggcagcg cgtagcgttc cagcgcctcg cgaagctcct
      841 gcgtcgcccg ctcgtttgtc tcgggggccc gctggtcggc ccggcggtcc agccacgaga
      901 agatcgcccc gagtacccgg cccgcctccg ggtccggtgc cgcggcggtg gcgcggacct
      961 cccaccagtg ccggcccggc ggcattggcc gggtgcgcac gtggcagtcg tgctccgcgg
     1021 gatcgtcgat gggtgggaag agcgcgaggc gcgcggcgag cgccgggaag tatacgccag
     1081 cggtaagcgt aaaccggccg tcggcgccaa cgttcgagcg cgacgcctgc acgtgcacca
     1141 cctggacggc atccttgagg cgtctgcgcc aggtgcggcc agccctccgg tagcccgcag
     1201 ccttaagcgg agcggcgagc gcgtctacgg cgatgcggtc gatgactttg ccgatctcgc
     1261 tcattccaaa tcacgccgtg gctagcaaac gttttggttg agaggcgcgg tccaccgcgt
     1321 ccttctcaac ccaatggtta gaccctgcgt tcgccgactg cgctggggaa tattccttgt
     1381 cctagcgtca gcacgttgtg cggatcgaaa cgattcttcg catctcggaa cgattcccaa
     1441 atcggtccat agtgctgaat ccaatcggcc tgcgaaaacg gtatcgctgt cgacgtcacc
     1501 cgcgttccac ccatctcgca tgcccgctcg tatagagtgc gattcatcgc gatgaattga
     1561 tcgatcaagt ggaaaaattt ttcagcaggc ttcaaaaagc tccgggtcta tcctggaagc
     1621 atgtgaattt acgaaggcaa acataaacaa aagggcgagc aacgtctgga ggaatcaagg
     1681 agcatttgga cggcaggccg agccggagtt ttctttgaga aaggcggtga cttttactct
     1741 aacatcttcc ggcggcaggc gatgtccgct attcggatag agaatgaaac ggtagtgttt
     1801 cttggcgact tcgagggagt cgcgtagcag agtcgcctgt ttgacgggaa tcagaaaatc
     1861 tttttctcca tgcaggacca aggtcggtgc ggaccacgtg gagacctccg gcaaaatgct
     1921 aataagtttc aaattttctt cgccgcttgg attcaacatg gagcgtaacc actggctaga
     1981 tgtttcatga tagagtcggt ccaggtcata cgctcctgag tgcaagatta tccctctaag
     2041 gccgtcgatc tggttggcca agagcgaggt gtaaaacgcg ccacgggaga aaccgtaaag
     2101 gtacagttgc tttggatcta tccacgggag attcttaatt agagaaaccg cgcccaagac
     2161 cacgcttaag gtaattcttg gatccatccc cggctgcact tctgttgccc caaagcctgg
     2221 aagcgaaacg gccaaaccgg catagcaaag atcgctcgca aaagcttccg cctctggaac
     2281 gatacttgtg gcacccacgg atccaaggtt gtgaccgtgg agcaagatca taagtgggaa
     2341 cgggccgggc cccgcgggtc tggtaagata ggcctccgca tttgaaccgg aagtcggaaa
     2401 gacaaaacgg gtcgtgacac cggcgggagt gtcgccgagc gcttctatgg aaatcgtctt
     2461 ggcggcgtta caggcaacga ggcagaaaat acaaagaacg gacaagatga atccccgaag
     2521 cagcggccgc gcgcatcgat gaagtatgct tgtggcttca tgaaaggaac cgggctcaca
     2581 ccccgcgaaa actctcatat agtcaagggg tccactagca cactaaggca gttggcatgg
     2641 ttcatttagt cgcgtaagga tatcacactt caaaccagtg cggacaaatt ttttagctaa
     2701 cggtcgagcg aacgggttgg ataccaatta gcggatgacg gagatctgaa tctaagatga
     2761 agcgaaagat gaagtaactc tgatttgcgt atcgacgttc aatagtcacg taaagcctca
     2821 agaatggttg ctggcgcggg cgatgccggt cgtccagagg cggaaagttg gattgatggt
     2881 cgtgccttcc gaatgctggc taacgctttg gctgagagca gtcaaaatag acgcataaag
     2941 tttgctgagg cagttgaatg ttcccgccag cgtagaagtt aaaactccta tttttcgggg
     3001 ctgcgattga cctctgtcat cgatgtatcc gcagcaagcc ggccccaagt tagtagagga
     3061 ccgacgggag gcctgcggcg cgttaggaga acgctagcac catctcctcg atttgctttc
     3121 tcgcttccgg cttgatctcc tcaacggttg cgagaaagtt cggatgcgtc atgccagtcc
     3181 ctaggtacgt ccagcgcatt cctttgagaa cagcgttggt catctcctcg cgctcgttct
     3241 tggagagctt tcgccccgta gcctgtacaa agctttctgc ttcgaactca gcttgctgct
     3301 tgatgccgtt atcgagaaac attccgattt cgcagtactc ctcaaaggcc tgatcgatct
     3361 cttttggggt agcacccttc acgaccgact cgacaatcaa ggtgtcgagt ttggtgtgct
     3421 gcgactcttc catccagtgg tgcttgagta ggctcttgaa ctgcgggtca aggttctggt
     3481 tatccctgac gccctcgatg tagtgcctca gagtgatcca ctcgatgtgg agaatcgtca
     3541 tgccgacacc caacggacta tgtgagagaa caaagcgact cacgtcttcg gcgggaccga
     3601 taaagtcgca tttgctgccg aacccgtcct cgaattctcg ccggaagcgc ttgaaaagct
     3661 ggatgtgctt tgcctcttcg ccggcgaact ggagaagggc ccgcacgcgg tagtcgtctc
     3721 cggagagttg cggccgagca tgatccacta cgtaggggag aataaactcc tcgacgagac
     3781 cgaacatggc gagatactcg tgcccccgaa tctggttcag agtcagctgt tcttcagggg
     3841 tgagaaaaga aagctgcttc acctgggcca aggactccgg catgaacggt ttactgaagt
     3901 cgagcttctt gtcgccacca atgatgtcgt ccactttcca gccaattcgc tcagaggctg
     3961 cgagcgccgc ttggtatgta taagtgtgtt gcatcttcct ttcctccttc ttgaagcacc
     4021 acgatttttc taatcagggc ttactctaga gcaacgaaca tgccacgaat cttaatgagt
     4081 gaaggtggat caagaaccgt ctcaatcttg ctacaggggg agtaacaaat tgattcagtt
     4141 catggcggga cgagctaacg agctttgaag ggcgaatatt ccggataccg cacttctcac
     4201 tcccccttct atcaagattt aggctgagtt accccaacct tcggattaca tccagattca
     4261 agcttgaccc gattgtccta cactttgctc gggatgactg cttgtgatct cacatgagaa
     4321 aacatcgaac ttgaccgccg gtggaaggct cgatacagcc gggcgagaat catgccatac
     4381 ggctaacttt gtctacacta ctgtacccga ggttgacacg gccttgcttt cgaaacctaa
     4441 tcatttttca aattcttgtc tttttccgta agttaaccga ttcgagcgag attcagcact
     4501 tggcatcatg attgcctcgt gtattcagta acacgcttat gcacacaaga catgcttact
     4561 tcggtattaa aaatggaggt gattttatga tgtataagat gattgtcgcc gcgaccattg
     4621 ctgggcttct gggcggatac gctgcgatgt tgtacgcgct cgtctacgtt gcggttctta
     4681 tcgatcgttc ctacttgtta agtctagcga tgtagcgatg agaacctaag ctatctg
//
LOCUS       DASSWZ010000096         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1149056, whole genome shotgun sequence.
ACCESSION   DASSWZ010000096 DASSWZ010000000
VERSION     DASSWZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149056"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFN58_02435"
     CDS             <1..1363
                     /locus_tag="VFN58_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET8916878.1"
                     /translation="FWWGALGSILIFIPYFPRSKHIHLFMAPINLALKKDKPGVLQTM
                     DFENEEIFGAAKLEEFTWPRLLDAYSCIMCNRCQDVCPATNTGKALSPAAILINERYE
                     LNKIFPNFSAGQESPRPLLDFALSEEAAWACTTCNACIEVCPVGNEQMLHIIDVRRER
                     VLAAAEFPNGLKNSFNHMERAGNPWGISADERLVWAKGLPFPVPTIEQNSDPDVLYWV
                     GCAVAFDPRAQKIARSLAEILNSAGLNWAVLGREEKCTGDLARRTGNEYLFAQMATEN
                     VAALDAVKPKVIVTTCPHCFHTIGNEYPQFGGRYVMRHHTEFIDELIRDGRIKLAASA
                     DGSVTYHDPCYLGRHNGVFDQPRALIQSTGARLTEPTRSRSNSFCCGAGGGQFWKDEE
                     KGTERVSTNRYRELKQTGAKTVATGCPFCMRMITEETAKEEPETAMEVLDIAEIVAKN
                     LQK"
     gene            1365..1829
                     /locus_tag="VFN58_02440"
     CDS             1365..1829
                     /locus_tag="VFN58_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET8916879.1"
                     /translation="MRVRRRSLFLTLFAVLLQVSACQAQPQVTIATGGGRELTFQVEV
                     ADTPAKRELGLQYRRDLPPDRGMIFLFPAESEHSFWMKNTPIPLDMIFISKDLKIVGI
                     VEQAVPFSTDSRSVSAASQFVLEINGGLSKRHGIKAGDSVRFQALSPGSVRQ"
     gene            complement(1881..>2507)
                     /locus_tag="VFN58_02445"
     CDS             complement(1881..>2507)
                     /locus_tag="VFN58_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916880.1"
                     /translation="VVSCNTHNLAVLIDTVALGPEKEDNLDEGRFVCMRRANDLSQEG
                     EFIAAPEAGKHDDAEFGTHQGRDAHYLFKTLGLSLNIYSSALKLNTQYMHTIHFNLKL
                     RRGINKDELMRRFQSNGRVALTNKKSSASVFSFGRDHGLFGRILNQTVIVTQSLAMRG
                     DKEIVGTCFTPQDGNSLLSSLAATLWFLYPDSYEQKLEPVRPYFFREI"
BASE COUNT          576 a          671 c          695 g          565 t
ORIGIN      
        1 tttttggtgg ggagccctcg gttcgattct catcttcatc ccatatttcc cccgatccaa
       61 gcatatccat ctcttcatgg ccccgatcaa tctggcgttg aaaaaagaca aacccggagt
      121 cttacagacg atggatttcg agaacgagga gatcttcggc gccgccaaat tggaggaatt
      181 tacctggccc cgattgttag atgcctatag ctgcatcatg tgcaatcgct gccaggatgt
      241 ttgtccggcc acgaataccg ggaaagcgct cagcccagcg gcgattctga tcaatgagcg
      301 ttatgaattg aacaagatct ttcccaattt ctctgcgggg caagagagcc cacgaccgct
      361 gctcgacttt gcgctcagtg aagaagcggc atgggcctgc accacctgca acgcctgcat
      421 cgaggtatgc cccgtcggaa acgagcagat gctccatatt attgatgtgc ggcgcgaacg
      481 ggtgctggcc gcggcggagt ttcccaacgg gctcaaaaat agttttaacc atatggagcg
      541 agcgggtaac ccgtggggca tctcggccga tgagcgactc gtttgggcca agggtttgcc
      601 tttcccggtg ccgacgatcg agcaaaattc cgatccggat gtgctctatt gggttggttg
      661 tgcggtcgcg tttgatcccc gggcgcagaa gatcgcccga agtttggccg agattctgaa
      721 ttccgccggg ctaaactggg cggtcctggg cagagaagaa aagtgcaccg gtgacctggc
      781 gcgtcgcaca ggcaacgagt atctcttcgc ccaaatggcg acagaaaacg tcgcggcttt
      841 ggacgccgtg aagcccaaag ttatcgtcac gacttgtccg cactgctttc acaccattgg
      901 caatgaatac ccccaatttg gcggtcgcta tgtgatgagg caccacacgg aatttatcga
      961 cgagctcatt cgagacggca ggatcaagct cgctgccagc gccgatggtt ccgtgaccta
     1021 tcacgatccc tgttatttgg gccgccataa tggtgtcttc gatcagcccc gcgccctcat
     1081 ccagtccacg ggcgctcggt taactgagcc aacccgctct cggagcaaca gtttctgctg
     1141 tggcgccggt ggtggacaat tttggaagga tgaagagaaa ggcaccgaac gggtcagcac
     1201 aaaccggtat cgtgaattga aacagaccgg cgctaagaca gtggcgacgg gctgtccatt
     1261 ctgcatgcgg atgatcacgg aagagacggc aaaagaagag ccggaaacgg ccatggaagt
     1321 tttggatatt gccgagatcg tcgccaagaa cctgcaaaaa taatatgagg gttcggcgca
     1381 gatctctttt cctgacgttg tttgcggttc tgcttcaggt gagtgcctgc caagcgcaac
     1441 ctcaggtaac tatcgcaaca ggagggggtc gggagctgac ttttcaggtc gaagtcgcgg
     1501 atacgcccgc caagcgagag ctggggctgc aatatcggcg cgacctcccc ccggatcgcg
     1561 ggatgatttt cttatttcca gccgagtccg agcactcctt ctggatgaag aatacgccga
     1621 ttccgctgga catgattttt attagcaagg atctaaaaat tgtcggaatc gtagagcaag
     1681 cggttccgtt ttcaaccgat tcgcgttccg tatctgctgc cagtcaattc gtgctcgaga
     1741 taaatggcgg gctttccaag cgccacggaa tcaaagccgg cgattccgta cggttccaag
     1801 ctctctcgcc aggaagcgtc aggcaataaa gaatcaaaag aaacgctaca gctgacccct
     1861 ttgagggcta gttaaagtga ttagatctca cggaagaagt agggtctaac gggctcgagc
     1921 ttttgctcgt aggaatccgg atacaaaaac cacagggttg ccgcgaggct gctgagcaag
     1981 gagtttccgt cctggggagt aaagcaggtt ccgacaatct ctttatcgcc tctcatcgcc
     2041 aggctctgag tcacgatgac cgtctgattc agaatccgtc caaacaaccc gtggtcgcga
     2101 ccgaaggaaa acaccgaagc ggaggacttc ttgttcgtta aagctactcg tccgttcgac
     2161 tggaaccggc gcatcaactc gtctttgttg atgccgcggc gcagcttgag attaaagtga
     2221 atcgtgtgca tgtattgcgt attcagtttc aatgccgacg agtagatgtt caagctcaat
     2281 cccaacgtct taaagaggta gtgcgcgtcc cgtccttgat gggtgccgaa ttcggcatca
     2341 tcatgtttcc ccgcctccgg cgcagcgata aactcgcctt cctggctcaa atcgttggcc
     2401 cggcgcatgc agacgaaacg cccttcatcg agattatctt ccttctccgg tcctaacgcg
     2461 acggtgtcga tgagcacggc gaggttatgc gtgttacaac tgacgac
//
LOCUS       DASSWZ010000097         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1177528, whole genome shotgun sequence.
ACCESSION   DASSWZ010000097 DASSWZ010000000
VERSION     DASSWZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177528"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VFN58_02450"
     CDS             <1..1035
                     /locus_tag="VFN58_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8916881.1"
                     /translation="DFLDEWFETEVLKATMSASGIIGTFMGPRSPGTAYVLLHHYMGE
                     LDGAFRSWGFARGGMGTISRVIADAARHFGTEIRTSAAVDQVLVRNGRAYGVVLGSGE
                     EIHANIIVSGVDPKRTFLKMVDSQHLSPEFVKQVNNFKIRGSSAKVNLALDALPNFTC
                     LPGNGPHLAGAISISPSIDYIEQAYDDAKYGDFSRRPYLDIIIPSTLDPSMAPPGKHV
                     MSIFVQYAPYHLKHGTWPQRREALGDTVIDTLSEYAPNLKNIILHRQVVTPWDLEQEF
                     GLTEGNIFQGELTLDQLFFLRPVPGWAKYRTPIKNLYMCGACTHPGGGVMAASGRLAA
                     LEILKDWKRI"
     gene            1042..2637
                     /locus_tag="VFN58_02455"
     CDS             1042..2637
                     /locus_tag="VFN58_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET8916882.1"
                     /translation="MQSYDAIIIGAGHNGLVTAAYLGKAGKKALVLERRPAIGGIAAT
                     EEILPGFKYSTCAHLAGTFSQDIIAELELKKHGLEVLPLDPLLFAPLLEGKSFLFPRQ
                     HSEIAEAIGRHSRADATKFELFSVLVKKLAAFLRSVNNLPLSGGTNSGSLNLPELVKL
                     GWKFHRLGEREMYEFLRILPMSVADLLDEWFETDVLKASLAASGILGSFVGPRAQGTS
                     FVFLYHQLGESNGAFRTSGFVRGGIGNLAMAISQTAQRFGAKIQTGVEVTQILTKNGT
                     ATGVVLENGEEISGTAIISSADIKRTFLRLIEPTYLDPEFLLQVKNIRSRGTVAKINF
                     ALDTLPQFKSTQGQSSSANLGGVIHIGPTLDYLEQASDDAKYGRFSRQPFLEITIPSV
                     ADPSLAPSGKHVMSVWMQYAPYHLRDGSWDAKRDALGDTVVSIIEDYAPGFKNSILHR
                     QVLTPLDLEQTFALTEGCIYHAEMSLDQMFFMRPIPGWARYSTPIENLYLCGSGTHPG
                     GGITGLPGYFAAKRILQAWPKTK"
     gene            2734..>3078
                     /locus_tag="VFN58_02460"
     CDS             2734..>3078
                     /locus_tag="VFN58_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916883.1"
                     /translation="MKLYLGLTESSPPNQTRIFLELDDRLVDLNLACAAYLTEVQGEK
                     VNAYELSAYYFPDSIAGLLHRGESALKTLQEVVSFTRKSGTRELHGPAGEKVTYRPTE
                     IRLLPPFQKPEKS"
BASE COUNT          698 a          846 c          830 g          704 t
ORIGIN      
        1 gattttttgg acgaatggtt tgaaaccgag gttctcaaag ctaccatgtc cgctagcggc
       61 atcatcggca ccttcatggg gccgcggtcg cccggcaccg cctatgtcct cctgcatcac
      121 tacatgggag agctggacgg cgcttttcgc tcctggggtt ttgctcgcgg cggaatggga
      181 acgataagcc gggtaattgc cgatgcggcg cgccatttcg gcacggagat tcgcacctcg
      241 gcggctgtcg accaagtgct tgtcaggaac ggccgggcct atggcgtcgt actcggttcg
      301 ggagaagaaa ttcacgcgaa tattatcgtc tccggcgtcg atcctaagcg cacctttcta
      361 aagatggtcg attcgcaaca tctcagtccc gagtttgtca agcaggtgaa caatttcaag
      421 atccggggct cgtcagcaaa agtgaacttg gccctcgatg cgttgccaaa ttttacttgt
      481 cttccaggga acggaccgca cctcgccgga gcaatttcga tcagcccgag catcgactat
      541 attgagcaag cctacgacga tgccaagtac ggcgattttt ctcggcgacc ctatcttgac
      601 atcatcattc cctcgaccct cgatccgtcg atggcgccgc cgggaaagca cgtcatgtcg
      661 atcttcgttc aatacgcacc ttatcacttg aagcacggca cctggccgca gcggcgcgag
      721 gcgctgggcg acaccgtcat cgatacctta agcgaatacg cgcccaattt gaaaaacatc
      781 atcttgcacc gacaggtcgt caccccttgg gatctggaac aagagtttgg tttgaccgag
      841 ggcaatattt ttcagggcga actcaccttg gatcaactct ttttcctccg tcctgtgcca
      901 ggatgggcca agtatcggac accaattaag aatctttaca tgtgtggcgc ctgcacgcac
      961 cccggcggag gggttatggc agcctcgggt cggctggcgg cgttggaaat attgaaagac
     1021 tggaaaagga tttagttcac cgtgcaaagc tacgatgcga ttatcattgg tgccggccat
     1081 aatggactcg tgactgcagc ctaccttggt aaggcaggga aaaaagcttt ggtgctggaa
     1141 cgacggcctg cgatcggcgg catcgccgcc acggaagaga ttcttccggg tttcaagtac
     1201 tcgacctgcg ctcacctggc gggaacattc tcccaggata ttattgcgga gctggagctt
     1261 aaaaagcatg ggctggaggt cctgcctctc gatccgctgc tcttcgctcc gctattagaa
     1321 ggaaaatcgt ttctttttcc ccgccagcat tcagagatcg ccgaggcaat cggccgccat
     1381 tccagagccg acgcgacaaa atttgagctc ttcagcgtac tcgtcaagaa actggctgct
     1441 tttctgcgaa gcgtaaacaa ccttccgcta tctggcggca caaactcggg gagcttgaat
     1501 ctcccagagc tcgtgaagct gggatggaag tttcatcgac ttggcgaaag agaaatgtat
     1561 gaatttctgc gaatcctgcc gatgagcgtt gccgatttgc tcgacgaatg gttcgagacc
     1621 gatgtgctca aggcatctct agccgctagc ggtatccttg gttcgtttgt tggcccgcgc
     1681 gctcagggaa cttcatttgt gtttctctat catcagctcg gggaatcgaa cggcgcgttc
     1741 cggacgtccg gattcgtgcg tggcggcatc ggtaatctcg cgatggcaat ttcacagacc
     1801 gcgcagcgat ttggcgcaaa aatccagacc ggcgtggagg taacgcagat actcaccaag
     1861 aacgggaccg caacgggggt cgtgctggaa aatggcgagg agatttcagg aacggcaatt
     1921 atttcaagcg ccgacattaa gaggaccttt cttcggctga ttgagccgac ctacctcgat
     1981 cccgagttcc tgctacaggt caagaacatt cgttctcgag gcacagttgc caagatcaac
     2041 ttcgcgttgg acaccctccc acaatttaaa tcaacgcaag gtcagtcatc ttccgccaat
     2101 ctgggcggcg tcattcacat cggacccaca ctggattacc tagaacaagc gtcggacgac
     2161 gccaagtacg gacgcttctc tagacaaccg ttcctggaga tcacaattcc ttcggtggcc
     2221 gatccgtctc tcgctccttc cggcaagcat gtcatgtccg tgtggatgca atacgccccc
     2281 tatcatttgc gggatggtag ttgggatgct aagcgggacg ccttgggcga tactgtcgta
     2341 agcatcatcg aggactacgc accgggtttc aaaaattcca ttctgcaccg gcaagtcttg
     2401 acgccgctcg atttggagca gacctttgcc ctaaccgagg ggtgtattta ccacgcggaa
     2461 atgagcttag accaaatgtt tttcatgcgc ccaattccag gttgggctcg atatagcaca
     2521 cccatcgaga atctttacct ttgcggatct ggtacccatc ctggcggcgg aattacgggg
     2581 ctgcccggat attttgcagc gaagagaatc ctgcaggcct ggccaaagac caaatagtct
     2641 ttgcccatgg gttctccgtt tcccactgct agacgcagac atgctggcag tgataagcgt
     2701 aagtgtaagc gtaagcgctc gaggcgacac aagatgaaac tatacctggg cctaacggag
     2761 agctcccctc ctaatcagac acgaattttc ctggagttag atgacagact cgttgattta
     2821 aatctcgcct gtgcagctta tctgacggaa gttcaaggcg agaaggtcaa tgcttacgag
     2881 ctctcggcct actattttcc ggatagcatt gcgggactcc tccaccgggg cgaatccgcc
     2941 ctaaagacgc tccaagaggt cgtctccttc acgcgcaaga gcgggacacg ggagcttcac
     3001 gggccagcgg gcgaaaaagt tacttaccgc ccaacggaaa tccggctatt gccgccgttt
     3061 cagaagccgg aaaagagt
//
LOCUS       DASSWZ010000098         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_172765, whole genome shotgun sequence.
ACCESSION   DASSWZ010000098 DASSWZ010000000
VERSION     DASSWZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172765"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFN58_02465"
     CDS             complement(<1..226)
                     /locus_tag="VFN58_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916884.1"
                     /translation="MSNEDRVIAHSLLRFAAPRAFRFCGRGIIALIAAMLPLSSISTF
                     AQSSPPAKLQKVTALYGARSGASWPMWMAKD"
     gene            complement(223..1467)
                     /locus_tag="VFN58_02470"
     CDS             complement(223..1467)
                     /locus_tag="VFN58_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8916885.1"
                     /translation="MFSREENELLTRVGPGTAMGNLMRRYWIPALLSEEIPLPDCPPV
                     RVRLLSEDLIAFRDSNGRVGLLDEHCSHRGTSLFYGRNEECGLRCIYHGWKYDVEGHV
                     LDTPAEPADSDFKKKLRHTAYPCREVAGIVFTYMGPREKMPLFPAYEWVNLPDDRMHV
                     VKAYLECNYLQGIEGDFDSSHTTFLHCNNLADLARLNRDGAPTLEAEESDYGMRAISI
                     RQLGPDKSYVRISPFIMPAFSIVPGPATVKFEENDSRAFRFWIPIDDTSTWFYILTMR
                     DTPFSAQERASARSWVDSRYFRIRNAGNNYLQDREHQKTRSYSGIKAVIPAEQDGCAT
                     ESMGPIYDRNQEHLGYSDKTIIALRRVLLQAAKSVQEGREPPHLVTDPKLNDFSRLRA
                     VKGVLPANGSWRELPDLEEGMV"
     gene            1778..>2050
                     /locus_tag="VFN58_02475"
     CDS             1778..>2050
                     /locus_tag="VFN58_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916886.1"
                     /translation="MSGQLATRIIDGDGHIMEDNAGIIAHMDPPYREIAQRKGVIFPP
                     LDHLHAGRAVETPPQRDQRPAVGPKGWLDFLDDVGIEWTVLYPTTAL"
BASE COUNT          469 a          583 c          533 g          465 t
ORIGIN      
        1 cgtccttagc catccacatc ggccaggagg cgccgctccg cgcgccatag agagcggtga
       61 ctttctgcaa cttggccggc ggcgagcttt gagcaaatgt cgaaatcgaa ctcagtggca
      121 gcatcgccgc tatgagagcg atgatccctc ggccgcaaaa tcgaaaagct cttggcgccg
      181 cgaatctgag cagactatgc gcaatgactc gatcttcgtt gctcatacca tcccctcttc
      241 caagtccggc agctcgcgcc aggaaccatt cgccggcaaa acccctttaa ccgctctcaa
      301 gcgagagaag tcgttgagtt ttgggtctgt gactagatgc ggaggctccc gtccctcctg
      361 cacgcttttt gccgcttgca gcagaactct gcgcaaggcg atgatcgttt tgtcgctgta
      421 gccaaggtgt tcttggtttc gatcatagat cggccccatg ctttccgtcg cgcagccatc
      481 ctgctcggcg ggaatgactg ccttaatccc cgaatagctg cgggtttttt ggtgttcacg
      541 atcctggaga taattattgc ctgcatttcg aattctgaaa tatcgcgaat cgacccagct
      601 tcgcgcactt gctctctctt gtgcggagaa cggagtatca cgcatggtca agatgtaaaa
      661 ccaagtgctt gtatcatcaa tgggaatcca aaagcgaaag gcccggctgt cattctcttc
      721 aaacttaacg gtggcgggac cgggaacgat gctgaatgcc ggcatgataa aggggctgat
      781 ccgaacgtaa cttttgtccg gccccaattg tcttatagag atcgctctca tgccgtagtc
      841 gctctcctcg gcctctagcg taggtgcacc atcccgattg agccgcgcaa gatccgccaa
      901 gttgttgcaa tgcagaaagg tcgtgtggga tgaatcaaaa tcgccttcta tgccttgaag
      961 gtagttgcac tccagataag ccttgacgac atgcattcga tcatcgggaa ggttcaccca
     1021 ttcgtacgcc ggaaataacg gcattttctc ccgcggcccc atataagtga aaacgatgcc
     1081 cgccacttct ctacaaggat aagccgtatg acgaagcttt tttttgaagt cgctatcggc
     1141 cggctcggcg ggcgtgtcca agacatgccc ttcgacgtcg tatttccagc catgatagat
     1201 gcacctcaaa ccgcactcct cgttgcgccc ataaaagagc gaggtgcccc ggtgagaaca
     1261 gtgttcatcc agaagaccaa ctctgccgtt gctgtcgcga aaggcaatga gatcttcgct
     1321 taaaagccgc acccgcaccg gcggacaatc cggcagcggg atttcctccg acaacaacgc
     1381 tgggatccag taacgccgca tcaaatttcc catagctgtg ccgggaccga ctcgtgttag
     1441 aagttcattc tcctctcggg aaaacatgct cattctcctt tggccgacgg tttcgagtcg
     1501 gacgtgctga agttagatga agccaccgtg gtaggtcaag cggaaactcc ggcaggttgc
     1561 tgaaaaaatt cggaatgctt cgagagcctc agcatgaacg gaaaatcttc aatgatatca
     1621 gaactcctcc gttcgtcctg agcgccgtcg aaggactccg gcagagttgc tcagcagcct
     1681 gttagatctc cggttccttg aagcatgggc tgcatctgga ctataagatc aaggagagcg
     1741 atgagccaat ttctatctaa ttaggaggaa tgtacgaatg tccggtcaac tcgcaaccag
     1801 aatcatcgat ggcgacggtc acatcatgga agacaacgcc gggatcatcg cccacatgga
     1861 tcccccttat cgggagatcg cgcagcgaaa aggcgtcatt ttccccccgc tggatcatct
     1921 ccatgccggg cgcgcagtgg agacgccacc ccagcgcgac cagcggcccg ccgtcggacc
     1981 taaaggctgg cttgattttc tggacgacgt gggaatcgaa tggaccgtgc tctatccgac
     2041 cactgctctt
//
LOCUS       DASSWZ010000099        10867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_158622, whole genome shotgun sequence.
ACCESSION   DASSWZ010000099 DASSWZ010000000
VERSION     DASSWZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158622"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..4017
                     /locus_tag="VFN58_02480"
     CDS             97..4017
                     /locus_tag="VFN58_02480"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET8916887.1"
                     /translation="MSRWFAQNRRIVNKMTRYFQSAAFICVLAVLYFCAGAFGLSLAH
                     VHPSASAVWPPSGIALAAILMWGYRLWPGIFIGSFAVNIAAAGSVVTALSIAAGNSLE
                     AILGAGLVCRFANGPKAFEQTKNILKFILLAGILSTAFGATIGVSSLSLSGLAPWDEF
                     FNIWLTWWLGDAVGNLIVGSLLVLWVTQPLLPIKTLQVLETTGLVVVLVLVGWLLFLT
                     KIPSGLEYFALVPLLWAALRFGRRGAVTSAFAMSAIALWGTLHNLGPFATSNPNQSLL
                     LLQVFLATVTVTSLVMASVILERRRLEQRLRIKDAVSRILAESPSMTDAAPKIIQAMC
                     ETAEWDLGAIWNVDRSSDQLTCIDFWCLPSVNVAAFEALTRQSKFSRGVGLPGRVWSS
                     GKPEWITDVTNDSNFPRAPVATKEGLHTGIGFPIKLADEVIGVMECFSREIEEPDNDF
                     LEMVGNIGAQLGQFMERKRAEDALRESESRLRIALAAGQMGAWEWNLATSRVSWSSSL
                     EVIHGLTPGTFGGSLEDFKRDIHPEDLELVLAQVEKTLVANDDLHVTYRIERPDGVLR
                     WVEGFGHVLLNAAGRPEKLVGVCMDITERKRAEESLRAKEAQVRLITDTAPVMLAQWG
                     RDELYRFVNRTYAERLGLVPEQIVGRSIIEILGKDASAAIRPHIAEVLKGEPVEYESE
                     IPYEKIGRRFMRVAYMPEKDASGNVTGWVTAISDITDRKQTEETLRQQTRALEIVNQE
                     FRKLIDTAPIGIAVATDAEGEHIWCNPELTRMLGTNSGDDVSKSGVAGDKLPFHMLHD
                     GQPVPADDLPMQRACREGIDILDEELEILRSDGTIIHELCRATPLRDGEGKVRGCIGI
                     FLNMTDRKEAEAALQQAKDELARANEELEKRVQERTAELQLANAALSKERDEEKRLEQ
                     QLRQAQKMESIGVLAGGIAHDFNNILNIIKGYASLLRGQGSGNGDLAESLNVIDEAIE
                     RGASTVRQLLAVAKESAVRFEPVDINDALQKLKALLSGTLPRTIDIGLSLDLGLRSVL
                     ADPNQINQVLLNICVNARDAMPEGGELLLQTGTTLGAELRTHFLNAKEKNYACITVKD
                     AGFGMDEATKSRIFEPFFTTKEQGQGTGLGLSVAYGIVTNHGGFIDVTSEPGCGTTFR
                     IYLPLVDGHSVAVEVDGPRGQQEIASIAVHGHVVLFVEDELRQLELMRRSLEKAGYRV
                     LVATDGIEAVEIFLRHKDEISVVVLDLGLPKLNGWEALQKMRKADPTLKPILASGYIS
                     HDMESAMAEGELSALLMKPYEPEEILEKVSLVIRKAANSLNAEG"
     gene            complement(4097..4699)
                     /locus_tag="VFN58_02485"
     CDS             complement(4097..4699)
                     /locus_tag="VFN58_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916888.1"
                     /translation="MRSLSRIGFALALAFLNIQPAFPQVIADTLYRENVEYEDGKISI
                     DFDQTPLGVALSAIQVKTGLQITVPPATESRLLNLRLSRLPLEPAVRSLISSIGFGSF
                     ALMYDEEGLPKRAVVLEARPEVRTAPTADSRGDYSAEPVVEPLRAQERAQLQKELERW
                     GDLKQEERGRIEERLKTLPPSEDREQLVKEYGRQLLGIKN"
     gene            5034..5522
                     /locus_tag="VFN58_02490"
     CDS             5034..5522
                     /locus_tag="VFN58_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916889.1"
                     /translation="MRLAIRAHAGKGSGPNYYFYPLIFAVGLMGCMETINHDASSAGK
                     KAEEFAQVVLVAQDIENGYGLLADGTKRYVSREQFKAVVSKLHPKAFPKTVTAIEYEP
                     MPGEKAIYVFLTGENSGEHFYYRLTMEGTATTGYKVLRLDRASQPYPPSNEKKRFSGQ
                     KG"
     gene            5781..7244
                     /locus_tag="VFN58_02495"
     CDS             5781..7244
                     /locus_tag="VFN58_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET8916890.1"
                     /translation="MKFEELKSLDLRKCNTVGDIVDGMKSCAFGARMLGEVARSIQAM
                     VAAKKKPVLIYSGLENSPLGRLLRKFVENKWCDRMMIPSQYAGQKKQGENAIVVGAFS
                     ERNAEAIYKKPARAIFINPFDMARPGQIRDGYFPDAVFADPRFIMPVLYHSLDEWING
                     KRTSVASLISDLASYGGVASQVAKGADALNDMVRDKQCFRFLTISGAMTIGKMDLVIC
                     DMIESGLTNAISSTGALMAHGLVSSIGLKHYKYNPAYNDTALARRKLNRVTDTLEPET
                     NLDTVEEVIGKVIGQLNGARPISPTLLNKLIGKYLAENYPNERGILKSAYLFGVPVFV
                     PAFVDSELGNDLYIHNIDRKRRGKKSILVDLESDSKELVKLVTAAKRFGIFTIGGGVP
                     RNNVQNVAPLIEIINERLGTHLPPRRFTYGIRICPDRPHFGHLSGCTYSENESWRKAA
                     KNGVYAEIQADATQVWPFLVKYVLDARPVSERNSHHK"
     gene            7412..7795
                     /locus_tag="VFN58_02500"
     CDS             7412..7795
                     /locus_tag="VFN58_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HET8916891.1"
                     /translation="MRKAIRRMITLIILIAGLSVGPVVNLIFAQEIPNQQSKIDPTQL
                     KSFARVYVEFEKIRDAYEPRLSKAQGDQEAQVIQKEAGAKVDEALAKEGLSRETYSQI
                     INTLNADNELRAKAMQLIDEERKKS"
     gene            8066..8905
                     /locus_tag="VFN58_02505"
     CDS             8066..8905
                     /locus_tag="VFN58_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8916892.1"
                     /translation="MNSLHGLAKLLLTVFLFLMPTSVYAQAQPAPAKEQFEPRVGQEG
                     KDVVWVPSPQTVVDKMLDMAKVTPKDFVIDLGSGDGRTVITAAKRGAKALGIEYNPEM
                     VELSKRNAAKEGVSDKATFQKADLFESDFSQATVISMFLLPDINLKLRPKILDLKPGT
                     RIVSNTFTMAEWQADETATVDGECISYCTALLWIVPAKVGGTWQLPQGELALKQTFQM
                     LSGTLKSGNVSTPITNGRLRGDQISFTAGGAQYTGRVNGNAMEGTVKGGSDSKWRATR
                     GGN"
     gene            9066..10058
                     /locus_tag="VFN58_02510"
     CDS             9066..10058
                     /locus_tag="VFN58_02510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916893.1"
                     /translation="MFKRGLTVLLILLSPTAFAVAQEFKSVRMGYPSLGFRQGHIWVA
                     KDEGLFAKYGLDVEPVFLRGGQLAIQALAAGDPPLMSIGQVVQANLSGFNLVLIAGVE
                     SFYDSTIFGRPGTTRLEQLKGKRLGISGYGAATHFAALILLKHLNLEAGKDVILVPGG
                     PDAERVAALSAGKIDASVFNSSTIPVAKKMGFTELVYIPDLGVEVQGNGFATTRAYLK
                     SNRDIVKAALKGYIEGIHFSFHNKPATQKVYAKYMRTNDTDVLEQSYQVYIKTTPKKP
                     YPTLKGLQFLLDQLAPQMPQAKNAKPEQFVDLSLLQELEKEGFFIEMAKRYPSK"
     gene            10109..>10867
                     /locus_tag="VFN58_02515"
     CDS             10109..>10867
                     /locus_tag="VFN58_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916894.1"
                     /translation="MATVDKTLIIDADAHVVESAHTWDYMDSSEKQYRPIPLETREEA
                     GVKLQFWLIDGKVRGFRFPAFSAEELEKRSQQVGRKFADAQESRELGNVDLRLEHMDE
                     TGVDVQVLHNTMFIESATDRAAVEVALCKSWNRWLADIWGQAKGRLRWSCMAPTLSIP
                     DALDQIRYAKENGACAVLMRPVEGSRLLVDPYFYPIYQEASRLDMAIAIHIANGNAWL
                     CDLYRHPVGVASTLHRFRVPTVAAFNDIVLSEIPQ"
BASE COUNT         2592 a         2868 c         2991 g         2416 t
ORIGIN      
        1 acttaacaag aaactgcttt ggctttttct tgctttgttt gacgtccttc ctttttcctt
       61 gtcaacaaca cgcaaatcac ataaaaagaa tctgacatgt cgcgttggtt cgctcagaac
      121 cggcgcattg tcaataaaat gacgcgatat tttcaaagcg cggcattcat ttgcgtcctc
      181 gccgttttat acttttgcgc cggcgctttc ggcttgtcat tggcgcacgt tcatccaagc
      241 gcttctgcgg tctggccgcc cagcggaatt gcgttggcgg ctatccttat gtggggatat
      301 cggctctggc cgggaatatt cattggctcc tttgccgtca acatcgccgc cgcgggatcg
      361 gtcgtcacgg cgcttagcat cgcggcgggc aacagcctcg aagcgatcct gggcgccggc
      421 ttggtttgcc gctttgccaa cggtccgaag gcattcgaac aaacgaagaa cattctcaag
      481 ttcatcctgc tggccgggat tctgagcaca gcgttcggcg ctaccattgg agtatccagc
      541 ctgagcctgt ccggactcgc cccatgggat gaatttttta atatctggct cacctggtgg
      601 ctcggagatg cggtgggcaa cttgatcgtt ggttcgctgc tcgtactttg ggtgacgcaa
      661 ccgttgttgc ccataaaaac cctgcaagtc ttggaaacga caggcttggt ggtcgtattg
      721 gttttggtcg gctggctcct atttttaaca aaaattccct ccggtctgga gtatttcgct
      781 ctagtgcctc tcctttgggc ggcgttgcgt tttggcagac gaggagcggt gacttcagct
      841 tttgcaatgt cggctattgc gctgtggggc acgttgcaca accttggccc gttcgcgact
      901 tccaacccga atcaatctct tttattgctg caggtgttct tggccaccgt taccgtgacc
      961 tctctggtca tggcttcggt tattctggag cgtcgacggc tcgagcaacg actccgtatc
     1021 aaggatgcgg tgagtcgaat cctggctgaa tctcctagta tgacggatgc cgcgcccaaa
     1081 attatccaag ctatgtgtga aacggcggaa tgggacctgg gtgcgatatg gaacgtggac
     1141 cggtcttccg accaactgac ctgcatagac ttttggtgtc ttccttccgt caacgtagcc
     1201 gcgttcgagg cactcacccg ccaaagcaag ttttctcgcg gcgtgggtct gccgggacga
     1261 gtttggagca gcggcaaacc agagtggatt actgacgtca ccaatgacag caattttccg
     1321 cgagcgccgg tggcaaccaa ggagggattg cacaccggca tcggctttcc gatcaaactc
     1381 gccgatgaag tcatcggcgt catggaatgt tttagccgtg agattgagga gccggacaac
     1441 gatttcctgg aaatggtggg aaatatcggt gctcagctag ggcaatttat ggagcgcaag
     1501 cgcgcagaag atgcgctccg ggagagcgaa agccggctgc ggatagccct ggcggcgggg
     1561 cagatggggg cctgggagtg gaaccttgcc acgagccgag tcagctggtc ctccagtctc
     1621 gaagttatcc acggtctgac tccgggaacc tttggcggaa gcctcgagga ttttaagcgc
     1681 gatattcacc ccgaagatct ggaattggtc ttggctcaag tcgaaaaaac tctcgtcgcc
     1741 aacgatgatc tccacgtcac ctatcgcatc gaacgcccgg acggggttct gcgctgggtt
     1801 gaggggttcg gacacgtttt actcaatgcc gccggaaggc ctgagaagct ggtaggggtc
     1861 tgcatggata tcaccgagcg caagcgcgcc gaggaatccc tccgtgccaa ggaagctcag
     1921 gttcgtctca ttaccgacac tgcgcccgtg atgctcgcac aatggggccg cgacgaactc
     1981 tatcgatttg tcaatcgaac ctatgccgag cgcctcggcc tcgtaccgga acaaatcgtg
     2041 ggtagatcga ttattgagat cctgggcaaa gacgccagcg cggcgatccg gccgcatatt
     2101 gcggaggtcc tcaaaggaga accggtcgaa tatgagagcg agattcccta cgaaaaaatc
     2161 ggccgccgct ttatgcgggt cgcttatatg cccgaaaagg atgcgtccgg caacgtcaca
     2221 ggatgggtca cggcgatcag cgacattacc gaccgcaagc agacggaaga gacgttgcgc
     2281 cagcagaccc gcgccttgga aatcgtcaac caggagtttc gcaaattgat cgatacggct
     2341 ccgatcggca ttgcggtggc caccgacgcg gaaggcgaac acatctggtg caacccggaa
     2401 ctcacgcgca tgctggggac aaatagtggc gatgacgtat ccaagagcgg cgtggccggt
     2461 gacaagcttc cgtttcacat gttgcacgat ggtcaaccgg tccctgcgga tgacctgccg
     2521 atgcagcgag cctgtcggga agggattgac attttagacg aggagctgga aatcctgcgt
     2581 agcgacggaa cgattattca tgagctttgc cgtgctacac ctcttcgaga cggcgagggc
     2641 aaggttcgag gctgcatcgg tatttttctg aacatgaccg accgcaagga ggcggaggcg
     2701 gccttgcagc aagcgaagga tgaattggcg cgagcaaacg aggagcttga gaagagagtg
     2761 caggaacgca cggcagaact gcagctggcg aatgccgcgc tgtcgaaaga aagggatgag
     2821 gaaaagagac tggagcaaca gctgcgccaa gctcaaaaga tggaaagcat cggggtactg
     2881 gccggcggca tagctcatga tttcaacaat attctcaata ttatcaaagg atacgcctcg
     2941 cttttgcggg gccagggatc aggcaatggg gatctcgccg aaagtctgaa tgtcatcgac
     3001 gaagcgatcg aacgcggggc ctctaccgtg cgccagctgc tcgccgtcgc caaagagagc
     3061 gccgtccggt tcgaaccagt cgatatcaat gacgcgcttc aaaagctcaa ggcgcttttg
     3121 agcggcaccc taccgagaac gatcgatatt ggtttgagtt tagacctggg tctcaggtcg
     3181 gtgctggctg atccaaacca gatcaatcag gtactgctta atatctgcgt taacgctcgg
     3241 gacgccatgc cggagggcgg ggaattgctt ctgcagaccg ggacgacact cggagccgag
     3301 cttcggaccc attttttgaa cgcgaaagag aaaaattacg cctgcatcac agtgaaggat
     3361 gcgggattcg gaatggacga agctaccaag agccgaatct tcgagccgtt ttttaccacc
     3421 aaggaacaag gccaaggcac cggactcggc ctctctgtcg cctacggaat cgtgacgaac
     3481 cacggcggct tcatcgacgt gaccagcgag cccgggtgtg gtaccacgtt tcgcatctat
     3541 ttgccgctcg tcgacggaca tagcgtcgct gtcgaagtgg atggcccgcg cggccagcag
     3601 gaaattgcaa gcatcgcggt gcatggccat gtagttctgt ttgtcgagga cgagctacgg
     3661 cagctcgagt tgatgcgcag gagtctggaa aaagcgggtt atcgagtctt ggtggccacg
     3721 gacggcatcg aggcggttga gatttttctc cggcacaagg atgaaatttc agtcgtggtc
     3781 ctcgatctcg gactccctaa gttaaacggg tgggaagcac ttcaaaaaat gagaaaagcg
     3841 gacccgacgc tgaaacctat tttggcttcg gggtacattt cacatgatat ggaatccgca
     3901 atggccgaag gggagctgag cgctttactt atgaaaccct atgaacccga agaaatcctc
     3961 gaaaaggtat ccttggttat ccgcaaggct gcaaactccc tgaatgccga gggataaaat
     4021 cttctgatgg taagaaacgt tgagtgattt gattcgccga caaagcccgc cggggtgatc
     4081 gatcggttaa ggccggtcaa tttttgattc ccaagagctg tcggccgtat tctttaacca
     4141 attgctcgcg atcctcagag ggaggaagag ttttcaggcg ttcctcgatt cggccgcgct
     4201 cttcctgctt gaggtcaccc caacgctcca gttccttctg gagctgagct cgttcctgtg
     4261 ccctcagcgg ttcgacgacg ggctccgcac tgtagtcacc cctcgaatca gcagtggggg
     4321 ctgtacgaac ttccggtcgt gcttcgagga caacggcgcg tttgggaaga ccctcttcat
     4381 cgtacattaa agcgaagctg ccgaatccga tggaagaaat aagagaacga acagcaggct
     4441 cgagcggcaa tcggctcagc ctcaagttca gtagtctgct ctctgttgcc ggaggaacgg
     4501 taatctgcaa accggtcttg acttgaattg cgctgagcgc gactccgagc ggagtctgat
     4561 cgaaatcaat ggagatctta ccatcttcat attcgacatt ctcccgatat agcgtatccg
     4621 ctattacttg ggggaaggcc ggttggatat tcagaaaggc tagagcgaga gcaaacccaa
     4681 ttctagaaag gcttcgcatc ccatgtcacc tcttacgaat ccaatacagg caagttctat
     4741 gccctgactt gtcagctaaa tcccgagcga aaaacagctt agccgcttct tgaacggcca
     4801 aaaaccgact acgatcgcac aattttgtcc caaagctgca actgttgcct acgttgattc
     4861 atttgccggt tcctacggtc caccccatcc gcttcctcct tcagaagcgc gtcggcactt
     4921 tgccgactcg ttcatataaa gaaaacttcg ggtggtattt tctgacactt gacgtccggt
     4981 gtcccctgta ctaggtaacc cttcattcca taatgatgtg tagaggttta aacatgaggt
     5041 tggcaatccg ggcacacgca ggcaaaggaa gcgggccgaa ctattatttc tacccattaa
     5101 ttttcgccgt aggtttgatg ggctgcatgg aaacgataaa ccatgatgca tcttcagccg
     5161 gaaagaaagc tgaagaattc gcgcaagtcg tacttgtggc gcaggatatc gaaaacggtt
     5221 acgggttact cgctgatggc acaaaacgct atgtttctcg ggagcaattc aaagccgttg
     5281 tttcaaaatt gcacccgaaa gcctttccga aaactgttac agcgattgaa tatgagccga
     5341 tgcccggcga gaaggcgatt tatgttttcc tgaccggaga gaattcaggg gagcactttt
     5401 actatcgact caccatggaa ggcactgcga cgacgggtta caaagtattg aggctcgatc
     5461 gcgcgagcca accctatcct ccttccaatg aaaaaaaaag atttagcggt caaaaaggat
     5521 aggccgtggc tctcccgcca acttctcgtc ttgagatcaa ccgacggtct gcccgtccac
     5581 tcgaccaccc tttccgatct ttcactcccg ctcgggccgt cggtttatgg ttttcgtttg
     5641 gttacggcgc agtgcaccag ctcgccattt gaaattccat agtcggcaat tgcactggtc
     5701 ggaattgact ggatatactc atcgtgctag gaagcacaaa attattgagg cgagtaggag
     5761 cgaaaagttc cctagagaga atgaaattcg aagaacttaa atcactggat ttacgaaagt
     5821 gcaacaccgt aggcgacatc gtcgacggga tgaaatcctg cgccttcggg gccaggatgc
     5881 tcggcgaagt cgctcgatcg attcaagcaa tggtcgctgc caagaaaaaa ccggtcctca
     5941 tctacagcgg gttggaaaat tctccactgg gacgcctcct gcgtaagttt gtcgagaaca
     6001 aatggtgcga ccggatgatg attccctctc aatatgcagg gcaaaagaag cagggagaaa
     6061 acgccatcgt cgtcggggca ttctccgaaa gaaatgccga agcgatctac aagaaacccg
     6121 cccgcgcgat ctttatcaat ccgttcgata tggcgcgccc tggacaaatc agagacggct
     6181 actttccgga cgccgtgttc gccgatccac gctttataat gccggttctc taccacagcc
     6241 tggatgaatg gatcaatggc aagcgcacct cggtagcctc cctcatatct gatctcgcgt
     6301 cctatggcgg cgtcgcttca caggttgcca aaggagctga tgcgcttaac gacatggtgc
     6361 gcgataaaca atgttttcgt ttccttacga tcagcggcgc catgacgatc ggtaaaatgg
     6421 acctggtcat ctgcgatatg atcgagtcgg ggttgaccaa cgccatttcc tccaccggag
     6481 cgctcatggc ccatggcctc gtctcctcca tcggactgaa gcactacaag tataaccctg
     6541 cctacaacga tacggcgctg gcgcggcgca agctcaaccg ggttacggac acgcttgagc
     6601 ccgagaccaa tctcgatacc gtcgaggaag tcatcggcaa ggtcatcggt caactcaatg
     6661 gcgcgcgccc gatcagcccg acgttgctga acaaacttat cggcaaatat ctcgctgaga
     6721 actatcccaa cgagcggggt atactaaaat ctgcttatct cttcggcgtg cccgtgtttg
     6781 taccggcctt tgtggactcc gagttgggca atgatctcta catccacaac atcgaccgta
     6841 aacgccgcgg caagaaatcg attctcgtgg atcttgaaag tgacagtaaa gagctcgtca
     6901 agttagtgac agcagccaag cgttttggga tttttacaat cggcggcggc gtaccgcgaa
     6961 acaacgtgca aaatgtcgcg ccactgatcg aaatcatcaa tgagcgtctg ggcacgcatc
     7021 tgccgccacg ccgatttacc tacggaatca ggatctgccc cgaccggcct cacttcggcc
     7081 atctttccgg ctgcacctac tctgaaaatg aatcctggcg caaagccgct aaaaacggcg
     7141 tctatgcgga aatccaggcc gacgctactc aggtctggcc gttcctggtg aagtatgtct
     7201 tggacgcgcg cccagtctcg gaaagaaact ctcatcacaa gtgaattttc tctaagcgtg
     7261 ggaatatgag acgaagcgtg caggagaatt catacagaca agcgcgctgc tagttccgtt
     7321 gctcacctaa actctgcaaa tctcacacat aattttttgg catcagtctt gcgccccatg
     7381 tcgttgtttc acgcaattta aggaggtcta aatgcgaaaa gcgatacgtc gaatgattac
     7441 cttgattatt ttgatcgccg gactaagtgt gggaccggtt gtaaacctta tttttgccca
     7501 ggagatacct aaccagcaat ccaaaatcga tccaacccag ctcaaatcat tcgctcgcgt
     7561 ctatgtagaa ttcgaaaaaa tccgtgatgc ctatgagccg cgtcttagca aggctcaagg
     7621 ggatcaggag gcgcaagtca ttcaaaagga agcaggagca aaagtcgatg aagccctcgc
     7681 caaggaaggg ctaagccgcg aaacctacag ccagattatc aacaccttga atgctgacaa
     7741 cgagcttcgc gcgaaggcga tgcagctgat cgatgaggaa cgaaagaagt cctagccgtt
     7801 tcactgcaaa tagtttcaca aacaatttca cagcatttga tagctgatct cattgaagat
     7861 gagattcggc gtgattcgat tgcgcctagt aattgctgtc ggcagctaag cccacacctt
     7921 gagatctatc agcaagcgcg cgactggaaa tttgcttcgc cttagagtag aaagtttaaa
     7981 ctatggcgag gaagatattt cggacgactt gaccgcatgt cgccaaactt ttcctaaagc
     8041 gatctaaacc gtggagagta caaatatgaa ttcattgcat ggtctggcta aattgttact
     8101 gaccgtgttc ttgttcctga tgcctaccag tgtctacgct caggcgcagc ctgctcccgc
     8161 caaggaacaa ttcgaaccac gggtgggtca ggaaggcaag gatgtggtct gggtgccgag
     8221 cccgcagacg gtggtggata aaatgctcga tatggcaaaa gtcacaccca aagatttcgt
     8281 aatcgatctc ggctcgggcg atggtcgcac ggtgattacc gccgcaaagc ggggcgccaa
     8341 ggcgctcggg atcgaataca atccggaaat ggtcgagttg tccaagcgca acgcggccaa
     8401 ggaaggtgtc agcgacaaag ccacatttca gaaggccgac ctattcgaga gcgatttttc
     8461 gcaggccacg gtgatttcga tgtttttact gcctgatata aatctcaagc tgcgtccgaa
     8521 aatcctcgat ctcaagcccg gcacgcgcat tgtgtcgaat accttcacga tggcggaatg
     8581 gcaagctgac gaaaccgcca cggttgacgg agaatgcatc tcctactgta cggccctgct
     8641 ctggatcgtt ccggccaaag taggaggcac atggcaactg ccgcaaggag agctggcgct
     8701 taagcaaacc tttcaaatgc tctcgggtac cttgaagtcg ggcaacgttt ccacacccat
     8761 caccaacggc aggctacgcg gcgatcagat cagtttcact gccggtggcg ctcaatacac
     8821 cggccgcgtg aacggcaatg cgatggaagg aaccgtcaaa ggcggcagcg actccaagtg
     8881 gcgcgcgacc cgcggcggta actagccggg agagtcctcg cgggttcgga tgatccaagt
     8941 ccgaccatct caactccgat tgaagtcgat tcaaaggacc caggtcgagg cgacatgggc
     9001 cttgctttgc gcttgaaccc ggacttctat tacgctttga tagggtttgg tcgtaggagc
     9061 gcattatgtt caaacgcggc ttgactgttc tgctgatcct tctgtcccct accgcttttg
     9121 ccgttgccca agaatttaag agcgtcagga tgggttatcc ctccctgggc ttcaggcagg
     9181 gccacatttg ggttgctaaa gacgaaggat tgttcgcgaa gtatggactg gacgtagaac
     9241 cggttttcct tcgcggcgga caattggcga tccaagcctt ggccgcggga gatccgccac
     9301 tcatgagcat tggccaggtc gtgcaggcca acctgtcggg cttcaacctc gtgctgattg
     9361 ctggagttga aagcttttat gattccacca tcttcggccg tcccggtaca acgcgacttg
     9421 aacaactgaa agggaaaaga ttgggtataa gtggctacgg agctgccaca cactttgcgg
     9481 cgttgattct cctcaaacat ctcaacctcg aggccggtaa ggatgtgatc ctggtgcccg
     9541 gtggcccgga cgcggagcgg gttgcggctt tgtcagccgg taaaattgat gcttcggtct
     9601 tcaactcgtc taccattccg gtggccaaaa aaatgggttt tacggagctg gtgtacattc
     9661 cggatctggg agtggaggtt caggggaacg gcttcgccac cacgcgcgcc tacctcaaaa
     9721 gcaaccgcga tatcgttaag gctgcgctca agggatacat tgagggaatt cacttcagct
     9781 tccataacaa accggcaacc cagaaggtct acgccaaata tatgcgcacc aatgataccg
     9841 acgttctcga acagtcttac caggtgtata taaaaacgac tccgaagaag ccttacccga
     9901 cattgaaagg actccagttt ctcttggacc agcttgcccc gcagatgccg caagcgaaga
     9961 acgccaagcc ggagcagttc gtcgatctga gcctgctgca ggaactggaa aaagaggggt
    10021 tctttattga gatggcgaaa aggtacccat caaaatgagt agaacaaaat ggaactgatc
    10081 cgcgattgcc ggcaggaggg atgagctcat ggcaactgtt gataagacac tgatcatcga
    10141 cgctgacgcg cacgtggtcg agtccgctca cacctgggac tatatggact catccgagaa
    10201 acagtatcgt cccattcccc tggagacccg cgaagaggcc ggagtgaagc tgcaattctg
    10261 gcttatagac ggaaaggtcc gtgggttccg atttcctgct ttctccgctg aagagctgga
    10321 aaaacgatcc caacaggtgg gtcgtaaatt cgccgacgcg caggaatccc gcgagctggg
    10381 taacgtcgat ctgcgcctgg agcacatgga cgagaccggc gttgacgtgc aggtgctgca
    10441 caatacgatg tttatcgagt cagccacgga tcgcgcggcc gtcgaagtcg cgctgtgcaa
    10501 aagttggaac cgctggttgg cggacatctg ggggcaggca aaggggcgtt tgcgctggtc
    10561 ctgtatggca ccgacattga gcataccgga cgcgctggac cagatccgct acgccaaaga
    10621 aaatggcgct tgcgccgtgc tgatgcgccc cgtcgagggc agccgcttgc tcgtagaccc
    10681 gtacttctat ccgatttacc aggaggcaag ccgcctggac atggcaattg ccatacacat
    10741 cgccaacggc aatgcctggc tctgtgattt gtatcgccat cccgtaggag tagcctctac
    10801 gttgcaccgg ttcagagtgc ccaccgtagc cgctttcaac gacatcgtgc tcagtgagat
    10861 tccgcag
//
LOCUS       DASSWZ010000100         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_300394, whole genome shotgun sequence.
ACCESSION   DASSWZ010000100 DASSWZ010000000
VERSION     DASSWZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300394"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFN58_02520"
     CDS             <1..279
                     /locus_tag="VFN58_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET8916895.1"
                     /translation="ATQSQVRRLVVLEAGWMGVLGGILGLFAGTIMSYHHVVYNTKVL
                     TGWTFQFYYPYDVAVLSVVASVILCLIAGYWPSKQAAATPIVAAIGYE"
     gene            complement(387..980)
                     /locus_tag="VFN58_02525"
     CDS             complement(387..980)
                     /locus_tag="VFN58_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8916896.1"
                     /translation="MEKTKHLSLVPWMPALINSLFFCLLFSACSTSNDVQKNTAVPFM
                     PADMQNIGRDNSARSVSAGEALSRGVAAVTPPGAALQDVYYEFDSINLRSDAEDTLKQ
                     NAEWMKANLSARVEIEGHCDDIGSDEYNLALGAKRAQIAKDFLVSQGVSADRLVTISY
                     GKEAPACFEPTEECRVKNRRARFVVFTELSTSYTSVR"
     gene            complement(1077..1568)
                     /locus_tag="VFN58_02530"
     CDS             complement(1077..1568)
                     /locus_tag="VFN58_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET8916897.1"
                     /translation="MRPADRRHHWKTRIKNWLPVCLWASLIFFFSTDHFSFDNTAQFF
                     GFVFSWLWPEMPTEEIAPVHGAMRKVGHWAEYFVLAVLVLRALRNETGKKWELRHAAL
                     ALVFIFVYALSDELHQTFVPSRTASVGDVMIDVLGGICGIFWMYWYSRGILAPLTSRI
                     RES"
     gene            complement(1744..>2067)
                     /locus_tag="VFN58_02535"
     CDS             complement(1744..>2067)
                     /locus_tag="VFN58_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916898.1"
                     /translation="AASLFFLAYGESEGSLWVFTVLFTFMEAIFPVGWATVGDFFGRK
                     SFATIRGTMSFFYLWGPALGPVITGAIYDRYHSYAPMMSGFIALALIASCLYALLVKP
                     ASPSR"
BASE COUNT          501 a          545 c          570 g          451 t
ORIGIN      
        1 gcgactcaaa gtcaagtgcg aaggcttgtc gttttggaag ccggctggat gggcgtgctg
       61 ggaggtattc tgggtttatt cgcggggacc atcatgtcgt atcaccatgt ggtctacaat
      121 accaaagtgc tgactggttg gacgtttcaa ttttactatc cctacgacgt tgcggtgtta
      181 tcggtcgtcg cctccgtcat tctttgcctg atagctgggt actggccgtc caaacaagca
      241 gcggccacgc ccatcgtcgc cgcgattggg tacgagtgaa aaaacctaac tgtcctggat
      301 cagaagttcg atatgaaacg gagtcccaag aaccgtcatt gcgcgcgcac gcaggaatct
      361 agtaggggcg ggtttgaaac ccgccctcag cggacgctgg tataagaggt cgatagctcg
      421 gtaaaaacga caaagcgggc gcggcgattt ttaacgcggc attcctcggt gggctcaaag
      481 caggccggag cttccttgcc gtagctgatc gtgactagtc ggtccgcgga cactccctgg
      541 ctcacaagaa agtctttcgc gatttgggct cgctttgccc ccagagccaa attgtattca
      601 tctgatccga tatcgtcgca atggccttcg atttcaaccc tggcgctaag gttggccttc
      661 atccactccg cattctgttt gagagtatct tcggcgtcac tgcggagatt gatgctgtcg
      721 aattcataat agacgtcttg cagggcggct cctggtgggg tgacggcggc gacgccgcgg
      781 cttagcgctt ctccggccga gaccgagcgc gcggaattat cgcgcccgat attttgcatg
      841 tcggctggca taaacggcac ggccgtgttc ttttggacat cattggacgt ggagcaagcg
      901 gaaaagagga ggcagaaaaa caagctattg atcaacgccg gcatccatgg gacgagcgat
      961 agatgttttg tcttctccat tgcgcgaatt cctgttgagg aaaattctcg ctatggtggc
     1021 gggtcgcgcc gatcttgtca agtttcttta acgatcttgc gggcctgatt cggccttcaa
     1081 ctctctctta ttcgcgaagt cagcggggcc aggatacctc tggaatacca atacatccaa
     1141 aaaattccac agattccgcc aagcacgtcg atcatgacgt cgccgacgct cgccgttcgg
     1201 ctcggaacga aagtctgatg caattcgtcg ctgagggcat agacgaaaat aaacaccaag
     1261 gcgagagcgg cgtggcgcaa ctcccacttt ttaccggttt cattgcgtag cgcccgcagg
     1321 actaaaactg ccaagacgaa gtattcggcc caatgcccca ccttacgcat ggccccatga
     1381 accggcgcga tctcttcggt gggcatctcc ggccacagcc aggagaaaac gaatccaaag
     1441 aattgcgccg tattgtcaaa cgaaaaatga tccgtggaaa agaaaaatat caggcttgcc
     1501 cacaagcaga ccggcagcca attcttgatc cgggttttcc agtgatgccg tcgatcagcg
     1561 gggcgcattg tccgtggcgg cttcaacgta ctggaatgaa cgcgatgaag taaactaaga
     1621 aaaaatccat caccattgtt tattgtagca gattatctgc aaaaaaatcg cacctcggcg
     1681 acctataatt gacaccggag cctaggggcg ggtttgatcc gtgcctggtc gaggaattcc
     1741 ggcctagcgg gaaggagagg caggctttac gagcaaggcg taaagacagc tcgcgatcag
     1801 ggcgagagcg ataaagcctg acatcatcgg cgcatagcta tgatagcggt cgtaaatcgc
     1861 accggtaatg acgggtccca acgccgggcc ccagaggtaa aagaaactca tagtaccccg
     1921 gattgtagca aatgactttc tgccgaagaa gtcgcccacg gtcgcccagc ccaccggaaa
     1981 aatcgcctcc atgaacgtaa agagtacggt gaatacccaa agtgaccctt cgctctctcc
     2041 gtaggcgaga aaaaaaagcg atgctgc
//
LOCUS       DASSWZ010000101         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_88020, whole genome shotgun sequence.
ACCESSION   DASSWZ010000101 DASSWZ010000000
VERSION     DASSWZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88020"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..>2447)
                     /locus_tag="VFN58_02540"
     CDS             complement(435..>2447)
                     /locus_tag="VFN58_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8916899.1"
                     /translation="EERFRSYFELGLIGMAITSPSKGILEVNEEICKTLGYERDELLR
                     KTWTELTHPGDIAADAAEFNRVLVGEIDGYSLDKRFIRKDGRVIYATISLKCLRLADG
                     SVDYFLALVQDITERRQAARRRGVHFAVGQILAESPSLNEATPRILQTVGETLGWEIG
                     AMWIADSDANVLRCLKVWHDPSINVDHFVSVSYGQTIAPGVGLPGRVWTSSRAAWIPD
                     VNNDNNFPCAAFAGEVGLHAGFAFPILFRDKLLGVTEFFSREIRQRDHVLLALFESIG
                     SQIGQFMERKRAEEALREADRRKDEFLAMLGHELRNPLGVISTVVQLLNRKEAPDPEV
                     QDLQNTIKLEVSQLTRLLDDLLDISRIARGLIRLKKEPCDLATIVRHVAEGRRLILEK
                     NGVDLSVQLPSQPVPVMGDSTRLAQIVGNLLDNANKFTDAGGQVTVRLVEEPTGEGAI
                     LTVRDSGIGMAPETLKRVFEPFTQAERTIDRSGGGLGLGLALVKGLVDLHGGEVWASS
                     GGAGCGSEFTIRLSLASETRLLTKPIERGASFTERRRILVIEDNLLAAQSLRMLLTET
                     GHAVEVAHNGPDGIEVARRFRPEIVLCDIGLPGLDGYAVARVLREEPGLTGVYMIAVS
                     GYGQNTDQRRASEEGFNAYLTKPIDFKELDRLLTETATYERPRQLA"
BASE COUNT          541 a          727 c          639 g          540 t
ORIGIN      
        1 cagcagctaa tggaacgagg ctgataaaaa ttcaacctgg tccgagagcc taatcgacaa
       61 tgaagttcgg tgtcaaccgt tgcggaaagg aagcccataa aaagcaaaac acgtcgaacc
      121 catcggcggg atcgaatcga aggacacgag gattgatgat ctcgtagcat ctccaactgc
      181 aagcaaaaca caacgaagtt tctgaatcat ctccgcgtcg caagcttcac acgcactgat
      241 gtgggacaat cggaactaaa ggcgggtgga gaattcgtca agtcatcaaa ccctccggag
      301 ccacctttta aaacaccgag ctttgcggcc caatgactga cgatcggtcc gtcctaccgc
      361 gttcactgta aagcttttct ccggtctgtc cgtgagttgt ccgtctcgtt aaaactgatg
      421 ggctctgggg ttgctcacgc aagctggcgg ggccgctcgt atgtggcggt ctcagtcagg
      481 agtcggtcca gttccttaaa atcgataggc ttggtgagat aggcgttaaa tccttcttcc
      541 gatgcacgcc gctggtcggt gttttgacca tagccgctta ccgcgatcat atatacgccg
      601 gtcagtccgg gttcttcccg caacactcga gcgaccgcat agccgtctaa tcctggcaga
      661 ccgatgtcac acaacacgat ctcgggtcgg aagcgccgtg ccacttcaat tccgtctgga
      721 ccattgtgag ccacctcaac cgcgtgcccg gtttccgtca acaacatccg caggctctgt
      781 gccgccaaca gattgtcttc gataaccaaa atgcgccgcc gctcagtgaa gcttgcgcct
      841 cgctcgatcg gcttggtgag caaccgggtt tcgcttgcca gagaaagtcg gatcgtgaac
      901 tcggacccgc atccagctcc gccgctcgat gcccaaactt caccgccatg tagatcaacc
      961 aaacctttga ccagagccag acctaaaccc aagccacccc cgctgcggtc gatggtgcgc
     1021 tcagcttgag tgaatggctc aaagactcgc tttagcgtct ccggcgccat accgatgccg
     1081 ctatcccgga cagttagaat ggcgccttcc cctgttggtt cttcgactag acgcacagtg
     1141 acttgcccgc cggcatcggt aaatttattg gcattgtcca gtaggtttcc gacgatctgt
     1201 gccagtcgcg tgctgtcgcc catgaccggt accggttgcg atggcaattg aactgaaaga
     1261 tcgacaccat ttttctccag aatgaggcgg cgcccctcag ctacgtggcg cacgatcgtt
     1321 gccaagtcac acggctcttt ttttaatcgg atcagccccc gcgctatacg cgagatatcg
     1381 aggagatcgt cgagcaaccg agtcaactgc gatacttcta gcttgattgt gttctgaagg
     1441 tcctgcacct cgggatcagg ggcttccttt ctattgagca actgtacgac ggtactgatg
     1501 acccctaacg gattgcgcaa ttcgtggccc agcatggcga gaaattcatc cttccggcgg
     1561 tcggcctcgc gcaaagcctc ctcggcgcgt ttgcgctcca tgaactgccc gatctggctt
     1621 ccgatactct cgaacagcgc caggaggaca tgatcccgtt gacggatctc gcggctgaag
     1681 aactccgtca ctccgagcaa cttgtcgcgg aagaggatcg ggaatgcgaa gcctgcgtgg
     1741 agtcctacct caccggcaaa agctgcgcac ggaaaattgt tgtcattgtt aacatcggga
     1801 atccaggcgg cccttgagct tgtccataca cggcccggca aacctactcc cggcgcgatc
     1861 gtctgcccgt agctcaccga tacgaaatgg tccacgttga tggatggatc atgccagacc
     1921 ttgaggcacc gcagcacatt cgcgtccgag tccgctatcc acatagcacc gatttcccag
     1981 cccaacgttt caccaaccgt ctggagaatc ctcggcgtcg cttcgttcag tgacggagac
     2041 tcagcgagga tttgaccgac ggcaaaatgg acaccgcgac gtcgcgctgc ctgtctgcgc
     2101 tcggtgatgt cttgtactag agccaagaag taatccaccg aaccgtccgc gagacgcagg
     2161 catttgagcg aaatggtagc gtagataacc cggccgtcct tgcggataaa tcgtttgtct
     2221 agggaataac cgtcgatctc cccgacgaga acacggttaa attccgcggc atcggcggcg
     2281 atatcgccgg gatgagtcag ctccgtccac gttttgcgca acagttcgtc gcgttcgtag
     2341 cccagcgttt tgcaaatctc ttcattgacc tcgagtatgc ctttgctcgg cgatgtgatc
     2401 gccatgccga tcaagccgag ttcgaagtag ctccggaatc gttcttc
//
LOCUS       DASSWZ010000102         6204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_385163, whole genome shotgun sequence.
ACCESSION   DASSWZ010000102 DASSWZ010000000
VERSION     DASSWZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385163"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFN58_02545"
     CDS             complement(<1..239)
                     /locus_tag="VFN58_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916900.1"
                     /translation="MSAKTSYVIKLSEDLYLDSNNNFLTSALPNLPVYALAGGDLPLS
                     DNTRKALLHQILAVLRKDDPRWKTILRSIGIGAELT"
     gene            complement(464..691)
                     /locus_tag="VFN58_02550"
     CDS             complement(464..691)
                     /locus_tag="VFN58_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916901.1"
                     /translation="MAAIPPIKCYCTIAQDKIVFCPLHREAETMLAMCEYLRKFLASV
                     KVADPSDENLIREVILPCVDGAIAAATAKKS"
     gene            958..2796
                     /gene="ftsH"
                     /locus_tag="VFN58_02555"
     CDS             958..2796
                     /gene="ftsH"
                     /locus_tag="VFN58_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET8916902.1"
                     /translation="MSSSSKHIALWLVLALVFVVIFSVFNKQYRREPEIIFSEFMGGI
                     ERGEIREVVIQGNHIQGKYKNGEQFRTFAPDDPELVKSLRDKGVKIAAKPADESPWYW
                     VLLLSWLPMLLLIGVWIFFMRQMQVGGGKAMSFGKSRAKLLTENQHRVTFSDVAGVDE
                     AKDDLQEIIAFLKDPKKFTKLGGRIPKGCLLVGAPGTGKTLLARAIAGEAGVPFFSIS
                     GSDFVEMFVGVGASRVRDLFVQGKKKAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRHVVVPRPDIRGREGILQV
                     HTRKVPLSTDVDVSVLARSTPGFTGADLENLVNEAALLAARNDKEKVDMLDLELAKDK
                     VMMGAERRSMIISDEEKRNTAYHEAGHALVAKLLPGADPIHKVTIIPRGMALGLTQQL
                     PIDEKHTYPREYLLNNLVILFGGRVAEELVLEHTTTGAGNDIEKATDLARRMVCEWGM
                     SEKLGPMTFGKKEEEIFLGRDFTQKVDYSKSTAVEIDAEIRRIIQESYHRAKDLLKTN
                     LGLLHKVAENLLEKEVLDGSEIDAIVRAFGGNGGEAFAPSSAAAIA"
     gene            3026..4135
                     /locus_tag="VFN58_02560"
     CDS             3026..4135
                     /locus_tag="VFN58_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916903.1"
                     /translation="MAIGLSHGGNNVYSSNEPSRQLWVGTKDGLVLFERADDGRWREA
                     HRALRGEHISSIIFERTSGLMFAGAFFGSVHASPDGGKTWERRDNGIPIHDVYSLASN
                     VVDGKVRVYAGTQPAHLFCSEDLGQHWTELPALRSVPSTPNWSFPAPPHIAHTKFITF
                     DPRDSSTIYACIEQGALLKSSDSGKSWAELNTVGFFRDKDRPAEHFYDVHKALIDPRD
                     PKKIFVTGGAGFYVSADGGSRWERWTSPDWAEDVYPDGFVLNPRNPDVMFVAAAEHNP
                     TTWRKSAYAGGRIYRSDNGGKNWQRLGGGLPESLKHEFGALCLEDWGDGYSVFGATTG
                     GEVYCGDANGERWILIASDLAPISKKGHERLLAAS"
     gene            4257..4400
                     /locus_tag="VFN58_02565"
     CDS             4257..4400
                     /locus_tag="VFN58_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916904.1"
                     /translation="MTSPIANPGTALAEELCRHSEQDAAVTPMDPVADARAREQALGR
                     SVF"
     gene            4450..4725
                     /locus_tag="VFN58_02570"
     CDS             4450..4725
                     /locus_tag="VFN58_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916905.1"
                     /translation="MTVQQQFRRRLFFLGFIWLTVIAASAHAQSSSKKVRIGVSETHV
                     SYLPAEEVQFLIDLMRTNAKISRPVTVEQVADFSFVDKARKDLGLTR"
     gene            4822..5454
                     /locus_tag="VFN58_02575"
     CDS             4822..5454
                     /locus_tag="VFN58_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET8916906.1"
                     /translation="MIDFEGRKIPNELNEIVEASRTVLLVWDMQNDQAGGAFNKDALI
                     RNVPPLISAAAGAGIKTVYTRQTPYLWKDESPAWIHRFMQTQKIDHPSKLTPRRLHGS
                     FGWQIMEPFKPGENDIVIDKRRPTMFIGNEFETVISNRRAHTIVMIGCTTDGGVEATV
                     RDGYHRGYFMVVVRDCVGTYTEEGHNAALKRMDRFADVVNSQDLIQIWRH"
     gene            5528..>6204
                     /locus_tag="VFN58_02580"
     CDS             5528..>6204
                     /locus_tag="VFN58_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916907.1"
                     /translation="MSGANRKNNALHVATMSGWYRFEKDGQGWKQVKRDLSYWTLTCL
                     AVDPENPELIYAGTEHSGLFYSKDAGAHWLRANPNVPKMMLFSALALNGGVMVGTVPS
                     AVFRSKSGGGWEELEGVRINSANANFPPSPELQSRARYLASDPLMPARLYAGIEVGGM
                     LLSDDGGRNWQPANNGLTDMDVHEVHPCEKSPGVVYAACGDKAFRSFDRAAHWEEITP
                     KSHDYGMS"
BASE COUNT         1432 a         1595 c         1801 g         1376 t
ORIGIN      
        1 gtcagttccg ctccaattcc aatcgagcgc agaatggtct tccaccgcgg gtcgtctttg
       61 cgcagcacgg caagtatttg gtgcagcaag gccttgcggg tgttgtcgct cagcggaagg
      121 tcgccgcccg caagggcgta aaccggcaag tttggcaggg cgcttgtaag gaaattatta
      181 ttgctgtcca ggtagagatc ttcgctcagt ttaatcacgt acgacgtctt tgcggacatg
      241 cgagcctcct cgtgaaccag agtttagtgg atcatatgct gccggcgaga gattgctgtc
      301 caatattaga cagtttttaa ggataattgg ggaagcgagg caaagatttg gagttatgta
      361 gttgcgccga gataaatccg cctcatatca gtaccggaaa cctttcgcgg tttccttttg
      421 gttcgcgcaa gctcgaccca ataagtatcg tgatatgggg ttgctaactc tttttcgccg
      481 ttgccgcggc aatcgcaccg tccacgcaag gcaatatgac ttctctaatc agattttcat
      541 cacttggatc ggccaccttg acgcttgcga ggaactttcg caggtactcg cacatcgcca
      601 gcatcgtttc tgcctcccgg tggagaggac aaaaaacgat tttgtcttgg gctatcgtgc
      661 agtaacactt gatcggtggt attgcggcca cggttatttc cagaactgcc ccgaaggcca
      721 gaaattggcg ttcttttttt ggccctgatt gcggcgggat ccttgcctga tactgaaatt
      781 atttttagtt tctgtcaagc aacggccgat cttatcatcc tctgagcttt atgagttacg
      841 aaagggctat cctgcttgga ggcgacaaaa aagtgccttc cgttgcgtgt ctgtactcca
      901 agagctagaa tcaatatctt cgatgtgaaa ggggtttttc ctggacggag ggatttcttg
      961 agttcttcat caaaacatat tgcactttgg ttggtcttgg cgctcgtttt tgttgtgatc
     1021 ttcagcgttt ttaacaaaca ataccggcgc gaaccggaaa ttattttcag cgaatttatg
     1081 ggtggcatcg agcgcggcga gattcgagag gtcgtgatcc aaggaaatca tattcagggc
     1141 aaatacaaaa acggcgagca gttcagaact ttcgcgccgg atgatccgga gctggtgaag
     1201 tcattgcggg ataagggcgt gaaaatcgct gccaagcccg cggacgagtc gccgtggtac
     1261 tgggtgctac tgcttagctg gcttcccatg ctgcttctga tcggggtttg gatctttttt
     1321 atgcgccaga tgcaggtggg cggcggcaag gcgatgtcct ttggcaagag ccgcgcaaaa
     1381 ctgcttacag agaatcaaca ccgcgtaacc ttcagcgatg tcgccggcgt ggatgaggcc
     1441 aaagatgatc tgcaagagat tatcgcattc ctaaaagatc caaaaaaatt cacgaaactt
     1501 ggcggcagaa ttcccaaagg gtgtttgctc gtgggggccc cgggaactgg caagaccctg
     1561 ttggctcgtg ccatcgcggg cgaagccggg gtcccgttct tcagtatcag cggttccgat
     1621 ttcgtagaaa tgttcgtcgg cgttggagct tcccgagtcc gtgacctttt cgttcaaggc
     1681 aaaaagaagg cgccttgcat catttttatc gacgaaatcg acgcggttgg gcgtcaccgc
     1741 ggagccggcc tcggtggcgg ccatgacgag cgggaacaga cgttgaacca gctgttggtc
     1801 gagatggatg gattcgagtc caacgaggga gtcattttga tcgctgccac caaccggcct
     1861 gacgtattgg atcccgcgtt gctccgtcct ggccggttcg accggcacgt agtagtgcca
     1921 cgaccggaca tcagagggcg agaaggtatc ttgcaggtgc acacccggaa ggtgccgttg
     1981 tcaacagacg tggacgtcag cgtgctggcg cgatccacgc ccgggtttac cggcgcggac
     2041 ttggagaatc tcgtaaacga agcggctttg ttggcagctc ggaatgacaa ggaaaaagtc
     2101 gacatgctcg atttggagct ggccaaggac aaggtcatga tgggtgcgga gcggcgcagc
     2161 atgatcatta gcgacgagga gaagcgcaac acggcttatc acgaagccgg gcatgcattg
     2221 gtagcgaagt tactccctgg cgccgacccg attcacaaag tgacgatcat tcctcgcggc
     2281 atggctttgg gtctgacgca gcaactgccc atagacgaaa agcataccta cccccgggaa
     2341 tatctcctca ataatttggt cattctgttc ggcgggcgcg tggcggaaga gcttgtgctc
     2401 gagcatacga cgacgggcgc ggggaatgat atcgaaaagg cgacggatct ggcgcgccgg
     2461 atggtctgcg agtggggaat gagcgaaaag ctagggccaa tgactttcgg caagaaagaa
     2521 gaagagattt ttctgggcag agactttacg cagaaagtag actattccaa gagcaccgcc
     2581 gttgaaatag acgctgagat caggcgcatt atccaggaga gctaccatcg ggctaaggac
     2641 ctgttaaaga ctaatctggg tctgctgcat aaagttgcgg aaaacttgct cgaaaaagag
     2701 gttctagacg gttcggagat tgacgccata gtcagagctt tcggcggtaa cggtggagaa
     2761 gcgttcgcac catcgagcgc ggcggccatt gcttaagtag acccgtatcc cctgaattca
     2821 aagctaacga aacagcctcg gtgcccgacc ggcctagact ggtcctgtta ccgactcgga
     2881 caccaccccc tacgattcgg aagtgcccgc gtaaatgaga cttggccaac cattgacggg
     2941 caagaacatg ggttggtaga gtgtgccacg tgtgatatct agggcttatt gagcaataac
     3001 tatcgcaacc ctaaggagga aagctatggc tatcggactt tctcatggtg gcaataacgt
     3061 ttattcatcc aacgagccgt cgagacaact atgggttggg accaaggatg gcttggtcct
     3121 gttcgagcgc gcggacgatg gcagatggcg cgaggcccat cgcgcgttgc gcggcgagca
     3181 tatcagttcc attatttttg agcggaccag cggcctgatg tttgccggcg cgttctttgg
     3241 ttcggttcat gcgagcccgg acggcggcaa aacctgggag cggcgcgaca atggtatccc
     3301 gattcatgac gtttacagct tggcttcaaa cgtcgtggac ggcaaagttc gggtttatgc
     3361 cggcacgcaa ccagcgcacc tgttctgcag cgaagaccta ggccaacact ggactgagct
     3421 gccggcgctg cgctcagtac ccagtactcc aaactggtcg tttccagctc ccccgcacat
     3481 cgcgcacacc aagttcatca cttttgatcc ccgcgattcc agcaccattt acgcctgcat
     3541 cgagcaaggc gcgttgttga aaagcagcga ctcgggaaaa agttgggcgg agctaaatac
     3601 cgtcggcttt ttccgcgaca aagatcgccc cgccgagcat ttttatgacg ttcacaaagc
     3661 tctcatcgac ccacgcgatc ctaaaaaaat ctttgtgacg ggtggagccg ggttttatgt
     3721 cagcgcggat ggcggcagcc gctgggagcg ctggaccagc cctgattggg ctgaggatgt
     3781 ctatccggac ggtttcgttt tgaacccacg aaatccggat gtcatgttcg tcgcggccgc
     3841 tgaacacaac cccaccacct ggcgaaagag tgcctacgcc ggtggccgga tttaccgcag
     3901 cgacaacggc ggcaagaact ggcaacggct aggcggaggg ctcccggaga gtttgaagca
     3961 tgagttcggt gccttgtgcc tggaagactg gggcgatggc tattcggtct tcggtgcgac
     4021 cactggcggg gaggtttatt gcggtgacgc caacggcgaa cgctggatct tgattgcgtc
     4081 ggacctggcg ccgatttcca agaaggggca cgagcggctg ctcgcagcga gctaaagacc
     4141 caagcggaca aatcgctgtc tccacgacac cgatttcaat cgtactttct cttccttgac
     4201 cggggagaat tgaacagagg gggtgtttag tttaacggcc gaggttcgtc gcgtctatga
     4261 cgtctccgat tgcaaatccc ggaaccgctc tggcggagga actgtgtcgc cacagcgaac
     4321 aggacgccgc ggtcacgccc atggacccgg tggcggatgc ccgcgcgcga gagcaagccc
     4381 tcggacgatc agtgttttag gcttctcggt cgtctattga ataataggtt ccacagcgtc
     4441 aggtcctaga tgaccgtcca acaacagttc agacgaagat tattttttct cggcttcatc
     4501 tggttaactg tcattgcagc cagcgcacat gcgcaatcca gctcgaagaa ggttcgcatt
     4561 ggggtttccg aaacccatgt cagctacttg cccgctgaag aagttcagtt tttgatcgat
     4621 cttatgcgca ccaacgccaa aatttcccga ccggttacgg tggagcaggt cgctgacttc
     4681 agttttgtcg ataaggcgcg aaaagatttg ggattgaccc gctgacaagt cctggctgag
     4741 ctgaaaatct caaattgcaa atggcagatc tcagatttcg aattgaaaca tgctgtttga
     4801 aattcttaag tggagatcga tatgattgat ttcgaaggga gaaagatacc caacgagttg
     4861 aatgagatcg tcgaggcgag ccgtacggtt cttttggtgt gggacatgca gaacgatcag
     4921 gcgggcggcg cattcaacaa ggatgcgctg atacgaaatg tcccaccgct catttctgcg
     4981 gcggcaggcg ctgggatcaa aaccgtctat acgcgccaga ctccctactt gtggaaagac
     5041 gaatcaccgg cttggattca tcgcttcatg caaacacaaa aaatcgatca ccccagcaag
     5101 ctcaccccgc gccggcttca cggcagcttc ggctggcaga tcatggaacc ttttaagccc
     5161 ggcgagaacg acatcgtcat cgacaagcgg cgcccgacca tgttcatcgg caatgaattc
     5221 gagaccgtga tctcgaatcg gagagcccat acgatcgtga tgatcggttg caccaccgac
     5281 ggcggggtcg aagccacggt gagggacggc tatcatcgcg gctactttat ggtcgttgtc
     5341 cgcgattgcg tcggcactta caccgaagag ggccacaatg cagcgctcaa gcgcatggat
     5401 cggttcgccg atgttgtcaa ttctcaagac ctcattcaga tctggcgaca ctgacggaag
     5461 acggaaattc acgatttatg ccaggaacga ttaatggtat ccgaaaccaa catgagagga
     5521 gaaaaagatg agcggcgcga ataggaaaaa taatgcctta catgtggcca cgatgtcagg
     5581 ttggtatcgc tttgaaaagg acgggcaagg atggaaacaa gtcaagcgcg acttgagtta
     5641 ctggacgctg acctgcctgg ccgtagaccc ggaaaatccc gagttgatct acgcaggcac
     5701 cgagcactcc ggtctttttt acagcaaaga cgcgggtgcg cactggctaa gagccaaccc
     5761 caacgttccg aagatgatgt tgttttcggc gctggcgctg aacggcggag tcatggtcgg
     5821 tacggttccg tcggcggttt ttcgcagcaa atccggcggc ggatgggaag agttggaggg
     5881 cgtgcgcatc aacagcgcta acgcgaactt cccgcccagc cccgagctgc aatcgcgtgc
     5941 ccgctacttg gcctccgacc cgctgatgcc ggcgcggctc tacgccggca tcgaagtcgg
     6001 cggtatgctc ctcagtgacg atggcggtcg taactggcag ccggccaaca atgggcttac
     6061 agacatggac gtgcacgaag ttcacccctg cgagaaatcg cccggagtgg tctatgcggc
     6121 ttgcggcgac aaagcttttc gcagcttcga ccgcgccgct cattgggaag aaatcacacc
     6181 caagagccac gactacggca tgtc
//
LOCUS       DASSWZ010000103         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_724809, whole genome shotgun sequence.
ACCESSION   DASSWZ010000103 DASSWZ010000000
VERSION     DASSWZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724809"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFN58_02585"
     CDS             complement(<1..654)
                     /locus_tag="VFN58_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8916908.1"
                     /translation="MNVVFWKELADHFSSRRFMILLALIVLIGLWATYASGQAIRQDT
                     ENAPTQYVFLFLLTSGTGTVFSLATFLGFFGPLVGITLGFDSISGEYARGTLSRVLSQ
                     PIYRDSLINGKFFAGLATVAILWGSILLLVIGLGITLLGFPPNAEELWRMLIFTAVGI
                     FYVGFWLALAMLFSLLFQKTVTAALASMAVWLFMTLFVVIISQGVAGIVVPDPSTPEQ
                     "
     gene            complement(656..1588)
                     /locus_tag="VFN58_02590"
     CDS             complement(656..1588)
                     /locus_tag="VFN58_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916909.1"
                     /translation="MSAVVETQNLTKRYHDKLAVNALNLTVQEGEIFGFLGPNGAGKT
                     TTILMLLGLTEATSGRASVCGFDPTHESLEVKRRVGYLPENPGFYEDMSAAENLLYMA
                     RLNRIPDDEARRRTSEVLDQVGLSEDGRRLVREFSRGMKQRLGIAEVLVKKPKAVILD
                     EPTLGIDPDGAIRILELIKDLNRERNLTVMLSSHQLQQVQEICSRVGIIVKGRLIVQG
                     QMDELGRAILKERQWNFLLEVGAGANGLENDLRAINGVDEIEKRGHGLFLRCTRDVRP
                     EVISLLARKNLPLLQLRSEDPTLEEIYLKYFREA"
     gene            complement(1585..>2011)
                     /locus_tag="VFN58_02595"
     CDS             complement(1585..>2011)
                     /locus_tag="VFN58_02595"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HET8916910.1"
                     /translation="LKGTQDLKMGSAAGTLNTSLTAGKKTPVDFLVGNAGTAPIRNLS
                     FLSKKPNDKWTVEFRPDKIDALNSGEVKEIKMEILAPDRTIAGDYLLTLTANAPEVNK
                     SVEFRVTVSTPTVWGWVGFGIVALVVLGLAVVFFRLGRR"
BASE COUNT          473 a          543 c          559 g          436 t
ORIGIN      
        1 ctgttccggc gtcgacggat cggggactac aattccggca acgccttgac tgatgatgac
       61 gacgaagagc gtcatgaaga gccacaccgc catcgacgca agcgccgcgg tcacagtctt
      121 ctgaaagagc aaagagaaca gcatagcgag agcaagccag aaaccgacgt agaagatgcc
      181 taccgcggtg aaaataagca tacgccagag ttcttcagcg ttgggaggaa atccaagcaa
      241 tgtgatccct aagccgatca cgagcaagag aatcgagccc cagaggatgg ctacggttgc
      301 caagccggca aaaaatttgc cattgatcag gctgtcgcga tagatgggtt gcgaaagcac
      361 gcgactcaaa gttccccgcg catattcgcc gctgatggaa tcaaaaccca aggttatgcc
      421 gacgaggggg ccgaagaagc ccaaaaaagt cgccagagag aacaccgtac cagtgcccga
      481 tgtcagcaag aacagaaaga catattgtgt cggagcattc tcggtgtctt ggcggatggc
      541 ttggcccgag gcataggtgg cccacaggcc gatcaaaaca atgagcgcga gaagtatcat
      601 gaagcggcgg ctgctgaagt gatcggctaa ttctttccag aaaacgacgt tcattttatg
      661 cctcgcggaa gtatttcaga tagatttcct ctagagtcgg gtcttcagaa cgaagctgca
      721 agagaggcag gttcttccgt gccagcaatg atatgacctc gggccgcaca tctctggtgc
      781 aacggagaaa taagccgtga ccgcgctttt caatttcatc gactccgttg attgcacgca
      841 ggtcattttc gaggccgttg gcgcctgcgc ccacctccaa tagaaagttc cactgccgtt
      901 ctttgagaat ggctcttccc aactcgtcca tctgtccctg gacaatgagt cgtcccttga
      961 caatgatccc gacgcggctg caaatctctt gtacttgctg aagctggtgg gaggagagca
     1021 tcactgtcag gtttcgttcc cgattgagat ccttgatcag ctccaggatt cgaatcgccc
     1081 cgtccggatc tatgcccagg gtcggctcat cgaggatgac ggccttgggt tttttcacca
     1141 agacttcggc gatgcctaag cgctgtttca taccgcggga gaattcccgc accaagcggc
     1201 gaccgtcttc gctcaggccg acctggtcta agacctctga agtccgtcgc cgagcctcgt
     1261 catctgggat acggttcagc cgcgccatgt agagcaggtt ttctgcggcg ctcatgtcct
     1321 catagaaacc ggggttttct ggcaggtagc cgacacggcg ctttacttcg agagattcgt
     1381 gcgtcggatc gaagccacaa acagaagcgc gtcccgatgt ggcctccgtg agtcccagga
     1441 gcatcaggat cgtggttgtc ttcccggcgc cattgggtcc aagaaaaccg aatatctcgc
     1501 cctcttggac agtcagatta agcgcgttga cggcgagctt atcgtgatag cgcttcgtaa
     1561 ggttttgggt ctcgacaaca gcgctcatcg gcgtcctagg cggaaaaata ccaccgccag
     1621 tcccaaaacc accaaagcaa cgatgccgaa tccgacccag ccccatacgg taggcgtcga
     1681 aactgtgacc ctgaactcca cagacttatt gacttcgggg gcattggcgg tcaaggtcaa
     1741 aagataatcc ccggcgatag ttcgatccgg tgcgagaatt tccatcttga tctctttcac
     1801 ctcgccggag ttgagtgcgt cgattttgtc cggcctgaac tcgaccgtcc atttatcatt
     1861 aggctttttg ctgagaaagg ataggttgcg gatgggtgcg gtgccggcgt tgccgacgag
     1921 aaaatctacc ggcgttttct tgccggcggt cagggatgta ttaagagtgc cggcagccga
     1981 gcccatcttg aggtcttggg tgcctttgag a
//
LOCUS       DASSWZ010000104         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_923001, whole genome shotgun sequence.
ACCESSION   DASSWZ010000104 DASSWZ010000000
VERSION     DASSWZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923001"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VFN58_02600"
     CDS             <1..172
                     /locus_tag="VFN58_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HET8916911.1"
                     /translation="VIFVHNHPSGDPAPSQEDIEITRRLKEVGEVMGIRVLDHVVIGL
                     ERYYSFSDRGML"
     gene            330..1745
                     /locus_tag="VFN58_02605"
     CDS             330..1745
                     /locus_tag="VFN58_02605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET8916912.1"
                     /translation="MTALELGRPPRRTHVLRNIIIVTLLAAIIAGGYFLVPKFEWYKP
                     QVKITPDTDTLGLAPIEIEINEAGTGLKSVAVTLSTGGTERPLFSQEYDPPVMQAKVT
                     VASSKLSGIKEGPAIFRVSARDRSLWSFFRGNETVIQKNVTIDITPPALELIADDPYV
                     NFGGVGLVAYKTSSDAVTSGVKIGSYFFPGYKGQTKDPDTYISFFAHPYNVSEGEKAV
                     LVATDKAGNSRELRLAYTLKDVKYKKSTIPVSDDFIQGKVAPLLADVGARQGSPKDIF
                     VKVNHGLRKENEDKIRMVGQKTANRMLWSGPFIQLSNSKVEANFADSRTYIYRDEPID
                     TAYHLGFDLSVTKRYPVEAANSGVVSFVGDLGIYGNTVIIDHGLGLSTLYGHLSSVDV
                     KEGEQIKQQQIIGKTGETGLAVGDHLHFATLLQGLPVLPKEWWDPKWIKDNIQPKLDP
                     STVVNSEKEKPVRKVTLKRRR"
     gene            complement(1806..2291)
                     /locus_tag="VFN58_02610"
     CDS             complement(1806..2291)
                     /locus_tag="VFN58_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8916913.1"
                     /translation="MNQGNREINARESSMAAQSELTGRYNCVADVMTRKTVSLSPHHG
                     LVEAVSLMADRHFRHIVVVDTGGQILGVISDRDILRTLARTSNWQTKDVSQIMTRDPF
                     TVKRETPLSVAVAKIVAKRINCLPVLDDEGKVCGIVTSTDLLKAYQKLLESLQRNRAA
                     L"
     gene            complement(2344..2829)
                     /locus_tag="VFN58_02615"
     CDS             complement(2344..2829)
                     /locus_tag="VFN58_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8916914.1"
                     /translation="MEIAVATDLAAGHPGRPSSATRVADIMSSKLVTLSAHHTFGQAV
                     QLMTNSPFRHFLVIHADGRLAGLFSDRDVLRALGRTPNWQAKNVSGVMTHKVFTVTPE
                     TPLSVAASEMLSRRINCLPVVGNDGKVCGIITSTDLLRSYQKIQAALENDSCGTSAGS
                     V"
     gene            3172..>3929
                     /locus_tag="VFN58_02620"
     CDS             3172..>3929
                     /locus_tag="VFN58_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET8916915.1"
                     /translation="MTLILNNDDIQKALNVKECLEVMEECYLEQAAGRAVNRPTCHSY
                     LPHSIPQSTYSFKSVDGGVGKFGVLALRITSDVVQEQQVHSSVRLEKLPLAGTGMFVG
                     LVQLFSAVTGELLAIMPDGAIQQTRVGVTSALGMKAMARKNSEILGLIGSGGQARAHY
                     RFMTSVLGIKRVKVFSPNPEHRKQFTAEMADETGVAGEPVNSAEEATKDCDVVCSATN
                     SSRPVLNPDWLQPGMHYNSIREFETDLAALDRCDV"
BASE COUNT         1005 a         1073 c          991 g          860 t
ORIGIN      
        1 ggtcatattc gttcacaatc acccgagcgg cgaccccgct cccagccaag aagacatcga
       61 aatcacccgc aggctcaaag aagtcggtga ggtcatgggc atccgagtgc tcgaccatgt
      121 cgtgatcggt ctcgagcgtt attacagctt cagtgacaga ggaatgctct agcctacggt
      181 ggctagttac accagtcatt aacctgaaac tcacctaatg agttggtttc atcggattta
      241 acccgctata ttttattgac ttcgacctgg ccgacgttta atattctggt tcaacaagtt
      301 agacgctccc gtccttttat caccagagca tgaccgccct agaactaggt agacccccaa
      361 gaagaacaca cgttcttaga aatatcatca ttgttaccct tttggcggcc atcattgctg
      421 gaggttactt cctggtaccc aagtttgaat ggtacaaacc gcaagtcaaa atcacaccgg
      481 acacggatac cctgggactc gctcccatcg aaatagaaat caatgaggcc ggcaccggtc
      541 tgaagtccgt tgccgtaacg ctttctacgg gcggcaccga acgccctctc ttctcgcaag
      601 aatatgatcc gccggtcatg caagcgaaag tgacggtcgc ctcatcgaag ttatccggca
      661 tcaaggaagg tccagcgata ttccgcgtga gcgcgcgtga ccgctccttg tggagctttt
      721 ttagaggcaa cgagaccgtc atccagaaaa acgtcaccat cgatatcacg ccgccggcgc
      781 tggagcttat cgcagacgat ccttacgtca acttcggtgg ggtcggactg gtagcatata
      841 aaacctcatc cgatgcggtg acaagcggcg tgaagatcgg cagctatttt tttcctggtt
      901 ataaggggca aaccaaggac cccgatacct atatctcttt cttcgctcac ccctataacg
      961 tatccgaagg cgaaaaggcc gtcctggtgg ccaccgacaa ggccggcaac tcacgggagc
     1021 tgcggcttgc ttataccctc aaggatgtca aatacaaaaa aagcaccatc cctgtttccg
     1081 acgatttcat ccagggcaag gtcgcgcccc ttttggccga cgtcggggca cgccagggaa
     1141 gtccgaagga tatttttgtc aaggtgaatc atggtctgcg caaagaaaac gaagacaaga
     1201 tccgcatggt gggtcaaaaa accgccaatc gcatgctttg gagcggtccc ttcattcagc
     1261 tttccaattc caaggtggag gccaacttcg ccgactcgag aacctacatc tatcgcgatg
     1321 aaccgatcga cacggcctat catcttggct ttgacctctc cgtaaccaag agatatcccg
     1381 tggaagcagc caacagcggg gtcgtaagct tcgtcgggga tttggggatc tatggtaaca
     1441 ccgtcatcat cgaccacggc ctcggactct ctacccttta cggccatttg agttccgttg
     1501 acgttaaaga gggcgagcag atcaagcagc aacaaataat cggcaagacc ggcgaaacgg
     1561 gtcttgccgt aggcgaccac ctccacttcg cgacgctgct acaaggactc cctgtattgc
     1621 cgaaggaatg gtgggatccg aaatggatca aggacaacat ccagcctaaa ctcgaccctt
     1681 caacagtggt gaacagtgag aaagaaaagc cggttcggaa agtgacgctg aaacgtcgcc
     1741 gctagtcatc cgtcgcaatg tctacttcat tcatcccagc atctggccat caaagacagg
     1801 tgatgtcata gagccgcgcg attacgctga agcgactcca gaagtttctg ataggctttc
     1861 agcaagtcag tggacgtgac aatcccgcac accttcccct catcatcaag caccggcagg
     1921 caattgattc gcttggcgac tatcttcgcg accgcaacgg aaagcggggt ttcacgtttg
     1981 acggtaaatg ggtcgcgcgt catgatctga ctaacgtcct tcgtttgcca gttgcttgta
     2041 cgcgccaaag tacgcaagat gtcacgatcc gaaatgacac cgagaatctg tcctccggta
     2101 tctaccacga cgatatgacg aaagtgacgg tcggccatca aactcaccgc ctcgaccaag
     2161 ccatgatggg ggctaaggct gaccgtctta cgggtcatca cgtcagcgac gcagttatat
     2221 cttccagtca attcactttg cgccgccatt gaagattcgc gcgcgttgat ttcccggttc
     2281 ccctgattca taccgagacc tccttaaaga aatctatcgg agaaaacaag cccatcctca
     2341 aaatcaaaca gaacccgcac ttgtgccgca cgaatcattt tccagcgcgg cttgaatttt
     2401 ttggtagctt cgcagcaggt cggtcgaagt tatgatgccg cacactttgc catcgtttcc
     2461 aacgacggga aggcagttga tacggcgcga caacatttcc gaggcagcca ccgaaagagg
     2521 tgtttcgggc gttaccgtaa atactttatg cgtcatcacg ccgctcacat tcttagcctg
     2581 ccaattgggg gtgcgcccga gagcacgcaa aacatctcga tcggagaaaa gaccggcgag
     2641 ccgtccatcc gcatgaatga ctaaaaagtg gcgaaaagga gaattggtca ttagctggac
     2701 tgcctggcca aaggtatgat gtgcagatag agtgaccagt ttgctgctca tgatgtcagc
     2761 cacgcgggta gctgaggacg gccggccggg gtggccggct gcaagatcgg ttgccacggc
     2821 gatttccatg ttgtgctcca taaaattctt cgagcaaatt tattatcgta aaatcaaaga
     2881 gttgcggaaa tgcagagata cacgcgcgcc caagaaccca attaagtcca tatccccttc
     2941 tgtcttatcg gcgggaaagc aggagacttt acggactcgc ataggaaact aaagttatct
     3001 aaatggtcgt gagccgtatg agcggccgga cccactttgt ctttcgtaga gtccgcccca
     3061 aattctgagt atcccccaaa ctcattagcg aaacccagtc aaccgattgg acaattatac
     3121 atcgatcgga taggaatggc actctaaaca atcgctagcg ccggggaaga gatgactctc
     3181 attctaaaca acgacgatat ccagaaagca cttaacgtaa aggaatgcct cgaggtcatg
     3241 gaagaatgct atctagagca agccgctggc cgtgcagtca accggccaac ctgccactcc
     3301 tatcttcccc actcgatccc gcaatccaca tacagtttta aatcagtcga cggcggcgtc
     3361 ggcaaattcg gcgtgttggc cctgcgtatt acctctgacg tggtgcagga acaacaggtt
     3421 catagttccg tgcgcctgga aaagctgccg cttgccggaa cgggcatgtt tgtgggtctt
     3481 gttcagcttt ttagtgccgt gaccggcgag ttgttagcga tcatgcctga cggagccatc
     3541 caacagaccc gagtcggcgt caccagcgct cttggaatga aagcaatggc gcgcaagaac
     3601 tccgaaattc tagggctcat aggttccggc ggccaagcgc gcgctcatta ccgtttcatg
     3661 acttccgtac taggaattaa gagagtgaaa gttttcagcc cgaacccgga acaccgaaaa
     3721 caatttactg cggaaatggc ggacgaaacg ggtgtggccg gagagccggt aaatagcgcg
     3781 gaagaagcga ctaaagactg cgacgtcgtg tgcagcgcga cgaactcatc tcggccggtt
     3841 ttaaaccccg attggttgca acccggcatg cattacaact ctattcgaga attcgagacc
     3901 gacctggccg ctttggacag atgcgacgt
//
LOCUS       DASSWZ010000105         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1290825, whole genome shotgun sequence.
ACCESSION   DASSWZ010000105 DASSWZ010000000
VERSION     DASSWZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290825"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFN58_02625"
     CDS             <1..419
                     /locus_tag="VFN58_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HET8916916.1"
                     /translation="LRVPLAAKLLRQPDPERSIIVTSDRSPHQKARAIESLGAQVWRV
                     RLRRGRIPWTAILRRLAAKGTLSVMIEGGATTAAWALQEQAVDKLLFFYAPMILGGDG
                     RVMIDALGVKRVRQALRVQRTRVRKLGTDTLVSAYL"
     gene            486..1073
                     /locus_tag="VFN58_02630"
     CDS             486..1073
                     /locus_tag="VFN58_02630"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET8916917.1"
                     /translation="MFTGLIEDVGRIASLRLNKGSAVLAVETSLPVRSMELGASIAIN
                     GACLTVVKKDKSAFTVNVSPETLNRTSLAMLGIGGLVNLERPLRLQDRLGGHLITGHI
                     DGVAVVESIETLGEFTFFTFHVPVQLGTFLVSKGSVAVDGISLTVNECSRRRFAVAII
                     PFTLEHTNLRARRVGDKVNIETDLVGKYVQKFVKA"
     gene            1120..2346
                     /gene="ribA"
                     /locus_tag="VFN58_02635"
     CDS             1120..2346
                     /gene="ribA"
                     /locus_tag="VFN58_02635"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET8916918.1"
                     /translation="MSLATIEEGIKELREGRMVILVNEERPDSDGFFCIPAEKVTSEI
                     INHMMHHGRGIIYVTLTDERIRELGIPLVPEENSSLAGLLCGASFSVRLEGVHGVSAE
                     GRAHTIQAAIANQTKHEDLVIPGHVQPLQARSGGVLARSGRTDASVDLARLAGFKSAG
                     VICQILEDDGSLALLPYLEGLAERQGIRIVSVTGLIAFRLRNESLVKRVAEEVFPTIH
                     GGVYKAIVYRNEVDGSEHMALVKGDIGDATGVLVRLHSECLTGDVFGSERCDCGDQIR
                     QSLKLIDQQGKGVLVYMHQEGRGIGLTNKIKAYALQDKGLDTVEANLELGFKEDLRDY
                     GIGAQMLRDLGVQSIRLLTNNPRKILGLEGYGLKVEERVPLEIPPRDTNIHYLRTKQS
                     KLGHIFSNLESKPAKH"
     gene            2369..2839
                     /gene="ribE"
                     /locus_tag="VFN58_02640"
     CDS             2369..2839
                     /gene="ribE"
                     /locus_tag="VFN58_02640"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET8916919.1"
                     /translation="MANIIEGKLDARGIKIGIIVSRFNNFITEKLLDGALEGLKSHGG
                     EENDVTVIRVPGAFEIPLLAERMAASGKYDALVCLGAVIRGDTPHFEYVSDAVTRGIG
                     EAISKYRIPIGFGVLTTNNVEQAMERSGSKDANKGYEAALTAVEMINVIRRLSE"
     gene            2904..3320
                     /gene="nusB"
                     /locus_tag="VFN58_02645"
     CDS             2904..3320
                     /gene="nusB"
                     /locus_tag="VFN58_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET8916920.1"
                     /translation="MGTRRKGRELALQALYQIEITGDGSVAAMELFLSHFEGSAEAKE
                     FARRLVSGVMSQRVEIDRLIQQSTEHWKLLRMAKVDLVILRMATYELVFCPDIPLNVS
                     LDEAIEIGKRFGTDDSPTFINGVLDQVAHSGYVKSQ"
     gene            3330..>3584
                     /locus_tag="VFN58_02650"
     CDS             3330..>3584
                     /locus_tag="VFN58_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET8916921.1"
                     /translation="MEDRYRAKAIEEKWQQIWEENQTFQTSEQAGKPKYYVLEMFPYP
                     SGRIHMGHVRVYAIGDVIARFRRMRGYNVLHPMGWDAFGLP"
BASE COUNT          848 a          836 c         1044 g          856 t
ORIGIN      
        1 gtcttcgcgt ccctctcgca gcaaagcttt tgcgtcaacc tgaccctgaa aggagcatta
       61 tcgtcaccag cgatcggtcg ccgcatcaaa aagcccgtgc aatcgaatcc ttgggagcgc
      121 aggtttggcg ggtgagactg cgacgtggaa gaattccttg gacggcgatc ttgcggagac
      181 tcgcggcgaa gggcacgctc agcgtcatga tcgagggcgg ggcgacaact gcagcgtggg
      241 cattgcaaga acaggcggtg gataaactct tgtttttcta cgcgccgatg attttaggcg
      301 gcgacggccg ggtcatgatt gatgctctgg gagtcaaacg ggttaggcag gcgctacgtg
      361 tccagagaac gcgagtgcga aagttgggga ctgataccct tgtttccgca tacctttgaa
      421 acgaaccgaa tctagggatt cccttgccgg cggtagagct tgagtaaaca ttaattctga
      481 aggttatgtt tacaggttta atagaagacg tgggaagaat tgcgtcgctt cggctgaaca
      541 aagggtcggc ggttttggcc gttgagacca gccttcccgt gcgctcgatg gaacttgggg
      601 ccagtatagc catcaatgga gcatgtctga ccgtagtgaa aaaagacaag agcgcgttta
      661 cggtgaacgt ctcacctgaa acactaaatc gcacgagtct cgcgatgctc ggaatcggcg
      721 gcttggtcaa tctcgaacga ccattgcgac tccaagaccg gttagggggc catttgatta
      781 ccgggcacat agatggggtt gcagttgtcg aatccataga aacgcttggt gagtttacct
      841 tttttacgtt tcatgtccct gtgcagttag gaactttcct ggtatcgaaa ggttcggttg
      901 ctgtggacgg gattagcttg actgtaaatg aatgtagccg tcgacgcttt gcagtcgcga
      961 ttatcccgtt caccttggag catactaatt tgcgggcccg tcgcgttggt gataaagtca
     1021 acatcgaaac ggatctcgtc ggaaaatacg tgcaaaagtt cgtcaaggcg tagaattagt
     1081 ttcagctttt cagccatttc gcacccctgg ttctagacta tgtcgctggc aaccattgag
     1141 gagggtatca aggaactccg cgaaggtcgg atggttatcc tcgtcaacga ggagaggccc
     1201 gacagcgatg gtttcttttg tatccccgcg gaaaaggtta ccagcgagat catcaatcac
     1261 atgatgcacc acggtcgagg aattatctac gtaacattga ccgatgaacg gattcgggaa
     1321 ttgggtattc ctctggtccc ggaggagaac agcagtttgg ctggcttgct gtgcggcgcg
     1381 tcgttttcgg tcagattgga aggtgtgcat ggcgtgtcgg ctgagggtcg agctcacacg
     1441 attcaggcag ccatcgccaa tcaaaccaag catgaagacc tggtgattcc tggccatgta
     1501 caacccctcc aggcgcgcag tggtggagta ttggcgcgtt ctggccgaac cgatgcttcg
     1561 gtggatctgg cccgattagc ggggttcaaa tctgccggcg tgatctgtca aattctggaa
     1621 gatgacggtt cccttgcctt gcttccgtat ctggaaggct tggccgagcg gcaaggcatt
     1681 aggattgtgt cggtcactgg attgattgcc tttcggttgc ggaacgagag tctggttaag
     1741 cgcgtggctg aggaggtgtt cccaacaatc cacggtggcg tgtacaaagc cattgtctat
     1801 cgcaacgagg ttgatggttc tgaacatatg gccctcgtga agggggatat cggagatgcg
     1861 acgggggtgc tggtgcggct gcattcggag tgtcttacag gcgatgtttt tggttccgag
     1921 cgttgcgatt gcggtgatca gatacggcag tctctaaagc taatcgacca acaaggtaag
     1981 ggcgtgttgg tttacatgca tcaggaaggc aggggcatcg gtcttaccaa caagatcaag
     2041 gcctatgcct tgcaagataa aggtttggat acggttgagg ctaacctcga actaggcttt
     2101 aaagaagatc ttcgggacta tggcatcggc gcccagatgc tccgcgatct cggcgttcaa
     2161 agcatccgct tgttgaccaa caatccgcgc aaaatcctcg gcctcgaagg gtacggactg
     2221 aaagtggaag aacgggtgcc tctggagatt ccgcctcgcg acaccaatat ccactatctt
     2281 cgtacgaaac agagtaagct cgggcatatt ttttccaatc ttgaatcgaa gcccgccaaa
     2341 cactgacccc ctttggtgcc gcagactcat ggccaatatt attgaaggca agcttgatgc
     2401 acgggggatc aagatcggta ttatcgtcag ccggttcaat aatttcataa cggaaaaatt
     2461 gctggacggt gctttggagg gactgaagag ccatggcggt gaagagaacg atgttaccgt
     2521 tatccgcgtt cctggcgcat tcgaaatacc tttgctggcg gagcggatgg cggccagcgg
     2581 aaaatacgat gccctggttt gcttaggcgc cgtgattcgg ggggatacgc cgcacttcga
     2641 atatgtatcc gatgcggtaa cccgaggaat cggggaagcc atctcaaaat atcgaattcc
     2701 catcggtttc ggggttttga caaccaataa tgtcgagcaa gcaatggaac gctccggttc
     2761 caaggatgcg aataagggtt atgaggcggc cctgacggcc gttgagatga taaatgtaat
     2821 tcgtcggctc tccgagtaag tgggcactcg gcattgttga tccgtcgctt ctaagaagct
     2881 ttttgacaaa ggcattgtat atcatgggaa ccagacgaaa gggcagggaa ctggcccttc
     2941 aagctttata tcagatcgag ataaccggtg acggttcggt tgctgccatg gaactttttc
     3001 ttagtcattt cgaaggcagc gcagaggcaa aggaatttgc ccgtcgtttg gtgtcgggag
     3061 taatgagtca gcgtgtggaa atcgatcgac tcattcaaca gtccaccgaa cactggaagc
     3121 tattgcgcat ggcaaaggtt gatctggtga ttctacgcat ggcgacttac gagctggttt
     3181 tttgcccgga catcccactg aacgttagtt tggatgaagc catcgagatc ggcaaacgct
     3241 ttggcacgga cgactcccca acctttatca atggcgtgct ggatcaggtg gcccactcag
     3301 gctacgtaaa atcacaatag atttagaaca tggaagaccg ctatcgggct aaggccatcg
     3361 aagaaaagtg gcagcaaatt tgggaggaaa accaaacctt ccaaacctca gagcaagcag
     3421 ggaaacccaa gtactatgta cttgagatgt ttccctatcc ctccggacgc atccacatgg
     3481 gccatgtgcg cgtatatgcg attggcgacg tgatcgctcg ctttagaagg atgaggggct
     3541 acaacgtgct ccaccctatg gggtgggacg cctttgggct tcct
//
LOCUS       DASSWZ010000106         6193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_257913, whole genome shotgun sequence.
ACCESSION   DASSWZ010000106 DASSWZ010000000
VERSION     DASSWZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257913"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFN58_02655"
     CDS             complement(<1..314)
                     /locus_tag="VFN58_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8916922.1"
                     /translation="MNPSRPFILRPIATSLLMAGLLLIGAVAYKQLPVSALPEVDYPT
                     IQVVTFYPGASPDVVASSITAPLERQFGQVPGLNQMTSTSSDGSSVITLQFSLDLNID
                     VA"
     gene            complement(328..1503)
                     /locus_tag="VFN58_02660"
     CDS             complement(328..1503)
                     /locus_tag="VFN58_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HET8916923.1"
                     /translation="MPRQGLASGHKRYWIGLIVIAFLGVGTYVYFVKPGEADLPAARQ
                     RQNSGARPVSVVTAKAKAGDMPVYLSGLGTVTPLNTVTVKSRVDGQLMKVSFKEGDLV
                     RSGDLLAEIDPRPFQVQLTQAEGQMAKDQALLKNAQVDLERYQVLFNQDSIPQQQLAT
                     QESLVSQYEGAIKVDQGQIDNARLQLTYCRITAPITGRVGLRLVDPGNIVHAADTNGL
                     LVITEMDPIAVIFAISEDSLPQVQQQMRGGHPLPAEAYDRDLKHKLASGTLLTIDNQI
                     DPTTGTVKLKALFPNTNNTLFPNQFVNVRLLVDTIRNAVLVPNAAIQHSPKSAFVYVV
                     KPDNTVEARNVEVQGTERDQTAIAHGLMAEEVVVTDGVDKLQPGAKVALSMPQPAGS"
     gene            complement(1644..2333)
                     /locus_tag="VFN58_02665"
     CDS             complement(1644..2333)
                     /locus_tag="VFN58_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HET8916924.1"
                     /translation="MFENEAKTADLVPGTEKRLLEAAGEIFAEHGYRAATVRQICEKA
                     RANIAAVNYHFGDKEGLYRAALRSVHRASAEKYPSNLGVSPATPPQQKLRVYVRSLLL
                     RIFDEGRPGWHMKIMMREMLEPTRALDLLVEEGARPLHQKLFSIVRELLGSAADDEVV
                     RLSALSIVSQCVYYRRARPVITRLYPQQRYDSKEVERLTEHITQFSLLAIEGLCKKMP
                     GEVHGTVEADG"
     gene            complement(2486..2836)
                     /locus_tag="VFN58_02670"
     CDS             complement(2486..2836)
                     /locus_tag="VFN58_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916925.1"
                     /translation="MRDFRQRRIARGAILIFAVVLLSSSFAAYRHFSTGVAKYITYDH
                     ALRRVLGRAERDNLRSGLMVKEQGPMAERPQQIGEVPPSPQLVATYCRISFVEHSLID
                     PNRLVHRLSPVFNL"
     gene            complement(3120..3623)
                     /locus_tag="VFN58_02675"
     CDS             complement(3120..3623)
                     /locus_tag="VFN58_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8916926.1"
                     /translation="MNVAERFIRRPVVTILMMLGILLLGVTGYLFLPAVKPRNLDSPT
                     ILVSANLPGASAAIMESSVATPLERQFVTLTGLEAMTSVNDVGFTDIALEFNPNRNID
                     TAAQDVQEAITRTLPQLPAGMPNPPSCQKLNRADLALLDFLSTIDKSVESVIAQTGSS
                     FRDSSHA"
     gene            complement(3825..4124)
                     /locus_tag="VFN58_02680"
     CDS             complement(3825..4124)
                     /locus_tag="VFN58_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916927.1"
                     /translation="MKIVRPIFVALLFIGTMGSYNPAFAANGIILEQEMTPGSYCHLK
                     FPAIREETLAGDHPVLKVPNEGNLIDFYGPCDEDPVEKDQVHEQRIENQHRWEFD"
     gene            complement(5228..5419)
                     /locus_tag="VFN58_02685"
     CDS             complement(5228..5419)
                     /locus_tag="VFN58_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET8916928.1"
                     /translation="MLGDIGFPELLVIFIIVLVLFGPGKLPDVGKAIGEALRGFKKAL
                     NEPQKPIAERSNKEDEKSR"
     gene            complement(5422..>6193)
                     /locus_tag="VFN58_02690"
     CDS             complement(5422..>6193)
                     /locus_tag="VFN58_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET8916929.1"
                     /translation="GNSGGPLVNLQGEVVGIDTAIFSQSGGNIGIGFAIPINLVKDLL
                     PQLKSGGKITRGWLGVSIQGITPDLAASLGLDQVKGALVSSVVQNSPADRAGIRAGDV
                     IVGYEGKAINNAKDLPTLVAGTPVGKTVSLQVLRGEKETPVAVAIEKMKEEEVIASTT
                     ETGDLGLTVEQITPEIANDLGLEQTHGVVVTAVAPDGPGDEAGFQPGDIVREINRQPV
                     RNLSDYRKIMASAAHGKSILFLVQREDNAIFLALRKEE"
BASE COUNT         1403 a         1635 c         1631 g         1524 t
ORIGIN      
        1 tccgctacgt cgatgttgag atccaggctg aattgtaacg tgatgaccga gctaccgtcg
       61 gagctggtgg acgtcatctg gtttaaaccg ggcacttggc cgaactggcg ctccagcggc
      121 gctgtgatgg acgacgctac cacatcgggg ctcgcgccgg gatagaaggt gacgacttgg
      181 atcgtcggat agtccacctc cggcagcgca gacaccggca gctgcttgta ggcaaccgct
      241 ccaatgagca gcaaacctgc catcaacaac gaggtcgcaa tcggtctgag gataaacggt
      301 cgagagggat tcattggcaa gtctccttta agagccagcc ggttgaggca tgctcaaagc
      361 aacctttgct ccgggttgga gcttgtcgac tccgtcggtc acgacgacct cttccgccat
      421 aaggccgtgc gcgattgctg tctggtctct ctcggttcct tggacttcga cgttgcgggc
      481 ctccacggta ttgtccggtt taacaacgta cacgaacgcc gacttcgggc tgtgttggat
      541 cgcagcgttc ggaacgagga ctgcgttgcg gatcgtatcc accagcagac gtacattaac
      601 gaactgattt gggaagaggg tattattcgt gttcggaaac agcgccttga gcttgactgt
      661 accggtcgtc ggatcgatct gattgtcgat cgtcagcagc gttcccgagg cgagcttgtg
      721 cttcagatcg cggtcgtaag cttcggccgg taacgggtgt cctcctcgca tctgctgttg
      781 gacttgagga aggctgtcct cggatatcgc aaagatcacg gcgatcgggt ccatctcggt
      841 aataacgagc aatccgttcg tgtcggcggc gtgcacgatg ttgcccggat ccacaaggcg
      901 gagtcccacc cggccggtaa tcggcgctgt gatgcggcaa taagtgagct gcaatctggc
      961 attgtcgatc tgtccctgat cgaccttgat cgcgccttcg tactggctca cgagggattc
     1021 ctgagtggct aactgctgct ggggaataga gtcctggtta aatagcactt gatagcgctc
     1081 caggtctacc tgggcgtttt tgagcagtgc ctgatctttc gccatctgtc cctcagcctg
     1141 ggttaattgt acttgaaaag gacgtgggtc gatttccgcc aacaaatcgc cgctgcgaac
     1201 aagatcgcct tccttgaagg agaccttcat gagctggccg tcaactcggc ttttaaccgt
     1261 tacggtgttg aggggcgtga ccgtgccaag gccggagaga taaacaggca tatcaccggc
     1321 tttggccttt gccgtcacga ctgaaactgg acgcgcgccg gagttttgac gctgccgagc
     1381 agccggcaaa tccgcttcgc ctggtttcac gaaatagacg taggttccca ctcctaaaaa
     1441 agcgatgacg atcagtccaa tccagtagcg cttgtgcccg ctcgccaaac cttgtcgtgg
     1501 catatctttg ttctctcctc gtgcgtagca aacgtggcgc ccttcgagcc agtgagctaa
     1561 aacgccggca acgagtgcct tcgatcaccg ctatctagac ttttcgtcat aactgccgtt
     1621 gctgaaaaaa ccgtttccac cttctaccca tcagcctcta cagtgccatg aacttctcct
     1681 ggcattttct tgcagagacc ctcgatcgct aaaagagaaa attgcgtaat atgttctgtc
     1741 aatctctcga cttccttgga gtcatatcgc tgttgtgggt acagtcgggt gatcactggt
     1801 cgtgcacgcc gataatagac acattgactc acaatgctga gggcgctcaa gcgaacaacc
     1861 tcatcgtctg ccgctgaacc gagaagttcc cggacgatgg aaaacagttt ctggtgaagt
     1921 ggacgggccc cttcttccac aagcagatca agagcacgtg tcggttcgag catctctcgc
     1981 atcatgatct tcatgtgcca acccggacgc ccttcgtcga agatgcggag caatagcgac
     2041 cgaacataga cacggagttt ttgctgtggg ggggtcgctg ggctaacgcc gagattggac
     2101 ggatatttct ccgcactcgc tctgtgtact gagcggaggg ccgccctata aagtccctct
     2161 ttgtcaccaa aatggtaatt gacggcagca atattagctc tggctttttc gcagatctgc
     2221 cttactgtgg ctgcgcgata gccgtgctcg gcgaaaattt caccggcagc ctctaagaga
     2281 cgcttttccg tcccgggaac caaatctgca gttttcgctt cgttctcaaa catatgtttc
     2341 aagcaactgt attagagtcc acaaacatct tggtggtgct ctcgcacctg gtcacaaaaa
     2401 ctcgacgatg tcacccaaat tggggaaagt gacagcaccc aatcaagctg atggcttgtt
     2461 ggatgactcg agtagggcta aaaaatcaga gattaaaaac cggagataac ctgtggacta
     2521 agcggttggg gtcgatcagc gaatgctcta cgaaagagat tctacaatat gtggcaacga
     2581 gttgaggtga tgggggaacc tcgccgattt gctgtgggcg ctcagccatc gggccttgct
     2641 ctttcaccat cagaccgctt ctgaggttat ctcgttcagc cctgccgaga acacggcgca
     2701 gcgcgtgatc ataggtaata tatttcgcca ccccggtgga gaaatgtctg tatgctgcga
     2761 agctgctgga caagagcacg acggcaaaaa taaggatcgc cccgcgcgca attcgccttt
     2821 gcctaaaatc cctcataacg aaagtatgtt aaatcccacc ggtataatgt caaggtccta
     2881 ataaccatga ttgcacattt tagagatcac atcttcttgc ctcacgccaa ttttgattgg
     2941 caagccctgt gccaaacccg gcttagcaag ggtgagtctc gcgcgccacg gacaattaag
     3001 aatcgataag aactgggtcg cgtattttgg actgcaattg ggtcatgccc tgtggcagaa
     3061 atgtgtgatg ggtgtggctt tcttgcaaga gccagagagc tttcttaggg aaccggacac
     3121 tacgcatggc tggagtctct aaaagaggag ccagtttggg caatgacact ttctactgac
     3181 ttgtctatgg ttgacaggaa gtcgagcaaa gctaagtcgg cgcgattcaa tttctgacac
     3241 gacggaggat tgggcatgcc agcgggaagc tgcggcaagg tacgagtgat cgcctcctga
     3301 acatcctgag ccgcggtgtc gatgttgcgg ttcggattaa attccagcgc gatgtcggtg
     3361 aagcccacgt cattgacgga tgtcattgcc tccaatccgg tcagggtcac gaactggcgc
     3421 tctaacggcg ttgcgaccga cgattccatg attgcagcgc tagcgccggg aagattggcg
     3481 gaaaccagga tagttgggga atccaagttt cgcggcttaa ctgcaggcaa gaacaagtag
     3541 cccgtgacgc caagtaacag aattcccagc atcataagga tcgtcacgac aggtcggcgg
     3601 ataaaccgct ccgcgacgtt cataaactcc ttttcccgct actttactgg cggttttgaa
     3661 acgagaaact ggacaaataa acacccggtg actgatgaag cgtaccggtt ccgtactgat
     3721 agtttccaca acacggatca cgagcaacga tcgtaccaag gtgctacttg ccgttatcgc
     3781 cagacgggaa ttgcagactg ccttgcgtcg gctctcattt aactctagtc gaactcccat
     3841 cgatgctggt tttcaattct ctgttcatgc acttggtctt tctcaacagg atcctcgtca
     3901 catgggccgt agaagtcgat gaggttccct tcattcggaa ctttcagaac cggatgatct
     3961 ccagccaacg tctcttcgcg gatcgcagga aacttcaggt ggcagtaact gcctggagtc
     4021 atttcctgct ctaaaatgat gccgttcgcc gcaaacgctg ggttatagct tcccatcgta
     4081 ccgatgaaaa gcaaagccac gaaaattggt ctcacgattt tcatgcacat cctattcatc
     4141 ctgcttcctc ctaataatga tttgggcaat gcgcgtgcca aaccgtatcg acgttcataa
     4201 agcgcgtatg gagataacga ggatcagaaa ggttacagaa attctgctag agagtccgac
     4261 tcgatcggca atgaggccgg gatgtgtgat ctgctgtagc tttcttgcgg tatctttgtc
     4321 agggctgggt gaaaagtagt tgttcgcctt tcgaccagca ccgggtcgag tgagaaatcg
     4381 ctgagtccga ttcccttaca aggcgtgttg acggcaagca gacgctaaac cgaggcgaga
     4441 gcgctgcaaa cagttccgtt gttttttact tttccgtcgt caagaggttc aacggcaatt
     4501 ccgagtctcc gggcttcatc ggcacaggcc gcacatacct tcatgtctat tacatcagta
     4561 taaacccggt acttggcttc tttgcctagc aggcaccgtt ggcattccat tgggatctcc
     4621 ttaaaaggct ggtcgcctat gcgactgtcc tgcgttcgta ttcgaaacga cgcggcgcaa
     4681 ccagttcaag acgtggtcgg tctttgcgcg gaagtacgtg ttgcgtcttt cggatataac
     4741 gcagccgctc ctggaaccaa gtttgctgtg gccagtccca attttgccgc gggagcctat
     4801 ctccgtcata tgcgccgctg ttcactttgc tttgaagaaa aatacgctgc cagtagctca
     4861 taaccatttc tcctttcgca atatcaaagg gcaaggaatg tgccagcttt gccaagagct
     4921 aacagaaaca agaaagggaa tcagtttgaa ggcagcaagt gttcgttagt gactcgtcac
     4981 aaaactatcg tgtcgaaata tcggcgtatg tctaaatacc gatagtcggt cagtcttcgt
     5041 cgtaatagtg gtgtggccaa tgagtctggt atttttggca agcagcttga tcgtaacttt
     5101 gcgcagctat acaaccccat ccctctagga aagtggcgac taacatgatg gctacaaaaa
     5161 ctgaggttgc gattttaatt agatgaatgt ttgttctcct cagcgctgga cattctttgc
     5221 agtcacatca acgtgacttc tcatcttctt tattggatcg ttcggcgatg ggtttttgcg
     5281 gttcattcaa agctttttta aaaccgcgga gcgcctctcc aatcgctttt cccacatctg
     5341 gaagctttcc cgggccgaat aggacaagca cgataatgaa gatgacaagc agttctggaa
     5401 agccgatatc accgagcatc gctattcttc tttccggaga gccaggaaaa tcgcgttatc
     5461 ttcgcgttgc accaggaaca gtatgctctt gccgtgtgcg gcgcttgcca taattttcct
     5521 gtaatcggac aggttgcgta ccggttgacg attgatctcg cgaacgatgt cgccaggttg
     5581 gaagccggct tcgtctcccg gaccgtccgg agcgacagcg gtgactacca cgccgtgggt
     5641 ttgttccaga ccaaggtcat tggcaatctc cggagtaatt tgctcgaccg taagtcccag
     5701 gtctccggtt tcggtcgtcg aagcgatcac ttcctcttcc ttcatttttt cgatggcaac
     5761 ggcgaccggt gtttccttct cgcctcgaag gacttgaagg gaaacggtct tacccactgg
     5821 cgtacccgcc accagagtgg gcaggtcctt agcattgttg atcgctttgc cttcgtatcc
     5881 gacaataacg tctcccgccc tgatcccagc gcggtctgcg gggctgttct gtacgacgct
     5941 gctaacgagc gcacctttca cttgatccaa cccgagggac gcagcgagat ccggagttat
     6001 cccctgaata gacacgccaa gccaaccccg cgtgattttt ccgccgctct tcagctgggg
     6061 tagtagatcc ttcaccagat tgatcgggat ggcaaagccg atgccgatat ttcctcccga
     6121 ctgactgaag attgccgtgt cgatgccaac gacctcgcct tgcaaattga ccaaaggtcc
     6181 gcctgaattg ccg
//
LOCUS       DASSWZ010000107         7978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_102145, whole genome shotgun sequence.
ACCESSION   DASSWZ010000107 DASSWZ010000000
VERSION     DASSWZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102145"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..863
                     /locus_tag="VFN58_02695"
     CDS             171..863
                     /locus_tag="VFN58_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET8916930.1"
                     /translation="MSNDRLPAHLRALKIGAQSMGALAIGALAIGALSIGALAIGRLV
                     IGRSRIRRLEIDELVVARLHVTASVPSTPATKNDPPTKETKESFNQPATFARALNKLF
                     GFFVGLGFGLPHNYLLQVRGRKSGRVYATPVDVLNRAGKRYLVAPRGHTQWVRNALAS
                     GIVSLKKGRRSEEFRIRILSDDEKPEILKAYLDRYKLTVQRYFPVPAGSATDEFRPLT
                     GNYPVFELLPIP"
     gene            complement(860..2005)
                     /locus_tag="VFN58_02700"
     CDS             complement(860..2005)
                     /locus_tag="VFN58_02700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8916931.1"
                     /translation="MKVIDADAHMHEPEYLWERYVEPKFRDRVPKVAFMDGVFMVYEP
                     DGKIIPKGDLQLRPPPSAWAVLEEKYGEAYRQWWSPDIRLKDMDRYGWDMQVLLPTGN
                     NGNFAYRVALKDVELGAAMCRAYNNWCRDYCSADSKRLKFVAVLPGSDVGEMVKEARR
                     AVVELGAVSVRNPFLPEGKYLHEPEYDALWEAACELDFPISCHGEYRQRRFHPFRHLE
                     QERREDLQLALRGLDHALGFPCDNMTTLGHFIFTGILERFPKLRLAILESNAGWLPFW
                     LGRMDDHTHGRNSVMGKSEKLRLRPSDYFMRQCMISCDSDERALKYVVDDLKGENIVW
                     NTDYPHPDAIDPKRALPDLDAQPISDDAKRKILWDNAVKLFGPRILG"
     gene            2155..3225
                     /locus_tag="VFN58_02705"
     CDS             2155..3225
                     /locus_tag="VFN58_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916932.1"
                     /translation="MDIRLARKDKSAVIFTGEWRRMAMRQWVFVKPKNFVIFFPLSWL
                     LLWTSAARSADQIRVGYGSLSTSYAAIWVAGEAGLFRKNGINAEVLYLESALVRTALI
                     AGDIAMGGMSGTTMAAPRLQGADSVIIASFANSLQYRFVVRPDIRTVADLKGKRVGVA
                     GFGLGAHRGAQIILAKLGLNPDTDVTMLQIGGDPTRLAALLNGTIDASVFNPPLYQRA
                     VEAGMKVLANIEEMNFPVQGSALVTTDRFVKKNNDLVRRAVRSIVQSIHLIKTNPELT
                     KRAIKKYMRFKDDRDTEEAYQIMRDIAPRKPYPTVDGMKAVLDELSAKLSAAKTAQPR
                     DFMDTRFIEELDRSGFIDGLYK"
     gene            complement(3345..4400)
                     /locus_tag="VFN58_02710"
     CDS             complement(3345..4400)
                     /locus_tag="VFN58_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8916933.1"
                     /translation="MKGNAGNFTMPVLLLVFVAISLGFHLPLQAQTDAFFKGKTIRII
                     GGTGGFYESWARIIARHMGKHIPGNPNIIVQPMLGAGSLIAANYVYGVAKPDGLTVGM
                     FLNTIYLDQLVGRKEVQFSAEKFGWIGSPVGEAMMLYMRADTPYKSIADIIKVKEPPK
                     CGATGTASSSYILAMLLEETVGAKFISVLGYQGSGDTDMAVEKGEVVCRAHNISAHFG
                     REPFDSWHKKGFDRHIVQTGRKRDQNLPDTPTLYELMDQYKTPELSRRAVQVILAGGE
                     FGRPLVVTPGTPPERLNILRESFAKSMKDPELLAEAKKSKMDVDPSTAEELEALIKEA
                     MSQPKEVNERVRKLLGN"
     gene            complement(4853..5584)
                     /locus_tag="VFN58_02715"
     CDS             complement(4853..5584)
                     /locus_tag="VFN58_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916934.1"
                     /translation="MSQNLEQTVRQFAEDAKRIYGESLLSLILYGSAASSEYVEGRSN
                     INCLVLLKEVTPDELRKCAAQLPEWHKQGISTPLFVDPAYVRSSIDVFPVEFLDMKQR
                     YRVLYGQDFLQELEPKLERLRFQCEQELKGKMLRLRQLYLEASQTENRLVALLVKSTS
                     SFMVLFRALLHLQGTPAPQSMNEILVALSRLGLRTEAIERVHTLRREESALKPAEIDS
                     LFRQYLSEVQATVKFVEKTNAGQLS"
     gene            complement(5633..6580)
                     /locus_tag="VFN58_02720"
     CDS             complement(5633..6580)
                     /locus_tag="VFN58_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET8916935.1"
                     /translation="MTVLFRARFFLFELVFFLVLSINSSVPALDVPPLRARVNDLARI
                     LTPEKTQQLEEQLREFEQQTSHQITILTIPSLEGDPIEDFGIRVAEAWKIGQKGTANG
                     VILIVAQKERKIRIEVGRGFEGILPDVVASRIIREVIAPRYREGDYAGSIQAGIDSIL
                     EVTRSETVPKSSPTGPRRTLPGAGLFMLTALFALFVGISQRTPLRGALGGAISGGVIG
                     SPALWSSGFGILITLLIAGAVVGGLANFYTARAWGGPWTARRSRRESWPRDTVYYGGS
                     GDGGSGGDFGGGGFGGDFGDGGFSGGGGDFGGGGASGDG"
     gene            complement(6683..7669)
                     /locus_tag="VFN58_02725"
     CDS             complement(6683..7669)
                     /locus_tag="VFN58_02725"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916936.1"
                     /translation="MRRMILTILALLFLGKMTAGAGAQERLNLAYISPTAGSSSIFWV
                     AKDVGIFKKHGLDVNVIYIEGTPKALMSLFAGDLQVIAGTGPAVVSAKLRGGDVTMIM
                     GFEVFLPYYLMATPGINRIEDLKGKIGANHSAATSADFAIRLGLRGLGFDPDRDVTLR
                     VIGATNLRIIAMQQGQAHFTVIGNSERELAEKLGFKILADLASKRIPYPHSGVITSQR
                     MLREKHDTMLRFGRATIEGIHVFKTQKAPTIAVLKKYAKTDLTTLDNTYAYLKTALPD
                     APYPTLDGMKTLVAEVGRTQPTALRADPASFVDASILKALEDEGFLRRLSKY"
     gene            complement(7708..>7978)
                     /locus_tag="VFN58_02730"
     CDS             complement(7708..>7978)
                     /locus_tag="VFN58_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916937.1"
                     /translation="YTRAHTGILILASLLEHRVEVLADKGINEKVPAGTWNEVVQILT
                     VGLKSGKACDAYCKAIERCGDILAQHFPRPPDDQDELGNKLVIER"
BASE COUNT         1793 a         2210 c         2150 g         1825 t
ORIGIN      
        1 tcggacatct tctccaccta ccgtaatcat ctctcggatc tggaatttaa cccgatcatg
       61 ctgcgcgcgc ggggacaagg actcgcggcc gtcgatgtgc ggatgatccg gaagtaacct
      121 gtccatagag cgcttgcctg agactagaga gaattgattt tgttgagcta atgtccaacg
      181 acaggctacc cgctcatctt cgagcactga agatcggcgc acaatcgatg ggagcgctgg
      241 ccattggcgc gctagccatt ggtgcactga gtatcggcgc tttggccatc ggtcggctcg
      301 tgattggtcg ctcgcgaatt cgacgacttg agatcgatga gctcgtagtc gctcggctcc
      361 acgtcacggc ttccgtgcca agcacgccag cgactaagaa cgacccaccg acgaaagaaa
      421 caaaagagtc attcaaccaa cctgcgacct tcgctagagc actcaacaag ttgttcggat
      481 ttttcgttgg gctcggcttc ggtctaccgc ataactatct gcttcaagta cgcggtcgaa
      541 agagcgggcg agtctacgcg actccagtcg atgtgctcaa tcgagcgggc aagcgatatt
      601 tagtagcgcc gcgcgggcac acgcaatggg tacgtaacgc cctcgcaagc ggtatcgtat
      661 cactcaagaa aggacgacgg tctgaagagt tcaggatacg gatcctttca gacgacgaaa
      721 agccggaaat ccttaaggca tatctcgatc gttacaagct gacagtgcag cggtattttc
      781 cggtgcccgc cggctctgct accgacgagt ttcggccact gaccggaaat tatccggtct
      841 tcgagttgct accgatcccc taacctagaa tccgcggccc gaaaagtttc acggcattgt
      901 cccaaagtat cttgcgtttg gcgtcgtcgg aaatcggttg tgcgtcgagg tcgggaaggg
      961 ctcgcttggg atcgatggcg tccgggtgcg gatagtccgt gttccagacg atgttctcgc
     1021 ctttaaggtc atcgactacg tacttgagcg cgcgctcgtc gctgtcgcag gagatcatgc
     1081 actggcgcat gaagtagtcg ctcggacgca gccgcagctt ctccgacttg cccatgacgc
     1141 tgtttcgtcc atgggtgtga tcgtccatgc gtccgagcca aaaggggagc cagccggcgt
     1201 tggattcgag tatggcgagc cgcagtttgg gaaaacgctc aagaattccc gtaaaaataa
     1261 aatggccgag ggtggtcatg ttgtcgcaag gaaatcccag ggcatgatcc aggccgcgaa
     1321 gggcgagctg aagatcctcc cgccgttctt gttccagatg tctaaaggga tgaaaacggc
     1381 gctggcggta ttcgccgtga caggagatcg gaaagtcaag ctcgcaggcc gcctcccaga
     1441 gcgcgtcgta ctcgggctcg tgtaagtact tgccctcggg aagaaaggga ttgcgcacgg
     1501 agacagcccc aagttccacc accgcacggc gcgcttcctt caccatctcg ccgacgtccg
     1561 aacccggcag cacggccaca aacttcagcc gcttggaatc ggcgctgcag tagtcgcgac
     1621 accagttgtt gtaggcgcgg cacatcgcag cgcccagctc cacatccttc agcgcgacgc
     1681 gatacgcgaa gttgccgttg ttaccggtgg ggagcaggac ttgcatgtcc caaccatagc
     1741 gatccatatc ttttagacgg atatcagggg accaccactg gcggtaggct tcgccgtact
     1801 tttcttccag gaccgcccag gcgctgggcg gcggacggag ttggagatcg cctttgggaa
     1861 taatcttccc gtccggctcg taaaccatga aaacaccgtc catgaaagcg actttgggaa
     1921 cgcgatcgcg gaacttgggc tccacgtacc gctcccagag atactccggt tcgtgcatat
     1981 gcgcatcagc gtcaataacc ttcattccgt tccacatgat ggaaactcct ttgacgtccg
     2041 ttgattttga atcgacatta agtgaaacag acgcgaggtg tcaaggcgcg ttcattggaa
     2101 tgatcatgca aaatgatcta cagcgtggcg tctcttgtgc cgaggaaatg tttattggat
     2161 attcgcttag cacgcaaaga caaaagtgct gtgattttca ccggggaatg gaggaggatg
     2221 gcgatgcgcc aatgggtatt cgtgaagcct aaaaacttcg tgatattttt tccgctatca
     2281 tggctgttgc tatggacgtc ggctgcgcgc agcgcggacc agattcgggt cggctacggc
     2341 tcgttgagca caagttacgc ggctatttgg gtcgccggag aggcaggact gtttcgaaaa
     2401 aacggaatca acgcggaagt tctctacctc gagagcgcgc tcgtgcgcac ggcgctgatc
     2461 gccggcgata ttgccatggg cggcatgtcc ggaacgacga tggcggcgcc ccgcttgcag
     2521 ggcgccgatt ccgtcatcat cgcaagcttc gccaactcgc tgcagtaccg tttcgtggta
     2581 cggccggata ttcgcaccgt ggccgatctc aaaggcaagc gcgtgggcgt cgcgggcttt
     2641 ggtctcggag ctcatcgggg ggcgcagatc atattggcca agcttgggct aaatcccgat
     2701 acggatgtga ccatgctcca gataggcggc gaccccacac gtctggcggc cttgctgaac
     2761 ggcaccatcg atgcatcggt gttcaacccg ccgctatacc agcgggccgt ggaagccggc
     2821 atgaaagtcc tggcgaacat cgaagaaatg aactttccgg ttcaggggtc ggccttggta
     2881 accaccgatc gatttgtcaa gaagaataac gatctcgtgc ggcgcgcggt gagatcgatt
     2941 gtccagtcga ttcatctgat aaagacgaac ccggagctga ccaagcgcgc catcaagaaa
     3001 tacatgcggt tcaaggacga tcgggatacg gaggaagcgt atcaaatcat gcgggatatt
     3061 gcgccgagaa aaccttatcc caccgtggac ggtatgaagg ccgttctcga tgagcttagt
     3121 gccaagctgt cggcagcgaa aaccgctcaa ccgcgggatt tcatggatac gcgctttatc
     3181 gaagaactgg atcgctccgg atttatcgac gggctataca agtagaaggg gattttcgaa
     3241 cacgcggtcg catactctta agtaaggaca gaactaagtc gatgccgtaa aggtaacaat
     3301 tctacaacgc gtgtgagacg cagcgtccat acagatcgcg ctgattaatt tcccaaaagt
     3361 ttccttaccc gctcgtttac ctctttgggt tgactcatag cctcctttat cagggcctcg
     3421 agttcttcag cggttgaagg atccacatcc atcttacttt ttttagcctc ggccaaaagc
     3481 tccgggtcct tcatcgactt ggcaaaggac tcgcgcagga tgttcaagcg ctccggcgga
     3541 gtacccggag tcacgaccaa gggtcgaccg aattcaccgc ccgccaagat gacctgaacg
     3601 gcacggcggc tcaactcggg agttttgtac tgatccatga gctcataaag ggtgggcgta
     3661 tcgggtagat tttgatcacg tttgcgtcca gtctgaacga tgtgccgatc gaagcccttt
     3721 ttatgccagc tatcgaacgg ttctcggccg aaatgagcgg agatgttgtg ggcgcggcaa
     3781 actacttcgc ctttttccac tgccatgtcc gtgtccccag agccttgata gcccagcact
     3841 gaaatgaact tcgcgcccac agtctcctcc agcagcattg cgagtatata gcttgagctg
     3901 gcggtacccg tggctccgca cttcggcggt tctttgacct tgatgatatc ggcaatcgac
     3961 ttgtatggag tatccgcccg catatagagc atcatggctt ctccaaccgg cgagccgatc
     4021 caaccaaatt tctctgctga aaactgaact tctttgcgac cgaccaactg gtccagatag
     4081 atggtattta ggaacatccc caccgttaag ccatccggct ttgcgactcc ataaacgtag
     4141 tttgccgcaa tcaaggatcc cgcgccgagc atgggctgga caattatatt tggattgccg
     4201 gggatatgct ttcccatatg gcgggcgatg atacgggccc aactttcgta gaatcctccg
     4261 gtgcccccaa taatccgtat cgtctttcct ttaaaaaaag cgtccgtttg ggcctgaaga
     4321 ggaaggtgaa atcccagact aattgctacg aaaaccagga gcaaaaccgg catggtaaag
     4381 ttgcctgcat ttcctttcat agtggcctcc cctatcaagt cacatcgccg ccggctgggg
     4441 aaaagttccg cctatgccgt cgtttcacag ccagtaacga tcccatcact cgatctgtga
     4501 aggtttcatt aacccaagat ctcctggacg tgcaagtctg atcgcctttt ttaaggagag
     4561 taaaagaaaa caggaaagaa agaacaaact tctttgacaa aggatttttg ccgagcgccg
     4621 gtgcggcgca gtccctctag gaaaagcgtt ttacggtgct tgcaacccgc ttttatctag
     4681 gttcagagtc ggaatcacta agccggccgt tcgacttgct tgcaacgtcg atgaactatt
     4741 atcaagcctt tattccgtcc aaccatcacg ggatctcagt cctaaagtcc cctgacccgg
     4801 tgtgacgggt accggtggat attccgccga ccgctccgac agctgcagtt ggtcatgaaa
     4861 gttgcccggc gttcgttttc tctacgaatt taaccgttgc ttggacctcg gaaaggtatt
     4921 gtctaaagag tgaatctatc tcggcaggtt tcagagctga ttcctcgcgc ctcaaggtat
     4981 gaactctctc tatagcttcc gtacgaagac cgagtcgcga tagagcaacc agaatctcgt
     5041 tcatcgattg gggcgcgggg gttccttgca gatgcagtaa cgctcgaaaa agcaccatga
     5101 atgaactggt cgactttacg agcagcgcaa caagccggtt ctcggtttgc gatgcttcga
     5161 gatagagttg ccgcaatcgc agcatctttc ccttcaattc ctgctcgcac tgaaagcgca
     5221 agcgctccag cttaggttcc aattcctgca agaaatcttg cccgtaaagc actcggtatc
     5281 tctgtttcat gtccaagaat tcgactggaa agacgtcgat tgaggaccgc acataagcgg
     5341 gatcgacaaa gagaggcgtg ctgattcctt gtttgtgcca ttccggaagt tgagccgcac
     5401 acttccttag ctcgtctggc gtaacctcct ttagcaaaac gaggcaattg atattcgatc
     5461 taccctctac gtattcgctc gaagcggcgc tgccgtacag gatgagggag agtagactct
     5521 cgccataaat tcgcttggca tcttctgcaa actgtctcac tgtctgctcg agattctggc
     5581 tcatatattc acccttgggt ttcggctaca tgaaaccggt actttcaacg ctttacccat
     5641 cccctgatgc tcctccaccg ccaaaatccc cacccccgcc gctgaagcct ccatcaccaa
     5701 agtcgccgcc aaacccgcca ccgccgaaat cacctccact accaccatcc ccgcttccac
     5761 cataataaac cgtgtcgcgt ggccagcttt cccggcgtga acgtctggcg gtccacggtc
     5821 ctccccatgc tctcgctgtg tagaaatttg ccaaacctcc aacgactgcg cccgcaatca
     5881 atagggtaat taatattccg aaacctgacg accaaagagc agggctgccg atcacgccgc
     5941 cgcttatagc tccgccgagc gcgccgcgaa gtggagtgcg ttggctgatg ccgacaaaga
     6001 gagcgaataa agccgtcagc atgaacaagc ccgcgcccgg cagagttctt cgtggtccgg
     6061 ttggtgaaga tttcggtacc gtctcgctgc gggtgacttc caatatcgag tcaatgccgg
     6121 cctggatact gcctgcatag tctccctcac ggtaacgcgg agcgatcact tctcgaatga
     6181 tccgactggc gacaacatcc ggtaatatac cttcaaaacc acggccaact tcaatccgaa
     6241 tcttgcgctc cttctgcgcg acgatgagga tcacgccgtt agctgttcct ttttggccaa
     6301 ttttccacgc ctcggcaact cggatgccaa agtcttctat cgggtcaccc tcgaggctgg
     6361 gaatggtcag aatcgtgatt tgatgactgg tctgctgttc aaactcacgc aactgctctt
     6421 cgagttgctg tgttttctcc ggtgttagga tcctagcgag gtcgttgacc cgggcgcgca
     6481 gaggtgggac gtctaacgct gggacggatg agttaatcga caggacaaga aaaaaaacta
     6541 gctcaaacag aaaaaaccgt gctctaaaca ggactgtcat ctggttttag tcttacccat
     6601 gaccggttct ctggcgcgtc gttcgccgcg aagagcctcc gcgccgccgt cgatgtgcgg
     6661 gtcatccgca aaagaattca gtttagtact tgctaaggcg cctgagaaag ccttcgtctt
     6721 caagcgcctt gagaatgctc gcatccacga acgacgccgg gtcggccctc agcgcagtgg
     6781 gttgcgtccg cccaacctcc gcaaccaatg ttttcatacc atccaacgtg gggtaaggcg
     6841 cgtcgggaag cgcagtcttc agataagcgt acgtgttatc gagcgtggtg agatcggtct
     6901 tcgcgtattt cttcaaaaca gcgatggtgg gagctttctg ggttttaaag acatggatcc
     6961 cttcaattgt ggcacgaccg aaacgcagca tcgtgtcgtg cttctcccgg agcatgcgct
     7021 ggcttgtgat gacgccgcta tgagggtacg gaatgcgttt gctggcgagg tcggccaata
     7081 tcttgaatcc caacttttcc gcgagctctc gctccgagtt gccgatgacc gtgaagtgag
     7141 cttgtccctg ctgcatggca ataatgcgta gattagtggc gccgatgacc cgcagggtga
     7201 cgtcgcggtc cgggtcgaag ccgagaccgc gcaaacccag ccgtatggca aaatcggctg
     7261 acgtggcggc agagtggttc gcgccgattt tacctttaag gtcttcgata cgattaatcc
     7321 ccggcgttgc catcaagtaa tagggcagga aaacttcgaa tcccataatc atcgtgacgt
     7381 cgccgcctcg gagctttgcg ctgacaacgg ctggtccggt tcccgctatc acctggagat
     7441 cgccggcgaa gagcgacatg agcgccttcg gtgtcccctc gatataaatg acgttaacgt
     7501 cgaggccgtg tttcttaaat atgccaacgt ccttggccac ccagaaaatg gaagatgaac
     7561 ccgcggtcgg ggaaatatag gctaagttga ggcgttcctg agctcccgca cccgctgtca
     7621 ttttgcccag aaacagtagc gcgagaatag ttaggatcat tcgacgcatg ggatgcttct
     7681 tcctcggatt ttccacgagc ggtttcctta tcgctcaatg acaagcttgt taccgagctc
     7741 gtcctgatcg tccggcgggc ggggaaagtg ttgggccaga atatcgccgc agcgctcgat
     7801 ggctttgcag taggcatcgc acgccttgcc ggattttagg ccaacggtaa gaatctgaac
     7861 tacttcattc caggttccag ccggaacttt ctcgttgata cctttgtccg ctaggacttc
     7921 gactcggtgt tccagcagag aggcgagaat caatatcccg gtatgcgccc tggtgtag
//
LOCUS       DASSWZ010000108         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_272159, whole genome shotgun sequence.
ACCESSION   DASSWZ010000108 DASSWZ010000000
VERSION     DASSWZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272159"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="VFN58_02735"
     CDS             complement(<1..1961)
                     /locus_tag="VFN58_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916938.1"
                     /translation="MTKGARIATLVVLLALLGGGIYLAVRSRPPSAKLASNESTAKQS
                     PRLGAWVDEIVFREEADRAKALNMFEAGEIDVYGLGITDPELYRRIQASRVVQYEISY
                     GSSSELTFNPVGPTFPKTGELNPFHVPAIREAINWLVDRDYVAEEIYGGLAVPRYLPL
                     TTAFPDYARLADVARRLELHYSYDTEKAKAIIAKEMQKLGAQLVNQRWHYRSKPVRLI
                     FLIRTEDQRREIGDYVATLLENVGFTVDRQYKTAAESSPIWIGGNPADGRWHIYTGGW
                     VSLAIDRDQADMFNGYYTPRGRPDPLWQAYTPSPRLDKVAEILDRRDYSTWEEREKLM
                     TEALKLAMEDSVRVWLADVVYIWPRRKEVVLAADLAGGISGSTLWPYTIRFVEGTGRR
                     VNFANPSMLTEPWNPVAGSNWIFDNMITRGTQDLPAFPDPFTGLFWPQRIRTAEVYVQ
                     KGLPVIKTLDWLTLSFVPSIDVPPDAWIDWDAVGERFITVREMHPKGLKARTRTVVHY
                     GDNLWETRWHDGNKLALADFIFAFILGFDRANPKSEIFDESAVPALKTFLSHFRGARI
                     LEKNPLVVEIYSDQIFPDAESVAASRATDFYTNVPWHELALGVLAERNRQLAFSASKA
                     DRLRVQWLSYISGPSLAVLDRHLAAALKEG"
     gene            complement(1958..2800)
                     /locus_tag="VFN58_02740"
     CDS             complement(1958..2800)
                     /locus_tag="VFN58_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916939.1"
                     /translation="MTLRRLPTQGTRGKITRWVLLAVVTLAMVGCSTVARQIPSPPEL
                     DFGPPPVAGWTADKLAGILAQRDQQFQSLRSLASVHYRGPEGTQGFQEAVLVQRPDRA
                     RLETLTLLGAILIVTVNADQIAGFHPREGLYLRGKSSKENLFRYTRIPLELHEMTRLL
                     IGLPPVKNSADWQITGNSLYRQLNGQGKEIVVFDLAREIPIRWYRLGSDGSPELSAAF
                     DNFSSTPVGLFPSKITLEAAPQKRSLEIVYQEPEVNVEIAPSLFVQEKPASAQEVPIE
                     ALGR"
     gene            complement(2797..>3567)
                     /locus_tag="VFN58_02745"
     CDS             complement(2797..>3567)
                     /locus_tag="VFN58_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8916940.1"
                     /translation="DRAHYYLGATYAEMKSDDKALEEFLRVPEKSELFVDARIQAAYL
                     YDRRDQTQKAIETIQSALKKKNDQKEIFGLLASLYRKQKDYPKAIEAMERVIGLDAKN
                     DQAHFQLGALYDENKNKEKSIANMKKAIELNPQNAAALNYLGYTWAEMGVLLDEAEIL
                     IQRALKIEPNDGFYIDSLGWVYYQKGDYHQAVELLERAVEITVDDPTIIEHLADAYQR
                     VGKPDRALARYREALKKSKEDEQLKRIREKMQRLERKI"
BASE COUNT          753 a         1017 c          968 g          829 t
ORIGIN      
        1 ccctccttga gagcagccgc aaggtggcga tccaggacgg ccagactcgg cccgctgata
       61 tagctcagcc actgaactct aaggcggtcc gccttggagg cggaaaaagc caactgtcta
      121 tttctttccg ccaatacccc gagcgcgagc tcgtgccagg gaacgttggt ataaaagtcc
      181 gtcgctcggc tagcagcgac ggactccgca tccggaaaaa tctgatcgct gtatatctcc
      241 accaccagcg gatttttttc aaggatccgc gcacctcgaa agtggctcag aaaggttttt
      301 aaagccggca ccgcggactc gtcaaaaatt tcacttttcg ggttcgcccg atcgaaaccg
      361 aggatgaaag caaatatgaa atccgctaag gcaagcttgt tgccgtcgtg ccagcgagtc
      421 tcccagaggt tgtcgccgta gtggacaacg gtcctggtcc gcgctttcaa acctttcgga
      481 tgcatctctc gcacagtaat gaagcgctcg ccgaccgcgt cccagtcgat ccacgcatcc
      541 ggcggaacat cgatggaggg aacgaagctc aatgtgagcc aatcgagggt tttgattacg
      601 ggaagtccct tctgaacata gacttctgct gtcctgattc gctggggcca aaataacccg
      661 gtgaacgggt cggggaaagc aggtaagtcc tgggtaccgc gggtgatcat gttgtcgaag
      721 atccagttac ttcccgccac cggattccaa ggttccgtca gcatgctggg attggcgaaa
      781 ttgacccttc gccccgttcc ctccacaaat cggatcgtgt aaggccagag agtcgaacct
      841 gagatgcctc cggcaagatc ggccgccaaa accacctcct tgcggcgcgg ccatatgtat
      901 acgacgtccg ccagccagac ccgaaccgag tcttccatcg caagcttcag cgcttcggtc
      961 atcagttttt cgcgttcttc ccacgtgctg taatctcgcc ggtcgagaat ctcagcgacc
     1021 ttgtcgagcc ttgggctggg cgtgtatgcc tgccagagag ggtctggccg cccccggggc
     1081 gtatagtaac cgttgaacat gtccgcctga tcacggtcga tggctaacga gacccaacca
     1141 ccggtgtaga tatgccagcg gccatcggcc ggattaccgc cgatccagat cggagagctc
     1201 tccgcggcgg tcttgtattg acgatccact gtaaaaccta cgttctccaa aagcgtggca
     1261 acgtagtctc cgatctcccg ccgctgatcc tcggtgcgga tgagaaaaat gagcctcact
     1321 ggcttactgc gatagtgcca cctttggtta accaactggg caccaagctt ttgcatctcc
     1381 ttggcaatga tcgctttcgc cttttccgta tcgtaggagt aatggagttc cagccgccgg
     1441 gccacatcgg ctaagcgggc atagtcggga aaagcagtgg tcagcggcag gtatctcggc
     1501 acagcaagcc caccgtagat ttcctcggca acataatccc gatcgaccag ccagttgatc
     1561 gcctcgcgaa tcgcagggac gtgaaacgga ttaagctctc cggtcttggg aaaggtcggc
     1621 ccaacaggat tgaatgtcaa ttcggaactc gaaccgtagg atatctcgta ctggacaact
     1681 cgcgaagctt gtattctgcg gtagagttcg ggatccgtga tacccaatcc atagacatcg
     1741 atctctccgg cctcaaacat gtttaaggcc ttggcccgat cggcttcttc gcggaaaacg
     1801 atctcatcga cccaggcccc taaacgcggc gattgtttag cggtggattc gttagacgct
     1861 agctttgccg atgggggtcg ggaccgcacg gcgagataaa tcccgccacc cagcagagcc
     1921 agcaaaacca ccagcgtcgc aatgcgagct ccctttgtca tctccccagc gcctctatgg
     1981 gcacttcctg tgcgctagcc ggtttttctt ggacaaagag cgagggggca atttcgacgt
     2041 taacctccgg ctcttgatag acaatctcca aggatcgttt ttgcggcgca gcctctaagg
     2101 ttatcttcga aggaaacaga cccaccgggg tggaagaaaa attatcaaac gctgcgctca
     2161 gttcagggct tccgtcggat cctaaccggt accaccgtat cggaatttca cgagcgaggt
     2221 cgaacaccac tatttctttc ccctgcccat tgagttgccg gtaaagtgaa ttgccagtga
     2281 tttgccaatc ggcagaattc tttaccggcg gcaatccgat caggaggcgg gtcatttcgt
     2341 gaagctccaa gggaatccgg gtatagcgaa agagattttc cttagaactc ttgccccgaa
     2401 gatagagccc ttcccgcgga tggaatccgg cgatctgatc cgcattcacc gtcacgatga
     2461 ggatcgctcc caggagcgtc agcgtttcca gccgggctct gtcgggtctt tggacaagca
     2521 ccgcctcctg gaatccttgg gttccttcgg gcccgcggta gtgaaccgat gcaagggagc
     2581 gcagggactg aaactgttgg tctctctggg cgagaatccc cgctaatttg tcggcggtcc
     2641 agccagcaac aggcggtggt ccgaaatcaa gctctggggg tgacgggatc tgccgggcaa
     2701 cggtggagca gccaaccatc gccagtgtga cgactgctaa taaaacccat ctggttattt
     2761 ttccgcgggt gccttgcgtc ggcagccgac gcagggtcaa atttttctct ccagacgctg
     2821 cattttttcc cggatgcgtt taagctgctc gtcttctttc gattttttaa gcgcctcgcg
     2881 atagcgggcg agcgctcggt ccggtttgcc aaccctctgg taggcgtcgg cgagatgttc
     2941 gatgatggtg ggatcgtcca cggtgatctc caccgcccgc tccagaagct ccacggcctg
     3001 atggtaatca cctttctgat aatagaccca gcccaggcta tcgatataga agccgtcgtt
     3061 gggctcaatc ttcagcgcgc gttgaatcag aatttctgct tcatcgagca ggactcccat
     3121 ctcggcccag gtgtaaccga gatagttcaa cgcggcggcg ttctgggggt tgagctcgat
     3181 ggctttcttc atgttagcga tggatttctc tttgttctta ttttcgtcgt aaagcgcccc
     3241 tagctggaag tgcgcctggt cgttcttggc atccagccca atgacacgct ccatggcttc
     3301 gatcgccttg gggtagtcct tttgttttcg atagagggaa gcgagtaacc cgaagatttc
     3361 tttttgatca ttcttttttt tgagcgccga ctgaatcgtt tcgatagcct tttgggtttg
     3421 atcccgccgg tcgtagagat aagccgcctg gatgcgcgca tcgacgaaca actcgctctt
     3481 ctccggcacc cgcaggaatt cttctagagc tttgtcgtca gacttcattt ccgcgtaggt
     3541 agcgcccaaa tagtaatggg cccggtc
//
LOCUS       DASSWZ010000109        19017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1389925, whole genome shotgun sequence.
ACCESSION   DASSWZ010000109 DASSWZ010000000
VERSION     DASSWZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 19017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19017
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389925"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFN58_02750"
     CDS             <1..188
                     /locus_tag="VFN58_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916941.1"
                     /translation="MYAKEAEEASMKDVTTALLASLFVLITLVSLISADPVPHGWAAL
                     TSSGSLSELEFRYALRR"
     gene            complement(276..1298)
                     /locus_tag="VFN58_02755"
     CDS             complement(276..1298)
                     /locus_tag="VFN58_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET8916942.1"
                     /translation="MARDILVLVEHAEGKIESMTFQLLAIGRQLATEMKVELLAAAIG
                     HRMENILAALQGNGIDKILFVDDPALTLTGGEVQAHVFAEIARQIDPRLVLIGYSLVG
                     MELTPAVASKLGMNALTNCVNIELCDGAVTVTRPLFDGTMHAQIALEEHATAVVALQK
                     GSAQVTTPAAKQAVVQSITIEVNSLPSRSRVLEITEEPKSDVDLGKAEIVVAVGRGIG
                     DKEKIHFTAELAEALGGILACSRPVVDVGWLPRERQVGASGKSVAPKVYVACGISGAI
                     QHLTGMRDSKRIIAINKDPNAPIFQVAHIGVVGDLFEIVPALTRAAQEAKLAEKHPSG
                     ADLNTE"
     gene            complement(1301..2068)
                     /locus_tag="VFN58_02760"
     CDS             complement(1301..2068)
                     /locus_tag="VFN58_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET8916943.1"
                     /translation="MNIVVCCKAVPGLVTDLKIAADGKAIQYQGQLLAINECDEYALE
                     EALALRRNHGGQITAISMGNIKALDILYYALAKGADKAVRVDFESQDPRVASTAIAAA
                     LGKLDFDLVLTGTQSRDTLGGQVGIAAATLLNIPFAYAVMAVEARENNTVKVRKELGG
                     GRYAEVELTLPALLCVQTGIRPLTFVPPARRIRARQQPVVSFSLADIGLQSEQLAPRD
                     YRFVGIAAPERAHQVELLHGNPQEIASRLLTKIQESL"
     gene            complement(2081..3574)
                     /gene="tcuA"
                     /locus_tag="VFN58_02765"
     CDS             complement(2081..3574)
                     /gene="tcuA"
                     /locus_tag="VFN58_02765"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HET8916944.1"
                     /translation="MRTENCDVVVVGFGNAAQAAAVAAHNAGAKVLVLEKAPQAKKGG
                     NTWFSHGAQFRHTHNGVADEKPLLPHVSESEWEKIDLPPYTKDEFYADLMRVTRGRSV
                     PELAELLVNESYPTVKWMQEMGIQWEILYSAAKPEAGRFRWHHGSSFIHSKDGGGGLV
                     EMWHHIIDGKRIPVQFETAAARILTNESGRVYGVVVQSPEGFSEIHCKGLVLGSGGFS
                     ANPALRAQFLGSGWDLAKVRGTKYNTGDGIQMALDIGAQPFGHWSGGHATPIDADAGD
                     YEGGFLDPANRRYRTHRYAWTIGIMVNSEGRRFIDEGEDFHAYTYAKTGAEILKQPGG
                     IAYQIFDEKLKDAVGRYLYDGATPVIANSIKELAEMLEINSDALTKTVEEFNNAVVED
                     RPFNELIRDGRATKGLYPPKTNWAQKLDTPPYTAYAATCGLTFTYGGLKINTNCQVLN
                     KSDRPIPGLYAAGELTAGFFYYNYPSGSGLLRGAVTGKIAGTNAASQ"
     gene            3801..4187
                     /locus_tag="VFN58_02770"
     CDS             3801..4187
                     /locus_tag="VFN58_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8916945.1"
                     /translation="MVKTQGVVHFSLPVTDLERSAKFYAELLGMKIVQKTPRMVFLKC
                     GDDYFILAKDTPVKYDSLEQTPVHHAFKVAAEDFDPAVEFLKQNGVKIFHMEDRRDGV
                     FWGRQAYFLDPDGNKLEIHDPQGMGV"
     gene            4275..4631
                     /locus_tag="VFN58_02775"
     CDS             4275..4631
                     /locus_tag="VFN58_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8916946.1"
                     /translation="MHYQLEHVAVYCRDINESIRFYEKFFGGSPTAIRKGAAGYGFCF
                     MKIDGSPSIQLMESSGQVGVHHYGFVTDDIESVARDFNEKGAEIIRENRDQSGKLTTI
                     FLKDPNGLELEIRSPR"
     gene            complement(4658..5755)
                     /locus_tag="VFN58_02780"
     CDS             complement(4658..5755)
                     /locus_tag="VFN58_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8916947.1"
                     /translation="MMQSAEVRLDGIGKSYGEQWVVRGVNLHIRRGEFFTFLGPSGCG
                     KTTLLRIIAGFVFPDEGTVSLDGSPMNHVPPWQRNVGLVFQNYALWPHMTVFENVAFG
                     LRERKVARNEIEPRVMETLKQVDLQGTENRRPSQLSGGQQQRVALARTLVIQPRLLLL
                     DEPLSNLDAKLRIDMRIELLKLQHDLGLTTIYVTHDQEEALALSTRIAVINAGRVVQE
                     GTSRQIYEQPANEFVAAFVGQSNLFSGRLFQTQERFIEIQTDDNLIIRAASPPSATLP
                     HRGDKVLLSVRPEAMELRATTDSSQGANQLTGHVTASAYQGAFVEYEIQLAQRTLKTY
                     AVNPKGNALFQLGQEVAVMFTADDVVVVRSD"
     gene            complement(5758..7464)
                     /locus_tag="VFN58_02785"
     CDS             complement(5758..7464)
                     /locus_tag="VFN58_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8916948.1"
                     /translation="MAVSPVVIPLRTSERIDWWQGGLMAFLFAILTAFILYPVLSVMW
                     IALSDETGNLTVIHFLNFFRRPLFREALWNTLVSGLLVVVFSGLISLPLAYIIARYEF
                     RGKILLQTAATLPLVIPPFVGAVALQLILGRSGMVNLLLMRWFDTTVPFMEGLTGVVL
                     VQTLHFFPFILLNTVVSLSNIDPSMEEMAQNMGCHGFRLFRRITLPLMLPGFVAGSLL
                     TFIRAIDDLGTPLMLNYKNLLAPQAYLRITTVGMDDVDGYVVCVVLVVLSLVSLFAAR
                     KYLGLTEYATVQRSAPLRRHLQGKKLFLVWLLCGAILGISLLPHIGILLLSFSKVWSF
                     TLLPTTFTLGNFTEILFRAPHFIKNTLLYTLLAAGFDIILGAAIAFLLLRSRLAGRNL
                     LDAIATLPLAIPGVVLAVGYLRIFHGWDFPGLGAPLTSSWVILVIAYTMRRLPYTVRA
                     SYAALQQVHISLEESAQSLGANRLQTFAKITLPMITGGLVAGGLIGFVTSCVELSSTI
                     MLVPRIELGPISYGIYIYMQSPLGRGAGAALGVVAILLVSLGTYLTHRFFGGRAGNAF
                     RI"
     gene            complement(7522..8838)
                     /locus_tag="VFN58_02790"
     CDS             complement(7522..8838)
                     /locus_tag="VFN58_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8916949.1"
                     /translation="MKRISFWLVGTVFYFIASSALAKEVVIVTSFPKELFETYKKTFE
                     GKHPGVTVVINSKQTNAGVTYLRETKAKPEADIFWVSAPDAFQTLEAEGLLDKYAPPK
                     EIMARIPAKIGSFPIHDPDGKYFGFAISGYGLMWNKNYLQAHKLPAPKEWTDLTNARY
                     YGHLVISAPSRSGTTHLTVETILQAYGWEKGWALLLNSGGNMGTITERSFGVPEAVVS
                     GQYGIGVVIDFFGLSAIASGQPVEFAYPSLTSVVPASVGIVRGGPNPENARAFVNYLL
                     SEPGQMVLFSPEIARLPVIPDLYPKAPKDYPNPFKTKMGGVDFNDELSSGRRDVVNSL
                     YDHIITFRHRELRDAWGSIYKAEEALAKSKSSSATQGKTLLAEAKRLASQVPINDKRA
                     SDKEVAGAFKSKSGLKAQIETEWETFARSNYEKAKEQANKAAAMAR"
     gene            9103..10602
                     /locus_tag="VFN58_02795"
     CDS             9103..10602
                     /locus_tag="VFN58_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8916950.1"
                     /translation="MDLFNPHFFVVTLALVGLVIIIAALLSGLIDRSDLPQVGVFLAL
                     GAVLGPAGLDLLNVTLQSPIVAVVATLSLVLVLFTDAVSLSLAEIRRNGRLAFLVLGP
                     GTLLTAAMVALIGWLLLGLAPAAAVILGAALASTDPIMLRGLIRRPGLAEPVRLALRL
                     ESGLNDAVLLPIIFVAMPFLFHRGPPSGTEWARIGLEFFLLGPGAGIAVGLLGLSALD
                     MIRRKVGVRRDYESLYSLGIAFTAYAAAEAVHGSGFLAAFAAGLTIAALDVELCDCFL
                     EYGQTTAEMALLFAFVLFGSSLIWSGFTVLNLPILLFAIAVVLSRPIVFFLSLSGTRL
                     DFRSRLLIAWFGPRGLSSLLLILLPVFAGATGSQQLFEISCFVVILSVAVHGGSLMFL
                     KRDSFPVPARENVTVAANDLPQDLQASAKPLENENLEASTLRISVSELRRLQSSGAPI
                     LILDVRRDRNFEASEFQAQGALRISPDQPAQRIAELQIPRQTWLIAFCA"
     gene            10968..11972
                     /locus_tag="VFN58_02800"
     CDS             10968..11972
                     /locus_tag="VFN58_02800"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916951.1"
                     /translation="MKIRLVKAYCLLVIFLAIGFLSSSGIAADKLVGIHSARTMSQSM
                     PWIADEVGLFKKYDLEFQLVFISSAAMVTAAMLSGDGEVAITGGESITRAFTQGVTEL
                     IFIASAKNTLTHSILGKPEIKSPEDLKGKKLGLTRLGSNSHYFVIQALRKYGMGPTDL
                     NFIQTGGQVENLAALLSGAVDAATMTGPSGGARAMAQGFRYVVYGPDLRIPFGAATVV
                     ARRSSIRTRGPVIERFVRVMAEAVKIMFTDKELTYRVLAKQLRISDRKLLDAAYDEEI
                     KVMEPRLEFKMEAFQAILDETAKVDPRAKKIRPQDLIDRRYIDGLEKNGFFEKLWAAR
                     "
     gene            complement(12016..13620)
                     /locus_tag="VFN58_02805"
     CDS             complement(12016..13620)
                     /locus_tag="VFN58_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET8916952.1"
                     /translation="MTSRLAKFKWWQRGIIYQVYPRSFMDGDGDGVGDLAGIRSKLDY
                     LQGLGVDAIWISPIYPSPMADFGYDISNYTDIDPIFGTVDDFDALLADAHSRGLKVLL
                     DYVPNHTSDQHPWFLDSRSSRDSAKRDWYIWRDPKPGGAPPNNWLSNFGGGAWEWDEK
                     SGQYYYHAFLKEQPDLNWRNPEVQNAMLEVLRFWLDRGVDGFRVDVMHHMVKDNAFRD
                     NPPNPNWRPGMSPYRELLTTYTADLPEVQEIVAKFRSVVEEYDDRMLVGEIYLPVERL
                     MAYYGASGKGAHLPFNFQLIQLPWKAREIAAAVERYEALLPSYAWPNWVLGNHDKSRI
                     ATRVGPAQARVAAMLLLTLRGTPTMYYGDEIGMQDVPIPVEQVQDPFEKNVSGLGLGR
                     DPERTPMQWSGDEHAGFTRGTPWLPIADDYQTVNVSLERTRPTSILTLYRRLIELRRA
                     EPALSIGEFAPLPLDDELMAYIRKTDERRLLVVLNLGAEPKWFSISDLQCRGSLLLST
                     HLDRSREALQDKLALRSDEGAIIELL"
     gene            complement(13673..15295)
                     /locus_tag="VFN58_02810"
     CDS             complement(13673..15295)
                     /locus_tag="VFN58_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET8916953.1"
                     /translation="MLTRLFLILHLLLLNPLLAAELPDIDEQVRSIAQELRCVVCQNL
                     SVADSPSEMAQQMRGVVREQLRAGKTPQEVKDFFVSKYGEWVLLAPTTRGFSLLVWVL
                     PFVVLVLGIALALWLLRRWSANKTKPVGSPTNSDLLARVRSDATTSNALAIDPEDSSP
                     HAQLRQERARLYAELKELEFDYQAGKLAESDHAMLKLDIENKAAGVLQQLDSLPPRQT
                     SARQTTERQRSARKRSPNETNKRSYRGWQLATGGLFLLLFGLTLGIALTNSLRPREST
                     QDSITGGFLTGTGESGSTKFLQEGKSAFAKQDWPKAIEAFKQALAADPNQPEAHAYMG
                     FILVQAGHADGALLAFDKALSQAPNFPMALWGKGMVLLRDKQDYSGARQVLEKLAQLL
                     PAGAERSEVEKAIREMPAPEGKPRPQATAAAPSGQQIVGRISIDPKLKGNVDSQATLF
                     IIARADAAKGPPLAVKKIDRPVFPLSYSLGPENVMMQGMPFTGRVTITARLDKDGNPT
                     TRQPGDLTGDYQKNPVTVGSKNIDIVLDQVVQ"
     gene            complement(15300..15866)
                     /locus_tag="VFN58_02815"
     CDS             complement(15300..15866)
                     /locus_tag="VFN58_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET8916954.1"
                     /translation="MAWRRLLITVLLIAPVLWLLAFGFTRDARYITSPLLAKEAAPFT
                     VTLFDGRKISLNDLRGKAVFLNFWASWCTPCRAEAKDLEAAWQRLKDKNLVFLGVAIQ
                     DKEKDSRAFLQEFNITYANGWDESGKIAVDYGVWGIPESFFIDPQGRITYKHVGGIRA
                     ALVTVKLEEAARGIVSAKEGKEDYQPIR"
     gene            complement(15868..17829)
                     /locus_tag="VFN58_02820"
     CDS             complement(15868..17829)
                     /locus_tag="VFN58_02820"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET8916955.1"
                     /translation="MTATFGHTSILLALLFAAWGIFSPILGSRGNHQRYSAFTRTAIL
                     AQFVLVTLAAASLIYALVTTDFSIRYVAFNTTRATPIYYRVTGLWGALEGSLLLWEWI
                     LIIFSGLVAWIYRDRQRETMPWVLMVFSIVSAFFLGVIAFVSNPFETISPVPADGRGL
                     NPLLEDANMMTHPPLLYTGFVGLTVPYAFAMAALIRGKVDEAWIVATRRWTIIAWFFL
                     TAGNLVGAWWSYHVLGWGGYWAWDPVENAAFMPWLPATAFLHSVQVQERRRMLKVWNL
                     SLIIIAFSLTIFGTFLTRSGILSSIHAFSSGPVGGVFLVFLSFVLLSSFGLLAFRADL
                     LKGQPELDSMVSRESAFLLNNIVLVSALFTIFLGTIFPLLSEAIAGTQVSVGAPYFNS
                     VTVPLFLLLVFLMGVGPMIAWRRASWDNLKRNFLWPAAASLLAGVGLFIWKVRDFLPL
                     LAFTLLAFVVLTIFYDTALALRARHRNAGEGVIRGLVTLARRNQRRYGGLVVHLGVVL
                     VILGIAGSMTYSIEREATMSLKEQLNVGPYRVQFEGLTGTQQPTHFRVEGSFRVFHRG
                     DEWGVLSPALKFFPNQQSPVGRAVHRSTISEDLYLILSGFSELDKNQATLKVLVRPLV
                     VWMWIGGFVITLGTLLAILPLGKTSSEAA"
     gene            complement(17826..18272)
                     /gene="ccmE"
                     /locus_tag="VFN58_02825"
     CDS             complement(17826..18272)
                     /gene="ccmE"
                     /locus_tag="VFN58_02825"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET8916956.1"
                     /translation="MLKRRRFIIGGLIILAALSYIIYGGMQQAIVYFVTPSELKAKES
                     TSANKFLRMGGMVVTGSLQKDVKNLTYRFDLTDGNAAFPVFFRGVPPDLFTEGKGAVV
                     EGRIGPDGVFQATTIMAKHAEEYSPHLDGKEGYPKSFVPGKELSQQ"
     gene            complement(18414..>19017)
                     /gene="ccsA"
                     /locus_tag="VFN58_02830"
     CDS             complement(18414..>19017)
                     /gene="ccsA"
                     /locus_tag="VFN58_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET8916957.1"
                     /translation="PDAYQGEVQRIMYLHIPSILTAYLSFFLVFVGSCLYLWKREKRD
                     DILAYSAAEIGVLFTALTIIEGSIWGKPTWGVWWTWDARLTLTAILLLIFAGYLMLRS
                     LTEDENRAASAAAVLGIIGFLDIPLIHMSVYWWRTLHQPPSILRPDKAPWDNVHPALL
                     TALAINFVGFMLLYFYLLSQRFRLGEINEEIKARLLNRDN"
BASE COUNT         4305 a         5163 c         5363 g         4186 t
ORIGIN      
        1 ttatgtatgc caaggaagct gaggaggctt ctatgaagga tgtaacgacc gcactgctag
       61 ccagtctttt cgtactgata acattagttt ccctgatttc tgcagatcct gtaccgcacg
      121 gctgggcagc gcttactagc tcgggaagtc tctcagagct tgaatttcga tacgccttgc
      181 gaagataagc cggggttcgc tgtcttcacc tctgccagac aatatgagat tgccaccgga
      241 acgattttga agtcacagat tacaatatcc tccaattatt ctgtgtttag atccgcgcca
      301 gacggatgct tttcagcgag tttggcttct tgtgccgctc tggtcagagc cgggacaatt
      361 tcaaacagat cgccgaccac cccgatgtga gccacctgaa agatcggcgc attcgggtct
      421 ttattgatcg cgataatccg cttggaatct ctcataccgg tcaaatgctg aatggcgcct
      481 gagataccgc aggcaacgta gactttcggt gctacgctct ttccagaagc gcccacctgc
      541 cgctcgcggg gcagccatcc aacgtccacc acgggtcggg agcacgctag gattccgccg
      601 agagcctcgg ccagttccgc cgtgaagtgg attttctctt tgtcgccgat ccctcgtccg
      661 acagcaacga caatttctgc ctttccgaga tcgacatcac tcttgggctc ctcggtgatt
      721 tccagcacac gactgcgaga gggaagactg ttgacctcta tcgtgatcga ttgaacaacc
      781 gcttgtttgg ccgcgggtgt tgttacctgg gccgacccct tctgcagcgc taccacagcg
      841 gtggcatgtt cttccaaggc gatctgcgcg tgcatcgtac cgtcgaacag tggccgcgtc
      901 accgtcaccg caccgtcgca gagctctata ttcacgcagt tggtcaacgc gttcatcccc
      961 agtttacttg cgacggcggg agtcaactcc atcccgacga ggctgtagcc gatgagcact
     1021 aaccgggggt ctatttgcct tgcgatttcg gcgaagacat gagcttgcac ttccccgccg
     1081 gttaaagtta atgcaggatc gtcaacgaag aggatcttgt cgatcccatt accctgaagg
     1141 gccgctaaga tattctccat ccgatgtccg atcgccgcag ccagtagttc aactttcatt
     1201 tccgtcgcga gttgccggcc gatggccagc agctggaaag tcatggattc gattttgccc
     1261 tctgcatgtt ccaccaaaac aagtatatcg cgcgccatag ttagagactc tcctgaattt
     1321 tggtgagcaa tctcgatgct atttcttgcg ggttgccgtg aagcagctca acctgatgcg
     1381 cgcgttcggg cgcggcgata ccgacgaagc ggtagtccct aggggccaac tgttccgatt
     1441 gcaggccgat atccgccaat gaaaaagaaa ctacgggttg ctgccgagcc cgaatcctgc
     1501 gtgccggagg aacaaaggtg agaggtcgga tgccagtctg cacgcagagg agagcgggta
     1561 aagtcagttc cacctcggca taacgtcctc cgcccagttc tttgcggacc ttgacggtat
     1621 tgttctcacg cgcctcgact gccatcaccg cataggcaaa cggaatgttg agcagggtgg
     1681 cggcggcaat acccacttga cccccgaggg tgtcgcgcga ttgagttccc gtcagcacca
     1741 gatcgaagtc gagctttccc agagccgctg ctatggcggt actggcaacc cgcgggtcct
     1801 ggctttcgaa gtcgacgcgc acggccttat cagcaccttt cgccagcgca taatacaaaa
     1861 tatccagagc tttaatgttc cccatcgaaa tcgccgtgat ctggccaccg tggttgcgcc
     1921 tgagcgctag agcttcctct agagcatatt catcgcactc attgatggcg agaagctggc
     1981 cctgatattg gatcgctttg ccatcggcag cgatcttcag atcggttacc aagcctggta
     2041 cggctttgca gcagacgaca atattcattg tcgtgtacct ctattggctt gcggcgttgg
     2101 tgccggcaat ttttccggtt acggcgccac gcaaaagtcc cgatccgctc ggatagttat
     2161 aatagaaaaa tcccgcggtc agctcgcctg ccgcatacag tcccgggatc ggccggtcag
     2221 atttattcaa aacctgacag ttggtgttta tcttaagtcc gccgtaggta aacgtgagcc
     2281 cacaggttgc cgcataggct gtgtaaggcg gagtatcgag cttttgcgcc cagttggttt
     2341 tcggtgggta aagacccttg gtcgcccggc cgtcacgaat gagctcgttg aatgggcgat
     2401 cttctacgac ggcattattg aactcctcga cagtcttggt tagcgcgtcg gagttgatct
     2461 caagcatctc ggccaactct ttgatggagt tggcgatcac cggagtcgct ccatcgtaga
     2521 gatagcgacc gacggcgtcc tttagtttct catcgaatat ttggtatgcg atgccgccgg
     2581 gctgtttgag aatctcggcg ccggtcttgg cataagtgta ggcgtgaaag tcttcgccct
     2641 cgtcgataaa gcggcgtccc tcgctattga ccatgatgcc gatggtccag gcgtagcggt
     2701 gggtgcgata acgacggttc gcagggtcga gaaatccgcc ttcgtagtcg cccgcatcgg
     2761 catcaatagg cgtagcgtgg ccgccgctcc agtggccgaa cggttgcgcg ccgatatcca
     2821 gggccatctg aatgccgtcg ccggtgttgt acttggtgcc gcgcaccttg gccagatccc
     2881 agccgctgcc aaggaattgg gcgcgcagag ccgggttagc cgagaagccg ccgctgccca
     2941 gaaccaatcc tttgcagtgg atctcgctga atccttcggg gctttgcacg accacgccgt
     3001 aaacccgacc gctctcattg gtcaggatgc gagcggccgc ggtttcaaac tggaccggaa
     3061 ttcttttgcc gtcgataatg tgatgccaca tctcgaccag gccaccgccg ccgtctttgg
     3121 agtggataaa gctgctgccg tgatgccagc ggaagcgtcc cgcttcgggt tttgccgcgc
     3181 tgtagagaat ttcccactgg atacccattt cttgcatcca cttcacagtg gggtaggact
     3241 cgttaaccag caattccgcc agctccggca ccgatcgacc acgggttacg cgcatcaagt
     3301 cggcgtaaaa ctcgtctttg gtgtaaggag gaagatcgat tttttcccac tcgctttcgc
     3361 ttacgtgggg aagcagcggc ttttcatctg cgaccccgtt gtgagtgtga cggaactgcg
     3421 cgccatggct aaaccatgtg ttaccgccct ttttcgcttg cggagccttt tccagcacca
     3481 ggaccttggc cccggcgttg tgcgccgcaa cggcagcggc ctgagctgca ttgccgaatc
     3541 cgacgactac cacatcgcaa ttctcggttc tcatagatca ccctccggtt tttctggcac
     3601 tattttagat gccatatcat gacacatgga ctatgtcagt aactgagttg gaccgggctg
     3661 atgtcacaca gagcttgtaa gtaaagccaa agcgatataa ttcaaagatt gcgccaatgc
     3721 caagatgctt ggaggaacag atagctcaag accgagggtc gtagggaagt atcacctcca
     3781 gcaagcagaa ggaggcaatc atggtcaaga cccaaggtgt ggttcacttt agtcttccgg
     3841 tgacagacct ggagcgaagc gcgaagttct acgccgagct tctgggaatg aaaatcgtcc
     3901 agaaaactcc gcgaatggtc ttcctcaagt gcggcgacga ttattttata ttggcaaagg
     3961 acactccagt aaagtacgac tctctggaac aaacccctgt gcatcatgct ttcaaagttg
     4021 cggcggagga cttcgaccct gcagtggagt ttctcaaaca aaacggagtc aaaatattcc
     4081 acatggaaga tcgccgggac ggtgtcttct ggggcaggca ggcatatttc ctggacccgg
     4141 acggcaacaa gcttgagatt catgaccccc agggaatggg ggtttagata agagctgaat
     4201 aagcacgggg aactttgctt ggccgtgatg gtagctttag tacttgcgaa caaactagag
     4261 cggaggaaaa attaatgcac tatcaactcg aacatgtggc ggtttactgt cgagatatta
     4321 acgaatcgat taggttctat gaaaagtttt ttggaggtag cccgacagcg ataagaaagg
     4381 gcgcagcggg ctatggcttc tgctttatga aaatcgacgg ttcgccgtcg attcaattaa
     4441 tggaatcttc ggggcaggtt ggggtccacc attatggatt cgtcaccgac gacatcgaaa
     4501 gtgtcgcgag ggacttcaac gaaaaaggtg ccgagatcat tcgagaaaac cgcgaccagt
     4561 cgggcaagct cacaaccatt ttccttaaag atcctaatgg tttggagtta gaaattcgat
     4621 cccctcgtta gctgggactc aaaactgatc tcttgggtca gtcactgcga actacgacaa
     4681 catcgtctgc tgtaaacatc acggcgactt cttggccgag ttgaaagagt gcgttgcctt
     4741 tcggattgac cgcgtaggtt tttagggttc gctgcgccaa ttgaatctca tattccacga
     4801 aggctccctg gtacgctgaa gcagttacat ggcccgtaag ttgatttgcg ccttgcgagc
     4861 tgtcggtggt ggctctcagt tccatcgctt cgggacgaac gctaaggaga actttatctc
     4921 cccgatgtgg cagtgtggcc gatggcggcg atgcggcgcg gatgatcaag ttatcgtcgg
     4981 tctgaatctc tatgaatcgc tcctgcgttt ggaatagtct tccggaaaag agattggatt
     5041 gacccacaaa agcggcgaca aactcattgg cgggctgttc gtaaatttgc cgcgacgtac
     5101 cttcttggac gacacgtccc gcgtttatga cagcaattcg tgtcgaaagc gccagtgctt
     5161 cctcctgatc gtgggttacg tagatcgttg ttagcccgag gtcatgctgg agtttgagca
     5221 gctcaatgcg catgtcgatg cgcagcttgg cgtccagatt gctgagcggc tcgtcgagca
     5281 gcaggagcct aggctgaatc acgagggtgc gagccaaggc gacgcgctgt tgctgtccgc
     5341 cggagagttg tgaaggtcgg cgattttcgg ttccttggag atcgacctgt ttgagtgttt
     5401 ccatgactct tggttcaatt tcgttccgcg ccacttttcg ctcacgcaag ccaaaggcta
     5461 cgttctcgaa tacggtcatg tggggccaga gcgcgtagtt ttggaacacg aggccaacat
     5521 tccgttgcca gggcggcaca tggttcatcg ggcttccgtc aagagagacc gtaccttcgt
     5581 cagggaatac aaagccggcg atgatcctga gcagagtcgt cttgccgcaa ccgctgggtc
     5641 cgaggaaggt aaagaactca ccccggcgga tatggaggtt gacgccacga acgacccatt
     5701 gctcgccgta ggatttgccg atgccgtcaa gtctcacttc ggcggactgc atcatcgtta
     5761 gatcctgaag gcgttgcccg cgcgaccacc aaaaaagcgg tgcgttaagt aggttccgag
     5821 ggaaactaga agtatcgcaa cgacaccgag agcggccccc gcgccccgtc ccaacgggct
     5881 ctgcatgtaa atgtagatcc cgtaagagat gggccctaac tcgatgcgcg gcacgagcat
     5941 gatggtcgag gagagctcca cgcatgaggt cacgaatcca atgagcccgc ccgccaccag
     6001 tccgcctgta atcatcggaa gagtaatctt ggcaaacgtc tgcagacgat tggcgccgag
     6061 gctttgcgcg gattcctcga ggctaatatg aacttgctga agcgcagcgt aagaggcgcg
     6121 gaccgtgtaa ggcaaccgcc gcatggtgta ggcgatgacc aagatgaccc aagaagaggt
     6181 cagtggcgcg cccaggccgg gaaaatccca tccatgaaaa atccgcaggt aaccgaccgc
     6241 cagcacgact ccgggaatcg ccagcggcag agtagcgatg gcgtcgagca agttcctacc
     6301 cgctaatcgg ctgcgcaaca gcaaaaacgc aatcgcggca ccgagaataa tatcgaaacc
     6361 tgcggccagc aaagtgtaga gcagagtgtt ctttatgaag tggggtgctc tgaagagaat
     6421 ctccgtgaaa tttcccaagg tgaaagtcgt ggggagtagg gtaaagctcc acactttaga
     6481 aaacgatagc aacagaatcc ctatgtgcgg caacaggctg atgccgagta tggcgccgca
     6541 aagaagccaa acgagaaata gtttttttcc ttgaaggtgc cggcgtaagg gagcggaccg
     6601 ttgcaccgtc gcatactccg taaggccaag gtacttgcgg gcagcgaaca acgaaacgag
     6661 cgatagaact accagaacga cacagaccac gtagccgtcg acgtcgtcca ttcctacggt
     6721 ggtgatccgc aggtaagcct gtggcgccag gaggtttttg tagttcaaca tcaacggtgt
     6781 tccgaggtcg tcgatggcgc ggataaacgt caacaacgag ccggccacga aaccgggaag
     6841 catcagcggc agagtgatgc ggcgaaacag ccggaagccg tggcagccca tgttttgcgc
     6901 catctcttcc atcgagggat cgatgttaga aagcgagacc acggtattga gcaagataaa
     6961 gggaaaaaag tgtaaagttt gcacgagcac gacacctgtc aggccttcca tgaagggaac
     7021 cgtcgtgtcg aaccagcgca tcaataataa gttgaccatg ccgcttcgcc caaggattaa
     7081 ctgtaaggca acggccccga caaaaggcgg aatgactagt ggaagagtcg ctgcggtttg
     7141 aagcaggatt ttgccgcgaa attcgtagcg cgcgattatg taggcgagcg gaagtgaaat
     7201 aagcccgctg aagacaacca cgaggaggcc actcacaagg gtgttccaca aggcttcgcg
     7261 aaagagcggc cggcgaaaaa aattcaagaa gtggatcacg gtcagattgc cggtttcatc
     7321 cgatagagca atccacatca cgctcagcac tgggtaaagg atgaaagcag ttaggatcgc
     7381 aaagaggaac gccatgagtc ctccctgcca ccagtcgatt cgctcactgg ttcgaagcgg
     7441 tatgactaca ggggagaccg ccatggatgc atcgagtgta tgttagtgac cgtaagtttt
     7501 ggaagcgccg gaaccaggcg ctcagcgggc catagctgcg gccttattcg cttgctcttt
     7561 cgccttttcg taattgcttc tggcaaaggt ttcccattcc gtttcgattt gagcttttaa
     7621 accggacttc gacttaaaag ccccggcgac ctctttgtcg ctggcgcgct tatcattgat
     7681 aggtacttgc gaagccagcc ttttagcttc ggccaataaa gttttccctt gcgttgcact
     7741 gctactctta ctcttggcga gcgcttcttc ggctttgtaa atgcttcccc aggcgtctcg
     7801 taactcgcga tgtcgaaagg taatgatatg gtcgtataga gaattcacga cgtcgcgccg
     7861 gccagaggat agctcgtcgt tgaagtcgac tcctcccatc ttggttttga aggggttggg
     7921 ataatctttc ggcgcttttg gatataagtc aggaatcact ggaagtcggg caatctccgg
     7981 cgaaaagagt accatctgtc cgggctcgct aagcaaataa ttgacgaagg ctctggcatt
     8041 ttcgggattc ggtccgcctc taacgatgcc gacactggcg ggaacgacgg aagtcaagga
     8101 ggggtaggcg aactcaacgg gctgcccgct ggcaatagcc gagagaccga agaaatcaat
     8161 aactacgcca ataccgtact ggccgctgac gactgcttcc ggcaccccga aactccgttc
     8221 cgtaatcgtc cccatattgc cgccggagtt cagcaacaag gcccaacctt tttcccatcc
     8281 gtaggcctgg agaatggttt caactgtcaa gtgggtcgta ccgctgcgtg aaggcgcgct
     8341 gatgacgaga tggccgtagt agcgggcgtt cgtgaggtcg gtccactcct tcggcgccgg
     8401 caacttgtgc gcttgaagat agttcttatt ccacatcaag ccataaccgc tgatcgcaaa
     8461 accgaaatat ttcccgtcgg gatcgtgaat cggaaaactg ccgatctttg ccgggatacg
     8521 cgccataatc tctttgggcg gcgcgtattt atcgagtagt ccctcggctt cgagtgtttg
     8581 aaaagcgtcc ggggcgctca cccaaaagat atccgcttcc ggcttcgcct tggtctcgcg
     8641 cagataggta acaccggcat tggtttgctt gctgttaatg acgactgtga cacccggatg
     8701 tttcccttca aaagttttct tgtaggtctc aaacaactcc ttgggaaacg aggtgacgat
     8761 gacgacttct tttgccaacg ccgaactcgc aataaaataa aagacggtcc caaccaacca
     8821 gaagctgatc cgtttcataa aacctcctat cgctcgagaa tcaaaaaaca gcacagcggt
     8881 agcaactcac catgaagggt aagtcatgtc aagtcagaat agaagatcgc caaattctgg
     8941 ctgcgttgct accctaggct gccgtaagat gaagtcgaga aaaaggcaga gaaggcagcc
     9001 tttcgtacga gcctgacttg aaccctcagc ggacctgctg gtatcttcaa agccagcatc
     9061 gaagctcggc caagaccttc ccgactgctg ccaaaacaat gcatggatct ttttaatccc
     9121 catttcttcg tcgtaaccct ggctctcgtc ggccttgtta taatcatagc agcgctgctc
     9181 tcgggtctca tcgaccgcag tgatctgccg caggtaggcg tcttccttgc gcttggagcc
     9241 gttcttggcc cggcggggct ggatttgctc aacgtgactt tgcagtcgcc gatcgtcgcc
     9301 gtggtggcga ccttaagttt ggtcctggta ctgttcacgg atgcggtcag tcttagcctc
     9361 gctgagattc ggcgtaatgg gcgtctggct ttcctggttt tgggtccggg aacgcttctc
     9421 accgccgcga tggtcgccct cattggctgg ctactcctgg gtctcgctcc tgcggccgca
     9481 gttattttgg gcgccgcgct cgcctccacc gatccgatca tgttgcgcgg cctgattcgc
     9541 cgcccggggt tggccgaacc ggtccgactg gctctgcggc tagagagcgg tctgaacgat
     9601 gccgttctgt taccgatcat tttcgtggcg atgccgttcc tgtttcatcg cggccccccg
     9661 agtggcaccg aatgggcgcg catcggtctt gaattttttc tcttggggcc aggagcaggc
     9721 attgccgtcg ggctactcgg gctgtccgca cttgatatga tccggcgcaa agttggagtg
     9781 cggcgggatt acgaatccct ttactctcta gggatagcgt tcaccgccta cgctgcggcc
     9841 gaagcagttc acgggagcgg atttcttgcc gcctttgccg ccggcttgac gattgccgcg
     9901 ctcgacgttg agctttgcga ctgttttctt gaatatggtc agaccaccgc tgaaatggcc
     9961 ctgctgtttg cctttgttct gttcggtagt tcgctgatat ggagcgggtt tacagtgctg
    10021 aacctgccga ttcttctctt tgctatagct gtcgttctca gccgtcccat cgtttttttc
    10081 ctatcgctgt ccgggactcg gttggacttt cggagtcgtc tcttgatcgc ctggtttggt
    10141 cctcgtggat tgagctcgct gcttctcatc cttctgccgg tctttgcggg cgcaacggga
    10201 agccagcaat tgtttgagat ctcttgtttc gtcgtgatcc tttccgttgc cgttcatgga
    10261 ggctcattga tgttcctgaa gcgcgacagc tttcccgttc cagctcggga aaatgtcaca
    10321 gtcgccgcca acgacctccc acaggatctt caagcgtcag cgaagcctct cgaaaatgaa
    10381 aatctagagg catccacgtt gcgtatctcc gtgtccgagt tgcgccgact tcaatcatcc
    10441 ggagcgccga ttcttatcct ggatgtccgg cgcgaccgga attttgaagc cagcgaattt
    10501 caggcccagg gggcgctcag gatttcaccg gatcagccgg cgcaacggat tgccgaactg
    10561 cagatacccc ggcagacatg gttgatcgcc ttttgcgcct gacccgacga agcgacaagc
    10621 gcccgtgcgg cgctggagct acaaaaggcg gggtggggac gagcgcgcgc gctggtaggt
    10681 gggtgggaag catggcaggc agcgggacta ccgatggaag cgaaagcagg tctatgatct
    10741 agcgcggaat gcgccgccga tctaaacgcc tgggctctcg gcatttcagt ttgtgcaggt
    10801 tacgtgcaga cagacggtga cgtttgcagg gctggtttca ggtacgtgct tttggtatcg
    10861 actggcagca aggggcgctt tttgatagcg tccatcgacg cgatggttgt tttcttgcga
    10921 tgggtctcca gcctgagcct gggtagtttg ttcaggaggt tgtcctcatg aagatccggc
    10981 ttgtgaaagc gtattgcctg ctcgtcattt ttcttgctat cggttttttg tcttcctccg
    11041 gaatcgccgc agataaactc gtcggaattc actcagctcg tacgatgtcc cagtcgatgc
    11101 cgtggattgc cgatgaggtc gggctattca agaaatatga tctagaattc caacttgttt
    11161 ttatctcctc tgccgccatg gtgacggcgg cgatgctcag tggcgacggt gaagtcgcca
    11221 ttaccggagg ggaatcgatc acacgcgcgt ttacccaagg ggtcactgag ttgattttta
    11281 tagccagcgc caagaacacc ctgactcaca gtatcttggg aaagcctgaa atcaaaagtc
    11341 ccgaggatct caaaggcaag aagctgggtt tgacccggct ggggagcaac tcccattact
    11401 ttgttattca ggctttgaga aaatacggca tgggaccgac agatctgaat ttcatccaga
    11461 ccggcggcca ggtcgagaat cttgcggcat tactgagcgg cgccgtggat gccgccacga
    11521 tgaccggacc gtcaggcggc gctcgggcga tggctcaggg cttccgctac gtcgtgtatg
    11581 ggccggacct gcgtattccg tttggcgcgg caaccgtggt ggcacgccgc tcctcgatcc
    11641 gcacgcgcgg gccggtcatc gaaaggtttg tgcgcgtcat ggcggaagcg gtgaaaatca
    11701 tgttcacgga taaggaattg acttacagag ttttggctaa acagttgcgc ataagtgacc
    11761 gaaaattgct ggacgctgcc tacgacgagg aaatcaaggt gatggagccg aggttggaat
    11821 tcaagatgga agcctttcaa gcgatcctcg atgagaccgc aaaagtcgac ccgcgggcaa
    11881 aaaaaatcag gccgcaagat ctgatcgacc ggcgctacat tgacggactg gagaaaaacg
    11941 gtttttttga aaaactctgg gcggcaagat gatcgaaacc tttgccatcc aactgtctgc
    12001 cagattctga gaacctcaca acaactcgat aatcgctccc tcatctgagc gcaacgcgag
    12061 tttgtcttgt agcgcttctc ggctacgatc aaggtgggtc gaaagtaaca gggaaccgcg
    12121 gcattgcaag tcggatatac taaaccattt tggttcggcg ccaagattga gcacgaccag
    12181 cagcctacgc tcatccgtct tacgaatata agccatcagc tcatcgtcca acggcaacgg
    12241 ggcaaattct ccgatcgaca aagccggctc cgcgcggcgc agctcgatca agcgacggta
    12301 aagagtcagg atcgaagtcg ggcgggtgcg ctccagggag acgttgacag tttggtaatc
    12361 gtccgcgatc gggagccaag gcgtgcccct ggtgaagccg gcatgctcgt caccgctcca
    12421 ttgcatcggc gttcgctcag ggtcacggcc taaacccaga cccgacacgt tcttctcgaa
    12481 ggggtcttgc acttgctcga ctgggatggg cacgtcttgc atgccgattt cgtcgccgta
    12541 atacatcgtc ggcgtgccac gcaacgtgag caggagcatt gcggctacac gtgcctgcgc
    12601 tggacccacg cgtgtggcga tgcgggattt gtcgtgattg ccgagcaccc agttcggcca
    12661 cgcgtatgag ggcagcagcg cctcgtagcg ctccaccgca gccgcgattt cccgcgcctt
    12721 ccatggcagc tgaatcaatt gaaagttgaa cggcaggtgc gcgcccttgc cgctcgcgcc
    12781 gtagtatgcc atcagccgtt ccaccggcag atagatttcg cccaccagca tacgatcgtc
    12841 gtactcttcc acgacgctgc gaaattttgc gacgatctct tgcacctcgg gaaggtcggc
    12901 cgtgtacgtg gtcagcagct cgcggtaggg tgacattccc ggccgccagt ttggattggg
    12961 cgggttgtca cggaaagcat tgtccttaac catatgatgc atgacatcga cgcgaaaccc
    13021 gtccacgccc cggtcaagcc agaagcgtag cacctcgagc atcgcgtttt gtacttcggg
    13081 gttgcgccag ttgagatcgg gctgttcttt tagaaaggcg tggtagtaat actgcccgct
    13141 cttctcgtcc cattcccagg cgccgccgcc aaaattcgaa agccaattat tgggcggtgc
    13201 gccgcccggc ttaggatcgc gccagatgta ccaatcccgt ttagcgctgt cgcgcgacga
    13261 gcgcgaatcg aggaaccagg gatgttgatc cgacgtgtgg ttgggaacgt aatcgagcag
    13321 cactttcaag ccgcggctat gcgcgtcggc caggagcgca tcgaaatcat cgacggtgcc
    13381 gaagatcgga tcgatgtcgg tataattgct gatgtcgtag ccgaaatccg ccatcggcga
    13441 tggatagatc ggcgaaatcc agatggcgtc cacgccgagc ccttgcaaat agtcgagctt
    13501 gctccggatg ccggcgagat cgcccacgcc atcgccatcg ccatccataa acgaacgcgg
    13561 atagacttga taaatgatgc cgcgctgcca ccatttgaat ttcgccagcc gcgaagtcat
    13621 ttcaggtctg gacactgaat cacgccgtcg atacgcttcc ccatgccgct agttattgga
    13681 cgacctggtc gagcacgata tcgatattct ttgagcccac ggtcacagga tttttttggt
    13741 agtcgccggt taagtcgccc ggttgccgcg tggtagggtt cccatctttg tccaagcggg
    13801 ccgtaatcgt gacacggccg gtgaaaggca taccctgcat cattacgttt tcgggcccga
    13861 gggagtagga gagcggaaaa accggccgat cgatcttctt gacggccagt ggagggcctt
    13921 tggcggcgtc agcccgggca atgataaaga gagtagcttg gctatcgacg ttgcctttaa
    13981 gttttggatc gatagaaatc ctcccgacga tctgctggcc ggacggcgca gctgcagttg
    14041 cctgcggccg tggctttccc tccggggcag gcatctctct aattgctttt tctacctcgc
    14101 ttcgttccgc gcccgccgga agcagctgag ccaacttttc caatacctgc cgggcgcccg
    14161 agtaatcctg cttgtcgcgt aatagaacca tacccttgcc ccacaaggcc ataggaaaat
    14221 tcggcgcttg cgataaggct ttgtcaaacg ctagcaacgc tccatcggca tggcccgctt
    14281 gaactaaaat gaatcccata taggcgtgcg cttcaggttg gttgggatcc gcggcgagtg
    14341 cctgcttaaa ggcttcaatc gccttaggcc aatcttgttt agcaaaggcc gattttcctt
    14401 cctggagaaa tttcgtgctc ccactttcac cggtgccggt taagaagccg cccgtaatgg
    14461 aatcttgtgt cgactctctt ggacgcaaag agtttgtcag ggcgatgcct aacgtcaaac
    14521 cgaagaggag taagaagagc ccgcccgtcg caagctgcca tccgcggtaa gatcttttgt
    14581 ttgtttcgtt cgggcttctt tttcgcgcgg atcgctggcg ttcggtcgtc tgtctcgcgg
    14641 aagtctgtcg cggcggaagc gagtcgagtt gttgcaggac tccggccgct ttattctcga
    14701 tatcaagttt gagcatggcg tgatccgatt cagccagctt gcctgcctga tagtcgaatt
    14761 caagttcctt cagctcggca tagagccgcg cgcgttcctg gcggagctgg gcgtgagggc
    14821 tggaatcttc cgggtcgata gcgagagcgt tgctcgttgt cgcatcactc cggacgcggg
    14881 ccaggaggtc agagttggta ggactgccga cgggcttcgt tttgttcgcc gaccagcgac
    14941 gcaggagcca aagggccagg gcgatcccca gaaccaagac gacgaacggc aagacccaca
    15001 ccaaaaggct gaaacctctg gtcgtagggg cgagcaaaac ccattcaccg tatttggaga
    15061 caaaaaaatc tttgacctct tgcggggttt tgccggcccg cagttgctcg cggacgacgc
    15121 cgcgcatctg ctgggccatt tccgaaggcg aatccgcaac cgagagattt tggcagacga
    15181 cacagcgcag ttcttgcgcg atgctcctta cttgctcgtc gatatccggt agctcggccg
    15241 ccagtagcgg attcaggagg agcagatgaa ggatgagaaa cagtcgcgtt aacatgagat
    15301 caccgaattg gctggtagtc ttccttgcct tccttggcgc tgacaatacc gcgggcggct
    15361 tcttccagct tgacggtgac cagggcggcg cgaattccac cgacatgctt ataggtgatg
    15421 cgcccttggg gatcgatgaa gaagctttca ggaatgcccc agaccccgta gtccacggct
    15481 atctttccgg attcgtccca gccgttggca tacgtgatgt tgaattcttg aagaaaggca
    15541 cgggaatctt tctccttgtc ttggatggcg acgccgagaa agaccaggtt tttgtccttg
    15601 agtcgctgcc aagcggcttc gagatccttc gcctccgcgc gacagggggt gcaccaggag
    15661 gcccaaaaat tcaggaagac tgctttgcca cgcagatcgt tgaggctgat cttgcggcca
    15721 tcgaaaagag ttacagtgaa cggcgcagcc tctttggcca acaaaggcga tgtgatgtag
    15781 cgcgcgtcgc gcgtgaagcc gaaggcaagc agccagagga ctggcgcgat gagcaggact
    15841 gtaatcagga gtcgccgcca agccatttta cgctgcttcc gaggaagtct ttcccagcgg
    15901 caagatcgcc agcagggttc ccagggtgat gacaaatccg ccgatccaca tccaaacaac
    15961 gagagggcga accaacactt ttaatgtggc ttgattcttg tccagttcac tgaacccgga
    16021 caggatcaga taaagatcct cactgatcgt gcttcggtgc acggcccggc caaccggcga
    16081 ttgctggttc ggaaagaact tcaaggcggg agatagaaca ccccattcat cgccccgatg
    16141 aaaaactcta aaggaaccct cgactcggaa atgagtcggc tgctgcgtgc cggtaagacc
    16201 ctcgaattga acccggtacg gaccgacgtt caactgctct ttcaagctca tggttgcttc
    16261 acgctcgatg ctgtaagtca ttgagccggc aatccccaag ataaccaaca ccaccccaag
    16321 atgaacgacc aaaccgccgt agcggcgttg gttacgtctg gctaaggtta ccaagccgcg
    16381 aatcacccct tcaccggcat tgcggtgccg cgcccgcaac gccagtgcgg tatcataaaa
    16441 gattgtgaga accacgaaag cgagcaatgt aaacgccaac aacggcaaga agtcgcggac
    16501 cttccagata aacaagccga cgccggccaa cagcgaggcc gctgccggcc aaagaaaatt
    16561 ccgtttaagg ttgtcccagg aagcccggcg ccaggcaatc ataggcccca cacccataag
    16621 aaagaccaag agaagaaata aaggcaccgt gaccgagttg aaatagggcg cgccaacgct
    16681 gacctgcgtg ccggcaatcg cctccgagag caaaggaaaa attgtcccta ggaaaatcgt
    16741 aaaaagcgcc gacacgagaa cgatattgtt caacagaaaa gccgactccc ggctgaccat
    16801 cgaatccagt tcgggttgcc ctttgaggag atccgcccgg aacgccagga gaccgaagga
    16861 actgagcaaa acgaacgaaa gaaaaaccag aaagacacct ccgacaggcc ccgaggagaa
    16921 ggcgtgaatc gaagacagaa ttccagatcg cgtgagaaaa gttccgaaga tcgttaaact
    16981 gaaggcgatg ataatcaacg agaggttcca aaccttaagc atccgccggc gctcctgcac
    17041 ctgtaccgaa tggagaaagg cagtggccgg cagccagggc atgaaggcgg cattctccac
    17101 cggatcccat gcccagtaac cgccccaccc aagaacatga taggaccacc aagcacccac
    17161 gagattgccg gccgtcagaa agaaccaagc gatgatggtc cagcggcggg tggcgacaat
    17221 ccatgcctca tcgactttgc cgcgaatcaa ggcggccata gcgaaggcgt aggggaccgt
    17281 gagtccgacg aagccggtgt aaagaagcgg cggatgggtc atcatgttgg cgtcttcgag
    17341 caacggattg agaccgcggc cgtccgccgg tactggagag atcgtctcga aaggattgga
    17401 aacgaaggcg atgacgccaa gaaagaaagc ggagacaatg gaaaagacca tgagcaccca
    17461 aggcatcgtc tctcgttgac ggtcgcggta gatccaggcc accaatccgg agaagatgat
    17521 cagaatccat tcccagagca gcagcgagcc ttccaacgca ccccacaaac cggtgacgcg
    17581 atagtagatc ggtgtcgcgc gcgtggtatt gaaggcgaca taacggatcg agaaatccgt
    17641 ggtcacaagt gcgtagatca aggaagcggc cgccagcgtg accaatacga attgagccag
    17701 aatggcggtg cgggtgaaag ccgagtagcg ctggtgattg ccccgagagc cgagaatcgg
    17761 gctgaagata ccccaggccg cgaacaataa ggccaagagt atggacgtat ggccgaaagt
    17821 ggcggtcact gctggctcaa ctcttttcca gggacaaaac tcttcggata gccctcctta
    17881 ccatcgaggt ggggactgta ttcttccgcg tgcttggcca tgatggtcgt agcttgaaag
    17941 acaccatcgg ggccaattcg accctccacc actgcgcctt ttccttcggt gaagaggtcc
    18001 ggcggcacgc cacggaaaaa aaccgggaag gctgcgttgc cgtcagtcaa gtcaaaacga
    18061 taggtgagat ttttgacgtc tttttgcagc gacccggtga ccaccattcc gcccatacgc
    18121 aaaaacttgt tggcggaagt gctttccttg gccttcaatt ccgacggcgt aacgaaatag
    18181 acgatggcct gttgcatacc gccgtatatg atgtaggaga gggcggcgag aatgattaag
    18241 ccaccaatga taaaacgcct acgtttgagc attttttggt gactcctgcg gtgcgcacaa
    18301 ttgcgcgagt tctgtttctg cgcgccgaag ccggcgcttg agtgtaagcc agtagatcaa
    18361 gatcgcacac cacacaattc cataagccag gaaaacaaat ccccacgcgc ccatcaattg
    18421 tccctgttga gcaagcgcgc cttgatttct tcgtttattt cgccaagtcg gaaacgctgg
    18481 gagagcaagt aaaagtatag gagcataaag ccgacgaagt tgatcgcaag cgcggtcagc
    18541 agggccggat gcacgttatc ccagggcgcc ttgtccgggc gcaggatgga gggcggctga
    18601 tgcaaggtgc gccaccaata aaccgacatg tggatcaacg gaatgtcgag aaaaccgatg
    18661 atcccgagca cggcggcggc tgacgctgcg cgattttcat cctcggtgag cgagcgcagc
    18721 ataagataac cggcaaaaat caaaagcaag atcgccgtca gtgttaatcg tgcatcccag
    18781 gtccaccaaa ccccccaagt cggtttgccc caaatggaac cctcgatgat cgtcaacgca
    18841 gtgaacagga cgccgatctc ggcggcggaa taagctaaaa tatcatccct tttttctctt
    18901 ttccataaat agagacaact gccgacgaag acgaggaaga atgacagata ggcggtgaga
    18961 atactcggga tatgaaggta cataatgcgc tgcacttcgc cctggtaggc gtccggc
//
LOCUS       DASSWZ010000110         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_441786, whole genome shotgun sequence.
ACCESSION   DASSWZ010000110 DASSWZ010000000
VERSION     DASSWZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441786"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFN58_02835"
     CDS             <1..708
                     /locus_tag="VFN58_02835"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET8916958.1"
                     /translation="PNNAFIVMAGDFSTAEMLPKIKAVFGSIPRGSEPPKVRAEELPQ
                     QGERRVTLKKEAELPFVLMYYHTPNLKSPDSYALDVLSVVLAGGRSSRLYHDLVYQKH
                     LARSVDADYNGLSIDPSVFSVTAQVMPGKEPAEIEREIDSLLDQVKSELISDRELQKA
                     KNQIESAFVFGQDSIFGQAMKIGYYEAVGDWRLMDNYLDGIRKVTREDVQRVARQYLD
                     RDRRTVGVLIPTKENAS"
     gene            705..2024
                     /locus_tag="VFN58_02840"
     CDS             705..2024
                     /locus_tag="VFN58_02840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET8916959.1"
                     /translation="MKKSLGLFAILSICLLALPATANMVPQRSVLGNGMILLTSEQKA
                     LPMVSIELLIDAGSRHDMPKQEGLANLTARLLTYGTQRRTALQISDTLDFIGAGLSAG
                     CGEDLATVSMTVLKKDLATGLELLAEVLTLSTFPQEEIDRQKQSIVASIKAREENPGD
                     IAQRRFAAALYPQSPYGRPVEGSEASVKGLEQQSLRAFYERYYRPNRTILSVVGDISH
                     QEIARALNEAFRSWPKGEPATAPVAPSKIGTAETLRVNKELTQANIILGHEGVGRENP
                     DYYAIQVMNYILGGGGFSSRTLDSIRNERGLAYSVYSYFSAEKGHGTFELVMQTKNET
                     ALEAIRIAKAEIRRVREELVTEEELSDAKDYLTGSFPLRFDTNRKVANFLAQVEYFQL
                     GLDYPERYADFIKKVTREDVQHVAQKYLKPDTLITVIVANQKKIGDQ"
     gene            2237..2623
                     /locus_tag="VFN58_02845"
     CDS             2237..2623
                     /locus_tag="VFN58_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8916960.1"
                     /translation="MKKILIVEDHADMRELLTWQIELMGFMPIPAKHGKEGVEKAVAE
                     QPNLILLDIMMPGMDGWEAARTLRANPETKDIPILAATALFRDSDLKSCLDAGCNGYI
                     VKPFTFQELQGKVREFIASDNSALPS"
     gene            2690..>3589
                     /locus_tag="VFN58_02850"
     CDS             2690..>3589
                     /locus_tag="VFN58_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET8916961.1"
                     /translation="MSQKDKAIAIIKRLRDEGYESYLAGGCVRDMLLGKTPQDYDITT
                     NAKPDDIVKIFAKTVPVGAQFGVILVMVEGQPFEVASFRHDGPYLDGRRPSHVRYGSL
                     HEDIFRRDFTINGMVYDPIADQIIDRVGGRVDLDGKLIRAIGSPHSRFEEDRLRMIRA
                     VRFAASLDFAVESETFAAIRDLALTIKQISWERIGEEITRILTEGGAKTGFELLDRTG
                     LLQVLLPEISAMKGVEQSPDYHPEGDVFTHTMLTLSHLGSPTETLAYGCLLHDVGKPV
                     CIRRDEERLTFYGHTDKGAEMAEE"
BASE COUNT          850 a          921 c         1033 g          785 t
ORIGIN      
        1 cccaacaacg ccttcatcgt gatggccgga gatttttcca cggcagagat gctgccaaaa
       61 attaaggccg tgttcggaag tataccgcgc ggatccgaac ccccgaaggt gcgagccgaa
      121 gaactgcccc agcaaggcga acgcagggtt acattgaaga aagaagcgga gcttcctttt
      181 gtgttgatgt attaccacac acccaatcta aaaagcccgg acagctacgc gctggatgtg
      241 ctcagcgttg tcctggcggg gggccggagt tcgcggttgt accacgattt ggtttatcag
      301 aaacatttgg cgagaagcgt ggacgcagat tataacggct tatcgatcga tccctccgtc
      361 ttttccgtaa ccgcgcaagt catgccggga aaagagccgg cggaaatcga gcgcgagatc
      421 gatagtctct tggaccaagt gaagtcggaa ttgatcagcg accgcgaact ccagaaagcg
      481 aagaaccaga tagaatccgc ctttgtcttt ggccaggact cgattttcgg acaagcgatg
      541 aagattggtt actacgaagc ggtgggcgac tggcggctga tggacaatta tctggacggc
      601 atccgtaagg tgactcgcga ggatgttcag cgtgtcgcaa gacagtactt ggaccgtgat
      661 cgtcgaaccg tcggcgtgct gattcccacc aaagaaaacg cctcatgaaa aaatctctag
      721 gtctcttcgc tatcctctcg atttgtttgc tggcgctgcc ggcgacggca aacatggtcc
      781 cgcagcggtc ggttctggga aacgggatga tccttctaac gtccgagcaa aaggcgctgc
      841 cgatggtttc catcgagctt ttgatcgatg ccggctcccg ccacgacatg ccgaaacagg
      901 agggcctggc caacttaact gccaggctct tgacctacgg gacgcaacgc cgaacggcgc
      961 tgcaaatcag cgataccttg gattttatcg gcgccgggct ttccgcgggt tgcggtgagg
     1021 atctcgccac tgtgagcatg accgtgctca agaaagattt ggccacaggg ctggaactcc
     1081 tggctgaagt tttgaccctg tcgacttttc cgcaagagga gatcgatcgc cagaagcaat
     1141 cgattgttgc atccatcaaa gcacgagaag agaatcccgg ggatatcgcc cagaggagat
     1201 tcgcggcggc gctttatccg cagagcccgt acgggcggcc ggtcgaagga agtgaagcat
     1261 cggtaaaggg actggaacag caaagcctga gagccttcta cgagcgctat tatcgaccca
     1321 atcggactat tttgtcggtg gtgggcgata tctcgcatca agagattgcg cgggcattga
     1381 acgaagcctt ccgttcctgg cctaaaggcg aaccggctac ggcgcctgtc gctccttcga
     1441 agatcggcac agcagagacc ctacgggtca ataaagagct cacccaggcg aacatcattc
     1501 tcggccatga aggggttggt cgggaaaacc cggactatta cgccattcaa gtcatgaatt
     1561 atatattggg cggcggcggg ttttcctcga ggactctgga ctcgatccgc aacgagcgcg
     1621 ggttggctta ctcggtttac agctacttca gcgctgaaaa gggccacggc acctttgagc
     1681 tggtcatgca gacgaaaaat gaaaccgcgc tggaagccat ccgtatcgcc aaagcagaaa
     1741 ttcgtcgcgt gcgcgaagag ctcgtcactg aggaagaact ttcagatgcg aaggattatc
     1801 tgaccgggag ctttccgtta cgcttcgata ccaaccgcaa agtcgcgaat ttcctggccc
     1861 aggtcgaata tttccaattg gggttggact atcccgaacg atacgccgat tttatcaaaa
     1921 aggtcacacg tgaagatgta cagcacgtgg cgcagaaata tcttaagcct gacacgctca
     1981 tcacggtaat cgtcgcgaat cagaagaaga ttggcgacca gtgagagttt atcgttccct
     2041 gcaccttagt ttcactctat tccatgtttc agcccgcacg ttattgcaat gtgcggtgct
     2101 ttgcagcgca tttacacaac ggccggcaag ggctgttgac agctagacaa ggggtgcggc
     2161 atagtttggg atgagttgag tgcccaggct ctctcagtag tgcagtgact ccatgcagcc
     2221 ggagacaacg gatttgatga agaaaatcct cattgtcgaa gatcacgcag atatgcgcga
     2281 gctgctgact tggcagatcg agcttatggg atttatgccg atccccgcga agcacggcaa
     2341 agaaggtgtg gagaaagccg tcgccgagca gcctaacctc attctcttgg atatcatgat
     2401 gccgggaatg gatggttggg aggctgcacg aacgctgcgg gcgaatccgg aaactaaaga
     2461 tattccgatc ttggctgcaa ctgccttgtt ccgcgattct gacctgaaaa gttgtctcga
     2521 cgccggatgc aacggataca tcgtcaagcc ctttacgttt caggagctgc agggaaaagt
     2581 gcgggagttt attgcttcgg ataacagcgc gttgccatca tagtttttcg ccctgatctc
     2641 ctttcattgc cgatcgttct ctactcggat aaccgcgaga agtttgtcga tgtctcaaaa
     2701 agacaaggcc atcgccatta ttaagcgtct gcgcgacgag ggctacgaaa gttacctggc
     2761 gggcgggtgt gtgcgcgata tgctgctcgg aaaaacgccg caggattatg atatcacgac
     2821 gaacgccaag cccgacgaca tcgtcaagat tttcgccaaa accgttccgg tgggcgcgca
     2881 gtttggcgtc atactggtca tggtcgaagg ccagccattc gaggttgcaa gttttcgtca
     2941 cgatggcccc tatctggacg gccggcggcc gagccacgtg cgctacggca gtctgcacga
     3001 agatattttt cggcgcgact ttacgatcaa tggaatggtc tatgatccga tcgcagatca
     3061 aattatcgat cgcgtcggcg ggcgagtgga tctagacggc aagttgatcc gcgccatcgg
     3121 tagtcctcat tctcgttttg aagaggaccg gctgcgcatg atacgagcgg tgcgttttgc
     3181 cgccagcctg gattttgcgg ttgaaagcga aacgttcgcc gccattcgag acctggcgct
     3241 caccatcaaa caaatttcct gggaacgcat cggcgaggag attacgcgta ttttgaccga
     3301 aggcggggca aaaaccggat ttgagttgtt ggatcggacg ggtctgctgc aggttttgct
     3361 acccgaaatc agcgccatga aaggggtcga gcaaagtccg gactatcatc ccgaaggtga
     3421 cgttttcacg cacacgatgt taacgctcag ccacctgggt tcacctacag agacattggc
     3481 ctacggttgc ctgctgcacg acgtgggtaa gccggtttgc atccggcgcg atgaggagcg
     3541 gctcaccttt tacggccata cggacaaggg ggctgaaatg gctgaggag
//
LOCUS       DASSWZ010000111         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_314750, whole genome shotgun sequence.
ACCESSION   DASSWZ010000111 DASSWZ010000000
VERSION     DASSWZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314750"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFN58_02855"
     CDS             <1..1354
                     /locus_tag="VFN58_02855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET8916962.1"
                     /translation="SSCSSSSQVAKDILPSISGPSEDEEVRISREFRREARKQLQFVT
                     NPEVERYIDRIGQRILSTMGPQPFDYRFFVIQDPQLNAFAVPGGSIFFFTGLLEKTKT
                     TSEVAGVLGHEITHIKGHHMARQSGPDAISLLSMLGALLLARTGSGGQAAGTLGQAIA
                     ATRQIAYSRQLEMEADTLGVRYMSAAGYDPKGSLGFLKTLDQERALNPIDVPAYMMTH
                     PITQERIANTELVIRSLGKTEIKVEEPDLLKKMQIIIRIERRDSDAVLAEYDKLVRQN
                     PESAESLHLLGFAQQLTGQLTQAKQNYEKARRLIPENPDLNRDIGRLYTQTGDFASAR
                     VAFNQALALDPKESLTYLYLGELHEKEGDLRGAAGAYLNAYNLSPFWDKPPYRLSVVY
                     GKLNRLGDAYYYHGRSLLLQDDDLRAAADFERAIKVLGDSPRAQLIKEELAALRARRR
                     "
     gene            complement(1325..1732)
                     /locus_tag="VFN58_02860"
     CDS             complement(1325..1732)
                     /locus_tag="VFN58_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916963.1"
                     /translation="MYDDFLARILEPDIFLPSQFYGNGGLSRQLEGEKRLMIAILKDA
                     VECLDKYRGARNSSGRSHYESALEWVDDKGTEWLFSFTNICDFLGFDPDYLRQVLLKR
                     ENQYVKPVSTRVYTFPALSKREIGLPSSRAESR"
     gene            complement(2021..2731)
                     /locus_tag="VFN58_02865"
     CDS             complement(2021..2731)
                     /locus_tag="VFN58_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8916964.1"
                     /translation="MSELNDLKKKLAMSSRMLFNSGLVDYSGHISARIPGSDQLLILP
                     HPVSRAIVVADDMVVTDFEGKLIEGKYKAPSEVYMHARAYKARGDIQSVAHLHNHMVA
                     VLTMVDKPFYPASSNPGAFFGSGPMPVYMDPALIHSVEQGDAVARTLGKGDAVMLRGH
                     GSMVVGQAIEWVFAACVDLEEAASRFYKASLLGPVRVYSEDETQRTIKARRKDSVVQK
                     IWDHNVAKTQLAGLMEGV"
     gene            2877..>3440
                     /locus_tag="VFN58_02870"
     CDS             2877..>3440
                     /locus_tag="VFN58_02870"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase"
                     /protein_id="HET8916965.1"
                     /translation="MSTSSADGIRWDLNDLFSSHDDPRIETTLTDCHARAEAFATRFR
                     PVMEHSETLTPDVLLDAIKELEIIYEALGRVGSYAGLLYASDTANPDYQNLEQRVEQR
                     STEIRNLLLFFEIQWLKAEDPIVDRLIGDPPLKTYNHYLQSLRRFRPHTLSEPEEKII
                     NEKDNTGRNAFGRLFSEVTSSLTFVLEK"
BASE COUNT          833 a          984 c          859 g          764 t
ORIGIN      
        1 aagcagctgc agctccagct cgcaggttgc aaaagacatt cttccttcca taagtggccc
       61 cagcgaagac gaagaagtta gaatcagccg cgaatttcgg cgcgaagcgc gaaagcaact
      121 ccaattcgtc accaacccgg aggttgagcg ctatatcgac cggattgggc agcgtatatt
      181 gtccaccatg ggaccgcagc ccttcgatta tcgttttttt gtcatccaag acccccagct
      241 caatgccttt gcggttcctg gaggatcgat tttctttttc accgggctgc tcgaaaagac
      301 caaaacgact tccgaggtcg ccggggttct aggtcatgaa atcacccaca tcaaggggca
      361 ccacatggcc cgccaatcgg gacctgatgc catttcgctt ctcagcatgc tcggagccct
      421 cctattggcc cgcaccggct cgggaggaca ggctgctggg accctcggtc aggccatcgc
      481 ggcgactcga cagatcgcct acagccgtca attggaaatg gaggctgaca ccctgggagt
      541 gcgctacatg tcagcggcag gatatgaccc caagggctcg ctcggttttc tcaagaccct
      601 cgaccaagag cgcgcgctaa atcctatcga tgtccctgcg tacatgatga cccacccgat
      661 tacccaagag cgcatcgcca acaccgagtt ggtaatacgc tcgctgggaa aaaccgaaat
      721 aaaagtcgaa gagcccgatt tgctaaagaa aatgcagatc attatacgta tcgagcggcg
      781 tgattctgac gcggtcctgg cggaatacga caagctggtc cgtcagaacc cggaaagtgc
      841 cgagtcgctc cacttgctcg gcttcgccca acagctcacg ggacaattga cgcaggcaaa
      901 gcaaaactat gagaaagcgc gacgattgat tccggaaaat ccggacctga accgcgatat
      961 cggtcgtttg tatacgcaaa ccggggactt tgcatccgcc cgtgttgctt tcaatcaagc
     1021 cttggcatta gacccgaaag aatcgctcac ctatctttac cttggggagc tccatgaaaa
     1081 ggaaggggat cttcgtggtg cagccggcgc gtacttgaac gcttacaatc tctctccgtt
     1141 ttgggataag ccgccgtatc gcctaagcgt cgtttatgga aaattaaacc ggctcggaga
     1201 tgcttattac tatcacggcc gctcgttgct gctccaagac gacgatctgc gggcggctgc
     1261 cgattttgaa agggcgatca aagttcttgg cgactctccg cgcgctcagc tcattaaaga
     1321 ggaactagcg gctctccgcg cgagaagacg gtaaccctat ttcccgctta cttaaagcag
     1381 gaaaagtata gaccctcgtg ctgacgggct tcacatattg attttcccgc ttcaataaca
     1441 cttgacgcag atagtcaggg tcaaatccca agaaatcgca gatgttagta aaagaaaaca
     1501 gccactctgt gcccttgtca tccacccatt cgagcgcact ctcataatgg cttcggcccg
     1561 agctgttgcg agcacctcta tacttatcaa ggcactcaac ggcgtccttc aggatcgcta
     1621 tcatcagtcg cttttccccc tctagctggc gcgataatcc gccattgcca taaaattgag
     1681 agggcagaaa aatatccggc tcaagaatcc ttgcgagaaa atcatcatac atgtggcgat
     1741 gctcctagat tagttgaacc gcattctata acacggggca tacctcgatg caactagaaa
     1801 tcgccgcaca gctcggatca acacacaatc actgagagta agaataacct taggaaatct
     1861 aagcattagg gaaagtcgtg tcacccccgg aaaccctgcc ggtggctaag ggtaaacgga
     1921 aaccgagcgg ccaaaccgca tgcgttcgac tcaccttcag gcacgttagt ctttgcccag
     1981 tgcgtgcggt cgtgcggttc aacgtcgccg gatttgcccg ctacactccc tccatcaatc
     2041 ctgccaattg ggtcttggcg acgttgtggt cccagatttt ctgaactacg gagtccttac
     2101 gccgggcttt gatggtgcgc tgcgtttcgt cctcgctata gacccggacc ggaccgagta
     2161 aggacgcttt gtaaaacctc gacgccgcct cctcgagatc gacacaggct gcaaagaccc
     2221 actcgatcgc ttggccgacg accatcgagc cgtgacctcg cagcatgacc gcgtctccct
     2281 ttcccaaggt ccgtgccaca gcatcgccct gctcgacgga gtgaatcagg gcggggtcca
     2341 tgtaaaccgg catcgggccg gaaccgaaga aagcccccgg attagatgaa gccggataaa
     2401 acggcttatc caccatggta agaaccgcca ccatgtgatt gtgaagatgg gcaacactct
     2461 ggatatcgcc gcgcgccttg tacgcgcgcg catgcatata gacttccgag ggtgccttat
     2521 atttgccttc gattagtttt ccttcgaaat cggtaacgac catatcgtct gccaccacga
     2581 ttgcacggct taccggatgg ggcagaatca aaagctgatc cgatccggga attcgcgcgc
     2641 tgatatggcc gctgtagtcc accagccctg aattgaataa catccgcgat gacatggcca
     2701 acttcttctt aagatcgttg agttctgaca cggttgatct cccttcgtgg ctaattgttt
     2761 ttaaatgaat tcgagcatca tacacgctga atccaacaag tcgcaactgt tagccatcgg
     2821 cccttgctcg gtgcccagag agatggttta tacaaaagag aatacctgga ggactcatgt
     2881 cgactagtag cgccgacggc attcgctggg acctgaacga tctcttttcc tcacatgacg
     2941 acccccggat cgagacaacc ttgaccgact gtcacgctcg agctgaagcc tttgcgactc
     3001 gatttcgccc cgtgatggag cattcggaaa ctctcacgcc tgatgtcctg ctcgatgcaa
     3061 tcaaggagtt ggagatcatc tacgaagcct tgggacgtgt cggcagctat gccggattgc
     3121 tttacgcttc ggacacggcc aatcctgact atcagaactt ggagcaacgc gtcgagcaac
     3181 gctcgacgga gattcgtaac ctgctgctct tttttgaaat ccagtggctc aaggccgagg
     3241 atccgatagt cgatcgattg atcggcgatc ctcccctgaa aacttacaac cattatcttc
     3301 aaagcttgcg ccgctttcgc ccgcacacgc tttccgagcc ggaagagaag atcatcaatg
     3361 aaaaagacaa taccggccgc aacgccttcg ggcggctttt ttcggaagtt acctcgtctc
     3421 tgacttttgt gctggaaaag
//
LOCUS       DASSWZ010000112         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_88354, whole genome shotgun sequence.
ACCESSION   DASSWZ010000112 DASSWZ010000000
VERSION     DASSWZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88354"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFN58_02875"
     CDS             <1..293
                     /locus_tag="VFN58_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET8916966.1"
                     /translation="GPWITTKDEVSDPHNLWMKLWINGEVKQSAKTDAMINDIATQVS
                     FLSQVTTLYPGDLITTGNPDSPEFQEKLKPGDVLKAEIEGIGAMSLGVAKES"
     gene            453..1787
                     /locus_tag="VFN58_02880"
     CDS             453..1787
                     /locus_tag="VFN58_02880"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET8916967.1"
                     /translation="MVRIAPFRGLFYNHKKIRDLSKVIAPPYDVISKEEQDKLYRKSP
                     YNFVRLDLNQEPDSYSAVAQLFQEWQAEGIFERDEVPAIYYSTHRFSVRGGEERERQG
                     FFALTELQDFSGGDIRPHEKTLDAPKEDRLKLMLACHAQLSPIFALYAEPKSSVNRML
                     AAGIEGIDPFIEVKQDGTGECRLWRVTDPELVRRVQQEMRDQPLLIADGHHRYEATLK
                     YRDQVRREQSQWNGREAFNYIMAYFANMDDENLVILPTHRLVRGYAPQPFLQLEDTLQ
                     KYFYVEQYPKTPEGKGWFLKALKSGAKKQRLIGASFKRDPRYLILRLKNKRIMQRLAK
                     DLSEPLRELDVTTLHLLILEHILGLAPDQQVSGETIRYSQDEEAMLQALEKEDFQAAF
                     ILNPPKAEEILTIANGGEKMPQKSTYFYPKLISGLIVNKIDPQEEIEDPPAK"
     gene            complement(1818..>2250)
                     /locus_tag="VFN58_02885"
     CDS             complement(1818..>2250)
                     /locus_tag="VFN58_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HET8916968.1"
                     /translation="GTDHAPHTKEEKDIGWKDMWKSPGGEPQIQDYLSLFLTAVNEGK
                     LTLDTLVRITSYNPARIFGVYPRKGVIQPGADADMVIVDMNKEEVISNETTYTKVGWT
                     PYHGRKVKGVPIRTIVRGKTVMADGKIVGKPGDGQFITPVG"
BASE COUNT          540 a          600 c          572 g          538 t
ORIGIN      
        1 ttggcccgtg gatcaccacc aaagatgaag tatccgatcc tcacaatctt tggatgaagc
       61 tttggattaa cggcgaagtc aaacaatcgg caaagaccga cgcgatgatc aatgatatcg
      121 cgacacaggt gagtttttta tcgcaagtca cgaccttgta tccgggcgat ctgatcacga
      181 ccggaaatcc ggactctccg gaattccaag aaaagctcaa gcccggcgat gttttgaaag
      241 ccgagatcga aggcatcggc gccatgagtc ttggcgtggc gaaggaaagc tgaggaattt
      301 tagtttacat gcaatcttgt tttcatggcc tcacaatgac ttttgcgaag ggaccgtgta
      361 tgctcacagc tagttagttt tatgagttaa tgactgccag cccgattgtc gattctctgg
      421 caagtttacc gttacaagga actcgttagt ctatggtacg tatcgcaccg tttcgcgggc
      481 tattctacaa ccacaaaaag attcgcgatc tttccaaggt gattgctccg ccctatgacg
      541 tgatctcaaa agaggagcaa gataaacttt accgcaagtc gccgtataat tttgttcgcc
      601 tggatctcaa ccaagagccc gactcctatt cggcggtcgc tcagcttttt caagaatggc
      661 aagctgaagg aatttttgaa cgggatgaag tcccggctat ctattacagc acgcaccgct
      721 tctctgtcag aggcggggag gagagagagc gtcaaggctt cttcgctctt accgaattac
      781 aggacttctc cggcggcgac attcgccccc acgaaaagac gctagacgcc cccaaagagg
      841 accggctcaa actgatgctt gcctgccacg cgcagctgag cccgattttc gccctttatg
      901 ccgagcccaa atcgtcggtc aaccgcatgc tggctgccgg catcgaaggg attgatcctt
      961 tcattgaggt caaacaagac ggtaccggcg agtgccgcct ctggcgggtt accgatccgg
     1021 agcttgttcg ccgggttcag caagagatga gagatcagcc tctgctgatc gcggatggcc
     1081 accaccgcta tgaagccacg ctcaagtacc gcgatcaggt acgccgggaa caaagtcaat
     1141 ggaatggccg tgaggcgttc aactatatca tggcttattt tgccaacatg gatgacgaga
     1201 acctggtgat tcttcccacc catcgtctgg tgcggggtta cgctccgcag ccgttcttgc
     1261 agttggagga taccctgcaa aaatattttt acgtcgagca atatccgaaa acgccggaag
     1321 gcaaaggctg gtttctgaag gcacttaaaa gtggcgccaa aaaacagcgc ctcatcggcg
     1381 ccagtttcaa gcgtgatccg cgctacctga ttttgcggct gaaaaacaaa aggatcatgc
     1441 agcgcctggc caaagatcta agcgagccgc tccgcgaact cgatgtgacg actcttcatc
     1501 tattgatctt agagcatatc ctcggcctcg ctccggacca gcaagtcagt ggcgagacga
     1561 ttcgttattc ccaggatgaa gaagcgatgc ttcaggccct ggagaaagaa gatttccaag
     1621 ctgcattcat tcttaatccg ccgaaggcgg aagaaatatt gacgatcgcc aacggcggcg
     1681 agaaaatgcc gcagaaatcg acttacttct atccgaagct catttccggt ttgatcgtta
     1741 acaagatcga cccgcaagag gaaattgaag accctccggc caagtaggat tttttaagac
     1801 ctcagcggcg aatctctcta ccccaccggt gttatgaact ggccatcacc tggctttccg
     1861 actatcttgc catccgccat cacggttttc ccgcgtacga tcgtgcggat cgggacgcct
     1921 ttaactttcc ggccgtggta cggcgtccat ccgactttgg tataagtcgt ctcgttgctg
     1981 attacttctt ctttattcat gtcgacgatt accatgtctg cgtcggcgcc cggttggatg
     2041 acacctttcc tcgggtacac gccgaagatg cgagcgggat tgtacgacgt aatgcgaacg
     2101 agggtatcaa gcgttagctt tccttcatta acggcagtga gaaacaggct gagatagtcc
     2161 tgaatctgcg gctcgcctcc cggcgatttc cacatatcct tccagccaat gtctttctct
     2221 tcctttgtgt gcggcgcgtg atccgtgccg
//
LOCUS       DASSWZ010000113         6602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_470589, whole genome shotgun sequence.
ACCESSION   DASSWZ010000113 DASSWZ010000000
VERSION     DASSWZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470589"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3527
                     /locus_tag="VFN58_02890"
     CDS             <1..3527
                     /locus_tag="VFN58_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916969.1"
                     /translation="ASEATRIRETLQAELVALRTTVEQKNLSLQQDHAATRELAERLT
                     AQLHDLQGELAEKQRLLEIRNQELGELTARTSDLQEQITCLELANKQTVKEAKAAARA
                     IEDSLRVRVQELEASVSEKAQLLQNRTAELESAQAETALLRERTNQLELTEAQTEAAQ
                     NEAERIHEALRNQLGSAQRALEDKDASLAQREAKFKESSEHFHAQLRDLQSQLTEKQQ
                     LLDSKDRDLSHAAGQITGLQDRIAQVESLHAEAQAAAATEVERLRRESQSEVAALQTT
                     LREKEQALQQRHAAIAEFEANLNDQLQDLRNQLTQKQERLDRRDQEFQTIGSEAAVLR
                     ERIAQLESAASEAGRLAAAEAERIRGELQAELGALQAHLKEKDLELAESQALVRESGG
                     RLQAQIHDQQIQLTERQLLLETRNVEIADLQNKLTAASEQLARFEAGQHEAAAAASNA
                     DLARQRFEAELAARHEELQNLQRLLADREAQSYDLRQSFNTQLHDLQGELAEKQGLIE
                     ARSQEVGELTARTSDLQEQITYLELTNKQTVKEANAAARALEDSLRARVRELEALVSE
                     KAQGLQNRTVELESAQSETALLRERIQRLELTGAQTEEAKNEAQRIGETLQNELSNVH
                     KALEQKDRSFAQQETEFRESTERLHVQLRDLQSQLTEERAALESQKGELQGARSEIAV
                     LRNQIHEIESAKTAAETNASVGIEHFREQYQIELAGLRADLDQSQLVLDERQTTIRAL
                     EKKLKSETHRLEAQLAEKQTLLNRGHLELQEKRSEISSLREEIAQSEFARRQTEMLAA
                     TQAEQIRERVKAEVGALDSQLKETESTLKVLADRARESESVFNARIADLQRHVAEKQK
                     LIEGQDVELTDFRSRISSLLAQVNDLERINTEALEQQRVAASRLEQDLRLHVTELQTQ
                     LAEKLIGLETRDDEIQALESKITGLVERIGQAEVARQQAEVTATSEIEQIRRQSQEEI
                     AARQLENDQKAEALQQHEARWDSTEQNLRLEINGLRAEVAEKHSLLENRNDELLRVKT
                     ELDVLQERIADLESAVKQPGRESLNKPEHTGEHSTTAVDSLWEALGQKEPVLEERQAA
                     VNDLEQNFQAQIDSLRSELAEKEALLENPNKGFLMGEPTLTESQKERLNRLEQLVEVI
                     KADNEQTLISPQNRRWRFSLGRKRRWKS"
     gene            3656..4378
                     /locus_tag="VFN58_02895"
     CDS             3656..4378
                     /locus_tag="VFN58_02895"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET8916970.1"
                     /translation="MIQTERVPTGIPGLDPLLEGGFPQGRSILVTGEPGTGKTIFSLQ
                     FLIEGLARGEKGIYVAADEGPMDILEQAASLGWDLEKHIEMKEIAILNAGTYLSSLPG
                     TGKDRHIDVHKAIGDLAGFVNRLEAKRLVLDPAGPFVLIRDTATRIQDQTRLLIKLLR
                     TSMPTTNILTSYAVPRTGERSMHGVEEYLVAGAIVLEMIWKEGQLARSLVIEKMRCTN
                     VKPTQYDIDIVKEQGIVLQPLP"
     gene            4461..5273
                     /locus_tag="VFN58_02900"
     CDS             4461..5273
                     /locus_tag="VFN58_02900"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HET8916971.1"
                     /translation="MAKQTDSEVVKRVSTGIAGLDSLLQGGFLPGRSYLVTGDAGTGK
                     TTACMQFLKSGLEQEEKAVYVTVDERPAEILQAADSLGWDLQQYVQAKSFAILDASPY
                     FSGRAGTVGEKGLDLPKIVSDLATYAKKLEATRLVIDPVTPLILAGDSPTRVQEHARM
                     LIHLLQSNLATTNFITSHLPPRANNDPTNGIEEFLAAGVLILKVTQDNDRPVRTLSVK
                     KMRGTAVEPNEYPFSIVKDHGIFLLGSDKKPIPTVVEEPGFQGLEFFQLPKE"
     gene            5382..>6602
                     /locus_tag="VFN58_02905"
     CDS             5382..>6602
                     /locus_tag="VFN58_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET8916972.1"
                     /translation="MRLDKLTTKSQEALQQAQSLAESRNHQAIDVEHLLFALLGQKEG
                     VVLSLLQKLGASTSSLTERLQKALDRVPQVTGSTGQTFITPRLKKIIEAAEAEAETLK
                     DDYVSAEHLLLAMLQDSGEAGKALKELGVSRDKILNALVSIRGAQRITDPNPEEKYQA
                     LEKYSRDLTDLARKGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LALRIVNGDVPEGLKEKRLVALDLGALVAGAKFRGEFEDRLKAVLKEVTEASGQIILF
                     IDELHTLVGAGAAEGAMDASNMLKPALARGELRCVGATTLDEYRKRVEKDPALERRFQ
                     PIYVGEPSVEDTIAILRGLKERYEVHHGVRITDGAIIAAATLSHRYISDRFLPDKAID
                     LIDEAASRLRIEIDS"
BASE COUNT         1798 a         1739 c         1838 g         1227 t
ORIGIN      
        1 cagccagcga agcaacacga attcgggaaa ccctgcaagc cgaactagtt gcgctgcgga
       61 cgacggttga gcaaaagaac ttatcactac aacaagatca cgcggctacg agggaattgg
      121 cagaacgcct caccgcgcag ctgcacgatt tgcaggggga gttggcagag aagcagaggc
      181 tgcttgagat tcgcaaccaa gagcttggcg aactcaccgc aaggacgagc gatctgcagg
      241 agcaaattac ctgtcttgag ctggccaaca aacagaccgt caaagaagct aaggccgcgg
      301 cccgggcaat agaagacagc ctgcgtgtcc gcgtccaaga attagaagcg agcgtttcag
      361 aaaaagcgca actcttgcag aaccgcacgg ctgagcttga aagcgcacag gcggaaacag
      421 ccctgttgcg cgagcgcacc aatcagcttg aactcaccga ggcccagaca gaggcggcgc
      481 agaacgaagc cgagcggatt cacgaggctc tgcggaacca gttaggcagt gctcaaagag
      541 ctttggaaga caaggatgct tcgttagcgc aacgtgaggc gaagttcaaa gagtctagcg
      601 agcacttcca tgcgcaactg cgcgacctgc aaagccaact gaccgaaaaa cagcaactgc
      661 tggactcaaa agatagggat ctgagccatg ctgccgggca gataactgga ctgcaggacc
      721 gcattgctca ggtggagtct ctccatgccg aggcgcaagc cgctgcggca accgaagttg
      781 agcgcctccg ccgggaatcc caatccgagg tagcagcgct gcaaacgacg cttcgagaaa
      841 aggagcaggc gctccagcag cgccatgcag cgattgctga attcgaagcg aatcttaacg
      901 accaacttca agacctcaga aatcaattaa cacaaaaaca agaacgattg gaccgtcgcg
      961 atcaggagtt tcaaactatt ggttccgaag cggcggtgct tcgcgagcgc attgcacagt
     1021 tggagtcagc cgcttctgag gcggggagat tggcagcagc agaagcagag cgcattcgcg
     1081 gtgagctcca ggcagaattg ggtgcgctcc aagctcacct caaagagaaa gaccttgagt
     1141 tagccgaaag ccaagcgttg gttcgagagt ctgggggtcg actgcaggca cagatccacg
     1201 accaacaaat ccagctaacc gaaagacagt tgttgctcga aacccgcaat gttgagattg
     1261 ccgatcttca aaacaaattg actgccgcct cggagcagtt ggcaagattt gaagctggcc
     1321 aacacgaggc tgcagcggcc gccagtaacg cggatctcgc tcgccaaaga tttgaagcgg
     1381 agttggctgc gcgccacgaa gaattgcaga atctgcagcg actactggcg gatcgtgagg
     1441 cacaatctta cgacttgcgg caaagcttca acactcagct gcacgatttg cagggagagt
     1501 tggcggaaaa gcaggggctg attgaagctc gcagccaaga ggttggcgaa ctcaccgcaa
     1561 ggacgagcga tctgcaggag caaattacct atctcgaact gaccaacaaa cagaccgtta
     1621 aagaagctaa cgccgccgct cgggcgctcg aagacagtct gcgtgcccgc gtccgagaat
     1681 tagaagcgct tgtttcagaa aaagcccaag ggttgcagaa ccgcacggtg gagcttgaaa
     1741 gcgcgcagtc cgagactgct ctgttacgag agcgcatcca acggcttgaa ctgaccggcg
     1801 cacagacgga ggaggccaag aacgaagccc agcgcatagg cgagacgctg cagaacgaat
     1861 taagtaacgt tcacaaagcg ctggagcaaa aggataggtc cttcgcgcaa caggagacgg
     1921 aattcaggga atccaccgag cgtctccatg tgcaactgcg cgacctccaa agccaactga
     1981 cggaagagcg tgcggccctc gaaagccaga agggggaact gcagggtgcc aggtccgaaa
     2041 tcgctgttct ccggaaccag atacacgaaa tagagtcagc caaaacggca gcggagacaa
     2101 atgcttctgt cgggatagag catttcagag aacaatatca gattgaacta gccggtctga
     2161 gagccgatct cgaccagagc cagttggtcc tcgatgagcg tcagacaacg attcgagcgt
     2221 tggagaagaa gctcaaaagc gagacccacc gactcgaggc tcagctagcg gagaagcaaa
     2281 ctttactgaa tcgcggtcac ctagaactcc aggaaaagag gtcggaaata tctagccttc
     2341 gggaagaaat agctcaatca gagttcgctc gcaggcaaac cgagatgctc gccgcaacgc
     2401 aggcagaaca aattcgcgag cgcgttaaag cagaagttgg ggcgctagac tcccagctga
     2461 aggaaacaga aagcactctt aaagtcctgg ccgatcgtgc acgtgagtcg gaatcggtct
     2521 ttaatgccag gatcgccgat ctgcagcggc acgtcgccga aaaacagaag ctcatcgaag
     2581 gccaagatgt tgagctgact gattttcgaa gccggatcag cagtctcctg gcacaagtca
     2641 atgatctgga acgcatcaac acagaagcgc ttgaacaaca acgggtcgcc gccagccgct
     2701 tggagcaaga ccttcgcctc catgtcactg aattgcaaac ccaattagcc gaaaaactga
     2761 tcgggcttga aacccgcgac gatgagattc aagcccttga gtccaagatc accgggctag
     2821 tcgagcgtat tggtcaagcg gaagtcgctc gtcaacaggc agaagtaaca gcgactagcg
     2881 aaatagagca aatacgtcgg cagtctcagg aagagatcgc agctcggcaa ttggaaaacg
     2941 accagaaggc tgaagccctg caacaacacg aggcgcgatg ggattctacg gaacagaatc
     3001 ttcgacttga aatcaacggt ttgcgcgcgg aagtcgcaga gaagcattct ttgctcgaaa
     3061 accgcaatga cgaactgctg cgggtcaaaa cggagctgga tgtcctccaa gaacggatcg
     3121 ccgatctaga gtccgcagtg aagcagcctg ggcgtgaatc tctgaacaag cctgagcaca
     3181 cgggggagca ttccacgacc gcggtggaca gcctctggga ggcgttgggt cagaaggaac
     3241 cggtattgga agagcgacaa gcggcagtca acgatttgga gcaaaacttt caagcacaaa
     3301 ttgacagtct acgtagtgaa ttggcagaaa aagaagccct gcttgagaat ccaaacaaag
     3361 gctttcttat gggagaacca actcttacgg agtcccaaaa agaaagattg aaccggcttg
     3421 aacagctggt ggaagtcatt aaggccgaca acgaacaaac actgatatcg ccccaaaaca
     3481 ggaggtggcg cttcagcctg ggccggaaac gtcgatggaa gtcttgaagc cgatacgtca
     3541 agcaaaagct tcgcctggtt ttgtcggtta cgagtcgaaa gacgagagcg atggtgagtt
     3601 aagagcaatt cattttgaat tatatagttt agggagaatc agctaagaga caattatgat
     3661 ccagacagaa cgtgtaccga ccggtatccc ggggcttgat ccgctgctcg agggtggttt
     3721 tcctcaagga cgatctattc tcgtcacggg tgaaccggga acgggtaaga ccattttttc
     3781 actgcagttc ctcattgaag ggctggcccg cggagaaaaa gggatctatg tcgccgcgga
     3841 tgaaggaccc atggatattc tcgaacaagc ggcctccctg ggatgggatc tggagaagca
     3901 tatcgagatg aaagaaatag cgattttgaa tgccggcacc tacttgagtt cattacccgg
     3961 gaccggaaaa gataggcata tagatgttca caaggcaatc ggcgaccttg ccggtttcgt
     4021 caatagactg gaagcaaagc ggctcgtatt ggaccctgct ggacctttcg ttctaatacg
     4081 tgacacggcg actcggatac aagatcagac gcgtctgctt attaaactgc tacggacctc
     4141 gatgcctaca accaatatcc tgacctctta cgccgtgccg cgcaccggcg agcggtccat
     4201 gcatggtgtc gaagaatacc ttgtggccgg cgcgattgtt ctcgagatga tctggaaaga
     4261 aggacagttg gcccgcagtc tcgtcattga aaaaatgcgt tgcacaaacg taaagcctac
     4321 gcagtatgat atcgacatag tcaaagagca aggcatcgtt ctccaacccc tgccgtgagc
     4381 gcgtaaactc accctggctc aaaattgggc gcgatttgat cgaaaattag ctgcagcgaa
     4441 ttgtctaatc ggagagaacg ttggctaaac aaaccgattc cgaagtcgtc aaacgagtgt
     4501 caaccgggat cgcaggttta gattctctac tacagggagg tttcctgcca ggacgttcct
     4561 atctcgtgac cggcgacgcc ggtaccggaa agaccaccgc ttgcatgcag ttcttgaaaa
     4621 gcggcctcga gcaggaggaa aaggccgttt acgtaacggt tgacgaacgg ccagcagaaa
     4681 ttttgcaagc ggccgattcg ctgggctggg acctccaaca atacgtgcaa gcaaagagct
     4741 tcgcgatttt ggacgcctcg ccctatttca gcggtcgagc cggaaccgta ggggaaaagg
     4801 gtctggatct gccgaagatt gtgtcggatc tcgcgactta tgcaaagaag ttggaagcga
     4861 ctcgacttgt tattgacccc gtcaccccgc taatcttggc cggtgattcg ccgacccgcg
     4921 tacaagaaca tgccagaatg ttgattcatt tgcttcaatc caatctggcc acgacaaact
     4981 tcattacttc acatcttccg ccgcgggcaa acaacgatcc caccaatggc attgaagagt
     5041 ttcttgccgc gggggttttg attctgaaag tgacgcaaga caacgaccgg ccagtgcgaa
     5101 ccctgagcgt gaagaaaatg cgcggcactg cagtggagcc caacgagtat cccttcagta
     5161 tcgttaaaga ccatgggatc tttctcctcg gctcggacaa aaaacccatt cctaccgtgg
     5221 tcgaggaacc cggtttccag ggactcgaat ttttccagct tcccaaggaa tagcatttcg
     5281 aaccttctcg ctcggtatca ctcctccata aaacttctac cgggcttcct tgacagcctt
     5341 tgaaacgatg cttaactttt aggcattcgt gaggagaagc catgcgtctt gacaaattaa
     5401 ctacaaaatc acaggaagcg ctgcagcaag ctcaatcgct agctgagagc cgcaaccacc
     5461 aagccatcga tgtagaacac cttctttttg ccttgttagg ccagaaggaa ggggtggtct
     5521 tatcactcct acaaaagctc ggcgcgtcca ccagctcgct tacggagcgg ctccagaagg
     5581 ccctcgaccg cgtcccgcaa gttaccgggt cgaccggcca aactttcatc acgccgcgcc
     5641 ttaagaaaat cattgaagct gcggaagccg aagccgaaac actcaaagac gattacgttt
     5701 cagccgagca tttgcttctc gcaatgctcc aggacagcgg cgaagccggt aaggccctta
     5761 aagagctcgg agtgtcgcgc gataaaattt tgaacgcgct ggtcagcatt cgcggcgccc
     5821 aacgcatcac cgatcctaac ccagaggaga aatatcaggc tctggagaaa tacagccgcg
     5881 acctgacgga tctggctcgc aagggaaaac ttgatccggt catcgggcgc gatgatgaga
     5941 ttcgtcgggt gatccaggtc ctgtcacgtc gaaccaaaaa caatcctgtg ctgatcggcg
     6001 aacctggtgt aggaaaaacc gccattgtcg aagggctcgc gctccgaatc gtcaacggcg
     6061 acgtccccga aggattgaag gaaaagcggc ttgtggcgct cgatctgggc gcgctggtgg
     6121 ccggcgccaa attccgcggc gaatttgaag accggctgaa ggcggttttg aaggaagtta
     6181 ccgaggccag cggacagatc attctcttca tcgatgaact ccataccctg gtgggcgccg
     6241 gtgcggccga aggggcgatg gacgcttcga atatgttgaa accggccctc gctcgcggcg
     6301 agttgcgctg cgtcggagcc accaccctcg atgaatatcg caagcgcgtc gagaaagacc
     6361 cagccctaga acgccggttc cagccgatct atgtcggcga gccttccgtt gaagatacca
     6421 tcgcgatctt gcgcggtctc aaggaaaggt acgaagtcca tcacggcgtt cgcatcaccg
     6481 acggtgccat tatcgccgcg gccaccctgt cccatcgcta catcagtgat cgtttcttgc
     6541 cggataaagc catcgatcta attgacgagg cggcatcgcg gctacgaatc gaaatcgaca
     6601 gc
//
LOCUS       DASSWZ010000114         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_470603, whole genome shotgun sequence.
ACCESSION   DASSWZ010000114 DASSWZ010000000
VERSION     DASSWZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470603"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFN58_02910"
     CDS             complement(<1..482)
                     /locus_tag="VFN58_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HET8916973.1"
                     /translation="MQLTTVKIEKPDDINFILGQSHFVKTVEDIHESMVQTVPGIKFG
                     LAFCESSGEMLVRWTGTDDSMIELAKRNALALSAGHSFILFLGPGFFPVNVLNPIKMV
                     PEVCRIFCATANPVEVVIAETDQGRGILGVIDGGKSKGVEDAQGIAWRKDLLRKIGYK
                     L"
     gene            complement(526..1428)
                     /locus_tag="VFN58_02915"
     CDS             complement(526..1428)
                     /locus_tag="VFN58_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8916974.1"
                     /translation="MFTQMLIPLDGSKTAEKVLPYARFVAGALKLPVELLAVVDIVEM
                     ATHITAARASHLDTVIEDSVRNSEQYLRGVAGTFPGGTKCTVEKGKAEQVIIETAAAD
                     KGMIITMATHGRSGINRWLLGSIAEKVLRGTTNPLLLVRATEEAKAETVASLKSIVVP
                     LDGSELAESVLPAVAELAKTLKLAVVLFRAYNIPSSAYAGDEGYYAINYDEMLTAMRD
                     EAVDYLEKKTEAVKKMGIANVSSVAKEGFAADEIISLSRQSPDNLIAMSTHGRSGVKR
                     WVLGSVTETIVRHSADPVLVIRAN"
     gene            1614..>3193
                     /locus_tag="VFN58_02920"
     CDS             1614..>3193
                     /locus_tag="VFN58_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8916975.1"
                     /translation="MIVRLTSMFFFSVPDLGFDNNLVSRKAAFDRVGWFMIGGKKVDS
                     LRSYMIRMQTGREENATMESDETTKDLSSKDLITTRATAEGATDSTGPGTKRAREENL
                     SPSWDLVADVVVVGAGATGLPAAIKAVDGGASVIVVETNYDIGGHAIISGGNTPLGGG
                     TSAQRKYGIEDSPDLVFRDLTDWSIVQPNGWPDYRYNDRAVMRAFADHCVPTYDFLVA
                     NGADFKDVPPDNQGGHNLGNSAPRENHAIWTKGAGPESPNARAGTGLIRPLEASARAK
                     GVRFLLNYKMASLIREPSSGQKAGRVIGITAQYTPRIMPGQTTPLKGFRSNGNIETTQ
                     PSVAIRAKKAVILATGGSTGNTNFRRMFDPRLTEVLQLAGEPYTFQDGSGELAAMAIG
                     ASLWGFANQILENGDNIRTKRALATKYNYMTWALETPIFPLVRATGLDVKDWQDLILV
                     NQVGKRFYDETKGDYPHGNVYDEVKPYTPNDYRNNENIKFSPTKYNFFNAAVAMNEYS
                     EPPDYSAGPVWAIFDADAV"
BASE COUNT          742 a          920 c          853 g          678 t
ORIGIN      
        1 agtttgtaac cgattttccg cagcaaatcc ttgcgccagg caatgccttg ggcgtcttcg
       61 acgcctttgc tcttgccgcc gtcgataacc cccagaatgc cgcgtccctg gtcggtctcg
      121 gcaattacga cttcaactgg attcgcagtg gcgcagaaga tccggcatac ctcgggaacc
      181 attttgatag ggttaaggac gttgacaggg aagaagcctg gtccgaggaa gagaatgaaa
      241 ctgtgaccgg ctgagagggc gagagcattg cgcttcgcca gctcaatcat ggagtcatcc
      301 gtgccggtcc aacgcaccag catttcgccg gaggactcgc agaaggcgag accgaatttg
      361 attccgggaa ccgtctgaac catcgattcg tgaatgtcct cgacggtctt gacgaagtgg
      421 ctctgcccca gaataaaatt gatatcgtcc ggcttttcga tctttaccgt tgtaagttgc
      481 atatcatgtc gccatggcaa tgatacaggc cgcgtcgctc ccgcatcaat tcgcacgaat
      541 aactagaacc ggatcggcag agtggcgcac gatagtttcg gttacgctgc ccaacaccca
      601 tcgtttcacg cccgaccgtc cgtgagtgga catagcgatc agattgtctg gagattgacg
      661 cgacagggaa atgatctcat ctgcagcaaa gccttctttg gcgacagagg aaacgttcgc
      721 aatccccatc tttttcaccg cttcggtctt tttctccaga tagtcaaccg cttcgtcgcg
      781 cattgcggtg agcatttcgt catagtttat cgcatagtag ccctcgtcac cggcgtaggc
      841 gctggaggga atgttgtaag cccggaataa cacaaccgcc agcttcaatg tctttgccag
      901 ttccgctacc gcgggcaaca cactctctgc tagctcggag ccatccaacg gcaccacgat
      961 agatttcaaa ctcgccacgg tctccgcctt cgcctcctcc gtggctctga ctaggagtaa
     1021 tgggttcgtc gtaccgcgca aaaccttttc ggcaatactc cccaaaagcc accggttaat
     1081 tcccgaacgg ccatgggtcg ccatagtgat aatcattcct ttgtccgccg ccgcggtttc
     1141 gatgatcacc tgttcggcct tgcccttctc caccgtgcat ttcgtaccgc cgggaaaagt
     1201 accggcaacc cctctcaggt attgttcact gttccgcacg ctatcttcaa tcacggtatc
     1261 cagatgggaa gcccgggcgg cggtgatatg ggttgccatt tcgacaatgt cgacaactgc
     1321 cagaagctct acgggaagct ttaatgcccc cgcgacaaaa cgagcgtagg gcaatacttt
     1381 ttcagccgtc tttgacccgt ccagcggtat cagcatttgg gtaaacatgg ggacctcctc
     1441 gtaactaccg cgcgctgcat cactccactt tgacttccac tttctggcta tgcctcgttt
     1501 atgttagata gcccaaactt acaagattcc actattaaaa gttgtcctaa ccttatccgc
     1561 atgaacatcc tttggcaact caagaatcct cacaatccct tgatggtgag cacgtgatcg
     1621 taaggttgac ttccatgttt tttttctcag ttccggacct cggctttgac aacaatctcg
     1681 tcagccgcaa agctgcgttc gaccgcgttg ggtggttcat gattggcgga aagaaagttg
     1741 attcgcttcg cagttatatg atacggatgc aaactggccg ggaggaaaac gcaaccatgg
     1801 aatcagacga aacaacgaaa gatctctcaa gcaaggatct aatcaccacg agagcgacag
     1861 cggaaggcgc gaccgactct acgggtccgg gaacaaagag ggctagagaa gaaaatctct
     1921 ctccgagctg ggacctggtg gcggatgtgg tcgtggtcgg cgcgggggcg acgggcttgc
     1981 cggcggccat taaggcagtt gacggaggag cctcagtcat cgttgtcgaa acgaattacg
     2041 atatcggcgg acacgcgatc atcagcggcg gcaatacacc cctgggtggc ggtacaagcg
     2101 cgcagaggaa gtacggtatc gaagactcgc ccgacctggt tttccgcgat ctcacagact
     2161 ggtccatcgt gcagcccaac ggctggcccg attaccggta taacgaccgc gcggtgatgc
     2221 gcgctttcgc cgatcattgc gtcccgactt acgactttct ggtggccaac ggcgcggact
     2281 tcaaggatgt ccccccggac aatcagggcg ggcacaattt gggaaactct gcgccgcgag
     2341 agaatcatgc gatctggacc aaaggcgcgg ggccggaaag cccaaacgca agagcgggca
     2401 ccggtctcat caggccgctc gaagcgagcg cgcgcgcaaa gggcgtgcga tttttgctca
     2461 actataaaat ggcaagcctg atccgcgagc cctcctcggg acaaaaagcg gggagagtga
     2521 tcggcatcac ggcacagtac acgccgagaa tcatgccggg ccagacgaca ccgttaaagg
     2581 gctttcgctc caatggcaat attgagacta cccaaccgag cgtcgcgatc cgcgcgaaga
     2641 aggctgtgat cctcgccacc ggcgggagca ccggcaacac aaacttccgc cgcatgttcg
     2701 atccgcggtt gactgaagtg ctgcaacttg cgggtgagcc ctacacgttt caagacggca
     2761 gcggcgagct tgccgccatg gctatcggtg cctcgctctg gggctttgcg aaccagatcc
     2821 ttgaaaacgg cgataacatt cgcactaagc gcgcgctcgc cacgaagtat aattacatga
     2881 cgtgggcgct tgaaactccg attttcccgc tcgtccgcgc caccggcttg gacgtgaagg
     2941 attggcagga cttaatcctg gtcaatcaag tcggcaaaag attctacgac gagaccaaag
     3001 gggattaccc acacggcaac gtgtacgacg aagtcaagcc ctacacgccc aacgactatc
     3061 gcaataacga aaatatcaag ttcagtccca ccaaatacaa ctttttcaac gccgcggtgg
     3121 cgatgaacga gtactcggaa ccacccgact attccgccgg gccggtgtgg gccatattcg
     3181 acgccgacgc ggt
//
LOCUS       DASSWZ010000115         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_923375, whole genome shotgun sequence.
ACCESSION   DASSWZ010000115 DASSWZ010000000
VERSION     DASSWZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923375"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFN58_02925"
     CDS             <1..819
                     /locus_tag="VFN58_02925"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8916976.1"
                     /translation="GPVLTVQEKRRVYESVGKIARKAGRVHIATIAPQSTDEAINLVR
                     DLETLPVDAAMIFPPLLFAWGKVEGELKFRFFEDLTHSTSLPLVLFQIPVKSYWYDVE
                     TICRIAKLEKVVAFKEASFDMKLFTDTMQQLERQNAIMEVLTGNDRFVAESYRLGAAG
                     ALIGVSNVATEKWAAMDTAGRAGDFDNAAAIQKELESLKELIFTEPIVEAVARIKIIL
                     AHEGLIDTATVRRPQLGVSESEKKQLLDSYRALKEIGYRSAANLSAARSSGTAR"
     gene            complement(853..2142)
                     /locus_tag="VFN58_02930"
     CDS             complement(853..2142)
                     /locus_tag="VFN58_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8916977.1"
                     /translation="MTTNPPPSATRSPALSKRQIAFAALRHRDYRRYFFVIMLSQMGD
                     NIEHVISYWLLFQKFHSPALGGFAVISHWTPALFFAVYFGALADRHDCRRVLQAAQLM
                     HAGVSLAWGILFLTDTIQVWHACVLLIIHGMAGVVGGPGSQLIIHDIVGREYLQSAVR
                     LNSTGRNIAVLFGPAVGGATMLLFGPPMGLFINTLMYLPMFILMFILPYTGHGRDEAG
                     SARAVRFSLSGALRLMRETAANPTIFSMILLGGATSLLVGNAFQPQMPEFAHDLGHDH
                     QDWAYSMLLAANAAGSVVGGLLLEGTGLLRPNARNAAICSILWCAAIIGFAVSTSYPL
                     AVALLFCAGFLNLTFLSMAQTLVQLGAPPQLRGRLIGLFNMSHNGLRAFSGVTVGVIG
                     GLIGIHWSLAISAMTLLAVTIALFALVIPGRGSREYS"
     gene            complement(2236..>2510)
                     /locus_tag="VFN58_02935"
     CDS             complement(2236..>2510)
                     /locus_tag="VFN58_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916978.1"
                     /translation="FAAGVLNLTFSSMGQTIVQLLSPPQLRGRLIGLFAMASLGLKAF
                     SGLTVGVLGGLIGVHWSLALSAMVLLAITIGLFAFTIPGQQHGEFS"
BASE COUNT          644 a          715 c          681 g          470 t
ORIGIN      
        1 ggaccggtac tcacggtgca ggagaaacgc cgagtttatg aaagcgtcgg caaaatagcg
       61 cgcaaggccg gccgggttca catagcaacc atcgcaccgc aatccacaga cgaggccatc
      121 aacctggtgc gtgacctgga aacgctgcca gtggatgcag ccatgatctt tccgcctttg
      181 cttttcgcct ggggtaaagt ggaaggagag cttaaattcc gttttttcga agatctcact
      241 cacagcacaa gtctgccgct ggtgctcttt cagatcccgg tcaagagcta ctggtatgat
      301 gtcgaaacca tctgccgcat cgccaagttg gaaaaagtcg tcgccttcaa ggaagcctcc
      361 tttgacatga aacttttcac ggacaccatg cagcaactgg aacgccaaaa cgcaatcatg
      421 gaagtcctca caggcaacga tcgcttcgtt gccgagagct atcgcctcgg cgctgctggt
      481 gcgctgatcg gggtgtcgaa cgttgcgacg gaaaagtggg cggctatgga taccgccggc
      541 cgcgcgggcg attttgacaa cgctgcggcg attcaaaaag aactggaatc cttaaaggag
      601 ctaatattca ctgaacccat cgtcgaagcc gtggcgcgaa tcaaaatcat tctagcccat
      661 gaagggttga tcgacaccgc caccgtccgc cgcccgcagc tcggtgtgag cgaatcggag
      721 aaaaaacagc tgctcgacag ctatcgggct ttaaaggaaa taggctaccg ttcagcagcc
      781 aatctgtccg cggcgaggag cagtggtacg gctcgataaa aaccgccacg agtttagttg
      841 gaagggagtt gttcaactgt attcccggct tcctcgtccg ggaataacca aggcgaacaa
      901 cgcgatggtt accgccaaca atgtcatggc gctgatcgcc agcgaccagt gaattccgat
      961 cagtccgccg atcactccca ccgtaacgcc gctaaaagct cgaaggccat tgtgcgacat
     1021 attaaacaga ccgatcaatc ggccgcgcag ttggggcggc gccccgagct gcacgagagt
     1081 ttgcgccatg gagagaaaag tcaaattgag aaaccccgcg caaaaaagca atgctaccgc
     1141 cagcggatag ctcgtggaaa cggcgaaacc tattatggcc gcacaccata gaatcgagca
     1201 gatcgccgcg ttgcgcgcgt tcggtctaag tagccccgtg ccttccaata ggagaccacc
     1261 caccacggaa ccggcagcat tggccgcgag gagcatgctg taggcccaat cttgatggtc
     1321 gtgtcctaaa tcgtgcgcaa actcgggcat ctgcggttgg aatgcgttac cgaccaacaa
     1381 tgacgtagcc cctcccaaga gaatcatcga aaaaatagtt ggattcgccg cggtctcccg
     1441 catgagccgc agcgcgccgc tcaagctaaa ccgcactgcc cgggcacttc cagcctcatc
     1501 ccggccatgg ccggtataag gcaaaatgaa cattaagatg aacatcggca gatacatcag
     1561 agtattgatg aaaagtccca tcggtggtcc gaaaagcagc atcgttgctc cacccaccgc
     1621 cggcccaaaa agcaccgcaa tgttgcgtcc ggtggaattg agccgcacag cgctctgtag
     1681 gtattcacgc ccaacgatgt cgtgaataat gagttggctt cctggtccac ccactacgcc
     1741 ggccatgcca tggatgatca gcagaacgca ggcgtgccac acttgaatcg tatcggtaag
     1801 aaaaagaatg ccccatgcta gagagacccc ggcgtgcatc agttgggccg cctgaagtac
     1861 ccggcggcag tcgtgacggt cggcaagcgc gccaaaataa accgcaaaaa ataacgcggg
     1921 cgtccaatga ctgatgaccg caaagccccc cagcgccggt gagtgaaatt tttggaacaa
     1981 caaccagtaa ctgataacgt gctcgatgtt gtcgcccatc tgggacagca tgatcacaaa
     2041 aaagtatctt cggtaatccc gatggcgcaa tgccgcgaag gcaatctgtc gcttggacag
     2101 ggccggtgat ctggtagctg acggaggtgg attcgtcgtc atcttatttc ttggaattcc
     2161 gacacagata caaatcttgc gcgccagcga gcgagactcg tattggacta tttcatggct
     2221 tcaacgcccg gcccgttaac tgaattcccc atgctgctgc ccgggaatcg tgaaggcgaa
     2281 caacccgatc gtgatagcca gcagcaccat cgcgctcaat gccagggacc agtggacacc
     2341 gattagtcca cccaatacgc ccacggtgag gccgctaaac gctttcagcc ccaagctggc
     2401 catggcgaac aagccgatca ggcggccgcg aagctgtggt ggcgatagca gttgaacgat
     2461 ggtctgcccc atggaagaaa aggtcaggtt caacactccg gctgcgaaaa
//
LOCUS       DASSWZ010000116         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_513004, whole genome shotgun sequence.
ACCESSION   DASSWZ010000116 DASSWZ010000000
VERSION     DASSWZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513004"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /gene="mrdA"
                     /locus_tag="VFN58_02940"
     CDS             complement(<1..1457)
                     /gene="mrdA"
                     /locus_tag="VFN58_02940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET8916979.1"
                     /translation="MALLADINRQDGAPELRRRTRLLYLVLILVFGALLTRLFFLQLV
                     DGERYTFLSENNRVRIKRIPGTRGMVFDRQGQLLVDSRPSFDLLFVAEDAEEPEGTLR
                     QLARYLRRDEKELLAILEENKKRPAFEEIVIGKDVDWGTVVAVETHQIDLPGVSLRAR
                     PRRSYADGPVGAHVLGYLGEIGPKQLKILKEQGYATGDEFGQYGLEKTWEYFLRGQSG
                     GQQVEVDALGRRVRVLHEVTDVPGYTVHLTLDRQLQETAFEALKGKEGTIVALDVNTG
                     AILAMVSTPAFDPNVFARGIKSDEWQALMKDRLRPLSNRAIQGQYPPGSTFKVIMAIA
                     GLEEGVLQPESRISDPGYYYFGNRQFRDWKKGGHGSVDLHRAIVESCDTYFYQAGQRI
                     GVDKIAKWARAFGLGEKSGATLDDEKPGIVPDSEWKRKRFRQPWYPGETLSVAIGQGY
                     LTVTPLQLANMMAAVANGGTLYRPRVVDKIESVDGT"
     gene            complement(1466..1969)
                     /gene="mreD"
                     /locus_tag="VFN58_02945"
     CDS             complement(1466..1969)
                     /gene="mreD"
                     /locus_tag="VFN58_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET8916980.1"
                     /translation="MKLSLSFFVVGILLMLLQTTFLHLLPLGPVVPDLILVLCVYWGL
                     YHPTVGAALGSFLLGYSVDVVSSPILGLNAFAMSLVFLAVYLSSRSIWLHHPVVSSIV
                     VLLASLVKGAALILVWTVFLSVDGFWTGAIRYILMEALVAAVLAPVVFALLRRGQAYL
                     EQLRVTS"
     gene            complement(2003..2836)
                     /gene="mreC"
                     /locus_tag="VFN58_02950"
     CDS             complement(2003..2836)
                     /gene="mreC"
                     /locus_tag="VFN58_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET8916981.1"
                     /translation="MLAFLRKNQILLSSCFFLLLSLYILTAAARGQLKNDPIGPILLW
                     VMRPLQIAAQTTTNWLDEIQNSYSMLAGYKSENEKLRERIQELEVERNQLLEAEATNR
                     RLQELLDFRSHLPSGAVTASIIANSGSSWFKSCLLDKGSADGVRKGMAVVTPLGVVGQ
                     VVAVTARTAKVLLLADPNNGVDVLVQRTRARGIVSGSLDNGTILKYVKRSEDIQEGDR
                     LVTSGFDGVFPKGIVVGTVIKARKQTLGLFQHIEVMPAVSLARTEEVLIVKAEINQEK
                     N"
     gene            complement(2887..>3009)
                     /locus_tag="VFN58_02955"
     CDS             complement(2887..>3009)
                     /locus_tag="VFN58_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="functions in MreBCD complex in some organisms;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET8916982.1"
                     /translation="LLREETGLPVMMADDPLTAVVMGAGKALDEIALLREVAVH"
BASE COUNT          734 a          892 c          752 g          631 t
ORIGIN      
        1 gtgccatcca ctgattctat tttgtcaacg acccgagggc gataaagcgt cccgccgttg
       61 gctactgccg ccatcatatt ggcgagctgc agcggcgtca ccgtgagata gccttgacca
      121 atagccaccg agagggtctc ccccggatac cacggctgac ggaacctctt tcttttccat
      181 tcgctgtcag gaacgatgcc cggtttctcg tcatcgagag tagccccgct tttctctccc
      241 aatccgaagg cacgagccca cttcgcgatc ttatcaactc cgatccgctg gcctgcttgg
      301 tagaagtagg tgtcacaaga ctcgacaatt gcccggtgca aatccacaga accatgccca
      361 ccttttttcc agtcccggaa ttgacgattg ccgaagtaat agtagccggg atccgagatt
      421 cggctctctg gttgcaacac accctcttct aacccagcaa tggccatgat gaccttaaac
      481 gtggaaccgg gaggatactg accttgaatt gcccggttgc tcaaaggacg gagccggtct
      541 ttcatcagcg cttgccactc atcggatttg attccccgtg caaaaacatt ggggtcaaag
      601 gccggcgtgc tcaccatcgc gaggatagcg ccggtattga cgtcaagcgc aacgatggtt
      661 ccctccttac ccttgagggc ttcgaaggct gtctcctgaa gctggcgatc caacgttaag
      721 tgaactgtat agccgggaac gtcggtaact tcatgaagaa ctcgcacccg ccgcccgagt
      781 gcatccactt ccacctgttg accgccgctc tgaccgcgca gaaagtactc ccaggttttt
      841 tccaagccat actgtccaaa ttcgtcgcct gtcgcgtacc cctgctcttt caggatcttc
      901 agctgtttgg ggccgatttc gcccaagtat ccgagaacgt gagcaccgac cggtccatcg
      961 gcatagctgc gccgcggccg cgcccgtagc gaaactcccg gtaaatcgat ctgatgggtt
     1021 tccaccgcaa caacggtccc ccaatccacg tccttgccta tcacgatttc ctcgaaggcg
     1081 ggtcgttttt tgttctcttc gagaatggcg agcaactcct tctcgtcccg ccggaggtag
     1141 cgcgccaatt gacgcagcgt accctccggt tcttccgcgt cttcggcaac gaacagtaaa
     1201 tcgaaggacg ggcggctatc gacgagtagt tgcccttggc ggtcgaaaac cattcctcgt
     1261 gtgccgggaa ttcttttgat gcgcacgcga ttattctcag acagaaatgt gtaccgttca
     1321 ccatcgacca actggagaaa aaagagccgc gtgaggagag ctccgaatac aagaatcagg
     1381 acaaggtaaa ggaggcgagt tcgtctacga agctcagggg cgccgtcttg cctgttgata
     1441 tcggcgagca atgccatttt gattcttagg acgtgacgcg gagctgttcc aggtaggctt
     1501 gcccgcgccg taacagagcg aacacaaccg gcgccagcac cgcggcgaca agcgcttcca
     1561 tcaaaatata gcgaattgca cccgtccaaa agccatcgac gcttagaaac acagtccaaa
     1621 ccaatatcaa ggccgcgcct ttgaccagcg atgccaagag aacgacgatg gagctcacca
     1681 cgggatggtg cagccaaatg gaccgtgaac tgagatacac ggctagaaag acgagcgaca
     1741 tggcaaaagc gttcagacct agaatgggac tcgacacaac atccaccgag taacccaaca
     1801 agaaagatcc caaagcggct ccaacggtgg gatggtacag accccaatag acgcaaagca
     1861 caagaatcaa atcaggaacc acggggccga ggggaagaag atgaagaaaa gtcgtctgta
     1921 acagcatcag caatataccc acgacaaaaa acgatagcga cagtttcatc gtatatctat
     1981 gactaaatca ccggcgcgga tcctagtttt tttcttggtt gatctcagcc ttgacgataa
     2041 gaacttcttc ggttcgcgcc agacttaccg ccggcatgac ctcaatgtgc tgaaaaaggc
     2101 caagggtttg tttgcgcgct ttaatcacgg ttcccacgac gatgcccttg gggaaaacac
     2161 cgtcgaaacc cgaggtgacc agccggtctc cttcttgaat atcttcgctc cgcttcacgt
     2221 acttcaggat ggtgccgtta tcaagcgaac ccgaaacgat accgcgggcc cgcgttcgct
     2281 gaaccaaaac atccaccccg ttgttgggat cagcgagcaa taaaaccttt gccgtccgtg
     2341 ccgtcaccgc tacaacctgc ccgaccacgc ccaagggcgt aaccaccgcc atccccttgc
     2401 gcacgccgtc ggcacttccc ttgtccaaga gacagctttt aaaccaacta ctgccgctgt
     2461 tggcgataat cgaagcggtc accgcgcccg acggcagatg ggaacggaag tctaaaagtt
     2521 cctgcagtcg ccgattcgta gcctcggctt ccagcagttg attcctttca acctctaact
     2581 cttgaatccg ttctctaagc ttctcgttct cggacttata gcccgccaac atcgagtagc
     2641 tgttctgaat ctcatcgagc caattcgtgg ttgtctgggc agcgatctgc aacggacgca
     2701 tgacccacag cagtatggga ccgatcggat cattttttag ttggccgcgt gcggcagcag
     2761 tcaggatata aagcgacagg aggagaaaga aacaagagct taaaaggatt tggttcttgc
     2821 gaagaaaagc gagcatggaa ggctctttcg cctccaaaag gcgtggacca tagaggtctc
     2881 agaaactcag tgaaccgcca cctccctgag caacgcaatc tcatccaagg cctttcccgc
     2941 gcccatgacc acagccgtga gaggatcgtc cgccatcatg accggcaaac cggtttcttc
     3001 ccgcaacag
//
LOCUS       DASSWZ010000117         5334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1064968, whole genome shotgun sequence.
ACCESSION   DASSWZ010000117 DASSWZ010000000
VERSION     DASSWZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064968"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            608..964
                     /gene="queF"
                     /locus_tag="VFN58_02960"
     CDS             608..964
                     /gene="queF"
                     /locus_tag="VFN58_02960"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET8916983.1"
                     /translation="MATKPSKQLETFPNPHPQRDFEIAMECPEFTCICPRTGQPDFAT
                     IRIRYVPADLCVELKSLKLYLWSYRDEGAFHEAVINQILDDLVQACRPKTMTVVGDFN
                     VRGGIHTTVTAEYRAP"
     gene            1035..3035
                     /locus_tag="VFN58_02965"
     CDS             1035..3035
                     /locus_tag="VFN58_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET8916984.1"
                     /translation="MSDPIFLRELVIVLTATIAIVFVFQKLRLPNIVGFLLAGVIIGP
                     HGFQLIQSVGQVENLAEIGVVLLLFTIGLEFSLGTILSVQRRILWAGFLQVVLTTLIV
                     LGVARFLGVSVEVGLFYGFLVSLSSTAIVLRIYYDRGEINSLQGRLASGLLLFQDLCV
                     VPMMLLLPVLAGSGQDSIFLIVWVLAKSLITLVVIVWTARKLLPRLLHQVALLRNREI
                     FVLFVVLVCFGTAWLTSESGLSLALGALVAGLVISESELSHQIVADILPLRDCFSGIF
                     FISVGMLLNLGILSQDFRIALMELLLMIGIKSLVLFAVFWWLYRSVRLGVVLGLGLAQ
                     IGEFSFVLAKAGINFQLLSAADGQIFLAASILSMMATPFLIHWSHAWAFGFEGLFKDI
                     GFNRSTSGGANETPSATGHVIVVGYGLNGQNLARVLKEVGIPYRVLEMDPDLVRSAKA
                     GGEPISFGDGTRPELLQQVGIEKARVLVVAISDPAATARLVSQARRLRTDLYIIVRTR
                     YVAEIDHLYRLGANQVIPEEFETSVEIFARVLQEYHIPRNVISLQVDLIRKEHYGTLR
                     GIQLQGKQLDALSQFLVGTTSDIFSIIEKSPAVGKSLGEINLPSRSGVSVIAVVREGK
                     SYPNVDNDFKLAVGDMLVLLGGHKALDDAAQILNPSGDNNAS"
     gene            3227..4600
                     /gene="dnaB"
                     /locus_tag="VFN58_02970"
     CDS             3227..4600
                     /gene="dnaB"
                     /locus_tag="VFN58_02970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET8916985.1"
                     /translation="MASIDDNLRKVPPQNLEAESSVLGGILLENDAVNIVLELLRPED
                     FYRESHRKIFRAMIELTDRSEPVDLITLSECLKNRGEIDAVGGSAYLASLADFVPTAA
                     NISYYARIVREKSILRSLISTATEIATRGYEEQGNVEEFLDAAEKVIFDISEKKVKAS
                     FIAVGDMIKDTLKTVERLYERKEMVTGVPTGYEDLDKLTAGLQPSDLVIVAGRPSMGK
                     TAFSLNIATNAAFAGVGVAVFSLEMAKEQLVLRMLCSEAHVNNSKVRSGYLAERDFPK
                     LANAAGRLHEAPIYIDDTPAISVLELRAKARRLVRDRTKKVGLIVIDYLQLMRGMGAA
                     SNREQEISEISRSLKALAKELTVPVIAISQLNRRVEDRGDRRPMMSDLRESGAIEQDA
                     DVIMFIYRDEVYNQNSEKKGIAEIIVAKQRNGPIDTVELTFLNEFMRFENYTPRDEFD
                     YEEADEA"
     gene            complement(4625..5110)
                     /locus_tag="VFN58_02975"
     CDS             complement(4625..5110)
                     /locus_tag="VFN58_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET8916986.1"
                     /translation="MNGSTFTTSPDNRYFEDYVAGAVHEFGSIAVEEREVIDFGKRFV
                     PLSYHTDPELAKKSIYGRLIASGWHTAALMMRLYTDHYLSKVANLGSPGGDELKWDKP
                     VFPGDDLSIRVTVLEARRSESKPDRGIVRSFVEVLNQNREVVMSVKMVNFVRSRESAA
                     T"
BASE COUNT         1193 a         1364 c         1450 g         1327 t
ORIGIN      
        1 ctgccttccg acccctcgct gtatacggcg gctattttcg ccggcggtta atcctggcga
       61 taacttcgat gagcaaaaac ccggcaacgc tcgacagagc aaggaggaaa taaaagtagt
      121 ctggcatcgt tctccccttt ccgttgaaag agaaagagat agagcaatgc cgggaccgtc
      181 acgaaaaggt agggagaacg gtgttgattg ggataggctt cctaaagaat cctagtatgg
      241 caaacgccgt gatgccttag ccctaaaggc gaggtatggg aacgggcgaa ttagccttgg
      301 gtgacgggtg tcactaggtg acgcagtttc tacaaagtct catataagat gatttgtagg
      361 aagttaccaa aaacagcgtc actatccgtc atctatcacg cacttcgtac ctacaggatg
      421 tctttggctg ataaagaaaa acgggattaa ccaggaagaa tgagttctaa tgagggacaa
      481 agcagaaaag aataaaaacg aaataaaaac agagagctag accataattc cctgtcctca
      541 ttgccttctc ttccaaccag gcaaacaatt ggaataatat tcttgctgcg ttaagataat
      601 gcgcgatatg gccactaagc cgagcaaaca actggagact tttcccaacc cgcatccgca
      661 acgggacttt gagattgcga tggagtgccc cgaatttacc tgcatctgcc cgcgcacggg
      721 acaacctgat tttgccacca tccggattcg ttatgtgccg gcggacctgt gcgtggagct
      781 caaatcgctc aagctttacc tttggtctta tcgtgacgaa ggggcgtttc atgaagcggt
      841 cattaaccaa attttggacg atctcgtgca agcctgccgc cccaaaacga tgaccgtcgt
      901 cggtgatttc aacgtccgcg gcggcattca cacgacggtg accgccgagt atagagctcc
      961 ctgaagccat cccgatggtt tgtagctaag acctatttac tgtttataga cagacctccg
     1021 ccctactgct tgcgatgtct gacccaattt ttctccgcga gctcgttatc gtattgacgg
     1081 cgaccatcgc catcgtcttt gtctttcaga aattgcgtct gccaaatatc gtgggttttc
     1141 ttcttgccgg cgtgatcatc gggccccacg ggtttcaatt gatccaaagc gtcggtcaag
     1201 tggaaaactt ggccgaaatc ggtgtggtgc tgctgctttt taccatcggc ttggaattct
     1261 ccctggggac cattctatcg gtgcagcgtc gaatcctgtg ggcggggttc ctgcaggtcg
     1321 ttctgacgac tttgattgta ttgggcgtgg cgcgcttcct tggcgtatcg gtggaagtgg
     1381 gccttttcta cggatttcta gtctccttga gtagcacagc cattgtgctg cggatctact
     1441 acgatcgggg cgagatcaat tcgttgcaag gtcggttggc cagcggcctt ctgctgtttc
     1501 aagatctctg cgtcgtgccg atgatgctgt tactcccagt cctggcaggc tcgggacagg
     1561 attcgatctt tttgatcgtc tgggttctgg caaagtcgct gatcaccttg gtcgtcatcg
     1621 tctggacggc gcggaagtta ctgccgcgac tactccatca ggtcgcgttg ctgcgaaatc
     1681 gggagatctt tgtgcttttt gtggttctgg tgtgtttcgg aactgcctgg ttaacctcag
     1741 aatccgggct ttcgctagcc cttggagcgt tggttgcagg tctggtcatc tccgaatccg
     1801 aactcagtca ccagatcgtc gccgatattc tgcccctccg cgactgtttc agcgggattt
     1861 tctttatttc cgtcgggatg ctgttaaacc tcgggatttt gtctcaagac tttcgcattg
     1921 ctttaatgga gctgctgctg atgatcggca tcaaaagctt ggtgctcttt gccgtctttt
     1981 ggtggttata tcgttcggtt cgactagggg tcgtgctggg cttaggtttg gctcaaatcg
     2041 gcgagttttc attcgttctc gccaaggcgg gtatcaattt tcaacttctc agcgccgcgg
     2101 acggacagat tttcttggcg gcttcgattt taagcatgat ggcgaccccc tttcttatcc
     2161 attggtcgca cgcctgggca tttggtttcg aaggactctt taaggatatc ggattcaatc
     2221 gctcaacgag cggtggagca aatgaaaccc cctcggctac gggccacgtg attgtcgttg
     2281 gctacggatt gaacgggcaa aatctggcgc gggttctaaa agaagtcggt atcccctatc
     2341 gggtgctgga aatggacccc gatctggtac gttccgccaa agcgggcgga gagcccatct
     2401 ctttcggtga tgggacgcgg ccggaactat tgcagcaagt gggtatcgaa aaggcccgag
     2461 ttttggtcgt cgcgatctcc gatccagcgg ccacggcgcg tttggtgtcc caggctcgcc
     2521 ggttaaggac cgacctctat attatcgttc ggacccgcta tgtggcggag attgatcacc
     2581 tctaccggct tggggccaat caggtcatac ccgaagagtt cgagacgtcc gtagagattt
     2641 ttgcccgtgt tttgcaagaa tatcatatcc cgcgcaatgt gatctcgctt caggtggatc
     2701 tgattcgcaa ggaacactac ggtacgttgc gcggcatcca gcttcaaggc aaacagttgg
     2761 atgcgctgag tcagtttctc gtcggaacca caagcgacat cttttccatc atcgaaaaat
     2821 cacctgccgt cggcaagagc ttgggagaga tcaacctgcc ttcacgaagc ggagtatccg
     2881 tcattgctgt ggttagggag ggtaagtcct accccaacgt tgataacgac tttaaactgg
     2941 cggtcggcga catgcttgtt ctcttgggcg ggcacaaggc cttggatgat gcggcgcaga
     3001 ttctgaatcc gagcggcgac aacaatgcat cctgaccttc ctgtaaggat tctttacaga
     3061 tcatttttga gcccgcctga aaaaaatttc ttcgcggcaa atttttcttg ctttttattt
     3121 cctttcgtgt agccttttgt ttcttgattc ttgtaatcgg cattgagaag gaaagtgatc
     3181 gtccaggatc tttaacgctt ccgaatccca aactgaaacc acattcatgg caagcataga
     3241 cgacaatctc cgtaaagttc caccccagaa tctcgaagcc gaatcctctg tattgggcgg
     3301 aatcctgctt gaaaacgacg ccgttaatat agttttggaa ttgctccgcc cggaagattt
     3361 ctatcgcgaa tctcaccgca agattttccg cgccatgatc gagctcaccg atcggagcga
     3421 accagtggat ctcatcacct tgagcgagtg tctcaaaaac cggggcgaaa ttgacgcggt
     3481 gggaggcagt gcctacttgg cttctctggc tgattttgta ccgacagcgg ccaacatctc
     3541 ttactatgcg cgtatcgtgc gggaaaagtc gatcttgcgc agtctcatca gcacggcaac
     3601 agagattgct acgcggggtt atgaagagca gggcaacgtc gaagagtttt tagacgcggc
     3661 ggagaaagtg atcttcgata tatccgaaaa gaaggtcaaa gcgtccttca tcgccgtcgg
     3721 ggatatgatc aaagacacgc tcaaaaccgt cgaaaggctg tatgagcgca aggagatggt
     3781 caccggggtg cccacaggct acgaggattt ggacaaactt acggccgggc ttcagccatc
     3841 cgacttggtc atagtcgccg gtcgacccag catgggcaag accgctttca gcctcaatat
     3901 cgccacaaac gcagcgtttg cgggggtggg agtggcggtt ttttcactcg aaatggccaa
     3961 ggagcagctg gttctccgca tgctttgttc cgaagcccat gtcaacaatt ccaaagttcg
     4021 ctccggttat ttggcagaac gagattttcc gaaattggcg aacgctgcgg ggaggctcca
     4081 cgaggcgccg atctatatcg atgatactcc ggcgatttca gttcttgagc tgcgcgccaa
     4141 agcgcggcgc ttggtgcgtg atcggacgaa gaaagtcggg ttgatcgtca ttgattacct
     4201 gcagctgatg cgaggcatgg gagccgcgtc aaaccgcgaa caggaaatct cagaaatctc
     4261 gcgctcttta aaggccttgg ccaaagaatt gaccgttccg gtcatcgcca tctcgcagct
     4321 caaccggcgg gtggaggatc gcggcgaccg gcggccaatg atgtccgacc tgcgtgaatc
     4381 gggcgccatc gaacaggacg ccgacgtcat catgtttatt tatcgggacg aggtttacaa
     4441 tcagaactcc gagaaaaaag gtatcgcgga aattattgtc gccaagcaac gcaacggacc
     4501 catcgatacg gtggagctga cctttctcaa cgagtttatg cgctttgaga attatacgcc
     4561 gcgtgacgaa ttcgactatg aagaggcgga cgaagcatag agtttgattc ctgttgctcc
     4621 ggagtcaggt cgccgcactt tctcggctcc gcacaaagtt caccattttt acgctcatca
     4681 cgacttcgcg attctgattc agcacttcga caaaagagcg aacgatgccg cgatccggct
     4741 tggattcgga acggcgggct tccaataccg ttacgcgaat cgacaggtca tcgccgggga
     4801 agacgggctt atcccatttg agctcatcgc cgcctgggga accgaggttc gctaccttgg
     4861 atagatagtg atcggtgtaa agccgcatca tgagagcggc cgtatgccaa ccactcgcaa
     4921 tcaggcgacc gtagatactt ttcttcgcta gctccgggtc cgtatggtac gacaacggga
     4981 caaagcgctt gccgaagtcg atgacttccc gctcctcgac agcgatggag ccgaactcgt
     5041 gcaccgcacc ggcgacgtag tcttcaaaat aacggttgtc gggtgaagtg gtaaaggtcg
     5101 atccgttcat agtgtccgtc tggcggttcg cctaagccga ctggttagaa tgttggagaa
     5161 aatttacccg aagtttgggc aggctctcac cttaaatccc gtaaaattta gcggcgttgt
     5221 cgcccaggag acgccgcttg acgacgtcgt tgaggtcggc ccgctcccgg atctcttgga
     5281 cggaaccctc gcgcgcctca ccgtgaggca tgtcggccga gatcatgatc cggt
//
LOCUS       DASSWZ010000118         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1319597, whole genome shotgun sequence.
ACCESSION   DASSWZ010000118 DASSWZ010000000
VERSION     DASSWZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319597"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VFN58_02980"
     CDS             complement(<1..151)
                     /locus_tag="VFN58_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916987.1"
                     /translation="MSNVTGTVPSNAHVQKWVKDVAALCQPDEVFWCDGSDEEKDELT
                     RVAFKC"
     gene            complement(255..1172)
                     /gene="truB"
                     /locus_tag="VFN58_02985"
     CDS             complement(255..1172)
                     /gene="truB"
                     /locus_tag="VFN58_02985"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET8916988.1"
                     /translation="MTKSDLVVDSGLLLIDKPEGPSSAQVVHRVKVLLRARKVGHLGT
                     LDPFASGLLLLGINEGTKVADIFLSAPKSYHGIMVLGVDTDSQDATGTIVQVRAVPAI
                     GKSELRDLERHFSGDQLQVPPMFSALKKDGVPLYRLARQGKEVRREPRSIRVEKLRLH
                     KLGNDEIEIELTCSRGTYVRTLAADMGKSLGCGAHLKSLRRIACGHLTLDQAVTLDDL
                     ERRDPSMTVPLLSLEKALGHLRAITWENQLLSRLRLGQQEILRQLDKPVEGEKLARIL
                     DPRGGLVALVEWIDDVPQSRWRLSRIFKV"
     gene            complement(1215..1556)
                     /gene="rbfA"
                     /locus_tag="VFN58_02990"
     CDS             complement(1215..1556)
                     /gene="rbfA"
                     /locus_tag="VFN58_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET8916989.1"
                     /translation="MDYQRSDRVGDLLVELIAELLRKEIRDPRVGPVTITAAKVSKDL
                     RHARIYFNLFGGRENKKEVLAGLESATGFIRSKIGKQLKLRFVPTIEFFYDETQDEAQ
                     RIEDLLKQVKQ"
     gene            complement(1576..1863)
                     /locus_tag="VFN58_02995"
     CDS             complement(1576..1863)
                     /locus_tag="VFN58_02995"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET8916990.1"
                     /translation="MVVGVLKLNLLLPENHSLKGKRGVLKRIQAKVANQFNISVTECG
                     DQDLWQSAVLGFGVVGTSQAILEATLQNIVEFVDAMGLAEVGRAETEFYHC"
     gene            complement(1903..>2551)
                     /locus_tag="VFN58_03000"
     CDS             complement(1903..>2551)
                     /locus_tag="VFN58_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET8916991.1"
                     /translation="RVVLKADVQGSVEALTEALSRLSSDEVKFRVIHGSVGGITESDI
                     LLAAASNAIVIGFNVRPESKGAALAAKEGVDVRLYTIIYEAVADVRAAMEGLLEPTYR
                     EQILGRVEVRQIFNISGAGAVAGCYVAEGKVQRGSLVRLLRDQVVVHEGKLGSLKRFK
                     DDVREVAAGYECGLAIEGFQDVKQGDMVEAYEKIPVIRRIAPSAGGREASHVSRG"
BASE COUNT          584 a          768 c          615 g          584 t
ORIGIN      
        1 cgcatttaaa agcaactcgg gtcagctcgt ccttttcctc gtccgaacca tcgcaccaaa
       61 aaacttcgtc aggttggcat agcgcagcca catctttcac ccatttttgg acatgggcat
      121 tggagggtac cgtacctgtt acattactca tgacgttaat ttctcccctt gcaggtttta
      181 ctccagactt ttgaaactat ccgattgaga acggggatgt caaccggacc aatcatcgta
      241 aaacgcaccc aaaactatac cttgaatatt cggctaagcc gccagcggct ctgcgggaca
      301 tcgtcaatcc actccaccaa agcaaccagc cctccgcgag gatcgaggat acgagccagc
      361 ttctcgccct cgaccggctt gtctagttgc ctcaggatct cttgctgacc aagtcgcagg
      421 cgagacagca gttggttttc ccaggtaatg gcccgaagat gacctagggc tttttccaag
      481 gacaggagag gaaccgtcat gctcggatcg cgccgctcaa gatcgtccaa agtcaccgcc
      541 tgatctagcg tcagatgacc gcatgcgatg cggcgaaggc tctttagatg tgcgccgcaa
      601 cccagcgatt tccccatgtc ggccgccaga gtccgaacat aagttccacg agaacaggtc
      661 agctcaattt caatctcgtc gttgcccaac ttgtgcaatc gcagtttttc gactctgatg
      721 gaccgcggct cccgacgcac ttctttcccc tggcgtgcca gcctgtagag aggcacaccg
      781 tcctttttta acgctgaaaa catcggcggc acctggagct gatcaccgga gaaatgccgt
      841 tcgagatctc tgagctcgct tttcccaatg gccggcaccg cgcgaacttg gacaatagtg
      901 ccggtggcgt cttgggaatc ggtgtccact cctaagacca tgatgccgtg ataactctta
      961 ggggcgctca gaaatatgtc agcaaccttg gttccttcat tgattcccaa aagcaacaat
     1021 cctgaagcaa atgggtccaa ggtgcctaga tgaccgacct tcctcgctcg caacagaact
     1081 ttgacccgat gcacgacctg ggccgaggag ggtccttctg gcttgtcgat caaaaggaga
     1141 ccgctatcca ccacgagatc gctcttggtc acgacgattg cacggaacga aatgataaat
     1201 ttatgaagga agcgctactg cttgacctgt ttcagcagat cttcgatccg ctgggcttca
     1261 tcttgagtct catcgtagaa gaactcgatg gttggaacaa atcgaagctt cagttgtttg
     1321 ccgatctttg aacgaatgaa tcccgtggca ctttccaagc ccgccagcac ttctttctta
     1381 ttctcccggc caccgaaaag gttaaaatag atccgcgcat gacgcaggtc tttgctgacc
     1441 ttggcagccg tgatcgtaac cgggccgacg cgagggtcgc ggatttcctt tctcagtaat
     1501 tccgcgatca actccaccag caaatctccc acacgatctg aacgttggta atccatgagc
     1561 catgactaaa cgaagtcagc aatgatagaa ctcggtttcg gcccttccca cttcggctaa
     1621 ccccatagcg tcgacaaatt ccacgatgtt ctgaagagtc gcttctagga tcgcctgact
     1681 cgtgccgacg acgccgaacc ctaggaccgc actttgccag agatcctgat caccgcattc
     1741 agtaacggag atgttaaatt gatttgcgac tttcgcctgg attcttttca gcacccctcg
     1801 cttccccttc aaggagtgat tctcggggag cagaaggttg agcttgagaa cccccacgac
     1861 catcgaagcg gcggacgatt cgtaatgacg tcggctgatc gcttatcccc tgctcacgtg
     1921 cgatgcttct cgtcccccgg cgctgggcgc gatacgtctt atcaccggga ttttctcgta
     1981 ggcttcgacc atatccccct gcttgacatc ctgaaagcct tcgatggcga gaccgcattc
     2041 gtaaccggcg gcgacctcgc ggacgtcgtc cttgaaacgt ttcaggctgc ccagtttgcc
     2101 ttcgtgcacg accacctgat ctctcagcaa ccgcactaaa ctaccgcgct ggacttttcc
     2161 ctcagcgaca tagcagccgg caaccgcacc cgcgccggag atattgaaaa tctggcgaac
     2221 ttcaacgcgt cccagaattt gctcgcggta agtcggctcc aacaatcctt ccattgccgc
     2281 cctgacatcg gccaccgctt cgtagatgat agtgtacaaa cgaacgtcaa cgccttcttt
     2341 agcagccaag gcagcaccct tggattccgg ccgcacattg aagccaatta cgatagcgtt
     2401 ggaggccgcg gccagtagga tatcactctc ggtaattccg ccgaccgaac cgtggatcac
     2461 gcggaactta acctcgtcac tggagaggcg gcttaaagct tccgtcagag cttccacaga
     2521 gccctggaca tcagctttga ggacgacccg g
//
LOCUS       DASSWZ010000119         5673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_980131, whole genome shotgun sequence.
ACCESSION   DASSWZ010000119 DASSWZ010000000
VERSION     DASSWZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980131"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFN58_03005"
     CDS             complement(<1..209)
                     /locus_tag="VFN58_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916992.1"
                     /translation="MRLLIYGFGRYRHFQYNVTEKILRRLPRRNGLKKIVFPVRFHRG
                     QFLNAIKKHRPDLVLGLGQCAHGQR"
     gene            complement(325..567)
                     /locus_tag="VFN58_03010"
     CDS             complement(325..567)
                     /locus_tag="VFN58_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916993.1"
                     /translation="MANECDVFVTGGTGYMGSRLISTLVGRGRNFSRGYTRRKIRVTI
                     VPSARPDSVTKRWMIQYNRDAKRTRATNRRIMGYRS"
     gene            851..1903
                     /locus_tag="VFN58_03015"
     CDS             851..1903
                     /locus_tag="VFN58_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8916994.1"
                     /translation="MKTPRMALVFYLSLILVLLFFAPPIGAQGEPFYKGKQIRIIVGL
                     SSGGGYDRAARMLARHIVKYIPGNPDVVVQNMPGASSITAANYVWGVAKPDGLTILAP
                     HNNVYLGQLSGQKEVQFDLPKFQWIGSLENDDMMIFGRADASFKSMGDIVRAKEPPKC
                     GSTGVGSSDYVMSKILEETIGAKVTAVTGYPGSSEIAIALERGEVACMGLTISTYFGR
                     EPFLTWLKTKFVRFLAQSGRKRQPRVEEAPTIYELMDEYKTSATKRRVAEAMLQGGEW
                     ARPYMVAPGTPADRVAILRAAFDKVVKDPELLAEAQKLRMDVTPMRGEQLQTIAKEVM
                     VQPPEVIEQIKKLFVQ"
     gene            complement(2063..2656)
                     /locus_tag="VFN58_03020"
     CDS             complement(2063..2656)
                     /locus_tag="VFN58_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET8916995.1"
                     /translation="MSSLKGNICVFTGSRQGIRPDYADAARQIGRELVERGYGLVYGG
                     GNVGLMTVVADTVLECHGHVTGVIPERLVEKEVVHRGLSELRIVQSMHERKATMAELS
                     DGFVALPGGIGTMEEFFEVLSWAQLGMHNKPCGLLNVGGYYQHIVQFLDYAVEQDFLK
                     PKHRALLIVEAEPGWLLDRFEQFIATHAAKRFDRTKT"
     gene            complement(2723..3721)
                     /locus_tag="VFN58_03025"
     CDS             complement(2723..3721)
                     /locus_tag="VFN58_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8916996.1"
                     /translation="MPRMMLRFLTGVTILFAISSMAKAQSPLEKLRLTYSAIGGSQAS
                     VWVPYEAGIFRKHGLDVELLYVGGGGRAAQVVQSGEVPIGIFTGGAVINSNLAGGDLV
                     MIASSMNVMTFAVMARPEIRSVEELKGKKIGISRFGSATDFGLRYAEGKWPIKRQRDF
                     AVIQVGGMPEQLTALRTKALDAAVLNAELAIVARKEGFRELVDLAKVGLNFPTSSIVT
                     TRSFIKRNENTVRRFIRGYVEGVHYGKTQRAFGVQVLKKYLRNEDTAFVNELYDLYIL
                     QNIPQIPRPSPEALKTVLDQMAESDSRVANLRPEQFIDARFFQELEKEGFLQRLWK"
     gene            complement(3845..4237)
                     /locus_tag="VFN58_03030"
     CDS             complement(3845..4237)
                     /locus_tag="VFN58_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916997.1"
                     /translation="MISQWFVYLGVVVLLMTLGRVAWRRGSKPVDWALIVAFIRLDFP
                     PDQRDLAQKVAGGLAEIVGLKIKQLRPEHTIKQIADWANDPVSVAVLIKIFHAAFQIT
                     CEGSTTFRTLVEMIAAQQIKGAERLSRT"
     gene            complement(4237..4422)
                     /locus_tag="VFN58_03035"
     CDS             complement(4237..4422)
                     /locus_tag="VFN58_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8916998.1"
                     /translation="MSWKVTSNFIANDLEITIHFWFVPNSHDLSVLDNAGNLAPALGE
                     FDAVKKFFVSATGDLAF"
     gene            complement(4419..4886)
                     /locus_tag="VFN58_03040"
     CDS             complement(4419..4886)
                     /locus_tag="VFN58_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET8916999.1"
                     /translation="MDIILEEMFSGLPDLRQMVSLVSRLLTAMILGALVGAQRESIGK
                     PAGLRTHMLVAMGSALFVLAPLEAGMELDGISRVIQGIVTGIGFIGAGAILKLQEKRE
                     IEGLTTAAGIWMTAAIGIAAGLGRWGLALVSTILTWITLSVIGKIETWMNQKP"
     gene            complement(4904..>5673)
                     /locus_tag="VFN58_03045"
     CDS             complement(4904..>5673)
                     /locus_tag="VFN58_03045"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HET8917000.1"
                     /translation="VVIWTDACAPARNLTLNVMKIRKRQVPVFVALFLFNACAMQQTG
                     RVAQDQPQRHVPLFSAERAIQDEWQEILIRGKTEYRVTASTGRVAIRAIGQNSASGLL
                     RRVQVDPVNCPKIEWSWRVDQLQETADIRVKEKEDVAASIYLMFGDPGLLLTPKQVPS
                     LRYVWTNQRMDVESIVDSPYLPGTVKSLVVRSGSRQPEWFIESRNILKDFERAFGYTP
                     KEAIEAIALFTDNDQTKEPVLAYYEWGRLECRTDRES"
BASE COUNT         1291 a         1608 c         1461 g         1313 t
ORIGIN      
        1 aaacgttgtc cgtgggcgca ctgtcccagg ccgaggacga gatcgggtct gtgctttttg
       61 atggcgttga gaaattgccc cctgtgaaac ctaaccggga agacgatttt tttgagcccg
      121 tttcgtcggg gtaaacgccg gagaattttt tcagtaacgt tatattgaaa atgccggtag
      181 cggccaaagc cgtagatgag gaggcgcatt gaaatatcac tcaatcagcg actcggcatg
      241 ccgggcgtgc acaggctcat ccgctctgca cgagttccgg gtttcaggtt tcgggtgacg
      301 ccgaatagtt cgatccgatc taagtcaact tcggtagccc attattctgc ggttcgtagc
      361 tcgggtccgt tttgcgtctc tgttgtactg aatcatccac cgcttcgtta ccgaatcggg
      421 tcgcgccgat ggcacgatag tgacgcggat ttttcttcgc gtatagcccc ttgagaaatt
      481 tctcccgcgc cccaccagtg tcgagatcag cctactgccc atgtagccgg ttccgccggt
      541 tacaaagaca tcacattcgt tggccacgaa ggtcctctat cgacaaaacg aggataccgg
      601 cccgattgat aggtggcaag caatggcgtc ccctttttgt ctcgggtaac aatcattgct
      661 cttggcatct gtgatccgac aatcttgaca agtcagcatc cggcgatata ttgccgtgac
      721 ccagcgagat acggactcgg gctgggccat gctcatccga tatctcttgt tctagatgac
      781 ggggctgagt cctacggctg catcactaaa attttgcaac ggtcgatttg attgccgtaa
      841 agaggttatg atgaaaactc ccagaatggc tttggttttt tatttgtcgc ttatcctagt
      901 tttgttgttt ttcgcacctc cgatcggagc gcaaggcgag cccttctaca agggtaagca
      961 gatccgtatc atcgtcgggc ttagcagcgg tggaggatat gaccgggcgg cacgaatgct
     1021 ggccaggcat atcgtcaaat acataccggg gaacccggac gttgtcgtcc agaacatgcc
     1081 gggggcaagc tcgatcacgg ctgcgaacta cgtctgggga gttgccaagc ccgatgggtt
     1141 gacgatcctc gcgccgcaca acaacgtata tctgggccag ctttcgggac aaaaggaagt
     1201 ccagtttgac ctgccaaaat tccaatggat cggctctctc gaaaacgacg acatgatgat
     1261 tttcgggcgt gccgacgctt ctttcaaatc catgggcgat atcgttcgcg ccaaagagcc
     1321 accaaagtgc ggctccaccg gtgtcggaag ttcagactac gtgatgtcga agatcttgga
     1381 agagactatc ggagccaagg tcaccgccgt aaccggctac ccaggcagca gcgaaattgc
     1441 catcgccctc gagcgaggtg aggtcgcatg tatggggctc acgatttcga cgtacttcgg
     1501 gcgtgagccg tttttgacct ggctcaaaac caaattcgtc cgcttcctgg cgcagagcgg
     1561 ccgtaaacga cagccgcggg tcgaagaagc gccgacgatt tatgagctga tggatgaata
     1621 caaaacatct gcgaccaagc gccgcgtcgc cgaggctatg ctccagggcg gcgaatgggc
     1681 tcgtccctat atggtggctc cgggaacacc cgcggaccgc gtcgccatcc tccgcgccgc
     1741 cttcgacaag gtcgtcaagg atcccgaact tctggctgag gcgcagaagc ttcgcatgga
     1801 cgtcactcct atgcggggcg agcaactgca aacgatagcg aaggaagtga tggtccaacc
     1861 gcccgaagtc atcgagcaga tcaaaaaact gttcgttcaa taaggattgc aagacgcggc
     1921 gggccagttg cacgtgccga cgatctcgtc agtttgctgg aaagttcagc agcaaataac
     1981 gtcagatcgc tcttcggcgc cgcccggaaa aaactccgcg agacggccca ctcgctgttt
     2041 ggtttatacg aggacaaaaa ccttacgtct tggtgcgatc aaaccgcttt gccgcatgcg
     2101 tcgcgatgaa ctgctcgaag cggtcaagca accagcccgg ttcggcttca acaatcagta
     2161 gcgcgcgatg ttttggtttg aggaaatcct gctcgactgc gtagtcgaga aactgaacga
     2221 tgtgctgata gtagccgccc acatttaaca agccgcaggg tttgttatgc atgccgagtt
     2281 gcgcccagct caggacttcg aaaaactcct ccattgttcc aatcccgccc ggcaaagcga
     2341 caaagccgtc ggacagctcg gccatcgtgg ctttccgctc gtgcatcgac tggacgatcc
     2401 gcaactccga gagtcctcgg tggacgactt ctttctccac caaccgttcc ggaatcacgc
     2461 cggtaacgtg gccgtgacat tccaacacag tatccgctac cacggtcatg agaccgacat
     2521 taccgcctcc gtacaccagt ccatagccgc gctcgacaag ctcgcggccg atttgccgtg
     2581 cagcgtccgc gtagtcaggt cgtatgccct gacgcgatcc ggtgaagaca cagatgtttc
     2641 ctttcaagct gctcatacca acctcagtga cgcctacaat tcatcaccct cagtcggcag
     2701 agccgaattg tctcggcgat cctcatttcc acaggcgttg aaggaagccc tccttttcca
     2761 attcctggaa aaagcgggca tcgataaact gttccgggcg aagattcgcc actcgggaat
     2821 ccgactcagc catctggtcc aagacggttt tcaaggcttc cggtgaaggt ctcgggattt
     2881 gcggaatgtt ctgcaggata taaagatcgt acagttcgtt gacgaacgca gtgtcctcgt
     2941 tccgtaaata ttttttcagc acctgaacgc cgaaggctcg ctgggtcttg ccgtagtgca
     3001 ctccttcgac atagccgcga ataaacctcc ggaccgtgtt ttcgttgcgc ttgatgaagg
     3061 agcgggttgt gacaatggag gacgtcggaa aattgagacc taccttggcc aagtcgacca
     3121 gctccctgaa tccttcctta cgcgcgacaa tagcaagctc cgcattgagc acagcagcgt
     3181 ccaacgcttt agttctgagc gccgtcaatt gctcgggcat gcctcctact tggatcacgg
     3241 caaagtcgcg ctgccgtttg atcggccatt ttccttcagc atagcgcagg ccaaaatcag
     3301 tggctgagcc gaagcgtgaa atcccgatct ttttcccttt cagctcctcg acgctgcgaa
     3361 tttctggacg agccattacg gcgaacgtca tcacgttcat cgaactggca atcatcacca
     3421 ggtcgccccc cgcaagatta gagttgatga cggcgccgcc ggtgaaaata ccgataggca
     3481 cctctcccga ttgcacaacc tgcgcggctc tgccgccgcc accgacatag agaagctcaa
     3541 cgtcaagacc gtgcttgcga aaaattcccg cttcataagg gacccacact gacgcctgtg
     3601 agccgccgat ggcgctgtac gtcaggcgca atttttccag gggcgattga gccttggcca
     3661 tcgaagagat ggcaaaaaga atggtaaccc cggtcagaaa gcgcagcatc atccttggca
     3721 taagcctccg ttccttcata ctcgacccaa ctgggtgcct actaaccgat ggcgacaaaa
     3781 aacggtcgcg aatagagtag ttgtgcgcaa aacccggcgt tctttgtggc tggtcaacat
     3841 cgcatcacgt tcgcgatagc cgttcggcgc ctttgatttg ttgtgctgca atcatttcca
     3901 ctagtgtgcg aaacgtggtg ctcccctcgc aggtaatttg gaacgccgcg tgaaatatct
     3961 tgatcaagac tgcgaccgac acgggatcgt tcgcccaatc ggcaatttgc ttgatcgtgt
     4021 gttcgggacg gagctgtttg atcttcaacc ctactatctc tgccagtcct ccagctacct
     4081 tctgcgccag gtctcgttgg tcagggggaa agtccagtcg aatgaatgca acgatcaagg
     4141 cccaatctac tggtttactg ccgcgacgcc acgcgacgcg ccccaaggtc atcaacaaaa
     4201 cgacaacgcc aagatagacg aaccattgcg aaatcattaa aacgccaagt ctccggtggc
     4261 agagacaaag aatttcttca ccgcgtcgaa ctcgcccagc gcaggggcca aattcccggc
     4321 attatccaga acagagaggt catggctgtt aggcacgaac cagaagtgta ttgtgatttc
     4381 caggtcgtta gcaataaaat ttgaagttac tttccaactc atggtttttg attcatccaa
     4441 gtttctattt ttcctatgac ggaaagcgtg atccaggtca gaatcgtact gaccaatgca
     4501 agtccccacc gtcctagacc tgcggcgatc ccaatcgcgg cggtcatcca tattcctgcc
     4561 gcggtcgtga gtccttcgat ctctctcttt tcttgcagct tgagaatagc gccggccccg
     4621 ataaatccga ttcctgtgac tattccttga atcacccgcg atattccatc caactccatt
     4681 ccggcttcta gaggagcgag aacaaatagc gcgctgccca tggccaccag catgtgtgtt
     4741 cttagccctg ccggcttgcc tatgctctct cgctgggcac ctacgagagc ccctaggatc
     4801 atggcggtca gcagtcgact gacgaggctg accatctgtc ggagatcagg caatcctgag
     4861 aacatttctt ccaaaattat gtccatcgtt gatcaatctt gtttcatgat tctcgatctg
     4921 ttctacactc caggcggccc cactcgtagt aggctaatac gggttccttc gtttggtcgt
     4981 tgtcggtgaa tagggcgata gcctcgatgg cttctttggg agtatagcca aaagcccgtt
     5041 caaaatcctt caaaatattt cgactttcta taaaccattc cggttgcctt gatccgcttc
     5101 gcactaccag gctcttcacc gtgcccggta agtaagggct gtcgacaatc gattcaacat
     5161 ccattcgctg gttcgtccaa acgtaccgga gggacggcac ctgtttcgga gtcagtagta
     5221 acccaggatc gccaaacatc agatagattg aggcggcgac gtcctctttc tctttgacac
     5281 gaatgtccgc ggtttcctga agctgatcga cccgccaact ccactcgatt ttcgggcaat
     5341 tcaccggatc cacttggaca cggcgcagca aaccggatgc ggaattttga ccgatagcac
     5401 ggatcgcgac tcttcccgtg ctggcagtga cgcggtattc cgttttgccc cgaatcagaa
     5461 tctcttgcca ctcgtcttgt atcgctcgct cggcagaaaa caaaggaacg tgtcgctgag
     5521 gttggtcctg ggcaacacga cccgtctgct gcatcgcaca ggcgttgaag aggaagagag
     5581 caacaaagac cggcacttgc cgctttcgta tcttcataac attcaatgtc aaatttcggg
     5641 caggcgcaca tgcatcagtc caaatcacga ccc
//
LOCUS       DASSWZ010000120         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1036898, whole genome shotgun sequence.
ACCESSION   DASSWZ010000120 DASSWZ010000000
VERSION     DASSWZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036898"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1359
                     /locus_tag="VFN58_03050"
     CDS             328..1359
                     /locus_tag="VFN58_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8917001.1"
                     /translation="MQRIIVGFFFLLTSVLPLQAQTPFYQGKSIKLVVGSPAGSNYDQ
                     YGRLIAPYLSKHIAGNPEVVVQNIGGAGSVIAANQVYRVATPDGLTLVSILPAIYLDQ
                     LVGRKEVQFDYAKFSWIGSTDRSNNLLIMRADTPYKTLRDVRDSTQVPKCAATGTGSI
                     AYYMPKLLEETVGTKFDVVTGYPGGPEMDLAVERNEAQCRAFTLTAFLSNREPYLSWR
                     KNRFVRVLVQTGSKRDSRLPEVPTLNELMDEYKTPESSRRLAKLVLASGDLGRPIMGP
                     PGVPADRVKILRQAFLKALSDPELLAIAEKQRLEIEPTSGEELEALAKEVMAQPPEII
                     ERMKMLLGK"
     gene            1435..1872
                     /locus_tag="VFN58_03055"
     CDS             1435..1872
                     /locus_tag="VFN58_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917002.1"
                     /translation="MGSIKVEGVTHWSIPVNDLEESEKFYGDLLGLKPLGRLGNGVMS
                     CYNVGDHNILLCEREKPVEKISDVEQRVHHSFTVSPESFIQACKVFHEKKIPIVELHY
                     RAKGYFTGRELYFHDPSGNRLELRDPTWKQGMPEPPYEEIVRS"
     gene            complement(1917..2621)
                     /locus_tag="VFN58_03060"
     CDS             complement(1917..2621)
                     /locus_tag="VFN58_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917003.1"
                     /translation="MQAIADYVGLVTEHFKLRLGSSLVEAYKIGSLAHGGFSEIYSDL
                     DVGLILSCQTPPADMDRMIAVAKALDDHYGGKLSIFWGNPEFSWGRLPALDRLDLLDH
                     GVPLLNNRRANFRRPTRDEIRQQLSQTIERTWKPNSDELSQLKKLEPKGHKPYIRAVL
                     YPARLIYSWDNLAMDSNDRAVAYLREVRPTGLDLLPIEKALACRQGECSAEEVFSLGA
                     DLSRQFESTVSYISTR"
     gene            2786..>3541
                     /locus_tag="VFN58_03065"
     CDS             2786..>3541
                     /locus_tag="VFN58_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8917004.1"
                     /translation="MATLITGLGLVGTSYAQLALKRGESIIFYDLAPRKDFLAHKLGT
                     ANVTVLQGDVRDLPTLVEAMQKHKVDKVIHTAGLIGGKVAQPLYSGLQINVMGTINVA
                     EAVRLTGVKRLVQISTMGTYDRRLEGDSPIDENFHRGPGEAYGNSKVAKELMVEAYQR
                     LYGFELIIVRLANVYGVGHFAGGSAGGEMVQTLLQTGIKGGVAKINQEQTRDFEYIYY
                     KDLGRALDKATTVALSGPTTLNIGTGVIVTFDDL"
BASE COUNT          833 a          941 c          960 g          807 t
ORIGIN      
        1 cgtacacaac cttcggttag attttcacat gactcaacga accccacttc cggttagatt
       61 ttcagatggc ttgacaggct taggggcggt ctacagaagg attggtgatc ggccttgcca
      121 ccggtttcga ggagaaggga gtaaacgcca gagttgatct acaccttttt ctccttgact
      181 cctctcttcc gtctgcccta ttttacgcgc atctaatttc ggctgggtct gccgttccgg
      241 tcacatctcc gattcgttgt ccatgagccc ggattgggtc gtatccagcc ggttttttgt
      301 tttgcaatta gcggaaagga aagtgcgatg cagagaataa tcgttggttt tttctttttg
      361 ttgacatcgg ttctccctct acaagcgcag acccctttct accaaggcaa aagcataaag
      421 ctcgtggtgg gaagtcccgc ggggtctaat tatgaccagt atggccgcct gatcgctcct
      481 tatctgagca agcacattgc cggaaatccg gaggttgtgg tgcaaaacat aggcggcgcc
      541 ggttctgtta tcgcagccaa tcaggtttat cgtgtagcca cgcctgatgg cttgactctc
      601 gtgtcgattc ttccagcgat ctacctcgat cagctcgtgg gacgtaaaga ggtccaattt
      661 gattacgcca aatttagctg gatcggcagc accgaccgct cgaacaattt gctcatcatg
      721 cgggccgata cgccttacaa gacactgcgc gatgtgcgcg actccaccca ggtcccgaaa
      781 tgcgcagcca ccggcaccgg ctccatcgcc tactacatgc ccaagctttt ggaggagacg
      841 gtgggaacca aatttgatgt ggtaacgggc tatccgggcg gaccggagat ggatctggcg
      901 gtggaaagaa acgaagccca gtgccgcgcc tttaccttaa ccgctttttt gagcaaccgt
      961 gagccttatc tcagttggcg taaaaacagg tttgttcggg tcctagttca gaccgggtca
     1021 aagcgggatt cgcggctgcc cgaggttccc accctcaatg agctgatgga tgaatacaaa
     1081 acccccgagt cgtctcgtcg tctggccaaa ttggtcctgg cttccggcga cttgggccgg
     1141 ccgattatgg gaccgccggg ggtgcctgcg gaccgtgtca agattttgcg ccaggctttc
     1201 ttgaaggcac tgagtgatcc cgaacttcta gcgatcgcgg aaaagcaaag actggaaata
     1261 gagcccacca gcggtgagga gttggaggcc ttggccaaag aagtaatggc gcaaccgccg
     1321 gaaattatcg aacggatgaa gatgcttctc ggcaaataaa gctgctgagc gtttgccaac
     1381 tccggaaggg acagactata agttaaagta aaccagatac gcatggaggt ttctatggga
     1441 tcaatcaaag ttgaaggcgt cacgcactgg tcgattccgg tgaacgacct ggaagagtcg
     1501 gagaagttct atggcgatct gctcggcctt aaaccgttag gtcggcttgg caatggggtc
     1561 atgtcctgct acaacgttgg cgaccacaac atcctgcttt gcgagcgcga gaagccggtg
     1621 gaaaaaatct ccgatgtcga gcagcgggtc catcattcct tcaccgtgag cccggagtca
     1681 ttcatacaag cctgcaaggt tttccacgaa aaaaaaatcc ccatcgtcga gctgcattat
     1741 cgggcaaagg gttattttac gggccgcgag ctttatttcc atgatccgag cggtaaccgg
     1801 ctcgagttgc gcgatccgac ctggaaacaa ggaatgcccg agcccccata cgaggaaatc
     1861 gtccgctcct gaggtagccc agcttcaacc gcttggaaat gctgcagtcc gctggctcat
     1921 cgcgtggaaa tgtaggagac cgtgctctcg aattgccggc taaggtcagc tcctagagaa
     1981 aacacttctt ctgcggaaca ttcgccttgg cggcaagcta gagccttttc aatcggcaac
     2041 aaatcgagcc ctgtcggccg gacctcacgg agatatgcca ccgcccggtc gttggaatcc
     2101 atggcgagat tatcccaact ataaatcaaa cgcgccggat aaaggacagc tcgaatgtag
     2161 ggcttatggc cttttggctc cagtttctta agttggctaa gctcgtcgct gttaggcttc
     2221 caggttcttt cgatcgtttg actcaattgt tgacgaatct cgtctctggt cggtcggcga
     2281 aaattggccc ggcggttatt cagcaacggc acgccgtggt ccaggagatc cagccgatcc
     2341 agtgctggca accgtcccca gctgaattca ggattgcccc aaaaaatgga gagctttcca
     2401 ccgtagtgat catccaaagc tttagccaca gcgatcatcc gatccatatc agccggagga
     2461 gtctggcagc ttagaatcaa tccgacgtca aggtcgctgt aaatctcgct gaatccccca
     2521 tgggccaggc tgccgatctt gtaagcttca accaatgagg acccgagtct taatttgaaa
     2581 tgttcagtaa caaggccgac ataatcagct atggcttgca tcgggaacgg ctcccttccg
     2641 ctctttgccg cttttatagg ttagcaccgt tgaagatgca acgctgacct tgaaatcgct
     2701 gcttcctaca atcacacagc tagacatttg ccagcccttg aagtatggga agataaatca
     2761 aagactcaaa aagaaaggtg tcatcatggc aacgctgata accggtttgg gtttagtagg
     2821 aacatcctat gcgcagctcg cgctaaagcg cggtgagagc atcatcttct acgatctggc
     2881 gccccggaaa gattttttgg cgcacaagtt gggcacagca aacgtcacgg tgctccaagg
     2941 agatgtgcgg gatcttccga cgctggtgga agcgatgcag aaacacaagg tcgacaaagt
     3001 cattcatacg gcgggcttga tcggcggcaa agtggcccag ccgctctaca gcggcttgca
     3061 gatcaatgtc atgggaacca tcaacgtcgc tgaggcggtg cggctcacgg gagtcaagcg
     3121 gctcgtgcaa atcagtacca tgggaaccta tgaccgccgg ctggagggag acagccccat
     3181 cgatgagaat tttcaccgtg ggcctggcga agcctacggc aactccaagg tggccaagga
     3241 gctgatggtc gaagcctacc agcgtcttta cggatttgaa ttgatcattg tccgtctcgc
     3301 caacgtgtac ggcgtcggcc actttgccgg cggttccgcc ggcggcgaaa tggtccaaac
     3361 cttgctccag accggaatca agggcggcgt ggccaagata aaccaggagc agacccgaga
     3421 ttttgaatac atttattaca aggatctcgg ccgcgctctt gataaggcca ccaccgtcgc
     3481 attgtccggg ccaaccacat tgaacatcgg tacgggcgtg atcgtgacat tcgacgatct
     3541 c
//
LOCUS       DASSWZ010000121         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_909402, whole genome shotgun sequence.
ACCESSION   DASSWZ010000121 DASSWZ010000000
VERSION     DASSWZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909402"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2155)
                     /locus_tag="VFN58_03070"
     CDS             complement(<1..2155)
                     /locus_tag="VFN58_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917005.1"
                     /translation="MRQFKLFSLWFFLSLIFVSLSVLFPLIAGAAIVGFYAGAFDPPT
                     QAELGIVRCALGDASSHKECQEIGKAISRVVVVVNQAGDADPLASTRERLLMVKKGLQ
                     PYADRVELVAGDGEEKTRSLLGDRNVEQLIRFIDEDSYKVARSAPVNQDSRLLWVVVP
                     LKNQGSSSPAIDTSANVKLLSGIRELKGESSAAVQKAIEAGGTMGGLFDPAVKEVIER
                     LGLYQPVSEDLAALQKSLFEESWKGFLKDLKSACPSISNQKECADLGSAWDSISIATD
                     DSMERTDSKKSSANVLIYKTAQSGDRWAEKFTDTALKPLQGSARYAQFKPVAEDMSSK
                     IYQGYPDDKLFHLRKVFVQKQRPPMEPLKVSQKPASCSPPRGPYHMDIVDYTSDRFPI
                     AFAAFLREQFQKRTIAPTELYVHNQAVEAAYEFHRRDRFSTFYFLQTRRGQLHRNIYL
                     AVRSNPRAYRVVFSNVRGRDQEADVFCQIYHSGTFSSFHWVQANPGLRLFVFNPRGSA
                     LRLNDQDWLLFGFKGKWTKILLEQGWQLHPLVKEGLDIGLFTHPTIKQRLVVARNVYG
                     SDAEIILDTFYKKGVRQVVYLGTSGAIPNYPVGAVTIPNQVIDSNKSVPFQRNLAQAY
                     VADLSNRVTVHGGTKHGWVQTVYQETEALLLDWKSKSVDAMDVEGLYLARFAGSHDNL
                     KMSAFFVISDQTLGDSTIGEDESHLNIIDESVYKLV"
     gene            complement(2392..>4080)
                     /locus_tag="VFN58_03075"
     CDS             complement(2392..>4080)
                     /locus_tag="VFN58_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HET8917006.1"
                     /translation="KGPLIAAFEILEEALKRVMKFIVRLTPIGIFAIAASTAGTMNVD
                     QLSGLQVYIVTYAVAALLMTFGVLPALIMLLTPFGYREIVRATKEALLMAFATDNLLV
                     VIPMLSDHAKELLRQGMGTSAESESSVDVIVPVSFNFPNVGRLLQLIFILFAAWFTTS
                     AISLAGYFNLTLSGLLSLFGKPVAAMPFLLDLLRLPADMMQLYLASGIFVAQLGTLAS
                     AMHLFTLGVLGGFAMAGALRFQWKRFVPVTALTVGVVAAAVVGTRAYFSFAVENAYDK
                     AKIMALMQPLFPAVPAKIYKSAPPPGPVESSQPTMKRIRSRGAIRVGYIGDVRPFSFF
                     NAQGELVGLDAEMAHILARSLGVSLEFVPVDHSKMFEQLNGGYCDIIMSGIVVTPERA
                     EVVRLSAPYLEMTLAFVVQDYRRGEFGSREALQKLKAPKLAVLDVPYFISFVRELLPK
                     AQLVTLNSPSEFFESKGKTLDGFIYTAEEGSAWSLLYPEYTVVVPPPPISIPLSYPMA
                     RADQELVDYVNVLIDLKKSDGTLKKLYDYWVLGRFAANKQPRWSVIRNVLHWVD"
BASE COUNT         1031 a         1083 c         1035 g          931 t
ORIGIN      
        1 ccaccagctt atacacggat tcatcaatga tgttcaggtg agattcgtct tcgccgatcg
       61 tactgtcacc tagggtttga tcagagatga caaaaaacgc cgacattttg agattatcat
      121 ggcttccggc aaaccgcgct agatagaggc cttctacatc cattgcgtca accgattttg
      181 acttccaatc caacaacaaa gcctccgttt cctgatagac ggtctgtacc cagccgtgct
      241 ttgttccgcc gtggacggta acccgatttg acagatctgc gacataagcc tgcgctaagt
      301 ttctctgaaa cggtacagac ttgttgctat cgataacctg attaggaatc gtcacagccc
      361 caaccggata attgggtatc gcccctgagg ttcccaaata gacaacctga cgaacgccct
      421 ttttatagaa cgtatccaaa atgatttcgg catcgctgcc atagacgttc cgcgcaacca
      481 ccagtcgctg cttgatcgtc gggtgcgtaa agagaccaat gtccaaccct tcttttacga
      541 gaggatggag ctgccaacct tgctccagca gaatcttggt ccattttcct ttgaagccga
      601 aaagcaacca atcctgatcg ttgagtctta aagcgctccc ccttggattg aagacaaaca
      661 aacgcagtcc aggattggcc tgtacccaat ggaaactgga aaaagtgccg gaatgataaa
      721 tttggcaaaa aacatcggct tcctggtcgc ggccccgaac gttgctgaaa acgacccgat
      781 aggcgcgggg gttcgacctg actgcaagat agatattgcg atggagttga ccgcgccgcg
      841 tttgtaaaaa atagaaggtg ctaaatcggt ctcgtcgatg aaattcataa gccgcttcga
      901 cggcctggtt gtgcacataa agctctgtgg gagctatagt gcgcttctgg aattgttctc
      961 ttaggaaagc cgcaaaagca atcgggaaac ggtccgaagt gtagtcgacg atgtccatat
     1021 gatacgggcc tcgaggcggg gaacaactcg caggcttttg gctaactttg agaggctcca
     1081 tcggtggccg ttgcttttga acgaagacct ttctcagatg aaaaagttta tcgtcgggat
     1141 agccttggta gatcttagaa ctcatatcct ctgcgacggg tttgaactga gcgtagcggg
     1201 cggaaccctg taaaggtttt agggcagtat ccgtgaattt ctctgcccaa cgatccccgg
     1261 attgcgccgt tttgtaaatc agaacattag cagaggactt cttgctgtcc gttcgctcca
     1321 tcgagtcatc ggttgctata gaaatgctgt cccaggccga tcctagatcc gcgcactctt
     1381 tctgatttga tatactgggg cacgcggatt tgaggtcctt taagaaaccc ttccagcttt
     1441 cttcgaacaa tgacttttgc agcgctgcga gatcttcgct caccggctgg tacaagccca
     1501 atctttctat gacctctttc accgcgggat cgaaaaggcc tcccattgtt ccacccgctt
     1561 caatggcctt ctgaaccgct gccgaagatt cgcccttcaa ctccctgatc ccagaaagaa
     1621 gttttacgtt ggcagaagta tcaatggcgg gagatgagct accctgattc ttcagaggaa
     1681 ctacgaccca taggagtcgt gaatcttgat taaccggagc agatctcgcg accttgtaag
     1741 aatcttcatc gatgaaccgg atgagttgtt caacgtttcg atcaccgagc agggatcgcg
     1801 ttttttcctc gccgtctccg gctacaagct ccacccgatc ggcgtatggt tgcagccctt
     1861 tcttcaccat caataagcgc tctcgagtag aggccagagg atctgcgtca ccggcttgat
     1921 tcaccacaac cactacacgg gaaattgctt tgccgatttc ctggcattcc ttgtgagaac
     1981 tggcatcccc taaagcgcaa cgaacaattc ccagctccgc ttgggtcggg ggatcaaaag
     2041 cgccggcata aaaacccaca atagcggccc cggcgataag cggaaacaaa accgacaaac
     2101 tcacaaagat gaggctgaga aaaaaccaaa ggctgaaaag cttaaactgt ctcatggttt
     2161 ttatcctccc ttcccgactg tgtgaaaact cattcaaagc cgctcgacga ggctcagggc
     2221 gaacggttaa tttaagtact gatcttgcac actccacaac tcgttcgtgc tgaagccact
     2281 cgaagcatgg acgggttttc atatgggctg tcttcgcgga agcgactgta atttttggcc
     2341 ccgtgcacgg tcatgtgcgg tccggtttat tacaggcgca tcgtaatttg ttcagtccac
     2401 ccaatgcaag acgttacgaa tcaccgacca gcggggttgc ttgttcgccg cgaatcgacc
     2461 caggacccaa tagtcataaa gtttttttaa ggtgccgtca ctctttttga gatctatcaa
     2521 gacgttgacg taatcgacca gttcctgatc agcacgtgcc atcggatagc tcagcggtat
     2581 cgagatcgga ggtggcggca ccacaacggt gtattctgga tatagcagac tccaagccga
     2641 tccctcttcg gcggtataga taaacccatc gagcgtcttt cccttagatt cgaagaactc
     2701 gctcggggaa ttcagggtca ccagctgtgc tttgggcagg agctctcgga caaaggagat
     2761 aaaatagggc acatccaaaa ctgctaactt cggagctttt agtttttgca gcgcttcacg
     2821 gctgccaaat tcgcctcgtc gatagtcttg gacaacgaaa gctaaggtca tttccaagta
     2881 cggcgccgag agacggacaa cctccgcgcg ttctggagtc acgacgattc cggacattat
     2941 gatgtcacag taacctccat tgagctgttc aaacatcttg gagtgatcga cgggtacgaa
     3001 ctcgaggctc acgccaagag agcgagcaag aatatgtgcc atttcagcgt ccagccctac
     3061 cagctccccc tgtgcattga aaaaagagaa aggacgaacg tccccgatat atccgacccg
     3121 gatggctccc cggctgcgga ttcttttcat cgtcggctgc gacgattcca ctggcccagg
     3181 aggcggcgcc gatttgtaaa tcttggccgg gacggcaggg aataaaggct gcataagggc
     3241 catgattttg gccttgtcgt aggcgttctc aaccgcgaat gaaaagtaag cccgagtgcc
     3301 gacgacggca gccgcgacga cgccaaccgt aagcgctgtt actggaacga agcgcttcca
     3361 ctgaaagcgc aacgcccccg ccatcgcaaa gcccccgagc acccccaggg taaaaagatg
     3421 catcgccgac gccaaagttc ccagttgcgc gacgaaaatt cccgaggcga ggtaaagctg
     3481 catcatatca gcgggtaggc gaagcaaatc aagcagaaac ggcatggcgg caacaggttt
     3541 gccgaacagg ctaagcaggc cggacagggt taaattgaaa tatcctgcaa gactgatggc
     3601 cgacgtggtg aaccaagcgg cgaagaggat gaaaatgagc tggagcagcc tgccgacgtt
     3661 tggaaaattg aaggaaaccg gaacgatgac gtcaacgcta gactccgatt cggcactggt
     3721 acccatccct tgccgtagca attcctttgc atggtcactc aacatcggta tcacgaccag
     3781 cagattgtct gttgcgaaag ccattaacag ggcctctttt gtcgcccgga cgatctcccg
     3841 gtaaccgaat ggtgtgagca gcatgatcag agcagggagc acgccaaacg tcatcaagag
     3901 ggcggcaaca gcgtatgtga cgatataaac ctgcaagccg ctcaattgat cgacattcat
     3961 ggtgccggcc gtgctcgcgg caatcgcgaa aatcccgatc ggggtgagcc ggacaatgaa
     4021 tttcatgact cgcttgagcg cttcttccag gatttcaaag gcggcgatga gtgggccctt
//
LOCUS       DASSWZ010000122         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_668839, whole genome shotgun sequence.
ACCESSION   DASSWZ010000122 DASSWZ010000000
VERSION     DASSWZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668839"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFN58_03080"
     CDS             complement(<1..588)
                     /locus_tag="VFN58_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HET8917007.1"
                     /translation="MAQAQNEFSVVGKRIPRIDAYERVTGQAQYTGDIQLPGMLYARV
                     LRSPHPHANIVGIDTSKAEKLPGVKAVIHHGNAKIPWSSGGHTHPRFIFNNPVRFVGD
                     AVAAVAATDRHIAEEALGLIAVKYEKIPHVLDPNEALKPDAPKISTDGNLSVGKGNFS
                     APIEESWGDLEQGFKEADRVFEDTYITKHTNNAQLE"
     gene            complement(600..1220)
                     /locus_tag="VFN58_03085"
     CDS             complement(600..1220)
                     /locus_tag="VFN58_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8917008.1"
                     /translation="MKNEQKGNGLSRRSFLKGLGGGAIGTAVISTGLVKSDQAEAYTS
                     EAVKGAPGKSAVNLKVNGKSYRVEVEHRYTLAEVLRDQLGLTGTKIGCDRGECGACTV
                     IIAGRNMYSCSQLAVWMEGKEILTIEGLAKGDKLDPVQEAFIEHDGPQCGFCTSGQIM
                     SGKALLMKNSKPTEAQARRALSGNLCRCGNYNREVAAVLAAATKAG"
     gene            1355..2266
                     /locus_tag="VFN58_03090"
     CDS             1355..2266
                     /locus_tag="VFN58_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HET8917009.1"
                     /translation="MAERIPFQSEGEQLESFLDELQKEQQIKEIAGWETGFPNLSRAL
                     DGIRPGLYLLIGPPACGKTSFAKQLLDQVARQNAVPGVFFSFAECKKELRIKTLARLS
                     GIENREIRRGSVYLLHWYGMPKAQHTELERLPPSWDKLKKSAEEAKSWLDLIYLIECD
                     RDIDLRRIEDQIREVESIKGMDRVMVVIDDCQRLGNIEQKLTDRVVLLVDELQRAAVS
                     LQIPILAVCPDLREDRSSVPQIWSDRAPSADVILVMEIDLERTKKLIEPNQALTLHII
                     KNRGGERGKLAFEFVPAFSKFTEVAPA"
     gene            complement(2294..3031)
                     /locus_tag="VFN58_03095"
     CDS             complement(2294..3031)
                     /locus_tag="VFN58_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET8917010.1"
                     /translation="MPNRFLVRPGPSSSLVLKENEVHIWSVQTHGESISSGDFKELLS
                     SVEQDRASKFKFETDRRRYITAHAALRSILAIYVNGPARELQFVAGPYGKPKLAPNQA
                     KNKFEFNLSHSHEVALIAVTQDREIGVDVEWVRKDFAFDEVAQRFFTTREVAALHALP
                     LHLQREAFYKCWTSKEAFLKAKGTGLSGQLDEVEIVFIDHVVRVKGTVPNLSLIELPV
                     DGGYVAAMVVDGAEPRLTYHAWESRSK"
     gene            complement(3238..3558)
                     /locus_tag="VFN58_03100"
     CDS             complement(3238..3558)
                     /locus_tag="VFN58_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917011.1"
                     /translation="MADALPNDDEIIDSIKNAAGIILGVSENRSWIELFFEGDLMHTK
                     TVNLPAGSVFDIYVEEIPHKATVYEHPRTMIFFNGPCDVRIAREGNRIIVTGSARSQN
                     PSVT"
BASE COUNT          803 a          999 c         1020 g          855 t
ORIGIN      
        1 ttcgagctgc gcgttgttcg tgtgcttggt gatgtaggtg tcttcaaaca cccggtcggc
       61 ctccttgaag ccctgctcca gatcccccca cgactcctcg atgggcgcgc tgaagttgcc
      121 cttgccgacg gaaagattgc cgtcggtgga aatcttcggc gcatccggtt tgagggcctc
      181 gttggggtcc aacacatggg ggattttttc gtacttgacg gcgatgagcc caagcgcttc
      241 ttcagcgatg tgccgatccg tcgccgccac cgccgctacc gcatcgccga caaaacgcac
      301 tgggttgttg aagatgaacc gcgggtgcgt atggccgccg ctggaccagg gtatcttggc
      361 gttgccatga tgaatcaccg ccttgacccc cggcaatttc tcggctttcg aggtatcgat
      421 gccgacaatg ttcgcgtgtg ggtgcgggct acgcagcacg cgagcataga gcatgccggg
      481 aagctggatg tcgccggtat actgcgcttg cccggttacc cgctcatagg cgtcgatgcg
      541 cggaattctt ttgccgacaa cggaaaactc attttgtgct tgtgccataa cgacctcctt
      601 tagcccgcct tggtggcggc ggcgagaacc gctgcaacct cacggttata attgccgcac
      661 cggcaaagat tgccggacag cgcgcggcgc gcctgcgcct cggtgggttt ggaatttttc
      721 atcagcaacg ccttgcccga catgatctga ccggaagtgc agaagccgca ttgcggcccg
      781 tcgtgttcga tgaaggcttc ctgcaccgga tcgagcttat cgcccttggc caagccttcg
      841 atggtgagaa tctctttgcc ttccatccag acggccagct ggctgcagga atacatgttg
      901 cgaccggcga taataaccgt gcaagcgccg cattcgccac ggtcgcagcc gatcttggtg
      961 ccggtgagac ccaactgatc gcgcaacacc tcggccaaag tgtagcggtg ttctacttcg
     1021 acgcggtagc tcttgccgtt aaccttgaga ttgaccgccg atttgccggg cgcccctttt
     1081 acggcttccg atgtataggc ctccgcctga tcggatttca ccagtcccgt ggagatcaca
     1141 gcggtaccga tggcgccgcc gccgagaccc ttcagaaaag agcgccggga tagcccgttt
     1201 cctttctgtt catttttcat ggtcgctaac ctcctgtttc gaaccttgtc ttacgcgtcg
     1261 agacggctaa gggttagtat ggaagcccgg ctcatgtcaa tgaaaaaatg cggcttgctg
     1321 gaggcgcgga aaatcgataa gctcgcgcaa ttccatggct gaaaggattc catttcaatc
     1381 tgaaggagaa caactggagt cgttccttga cgagcttcaa aaagagcaac agatcaaaga
     1441 gattgccggc tgggaaacgg gttttcccaa tcttagccgc gctctggacg gtattcgccc
     1501 cggcctctat ctgctcatcg gtccgccggc gtgcggcaaa acctcgtttg ccaaacagct
     1561 tctcgatcaa gtagcaagac aaaacgctgt gccaggagtt tttttcagct tcgccgagtg
     1621 taagaaggaa ctccgaatca agacgctggc ccgtttaagt ggaatcgaga acagagagat
     1681 acgccgcggc agcgtctatc tcctccattg gtacggcatg ccgaaagccc aacacactga
     1741 gcttgagcgt ctgccaccca gttgggacaa gctcaagaaa tcagcggaag aagcgaagag
     1801 ctggttggat ttgatctatc tcattgagtg cgaccgagac attgatctga gacgaatcga
     1861 agatcagatt cgcgaggtcg aaagcatcaa aggcatggac cgtgtgatgg ttgtgatcga
     1921 cgattgccag cgcctgggaa acattgagca gaaactcacc gaccgcgtcg tgctactcgt
     1981 ggacgagttg caaagagccg cggtgagctt gcagattccc attctggctg tttgccccga
     2041 tttacgagag gatcgcagca gtgtgcctca gatctggagc gacagagcac caagcgcaga
     2101 cgtcatcttg gtcatggaga tcgatttgga acggaccaaa aaactcatcg aacccaatca
     2161 agcgctcact ctccacatca taaagaaccg tggcggggaa agaggcaaac tggcctttga
     2221 attcgtcccg gctttttcaa agtttaccga ggtcgctccg gcctgaaccc agtcacgcgt
     2281 cacgatagtc gagttatttg ctccgcgatt cccacgcatg gtatgtgagc cgaggttcag
     2341 cgccgtccac gaccatcgcc gccacgtagc ctccatcgac aggcagctcg atgagagaaa
     2401 gatttggcac tgttcctttg acgcgcacaa catgatcgat aaacacaatc tcgacttcgt
     2461 ctaattgccc tgagagaccc gtgcctttgg ctttcaggaa tgcttctttg cttgtccaac
     2521 acttatagaa cgcctcccgt tgaagatgga gtgggagagc atggagcgct gccacttctc
     2581 tggtagtgaa gaatcgttgg gccacctcgt cgaaggcgaa atcttttcta acccactcga
     2641 catcaacgcc gatctctcga tcttgagtca cagcgatcag cgccacctca tgggaatggg
     2701 acagattgaa ctcaaacttg ttcttggcct ggttgggcgc cagtttcggc ttaccgtaag
     2761 gacccgcgac aaactgcaac tcccgagcag ggccgtttac ataaatggca aggatcgacc
     2821 tcagtgcagc gtgggctgta atataccgtc gccggtcggt ttcaaatttg aatttcgagg
     2881 ctcggtcttg ctccaccgaa gacaagagct ctttgaagtc gcccgaggag atgctttcac
     2941 cgtgggtttg gactgaccaa atatgaactt cattttcttt tagaacgagg cttgaggagg
     3001 ggcccggtcg aaccaaaaac cgattgggca tgctaagtgc ttttacctga ttcgctcgcc
     3061 ctaatccacg cttaacgcaa ccagctatct aactcgattg tgcctggttt cgtatgcttg
     3121 accatctaac tgaatgccgg ttgctcagcc ggcagacaaa ggccaagatc tttgcaacgg
     3181 ctttatagat ctttggcagg atttcgtcgt tgtgaatttt ggtgtgattc tggctactta
     3241 ggtcacgctg ggattctggc tccgagcact cccggtgacg atgatgcggt tcccttcacg
     3301 ggcaatgcgg acgtcacaag gcccattgaa gaagatcatc gtgcgcgggt gctcgtagac
     3361 agtggctttg tgaggaattt cttctacgta gatatcgaag acggagccgg cgggaagatt
     3421 gacggtcttg gtgtgcatca ggtcgccttc gaagaaaagc tcgatccaac tgcggttttc
     3481 ggaaaccccg aggatgatac cggcggcgtt tttgatcgag tcaataattt cgtcgtcgtt
     3541 gggaagagcg tcggccatgc aatcgacctc tagttcgaga gcagggtatc ggcctcccgg
     3601 atgagttttc ccgacgaagt acgcggcagc gagtcgcgga caacgatctt ttgcgggact
     3661 ttgaagttcg ccagatt
//
LOCUS       DASSWZ010000123         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_102810, whole genome shotgun sequence.
ACCESSION   DASSWZ010000123 DASSWZ010000000
VERSION     DASSWZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102810"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFN58_03105"
     CDS             complement(<1..824)
                     /locus_tag="VFN58_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8917012.1"
                     /translation="MVKRDTSYEAWLREEGIPIIEGYGVENVTDLPRKPWARTGGQGA
                     YIQLTGMEGFTGMYVGEIPAGGSLNIERHLYEKIIYILHGFGATEVWSGNSEKKKIQF
                     EWQQGSLFAVPLNCSHRMINGSREPAIFLAVTSAPLMIDLLHDVPFVFGCDYTFDERF
                     DGQADYFVPTSNRQFQGGFFNWESNFIADARGALVDPSEAKGYGVRITSFDMGGSTLV
                     GHLAEWPVGRYHKAHFHTGGAILLILRSEGYTLMWPQEAGIRPYKSGNGDQVVKVN"
     gene            complement(842..2425)
                     /locus_tag="VFN58_03110"
     CDS             complement(842..2425)
                     /locus_tag="VFN58_03110"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8917013.1"
                     /translation="MNLGAENYFTDFRAFLDDLERRGKLYRWRQRVNKDTELMPLMRL
                     QYRGLPDDKRQVLLFEKVCDGNGRQFSVRVATGMYGSSRQILGLGMGCEDPGAIYEKW
                     HRALAKPLPPRLVDFSPLHEEVYSGSRLSQFGLTDLPAPVEEPGFSCGIRFTAPFITK
                     DPETGMRNVGMYSGHFRGKHKLIAGIARTHHAMLYHHPAAVKRREPLPVAIVLGALPD
                     INYVAAANLPYGLDELEVAGAVRGRPVDLVRCKTIPLEVPAEAEIVIEGEISLDAMER
                     GEPFSDYPGYLMAERGLRPVIDVKAITHRRDPIFTAILVGLPPSESNGISRTCREMML
                     YNFLKYSCNLPEVLEVCCPEMGGGWNWWVIRMAKSHPSKPKQALQAASGMDTTNKVII
                     VVDEDIDPKDPDMVMWAISFAMQPHRDVQVITGRSPLLDPSAYALMNNPEERFYPPPV
                     GCGGLLIDATRKGKYPPVGLPKKKYMERALEMWREVKMPELQLKTPWYGYPLDLWTTD
                     DDALAEAVASGNYFPSAKK"
BASE COUNT          611 a          787 c          673 g          625 t
ORIGIN      
        1 caattcactt taaccacctg atcgccattg ccggatttat atggacgtat acctgcttcc
       61 tgcggccaca tgagggtgta accttcggag cgaagtatca aaagaatcgc gcccccggta
      121 tgaaagtggg ctttgtgata gcgtcccacc ggccactcgg cgaggtgacc caccaaggta
      181 ctgccgccca tatcgaaaga ggttatgcgc acgccgtaac ccttcgcctc ggaggggtcc
      241 acgagcgcac cgcgtgcatc ggcgataaag ttcgactccc agttaaaaaa accgccttga
      301 aattgccgat tcgatgtcgg cacgaaataa tcggcctggc cgtcaaagcg ctcatcgaat
      361 gtataatcgc aaccaaacac gaacgggacg tcgtgcagca gatcgatcat caaaggcgcg
      421 ctcgtcacgg ccaagaaaat agccggttcg cggctgccat tgatcattcg gtgcgaacag
      481 ttaaggggaa ccgcaaacaa gcttccctgc tgccactcga actggatctt ctttttttcg
      541 ctattccccg accagacttc ggtcgctcca aagccatgaa gaatataaat gatcttttcg
      601 tagagatgcc gttcaatatt cagggacccg ccggcgggaa tttcaccgac gtacatgccg
      661 gtgaatcctt ccatgcccgt gagctgaata taagcaccct gcccgccggt tcgcgcccag
      721 ggctttcgcg gcaagtccgt aacgttttcc accccatacc cctcgatgat tggaatgcct
      781 tcttcgcgca gccaggcttc gtaactcgta tctcttttca ccacaaagac accgtccttt
      841 cttatttctt cgccgaagga aaataattgc cgctggccac agcctcggcc aacgcgtcgt
      901 catccgttgt ccaaagatcc aatggatagc cataccaagg cgttttgagc tgcagctccg
      961 gcattttcac ttcacgccac atttccaggg ctcgctccat atactttttc ttaggtaggc
     1021 ccacgggtgg gtacttgcct ttccgtgtgg catcgatcaa caacccgcca catcccacgg
     1081 gcggcggata aaagcgctct tccggattat tcatcagcgc atacgcggag gggtccaaca
     1141 gaggggaacg gccggtaatc acttgcacat cccggtgggg ttgcatcgca aagctgatgg
     1201 cccacatcac catatccgga tccttgggat ctatgtcttc gtcgacgaca atgatgacct
     1261 tgttagtcgt gtccataccg gaggccgcct ggagggcctg cttgggttta ctcggatgac
     1321 ttttggccat ccggatcacc caccagttcc atccgccacc catttccggg cagcagacct
     1381 cgaggacttc cggcagattg cagctgtatt tgagaaagtt gtagagcatc atctcgcgac
     1441 aagtacgcga gataccgttg gactcgctgg gtggcagccc gactagaatc gcagtaaaaa
     1501 tcgggtcgcg ccggtgggta atggctttta catcgatgac cgggcgcaac ccccgttctg
     1561 ccatgagata accgggataa tcgctgaagg gctcgccccg ctccatagcg tcaagagaaa
     1621 tttccccttc tatgacgatt tccgcttctg ccggaacttc cagtggaatc gttttgcagc
     1681 gcaccagatc gacgggccgt ccccggaccg cgccggcgac ttccaattca tcaagtccat
     1741 agggcagatt cgcggcagct acatagttga tgtctggcaa cgctcccaag acaatggcca
     1801 cagggagcgg ctcacggcgc tttaccgcag ccggatgatg atagagcatt gcgtgatgag
     1861 tgcgcgcgat accggcgatg agtttatgct taccgcggaa gtgaccgctg tacatgccga
     1921 cgtttctcat tccggtttcg ggatctttgg taataaatgg ggccgtgaag cgaatcccgc
     1981 agctgaaccc cggctcttcc accggtgccg gcaaatccgt tagaccgaac tggctcaatc
     2041 tggagccact atagacttct tcatgcaagg gactgaaatc gacgaggcgt ggcggcaggg
     2101 gtttggcaag cgcccggtgc cacttttcat agatggctcc gggatcttcg caacccatac
     2161 ccaggcccag gatctgcctc gacgatccat acatgccggt ggcaaccctg acagagaatt
     2221 gtcttccgtt cccgtcgcaa accttttcga atagcagaac ctgtctcttg tcgtccggca
     2281 ggcctcggta ttgaagacgc atcagcggca tcaattcagt atctttgttg acgcgctgcc
     2341 gccatcggta caactttcct cgtctctcca gatcatcaag aaaggcccgg aaatcggtaa
     2401 aatagttttc agctcctaga ttcatttttt ctccgcggta agttaaatct gcgttgatcc
     2461 actacggcag aagtttccga gccaccttca atgcgtactc gcgaactttg tccaactttt
     2521 cttccgattg attttctatc tggagatagt ctttcactga catgtggtcc ggagcttgtt
     2581 acgctcttac tctgagaatg atttatcggt ctgggcaaac gaccaagggg caaacaacag
     2641 agcccaccca aataacgcgg tccccaatcc gaacaatctc atgaagtttc caaagc
//
LOCUS       DASSWZ010000124         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_202098, whole genome shotgun sequence.
ACCESSION   DASSWZ010000124 DASSWZ010000000
VERSION     DASSWZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202098"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VFN58_03115"
     CDS             <1..1111
                     /locus_tag="VFN58_03115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917014.1"
                     /translation="SALRLLGIFFMLPIFSAYAVRYPGASIGLAGIAFGIYALTQCIF
                     QIPLGWASDRWGRKPVLVIGLALFGLGSIGCGLAQNIFQLIIARMIQGTGAVGSVALA
                     ALADLTRPTVRTQAYTVTGIAIGSAFMIGILGGPLLAASIGLSGLFYVLAALGFLAMI
                     LAATSFPQERQSEPQEPPMAFKPILLHGELRPLFIATFVLSFALNLFFFTYPLSWTEL
                     GLEKAELWKVYLIIFLPSVLLVFPYVRRAEKRGRFRLPIFIGWFAATLGYLLYLVGAQ
                     HDFLLYLSGAAFFFGYSLYQPILPAFLTQRIPPGGRGTATGVYTFFGFIGSSLGGMLG
                     GGLLHISPSLPEFVGVMLLIIWYFLGLPSRPDSIS"
     gene            1406..1792
                     /locus_tag="VFN58_03120"
     CDS             1406..1792
                     /locus_tag="VFN58_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917015.1"
                     /translation="MRRWLRLTACLICITVGTSGCGGSHEETVGGVKIPIPGGMTRSQ
                     ESGIEVSLPGFGGAQAAYQGKMDPDKVIEFYRKEMPARGWQPSLGLLSKGGMLTYIKD
                     NTTVIITVGKTDSGTGMTIIAGGTQR"
     gene            1851..2327
                     /gene="bfr"
                     /locus_tag="VFN58_03125"
     CDS             1851..2327
                     /gene="bfr"
                     /locus_tag="VFN58_03125"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET8917016.1"
                     /translation="MKGSEQVIAVLNQVLRKELTGINQYFIHAKMCQNWGYERLYKAL
                     WDESIGEMKHADQIIERILFLEGVPNISAYDKILVGTNVQQQLENDLSLEGAALTVLH
                     PGIKTCVEAGDDSSRELLEHIVVDEEKHVDWIEAQLHQINEVGYQNYLTQQIIKKD"
     gene            complement(2446..3312)
                     /locus_tag="VFN58_03130"
     CDS             complement(2446..3312)
                     /locus_tag="VFN58_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET8917017.1"
                     /translation="MLAIAFGILASLCFAAASLLAQRGLYIVPTPWGAWVTLVANTIF
                     LLAFHFALYRNASIFVLDNLTFVAVGLFVPGMTRVLTFRGIGTMGSAITSTIVNTTPM
                     FSTALAMLVLGERPGPLVLAAVVLIVGGLAIISWEGAERSWKRTELIFPFLAALLFAM
                     KDVTVRWGLGGSSSPVLAAAIAAFTSTIEIFLINLYIYKEKFALPPKQVIHWFVGSGL
                     FTGGSFLFMYLAFNMERVSIVAPLINSYAVFVLVLAPLMAKQIEKVTLRKIVGAVLVV
                     AGIFLISMGRSE"
     gene            3389..>3872
                     /locus_tag="VFN58_03135"
     CDS             3389..>3872
                     /locus_tag="VFN58_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET8917018.1"
                     /translation="MALKNLSVIRRKLLRWYDQHRRDLPWRRTRDPYAIWIAETMLQQ
                     TQVATVAPYYERFLRELPTVHALDRAPLSRILALWSGLGYYRRAENLKKSAREIVRKY
                     GGHLPDDYDALIALPGVGDYTAGALLSIAFQKSYPAIDGNAHRVLHRLFTPKNDRKLR
                     Q"
BASE COUNT          903 a         1057 c         1010 g          902 t
ORIGIN      
        1 ttcagcactg cgactgctgg gcattttttt tatgctgccg atctttagcg cgtacgccgt
       61 tcgctacccc ggagcgagca tcggcctcgc ggggatagcc ttcggaattt atgctctcac
      121 acaatgtatc tttcagatcc cgttaggctg ggccagcgac cgctggggac gcaaaccggt
      181 attagttatc ggtctggcat tgttcggctt gggcagcatc ggatgcggac ttgcccagaa
      241 catttttcag ctgatcatcg cccgcatgat ccaaggaacc ggcgcggtgg gctcggtcgc
      301 cttggccgcg ttggccgacc ttactcgacc cacagttcgg acacaggcgt acacggtcac
      361 ggggatcgcc atcggatcgg cgttcatgat cggcatactc gggggtccgc tattggctgc
      421 aagcatcggc ctgagtggcc ttttttatgt tttggccgcg cttggcttct tggcgatgat
      481 tctcgccgcc acttcatttc cgcaggaacg tcaatccgaa ccgcaagagc caccgatggc
      541 ttttaaaccc attctcttac acggcgagtt gcggccccta ttcatcgcca catttgttct
      601 ttccttcgcg ctgaatctct tcttcttcac ttatccgctc agctggacgg aacttggcct
      661 cgaaaaggcc gagctttgga aagtttatct catcattttt ctacccagcg tcctgctagt
      721 ttttccttac gtgcgacgcg ccgagaaacg cggccgtttc cgtctgccga tttttatcgg
      781 ctggttcgca gcgactctgg gttatctact ttatctggta ggggctcagc acgacttctt
      841 gctgtatttg agcggcgccg cttttttctt cggctacagt ctctatcaac ccatcttgcc
      901 ggcctttctc acgcagcgaa tacccccggg aggtcgcggc acggccaccg gtgtctatac
      961 gttttttggg ttcatcggat cgtcgctcgg aggcatgtta ggtggcggac tgctccatat
     1021 ctctccgtct cttccggagt tcgtcggcgt tatgctcttg atcatctggt atttcctagg
     1081 cttgcccagt cgacccgatt ccatttccta aggagcctgc tgaaaaaggc ccatctactt
     1141 cgttgcgctc actcgcctcg ctacagttgg cgtatggcat atggcttatg gctttatagc
     1201 gtatggtttg ttgaatccct ctatctgcca taagctatca gctatatgcg atttctcgtg
     1261 cgcgcctcgt agccgagacc tttttgacca ggctccaagt ctttcggtaa cctttaatct
     1321 cacgctccgt ggtcactgaa tttgaattga acagaccaga acattgatgt acgaaccaat
     1381 aaaaagacat ataatgagaa ccgagatgag aaggtggctg cgactcaccg cctgtttaat
     1441 ttgcatcacc gtgggtacgt ccggctgcgg cggttctcat gaagagactg tcggaggcgt
     1501 caagattcca atacccggtg gaatgaccag atcccaggaa agcggcatcg aggtgtcgtt
     1561 accgggcttc ggtggtgctc aagccgcgta ccagggaaaa atggatccgg acaaggtcat
     1621 cgagttttac cgaaaggaaa tgcctgcgcg tggctggcaa cccagtttag gattgctgtc
     1681 taagggaggt atgctaactt acataaagga caacacgaca gtgattatca ccgtaggaaa
     1741 aaccgacagc ggcaccggca tgacgatcat cgccggtggg acccagcgct gagcgttgga
     1801 ccgctcgcgc ctactactca ataaatttga cataaagaaa gggacttacc atgaagggga
     1861 gcgaacaagt gatcgcggtc ttaaaccaag ttttgcgcaa agaattgacc ggcatcaacc
     1921 agtactttat ccatgccaag atgtgccaaa actggggcta cgagagactt tacaaagccc
     1981 tctgggacga atcgataggt gaaatgaaac atgcggatca aattattgaa cgcattctct
     2041 tcctcgaggg cgtccccaat atctcggcct atgacaagat tttggtcggt actaacgtgc
     2101 agcaacagct ggaaaacgat ctctccctgg agggagcggc gctaacggtt cttcatcccg
     2161 ggataaagac ttgcgttgaa gccggcgatg actcatctag ggagctattg gagcacattg
     2221 ttgtggatga ggaaaaacat gtagattgga ttgaggctca gcttcaccaa atcaatgaag
     2281 tcggatatca aaactacctg acacagcaaa tcatcaagaa agactagcga agtgctgaaa
     2341 agttctcgat tcaccctcga gagcctgaca tgatggtgat tttccgttca tgctgattgg
     2401 gtggcgacca gggaagcgcg gggcatcgat cgctgacggt cgttgttact cacttctccc
     2461 cattgaaatc aagaaaattc ccgcaacgac aagcaccgct ccgacgattt tgcgtaaagt
     2521 aactttttca atctgctttg ccatcagcgg tgccagaacc aggacgaata ccgcgtagct
     2581 attgatcagc ggagcgacga tggagacgcg ttccatatta aacgcgagat acatgaagag
     2641 aaacgacccg ccggtaaata gcccggaacc gacaaaccag tggatgacct gcttcggcgg
     2701 gagagcgaat ttttctttgt agatgtaaag gttgatcaag aagatttcga tggttgacgt
     2761 gaaagcggcg atggcggcgg ccaagaccgg tgaactgctt cctcccaacc cccaccgcac
     2821 cgtgacatct ttcatggcga ataatagggc ggcaagaaac ggaaagatta gctccgttct
     2881 tttccacgag cgctcagctc cctcccagga gatgatcgcc agcccaccga cgatgagaac
     2941 cacagcggca agcaccagag gacctgggcg ctcccccagt accagcatcg ccagcgctgt
     3001 ggaaaacatc ggcgtcgtgt tgactatggt cgacgttatc gcactgccca tcgttccaat
     3061 cccgcgaaac gtcaagactc gagtcatgcc gggcacgaaa agcccgaccg ccacaaaagt
     3121 aagattgtcc agcacaaaga tcgatgcatt gcggtaaagc gcaaaatgaa acgccaatag
     3181 aaaaatggtg ttcgcaacca gcgtaaccca ggcgccccac ggagtaggca caatgtagag
     3241 accgcgctgc gcgagcagac tggcggccgc gaaacaaagc gaagctaaga tgccgaacgc
     3301 gatggcaagc atagaggcct gttgttttct ttcccagatt ttttcgttta atctacccca
     3361 atactaaaat tccttgccga tttcatcgat ggcgctcaag aatctttcgg tcatccgccg
     3421 caagctgctc cgctggtacg accagcaccg ccgagatctt ccgtggcgca gaacccgcga
     3481 tccttacgct atctggattg ccgagacgat gctccagcag acccaggtcg cgacagtcgc
     3541 gccgtattat gaacggtttc tgcgtgagct gccgaccgtc cacgccctag accgagcgcc
     3601 ccttagcaga atcttggctc tatggtccgg gctcggctac tatcggcgag cggagaatct
     3661 gaaaaaaagc gcccgtgaga tcgtgcgcaa atatggcggg caccttcccg acgattatga
     3721 cgcgcttatc gcgctgcctg gtgtcggcga ctacacagca ggggctttat tgagcatcgc
     3781 ctttcagaaa tcatatccgg ctatagacgg caacgcgcat cgagtcttgc accgcctctt
     3841 cactccgaag aacgatcgga agctgcggca ag
//
LOCUS       DASSWZ010000125         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1206813, whole genome shotgun sequence.
ACCESSION   DASSWZ010000125 DASSWZ010000000
VERSION     DASSWZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206813"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VFN58_03140"
     CDS             complement(<1..892)
                     /locus_tag="VFN58_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917019.1"
                     /translation="MMNSTSMNDLKRLRIDHIGSLVRPAKLKEAFARYDRGRLSREQL
                     GLTQEEAIREVIVGQEAHGFPVVTDGEFRRHSFQESFSEAVSGFDVPKNIGVYYEQRQ
                     LNQNPLERAEQNFDETGPAIVTRRPAVERLKLVRNIPLEEFRFAQSVAKKPVKVTLIG
                     PDRVAQRFKWEASMDVYKGLDDFVEHIVAIQQQMISELVEAGCKYIQIDAPGYTAYVD
                     RVSLDRMRSRGENPERNLQRSIDADNAVIDGFPNVTFGIHVCRGNARTIDPKTGKLVP
                     QWHREGHYDAIAERLFTSLKH"
     gene            complement(931..2100)
                     /gene="hemW"
                     /locus_tag="VFN58_03145"
     CDS             complement(931..2100)
                     /gene="hemW"
                     /locus_tag="VFN58_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET8917020.1"
                     /translation="MPAGQEPFSLYVHIPYCISKCPYCDFNSHVVPEIPEANYTEALI
                     QELNQYARVNHWRDRSVQTIFFGGGTPSTFQPSSIARILERANVLFPIDSACEITLET
                     NPGTVESANFAGYRSYGVNRISVGVQSFQPHLLNFLGRVHSADEAVKALQVIGDAGFE
                     NFSLDLIYANPGQTLRDLENDLETALGFQSPHLSAYNLTIEEGTPFHHEYRSGRIRLL
                     SEDQEIAMAELIEQRLSDAGLQRYEISNYARPGFHSRHNLNYWQSGDYLGIGAGAHSH
                     KRVANHGIYGQRWWNEKHPARYMSKISESGQAVIDAEESDLSRAAGEYMFSGLRLMEG
                     ISLKAFSVRFGKNILALYPQISGWVSEGFMETEGEWLRLTHRGILVANSIFVHFV"
     gene            complement(2156..2926)
                     /locus_tag="VFN58_03150"
     CDS             complement(2156..2926)
                     /locus_tag="VFN58_03150"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF014199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HET8917021.1"
                     /translation="MKFRVLGCSGGQLPGYNLSSFLIDDSLLIDAGSTTAALTLAAQQ
                     KIRDILVTHIHLDHVMALGTLADNLYGKCKIPINVWSVRRVIDGLAKSFFNNKVWPDF
                     TRITGPTQPVPVLRLQSLREGKVTTIGGHSITAIGVGHVVPTVAFLVESKNKTLLHVG
                     DTGPTEKIWSFARKHPDLGSLVIETSFPNRLQDVADVSRHLTPRTLLQELDKLAMKSP
                     QILITHLKPEFRQEVIRELRKLKGHRLRVLRDGDVLQL"
BASE COUNT          746 a          860 c          907 g          797 t
ORIGIN      
        1 gatgcttcag gctcgtgaat agccgctccg cgatggcgtc gtagtgaccc tcgcggtgcc
       61 actgcggaac gagcttgccg gtcttgggat cgatggtgcg tgcgttgccg cggcagacgt
      121 gaatgccgaa agtcacattg gggaagccgt cgatcactgc gttgtcagca tcgatggatc
      181 gctgcaagtt tctctccggg ttctccccgc gcgagcgcat tctatccaga gatactcgat
      241 caacgtaagc cgtatagccc ggcgcgtcga tctgaatgta cttgcagccg gcttctacca
      301 gctcagagat catttgctgt tggatcgcga cgatgtgctc gacaaaatcg tcgaggccct
      361 tgtacacgtc catggaagct tcccacttga agcgctgcgc cacgcgatcg gggccgatga
      421 gggtcacttt cactggtttc tttgcgacac tttgagcgaa gcgaaactct tctagaggaa
      481 tatttctaac gagcttcagg cgctcgacgg cgggccgtcg ggtaacaatc gccggacctg
      541 tctcatcaaa attctgctcc gcccgttcca gcgggttttg gttgagttgc cgctgctcat
      601 agtacacacc aatattcttt ggtacgtcga aacccgacac cgcctcggaa aaactttcct
      661 gaaaactatg gcgccgaaac tcgccgtccg tcaccaccgg aaacccatgc gcttcctgtc
      721 caacgatcac ttcacgaatc gcctcctcct gggtgaggcc taattgttcg cggctgagcc
      781 gcccgcggtc gtagcgagcg aacgcttctt tcagttttgc aggtcgaacg aggctcccga
      841 tatgatcgat ccgaagtcgt ttcaaatcat tcattgacgt tgaattcatc atgctcgcct
      901 ccgttggatt tgatctatag ccggcaactg ttaaacgaag tgcacgaaaa tggagttggc
      961 caccaaaatg ccgcggtggg tgagtcgaag ccattcgcct tcggtttcca tgaatccttc
     1021 gctgacccaa ccagagatct gcggatagag cgccaatatg tttttgccga agcgcacgga
     1081 aaaggctttc agcgaaattc cctccataag ccttaggccg gaaaacatat actctcctgc
     1141 ggctctgctt agatcgcttt cttctgcatc aatgacagcc tgtccacttt cgctgatctt
     1201 gctcatatag cgagccggat gtttctcatt ccaccaacgt tgtccgtaga ttccatgatt
     1261 tgcaacacgc ttgtgactat gtgcaccggc accgattccg agataatcgc cgctctgcca
     1321 atagttgagg ttgtgccggg aatgaaaacc aggccgcgcg tagttggaaa tctcgtagcg
     1381 ctgcaaaccc gcatcgctca gtcgttgttc gatcagctct gccatggcga tctcttggtc
     1441 ttcgctcaag aggcgaatac ggccgctgcg atactcgtgg tggaagggag tcccttcttc
     1501 gatggtgagg ttgtaggccg agagatgggg cgactggaag ccgagggcgg tctcaaggtc
     1561 gttttcgaga tctcgcagag tctggcccgg gttggcgtaa atcaggtcga ggctgaaatt
     1621 ctcgaatccc gcgtcgccga tgacctgcaa tgctttcact gcttcatcgg cggagtgaac
     1681 cctgccgaga aaattgagca ggtggggctg gaaagactgt acgccgacgc tgatacgatt
     1741 caccccgtag gagcggtagc cggcaaagtt tgcactttcc acggtgccgg gattggtttc
     1801 caaagtgatt tcgcaggcag agtcgattgg aaacaacacg tttgcgcgtt cgagaatccg
     1861 cgcaatgctt gacggctgaa aggtcgacgg cgtacccccg ccgaagaaga tcgtttggac
     1921 acttctatcg cgccaatgat tcacacgggc atactgattc agctcttgaa tcaatgcttc
     1981 tgtgtaattt gcttccggga tttccgggac gacgtgggaa ttaaaatcgc agtagggaca
     2041 tttgctgatg caatagggga tatgaacgta aagggaaaag ggctcctgtc cagccggcat
     2101 agccctatca tattgaccaa aggagggcgc tacaacacct caagaattat tgtggctaaa
     2161 gctgcagcac gtcgccatcc cgtaacacac gaaggcggtg gcccttgagt ttgcgcaatt
     2221 ctctgatgac ctcttggcgg aactcaggtt taaggtgagt aatcagaatc tgcggcgatt
     2281 tcatcgcgag cttgtcgagt tcctgaagta aagtccgagg tgttaaatga cggctcacgt
     2341 cggcgacgtc ctggagccgg ttcggaaaag aagtttcgat gacgagcgag ccgagatctg
     2401 ggtgttttcg ggcgaaggac cagatctttt ccgtaggtcc ggtgtcgccg acatgaagca
     2461 aggttttgtt cttcgactcg acaagaaaag caactgtggg caccacgtgt ccgaccccga
     2521 tcgcggtaat ggaatggccc ccgatcgtag ttactttccc ctcccgtaaa gactgaagtc
     2581 tcagcacggg aactggttgg gttgggccgg taatgcgcgt gaaatctggc cacaccttat
     2641 tgttgaaaaa ggacttggcc aatccgtcga tgacgcgccg cacgctccaa acgttgatgg
     2701 gaattttaca tttgccgtag agattatcag ccaaagtgcc aagcgccatc acgtgatcca
     2761 gatggatgtg agtcacaagt atatctctga ttttttgttg cgctgcgaga gtcaacgccg
     2821 cggtggtgga gccggcgtca ataagcagcg agtcatcgat gagaaacgag gagaggttat
     2881 agccggggag ttgacctcca gaacaaccta gaacgcgaaa tttcaccgct attctccttc
     2941 ttccctcgtt gacttcacgg ctcccacggc taccatctta catcaatgcg gctgtttcgt
     3001 atccattttt tttcataggt tcccagggat ggctttgagc ccaaagagat attagacact
     3061 gagtgaattg taaagggaag agaggtcaag aggcgaatct tttgtactgg cgcaaagggt
     3121 gaaaaagagg taagggatgc tgcaaagaag tccatctgat ttcgattcga gatagggaga
     3181 acctgcatga cagagacacg agtgctcatt ctttcagtcc taacgcttta cttgttcggt
     3241 gcgtgcatca tctatgctgg ggaagcccct cgcatgtccc gcgtcggtat tctgatcccg
     3301 gaatccggcc
//
LOCUS       DASSWZ010000126         5561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_711451, whole genome shotgun sequence.
ACCESSION   DASSWZ010000126 DASSWZ010000000
VERSION     DASSWZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711451"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VFN58_03155"
     CDS             complement(<1..1483)
                     /locus_tag="VFN58_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8917022.1"
                     /translation="MAEVFRNYIDGAWVGSKSGKTFENHNPANRHDLIGLFPASSPED
                     VNGAVQAAKKAFALWRLVPAPKRGEILYRVGELLRRHKEEIAHGMTREMGKILKETRG
                     DVQEGIDTAFYVAGEGRRLFGETTPSELPDKFAMSMRSPIGVCALITPWNFPMAIPTW
                     KLFPALLCGNTVVLKPAEDTPYTAVKLFEILAEAGVPPGVVNLVHGRGEDVGAALVRH
                     PDVKLVSFTGSAAVGREIASVCGQQLKRVSLELGGKNAQIVMEDADLNLALEGALWGA
                     FGTTGQRCTATSRLILHRDIKKELTDMLVARAEKIKIGDGLDESVEMGPLINHAAREK
                     VHQYVQIGKGEGARLLIGGSIYEEGKWIDGYFYRPTIFDQVTPSMRIAQEEIFGPVLS
                     VIEVRSFDEAIEVINGTTYGLSSSVYTRDVERAFRAMRDIEAGITYINGPTIGAEVHL
                     PFGGVKDTGNGHREAGTTVYDIFSEWKSVYIDYSGKLQKAQIDN"
     gene            1748..2773
                     /locus_tag="VFN58_03160"
     CDS             1748..2773
                     /locus_tag="VFN58_03160"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8917023.1"
                     /translation="MGKKLILLAVALMLSFETASAASGDDFYKGKTIRIVVGFSAGGG
                     FDTYARALARHLSKHIPGQPAIVVENMTGAGSLIAANHVYKVAKPDGLTIGHFIGGLF
                     LGQVLGQKGVEFDARKFEYLGAPITDHVVCAMTKASGITNVETWMASKTPVKMGGIAP
                     GTSTPDNATRILKAAVGLPIQLVTGYKGTADVRLAAESGEVAGGCWGWDSVSVTWRKA
                     LDSGDAVVVLQANRRTHPDLPQVPQAIKLAKTDEGRKMIDVGIHSDSDIVRTYTLPPG
                     TPKDRVQLLRKAFEDTLKDPEFLADAKKSKLNVDPVPADVIEKDISALFKLDPATVGK
                     LKDVLYN"
     gene            2848..4863
                     /locus_tag="VFN58_03165"
     CDS             2848..4863
                     /locus_tag="VFN58_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET8917024.1"
                     /translation="MLEMFSALFAGLIQVFNWSTFSLMIIGIAVGFVVGILPGLGGPT
                     AMALMLPFIFKMSAVEAFAFLLGMTAVTATTGDITSVLFGVPGEPTTASTIVDGHAMA
                     RNGEAGRALGAVLMSSLVGAIFAGVALAVAIPVVRPIVLSFGSPEFFMLSLLGITFVA
                     SLSGEDQLKGIVAGGIGLMLATIGLDPISGIQRYTFGQLFLWDGIGLVPITIGFYAIP
                     ETIELAVLGTSIAQEKVEKIGGVMEGVKDTFRHWWLVIRCSALGTFTAIIPGMGAATT
                     QWLAYAHAVQSSPDKERFGKGDVRGVLGPGAANNSTLGGSLITTIAFGVPASVVMAIL
                     LGAFIIQGIVPGPDMLVPPPKGKLDLTFSFVWVIIISNFITVAVCFLFLNTLAKVTQV
                     RGSLIIPLILLLIYLGAFAEKNAFQDMIVVLFFGGLGWIMEKLHWPRPPVLLGLVLGP
                     LAENRLFLASDNYGMAWTWRPGVLIIFAVTLVGILYPLIKKKREEKTSRTAATSVEAT
                     PERGLQFGWPVVFTACVIILLALALWQSRNFGVRAGLFPWVIGTPTLLLALIQLAKDL
                     RGHRNVSGPAFEAEGAVEVSPEVATRRTLTILGWTVGFFVAIWLLGFSYAVPLTMVLY
                     LKLAGREKWPITVIMTFCTWGFFYLLFQKMLNVPFPDGLIFDLFKGQ"
     gene            complement(4912..>5561)
                     /locus_tag="VFN58_03170"
     CDS             complement(4912..>5561)
                     /locus_tag="VFN58_03170"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8917025.1"
                     /translation="GAKVLKATPTRLTLKHGRIEGPKGQSISFEDLMHKAFSSKAGEI
                     VGRGSYQDIKSKKAALGSPTTFWEISWGGAEVEVDRDTGEIKLIKYVSLADVGQAIHP
                     VLCEGQDEGGVMNALGHSLLEEMVYRDGQLLNPNLVDYRVPTFSDLPIGFETILVENH
                     NGPGPFGAKGTGEGGLLPVAPAIGNALYKATGVRLYRLPLTPERVWHALRAKNQS"
BASE COUNT         1121 a         1587 c         1529 g         1324 t
ORIGIN      
        1 cgttgtcgat ctgcgctttt tgcaactttc cggagtagtc gatgtaaacc gatttccatt
       61 cgctaaagat gtcgtacacg gtggtgccgg cctcccggtg gccgttgccg gtatctttca
      121 cgccgccgaa aggaagatgg acttcagcac cgatggtggg gccgttgata taagtgattc
      181 cggcttcgat atcccgcatg gcgcggaaag cgcgctccac atcgcgcgtg taaacggagc
      241 tggaaagacc gtaggtcgtg ccgttgataa cttcgatagc ctcgtcgaag ctcctcacct
      301 cgatgacgga gagcacggga ccaaagattt cttcctgcgc aatgcgcatc gacggcgtca
      361 cctggtcaaa gatcgtcggc cggtagaaat aaccatcgat ccattttccc tcttcataga
      421 tcgagccgcc gatgagtagc cgggcgcctt cccccttgcc gatttgcacg tattggtgca
      481 ccttttcccg tgccgcatga ttaatcagcg gacccatttc gacgctctcg tccagtccgt
      541 cgccgatctt gattttctcg gcgcgcgcca cgagcatgtc ggtcagctct tttttgatat
      601 cgcgatggag aatcaaacgg ctggtcgcgg tgcagcgctg tcccgtggtg ccgaaggctc
      661 cccagagtgc tccctccaaa gcgagattta aatcggcgtc ttccatgacg atttgagcgt
      721 tcttaccccc gagttccagg gagacccgtt tgagttgctg gccgcagacc gatgcgatct
      781 cgcgccccac cgcagcagag ccggtaaaag aaaccagctt cacgtcggga tgacgcacca
      841 atgccgcgcc gacgtcctcc ccacgaccgt gaaccaagtt aacgacacct ggcggcacgc
      901 ccgcttctgc caagatttca aacagtttaa ccgcagtgta gggtgtatct tcagccggct
      961 tgagcaccac cgtgttgcca cagagtagcg ctggaaacaa tttccacgta gggatcgcca
     1021 tgggaaagtt ccatggcgta atcaacgcgc agacaccgat gggcgagcgc atcgacatag
     1081 caaatttgtc cggcagttcc gaaggagtcg tctcgccgaa aagccgtcgc ccctcgccgg
     1141 cgacatagaa ggcagtatcg attccttcct ggacgtcgcc gcgtgtctcc ttcaagatct
     1201 tgcccatctc ccgggtcatg ccgtgagcga tctcttcctt atgccggcgc aaaagctcgc
     1261 ccacccggta gaggatctcg ccccgttttg gcgccggcac gaggcgccag agcgcaaacg
     1321 ccttctttgc ggcctgaacc gctccgttta catcctccgg gctggaggcc ggaaacaagc
     1381 cgatcaagtc gtgacgattc gccgggttat ggttttcaaa ggtcttccct gacttggatc
     1441 ccacccatgc cccatcgatg tagttcctga agacctccgc catggtcaaa cctcctttcg
     1501 gcgccttccg agcgccgggc aattagcttc catgtctttt tcggccgaac cgcagtttat
     1561 tctaaggccg agctgtcatg aacacaatct ttaagtttag ccaaagggga tctttttgga
     1621 ggtcagcggc tgcaaaaatc ccgttgattt ttcaatttca atcagatagc ctctgccaac
     1681 tttggcagcg agatcatcca acagatgctt agcctatgtt caatgagacg ataaggagag
     1741 ctggtgcatg gggaaaaaat tgattctcct agccgtcgcc ttgatgctga gtttcgaaac
     1801 agctagcgcg gcatctgggg atgactttta caaaggcaag acgattagga tcgtggtcgg
     1861 tttttcggcc ggtggcgggt tcgataccta tgcccgcgca ctcgcccgcc atctaagcaa
     1921 gcacattcct ggacaacccg ccattgtcgt ggagaatatg actggcgccg gcagtctcat
     1981 agcggccaat catgtataca aggtcgctaa acccgatggt ctcactatcg gccatttcat
     2041 cggcggactg ttcctcgggc aggttctcgg gcaaaaagga gtcgaattcg acgcgcgcaa
     2101 attcgagtac cttggtgcgc ccattaccga tcacgtcgtc tgcgccatga ccaaagccag
     2161 cggtattacg aacgtagaga cctggatggc gtcaaagaca ccggtcaaaa tggggggtat
     2221 cgcccccggc acatcgacgc cggacaacgc tacgcgcatc ttgaaagcgg ccgtggggct
     2281 gccgatccag cttgttaccg gttacaaagg caccgccgat gtcaggttag ccgcggagag
     2341 cggcgaggtc gccggtggat gttggggttg ggattccgtc agcgtcacct ggaggaaagc
     2401 gttagatagc ggcgacgcag tcgtcgtcct acaggccaat cgccgaaccc atcctgacct
     2461 tccccaagtc ccccaggcaa tcaagctcgc caagaccgac gaaggccgca aaatgatcga
     2521 cgtcggtatc cacagcgaca gcgatattgt tcgcacctac acgcttccac cgggaacccc
     2581 gaaggatcgg gttcaattgt tgcgtaaagc cttcgaggac accctcaagg atcctgagtt
     2641 ccttgcggac gcgaaaaaat ccaaactcaa tgtcgatccg gtaccggccg atgtcatcga
     2701 gaaggacatc tcggctctgt tcaagctcga cccggcaaca gtgggcaaac tcaaggacgt
     2761 tctttacaac tagctccgct aacccatgga ccggcaacat tctgcggatg acatcccgat
     2821 gaaccccttt tgaagatcta ggccgccatg ttggaaatgt tcagcgcatt gttcgccggt
     2881 ctgattcagg tttttaactg gtctactttc agcctgatga taatcgggat cgcagtgggc
     2941 tttgtcgtgg gtattctgcc cggcctcggc ggaccgacgg ccatggcctt gatgttgcct
     3001 ttcattttca agatgtccgc ggtcgaggcc ttcgccttcc tgctcggcat gacggcggtc
     3061 acggcgacga cgggcgacat cacctccgtt ttgtttggag tgccgggcga gcccaccacg
     3121 gcatcgacca ttgtcgatgg ccatgccatg gcgcgaaacg gcgaagcggg tcgggcgctg
     3181 ggagcggtgc tgatgagctc gctggtcgga gcgatattcg ctggcgtcgc gctggctgta
     3241 gccattccgg ttgtacgtcc gatagttctt tcgttcggtt ctcctgagtt ctttatgctg
     3301 tcactgctcg gcatcacctt cgtcgcctca ctgagcggtg aggatcaact caaaggcatt
     3361 gttgcgggcg gaatcggctt gatgcttgcc accatcggtt tagatcccat ttccggaata
     3421 caacgctaca ctttcggcca actgtttctc tgggacggga ttggtctcgt accgatcacg
     3481 atcggcttct atgccattcc cgagaccatc gagctagccg tgttgggaac cagcatcgct
     3541 caggaaaagg tagaaaaaat cggcggagtc atggaaggag ttaaagatac cttccgtcac
     3601 tggtggctgg tgatccgttg tagcgccctc ggtactttca ctgcgatcat tcccggtatg
     3661 ggggccgcga cgacccagtg gctggcctat gcgcacgcgg tccagagctc gcctgacaag
     3721 gagcgctttg gcaaagggga tgtgcgggga gtcctgggtc cgggcgcagc gaataattcg
     3781 acactgggtg ggagtttgat tacgaccatc gcgtttgggg tgccggcgag tgttgtcatg
     3841 gcgattctgt tgggtgcctt catcatccaa ggcatcgtgc ccggacccga catgctcgtt
     3901 cccccgccta agggaaaact cgatctgacc ttttcctttg tttgggtcat cattatctct
     3961 aactttatca ccgtagctgt ctgcttcctt ttcctgaata ctttggcaaa ggtgactcaa
     4021 gtgcgaggga gtctgattat tccgcttatc cttttgctca tctacctagg cgcgttcgcg
     4081 gagaaaaatg ctttccaaga tatgatcgtc gtcctcttct tcggcggcct aggttggatc
     4141 atggaaaaat tgcactggcc gcgtcccccg gtgctgttgg gattggtgtt ggggccttta
     4201 gccgaaaatc gactattcct cgccagcgac aattacggca tggcatggac ctggcggccc
     4261 ggcgttctaa taatctttgc agtgactcta gttggtattc tttatccgct catcaaaaag
     4321 aaaagggagg agaagacaag tcgaaccgcc gcgactagcg tcgaagccac ccccgaaaga
     4381 ggcctgcagt tcggctggcc agttgttttt accgcttgcg ttatcatcct tcttgctttg
     4441 gccctatggc agagcagaaa ttttggggtg cgcgctggat tgtttccctg ggtgatcggc
     4501 acgccgacac ttctgctggc gttgatccag ctcgccaagg atttaagagg tcataggaac
     4561 gtcagcgggc cggcattcga ggccgagggg gctgtcgagg tttcgccgga ggtggctacc
     4621 cgccgcacct tgacgatcct cggttggacc gtgggattct ttgtggcgat ttggctcttg
     4681 ggattttctt acgcggtgcc gttaacgatg gtcctttatc tcaagctcgc cgggagagaa
     4741 aaatggccga ttaccgttat catgaccttt tgtacatggg gttttttcta cctcttgttc
     4801 caaaagatgc tgaacgtacc gttccccgac gggctaattt tcgatttgtt taaagggcag
     4861 tgattcgccg ccggtcccct ccgatatttt ccacgctatc tcagcccgat ttcagctctg
     4921 attctttgcc cgcagcgcgt gccagacgcg ctcgggcgtg agcggcaagc gatatagccg
     4981 cacccccgtc gccttgtaga gggcgttgcc gatcgccggc gccaccggca acaacccccc
     5041 ttctcctgtt cccttcgcgc cgaaaggccc cgggccgttg tgattttcca cgagtatggt
     5101 ttcaaaacca atcggcaagt cgctgaaggt cggaacccgg tagtccacaa gattgggatt
     5161 taagagctgt ccatctcgat agaccatttc ttccagcagg ctgtggccga gcgcgttcat
     5221 cacgccgcct tcatcttgac cctcgcacag caccggatgg atggcctggc ccacatcggc
     5281 cagcgagaca tatttaatca atttaatttc gccggtgtcg cgatcgacct caacttccgc
     5341 tccaccccaa gagatctccc aaaaagtcgt gggagagccc aaggccgcct ttttactttt
     5401 gatgtcttgg taagacccgc gtccgacgat ttcaccggcc ttcgatgaaa aagctttgtg
     5461 catgaggtct tcgaaggaaa tgctttgtcc cttggggcct tcgattcgtc catgcttgag
     5521 tgtcaatcga gtgggagtcg ctttgagcac tttggctcca t
//
LOCUS       DASSWZ010000127         6844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_838736, whole genome shotgun sequence.
ACCESSION   DASSWZ010000127 DASSWZ010000000
VERSION     DASSWZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838736"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VFN58_03175"
     CDS             complement(<1..1433)
                     /locus_tag="VFN58_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8917026.1"
                     /translation="MAKAERFSVVGQRVQRIEGFDKVTGESKFIADIQLPGTLIGRVL
                     RSPYPHARIRHIDTGKAAKLRGVRAVVTAEDTLKRPWGAFFADQYILSVGKARYVGEE
                     VAAVAAIDPDIAEEAIDLIEVDWKPLPGVFDAEEAMKDAAPLVHDDKPNNIAMTMNIE
                     RGNIAQAFAESDVIVEDTFTSMPQWHCSIETIGSVAEYSANGKYTIYMNTQTLFSARL
                     RIAAALGVPESDVRVIQSAVGGGFGGKSCDDNNALVAAVLAKKAGKPVKLINSREEEF
                     LAGCRPRVFMKINVKVGFKKDGRIRAKHIKVIADNGAYSAKAPAITGVAAMRHDTCYK
                     YSDVKTEALLVYTNKIPTGAFRGFGNPSAEWAVEQAIDLGAHELGIDPIEIARINAAE
                     VGYVSPHGNRVTSCELKQCLDMVDKMIDWKGKRANKQPNTGLGLACTVHVSGKRHFGD
                     YDGSSATIKINEDGKALILSGEGECGQG"
     gene            complement(1648..2199)
                     /locus_tag="VFN58_03180"
     CDS             complement(1648..2199)
                     /locus_tag="VFN58_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8917027.1"
                     /translation="MKKPLILTVNGEEHELEIEPNRLLLHVLRDDIGLTGTKEGCSIG
                     VCGACSVIIDGRLVSSCLTLAAGCQGKSIETIEGLAKDGTLHPLQQAFIEYGGFQCGI
                     CTPGQIMAAKALLDENPNPTEDEVKEWMSGNLCRCTGYYKILESVMAVVHNKISPDFQ
                     FRKREQQAKIQSLDVYKSVAKTD"
     gene            complement(2203..3858)
                     /locus_tag="VFN58_03185"
     CDS             complement(2203..3858)
                     /locus_tag="VFN58_03185"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8917028.1"
                     /translation="MNFAKPYNDLREFIAALEEHGKLYRIHREINKDTELQPLVRWQF
                     RGLSEEARKGFLFDNVTDSKKRKYNCSVLVGGLAGSEAIYCLGLKCQPDEVADRWIYA
                     LDHPIEPVIINEGPCQEEIHKGGELLAQGGLNEFAIPISTPGFDNGPYITAGHWITKD
                     PETGKRNVGNYRGLIKGPALSGLMSGTPQDLSTHWEKCRKKGVPLEVAIVVGTVPAVS
                     YAATQKVPPEVDELALAGGLMGEPVKLVKCQTLDLEVPATAEIVLEGVIPTNYMEEEG
                     PFGESMGYVDPRTLSTVFELKCVTHRKNPIWVSIISQVTPSESSKIKAMGMATLIKRF
                     LVNRGLDSLREVHLMEPLVNLRPYVVVSLKKRNDQEPWDAMQGVLDYGDRVGKLIVAV
                     DEDINPRDPVAVTWAITHRSQPHKDIKVIGDRPFGATPIGMVATHPSSRYDNCESSLL
                     IDATRKADFPPLSLPKKEYMVRAREIWEELGLPKLDPQEPWHGYLMGLWPEDLAQEAD
                     LAAQSEHERVWEKLRQTRVQVGEGETLKSMRAKWGKSHSGRAE"
     gene            4157..5977
                     /locus_tag="VFN58_03190"
     CDS             4157..5977
                     /locus_tag="VFN58_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917029.1"
                     /translation="MEDAIRVLRPSRVRLKLLLLSLAIVTEIHAQESTRDRGQNDTGQ
                     LTLQKTAQSREYQGREVEGEDRRVGPGDSIWRILVKEKGLSENRFTQYLVIMRGLNPK
                     IKNIDVLKVGDNLFIPLRPDELLAGPAVPVKTDVQRSTVTKGAIKEYRVKQGEHLYQI
                     IREQLGIANDRDVALYYALVKDLNPERKNWDALLGGDLIRLPVPGKSSEFTVAERKVS
                     IESTPATEPIKEMNPPSAVGQVKEDKPPTPIVLGLDYARRLPARENLALLGQVVEVLG
                     NEVRRDGQETVTLKDGTVRVDRTSYPVVYNPKLRQRIILDPDDKIPASVKSKLTDPSV
                     YTAVFPVTGTASLQESVSQLLSRLGYQSLPTDRPVVIQEGGIAIEAKGNWMALAPEEN
                     NKAQEIFVIALTDNPQDIPDYLRKELATRGLHFKDIPLSAFSSQSPATNESQESPSAI
                     RHWPREKREFIDAVLLAFGVPFGVAETLSTELGHGLRVDVRCDRIFERNGKRTGLFFQ
                     RLEPEIKRILQERDKIKVIELDLAVLENKEIMARVLSELGEQAAYREHRFVASASKDR
                     LNITAWGFLLARRGMFVTDREIPQSLHRFFFEKGLEIVYF"
     gene            6006..6082
                     /locus_tag="VFN58_03195"
     tRNA            6006..6082
                     /locus_tag="VFN58_03195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6040..6042,aa:Arg,seq:tcg)
     gene            complement(6164..>6844)
                     /locus_tag="VFN58_03200"
     CDS             complement(6164..>6844)
                     /locus_tag="VFN58_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET8917030.1"
                     /translation="RKKIDPLTREEIQVMLKAASEKKYIHWYPLLLCAPRTGIREGEL
                     VTLKGADVDFNGRFIHVQRNLSRGKISATKNGKDRKVDMSAQLADVLSDVLSKRRAEA
                     LRREMEKPAEERQDAATVVNEVMESWLFQTPVGTQIDPSNLRKLFNRLLTDAKLRRIR
                     FHDLRHTFASLLLQNGESPAYVKEQMGHSSIQVTVDIYGHLVPGGNRQAVDRLDDAPT
                     STKKAATA"
BASE COUNT         1502 a         1789 c         1836 g         1717 t
ORIGIN      
        1 ccctggccgc attcaccctc gccacttaaa attaaagcct tgccgtcttc attgattttt
       61 atcgtcgcgg agctgccgtc ataatcgccg aaatgacgtt tgccgctgac atgaaccgta
      121 cacgccaagc ctaatccggt attcggctgt ttgttggcgc gcttgccttt ccagtcgatc
      181 attttatcga ccatgtcgag acactgcttg agctcgcagc tcgtgactcg gttgccgtga
      241 ggggagacat acccgacttc agctgcgttg atgcgggcga tctcgatggg atcgatgccc
      301 agttcgtgag cgccgagatc gatcgcttgc tcgaccgccc attcagccga tggattgccg
      361 aagccgcgaa acgcgccggt gggaatcttg tttgtataaa ccaacagcgc ttcggtctta
      421 acgtccgaat atttgtagca agtgtcgtgg cgcatcgcgg cgactccggt gatcgcagga
      481 gctttagctg agtatgcgcc gttgtcggcg atcactttga tatgcttggc gcgaatacgg
      541 ccatccttct tgaaacccac cttgacgttg atcttcatga agacacgcgg ccggcagccg
      601 gcgagaaact cttcttcgcg gctattgatc agttttaccg gcttgccggc tttttttgcg
      661 aggaccgcgg caaccaaggc gttgttgtcg tcgcaggatt tgccgccgaa tccgccgccc
      721 acagcgcttt ggatgacgcg tacatccgat tccggcacgc ccagcgccgc ggcgattcga
      781 aggcgcgcgc taaacagcgt ctgggtgttc atgtagatcg tgtatttgcc gttggcgcta
      841 tattccgcga cgctgccgat ggtttcgatc gagcagtgcc actgtggcat actcgtaaaa
      901 gtgtcttcga cgattacgtc ggactcggcg aaagcttggg cgatgttgcc gcgctcgatg
      961 ttcatggtca tcgcgatatt atttggcttg tcatcatgca cgagtggcgc ggcgtctttc
     1021 attgcctcct cggcgtcaaa gacaccagga agaggtttcc aatccacttc aatgagatcg
     1081 atggcctcct cggcgatatc ggggtcgatg gccgccacgg cggcgacttc ctcgcccacg
     1141 taacgcgcct tgccgacgga tagaatatac tggtccgcga agaaagctcc ccagggacgt
     1201 ttgagggtgt cttccgccgt gaccacggcg cgcacgccgc gaagctttgc cgctttgccg
     1261 gtatcgatgt ggcggatgcg cgcgtgcgga taggggcttc ttaaaactcg cccgataagc
     1321 gtgccgggta gttggatatc agcaataaac ttcgattcgc cggtaacttt atcgaaacct
     1381 tcgatccgct gaacacgttg cccgacaacg gaaaatcttt ctgcttttgc catgatcagt
     1441 gttctctgcg atttctaatt gagtgtcttt gcctcaatct gcgccggctc gctgcgtaag
     1501 cgagattttc tgttcttctc gagtccgtca tgcccgcatg cttttagcgg gaatccaggc
     1561 caaacgctga ctggccgccc gattaaaaca ttcgagggtg acgatacttc gtctccgcta
     1621 tggtgagcca aaattctctt gcaaagctca atccgtcttc gcgacagact tgtagacgtc
     1681 gaggctttgg atcttcgctt gttgctcgcg ttttctaaat tgaaagtctg ggctgatctt
     1741 attgtggacc accgccatca ccgattcgag aatcttgtag tagccagtgc aacggcagag
     1801 gttgcctgac atccattctt tgacctcatc ctcggtggga ttggggttct cgtcgagcag
     1861 cgccttggcg gccatgatct gtcccggcgt gcagataccg cactggaatc ccccgtactc
     1921 gataaaagct tgctgcaacg gatggagcgt accatccttt gccagcccct cgatcgtctc
     1981 aatgctcttg ccttgacagc ccgcagcgag ggttaagcat gagctcacga ggcggccgtc
     2041 gatgatcaca ctgcaagcgc cacagactcc gatgctgcaa ccttccttgg tgccggtcag
     2101 gccgatgtcg tcccgcaata cgtgaagcag caggcgattg ggctcgattt cgagctcgtg
     2161 ttcttcgccg ttaacggtca ggattaacgg ttttttcatg acctattccg ctcttcctga
     2221 atgggatttt ccccatttcg ctctcatcga cttcagcgtt tctccttcgc caacttgcac
     2281 tcgagtttgg cgcagttttt cccagaccct ttcgtgttcg ctttgagcgg ctaggtcggc
     2341 ttcctgagcc agatcttcag gccagagtcc catgaggtaa ccgtgccaag gttcctgggg
     2401 atcgagtttg ggaagaccga gctcttccca gatttcccgc gcgcggacca tgtattcctt
     2461 ttttggcaac gaaaggggag ggaagtcggc cttccgcgtt gcatcgatca aaagcgatga
     2521 ttcgcagttg tcgtagcggc tggaaggatg agttgccacc atcccgatgg gtgttgcccc
     2581 aaagggccga tcgccaatca ccttgatatc cttgtgtggt tgcgaacggt gggtgatcgc
     2641 ccaggtcacc gcaacagggt cgcggggatt gatgtcctca tccacagcaa cgatcagctt
     2701 gcccacccga tcgccataat caagaacacc ctgcatagcg tcccacggtt cctgatcatt
     2761 gcgtttcttc aaactgacga cgacgtacgg tcggagatta accagaggct ccatcagatg
     2821 gacctcgcgt aaggaatcca gcccacggtt gacgagaaag cgcttgatca aagtagccat
     2881 gcccatcgct ttaattttgg agctttcgct gggtgtgacc tggctgatga tcgaaaccca
     2941 tatggggttt tttctgtgcg tgacacactt caattcgaaa acggtgctca gtgtgcgcgg
     3001 atcgacatag cccatagact cgccgaaggg accttcctcc tccatgtagt tggtcgggat
     3061 gactccctct aagacgatct cagcggtcgc gggcacttcg agatcaaggg tttggcattt
     3121 taccagcttc actggttcgc ccatcaggcc acccgcaaga gccaattcgt ctacttccgg
     3181 cgggactttc tgggtggctg cgtaggagac cgctggcacc gtgccgacaa cgattgcgac
     3241 ctccaaggga accccttttt tgcgacactt ctcccaatgc gtcgaaagat cttgcggcgt
     3301 gccggacatg agaccactaa gcgcaggtcc tttaatcaaa ccgcgataat tgccgacgtt
     3361 tcttttaccg gtttccggat ctttggtgat ccaatggccg gcggtgatgt acgggccgtt
     3421 gtcgaaaccc ggggtcgaaa tcgggatggc aaattcgttg agaccgcctt gcgcgagcaa
     3481 ttcaccgcct ttgtgaatct cctcttggca cggtccttca ttgataataa cgggttctat
     3541 cggatgatcg agagcgtaga tccaacgatc ggcgacttca tccggctggc acttaagccc
     3601 caggcaataa attgcttcgg aaccggcgag gccgcccacc aacacggagc agttgtattt
     3661 tcgctttttc gaatcggtca cattatcgaa caggaatcct ttgcgggctt cttccgatag
     3721 tcctctgaac tgccatcgga ccagcggctg caactcggtg tctttgttga tctcccgatg
     3781 gattcggtaa agtttcccgt gctcttccag agccgcgata aattctcgta agtcgttata
     3841 aggtttagcg aaattcatag gattttgtct atgctgtttt gccttttttg gcaagcggtg
     3901 aaccaaaaat agcttgcaaa acaccgtgtg gaccctaaaa ttgaaaagtt ggcaatacgt
     3961 tgctcaagat tttcaacccg ctcagtggca aaaaagagga tttttttccg ctaatggaaa
     4021 gcctaaccgg gtgtggtctg caacgtaacg atgtatgaga gtcaaaaagc tagaaacctg
     4081 tagaagccga tgcgatttcg ctagtaacaa agaggataaa gaaatttttg tttgaagagg
     4141 ccctcggagg aaccgaatgg aagatgcaat tagagtcctg cgtcctagca gggttcgcct
     4201 aaagttattg ttgttgtcgc tggccattgt gacagaaatt catgcgcagg aatctacacg
     4261 cgatcgtggc caaaacgata ctggccagtt gacgttacaa aagacagccc aatcgcgcga
     4321 atatcagggg cgtgaagtgg agggcgaaga taggcgtgtc gggcccggtg attcaatttg
     4381 gcggattctg gtgaaagaga aaggactttc ggaaaatcgc tttacccagt acctggtaat
     4441 catgcgggga ctcaacccaa aaattaagaa catagatgtg ctcaaagtcg gcgacaatct
     4501 ctttattcct ctgcgtcccg atgagttact cgcaggccca gcggtgccgg tgaagacaga
     4561 tgtccagcgc tcgaccgtta ccaagggagc catcaaagag tatcgcgtga agcagggtga
     4621 gcatctctac cagataatca gggaacagtt ggggatcgcg aatgatcgtg acgtggcatt
     4681 gtattacgca ctagtgaaag acctcaatcc ggaaagaaaa aactgggatg cccttctagg
     4741 gggtgattta atccggctgc ctgtgccggg caagtcgagc gagtttacag tagctgaacg
     4801 gaaagtttca attgaatcaa caccagcgac cgaaccgatc aaggaaatga atccaccgtc
     4861 ggctgtgggg caagtcaaag aagataagcc tcccacgcct atagtgctag gcctggatta
     4921 tgcgagacga ctgccggcta gagagaacct cgcgttactg ggacaggtgg tcgaagtctt
     4981 gggaaatgaa gtccgacgcg acggccagga aaccgtaact ctgaaagatg gcacagtgcg
     5041 agtcgataga acttcttatc cggtcgtgta caacccgaag cttcgccaaa ggatcattct
     5101 ggaccctgac gataagattc ccgcctccgt aaaatcaaag cttacggatc caagcgttta
     5161 cacggcggtt ttcccggtca cgggaaccgc atccctccag gagtccgtca gtcaactgct
     5221 ttcgcgtctg ggatatcaat ctttgccaac agatcggccg gtggtgattc aagaaggagg
     5281 aattgccatt gaagctaagg gtaactggat ggcgttagcg ccggaggaga acaataaagc
     5341 tcaagagatt tttgttattg cgctgaccga taatccgcaa gatattcctg actatttgcg
     5401 aaaggagttg gcgacgaggg gactgcactt caaagatatt ccattgtctg ctttctccag
     5461 ccagtccccg gctaccaatg agtcgcagga gtccccgtcg gcgataagac attggccgcg
     5521 ggaaaagaga gaatttatcg atgccgtgtt attggccttc ggcgttccct tcggtgttgc
     5581 cgaaacgctt tccaccgaat tgggacacgg gcttcgggtc gacgtacgtt gcgatagaat
     5641 ctttgagcga aatggcaagc gtacagggtt gttctttcag cgtctggagc cggaaataaa
     5701 aaggatcctt caggaaagag ataaaattaa agtcatcgag ctggatctgg ctgttttgga
     5761 aaacaaggaa atcatggcgc gtgtgttgag cgaactggga gaacaggcag cctatcggga
     5821 acatcggttt gtcgccagcg cgagcaaaga tcgattgaat attactgcct ggggtttttt
     5881 gctggcaaga cgaggcatgt ttgttacgga tcgggagatt ccgcaatcac ttcaccggtt
     5941 cttttttgaa aagggattgg agatagttta tttttagctg gccagattgt agactccgtc
     6001 aagctgcgcc cgtagctcaa ctggacagag catcagactt cggatctgag ggttgggggt
     6061 tcaaatccct ccgggcgcgc caatgatttc agcggcttac acccctcaaa tctgattttc
     6121 tctccaattg tttccacttt tgtttccact cttgtctttt tgcctatgcc gtggctgcct
     6181 tttttgtgct ggttggcgca tcatcaagcc tatcaacggc ttgcctgttt ccaccgggta
     6241 ctaaatggcc gtagatatcc acggtcacct ggatggaaga atgtcccatc tgttctttga
     6301 cgtatgccgg gctctcgccg ttttggagta acaagctggc aaaggtatgt ctcaggtcat
     6361 ggaacctgat gcgcctgagc ttcgcgtcgg taagaagtcg gttaaagagt ttgcgcagat
     6421 tactaggatc aatctgtgtg ccgaccgggg tctgaaacag ccagctctcc atcacttcat
     6481 tgactaccgt ggcagcatct tggcgctctt ccgctggctt ttccatttcc ctccgaaggg
     6541 cctcggcccg gcgcttagac aggacatcgc tcaacacgtc agcaagctgc gccgacatat
     6601 caaccttacg atccttgccg ttcttagtag cgctgatctt ccctctggac aggtttcttt
     6661 gcacatggat gaaacggcca ttgaagtcca catcggcgcc tttcagtgtt accagctcgc
     6721 cttccctgat gccggtacgc ggagcacaca agagtagggg ataccagtgg atatattttt
     6781 tctcactggc ggctttgagc atcacttgta tctcttccct tgtcagcggg tctattttct
     6841 ttcg
//
LOCUS       DASSWZ010000128         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_329439, whole genome shotgun sequence.
ACCESSION   DASSWZ010000128 DASSWZ010000000
VERSION     DASSWZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329439"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1380
                     /locus_tag="VFN58_03205"
                     /pseudo
     CDS             431..1380
                     /locus_tag="VFN58_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(1459..2199)
                     /gene="surE"
                     /locus_tag="VFN58_03210"
     CDS             complement(1459..2199)
                     /gene="surE"
                     /locus_tag="VFN58_03210"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET8917031.1"
                     /translation="MRILIANDDGIYSPGIATLAEAATEFGEVRVVAPDVEQSSAGHS
                     ITALRPLSYKRTPIGNFEAYRVNGTPADCVALGAYHWEKVDVVLSGINLGSNLGHAMW
                     HSGTLAAAKQAALLGLRGIAFSAAVTRDEPNLEVLKTSLEEVLALLLKERDLSLVNVN
                     FPDRKPKGLRWTRQSVRRYDGKVVPGEHPLGRKLFWFTVVPIEGTDEGTDRWAVEHGY
                     VSMTPLRLDLTNEEELARAQLKHPLSNA"
     gene            2662..>3920
                     /locus_tag="VFN58_03215"
     CDS             2662..>3920
                     /locus_tag="VFN58_03215"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HET8917032.1"
                     /translation="MIKDESTLIDRDLSWLEFNRRVLEEAKEPSVPLLERVKFLAIFS
                     SNLDEFFMVRVAGLKRRANNSGTASDDVTGASALTAISQKVHELAEEQHLCFLDTILP
                     QLTAEGIHLLRPEEISREQEQFLEDYFRKTLYPIVTPLAIDPGHPFPYLANRSLCLVV
                     SLQAKDTSPLPHTELSIVHIPGQIVRRFIQLPSKDGQYAFVLLEDVLQQYLPRLYHGF
                     DLLSTYAIRVTRDAEFGVVRRRNEDLLMTIEQGIRERRMGDAVRLQYDPDLPKELLTQ
                     LMEELELTGEDLYPARGFTAFTDLFQLYTTVNVPRLKDRIQPALSLPSFDRGPDIWNA
                     IRNGDVMIVHPYQSFDPVTHFVEEAATDPKVLAIKTTLYRVSPNSPIVQALLRAAEAG
                     KEVSVLVELQARFDEEANITWARALEEV"
BASE COUNT          872 a         1077 c         1040 g          931 t
ORIGIN      
        1 ggattagtca tctatattga gatgaagcac gacacgtagt ctgtattttc cgacagagtg
       61 gaaagttttg agcggcatgc ttggttcccg attcaaatgt cgggaatttt tttccgctgc
      121 gatgttccgc gtgacatcag agctccccgt aacttcggct ggttcattcg atttagtgcg
      181 ctttagtggc acccgctttg cacttttaca tcttgggtaa tgcggttgat cgcccgaatt
      241 gcgggctcaa gctgccattg gtaattgatc gattcgttcg agaagcaaaa acgagcagag
      301 aacggagttg cttagtgaag aaaggaatat tcttatgaat gcacgaccct cgtcctctgt
      361 tactttcagg ttggctctcg ccggcgccgc cgctgctacc ttagtttatg cagtgcggca
      421 acggcggcgt atggacttcg cggcaaaggt cgtggtgatt accggggctt cccgcggcct
      481 cgggctggaa ctggctcgac aattcgccaa ggaaagggcc catgtcgtgc tgctggcacg
      541 ccatcgcgaa caactcgccg aggtggcgcg ggaattggaa tccatcggcg ctcgggtgac
      601 ggtgctgcaa tgcgatgtca ccaaggaaga cggagtccgg gcgggatggc atcgattatc
      661 cgagatctgg gacgagtcga tgtcttgatc aacaatgcag ggataatcca agtgggtccg
      721 gcgcagcaca tgaataacga tgactttacc gaagcgatgg cggttcactt ttgggcgccg
      781 ctttatttga tgcgtgcggt cattccgcag atgaaacgtc aaggccatgg tcgcatcgtc
      841 aacatcgcat ccatcggcgc taaaatcgcc tttccccact tactgcccta tacagccagc
      901 aagtttgcgc tggctggtct ttcggaggga ctgcgcacag agcttgcgca agacggtatt
      961 tacgttacaa cggtctctcc gggattgatg cgcaccggct cccatgtcaa cgcccttttc
     1021 aaaggccagc agcgaaaaga gttcgcgctc ttcagcgccg ccaatgcctg gatttcgacc
     1081 agctcgaccc gggctgcgcg ccagattgtc gaagcatgcc gctacggttc gagcgaattg
     1141 gtaatcactc cccaggcgaa gttgctgcac ctgctaaacg gcgtccttcc caatttggtc
     1201 gccgaggctc tcagcctact ctcccgggta ttgccggaac cggcgggcgc tgagggcgac
     1261 cagctgaagc gtggatggga aagccaatcg gttatggcac cttccgtatt gactcggcca
     1321 gcggaccgcg cgacagacga gaacaatgag gaactgcaac cggccctggc aaaatattaa
     1381 ggagttctaa gtggctaggc gggaaataaa tatctttgga aggacaactc acgatggcgt
     1441 ctaaactcgc ctgaagaact acgcgttact caatggatgt tttagctggg cacgggccaa
     1501 ttcttcttcg ttcgtgagat cgagccgcaa cggggtcatt gaaacgtagc catgctcaac
     1561 ggcccaccga tccgtaccct cgtccgtccc ctcgatagga acaacagtga accagaaaag
     1621 tttgcgtccc aacgggtgtt cccccggtac tactttgccg tcatagcggc gcaccgattg
     1681 acgcgtccag cgcagacctt ttggctttcg atctggaaag ttcacattga cgaggctcaa
     1741 gtcgcgctcc tttaacagta acgctaaaac ttcttccaac gacgttttca aaacctctaa
     1801 gttcggctcg tcccgggtca cggccgcgct gaaagctatg ccacgcaatc ccaatagagc
     1861 tgcctgtttg gcggcggcca acgtgcctga atgccacatc gcgtgcccga gattactacc
     1921 cagattgatg cctgaaagga ccacgtccac cttttcccag tggtaagcac ccagcgcaac
     1981 acagtctgcg ggcgtgccgt tgacgcggta agcttcaaaa ttcccgattg gagtgcgctt
     2041 ataagagagc ggccgcaagg ctgtgatcga atgtccggcc gacgattgtt cgacgtccgg
     2101 agcaacaacc cgcacttcgc cgaattctgt cgcggcctcg gcaagggtcg cgattccggg
     2161 gctgtagata ccatcatcat tggcgatcag aatgcgcatc agaccagtct cctctcgatt
     2221 acacgtcaac ctgttattct ctccatcccc cgcagtacca ttgcgtttat agaagttaga
     2281 tgaagggagt cacgcagacc tttcggttca acgtcgtttg cgtggcaatt ccaataacga
     2341 acgcaaattt tgcgcccgtt ctacttgagc ctgtgaatga taaccggcgc gtgcattcct
     2401 tgttctttca ttgagcgtgt aggtacttgg cttacggtcc gagccgggtt tgtgcgtaaa
     2461 tgatgcacaa caccggctaa ccgcaccgac aagctcctcc tccgccctgg catgtacttt
     2521 gcgcagctag tcggcgtatt tcgccatcgt gcttactgaa gcttgctaaa cgaaaacgat
     2581 tctattcccg ggatcggact gtttgttagt aagatcaaac tgaaaagacg cgtggtatga
     2641 atttattgat aggccgaggc catgataaaa gatgaatcga cgctgattga ccgcgacctt
     2701 tcctggcttg aattcaatcg acgtgttttg gaagaagcca aagaaccctc tgtcccgctc
     2761 cttgagcggg tcaagtttct cgccatcttc agctccaatc ttgatgaatt cttcatggtc
     2821 cgggtggctg gtctgaagcg ccgtgccaat aacagcggga cagcttcaga cgatgttacc
     2881 ggggcgtcgg cattgacagc gatctcccaa aaagtccatg agcttgcgga agaacaacat
     2941 ctctgtttcc tcgatactat tttgccgcag cttacagccg aaggcattca tttgcttcgg
     3001 ccggaggaaa tcagccgcga gcaagaacaa tttctggaag actactttcg caagacgctt
     3061 tacccgatag taactccgct agccatcgat ccgggtcatc cgttccccta tctcgccaac
     3121 cgttcccttt gtttggtggt atctctgcag gcgaaggaca cctcgccttt gcctcacacg
     3181 gaactttcta tcgttcatat tccgggtcaa attgttcggc ggttcattca gcttccgagc
     3241 aaggacgggc aatacgcatt cgtgttgctg gaagacgtcc tccagcagta tctgccgcgt
     3301 ctctatcatg gttttgacct gctctctacg tatgccattc gggttacgcg agacgctgaa
     3361 ttcggcgttg tccggcgccg caatgaggat ttgctgatga ccatcgaaca gggaatccga
     3421 gagcgccgta tgggtgatgc cgtccgtttg caatatgatc cggaccttcc caaagaactg
     3481 ttaacccagc tcatggagga actcgaactg accggagagg atctttaccc agccaggggt
     3541 ttcaccgcct ttactgattt gttccaactc tataccaccg tgaacgttcc cagactgaaa
     3601 gaccgcattc agccggcgtt atcgctgccg tcattcgacc gcggtcccga tatttggaat
     3661 gccatcagaa acggggatgt aatgatcgtt cacccctatc agtcatttga cccggtcact
     3721 cactttgtag aagaggcggc cacagacccc aaggtcttgg cgatcaaaac gaccctttat
     3781 cgcgtaagcc cgaattcacc gatcgtccaa gcgctattgc gcgcggccga agccggcaaa
     3841 gaggtatcgg tcttggtgga acttcaggcc cgtttcgatg aggaggcgaa tatcacgtgg
     3901 gccagagcgc tggaagaagt
//
LOCUS       DASSWZ010000129         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1121864, whole genome shotgun sequence.
ACCESSION   DASSWZ010000129 DASSWZ010000000
VERSION     DASSWZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121864"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFN58_03220"
     CDS             <1..298
                     /locus_tag="VFN58_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917033.1"
                     /translation="RVLRAYYQAVQETRNDRNSAVKILAKYVRVDDPEILAEVYRIYG
                     VTHLQKNLAVDPEGVKGLLNGLGPEAAQVSPGAFIDVSLLQDLDREGFFQTKNR"
     gene            606..2147
                     /locus_tag="VFN58_03225"
     CDS             606..2147
                     /locus_tag="VFN58_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8917034.1"
                     /translation="MYNRYFGFLESPFSVTPDPRFFYTNPVYQEAYATLRYGIEAKKG
                     FIVITGEVGTGKTTLLRKLLRNLEDTVHSVFIFNTRLTFPELLQVTLHDLGLAPKDTT
                     KVTLLQELNDYLIKQLKQGHTVAALIDEAQNLSDEVLEDLRLLSNLETDREKLLQIVL
                     VGQPELEARLDRPGLRQLKQRVAVQCQLNPLKDEEVGPYIDSRLRVVGYEGKGLFHRD
                     AVQQIALYSKGIPRLINIICDNALLSAYAESKKIVSTDMIKEVATDLRIRSELQVTNA
                     ASAPAVSLPKTERQVLIHKIPDQVSEPKLRRLVSAGIGTLLGILAFVALASIIDPQNL
                     LNTAGSSVEVVKHNPNQETVPKKAIAEVAFKRQDQRITIQYGATIYQIAIDAYGANTA
                     LGMDLIKEFNPQIEDLNWVSAGQDLLLPSLTRETLLRQEPDGSYRLIVGSFRRRTGAD
                     DYARLLSKKGYEVSISPRRVADNLLLHRVEIDGLKNLEEANQIWQTGLRNEWLAFAGN
                     PGGTK"
BASE COUNT          575 a          589 c          553 g          523 t
ORIGIN      
        1 gcgcgtcctg cgcgcctatt accaagcggt tcaagagaca cgcaacgatc gcaactcggc
       61 tgtaaaaatt ctagcgaaat atgttcgcgt cgacgatccg gagatcctcg ccgaagttta
      121 ccgtatctat ggagtcaccc atttgcagaa aaatctcgcc gttgatcccg aaggcgtcaa
      181 gggcctgctg aatggtttgg gacccgaagc ggcgcaagtt agtcccggcg ctttcataga
      241 tgtttcgctg ctacaggatc tcgaccgcga aggattcttt caaacaaaga atcggtagaa
      301 ggcgactagc cagggcgatc gtcctagctc tccgttcctc cgaccacctt tttccagctc
      361 gtagcgctta ctcacctctg actaggttgt aacaataagt caaagattcg caacttttgt
      421 ctaacggttt ccatcttgcg tcgcatctct cgaatttcgg actcgaacaa tatcaattaa
      481 attcttgtaa gtttcttgac tcggctcgat ttgcgaagta agccgatgct agaagttaga
      541 tccgtcccgc tgtgtagaca aagcgaacga ctgcattagc tgctgggaaa aagaggatcg
      601 acatcatgta caaccggtac tttgggttcc tcgaatcgcc ctttagtgtt acccctgatc
      661 cgcgtttctt ctacaccaat cctgtctacc aggaagccta cgctaccttg cgctatggaa
      721 tcgaagccaa aaaaggcttc attgtgatta ccggggaggt gggaaccgga aaaaccaccc
      781 tgctgcgaaa attgttgcgc aacctggagg acactgtcca ttcggtcttt atctttaaca
      841 cccgcttaac ttttccggaa ttgctccaag ttacccttca cgatcttggc ttagccccca
      901 aagacacaac caaagtaacg ctgcttcaag agctgaatga ttatttgatc aagcagctca
      961 aacagggcca tactgtcgca gcgctgatcg acgaagccca aaacctaagc gatgaggttc
     1021 tggaagactt aagactcctc tcgaatctgg agaccgatcg agaaaagctg ctccagattg
     1081 tgctggtggg tcaacctgag cttgaggcaa gacttgatcg gcccggtctg cggcagctca
     1141 agcagagggt ggccgttcaa tgccagctga atcccctgaa ggacgaggag gtgggtccgt
     1201 atattgactc taggcttcgg gttgtcggct acgagggtaa gggtctgttc catcgtgacg
     1261 ctgttcagca gatcgcactc tattcgaaag ggattccccg gctgataaac atcatctgcg
     1321 ataatgcgct actcagcgcc tatgcagagt ctaaaaaaat tgtttccaca gatatgatca
     1381 aggaagtggc cacggattta aggataagat cggaacttca ggttacgaat gcggcatccg
     1441 ctcctgccgt ctcactgcct aagaccgaac ggcaagtact catccacaaa atacccgacc
     1501 aggtttcaga gcccaaactg agacgcctgg taagcgccgg aattgggact cttttaggaa
     1561 tcctcgcttt tgttgccctg gcttccatta tcgatccgca aaatttgctc aacactgccg
     1621 ggagcagtgt ggaagtcgtc aagcacaatc caaaccagga aaccgtcccg aaaaaagcca
     1681 ttgccgaagt cgcttttaag cggcaggacc agcggatcac gattcaatac ggcgctacga
     1741 tctaccaaat tgccattgac gcttatggcg ctaacaccgc gctcggcatg gacctcatca
     1801 aagagttcaa tcctcaaatt gaggatctta attgggtgtc cgcagggcaa gatctcttgc
     1861 tgccttccct aacgcgcgag acactgctgc ggcaggagcc cgacggttcg tatcgtctca
     1921 tcgtgggatc atttcgtagg cgaaccggag ccgatgatta cgcccgcctc ctgagcaaaa
     1981 aaggttatga agtctcgatt tcaccgagaa gggttgcaga caatcttttg ctgcatcgcg
     2041 ttgaaattga cggtttgaaa aaccttgaag aggccaatca gatctggcaa acgggactta
     2101 gaaacgaatg gctcgcattc gccggcaacc caggggggac caaataaaat caagcgcctt
     2161 tgcgcttctt gatctcttcg acgagctgag gaacaacttt gaagacgtcg ccgacgacgc
     2221 cgaagtcgga gtactgaaag
//
LOCUS       DASSWZ010000130         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_329580, whole genome shotgun sequence.
ACCESSION   DASSWZ010000130 DASSWZ010000000
VERSION     DASSWZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329580"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VFN58_03230"
     CDS             complement(<1..733)
                     /locus_tag="VFN58_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET8917035.1"
                     /translation="MDRLTATMSDAENNSPETSLEQVEVRRQKLAKLKEAGHSPYPND
                     FRPSHTTRDILADFSNLSDQELAGLTSEFQVAGRIMGMRKMGKASFFHIQDRRGRIQV
                     YARKDRLGDEGYALLQSLDVGDIVGIWGRLFRTKTKELTIEAQALRMLTKCLRPLPEK
                     WHGLADVEARYRQRYVDLMVNPEVRDLFEKRSRIVRLLRRFFEERDFLEVETPMMQAV
                     PGGAAARPFVTHHNALSMDLYLRVAP"
     gene            complement(744..1607)
                     /gene="metF"
                     /locus_tag="VFN58_03235"
     CDS             complement(744..1607)
                     /gene="metF"
                     /locus_tag="VFN58_03235"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET8917036.1"
                     /translation="MKLADLYARPELTLSVEFFPPKTDKGEENLFSEIEIIKRLNPAF
                     CSVTYGAGGSTREKTVELVDRIHRKCGLEVMCHLTVVGQSKDEARSVLKKLKEKGIEN
                     MIALGGDPPQGMVDWKPHPDGFHHAVELVREAAAFGGFSIAVAGFPEVHPRAESRASD
                     LKYLKEKVDAGAVAIITQLFFDNEEYYRYVEDVRKLGVRVPIVPGILPILSASQIRRF
                     TSLCCAKIPAQLERELIKVENDDEGAVQLGIDYATRQCEGLIKFGVPGLHFYSLNKSY
                     SLQAICKNLNL"
     gene            complement(1715..2125)
                     /locus_tag="VFN58_03240"
     CDS             complement(1715..2125)
                     /locus_tag="VFN58_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HET8917037.1"
                     /translation="MDQTVFEQLGGEPKLRTIIDTFIDRVFDDRMIGFFFRNADRQRI
                     KDMEYQLTAHFLGAGVEYQGRPLDQVHAKHPIMGGHFARRQQILKETLDLYHVPEPIK
                     EAWLTHTDALRPLITSQKDSECDPNALAQKVKSR"
     gene            complement(2189..2509)
                     /locus_tag="VFN58_03245"
     CDS             complement(2189..2509)
                     /locus_tag="VFN58_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HET8917038.1"
                     /translation="MSSINNLVMEEQVLEALRTCYDPEIPVNIYELGLVYEVNVDDEG
                     VVSIKMTLTSPHCPAVQSLPAEIENKVRAVSGVTDVKLDLVWEPPWDPSKMSEAARLQ
                     LGMM"
     gene            complement(2535..3125)
                     /gene="sufT"
                     /locus_tag="VFN58_03250"
     CDS             complement(2535..3125)
                     /gene="sufT"
                     /locus_tag="VFN58_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HET8917039.1"
                     /translation="MSEIEREVVAFMNSEKSIVVKRDCPAVMVPSGETTTLPAGSSVW
                     LTQSLGGAYTVMTDRGYMVRIDGIDGDAIGMAQVAELKQNESTTIENDGEVEKRVWDQ
                     LRSCFDPEIPVNIVELGLIYHCEVTPLSGGGHKATVRFTLTAQGCGMGQFLKADMQKK
                     LLSVAGVREADIEIVWDPPWNQSMISGAAKLQLGIS"
     gene            complement(3122..3580)
                     /locus_tag="VFN58_03255"
     CDS             complement(3122..3580)
                     /locus_tag="VFN58_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET8917040.1"
                     /translation="MSELDELYQEVILDHNRSPRNYRVMANANRKAQGYNPLCGDHVT
                     VYLNLEEGVIKDISFQGSGCAISKASASIMTAELKGKTETEAKELFDNVHKMLTGEAD
                     KNGPQGGRGVGKLTILSGVCKFPARVKCASLAWHTVNSALKGEKSVASTE"
     gene            complement(3580..>3732)
                     /locus_tag="VFN58_03260"
     CDS             complement(3580..>3732)
                     /locus_tag="VFN58_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8917041.1"
                     /translation="AVRAGHHCAMPVMQRFGVPATTRASFALYNTREEIDVLVRGIHR
                     ALKVFK"
BASE COUNT          844 a         1072 c          914 g          902 t
ORIGIN      
        1 cgggcgccac gcgcagatac agatccatac tcagggcgtt gtgatgcgta acaaaaggac
       61 gcgccgccgc tcccccgggc accgcctgca tcatcggcgt ttccacttcc aagaaatccc
      121 gctcctcgaa aaagcgccga aggagacgca cgatgcgcga gcgtttctcg aatagatctc
      181 tcacttccgg attcaccatc aaatcgacgt accgttggcg atagcgcgcc tcaacatccg
      241 caaggccgtg ccacttttcg ggcaagggtc gcaggcactt ggtcagcatc cgcagcgcct
      301 gggcttcgat cgtcaactcc ttggtcttcg tgcgaaagag gcggccccag attccgacaa
      361 tgtcgcctac atccaaggac tgaagcaaag cataaccctc atctccgagc cggtccttgc
      421 gtgcgtacac ctggatccgt ccccgccggt cctggatgtg aaaaaacgag gccttcccca
      481 ttttgcgcat tcccatgatc cggcccgcaa cctggaactc ggaggtcaaa cccgccaact
      541 cttggtcgct caaattgctg aagtcagcga gaatatctcg ggtcgtatga cttggtcgaa
      601 aatcgttggg gtacggagaa tggcccgcct ctttgagttt ggcgagcttt tgccgacgca
      661 cctcaacttg ctcaagagag gtttccggcg aattattttc tgcgtccgac atcgttgctg
      721 tcaatcgatc catctgcgca aacctataag ttgaggtttt tgcaaatggc ttggagggaa
      781 taggatttgt tgagcgaata gaagtgcaac cccggaacgc cgaatttgat caatccttca
      841 cactgccggg ttgcatagtc gatgcctagt tgaactgccc cttcgtcgtc gttttcaacc
      901 ttgatcaact cccgttccag ttgcgccggg atcttcgcgc agcagaggga cgtgaaacga
      961 cggatctgtg atgcggaaag aatcggcaaa atccccggaa cgatcggcac acggactcct
     1021 agttttctca catcctcgac atagcgatag tattcctcat tgtcaaaaaa gagttgggta
     1081 ataatcgcaa cggcaccggc gtcgactttc tccttaagat atttgagatc ggaggcccga
     1141 ctctccgccc tggggtgaac ttcaggaaag cccgccacgg caatggagaa accaccaaac
     1201 gctgccgctt cgcgtacgag ctccaccgcg tggtgaaacc cgtctggatg aggcttccag
     1261 tccaccatcc cttggggcgg atcgcccccg agcgcgatca tattctcgat tcccttctct
     1321 ttcagcttct tgagcacgga tctagcctcg tccttggatt ggccgaccac cgttaagtgg
     1381 cacataacct ccaacccgca tttccggtgg atccggtcga ccagctcaac ggttttttcg
     1441 cgcgtgcttc cgcccgcccc gtacgtgaca gagcaaaagg cagggttcag cctcttgatg
     1501 atttcgattt cgctaaagag gttttcctcg cctttgtctg tctttggagg aaagaattca
     1561 accgataggg tcaactcggg ccgggcataa aggtcggcta atttcacgca ggcgaatcct
     1621 tgatgacaac cttcggcgga tactaattaa taacacaatt ttatcgtagg tcacaaacag
     1681 tcatagaagc ttcagagggg gacctcatag accgttaacg cgatttgacc ttttgcgcca
     1741 gggcgtttgg atcgcattcc gaatcttttt gggaagtaat cagaggtctc aatgcatccg
     1801 tgtgcgttaa ccaggcctct ttgatcggct caggaacatg gtagagatct agtgtttcct
     1861 ttagaatctg ctgtcgccgg gcgaaatgac cgcccatgat tggatgtttt gcatggactt
     1921 ggtccaacgg acggccttga tattcaaccc cagccccgag gaaatgagcc gttagttgat
     1981 actccatatc tttgatgcgt tgtctatctg cattgcgaaa gaaaaagccg atcatccggt
     2041 catcgaagac tctgtcaata aaagtatcaa tgatcgttct cagcttcggc tcgcctccga
     2101 gctgctcaaa aacagtctgg tccatggggg tgcgtttagg actttgtcga atgctttgcc
     2161 tggaaggaat actgtagtcc gagaatgact acatcattcc gagctggagc cgggcggctt
     2221 cggacatttt gcttggatcc catggaggtt cccacacgag atcaagtttc acatcagtaa
     2281 cgcccgaaac cgctctcacc ttgttttcaa tctccgcagg cagtgactgc actgccgggc
     2341 aatgcggcga tgtgagtgtc attttaatcg aaacgacacc ctcgtcatca acattcactt
     2401 catacaccag tcccaactcg taaatgttaa cgggtatctc agggtcatag caagtacgaa
     2461 gtgcctctaa gacctgctct tccatgacca ggttattgat cgagctcatg atcccatcct
     2521 tttgcggtcg ctcattatga aattcccaat tgcagtttcg cagcccctga gatcatgctt
     2581 tggttccaag gcgggtccca aacgatttcg atatccgcct cccgcacgcc cgcaacgctc
     2641 agcagctttt tctgcatatc tgctttcagg aactgcccca ttccacagcc ttgagccgtc
     2701 aaggtaaacc gcacggtcgc tttatggccg ccaccggata gaggcgtaac ctcgcaatga
     2761 taaatcaaac ccagttcgac gatattcact ggaatttctg gatcgaagca ggatcgcaat
     2821 tgatcccaaa cgcgcttctc cacttctcca tcgttctcaa tcgttgtgga ttcattttgc
     2881 ttaagctctg caacctgcgc cataccaatc gcatcgccat caatgccgtc gatacgtacc
     2941 atgtaaccgc ggtcggtcat cacggtataa gccccgccga gggattgcgt caaccaaacg
     3001 gagctaccgg ccggaagggt ggttgtctcg ccgcttggaa ccatgactgc agggcaatca
     3061 cgcttaacaa caatggactt ttcgctgttc ataaaggcaa ccacctcccg ttcaatttcg
     3121 ctcattcagt ggaggccacc gatttttcgc ctttcagtgc cgagttgacc gtgtgccaag
     3181 cgagactcgc acacttgacg cgggcgggaa acttacatac acccgagaga atcgttagct
     3241 tgccaactcc tctcccgccc tgcggtccat tcttatccgc ttcaccggtg agcatcttat
     3301 gcacattatc gaaaagctct ttggcttcgg tttcggtctt accttttaac tccgcggtca
     3361 ttatcgacgc ggaagccttg gaaatggcgc agcccgatcc ttgaaaactg atatccttga
     3421 taacgccttc ttccaggttg agatagacag tcacatgatc accgcaaagg gggttgtacc
     3481 cctgcgcttt gcggttggca ttcgccatca cccggtaatt gcgcggactc cggttgtggt
     3541 caaggatgac ctcttggtac agctcgtcga gttccgacat tatttaaaca ccttcaacgc
     3601 gcgatgaatt ccccggacca ggacatcgat ttcctcccga gtgttgtaca gagcaaagga
     3661 cgcccgggtg gtcgccggca cgccaaaacg ctgcatgacc ggcatggcgc aatgatggcc
     3721 ggcacgcacc gc
//
LOCUS       DASSWZ010000131         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_329613, whole genome shotgun sequence.
ACCESSION   DASSWZ010000131 DASSWZ010000000
VERSION     DASSWZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329613"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFN58_03265"
     CDS             <1..523
                     /locus_tag="VFN58_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET8917042.1"
                     /translation="GVARTQPVQFSHKHHVSDDGIDCRYCHTSVEESSFAGIPSTKIC
                     MNCHTQIWAESPILAPVRESFRTGKSLEWTRVNNLPGFVYFDHSIHVHKGVGCATCHG
                     RVDQMPLMWRENTLYMEWCLECHRNPERFVRPREQVFSMDWQPPSDQIALGQKLVQQY
                     KIESLTSCSVCHR"
     gene            767..>2250
                     /locus_tag="VFN58_03270"
     CDS             767..>2250
                     /locus_tag="VFN58_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HET8917043.1"
                     /translation="MRPEIWNLKPTSGRLDLASIRERLASSRGQEYWRCLEELSDSEE
                     FHELLKQEFPQQSAGWFEGVSRRDFLRLMGASLALAGLNACRAAPSDEKIVPYVNQPE
                     QIVPGRPLFFATAFPMGGAGAGVLVESHEGRPTKIEGNPNHPASLGATDAFAQASILT
                     MYDPDRSQVVRNAGRISTWNAFLTAINDDLEAERMAGGTGLRILTETITSPTLADQLR
                     QLLAKFPRAKWHQYEPVNRDNVHGGDRLAFGADVNTLYRFENAGVILSLDADFLFSGP
                     ANVRYARDFASKRRVRQGKAEMNRLYVIEATPSITGAMADHRIALRPSEIPNFAGAIA
                     AKMGLSVQVSADAGRSQWIDALVRDLQKHRGTSAVVAGDQQPPAIHALAHWMNQALDN
                     VGKTVIYTDPIEANPVGQLASLRELVKDMESGTVKILLMLDGNPVFTSPVDLGFAEQL
                     AKVPLRVHSALYEDETSAYCHWHIPETHYLETWSDVRAYDGTVT"
BASE COUNT          525 a          589 c          636 g          500 t
ORIGIN      
        1 cggtgtcgca cgcacccaac cggtgcaatt cagtcataag catcacgtga gtgatgacgg
       61 catcgattgc cgctattgcc acacttcggt ggaagagtcc tctttcgccg gcattccatc
      121 aacgaagatc tgcatgaatt gtcacactca gatctgggcg gaaagcccga ttttggcacc
      181 agtgcgtgag agtttccgga ccggcaagtc acttgagtgg acgcgggtga ataatctacc
      241 gggcttcgtc tatttcgatc acagcatcca tgtgcataag ggcgtgggct gtgcgacttg
      301 ccacggtcga gtagatcaaa tgccgcttat gtggcgcgag aacaccctat atatggagtg
      361 gtgtctagaa tgccatcgca accccgagcg ttttgtacgg ccgcgcgagc aggtcttcag
      421 catggattgg cagcccccgt cggatcagat cgcgctcggg caaaagcttg tgcagcagta
      481 caaaatcgaa agtctcacga gctgttcggt ttgtcatagg taagagaggt gtgacgctaa
      541 attctggctg aattggatta gggaaatcgg aaaaaacatg actcgcgcga agacgcaaag
      601 ggcgccaagg ccggagtgga aataagaacg gtttttttaa ggaggtaacg gagataacag
      661 agatgaatta aggaacttcg ttttctctgt tctcctgtta gaagaagttt ttttgtgttc
      721 ttggcgtctt ggcgcgaaaa agaatttatg acttgggata cttaacgtga gacccgaaat
      781 ctggaacttg aaacccacga gtgggcgcct cgatctcgcc tcgatccgcg agcgactggc
      841 atcgtctcgc ggtcaagagt attggcgctg ccttgaggaa ctatccgata gcgaggaatt
      901 tcacgaactc ctaaaacaag aatttccgca acagtcggct ggctggttcg agggagtgag
      961 ccggcgagat ttcttgagac taatgggcgc ctcgctggcg ctggccggtc tcaatgcctg
     1021 cagagcggct ccatcggacg agaagatcgt gccttatgta aatcaaccgg agcaaatcgt
     1081 tcccggccgg ccactgtttt ttgctaccgc gtttccaatg ggcggagctg gtgctggagt
     1141 tctggtcgaa agtcatgaag ggcgcccaac taaaatcgaa ggcaatccca atcatcccgc
     1201 aagtctcggc gcgacggatg cattcgctca ggcgtcaata ctgactatgt acgaccccga
     1261 ccgttcccaa gtcgtcagaa acgccggaag aatcagcacc tggaacgcct tcctcactgc
     1321 gatcaatgat gatctagaag cggagcgcat ggctggtggc acggggctgc ggattttaac
     1381 tgaaacaatc acttcaccaa ctttggcaga tcagttaagg cagttgctgg cgaaatttcc
     1441 ccgcgccaaa tggcatcaat acgaaccggt aaaccgcgac aacgttcacg gcggcgatcg
     1501 tctggctttc ggcgccgatg tcaatacgct ctaccgattt gaaaacgccg gggtgatttt
     1561 atctttggac gctgattttc ttttcagcgg tccggccaac gtccgttatg cgcgcgactt
     1621 cgcgagcaag cgaagagtac gtcaaggcaa ggccgagatg aaccgcctct acgttatcga
     1681 ggcgacgcct tcgatcacgg gcgcaatggc cgaccaccgg atcgcgcttc gtccgagcga
     1741 gattccaaac ttcgctggag ccatcgcggc caaaatgggt ctttccgtgc aagtttcagc
     1801 cgatgctgga cgttctcaat ggatcgatgc gttggtccgt gatctgcaaa agcaccgcgg
     1861 tacgagcgcc gtcgtggccg gtgaccaaca gccgccggcg atccatgcgc tggctcactg
     1921 gatgaaccag gcgctggaca acgttggcaa gacggtgatc tataccgatc ccatcgaggc
     1981 caatccggtc ggtcagctgg cgtcgctgcg cgagctggtc aaagacatgg agtccggcac
     2041 ggtcaaaata ctactcatgc tggacggcaa cccggtattc acatcacccg tggaccttgg
     2101 ctttgcggaa caactagcga aagttccgtt gcgtgttcat tcggctctct atgaagacga
     2161 gacctcggcc tattgtcact ggcatattcc tgagacccat tatctggaaa cttggagcga
     2221 cgttcgcgcc tatgacggta cggtgacaat
//
LOCUS       DASSWZ010000132         5221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_46368, whole genome shotgun sequence.
ACCESSION   DASSWZ010000132 DASSWZ010000000
VERSION     DASSWZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46368"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..483
                     /gene="rpsK"
                     /locus_tag="VFN58_03275"
     CDS             28..483
                     /gene="rpsK"
                     /locus_tag="VFN58_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET8917044.1"
                     /translation="MAKAEDAVEEKPEKKTEGQAEQKPADRAARKKRGRKNISEGVVH
                     IHSTFNNTIVTITDYQGNVISWSSAGSMGFKGSRKGTPFAAQQAADSAAKKAMDHGLR
                     SVQIFVRGPGAGRESALRALQSAGFNISLIKDVTPIPHNGCRPPKRRRV"
     gene            497..1123
                     /gene="rpsD"
                     /locus_tag="VFN58_03280"
     CDS             497..1123
                     /gene="rpsD"
                     /locus_tag="VFN58_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET8917045.1"
                     /translation="MARYTESVCRLCRRENLKLFLKGERCYTDKCAIERRNYPPGQHG
                     QKRPKFSEYSIQLREKQKVKRMYGLLENQFRRTFARASREKGITGEALLVLLERRLDN
                     VAYRLGFASSRAEGRLLVRQGHILVNGKRVNIPSYVVRVGDEVSVKEDSRQMARVLTA
                     MEGAQRRGVPEWAEVNRDAFSGRVKLMPARSDITMPINEKLIVELYSK"
     gene            1154..2173
                     /locus_tag="VFN58_03285"
     CDS             1154..2173
                     /locus_tag="VFN58_03285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET8917046.1"
                     /translation="MYRHWTDLIKPKQLEVDEKSLTSTYGKFYAEPFERGFGQTIGNT
                     LRRILLSSLMGASIVAVRIKGILHEFSTIPGVTEDVTDIILNLKEVRLRLNDGEQQTL
                     KIDAKGPGTVHAKDIVAPANVEILNPEHKIATLSRDAKLEAEITAKLGRGYVPAERNK
                     EEGAPVDTIFIDAVFSPIHKVNFTVTNARVGQRTDYDRLVFEVHTDGSVKPEDAVAYA
                     AKILQDQVGIFVNFQDEPRQEKREDGPTIPLNDNLFRSVDELEFSVRSQNCLQNADIK
                     YIGELVQKTEQEMLKTKNFGHKSLNEIKEILREMGLELGMKIDHFPSREEIENRRRAS
                     EKETA"
     gene            2177..2638
                     /gene="rplQ"
                     /locus_tag="VFN58_03290"
     CDS             2177..2638
                     /gene="rplQ"
                     /locus_tag="VFN58_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET8917047.1"
                     /translation="MRHLKSGRKLSRTPSHRWALMRNLITCLLRDERIQTTDPKAKEV
                     RRWADRVITLGKRGSLHARRQALEIVRDKAVVRKLFDTLAPRFKDRPGGYTRIIKIGW
                     RHGDSALISVIELVASAEDAVEAPSGRRRRRSKGRASAQEAGAQGKNARTA"
     gene            2801..3193
                     /locus_tag="VFN58_03295"
     CDS             2801..3193
                     /locus_tag="VFN58_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8917048.1"
                     /translation="MRHEGKGKPMGLLHRSLNDILRIDLNVVALDELQWRDFGKGLSM
                     SRLAREGKRELILYRIAANAASDAFLKHEHIGGEFYLVLRGKIADETGEYVEGDLVFL
                     DPQSVHTPRAVGDTVVLVLWPEGVRIVD"
     gene            3222..4106
                     /locus_tag="VFN58_03300"
     CDS             3222..4106
                     /locus_tag="VFN58_03300"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8917049.1"
                     /translation="MKTLGFIGTGGMGSGMASNLIKAGYSLVANDLRREQVRGLEGQG
                     ATFKESPRAVAESCEMVLSMLPYNEAVRAVGLGGDGLHEATSGAKLWVDFSSIDKKTI
                     VGVNAELQKKGWTILDGSAGGVEEAAAAGTLSLWISGSKELFDQHQPVFKAMGKKVLH
                     VGDLGNAKLVKNAMAMFAAIQHLGLAEIFSWLKKGGVTEEAFYTVLQNSQQSSEAINR
                     ICEIVVSRKYKPRKSWMPKDVGFGLDMAREMEVPVPFTALAYQMFSIAQATGLDGYEA
                     TGIACNVFDLITGQKKGY"
     gene            4117..4656
                     /locus_tag="VFN58_03305"
     CDS             4117..4656
                     /locus_tag="VFN58_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HET8917050.1"
                     /translation="MFPPERISRFLTYLLRHRPAEYPIAFDRHGFAPWAEVVDTVRGR
                     FREVTEEQMCAVVIGSDKQRFELKDGKIRATYGHSFPVDLGLEPVQPPPQLYYGTARD
                     LAQSILRTGLKPRDRQYVHLSSSVNEAIAVGKRRDPTPAIIVVDAVAAHAEGVRFFAS
                     GPLFLTENISAKFLSLDQK"
     gene            4848..>5221
                     /locus_tag="VFN58_03310"
     CDS             4848..>5221
                     /locus_tag="VFN58_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET8917051.1"
                     /translation="MYLRTLLILIVLGAVVIFAAINWNAFMAPTTLSVVVATVEAPLG
                     LILLVVVGLLTLLFLLYVVYLQSSILVENRRNARELQAQRELADQAEASRFSQLRSFL
                     ERELGRLGEKTEESKVGVLAKL"
BASE COUNT         1263 a         1294 c         1542 g         1122 t
ORIGIN      
        1 aagggttagt cccaatagga gaaccacatg gcaaaagccg aagatgccgt cgaggaaaaa
       61 ccggaaaaga aaactgaagg gcaagccgag cagaaacctg cggacagggc cgcgcgcaag
      121 aagcggggac gaaagaatat ctccgagggc gtggttcata ttcattcgac cttcaacaac
      181 acgatcgtta cgattacgga ttaccaaggt aacgttatct cgtggtcaag tgccggaagc
      241 atgggtttca aaggctcgcg caaagggacc ccgtttgcgg cccagcaggc cgcggatagc
      301 gcggccaaaa aggcgatgga tcatggactt cgaagtgttc agatctttgt ccgaggtcct
      361 ggtgcggggc gagaatcggc gctgcgagcg cttcagtcag cgggtttcaa catcagcttg
      421 attaaagatg taaccccaat tccccacaat gggtgccgcc cgccgaaacg aaggcgggtc
      481 tgataaggag gaaggattgg ctagatacac agaatctgtg tgccggcttt gcaggcgaga
      541 aaacctcaag ctctttctga aaggggagcg gtgttacacg gacaagtgtg ccattgaaag
      601 gcgcaactat cccccggggc agcatggtca aaaaagaccc aaattctctg aatacagcat
      661 tcagctgcgt gagaagcaaa aggtcaaacg gatgtacggg ctgctggaga atcaatttcg
      721 tcgtaccttc gcgcgggcga gccgcgagaa ggggattacc ggagaggcct tgctagtttt
      781 actggagcgg cggctggaca acgtcgccta tcggcttggg tttgccagtt cccgagcgga
      841 aggccgattg ctcgttcggc aggggcatat tctggttaac gggaaacgag tcaacattcc
      901 gtcttatgtt gttcgtgttg gcgacgaagt ttccgttaaa gaggatagtc ggcagatggc
      961 gagagtcttg acggcgatgg agggcgcaca gcgtcgcggt gtgccggaat gggcggaagt
     1021 caaccgggat gcatttagtg ggcgggtcaa actaatgccc gcacgcagcg acatcacgat
     1081 gcccatcaac gaaaaattaa ttgtagaatt atattcgaag taattttcga atgtgggaga
     1141 cgctatgtcc acaatgtatc ggcattggac ggatttgatc aaacctaagc aactggaagt
     1201 cgatgagaag tcattaacct ccacctatgg caagttttat gcggagccct ttgagcgcgg
     1261 ttttggccag accatcggta acaccctgcg gagaatcctg ctttcctcgc tgatgggcgc
     1321 atccattgtc gccgttcgga tcaagggaat tctccatgag ttctcaacca tccccggggt
     1381 tacggaagac gtcaccgata tcatattaaa tctgaaagag gtgcgactcc ggctcaatga
     1441 tggcgagcag cagacgctta aaatcgacgc caaagggcct ggaacggtgc acgcaaaaga
     1501 tatcgtcgct cccgctaacg tggaaatctt gaatcctgaa cataaaatag ccaccctatc
     1561 gcgggacgcc aaattagaag cagagatcac tgcaaaactc ggtcgcggtt atgtccctgc
     1621 agaaaggaac aaagaagaag gggcaccggt ggatacgatt ttcatcgacg cggttttttc
     1681 tcccatccac aaggtgaact ttacggtgac caacgctcgc gtgggccagc ggaccgatta
     1741 tgatcgtttg gtgtttgagg ttcacaccga tggtagcgtc aagccggaag acgccgtggc
     1801 ctatgcagcg aagattctcc aggaccaggt tgggatcttc gtgaactttc aggacgagcc
     1861 ccgtcaggaa aaacgtgaag acggacccac gattccattg aacgataatc tcttccgcag
     1921 cgtcgatgag ttggaatttt ccgtgcgctc gcagaactgc cttcaaaacg ctgatatcaa
     1981 gtacattggt gagctggttc agaagaccga acaagagatg ctgaagacga agaatttcgg
     2041 ccacaagtcg ctcaacgaga tcaaagaaat tcttcgtgag atggggcttg agcttggcat
     2101 gaagatcgat catttccctt cccgagaaga gatcgaaaac cgccgccgag ccagtgagaa
     2161 ggaaacggca taaactatgc gccacctcaa gtcgggcaga aagctgagtc gaactcccag
     2221 tcaccggtgg gctttaatgc gcaatctcat cacctgtttg ctacgcgatg agagaattca
     2281 aacgacagac ccgaaagcca aagaggtgag acggtgggct gatcgggtta ttacgttggg
     2341 caaacgaggt agcttgcatg ccagacgtca ggccctggaa atcgttcgtg acaaagccgt
     2401 cgtgcgcaag ctgttcgaca ctctcgcgcc acgcttcaag gatcgtcctg gcggctatac
     2461 ccggatcatc aaaatcggtt ggcggcatgg agattcagcg ctgatttcgg tgatagaact
     2521 agtggccagc gcagaagacg cagtcgaagc tccgtccggg agaaggagaa gacgaagtaa
     2581 aggaagggca tcagcccaag aggccggcgc ccaaggcaaa aacgcgcgga ccgcgtagta
     2641 tcgtcatccc ttttgtcggc caaacaagag ccggagccgg gtggtttatt ttacctagcc
     2701 tccggctttt ttattgcccg ggtgcgtatc gagcaccagc gtgaaaatcc tcgcgccagt
     2761 atatggaacc gatagctttt gttcgccgga cggtcgtgat atgaggcacg agggaaaagg
     2821 aaagccgatg gggttactac atcgatcgct caacgatatc ttgcgcattg acttaaacgt
     2881 cgttgctttg gatgagctgc agtggcgcga cttcggcaag ggtctctcca tgtcccgact
     2941 agcgcgggaa gggaaacggg agttgattct ctatcgaatt gccgccaacg ccgcttcaga
     3001 cgcgtttctg aaacatgagc acatcggcgg tgagttttac ttggtcctta ggggcaagat
     3061 cgccgatgaa actggggagt atgtggaagg cgacctggtt tttttggatc cgcagtcggt
     3121 ccatacgcca cgggcagttg gcgatacggt agtattagtg ctatggcccg aaggagtgcg
     3181 catcgtagat tgaacaggac caatcaacga cgggaggatt tatgaaaacc ttgggattta
     3241 tcggtacggg cgggatgggc agtggcatgg cgtcaaactt gatcaaagcg ggttacagct
     3301 tggtcgctaa tgatttgcgg cgggagcagg tgcggggatt ggagggtcag ggggcaacgt
     3361 tcaaggagtc gccccgagcg gttgccgagt cctgtgagat ggtgctttcg atgctgccgt
     3421 acaatgaagc agttcgagcg gtcgggctgg gcggtgatgg attgcacgaa gccaccagcg
     3481 gcgccaagct gtgggtcgat ttcagcagta tcgacaagaa gacgattgtt ggagtcaatg
     3541 ctgagctcca gaaaaaagga tggactattc tcgacggctc tgccggcggt gtcgaagaag
     3601 ccgcagcggc gggaacgcta tcgctgtgga tttcgggatc gaaagaactg tttgaccaac
     3661 atcagcccgt gtttaaagcg atgggcaaaa aggtcctcca tgtcggcgat ctgggcaacg
     3721 ccaagctggt gaaaaacgct atggcgatgt ttgctgcaat tcaacacttg gggctcgcag
     3781 agattttctc ctggctcaag aagggcggcg tgacggaaga ggccttttac accgtcttgc
     3841 agaattccca gcagagctcc gaagccatca accgcatctg cgagatcgtt gtgagccgga
     3901 aatacaaacc ccgcaagtcg tggatgccca aagatgtcgg cttcgggcta gacatggctc
     3961 gcgagatgga agttccggtt cctttcactg ccctggcata tcagatgttt tctatcgccc
     4021 aggccaccgg tcttgacgga tatgaggcta ccggcattgc ctgcaacgtg ttcgatctga
     4081 ttacagggca aaaaaaggga tactgaagga gggggggtgt ttcctccgga acgcatctcg
     4141 cgctttctta cctatctctt acgccatcgg ccagcagagt atccgatcgc gtttgaccgc
     4201 cacgggtttg ccccgtgggc cgaggttgtc gacacggtgc gggggcggtt tcgagaagtc
     4261 actgaggagc aaatgtgcgc cgtggtcatc ggttccgaca agcaacgctt cgaattaaaa
     4321 gatggaaaaa tccgcgctac ctacggtcat tcttttccgg tggatctcgg cctcgaaccc
     4381 gtgcagccgc caccgcaact ctactatgga accgcgcggg atctcgctca atcgatcctg
     4441 cgcacagggc ttaagccacg tgatcgccag tacgttcatc tcagttcatc ggtgaatgaa
     4501 gccatcgcgg taggaaagcg acgggatcct acccccgcga tcatcgtagt ggatgccgtg
     4561 gccgcgcacg ccgaaggcgt tcgcttcttc gcctccggcc ctttgtttct tacagagaac
     4621 atttccgcta aattcctgtc gttggatcag aagtgaagcg gctccgttca agcgatttgg
     4681 aggcgcagca aagcgtcagc gtctagttga atctatgcca aaccgttggc gccatgtttc
     4741 ctttcgcttc ggttacgcag aacacgatcg ggcaactaac tcgtaaagcc ttgcatctgg
     4801 cggactcgag acggcgaacg aaacttgtcc aatcaaggga ggtctgtatg tacttgcgca
     4861 cattactcat cctgattgta cttggagccg tggttatttt cgcggccatc aactggaatg
     4921 cgttcatggc accgacaacg ttgtcagtgg ttgtcgccac cgtggaggcg ccgctcgggc
     4981 ttattttgct cgtcgtcgtc ggtctgctca ctttgttatt tctgctgtac gtggtctatt
     5041 tgcagtcctc catcctcgtg gaaaaccggc gcaatgcgcg cgaactgcaa gcgcagcggg
     5101 aactcgccga ccaagcggaa gcttcccggt tcagccaact ccgctcattt ctggaaaggg
     5161 agctgggacg gctcggggag aaaaccgagg aatccaaggt gggtgtgtta gcgaagcttg
     5221 a
//
LOCUS       DASSWZ010000133         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1221153, whole genome shotgun sequence.
ACCESSION   DASSWZ010000133 DASSWZ010000000
VERSION     DASSWZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221153"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFN58_03315"
     CDS             <1..185
                     /locus_tag="VFN58_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HET8917052.1"
                     /translation="TEKYIDVSQWFKGPVFEPLKDPKFFKKFFIEAGTLAWPNGVDIA
                     PEALYTAADSKKTHNQ"
     gene            complement(517..1608)
                     /locus_tag="VFN58_03320"
     CDS             complement(517..1608)
                     /locus_tag="VFN58_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8917053.1"
                     /translation="MTSINEIFRTFGPEYLQRYANSMPKTHLKVIDAMIACRTEACGL
                     ALYQCASCARSHELYRSCGNRHCPTCQQHKTRQWLEKQINRQLPTHHFLFTFTVPENL
                     RPLMRKNQRAAYSALFKTSSEAIKKLAPDQHYLGGNLPGFFGVLHTWGRTLQYHPHIH
                     YIVPGGALSSTDGRWHPSTTDFYLPVKALSKIFRAKFRDEMKKAGLFDQIPSEIWQSD
                     WNVDSQAVPSSEACLKYLAPYVFKVAISNSRILKVQDRTVLIRYRKPHSSRSRILPLE
                     VMEFIRRFLQHVLPTGFMKVRYYGFMNPNCGVALDHIRGLIELTIGFQIDLPKAALQP
                     SRPIICPNCGGRLKLRFLLSPAQVLLWSG"
     gene            complement(1592..2482)
                     /locus_tag="VFN58_03325"
     CDS             complement(1592..2482)
                     /locus_tag="VFN58_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET8917054.1"
                     /translation="MMNDWYQSMVDNLQLNGKGERTQQAYTRAVRMLSQFYGKTPDLV
                     SEQELQEYFLQRKNVNHWSPKTMRICYCGIRFFYEKVLDRNWRILGILRAQNERRLPA
                     VLSVDEVRSLLSHIKTFHNYAYLSTVYSCGLRLHEGLHLEVSDIDSQRMMIHVHRGKG
                     AKDRYVPLPQSTLQLLRRYWLTHRHPGLVFPALGRSGQGATQAQTPMAKSSVQGAFRR
                     AKFDAGIAKKGVAIHTLRHSYATHLLEAGVNLRVIQRYMGHAQLETTMLYLHLTQKGQ
                     EDACQLINHVMEGLDHDLHQ"
     gene            complement(2672..>2959)
                     /locus_tag="VFN58_03330"
     CDS             complement(2672..>2959)
                     /locus_tag="VFN58_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8917055.1"
                     /translation="NKLDAIVHKAVEHQPTFIKDGVNPPFVDQKGAPHINTFLVFVPS
                     IVVPASFTRDNLPAGITFLGRPYDDGNMIKFAYAYEQATHHRRPPASTTPL"
BASE COUNT          678 a          624 c          955 g          702 t
ORIGIN      
        1 gaactgagaa gtacatcgat gtttcgcagt ggttcaaagg cccagtgttt gagcctctga
       61 aggacccaaa gtttttcaaa aagtttttca tcgaagcggg aacactcgcg tggccaaatg
      121 gcgtcgatat agctcctgaa gccttgtaca ccgcagctga ttcaaagaaa acgcataacc
      181 aataaggatt gagacgactt cagaagtcgt ttcaatccat tgttcaagaa gcccgtcatc
      241 gtggcagcgg ctatcaatat aaagggtatt ggttgtagag gttggttggc gccgagggtt
      301 ttggccatca ggctcgcagt gcgagagggc aagaagcgaa tttgcgatgc cggttattat
      361 tcaagctcag cgattttttc agccagaggc cccgcagttg agggcgtgta gagtgaattt
      421 gttcgtcata cgggcgcact tcaccccatc cctttgcgca gcggtttagt tcaacacagg
      481 gaaagcctca gggatttagt tttttgctgc caccttctat cctgaccaca aaagaacctg
      541 ggcgggggag agtaagaaac gcagtttaag gcgaccgcca cagtttggac agatgatggg
      601 ccggctgggt tggagagcag ctttgggtaa gtcgatttgg aatccgatgg tcaattcgat
      661 caggcccctg atgtgatcga gagcgacccc acagttggga ttcataaatc cgtagtaacg
      721 gactttcatg aagccggtag gcagcacgtg ttgtaagaag cgccggatga actccatgac
      781 ctcgagggga aggattcttg agcgggagct gtgcggtttg cggtagcgaa tgaggacggt
      841 gcggtcttgg actttcaaga tacgtgagtt ggagatggcg actttgaaaa cgtaaggggc
      901 gaggtatttg aggcacgctt cgctggaagg cacggcttgg gagtcgacgt tccagtctga
      961 ctgccagatt tcagagggga tttgatcgaa cagacccgct tttttcattt cgtctcggaa
     1021 cttggctcga aagattttag agagcgcttt gacggggaga tagaagtcgg tagtggaggg
     1081 atgccagcgg ccatcggttg aggacagcgc gccgccgggg acgatgtaat ggatatgagg
     1141 atggtactgt aaggttcgtc cccaagtgtg aagcactcca aagaagcccg gcagatttcc
     1201 accgaggtaa tgttgatcgg gggcaagttt tttgatggcc tcagaagagg tcttaaacag
     1261 ggcagagtaa gcggcgcgct ggtttttgcg catcaagggg cgcaagttct caggcaccgt
     1321 gaaggtgaaa aggaaatggt gagtggggag ctggcggttg atttgttttt ctaaccactg
     1381 tcgggtctta tgctgttggc aagtcgggca gtggcgattg ccacaggagc gatagagttc
     1441 atgggaccga gcgcaggatg cgcattggta gagcgcaagg ccgcaggctt cggtgcggca
     1501 ggcaatcatc gcatcgataa ctttgagatg tgttttaggc atgctgttgg cgtagcgttg
     1561 gaggtactca ggaccgaagg tgcgaaagat ctcattgatg gaggtcatgg tcgagtccct
     1621 ccatcacgtg attgatcaac tgacaggcat cctcctggcc cttttgggtg aggtggagat
     1681 agagcatggt ggtttcgagt tgggcatggc ccatgtagcg ttggatgacg cgcagattga
     1741 cgccggcttc gaggaggtgg gtggcgtagg aatggcgcag cgtgtgtatg gcaactccct
     1801 ttttagcgat accggcatca aacttggcgc gacggaaggc accttggaca ctgcttttgg
     1861 ccatgggagt ttgagcttgc gttgccccct ggccgcttcg gccgagggca gggaagacaa
     1921 gaccgggatg gcggtgggtg agccagtagc ggcgcaagag ttggagggtt gattggggca
     1981 gagggacgta gcgatccttg gcgcccttac cacggtggac atggatcatc atgcgctgac
     2041 tatcgatatc ggagacttcg agatggagac cttcgtggag tctgaggccg caggagtaga
     2101 cggtggagag ataggcatag ttgtggaagg ttttgatgtg ggaaagaagg cttcggactt
     2161 catcgacgct taagacggcg ggcaggcgtc gttcattttg ggctctaagg attccaagga
     2221 tgcgccaatt gcggtcgagg accttctcgt agaagaaacg aattccgcaa taacagatac
     2281 gcatggtttt gggggaccag tgattgacgt ttttgcgttg caggaaatac tcttggagtt
     2341 cctgctccga aaccagatcg ggggttttgc cgtagaactg cgaaagcatg cgcaccgcgc
     2401 gggtgtaggc ctgctgcgtg cgttccccct tcccattgag ttgtaaattg tcgaccatac
     2461 tctgatacca atcgttcatc atgacagctc tcctttcgtt aaatagtttg aatccaaact
     2521 caggagagct atttatctaa gaacgatggc gatgggatag ggagaatgtc aggtaggatt
     2581 aagatctgcc gcgcagcggc ttacttgaac ataggcatgg agccgacgcg ctgaagttag
     2641 tcgtgaatct gccaatttcg agaaaagttg ctcacaatgg cgttgtgctc gcgggtgggc
     2701 ggcgatgatg cgtcgcctgt tcgtaggcgt aggcgaattt gatcatattg ccatcgtcgt
     2761 aggggcgtcc gagaaaagtt atgccggcgg gcagattatc gcgggtgaaa ctcgcgggca
     2821 cgacaatgga agggacgaag acgagaaacg tgttgatatg gggcgcgcct ttttggtcaa
     2881 cgaaaggcgg attgactcca tctttgatga aggtcggctg gtgctcgacg gccttatgca
     2941 cgatagcgtc cagcttgtt
//
LOCUS       DASSWZ010000134         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1093664, whole genome shotgun sequence.
ACCESSION   DASSWZ010000134 DASSWZ010000000
VERSION     DASSWZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093664"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFN58_03335"
     CDS             <1..499
                     /locus_tag="VFN58_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET8917056.1"
                     /translation="THRTVLIENGILNGYLQDRLNASLMKMPLTGNGRRESYAHIPMP
                     RMTNTFMLAGQSEPEDVLRSVRKGLYAVSFGGGQVDITNGKFVFSASEAYLIEDGKVT
                     KPVKGATLIGNGPDVLTRVSMVGSDLKLDEGVGTCGKDGQSVPVGVGLPTIKIDGLTV
                     GGTRA"
     gene            593..1948
                     /locus_tag="VFN58_03340"
     CDS             593..1948
                     /locus_tag="VFN58_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET8917057.1"
                     /translation="MERLELSQELGRDILAMAKALGASQGDVVMVESDSFFVTVRLGE
                     VEKISQAQEKRLGLRLFLGSSSASASTSDISKKSVARLVEDTTRMARATAQDPHSGLP
                     AAEALAREIPELDLLDADGRSISVEEKIQMALAAEKTALEYDPRITNCEGGEFSNQFG
                     RVIYVSSHGFSGEYEGSTFGHSVAPVAAQNGAMQRDYWYSSNRKFSRLESPKKVGEKA
                     AQRVLRRLGAKKVKTCEVPIVFDPEIAASLLRNLSSAISGYSLYKGASFLRDKLGAQI
                     ASDIVTVIDDGTIPAALGSRPFDAEGLPTTRKIIVEKGRLQSYLLDTYSGRKLGMPST
                     GNASRSVGEPPGVAPSNFYLTPGTHSADEIIRSVKQGFYVTELIGFGVNMVTGDYSRG
                     ATGLWIENGELSYPVEEVTIAGNLKEMFQNIEMVGADLEMRGRIAAPTIKISQMTVAG
                     N"
BASE COUNT          517 a          542 c          643 g          499 t
ORIGIN      
        1 gacacaccgg acggttctca tcgaaaatgg tattctcaac ggctatctcc aagatcgtct
       61 caacgcgtcg ctcatgaaga tgcccttaac tggcaacggg cggcgtgaga gttacgccca
      121 cattccgatg ccgcgtatga caaatacctt catgttggcg gggcaaagcg aaccggaaga
      181 tgttcttcgc tcggtaagga aaggactcta cgcggtttca ttcggcggcg gtcaggtcga
      241 tatcaccaac ggaaaatttg ttttctccgc cagtgaggcc tatttaatcg aggacggtaa
      301 agtcaccaag ccggtcaagg gagctacgtt gatcggcaac ggaccggatg ttctgactcg
      361 ggtttctatg gtgggctcgg atttgaagct agatgagggt gtcggaacgt gcggtaaaga
      421 cggccaatcc gtgcccgttg gagtagggct tccgaccatc aaaattgatg gcctaacggt
      481 gggtggcacg agggcatgac atcggttcaa aaggttcaag ggacgttcga actgcgtgac
      541 atctcttgcc cgttgattgg cttgaacggt ttgcactact tgtagggaga ttatggaacg
      601 gctggaactc agtcaggaac ttgggcggga catactcgcc atggcaaagg ctctgggcgc
      661 ttcccagggc gatgtcgtta tggttgagag cgactcgttt tttgttacgg tacgtctggg
      721 ggaagtggag aaaatcagtc aggctcagga aaaacgcctg gggcttcgct tgtttctcgg
      781 ctctagctcg gcttcggcgt caacctccga tatctcaaag aaatcagttg cccgtttggt
      841 cgaagatact acgcgaatgg ctagggccac ggcgcaagat ccgcacagcg ggctgccggc
      901 agccgaagct cttgctcgcg agattcccga gctggaccta ttggacgcgg atggtcgatc
      961 catctcggtg gaagaaaaaa tccaaatggc gctggcggcg gaaaaaactg ccttagagta
     1021 tgatccccgg attaccaact gcgaaggtgg ggagttctcc aatcagttcg ggcgggtcat
     1081 ctatgtcagc agtcacggtt tttccggtga gtacgagggt tcaaccttcg gtcactctgt
     1141 ggcgccggtt gccgcacaga acggtgccat gcaacgggac tattggtatt catcgaatcg
     1201 gaaattctcg cggctggaat ccccaaaaaa agtcggcgag aaagcggcgc agcgggtgtt
     1261 gaggcggctc ggggctaaaa aggttaaaac ctgcgaagtt cccattgtct ttgatcccga
     1321 gatcgccgcc tctctgctac ggaatttatc atccgctatt tccggctatt cgttgtacaa
     1381 gggtgcatcg tttctaaggg ataaacttgg agcccaaatc gcttcggata tagtcacggt
     1441 gattgacgat ggaaccattc cggccgcgtt gggctccaga cccttcgatg ccgagggctt
     1501 gccaacgacg aggaagatca ttgtagaaaa aggacgcctc cagagttatc tcctggacac
     1561 ctacagcggt agaaaattgg ggatgccgtc caccggcaat gcttcacgct cagtgggaga
     1621 accgccgggt gtggcaccgt caaatttcta tttgactcca gggacacact ctgcggacga
     1681 aattatccgc tcggtgaagc aaggtttcta cgtcacagag ctgatcggct tcggggttaa
     1741 tatggtgacc ggcgactatt cccgcggcgc aaccggcctg tggattgaaa acggcgagct
     1801 gagttatccg gtggaagagg tgacgatcgc cggaaatctc aaagagatgt ttcaaaatat
     1861 cgaaatggtg ggagctgatc ttgagatgcg cggcagaatc gccgcgccca ccatcaagat
     1921 ttctcaaatg accgtggcgg gaaactgagt tttggttcaa agttccgggt tcaaagttca
     1981 aagtgtaaat cccgggtggc cgatcgtttc atggaacgtt gaacattcag agtaccttaa
     2041 agcctgcctt gagtaccgca cgcaaaaagt catcgatgtg agactgagtc ggatcgaaat
     2101 cgaccaccgc gacgctccgt tctagattga aatcgacagt atccaccccc ggaacgctct
     2161 gcagcgcttg cttgagcacc tggagatctt cggccttcag g
//
LOCUS       DASSWZ010000135         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1051320, whole genome shotgun sequence.
ACCESSION   DASSWZ010000135 DASSWZ010000000
VERSION     DASSWZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051320"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFN58_03345"
     CDS             complement(<1..462)
                     /locus_tag="VFN58_03345"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917058.1"
                     /translation="MGRTWIKYRLVLTVAVFLVVIPWVRAEGQEKIRIAYSSTDTLNS
                     LWTVADDAGFYKKHGLDAEVVYIGSTTVGVAAIVSQDVQVGNAAGSGVANAVVRGADT
                     VSVACFINVLAYELVVLDSVKSAEDLKGKSIGISRFGSVSDVAARELIRGLG"
     gene            734..1582
                     /locus_tag="VFN58_03350"
     CDS             734..1582
                     /locus_tag="VFN58_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917059.1"
                     /translation="MSAKIRHLALYTENQDRMATFYQQVFGMKRITRSFNDPNKGHIS
                     DGVIGLAVLNRRPGFAAALDHFGFEVDDVGDILDVMKQKYPKTLVTKGLEQVAFAVFR
                     SHDPAGTQFDISWKEHPKVQQGYKEDGWQQPRQINHIALRASEPEQVAEFYHEVFGLK
                     EGKNFRDEDTLCVTDGTVDLVIRRTTNHSYTSMRQGLDHFGFAVESLEDAKKDLAELA
                     TSFPASVPKDIAGGIFGHITKKDMDGCRIGQYSLSDPDGIVLDLSERAASQGTRSSEQ
                     SQPSTG"
     gene            1627..2589
                     /locus_tag="VFN58_03355"
     CDS             1627..2589
                     /locus_tag="VFN58_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917060.1"
                     /translation="MNHARRISLSLVITFLCGALYVANAETLTPVHIGVPTNSATWFP
                     LYVAWKKGIFRDLGLELLPITMQARTALAALASKQIGFITQIGSPMTAISRGLPASLI
                     MVLCERSHHVLVTKPEITSPAQLWGRRIAISQPGGTVHRELLMILEKFRIDPNEVKMV
                     NLASTPNGVTALKGGNVDAAMLLIPHDLYLEKDGFRPLVYLKDIAEFPLAGLVVHNDR
                     LRDRPDEVKKVLVGAIRGIAYTKTRREEVLPLLKEFLGLENLAMAKTAYERLRDIWSD
                     SGMVSEKGLRAAATLAEVPSSFPLDKLANWSFVNEAASSVRTNH"
BASE COUNT          716 a          749 c          756 g          649 t
ORIGIN      
        1 gcccagtccc ctgatcaact cgcgcgccgc cacgtcggac acgctgccga agcggctgat
       61 gccgatggac tttcctttta ggtcttcggc ggatttgacg ctgtcgagca cgaccagttc
      121 gtaggcaaga acgttgatga agcaagctac gcttaccgtg tccgcgccgc gcaccaccgc
      181 attggccacg ccgcttccgg cagcattacc aacctgaacg tcctgagaga caatggcggc
      241 gacgcccacc gtggtgctcc ctatgtagac gacttccgcg tccaagccat gctttttgta
      301 aaagcccgca tcgtcggcga cggtccaaag cgagttgagc gtatctgtgg agctgtaggc
      361 aatccgaatc ttttcctggc cctcggctct cacccacggg atcacaacta agaaaactgc
      421 gacggtcaaa accagacgat actttatcca agttctgccc atagaacccc ctttcactgg
      481 gccatttaac tagacctttc ttctaatttc gtcaatgacc ttaactcatc aaaacatgca
      541 agggatgttt acccgacatt gactacagta gttctccgta agggtggggg caaaaagtct
      601 acaatgacgt ctcagaccag caaagtgttt gacgcagtat cttcactttt ggtaagagcg
      661 cagattaggg ctggaataaa tcgcgcaacc gtcgcttgtg aagaagggtt ggctctcaag
      721 aaaaggagga atcatgtcgg ctaagattag acatctcgct ctctatactg agaatcaaga
      781 tcggatggca actttttatc aacaggtatt cgggatgaaa aggatcacgc ggagttttaa
      841 cgatcctaac aagggccaca tcagcgatgg agtcataggc ctcgccgtgc tgaaccggcg
      901 tccgggcttt gcggccgcgc ttgatcactt cggcttcgag gtggacgacg ttggcgatat
      961 cctggacgta atgaagcaga agtatccgaa gactctggtc accaaaggat tggagcaagt
     1021 ggcattcgcc gtattcagaa gtcacgaccc ggctggaacg caattcgaca tttcgtggaa
     1081 agagcatccg aaagttcagc aaggttacaa ggaagatggt tggcagcaac cacggcagat
     1141 caatcatatc gccctccggg cttcggaacc cgagcaggtg gccgaattct accatgaggt
     1201 tttcggtctc aaggaaggga aaaattttcg cgatgaagat acactctgcg taaccgacgg
     1261 cactgtagat cttgtgataa gacggacaac gaatcattcc tatacgagca tgaggcaggg
     1321 gctcgatcac ttcggcttcg cggtggagag cttggaagac gctaaaaaag accttgctga
     1381 acttgcaacc tctttccccg cgtctgttcc gaaagacatt gccggtggga tcttcgggca
     1441 cataacaaaa aaagatatgg acggctgtcg cattggccaa tattcgctct ccgatcccga
     1501 cggtatagtc ttggatcttt ccgaacgagc ggcatcacaa ggaacgcgtt cgtccgagca
     1561 gtcgcaaccc tcgacaggtt aaaggagagg agccgagtcg atgcgctaca gtgaacggcg
     1621 gataccgtga atcacgccag acgtatcagt ctgtcgctag tcattacctt tctatgcggc
     1681 gctttgtatg tcgccaatgc tgagacattg acccccgtac atatcggcgt tccaacgaat
     1741 agcgcgactt ggtttcccct ttatgtagcc tggaagaagg gaatattccg cgatctaggg
     1801 ctcgaactgc tgccgattac catgcaagcc cgcacggctt tggccgccct cgcgtcaaag
     1861 cagattggat ttataaccca aattggctct ccgatgacgg ctatttcacg cggcctgccg
     1921 gccagcctca tcatggtgct ttgtgaaagg tcccaccacg ttttggtgac aaagcccgag
     1981 attacttcgc cggctcaact atggggccga aggatagcga tcagccaacc gggcggcacc
     2041 gttcatcgcg agttgcttat gatcctggag aagttcagga tcgatccgaa cgaagtaaaa
     2101 atggtcaact tagcaagcac gccaaacggc gtgaccgccc tcaagggcgg caatgttgat
     2161 gcggcgatgt tgttgatccc tcatgacctg tatctagaga aagatggatt tagaccgctg
     2221 gtctatctta aggatatcgc ggaattccct ctcgccggtc ttgtcgtaca taacgaccgg
     2281 cttcgcgacc ggcctgacga agtgaaaaaa gttttggtcg gtgcaataag gggaatcgct
     2341 tatacaaaaa cgcgcaggga agaagtgctc cctttattga aggaattcct tggccttgag
     2401 aatttggcaa tggccaaaac agcgtatgag agattgagag atatttggtc tgacagcggg
     2461 atggtttccg agaaagggtt gagagccgcg gcaaccttgg cggaagtacc aagttctttc
     2521 cctctcgaca aactggccaa ttggtcgttc gtaaacgaag cagcatcatc ggttagaacc
     2581 aaccactaac gcgaattgct ttggcggatc gcccttcagc ggcagtggcc agcttctggc
     2641 agtacgcgcc agctccggcc caaaccgcca agacttgcac cgctacccgg caacgaaaac
     2701 cctcatcggt ggcttctcct cacaatggct aaggtttttc ttttgttata ttgtcgccgg
     2761 ttgcattata attagcgtta gcgctagcga gtcctcgatt accacggctc gatggtaagc
     2821 gcccagtaga aaaaggacgt ggctggaatc aaaatagtct aaatctgagg
//
LOCUS       DASSWZ010000136         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1306113, whole genome shotgun sequence.
ACCESSION   DASSWZ010000136 DASSWZ010000000
VERSION     DASSWZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306113"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1684)
                     /locus_tag="VFN58_03360"
     CDS             complement(203..1684)
                     /locus_tag="VFN58_03360"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET8917061.1"
                     /translation="MLRDDLAREISAVLGAESGVHLTKPERRENGDWSTNAPFLLAKK
                     LSRKPAEIAADAAAKLSGSSKMIERAEAKGGFLNIFVKDEVLLKTLSAKTEFPVKKEK
                     INIEFVSANPTGPLTMANGRGGFYGDALATILRKVGYNVTREFYINDSGNQVRLLGES
                     IEAAEGKREAKEEYYKGEYVKTLAGKNAEEAVQTLLLEIKLSLKKAGIEFDTWFSEQK
                     LREQGELEKVLKFLESKGLLDKKDDAIWLGDAVLIKSDGEPTYFLSDLAYHYDKFLKR
                     QFDIAIDVWGADHHGYVDRMKKGIAALGINPERLKIIIMQLVRLMQGGTEVRMSKRAG
                     EFVTMDELLEEVGVDAARWFFLERGTNTHMDFDLDLAKEKSEKNPVYYVQYAHTRMAS
                     ILQKSTGRASPLKVFKNEAERELALKILRYPEILDDISRDYGVQRLTSYAYELAQAFS
                     AFYRDVKVIGSDREAELLYLVLKTKETLADVLETLGISRPEKM"
     gene            complement(1745..2695)
                     /locus_tag="VFN58_03365"
     CDS             complement(1745..2695)
                     /locus_tag="VFN58_03365"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917062.1"
                     /translation="MLRTLDLLASDSSHLPFLYVFKESGVMEKYGFEIDLHIVGGAKA
                     PTMAHRAKLALAGEIDFLSGLHHETYRERAKGEKRLTYLAQAQNNWDDRLVALPKIQS
                     VQDLKGQKIVCHSTAPCVAGNLRAVLEECGLNPDEINIEVVKDSSGKFLHFVDQLTSG
                     EAVAALVDMPFDLYGIKKGLKIVDLPDRPVIHNTTLLATTDYIKQNEETVYAFIKGFI
                     EALHFFKTQPDKVVPILKKNLAKRYGLEEDEYYIHLQREWARLLSKKPYPLASAIQNV
                     YDLDVGKDPAMRNIGPMEPWDLHYLRAIDDSGFIDRLYAS"
     gene            2988..>3558
                     /locus_tag="VFN58_03370"
     CDS             2988..>3558
                     /locus_tag="VFN58_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET8917063.1"
                     /translation="MIPPTFDYHVPNSVNDAIGMLRRYGSDAKILSGGMSLIPLMKLR
                     LANPAYLIDINRISGLDHIKEADGFLRIGALVREADLEKSELIRSKYQLILDTTLVIA
                     DPLVRNRATIGGNLAHGDPANDHPATMLALEAEIAVVGPQVERRIPVTSFFTDLFTTA
                     LSADEILTEIRIPIPPARSGGAYFKLERKV"
BASE COUNT          795 a          928 c          869 g          966 t
ORIGIN      
        1 cgaccgtgag ttacatggga ttggtggtta ttctgctggc gattctggta gcgtggcgcg
       61 cccccgttgt tcgcgatatc gggcttggga cgaggagttg actttcgtgt tacgtgcttc
      121 ctgtgatccg tctcggttca catcttcttt atctctggta aattacctgg gcttaataaa
      181 gagacagtgc gatcgcctct ctttacattt tttccgggcg ggaaatgccg agtgtttcga
      241 gaacgtcagc cagagtttct tttgttttta aaaccaaata taaaagctcc gcttcgcggt
      301 ccgagccgat cactttcacg tcgcggtaaa acgcggaaaa tgcctgcgcc agctcgtagg
      361 catatgaagt taaacgctgg accccatagt cccgcgagat gtcgtcgaga atttccggat
      421 agcgaagaat cttgagagcc agttcgcgtt cagcttcatt cttaaaaact ttcagtggcg
      481 aggcccttcc ggtcgacttc tgtaaaatgc tcgccatccg cgtatgggca tattgaacat
      541 agtaaaccgg atttttctcc gatttctctt tcgccaagtc cagatcgaaa tccatgtgag
      601 tattggttcc ccgttcaaga aaaaaccacc gcgcggcgtc cacgcccact tcttcaagaa
      661 gctcgtccat agtcacaaat tcacccgcgc gttttgacat tcgcacttcc gtgccgccct
      721 gcatcaagcg gacaagctgc atgatgataa tcttgagacg ttcaggattt ataccaagcg
      781 ccgctattcc ttttttcatc cggtccacat agccgtgatg atccgctccc cacacgtcaa
      841 tggcaatatc aaattgtctt ttcaggaatt tgtcataatg atacgcaaga tccgagagaa
      901 aatacgtcgg ctcaccgtca gattttataa gcaccgcgtc gccgagccaa attgcgtcgt
      961 ctttcttatc aagcaatcct tttgattcta aaaactttaa aaccttctcc aactcacctt
     1021 gttcgcgaag tttttgctct gaaaaccatg tatcaaactc aattccggct tttttgagcg
     1081 aaagcttaat ctctaaaagc aatgtctgga ccgcttcttc ggcgtttttt ccggcaaggg
     1141 tttttacata ttcgcctttg tagtattctt cttttgcctc gcgttttccc tcggctgcct
     1201 cgatcgactc ccccaaaagc ctcacctgat ttccgctatc gttgatataa aactcgcggg
     1261 taacgttgta gcccactttc ctcaaaatcg ttgcaagcgc atccccgtaa aatccgccgc
     1321 ggccatttgc catcgtgagc ggccccgttg gattagcaga gacaaattca atattgatct
     1381 tttctttctt gaccggaaat tccgttttcg cggagagcgt tttgagtaaa acctcgtctt
     1441 tgacaaaaat attcagaaat ccaccctttg cttccgcgcg ctcgatcatt ttggaagacc
     1501 cactcaattt cgcggcggca tcagccgcaa tctcggccgg ttttcttgaa agtttctttg
     1561 caagaagaaa aggtgcattg gttgaccagt caccattctc acgacgctcc ggtttcgtca
     1621 ggtgcacacc ggattcggcg ccgagaactg ccgaaatttc tcttgcgaga tcatctctca
     1681 gcataaaaga acaagccctt agcgttttgt ctgccgagca gagtgaccag taaagctcgc
     1741 tcctttagga ggcgtagagc cgatcgataa agccactgtc atcgattgca cggagataat
     1801 gcaggtccca cggttccatt ggcccgatat ttctcatggc tggatctttc ccgacgtcga
     1861 ggtcgtagac gttctgaatg gcactggcca ggggataagg tttctttgac aacagtcgcg
     1921 cccactcgcg ctggaggtgg atataatatt cgtcctcctc gagtccgtag cgctttgcca
     1981 agtttttctt gaggatcggg acgactttat cgggttgagt cttaaagaag tggagggctt
     2041 cgatgaagcc tttgataaag gcatacaccg tttcttcgtt ttgtttgatg taatccgtcg
     2101 tcgctaaaag agtcgtattg tgaattaccg gccgatcggg tagatcgacg atcttcagtc
     2161 cctttttgat gccgtagaga tcgaagggca tgtccaccaa cgccgccacc gcttcgccgg
     2221 aggtcaactg atcgacaaag tgcaagaact tgcccgagga gtctttcacg acttctatat
     2281 tgatctcgtc cggattaagc ccgcactctt ccaacaccgc ccgcaagttg ccggcgacac
     2341 aaggtgctgt cgaatgacag acgatctttt ggccctttaa atcctgaacg ctttggatct
     2401 tcggcagcgc gacgagccgg tcatcccagt tgttctgggc ctgagcgagg taggtgagcc
     2461 gtttttcccc tttggctcgc tcgcgataag tctcgtgatg taggccgctg agaaagtcga
     2521 tttcgccggc taacgcgagc ttcgcgcggt gcgccatggt cggcgctttg gcgccaccga
     2581 cgatgtggag gtcgatttca aaaccgtatt tttccatcac cccagactct ttgaagacgt
     2641 agagaaaagg cagatgggaa gaatcactgg cgagcagatc gagggtacga agcatagatt
     2701 cactcctttt tatgattagt cttgattagg ctattcgatt aacctagaag cctgctgtcc
     2761 tgtattggat ttacttatca tatgttttcg ccttctaaaa agtccctccg ctttgtgtgg
     2821 attggcttgc tgacgcttgg actcttccgt tgtgacatca ggcgttttgc tcttgacaag
     2881 ctcatataga ggggacagac atgttgtaga tactggagta tctgtcgatt gctaccggtc
     2941 ccttaccatt ggtcccctgc gtgaattgac tttcaaggag cttcacgatg attcctccga
     3001 cctttgatta tcatgtgccg aacagcgtga atgacgccat cggcatgctt agacggtatg
     3061 gctccgacgc aaagatactg tcgggcggga tgagcctgat cccgctcatg aagttgcgcc
     3121 tggccaatcc ggcttacctg attgatatca atcgaatctc gggactggat catatcaaag
     3181 aggcggacgg cttcctcagg atcggggcgc tcgtgcgcga ggcggatctg gaaaaatcgg
     3241 agctgatcag aagcaaatac cagctgatct tggatactac cttggttatc gcggaccctt
     3301 tggtgcgaaa ccgcgccacg atcggcggaa atttagctca tggcgatcca gccaatgacc
     3361 atcccgcgac catgttggcg ctggaagccg agatcgcggt tgtcgggccc caagtggagc
     3421 gaaggatacc agtcacgagc ttttttaccg acctgtttac taccgccttg agtgcggatg
     3481 aaattctcac cgagatccgt attcccatcc cgccggcccg aagcggcggc gcctatttca
     3541 agttggagcg aaaggtcg
//
LOCUS       DASSWZ010000137         8660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_726024, whole genome shotgun sequence.
ACCESSION   DASSWZ010000137 DASSWZ010000000
VERSION     DASSWZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726024"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VFN58_03375"
     CDS             <1..1629
                     /locus_tag="VFN58_03375"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET8917064.1"
                     /translation="AIEDRERVGCLAKPFSPYELKEKVGQLLARRNILSETLSHPGVE
                     PQAEFTRYLEFPYLSRAATSLVHRFAATGLPILISGEIGCGQERVARGIHFLGARFGS
                     WILLHAAEINSAYLDQKSLQLSWCRRSEAPPVTLIIENVDQLSLSGQAELVNFLEQEE
                     AKFGKCRLITTARVDILEKVYRDELLESLYYRLATLKLSLRPLRERREDVPAIASWFC
                     QFYASYIGLGEVSLSVGANERLSNYLWFGNLNEMETVIARTLALQRKSWIEAADLIFE
                     FSVAGELPELPAFKELVRSGAQEKSELETAESLTRIRNGLHTEARTENSHAQREDLSV
                     LIHELAHELKNPMVTIKTFAQLLTDRYQDENFRARFQDVVGCDIERMDDLLELMIEFA
                     GFAQPRWNKVGLQEKLRSALNEVTNECARRQAQITWKGNGYSREIKADETQVKYVLKN
                     VLLAVLSQAKLASEIEINVEKQGCVAISYFREVGRVASITHYFDKSSSSPSETVLPLR
                     ILLAKQLVERNGGNMILDYADSEKDLLKMEFLIA"
     gene            1649..3055
                     /locus_tag="VFN58_03380"
     CDS             1649..3055
                     /locus_tag="VFN58_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8917065.1"
                     /translation="MSQPLLLVVDDEAGVRQSLQLVFNKMYRVLEATSADEAIQKLAD
                     EKPDVVLLDIVMPGADGLAVLKQMRSIHTDCQVIMLTALNTARTAFTAKGTGAFDYVT
                     KPFDVEELRLRVEHAFEKVHLFRELERLKEEVGRKYGVEHVIGRSKQIIDVFKAVSMV
                     AAKKSTVLITGESGTGKELIARAIHYNSDRRSKPFVVVNCAALPDTLIESELFGYEKG
                     AFTNASQKKVGRFELAHGGTLFLDEIGELNLGTQAKFLRAIEQETFTRLGGTDEIKVD
                     VRVIAASNRDLETMAKAGEFRPDLFYRLNVVSLYLPPLRERPEDIALLLDHFLRLKAQ
                     EHSMAPKSLSPEVVDFFTSYYWPGNIRELENLIERLTILTPHETIMLRDLPAGMRSTD
                     QTATLKEDVLSGSRPLSEAVDEFERELIVKALQRTGFNQTKAASLLGTSRRILKYRIE
                     KLKIHEPNDGGEEAKLST"
     gene            3176..4297
                     /locus_tag="VFN58_03385"
     CDS             3176..4297
                     /locus_tag="VFN58_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917066.1"
                     /translation="MDETKPHILVVDDEMGPRESLKMILNPHYTVHVAERGGQAVEML
                     GKFPVDLVTLDLKMPGLSGIHVLEKLKEYDPDIEAIIITGYGSLDTAIEGLRLGAFDY
                     ISKPFDVNHILSLVRRGLARRNAKSKLRQVKSDFLSNVSHELRTPLSVVVGFVYLLLN
                     QVIGKLSEEQQKVLETVYRNSEELLELIDNVLWMTSLNAGDATATIEKFDGREVVREA
                     VKRYERILDEKGLALSIEVADTGMSIISDRSKVERIFQNVLNNAIKFTSQGEIRIKAY
                     PAADHSSIEFDVTDTGIGIEKNKMDSIFEPFQQVDTSSHRSFSGLGLGLTVARRMAEL
                     IGGMLEISSEAGKGTRVLMKFPSQASAATSLLTEQRKYG"
     gene            4290..4991
                     /locus_tag="VFN58_03390"
     CDS             4290..4991
                     /locus_tag="VFN58_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917067.1"
                     /translation="MGSEKFIHCCVCDAIHHVSAFDKAPAYVFVRDEVEAQATDDWRL
                     FMAQHAGHRLEPLKASGEKLFPDGFPLDPMSAVYFEVTNGQDRYVVRRARKSIQEPMG
                     YELIRGRLADAGVTVEVQEREIKKEIKKHFSWAPATCPDDEKIDLFVGLVKEFAKALD
                     PQRIQISEYSYTDDSISYGTFDSTVLDALTERCAVYFLPDELASLRRFVDTHRCGSDV
                     MTLVLRRRLTIERVA"
     gene            complement(5222..5761)
                     /gene="pyrR"
                     /locus_tag="VFN58_03395"
     CDS             complement(5222..5761)
                     /gene="pyrR"
                     /locus_tag="VFN58_03395"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET8917068.1"
                     /translation="MKRPKSVRILNALDIKVNLERMARAILRQNKAMDSLGFIGIRTR
                     GLFLARRLCEIIKRSNGKDVPLGEMDITLYRDDLNALLPQGKVDHTQIPFDINNKTII
                     LFDDVLYTGRTVRAAVDHLIDLGRPRAIHLAVLIDRGGREFPIAANFIAKKINLPGDG
                     EVRLKLREVDGKDEVVVTK"
     gene            complement(5748..7046)
                     /locus_tag="VFN58_03400"
     CDS             complement(5748..7046)
                     /locus_tag="VFN58_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HET8917069.1"
                     /translation="MKSPQSLPASPLLPDIAINRRRLLRDLNAVSRIGIGDHGSVTRL
                     VFSIQELRSRQFLIHQMRQIGLQIHIDRIGNIFGRLDGHNAKAPALLVGSHLDTVLHG
                     GKFDGTMGVIAALEAARTLKEQEIVLQSPVEVVCFVGEESSRFGYSTLGSSLVAGEVH
                     AKDLDYAADAQGNKLADILASMGIYRNNLRSMRRDPSTVKAYLELHIEQGPILEAKKK
                     PIGIVTSIAAPTRFRVIFTGQADHSGTTPMEMRKDALVAASELIVAVEEICCRYSHME
                     KGRVVGTVGAMKIEPGVINAIPGKAELAVDIRSITADAKKRVVRLVQAQIREIARRRK
                     IPAEILPLREEDPVPLDKRLIRLLQECCESRNIAYEIMPSGAGHDAMQMAKVAPAGML
                     FIPSRRGISHSPLEWSEPEDICLGAQLLLDSIIRIGNETT"
     gene            7175..7843
                     /gene="pyrE"
                     /locus_tag="VFN58_03405"
     CDS             7175..7843
                     /gene="pyrE"
                     /locus_tag="VFN58_03405"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET8917070.1"
                     /translation="MSTVAQKIAEMLLRTEAIQVYKENPFVFVSGRISPVYIDCRKIL
                     SFPEEREYIVAQLAKKTETDIGLDNIDVVAGGETAGIPYASFVSHLIKKPMIYIRKQP
                     KGYGGTKQIEGILEPGKRVLLVEDLITDGLSKLRFNIGIRAAGAKMTHCLCVFDYASD
                     RLKQHEGRDNLAKNDIGLHVLANWDDVLDTGFSKSYFSEDASKQIVDFLNDPENWGRK
                     MGFV"
     gene            7998..>8660
                     /locus_tag="VFN58_03410"
     CDS             7998..>8660
                     /locus_tag="VFN58_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917071.1"
                     /translation="MKTLIRNALIVTMNRENDVIESGSIVIDGNRLAYVGPTEWTPPG
                     PFDRAIDGDLRIAMPGMVNAHCHSPANLVRGMMPSKPLEIWRAYYRASLRDMRDEDFY
                     ASALLGGMEMLKNGATTVLDHFFGNQNCRFMGAGAAIQAMRDLGLRHVISLTLSDKNY
                     EETIPLGETALNLTEEIKRMSASEGKATEAWLDDCGDFIDTFHDPDRLTTCCPGPSAV
                     QRC"
BASE COUNT         2089 a         2147 c         2348 g         2076 t
ORIGIN      
        1 gcgatcgagg accgagaaag agttggctgt ttagccaagc cctttagccc gtatgaattg
       61 aaagagaagg tggggcaact cttagcccgc agaaacattc tatccgaaac tctatcccat
      121 cctggtgtcg agcctcaggc agagtttacc cgatacctag aattccctta tcttagtcga
      181 gccgcgacca gcctggttca tcggtttgca gccacgggtc tacccatttt gatctccggg
      241 gagatcggct gcggccagga acgcgtagct cgcgggattc attttttggg agcgcgtttc
      301 ggcagctgga ttctattgca tgcggctgag attaactccg cctatttgga tcaaaaaagc
      361 cttcagctgt catggtgtcg tcgcagcgaa gcgccgcccg ttacattgat aatcgagaac
      421 gttgatcagc tttcgctatc cggccaagcg gaactcgtga attttctaga acaagaggag
      481 gccaaattcg gcaagtgccg tcttatcaca acggcgcgag tcgatatctt ggagaaggtt
      541 tatcgggatg aattactgga atctctctac tataggttgg ccacattaaa attgtctctg
      601 cggcccctac gggagcgacg ggaggatgtg ccggcaatcg ctagttggtt ttgccaattt
      661 tatgctagct atatcgggtt gggagaagtc agtctttccg taggtgccaa cgagcgtctc
      721 agcaactacc tttggttcgg gaacctaaac gaaatggaaa ccgttatcgc ccgaacactg
      781 gccttacaac gaaagagctg gatcgaagcc gccgatctga tttttgaatt ttccgtcgcg
      841 ggagagttgc cggagctgcc cgcgtttaag gagttggttc gttcaggggc ccaagaaaaa
      901 agcgagctcg aaacggccga aagtctgacc cggatccgca acggcttaca cacggaggcc
      961 agaacggaga atagtcatgc ccaacgggaa gacttaagtg ttttgatcca tgagctagcc
     1021 catgaattga agaacccgat ggtcacgatt aagacctttg cgcagttgtt aactgaccga
     1081 taccaggacg agaatttccg ggcccgtttc caggatgtcg tcggttgcga tatcgagcgg
     1141 atggatgacc tgcttgagct gatgatcgag ttcgctggct tcgcccagcc gcgttggaac
     1201 aaggtagggt tgcaggaaaa acttcgctca gcgttgaatg aagttaccaa tgaatgcgcc
     1261 cggcggcaag cccaaatcac ttggaaaggg aacggctata gccgtgaaat caaagccgac
     1321 gaaactcagg tcaagtacgt tctgaaaaac gtgctattgg ccgtattgtc gcaggcaaaa
     1381 ctggcaagcg aaattgagat caacgtcgag aaacaaggat gcgttgcgat ctcatatttt
     1441 cgtgaggtag ggcgtgtcgc gtcgatcacg cattatttcg ataaatcgtc ttcgagcccg
     1501 agcgaaaccg tactcccctt acgtatcctg ctcgcaaaac aactggtaga gcgcaacggc
     1561 ggcaacatga ttctcgatta tgcggacagc gaaaaagacc ttttgaaaat ggagtttctg
     1621 attgcttgaa ttagaaaaga agcgagaaat gagccaaccc ttattgctag ttgtcgatga
     1681 cgaggctgga gtgaggcaat cgctgcagct ggtttttaac aagatgtatc gtgttctaga
     1741 agccacgtcg gctgacgagg cgattcagaa gctcgcggat gaaaaacctg acgtcgtttt
     1801 attggatatt gttatgccgg gtgcagatgg tctggccgtc ctcaaacaaa tgaggtcgat
     1861 ccataccgat tgtcaggtaa tcatgttgac tgctctcaac acggcgcgaa cggcctttac
     1921 agccaaaggc acaggtgctt tcgattatgt gaccaagccc ttcgatgtgg aagagctgcg
     1981 cctgcgagtc gagcatgctt ttgagaaagt tcatttattc agggaactgg aacggttaaa
     2041 ggaagaggtc ggccgcaagt acggagttga gcacgtcatc gggcgatcca aacagatcat
     2101 cgacgtgttt aaggcggtca gcatggtggc ggccaagaaa agcacggtgc tcattacggg
     2161 cgaaagtgga acggggaagg agctgatcgc gcgtgcgatt cactataata gtgatcggcg
     2221 ctccaagccg ttcgtcgtgg tcaactgcgc ggctctcccc gacactttaa tcgagagtga
     2281 attgttcggc tacgagaaag gcgcttttac gaacgcttcg cagaaaaagg tggggcgctt
     2341 cgaactcgcc catgggggaa cgctttttct cgacgaaatc ggtgagctta atctggggac
     2401 tcaggcgaag tttctacgcg ccatcgagca ggaaaccttt acccgtctcg gtggcacgga
     2461 tgaaatcaaa gttgatgtcc gagttatcgc cgccagtaac cgcgatttgg aaacgatggc
     2521 caaggccggg gagttccgtc ccgatctttt ctaccggcta aatgtcgttt cgctttacct
     2581 ccctcccttg agggaaagac ccgaagatat agcactgctc ttggaccatt tcttgagact
     2641 caaggcgcag gaacattcta tggcccccaa aagcctgtct cctgaggttg tcgacttttt
     2701 tacttcatac tactggcctg gaaacattcg ggaactggag aacctgatcg aaaggctgac
     2761 gatcctaacg ccccacgaaa cgatcatgtt gcgggatctg ccggcgggga tgcgttccac
     2821 ggatcagact gcgacgctca aagaggatgt actgagtggt tcgagaccgt taagcgaagc
     2881 tgtagacgaa tttgaacggg agcttatcgt taaggctctg caacggaccg gattcaatca
     2941 gaccaaagcg gcttcacttc taggaaccag ccgtcgtatt cttaaatacc gcatcgaaaa
     3001 gctaaaaatc cacgagccca acgatggagg tgaagaagcg aaattatcga catagttgga
     3061 caaaattgtt cccttaactc tctctattcc tttccttggc ttaaaattac gcttgtaaat
     3121 tcgtgatttg gctttgtgtg gcaattggca tgttgtttgc tcgataaggg ctgacatgga
     3181 cgaaaccaag cctcatatcc ttgtcgtcga cgacgaaatg ggtccgagag aatcattaaa
     3241 gatgattctc aatccccatt atacagtgca cgttgcggaa cgcggcggac aagcagttga
     3301 aatgctcggc aagtttccgg ttgatttggt aacgctggac ctaaaaatgc ccggtctctc
     3361 cggaatccat gtgctcgaaa agctgaagga gtacgatccg gatatcgaag cgataatcat
     3421 taccggatac gggtctctgg atacggctat cgaggggttg cgcctgggtg cgtttgacta
     3481 tatttcgaag ccctttgacg taaatcatat tctatctctg gtgcgccgcg gccttgcgcg
     3541 ccgaaatgcg aagagcaaat tgagacaggt caaatccgat tttctctcca acgtttcaca
     3601 tgagctgaga actcccctca gtgtggtggt gggttttgtc tacctactct tgaaccaagt
     3661 cattggcaaa cttagcgagg agcaacaaaa agttttggaa accgtctacc gtaactccga
     3721 ggagttattg gaactcatcg ataacgtgct ctggatgact tctctcaacg ccggcgatgc
     3781 gacggcgacc atcgaaaaat tcgacggccg tgaagtagtg cgtgaggcgg ttaagcggta
     3841 cgagcgtatc ctggatgaga aaggactggc gctctcgata gaggtggccg atacgggaat
     3901 gtcaatcatc agcgaccgct ctaaagtcga aaggatcttt cagaacgtcc ttaacaacgc
     3961 gatcaagttc acatctcagg gggaaattag gatcaaggct tatcctgcgg ccgaccattc
     4021 gagcatcgag ttcgacgtta cagacacagg tatcggtata gaaaagaata agatggattc
     4081 gatctttgag ccgtttcagc aggtcgacac atcgtctcac cgttccttct cggggttggg
     4141 tttggggctc actgtcgcgc gccgaatggc tgaactcatc ggtggaatgc tggaaatcag
     4201 cagtgaagct ggcaaaggca cccgagtgct gatgaaattc ccttcccagg cgagtgccgc
     4261 cacttcgctg ctgacggagc agcgtaaata tgggtagtga aaagtttatc cattgctgcg
     4321 tctgtgacgc tatccatcac gtttctgcgt tcgataaagc tccggcctat gtttttgtac
     4381 gggatgaagt ggaagcgcaa gcaaccgatg attggcggtt gtttatggcg caacacgctg
     4441 gacatcggct agaaccgctc aaggctagcg gtgagaagct cttccccgat ggcttcccct
     4501 tggatccgat gagcgcggtt tattttgagg tgaccaacgg tcaggaccgt tatgttgtgc
     4561 ggcgcgcgag aaagagcatc caggaaccca tgggctatga attgattcgc ggtcgcctgg
     4621 cggatgctgg cgtaacggtt gaggttcagg agcgcgaaat taagaaagaa atcaagaaac
     4681 acttttcctg ggcacctgcc acttgccccg atgatgagaa aatcgacctg ttcgttggat
     4741 tggtcaagga attcgccaaa gccctcgatc cacaacgcat tcaaataagt gaatactcat
     4801 atacggacga ctcgatttcc tacgggacct ttgacagcac cgttctagat gcccttacag
     4861 agagatgtgc cgtttatttc ttgcctgacg aactcgcttc acttcgtcgg ttcgtggaca
     4921 cccaccgttg cggttccgat gtcatgaccc tagtcctgcg ccgtcgatta accatcgaac
     4981 gggttgcctg atcgttggct gtttttcact ccagagccct atcttgctgg tttcgtccta
     5041 ttttcgtggg ttgagggtgg tttttacacc atagtgtctt agcgacggac atggacatta
     5101 ctgaatcgtc cccgtcgatc gatcacgcat aaaatctcac gtgagtcctc caaggcggct
     5161 tttgccgtat catagcctgc ttgccgtttc ttcaaatagc cctggaagga tcagattagc
     5221 gtcacttagt gactacgact tcgtctttcc catccacttc cctaagtttc aatctgacct
     5281 cgccatcccc cggcaagttg atcttcttgg cgatgaaatt ggcggcaatg ggaaattcac
     5341 gcccaccgcg atcgatcagc accgcgagat gaatagctct aggccggcct agatcgatga
     5401 gatggtccac cgccgctcgt acggtccggc ccgtgtacaa tacatcgtcg aacaagatga
     5461 ttgtcttgtt attgatatcg aagggaattt gcgtatgatc taccttgcct tgaggcagga
     5521 gagcattgag atcatctcgg tagagagtga tgtccatctc accaagaggc acgtctttgc
     5581 cgttagacct tttgattatc tcacacaacc gtctggcgag gaaaaggccg cgggtacgga
     5641 tgccaataaa tccgagcgag tccatcgctt tgttttgacg aagaatggca cgggccatcc
     5701 gctcgagatt taccttaatg tccagagcgt tcaggatccg aacgctttta ggtcgtttca
     5761 ttgcctatcc ggattatgga atccaatagt agttgcgcac ccaagcagat gtcctctggc
     5821 tcggaccatt ccaagggact atgactgatg ccgcgccggc tgggaatgaa caacatcccg
     5881 gcaggcgcaa ctttggccat ctgcatagca tcatgaccgg ctcccgaagg cataatttca
     5941 taggcgatat ttctagactc acagcactct tgtagcagac gaatgagccg tttatccaag
     6001 gggaccgggt cttcttcacg aaggggcaag atttcagctg ggatcttcct gcgccgtgcg
     6061 atttcgcgaa tctgagcttg aaccaaccgg accacgcgtt tcttagcgtc tgcggtgatg
     6121 ctgcggatgt ctactgcaag ttcggccttt cctgggatag cgttaatgac tccgggctcg
     6181 atcttcatcg cgccgacggt accgaccacc cggccttttt ccatgtgcga atatctgcaa
     6241 caaatttctt ccacagcaac gatgagctca gacgcggcca caagagcgtc tttgcgcatc
     6301 tccatcggcg tggtaccgga atgatcggcc tgaccggtga agatgactct aaatcgcgtt
     6361 ggggctgcaa tggaggtaac gatgccgatg ggttttttct tagcttccaa aatcggtcct
     6421 tgttcaatgt gcagttcgag ataggctttt acggttgacg ggtcgcgacg catggaccgc
     6481 aggttgtttc ggtagatgcc catgctcgct aaaatgtccg ccagtttatt tccttgggca
     6541 tccgcagcat agtcgagatc cttggcgtga acttcccctg cgactaagct gcttcccaga
     6601 gtcgaataac cgaaacggct cgattcttca ccgacaaagc aaacaacctc tacaggagat
     6661 tggaggacaa tttcttgttc cttgagggtc cgcgccgctt cgagtgcagc aatgacgccc
     6721 atggtaccgt cgaacttgcc gccatggaga accgtgtcta gatgcgaacc gacgaggagt
     6781 gcgggcgctt ttgcgttatg gccatccagc ctgccaaaaa tattgccgat ccggtcgata
     6841 tgaatctgca aaccgatttg ccgcatttgg tgaatgagaa attgccggct gcgcagctct
     6901 tgaatggaaa agaccagtct tgtcaccgag ccgtgatcgc cgatcccgat acgtgacacc
     6961 gcattgagat cgcgcagcag acggcgcctg ttgatcgcga tatcgggtaa aagaggcgat
     7021 gctgggagac tttgcggcga tttcacaaaa cgtattctac cagtttaact gcggcgataa
     7081 aaaccgtgct cgtcgaggtc gatcctcatg ttggaaatgg gaaaaggcta tgttatagcc
     7141 tgcctattcc agagatgggg gagggtaatt ttctatgagc accgttgccc agaaaattgc
     7201 agagatgctg cttcggacgg aagcgattca agtctacaag gaaaatccgt ttgtcttcgt
     7261 gtccggccgc atttcgccgg tctatatcga ctgccgtaaa atcctttcgt ttcccgaaga
     7321 acgcgagtac atcgttgcgc agctcgccaa aaaaacggaa accgacatcg ggttggacaa
     7381 cattgatgtc gtcgctggag gggagacggc gggtattcct tacgcgtcat tcgtctccca
     7441 tctcatcaag aagcccatga tttatatccg taagcaaccc aagggctacg gcggcacgaa
     7501 acagattgaa gggattctag aacccgggaa gagggttttg ttggttgaag accttatcac
     7561 ggatggactg agcaagctgc ggttcaatat cggcatccgt gcggcgggag cgaagatgac
     7621 gcactgtctg tgcgtttttg actacgcctc ggaccgttta aagcaacatg agggcaggga
     7681 taatctggcg aagaatgata tcggcctgca cgtgcttgcc aattgggacg acgtactcga
     7741 taccggtttc agcaaaagct acttctctga agatgccagc aagcagattg tcgattttct
     7801 taacgacccg gagaattggg ggcgcaagat gggctttgta tgagcgcaat tttgcgtacg
     7861 gttccggggt tcgcgttttt gagttcaccc gataacggaa aacgaggaac ctaaacgatt
     7921 ttccctacct attagaactg ggtgtagctg cttgtttccc ggcggttcga cttcaattca
     7981 tactgacgag ttgcgccatg aaaactttga ttcgtaacgc tctcatcgtc acgatgaacc
     8041 gagagaacga cgtaatcgag agcggttcga tcgttatcga cggtaacagg ctggcgtatg
     8101 tgggacccac tgaatggaca ccgcctggtc cgttcgaccg cgccatcgat ggcgacctgc
     8161 gaatcgcgat gccgggcatg gtgaacgctc actgccactc gccggcgaat ctcgtgcgcg
     8221 ggatgatgcc gagcaagccc ctggagattt ggcgcgccta ctaccgcgcc tcccttagag
     8281 atatgcgcga tgaggatttc tatgcgagcg ccctgcttgg aggaatggaa atgctcaaga
     8341 acggcgcgac cacagttcta gatcactttt tcggcaatca aaactgccgc ttcatgggtg
     8401 cgggcgccgc aattcaagcc atgcgcgatc tcggtttacg ccatgttatt tcgctcacgc
     8461 tttccgacaa aaactacgaa gagacaatcc cattgggcga aacggctttg aacctgaccg
     8521 aagagatcaa acgcatgagc gcgagcgaag gcaaggcgac cgaagcctgg ctcgacgact
     8581 gcggggactt catcgatacg tttcatgacc cggataggtt gacgacttgt tgtccgggac
     8641 cgtcggcagt ccagcgctgc
//
LOCUS       DASSWZ010000138         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_711987, whole genome shotgun sequence.
ACCESSION   DASSWZ010000138 DASSWZ010000000
VERSION     DASSWZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711987"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFN58_03415"
     CDS             <1..302
                     /locus_tag="VFN58_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917072.1"
                     /translation="LKAYVEGTRRLLTDRELGIRALRKYGGVEDRELLNTTYDLFTTR
                     YTKKVPKVNPKAVETALGLLAENNPKAKGRKAEEFMDTSFMDELEKIGFIKSVWP"
     gene            complement(335..745)
                     /locus_tag="VFN58_03420"
     CDS             complement(335..745)
                     /locus_tag="VFN58_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET8917073.1"
                     /translation="MADKGVFVLDTSALLALRGDEPGADRVENLLSKSKSNTCILLIS
                     FMSRMELLYLVWREEGEEAAREALRLIDSFEIVWVSCEAAILELASRLKAQGGLSVAD
                     SWIGATAIAREATLVHKDPEFAKFKEISQELLRK"
     gene            complement(738..968)
                     /locus_tag="VFN58_03425"
     CDS             complement(738..968)
                     /locus_tag="VFN58_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET8917074.1"
                     /translation="MRTKVTKRGQVSVPSEVRRRLRIGPDTTLEWVVEGATARVIPLP
                     ADPLKAFRGSGKKGMVKRLLEDRQRDRQRENG"
     gene            1162..>2801
                     /locus_tag="VFN58_03430"
     CDS             1162..>2801
                     /locus_tag="VFN58_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8917075.1"
                     /translation="MTELARTASQAYLEVAAAHGVDYVFGLPGTSGQEFIGTIADQEK
                     VRFILALHETCVVSMADGYARVTGRPSLAQLSTLPGTANSVGALYDAYRDRSPVVLTS
                     TNVDTRIVGRDSHTEGKDLVELTKQFTKWSCEVHRADRIPEILNRAFKVASTPPPGPV
                     YLSLPSNLLGESISVQNPDVERSRIVSRMAGDPDALKDAAKLLAQAKRPLIVAGSGVA
                     KAGAIEDLVELAELAAAPVVMEPRYSFLSFPTTHPYSFQIAERQPTFNLPVWGEPDLI
                     FAIGCRLIREYRYIPDPVIKTETRCVHIEEDPWEIGKVFPVDLGIVADPKSALRSLLE
                     IFPMFSEQSNGSRKTERIECLQKAKAQTNAELEGRVKQGWDATPINAARLMRTMDKLI
                     DQDALIVNESPTSKDILMSNFQFTCARSYFSNSSAGHLGWGLGAAIGAALASPKRRVV
                     ACLGDGSAMFGIQGLWTLAKYRVPLLVIVFNNRAYMAVKNQFRGPEERIRIAAEMGAE
                     LVGPEINFSRLAETFGIFGQRVEQPEAIEPALKRALEQS"
BASE COUNT          629 a          766 c          786 g          620 t
ORIGIN      
        1 tcctcaaggc ttatgttgaa gggacccgcc gcctgctaac cgacagagag ttgggcatca
       61 gggcactgag aaagtacggc ggcgtcgagg acagagagct gcttaacacg acctatgatc
      121 ttttcacgac ccgttacaca aaaaaagttc cgaaggtcaa tcccaaggcg gtggagacgg
      181 cgttgggcct ccttgccgag aacaatccca aagccaaagg ccgcaaagcg gaagaattca
      241 tggacacaag ttttatggac gaactggaga aaatcggttt catcaagtcg gtgtggccgt
      301 gagccggcga ccatcagact tgcgagcaac cagactattt cctgagaagc tcctgggaaa
      361 tttctttgaa ttttgcgaat tctggatcct tgtgaactag cgttgcctcg cgcgcgattg
      421 ccgttgcgcc aatccagcta tcagcgaccg ataaccctcc ttgggctttt aggcgggagg
      481 caagctcaag aatcgccgcc tcgcaagaga cccatacaat ctcaaaggaa tcgatcagcc
      541 gcagcgcctc acgggccgcc tcttcaccct cttctcgcca gaccagatat agcaactcca
      601 tccggctcat gaatgagatg agcaagatgc aggtattgct ttttgacttg gagagtagat
      661 tttccacccg atcggcgccg ggttcatcgc cccgcagagc gaggagcgcc gaagtatcca
      721 gcacgaaaac acctttgtca gccattctct ctttgtcgat cgcgttgccg gtcctctagg
      781 agccgcttca ccattccttt tttgcccgag ccgcgaaagg ctttcagagg atcggccggt
      841 aacggtatta cccgtgcagt ggcgccttcc acaacccact ccagggtcgt gtcaggacct
      901 atgcgaagcc ttcgtctaac ttctgacgga accgaaactt gtccgcgttt tgtcactttg
      961 gtacgcatat atgcatttat acaagattaa tgaagatttg acaagcaagt ttagatttcg
     1021 tcatgtagta tggatgaatc aagattgccg gcgttagcac tcctgcatcg gcagtaaggt
     1081 ggatcgaatc taacctttct ggtaaggtct tggcggccaa ggagacagag cttggattgg
     1141 caagagagga gaatccttta gatgacggaa ctagcacgca cagcatcaca ggcctatctg
     1201 gaagtggcgg cggctcatgg tgtcgattac gtattcggcc tacccggtac cagcggccaa
     1261 gaatttatcg gtacgatcgc ggatcaggaa aaagttcgct ttattctcgc gctccatgag
     1321 acctgcgttg tctctatggc tgatggctat gcgcgagtca cggggcggcc aagtctcgcg
     1381 cagttgagca cgcttcccgg gacggccaac tccgtgggcg ctctttacga tgcttaccgg
     1441 gaccggtctc ctgtcgtgct gacctccacc aacgtcgata cccggatcgt cggccgcgat
     1501 tcgcacaccg aaggcaaaga tctggtcgag ttgaccaagc agttcacaaa atggagctgc
     1561 gaggtccacc gcgccgaccg tattccggag atattgaatc gggcattcaa agttgcttcg
     1621 acaccacctc ccggtccggt ttatctctct cttcccagca atcttctcgg cgagtccatc
     1681 agcgtgcaga atcctgacgt ggagcggtcc cgcatcgtgt cacgcatggc gggtgatcca
     1741 gatgcgctga aagacgcggc caaattgctt gcccaggcca agcgaccgct gatcgtcgca
     1801 ggcagcggtg tggccaaggc cggtgctatc gaggacctag tcgaactagc ggagctagcg
     1861 gcggcgccgg tggtcatgga gccacgctat tcttttctta gctttccgac gacgcatcct
     1921 tacagttttc agatcgcgga acggcaaccc actttcaatc ttcccgtctg gggcgagccg
     1981 gatctgattt ttgcaattgg ctgccggttg attcgagagt accgctacat tcccgacccg
     2041 gtgatcaaga ccgagacccg ttgcgttcac atcgaggagg atccttggga gatcggcaaa
     2101 gtgtttcccg ttgatttggg aatcgtggcc gaccccaaga gcgcattacg ctcccttctt
     2161 gagatctttc ctatgttctc cgagcaatcc aacggcagcc gcaaaacgga acggattgaa
     2221 tgcttgcaaa aggctaaagc ccaaacgaac gctgagttgg aagggcgagt taagcaaggc
     2281 tgggacgcaa cgccgatcaa tgcggcgaga ttaatgcgga cgatggacaa gctcatcgat
     2341 caggacgccc tgattgtcaa tgaaagcccc acgagcaaag acattctgat gtcgaatttt
     2401 caattcactt gcgccaggtc ctatttttcg aactcatccg cgggccacct gggatggggg
     2461 cttggcgcgg cgatcggcgc ggcgctggcg tctcctaaac gacgggtggt ggcttgcctc
     2521 ggggacggca gcgccatgtt cggcatccag ggattgtgga ccctcgccaa gtatcgagtg
     2581 ccgctcttgg tgatcgtgtt taacaaccgc gcttacatgg cggttaagaa ccagttccgg
     2641 gggccggagg agcgcatcag aattgctgca gagatgggtg cggagcttgt gggtcctgag
     2701 ataaattttt cccggctggc agagaccttc ggcatttttg gtcaacgggt ggaacagccg
     2761 gaggcgatcg agccggcatt gaaacgcgcc ctcgaacaat c
//
LOCUS       DASSWZ010000139         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_188375, whole genome shotgun sequence.
ACCESSION   DASSWZ010000139 DASSWZ010000000
VERSION     DASSWZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188375"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..1929)
                     /locus_tag="VFN58_03435"
     CDS             complement(397..1929)
                     /locus_tag="VFN58_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET8917076.1"
                     /translation="MPANLDIEKTPDEIEDSREIELDTLGDKEPELTDVDSSDESQAE
                     EPAEREETGANFSSVQQYLHEIGSVALLNRERETELAMQMERGKNQILQALWSIPTAL
                     RHVLELGNAVASGDLDLRTVIDKADSDADDGEEILDPRPFLKLVGKLRKLHESQEQYR
                     RELKRSRLSKQRLALLTEKQSRLTEKVCAVMKELRLSSSRVEEMVQQLKRSAERLVAL
                     EQLEGTSSRGNRNTILAEKQIIEEKIGLPAAEIKSQVRLIQDGEALVSTAKKEFTEAN
                     LRLVVSIAKKYINRGLGFLDLIQEGNLGLMRAVEKFDYRLGFRFSTYASWWIRQGITR
                     GLIDTGRTIRIPVHRVELRNKIIQTAQRLQRKLDREPRPDELAKAMRMPVSDLLRVIQ
                     VQGEPVSLQTPVWEDGDELEDFVEDRINRRPDDEAIEGSLRRDVRKALAILTPRQEKV
                     LRMRFGIEEKRDYTLEELGEMFTVTRERIRQIEQKSLQILRNPNRRRPLQSARIVDTV
                     AV"
     gene            complement(2434..>3489)
                     /locus_tag="VFN58_03440"
     CDS             complement(2434..>3489)
                     /locus_tag="VFN58_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET8917077.1"
                     /translation="SNGNIETTQSSVAIRAKKAVILATGGSTGNINFRRMFDPRLTEV
                     LQLAGEPYTFQDGSGELAAMAIGASLWGFANQILENGDNIRTKRALATKYNYMTWALE
                     TPIFPLVRATGLDVKDWQDLILVNQVGKRFYDETKGDYPHGNVYDEVKPYTPNDYRNN
                     ENIKFSPTKYNFFNAAVAMNEYSEPPDYSAGPVWAIFDADAVEREKWKVTPPYVDPDG
                     YFFSAKTLPELAAAIKNPYQAKPMNAAALQAAVDRYNSFVDSGTDLDFGKPTPKYKIQ
                     TAPFYAAWATPLVHDSRAGLRINAKCQVMDMNGQVIPGLYCGGESAGGFNQHGLGRCT
                     TQGYIAGKNAAAETPEE"
BASE COUNT          708 a          949 c          949 g          883 t
ORIGIN      
        1 gagaagggct ttatcgacgg taggctcaat ctatgcggaa atttaacgcg tcgactttgc
       61 cgcccactat ccagagaggc tttcgcaatc atattaactt cgattgacga agtcccccac
      121 ccctggcgga gtccttcaag cgctacggct gcaatgcgac ctatttcttt tattctgacg
      181 aagtgctcac ttacatcagc gctcaaagtc cgcgccgatc ttcgcaggct atgaaggcaa
      241 gtttgtggcc gatcacgccg aaagatcgag caactaagca tcgaaagttg gcagggtgct
      301 gagaaaaccg ttagcctatg ggtttatcgg agaggtgaac gggtcagacg tgaaattcgt
      361 gctggtcgac ccccccattt tcagcagtct ggcgatctaa acggccacgg tgtcgacaat
      421 gcgggctgat tgaagcggtc tccggcgatt agggttgcgc agtatctgta gcgacttctg
      481 ctcgatctga cgaattcgct cacgggtaac ggtaaacatc tccccgagct cctccagcgt
      541 gtagtcgcgc ttctcctcga taccgaagcg catgcgcaat accttttcct gtctcggggt
      601 cagaatagcg agggccttcc tcacatctct acgcagcgaa ccttcgatgg cctcgtcgtc
      661 gggacgccta ttgatccggt cctccacaaa gtcttcaagt tcgtcgccat cctcccaaac
      721 cggggtttgc agtgagaccg gttctccctg aacctggatg actctaagta agtctgagac
      781 cggcatgcgc atggctttgg cgagttcgtc aggcctcggc tcgcgatcga gtttgcgctg
      841 cagccgctgg gcggtctgga tgattttgtt gcgcagttcc accctgtgga ctggaatgcg
      901 aatagtgcgc cccgtgtcga tcaggcccct cgtgatgccc tggcgaatcc accaactcgc
      961 ataggtggaa aaccggaagc caagccggta atcgaatttc tcgaccgctc gcatcaatcc
     1021 gaggttgccc tcttgaatga ggtccaaaaa gcctagaccg cggttgatat attttttggc
     1081 gatgctgacc accaggcgca agttggcttc cgtaaactcc ttctttgcgg tgcttacaag
     1141 agcttcaccg tcttggatga gacgtacttg gctcttgatc tccgccgccg gaaggccgat
     1201 cttctcctcg atgatctgtt tctcggccaa tattgtattt cgatttcctc tcgatgaggt
     1261 tccttctagc tgttccaggg caacaagccg ctcggcggaa cgcttcaact gctgaaccat
     1321 ttcttcaacc cgactcgacg agaggcggag ttccttcatc acagcgcaga ctttttctgt
     1381 tagacgcgat tgtttctcag tcaatagcgc cagccgttgt ttcgacaaac ggctccgctt
     1441 tagttcccgc cggtattgtt cttgactctc atgtaacttt cgtagtttgc cgaccagttt
     1501 gaggaagggt cttggatcga gaatttcctc gccatcatca gcgtcgctat ccgctttgtc
     1561 tataaccgtg cgtagatcga ggtctccgct tgcaacagca ttccccagtt caagcacgtg
     1621 acgcaacgcc gtcggtattg accagagcgc ctgaagaatc tgattcttgc cccgctccat
     1681 ttgcatcgcg agttcagtct cgcgctcccg gttgagcaga gcaacggagc caatctcgtg
     1741 caggtattgt tgaacggagc taaaatttgc tcctgtctcc tcacgttcag caggctcttc
     1801 cgcttgagat tcatcacttg aatcgacgtc agtaagctcc ggctccttgt cacctaatgt
     1861 atccagttcg atttcgcggc tgtcttcaat ctcatccggg gttttctcta tgtccaagtt
     1921 tgccggcatt gccatctcct tttaaccgca gccgtctaaa acacctttgc catttttcca
     1981 acgcaaaacc cgttctatct tagtcagtta gacgcgggat ttttggttgg gttggtagga
     2041 ccagcagaaa cgaaatccca ggccagaatc aaggttgata tggttaggaa aggtgattaa
     2101 ggcgagaatc gcgtctacga caggtccaat aacgcacttt ttaagataag accgccacgc
     2161 ggatgtgtca aggcaggtaa catgggtttt cgttacctct atgcctttgc aagccgagag
     2221 gtggctgcgg caatttcctg ggcgtgagaa aatatcgagg gtgattcgat atttaggtac
     2281 ggtgtcccaa cgttaatcca gcaaaatcaa ttgagtatcg gaatcgggat ttttcaactg
     2341 gggatcgacc aacgcaagca gttgatttaa tgaggttcgc tgaatgcggc ttcggtgcgc
     2401 caggataggc gagctttgcg gcggaaaggg ttcttactcc tcgggtgtct cagcggcggc
     2461 gtttttgccg gcgatgtagc cctgggttgt gcatcggccc aggccatgtt gattaaatcc
     2521 accggccgat tcccctccac agtagaggcc cggtatgact tgcccattca tgtccatcac
     2581 ctggcacttc gcgttgatcc tgaggccggc gcggctatcg tgcacgagcg gcgtggccca
     2641 ggcggcgtaa aacggcgccg tctggatctt atacttgggc gtaggcttgc cgaaatccaa
     2701 gtccgtgcca gagtccacaa aagagttgta gcgatcgacc gctgcttgca gcgcggcggc
     2761 gttcatgggt ttcgcctgat atggattttt gatggcggcg gctagctccg gaagcgtctt
     2821 ggcgctgaag aaatacccgt cgggatcgac gtaggggggc gtaactttcc acttctcccg
     2881 ctccaccgcg tcggcgtcga atatggccca caccggcccg gcggaatagt cgggtggttc
     2941 cgagtactcg ttcatcgcca ccgcggcgtt gaaaaagttg tacttggtgg gactgaactt
     3001 gatattttcg ttattgcgat agtcgttggg cgtgtagggc ttgacttcgt cgtacacgtt
     3061 gccgtgaggg taatcccctt tggtctcgtc gtagaatcgc ttgccgactt gattgaccag
     3121 gattaaatcc tgccaatcct tcacgtccaa gccggtggcg cggacgagcg ggaaaatcgg
     3181 ggtttcaagc gcccacgtca tgtaattata cttcgtggcg agcgcgcgct tagtgcgaat
     3241 gttatcgccg ttttcaagaa tctggttcgc gaagccccag agcgaggcgc cgatagccat
     3301 ggcggcaagc tcgccgctcc cgtcttgaaa cgtatagggc tcacccgcaa gttgcagcac
     3361 ttcagtcaac cgcggatcga acatgcggcg gaagtttatg ttgccggtgc tgcccccggt
     3421 ggcgaggatc acggccttct tcgcgcggat agcgacgctc gattgggtgg tctcaatgtt
     3481 gccattgga
//
LOCUS       DASSWZ010000140         6156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_910250, whole genome shotgun sequence.
ACCESSION   DASSWZ010000140 DASSWZ010000000
VERSION     DASSWZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910250"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1368)
                     /locus_tag="VFN58_03445"
     CDS             complement(46..1368)
                     /locus_tag="VFN58_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917078.1"
                     /translation="MIYANIAWLRRREARSPLANSGVNLRSAINAFLQLHAIEALGHR
                     EFRLLWLGHSFTSMAFWMDQVARGWLIYELTDSALQLGLVRGVQAIPTLLLSPLAGST
                     ADRYSRKTQILVAQVIDGLMLAALALLIVIGQIQPWHVYATAFGMAVVQTFQQPARAA
                     MIADLVPSSHLTNAIGLNSIIFNVARSTGPALAGVLIATFGTAGSYATQAVFYLLATI
                     WTAQLQPAERSSPGSRGHSVHGASLGRSIIEGWKFSWKNEAVRTSLLVVMLASLFIVP
                     FTTLLPVFARDILDVGATGQGLLLTAMGVGALGSAFIIAFFGDRMPRGIFMLGGVALY
                     GLSVVAFAASPWFQLSMVLMVIVGFANVCSHALVQTVIQTYSPSEFRGRTMAIFHMSN
                     VVMTIGSMLIGALAAFWGAPWASSSMALAGALAMVAIHVTLPGARHIR"
     gene            1872..3776
                     /locus_tag="VFN58_03450"
     CDS             1872..3776
                     /locus_tag="VFN58_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8917079.1"
                     /translation="MPTSCCGGDSPKAENEVDEESDGSCGRSFIERKSALANNLKKIL
                     QTIKGSSSTAKDARGEVNKTTIDGGTTRSCKSRVRAIGFSPGMYGKHFGLVELPFSVT
                     PDPRFSYVNSDYREAFATLRYGIETRKGFIVITGEAGTGKTTLLRRLMHTVEATVHTA
                     FIFNTHLSFTELLRLVLNELGVANSARDKQTLMAQLNDYLIEQLHKDHIVSLLIDEAQ
                     NLSDEMLEELRLLSNLETDRAKLIQIVLMGQPELERKLEQPKLRQLKQRVAVRCRLSP
                     LRSDEVAPYINSRLQTVGYEGKELFDLGSVQKIALYSKGIPRLINAICDNALLIAYAS
                     SQSKVSAKIVDEVGRDLQLVELSQVKTAERKEASRQTKAQPDDNLWRSEPEEFPAALQ
                     SPRRQADHKRGWAGVSIAILLGVIILAGTVLYSQQSGSLAALGVNIDDLVGGRLKDLV
                     RAQAKPASAPPIKDVVPEEERVTAEQPHIPTATLQGPELSAADSKQNDVASPKMENEA
                     ARPPTPLNEASTLKSESRTTPSDQTTGRTRKQTQQTQQTQQTQPFFYDAPVTAEKLEF
                     EVLKAIHNRAIRGVEVSVRDGTAYLTGQVATEKQKLAAAQAARSVPGVKEVRDQVIVS
                     SPMTARDTEG"
     gene            4041..5606
                     /locus_tag="VFN58_03455"
     CDS             4041..5606
                     /locus_tag="VFN58_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8917080.1"
                     /translation="MDSMYGKHFGLVELPFSVTPDPRFSYTNSLYREAFASLRYGIET
                     RKGFIVVTGEAGTGKTTLLRRLMRTVESTVHTAFIFNTHLEFTELLRLLLNELGVATS
                     ARDRLTLLAQLNDYLIEQLHKDHIVSLLVDEAQDLSDEMLEELRLLSNLETDRAKLIQ
                     IVLMGQPELERKLDQPELRQLKQRVAVRCRLAPLRSDEVAPYINSRLQTVGYRGRELF
                     DLGSVEKIALYSKGIPRLINVICDNALLIAYATSQSQISAKIVDEVARDLQLVERSQV
                     NATEEEKTTSRQASYDDERWRSEVGEFPAAFEQLPHQPGHKRGWAGAGIAIVLAVIIL
                     AGTVLYSQQSGSLAALGVNIDGLVPVHWKNPPQSQAPDNVEGAGVPLQVPHLQVAASR
                     PSEFLTSDPIGDGINGPARRDSSKFESSAGSTEIQSPKNDTPPPTSVDPNPQTDAKPK
                     PKERTYRVFDASFVRDKPRSDANITGTLEPGTRIKVQSKTGDYFRVRSLDRDREPISG
                     YVHREDAFFDPIK"
     gene            complement(5772..>6156)
                     /locus_tag="VFN58_03460"
     CDS             complement(5772..>6156)
                     /locus_tag="VFN58_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8917081.1"
                     /translation="TVDDILQARKFPDVIARSTYPVDIHNPEGSGTLLKRLPPGEAYD
                     IPLRCLLPRDVEDVLVAGRCISGTHEAHSSYRVMPVSMATGQAAGVCAALAARLGKSP
                     RAVPVSDVQNELTRQGANLGGKKNF"
BASE COUNT         1518 a         1685 c         1685 g         1268 t
ORIGIN      
        1 cataaatttg gaaccagtga tgggggttgt aggcacgcta accccctacc ggatatgccg
       61 cgcgccaggg agcgtcacgt ggatcgcaac catggccagg gcacctgcta gagccatcga
      121 cgacgacgcc cacggggcgc cccagaaagc cgccagcgcg ccgataagca tgctgcctat
      181 cgtcatcacc acgttgctca tatgaaagat ggccatagtt cgaccgcgga attcagaagg
      241 tgagtaggtc tgaatgactg tttgcacgag cgcgtgggaa cacacgttgg cgaaacctac
      301 gatcaccatc agcaccatgg atagctggaa ccaaggcgat gctgcgaacg ccacgacact
      361 gagtccgtat aacgccacac cccccaacat gaagatcccc ctaggcatcc gatcgccgaa
      421 gaacgcgatg atgaatgcgc tgccaagcgc gcccaccccc atagcagtca gaagcagccc
      481 ttgccccgta gcgccgacgt caagaatgtc ccgcgcaaat accggcaaaa gggttgtaaa
      541 aggcacgatg aatagagacg caagcatcac gacaagcagg ctggtgcgaa ccgcttcgtt
      601 cttccaactg aatttccatc cttcgatgat acttcgaccg agggaagcgc catgaacgga
      661 gtgacctcga gaaccgggag aagagcgctc tgcgggctga agctgcgcgg tccagattgt
      721 cgccaaaaga taaaacaccg cctgggtggc atacgagcca gcggtgccga acgtcgcgat
      781 gagcacgccg gccagcgccg ggccagtgct ccgcgccacg ttaaaaatga tcgagttgag
      841 gccgatggcg ttggtcaagt ggctggaggg cacgagatcg gcgatcatgg ccgcgcgggc
      901 cggctgctga aaggtttgga ccaccgccat gccaaatgcg gtcgcgtaca catgccaggg
      961 ttggatctgg ccgatgacaa taagcagagc caacgcggcc aacatcaagc cgtcgataac
     1021 ctgggcaacc aatatctggg tcttacgcga atagcgatcg gccgtgctcc ccgccagtgg
     1081 ggataacaaa agagtaggga tggcctgaac gccgcggacc aagcctagct ggagcgccga
     1141 atccgtcagc tcgtaaatca gccaaccgcg agcgacttga tccatccaga acgccatgga
     1201 tgtgaaagaa tgccccagcc agagcaaacg gaactcgcgg tggccgagcg cttcgatggc
     1261 atgaagttgt aggaaggcgt taatcgctga acgcagattt actccggagt tggccaatgg
     1321 cgagcgagct tcccgacgtc ttaaccaagc gatgtttgcg tatatcacgc aggaagaggc
     1381 agcgtccagt tactccggca cgttcgattg aaatttcaat cggcaggttc caagatcttc
     1441 acacgtaaat actcagcgac aattgaggtc tcactgatcc aatcgttctg accgtgccgg
     1501 gttattccac tcggcgcgtc cttccccgcg tgtaacgaag aattgcttgt gatcaattca
     1561 gctgtataac ttgatttccc gttatgagcc gtttggcttt gtcaccgaag gggtatccct
     1621 acaacgggtc cgtcagtttc cggctgtcca atcaaaattc ctggaatcta aacaagtttt
     1681 atccccgaga catctctggc acatgtcttg ctgccattct aattagtcaa cgtgtcgata
     1741 ttcggagttt tataatagtt gtctgacacg tccgctgaat cgctccaaat caaggatgat
     1801 tttcaagggt ccaactttgg cggcgccgct gcttcgcgga ctgttgccga ctggcgtcct
     1861 taatgtgaac gatgccaact tcgtgctgcg gcggagactc gccgaaggcg gaaaacgaag
     1921 tcgatgaaga atccgacggt tcgtgcggcc gatctttcat cgagcgcaaa agtgcactgg
     1981 caaacaatct gaaaaagatt ctacaaacaa ttaagggctc ttcttcgacc gcgaaagacg
     2041 cccgtggcga agtgaacaag actaccatag acggtgggac aactcgttct tgcaagtcgc
     2101 gggttcgcgc gataggtttc tctccaggta tgtacggcaa gcattttgga ttggtagagc
     2161 taccctttag cgtcactccc gatcctcgtt tttcgtatgt caattcagat tatcgggaag
     2221 ctttcgctac cctgcgctac ggtatcgaga cccgcaaggg cttcatcgtc attaccggcg
     2281 aggcgggaac cggcaagacc accttgctca gaaggctcat gcacactgtg gaggccaccg
     2341 tccatacggc ctttattttt aacactcatc ttagtttcac cgaattgctc cgattggtct
     2401 tgaacgagtt gggagtggcg aactccgcac gcgacaaaca gaccctgatg gcgcagctca
     2461 atgactattt gatcgagcag ctccataaag accacatcgt ctctctgctg atcgatgagg
     2521 ctcagaattt gagcgatgag atgctcgaag aactgaggct gctgtcgaat ctggagaccg
     2581 acagggccaa gctgatccaa attgtgttga tgggtcaacc ggagttggaa cgaaagctcg
     2641 agcagccgaa acttcgccag ctcaagcagc gggtggccgt tcgctgccgg ctgtctccct
     2701 tgaggagcga tgaggtggct ccgtacatta attctagact tcagactgtg ggctatgaag
     2761 gcaaagaatt attcgatctt ggatcagtcc agaaaatagc tctgtactcg aagggcatcc
     2821 ccaggctcat caatgcgatc tgtgacaatg ccctgctgat cgcctacgca agctcccaaa
     2881 gcaaggtttc ggcaaagatc gttgatgagg tggggcgcga cttgcaactc gtggagctgt
     2941 ctcaggtcaa aacggcagag cgaaaggaag catcccggca gacaaaagca caacccgatg
     3001 acaacctgtg gcgatctgaa cctgaagaat ttccagcggc cttgcaatcg ccacgacgcc
     3061 aggccgatca caagcgcggt tgggcgggcg tgagtatcgc aatcctgctg ggggtgatca
     3121 tactggctgg caccgtgctc tattcgcaac aaagcggttc gcttgccgct ttgggtgtca
     3181 atatcgatga tcttgtcggc gggcgcttga aagatctcgt ccgggcccaa gcgaagccag
     3241 cttcggcgcc gcccatcaaa gacgtagttc cggaggagga aagagttacg gctgaacagc
     3301 ctcatattcc aacggccacc ctgcaaggac cggagttatc ggcggcggat tcaaagcaga
     3361 acgatgtagc tagtccgaaa atggaaaacg aagcggccag gccgccaaca cctctcaacg
     3421 aagcaagtac tcttaaatcg gaaagccgca ccacaccttc cgatcaaacg acgggtagga
     3481 ctaggaaaca gactcaacag actcaacaga ctcaacagac tcagcccttc ttctatgatg
     3541 cgcctgtgac agcggaaaaa ctggaattcg aagtactcaa ggccatccac aaccgcgcga
     3601 taagaggcgt cgaagtctcc gttcgagacg gcacggctta tctcaccggc caagttgcga
     3661 cggaaaaaca aaaactagcg gctgcgcagg ccgcccggag tgttcccggt gtcaaggagg
     3721 tgcgcgatca ggtaatcgta agttctccta tgactgcgcg tgataccgag ggttgaaaaa
     3781 gaataggccg tgagggagac aaccaggcca ctttcacagc gcaaaaagat tcgaatcagc
     3841 tgaggagata tccggatttg acttatgcga accgaggcgg ctactggccc gggccaaggg
     3901 aaacggaagc tgctaggtgg actggagaaa attcttaata aactcttccg ttataggtgg
     3961 tcttgtgata ttgaataaaa cggttttctg tttgaaagca acacagcaag caaaactcta
     4021 acgcgaaacg ctccgataga atggacagta tgtacggcaa acattttgga ttggtagagc
     4081 tgccttttag cgtcactccc gatcctcggt tttcctatac caattctctt tatcgggagg
     4141 cattcgcttc cctgcgttac ggtatcgaga cccgcaaggg cttcatcgtc gttaccggcg
     4201 aggcgggcac cggaaagacg acactgctcc gaaggctcat gcgcaccgtg gagtccaccg
     4261 tccatacggc ttttattttt aacactcatc tcgaattcac tgagctgctc cgattgctct
     4321 tgaacgagtt gggagtagcg acctctgcac gcgaccggct gaccttgttg gcgcagctca
     4381 acgactattt gatcgagcag ctccataagg accacatcgt ctctctgcta gtcgatgagg
     4441 ctcaagatct aagcgatgag atgcttgaag aactgaggct gctgtccaat ctggaaaccg
     4501 acagggccaa gctgattcaa atcgtgttga tgggtcagcc cgagttggaa cggaagctcg
     4561 atcaaccaga actccgccaa ctcaagcagc gggtggcggt tcgttgccgg ttggctcctt
     4621 tgaggagcga tgaggtggcc ccctacatca attccagact gcagaccgtg ggctacagag
     4681 gcagagagct gtttgacctt gggtcggtcg agaaaatagc tctgtactcg aagggaatcc
     4741 cccgtctcat caatgtgatc tgtgacaatg ccctgctgat cgcttacgca acctcccaaa
     4801 gccagatctc ggcaaagatc gtcgatgaag tggcccgcga cctgcaactc gtagagcggt
     4861 ctcaggtcaa tgcgacagag gaagaaaaaa ccacttcccg tcaggcgagc tacgacgatg
     4921 aacggtggcg atctgaagtt ggggaatttc cggccgcctt tgaacagcta ccgcatcagc
     4981 ccggtcacaa gcgcggatgg gcgggggcgg gtatcgcaat cgtgctggca gtgatcatac
     5041 tggctggcac cgtgctctat tcgcaacaaa gcggctccct tgctgctttg ggtgtcaata
     5101 tcgatggcct ggttcccgtt cactggaaaa atccccccca gagtcaggcc ccggataatg
     5161 ttgaaggcgc aggtgttcca ttacaggtgc ctcatctgca agtggccgcg tctcgaccat
     5221 ctgagttctt gaccagcgat ccgattggtg acggcattaa tggtccggca agaagagatt
     5281 ccagcaaatt cgaatcctcg gcaggtagca cagagatcca aagcccaaaa aacgatacac
     5341 caccgccgac ctcagtagac cctaatcccc aaactgatgc taaacccaag ccgaaagaac
     5401 gaacttacag ggtcttcgat gcttcctttg ttcgagacaa accgcgctcc gacgctaaca
     5461 tcacgggcac gctagaaccc ggcactcgca tcaaggtgca gagtaagacg ggcgactatt
     5521 tccgcgttcg ctctttagac agagacagag agccgatcag cggttacgtt catcgcgagg
     5581 acgccttttt cgatcccatc aaatagcctt ctcatcgtcc cggatttcgc agctaatctt
     5641 acaacctgtg ctgcggaagg cttccgccct ggctcgtctt caaccgcatc atccagggtt
     5701 caaacgaaag agttggagaa tgtctgtgta aggacggtta cgataactag ggttattcgg
     5761 cttcgaaagt cttagaaatt ctttttaccg cctaggttgg caccctgtcg ggtgagctca
     5821 ttctggacgt ctgagacggg cacggcccgc ggagattttc ccaaccgcgc cgccagcgca
     5881 gcgcaaacac ctgcggcttg ccctgttgcc atggataccg gcatcacgcg gtaagacgaa
     5941 tgagcctcgt gcgtgccaga aatacatcgg ccggcaacta gaacgtcttc cacatcacgc
     6001 ggcaacaggc agcgcagcgg gatgtcgtaa gcctcgcccg gcggcaagcg cttaagcaag
     6061 gtgccgctgc cctcgggatt gtgaatgtct acggggtagg tgctgcgcgc gatcacgtcg
     6121 gggaattttc tcgcctgtaa aatgtcgtcc acggta
//
LOCUS       DASSWZ010000141         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_386700, whole genome shotgun sequence.
ACCESSION   DASSWZ010000141 DASSWZ010000000
VERSION     DASSWZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386700"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..796
                     /locus_tag="VFN58_03465"
     CDS             236..796
                     /locus_tag="VFN58_03465"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917082.1"
                     /translation="MDIDGVLADFVSPFLRVLEARAASGRIDPETYVDLSFSAHPELS
                     KEIVTECILKVSNDPGFWEQLNPLPTVEQWKVLDGLSRQEKLIFVTHRYECDNYSIHQ
                     VTRAWLRKHGVSNPVVYFTQNHKSELIGKLKVELFVDDRHENCRDVAENTDAVVLMPH
                     RPYNQTFKHPRVHRIQDLDELLPFIA"
     gene            824..1573
                     /locus_tag="VFN58_03470"
     CDS             824..1573
                     /locus_tag="VFN58_03470"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8917083.1"
                     /translation="MHELKDKVVIVTGCGGGIGGAIARHFAREGARIAVADINAESAQ
                     QRVVEITGQGGDAFTVVVDVTNKKSVHEMVRSTVERWGKIDILVNVAGGADPKLVIDM
                     EESDWDRIVNMNLKSVFLCSQAVLPTMIQQRYGKIVSISSIYGFTGCETRSNYAAAKA
                     GVAVFTKSLALEVAKDGINVNAIAPGRVATERVRGRYSDEEWAKRVAAIPLGRAATPE
                     EIAPTALFLVQDQNRYITGQTVHVNGAWLNW"
     gene            1586..2311
                     /locus_tag="VFN58_03475"
     CDS             1586..2311
                     /locus_tag="VFN58_03475"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917084.1"
                     /translation="MYGWRAKIGHVAPSRGDTLVYEFYKMLPEGFMLLNSTGTIRQLV
                     DADFERQLQRIEEAAADLVDNKCDAIIVGGSPLFTKLGYGSDIEMGKKLTAKYGVPIA
                     PGITGEVEALKSLNLKKLVVATPHEESLNQRMKSFLEASGFQVLKIEGYGVRKNADLT
                     DMDVHAAYKIAKRLYEQAPEADGIFIPCPRWPTITDVDLLEREIGKPVVTSCQSYIWH
                     GLRMAKVKEKITGFGRLMASLAN"
     gene            complement(2446..2769)
                     /gene="grxD"
                     /locus_tag="VFN58_03480"
     CDS             complement(2446..2769)
                     /gene="grxD"
                     /locus_tag="VFN58_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HET8917085.1"
                     /translation="MADEVLTQIDEKVKNNKVMLYMKGTPEFPQCGFSAHTVEILKSY
                     GVPFASEDVLADPAIRDGIKRYSKWPTIPQIYIGGKFVGGCDILHELHERGELEPMLK
                     SAFDK"
     gene            complement(2794..2913)
                     /locus_tag="VFN58_03485"
     CDS             complement(2794..2913)
                     /locus_tag="VFN58_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HET8917086.1"
                     /translation="MLEQHRMVNEALKEAMDDQRIHALALKTFSPAQWEKLGS"
BASE COUNT          704 a          781 c          786 g          650 t
ORIGIN      
        1 gcgatctcct tctcttcgcc cgttatactg acgatatcag ccacgtttcg ggcaagctca
       61 acaatccgtt gccgatccac ctgttcaagc acttgctgtt gcatcctctc tcctctcaac
      121 caattgtaaa atagtgctca aaggtttttg ttagcatatt cagttcagta ctgacaagaa
      181 cgaggttgac ggttgccgcc gatttgctta aatttgtacc tcgatgatga ttggattgga
      241 tattgacgga gtgctggcag atttcgtttc cccttttttg cgggtattgg aagcacgagc
      301 ggccagcgga cggatagacc cggaaaccta tgtggattta agtttttcgg ctcaccccga
      361 gttgtcgaag gagatcgtca cagaatgcat cctgaaagtt tccaacgatc ccgggttctg
      421 ggaacagctg aatccattgc cgacggtcga acaatggaag gttttggacg gcctgagccg
      481 ccaggagaaa ctaatcttcg ttacacaccg atacgagtgt gataactaca gtatccacca
      541 agtcacccgt gcttggctca ggaaacatgg cgtcagcaat cctgtcgttt atttcactca
      601 gaaccataag tctgagctaa tcgggaaact taaagtcgag ctgttcgtcg acgatcgcca
      661 cgagaactgc cgtgatgtgg ccgaaaacac cgacgctgtc gtgctcatgc cccaccggcc
      721 atataatcaa acgttcaagc acccgcgggt ccaccgaatt caggacctgg acgagttgct
      781 tccgtttatc gcgtaagtcc atcatcggtt tggtagagca atcatgcatg aactgaaaga
      841 caaggtcgtc atcgtcaccg gttgcggagg tggcatcggg ggcgccatcg cccggcattt
      901 cgcccgcgaa ggagcaagaa tcgcagtcgc cgacatcaac gcggagagcg cccagcagcg
      961 agtagtcgaa atcacgggtc aaggcggcga tgcctttacc gtcgtcgttg acgtaaccaa
     1021 caagaaatcc gtccatgaga tggtccgatc gacagtggag cgttggggaa aaatcgatat
     1081 tctcgtcaac gtcgcgggag gcgccgatcc caagttggtc atcgacatgg aagagtcgga
     1141 ttgggatcgt atcgtgaaca tgaacctgaa atcagtcttt ctctgctctc aggcggtgct
     1201 gccgaccatg atccaacagc ggtatggaaa aatcgtgagc atctcgtcca tctatggctt
     1261 caccggctgc gagacccgct cgaactacgc ggcagccaag gccggcgtcg cagtctttac
     1321 aaaatcgctg gcactagaag tcgcaaagga cggcatcaac gtaaacgcca tagccccggg
     1381 gcgggtcgca accgaacggg tgcgcgggcg ttattcggac gaagaatggg cgaaacgcgt
     1441 ggctgcgatt cctttaggtc gggccgcaac cccggaggag atcgcgccta cggccctttt
     1501 tctagtgcag gaccagaaca gatacatcac cggccaaacc gttcatgtga acggtgcatg
     1561 gctcaattgg taaggaggaa acccaatgta cggctggcgc gcaaagatcg gtcatgtggc
     1621 tccgtcccgc ggcgacaccc tggtgtatga gttttacaag atgcttcccg aaggattcat
     1681 gctcctcaac agcaccggaa ccatcaggca actggtggac gcggatttcg agagacagct
     1741 gcaaagaatc gaggaagccg cagccgattt ggtcgataac aaatgcgacg cgatcattgt
     1801 cggcggatcg ccgctgttca cgaagctcgg ctatggcagc gacatagaga tgggaaaaaa
     1861 gttgacggcg aaatacgggg taccgatcgc accggggatt actggcgagg ttgaggcgtt
     1921 gaaatcactc aaccttaaga agctcgtggt ggccactccg cacgaagagt cactcaatca
     1981 aagaatgaaa agttttcttg aggcttccgg cttccaggta ctgaaaattg aaggatacgg
     2041 cgtacgaaaa aatgccgacc tgacggacat ggatgtccat gccgcctaca agatcgccaa
     2101 gcgactctac gagcaagcac ccgaggccga cggcattttt atcccatgtc ctcgctggcc
     2161 gactatcacc gatgtagatc tgctggagag ggaaatcggc aagccggtcg taaccagctg
     2221 tcaatcttac atctggcacg ggttgcgaat ggccaaggtc aaggagaaga tcaccggctt
     2281 tggccggctg atggcgtctc tggcgaactg aagccttgcg gcaacagacg gtagccctat
     2341 ttgctcctac caatacttcc ttcaaaagat cgttcacgcg cgctcaggcc caaggctgag
     2401 acgctgctca atcttcgggg aaaaccatcc cgctggactg aagagttact tgtcgaaagc
     2461 ggacttcagc atcggctcga gttcgccgcg ctcgtgaagc tcatgaagaa tgtcgcagcc
     2521 gccgacgaat ttcccgccga tgtagatctg tgggatggtc ggccacttcg agtagcgctt
     2581 gataccgtct ctaattgcgg gatccgccag aacgtcttca cttgcgaacg ggacaccgta
     2641 ggatttcagt atctcgactg tgtgagctga aaaaccgcac tgcggaaact ccggtgtgcc
     2701 cttcatgtag agcatcacct tattgttctt cactttttca tcgatctgtg tcaaaacttc
     2761 atcggccatt ttagttctcc ttcttactaa gctttaggaa ccaagcttct cccactgcgc
     2821 gggtgaaaaa gtcttcagcg ccaatgcatg gatacgttgg tcgtccatcg cctctttgag
     2881 agcctcgttg accatccggt gctgttcgag caacccctta c
//
LOCUS       DASSWZ010000142         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_499828, whole genome shotgun sequence.
ACCESSION   DASSWZ010000142 DASSWZ010000000
VERSION     DASSWZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499828"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFN58_03490"
     CDS             complement(<1..232)
                     /locus_tag="VFN58_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HET8917087.1"
                     /translation="MKFEREQGYFVGAIYINYTATVAIAVPGFFILDTFTRMTINQQL
                     AIWVPFSVIFPLLFFHHSRSLWLVLDHLFNPAK"
     gene            complement(354..5339)
                     /gene="uvrA"
                     /locus_tag="VFN58_03495"
     CDS             complement(354..5339)
                     /gene="uvrA"
                     /locus_tag="VFN58_03495"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET8917088.1"
                     /translation="MNLFLSQENWRREPFSNHLHCPHCDISYRDPVANLFSFNSPLGA
                     CDMCRGFGRVIDIDLDLVIPDPGKSLSEGAIKPWINRTRRTRRLLDFCDRKRIPTKKP
                     FGELTEKQRQLIMDGDGEYKGIRGWFRRLERKSYRMHVRVLLSRYRAYLLCPDCQGSR
                     LKPDALHYRIEGKDIAQVNAMNVGEAHRFFTQLKPAGAKDQVASLILDEIRRRLSYLV
                     GVGLEYLTLDRQSRTLSGGELERVDLTTAIGSSLVNTLYVLDEPSIGLHPRDSRRLVE
                     ILHRLRANRNTVVVVEHDPEIIKESDYIIDLGPKAGEQGGQLMFAGPYSDLLKSEESL
                     TAAYLSQRKMIPFPARNRKQLLQRSIKITGAQANNLKNIDVEIPLGVFVCITGVSGSG
                     KSSLVDEVLHRNLKKLKEAPTANIVHCAKIEGVDRISEVVLVDQSPVGTTPRSNPVTY
                     LKVFDAIRRLFATADLSRMRGYTPSTFSFNVEGGRCETCRGEGFEKIEMQFLSDVYTS
                     CPECHGSRYREEILEVTYRGKNIRQILDLTVSEAIEFFKDLSEIKEGLYPLRAVGLEY
                     VRLGQPLTTLSGGESQRLKLAAHMAKAKKAGTLFIFDEPTTGLHFHDIERLLWAFNEL
                     IDQGHSVVVIEHNLEVIKCADHIIDLGPEGGDGGGCIVATGTPEQISRVESSYTGVYL
                     RPYLKTAAVSPFTPQLEKAAKVQEFKSSTEENAITIVGAKEHNLKNISLNIPRDRFVV
                     ITGLSGSGKSSLAFDIIYAEGQRRYIDSLSAYARQFLQIMAKPNVDLLAGIPPTVAIE
                     QRLSQGGKKSTVATVTEIYHYLRLLYSKVGKQHCVQCGRQIRSLSRSQIFDRIGRSYR
                     GKEVMILSPIVRGRKGFHKEVIAGARRLGYRRARIDGELLDLRAPQLTQGLERFKEHD
                     IDIVIGKAKAGGREVEALVDQGLRIGNGVIHLISERGEQIFNQRLFCLRCGIGYEPLD
                     PRLFSFNSRQGACSECGGMGFQWDFDPELLFANPQQSVSETLNGISAFTPENGTSLSR
                     SMDRLVAELAKKHNVDIDKPFGRLAKKTQDQVLYGGNGRSGFVGFIPYLRELSQHGEE
                     NGNQELSELLSESRCKSCHGRRLNSRAQAVRVDGKAIWQLTALSVQEAKDYFDSLQIA
                     YADNGAAERDRAVADKILKEIQQRLTFLSEVGLPYLTLDRRADTLSGGEAQRIRLAAQ
                     LGSNLRGVCYILDEPTIGLHPRDNAMLLRTLRRLEAVGNSVLVVEHDEATIESADLIV
                     DLGPGAGVRGGNVVSIGSPTEIRNNPNSLTGVFLKAERKRLGPTRELGKTNWLTIREA
                     KANNLKNVDVRLPLGIWTCVTGISGSGKSTLVKEILYKGLKMKIGQFAGRPGAHREII
                     GWKSLERIVEVDQTPIGKTPRSVPASYVGLLDEIRKLYALAPESRLRGYTSSRFSFNV
                     RGGRCEECAGQGKIRKEMSFLPDVFVDCEVCGGERFNEETLSIRYNDKNIADVFHMTV
                     EEAVPFFHAIPKVARPLKILDDIGMGYITLGQASNTLSGGEAQRIKLAYELGKESHGK
                     TLYVLDEPTTGLHFADVEKLIHILHRLVEMGNTVVTIEHNLDIVKDADYLIDLGPEGG
                     EQGGYIVACGPPLEVANNSKQSYTARFLREYLNGGPANSFSNVTAAHQEVS"
BASE COUNT         1153 a         1483 c         1508 g         1198 t
ORIGIN      
        1 tcttggctgg gttgaacaga tgatcgagca ccagccagag gctcctcgaa tggtggaaaa
       61 acaacagcgg gaagatcacc gagaacggga cccagatcgc cagctgttgg ttaatcgtca
      121 tgcgggtgaa ggtatccaaa atgaaaaagc cgggaactgc gatcgccaca gtcgctgtat
      181 aattaatata aatcgcgcct acgaaatagc cctgctctcg ttcgaacttc aagccgcatt
      241 gaaaacaatg actgttcatg gagaaagggc gccgatacaa gggacccacg caacagcgcg
      301 ggcacttgag ccgaaggccg gccctgagca tcgatagaat cttattcggc atgctacgat
      361 acttcttgat gcgctgctgt aacgttgctg aatgaatttg ctggcccgcc attgaggtac
      421 tcccggagga atcgagccgt ataggattgc ttgctattgt tggcaacttc caagggtgga
      481 ccgcaggcga cgatgtagcc gccttgttcg ccaccttcag gtcccagatc gattagataa
      541 tccgcatctt ttacaatgtc gaggttgtgc tcaatggtga cgaccgtgtt gcccatctcc
      601 accaggcgat gaagaatgtg aataagtttc tccacgtcgg caaaatggag ccccgtggtc
      661 ggctcatcca aaacgtagag cgttttccca tgggactcct tgcccagctc ataggcgagc
      721 ttgattctct gcgcttcacc cccggacagg gtgttgctgg cctgcccgag tgtgatgtag
      781 cccatgccga tgtcatcgag gatcttgagc ggccgggcga cttttggaat ggcgtggaag
      841 aaaggcacgg cctcttctac ggtcatgtgg aaaacgtcgg cgatattttt atcgttgtaa
      901 cgaatgctga gcgtctcttc attaaaacgc tcgccgccgc agacctcaca atccacgaat
      961 acatccggca aaaaactcat ctctttgcga atctttccct gtcccgcgca ttcctcacag
     1021 cgcccgccgc ggacattaaa cgaaaatcgg ctcgaggtat agccccgtaa ccgagactcc
     1081 ggcgccagcg cataaagttt gcgaatttcg tccagcaacc cgacatacga ggccgggaca
     1141 gagcggggag ttttgccaat gggggtttga tcgacctcga ctattcgttc cagcgacttc
     1201 cagccgataa tctccctgtg cgcacccggc cgtccggcga attggccgat tttcatcttc
     1261 aggcccttgt agaggatttc cttgaccaaa gtgctttttc ctgacccgga gatccccgtg
     1321 acacaggtcc atatgccgag gggcaagcgc acgtcgacgt ttttaagatt gttagctttt
     1381 gcttctcgga tggtgagcca attagttttg ccgagttcac gcgtcgggcc gagccgtttc
     1441 cgctcggctt tgagaaacac gccggtaaga gaattggggt tgttcctgat ctccgtgggc
     1501 gatccgatgg aaacgacgtt cccgccacga acgcctgcgc ccggtccgag gtcgacgatg
     1561 agatcggcgg attcaatagt tgcctcgtcg tgctcgacta ccaacacgct gttaccgact
     1621 gcctcgagcc ggcgcagggt gcgcagtagc atggcgttgt cgcgcgggtg aagaccgatc
     1681 gtaggttcgt ccaggatgta acagacgccc cgcaggttag agcctagctg ggccgccagg
     1741 cgaatgcgct gagcttcccc gccagaaaga gtatccgccc ggcggtcgag ggtcagataa
     1801 ggcaaaccga cttccgagag gaatgtaagt cgttgttgaa tttctttcag aatcttgtcc
     1861 gccaccgcgc ggtcgcgttc ggcagcgccg ttatcagcgt aggcgatctg tagtgagtcg
     1921 aaatagtctt tggcctcctg taccgacagc gccgtgagtt gccaaatcgc cttgccgtcc
     1981 acgcgcacgg cttgagctcg tgagttgagt cttcgcccat ggcaactctt gcagcgagat
     2041 tcgctcaaaa gctctgagag ctcttggttt ccattctctt cgccatgctg cgagagctcc
     2101 cgcagatagg ggatgaaacc gacaaatccg gagcggccat taccaccata gagcacttgg
     2161 tcctgggttt tcttggccag gcggccgaac ggcttatcaa tatcgacgtt gtgctttttt
     2221 gccaattctg ccacaagccg gtccattgag cggctcagac tggttccatt ttcaggggtg
     2281 aaagccgaaa taccgtttaa agtctcgctg accgactgct gcggattggc gaagagcagt
     2341 tcaggatcga agtcccattg aaagcccatg ccgccgcatt ctgaacaagc cccttgccgg
     2401 ctgttgaacg agaagaggcg cgggtccaag ggctcatagc cgatgccgca tcggagacaa
     2461 aaaagacgct gattgaagat ttgctcgccc cgctccgaga tgagatgaat cacgccattg
     2521 ccaatccgga gcccctgatc caccagcgct tcaacctcgc gtccgcccgc tttcgccttg
     2581 ccgatcacaa tgtcgatgtc atgctcctta aaccgctcca agccctgggt gagctgaggc
     2641 gccctgaggt cgagcaactc gccgtcgata cgtgccctgc gataacccaa ccgtcgcgcg
     2701 ccggcgatca cttccttatg gaaacctttg cgcccgcgca cgatggggct caatatcatc
     2761 acctctttgc cgcgatagga acgaccgatg cgatcgaaga tttggctgcg actgagggac
     2821 cgaatttgcc ggccgcactg gacacaatgc tgttttccga ctttagaata gagcagccgc
     2881 aggtagtgat agatctccgt caccgtggca acggtagatt tcttgccacc ctggctgagc
     2941 cgctgctcga tggcgaccgt cggcggtatg ccggcaagca gatcgacatt aggcttcgcc
     3001 atgatctgaa gaaactgccg cgcgtaagcg gaaaggctgt cgatgtaacg ccgctgtcct
     3061 tccgcgtaga tgatgtcgaa ggccagtgag gattttcccg agccgctcaa gccggtgatg
     3121 accacgaaac ggtctcgggg tatgttgagg ctgatatttt taagattgtg ctctttggcg
     3181 ccaacgatcg taatggcgtt ctcttcggtt gaactcttga actcttgaac ctttgcggct
     3241 ttctcaagct ggggcgtgaa aggggatacg gctgctgttt tcagataggg ccgtagatag
     3301 acgccggtgt aagagctttc gacgcgtgaa atttgctccg gagtacccgt cgcgacgatg
     3361 cagcctccac cgtcgccacc ctccggccct aggtcgatga tgtgatcggc gcacttgatc
     3421 acttccaggt tgtgctcgat gacgacgacc gagtgaccct gatcgatcaa ttcattgaac
     3481 gcccaaagca ggcgctcgat atcgtggaaa tgtaaaccgg tggtaggctc atcgaaaatg
     3541 aaaagcgttc cggccttctt ggccttggcc atgtgtgcag ccaattttaa ccgctgcgac
     3601 tctccgccgg agagagtcgt gaggggttgt ccgagccgaa cgtattccag tccgacggcg
     3661 cgcagtggat acagcccttc ttttatttca gacaggtcct tgaagaactc tatagcctcg
     3721 gagaccgtaa gatccaaaat ctgtcgaatg tttttgcccc ggtaggtgac ttcgagtatc
     3781 tcttcacggt accggctgcc gtgacactca ggacaagagg tgtagacatc cgagaggaac
     3841 tgcatctcga ttttttcaaa accctcgccg cggcaggttt cgcagcgtcc gccttccaca
     3901 ttgaaagaaa aggtcgacgg cgtgtacccg cgcatgcgcg agaggtcggc ggtggcaaag
     3961 agacggcgaa tggcgtcgaa cactttcaaa taggttaccg gattagacct cggcgtggtt
     4021 cccaccggag actgatcgac caggacgacc tcggagatcc tgtcgacccc ttcaattttc
     4081 gcgcagtgga cgatattagc ggttggagct tccttgagct tcttcaggtt gcggtgaaga
     4141 acttcatcga cgaggctcga tttccccgat cctgagacac ccgtaatgca gacaaagaca
     4201 cccaggggaa tctcgacgtc gatattcttc aggttgttcg cctgggcgcc ggtgatcttg
     4261 atcgaccgtt gcaaaagctg cttacggttg cgtgcaggga atgggatcat cttgcgttgg
     4321 ctcaggtaag ccgccgttag cgattcttcg ctcttcagaa gatcactata tggtccggcg
     4381 aacatcagtt gcccgccctg ttcgccggct ttggggccga ggtcgatgat gtagtcgctc
     4441 tctttgatga tctcggggtc gtgctccacc acgacgacgg tatttcgatt cgcgcgcagg
     4501 cgatggagga tctccaccag ccgacggcta tcgcgcggat ggaggccgat ggagggctcg
     4561 tccagcacat acaacgtgtt gactaacgat gaaccgatgg cggttgtcag gtcgactcgc
     4621 tccaactcac cgccggagag cgtccgcgac tggcggtcca acgtcaagta ttcaaggccg
     4681 acgccgacca aataactgag tcgccgccgg atctcgtcga gaatcaggct cgcgacttga
     4741 tctttagcgc cggcgggttt gagttgggtg aagaagcggt gagcttcacc gacattcatg
     4801 gcattgactt gcgcgatgtc cttgccttcg atgcgatagt gaagcgcgtc cggtttgaga
     4861 cggctgccct gacagtcggg acagaggaga taggcgcggt agcgggacaa cagcactcgc
     4921 acatgcatgc gataactctt gcgctccaag cggcgaaacc agccccggat tcccttgtac
     4981 tcgccgtcgc catccatgat cagctgcctt tgcttttcgg tgagttcccc gaacggcttc
     5041 ttggtgggga tccgtttgcg atcgcagaaa tccaacagcc gccgagttct acgggtgcgg
     5101 ttgatccatg gcttgatggc gccttcgctc agggacttgc cgggatccgg aatgactaga
     5161 tccaaatcaa tgtcgatgac ccggccaaac ccacggcaca tatcgcaagc tcccaacggg
     5221 ctgttaaacg agaagagatt agccacggga tcacgataag agatgtcaca gtgcgggcaa
     5281 tggagatgat tgctgaaggg ttcccgccgc caattctctt gcgacaagaa aagattcaac
     5341 cg
//
LOCUS       DASSWZ010000143         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1122488, whole genome shotgun sequence.
ACCESSION   DASSWZ010000143 DASSWZ010000000
VERSION     DASSWZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122488"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFN58_03500"
     CDS             complement(<1..421)
                     /locus_tag="VFN58_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917089.1"
                     /translation="MQRLEITIKFFLALASTVFLLISGVVLPAAGVILIPFVPQPVLL
                     FGLKYGTGLGVAVLVAALVSLFVLAGEALGFVYSIFAVMVGLLFWLLGRVRAVELLVT
                     GVAAAMLAVAAGVLLHFYGSWGAMIQDFRAGLIDNLTV"
     gene            complement(445..723)
                     /gene="rpsR"
                     /locus_tag="VFN58_03505"
     CDS             complement(445..723)
                     /gene="rpsR"
                     /locus_tag="VFN58_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET8917090.1"
                     /translation="MGARPYRERTDDEKGAPRRRPMFRRKVCRFCADKTLRIDYKDVR
                     TISQFVTERGKMTPSRITGNCARHQRLLTTAIKRARSVALLPFTTANS"
     gene            complement(789..1181)
                     /gene="rpsF"
                     /locus_tag="VFN58_03510"
     CDS             complement(789..1181)
                     /gene="rpsF"
                     /locus_tag="VFN58_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET8917091.1"
                     /translation="MGGIRTVALYETLFVVHPEKGTRMKEFIERFRKVVEGQEGTVAH
                     VDEWGLRDLAYRIDKQGKGFYTLFQYNSSARAVEELERNLRLTDGILRYLTVRAEEEI
                     QNQPAAKNHSEEHRAPAEGAAAKSEPRT"
     gene            complement(1234..1800)
                     /gene="pth"
                     /locus_tag="VFN58_03515"
     CDS             complement(1234..1800)
                     /gene="pth"
                     /locus_tag="VFN58_03515"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET8917092.1"
                     /translation="MKLVVGLGNPGRKHERTRHNLGFLIIDQIARQNRVTVKKKLCEA
                     MVGEWSNDGERVLLVKPQTYMNRSGESVKCLLGHFRSAPEDLIVIYDDLDLAFGRIRI
                     RPTGGAGGHRGVLSILESLPGAQFYRVRVGIGRPPDVMDPVDFVLEHFTLQEIEQLDE
                     IVSRAAEAVVSLLREGGQRAMEQYNRAH"
     gene            complement(1836..2492)
                     /locus_tag="VFN58_03520"
     CDS             complement(1836..2492)
                     /locus_tag="VFN58_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET8917093.1"
                     /translation="METLEIQAEAREVASKREARRLRRAGKIPGVLYGPKTPPVPLEL
                     NKREFSSRVAGLEGSHLVRLKSGSSALAAKVALVKDMQYHPITGDVIHTDLYEVDLTA
                     KIQVHVPLHFMGKAVGVVRGGILQPIVREVEVECLPMDIPEFFNVEVSALDIGDAVHI
                     EELQMPEGVTAIFETNFALVTVVPPSVEEAPTPTPAVTEGVVATPEVQEEPKEGGEES
                     "
     gene            complement(2526..3479)
                     /locus_tag="VFN58_03525"
     CDS             complement(2526..3479)
                     /locus_tag="VFN58_03525"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET8917094.1"
                     /translation="MSQHAVELKIFAGNSNPPLAEEISQYLKVPLGKAVVETFSDGES
                     KVEIKENVRGTDVFVLQSTSAPGNNNLMELLLMLDAFKRASAKRITAVVPYYGYARQD
                     RKVIPRVPISAKLVANLITTAGASRMLTLDLHSGQIQGFFDIPVDNLYATPVLLQHLK
                     KQLNHNEVTIVSPDAGGVERARAIATRLDASLAIIDKRRVAPNVVAEMNIIGEVKDQV
                     AILLDDMVDTAGTLTLAADALKQEGAKKILGCCTHAVLSGPAIQRIEESPLEELIVTN
                     TVPLAADAQRCRKIKVLSVAHLIGEAIRRTHDEESISSLFV"
     gene            complement(3640..3713)
                     /locus_tag="VFN58_03530"
     tRNA            complement(3640..3713)
                     /locus_tag="VFN58_03530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3679..3681),aa:Gln,seq:ttg)
BASE COUNT          871 a         1111 c          907 g          879 t
ORIGIN      
        1 cgaccgtaag gttatcgatg aggccggcac gaaaatcttg aatcatcgct ccccaggaac
       61 cgtagaaatg aagcaataca cctgcggcta ccgcaagcat cgcagccgcg actcccgtga
      121 ctagtaattc aacggcgcga acccgtccca atagccaaaa aagcagaccg accattacgg
      181 cgaatatgga ataaacaaaa cccaatgctt cccccgcaag cacaaacaag ctaaccagcg
      241 cggcgacaag gacggccacc cccaagcccg taccgtactt gagaccaaat aaaagtaccg
      301 gctggggaac gaacgggatc aagataacac cggctgcagg caataccaca ccgctaatga
      361 ggagaaaaac ggtagaggcc aaggccagaa agaacttgat cgtgatttct agccgctgca
      421 tattaaaacg gttggcgaag ataactaact gttggcagtt gtaaacggca gtagggcaac
      481 gctgcgggcc cttttgatcg ccgtcgttag caacctttga tgacgagcgc agttgccggt
      541 aattcgactc ggggtcattt ttccgcgctc agtgacgaac tgcgagatcg tcctcacgtc
      601 cttgtaatcg atcctcaggg ttttgtcggc gcagaaacga cacactttac gccggaacat
      661 cggtctccga cgaggcgccc ccttttcgtc gtccgttctc tcccgatatg gcctcgcgcc
      721 catctctctc tgtgccatag atttgcctac ctcactatta acggttgtac ccgctcttcg
      781 agaagttact aagtccgggg ttcagatttt gcggctgctc cctcggctgg tgctcggtgc
      841 tcttctgagt ggttctttgc agcgggttgg ttctggattt cttcttcagc acggacggta
      901 aggtagcgta agataccatc agtgagccgc aggttgcgtt ccaactcctc gaccgctcgg
      961 gccgacgagt tgtattggaa tagcgtgtaa aacccctttc cctgcttgtc aatccgatac
     1021 gccagatcac gcaagcccca ttcatcgaca tgagcgaccg tgccttcctg cccttcgacg
     1081 actttcctga acctctcaat aaattctttc attctcgttc ccttttcggg atgcaccacg
     1141 aaaagagttt cataaagtgc cacagttctt atacccccca cgacaatttt tgttctgtat
     1201 cataaacata gccccctggc gtttacaacg agcttagtgc gcacggttat actgctccat
     1261 ggcccgttgt ccaccctccc gcagcaaaga taccaccgct tcagcggccc gtgaaactat
     1321 ctcgtcgagt tgctcaatct cttgcaaggt aaagtgttcc aacacaaaat caactgggtc
     1381 cataacgtcg ggcggccgcc cgattcctac acggactcta taaaactggg ctcccggtag
     1441 gctttccaga atcgagagga cgccccgatg gccacccgct ccccccgtgg gccgaatccg
     1501 tatacgccca aatgccaagt cgagatcgtc atagatcaca atcaaatctt caggtgctga
     1561 tcggaaatgg ccaaggagac atttaaccga ttcgccacta cggttcatgt aggtctgggg
     1621 cttgaccagc agaacccttt ctccatcgtt cgaccactcg ccgaccatgg cctcgcagag
     1681 cttttttttg accgtaaccc gattctgccg tgcaatctgg tcgatgatca aaaaccctaa
     1741 gttatgccgc gtccgttcgt gcttcctgcc gggattcccg agacccacga cgagtttcac
     1801 caatcgattc ctgaagatcc tgatgaggca gctgcttagg actcctcgcc accttccttt
     1861 ggctcttcct gaacctctgg cgtggctaca actccctccg ttactgccgg ggtcggagtc
     1921 ggagcctctt caaccgacgg cggtaccacg gtcaccaagg caaaattcgt ttcaaagatg
     1981 gcggttacac cctcgggcat ctgcagttct tcgatgtgaa ctgcatcccc aatatcgagc
     2041 gcactgactt cgacattgaa aaattctggt atgtccatgg gtaaacattc aacctcgacc
     2101 tcgcgcacga tcggttgcag gatgccaccg cgtacaacgc ccaccgcttt gcccatgaag
     2161 tgaaggggca catgcacctg gatcttcgcc gtaaggtcaa cttcgtaaag atccgtgtga
     2221 ataacatcgc cggtaattgg atggtattgc atgtctttaa ccaaagccac tttggccgcc
     2281 aaggcagaag agccggactt cagccggacc aaatgcgagc cttcgagtcc agccacacgg
     2341 ctcgagaatt ctctcttgtt aagttccaaa ggaaccggcg gagttttggg gccatacaga
     2401 acaccgggaa tctttccggc acggcgcaac cggcgcgcct ctcgtttgct ggctacttcc
     2461 cgcgcctcgg cttgaatttc tagcgtttcc aacttacctc ctattgtatc gttactcgga
     2521 tccgttcaga cgaacaaaga actaatggac tcttcgtcgt gggtgcgccg aatcgcctcg
     2581 ccgatgagat gagccacgga taagactttg atcttgcgac agcgttgcgc atcggccgcc
     2641 aaaggcaccg tattcgtaac aatcaattcc tccagtggcg attcctcgat tcgctgaatc
     2701 gcgggtccgg aaagcacggc gtgagtacag cagccgagaa tttttttggc accttcctgc
     2761 ttcagtgcgt cggcagccaa cgtgagggtg ccggcagtat cgaccatgtc atcgagtagg
     2821 atcgcgacct gatctttaac ttcaccgata atgttcatct ccgccactac attaggagcg
     2881 acccgccgct tatcaatgat cgccagtgag gcatccaatc gtgtcgcaat ggcccgcgct
     2941 ctctctaccc cgccggcatc cggtgagacg atggtgactt cattatggtt cagttgtttc
     3001 ttcaaatgct gcagtaggac cggcgttgca tacaagttat ccacggggat atcaaaaaaa
     3061 ccttgaattt gtcccgaatg cagatcgagg gtcagcattc gagaagctcc cgcggtggtg
     3121 attaagttag cgaccagttt tgcgctgatc ggcacgcgcg gaataacctt ccggtcctga
     3181 cgcgcatacc cataataagg gacgaccgcg gttatgcgct tcgctgaggc tcgtttgaag
     3241 gcgtccaaca tgagcaaaag ctccatcaag ttgttgtttc ccggagcgga cgtcgattgc
     3301 agaacaaaca cgtctgtgcc acggacattt tccttgatct cgaccttact ctcaccatca
     3361 ctgaaagtct ccacgaccgc ttttcccaac ggcaccttaa ggtactgact gatctcctca
     3421 gccaaaggag gattcgaatt tccagcgaag atctttagct cgacagcgtg ttggctcatg
     3481 gtctcgaaac tattctttcg gcacagttct cacaaccatc cgtgttggcc cgtgatccga
     3541 ccaggcgtct actgaaatga gtggcgccgt tcgcttatga acggcgccac ttaaatccta
     3601 gcttgattgt acttttctta tcgactttgg ctaagaaact ggctgggcgg gaaggattcg
     3661 aaccttcgat accggatcca aagtccgggg ccttaccact tggcgaccgc ccaccccgaa
     3721 ctcctacaag agcagctcaa ccgcccggac actatctctg ctcatccg
//
LOCUS       DASSWZ010000144         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1179337, whole genome shotgun sequence.
ACCESSION   DASSWZ010000144 DASSWZ010000000
VERSION     DASSWZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179337"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFN58_03535"
     CDS             <1..256
                     /locus_tag="VFN58_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8917095.1"
                     /translation="GPFDLCFIDADKESYGDYYDRAVDLVRQGGLIVLDNMLRGGRVL
                     SPRDQDARTVAALNNRIQKDQRVENVLLPIRDGVMLAYRR"
     gene            352..585
                     /locus_tag="VFN58_03540"
     CDS             352..585
                     /locus_tag="VFN58_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET8917096.1"
                     /translation="MGILIDYLKLIGWGAVGIVTMAFSLWILLSLFTWLTPVDEWDEL
                     KKGNLAIAIVMAAVIIGFALVISAAIAPPPVAS"
     gene            703..1305
                     /locus_tag="VFN58_03545"
     CDS             703..1305
                     /locus_tag="VFN58_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET8917097.1"
                     /translation="MAYKLEGSLLEVCNCEVLCPCWIGVDPDRGTCDSALAHHFEKGE
                     IDGVDVSGLTLGFIVHIPGNVLKGNWRAVVHIDQRATKQQEEALLSVYTGKQGGPIAD
                     LVQLIGEVVAVERSPITFEVKEGKGTLRIGTIADCEMEPFRGPSGEVTTLHDSIFSTI
                     PGSPAYVAKATKFRMKNPALGQNVDLNDHNAIQGNFVFQS"
     gene            1494..2219
                     /locus_tag="VFN58_03550"
     CDS             1494..2219
                     /locus_tag="VFN58_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET8917098.1"
                     /translation="MGSLVASAWLALWIWDRSPYSRYLGHEQLGEIGAASLLLPITLY
                     LVGWTLMTVAMMLPTTLPLLDIFRRLTVRRQERSQLMALVIAGYLGVWMGFGVAAHVF
                     DWGLHQIVERISWLETNAWVVGAGTLLLAGSFQFSRLKYRCLDKCRAPLSFVMQYWRG
                     RHDRRNALLLGIHHGIFCVGCCWALMLLMFAVGVGNVAWMLVLGAVMAVEKNMPWGRK
                     LSAPLGVVLLAWGGFILLNHFRL"
BASE COUNT          504 a          582 c          708 g          613 t
ORIGIN      
        1 ggggccattt gatctttgtt ttatcgacgc ggataaagaa agttacggcg attattacga
       61 ccgcgccgtc gatctggtgc ggcagggcgg gctgattgtc ctggacaaca tgcttcgggg
      121 cgggagggtt ctcagtccga gggatcagga cgcgcggacg gttgcggccc tgaataatcg
      181 gattcaaaaa gaccagaggg tcgaaaacgt tctgcttccc attcgcgacg gcgtcatgct
      241 ggcctatcgg cgctgattcc ggaccagtgc gttgactccg tttggaaata ccttgacgcc
      301 gggccgcata ccaaataggc tgcggcccaa tggtaaaagg agaacggacc gatgggtatt
      361 cttatcgatt atctgaaact aataggctgg ggcgctgtcg gcattgtcac aatggctttt
      421 tccctctgga tcttgctgtc gctcttcacc tggctgacgc cggtggatga gtgggatgaa
      481 ttaaagaagg gaaaccttgc gatcgctatc gttatggccg ctgtgatcat cggatttgct
      541 ctggtgatct ccgcggcaat cgcgccacca ccggttgctt cgtgagtcgc ggcgatccga
      601 tcaatagggc cggctcatcg ttctacgttt cggatcgaca ttttttctca tctttgataa
      661 ctgagttgga gtacgtcctg cagccttaaa aagggaggat atatggctta caaactcgaa
      721 ggcagtcttc tcgaagtgtg taattgcgaa gtgctgtgtc cttgttggat cggcgtggat
      781 ccggacaggg gaacttgcga ttctgccttg gcgcatcatt tcgaaaaagg ggaaatcgac
      841 ggtgtcgatg tatcgggtct caccctggga ttcatcgtac acattcccgg aaatgtgctg
      901 aagggtaact ggcgtgccgt cgtccacata gaccaaaggg ccacaaagca acaggaagaa
      961 gcgctcctta gcgtctatac cggcaaacag gggggtccga tcgcagatct cgtgcagttg
     1021 attggcgagg tggttgccgt cgagcggtcg cccatcactt tcgaagttaa ggagggaaag
     1081 gggacactgc ggatcggaac catcgccgat tgtgagatgg aacctttccg tggtcccagc
     1141 ggcgaggtga caacgctcca cgattcgata ttttcgacga ttcccggctc accggcatac
     1201 gtggccaagg cgacgaaatt cagaatgaaa aatcctgcct tggggcaaaa cgttgaccta
     1261 aacgaccata acgcaattca agggaatttc gttttccaga gttaagctat tgaatcacat
     1321 tacgcgcagg gcaatggagt tagataaaaa ccctcttact cagcacgcgg cggtcgactg
     1381 gattagtgaa gtgatggagt aatcgagtca tgcaatcaaa aaccaacact ccaatactcc
     1441 catacttcaa cactccattt acctcatccg accgtagagc tttcttatgg ttaatgggct
     1501 cattggtcgc ctcagcatgg ttggcgctgt ggatctggga tcgctcgcct tatagccgct
     1561 acctcggtca cgaacagttg ggagagatcg gcgcggcttc gctccttctg cccatcacgc
     1621 tttaccttgt cggttggacg ctaatgacgg tggccatgat gttgccaacc acgctgccgt
     1681 tgctggacat cttccggcgc ctaaccgtgc ggcggcaaga gcgttcacaa cttatggccc
     1741 tggtgattgc gggctatcta ggcgtgtgga tgggattcgg tgtggctgcc cacgtatttg
     1801 attgggggtt gcatcaaatt gttgagcgca tttcgtggct tgaaacgaat gcgtgggtgg
     1861 tcggagcggg cactttgctt ttggccggga gttttcagtt tagccgcttg aaataccgct
     1921 gcctggataa atgtcgcgcg cccttgagct tcgtcatgca atactggcga ggtcgtcatg
     1981 atcggcgaaa cgcgcttttg ctcggcatcc atcatggcat tttttgtgtg ggctgctgct
     2041 gggcgttgat gctcttgatg tttgcggtgg gtgtcgggaa tgtcgcctgg atgttagtgc
     2101 tgggtgcggt tatggccgtg gagaagaata tgccgtgggg acggaaactg agtgccccgt
     2161 tgggtgtggt cttgctggcg tggggcggtt tcattctgct gaatcatttc cgtttgtagc
     2221 aaaataagtc ctgttacggc tactcgtttt tgtacccgcg aagccaatag gaattcgctt
     2281 ttgcaagaca atcaatgccc caccgttatg gtgaattcat ggccaaacca tctcgagatc
     2341 tttttcgggt tactcttttg gggagtgggg cgccgccccc gagaatggat cgcttcggac
     2401 ccagcac
//
LOCUS       DASSWZ010000145         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1009394, whole genome shotgun sequence.
ACCESSION   DASSWZ010000145 DASSWZ010000000
VERSION     DASSWZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009394"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFN58_03555"
     CDS             complement(<1..452)
                     /locus_tag="VFN58_03555"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET8917099.1"
                     /translation="MPSQYVEELNTAERAAREAGRIISALFKGKFDVHEKSKNNPVTT
                     ADLEANRKIREILQGRFPEDGWLSEEDSDDSRRLTSSRVWIIDPIDGTKEFIEGVPQF
                     AVSIGFVVRGRPKVAVIYNPIEDRLYRAAAGQGAELNGERIRVTPRREV"
     gene            complement(599..1189)
                     /gene="pncA"
                     /locus_tag="VFN58_03560"
     CDS             complement(599..1189)
                     /gene="pncA"
                     /locus_tag="VFN58_03560"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015899029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HET8917100.1"
                     /translation="MPSKDALIVVDVQNDFCPGGALAVKDGDQVVAVLNRYIDKFTRG
                     GLPIFATRDWHPAKTSHFNTNGGPWPPHCIQGSQGAQFHPDLKLPPDTVIVSAGMGSD
                     EDGYSGFLGIDDSGTKLIDLLRQRGIERIFVGGLATDYCVKHTVLDGLREKFKVVLLT
                     DSVRGVNLKPGDSERAIAEMLHAGAEIVADLQQVAE"
     gene            1254..1967
                     /locus_tag="VFN58_03565"
     CDS             1254..1967
                     /locus_tag="VFN58_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET8917101.1"
                     /translation="MAIKAIFFDAAGTLIKPARRVGETYAVLAQKYGVEPSAAEITER
                     FRLCFHSAPPLAFSGTPATRIEDLERAWWKELVRRVFEPWDGFQRFDDYFAELFAYFA
                     QPDAWALYPEVAETLPVLERRGLVLSVISNFDSRLIGILQGLGAAHWFEHIFVSSRVG
                     YAKPDRQIFHTALERHSLEAGDALHVGDSEEKDLLGANRAGLKGVLVERNGAGNSNLS
                     PRITSLRSIPSLLDEFDQR"
     gene            2090..3073
                     /locus_tag="VFN58_03570"
     CDS             2090..3073
                     /locus_tag="VFN58_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917102.1"
                     /translation="MAKHRITMGLPYHPRTRALIDREISIDGYELDITHDPSAGERHY
                     RFAQGEWDVGEYSAATFLRTREKGFRYIALPIFFERGPRQRNIYFCEGKLNHPSELKG
                     KKIGCFRYGATAVVWARGFLLDEYSLKTTDMNWYVSGREVYIGSDLPVKVERLDPSPP
                     FGQEKPHLAKLVSAGELHAALVAGDPGYPGLFGGGTLPKAMGEFAGVKPLFDDPEEII
                     RWVRRTGIYPIIHLIALKEEVAERYPDLPDKLLEAFREAKKLSTKHLTAEQIEGHERE
                     KAALGEDPYAYVLGETEKRTMAALARYQIEQGLLKQAPPVESLFVRQAFAQ"
     gene            3250..3933
                     /locus_tag="VFN58_03575"
     CDS             3250..3933
                     /locus_tag="VFN58_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET8917103.1"
                     /translation="MKNKRNNRTGLKNAVVAAALQEERRRLSRELHDRVLQLLTTIQL
                     RSEICLNELIGKPEQLERELKTIAEASHKAATEIRSLLLEKQVIYLAAGTLERRLKDE
                     MEIFRARTGLKLKFECAIDAHNLPYDVEQELYFALREGIINAIRHSRATELNLSLAQN
                     QDACCVDLRDNGVGFDKSSVAAGGGFGLKGMRERIEKVGGHLAIETAPGKGTCISIEV
                     PLRARTNTK"
     gene            complement(3984..4481)
                     /locus_tag="VFN58_03580"
     CDS             complement(3984..4481)
                     /locus_tag="VFN58_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008899475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8917104.1"
                     /translation="MSATDTPGFQIRSATESDIPIILSFIKKLARYERLSHEVVATEE
                     LLRETLFGNRQTAEVAIGYLGTQPVGFVLFFHNYSTFLGKPGLYIEDLFVDEEYRRRG
                     CGRALLLHVARLAKERDCGRLEWAVLDWNQPAIDFYKKLGALPMSEWTVFRITGKRLD
                     ELAGQ"
     gene            complement(4514..>6467)
                     /locus_tag="VFN58_03585"
     CDS             complement(4514..>6467)
                     /locus_tag="VFN58_03585"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET8917105.1"
                     /translation="VRYVGEPVAALAAADMEIAEEAMSLVDVQYEEMAPVIGIDAARA
                     NDAPLIHEDRSDNICSHERVERGNIREGFAQSDLVVEDHFTFPMVYHYAMEPHSVIAH
                     WNDEGVTVWSSAQHPFQVRNDIARLFHLPPSRVRMVISYLGGGYGSKSYTKFEPLVVA
                     LARKASAPVRVCNSVSESMLTVRRHAAQVRVKTGFKQDGAIMAREAEIYLDTGAYDDN
                     GPQVTIRAATRVLGPYRIPNILTNAYSVYTTSGSAGSFRAIGAPQVIFAGESQIDIAA
                     ARLGIDPAELRLKNLLRKDEELRPGLKGIDANLASGLKRLINASQWRRSAKKQRAPVG
                     LACAATNAGATPVSVAMARMQSDGVVNLLAGSTEMGQGVRTVLSQIAAEELTVPLDQI
                     RILGADTKVTPYDSSTGSSRSTTCMGNAVQAAARDLKKQLIKIAADALRVPQRQVKLQ
                     DGALICGEARLSFKETLERRFGKGSGGELIGRGETGPDITDNKLPVFWEVGMGMVELA
                     VDQETGQVRIKRLISVADVGKAIHPEGCIGQEEGAAMMGIGHTFFEEMVYDANGQLLN
                     PNLVDYRLPTFDDVPEEFHTVLVENHDGPGPYGVRGMGEGGLLSVAPSATNAFARATG
                     VRIRDLPLTPERVWRALKQHKAESST"
BASE COUNT         1430 a         1872 c         1722 g         1443 t
ORIGIN      
        1 acttctcgtc ggggcgtcac gcgaattcgt tctccattga gctccgcccc ttgccccgca
       61 gcggctcggt acagtcggtc ctcaatcgga ttataaatca ccgccacttt cggtcggccc
      121 cgcacgacga aaccaatcga caccgcaaac tgcggtaccc cttcaataaa ctctttggtg
      181 ccgtcgatcg gatcgatgat ccaaaccctt gatgacgtca atctgcgaga atcatcgctg
      241 tcctcttcag aaagccagcc gtcttcaggg aatcgtccct gaagaatttc acggatcttc
      301 cggttggctt cgagatcggc cgtcgtcacc gggttgttct tacttttctc gtggacatca
      361 aacttaccct tgaacagcgc gctgatgatc cgccccgctt cgcgagcagc ccgctcggca
      421 gtgtttagtt cttcaacata ttgagaaggc atgacaaccc ttttccttgc gactcgatca
      481 atgggatcat taaaaatgcc agatcggcta tttcgacaat tgctttaccg aaggctaaac
      541 agggaatgaa acggattgag ataaaagccg gccgctgtgc ccataagtgt acgcagtttt
      601 actcggcaac ttgctgcagg tccgctacaa tctctgcgcc ggcatgaagc atttcggcga
      661 tggcgcgttc cgaatctcca ggcttcagat tcacgccgcg aacggagtcg gtaagcaata
      721 caaccttgaa tttctctctg agaccgtcga gcacggtatg cttaacgcaa tagtccgttg
      781 ccaatccgcc gacaaaaatg cgttcgatac ctcgctgtcg taaaaggtca attaattttg
      841 ttccgctgtc gtcaattccg agaaagcctg aatagccgtc ttcatccgaa cccattcctg
      901 ccgatacgat caccgtgtcc ggcggtagct tcaggtccgg atgaaattgg gctccctggc
      961 tcccttgaat acagtggggc ggccaggggc caccattggt attgaaatga ctcgtctttg
     1021 ccggatgcca atcgcgagtg gcaaagatcg gcaagccgcc tctggtgaat ttatcaatgt
     1081 agcggttaag cacagcgaca acctggtcgc cgtccttcac ggccagagca ccccccggac
     1141 agaaatcgtt ttggacatcg accacaatta acgcgtcttt acttggcatc gatttccatt
     1201 atagagaatg atagcttggt tgcaattggc tagtttaaca cttagcgcag aacatggcca
     1261 tcaaggcaat cttttttgac gccgcgggga cacttatcaa acccgcccgc cgggtcggcg
     1321 aaacctacgc cgtcttggcg cagaaatacg gcgtcgaacc ttcagcagcg gagatcacag
     1381 agagatttcg actctgcttt cattctgcac ctcccctggc cttttccggt acgccggcaa
     1441 cgcgtatcga agacctggaa cgcgcctggt ggaaggagct ggtgcggcgc gtctttgaac
     1501 cttgggatgg gtttcagcga tttgacgact actttgccga gcttttcgct tacttcgctc
     1561 aacccgatgc ctgggccctc tacccggagg tagccgaaac tttgccggtc ttggaaaggc
     1621 gcggcctggt cctcagtgtg atctcaaact ttgactcgcg tctgattggc atcctccaag
     1681 gccttggcgc agctcattgg ttcgagcata tttttgtctc aagccgcgtt ggttacgcga
     1741 aacccgatcg acagattttt cacacagctc ttgaacgcca cagtcttgaa gccggtgacg
     1801 ctctccacgt cggcgatagt gaggagaagg atcttctcgg cgccaacagg gccgggctga
     1861 agggggtctt agtcgaacgt aacggcgcag gcaattcgaa tctttcgcca cgaatcacga
     1921 gcttgagaag cattccttcc cttttggacg aattcgatca acgctaaaag taacttgaca
     1981 ggctcagacg gctgcgatac acggctgcta gctcccggag atttaatgtc agaatccagc
     2041 tgggaacggg tcgacacgga taacgatccg cagtaaaaag aggacaccga tggccaaaca
     2101 tcgcatcaca atgggtcttc cgtatcatcc acgcacacga gcgctgattg acagggagat
     2161 tagcatcgac ggctacgagc ttgatattac gcatgaccca agcgccggcg aacgccacta
     2221 tcgcttcgcc caaggggagt gggacgtggg agaatattcg gcagccacct ttcttcggac
     2281 gcgcgagaaa ggttttcgct atatcgcatt gccgatcttt ttcgagcgcg ggccgcgcca
     2341 gcgcaacatc tacttctgcg aaggcaaact caatcacccc agcgagctca aaggaaagaa
     2401 aatcggctgc tttcgctacg gcgccacggc ggtggtttgg gcccgtggtt ttctgctcga
     2461 cgaatatagc ctcaaaacca ccgacatgaa ttggtacgtg tcgggtcggg aagtttacat
     2521 cggcagcgat cttcccgtta aagtagaacg cctcgatccg tctccgcctt tcggacaaga
     2581 aaaaccgcac ctagcgaagt tggtcagcgc gggtgagcta cacgccgctc tggtcgccgg
     2641 cgatccgggc tatccggggc tttttggcgg gggaactttg cccaaagcca tgggtgagtt
     2701 tgccggcgtg aagccccttt ttgatgaccc tgaggaaatc atccgctggg ttcgacgaac
     2761 ggggatttac ccgatcattc atcttatcgc tttgaaagaa gaagtcgcag agaggtatcc
     2821 ggatctaccc gacaaactgc tcgaagcctt tcgcgaagcg aagaaacttt caacaaagca
     2881 cctgacagct gagcaaatcg aggggcatga acgagagaaa gcggcgctcg gcgaagatcc
     2941 ctacgcctat gtccttggag aaactgagaa acgaacgatg gcggctttgg ctcgttatca
     3001 gatcgagcaa gggcttttga aacaggcacc gccggtggaa agcttgtttg tccggcaggc
     3061 attcgctcag tgaaatgctc ttcggttgaa acgtacgacc tgtgctacgt gcccgaaaca
     3121 ttcttagaag ttggcgtagc ctcacgcttc tctgcccgtg cccttcgagg ttcattccac
     3181 gggcgacact ctctcgtgat ttttcttctg agcgagaata tgccgggtta gccgcgtcat
     3241 tggataaact tgaagaacaa acgcaataac cggaccggcc tcaagaacgc agtcgtcgcc
     3301 gcggctcttc aggaagagcg gcggcgcctc tcccgggagt tgcatgaccg tgtgctccag
     3361 ctgctgacca cgattcagtt gcgttccgag atttgcctta acgaactgat cggcaaaccc
     3421 gagcagcttg agcgggaact taaaacgatc gcagaggctt cccacaaagc cgcgacggag
     3481 attcggagtc ttttgctgga gaagcaggtc atctatttgg ccgccggcac tctggaacga
     3541 agactcaagg atgaaatgga gatcttccgc gcgcggacag gattgaaact caagttcgaa
     3601 tgcgcaattg acgcgcacaa tcttccctat gacgtcgagc aagagttata ttttgcgtta
     3661 cgagagggaa ttatcaatgc catccgccac tctcgcgcaa ccgaattgaa tttgtccctg
     3721 gctcagaacc aagacgcttg ctgcgtcgac ctgagggaca atggagttgg ctttgacaag
     3781 agttcggtgg ccgcaggcgg cggttttggc cttaagggaa tgagagaaag aatcgagaag
     3841 gtcggcggtc atctcgccat tgaaacggct ccaggcaaag ggacttgtat ctctattgaa
     3901 gtgcctctcc gagcccggac aaacacaaag taattcagga agacgtccca agggcgtggc
     3961 cggaagctaa acgccgtctc cggttattga cccgccagtt catctaggcg cttgccggtg
     4021 attcgaaaca ccgtccactc gctcatcggc aaagctccta gtttcttgta aaagtcgatc
     4081 gccggctgat tccagtcgag caccgcccat tcgagccgac cgcaatcacg ttccttcgcc
     4141 agtcgcgcga cgtgcagcaa taacgcccga ccgcacccgc gcctccggta ttcttcatcc
     4201 acgaagagat cctcgatgta gaggcccggt ttgcctagga atgtggaata gttgtgaaag
     4261 aatagaacaa acccgacggg ctgggttccc aggtatccaa tggcaacttc cgccgtttgc
     4321 cggttaccga acagagtctc ccggagtagc tcttcggtgg cgacgacttc atgcgacagc
     4381 cgctcgtagc gcgcaagctt cttgataaaa gaaagaataa tcggaatatc agattcggtg
     4441 gctgatctga tttggaatcc tggagtgtcc gtcgcgctca cggttacctc ttgttttagt
     4501 cgaagtgtgt cgatcaggtt gatgattccg ctttgtgttg cttcaacgcc cgccacaccc
     4561 gctccggagt cagggggagg tcccttatac ggacaccggt ggcccgcgca aaggcgttcg
     4621 tcgccgacgg cgccactgac agaagccctc cctcacccat ccctcggaca ccgtagggac
     4681 ccggtccgtc gtggttttcc acgagcaccg tatgaaattc ctcgggcaca tcgtcgaagg
     4741 tcggcaatcg atagtcgaca agatttggat tgagcaactg gccattggcg tcatacacca
     4801 tctcttcaaa gaacgtgtga ccgatcccca tcatcgcggc tccctcttcc tggccgatgc
     4861 atccttcggg atgaatcgcc tttccgacat cggccaccga gatcagccgt ttgatccgca
     4921 cctggccggt ctcttggtca acagccaact ccaccatccc cattcccact tcccaaaaaa
     4981 ccggcaactt gttgtccgta atatcgggtc ctgtctcgcc gcgaccgatg agctcgccgc
     5041 ccgagccttt gccaaaccgc cgctccaaag tctctttgaa acttagtcgc gcttccccgc
     5101 aaatcaacgc gccatcctgt agcttgactt gcctctgggg cacgcgcaat gcgtcggcgg
     5161 cgattttaat gagctgcttt ttgagatcac gcgccgcggc ctgtaccgcg ttgcccatgc
     5221 aagtcgtcga ccggctggaa ccggtggagc tatcgtaagg ggtgactttt gtatccgcgc
     5281 ccagaatgcg aatctgatcc aacggcaccg taagctcttc ggccgcgatc tgcgaaagaa
     5341 ccgttcgtac accctgaccc atctccgtgc tcccggccaa gagattcacg acgccgtctg
     5401 attgcatgcg agccatcgcg accgatacgg gggttgcgcc cgcattggtc gcggcgcagg
     5461 cgaggcccac gggcgcgcgt tgtttcttag ccgaccgtct ccattggctg gcgttgataa
     5521 gccttttcaa tcctgaagcc aaatttgcgt cgatgccttt caatccggga cgaagctctt
     5581 catcttttct cagtaaattc ttaagccgga gttccgccgg gtcgattcct agccgggccg
     5641 ccgcgatgtc gatctgtgat tctccagcga agatcacctg cggcgcgccg atggcgcgaa
     5701 aagaacctgc ggagccggag gtcgtataga cggaataggc gttggtcaag atattgggaa
     5761 tccggtaagg gcccaagacc cgcgtcgccg cccggatcgt cacctgcggc ccattgtcgt
     5821 cgtaggcgcc cgtatccaga taaatttcgg cctcccgggc catgatagcg ccgtcttgct
     5881 tgaaaccggt tttaaccctc acctgggctg cgtgccgccg caccgtcagc atcgattcgg
     5941 agactgaatt gcacacgcgt acaggcgcgg acgccttacg cgccagtgca accaccagcg
     6001 gttcaaactt ggtgtacgac ttgctgccgt agccaccacc taagtaggaa atcaccatcc
     6061 gaactcgaga tggcgggaga tgaaatagtc tcgcaatgtc gttacgcacc tggaacggat
     6121 gttgagccga agaccagacc gtcacaccct catcgttcca atgggcgatc acagagtgag
     6181 gctccatggc gtagtggtaa accatcggga atgtaaaatg atcttcaacg accagatcgg
     6241 attgagcaaa gccttcccgt atgttgccgc gttcgactct ttcgtggctg cagatgttgt
     6301 cgctgcgatc ctcatgtatc agcggcgcgt cgttggcgcg agccgcatcg atcccgatca
     6361 ccggggccat ttcttcgtat tgcacgtcga cgagcgacat cgcctcctca gctatctcca
     6421 tatcggctgc cgccagcgcc gccaccggct cgccgacgta acgaact
//
LOCUS       DASSWZ010000146         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_358360, whole genome shotgun sequence.
ACCESSION   DASSWZ010000146 DASSWZ010000000
VERSION     DASSWZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358360"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..452)
                     /locus_tag="VFN58_03590"
     CDS             complement(15..452)
                     /locus_tag="VFN58_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917106.1"
                     /translation="MKIRNLILSAALLTWASTASAQTMTEITGRQWMASDKDVREIFV
                     LGYVNGLEQLLSHYNFQIRSGIGKYFDAHVLDEALYKKLSNEPELRSGPVRDTLMSVL
                     NDYAVLTDRVGNNLPSWQKLMSTSECAEFVKALYENQKSRMGF"
     gene            775..1440
                     /locus_tag="VFN58_03595"
     CDS             775..1440
                     /locus_tag="VFN58_03595"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HET8917107.1"
                     /translation="MASSKSLPRDFTRRLFLIVFLIHACSDPTAEIRFYAAGKTVRSV
                     YVVHDAWHSAVVIKKADIPATVLPEIRDFPAAEYLEFSWGDRDYFPAPDGGLGLALKA
                     AFWSSGSILHVVGFRDAVANTFPGAEIIEIALSEEAFQGLIKFISDTFSRPHPPAPAE
                     PRPGLFPNGRFYSAEGKFSVLRTCNTWVAEALSAAGLPIRPGYVITAANLGNQVRPFA
                     IRK"
     gene            1599..2141
                     /locus_tag="VFN58_03600"
     CDS             1599..2141
                     /locus_tag="VFN58_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET8917108.1"
                     /translation="MGRLPDIDETKLSSEQRRIYDQIMAVRGHVRGPFGVWLRNAELC
                     ENTLKLQEMFASRVKLERRLVQLMILVAARLATAHYAWFIHEPHALNLGISPEIIEAI
                     RERRTPEFTREDERLVYDITMELNTTRSLSEANYQRGMAILGEQSMVELVSAIGFYVM
                     VAMTLNAFAVPIPGGKERLA"
     gene            2233..2670
                     /gene="mscL"
                     /locus_tag="VFN58_03605"
     CDS             2233..2670
                     /gene="mscL"
                     /locus_tag="VFN58_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET8917109.1"
                     /translation="MLKEFKEFAMRGSVLDMAVGIVIGAAFGKIIASLVDDIIMPPIG
                     LALGNVDFANLFLNISGKEYSSLAAAKAAGAATINYGVFFNTILSFLIIAFVIFLLIR
                     QVNAMQRPKGAPAPATKDCPHCLSSIPIKATRCPHCTSELKAA"
     gene            complement(2702..3910)
                     /locus_tag="VFN58_03610"
     CDS             complement(2702..3910)
                     /locus_tag="VFN58_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8917110.1"
                     /translation="MADETQGKLDLSAICHVGPGTAGGEMFRRYWLAISRTEDLKDIP
                     QSVKVLGEELVLFRDGSGRLGLMGLHCSHRGTSLEYGDLEERGIRCPYHGWLYDTEGN
                     CLEQPLEPKGSTFCQKVKHPAYRVKELGGLIFAYLGPNQSAPPVLPRYSALVRDDGVR
                     LVLPPRHWDYNWFNFFENTIDSLHAFFLHKPSRADRSWENAFWDYPGDHHIEAMRTDY
                     GLKTIVHWPGPTPDTLYVRLTTLALPTVFSLGGRGVEEEGFERLLFVTPADDDNFMVY
                     SSDFVPRSKEDVIKKRDQSRYAPPAAEPVKEYDKRKHVPYRGQVWKEDYVCQSTQGKI
                     GYRHEQLGTSDRGVILLRKLLMEAIETVQKGGEPQGIIPKEKEDQLITLDAFRRILPK
                     PAVAELLRPN"
     gene            complement(3932..>4553)
                     /locus_tag="VFN58_03615"
     CDS             complement(3932..>4553)
                     /locus_tag="VFN58_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917111.1"
                     /translation="GIRSIADIKGKKIAVTGLGSTTDYAARAILKHHHFDADKEVSIV
                     AVGSESVFPALQRGAVDAAVLWAPGFAVAEKLGMVRIQSLADILELPGSGVVVSDQLI
                     KENPLLIKKFLRGTLKGFQYVHDPKNRDEIVNAIMKDFNLEPDVAETNYRFILSILSA
                     DGTISRRSMENGIELTRQRVKTSEPTGELVKKMYDFALLEEVQRGK"
BASE COUNT         1004 a         1227 c         1195 g         1127 t
ORIGIN      
        1 tcgcagaatt cggcttagaa tcccatcctg gatttctggt tttcgtacaa ggctttcacg
       61 aactcggcgc attcggatgt cgacatgagc ttctgccagg aggggagatt gttgcccact
      121 ctgtcggtga gaaccgcata atcgttaagg acactcatga gggtgtctct gaccgggccg
      181 cttctgagtt ccggttcatt gcttagtttt ttatacaacg cctcatcaag aacgtgagcg
      241 tcgaaatatt taccgatgcc gctgcggatc tgaaaattat agtgcgagag taactgctcc
      301 aatccgttta cgtatcccaa gacaaagatt tctcgaacgt ccttgtctga tgccatccat
      361 tgtcgcccgg tgatttctgt catcgtctgg gccgaagcgg tgcttgccca cgttaaaagc
      421 gcggcagata ggatcaagtt tctaattttc attcggtcct ccgtccggcg aaacgataga
      481 gcacccgcta tgccatgagg gagaaccggc aattatggag cttggaggat ggttcgagaa
      541 ctagaggtaa attccgctgt gacggcctgg aggtagaaat gccttctgta agccatcggg
      601 tgcgttttag cccgacgggt cggtcaaata cgcatttgcc gaactaggag atagcttaag
      661 actcacacag gttgcacgcg gaaaacgctc aaacagtaag aagagactcc gatccttttt
      721 cttccctcga agcttgtatt gattttccga cgggcaacgc gatacttgac ttgcgtggcc
      781 tcctcaaagt cactcccacg ggacttcact cgccggctct ttcttatcgt cttcttgatc
      841 catgcctgtt cagacccaac ggcagaaatc cgcttttacg cagcgggaaa gactgtcagg
      901 agtgtttatg tcgttcatga cgcttggcat tccgccgtgg tgataaaaaa ggctgatatc
      961 cccgctactg ttttgcccga aattagggat tttccagctg ccgagtattt ggaattcagc
     1021 tggggagaca gggattactt cccggctccg gacggagggc ttggcttggc gctcaaggcg
     1081 gcgttctggt caagcggcag cattcttcac gttgttggtt tcagagacgc tgtggcaaat
     1141 acttttcccg gcgccgagat tattgaaatc gctttatcgg aggaggcttt ccaaggtttg
     1201 atcaaattca tatcagatac gttttcaaga ccgcatccgc cggcgccggc tgagccgcga
     1261 ccaggccttt ttccaaacgg caggttctat tctgcagaag gcaaattcag cgtcttgagg
     1321 acatgcaaca cctgggtcgc ggaggcatta agcgccgccg gactaccgat caggccgggc
     1381 tacgtaatca ctgcggcaaa cctcggtaac caagtgcgac ccttcgcaat ccgcaaatag
     1441 cgcccgtcac agacgcggct ctattactaa ttgtgcgggc cagcccgatc atgttttgga
     1501 ggcagcttga attatgtaag cttccagagt agtcaattgg cttgatctga tacagttccc
     1561 cgaggggttg ttcggccatc agcagaaagg acgaggccat gggacgcttg cccgatatcg
     1621 acgaaaccaa actctcttcc gagcagcgac gaatctacga tcaaataatg gcggtgcgcg
     1681 gccacgtgcg cggccccttc ggggtttggc tgcgcaatgc tgagctctgc gaaaacacgc
     1741 tcaagttgca ggaaatgttt gcttcacgag tgaagctcga acgacggttg gttcagttga
     1801 tgatcttggt tgcggcgcgt ttggctaccg cgcattatgc atggtttata cacgagcccc
     1861 acgcgctcaa cctcggcatc tcgcctgaga tcatcgaggc catccgcgag cggcgcacac
     1921 cggagtttac ccgcgaggat gagcgcctcg tttatgacat cactatggaa ctcaacacca
     1981 cccggtcgct atctgaggca aactaccaac ggggcatggc gattttggga gagcagtcaa
     2041 tggtcgagct ggtcagcgcg attggtttct acgtgatggt ggccatgacg ctcaacgctt
     2101 ttgcggttcc gattcccgga gggaaggagc gattggcgta gatcactaag tgtgaagcat
     2161 tttcaagaag gcggaaagtt tttctccagt catcgtcaga agaaagatta attcaacata
     2221 gggggtgtga caatgctaaa agagtttaaa gagtttgcta tgcgcggaag cgtactcgac
     2281 atggccgtcg gcatcgttat tggagcggct ttcggcaaga ttatcgcttc actggtggac
     2341 gacatcatca tgccgccgat tggtttggcg ctcggaaatg tcgattttgc caacctgttt
     2401 ctcaacattt cgggcaagga atattccagt ctcgctgcgg cgaaggcagc tggtgcggcg
     2461 accatcaact acggggtatt tttcaatacc atccttagct tcctgatcat cgctttcgtc
     2521 atcttcctgt tgattcggca ggtcaacgcc atgcagagac caaagggagc gccggcgccg
     2581 gcaacgaagg attgccccca ttgtttatcc agcattccca taaaggccac tcgctgcccg
     2641 cattgcacct ctgaactcaa ggcggcgtag aaaacgaaat gctggtgttg ccgcgcccgc
     2701 gctaattagg gcgcagcaat tcagccaccg cgggcttcgg caggatacgg cgaaaggcgt
     2761 caagggtaat caattgatct tccttttctt ttggaatgat tccctggggt tcgccacctt
     2821 tttgcaccgt ttcaatcgct tccatcagga gttttcgcag tagtataacg ccccggtccg
     2881 acgttcccaa ttgctcgtgt ctatagccga ttttcccttg cgtcgattga cagacatagt
     2941 cttccttcca tacttgccct ctgtaaggaa cgtgcttgcg cttgtcgtat tcttttaccg
     3001 gttctgcggc cggcggcgcg taacgagatt gatccctctt cttaatcacg tcctccttag
     3061 atcggggaac aaaatcgctg ctgtaaacca tgaagttgtc gtcatcggcc ggcgttacga
     3121 acagcaagcg ctcaaagccc tcttcttcta ctccccggcc gcccagcgag aaaaccgtcg
     3181 gcagcgcaag ggtcgtcagc ctcacataca gggtatccgg cgttgggcct ggccagtgga
     3241 cgattgtttt caggccatag tcggtccgca tcgcctcgat gtggtggtcg ccagggtaat
     3301 cccagaatgc gttttcccaa gaacggtcgg ctcggctcgg cttgtgtaag aagaacgcgt
     3361 gtagagaatc aatcgtattc tcaaaaaaat tgaaccagtt gtaatcccaa tgccgcggcg
     3421 gtaacaccaa tcgaacgcca tcgtctctga cgagcgcgga ataacgcggt aacacgggtg
     3481 gggcgctctg gttcgggccc agatacgcga agattaggcc gcccaactct tttactcgat
     3541 aagccggatg tttgactttt tgacagaagg tgctcccctt cggctccagc ggctgctcta
     3601 aacaatttcc ttcggtatca taaagccagc cgtgatacgg gcaccgtatg ccgcgttctt
     3661 cgagatcacc gtattccaac gatgttccgc gatgggaaca gtgcaatccc atgagtccaa
     3721 ggcgtccgct gccatcgcgg aaaagtacca gttcctcacc cagaactttg accgactgag
     3781 gaatatcctt caaatcctcg gtgcgcgaaa ttgccagcca gtagcgccga aacatttcgc
     3841 caccggcggt gccgggccca acatgacaaa ttgccgaaag atcgagcttg ccttgggttt
     3901 cgtccgccat gatccatctc ctgatgtcta tttatttgcc tctctgaact tcttccagca
     3961 aagcaaaatc gtacatcttt ttcacgagct caccggtcgg ctccgaggtc ttgacgcgtt
     4021 ggcgcgtaag ttcgattccg ttttccatcg agcggcgcga gatcgtgcca tccgcggaca
     4081 ggatactcag aataaacctg tagttggttt ccgccacgtc cggttctaaa ttgaaatctt
     4141 tcatgatggc gttgacgatt tcgtccctgt tcttgggatc atgcacgtac tggaagccct
     4201 ttagagttcc gcgcaggaat ttttttatca gtagagggtt ctctttgatt agctgatcgg
     4261 ataccacgac acccgagccg ggcaattcta ggatgtcggc gagagactgg atcctcacca
     4321 tcccgagctt ttcggcgacg gcgaaaccgg gagcccacaa caccgccgca tcaacggctc
     4381 ctctctggag cgccggaaaa acactctcgc tacccacggc tacgatcgag acttctttat
     4441 cggcgtcaaa atgatggtgc ttcagaatag ctctcgcggc atagtccgtc gtgcttccga
     4501 ggcccgtgac ggctatcttt ttccccttta tgtcggcaat cgaacgaatt ccg
//
LOCUS       DASSWZ010000147         5715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_783135, whole genome shotgun sequence.
ACCESSION   DASSWZ010000147 DASSWZ010000000
VERSION     DASSWZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783135"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /gene="glyA"
                     /locus_tag="VFN58_03620"
     CDS             complement(<1..137)
                     /gene="glyA"
                     /locus_tag="VFN58_03620"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942252.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET8917112.1"
                     /translation="MSFLEQIDPEVFSAIQKETDRLEQNLELIASENVVSEAVLEAQG
                     CV"
     gene            complement(159..632)
                     /gene="rpiB"
                     /locus_tag="VFN58_03625"
     CDS             complement(159..632)
                     /gene="rpiB"
                     /locus_tag="VFN58_03625"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET8917113.1"
                     /translation="MIAIGSDHGGVELKDYLVGFLRCRGADVRDFGTQGRESVDYPDF
                     GREVSLRVARGEAEWGILICTSGIGMSIAANKFPGVRAALVVDLDGARTSREHNDANI
                     LVLSGAKTDKPVAQEIVEMWLETPFAGGRHQRRVEKITQIEQDLGIRIDNDQAKK"
     gene            complement(801..1037)
                     /gene="acpP"
                     /locus_tag="VFN58_03630"
     CDS             complement(801..1037)
                     /gene="acpP"
                     /locus_tag="VFN58_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET8917114.1"
                     /translation="MAASVQTRVKEIVCEQLGVSEEEVTPEASFIEDLGADSLDIVEL
                     VMALEEEYEMEISDEDAEKIKTVQDVITYIESHR"
     gene            complement(1067..1810)
                     /gene="fabG"
                     /locus_tag="VFN58_03635"
     CDS             complement(1067..1810)
                     /gene="fabG"
                     /locus_tag="VFN58_03635"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET8917115.1"
                     /translation="MSLEGQIGLVTGGSRGIGRAVVLALGRLGAHVIINYRSNQTAAT
                     ETLHELVKRGGHGEVDQFDVSNENQIEEAIKKIVDHHKKIDILVNNAGVTSDNLLIRM
                     RSEDWDQVIGTNLKGTVFCTKSASRVMIRQRYGRIINLSSVVGQMGNAGQSLYAATKA
                     GIIGFTKAMAREVASRGVTVNAVAPGFIETEMTAQLSAKMQEAFLHSIPLGRFGTCDE
                     VAELVAFLAGPGAGYITGQVLSVNGGLYM"
     gene            complement(1807..2754)
                     /gene="fabD"
                     /locus_tag="VFN58_03640"
     CDS             complement(1807..2754)
                     /gene="fabD"
                     /locus_tag="VFN58_03640"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET8917116.1"
                     /translation="MKQSGKVAFVFPGQGSQYVGMGKDLYAQFAVAGRVFGEADEALG
                     FPLSQLCFAGPESDLKLTENTQPAILTVSVAALRVLESETSLKADFVAGHSLGEYTSL
                     VCAGALGFQDAVRVVRERGRLMQQAVPAGAGAMAVILGLGMEAVQSLCEAVSNGEVVA
                     PANYNGGGQIVIAGSKAAVTRAMSIAKEYGAKRVLDLPVSAPFHCQLMRPAAEGLGKV
                     LDDVTVYPFSLGVITNVEADVNLDAGRVKSLLIEQAVQPVRWEESVRKLESLGCVRVW
                     EIGPGKVLKGLINRINRALQVDNFEAPQDLGKLLPEELA"
     gene            complement(2879..3862)
                     /gene="plsX"
                     /locus_tag="VFN58_03645"
                     /pseudo
     CDS             complement(2879..3862)
                     /gene="plsX"
                     /locus_tag="VFN58_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
     gene            complement(3881..4063)
                     /gene="rpmF"
                     /locus_tag="VFN58_03650"
     CDS             complement(3881..4063)
                     /gene="rpmF"
                     /locus_tag="VFN58_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET8917117.1"
                     /translation="MPVPKRRTSRRVKNQRRSHDALTAPQWSTCPKCGETVLPHRACS
                     GCGFYRGRIVIKTDES"
     gene            complement(4128..4664)
                     /locus_tag="VFN58_03655"
     CDS             complement(4128..4664)
                     /locus_tag="VFN58_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET8917118.1"
                     /translation="MKIPVDEITESAKEISFAERIDGLNDLYKNGQFRDFGFPPFLEV
                     DLVYYRSGQEIFFKGSFGGKLEGSCSRCLRSYSFPLNKAFDFVLSPDPYKPGRKIEEL
                     KREDLGLSYYSTEEINLAPLIKEQVMLALPTRPLCHENCRGLCGSCGVDLNKETCDCI
                     GPTADPRMAIFRTLKVGR"
     gene            4909..>5715
                     /locus_tag="VFN58_03660"
     CDS             4909..>5715
                     /locus_tag="VFN58_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HET8917119.1"
                     /translation="MSFPVTLRKQWHGRRYNSYNRLLKDTFDARVYKIGLRLDFTCPN
                     RDGTVAVGGCIYCNNSSHTPQNYEPRTSVTEQLERGATAIQRRHRAEKFIAYFQSYTN
                     TYGSVSKLEKLYREALDFRGVVGLSIATRPDCLPEDTLDLLTDLSHHTYLWLEMGLES
                     MQDRTLRWVNRGHGLNEFVETAQRCKKRGLRLCTHLILGFPGESRAEILATTALLNRL
                     QIDGLKLHNLHIIKNTVLEKLYATGGFTLFSQNEYVTLVVDFLERLAPEMV"
BASE COUNT         1325 a         1673 c         1332 g         1385 t
ORIGIN      
        1 acgcaacctt gagcttccaa cactgcttca ctgacaacgt tttccgaagc aatgagctcg
       61 aggttttgct caagcctatc ggtttccttt tgaatcgccg aaaaaacttc gggatcgatt
      121 tgttccagaa aggacatggt cattcttcct tcgcgtagtt atttcttggc ctgatcgttg
      181 tcgatgcgaa taccgagatc ctgttcaatc tgggtaatct tttcgacacg ccgctggtgc
      241 cttccgccgg cgaaaggggt ttcgagccac atttctacta tctcttgtgc gactggttta
      301 tccgtcttcg ccccgcttag aaccaggata ttggcatcat tgtgttcccg gctggttctc
      361 gcgccatcga gatctaccac tagtgcagca cgaaccccgg gaaacttatt ggcggcaatg
      421 gacatgccga tgccgctggt gcagatcagg attccccact cggcctcgcc tcgggcaaca
      481 cgcagagaga cctcgcggcc aaaatctggg tagtcgacgg attcgcgccc ttgggttccg
      541 aagtccctta cgtccgcgcc gcggcaacgg aggaagccga ccaagtagtc cttgagttct
      601 actccaccat ggtcactgcc aatcgcgatc ataactcggg cgcgctctcg attagagttc
      661 ccttggtctt gctagacttg caaagaaaaa aacgcaggac gaggattagc tcaaccctct
      721 gcgatctcca gctcgatttg cggtgactac tttgggccag caccgcacca ttttagttct
      781 tcactagcaa cttggagaaa ttagcggtgg ctctcgatgt aggtgattac gtcctggacg
      841 gtttttattt tttccgcgtc ctcatcggat atctccatct cgtactcctc ttccaacgcc
      901 atcaccaact cgacgatatc cagagaatcc gctcccagat cttctataaa ggacgcttca
      961 ggagtaactt cctcctcact caccccaagt tgttcacaga cgatttcctt caccctggtc
     1021 tgaacagatg ctgccatctt tatctcacct cctgcctacg ttttcctcac atgtaaaggc
     1081 cgccattaac actaagcact tgtccggtaa tatatccagc cccaggcccg gcaagaaaag
     1141 ccacgagctc cgcgacttcg tcgcaggtgc caaaacggcc caggggtata gagtgtagaa
     1201 aagcctcttg catcttggcg gataattgcg cagtcatttc ggtttcaatg aaacccgggg
     1261 caaccgcgtt gacggtaacg cccctcgagg cgacttccct ggccatggct ttggtgaaac
     1321 caataatgcc tgctttggtg gcggcataca gtgattggcc ggcattgccc atctgcccta
     1381 ccaccgacga cagattaatg atcctaccat accgctgacg gatcatgact cgggacgcag
     1441 acttggtgca aaaaactgtg cccttgaggt tggttccgat aacctggtcc caatcctctg
     1501 acctcatccg aatcaagaga ttgtcagagg ttacaccggc attgttgacc agaatatcaa
     1561 tttttttgtg atggtcaacg attttcttaa tggcctcttc tatttgattt tcgtttgaaa
     1621 catcaaactg atccacttcg ccatgccccc cccgtttcac cagttcgtgt aaggtctcag
     1681 tggccgccgt ctgattgctt cgatagttga tgatcacgtg ggccccgagg cgccctagcg
     1741 ctagaacaac cgccctgccg atgccccggc ttccacccgt gactagaccg atttgacctt
     1801 ctagactcat gcgagctctt ctggaaggag tttgccaaga tcttgcggag cctcaaaatt
     1861 atcgacttgc agcgcccgat tgatgcggtt gatcaaaccc ttgagcacct tccccggtcc
     1921 gatctcccat acgcgaacgc aaccgagact ctcaagcttg cggaccgatt cctcccaccg
     1981 tacgggctgc acagcctgtt ctatcaacag agacttcaca cggccggcgt ctaggttcac
     2041 atccgcctcg acgttcgtta tcacaccgag cgaaaaagga taaacggtga catcgtctaa
     2101 aacctttcct aatccttcgg cagccggacg catgagctgg caatgaaagg gagcactgac
     2161 gggtagatcc aaaaccctct ttgcaccata ctccttggca attgacatag cgcgggtaac
     2221 cgcagctttg gagccggcaa tgacaatttg tccgcccccg ttgtaattgg ccggtgccac
     2281 aacctcgcca ttgctgaccg cttcgcaaag ggattgaaca gcttccatcc ccaatcccaa
     2341 aatcaccgcc atagcgcccg caccggcagg caccgcttgc tgcatgagtc ttccccgttc
     2401 acgcacaacc ctgaccgcgt cctggaagcc aagcgcgcca gcacaaacca acgaagtgta
     2461 ttctccgaga ctgtgaccag ccacaaagtc cgcctttaag gaggtttctg attccaaaac
     2521 acgcagagcc gcaactgaaa ctgtcaaaat agccggttgg gtattctcag tgagcttgag
     2581 atcggactcg ggcccagcaa aacaaagttg acttagggga aatcccagtg cctcatccgc
     2641 ttccccaaac acccgccccg cgaccgcaaa ttgcgcgtaa aggtccttcc ccattcccac
     2701 atattgagag ccctgtccag gaaaaacgaa agcaacttta ccgctttgct tcaccttgcc
     2761 gttcctcggt ttcgctctcg ctgctcggtt tatcccctag ggaatcgatc ttcgatttga
     2821 ttcgctgcca aattttgctc ggcaacccat gccttttctc gccgccgctg ccacccattt
     2881 ccccgagcac ttggctgata tgcccgttga tatcctgttg aacttcttcc ttggccgcgc
     2941 ggatagcatt cttaatcgcc cgcggactcg accctccatg agcgacaatc ccgaccccgt
     3001 caaggccgag caagggagcg cccccatact cagcatagtc gagacgccga taggcttctc
     3061 ggagcgaact gcgactcatg aaatatccca gtcgactgga aagattcttc tgaaaagctt
     3121 ccttcaacac gtctcccgcg aaactcgcaa caccttccat agtcttgagg gcgatattgc
     3181 cggtgaatcc atcgcagacg atcacatcga ctttgccatt gaaaacatcc cgtccttcta
     3241 catagccaac gtagttcaat gaggtcgctg caagctcttg gctggccgcc ctggtgagat
     3301 cgtttccctt tccttcttcc tcgccattgc tcaagatccc gacgcgcgga ctggcaatcc
     3361 cctggacttt ctcagcgtag atcgcgccca taagcgcaaa ctgaaccaga tgatgcggtt
     3421 tgcagtctac gttggcgccg gcgtcgataa tgaccgttcc cttcgacaga cccggcacga
     3481 cgatcagaat cgccggccga gctacctgcg gcaacctgcc catgacaaac atccctgtcg
     3541 ccatcatggc accggaattc cccgcgctca ccacagcctg aacttctccg cgtttcatca
     3601 tctcaaaggc gatcctcatg gacgaatcct tttttttgag cgcagagctg ggagattcat
     3661 tcatagcgat catttgagaa gcatggacca cttgaactga agacgatggg gaaggatgac
     3721 gcactagttc gtgttctacg acatcctttt gacccaccaa ggccagatcc acgccgaatt
     3781 ctcgggcagc ccataacgct ccctctacca ctaccccagg agcatggtcc cctcccatgg
     3841 cgtcgacggc gatttttagc atacaaaaaa ggaaggcagg ttaactttcg tcggtcttta
     3901 tcacgattcg accgcggtag aaaccgcagc cggagcaggc gcggtgcggc agaacggttt
     3961 ccccgcactt gggacaagtg ctccactgtg gagcggtcag cgcatcgtgc gatcgccttt
     4021 ggttttttac tcttcttgaa gttcttctct tcggtactgg cattttctta gtcctatctt
     4081 tccctgtcgc tcggcgacag acacaatgca gcaaatgtcg acgcgaacta acgaccgact
     4141 ttgagggttc gaaaaatcgc catccgcgga tcagccgtgg ggccgataca gtcgcaagtc
     4201 tccttgttga ggtcgacgcc gcaacttccg caaagtccgc ggcagttttc gtgacagagc
     4261 ggccgggttg ggagagccaa catgacctgt tcttttatta gaggcgcgag gttaatttcc
     4321 tctgtggaat aataactcaa ccccaaatcc tcccttttca gctcttcaat tttgcgccct
     4381 ggcttatagg gatctgggct gagcacaaag tcaaatgcct tgtttaaggg aaacgagtag
     4441 ctccttaaac aacggctaca acttccttca agcttcccgc caaacgagcc tttgaagaaa
     4501 atctcttgac cggaccgata atagactaaa tcgacctcca ggaacggcgg aaagccaaaa
     4561 tcccgaaact ggccattttt atagagatcg ttcagtccat caattcgttc ggcaaagcta
     4621 atctctttgg cggattcggt aatctcgtcg acgggaattt tcatcggcgg acaagcaagc
     4681 tgttcggtag aatatagtca atcaaatttt attgtcaagc caagctccgt ccatcagatc
     4741 aaaaagcgcg tgatttatta aacacttcca aagtgtcact atgactaggc acttgaccca
     4801 atggcgttaa cgtagaagcc tggcatagac ctcctttgag gcgtggcagc atgaggtaaa
     4861 tctcttgagt attgagcggc tttttgttaa ccaagttcag ttcacgttat gagctttccc
     4921 gtcacactac gcaagcaatg gcatggcagg cgctacaatt cttataaccg cttgctgaaa
     4981 gatacttttg atgcacgggt ctacaagatt ggtctcaggc tggacttcac ctgtccgaat
     5041 cgggatggaa cggttgccgt aggaggatgc atctactgca acaattctag tcatacgcca
     5101 cagaactatg agcctcgcac atcggtaacg gaacaacttg agcgcggggc cacggcgatt
     5161 caacgacgtc atcgagccga gaaattcatc gcctactttc agagttacac aaatacctac
     5221 ggttcggtct ctaagctcga aaaactttat cgcgaagcgc tagattttcg tggagtcgtc
     5281 ggtttgtcca tcgccacccg gcccgattgt ctcccagagg ataccctgga cctcctaacg
     5341 gacctctccc atcataccta tctttggttg gagatggggc ttgagtcgat gcaagaccgg
     5401 acgctccgat gggtaaaccg cggtcatgga ctaaacgaat tcgtcgaaac cgcccagcgt
     5461 tgcaagaaac gaggactccg gctttgtacc catttgatcc tcggtttccc tggagaaagc
     5521 cgtgccgaaa ttcttgcgac aacggctttg ttgaatcggc tgcagatcga tggtctaaaa
     5581 ctgcacaatc ttcatatcat caagaatacg gtcctggaaa aactttatgc cacgggtggc
     5641 ttcaccctct tcagccagaa cgaatatgtg acactcgtgg ttgatttcct cgagcgtttg
     5701 gcgccggaaa tggtt
//
LOCUS       DASSWZ010000148         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_953016, whole genome shotgun sequence.
ACCESSION   DASSWZ010000148 DASSWZ010000000
VERSION     DASSWZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953016"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..1080
                     /locus_tag="VFN58_03665"
     CDS             469..1080
                     /locus_tag="VFN58_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917120.1"
                     /translation="MFSHNPLIRRGALEEKELLDKLFRILFKVIHEIPTSTEKETSDA
                     RIRSRKLISEASFKAAAISGALALPSGPLAWFTILPDLAAIWRLQAQMVADIGAVFGK
                     KGKLTEESMIYCLFRHATAQVVRDLVTRMGERVVVQSASLRMAENVLQGVGIRLVHRV
                     ARRGLWRLLPAIGALAVAGYAYYDTEQVGQTAIEFFSKEIELK"
     gene            1311..1925
                     /locus_tag="VFN58_03670"
     CDS             1311..1925
                     /locus_tag="VFN58_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8917121.1"
                     /translation="MKQLRRVLFFVSVTLLTGIGCLKFDSEGDDSIGAAYAEEQDRDT
                     TYIAPFVPTPQEVVDRMLELAQVSQEDILYDLGSGDGRIVITAARRYRAKAVGFEIDP
                     ALVTYSQRNIKEAGVEALAEIREQDIRTVDLSPASVVTMYLYPGANLRLRAAIMRQLK
                     PGSRVVSHDFSMGDWKPDRVERMTDSGGFLRTIYLWRIGDRSTR"
     gene            2521..>2942
                     /locus_tag="VFN58_03675"
     CDS             2521..>2942
                     /locus_tag="VFN58_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917122.1"
                     /translation="MNTAHRSLLSQGIPVTNSEVASAHLARGEIAEADEEWGLALEHY
                     GIGLSLLPEDTRTVYFLFNNAAHCLNALELYSEGESYCRRAIEIDSMRPGAYRNLAVS
                     LRGLGNLRGSALCLVKAIKISPSDERAVQFLKQLLTDHP"
BASE COUNT          687 a          796 c          765 g          694 t
ORIGIN      
        1 gacctacgcc gacagctcca tcgccgagcg gactgttggt gtcacggttt caaatacgta
       61 accgccacaa ggccccgtcg cataggccgc tcttaattaa ttgtttgtgc aaaccgggcc
      121 gtgaagcgac gagaaagcaa actctctcca tcggtttctc cccgccaggc gcggataagc
      181 ccaagggtgc ccaggaaaaa aaccagatcc tcccgaccca aggccgctct ttttccgtcc
      241 ttattacttt cgctagctga cgttggcatt tttttcttgc catcattata cttcttcgat
      301 actatttgac tgtgcgctgg ccgcaccgcg aggcattaaa tcttaccatg atgtgcgagc
      361 gtgaccgaaa atcacctgac accagagttt ttccaagctg actggtcgtt gacaacagat
      421 cgcacacaaa cgttttaagt ctccaagaac tcatgtgtgg aggaccgcat gtttagtcac
      481 aatccgctta tccgcagagg tgcgttagaa gaaaaagaat tgctggacaa actatttcgc
      541 attctcttta aagtgatcca tgaaattccg accagcactg agaaggaaac gagcgacgcc
      601 cggatccgtt ctcgtaagct catctctgaa gcctccttca aggccgctgc gatctccgga
      661 gcgctcgcgt tgccgtccgg gcccctagca tggttcacga ttctccctga tctggcggcc
      721 atctggcgac tgcaggcgca aatggtggcc gacatcggcg ccgtgttcgg caaaaaagga
      781 aagctcacgg aagaatcgat gatttactgc ttgttccggc acgctaccgc ccaggtcgtg
      841 cgcgacctcg tgacacgcat gggcgagaga gtcgtcgtac agagcgcttc gttacgcatg
      901 gccgaaaacg tgctgcaggg cgtcggaatt aggctcgtcc accgcgtcgc gcgacgcggg
      961 ctctggcgac tcctgcccgc catcggcgcc cttgccgtcg ccggctacgc ctactacgac
     1021 actgaacagg tcggccaaac cgccatcgag ttttttagca aggagatcga gttgaagtag
     1081 ttgtgctcac tctcattctt atccttttgg ctctgcggtt tggtttttta caacaattga
     1141 cagctgacgc gtgagcgaca cagaatctgc caatgcttcg agggatgaaa cctggggcat
     1201 ctcattccac tttttcctat gtctcagcgt tcttcataac ctgcagaatt cctttccttg
     1261 agcagtgagc tggtacacta ggacgcttct ggatagcgga cgatgatcag atgaaacagt
     1321 tacgtcgggt tttatttttt gtctccgtaa ctctactcac gggaatcggc tgcctgaaat
     1381 ttgactccga aggcgacgat tcgattggcg ccgcttatgc ggaagaacag gaccgcgaca
     1441 cgacgtatat cgctccgttc gtgcccacac cgcaagaagt ggtcgatcga atgctggagc
     1501 tggctcaagt cagccaggag gacatccttt atgatctcgg atcgggagac ggaagaatcg
     1561 tgattacagc agctagacga taccgggcaa aggccgtcgg ttttgagatc gatcctgctc
     1621 tggtgacata ttcacagcgc aatatcaaag aagccggtgt ggaggctctt gcagaaattc
     1681 gcgaacagga tatccgaacc gtggacctat cgcctgccag cgtcgtgacg atgtatctgt
     1741 atcccggagc caacctgcga ttgagagctg cgatcatgcg ccagttgaag ccgggttcaa
     1801 gagtggtttc tcacgatttc agcatggggg attggaaacc tgatcgggtg gagcggatga
     1861 cagactcggg tggcttcttg cgaacgatct atctctggcg cattggagat cggagcacgc
     1921 gctgagatga ttgtcctcgc accttgaccg ctggagcgag cagcaactga aggtcgtgtt
     1981 gaccagccct catctatgcg ccacagtagg attagaaaag ggggaccgcc agtgcctcaa
     2041 tacgacattt acgtgcgctg cgttgaatgt gctgccgtgc atcccatgcg cattcgcatt
     2101 catctggaca acggtccggt ggagaaacag agtatcgccg aaacttacca aggaagaacg
     2161 cggcctccac agcttcaagc tatcgaaggc cacaaaacct tatgtctaaa gacaggacgg
     2221 atgttcatcc aagaaaacca taatcaaata tttttggtcc ctagctacag ctccgaacgc
     2281 atcacctaga ttcgagccgc tgcaacaaac tatcggccac cagacggtga tggttcagat
     2341 cgaaaaataa aatagcaatc agcggagtgc gaaagcggga gtatccccga ctgtgccatg
     2401 agctgacatt ttgacacaat gagctgtcaa attacctgcc gtggcctcca cagactacat
     2461 atttctaggg atttctagcc ttttctttcg tggcgtgtcc tttgctcttt tttttagggc
     2521 atgaacacag cccacagatc tctcctatcc caaggtatcc ccgttacgaa ttcggaagta
     2581 gccagcgccc atctcgcgcg gggtgaaata gctgaggccg acgaggaatg gggattggct
     2641 cttgagcatt acggaatcgg cctatcgctt ttgccggaag acacccgaac ggtgtatttc
     2701 cttttcaaca atgccgccca ttgcctcaac gcgctggagc tttactccga gggagagagc
     2761 tactgccgtc gtgctatcga aatagattcg atgcgacctg gagcttacag gaatttagcc
     2821 gtttccctcc gcggcctggg taacttgaga ggctctgcgt tatgtttggt caaagccatc
     2881 aaaattagcc cttccgatga gcgcgctgtg cagtttctca agcagctgtt gacggatcat
     2941 cc
//
LOCUS       DASSWZ010000149         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1306616, whole genome shotgun sequence.
ACCESSION   DASSWZ010000149 DASSWZ010000000
VERSION     DASSWZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306616"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFN58_03680"
     CDS             <1..219
                     /locus_tag="VFN58_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HET8917123.1"
                     /translation="ERRAMAVALEQEMRARVVEAEAEVPRAMAEAFRQGNLGIMDYYK
                     MKNVQADTAMRDAIGDTGDGHRAATPKQ"
     gene            231..575
                     /locus_tag="VFN58_03685"
     CDS             231..575
                     /locus_tag="VFN58_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917124.1"
                     /translation="MRNLGIGNVLLLLFFVVLPLMNFLLRRFRKRRHHPIPQEKPVEQ
                     MLRQAQTTPMPRPTPRAAGDRVHGSPARAVTPLANDRVTRRSLLGTTRDARRGIVIMT
                     LLGPCRAFDPPD"
     gene            1158..1916
                     /locus_tag="VFN58_03690"
     CDS             1158..1916
                     /locus_tag="VFN58_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8917125.1"
                     /translation="MAAVFAHEAGNPLAGILLSLKCVESQLEKQEVNDPSLISTIRGA
                     IGEIGRLGSLLKEFRSLVPEQTLDLKCTDLVKIAEEALALEMIAYRASGITVKFAFED
                     ALPLVRLDTAKIKQVILNLCRNAVEAMPDGGCLTLKGYHSRGMVVLEVSDDGIGVPDD
                     INVFELFKTTKPGGTGLGLPLVQQIVSAHDGTIAYKSKPGRGATFKVSLPLSENNAMA
                     GSAALMKGQMMQPYKELRRLGFDIKKQQKLPALS"
     gene            2096..2776
                     /locus_tag="VFN58_03695"
     CDS             2096..2776
                     /locus_tag="VFN58_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET8917126.1"
                     /translation="MIQQTPQADLFKQQLRDSLQRETLNARAVKIATHTNVYTCGDQD
                     EMVYFIESGQIKLLMLSSEGKECLLAIHTAGDIFGELCLSRLGARLETATAMKQTVLK
                     QIPSSQFFARLNRDSLIEGFVQYLAVRIADQQQVIANLVTVDSEQRLGKTLLQLARTL
                     GKKDPRSIRIELKISHEELSEMVGTTRPRISMFMQRFRNLGLIEMSTEHFLIIKENKL
                     TAYLAQIA"
     gene            2931..>3626
                     /locus_tag="VFN58_03700"
     CDS             2931..>3626
                     /locus_tag="VFN58_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET8917127.1"
                     /translation="MLSPLTSEHASNTEPTPQPRFKKFRIVLIKPSKYDDDGYVIRFW
                     KGVLPSNTLNVLHGLTEAVKDSGVFGDLKIEIVTFDETAESLPLRRIIGWSRRSSTKL
                     VVGLVGVQTNQFPRAFDLARQFRAAGIDVIIGGFHTSGTINMLSDQEPDIQALVKESI
                     TIVSGEVEENWAEILADALHGRLKPIYSFAQDLQNLVDIGNAALPRISPKTMRHFAKP
                     SFGTADTSRGCPFA"
BASE COUNT          904 a          957 c          977 g          788 t
ORIGIN      
        1 gagcggcgcg ccatggcggt tgctttggag caggaaatgc gcgcccgcgt cgtcgaggcg
       61 gaagcggaag tgccgcgcgc catggccgag gcgtttcgcc agggaaacct ggggatcatg
      121 gactactata aaatgaaaaa tgtgcaggcc gatacggcca tgcgcgatgc gatcggtgat
      181 accggagacg gccatcgggc cgcgacgccg aaacaataag cgaggccgct atgagaaacc
      241 tggggattgg gaatgtcctt ctgctgcttt ttttcgtcgt gctgcccctg atgaacttcc
      301 tgctgcggcg cttcaggaaa cgtcgtcatc atccaattcc acaggagaag cctgtggagc
      361 agatgctccg tcaggcgcaa acgaccccga tgccccggcc aacacctcgt gccgccggcg
      421 accgagtgca tggatcgccg gcgcgcgccg ttaccccgct tgctaacgat cgtgttacca
      481 gaagatcgct tttaggaacc acccgcgatg cgcggcgtgg catcgttatc atgacgctcc
      541 tgggaccctg tcgcgccttc gatccgccgg actagtgcat cccctcatcc tttttgcctc
      601 ctccatacac gactggacgt tctcgtctgt catcgcctct gctgttgttc aatcaggatg
      661 ggtggttctg aggataggac gacgatgcgc gtacaagagt gatccggagc cgtagcgggg
      721 gtccgcgggc ctcaggttag tttctttcaa aattgtccaa tattagacag aaaccccttc
      781 tcattcggcg tatgttaaac cccgcatgtg cgctaactaa tgggtgaaac cctgcttagg
      841 gacagcgggg gtgtatatcg atcaccttta ttttgatcta gatcaacgag atgcattccg
      901 ggtcgccggc aaaagatccc tgctttcgtt ggcgctgaga aatcaaaaaa catattccaa
      961 gagaggagaa tctcatcgaa cgaacatacg agaaaactaa agtcggccgc ctctgcaaga
     1021 aaagctttgc tgaaggcacc aaaaatccta taagaagtaa agcccggctc ggtttgaaac
     1081 tgtgtgaaag ccgccgcgac caggaccggt cggaacaacg gccgcaagag accgaacgac
     1141 tcgctttatt gggaactatg gctgccgtct ttgcccatga agctggcaac cccctcgccg
     1201 gaatcctctt gtctctaaag tgtgtggaga gtcagctcga aaaacaggag gtcaatgatc
     1261 cctctttgat atcgacgatc cgaggtgcga taggagaaat cggtcgtcta ggctcattgc
     1321 tcaaagagtt tcgctctctt gtgcccgagc aaactctcga cctcaagtgc accgatcttg
     1381 tgaagatcgc cgaagaggcc ttggctttag agatgatcgc ctatcgggct tctggaatta
     1441 ccgttaaatt cgccttcgag gatgctttac ccctggttag gttggacaca gcgaaaataa
     1501 aacaggtgat cctcaatctt tgcaggaatg cggttgaggc catgccggac ggaggatgct
     1561 taacgctcaa aggctatcac tctcgaggga tggtagtctt ggaggtcagt gatgacggta
     1621 ttggagtccc tgacgatata aacgttttcg agttattcaa gaccaccaag ccgggcggca
     1681 ctggtttagg acttcctctc gttcagcaga ttgtctcagc gcatgacggc acgatcgcct
     1741 acaagagtaa gccgggccgc ggggcgacgt ttaaagtctc tttgcccctg tctgagaaca
     1801 acgcgatggc tgggtctgca gctctgatga aagggcagat gatgcaaccc tataaagaac
     1861 tccggaggtt gggcttcgac atcaagaagc aacagaagtt accagccctc agttagctag
     1921 tggacttagg cattagaacc aagcgagtct ggacataccg ccgagaaaga catacagtca
     1981 tatggagacc tggcaaacga agtatggcgc ctgaaaagaa agctcactgg ccagctccct
     2041 gaggaaatcg actatcgttg tggtcatcat cgtgggcttc aaaggaggcg cgattatgat
     2101 tcaacaaact cctcaagcag acctgttcaa acaacaattg cgtgattcgt tgcagcgcga
     2161 gactttaaat gctcgcgccg tcaagattgc cacacacact aacgtctata cctgtggcga
     2221 tcaagacgaa atggtctact tcatcgaaag cgggcaaatc aaactgctca tgctgtcgtc
     2281 cgaaggtaag gagtgcctcc tggccatcca taccgccgga gacatttttg gggagctgtg
     2341 tctatccagg ttaggcgcgc ggctggaaac agcaaccgcg atgaagcaaa cggtcctaaa
     2401 acaaattcct agttctcagt tctttgcacg tttaaaccgc gactcgctaa tcgaagggtt
     2461 tgtgcaatac ctggcagtgc gcattgccga ccagcagcag gtgatcgcca atctggtgac
     2521 cgtggatagc gaacagcggc tcggcaagac cttattgcag ctggctcgca cattgggcaa
     2581 gaaagaccca cgtagcattc gcatcgaact gaagatttcc catgaggagt tgtccgagat
     2641 ggtgggaacg acgaggcccc ggatcagcat gtttatgcag cgatttcgaa accttgggct
     2701 gattgagatg agtacagagc actttctcat catcaaagaa aacaagctca ccgcgtatct
     2761 cgcacagatt gcctgagcgg ctgcggccgc cataattctt ttcaaaatcg tctaatatta
     2821 gacagcaacc caggcggact cgccgtatca tcgtcgggca cccgttgggt gtgaaaacaa
     2881 tcagacatta aagaatgcat attattccat cttaggaagg cagaaaaaat atgttaagcc
     2941 cactgaccag tgaacatgcg tcgaacacgg agccaacccc acagccgcgg tttaaaaagt
     3001 tccgcatcgt tctgatcaaa ccctccaagt acgacgacga cgggtatgtc attcggtttt
     3061 ggaaaggcgt gctgccgagc aacacgctca acgtcctgca tgggctgacc gaagcagtga
     3121 aagacagtgg tgtattcgga gaccttaaga tagaaatcgt gacctttgac gagactgcag
     3181 aaagccttcc gttaagaaga atcattggtt ggagccggcg ttcttctacg aaactcgtgg
     3241 tgggattagt gggggtacaa accaatcagt ttccacgagc gttcgatctg gcccggcagt
     3301 ttcgcgctgc cggaatcgat gtcattatcg gcggctttca caccagcggc acgatcaaca
     3361 tgctgagcga tcaggagccc gacatccagg ctttggtgaa agaatccatc accatagtat
     3421 cgggagaagt cgaagagaac tgggcggaga ttctggccga tgcgctccac ggccgactaa
     3481 agccgattta ctcattcgcc caagacctgc aaaacctcgt cgatatcggc aacgccgcgc
     3541 tcccccggat cagccccaag accatgcgcc actttgctaa gccctcgttc ggaaccgctg
     3601 acacttcccg gggttgtcct tttgcg
//
LOCUS       DASSWZ010000150         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_684138, whole genome shotgun sequence.
ACCESSION   DASSWZ010000150 DASSWZ010000000
VERSION     DASSWZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684138"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFN58_03705"
     CDS             complement(<1..234)
                     /locus_tag="VFN58_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917128.1"
                     /translation="MQSQSTGFRIIGFTAAVCLFAIATRGGAQTKFERLHVGYSAQAG
                     SLAPIWITKEAGIFKKHGLDVELLFIPGGPTAAA"
     gene            complement(393..1385)
                     /locus_tag="VFN58_03710"
     CDS             complement(393..1385)
                     /locus_tag="VFN58_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917129.1"
                     /translation="MIKTRCFPALLVTAIGLMLCGTENTFSAEGLGNLTRVRMGLAAR
                     STTSMPFFVAKERGFFREEGLDVELIVMQAIQTIQATMGNSTQFASATGSAISAAVSG
                     ADIRVILAVTDQPSFDLIVQPNITSVQQLRGKKIGTGGVGSLAEILARRILIAHNVRP
                     EDVTILATGPSHMTYLSLKAKVIDAAPLQMPLTFTAQDEGFRKLVSAADVYRAVQGGL
                     ATTKAMLTEQPELVTKVVRAMLKAIRLIKSDRKFSIDFIKGPWLDIGKEPEKIAARVY
                     DIAGPALLENGWVSEEMQRQMIADAAVRIKPKQPVLPEQVFDFSIVRKVGETLR"
     gene            1596..>2363
                     /locus_tag="VFN58_03715"
     CDS             1596..>2363
                     /locus_tag="VFN58_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8917130.1"
                     /translation="MTTTYDAIIIGTGQAGPFLAGRLTTAGMRVAIIERKLFGGTCVN
                     TGCIPTKTMVASAYAAHMASRANDFGVNTSGVSVDMKRVKARKDEVSGQSRTGLEGWL
                     KKMENCTVYEGHARFQSPREVAVGSERLTAERIFINVGGRAATPEMQGLKQIEYFNNS
                     SMMNVDFLPEHLIVVGGSYIGLEFGQMYRRFGSKVTIIEMAEGLIHREDQDVSSAIKE
                     ILEREDINIRLNAKCIGFSKRGKDIITHVECTTGAPDV"
BASE COUNT          560 a          635 c          623 g          545 t
ORIGIN      
        1 ggctgccgcc gtcggaccac ccggaataaa aagcaattca acatcaaggc catgtttttt
       61 gaatatgccg gcttcctttg tgatccatat cggtgcgagg gaaccagcct gggcgctgta
      121 gccgacgtgc agtctttcga atttcgtctg cgcaccaccc cgcgtagcga tcgcaaataa
      181 gcaaacggcg gcagtaaacc ctatgattcg gaaacccgtt gactgcgatt gcatgcgcaa
      241 gttcctccat acaaacactt ctcgccagcc ttaacgcctt gcatcatcat tagttagttg
      301 cgaaatcggc ggttctttta atttacgagc cggtttaatg tgcatcaggg catctctttc
      361 gctcacaagg tcgcagaacg ttagcgagaa cactatctca acgtctcgcc gaccttgcgg
      421 acaatgctaa agtcgaatac ctgctcggga agcaccggct gcttgggttt aattcgaacc
      481 gcggcatcgg cgatcatttg gcgctgcatc tcttcgctca cccagccatt ttccaaaagc
      541 gccggccccg cgatgtcata cacccgggcc gcgatctttt ccggttcctt gccgatgtct
      601 agccacgggc ctttgatgaa atcaatgcta aacttgcggt cggatttgat caggcgaatc
      661 gctttcagca tcgcgcgcac cactttggtt accaactcgg gctgctcggt cagcatcgct
      721 ttggtcgtcg cgagaccgcc ctgcacggct cgataaacgt cggccgccga caccagcttg
      781 cgaaaccctt catcttgagc ggtgaaggtt aagggcattt gcagcggcgc ggcatcaatc
      841 actttagcct tcaaggataa atacgtcata tgactcggac ctgttgcgag aatcgtcaca
      901 tcttcgggac gcacattgtg cgcgatcaga atccggcgtg cgagaatctc cgctaaagag
      961 ccgaccccgc cggtgccgat tttttttccg cgtagctgtt gaacgctggt aatgttcggc
     1021 tggacgatca agtcgaatga cggttgatcc gtgacggcca gaatcactcg gatatcggcg
     1081 ccgctaacgg cggcactaat ggcggagccg gttgcagaag cgaactgagt gctatttccc
     1141 atcgtcgcct gaatcgtctg gatcgcctgc attacgatca actccacgtc caagccttcc
     1201 tcacgaaaaa agccacgttc tttggcaaca aagaaaggca tagaagtcgt gctcctggcc
     1261 gctagaccca tgcgaacccg ggtcaggttg cccaagccct ctgccgagaa agtgttttcc
     1321 gttccgcata gcatcagacc gatcgcagtc accaaaagag ccggaaaaca tctcgtctta
     1381 atcattgcgt ccctcctcat gatccagttg aaacttaacc ggcagaatct gaagaatccg
     1441 tcgatctatg cggcattaag ccaatttcta ttctcctgtc aacagacgat tgagaaccgt
     1501 tgtgttcgga ttgaatcggc gataggaaat acagttagga acgttgcttg taagatcgat
     1561 caggctagat gtgacgcagg ttaagtgagg cttttatgac taccacgtac gatgcgatca
     1621 ttattggcac cggccaggct ggacctttct tggcgggacg cctaacaacc gccggaatga
     1681 gagttgcgat catcgaacgt aaattgttcg gtggcacctg tgtcaacaca ggttgtattc
     1741 ctaccaagac catggtcgcc agtgcttacg ccgcccacat ggcaagtcgg gccaacgact
     1801 ttggtgtgaa cactagcggc gtaagtgtcg acatgaagcg tgtaaaggct cggaaggacg
     1861 aagtctcagg acaatcgagg actggtctcg agggctggct gaagaagatg gaaaactgca
     1921 cggtctatga gggccacgcc cgcttccagt cgcctcggga ggtagcggta ggttcggaga
     1981 ggctcacggc tgaacggatc ttcattaacg tcggtggacg cgcggccacc cctgagatgc
     2041 agggactgaa acaaatcgaa tattttaaca acagttccat gatgaacgtc gatttccttc
     2101 ccgagcacct gatcgttgtg ggaggcagtt acatcggtct ggaatttggg cagatgtacc
     2161 gccgttttgg cagtaaagta actatcatcg agatggctga gggattgatt caccgtgaag
     2221 accaagacgt atcgtcggct atcaaggaga ttctcgaacg cgaagacatc aacatccgat
     2281 tgaacgcgaa gtgcatcggc ttttccaagc gaggcaagga cattattacg cacgtggagt
     2341 gcaccactgg cgctcctgat gtt
//
LOCUS       DASSWZ010000151         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_188933, whole genome shotgun sequence.
ACCESSION   DASSWZ010000151 DASSWZ010000000
VERSION     DASSWZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188933"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFN58_03720"
     CDS             complement(<1..626)
                     /locus_tag="VFN58_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917131.1"
                     /translation="MSSEAETLQKKLAELLSWERRKRREQILATLFCYALLAALLTLP
                     FHGFLETVISRWFIPILFFGLMAPFFLGRGRWQLADSARALARVDKTLRLDERALTAW
                     ELLERNETRAAALMVLKQAGEKLTNLDPKALFRRSWSWQAYCALPLLLLWLGLVWFDV
                     GLRFDSGVQLSAPKTVAQKLREFSRELQEKAKNEGLHESFRVGRELEQV"
     gene            complement(623..1369)
                     /locus_tag="VFN58_03725"
     CDS             complement(623..1369)
                     /locus_tag="VFN58_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917132.1"
                     /translation="MKNNPVLRESLDIFFGEGHGFAVYFYLLIILAPVEFFSLYLPSL
                     DAQMWSGSANLFKVCAVTALLLTVYLALRVANQEFAPWRFKPVKRWVRESGLAISAVG
                     QGQLAFLSLHVVLSILLCTPLLIWAGAIARTPFPRVAGTFLLLLFYALSYGVWGLVTL
                     ALWERRAESRQVFIRCFFFFLVIFSALFYLPVNPVAFLLAYIGRQELPPLSMGGWKAP
                     ATTVHFTFHLLIGSIGLAAHRWALRKDLTV"
     gene            complement(1366..3345)
                     /locus_tag="VFN58_03730"
     CDS             complement(1366..3345)
                     /locus_tag="VFN58_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917133.1"
                     /translation="MGLHSLWTLWRKFTGGKRLTHRPAWSSLLLFSFFLFPSLCGAEI
                     FIEPRIGFHGVFQLGRPFPLEIELSNSGRPAEGILEITVWKGGATKGGAPYPLYYRRD
                     VFLSPQSRKTVQLTVDPDFISRPLKIVFSSPAGTASREIDLRRHFSPSPVILLVSEGN
                     TIPAVSLSSTLTSRLVALTLAELPPDPRALLGVSHLILYDQSLRDLSRSQLLAFDTWL
                     TAGGRMVILGSLNYSLYQEPTISRFLPVRVTGTKRISFHPALGQGERASPIADVWAQA
                     STLIGGKVLAEAQGMPVLVETSRGKGRITYLSLDVGRPPLSQWDGLPRFLQNLLAPAG
                     GEELAPRTRWDDSVFSQLILSPSFISTYLPTGSLFVAIVGYLIGIGLFSWVWQRQAFT
                     RRALVAAFLFFVVLSTCGGYLLFSRGGNIPDGVLLSSSVMESSADGYVEVQSNLAMFS
                     TQIRQYGLQMERGWMELVPVAAPSRDRPEPTVVFQDGSGTSRFQLPLREWDYRLFRIR
                     FVDRFPLRAEFEQQAGKLRMRLNNQSAKDLADCWLVVPGQRYALGDIPRGASWTKEFP
                     LASDENQERSGGRRSDAVDFREISFKDKTQEILFHSSFFPRDEGSVRWSTGAAVFFGW
                     VKDPDRRVSVDDQRIRNYDYTLFRTIFPLAGPEDE"
     gene            complement(3456..>3860)
                     /gene="greA"
                     /locus_tag="VFN58_03735"
     CDS             complement(3456..>3860)
                     /gene="greA"
                     /locus_tag="VFN58_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET8917134.1"
                     /translation="ERPKIVREIEEARDHGDLSENAEFHAAKERQSLLDVQIREIEDR
                     LARAQVIEVSKLSGDKVVFGATVSLADGDTGDKVVYQIVGDHEAEPKNGKISISSPVA
                     RALIGKSEGDEVQVHTPTGVRSFEILSVEYID"
BASE COUNT          974 a         1125 c         1040 g          721 t
ORIGIN      
        1 acctgctcca actctcgacc cactcggaag ctctcatgta gaccctcgtt cttcgctttc
       61 tcctggagtt ctcgagaaaa ctctcgcagc ttctgcgcca cggtcttcgg agcagaaagc
      121 tgcacaccgc tgtcgaagcg aagaccgacg tcaaaccaca caagtccgag ccaaaggaga
      181 agaagcggaa gagcgcaata ggcctgccag ctccagcttc tgcgaaacaa tgccttagga
      241 tcgaggttcg tgagtttctc ccccgcctgt tttaaaacca tgagcgccgc cgctcgcgtc
      301 tcattgcgct ccaacaactc ccacgcagtt agggcgcgct catcgagccg cagcgtcttg
      361 tcaacgcgcg ccagcgcccg cgcggaatct gccagctgcc aacgccccct gccgaggaaa
      421 aacggcgcca ttaatccgaa aaataaaatt ggaatgaacc agcggctgat caccgtctcc
      481 aagaagccat gaaacgggag ggtgagtagc gcggctagca atgcatagca aaaaagtgtg
      541 gcgaggatct gctcgcgccg ttttctccgt tcccagctca gcagctcggc gagtttcttt
      601 tggagggttt ctgcttcgga actcaaacgg ttaaatcctt cctcagcgcc cagcggtgcg
      661 ctgctaaacc gatgctgcca attagcaggt gaaatgtaaa atggacggtg gtcgcaggtg
      721 ccttccatcc acccatactg agcggtggca attcctgtcg gccgatatag gcgagcagaa
      781 aggccactgg attaaccggc aaatagaata gggctgagaa gataaccagg aaaaaaaaga
      841 agcagcggat gaaaacctgg cggctctcgg cccgccgttc ccataaagcc aacgttacca
      901 gtccccagac cccataactg agagcataaa ataacagcag caagaaagtg ccggctaccc
      961 gcgggaatgg agtccgagcg atagctcccg cccagatcag gagcggcgtg cagagaagaa
     1021 ttgaaaggac cacatgcaaa gacagaaacg ccaactggcc ttgacctacc gcggagatcg
     1081 ccagcccgct ctcgcgcacc caacgtttaa ccggcttgaa cctccaggga gcgaattcct
     1141 gattggcgac gcgtaaagca agataaaccg tcagtaacaa cgcggtcacc gcacaaacct
     1201 taaagagatt cgcggaaccg ctccacatct gcgcgtccaa tgacggcaaa tagagcgaaa
     1261 agaactccac cggcgccagt ataatcaaaa gataaaagta gacggcaaac ccatggccct
     1321 cgccgaagaa gatatcgaga ctctcgcgca gaaccggatt attcttcatt catcctccgg
     1381 acccgccagt ggaaaaatcg tgcgaaacaa cgtgtagtcg tagttccgaa ttctctggtc
     1441 gtccaccgac acgcgccggt ctgggtcttt cacccaacca aagaacacag ccgcccccgt
     1501 gctccaacgc accgagcctt cgtcccgggg aaaaaacgag gaatgaaata agatttcctg
     1561 ggttttgtct ttaaaggaaa tctcgcggaa gtctaccgca tcagaccgcc ggccgccgct
     1621 tcgctcttgg ttttcgtcgc tggcgagagg aaactctttc gtccaactcg cgccccgggg
     1681 gatatcgccg agagcatagc gctgacccgg caccaccaac cagcaatccg caagatcttt
     1741 ggcgctctgg ttgttcaatc gcatgcgtag ctttccggct tgctgttcaa actcggcgcg
     1801 tagcgggaaa cggtcaacaa aacggatacg aaaaagtcta taatcccact cccgtaacgg
     1861 caactgaaaa cggctggtgc cgctgccgtc ctggaaaacc accgtcggtt cgggtcgatc
     1921 cctggaaggc gcggcaaccg ggactaactc catccagccc cgttccatct ggaggccata
     1981 ctggcggatc tgggtggaaa acattgccag gttcgactgg acctcaacgt aaccatccgc
     2041 gctgctttcc ataacgctgg aagaaagcag tacaccgtcc gggatattac cgccgcggct
     2101 gaataagaga tagcccccgc aagttgaaag aacgacaaag aacaggaaag cggccaccaa
     2161 agcgcgtctg gtaaatgcct ggcgttgcca aacccatgag aaaaggccga taccgattag
     2221 gtaaccgact atggccacga acagagagcc ggtgggaagg taagtggaga tgaacgacgg
     2281 gctgagaatg agctgagaga acactgagtc gtcccaccgt gtccgtggcg caagctcttc
     2341 gccgccggca ggcgccaaga ggttttgtag aaacctcggc agcccgtccc actgggagag
     2401 cggcggccgc ccgacatcga gggacaaata ggtaatccga ccttttcctc ggctggtttc
     2461 cacgagcacc ggcatgccct gtgcttccgc cagcaccttt cccccaatca acgtcgatgc
     2521 ctgcgcccag acatcggcaa tgggcgatgc tcgttcgccc tgacctaaag caggatgaaa
     2581 cgaaattctt ttggttcccg tgacacgcac cggcagaaac cggctgatcg tcggctcctg
     2641 gtagagggag taattgaggc tgccgagaat caccattctt ccgcccgccg tcagccaggt
     2701 gtcgaaggca aggagttgcg atcgagaaag atcgcgcaac gattggtcgt agaggatcaa
     2761 atgcgagaca ccgagcaacg ctctcggatc aggcggcaac tcggccaatg tgagggcaac
     2821 caaacgactc gtcaaggtgg agctcagaga aaccgctgga atcgtattgc cttcgctcac
     2881 cagcagaata accggggagg gtgaaaagtg acgccgcaga tcgatctcgc gcgatgccgt
     2941 gccggcgggg cttgaaaaca caatttttaa aggccggctg atgaaatccg ggtcgacggt
     3001 cagctgcacg gtttttcggg attggggaga gagaaaaaca tctctgcggt aatataaagg
     3061 atacggagca ccgcctttcg ttgctccccc tttccaaact gtaatttcta aaattccttc
     3121 ggccggccgg cccgagttgc tgagttcgat ttccagtggg aaagggcggc cgagttggaa
     3181 aactccgtgg aagccgatgc gcggctcgat aaatatttca gcgccgcaaa gggacggaaa
     3241 aagaaaaaag ctaaagagca gtaaagacga ccaagccggt cggtgagtga ggcgtttccc
     3301 gccggtgaat ttccgccaga gagtccaaag agaatgtaaa cccacaccag cgcgcaagct
     3361 taatgggggg attggagaag catcgcgacc tggcgtgtcg actgaaaagg gcatgattcc
     3421 tttcccccgt caggcccgcg ccaccgctga gagatctagt cgatgtactc tacactcaga
     3481 atctcaaatg aacggacgcc cgtaggggtg tggacctgaa cctcgtctcc ctcgctcttg
     3541 ccgatcaatg cacgggcgac gggagagcta atagaaatct ttccgttctt cggctctgcc
     3601 tcatgatcac ctacgatttg atataccacc ttgtcgccgg tatcaccatc ggccaaggat
     3661 accgtagcgc cgaataccac tttgtccccc gagagcttgg agacctcgat cacctgcgcg
     3721 cgcgccagcc gatcttcgat ctcgcggatc tggacatcca acagcgattg cctttccttg
     3781 gccgcgtgaa actcggcatt ctcggacaga tcgccgtggt ctcgggcctc ttcaatttcg
     3841 cgaacgattt tcggcctttc
//
LOCUS       DASSWZ010000152         5603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_75694, whole genome shotgun sequence.
ACCESSION   DASSWZ010000152 DASSWZ010000000
VERSION     DASSWZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75694"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFN58_03740"
     CDS             complement(<1..382)
                     /locus_tag="VFN58_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET8917135.1"
                     /translation="MAFFQYRAADQAGKVVEGVMEADAEQGVVTRLHEMGCIPIRIAM
                     PGDRLIAGSQTRLPLFPKRKVNQQQLLQFTQELSTLLAAGLPLDRSLSLLANLIEGED
                     FIKVMRTLLEAVRAGKSLAASMGEH"
     gene            complement(463..2163)
                     /gene="gspE"
                     /locus_tag="VFN58_03745"
     CDS             complement(463..2163)
                     /gene="gspE"
                     /locus_tag="VFN58_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HET8917136.1"
                     /translation="MDSHLSLTEQLIERRLLSSEEMERVAKLQQEQQAPLTRLIVELG
                     FLSEDDLLPVLRDHFEIPMIALKDFPVTPLPVEFSPTLGDFFKQARMVPIKIEGRDLV
                     VAITDPLDLSRLHALELAAGLHVKPVLAKEKDIMARIEALFGNSYSTEANTGSAAREI
                     EGAVDEEDVAHLRDMASEVPVIRLVNQMLVRALESRASDVHIEPFENQLKVRYRIDGI
                     LHEVEPPPRQLKAAVISRLKILAQLNIAERRLPQDGRIKIRLAGKDVDLRIATVPTLY
                     GESVVIRLLERAQIFTQMDTLGFPTTILQQFNEMISKPHGMMLVTGPTGSGKTTTLYG
                     ALQKINDPGKKIITIEDPVEYQLGGVNQIQVKPQIGLSFANGLRSIVRQDPDVIMVGE
                     IRDAETAEIAVQAALTGHLVFSTLHTNDAAGAISRLLEMGVQDYLLSSSLLGVLAQRL
                     VRRLCASCRKEVPFAGFNGNEVELPLQSVGRPQTVWEAMGCDRCSGTGYLGRVGIFEL
                     LPVASEICKVIVQRADAGMIRSLAVQQGMRLLREDGWEKVRQGVTTVAEVLRVTREET
                     "
     gene            complement(2185..2556)
                     /locus_tag="VFN58_03750"
     CDS             complement(2185..2556)
                     /locus_tag="VFN58_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8917137.1"
                     /translation="MAQQTYQGKIVEKPWGYELIWAHTPRYVGKILHINKGESLSYQY
                     HRVKDETVRLLSGSLELEVELAGERTKLKFKPGDCLHIVPGMKHRMTGLDECDVLEVS
                     TPELDDVVRLEDRYGRAGTKD"
     gene            complement(2602..3831)
                     /locus_tag="VFN58_03755"
     CDS             complement(2602..3831)
                     /locus_tag="VFN58_03755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET8917138.1"
                     /translation="MDKLIAWWGKGIFVALCFSIVLLGTGPLDSSLPGVGVRAWSVSR
                     TTFFMWLIWKLLIGVRYGGSQLGFRKHAVPIPLLLFFTFVTISLFPDYFQRAGDYRYF
                     FLGAMHYLMISDLCSNQKRWRLIFYLLALSPGLLAVRGILYDPLVLELDQMRRFEYPL
                     PHPNIAGYLFAMTLPLCLAIAVGERGKLRAFSLLSCGVQLLALILTYSRGSWLGWSAS
                     IFSFGVMLKRWREIWVILTVVGLLLAFATPLQERLFTLFRPQTDFSINERMLAIDAGL
                     KLGLNHPVLGVGYGRGRLREGLAELERGGVLDIGHIAHTHNMYVELFAQTGLLGLGTF
                     LWLLFQAVSQTLSSASRFENADRVFALGIAAAWIAFAVTGLGDVPFYHHEIRIMFFTL
                     LALTHLYSYPSAPSAGN"
     gene            complement(3920..5332)
                     /locus_tag="VFN58_03760"
     CDS             complement(3920..5332)
                     /locus_tag="VFN58_03760"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET8917139.1"
                     /translation="MLKQRSEFFQSLMMLNDLFFLSVAWWLAYLIRFETDLFPEPEPH
                     LLPHYMIAWLVILIVWTAVFHWLDFYRPRRLSSQKREIVEVLKGCILAMLVFLGILFL
                     LRGVVLSRIVVVFFGVSSFVFLNLSHVVFREGLRFLRRRGYNLRHVLVIGTPRQVDQL
                     LQKLKAYRQFGLSIAGVYLTQHAPAEIAPVPGGGLRDTAELNRLVKSGTIDQIFVTLP
                     LEQAGKLVEIQQWLDDEPITLHFIPDVGDLAKLRGRMEEFDGLPIISLQSSPLHGWNS
                     CLKRIMDLSIGGLALLLFSPLMLLIAIVIKLTSSGPIFYRQERMGLDGKRFKILKFRT
                     MIKNAEHTTGPVWATANDPRVTPLGRILRATSLDELPQFINVLRGEMSLVGPRPERPV
                     LINYFRKSIPGYMLRHKVKAGMTGWAQVNGWRGNTSLESRIQHDLDYIGNWSIWRDIK
                     ILALTVFGGFRNKSPGGLRS"
     gene            complement(5326..>5603)
                     /locus_tag="VFN58_03765"
     CDS             complement(5326..>5603)
                     /locus_tag="VFN58_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917140.1"
                     /translation="IAYEKGGVLETVIPVTANTESGGSPTGIFFSEQTPEALSRAVEL
                     YQGMQSRFDPKRIRDHAAQFSTARFRSQISEYVQARLGERWGVTNPC"
BASE COUNT         1432 a         1568 c         1400 g         1203 t
ORIGIN      
        1 gatgttcccc catggatgcc gccagcgatt taccagcgcg caccgcctcc agaagagtcc
       61 gcatcacctt gataaaatcc tcgccctcga tcaaattggc gagcagcgaa aggctgcggt
      121 ccagcggcag cccggccgcc aggagcgtac tcaactcctg ggtgaattga aggagctgtt
      181 gttgattgac ctttcgcttc ggaaacaacg gcaagcgcgt ctgcgagcct gcaattaatc
      241 ggtccccagg catcgcgatc cgtatcggaa tgcagcccat ctcatggagg cgagtcacga
      301 caccttgttc ggcatcggct tccattacac cttcgacgac ttttccagct tggtcggcgg
      361 cacgatattg aaaaaaggcc attgggattg caacgcaaga tagtcgacgc aagaaacagt
      421 tagattctgg gagctatagg ccttactaag ttcaagccag acctaagttt cctctctggt
      481 aactcgcagc acttcggcaa cggttgtaac gccctggcgc accttctccc agccatcctc
      541 cctcaacaaa cgcatgccct gctggacagc caggctacgg atcattcccg catccgcccg
      601 ctggacgatg accttgcaga tctcggaagc cacgggcaaa agctcaaaga tacctacgcg
      661 gccgagatat ccggtgccgc tgcaccgatc gcaacccatt gcctcccaca ccgtctgcgg
      721 tctccccacg ctctggagcg gcaactcgac ctcgttcccg ttaaaacccg cgaacggaac
      781 ctcttttcga caactggcac agagccggcg cacgagtctc tgcgctaaga cacccaggag
      841 cgaagaggag agcaaataat cctgaactcc catctccaac agccgagaaa tcgcaccggc
      901 agcatcattc gtgtgcagtg tggagaaaac caagtgaccc gtcagtgcgg cttgcaccgc
      961 aatctcggca gtttccgcgt cgcggatttc gccgaccata attacatcgg gatcctggcg
     1021 cacgatagag cgcaggccgt tggcgaaaga aaggccgatt tgcggtttaa cctggatctg
     1081 atttacaccg ccaagttgat attccaccgg atcttcgatg gtaatgatct tcttgccggg
     1141 atcgttgatc ttctgcaggg ctccgtagag cgtcgtcgtt ttaccactgc cggtcggccc
     1201 ggtaacgagc atcatcccat gaggcttgga gatcatttca ttaaactgtt ggaggattgt
     1261 ggtaggaaaa cccagagtat ccatctgagt aaagatttgc gcgcgctcca acagacgaat
     1321 gaccacgctt tcaccgtaca gggtgggaac agtggcaata cggagatcga catcttttcc
     1381 cgcaagtcgg atcttgatgc gtccgtcctg gggcagccgg cgctctgcga tattgagttg
     1441 cgccagaatc tttagacgcg aaatcacggc tgccttcagc tggcgaggcg gaggctcgac
     1501 ttcgtggaga atgccatcga tgcggtaccg gaccttcaac tgattctcaa agggctcgat
     1561 atggacatcc gaggcgcggc tctcgagcgc ccgcacgagc atctgattta ccagacggat
     1621 aaccggaacc tcggacgcca tatcacgcaa atgggcgaca tcttcctcat ccaccgcacc
     1681 ttcgatctca cgggcagccg aacctgtgtt ggcttcggtt gaataggaat tgccgaacag
     1741 agcctcgatt ctcgccatga tatctttttc cttggccagc acaggtttaa catgcaatcc
     1801 ggccgcgagt tccagggcat ggaggcgaga gagatccagc gggtcggtaa tggcaacgac
     1861 caagtcccgg ccttcgattt ttatcggtac catgcgggcc tgcttgaaaa aatcccccag
     1921 ggtaggggag aattccacgg ggagcggggt tacgggaaaa tccttaagcg caatcatcgg
     1981 aatctcgaaa tgatccctca gcacaggcag cagatcatcc tcggaaagga atcccagctc
     2041 gacaatcagc cgggtgagcg gcgcctgctg ttcctgctga agtttagcta cccgctccat
     2101 ctcctcgctc gagaggagcc gccgttcaat caactgctct gtgagactca aatgcgaatc
     2161 catattctac ttaaatggtc taagttaatc tttggtaccc gccctgccgt accggtcttc
     2221 cagccgcacc acgtcatcaa gctcaggagt tgatacttcc aagacatcgc attcatcgag
     2281 gccggtcatc ctgtgtttca ttccaggaac tatatgaagg caatcacctg gcttgaactt
     2341 gagcttggtt ctctcacctg ccagctccac ctccaactcc aggcttccgc ttagcaggcg
     2401 aaccgtttcg tctttgaccc tatggtattg ataactcagg gattcccctt tattgatgtg
     2461 gagaatcttt cccacatagc gcggagtatg agcccaaatg agctcgtagc cccatggctt
     2521 ttccactatc tttccctgat aagtctgctg agccattttt taagctggct tcatgatatc
     2581 aaattttctc agaggtggaa actaattacc ggcgcttggg gcggaaggat aactatacag
     2641 atgggtcaga gccaagaggg taaaaaacat gatgcgaatt tcatgatgat aaaaagggac
     2701 atcgccgaga ccggttacgg caaacgcgat ccaagccgcc gcaatcccta acgcaaatac
     2761 tcggtcggcg ttctcgaaac gacttgcgct gctaagagtc tgagatacag cctgaaaaag
     2821 caaccaaagg aaggtcccga gccccagcaa tccggtttga gcgaacagtt cgacgtacat
     2881 gttgtgggtg tgggctatgt ggccgatatc aagcaccccg ccgcgttcaa gttctgccaa
     2941 accctctctg agccgtccac gcccatatcc cacgcctaag acgggatggt tcaatcccag
     3001 tttcaggccg gcatctatcg ccagcattct ttcgttgatc gaaaagtcag tttggggtct
     3061 gaacagcgtg aataagcgtt cctgcaacgg tgtcgcaaaa gctaggagaa gtccgacaac
     3121 cgtcaaaata acccaaattt ctctccatcg cttcagcata acaccaaagg agaagatcga
     3181 ggccgaccat cccaaccagg agccccttga atatgtaagg ataagcgcca acaattgcac
     3241 gccacatgaa agaagtgaaa acgcacgtag ctttcctctt tctccaactg caatcgccaa
     3301 acaaagcggc agggtcatgg cgaaaaggta accggcgata ttcggatgtg gtaatggata
     3361 ctcaaatcgc cgcatctgat ccagttcaag gaccagcggg tcgtacaaaa taccgcgtac
     3421 cgcgagaaga ccgggcgaca gagctaagag ataaaatatg agccgccatc ttttttgatt
     3481 ggagcagaga tccgagatca tcagatagtg cattgctcca agaaaaaaat aacgatagtc
     3541 tccagcccgc tgaaaataat ccggaaataa ggatatcgtg acaaatgtga aaaacagcag
     3601 caggggaatc gggacggcat gcttgcgaaa accgagctgg gagccgccgt agcggacccc
     3661 aatcaacagt ttccagatca gccacatgaa aaacgtcgtc cgggaaacgc tccacgcgcg
     3721 gacccctacg ccgggcaaag acgaatctaa cggtcctgtc cccagcagga caatggagaa
     3781 acagagcgca acaaatatac ccttacccca ccaggcgatg agtttatcca tgtgttgtcg
     3841 ggatttggag gggcactcca tggagtgcag cggggacacg gtcaagggca caggagaatg
     3901 aacaacaaaa tagataaaat caacttcgta ggcctcccgg gctcttgtta cgaaaaccgc
     3961 caaaaacggt aagagcaaga atctttatgt cacgccagat tgaccaattc ccgatataat
     4021 ccaaatcgtg ctggatccgg gactccaatg aagtatttcc acgccatccg ttaacctgcg
     4081 cccagccggt cattcccgcc ttcactttgt gccggagcat atatcctggg atcgatttcc
     4141 gaaagtagtt aatcaatacc ggtctttctg gccgtggacc caccaagctc atttcacccc
     4201 tgagcacgtt gataaactgg ggtaattcgt ccagactagt tgcccgcaat attcgtccca
     4261 gcggagtcac ccggggatcg ttggctgtgg cccaaacggg tccggtggta tgctcggcat
     4321 ttttgatcat cgtcctgaac ttgagaatct tgaacctttt tccgtccaag cccattcgct
     4381 cttgtcgata aaaaatcggt ccggaagaag tcagcttgat cacaatcgcg atcagcaaca
     4441 ttaacggtga aaaaagcagc agcgccagtc cgccgatcga cagatccatg atgcgtttga
     4501 ggcaagagtt ccatccgtgc aacggtgagc tttgtagact gatgatgggt agtccatcaa
     4561 attcttccat tcgtccgcga agcttggcga ggtctcccac gtccggaata aaatggagcg
     4621 taataggctc atcatccagc cattgctgga tttcgaccaa cttgcctgct tgctctaagg
     4681 gtaaagtgac aaagatttga tcgatggttc cagatttcac caatcgatta agttcggcgg
     4741 tatctctgag gcctcctccc ggaaccggcg cgatctccgc cggcgcatgc tgggtgaggt
     4801 ataccccagc gatgctaaga ccgaattggc ggtaggcctt taacttttgc agcagctggt
     4861 caacctgccg gggagttccg ataaccagca catgccgcag attatagccc cgcctgcgca
     4921 aaaatcttaa cccttctcga aaaacgacgt gactgagatt aagaaaaaca aagctggaga
     4981 ccccaaagaa aaccacaacg atccgggaaa gaacgacgcc gcgaagcaag aacaggattc
     5041 ccaaaaaaac cagcatcgcg agaatgcacc ccttgagcac ctcgacgatt tcgcgctttt
     5101 gactcgacaa tcgccggggg cgataaaaat ccagccagtg aaaaaccgcc gtccagacga
     5161 tcaagataac gagccaggcg atcatatagt gtggcaaaag atgcggctcg ggctccggga
     5221 acaaatctgt ttcaaagcgg atcagatagg cgagccacca tgcgacgctc aagaagaaaa
     5281 ggtcattgag catcatcaaa ctttgaaaga actcgctgcg ttgctttagc acgggttggt
     5341 cactccccac cgctcgccca atcgggcctg gacgtattcg ctaatttgac ttctgaaacg
     5401 tgctgtcgaa aattgcgcag cgtggtcacg tatcctcttt gggtcaaatc ggctttgcat
     5461 cccctgatat agctccactg ctctacttaa tgcttccggt gtctgctccg agaaaaaaat
     5521 cccggtgggc gacccgccgc tttcagtatt tgccgtcact ggaatcaccg tctccagcac
     5581 tccgcctttc tcgtatgcga tga
//
LOCUS       DASSWZ010000153         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1349369, whole genome shotgun sequence.
ACCESSION   DASSWZ010000153 DASSWZ010000000
VERSION     DASSWZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349369"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /gene="thrS"
                     /locus_tag="VFN58_03770"
     CDS             <1..1777
                     /gene="thrS"
                     /locus_tag="VFN58_03770"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET8917141.1"
                     /translation="DCSLTWVSIDSPDGLDVLRHSTAHLMAQAVQSLFPGTQVTIGPT
                     IEDGFYYDFKRDRAFTPEEIEKIEARMEEIAKSDLKVSREEMPKSQAIELFRGMGEDY
                     KVAIIEDLPDENVSLYRQGDWVDLCRGPHVPSTGAIKAFKLTGVAGAYWRGNEKNEML
                     QRIYGTAWPTKQALKEHLRLLEEAKKRDHRKLGQELGLFMISDVVGPGLPIWLPKGAM
                     VRSILERYIVDIERSMGYQHVNTPQLARVDLYKRSGHWDHFKDNMYPPMEFENKEELV
                     LRPMNCPHHITIYKHGLHSYRDLPVRLAELGTMYRYERSGVLSGLSRVRAMTLNDAHI
                     FCLPEQIQAETVGVLQLIQRVYRDFGFKDYWYRLSLRNPMDKANFVDNDAMWDRAEGY
                     LRQALNEVGVSFKEAVGEAAYYGPKIDVQLNDVLGHSETLSTVQLDFYLPERFELEYV
                     DRDGQYKRPVMIHRGVISTMERMMAFLIEHYAGNFPLWLAPVQVKVVTVTDQQLDYAR
                     RVSAELTAAGWRVELDERNEKLGYKIREAQLAKIPYAVVIGDKEVEAQTVAPRRRGGE
                     NVAPLSVGDFIQRLKFEVAQERGEA"
     gene            1814..>2295
                     /gene="infC"
                     /locus_tag="VFN58_03775"
     CDS             1814..>2295
                     /gene="infC"
                     /locus_tag="VFN58_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET8917142.1"
                     /translation="MAREARINHQIRAREVRVIGAQGEQLGVMPLYEALKQVDALELD
                     LVEVAPDAQPPVCRMMDYGKFRYEQKKKSHGPKKHTVVDIKEVKMGSRTDPHDVVHKV
                     RNIRQFIEKGQRVKVSVFFRGREITHPELGKQMLSGVVDQVRDIAKVDIEPRLEGRNM
                     A"
BASE COUNT          570 a          559 c          672 g          494 t
ORIGIN      
        1 cgattgctcc ttgacgtggg tgtcaatcga tagccctgac ggcctcgatg tgctgcgcca
       61 ttcgacggcc catctgatgg cgcaggccgt acaaagcctc ttcccaggaa ctcaggtcac
      121 catagggccc accatcgagg atggttttta ctatgatttc aaacgggacc gagccttcac
      181 tccggaagag atcgagaaga tcgaggcgcg aatggaggaa atcgccaagt ccgatctcaa
      241 ggtaagtcgc gaagagatgc cgaaaagtca ggccatcgaa ttattccgcg gcatgggtga
      301 agactacaag gtcgctatca tcgaggattt gcccgacgag aatgtgtcgc tgtaccggca
      361 aggggactgg gtggatctct gccgtggtcc gcatgtgccg tccactggag ccatcaaagc
      421 cttcaagctc accggagtcg ccggcgccta ttggcgcggc aatgagaaaa acgagatgct
      481 tcagcgcatc tatggtaccg cttggcctac caagcaggcg ctcaaggagc atttgcgact
      541 tctggaggaa gcgaagaagc gcgaccatcg caagctgggg caggaactgg gtctcttcat
      601 gatttcagac gtcgtcggtc ccggacttcc catctggctc ccaaagggcg cgatggtccg
      661 ctcgatcctg gagagatata tcgttgatat cgagcgaagc atgggctatc agcatgtgaa
      721 tacgcctcag ctcgcacggg tggatcttta taaacgatcg ggccattggg atcatttcaa
      781 agataatatg tatcctccga tggagttcga gaacaaagaa gagttagttc tccgaccgat
      841 gaattgcccc catcatatca ccatctacaa acatggactt cacagttacc gcgatttgcc
      901 ggttcggctg gcggagttgg gaaccatgta tcgctatgaa cgctctggcg tgctttctgg
      961 tctttcgcgc gtgagggcga tgaccctgaa cgacgcccat attttttgtc ttcccgagca
     1021 gatccaagcc gaaacagtgg gagttctcca gctaatccaa cgcgtatacc gggattttgg
     1081 cttcaaggac tattggtacc gtctttcgct gcgcaatccc atggacaaag caaatttcgt
     1141 tgacaatgac gccatgtggg atagggcgga aggatatcta cggcaagctc tcaatgaagt
     1201 cggggtgtct ttcaaagaag cagtgggcga ggccgcatac tacggtccga agattgacgt
     1261 gcaattgaat gacgtgctag ggcatagcga gactctgtcc accgtgcaac ttgattttta
     1321 tcttcccgag cgattcgaac tggaatatgt tgacagggac gggcagtaca aacggccggt
     1381 catgatccac cgtggcgtca tcagtaccat ggaacggatg atggcttttt taatcgagca
     1441 ctatgcgggt aattttcccc tctggctggc ccccgtgcaa gtcaaggttg tgacggtgac
     1501 ggatcagcag ttggattatg cgcgaagggt ttcggcagag ttaacggcag cgggttggcg
     1561 ggtggagttg gacgaacgca acgaaaagct cggttacaaa attcgcgagg cccaattggc
     1621 gaagattccc tatgcggtcg tgatcggcga taaagaagtc gaagcgcaaa ccgtggcgcc
     1681 gcgccgcaga ggcggtgaaa atgtggcgcc gctgtccgtc ggcgatttta ttcagaggct
     1741 gaaatttgag gttgcacagg aaaggggaga agcatagaaa tcaagagagt cttacaacaa
     1801 acggaggtgg cttatcgcta gagaagcgag gatcaatcat caaattcgcg cccgtgaggt
     1861 ccgtgtcata ggcgcccagg gagaacagtt gggcgttatg ccgctctacg aagccttaaa
     1921 gcaggtagac gcactggagt tggaccttgt ggaggtggcg ccggatgccc aaccgccggt
     1981 ttgccgcatg atggactatg gtaagtttcg ctatgagcaa aaaaagaagt cgcatggacc
     2041 gaagaagcac accgtcgtcg atatcaaaga agtcaagatg gggtcacgga ccgatcccca
     2101 cgatgtcgtg cataaggtcc gaaacattcg ccagttcatt gagaagggac aacgcgtcaa
     2161 agtatccgtt ttcttccgtg gacgtgagat cacccacccc gagttgggta aacaaatgct
     2221 gagcggagtc gtcgaccaag tccgagacat tgctaaagtt gacattgaac caagattaga
     2281 ggggcggaac atggc
//
LOCUS       DASSWZ010000154         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_132199, whole genome shotgun sequence.
ACCESSION   DASSWZ010000154 DASSWZ010000000
VERSION     DASSWZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132199"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFN58_03780"
     CDS             <1..229
                     /locus_tag="VFN58_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET8917143.1"
                     /translation="WTVQVASLNQTRDAEGIAGKLKGKGYDAYVVAAEVKSKLWHRVR
                     VGQGVDLSEAFELRARLKAKENFDQAFIALR"
     gene            471..668
                     /locus_tag="VFN58_03785"
     CDS             471..668
                     /locus_tag="VFN58_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917144.1"
                     /translation="MGAVPVRAQIELIHSTFEDHLNGLSKWCLGGYERLATLILAPKG
                     AMVTRFPEAKTVVGHLDLYYK"
     gene            668..1603
                     /locus_tag="VFN58_03790"
     CDS             668..1603
                     /locus_tag="VFN58_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007813469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET8917145.1"
                     /translation="MGEAIVNPNELTIEQVQDILRAERYICDRSLATVVYLSLIMGKP
                     LLLEGEAGVGKTEIAKVLSKVLGAKLIRLQCYEGLDANTALYEWNYPKQMLRIKLEEV
                     EHGDKETVETEIFTQDYLIKRPLLEAIQTDGAGPPVLLIDEIDRADMEFEAFLLEVLS
                     DFQITIPEIGTIAARHRPYVFLTSNRTREIHDALKRRCLYHWIEYPSFEKEYEIITTK
                     FPEVEANMAKQICAFMQKVRQMNFYKRPGVAETLDWASALIALNRRELDDKTVVETMG
                     CVFKYREDLHHLKEQVEGKQLNLEALLRMSPELVS"
     gene            1609..2820
                     /locus_tag="VFN58_03795"
     CDS             1609..2820
                     /locus_tag="VFN58_03795"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8917146.1"
                     /translation="MEVTPEAVASAVSNVSSVRGQGTLPNLLAFGRALKELGIKVSLS
                     QVMDVSRAVEFVDLADKGDFRALLRSNLISQKEDFPVFDMLFECFWREQSYERVPMET
                     MEIQGTPTESGAEEGGDEEGLEEAVAETVATENVPLENLDEFSVPTYSPQELLNRKDF
                     SEMGVEESRAIARAILLIATKIATQISRRKKLGRKGNVVDPRWTMRKNMKYGGEIIDL
                     VNRKRRIKKTRVVLLCDVSGSMDCYSRFLIQFMYGLQNELWGVETFVFSTSLSRITHL
                     IRTKDIANALEKISGSILGWSGGTNIGRSLHTFNRNFAPSVVTHRTVVVIISDGWDRG
                     DVSLLEREMQDLKRRCKKIIWLNPLLASDNYEPLCKGMQAALPYLDLFLSVHNVNSLI
                     ALGRTLQKMVA"
     gene            2852..3247
                     /locus_tag="VFN58_03800"
     CDS             2852..3247
                     /locus_tag="VFN58_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET8917147.1"
                     /translation="MPGQGTCFDDANPEAKKKDSVYHQVKEFLDKGETMAVATIVSTK
                     GSTPREVGAKMVVTAWGEILGTIGGGCGEADVKREAIEVIRTRKPRMVRIDLLDDISS
                     DSPAVCGGIMNVFIDPWWKERDQEAVTSK"
     gene            3244..>3918
                     /locus_tag="VFN58_03805"
     CDS             3244..>3918
                     /locus_tag="VFN58_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET8917148.1"
                     /translation="MSSLSEELVQVAEQGAAAVLATVIEVEGNERVDAGAKCLIRDGQ
                     VRAETIGDAKVVEAILRESAVHLQEEKSKLVSLNIPQDGGKLEVFFEVMPAPPKLVVV
                     GAGHIAVPLVKIAKVLDFYVIVTDDRLAYANRERFPDADEVLIGDMAQMLKEMSITPS
                     TYIVLITRGHKYDEPCLREIIHSQAKYIGMIGSRRRIKACFQRFHDEEKIAEEVIDRV
                     YAPIGLD"
BASE COUNT          928 a          950 c         1161 g          879 t
ORIGIN      
        1 ttggactgtt caggttgcct cactaaacca aacccgcgat gcggagggga ttgccggtaa
       61 gctaaagggc aaaggctacg acgcttatgt ggttgccgct gaggtaaagt ctaagctttg
      121 gcatcgcgtc cgcgtgggcc agggagtcga tcttagtgaa gcctttgagc tacgagcgag
      181 actcaaggcc aaagagaatt ttgaccaagc atttatcgcg ctccgatagc taagctgcac
      241 cacgtcggtt tcggagcgcc gttttttccg cgcttttttc tcgcaggtgc attccggacg
      301 atctccggaa tgtacctgct tcctgccgct cagcgtcatt tcggttcgcc taacattgca
      361 gaatgcctgc cgcgcgctga tttgcgactt gctccgctag gattttcata agccgcgcca
      421 ctttgttatg atggaattgg tgatcggcat caaccggtat tgccggcgag gtgggtgcgg
      481 ttcctgtccg ggctcagatc gagctgattc actctacgtt cgaagatcat ttgaacggtc
      541 tttccaaatg gtgtttggga gggtatgaaa ggctggcgac attgatcctc gcgccgaaag
      601 gggctatggt gactcgattt ccagaggcca agactgtggt ggggcatctg gatctctatt
      661 ataaataatg ggtgaggcga ttgtgaatcc aaacgagcta actattgagc aagttcaaga
      721 cattctcagg gctgagcgct acatctgcga tcgctcatta gccaccgtcg tctatctttc
      781 gttgatcatg ggtaaaccct tgcttttgga gggcgaggcg ggagtcggta agacagagat
      841 cgccaaggtg ctttccaagg ttctgggcgc caagttgatt cgcctgcagt gttacgaagg
      901 attggatgcc aacacggcgc tatacgagtg gaactatccc aaacagatgt tgcgcatcaa
      961 attggaagag gtggagcatg gggataagga aaccgttgag accgaaatct tcacccaaga
     1021 ctatttgatc aagcggccgt tgttggaagc gatccaaacc gatggcgcag gccccccggt
     1081 gctgctgata gatgagatcg atcgcgccga catggaattc gaagcgttct tgttggaagt
     1141 gctttcggac tttcagatta ctatccccga aatcggcacc atcgcggcaa ggcatcggcc
     1201 gtatgtcttt ttgacctcga atcgaacccg cgagattcat gacgcgctga agcgccgctg
     1261 tctttaccac tggattgaat acccgagctt cgaaaaggaa tacgagatca ttaccaccaa
     1321 gttccctgag gtggaggcga acatggcaaa acagatctgc gctttcatgc agaaggttcg
     1381 ccaaatgaat ttctacaagc ggcccggcgt ggcggaaacg ttggattggg cctcggcttt
     1441 gatcgctctc aaccgcaggg aattggacga caagaccgtg gtggaaacca tgggctgcgt
     1501 tttcaaatat cgcgaggacc tgcaccatct aaaggaacag gtcgaaggaa agcagcttaa
     1561 tctggaggcc ttgctcagga tgtcgcctga gttggtaagt taataaagat ggaagttacc
     1621 ccggaagccg tcgcctccgc cgtcagcaac gtttcctcgg tgcgtggtca aggaaccctg
     1681 cctaatcttt tggccttcgg gcgggcctta aaagagcttg gtattaaggt cagcttgagc
     1741 caagtgatgg atgtttcgcg cgcggttgag tttgtcgatc tcgcggacaa gggggacttt
     1801 cgcgccttgc tgcgctccaa tttgatttcc caaaaagaag actttccggt cttcgacatg
     1861 ctattcgaat gtttttggcg ggagcagagt tacgaacggg tgcccatgga gacaatggag
     1921 attcagggaa cgccgaccga atccggagcg gaagagggag gcgatgaaga aggattagaa
     1981 gaagccgttg cggagaccgt cgccacggaa aacgtgccgc tggagaacct cgacgagttt
     2041 tccgtgccta cctacagtcc gcaagagctg cttaatcgta aagacttcag cgaaatgggc
     2101 gttgaagaaa gtcgcgccat cgcgcgagcc atcttgctca ttgcgaccaa gatcgcgacg
     2161 cagatcagcc ggcgcaagaa gctcggacga aagggcaacg tcgtggatcc gcgctggacc
     2221 atgcgcaaga acatgaagta cggcggcgag atcatcgacc tggtcaatcg caaacgacgc
     2281 atcaagaaga ctcgggtcgt gctgctttgc gacgtgagcg gttcgatgga ttgctacagc
     2341 cgattcttga ttcagttcat gtatgggctg cagaacgagc tctggggggt ggagaccttt
     2401 gtcttcagca cttccctcag ccgcattacc catctgattc gcaccaaaga catcgctaat
     2461 gccctggaaa aaatttccgg cagcattctc ggttggtcag gtggaaccaa catcggccgc
     2521 tccctgcata cgttcaaccg caactttgcc cccagcgtgg tcacccatcg cacggtggtc
     2581 gtcatcatca gcgacggctg ggaccgtggc gatgtgagtt tgctcgagcg ggagatgcaa
     2641 gacctgaagc gcagatgcaa gaagatcatc tggctcaatc ctctgctggc gagtgacaat
     2701 tacgaacctc tttgtaaggg gatgcaggcg gcgctcccct atctggacct atttttatcg
     2761 gtgcataacg tcaatagcct gattgcgtta ggccgtacac tgcagaagat ggtggcataa
     2821 cgaatctgga acaataccag gaggtgaaac catgccaggt caaggaacat gctttgatga
     2881 tgcgaatccg gaagccaaga aaaaggactc ggtctatcac caggtgaaag agtttctcga
     2941 taaaggagaa acgatggcgg tggcgaccat tgtgtctacg aaaggctcga caccacgaga
     3001 agtcggcgcc aagatggtgg tgaccgcatg gggagaaatt ctcggcacca ttggcggcgg
     3061 ttgtggcgaa gcggacgtga agcgggaggc cattgaagtg attcgcacgc gcaagcccag
     3121 aatggttcgg atcgatctct tggatgatat ttcgtccgat agtccggcgg tgtgcggtgg
     3181 gatcatgaac gtgttcatcg atccgtggtg gaaagagcgc gaccaggagg cagttactag
     3241 caaatgagtt cgctgtctga agaactcgtc caggtcgccg agcagggcgc ggcagcggtg
     3301 ctggctacgg tgatcgaggt cgaaggcaat gagcgggtgg acgccggtgc caaatgcttg
     3361 attcgtgacg gccaggtacg agctgagacg atcggcgacg ccaaggtcgt tgaggcaatt
     3421 ctccgagaaa gcgcagtcca tcttcaagaa gaaaagtcaa agctggtctc actgaatatc
     3481 ccgcaggacg gaggcaagct ggaagtattt ttcgaggtca tgccggctcc accgaagctg
     3541 gttgtggttg gtgctggcca tattgccgtg cctctagtga agatagcgaa ggttttggat
     3601 ttctatgtga tcgtaaccga tgatcgacta gcgtatgcca atcgcgagcg ttttccggat
     3661 gcggacgaag tcctgatcgg tgatatggcg cagatgctta aggagatgtc gattacgccg
     3721 tcgacctata tcgtcctgat cacccgaggg cacaaatacg atgagccttg cttgcgtgag
     3781 atcattcaca gccaggcgaa atatatcggc atgatcggca gccggcggcg gatcaaagct
     3841 tgtttccaac gatttcacga cgaagagaag attgccgaag aggtcatcga tcgggtttac
     3901 gcacccatcg ggctcgat
//
LOCUS       DASSWZ010000155        13545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_316481, whole genome shotgun sequence.
ACCESSION   DASSWZ010000155 DASSWZ010000000
VERSION     DASSWZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13545
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316481"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..2474)
                     /locus_tag="VFN58_03810"
     CDS             complement(393..2474)
                     /locus_tag="VFN58_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8917149.1"
                     /translation="MATVAEAAVPAGASLVPPVLVFLFTIVAFLPTLQNGFVNWDDEA
                     FLVSNPNYRGLGWKQIQWMFTTCYLSSCMPLTWVTYGLDYVLWGMNPLGYHLTSLLIH
                     AANAVLFYFVSLRLLTLALSRSALSWQLSIRVAAGFAALFFALHPLQVEVVAWTIGRE
                     MAVAGFFFFLTLLCYLKAAEHESNRESYWRWMGATWLFYTLSLLGKEVAMTLPFALIA
                     LDVYPLGRLGGGRETWFGPQVRRVWWEKIPLLLVTLAAGVRAVLGKEGTGAVYPLAGY
                     GLLPRVAQVLYSLAFYPWKTLAPLDLSPLYPLHGFTGLWELRWLLSGAFVVLLTAGFF
                     IARRRWPAGLTAWVFYVVLLIPVSGIVTFGPQLVADRFSYLPSLGWAILLGAAIFHCW
                     KLWMSGRMSVRNFVSTQSLAVLLLVGLGVLTWHQTRIWHNSERLWRHALALDQKSSFA
                     HNNLGLALANRGAFAEAINEFRKAVEIDPAFVEAHTNLGIFLAQGGSSEEAVSHLRQA
                     LTIDPTFASAQNTLGNILADRGESDKAVEHFRKALQSNPKSAMTYYNLGRVLAKRGDL
                     EGATECYRKALELNPLDPDVHNNLGLVFQSQGKSQEAAAQFNNALRVEPRYARAYFNL
                     GKLFAGQGNFSSAVENFRQALQIQPGLPEIHEQLARALALQGKKEEALQHYREAVRLV
                     KSRREEFEPGK"
     gene            2768..5668
                     /locus_tag="VFN58_03815"
     CDS             2768..5668
                     /locus_tag="VFN58_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917150.1"
                     /translation="MKRFALRAALFSIAILILAYVSIFFFVRSDGFQQWLKAEAIQRS
                     GYQINLSDLRLMFPFRLAASGLTVSNESETLLQGDRIVVTFSLVDFFSKSIFRIKLEK
                     PILRLDLPKLFDSSAKNSIDIAIRHLNVEDGTVILRTVEGQSLEFRAVNLNAQHVNMG
                     QASGITLQTDLPWLTASAEISIHSEKEEQEAEIKVRQAPTQDLTRLLSRKTPPRDALN
                     IKFKVRKAHSQALAVSASGQVDGLAIGAEKINGRFESRAEIDPDFKNTTVLANIEASD
                     LPSQIGSVQLPAISGGAVGNLEGTYSFAERTMTFKALHFTSASGTADGRGVVFFGPEP
                     TFANIHMNLSKVPVTAIRPFLPEPIRAWTLRGSAEADLQIQGPWRSIAIKGVARANGA
                     ELKSETFSLQQLNLTAPFEWVNSSLRAGDVRIQGKTLALKSSAVQFTAEELQLDGGLE
                     SKPGQPLRTNSKLRLLRGRYATADGSKMGENFNLAGHLDMTNGAKNLFSASGSLNIEE
                     GEILWGKFFGDFKSQKPSIDFDGDYSAGEDELRLRRLDLSVANVGQVGLSGTLQQFSQ
                     RPIASIQVNGTNIQPSSVFDFFIRETLSRSHPILDQLTLGGRVDLAARVSGTLDDLSV
                     DGNLQVRRGSLRVKSDKWLVGPINLALPFRIHLPAATPEGSSATIPAGTLAIESFRFG
                     AEPIPGIKTPVSLWNNTLKFGQPIRLSIYGGTIEIRNLAWADLIEDPLSFSLAIEARD
                     LQLQRLTESLGWYRFGGTLSGSIPKVEMAGNVLRSEGQIEIGVFGGQVHVSTTEIENP
                     FSSLPAVKLNARFQDIHLEQASETFAFGRISGILEGTISDLIIANGQPSQMQADVHTV
                     ARSGSSQWISVEALNKITVLSSGEDGSLIYGGIASFFDEFRYSKMGFKASLRNDKLTL
                     RGIESSDGKEFLVVGSLLPPTVNVISHTREIGFSDLMKRLEQIQKSDKPQIK"
     gene            5682..6260
                     /locus_tag="VFN58_03820"
     CDS             5682..6260
                     /locus_tag="VFN58_03820"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HET8917151.1"
                     /translation="MLKRVALFIAFLANACVTVNIYFPAAAVERAADEIVKETWGSRT
                     ESAPREQPRSLLPAAWKIASVSFIDEAFAQEADINISNPAIRALKDSIKNRSGSIKPF
                     MDRGNVGLTRDGLLTVRTTDGLNLKERAEVQQLVDAENRDRESLYLEIAKANNFPKES
                     VPKIKSIFAKSWAEQAQSGWWIQDAQGNWQKK"
     gene            complement(6283..7098)
                     /gene="proC"
                     /locus_tag="VFN58_03825"
     CDS             complement(6283..7098)
                     /gene="proC"
                     /locus_tag="VFN58_03825"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET8917152.1"
                     /translation="MLKEKIGIIGAGKIGSAIVRGMINVGLVARDQVIASDVSDALRQ
                     SIANELGIEVTPDNGKVCDFADSVILAVKPQIVDAVLKEVAKQLGKAKLLVSVAAGVP
                     LSRLETHLVQGARVVRVMPNIPCVVGAGASGYAGGAHATAKDMETVGSILNSFGIALP
                     VEEKYLDAVTGLSGSGPAYVFLFIESLADGGVQVGLSRDVALKLALQTVYGSARMALE
                     SGKHLGELRDEVTSPGGTTIAGLFALEQKGFRGTVMEAVVNATRRSQELGKGQ"
     gene            complement(7253..10687)
                     /locus_tag="VFN58_03830"
     CDS             complement(7253..10687)
                     /locus_tag="VFN58_03830"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET8917153.1"
                     /translation="MTETFHRSKQDWLLGVGLFCFFLSGAAGLIYQVVWTRMLTQIFG
                     NTTYAIATVLSAFMAGLAIGSYLFGKIADRGKNDFLLYGILEAGVGIYGFAVPWLFRM
                     AQKVYGPIFGLNESYPFLFNMVLFLLSFTLLVFPTLLMGATLPVLSRFFVRSFAQFGR
                     RVGDLYATNTLGAVIGCAAGGFLLIPALGMRATVFVAATVNLVIAALILVVDRIRDKE
                     SLVFTAETVAEEAKAAEGEAAPSWLLWVLLISFGLSGFASLVYENAWTRALTLVIGSS
                     IYSFTTMLVTFLIGLSLGGFIYARFLGNREARLSTFGLIELWVGLAALATIPLFERLP
                     LIFVRLLQGFGDTFTVFLYLQIFLSALVMFVPTVLLGMTFPLVARLFTQSLYRVGSGV
                     GSSYAANTVGAVLGAFAGGFILIPNIGVQNTIVFAVVMNLLIGCLLVWADPRMSMVPR
                     FALGLVVLVLAVLIPFRVPRWDHHVLTSGVTIYHDRYEGLPTNSLRLEEMKRDDVLFY
                     REGLTTTVSVHRIPGSEYIYFKSNGKIDGSYGDALSQLMTSYIPMLLHPKAERALTIG
                     LGSGMSAKALATFDTLKEIEVIEIEPAMIEASKFFDRAFVKMDKLPPGVSIPVDDGKS
                     RVWYDPSKQLLFYKGVMKDEERSRLMKLSEDRDYRGAIDTLHRRARNSRHSSVLEDKR
                     VRVIPTDGRNYILATPKYYDVITAEPSNPWIAGIANLYTREFYQVIKSKIKDDGIFAQ
                     WFHNYSMSPDDFRMVFRTFVEAFPYVSLWSMKESDFLLIGSKQEHPFDYAAVKKIYDS
                     NWMLKSDLDYLGLSDVYAVQGFYRMGKENLLNFSKGADINTDDGAQLEFSAPKSLRRA
                     TTELNRKIMMPYIVDSPPWLKNKPLPVPEAMHHYYMAQSYVASVSNNRALNELEQAIR
                     LDPTNPKFYVLQTKILLDQDKSVEASKAALAAMERSRDTTSDILALSDEFYLPEAKIV
                     YAKAIELGSREVLPYLGLGNIALHSGDVREAERWFEPARQIQADHPAVLLAYGRLALV
                     KGNREKDKEAATRLFQEAKGLLEKSKGRGEDSATLYSELGEVYVKLNMWDKAVESYKE
                     ALRMRRRRNDWRRSLGEAYAKLGKVREAEQKYREVLALSPDDDQALHGLQALGKRY"
     gene            11047..12621
                     /locus_tag="VFN58_03835"
     CDS             11047..12621
                     /locus_tag="VFN58_03835"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8917154.1"
                     /translation="MAKAAKRDGAAPEEIRDLRGAIEWMKKEGDLIITEKEVDPNLEI
                     TGIQKQLDGSCPILFNNVKGKPKNRCITNLFGDMNVINKMFGWKDHADRTRKLAYALT
                     HPIPPVIVDQKDAAVQEEVYEKPAEANEFVVPIRHTDVEPELTVGSGNRLISGEYFGG
                     GTDLGYNRMNFRWGNVGTFQISPGSHMWQVVSKHYKDNKLVPITMNFGLPPAGSLLAG
                     AGFDYVILPQGCDEVGIAGAVQGAPMRLVKARTVDAMALADAEVVLEGYVNPRDRRFE
                     TAESEKAGIQGRFHFHPEWAGYMGKAYKAPTFHVTAVTTRRRDSKPIIFTLGVHTLDD
                     HNIDTTVREAAMFELCERMQPGIIMDVNIPYCMTDWGGAIIQVKKRNRIEEGWQRNFM
                     AAILATSQGSRLCIAVSEDCDPYDMDDVMWCLTTRVNPKTDIINPLPGGRGQTFMPAE
                     RMTAGEKEWTASNTSFEGGMGIDATVPFGYESDFMRPVYPVERIDLKKFFHDKDIQNA
                     KSRMQGWVHSLARTGR"
     gene            12690..>13545
                     /locus_tag="VFN58_03840"
     CDS             12690..>13545
                     /locus_tag="VFN58_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET8917155.1"
                     /translation="MASTDRNAGLLSPYRVLDLSNELGFLCGKILGDLGADVIKIEPP
                     GGDPARRLGPFYGDQPDPEKSLFWFGFNNNKRGITLNLESAKGRELFSQLVTKADFVV
                     ETCTPGYLDSLNLGYKMLSMINPRLVLTSITPFGQTGPYSRFKASDIEIMALSGCMSL
                     TGDPDRPPLRVTFPQSYGWTGSYAAMGALIAHHYREQTGEGQQVDVSAQACLLWAFSH
                     AHTFWDLNRHVEKRAGSFMTGRSITGAKMRVFWPCQDGYLNFIIYGGEAGRRTNQALV
                     EWMDSKGMA"
BASE COUNT         3330 a         3847 c         3473 g         2895 t
ORIGIN      
        1 gcggcggtat gccaccgctg tggtaccaag atttatccgg cggagcaact cgaaccgcac
       61 ttggaccgcc atcagttgaa agatctctat ctggaaggtg aactgaaaaa gctgcagttc
      121 gctatggcaa gaatgagata aagcttgatt ctcgccggct acgggtcgga tcaccagtgt
      181 ttcgcgagcg caagctcgaa cacctaggcg ttacggttcg ggtttgcggc taccaacccc
      241 agggtgtccc ctccataggg gacaccccct cccccaaaaa ctcaaatatc gctggattca
      301 caccccacgg cactctatct gggacgtttg gggtcacccg ggttgttgtc caaacgtcct
      361 ccctagacca taaagcaggc aagcaagacc aactacttgc caggttcaaa ctcttcccgg
      421 cgcgatttca caagtcttac ggcttcccgg tagtgttgaa gggcctcctc tttttttcct
      481 tgcagcgcaa gcgcacgcgc caattgctca tggatctcgg gtaatccggg ctgaatctgc
      541 agcgcctgac gaaagttttc gacagcgcta ctgaaatttc cctgcccggc aaaaagcttt
      601 ccaaggttaa aataggctct cgcatatctc ggttcaacgc gcaaggcatt gttaaactgc
      661 gcagcggctt cttgcgattt accctggctt tgaaaaacta gacctaagtt gttgtgcacg
      721 tcgggatcta gcgggttcag ctccaaggcc tttcgatagc attcggttgc cccttccaaa
      781 tctcctcgct tggcaagaac gcgacctaag ttatagtagg tcatcgcaga ttttggattt
      841 gattgcagcg ccttgcgaaa atgttcaacg gccttatccg actcgcccct gtcagccaag
      901 atatttccca aggtgttctg ggcactcgca aatgtcggat cgatcgttag agcctggcgc
      961 aggtgggaga ctgcctcctc ggacgaaccg ccctgggcga gaaaaattcc cagatttgtg
     1021 tgagcctcca cgaatgcggg atcaatttcg acggctttgc ggaattcgtt gatcgcttct
     1081 gcaaaagcgc cgcggttcgc aagcgcaaga cctaaattgt tatgagcaaa actcgacttt
     1141 tgatcgagcg ccagagcatg tctccacagt ctctcagaat tgtgccagat ccgggtctgg
     1201 tgccaggtca aaacccctag acccaccaag agcagcacgg ccaaagattg cgttgaaaca
     1261 aaatttcgca cactcatgcg gccgctcatc caaagtttcc agcaatgaaa tatggctgct
     1321 ccaagcaata tcgcccagcc gagggacggc agataactga agcgatccgc tacgagctga
     1381 ggaccgaagg tgacaatgcc actgaccggg atcagcaata ccacgtaaaa gacccaagct
     1441 gtcagaccgg cgggccagcg gcgcctggca ataaagaaac ccgcagtgag caacaccaca
     1501 aatgccccgc tcaagagcca tcgcagttcc cacagtccgg tgaaaccatg caaaggatag
     1561 agtggcgaca agtcgagcgg ggcgagggtc ttccatggat agaatgccag gctgtagagc
     1621 acctgcgcga ctctcggcaa cagcccatag cccgcgagag gataaaccgc tcccgttccc
     1681 tccttgccca gtaccgctct cactccggca gccagggtca ccaacagcaa aggtattttt
     1741 tcccaccata cgcgacgaac ttgtggcccg aaccatgttt cccgaccgcc tccgagtcgg
     1801 cccaatggat agacatccaa cgcaataagg gcaaaaggaa gcgtcatggc gacttctttg
     1861 ccgagaagag acagggtgta gaaaagccag gtcgccccca tccatcgcca ataagattcg
     1921 cgattggatt catgctccgc agccttgaga taacaaagca gagtcaaaaa gaaaaaaaag
     1981 ccggcgacgg ccatttcccg gccgatcgtc catgccacca cctccacttg caaaggatgg
     2041 agagcaaaga agagcgcggc aaaaccagct gccactcgga ttgagagttg ccaagagagc
     2101 gctgagcgtg acagagcaag cgttaacaat cgcaaactga caaaatagaa caaaaccgcg
     2161 ttagcggcgt gaataagtag actggttaga tgatagccca gcgggttcat tccccacaaa
     2221 acataatcca acccataagt cacccaggtc aagggcatgc aagaactcaa atagcaggta
     2281 gtgaacatcc actgaatttg tttccaacct agaccccggt aattaggatt gctgaccagg
     2341 aacgcctcgt catcccaatt gacaaagcca ttttgcagcg tagggagaaa ggccacgatc
     2401 gtgaacagga atacgagaac cggcggtact aagcttgccc cagccggcac cgcagcctct
     2461 gcaaccgtgg ccatctgcct gtaggttgcc tggtttttaa ctctcgatct ttttctacct
     2521 gacttcacca gatatgcact cagttggagg ccaagttctg ctaaccccaa ggggctctac
     2581 cgcaccttct tagctactta ctcaacgacc agcaccttcg agcccgttcc ggcaagccta
     2641 ttgcacgctc aaatattatg tcaaaataac agccccatcg gtcaaaggga aaccgccgga
     2701 taggccatgt aattttgaga ttcattgctc gtcaaaccat cggaatgtat cgttaccggg
     2761 gatcactttg aagagatttg cgctcagggc agcactcttt tccatagcca ttctaatcct
     2821 ggcctatgtt tcaattttct tctttgtgcg atcggatggt tttcagcagt ggctcaaagc
     2881 cgaagccatc cagagatccg gttatcaaat caatctgagc gatcttcgac tgatgtttcc
     2941 ctttcggctg gccgcctccg ggctcacggt ttccaacgaa tcagaaacgc tcctccaagg
     3001 agaccgaatt gtcgtcacct tcagtcttgt tgatttcttc tccaagagca tcttccgaat
     3061 caaactcgaa aaacccatct tgcgccttga cctgccaaag ctgttcgact catcagctaa
     3121 aaattcaatc gatatcgcga ttcgccattt gaatgtcgaa gacggaaccg tcatcttgag
     3181 aactgttgag gggcaaagct tggagtttcg cgccgtcaat ctgaacgcgc aacacgtcaa
     3241 tatggggcaa gccagcggca ttacactgca gacggatctg ccttggctca cagcctcggc
     3301 ggaaatctcg attcacagcg agaaggagga gcaggaggcc gagatcaagg tccgtcaagc
     3361 gcccacccag gatctcaccc ggctgttatc aaggaaaacc ccgccccgtg atgctctaaa
     3421 catcaaattc aaggtgcgca aagctcatag ccaggcattg gcggtgtcgg cctcgggaca
     3481 ggttgatgga ttggccatcg gtgctgaaaa aataaatggt cgcttcgagt cgcgcgctga
     3541 gattgatccg gacttcaaaa acacaaccgt cttagcgaac attgaggcga gcgaccttcc
     3601 ctctcagatc ggttccgttc agctgcccgc catttccgga ggcgccgtcg gcaatcttga
     3661 aggcacttat tctttcgcag aaagaactat gaccttcaag gcgcttcatt tcacttccgc
     3721 ctcaggaacg gctgacggca ggggcgtagt gttttttggt cctgagccga cttttgcgaa
     3781 catccacatg aacctaagca aagttcccgt gacagctatc aggccattcc tgcctgaacc
     3841 gataagggcg tggactctcc gcggctccgc ggaagctgac ctgcaaatcc agggaccctg
     3901 gcgctcaatc gcgatcaaag gtgtggctcg agcgaacgga gctgagctaa agagcgaaac
     3961 attttcgctc cagcaactta accttacagc tccgtttgaa tgggtcaatt cttcgttacg
     4021 tgccggcgat gtccggatcc agggaaaaac attggcgcta aagagcagcg cggtgcaatt
     4081 cactgctgaa gagttgcagc ttgacggcgg gctggaatcg aaaccgggtc agccactccg
     4141 aaccaacagc aagctccggc tcctgcgcgg tcgctacgcg actgccgacg gttccaaaat
     4201 gggagaaaac ttcaatctgg ccggtcattt ggatatgacc aatggagcga agaatctttt
     4261 ttcggcgtct ggcagcctaa acatcgaaga gggtgagatc ctctggggaa agtttttcgg
     4321 agatttcaag tcccagaagc cttccattga ctttgatggc gattactctg ccggtgaaga
     4381 cgaacttcga cttcgcaggc tggatctttc agtcgctaac gttggtcaag ttgggctgag
     4441 cgggacttta caacaattct cccaaaggcc aatagccagc attcaagtca atggtaccaa
     4501 tatccagcct tcaagcgttt ttgacttctt catccgtgaa acgctcagcc gcagccaccc
     4561 gatcttggat cagctaaccc tgggcggccg cgtcgatttg gccgcccgag tgtctgggac
     4621 tttggatgat ctttccgtcg acgggaatct tcaggtgcga cgcggcagcc tgcgcgtcaa
     4681 atccgacaaa tggctagttg gtcccataaa tctagctcta cccttccgca ttcacttgcc
     4741 tgcagccaca cccgaaggga gctctgccac cattccggcc ggaacattgg ccatcgaatc
     4801 gtttcgcttc ggcgctgagc ccattccggg gatcaagaca ccggtctcct tgtggaacaa
     4861 cacgctaaaa tttggtcaac ccattcggct ctccatctat ggcggcacga ttgaaattcg
     4921 caaccttgca tgggccgatt tgatcgaaga tcccctgtcg ttttcacttg ctatcgaggc
     4981 gagagatctc cagctccaaa ggctgacgga aagcttgggt tggtatcgtt tcggcggcac
     5041 cctgagcggc tccattccaa aggtcgagat ggcaggcaac gttctgcgta gcgagggcca
     5101 aatcgagatc ggagtctttg gcgggcaggt gcacgtgagc acgacggaaa tcgaaaatcc
     5161 gttctcctct ttgccggccg tcaagttgaa cgctcgcttt caagatattc atcttgaaca
     5221 agcgtccgaa acctttgcct tcggacgcat ctcgggcatc ttagaaggca ccatcagcga
     5281 tctcatcatt gccaacgggc aaccttcgca gatgcaagcc gacgtgcata ccgtggcgcg
     5341 atcaggcagc agtcagtgga tcagcgtcga agcgctgaac aagatcacgg tgttaagctc
     5401 cggcgaggat ggaagcctga tttatggagg aatcgccagt ttttttgacg aattccgtta
     5461 tagcaagatg gggttcaaag cctccctgag aaacgacaaa ctgactctcc ggggcatcga
     5521 gagcagtgat ggaaaagaat ttcttgtcgt cggcagcttg cttccgccga cagtcaatgt
     5581 catcagccac acacgggaga tcggtttcag tgacctcatg aagcgattgg aacaaatcca
     5641 aaaatcggac aagccgcaga tcaagtgaat atggaggaat catgttgaaa cgtgttgctc
     5701 ttttcatagc gttcttagcc aacgcctgcg taacggtgaa catttatttc ccggccgcag
     5761 cggtggagcg cgcggcggat gagatcgtca aagaaacctg gggcagtcga acggagtcgg
     5821 ctccaagaga gcagcctcgc agtctacttc cggctgcatg gaaaatcgca tccgtcagtt
     5881 tcatcgacga agctttcgcc caagaagccg acatcaatat ttcaaacccc gctattcgcg
     5941 ctttgaaaga ctcgattaaa aatcgcagtg gttcgatcaa gccgtttatg gatcgcggca
     6001 atgtggggct gacaagggat ggcctgctaa cggtccgcac aacagatggc cttaacctca
     6061 aggaacgggc ggaggtgcaa cagctcgtgg acgccgaaaa ccgggaccgt gaatctctgt
     6121 accttgagat agcaaaggca aataatttcc cgaaggaatc cgtaccaaaa atcaaaagca
     6181 tcttcgccaa aagctgggcc gaacaggcgc aatccggttg gtggattcaa gacgcgcaag
     6241 ggaattggca gaagaaatga tccagcccgg ccacgttgcc tattattgtc cctttcccaa
     6301 ctcctgggat cggcgtgtgg cgttgacgac ggcttccatg acggtgccgc gaaatccttt
     6361 ttgctccagt gcgaatagtc ccgcgatcgt cgtgccgccc ggagaggtca cctcgtctct
     6421 cagctcgccg aggtgcttcc cgctttcgag cgccatgcgt gccgagccgt atacagtctg
     6481 cagcgccagc ttgagcgcca catctcggga tagacccact tgaactccgc cgtcggccaa
     6541 tgactcaata aagagaaaaa catacgctgg cccgctcccg ctgagcccgg tcaccgcgtc
     6601 caaatatttt tcctccaccg gcagagcgat cccaaaactg ttgagaatcg agcccaccgt
     6661 ctccatatcc ttcgctgtcg cgtgtgcccc accggcatag cccgatgcgc cggctcccac
     6721 cacgcaggga atattgggca taacccgcac aacgcgagcc ccttggacca aatgggtctc
     6781 gagccgcgaa agaggaacac cggcggcaac cgagaccaaa agtttggctt tgcccagctg
     6841 ctttgcgact tccttgagca ccgcatccac gatctgaggt ttgaccgcaa gaatcacgct
     6901 gtcggcaaaa tcgcagacct tgccgttgtc cggcgtgact tcgatgccga gctcattggc
     6961 aatggattgc cttagcgcgt cgcttacatc gctcgcgatg acttgatctc tagccaccag
     7021 acccacgtta atcattccac gcacgatcgc cgagccgatc tttcccgcgc cgatgattcc
     7081 gattttttcc ttcaacatca cagtgcctcc ctcgcccgtc cctttaaagc tcgttccaga
     7141 ataaagactt gaaagctgaa ctgcaaagca tcccctatca aaaaacaaaa ggccttcggt
     7201 acgtcaccga aggccctctg caacaacacg gaccaaaagg gtttggcgca gctcagtatc
     7261 tcttccctaa ggcttgaaga ccgtgcaagg cttggtcgtc gtcggggctc aaagccaaaa
     7321 cctcgcggta tttctgttcc gcctcccgca ctttgcccag tttcgcgtag gcttcgccca
     7381 gagagcgacg ccaatcgttt cttctccgcc tcattcgcaa cgcttctttg tatgattcga
     7441 cggccttgtc ccacatgttc agtttgacat agacttcccc caactccgaa tagagcgtcg
     7501 cagagtcctc ccctctcccc ttggactttt ccaacagccc cttggcttcc tgaaaaagac
     7561 gggtcgccgc ctctttatcc ttttcacgat tgcctttgac taaggcgagg cgaccgtacg
     7621 ccagcaatac cgccggatgg tccgcttgaa tctggcgcgc aggttcaaac catctttccg
     7681 cttcacggac gtctcccgag tggagcgcga tattccccaa gcccaggtag ggtagaacct
     7741 cacggctgcc caactcgatg gctttggcat agacgatctt ggcttccggc aaatagaact
     7801 cgtcgctcaa ggccaaaata tccgaagtgg tatcccgcga gcgttccatg gccgcgaggg
     7861 cggctttaga cgcttccaca ctcttgtctt gatccaaaag aattttcgtt tgcaagacgt
     7921 agaactttgg attcgtggga tccaaacgta tggcttgctc cagctcgttc aaagcccgat
     7981 tgttggaaac gctcgccacg taggactgcg ccatataata atgatgcatc gcttccggaa
     8041 ccggcagcgg tttgtttttc aaccagggcg gcgaatcaac gatatagggc atcataatct
     8101 tccgattgag ctccgtcgtc gcccggcgca ggctcttggg tgcggagaac tccagttggg
     8161 ccccgtcatc cgtgttgatg tcggcgccct tggaaaaatt caataggttc tctttcccca
     8221 tgcgataaaa accctgcacc gcgtaaacgt ccgataaccc tagatagtcg agatccgact
     8281 tcagcatcca attactgtca tagatttttt tcaccgcagc gtaatcaaac ggatgttcct
     8341 gcttgctgcc gatcagcaaa aaatcgcttt ccttcatgct ccagagcgat acatagggaa
     8401 aagcctcgac gaaagtccga aacaccatgc ggaaatcgtc cggcgacatg gaataattgt
     8461 gaaaccactg cgcgaagatc ccatcgtcct taatcttgga tttgatcacc tggtagaact
     8521 ctctcgtata cagattagcg atgcccgcga tccagggatt cgacggttcg gcggtgatca
     8581 cgtcgtaata ctttggcgtc gcgagaatat agttgcggcc atccgtcgga atcacccgca
     8641 cccgcttgtc ttcgagcacg ctgctatggc gagagttgcg cgcccgccga tgcagcgtat
     8701 cgatggcccc gcgataatcg cgatcctccg aaagtttcat cagtctggac cgttcctcgt
     8761 ccttcatgac gcccttgtag aagagcagtt gcttgctcgg gtcgtaccag acccggcttt
     8821 tcccatcgtc cacggggata ctcactcccg gggggagttt gtccattttc acaaaagccc
     8881 ggtcgaaaaa tttactggct tcgatcatcg ccggctcgat ctcgatgacc tcaatttcct
     8941 tcaaggtgtc gaaggtcgcc aaggcctttg ccgacatgcc gcttcccagg ccgatggtga
     9001 gcgcgcgctc agccttggga tgaagcaaca tgggaatgta gctggtcatg agctggctga
     9061 gggcatcgcc gtaggaaccg tcgattttac cgtttgactt gaagtagatg tattcgctgc
     9121 ccggaatgcg atggacactg actgtagtcg tcagcccttc acggtaaaag agaacgtcgt
     9181 cacgcttcat ttcttccaac cgcagggaat tggtcggcag gccttcgtag cgatcgtgat
     9241 aaatggtgac gccgctggtg agaacatgat gatcccagcg cggcactcga aacgggatta
     9301 agaccgccaa aaccaacacc actaatccca atgcaaatcg cggcaccatg gacatgcgcg
     9361 gatcggccca caccagcagg cagccgatca agagattcat gaccacggca aatacgatgg
     9421 tattttgcac cccaatattc gggatcagaa taaaccctcc ggcaaacgcg cccaaaactg
     9481 cgcccacggt attggcggcg taggagctac caacgccgct gccgacacga tacagacttt
     9541 gagtgaacaa tcgcgctacc aatggaaacg tcatgcccaa gagcaccgtg ggcacgaaca
     9601 tcaccagcgc cgacagaaat atctgcaggt agagaaacac ggtaaacgta tcgccaaagc
     9661 cttgcagcaa gcgaacaaaa atcaagggta gtcgctcgaa aaggggaatg gtcgccagcg
     9721 cggccaagcc tacccagagc tcgatcaacc caaatgtgct cagacgggcc tcccgatttc
     9781 cgagaaaacg cgcgtagatg aatccaccca gagacaaacc gatcaggaaa gtcaccagca
     9841 tcgtggtgaa cgagtagatc gagctgccga tgaccagggt caaagcgcga gtccaggcat
     9901 tctcgtatac taaagaggcg aatcccgaga gcccgaagga gatcaacaga acccaaagca
     9961 gccacgatgg cgcggcttct ccctccgctg ccttggcttc ctccgccacc gtttcggcag
    10021 tgaacaccaa tgattccttg tctcgtatac gatcgacaac caaaatcagc gcggcgataa
    10081 ccagattaac cgtcgccgcg acaaacacag tggcccgcat accgagagcg ggaatgagaa
    10141 gaaacccgcc cgccgcgcaa ccaatcaccg cgcccagcgt gtttgtggcg tagaggtcgc
    10201 ccacgcgcct accgaactgc gcaaagctgc gcacgaagaa gcggctcagc accggcaagg
    10261 tcgcgcccat cagcagagtt ggaaacacga gcagggtaaa agagagcagg aaaagcacca
    10321 tgttgaagag gaaaggatag gactcattga gaccgaaaat cgggccgtag accttttgcg
    10381 ccatacggaa gagccagggt acggcaaatc cgtagatccc cactccggcc tcaagaatgc
    10441 cgtataacag gaagtcgttc ttgccgcggt cggcaatttt tccgaagaga tagctgccaa
    10501 tcgccaatcc ggccatgaaa gcggacaaga ccgtggcgat ggcatacgtc gtattgccga
    10561 agatttgggt gagcatccgg gtccatacga cctggtagat cagacccgcg gcaccggata
    10621 aaaagaagca aaaaagaccg actccaagaa gccaatcttg cttggagcgg tgaaaagttt
    10681 ccgtcatata attcaagaga aacgacctcc gcaagacaat tcaagcacag atagcaagcc
    10741 acattacaac tgtaaagagc cctcgataga cttctaggat cgaacagccg gcgtcacaac
    10801 cgttaacaga ccgacctttc gctgctcaag agtgactatt agacggggct cctacgccat
    10861 agcttcattg acttttgcca gaatgtcaga taagcgtaaa ttccatttgg gcatcccgaa
    10921 tttgccaact ctctcccata tttgtaatcc gattgggctt aggaatttcg aaatttatta
    10981 gagggttgga ctgtttccga cttagccact atagtcaaaa acccagattt tttttggagg
    11041 aaagaaatgg caaaagctgc aaaaagagac ggcgcggcgc ccgaggagat tagggactta
    11101 cgcggagcca tcgaatggat gaagaaagaa ggcgacctca tcataaccga gaaagaggtc
    11161 gaccccaatt tggaaataac cggcatccag aaacaactgg acggtagttg tcccattcta
    11221 ttcaacaatg tcaaaggaaa gccgaaaaat cgctgcatca ccaacctttt tggtgacatg
    11281 aacgtaatca acaaaatgtt cggttggaaa gaccacgctg accgcacgcg aaagctggcc
    11341 tatgctctta cacaccccat tccgcctgtc atcgtcgatc aaaaagacgc cgcggtccag
    11401 gaagaggttt acgagaagcc ggccgaagcc aatgaatttg tcgtgcccat tcgtcacacg
    11461 gacgttgagc ccgagttgac ggtaggttcc ggcaaccgtc tgatctcggg ggagtatttc
    11521 ggcggcggta cggacctcgg ctacaatcgc atgaactttc gttggggcaa cgttggaact
    11581 tttcagatct ctcccggctc acacatgtgg caggtggtca gcaagcacta caaggacaac
    11641 aagcttgtgc ccatcaccat gaactttggt ttgccgccag ccggctcgct actggccggt
    11701 gccggcttcg actatgtcat cctgccgcag ggctgcgatg aagtcggcat cgccggtgca
    11761 gtccaaggcg cgccaatgcg tctggtcaaa gctcgtacgg ttgacgcaat ggcccttgcc
    11821 gacgccgaag tcgttctcga gggttatgtc aatccgcgtg atcgccgctt cgaaaccgcg
    11881 gagtcggaga aggccggcat ccagggccgt ttccactttc atccagaatg ggccggttac
    11941 atgggtaaag cctacaaggc cccgaccttc catgtcaccg ccgttaccac gcgaagacgc
    12001 gactcgaagc cgattatctt caccttgggc gtccacaccc tcgacgacca taacatcgac
    12061 accactgtcc gcgaagccgc gatgttcgag ctttgtgagc ggatgcagcc cggcatcatc
    12121 atggatgtca acattcccta ctgcatgacc gattggggag gggcaatcat ccaggtcaag
    12181 aagcgcaacc gcatcgagga aggctggcag cgcaatttca tggcggctat tctcgcgact
    12241 tcgcaaggtt cgcgactgtg catcgccgtc agcgaagatt gtgatccgta cgatatggac
    12301 gacgtcatgt ggtgtctaac cacccgcgtc aatcctaaga ccgatatcat caatccattg
    12361 cccggcggac gtggacaaac ctttatgcct gccgaacgga tgaccgccgg cgagaaggaa
    12421 tggacggcct ctaacactag tttcgagggc ggcatgggca tcgatgccac cgttccgttc
    12481 ggttacgaga gcgactttat gcgtccggtc tatccggtcg agcgcattga cctgaagaag
    12541 tttttccatg ataaagatat tcaaaatgcc aagtctcgta tgcagggctg ggtccactct
    12601 ttggcccgta ccggaagata agttcggacc agcatcccta attgaaaaaa gggcagggtt
    12661 tgcgtccctg cccttttttt taaaccgcta tggcttctac ggatagaaac gcggggctcc
    12721 tctctcctta ccgagttctc gatcttagta acgagctggg gtttttgtgc ggcaagattt
    12781 taggggatct gggcgcggat gtcattaaga tcgaaccgcc gggaggcgat ccagcacgaa
    12841 gactcggtcc tttttacggc gatcagccgg acccggaaaa gagcctcttc tggttcggct
    12901 tcaacaacaa caagcgcggc atcacgctca atctggaatc cgccaaagga cgcgagctat
    12961 tttctcagct tgttaccaaa gcggatttcg tggttgagac ttgtacacct ggctacctcg
    13021 acagcctaaa tctgggctac aaaatgttgt ccatgatcaa tcctcgcctc gtactcactt
    13081 caatcacgcc ctttggtcaa accggaccct acagcaggtt taaagcctcg gacatcgaga
    13141 tcatggcatt gagcggctgt atgtccctga ctggcgatcc ggatcgtccg ccgctgcgcg
    13201 ttacttttcc acagtcttat ggctggaccg gttcctacgc cgccatggga gcgctgatcg
    13261 cgcaccatta tcgagagcag accggcgagg ggcagcaggt cgacgtctcc gcgcaggctt
    13321 gtttgctctg ggccttttcc catgcccata cattttggga tctcaaccgc cacgtggaaa
    13381 agcgcgcggg ctcattcatg accgggcgaa gcattaccgg tgcgaagatg agggttttct
    13441 ggccctgcca agacggctat ctcaacttca tcatctacgg gggcgaggcc ggcagacgaa
    13501 ccaaccaagc gctggtcgag tggatggaca gcaaaggcat ggcgc
//
LOCUS       DASSWZ010000156         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1193868, whole genome shotgun sequence.
ACCESSION   DASSWZ010000156 DASSWZ010000000
VERSION     DASSWZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193868"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFN58_03845"
     CDS             <1..912
                     /locus_tag="VFN58_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET8917156.1"
                     /translation="SAVSSLLDRCNQNRRLIVLRLVLFVSLMGSSASALEIPALNIPI
                     NDFAKMMPPASIDDLTERLRRMKTQTAYTVAVLTVQSLQDEDIETFGRKVFKNLPLTE
                     KELQRSALLIVARKEQKVGVQTGSELQPLFPQPETNRKLQGQVEIYFNGMRPDLGIHA
                     GVHYILGVIKGDFRVDGASEEEKLEDASKRGAGAGAIFAVLLSPFLAFFVGLLWGVYA
                     THYGVRRETRLFIGAVLGGGTAKIVATLMSMMGSYSDGLWYFILALSIPLGVFGSLTE
                     YWMGGDWSGIPRVKDKIRRKPEDNMGI"
     gene            complement(944..1378)
                     /locus_tag="VFN58_03850"
     CDS             complement(944..1378)
                     /locus_tag="VFN58_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET8917157.1"
                     /translation="MNWIFETAPSFAFVFLRLGLAIAFFFHSTQQIFGWSGGRGMKGM
                     LDNWKDKYGIPVPIGVVGMLTEFLGSFALLVGFLTRPFALGLAIFMWVAMQKAHWEYG
                     FFLARRPGEGSGIEYCLALFLMSMALLIGGGGAFSIDGLLSR"
     gene            complement(1398..1715)
                     /locus_tag="VFN58_03855"
     CDS             complement(1398..1715)
                     /locus_tag="VFN58_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917158.1"
                     /translation="MSIYQVNKLLYLTDNDAAFRKRMKENPEEAIKAFSLSQEEHDAL
                     TSGAVGKLYQMGVHTFLLNHLSRYELFGVNRDNYLSRIREGMEYDPRFEQGQMPMQYF
                     IKE"
     gene            complement(1722..2525)
                     /locus_tag="VFN58_03860"
     CDS             complement(1722..2525)
                     /locus_tag="VFN58_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917159.1"
                     /translation="MAKLVLAAGVPHPPRLVYEMAQSPGKMRGEALMKQVREQVEKAE
                     PDLIIEIDSDHFVNFFYNNLPSFCVGMAEEAQGPQETWCPMPRYTVRGHVPLAKALFK
                     YGLHDKFDLAAAHELRLDHSLVVPLHFLNPSMELPVVPIYTNGFASPLPLATRCFSLG
                     RMVRRFIEAWDGKERVALIASGCFAMDVGGPLRGWTDREWEETISGLLVQGKYQALAR
                     RATEERINKAGNNSGELLNWITLTGALGKTRPLFLENDDGSGYAVWKLE"
     gene            2703..>2989
                     /locus_tag="VFN58_03865"
     CDS             2703..>2989
                     /locus_tag="VFN58_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917160.1"
                     /translation="MAKLRHLAILTHQPGKLADFYKRVFDMKEMYRTKNGSVHLSDGE
                     VNLAILNATDPKEPGHQPGLYHFGFHVEDNKETARRIQEIYPEGAPKEREAT"
BASE COUNT          730 a          838 c          795 g          626 t
ORIGIN      
        1 tccgcggtga gttctcttct ggatcgatgc aatcagaata ggcgactgat agttctcagg
       61 ctagttcttt ttgtcagcct gatgggctct tccgcgagtg ccttggaaat ccccgcgctc
      121 aacattccca tcaacgactt cgccaagatg atgccgccgg cgagcatcga cgatttgacc
      181 gaacgcttga ggcggatgaa aactcagacc gcctacaccg ttgcggttct tacggtacaa
      241 agcctgcaag atgaagacat cgaaaccttt ggacgcaaag tctttaagaa tctcccgttg
      301 acggagaagg aattacaaag gtcggccctg cttatcgtcg ctcgcaagga acaaaaagtc
      361 ggggttcaga ccggatcgga gctgcaaccc ctttttcccc aacctgaaac caaccggaaa
      421 ctccaaggac aggtggaaat ttattttaac ggcatgaggc ccgatctggg gattcacgcg
      481 ggagtgcact atattctcgg ggtgatcaaa ggggatttcc gcgttgacgg cgcatcggag
      541 gaggaaaaac ttgaagatgc ctcgaagcga ggcgcagggg cgggagcgat cttcgcagta
      601 ttgttgtcgc cctttctggc attttttgtc gggctgttat ggggcgtcta cgccacacat
      661 tacggcgttc gccgtgaaac ccggcttttt atcggcgccg tgctgggagg cggaacggca
      721 aagatcgtcg cgactcttat gtcgatgatg ggtagctata gcgacggcct ctggtacttt
      781 attctggctc tcagcattcc tctaggtgtt tttggcagtc tgacagagta ctggatggga
      841 ggagattgga gcggcatacc gcgtgtaaag gataaaataa gacggaagcc tgaagataat
      901 atggggatct gacagcacgc cgcggaagga ttcgagtttc gctttaacgg ctcagcagtc
      961 cgtcgatcga gaaagcgcct cccccgccaa taagcaaagc catcgacatc agaaaaagcg
     1021 ccaagcagta ctcgatcccg ctgccctctc ccggacggcg cgccagaaag aatccgtact
     1081 cccagtgagc cttctgcatc gccacccaca tgaaaatcgc caaccctagg gcaaacggcc
     1141 gggtgagaaa cccgaccaga agggcgaagc ttcccaggaa ttcggtaagc attccaacca
     1201 cgccaatggg caccggtatt ccgtacttgt ccttccagtt atcgagcatg cctttcattc
     1261 cgcgtccgcc tgaccaaccg aaaatctgct gcgtcgagtg gaagaagaag gcgatcgcca
     1321 agcccaggcg taaaaagaca aaagcaaaag atggggctgt ctcaaagatc cagttcattc
     1381 cgaacctccg tggagtctca ttctttgata aaatactgca tgggcatctg gccttgctcg
     1441 aagcgcggat cgtattccat cccttcacga atccgagaaa gatagttgtc tcggttcacg
     1501 ccaaacagct catagcggga aagatgattg agcaaaaacg tgtggacacc catctgataa
     1561 agtttgccga ccgcgcctga agtcagggca tcatgctctt cctggctcag ggaaaaggct
     1621 tttatcgcct cttcagggtt ttctttcatt cgtttgcgaa aagcagcgtc gttgtccgtc
     1681 aaatagagca gcttgttgac ctgatagata ctcatctgtc tctactccaa tttccatacg
     1741 gcgtagccgg agccgtcgtc gttttccaaa aacaggggcc gggtctttcc caacgcgccg
     1801 gtgagggtaa tccagttcag cagctcgccg ctgttgttcc cggccttatt gatgcgctct
     1861 tcggtggcgc gacgggccag cgcctggtat ttcccttgaa ccagcagacc ggagattgtt
     1921 tcctcccact ccctgtcggt ccaaccccgg agcggcccgc cgacgtccat agcgaaacaa
     1981 ccgctagcga tcagggccac tcgctcctta ccgtcccatg cctcgataaa acggcgcacc
     2041 atacggccaa gggaaaaaca gcgcgtcgcc agcggcaagg gcgaagcaaa accattggtg
     2101 tagatgggaa cgacgggcag ttccatcgac ggattgagaa aatgcaacgg cacgaccaac
     2161 gagtgatcca gacgcagctc atgagcggcc gcgagatcga acttgtcatg gagcccgtat
     2221 ttgaacagag ccttggccaa aggaacatgc ccgcgcaccg tatagcgagg catcgggcac
     2281 caggtctcct gagggccctg ggcttcctcg gccataccga cgcaaaaaga cggcagattg
     2341 ttatagaaaa aattcacaaa atgatcgctg tcgatctcga tgatcagatc aggttcggcc
     2401 ttttccactt gttcgcgcac ttgcttcatc agcgcttctc cccgcatttt tcccggcgac
     2461 tgggccatct cgtagaccag ccggggcgga tgtggaactc cagcggccaa cactagtttt
     2521 gccatgcagt tctcctctcg ggtgttggtt caacacgacg ctcgccgacc ctcttaacat
     2581 tgcgccaaac cccgcgtcaa tatccgatga gattgccgac gaaacgccgc ggccgttgac
     2641 agcgaaaagc gccggtgata gcgctgactt tcaattgcaa aaaatcacag ggaggaaaga
     2701 caatggccaa actccgtcat ctcgcgattc tcactcatca gccgggcaag ctggctgatt
     2761 tctacaagag agtgttcgac atgaaagaga tgtaccgcac caagaacggc tcggtccatc
     2821 tttctgacgg cgaagtgaac ctggcgatcc tgaacgccac cgatccgaag gaaccgggac
     2881 atcagccggg cctctatcac ttcggctttc acgtcgaaga caacaaagaa accgcgagac
     2941 gcattcagga aatctatccg gagggcgcac cgaaagagcg cgaagccac
//
LOCUS       DASSWZ010000157         4227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1363482, whole genome shotgun sequence.
ACCESSION   DASSWZ010000157 DASSWZ010000000
VERSION     DASSWZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363482"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2727
                     /locus_tag="VFN58_03870"
     CDS             <1..2727
                     /locus_tag="VFN58_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET8917161.1"
                     /translation="VEILLQSGSSGKPDIRLARTSHMRKSHKLLLFFLIASAAAVMAF
                     IQTFNTLAAKNREQVHQELQRFLGKDATFDRLEASLWGGLGFSAKEFRIADDPRFAAT
                     PVIHAQELRLGVSLLPLLLGKIVINSLTFQAPEFQIITDERGLLNLAELAGQKNETKP
                     LPRSQTSSPERKPLTVNFLVAKISVKNGRVDFIDRSIKEPAEIRVKNVEMEVKGLNPS
                     EKTRIKFAAAVTEGLGHDVRIDGALGPLRQPHGWSQQPVELEIRFDSLSATLLARALP
                     FLRNRIPRELDVTGPLSLQAKLGGTFNRPRITDIALKIPLFGSSNYNAIAEGSLEIPE
                     GRPWAEAQLKGKLTLTSINLTQLRNLPFLKQTLPIALETEGSINAYSQFEGTWANLRV
                     GALIKGEKSEFRYRDWFRKPAGSPAELKAKISRQKIGLVLHDSELSFGNSKMTLSGVV
                     EEALQPRLQLKLRSDSSHLPTWWRLVSPLSFDGVGGTIGWDIVLRKNYASGDGWNIHG
                     KLNLADAEFRHKQTGRKIDQLNAAIAFLGTEALLEKGSFRLGSSLITVAAKVTDLTQP
                     SARYQLRSPEVHLMDLQTFLAGKTNQIRNVTASGEIRWQDDAPSLRGTLSSSEGNLED
                     IPYRELQADVTWSPKGISFKNLSLQAFDGVLRADGYWPAGVDPAQRFSLKSQSESIQL
                     GSLLKQKFPQLKNRLEGQLNFRGQFDAATQNGATVTKSLQGSGESVIHHGTIRDFNLI
                     AKIIPGGGGDSGSSRLPANLAALVDRPYTPFDTLKANFRVDGQRIRTDDLLLVTPDYT
                     ITGAGWVGFDRTTQWNGLLVFSPRITQELQREYKMIRYLLDRRGRLSISFRAEGRFPN
                     VKVKPENRAVAQVLRRSFPQKADEPAAGDGKSTEKNERKTWLPESLEQLLKQ"
     gene            2740..3009
                     /locus_tag="VFN58_03875"
     CDS             2740..3009
                     /locus_tag="VFN58_03875"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HET8917162.1"
                     /translation="MELREIYLDTFPEHIAYVKFIFESYEEVGIIRTVDRKKAIIVLL
                     AMDDFLDTARSILTSIQRDVPLLEIPRPADMGDDWFMTELAVESS"
     gene            3154..>4227
                     /locus_tag="VFN58_03880"
     CDS             3154..>4227
                     /locus_tag="VFN58_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET8917163.1"
                     /translation="MSKNSQATAPAPGRTQQRILDLFAKKPRTRLTPVEIQKRAGFGR
                     DELQRIVDALRELTREGRIVRLKKNHYALPDAQNLVTGRVHAHPDGFGFLILDDKGAE
                     DLYLNRREMRRVMHGDRVMVRADRKKRGGVEAHVVQMLERAQKRLIGTYQELDGRGYL
                     IPMDPRIAAAIPLRPSGSKPEKGKVVAAEISRYGTAMSGPEAELVQVIGDPDDPEVQV
                     QSIVFRYGLTTNFPEPVHREIASASFSIREDEIVARTDLRNLPIVTIDGELARDFDDA
                     VCVRKTNGHHELLVSIADVAFYVRPGTAIDQEAYNRATSVYFPDRAIPMLPEALSNGI
                     CSLNPNEDRLTKTVAMEINGKGEV"
BASE COUNT         1058 a         1144 c         1112 g          913 t
ORIGIN      
        1 gtcgaaatac ttcttcaatc gggtagttct gggaaaccgg atatcaggct tgcacgaacc
       61 agccacatga gaaaatcgca caaattgctg ttgttttttc tgattgcgtc tgcagcggcg
      121 gtaatggctt ttattcagac cttcaacaca ctggcggcca agaaccgtga acaggtgcat
      181 caggaactac aaagatttct gggcaaggac gccaccttcg atcgtctcga ggccagcttg
      241 tggggtggac tcggcttctc tgcgaaagaa tttcgcattg ctgacgatcc tcgttttgcc
      301 gccaccccag taatccacgc ccaagaatta aggctggggg taagcttgct cccgctattg
      361 ctcggcaaaa ttgtcatcaa ctcactgacc ttccaagcgc cggaattcca aatcatcacg
      421 gacgaacgag gtctattaaa cctggcagag ctggccggcc aaaagaatga aaccaaaccc
      481 ttgcccagat cacaaacgtc ttcaccggaa agaaaaccac ttacagtaaa ttttttagtg
      541 gcaaaaatta gtgtaaagaa cggccgggtt gatttcatcg accgctccat caaggagccc
      601 gcggagattc gcgtcaaaaa cgtagagatg gaagtcaaag gactcaaccc ttcggaaaag
      661 accaggatta aattcgctgc ggcggtaacc gagggactcg gtcacgatgt caggatcgat
      721 ggagcgctgg gcccgcttcg gcaaccgcat ggttggtcgc agcaacccgt ggagcttgaa
      781 atacgattcg actctctgtc cgctacgctg ctcgcccgcg ccttaccctt tcttagaaac
      841 aggattcccc gcgagctgga tgtaaccggc ccgttgtcgc tccaggccaa actcggaggc
      901 actttcaacc ggcctcgaat cacagacatt gccctcaaga tccctttgtt cggctcttca
      961 aactacaacg ccattgcgga gggttccctg gaaattcccg aaggccgtcc ctgggcagag
     1021 gcgcagctca aaggaaaact gaccttgacc tcgatcaatc tgacgcaact tcgtaacctg
     1081 ccttttctca aacagactct tccaatagcg ctagagacgg aaggttccat aaatgcctat
     1141 agtcagttcg aaggaacctg ggctaacttg cgcgtgggcg ctttgatcaa gggggagaaa
     1201 agcgaatttc gctacaggga ttggttccgc aagcccgccg gtagcccggc tgaactcaaa
     1261 gctaaaatct cgcggcaaaa aattggactg gtgcttcacg actcggagct aagtttcggc
     1321 aattcgaaaa tgacgctttc aggtgttgtt gaagaagcgc tgcagcctcg cctgcaactt
     1381 aagctgcgta gtgattcaag ccatttgcca acgtggtggc gcctggtttc tccgctgtcg
     1441 tttgatggcg tcggtggaac cattggttgg gacattgtat tgcggaagaa ctatgcttca
     1501 ggggacggct ggaatatcca tgggaaactg aatctcgccg acgccgagtt caggcacaag
     1561 caaaccggaa gaaagatcga ccagctcaac gcagctattg cgtttctagg aacagaggct
     1621 cttttggaaa aaggttcttt tcgtttaggt tcgtccctga ttacggtcgc agccaaagtt
     1681 acagacctaa ctcaaccaag cgctcgctac caattacggt cgccggaagt ccaccttatg
     1741 gatctgcaaa cgtttcttgc cggtaaaaca aaccagattc gaaacgtgac agccagcggc
     1801 gaaattcgct ggcaggacga cgctccctcg ctgaggggaa ccctttcgtc ttccgaaggg
     1861 aatctggaag atatccctta ccgcgaacta caagcagacg taacgtggtc gccaaagggt
     1921 ataagtttca aaaatctttc gttacaggct ttcgacgggg tgcttcgcgc tgacggttac
     1981 tggcccgctg gtgtcgaccc ggcgcaacgc ttttcgttga agtcgcaatc tgaatccata
     2041 caattgggat cccttctcaa gcaaaaattt cctcagttaa aaaatcgcct cgaaggacaa
     2101 ctgaattttc gcggtcaatt cgacgccgca acccaaaatg gggcgaccgt cacaaaatcg
     2161 cttcaaggat cgggcgagag cgtcattcat cacggcacaa ttcgagactt caatttgatt
     2221 gcgaagatca tccccggcgg gggcggcgat tccgggtcat ctcgcctccc ggccaatctc
     2281 gcggccttag tggatcggcc ctatacgccg tttgacactc tcaaagccaa ctttagggtt
     2341 gatggacaac ggattcgcac cgacgatttg ttactcgtga cgccggacta cacaatcacc
     2401 ggagcgggct gggtcgggtt tgacagaacg actcaatgga acggattgct ggttttttcg
     2461 cctcggatca cgcaagaact tcagcgcgag tataaaatga tccgttattt gctcgatcga
     2521 cgcggcaggc tgtccatctc tttccgcgca gaaggtagat ttccaaacgt caaggtgaaa
     2581 ccggaaaacc gagcggtggc ccaagtctta cgccgcagtt ttccgcaaaa ggccgacgag
     2641 cccgccgcgg gcgatggcaa gagcacagag aagaatgaaa ggaaaacttg gcttcccgag
     2701 tcactcgagc aattgctcaa gcaatgatct tctaatgcta tggaattacg agaaatttat
     2761 ctcgatacct ttcctgaaca catcgcctac gtgaagttca tctttgagtc ctacgaagaa
     2821 gttggaatta tccgaaccgt cgaccgaaag aaagccatca tcgtgctgct ggccatggat
     2881 gactttctag acacggcacg gagcattttg acctcgatcc agcgggatgt gccgctcctg
     2941 gaaattcccc ggcccgccga tatgggtgat gactggttca tgacagagct cgcggtggag
     3001 tcctcgtagg gctcatggaa tattggaata atggagcggt ggaatacggg agcgctgggg
     3061 tgtgaaagca tttcctgttt taatccatca ctccaggact ccaacatctc gcggccgcaa
     3121 acccttaaca aaagagtagc tgctagacct cccatgagca agaattccca agccacagct
     3181 ccggccccag gacgcacgca gcaaagaatt ctcgatctct tcgccaaaaa gccgagaacg
     3241 cgattgacgc ccgtcgaaat acagaaacgc gcgggatttg ggcgcgacga acttcagcgg
     3301 attgtcgacg ccttgcggga actcactcgt gaaggtcgaa tcgttcgctt gaagaaaaat
     3361 cactacgcgc tgcccgatgc acaaaacttg gtaacgggac gggtccacgc ccacccggac
     3421 ggcttcggtt ttctcattct cgacgataag ggtgcagagg atctttacct caatcgccgc
     3481 gaaatgcgcc gcgtgatgca cggagaccgg gtcatggtcc gggccgaccg aaagaagcgg
     3541 ggcggcgtcg aagctcacgt cgtgcaaatg ctggagcggg cacagaaacg gctcatcggg
     3601 acctatcagg aactagatgg cagaggctat ctgattccga tggatccgcg aatcgccgct
     3661 gccattccgt tgcggccaag cgggtccaag cccgaaaagg gaaaagtggt cgccgcggag
     3721 atctcccgat acgggacggc tatgtcggga ccagaggcag aactcgtaca ggtcatcggg
     3781 gatcctgatg atccggaggt tcaggttcag tcgatcgttt tccgttacgg tcttacaacc
     3841 aattttccag aaccagtcca tcgggaaatc gcaagcgcct cattctccat cagggaggat
     3901 gagattgtcg cgcgaaccga tctcagaaat ttaccgatcg ttaccattga cggcgagctg
     3961 gcgcgcgact ttgacgacgc cgtatgcgtg cgaaaaacca acggccacca cgagcttttg
     4021 gtctctattg ccgacgttgc tttctacgtt cgaccgggaa ccgccatcga tcaagaggct
     4081 tacaaccggg ctacgagcgt ctattttccg gatagagcca tccccatgct tcctgaggct
     4141 ctctccaatg gtatctgcag cttgaatccc aacgaggatc ggctcaccaa aaccgtggcc
     4201 atggaaatca acggcaaagg cgaagtc
//
LOCUS       DASSWZ010000158         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_245687, whole genome shotgun sequence.
ACCESSION   DASSWZ010000158 DASSWZ010000000
VERSION     DASSWZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245687"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VFN58_03885"
     CDS             <1..134
                     /locus_tag="VFN58_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET8917164.1"
                     /translation="RGERFGLIMFGSRTDTYLPEGCQVAVTEGQRVKGGETVLARFV"
     gene            131..988
                     /gene="pssA"
                     /locus_tag="VFN58_03890"
     CDS             131..988
                     /gene="pssA"
                     /locus_tag="VFN58_03890"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET8917165.1"
                     /translation="MSGINPPEPEQFPAVRNVRLLRRRVRPRVPLRNRVAAVKLRKGV
                     YLIPSLFTAGNLICGFFSIISTFNGDYVPATLFIILANILDGLDGYAARLTRSTSQFG
                     VEFDSLADVVSFGVAPAILVYFWALVPWGTWGWLAACTFVVCGALRLSRFNVQSTGPA
                     KGHFVGLPIPAAAEMIASIVLMYYFLGGEGAPNKHVTLLLVIYGLAGLMVSSFPYFSL
                     KNSALRKKHPFWMLVSGFILITLIIAEPQIMFFMIFLLYTLSGPLLWCLTTSKRRRER
                     RGQMAEANP"
     gene            1082..>2560
                     /locus_tag="VFN58_03895"
     CDS             1082..>2560
                     /locus_tag="VFN58_03895"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET8917166.1"
                     /translation="MSESVADNIVKIFDTTLRDGEQSPGASMTVEQKVVIARQLEKLG
                     VDVIEAGFAASSEGDFESVARVCKEVKRPRVLSLARSQEGDITRALKAVEHAKNPGIH
                     VFIATSDIHLKHKLRMSREQVLEAAVKAVSFAKKHIDYIEFSAEDASRSDPDFLMQVF
                     KEVIRAGAKTINVPDTTGYAIPSEFGALMANLIERTAGGDRVTWSAHCHNDLGLAVAN
                     SLAAVHNGARQIECTINGIGERAGNTSLEEVVMALRTRKNYLDLDTGITTEQIYPTSR
                     LVSQVTGIPVAINKPIVGDNAFAHEAGIHQDGVLKHKQTYEIMTPESVGITGNRLVMG
                     KHSGRHAFDERLKHLGFNLSKEDMNRAFLRFKELADKKKNVYDEDIEAIVGEEILRVP
                     GQPDRYELLYLNVNSSSDGIPAATIKMRVNGQEKMDYASGDGAVDACYKVITKITGSQ
                     SQLVRYTVSAITGGTDAQGEVSCIIEDGGFRVSGQGAHTDIIM"
BASE COUNT          568 a          643 c          756 g          593 t
ORIGIN      
        1 cccgtggtga aagatttgga ctgatcatgt tcggctccag aacggacacc tacctgccgg
       61 agggttgcca ggttgcagtc accgaagggc aaagagttaa gggcggagag accgtattag
      121 cgaggtttgt atgagtggaa tcaatccacc agaaccggaa cagtttccgg ccgtaaggaa
      181 tgttcgattg ctacgccggc gggtacgacc gcgggtaccg cttcgtaaca gagtagccgc
      241 tgtaaagctt aggaagggag tctatctgat tcccagtctc tttaccgcgg gcaatttgat
      301 ctgcggcttt ttttccatca tttcaacttt taacggggac tatgtgccgg cgacgctttt
      361 catcatcctg gccaacattc tcgacgggct ggatggctac gcggcgcgtt tgacgcgatc
      421 caccagtcaa ttcggtgttg agttcgactc cttggccgac gttgtttcgt tcggtgtggc
      481 tccggcaatt ctcgtatact tttgggcgct ggtgccttgg ggaacttggg gctggttggc
      541 ggcttgtacc tttgttgtgt gtggcgcgct gcggttgtct cgcttcaatg ttcagagtac
      601 aggtcccgcc aaaggtcatt tcgtcgggct gcccataccc gcggctgcgg agatgattgc
      661 gtccattgtg ctgatgtatt actttttagg cggagaaggg gcgcccaaca agcacgttac
      721 gttactgttg gttatttatg gattggcggg gttgatggtg agcagtttcc cgtattttag
      781 cttgaaaaat tcggcgttgc ggaaaaaaca ccccttctgg atgttggttt ccggttttat
      841 tttgattaca ctgataatcg ccgagcctca gattatgttc tttatgatat tcctgttata
      901 caccttatcg ggccccctgt tatggtgttt gacgacaagc aaacgccgac gggagagacg
      961 agggcagatg gctgaagcta acccctgatt cccccatctg aactcgcaac cgatactccc
     1021 gttggccacg ccgcgggagt ttttttttag ccagggccta aggaatgatt ggaggacgtc
     1081 catgtcagaa agcgtagcag acaatattgt gaaaattttc gatacgacct tgcgggacgg
     1141 cgagcagtcg ccgggagcca gcatgacggt tgaacaaaaa gtagtcatcg cgcgccagct
     1201 ggagaaactc ggtgtcgatg tcattgaggc cggcttcgcg gcatcatctg agggtgattt
     1261 cgagtcggtg gcacgggtat gtaaggaagt gaagcgaccg cgtgtcctca gcctcgcccg
     1321 ctcccaagag ggagatatca cccgagcgct caaggcggtc gaacatgcca aaaatccagg
     1381 catccatgtg ttcatcgcaa cctctgatat tcacctgaag cacaaactca ggatgtcgcg
     1441 agaacaggtc ctggaggcgg ccgtgaaggc ggttagcttt gccaagaagc acatcgacta
     1501 catcgaattc tcagccgaag acgcctcgcg cagcgacccg gattttctca tgcaggtttt
     1561 caaggaggtt attcgcgccg gcgccaaaac gatcaatgtg ccggatacga caggctacgc
     1621 gatcccgtcg gagttcggtg ccttgatggc caacctgatc gagcggaccg cgggagggga
     1681 tcgagtgacc tggagcgctc actgtcacaa cgatttgggg ctcgccgtcg ccaattcctt
     1741 ggccgcggtt cacaatggtg cccggcaaat cgagtgtacg atcaacggga tcggcgagcg
     1801 cgctggtaat acctccttgg aagaggtggt gatggcgctc cgaaccagga agaactatct
     1861 cgatttggat accggtatca cgaccgaaca gatctatccg acttcacgtt tggtgtcgca
     1921 ggtgacggga attccagtgg ccatcaacaa gccgatcgtc ggagataacg ccttcgcgca
     1981 tgaggcgggc attcatcaag acggagtgct caagcataag cagacctatg aaatcatgac
     2041 gcccgagtcc gtcggcatca ccggtaaccg tctggtgatg ggcaagcact cggggcgcca
     2101 cgccttcgat gagcggctaa agcacttggg ttttaatctg agcaaggaag acatgaaccg
     2161 ggcgttcctg cgctttaaag agttggcgga caagaaaaag aacgtttatg acgaggatat
     2221 cgaagcgatc gtcggggaag aaattctccg ggtgcccggg caacctgacc gctacgagct
     2281 gttgtatctc aacgtcaatt ccagctcgga cggcatccct gcagcaacga tcaagatgcg
     2341 tgtcaacggt caggaaaaga tggattacgc ctctggcgat ggcgccgtgg acgcttgtta
     2401 caaagtgatt accaagatca ccggctctca atcccagctc gttcgttata cggtgagcgc
     2461 catcacgggc ggaaccgatg cccagggaga agtttcgtgc atcattgagg acggcggttt
     2521 tcgagtgtcg gggcagggcg cgcatacgga tatcatcatg
//
LOCUS       DASSWZ010000159         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_854358, whole genome shotgun sequence.
ACCESSION   DASSWZ010000159 DASSWZ010000000
VERSION     DASSWZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854358"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            668..1354
                     /locus_tag="VFN58_03900"
     CDS             668..1354
                     /locus_tag="VFN58_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET8917167.1"
                     /translation="MATKDYYLILGVSRRENLRSVQEAFRELAKRYHPDRAGPQGTRI
                     FQDLREAYEVLSNPEKRKLYNHKLEQDEIKTHSRPEPIFSHPPSRPEALVPQPMSLLH
                     DFETIRPSFEPLFERFLRNFTGERVPKSEQLESLNVEVILSPEEAARGVVVPIAVPVF
                     YTCPQCGGSGRDWLFPCIDCNAQGIFEEEATVSFRIPPMTPDRAVIEMPIHGLGIHNF
                     YLRLHIRISR"
     gene            1468..1644
                     /locus_tag="VFN58_03905"
     CDS             1468..1644
                     /locus_tag="VFN58_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET8917168.1"
                     /translation="MKDGDNVRYAGLVICRRRPGTAGGVVFMTLEDETGFVNAVIWES
                     VFQRYSVLAKTMSF"
BASE COUNT          500 a          593 c          612 g          544 t
ORIGIN      
        1 gttgacagtc acttcgcaca gtggtattgc gatgctgtcg aagggatcac agcccatcca
       61 tccttgatag actttccagc tcggacagag cttcatcatc tccgatagag caagcttagg
      121 ttagtggtct ttgagaggaa cgaaatgtgt tcctcgggcg aagataaatg agtcgtcaaa
      181 gccggtcgca cttgcggttg tgcggcaaga gtgtaccgtc cggactccat cagcaagcca
      241 tcccctatcg gacaaggagc taagctaaga gcatgcgcca aagcctacat gaaccgcgcc
      301 gaagaactct tcagccggat cgaataggag ccaaaaatgg acatgcagcc tggcgtctgc
      361 gtaattgatt tatttggggt gagctatgac gggactttgc cgagtattgt gtttgtctca
      421 gatgaggttt ggttcggcat ttttgcggtt gcaccgccaa atcctcccga cctagagtct
      481 gccagtcgtc cggagcggcg gcattgtctc tctcttgaaa gccgtgagcg tgtcctccga
      541 tgcttcgggt gaccgttcgc acttgtcagt gagagaggtt gccgacgcgg cagaacgggc
      601 ttcgcttagg agtgagaagg cagcctaaca gaacggatca cagaagctga aaacgcggcc
      661 gccgcctatg gcgacaaagg attactatct gattcttggc gtttcacgcc gggaaaattt
      721 gcgcagcgtg caggaggcct ttcgggaatt ggccaagaga taccaccccg atcgggccgg
      781 tccccaagga acccgtatat tccaagactt acgggaagcc tacgaagttc tatccaatcc
      841 cgaaaaaagg aaactctaca accataagct tgagcaagac gaaataaaaa cacattcgcg
      901 gccggaaccg atcttttccc accccccatc caggccggag gcgctggtcc cgcagcctat
      961 gtcgctgtta catgactttg aaaccatccg tccatcattt gagccgttgt ttgagcgctt
     1021 tctgagaaac tttacagggg aaagagttcc gaaaagtgag cagctcgaaa gtttaaatgt
     1081 tgaagtgatt ctttctcctg aggaagcggc cagaggtgtc gttgttccca tcgccgttcc
     1141 ggtgttttat acctgtcccc agtgcggcgg ttcgggacgt gattggcttt ttccctgtat
     1201 tgattgcaat gcccaaggaa tatttgaaga agaggcaacg gtcagcttcc ggattccgcc
     1261 catgactccc gacagagccg tcattgaaat gcccatccac ggattgggta tccataattt
     1321 ttatctccgt ctccacatcc ggatttcgcg gtaagcgcgg gcccctggca tctcacgact
     1381 tcgcacagcc tgcgccgcca cccgctcgaa ccgatgcggg caagccttgc tcaacaaggc
     1441 ttaccggatg cccgtacggt gacaatcatg aaggatggcg acaatgtccg ctacgccggc
     1501 ctggtcattt gccggcggcg ccctggaacc gccggcggag ttgttttcat gacgctggaa
     1561 gatgaaacgg ggttcgtaaa cgctgtgata tgggaaagcg tatttcagcg ctattctgtt
     1621 ttggcgaaga ccatgagctt ttaggaatca ccggaactct ccaggttaag atggtgtcgt
     1681 tcatattgtt gctgaaaagc tttggcgccc gacgctggaa ctcaaaccga gtggcacagc
     1741 cacccgggat tttcattgag agcaattctc ttcgtaaacc gccagcgttt tttccgcgtg
     1801 aaattgatat gattggagtc gtttagtgct gtgattatag gagtcgttat ttgtggacaa
     1861 gtgacgtgaa tacgtgaccg tgaggtgagt ggtcgtcgac agcccctctc gtaggatagc
     1921 ttgcggagag accaatgcca tgaagcctta tgaattgatc cggtcagcgc tactcggaaa
     1981 ccgctcgtgg gagcaatttt cgatcctgct cgcccgcgtt tcgctcggaa cgtttttcgc
     2041 catttcggga ggtaacaagc tcttcgtcgc cagccacacc cgccggatgt atgaaaccct
     2101 tgccggcgcc ggaataccct ttccgcattt catgacttat ttcgctcctc cgtcgagttc
     2161 atcagcggct ggctgctcgt catcgggttg ctttcgagcc tgtgctgcgc ggctcttatc
     2221 atccagatga gcgtcgcgat cacgacggt
//
LOCUS       DASSWZ010000160        10520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_542970, whole genome shotgun sequence.
ACCESSION   DASSWZ010000160 DASSWZ010000000
VERSION     DASSWZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542970"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFN58_03910"
     CDS             <1..191
                     /locus_tag="VFN58_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate dehydrogenase"
                     /protein_id="HET8917169.1"
                     /translation="DVISREPPPADHPIIRAAKELDNLLVTPHTAWSAREARERLLGE
                     VEGNIRAFFEGKPRNIVV"
     gene            complement(231..653)
                     /gene="ssb"
                     /locus_tag="VFN58_03915"
     CDS             complement(231..653)
                     /gene="ssb"
                     /locus_tag="VFN58_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET8917170.1"
                     /translation="MSVNKAILIGNLGKDPEVRFTSTGRAVARFPIATSEVWTDAEGN
                     RQERTEWHNIIVWGKQGETCGQYLAKGRQVFVEGSIRTRSYDDKSGNKRYMTEIVAQR
                     VRFLGGGGGTRIAPETESGASDEMAMGGPPPADDDIPF"
     gene            890..1672
                     /locus_tag="VFN58_03920"
     CDS             890..1672
                     /locus_tag="VFN58_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET8917171.1"
                     /translation="MLYSFKLLVISVVTVPAALLIILLGVFDRYGKYVYGITRLWTWM
                     ILMIGGVGLKVTGLSHIDPKRQYIFMVNHQSHIDIPVLIQSLPSFQLRWIAKRELLWV
                     PFFGWAMWAAKHITVDRSDRMDALGSLKKAKERMKGGISLVIFPEGTRSSDGNLLPFK
                     RGGLLLAVRTQTPIVPVTISGSWAILPKGDWRIRGGQIEVTVGAPVSVKNHRPGTLRE
                     LSVHVQELIENNLQTAAQIKTSKSGEEQSTTAVRSSMEKRTV"
     gene            1680..3335
                     /locus_tag="VFN58_03925"
     CDS             1680..3335
                     /locus_tag="VFN58_03925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET8917172.1"
                     /translation="MEPLRIIPLGGLGEFGLNMMLLEYGDAAIAIDCGLMFPGADLLG
                     IDLVIPDVSYLLEKKDSLKGIVLSHAHEDHVGALPYILSRLSVPIFGTRLTLGLLNNK
                     LREHSLDNTADIREITAGKPWEIAPFQVEGIRVTHSLMDCLALAIETPVGTVIHTGDF
                     KIDNTPMEGEMFDFQRFGAYGEKGVLLLLSDSTNVERSGYTPSEREVGRNLEQIFQES
                     RGKILVSTFSSSIPRIQQVVDISESCGRRVVLSGRSMIRNSQVAAELGYLRLPRSFMT
                     ENEPWHELPAERLTFLTTGSQGEPLSVLHRVALDDHKSIKVDPGDTVILSSKFIPGNE
                     KTISNLINHLYRRGAEVHYEKVSEIHVSGHASQEELKTMLQLTRPRYFVPIHGEYRHL
                     VKHRKLAQDVGIPQDHCFVLEDGDVLEISATSAKKVQPIQAGRVFVDGKGVGDVGDVV
                     IRDRRHLSEDGMVLAVMAIHQQSGEIVAGPDLISRGFMRDEEGEEVLGQARKVVLDIL
                     ASLNRETRTDPAELQEEVRKALRRYFRKRFERHPVVLPYIIET"
     gene            3364..5607
                     /locus_tag="VFN58_03930"
     CDS             3364..5607
                     /locus_tag="VFN58_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET8917173.1"
                     /translation="MEKGRLNREIWGVVICLAALLIIISLFTYLPSDRSFNTPSGAID
                     TNNWGGFVGAYLADLLLQGLGMAAYLLPIFLCLIALQMFRANYTRIPYSKVIGYAVLL
                     ISTAVILCLFIESENAREAGGIVGGFLKETVLIPLFGRISAVLISLFALILSLMLLTQ
                     NSLLDILNSAKKQLSALKKLLVPAVLNRLRELKEKKEKLKGENTKKERRDYVPPPIVL
                     KEDHKEDPARKIQKKTAISPEQFKLPEIGEGYKLPPPELLDLPEEVNLKLDTETLHAN
                     SLILQKKLADFDVEGEVVAVRPGPVITMYEFKPAPGVKVRRIVTLADDLAMALRAVSV
                     RILAPIPGESVVGIEIPNPRREIVFLREVIESEAYQRADSKLTLALGKDISGSPFATD
                     LARMPHLLVAGATGTGKSVSINAMILSILFKSTPQDVQFIMVDPKMLELTIYEDIPHL
                     LVPVVTDPKKAAAALFWAMDEMDHRYRLMRDKGARNIDNYNRVLEKETATKKGVIELM
                     EGDPNHATSEFGGDLDKAPPLVHERLPRIVIIIDELADLMMSVGRDIEEYITRLAQKA
                     RAAGIHLILATQRPSVDVITGLIKANFPARISFQVTSRVDSRTILDSIGSEKLLGNGD
                     LLFLPPGTARLTRLHGAFVSDQEVRKVMKFIKQQASPRYRPEVFEVKREVEAAAVDEE
                     YDEMYDQAVAVVTETQQASISMIQRRLRVGYNRAARMIEQMEREGVVGPPDGAKPREV
                     YARKLEV"
     gene            5585..6271
                     /locus_tag="VFN58_03935"
     CDS             5585..6271
                     /locus_tag="VFN58_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET8917174.1"
                     /translation="MLESSKFRTSPKILSRLIFALAVTITSAESSATENRSGDQIVDS
                     LQKNYDATVDFVADFRQETEVKTLNRKLKASGKLYFKRPGKMLWRYEEPKGQFVLADG
                     KNLYFYQPEQNQVIKSPLKNAFRSDIPLSFLLGLGNLRKEFSATLKGIEENQYVLRLE
                     PKGELGGFSEVLVGVGRSTSDIDWVSVRDAAANVTTIRFSAMRKGVGVQESLFRFQVP
                     DGVDIVELGQ"
     gene            6274..7629
                     /gene="rimO"
                     /locus_tag="VFN58_03940"
     CDS             6274..7629
                     /gene="rimO"
                     /locus_tag="VFN58_03940"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET8917175.1"
                     /translation="MSAGKQKFSIVSLGCARNLVDSEVMAGLLQQNHYDMVQEPEDAD
                     IVLVNTCGFIEAAKAESLDTIIEISRLKEEGKIKKLVVAGCLAQRYPQELAKELPEVD
                     LFIGTGEVPKIVEILQRQEADARIRQYVGIPQYLYDHTTPRVRATPSYTAFLKVSEGC
                     DHKCAFCIIPQLRGPHRSRSIDSVVNETALLSQGGVKEINLIAQDLTAYGRDRKDGTT
                     LLGLLNKMIEVPEVVWIRLLYAYPNFLDEPLLKVIRESDKICNYIDIPFQHISPRLLQ
                     RMRRGKSGSAVRETVEKLRDAIPGLTLRTSLIVGFPGETAKDFEALLDFVEETQFDRL
                     GVFKYSEEEGTTAADMDGKVSEDEKERRWQEVMDLQATISQKKNRALIGTIDRVIIEN
                     TDSEFGKLAGRTQAHAPEVDGVVFIDGLEAHSRPKPGDIVDIKITGALEYDLMGEILH
                     A"
     gene            7622..8083
                     /locus_tag="VFN58_03945"
     CDS             7622..8083
                     /locus_tag="VFN58_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET8917176.1"
                     /translation="MPNKTDPEKEKKQRREFLKQAADTLQETKKQLLKEMQGRVKEET
                     EGVKDEGRDTYDLASDERDREINFILNDREREKLLAIDEALQRIKDKTYGICESCEGE
                     IQLGRLKVLPFTRLCVKCQEETEKESKRQKTLEDERGYRKLVITDFEEDGF"
     gene            complement(8329..8706)
                     /locus_tag="VFN58_03950"
     CDS             complement(8329..8706)
                     /locus_tag="VFN58_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917177.1"
                     /translation="MNIKALFVGGLAVLLAGALASPMLAQEEKKAEKAEKAEKSEKAK
                     AQKVSGDIGLLDTEKNYMIVVTKDGKLITLDFTQKTKVTELKESQVKMGDIGLGSSAV
                     VEYTVKGEKNVLSKIEFTPAKGE"
     gene            complement(8761..9129)
                     /locus_tag="VFN58_03955"
     CDS             complement(8761..9129)
                     /locus_tag="VFN58_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917178.1"
                     /translation="MAAKPAPPAPVAKAEKAPPAEKAPAEKAEKATGEKAPEKAKATK
                     VAGDVGLLDLEKNYMILVTKEGKLVTVDFDAKTNATQIDPTPAKIADVGLGSSAVVVY
                     EQAGDKSVATSLEFKAAKGE"
     gene            9447..10151
                     /locus_tag="VFN58_03960"
     CDS             9447..10151
                     /locus_tag="VFN58_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8917179.1"
                     /translation="MVPTKVVIADDHTLFREGIKRMLSLEKDILVVGEASRGDEVTKL
                     VERTKPDVLLLDLKMPKGDIVQTLLEVKDINPATKVLVLTAFSEDENILNAAKGGARG
                     YVLKGIDFPTLLRAIKTIQGGGLWIDKELAAADVFEEIAQDQLDDRGDAREDNEAIKI
                     LTRRELEILKLVAEGLTNEEIGKKIFISEKTVKTHLTNIFDKLKVNNRFKAALLIMGR
                     TGSLEASSNVTRGRGQ"
     gene            10174..>10520
                     /locus_tag="VFN58_03965"
     CDS             10174..>10520
                     /locus_tag="VFN58_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917180.1"
                     /translation="MPSTLEETRQKRAMTPEDQWLLKKEQVLAWLRAGFALLGLIVIQ
                     FNPSRIARYPILSHVFLIAFLLYSLIIVYLARRERTDPKQIGLVTTCLDLMWVSSIVF
                     TTGAVQTPFFAYY"
BASE COUNT         2648 a         2673 c         2700 g         2499 t
ORIGIN      
        1 tggatgtgat ctcccgcgag ccgccgccag cagatcaccc gatcattcgg gcagcgaagg
       61 aactcgacaa tctgctcgta accccgcaca cggcctggag cgcgcgcgaa gcacgcgagc
      121 gattgcttgg agaagtcgaa gggaatattc gtgctttttt tgagggaaag ccgagaaata
      181 ttgtcgtcta gagaatcgga gaagcgagaa gcctaccggt tgggttcatt ctaaaatgga
      241 atatcgtcgt ctgccggcgg tgggccgccc atggccatct cgtcggatgc tccggactcg
      301 gtttcgggag caatccgcgt tccacctcca ccacccaaga atctgacccg ttgagcgacg
      361 atttcggtca tatatctttt gttgccgctt ttatcatcgt agctgcgggt tctaatactg
      421 ccttcgacaa agacttgcct gcctttggcc aggtactgcc cgcacgtttc accctgcttt
      481 ccccagacga ttatattgtg ccattcggtt ctctcttgcc gattgccctc cgcgtccgtc
      541 cacacttcag atgtcgctat gggaaagcgc gccacggcac gtccggtgga ggtgaaacgt
      601 acctcaggat ctttccccaa attaccgatc aagatcgctt tgtttaccga catcagagcc
      661 tcccgcctgt cacaaatcga gtttcgacgg aaacagcaaa attaccttat cgctgttata
      721 attttcagtc aataacgaag ttctctgcaa gttccgagaa cttcctcgag actgattcaa
      781 agattcgctc acaaaacaga gtagcatcca ttgctttttg acgtgctgtg cattaagttt
      841 attcagttat ttcaatcatt tgttctaaca aagcactgcc tacatttcca tgctttacag
      901 cttcaagctg cttgtgatct cggtggtcac cgtgccggcg gctctcttga taatcctgct
      961 cggagtcttt gatcgctacg ggaaatatgt ctacggaatc acccgtctgt ggacttggat
     1021 gatcctcatg atcggcggcg tgggattaaa agttactggc ctgagccaca tcgatccaaa
     1081 acggcagtat attttcatgg tgaatcatca gagccacatc gacatcccgg ttctaattca
     1141 gagcctccct tcttttcagc tgcgctggat agcgaaaagg gagttgcttt gggttccctt
     1201 tttcggttgg gcgatgtggg ctgcaaagca tattactgtg gatcgctcag atcgaatgga
     1261 tgccttaggc agcttaaaga aggccaagga gcgaatgaaa ggcggcattt ccttagtaat
     1321 tttccccgaa gggacccgta gcagcgacgg caatctgctg ccattcaaaa ggggcggcct
     1381 tttgctcgcc gtcaggacac aaacaccgat tgtcccggtt acaatcagcg gcagctgggc
     1441 gattctacca aaaggcgatt ggcgcattcg gggcggccaa atcgaagtaa ccgtcggagc
     1501 ccctgtatcc gtgaaaaatc atcggccggg aactttgcgg gagctctcgg tgcacgttca
     1561 agagctcata gaaaataacc tccaaaccgc agctcaaatc aagacgtcca agtccggaga
     1621 ggaacaatct acgactgcgg ttagatcgtc catggagaaa cgaaccgttt gaggtaccga
     1681 tggagccgct gagaatcata cccttgggag gattaggaga gtttggtctc aacatgatgc
     1741 ttctggagta cggggatgcg gccatcgcca tcgactgcgg cctcatgttt cccggcgcgg
     1801 atttactggg aatcgatctg gttattcccg acgtgagtta tttgttggag aaaaaggata
     1861 gcctcaaagg catcgtgctt agtcatgcgc atgaagatca cgtcggcgcg cttccttaca
     1921 ttctgagtcg tctttctgtt ccgatatttg gtactcgtct caccctgggg ctgttaaata
     1981 acaagctccg ggaacatagc cttgacaaca ctgcggacat tcgcgagatc actgcaggca
     2041 agccatggga gatcgcaccg tttcaagtcg aaggcatccg tgtcactcac agcttgatgg
     2101 attgcttggc gttggcgatt gaaacccctg taggaacagt catccatacg ggcgacttca
     2161 agatcgataa cacgccaatg gaaggcgaaa tgttcgattt tcagcgcttc ggcgcgtacg
     2221 gtgaaaaagg ggtgctgcta ctcctttccg attctacgaa tgtcgagcgt agtggctata
     2281 ctccgtccga acgagaagta ggcaggaatc tcgaacagat atttcaggag agcagaggca
     2341 agattttagt ctcgactttt tcgtcaagta ttccgcggat tcaacaggta gtcgatattt
     2401 ccgaaagctg cgggcgcagg gtagtgttga gcgggcgcag catgatcaga aacagtcagg
     2461 ttgccgctga gctaggttat ctccggcttc cccgaagttt catgaccgaa aatgaaccct
     2521 ggcatgagtt gccggccgaa aggctgacct tcctaacgac ggggagtcaa ggtgaaccct
     2581 tgtccgtact tcaccgcgtt gcattggacg atcacaagtc tatcaaggtc gatccggggg
     2641 atacggtgat tctctcctca aagtttatcc cgggcaatga gaaaacgatc tcgaatctca
     2701 tcaaccacct ttatcgccgc ggcgctgaag ttcattacga gaaggtatcg gagatccacg
     2761 tgtcgggcca tgcgagccaa gaggagctaa aaacgatgct ccaactcact cgaccacgtt
     2821 acttcgtacc catccacggg gagtaccgtc atcttgtgaa gcaccgcaaa ctcgcccaag
     2881 atgtcggaat cccgcaagac cattgtttcg ttttggaaga tggtgacgtt ttggagatca
     2941 gcgccaccag cgcaaaaaaa gtccagccca tccaagctgg tagagtgttt gtcgatggca
     3001 aaggcgtcgg ggatgttgga gatgtagtga ttcgggatcg gcgccattta tcggaggatg
     3061 gcatggtgct cgcggtcatg gcgattcacc agcaatccgg agagattgtt gccgggccgg
     3121 atctcatttc gcgcggcttt atgcgcgacg aagaggggga agaggtcctc ggacaggcca
     3181 gaaaagtcgt gctcgacatc ttggcgagcc tgaaccggga gacgcgtaca gatcccgcag
     3241 agctgcagga ggaggtccgc aaagcgctgc ggcgctactt ccggaagcga tttgagcgac
     3301 atcctgtcgt tctaccctac atcatcgaga cctagagact tttcgattca attctagcgc
     3361 gctatggaaa aaggtcgact caatcgtgaa atttggggtg tggtgatctg cttagccgcg
     3421 cttttgatca tcataagtct ctttacttac cttcccagtg atcgttcgtt taacacccct
     3481 tccggagcca tcgacacgaa caattggggc ggttttgtcg gcgcttatct ggcggacctt
     3541 ttgctccaag gactcggaat ggccgcatac cttttgccga tattcctttg cctcattgcc
     3601 cttcaaatgt tccgcgcaaa ctacacccga attccgtaca gtaaggtcat cggctacgct
     3661 gttcttctca tcagcacagc ggtcattcta tgcctcttca tcgagtctga gaatgcccga
     3721 gaagccggcg gaatcgtggg tgggtttcta aaggagaccg tgctcattcc tctgtttgga
     3781 cgcatcagcg ccgtattgat cagcctcttt gcgctcatcc tctccctcat gcttctgact
     3841 cagaactcgc ttttggacat tctaaatagc gccaagaaac agttgagtgc gctcaagaag
     3901 ttgttagtcc ccgccgtcct caaccgtctt cgggaactga aagagaaaaa ggaaaaactc
     3961 aagggggaaa acaccaaaaa ggagcggcgc gactatgtgc caccacccat cgttctgaaa
     4021 gaagaccaca aggaggaccc ggctagaaag atccagaaga aaactgctat ttcgccggag
     4081 caattcaagc tgccggagat cggtgaaggc tataagttgc ccccgcccga gttattggac
     4141 cttccggaag aagtcaatct aaaactagat acggaaacac tgcacgccaa ttcgctcatt
     4201 ctgcagaaaa agctcgctga tttcgatgtg gaaggcgaag tggttgccgt gcgtcccgga
     4261 cccgttatca ccatgtacga gttcaagccg gcaccggggg ttaaggtccg ccgaatcgtg
     4321 acgcttgcgg atgacttagc catggcctta cgggctgtaa gtgtacgcat cctagccccg
     4381 atcccaggag agtccgtcgt gggcatcgag attccaaatc cgcggcgcga gatcgtcttc
     4441 ttgagagagg tcattgaaag cgaagcgtat caacgcgcgg actccaaatt aacccttgcc
     4501 ttagggaagg atatcagcgg ctctcctttc gccaccgatc tggcgcggat gcctcactta
     4561 ctggtcgccg gggccacagg aaccggaaaa tcggtgtcca tcaacgcgat gattctcagc
     4621 atccttttca agtcgacccc gcaggatgtg caattcatta tggtcgaccc gaagatgctc
     4681 gaactcacga tctatgagga cattccccat ctgctggttc ctgtcgtcac ggaccccaag
     4741 aaggcggcgg cggcgctttt ctgggcgatg gatgaaatgg atcaccgcta tcgtctgatg
     4801 agagacaagg gggctcggaa tatcgataat tacaatcggg tgttggaaaa ggaaaccgca
     4861 actaagaaag gcgtcattga gctgatggaa ggggacccaa atcacgcgac atctgaattt
     4921 ggcggtgacc tggataaagc gccacccctc gtgcatgaac ggcttccgag gattgtgatc
     4981 atcatcgacg aactggccga tttgatgatg tcagtcggca gagatatcga agagtacatt
     5041 actcgactgg cacaaaaggc tcgggccgca ggtatccatc ttatccttgc gactcaacgg
     5101 ccatcggtcg atgttatcac cgggcttatc aaagccaact ttcctgctcg gatatccttc
     5161 caagtgacgt cgcgcgtcga ttctcgaacc atcctggatt ctatcgggtc ggaaaagctc
     5221 ttgggcaatg gcgacctcct gttcttacct ccgggaaccg cgcggctgac ccgcctccac
     5281 ggcgctttcg tctccgatca agaagtccgc aaggtgatga agtttatcaa acagcaggca
     5341 agccctcgtt accgccccga ggtctttgaa gtcaagaggg aggtcgaggc tgccgcagtc
     5401 gatgaagaat atgatgaaat gtacgaccag gcggttgccg ttgtaaccga aacccaacaa
     5461 gcgtcgatct ctatgattca gcggcgattg cgcgtaggat acaatcgtgc ggcccgaatg
     5521 atcgaacaaa tggaacggga aggcgtagtc gggccgccgg atggcgccaa accacgggaa
     5581 gtgtatgcta gaaagctcga agtttagaac ctccccaaag attttgtcgc ggttaatttt
     5641 cgctcttgcg gtcacaatca caagcgctga aagctccgcc acggagaacc gaagtggcga
     5701 tcagatcgtc gacagcctcc agaaaaatta cgacgccact gtcgacttcg ttgcggactt
     5761 tcgccaggaa accgaggtaa aaacgcttaa ccgtaaactc aaagcctcgg gcaagctata
     5821 tttcaagcgg ccgggaaaga tgctttggcg ctacgaagaa cctaaagggc agttcgtctt
     5881 ggcggacgga aaaaatcttt acttctacca gccggaacaa aaccaagtca tcaagagtcc
     5941 tctaaagaac gctttccgtt ctgatatacc gctgtctttc ttgctgggac taggcaatct
     6001 aagaaaagaa tttagtgcca cgctgaaagg catcgaggaa aaccagtacg tgctccgttt
     6061 ggaacctaag ggcgaactgg gcggattcag cgaggtactc gtaggagtcg gccggagcac
     6121 gtctgatatc gactgggtaa gcgtacgcga cgccgctgcg aatgtcacaa caatccgctt
     6181 ttccgctatg cgcaaagggg ttggagtcca agaatctctt ttccgtttcc aggtgcccga
     6241 cggtgttgat attgtggaat taggtcaatg acaatgagtg ccggcaaaca aaaattcagc
     6301 atcgtgagtc taggttgcgc ccggaatttg gttgattccg aagtcatggc gggtctattg
     6361 caacagaacc actacgacat ggtgcaagaa cctgaggacg cagatattgt gctggttaac
     6421 acttgcggct tcatcgaggc cgctaaagct gagtccctcg acacgatcat tgaaatttca
     6481 cggctcaaag aggaaggaaa gattaagaag ctcgtcgtcg ccggttgcct tgcgcaacga
     6541 tatccgcaag aactagccaa ggaattgccc gaggtagacc ttttcattgg aaccggtgaa
     6601 gttccgaaaa tcgtcgagat ccttcaaagg caagaggcgg atgccaggat tcggcaatat
     6661 gtcggcattc cacagtacct ctacgatcac accactcctc gcgtcagagc cactccttct
     6721 tacactgcct ttctcaaggt ctctgagggc tgcgatcaca aatgtgcctt ctgtattatt
     6781 ccacagctcc gggggccgca tcgcagtcgc tccattgact cggtggtcaa cgagaccgct
     6841 ttgttgtcac aaggaggggt caaagagatc aatcttatcg ctcaggattt aactgcctac
     6901 ggaagggatc ggaaagacgg cacgacgctc ttgggtctgc tcaacaaaat gattgaagtt
     6961 ccggaggtcg tgtggatccg gctcctgtac gcgtacccga actttttgga tgagcctctg
     7021 ctaaaagtaa ttcgcgaaag tgataagatc tgcaactata tcgatatccc ttttcagcac
     7081 attagtcctc ggctgctgca aagaatgaga cgaggaaaaa gcggcagcgc cgtacgggaa
     7141 acggtcgaga aactccgtga tgctataccg ggactgaccc ttcgaacgtc tctcatcgtc
     7201 ggatttcccg gtgaaaccgc caaagacttt gaggcgcttt tagactttgt tgaagagact
     7261 caattcgatc ggttaggcgt ctttaaatac tcagaagagg aaggcaccac tgcggccgac
     7321 atggatggca aagtttccga agacgagaaa gagaggcgtt ggcaagaagt aatggatctc
     7381 caggccacga tctcacaaaa aaagaatagg gccttgattg ggaccattga ccgcgttatt
     7441 attgaaaaca ccgattcgga attcggcaaa cttgccggac gaactcaagc ccacgctcca
     7501 gaagttgacg gtgttgtttt tatcgatggt ctagaggcac attcgcgacc taagccagga
     7561 gatatcgtcg atatcaagat taccggtgct ctagaatacg acctgatggg tgagatactc
     7621 catgcctaat aaaaccgacc ccgagaagga aaagaaacag cgaagagagt ttctgaagca
     7681 agctgcggac actttgcagg aaaccaagaa gcagctactc aaagaaatgc aaggccgcgt
     7741 caaggaagaa accgaaggcg tcaaagacga gggccgagac acgtacgatc tggccagcga
     7801 cgagagggat cgggaaatca actttattct caacgaccgt gaacgtgaaa agttgctcgc
     7861 cattgatgaa gcgttgcagc gaatcaaaga caagacgtac ggcatctgtg aaagctgcga
     7921 gggtgaaata cagctaggcc gtctgaaagt tctgcccttc acgcggctct gtgtaaagtg
     7981 ccaggaagaa actgaaaaag agagcaagcg tcaaaagacg cttgaagacg agcgcggcta
     8041 tcgcaaactt gtcattactg acttcgaaga agacggcttc taatcgtgcg ctgtccagta
     8101 ggagatagct gcccgccttt agagtttccg gtcgataaat ccacgcccaa actcgttatc
     8161 tgtgatcgac tttatttctg agcgatatgc gttttagtcc tgttttgccg aagaatccag
     8221 gcgttgatga aaatctgaaa tggaattgga gaggctgtaa taaaaaaaag gagtgggtta
     8281 tcgcctaacc cactcctgca tatcgagata attctaacct gccgaagatt attcgccctt
     8341 tgcaggggta aactcgattt tgctcagaac gtttttctct ccctttacgg tgtactcaac
     8401 cacggctgaa gagccaagac cgatgtctcc cattttaact tgactttctt ttaactccgt
     8461 taccttggtt ttttgggtga agtcgagtgt gatcagcttg ccgtccttgg ttaccacaat
     8521 catgtaattt ttttcggtgt cgagcaagcc aatgtctccc gagaccttct gtgctttcgc
     8581 cttctcggac ttctccgctt tttccgcttt ctccgccttc ttctcttctt gggcaagcat
     8641 aggcgacgca agcgcccccg ctagtaatac cgctaatcca cccacgaaca gtgctttgat
     8701 attcattcgt tcattcctcc tgattcagaa atcgtaaaat gtcagaccta aacctcgcaa
     8761 tcactcaccc tttgccgctt tgaattctag gctagtcgcg acgctcttat ctccggcttg
     8821 ttcgtacacg accaccgccg aagaccctag tccgacatca gctatcttgg ccggagtggg
     8881 atcgatctgg gttgcgttag tcttcgcatc aaagtccacg gttacgagtt tcccttcctt
     8941 agttacaagg atcatgtagt tcttttcgag atcgagcagt ccaacatcac cggccacctt
     9001 agtcgccttc gctttttccg gtgctttttc ccctgtcgct ttttcagcct tctccgcagg
     9061 ggctttttca gccgggggag ctttctcagc cttcgctact ggagctggtg gggctggctt
     9121 tgcggccata tcatcctgag tagaacagga agagagcgca aacaacatgt aaacaaatat
     9181 taccgcgctt gctgaaaaca aacttttcat cttacctccc taacactgca aatttgtact
     9241 gagtttcaaa ttaattatcc tgacagcctc ctcatctcaa tactctttgg gtagtatttt
     9301 ttcggctaaa actagcacga taatgccttt atataagacc agaggcttag gtagtctgcg
     9361 ttaacaggcc aaaatctcta ttggcagctc taatcttttg aggtatttta agtcagttgc
     9421 ctgcccacag gcgaaggaga acaaaaatgg tgccgaccaa agtcgtaatt gccgacgacc
     9481 acacgctgtt tcgcgaagga ataaaaagaa tgttgtctct tgaaaaggac attctcgtcg
     9541 taggggaagc ctccagagga gacgaagtaa caaagcttgt ggaacgcacc aagccggatg
     9601 ttttactcct cgatctcaaa atgcccaagg gcgacattgt ccagactctg ctcgaagtga
     9661 aggacataaa tccggcgacc aaagtactcg tgctcactgc attctccgag gacgaaaata
     9721 tcttaaacgc ggccaaggga ggggcccgtg gctacgttct caagggcatt gacttcccga
     9781 cgttgctacg agccatcaag accatccagg gcggcggttt gtggatcgac aaagagctgg
     9841 ccgcagccga cgtatttgag gaaattgccc aagaccagct tgatgatcgg ggcgatgcca
     9901 gagaggacaa tgaggccatc aagattctaa cgaggagaga actggaaatt ttgaaattgg
     9961 tagcagaggg gctgacaaac gaggagatcg gcaaaaagat attcatcagt gaaaaaaccg
    10021 taaagaccca tctgacgaat attttcgaca aactcaaagt caacaatcgg tttaaggcag
    10081 cactcctaat catgggtcgc accggaagtc tagaagcttc ctctaacgtc acgagaggaa
    10141 gaggtcagta ggagttaagg gggagttgga tccatgccct ccacattaga agaaacccgc
    10201 cagaaacggg cgatgactcc cgaggaccag tggctgctaa aaaaggaaca agttctagca
    10261 tggctaagag caggatttgc tctccttggt ctcatagtga ttcaattcaa tccctcgagg
    10321 attgcaaggt accccatact ttcccatgtt ttcctcatcg cttttttgct ttacagccta
    10381 atcattgtgt atctggctcg gcgagaaagg acggatccaa agcaaatcgg gctggtcacg
    10441 acttgccttg acctaatgtg ggtttcttct atcgtcttta cgaccggtgc agtccaaact
    10501 cctttttttg cttactattt
//
LOCUS       DASSWZ010000161         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_698565, whole genome shotgun sequence.
ACCESSION   DASSWZ010000161 DASSWZ010000000
VERSION     DASSWZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698565"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFN58_03970"
     CDS             complement(<1..584)
                     /locus_tag="VFN58_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917181.1"
                     /translation="MKSIKLLIVLVLLSFTSKAVGQDPRLIDAAKKEGGKVIIYGSPE
                     TPVVDAVIQAFQKKTGLSADYWRASAMSVMNRAMSEYRAGNALYDVVLNNTDPLYIMA
                     KEGMVAKYDSPTAKKYPNDQIDPYLGPISRLGIVGIVYNRNLVRPENAPRTLEDLVQP
                     KYRGMLVMADPTLHVTTIQWLSSLHKIMGKEKAE"
     gene            736..1803
                     /locus_tag="VFN58_03975"
     CDS             736..1803
                     /locus_tag="VFN58_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917182.1"
                     /translation="MQIIDADGHVNDRPCMDEISKYMPPGNRTQVFPAFDHIHFHFLE
                     GGERRSRTGTVGPKEWTEFMDDTGIDWTVVYPTAGLAVGRIISEDWAVAACRAYNNWL
                     YEKFTHVSSRIKGMALIPIQDPQAAVNELHRAIKELGMVGAMLPSNGEGIKGHLGAKI
                     YWPIYEEAEKLGCPLAVHVGCLHHLGMDTIGVYYPVHALGHPFGIMAQAAGMLSYGVF
                     DRFPKLRVGFLEGGATWVPFFMDRLDRSYPHHLQVDVDGEFLPSSKPDVTASEYFRRH
                     VKAGRIFVGFDCDDRGLGFAVKEAGNEPFLFATDFPHESFSAESCRREINELLGREDL
                     SPTDKEAILAINAKRFYGIDI"
     gene            complement(1840..2394)
                     /locus_tag="VFN58_03980"
     CDS             complement(1840..2394)
                     /locus_tag="VFN58_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET8917183.1"
                     /translation="MADKNHLILTAVGPDQVGLVEKISEFIARHSSNIEDSKMAVFCG
                     EFALIILISGEGGNLHKIANHYRELEIETGLAIAIKTPTSRKPAESFLPYTLTASCMD
                     HPGIVYQLSGILSSLGINIESMETKTYAAPVSGTPIFRLEANISIPTRININSLRERF
                     AAIQKEENIDIELSLVHGGATKTA"
     gene            complement(2415..3701)
                     /locus_tag="VFN58_03985"
     CDS             complement(2415..3701)
                     /locus_tag="VFN58_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917184.1"
                     /translation="MSASWEPASIISREEVLKLHDEVAARPSFPTRIYEDVFRIQALE
                     MNWDIGVAIYQPQDSVNNPTGPDGKKVGVFLLHGGVSDYKSVDRVARALAEKFGIKVA
                     SMTFPGRFYLLDPSRDWPGDVENHDGTARTPLWTRETHITKDQYEIVQDTSKRESYGT
                     LISLSAKQGTEFYDRMAAWPVAFEEAMKETAQRHLPEGQFSIYIHGHSTGGPFAMMAT
                     QRIPNISGIVGYGTSPFGYMYTEVTGDRWQFPFNWLRLRTWRDTARYLYEGMKDKGIG
                     LPMLMELTLERWETGKKRPNFKAEDFIHKNSTNSLAAAARVSAARLKMSNRETDALVQ
                     KYLGYTRELSGTGMKPVPPFLSLHGVNDDTVTLARCERSLSLFAKLNPAPKVRCVSLG
                     AGVHTWSYTETELPQGVGPAVAKIWYDAIMNGFYMV"
BASE COUNT          828 a          982 c         1120 g          989 t
ORIGIN      
        1 ttttccgctt tctcctttcc catgatcttg tgcaggctgc tcagccactg gatggtcgtg
       61 acatgaagcg tcggatccgc catcacgagc atgccacggt attttggttg caccagatct
      121 tcgagtgttc tgggcgcatt ctcgggccga actaggttcc ggttgtaaac gatgccgacg
      181 ataccgagcc ggctgatcgg gcctaggtat gggtcaatct gatcgtttgg gtatttcttt
      241 gcggtcggcg aatcgtattt ggccaccatg ccttctttgg ccatgatgta aagtggatct
      301 gtgttgttca gcaccacgtc gtaaagcgcg tttcctgcgc gatactcgct catggctcga
      361 ttcatcacgc tcatagcgga agctcgccaa tagtccgcgc tgagtccggt tttcttttga
      421 aaagcttgga tcaccgcgtc cacgacaggt gtttccggcg agccgtagat gatcaccttg
      481 ccgccttcct ttttcgccgc atcgatgagt cttgggtcct ggccgacggc tttggaggta
      541 aaggatagaa ggacgagaac tatcaacagt tttatagact tcattttagc ctcgctcttt
      601 ggcttcagag gttcaatcta tctatgcgac cttctcctaa cacaccaggg tttttagggt
      661 caattcccat gcttgacagg cgcggaaagc gcttgttata gcgctgtcag aaactgctcg
      721 aaaggaagaa agatcatgca gatcatcgat gctgatggac acgtgaacga ccggccttgt
      781 atggacgaaa tctcaaaata catgccaccg gggaatcgca cgcaagtgtt tcccgctttc
      841 gaccacattc actttcactt tctcgaaggg ggcgagcgtc gatcgagaac gggaaccgtt
      901 gggccgaaag agtggacgga atttatggac gatacgggaa ttgattggac agtcgtctat
      961 ccgactgcgg ggctggctgt cggccgcatc atctccgaag actgggcggt ggctgcctgc
     1021 cgggcgtaca acaactggct gtacgaaaaa tttacccatg tgagctcgag aatcaaagga
     1081 atggcgttga ttcctattca agatccgcaa gcagcggtca atgagctgca ccgcgcgatc
     1141 aaagagctcg gtatggtcgg cgcgatgctg ccatctaacg gcgaaggtat taaaggtcac
     1201 ttgggagcga agatctattg gcccatttat gaagaggcgg aaaagctcgg gtgtccgttg
     1261 gcggtgcacg tgggctgcct gcatcatttg ggtatggata ccatcggcgt ttactatcct
     1321 gtccacgcct tgggtcatcc gttcggcatt atggcgcagg cggccggtat gttgtcttac
     1381 ggtgttttcg atcgctttcc caagctccgt gtcggttttc tcgaaggcgg ggcgacatgg
     1441 gtcccgttct tcatggaccg gttggatcgt tcctatccgc accacttgca agtggatgtc
     1501 gatggagaat ttttgccgag ttcgaagccc gacgtgaccg ccagcgaata ttttagaaga
     1561 catgtcaaag ccggccgaat ttttgtcggt ttcgactgcg acgacagagg ccttggcttc
     1621 gccgtgaaag aagcgggcaa cgagccgttt ctgtttgcca ccgacttccc gcatgaaagc
     1681 tttagcgccg agtcctgtag gagggaaatc aatgagctgc tggggcgcga ggacttatca
     1741 ccaaccgaca aggaagcgat cttggcgatc aacgccaagc ggttttacgg aatcgatatt
     1801 tgatccggca gatgttgtgt aagaggaaag caactttcct taagccgtct tcgtcgcgcc
     1861 tccgtgcact aatgaaagct caatgtcgat gttttcttcc ttttgaatcg ccgcgaagcg
     1921 ttcccgcaga gaattgatgt tgatcctggt gggaatggaa atattggcct ccaagcgaaa
     1981 gatcggtgtg ccgctcaccg gcgcagcgta agtctttgtt tccatggatt cgatgttgat
     2041 gccgaggctg ctcaaaatgc cgcttagctg atagacgatg ccgggatggt ccatacagga
     2101 cgctgtaagt gtgtagggca gaaatgattc cgccggcttt cgggatgtgg gcgtcttgat
     2161 tgcaatggcc aagccggtct caatttccag ctcgcggtag tggttggcga tcttgtggag
     2221 gttaccgccc tcgccgctga tcaatatgat cagtgcgaac tccccgcaga agacagccat
     2281 cttgctgtcc tcgatgttgg agctgtggcg ggcaataaat tcggaaattt tttccaccag
     2341 tccgacctga tccggtccga cggcggttag gatgagatga ttcttatctg ccatgggtat
     2401 tctcccggta acgtttatac catgtagaat ccgttcataa tggcatcgta ccagatcttg
     2461 gccactgcgg ggccgacgcc ttgcggcagc tccgtttcgg tgtaggacca ggtgtgtacc
     2521 cccgcgccga gggaaacgca gcgcactttg ggtgcgggat taagcttagc gaataacgaa
     2581 agagatcgct cacaacgggc aagggtgaca gtgtcgtcgt tgacgccgtg aagcgacaaa
     2641 aagggcggta ccggcttcat gccggtacct gaaagctccc gtgtgtagcc gaggtatttc
     2701 tgaaccagcg cgtcggtttc tcgattagac atcttcaagc gcgcggcgga aactcgcgcc
     2761 gcagctgcca atgaattcgt tgagtttttg tggataaagt cttcagcttt gaagttgggg
     2821 cgcttttttc cggtttccca gcgttctaac gttagttcca tgagcatggg aagcccgatc
     2881 cctttatctt tcattccttc gtaaagatag cgggccgtgt cgcgccaggt gcggagccgc
     2941 agccagttga agggaaattg ccacctgtca cccgtgactt ccgtatacat ataaccgaac
     3001 ggtgaagtcc cgtagccgac gatcccggag atattgggga ttcgttgcgt cgccatcatg
     3061 gcgaacggcc ccccggtgga gtggccatga atgtagatcg agaactggcc ttccggcaag
     3121 tgacgctgtg cggtttcttt cattgcctct tcaaaggcca cgggccaggc tgccatgcgg
     3181 tcatagaatt ccgttccttg tttcgctgac agagatatga gggtgccata gctttcgcgc
     3241 ttcgaagtat cttggacgat ctcatattgg tcctttgtaa tgtgggtttc cctggtccag
     3301 aggggagtcc gtgcagttcc gtcatggttc tcgacgtcac cgggccaatc tctgctgggg
     3361 tcgagtaaat aaaaccttcc ggggaaagtc atactggcga ctttgatgcc aaatttttcc
     3421 gccagcgcgc gcgcaacgcg gtcaacagat ttgtaatcgg aaacgccgcc gtgaaggaga
     3481 aaaacgccga cttttttgcc gtcaggtccc gtcgggttat tgactgaatc ttgcggttga
     3541 tagatcgcga cgccgatatc ccagttcatt tccagagctt gaatgcgaaa gacgtcttca
     3601 tagattcgtg tcgggaagct gggtctcgcg gcgacttcat catgaagttt gagaacttct
     3661 tctcgagaga tgatagaggc tggttcccag gaggctgaca tggctaaata tcctccggca
     3721 tgctgggccg acatttttta gactacgcga cgggaactgc gtatgtcaac gtggatcgga
     3781 aaaagcttcg tataatacgc ggccggtcgc gtctttagga atgggtacgc tcaaaagttc
     3841 ggcgagggta ggtgcgaaat cgaggattcg cacgccgcgc aactggtgtc ctacttggac
     3901 ccctggcccg ttcagtacc
//
LOCUS       DASSWZ010000162         4829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_373221, whole genome shotgun sequence.
ACCESSION   DASSWZ010000162 DASSWZ010000000
VERSION     DASSWZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373221"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFN58_03990"
     CDS             complement(<1..452)
                     /locus_tag="VFN58_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917185.1"
                     /translation="MKNSLVWPVFAGLLIAITSLFVRAEDPLTRPPEETRETHLKNLK
                     QLTFGGQNAEAYFSFDGTKLIFQSTRPPFECDQIFSMNVDGTDVKLLNSGKGRTTCGF
                     FFPDGKRFIYASTHPAGDACPPAPDRSQGYVWPIYPAYEIFSASPNGSG"
     gene            908..2827
                     /locus_tag="VFN58_03995"
     CDS             908..2827
                     /locus_tag="VFN58_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HET8917186.1"
                     /translation="MIKGTYEEVYGRWQNAPESFWAEVAEGIHWYKKWDRVLDASRPP
                     FYRWFTGGTVNTCYNALDRHVEQGRGNQPALIYDSPVTRTIKTFSYRELLDEVARFAG
                     VLVCQGIQKGDRVIIYMPMVPEAVIAMLACARIGAIHSVVFGGFAAHELASRINDARP
                     KLIISASCGIEVDRVVPYKPLVDRGIEIASVKPERCIILQRPQATASMIAGRDLDWSE
                     LMADSKPVDCVPVAATDPLYILYTSGTTGLPKGIVRDNGGHLVALKWSMKNIYGVEPG
                     EIFWTASDVGWVVGHSYIVYAPLLNGNTTILYEGKPVGTPDPGAFWRLISEHNVRVLF
                     TAPTAFRAIKREDPTGDYMHKYDLSCFRTLFLAGERCDPDTLLWAEKQLGVPVIDHWW
                     QTETGWPIGANCVGLGKLAVKPGSCTKPVPGYDVRALSDDGQELSGGQIGNIVIKLPL
                     PPGCLPTLWNNDEGFQKAYLSRYSGYYLTADAGYKDEDGYLWIMSRTDDIINVAGHRL
                     STGAMEEVLAAHPDVAECAVVGIADTIKGEIPLGLIVLKAGVRRPHGEIVTEVVEMVR
                     ERIGPVASFKVAMIVKRLPKTRSGKILRGTIKNIADGVDYRLPPTIDDPAILDEITVD
                     LAALGYPGNLSKKPA"
     gene            2965..3906
                     /locus_tag="VFN58_04000"
     CDS             2965..3906
                     /locus_tag="VFN58_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET8917187.1"
                     /translation="MTLSTTSRPEAWPSLALEAWSGTCATLHMWTQIVGKIRLAQSPW
                     VNHSWHVTLYVTARGLTTSPIPYDTRTFQIDFDFISHELTIESSDGGVGTVPLQPQSV
                     AMFYKRLTGELERIGLNVQINKKPNEIADAIRFDRDESHAAYDPDYANRFWRILIQAD
                     RIFKQFRACFIGKCSPVHFFWGAPDLAVTRFSGRRAPEHPGGVPNLPDRVAREAYSHE
                     VSSCGFWPGGGLILYPAFYSYAYPEPDGFAKAPVKPNGAFYSTDLHEFILPYDAVRQS
                     ESPDQTLLDFLQTTYEAAANLAGWDRNSLERREDPSR"
     gene            3932..4339
                     /locus_tag="VFN58_04005"
     CDS             3932..4339
                     /locus_tag="VFN58_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET8917188.1"
                     /translation="MRPTKSKSWFTRFAKSTARWSGRPITFVLAVLVIVVWAITGPIF
                     GFSDTWQLIINTGTTIITFLMVFLIQSTQNRDSEAMQVKLDELIHVMKDAQNALLDLE
                     ELEEDELDRIRAGYQRIAEQARGELRKSATEKR"
     gene            4399..>4829
                     /locus_tag="VFN58_04010"
     CDS             4399..>4829
                     /locus_tag="VFN58_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET8917189.1"
                     /translation="MADRQRQIIAIGGGGFYRDSENLGLERYIVQQTGTADPRVAFVP
                     TASAEPDNYLVSFYTAFLKLGCRPSHLSFFKRTADLRAYLLNQDVIFVGGGNTKTMLA
                     VWREWGVPEILREAWESGILLTGVSAGAICWFEQGVTDSWAG"
BASE COUNT         1112 a         1233 c         1382 g         1102 t
ORIGIN      
        1 ccggaaccgt tgggactagc actgaagatt tcatacgcgg gataaatggg ccaaacatac
       61 ccttggcttc ggtccggcgc cggcgggcag gcatcgcccg ccggatgagt ggaggcataa
      121 atgaatcgct tcccatccgg aaagaaaaac ccacaagtcg tgcggccttt cccgctgttt
      181 aggagtttca catcggtgcc gtcgacattc atgctgaaga tctgatcaca ctcgaacgga
      241 ggccgggtgg attggaagat caattttgtg ccatcgaagg aaaaatacgc ttcggcattc
      301 tgcccaccaa aggtgagttg cttcaagttt tttagatgcg tctcccgcgt ttcttcaggt
      361 ggacgtgtca gcgggtcttc ggctctgacg aaaagggaag tgatggcaat cagcaacccc
      421 gcgaagacag gccatacgag tgaatttttc attgctggat aaaagttaat aaggcggacg
      481 gttgcttcac tctacttaaa aaatccgcat atgtcacact aaagtaacac gattgtagag
      541 ggagcccttg ctggcggaca cttgcatcga gaaatcctaa tcagcggctg ccaatttcaa
      601 tttgagttaa gcctgacagc ggagtaaaga acaatgagaa tcgacgtcgc tgtttgacgc
      661 taagtttccg caaataatgt agagcattga gtcatggcag tcagcgtatt tgtatcgtat
      721 agccacaacg atgttgcgct cgtcacgccg atagtgaagc tcctgcgccc aataaggcgc
      781 tcgtatttcg atgtaacaat cagcgtcgcg ctgtctgccg gcgtgtggcc aacgccgatc
      841 cgatttcaaa gggctggcga ttgagcaatc ggttacatag gtaactagga ggaaggagga
      901 cgatccgatg atcaagggga catacgaaga agtttatggc cgatggcaaa acgcgccgga
      961 gagtttctgg gccgaagtag ccgaagggat tcactggtac aagaaatggg acagggttct
     1021 tgatgcttcg cgcccgccgt tctaccgctg gttcaccgga ggaaccgtga acacttgcta
     1081 caacgccctt gatcggcacg tagaacaagg ccgcggaaac cagccagcgc ttatctacga
     1141 tagccccgtc actagaacga tcaagacctt cagctatcgc gagttgttgg atgaagtggc
     1201 gcgttttgcc ggagtgctgg tttgccaggg gattcagaaa ggggaccggg tcatcatcta
     1261 tatgccgatg gtgccagagg ccgtcattgc catgctggcc tgtgcccgga ttggggcgat
     1321 tcactcggtg gtcttcggag gatttgccgc ccatgagttg gccagtcgca tcaatgacgc
     1381 gcgacctaag ctgatcattt cggcttcctg tggcatcgaa gtggaccggg tggttcctta
     1441 taaacccctg gtcgataggg gaattgagat cgcatcagtg aagcccgagc ggtgcattat
     1501 tctccagcgt cctcaggcga cagcgtcgat gatcgcgggc cgcgatctcg actggtcgga
     1561 actaatggcg gactctaagc cagtcgattg tgttccagtt gctgctactg atccgctcta
     1621 catcctgtac acctccggta ccacgggtct tcccaaagga atcgtccgcg ataatggcgg
     1681 ccatctggtt gcgctcaagt ggagcatgaa gaatatctat ggggtcgaac cgggtgagat
     1741 cttctggacg gcctctgatg tgggttgggt agtgggtcac tcctatatcg tctacgcccc
     1801 tctcctcaac ggcaacacga cgattctcta tgagggcaaa ccggtcggca cccccgaccc
     1861 cggcgctttc tggcgcttga tctctgagca taatgtgaga gtcttgttta cggcaccaac
     1921 ggccttccga gccatcaaac gggaagatcc taccggcgat tacatgcata agtatgattt
     1981 gagttgtttc cgcacccttt ttcttgctgg cgagcgatgc gaccccgata cgctcctctg
     2041 ggccgagaag cagctcggtg ttccggtcat tgatcactgg tggcaaaccg aaaccggatg
     2101 gccaatcgga gccaattgcg ttggcttggg taagcttgcg gtgaaacctg gatcatgcac
     2161 aaaacccgtt ccggggtatg acgtacgcgc actgagcgat gacggccagg agctatcggg
     2221 aggtcaaatc ggcaacatcg ttattaagct tccgctgccg cccggttgtc ttcccactct
     2281 gtggaacaac gacgaaggtt ttcagaaggc atatctgagc cgttattccg ggtactactt
     2341 gactgcggat gcaggttaca aagatgagga tgggtacctc tggattatga gccgtacgga
     2401 cgacatcatc aatgtcgccg gccatcgttt gtccaccggt gcgatggaag aggtgttggc
     2461 agcgcatccc gacgttgccg aatgtgctgt tgtgggaata gccgacacaa tcaagggaga
     2521 aattccgttg ggtctgatcg tgcttaaagc gggggtgcgt cgtcctcacg gcgagatcgt
     2581 tacggaagta gtcgagatgg tacgcgagcg gatcggcccg gtggcgtcct tcaaagtcgc
     2641 gatgatcgtt aagcggctgc ccaagactcg atcgggcaag attttgcgcg gtaccatcaa
     2701 gaacattgcc gacggagtcg actaccggct cccgccgacc atcgacgatc cggcgattct
     2761 cgatgaaatt acggtagact tggcggcgtt aggctatcca ggcaacttga gcaagaagcc
     2821 ggcctaactc ctccggccga cggcacgaaa agatgagata tgctcaccag tgtaattcaa
     2881 ttatgaatcg caggtctgac ggtgtcttga ctgttcaatc agcggcggaa ttttttgttc
     2941 actcactaaa gaaaggagga ttttgtgacc ctatcgacta cttcgcgtcc ggaggcatgg
     3001 ccaagcttgg ctctggaggc ttggagcggc acttgtgcca cgttgcatat gtggacccag
     3061 attgttggga aaattcgcct cgctcaaagc ccgtgggtta atcactcgtg gcacgtgacg
     3121 ctttacgtca cggccagggg gcttaccact tcgcccattc cttatgacac gagaaccttc
     3181 cagatcgact tcgacttcat cagtcatgag ctgactatcg agtccagcga cggcggggtt
     3241 ggtacagttc cgcttcagcc tcaatcagtg gcgatgtttt acaaacgcct gacgggagag
     3301 ctagagagaa ttgggctgaa cgtacagatc aacaaaaaac ctaatgagat tgccgatgcc
     3361 attcggttcg accgtgacga atctcacgca gcatacgatc ctgactacgc gaaccggttc
     3421 tggcgaatct tgatccaggc ggatcgcatc ttcaaacaat ttcgcgcgtg cttcatcggc
     3481 aagtgcagcc cggtgcattt tttctggggc gctcccgatc tggcggtcac acgtttctcc
     3541 gggcgaagag ctcccgagca tcctggcggc gttcccaacc taccggaccg agtggcgcgt
     3601 gaggcatact cgcacgaagt cagtagctgc gggttttggc ccggcggtgg cctgattctc
     3661 tatcccgctt tctattccta tgcctatccc gagcctgatg gcttcgcgaa agcacccgtc
     3721 aaaccgaacg gggcgtttta ctctacggat ctccacgaat tcatccttcc ctacgacgct
     3781 gtccgccagt cggaatctcc cgatcagacg cttcttgact tcctacagac gacgtatgag
     3841 gcggcagcga atttggctgg gtgggatcgc aattcgttgg aacgtcgtga agatccgagc
     3901 cgctgatctt cgaaatcacg ggagctagag catgagaccg acaaaatcaa aatcgtggtt
     3961 tacgcggttt gccaaatcca cggcgcgttg gagcggcaga cctatcactt ttgtgctggc
     4021 cgtgcttgtc atcgtcgtat gggcaatcac ggggccaatc ttcggcttca gcgatacatg
     4081 gcaactgata ataaacacag gcacaaccat catcacgttt ttgatggtgt tccttatcca
     4141 gagcacacag aaccgggact ccgaagcgat gcaggtaaaa ttagatgaat taattcatgt
     4201 aatgaaagac gcacaaaacg cccttttaga cttggaagag cttgaagagg acgaactcga
     4261 tcgcatccgc gccggatatc aaagaatcgc agaacaggcc agaggggagc ttcgtaaaag
     4321 cgccacagaa aaaaggtaat ggcccgagcg acttgtcaac gaaatttttg ggggcacata
     4381 acgagtggct gaggacgcat ggcagacaga caaagacaaa tcatcgcgat cgggggcggc
     4441 ggattctatc gcgattcgga aaatctcggc ttggagcggt acattgttca acagacgggt
     4501 acggcggatc cgagagtggc gtttgtgccc actgccagcg ctgagccgga taactatctt
     4561 gttagttttt acacggcgtt tttgaaactc ggttgccgtc cgtcgcatct gtcgttcttt
     4621 aagagaactg cggatctgcg agcctatctc ttaaaccagg atgtgatctt tgtcggtggt
     4681 ggcaatacga aaaccatgct cgcggtttgg cgcgagtggg gagtgccgga aattctccgc
     4741 gaggcgtggg aatcagggat cctgctgacg ggggtgagcg ccggcgcgat ttgctggttc
     4801 gagcagggag tgacggattc atgggcggg
//
LOCUS       DASSWZ010000163         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1264805, whole genome shotgun sequence.
ACCESSION   DASSWZ010000163 DASSWZ010000000
VERSION     DASSWZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264805"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFN58_04015"
     CDS             <1..988
                     /locus_tag="VFN58_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HET8917190.1"
                     /translation="DLKKVELKPWDRTGGKGAFINMEGAEGTTGAYVLEIAPGKHTSP
                     QRHLYEDLIFVLKGRGATTIWNDKGAKQTFEWQDGSLFALPLNSSYQHFNGQGDESAR
                     FFSVNSMPIVFNLFHNADFIFNTPRDFTDRFNGEAEYFSGKGKAYPGRVWDTNFIADA
                     RNFKLEDWKERGGGGKNVMLEMANGSMAAHISEFPVGTYKKAHRHGPGFNVIIIKGVG
                     FSMFWREGEQPKRYDWQDGSVFTPPAMMWHQHFNTGATGARYLPPRLGGIKYSLGESF
                     GDITKVDKDVKAGGNQIEYYDEDPNIRKMFEEELAKSGTYSRMNPDFYKRPA"
     gene            1522..2115
                     /gene="plsY"
                     /locus_tag="VFN58_04020"
     CDS             1522..2115
                     /gene="plsY"
                     /locus_tag="VFN58_04020"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET8917191.1"
                     /translation="MEVFLLVLFAYLLGSVPTGYILGSLAGVDVRKAGSGNVGATNVA
                     RVVGKRHGIFTLVADIAKGFVPVIVALNLSLSPVATVFVGIAAFLGHLYPVFLRFQGG
                     KGVATALGVFLGLAPWATLVLMAIFVLVLLATRVISLSSMVAAASAPIVFWLFFHSPI
                     LTGMSLFIAAMIVLRHRGNIQRLLSGAEPRLGTSSSR"
     gene            complement(2102..2806)
                     /locus_tag="VFN58_04025"
     CDS             complement(2102..2806)
                     /locus_tag="VFN58_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET8917192.1"
                     /translation="MDVRLKIASVGILFVLTSGCAGGSLSTREKGAGIGALGGAAAGG
                     AIGAAVGRPGIGAAAGGALGLGAGALIGDQLQGQEMKQAEQQKALDQQRAEIAKNQAL
                     IEELKKRNIEARETSRGVMVNLPSVNFQFDSAELTPDGRGRVDQIANIVRREAPNRRV
                     AVEGHASRESAAQEAYNQRLSERRADTVADALARDGVRNSQISSRGLGTRSPIATNDN
                     EEGRRQNRRVEVIIEN"
     gene            complement(2936..3271)
                     /locus_tag="VFN58_04030"
     CDS             complement(2936..3271)
                     /locus_tag="VFN58_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HET8917193.1"
                     /translation="MRQTWIVSLIFLVLTDVGCSGGSLTTREKGAGIGALGGAAAGGA
                     IGAAVGRPGVGAAVGGALGLGAGALIGDQLQGQEIKQAEQQRQIDANQAELDHQRRDL
                     ERLRKQTEY"
BASE COUNT          799 a          956 c          930 g          930 t
ORIGIN      
        1 ggatttgaag aaggttgaac tgaaaccttg ggacagaacc ggaggaaaag gagccttcat
       61 caacatggaa ggcgcggagg gaacaaccgg tgcttatgtt ctggagatag ctccgggtaa
      121 acacacaagc cctcagagac atctttatga agacctgatt tttgttctca agggacgcgg
      181 tgcgacgact atttggaacg acaaaggagc gaagcaaacc tttgaatggc aggacgggag
      241 cctgttcgcc ttgccgctca actcctcgta ccagcacttc aatggccagg gggacgagtc
      301 ggctcgattt ttctccgtca acagcatgcc gatcgtcttc aacctctttc ataatgccga
      361 cttcattttc aacaccccgc gtgatttcac ggatcgtttc aacggcgagg ccgaatactt
      421 tagcggcaag ggcaaggctt atcctggtcg agtgtgggac actaatttca tcgccgatgc
      481 tcgcaatttc aaacttgaag attggaaaga gcgtggcggt gggggaaaga acgtgatgtt
      541 ggagatggcc aatggttcga tggccgctca tatctcagaa tttccggttg gcacctacaa
      601 aaaagcgcat cgtcacggcc ccgggtttaa cgttatcatc atcaaaggag tgggcttttc
      661 aatgttttgg cgagaaggag aacagcccaa gcgctatgat tggcaggacg gcagcgtttt
      721 cacaccgccg gcgatgatgt ggcatcagca ctttaatacg ggtgctacag gggcgcgtta
      781 tctgccgccg cgtttgggtg gcattaagta cagcttggga gaaagcttcg gcgatatcac
      841 caaagtggat aaggatgtca aggcgggcgg caaccagatc gaatactacg acgaggatcc
      901 caacatcaga aagatgtttg aggaggaatt agcgaagtcg ggaacctaca gccgcatgaa
      961 tccggatttt tacaaaagac cagcataact cccaagttct tggaggctga ttctttccga
     1021 gccccgcacc gcttcaaagc ggttcggggt ttttttttgg tggacggagc ttgtgggatt
     1081 cacgaaagaa agattgagag cccggctcac actgtgctaa attcaattaa tggtcggtgt
     1141 gaaccatgtt ttactcccct ttgtccttgt ctgcttagta gcgcttctat gggatgaatc
     1201 cgggcttgaa tcctcagata agaacacagc gaccgataac accgccgttt tggcgtctgc
     1261 ctccacaccc aagccaaccc agaaacagga gagctctact tcgacttcgc cagttgcgga
     1321 gcaaaaagcc cctgccgaca aatctccgtc gatcatcgag atgctcgaag ggcgtgagaa
     1381 cgacttgttt ctctgggtat caatagccgc ggtcgccttt gctattggtt ggattttcgg
     1441 ggggaattac tacttgcgtc gcgatcgtgc gcgcagcaaa aaattacgct tttagtatcc
     1501 agcgaatgac gcttcaagac tatggaagtt tttttgctgg ttctctttgc ctatctgctc
     1561 ggctcagtgc ctacgggtta cattctcggt tctttggcgg gggtcgatgt gcgcaaggcg
     1621 gggagtggca atgtcggggc gaccaatgtc gctcgagtgg tcggcaaacg tcacggaatc
     1681 ttcactctgg tcgcggacat cgcgaaaggc tttgtccccg taatcgttgc acttaactta
     1741 agtttgagtc ccgtggcaac ggtgtttgtt gggatcgctg ccttcctggg tcatctgtat
     1801 ccagtgttcc tgcggttcca gggcggcaag ggagtggcca cggctctggg ggttttcttg
     1861 gggttggcac cttgggctac cttggtcttg atggcaattt ttgttctggt ccttctggcg
     1921 acccgcgtta tctctctaag ttcgatggtg gctgccgctt cagcccccat cgtattctgg
     1981 ctattttttc actctccgat attgacaggc atgagcttat ttattgccgc tatgattgtg
     2041 ctgcggcacc gtggtaacat tcaacgcctg ctgtccggtg cggaaccgcg gctaggcacc
     2101 tctagttctc gataattacc tcgacccgcc gattttgccg gcgaccctct tcgttatcgt
     2161 tggtagcaat cggcgagcgc gtccccaacc cacgtgagct gatttgacta tttctaactc
     2221 catctctagc taaagcatcg gccacagtgt ccgctcgcct ttcggagagt cgctggttat
     2281 aggcttcttg agcggcgctt tctctgctcg cgtggccttc aaccgcgacc cgccgattcg
     2341 gtgcttcgcg cctgacgatg ttcgcaatct ggtcgacgcg gcctctcccg tctggcgtta
     2401 attccgcgct atcaaactga aaattaacgc tcggaagatt gaccatgaca cctcgactgg
     2461 tctcacgagc ttcgatgttg cgctttttga gttcttcaat caacgcttga ttcttggcga
     2521 tttcggcgcg ctgttgatcg agggcttttt gctgttcggc ctgcttcatt tcctgacctt
     2581 ggagctgatc accgatcagt gcaccggcgc ccaaccccaa cgcgccgccg gccgctgcgc
     2641 caatccctgg acgacccacc gccgcgccaa tagccccgcc cgcggcggca cctcctaagg
     2701 caccgatgcc cgcgcctttt tctcgagtgc ttagtgatcc gcccgcacat cccgaagtca
     2761 aaacaaataa aattcctact gaagcgatct ttagacgcac atccataaaa ttgtacctcc
     2821 tacagcgtcc tgtctagaca ccattttctt taccctttgc tgcacagcta tgtttacccg
     2881 ttcagtcctt ttagacgttc gaatttggcc ttcagagata ggcagattct gaaaactaat
     2941 attctgtttg ttttcttagt ctttcgaggt ctcttctttg gtggtccagt tcagcttggt
     3001 tggcatcaat ctgccgctgt tgttcggctt gctttatttc ctgaccctgg agctgatcgc
     3061 cgatcagggc accggcaccc aaccccaaag cgccgccgac cgccgcgcca acgcctggac
     3121 gacccaccgc cgcgccaatg gccccgcccg cggctgcacc tcctaaggca ccgatacccg
     3181 cgcctttctc tcgtgtcgtt aatgatcctc ctgagcaacc aacatccgta agcacaagga
     3241 aaataagaga aactatccaa gtctgtctca taaatcctcc ttcggttcga tccgctactg
     3301 agtcgcgaaa attttaaccg atcgaccctg accgttcaaa cagtccagtt atgcgaaata
     3361 taataatatt ttatttatgc tagtcgctgc gtctggaatc gagaaaaaac acaaaagagt
     3421 tgcggggctt caagctacat cggaatgtcg aatttatgag ttaacttggc tggaaaatag
     3481 ctggggcagg atgacaacga gagatcttga tatcgaagac ttatattgac atcgtagacc
     3541 ttagaagtct atagtttttt atgaaaggac aaccgtggaa actgttacac aggaacaagt
     3601 tttagcggcg ttgag
//
LOCUS       DASSWZ010000164         7436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_160969, whole genome shotgun sequence.
ACCESSION   DASSWZ010000164 DASSWZ010000000
VERSION     DASSWZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160969"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFN58_04035"
     CDS             <1..444
                     /locus_tag="VFN58_04035"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET8917194.1"
                     /translation="EVTEATLKVTFAELFEQRIYLEGMILKPSMVVSGKDNPRQAGVE
                     EVAERTLRCLKRTVPAAVPGIAFLSGGQSAESATEHLNAMNAMGPHPWQVSFSYARAL
                     QDPALKAWKGEAANVPAAQKIFYHRAKCNSAARSGQYNRQMEAAA"
     gene            518..931
                     /locus_tag="VFN58_04040"
     CDS             518..931
                     /locus_tag="VFN58_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET8917195.1"
                     /translation="MSKQLRGSFIKHKGGCHCGGVTFEVEAPVEIEAYECNCSICSKS
                     GFLHLIVPKSRFTLIQGSESLSTYTFNTGVAKHLFCKMCGVKSFYIPRSHPDGYSVNV
                     RCLEPATIRKITVRPFDGKNWEDHALDLDPLSSKS"
     gene            1005..1220
                     /locus_tag="VFN58_04045"
     CDS             1005..1220
                     /locus_tag="VFN58_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917196.1"
                     /translation="MDIEGVGFIIGLIGAIWMKSSMNLIRGYQEQPELKMIGRERENP
                     ALKAFIRGLSLVLFGLGIETYARLFLN"
     gene            1386..3083
                     /locus_tag="VFN58_04050"
     CDS             1386..3083
                     /locus_tag="VFN58_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917197.1"
                     /translation="MDYDLLVRRGRVVDGSGLPSFIADVGVKGGKVAEVGKLKGTAAR
                     TIDADGLAVSPGFIDHHTHLDAQMLWDPYGTCEPQHGITTVVMGNCGLTLAPVKNGDE
                     DALVKSFVRVEAIPRFALEQGVKWGWHSYGEYLDNLEGKVGINVGGLVGHIALRHNVM
                     GEEAVERKATGKEVQQMRGLVLEAMEGGALGISTNRNERHMREDGKPVASRLADDEEL
                     FALCDVLAERNGGTIETILGRNKIEHFEFYRELAQRTQRPVLWQSLQHRWSEPNLWRE
                     QLAAVEPIFRDGYQAYGLSHTVPLVRHFELKNCQVFDEFPTWKNLMFLPVEVRKQAFA
                     DPETRQKLRADLADPRPTNFHRRWDIVRVEKVVKPENQKYAGKTIAELGGMRNQDPLD
                     AFLDLSLEENLGTVFWNANNGGDSNAMGEILRSPYVLVGTSDAGAHVQFGADFGYGTI
                     LLGLWVRERQVMTLEQAIHKLTFHVASIFGLQDRGLLRPGYAADLAIFNPNTVNSHEP
                     EWANDYPAGSKRLIQRSEGMHYTIVNGTVIHEDGRMSGELPGQVLRGPLYREQKAAA"
     gene            3087..3404
                     /locus_tag="VFN58_04055"
     CDS             3087..3404
                     /locus_tag="VFN58_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8917198.1"
                     /translation="MGVTYIPVARVGELAAGAMKEVDLGGRQVLLAFVEGKYFAFSRQ
                     CPHEEADLKTGTLEGGKVRCTNHNYCFDLRTGECVLPKGGPPLTVLPAEERGAEVCIR
                     LEW"
     gene            3650..3787
                     /locus_tag="VFN58_04060"
     CDS             3650..3787
                     /locus_tag="VFN58_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917199.1"
                     /translation="MGVAGLVLGLGVLVFSEMKSVYLWAFLFGLFWVAGLGIFQAKEK
                     T"
     gene            3958..4854
                     /locus_tag="VFN58_04065"
     CDS             3958..4854
                     /locus_tag="VFN58_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HET8917200.1"
                     /translation="MIESQSRRIIGMGFPLLLLFVLFAVAGEAHAQDKSFLWRVQSDK
                     GNIYILGSVHFLKKENYPLNKTIEKAFDSTQKLVLEIDLKSEDAGTIQRVALEKGINR
                     DRTLQQNVSPETYSLAEKRAQELSIDIRALSPLKPWVVALTMTALQLQKLGFDPNYGV
                     DRYLAERAVKSGKTMVGLETAAFQIGLIDQLPERDQESMLRQSLKEMDLLDRGLDQIV
                     RAWSTGDVSMLEGLLLSGKREYPAVHQTIIVDRNRRWLPRIEKMIEQGESALIAVGAA
                     HLVGKDGVIELLRARGYTVEQM"
     gene            4875..5372
                     /locus_tag="VFN58_04070"
     CDS             4875..5372
                     /locus_tag="VFN58_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8917201.1"
                     /translation="MKQIRVADWMTETVLAVETFDSISIARQLMAKHRINQLPVLDND
                     VLVGIVTDRDLRDAYPTSMMIDRGKAIDRFADSVTVEEVMSHDVFTVRPDTPLKKAVG
                     LLRRHRIGSLPVVRNRSLIGIITRSDILDFVLSGLSVRTPASRNRNNKKTTSVGRRTP
                     GKPRV"
     gene            5451..5942
                     /locus_tag="VFN58_04075"
     CDS             5451..5942
                     /locus_tag="VFN58_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET8917202.1"
                     /translation="MASFDIVSRIDLQEVDNAVNITKKAILSRYDFRQSKTEITLDKK
                     EKKIRVTTEDDMKMKAVQDTLIENLVRRKVDRKCLDAKESHMASQGMIQREITIKEGV
                     DSDTARNIVKMIKEQKLKVQAAIQENQVRVTGKKIDDLQTVIQILRGANIPIPLQFIN
                     LQS"
     gene            6009..6407
                     /gene="msrB"
                     /locus_tag="VFN58_04080"
     CDS             6009..6407
                     /gene="msrB"
                     /locus_tag="VFN58_04080"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011613411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET8917203.1"
                     /translation="MMEKIKKNDAEWKKELTDEQFHITRKKGTEPAFTGEYWNNHEKG
                     IYRCRCCGNELFSSDTKFDSGTGWPSFWAPVSKENVTAEEDNSLFMRRTEVLCSKCDA
                     HLGHVFDDGPQPTNLRYCINSASLKLDKPK"
     gene            6423..6878
                     /locus_tag="VFN58_04085"
     CDS             6423..6878
                     /locus_tag="VFN58_04085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET8917204.1"
                     /translation="MGERAFQDQGSVSHCHGCGADNEKGLQIKSFWDGDEAVATWNAQ
                     PHHCGGSKDIVNGGIVASLIDCHSLNLAIAQAYRVEGRPIGSLPRIGYVTRNLNVSYL
                     KPAPIGEPLHLKARITKIEGRKAWVSCTLSAAGQVCATGEVLGIRVRRD"
     gene            7016..>7436
                     /locus_tag="VFN58_04090"
     CDS             7016..>7436
                     /locus_tag="VFN58_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8917205.1"
                     /translation="MEYTTLGKTGLKVSVVGLGCGGPSRLGLRDNADSQERAIGIVRQ
                     AIDLGVNFIDTAESYETEGIVGKAIAGIPRDQLVISTKKTLPGAGQTNPENQIKKGLE
                     QSLKHLGTDCIDVYHLHGVEPKDYDFARDRLMPAMRRL"
BASE COUNT         1804 a         1830 c         2208 g         1594 t
ORIGIN      
        1 gaagtcaccg aagcaacttt aaaagttacg tttgcagaac tttttgaaca gcgcatttat
       61 ctcgagggaa tgattctcaa gccaagcatg gtggtatcgg gaaaggataa tccgaggcag
      121 gcaggtgttg aggaagtcgc tgaacgcacg cttcgctgct taaagcggac agtcccggcg
      181 gcggtcccag ggatcgcgtt cctttcgggt ggccagagcg ctgaaagcgc gacggagcat
      241 ctcaatgcga tgaacgccat gggtccccat ccgtggcaag tgagcttctc gtatgcgcgc
      301 gccttacagg atccggcatt gaaagcgtgg aaaggcgagg ctgccaatgt tccggcggcg
      361 cagaaaattt tctatcaccg cgccaagtgc aacagcgccg cccgaagcgg tcagtacaat
      421 cgacaaatgg aagcggctgc gtaacccgct gccttaaaga tttggttcga tgccgtgccg
      481 cgcgcctgct agcatgcatt tgcgatgggg agtcgcgttg agtaagcaat tgcgagggtc
      541 attcattaag cacaagggtg gatgccattg cggcggggta acgtttgaag tcgaagcgcc
      601 ggtggagata gaagcgtacg aatgcaactg ttcgatctgc agcaagagcg gctttctcca
      661 tctcatcgtg ccaaaatctc gattcactct catccaaggt tcagaatcgc tttcaaccta
      721 cacattcaat accggtgttg ccaagcatct gttttgcaaa atgtgcgggg tcaaatcctt
      781 ctatattcct agatctcatc ccgacggtta cagtgttaat gtgcgctgcc tggagccggc
      841 tacgatcaga aaaattactg tgagaccctt tgatggcaag aattgggagg atcacgctct
      901 cgatcttgac cctctgtcca gcaagagctg agtgaaaatt tgggagttcg aatcgtttaa
      961 atattgacct gttcggattg ttctagaacg cgggagcgaa cgccatggac atcgaaggtg
     1021 tgggctttat catcggcttg atcggcgcta tctggatgaa aagctcgatg aacttgatca
     1081 gaggatacca ggaacagccg gaactgaaaa tgatcggtcg cgaaagggaa aaccctgcgc
     1141 tgaaggcctt tatacgaggt ctctccttag tgctcttcgg acttgggatc gaaacctacg
     1201 cccgcctgtt tttgaattag tcaaatcgtc cctctcggtg gagcgtatgc tagcgtcgtt
     1261 atgttgacac ggcgtctggt tatttcgtat gttgcgagaa acaataagcg atgcgaacga
     1321 catcagcgtt gactaacttc aaaccgccaa acaaactctg cacaaagtaa cgaggaggat
     1381 caaaaatgga ttatgacttg ctagtgcgtc gtggccgcgt tgtggatggc tccggactgc
     1441 cctctttcat agccgacgtg ggagtcaaag gcggcaaggt cgccgaagtg ggaaaactca
     1501 agggaacggc cgctcgtact attgacgcag atgggttggc ggtctcgccg ggatttatcg
     1561 atcaccacac ccatttggac gcccagatgc tttgggatcc gtacggcacc tgcgagccac
     1621 agcacggcat caccacggtc gtgatgggca actgcgggtt gactctagcg ccagtgaaaa
     1681 acggcgatga agacgcgttg gtaaagagct tcgtgcgggt cgaagcgatt ccccgttttg
     1741 ccttggagca gggagtcaaa tggggctggc atagctacgg cgaatatctc gataacttag
     1801 agggcaaagt cggcattaac gtcggcgggc tggtgggcca tatcgccctt cgccacaacg
     1861 tgatgggcga agaggcggtg gagcgtaagg cgactggaaa agaagtccag cagatgcgcg
     1921 gtctcgtcct ggaagccatg gaagggggcg cgttgggaat ttccacgaat cgcaatgagc
     1981 gtcatatgcg tgaagacggc aagccggtgg ccagccgatt agccgacgac gaagagcttt
     2041 ttgcgctctg cgacgtgttg gcggagcgca acggcggaac gatcgagacc atcctgggcc
     2101 gcaacaaaat cgagcacttt gaattctacc gcgagctggc acagcgcacc caaagaccgg
     2161 ttctctggca aagcctgcaa catcgctggt ctgagcccaa tctctggcgc gagcagcttg
     2221 ccgccgtgga accgattttc cgcgatggct accaggccta tgggttatcc cataccgtac
     2281 cactggtccg ccacttcgag ctcaagaact gtcaagtttt cgacgaattc ccgacgtgga
     2341 agaacttgat gttcctgccc gtggaagtgc gcaagcaagc gtttgccgat cccgaaaccc
     2401 ggcagaagct gagggcggac ctggcagacc cgcgcccgac gaatttccat cgccgctggg
     2461 acatcgttcg cgtggaaaag gtcgtgaaac ctgaaaacca gaaatatgcc ggcaagacca
     2521 ttgcagagtt aggcgggatg cgtaatcaag atccgctcga tgccttcttg gatctgtcgc
     2581 tggaagagaa tctgggcacg gtcttttgga atgccaataa tggcggcgac agcaacgcga
     2641 tgggcgagat tctccgcagc ccctacgttc tcgtcggaac ttccgacgcg ggcgcgcacg
     2701 ttcaattcgg cgctgatttc ggatacggca cgatcctctt gggtctgtgg gtgcgcgagc
     2761 gacaagtgat gaccttggag caagccattc acaagcttac cttccacgtc gcgtcgattt
     2821 ttgggttaca ggatcgcggt cttctgcgtc caggctacgc ggcggatctg gcgatcttca
     2881 atccgaacac ggtgaattct catgagcctg aatgggccaa cgactatccg gcgggaagca
     2941 agcggttgat ccagcgctcc gaaggcatgc actatacgat cgtcaacggc acagtgatac
     3001 acgaagacgg ccggatgagc ggcgagttgc cgggacaagt tcttcgcggt ccgctctacc
     3061 gggaacaaaa agcggcggct tgacggatgg gagtgactta cattccggtg gctcgggtgg
     3121 gtgagttggc ggcgggagcg atgaaagagg tcgatctcgg cgggcgccaa gttcttctgg
     3181 cgtttgtcga gggaaaatat ttcgcattct caaggcaatg tccgcacgaa gaagcggatc
     3241 tcaagaccgg gacactcgaa ggcggcaagg ttcgctgcac caatcacaat tattgcttcg
     3301 acctccggac cggcgagtgt gttttgccca aagggggtcc gccgttgacg gttctcccgg
     3361 cggaggagcg gggcgccgag gtttgtatcc ggctggagtg gtagagagtc agacagagtg
     3421 ccggcatagt ctcaagaatt ctcgatttgg ccggggagac gccatcagtg tctccccgtt
     3481 gtcttggttt tctcaagagc agtggacctg tcacccgccg aaagtctagc caaacactcc
     3541 aataaaaggt aaattttcag attcggctcg ccgcttctgg cgctgagtgc agatttttga
     3601 catgactcaa ggcaatctag gtttccggga aaggcggaaa agatttatca tgggggtggc
     3661 cggtttggtc ctgggactcg gggtactggt gttcagtgaa atgaaatcgg tttacctctg
     3721 ggcatttctg tttgggcttt tttgggtcgc gggcttgggg atttttcagg ccaaagaaaa
     3781 gacgtgagtc attctcgcgg cgcgaggaat gcgcaatctt gactccggtg aagagaggat
     3841 cgaagactcg ggaacaaaag cggttctacg cacaatcgcg cgcaagattc acgtcgcagc
     3901 tttgcttgct tcattggtgt tgaccggaat cgcactcgtc gtattaagag gaatctcatg
     3961 atcgaatcac aatccaggcg catcatcggt atgggttttc ctttgctgct tctgttcgtg
     4021 ctctttgcgg tcgccggtga agcgcacgcg caagacaaga gctttttgtg gcgggttcag
     4081 tccgacaaag gtaatattta tatcttgggt tccgtgcatt ttttgaagaa ggaaaactac
     4141 ccgctgaaca agacgataga aaaggctttt gacagtaccc agaaactcgt gctggagatc
     4201 gacctcaaaa gcgaggatgc cgggacgatt cagcgtgtgg ctctcgaaaa aggcatcaat
     4261 cgtgaccgca ccctccagca aaatgtttcc ccggagacct acagtctcgc ggaaaagcgc
     4321 gcgcaagaac tgagtatcga tatccgcgcg ctgagcccgc tgaagccctg ggtggtggcg
     4381 ctgaccatga ccgcgttgca actgcaaaag ctcgggttcg atcctaacta tggcgtcgat
     4441 cgttacctgg cggaacgcgc ggtaaaatcg ggcaagacaa tggtgggact ggaaaccgcc
     4501 gcattccaaa ttgggctcat cgatcaattg cctgagagag accaggaatc gatgctgcgt
     4561 caaagtttga aagagatgga tctcctggac agggggctgg accagattgt tcgtgcgtgg
     4621 tccacggggg acgtctctat gttggaaggg ttattgctga gcggtaagcg ggaatacccg
     4681 gcggtccatc agacgatcat cgtcgatcga aatcggcggt ggttgccgcg gatcgagaaa
     4741 atgatcgagc agggtgaaag tgcgttgatc gcggtgggag cggcgcatct cgttggcaaa
     4801 gatggggtca ttgagttatt aagggcgcga ggctataccg tggagcagat gtgatgcagt
     4861 ccggagagca caagatgaag caaattcgtg ttgctgattg gatgaccgaa accgttctcg
     4921 ccgtcgaaac cttcgatagc atcagcatcg ctcgccaact gatggccaaa caccgcatta
     4981 accagctgcc ggttctcgat aatgacgtgt tggtcggaat agtgaccgat cgtgatttgc
     5041 gcgacgccta tcccacgagc atgatgatcg accgcggcaa agcgatcgac cggtttgccg
     5101 attccgtgac cgtcgaagag gtgatgagcc acgatgtatt caccgtgcgt ccagacacgc
     5161 cgctcaaaaa ggcggtcggt cttttgcggc gccatcggat cggttccttg cccgtggtta
     5221 ggaacaggag cttgatcgga atcataacga ggagcgatat tttggatttt gtgttgagcg
     5281 ggctgagcgt gcggacgcct gccagcagga acagaaacaa caaaaaaacc acatcagtcg
     5341 gccggcgaac acctggaaaa ccgcgtgttt agagttttgg gacgtactta cgggttgccg
     5401 agacaatgct tgcaggctgc ttcggcggcc tgtcggggga gaaccgagtc atggcgtcct
     5461 tcgatatagt cagccggatc gatctccagg aggtcgacaa cgcggttaat atcaccaaga
     5521 aagcaatcct gtcgcgctac gattttcgcc aatcgaaaac ggagatcact ctcgacaaga
     5581 aggaaaagaa gatccgtgtt accaccgaag atgatatgaa gatgaaagcc gtacaggata
     5641 cgctcattga aaacctggtc agacggaaag tcgaccgaaa gtgtctggac gccaaggaga
     5701 gccatatggc gtcacagggc atgatccagc gtgaaataac aatcaaggaa ggggttgact
     5761 ctgacaccgc tcggaatatc gttaaaatga tcaaagagca aaagctaaag gtccaagcgg
     5821 ccatccagga gaatcaagtc cgggtgacag gtaagaaaat tgacgatctt cagacggtga
     5881 ttcaaatact acgcggagcc aacattccga ttccgctcca gtttatcaac ctgcagagct
     5941 gactgaagct gttgtctcag gatggacatc aaagagtgtc cgtggtttca accacgcgcg
     6001 aggagaaaat gatggaaaag attaaaaaga atgacgccga gtggaagaaa gagctgacgg
     6061 acgagcaatt tcacatcacg aggaagaaag gcaccgagcc cgcgttcacc ggagagtatt
     6121 ggaataatca tgagaagggc atctaccgtt gccgctgctg tggcaatgag ctctttagct
     6181 cggataccaa gtttgattcc ggaaccggtt ggccgagttt ttgggccccg gtgtcgaaag
     6241 aaaatgtcac cgctgaagag gacaatagcc tctttatgcg tcgaaccgag gtcctgtgca
     6301 gtaagtgcga tgcccatttg ggtcatgtct tcgacgacgg tccacagccg acgaatctcc
     6361 gttactgtat caattccgct tcgctgaagc tggacaaacc gaaataacga ctgctgcggt
     6421 gtatggggga acgggctttt caggaccagg gctctgtcag tcattgtcac ggctgcggcg
     6481 cggataacga gaagggcttg cagatcaaga gcttctggga cggagacgag gccgtcgcca
     6541 cctggaatgc ccagccgcat cactgtggcg gaagcaaaga tatcgtcaac ggcgggattg
     6601 ttgcgtcact gatcgactgt catagcctca atttagcgat tgcgcaggcc tatcgtgttg
     6661 agggccgccc catcggcagc ttgcccagga ttgggtacgt cacgagaaat ttgaacgtct
     6721 cttatctgaa gccggccccg attggcgaac ctttgcatct gaaagcccga atcacaaaaa
     6781 ttgaaggacg taaagcttgg gtgagttgta cgctgagtgc ggcgggacaa gtgtgcgcga
     6841 ccggggaggt gttgggaatc agggtgcggc gcgattaaga tctcgcgcgg ttcaagggtt
     6901 caaaagttca aagtttaaag cgtatcttcc tctcattggg gattgaacct tgagaacctt
     6961 gagctttgaa tcgctcggct atctgcggcg cagacgattt agaaagaaag ccacgatgga
     7021 atacacaacc cttgggaaaa ccggactcaa agtaagcgtg gtcggccttg gttgcggtgg
     7081 ccccagccgc cttggactgc gcgacaacgc ggactcgcag gagcgcgcca tcggaattgt
     7141 ccgacaggcg atcgatttgg gtgtcaactt tatcgacacg gcggagtcct atgaaaccga
     7201 gggaattgtc ggcaaggcaa tcgcgggaat tccgcgcgat cagctcgtta tctccaccaa
     7261 aaagactctc ccgggcgctg gccaaacaaa tcccgaaaac caaatcaaga aaggtttgga
     7321 gcaaagtctg aaacacctcg gcacagattg catcgacgtc tatcatctgc acggtgtcga
     7381 gcccaaggat tatgacttcg ccagggaccg gctcatgccg gcgatgcggc gcttgc
//
LOCUS       DASSWZ010000165         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_33651, whole genome shotgun sequence.
ACCESSION   DASSWZ010000165 DASSWZ010000000
VERSION     DASSWZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33651"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..545
                     /locus_tag="VFN58_04095"
     tRNA            470..545
                     /locus_tag="VFN58_04095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:503..505,aa:Lys,seq:ttt)
     gene            551..628
                     /locus_tag="VFN58_04100"
     tRNA            551..628
                     /locus_tag="VFN58_04100"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Glu,seq:ttc)
     gene            complement(645..>2301)
                     /locus_tag="VFN58_04105"
     CDS             complement(645..>2301)
                     /locus_tag="VFN58_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET8917206.1"
                     /translation="IHPGGFLLGHEPVHDIVPIENGAMPDRTVIQWDKNDLDDLGLFK
                     VDLLGLGGLTQLDLCFKLLRQHRAIEYSLATIPPDDSAVFNMICKSDTVGVFQIESRA
                     QMAMLPRLKPKNFYDLVIEISIVRPGPITGGMVHPYLRRRSGKETEEYPHPSLEPVLK
                     KTLGVPLFQEQVMRLAVVAADYTPGEADQLRRDMAAWHRTGRMERHRERLITRMQAKG
                     IAPQFAERVFEQIRGFGEYGFPESHAASFALIAYATAWLKCHYPAEFTCALLNAQPMG
                     FYASATIVEDGKRHHVIVQPVDVAVSEWDCTLEPCEESAGGFAVRIGLRYVKGLGERD
                     WESIAAARRAAPFASVGDFVRRTTLDEGVLGTLAEAGAFDAFNLARRRALWDVRRLAR
                     TREESLSLQARESIPLFDSLSAFEEVKWDYRTTSHSPRRHPLEPMRPSLNRQGLPAAR
                     TVASMKNGEKVHYAGLVICRQRPGTAGGVVFMTLEDETGFVNVVVWESVFQRYSVLAK
                     TVSFLGITGTLQVEDNVVHLVAEKLWEPKLELKPTGAPSRDFH"
BASE COUNT          507 a          668 c          588 g          538 t
ORIGIN      
        1 ggaatcctgt gctctatcct actgagctac gggggcacag ggcccaagct atcgtattgc
       61 tatcttcaaa actcgtcgct tctttgttat catatttcct caaatactgg tcaaatcttc
      121 agcctcatcg gcatgattca tcttaatctc ttcctccgcc accaaagttt ttgtgtcgct
      181 acgattttag ccgttttgat ttcaggctgt gttgtctctg attccccgtt aagtatcagt
      241 ctttacaatc ccaagacagg cgttcagcgc acatgctccg cgaaagaatc atcgtcgtcg
      301 tcgaaagatg tttcggcgct ttccagcgca gtggaaacgt gcgcgaagca attggaagcc
      361 cgcggattcg tcagaagcaa taatcactaa acgcggcaat tgcttcactt tactttcatt
      421 gacttaccgc ggtattcccg ttatgtagaa tgggtttaca tccgccagcg agccgttagc
      481 tcaggcggta gagcatctgc cttttaagca gagggtcgct ggttcgaatc cagcacggct
      541 caccatttgc gtccccgtcg tctagcctgg cccaggacat cggcctttca cgccggcaac
      601 acgggttcaa atcccgtcgg ggacgccaaa aaccgtgttt cgttttaatg gaagtcgcgg
      661 ctgggcgcgc ctgtcggttt gagctccagt ttcggctccc agagtttctc cgcgaccaga
      721 tgaacgacat tatcttcaac ctggagagtg ccggtaatgc ctaaaaaact cacagtcttt
      781 gccaatacag aatagcgctg gaagacgctt tcccatacga caacgttcac gaacccagtt
      841 tcgtcttcca aggtcatgaa gacaacaccg ccggcggttc ccggtcgctg acgacagatg
      901 accaggcctg catagtgaac tttctcaccg tttttcatgg acgccaccgt gcgagcagcc
      961 ggcaaccctt gccggttaag gctcgggcgc ataggttcaa gcgggtgacg gcgcgggctg
     1021 tgtgaggtcg tgcgatagtc ccacttcacc tcttcaaatg cactcaagga atcgaacaga
     1081 ggtatgcttt cgcgtgcctg aagagaaagt gattcttccc tcgtacgagc gagccggcgc
     1141 acgtcccaga gcgctctgcg ccgagcaagg ttgaaggcat cgaaggcacc ggcttcggcg
     1201 agcgtgccaa ggaccccttc gtctaatgtc gtgcgacgta cgaaatcacc gactgaggcg
     1261 aacggtgcag cgcgccgcgc cgcggcgatg ctctcccaat ccctttcgcc taaccccttc
     1321 acgtagcgca gtcctatgcg taccgcgaag ccacctgcgc tttcttcgca gggctccaat
     1381 gtacagtccc attcactgac cgccacgtcg acgggttgca cgatcacatg gtgacgcttt
     1441 ccgtcctcta cgatcgtcgc cgacgcgtaa aagcccatgg gctgggcgtt gagcaacgcg
     1501 caagtaaact ccgcagggta atggcacttg agccaagccg ttgcgtaagc gatgagtgcg
     1561 aaactcgctg catggctttc aggaaagccg tactcgccga agccgcgaat ctgctcaaaa
     1621 actcgttcgg caaattgagg cgcgattccc ttcgcctgca tgcgggtgat cagccgctcg
     1681 cgatgacgct ccatccttcc ggtgcgatgc cacgcggcca tgtcgcgcct aagctggtcc
     1741 gcctcacccg gggtgtagtc cgcagcgacc actgccaaac gcatgacttg ctcttgaaag
     1801 agcggtacac ccagggtttt tttcaacacc ggttccaaac tcggatgagg atattcctct
     1861 gtctccttac cgctacggcg gcgtaaatag ggatgaacca ttccgccggt aatgggtccg
     1921 ggtcggacaa tgctgatttc gatcaccaga tcataaaaat tcttcggctt gagtctcggc
     1981 agcatagcca tctgcgcccg gctttcgatt tgaaaaactc ccacggtgtc gcttttacag
     2041 atcatattga agaccgcgga atcatccggc ggaatcgttg cgagggaata ctcgatggct
     2101 ctatgctgtc ttaaaagctt gaaacagaga tcaagctgag taagcccgcc aagacccaga
     2161 agatcgactt taaaaagtcc taagtcgtcc agatcatttt tgtcccattg aataaccgtc
     2221 ctgtccggca tagcgccatt ttcaatggga acgatatcgt gcaccggctc atggcccaat
     2281 aggaatcccc cggggtggat t
//
LOCUS       DASSWZ010000166         9471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_967501, whole genome shotgun sequence.
ACCESSION   DASSWZ010000166 DASSWZ010000000
VERSION     DASSWZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967501"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFN58_04110"
     CDS             <1..400
                     /locus_tag="VFN58_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8917207.1"
                     /translation="IKAAYDSLPFEAEVLPFIEKMDEAYARADLVLCRAGATTVAELT
                     AFGKPAVLVPYPYAIYDHQRRNAQALQDQGAAEMILDQELNGNLLAGRIQAYVADRWK
                     VERMAAAARVMGRPEAAARIVDECYALVRG"
     gene            425..1795
                     /gene="murC"
                     /locus_tag="VFN58_04115"
     CDS             425..1795
                     /gene="murC"
                     /locus_tag="VFN58_04115"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET8917208.1"
                     /translation="MLQKRHRLHFVGIGGIGMSGIAEVLLNLGYAVTGSDLHESEVTE
                     RLRKLGAQVFIGHQEDNLAVDPSVVVISTAVKYSNPEVLEARRRHIPVIPRAEMLAEL
                     MRMKYGVAVAGSHGKTTTTSMIASVLSAGGLDPTIVIGGRVRMLGTNAKMGQGEILVA
                     EADESDGSFLLLSPTIAVVTNIDREHMDFHQTMEKLSESFLDFINKIPFYGLAVLCMD
                     NGNVRALLPKVRKRFATYGLSSDADFSAQDLRMRPAGVDFTALHHGKPLGHVCLHLPG
                     RHSASNALAAVAVGHELQIPFSRVAEALDGFTGIHRRFEIKGEPRGITIIDDYGHHPA
                     EIQVTIEAIHDSWKRPLIVIFQPHRFSRTQDLFDDFLTAFEGADRLVLTEIYAAGEDP
                     IEGVTSEALYQGIKRRGHMEVDFIPDKGQIVKQLVGKLNPGDIVLTLGAGDVYKVGEA
                     LVEALK"
     gene            1899..2861
                     /gene="murB"
                     /locus_tag="VFN58_04120"
     CDS             1899..2861
                     /gene="murB"
                     /locus_tag="VFN58_04120"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET8917209.1"
                     /translation="MKSIMVPMMQESNETALAPELKLISGARIKVSEPLARYTSIKVG
                     GPADFFIEVENDAALTQVLRTLNQYRTGFFLLGNGSNVLISDRGVRGAVIHLAGAFKQ
                     AEWRQEDEIVWVRVGAACGVTQLVREAARRGYGGLEFAEGIPGTIGGALFMNAGAYGS
                     EFEKVIDRVDGFTQEGEPIRLSRNEMTFTYRDSHLPAGMVVTEARLRLHREDAAKVTS
                     RVRELVTKRKTSQPSGYPNSGSMFRNPPEDFAGRLIEAAGLKGKKIGHAQISERHGNF
                     IVNLGGAKGEDVRRLMELARAEVKTRFGVELEPEVRLLGDWATL"
     gene            2876..3691
                     /locus_tag="VFN58_04125"
     CDS             2876..3691
                     /locus_tag="VFN58_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET8917210.1"
                     /translation="MQLTVYRRDNRKRNRRSWRQWIMVGSTFATLIVLSIALYGWRRQ
                     LMVAGAAAREFVLESPYFSVREIQVRGGEKFGGNEIVAMAGLKHGMNIWRIEPATIEK
                     KVAKHPWVRRVLVRREFPRRVVIEIEERTPRAIVAMGKLYYVDSDGVVFKEVGAGESV
                     QFPLLTGLPPDELGSRDPAVRRKIQDAVRLSDLMARDSRTISEIHFEAADRVTLYTTA
                     YPLALHMGWGDWEDKVRRVDRVLKLWKGHEERLVTLDASFGDQVVARVRRMRQ"
     gene            3732..4958
                     /gene="ftsA"
                     /locus_tag="VFN58_04130"
     CDS             3732..4958
                     /gene="ftsA"
                     /locus_tag="VFN58_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET8917211.1"
                     /translation="MAKKHSIVVGLDIGTSKVCAVVGEMTDRGVEIVGIGSHPSQGLR
                     KGVVVNIDSTVNSIKKAVEEAELMAGCEIHTVFTGLSGNHIKGFNSHGIVAVKNKEVC
                     QRDLDRVIDAAKAVAIPTDREVLQILPQDYIIDDQDGIKEPLGMSGVRLEARVHIVTS
                     TVTSAQNIVKCCNRAGLNIGDIILMPLAAAEAVLSGEERELGVALVDIGSGTTDIAVF
                     HDGTVRHSAVLSVGGNHLTNDIAAGLRTPTADAERIKQRYGLAKASTVSGDQRVEVSS
                     VAGKAAGTVSRQILCEIIEPRMEEIFQLVQREIAKSGYEGSLASGVVMTGGSMLLPGA
                     VETAEQVFGMPARLGSPTHVVGLVDVVSSPVYAASVGLVLCGMKRQERAFARFRDDRI
                     LAKVKHRMSDWLSEFF"
     gene            5000..6205
                     /gene="ftsZ"
                     /locus_tag="VFN58_04135"
     CDS             5000..6205
                     /gene="ftsZ"
                     /locus_tag="VFN58_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET8917212.1"
                     /translation="MFEIVEQQENLKARIKVIGIGGGGGNAVNTMIGSKFTGVDFMVA
                     NTDAQSLDASRAPVKIQLGETVTKGLGAGANPEIGRRAAQENEEIIQDYLAGSDMIFI
                     TAGMGGGTGTGGAPVVARLAREAGALTVGVVTKPFIFEGKKRMRQAEEGIEHLKQNVD
                     TLIVIPNQRLLSIAAKTTTMLEAFHRADDVLLQAVRGISDLIITPGLINLDFADIRTV
                     MAEMGLALMGAASAAGENRAIEAAQKAISSPLLEDISIQGARGVLINITGGPDLCLHE
                     VNEAASMIQEEAHEDANIIFGSVIDETMTDEIRITVIATGFGEDREERKPVPVNVSSI
                     ASAPQKNKKVVHLGTIVDDLDTPTWQRKKQGSEETETLTLNKTTFDFGRDKEDDEYDI
                     PTFLRKQMD"
     gene            complement(6309..7397)
                     /gene="ddlA"
                     /locus_tag="VFN58_04140"
     CDS             complement(6309..7397)
                     /gene="ddlA"
                     /locus_tag="VFN58_04140"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET8917213.1"
                     /translation="MKKKLRVAILFGGKSAEHEISVISARNIVEAMDKKKYEIVSIGI
                     DKQGCWFFDEGARLLHSRDQAQVKFERRRNPTAVLPGATQAPLLTPRRRLGKVDVVFP
                     VLHGPFGEDGTVQGILKLANVPFVGAGVLGSAVGMDKDVMKRLLRDAKLPIAKFLVFE
                     RNGASKINFAGVKRALGLPFFVKPANLGSSVGISKVGANKEFGGAITEAFRYDNKILI
                     EESIHGREIECSILGNENPIASLPGEITTRHDFYSYDAKYLDEKGAQLVIPAALPKSI
                     VKAVQGLALRTFKTLACEGMARVDFFLRDNREIFVNEINTIPGFTKISMYPKLWEASG
                     ISYSKLIDRLIQLALERFRREKSLRTSL"
     gene            complement(7473..8651)
                     /locus_tag="VFN58_04145"
     CDS             complement(7473..8651)
                     /locus_tag="VFN58_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8917214.1"
                     /translation="MLKCIFLSITLVLSVSLPATVWAQKGTIKIAVQAPLSGEQAALG
                     EHIKLGAQLAVEEATKAFKALGFDLVFVPYDDQAKPEVGVANARNIVADPNVLVLVGH
                     FNSGVALPASEVYKDAMLAMISPANTATEITDRGYPNVNRVCGRDDVQGPVGARFAAQ
                     DLKRKSVYIIHDKTLYGQGVADNFRNEANKLGLKVFGYEGTEERANFSPIITPMKAKN
                     PDLVYFGGIYHQGGLLLKQMREKGVNAAFMGPDGLDSSEMVKIAGSQVVGSYYTTVAG
                     PPAAYPESAAFATKFKQRFGKEVESFAMYGYDAALVGIKAIEQTLQTNGGKKPSRAEV
                     SAAVRKLKDFKGVTGSIAFDNKGDPIKAKYFVLQFEKRSYPGKVVKVIEQQAPEAKKS
                     "
     gene            8773..9162
                     /gene="acpS"
                     /locus_tag="VFN58_04150"
     CDS             8773..9162
                     /gene="acpS"
                     /locus_tag="VFN58_04150"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET8917215.1"
                     /translation="MVLSVGVDMVEVERIQKALEDSRIGKRFRERVFTANEIQYCEKK
                     RRGRYESYAGRFAAKEAVMKALGRGWGSKVSWLDIEVARARSGKPEIVLHNKTARLAE
                     QLGIGRWSLSITHTEQHGLAFVVAEAQ"
     gene            complement(9204..>9471)
                     /locus_tag="VFN58_04155"
     CDS             complement(9204..>9471)
                     /locus_tag="VFN58_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917216.1"
                     /translation="KSKRQGLVVDFYHLFGYLSGLLFIKPEMDPSTLVRTAALVHILD
                     AILCRVIAGHSGRQKLPWTVAGLFFGIWALGTLFLLPAKKPNYH"
BASE COUNT         2148 a         2403 c         2817 g         2103 t
ORIGIN      
        1 aatcaaagcg gcctatgatt ctctcccttt cgaagcggag gtgcttccgt tcatcgagaa
       61 aatggacgag gcgtatgccc gcgccgactt agttctttgt cgggccggcg ccactacggt
      121 ggcggaactg acggcgttcg gcaagcccgc ggtgctagta ccttatcctt acgcgatcta
      181 tgaccatcag cgccggaatg cgcaagcgct ccaagatcaa ggagcggcgg agatgattct
      241 cgatcaggaa ctgaacggaa atcttctggc tggaaggatc caagcctatg ttgcagatcg
      301 atggaaagtc gagcgcatgg ccgctgcggc ccgggtgatg gggcgaccgg aagccgcggc
      361 tcgaatcgtc gatgagtgct acgctcttgt acgcgggtaa tgtgcggacg gtgataggaa
      421 ttctatgctg cagaaaaggc accgactcca cttcgttggc atcggcggca tcggcatgag
      481 cgggattgca gaagtgttgt tgaacctggg ttatgcggtg accggctccg atctgcacga
      541 aagcgaagtg acggagcggc tccggaagtt aggggcgcag gtgttcattg gccatcaaga
      601 agacaatttg gcagttgatc cttcggtggt ggtgatctca acagcggtca aatactccaa
      661 cccggaagtc ctggaggcgc gacggcgcca catcccggtc atcccgcgcg ccgagatgct
      721 cgccgagctg atgcggatga agtacggggt cgcggtggcc ggtagccacg gcaaaacgac
      781 gacgacttcg atgattgcct cagtgctgag cgccggtggg ttggatccga caatcgtcat
      841 cggtggacgg gtccgcatgc tcggcacgaa tgccaagatg gggcagggcg aaattctcgt
      901 ggcggaggct gatgaaagcg atggcagctt tctattgctt tccccgacca tcgccgtggt
      961 gaccaacatc gatcgagaac atatggattt tcatcaaacg atggaaaagc taagcgaaag
     1021 ctttcttgac ttcatcaaca aaattccttt ctacggcctg gcggtgcttt gcatggataa
     1081 cggaaacgtg cgggcgttat tgccgaaggt aaggaaacgc ttcgcgacct atggcttatc
     1141 ttctgacgcc gatttctcgg cacaagacct acggatgaga cctgctggcg tcgatttcac
     1201 ggctctgcat catggcaaac cactgggcca tgtgtgtctc catctacccg ggcgccacag
     1261 cgcgagcaat gctttggcgg ccgtcgcggt cggccatgaa ttgcaaattc ctttttctcg
     1321 tgtggcagag gcgctggatg gttttaccgg tatccaccgg cgctttgaga tcaaaggcga
     1381 gccgcgaggg attacgatca tcgatgatta cggtcatcat ccggcggaga ttcaggtaac
     1441 catcgaagcg atccatgaca gttggaagcg gccactaatt gtgatctttc agccgcaccg
     1501 cttcagtcgc acccaggact tatttgatga cttcctaacc gccttcgagg gcgccgaccg
     1561 actggttctt acggagatct acgcggcagg agaggatccg attgaaggcg tgacgagtga
     1621 agcattgtat caagggatca aaagacgggg acacatggaa gtggacttta ttccggacaa
     1681 gggccaaatc gtcaagcagc tcgtcgggaa actcaatcct ggtgacattg tgctgactct
     1741 gggggcagga gatgtctaca aagtcggcga ggcattggtc gaggcgctca aatgaggcta
     1801 agccatggaa cggtggcgtg ttggagtgat gcagtactgg aacaatggga aatggcgtct
     1861 ttatcatcac tccatcactc cattcctccg ttactccaat gaaatcgatc atggttccga
     1921 tgatgcagga gagtaacgaa acagctttag ccccggagct gaagctcatt tccggtgcta
     1981 ggattaaagt ttccgagccg ctggcacgct acacgtccat caaggtcggc ggtccggcag
     2041 actttttcat cgaggtggaa aatgatgccg cgttgacaca agtccttagg actctcaatc
     2101 aataccggac agggtttttt ctcctgggta atggcagcaa cgtcctgatc agcgaccggg
     2161 gggtgcgcgg cgctgtgatt catctagcgg gagcgttcaa acaggctgag tggcggcaag
     2221 aagacgaaat cgtctgggtc agggtcggtg cggcatgcgg tgttacgcag ctggtaagag
     2281 aagcggcccg gaggggatat ggcggactgg agttcgccga aggaattcca gggacgattg
     2341 gcggagctct ctttatgaac gctggggcct acggatccga gttcgagaag gtcatcgatc
     2401 gagtcgacgg ctttacgcag gagggtgagc cgatccgctt atccaggaac gagatgacct
     2461 ttacttatcg cgattcacat ttaccggcag gtatggtggt gaccgaggcg cggctgcgac
     2521 tgcaccggga agatgccgcg aaagtgactt ctagggttcg tgaactggtg acaaagcgaa
     2581 aaaccagcca gccttccggc taccccaact cgggctccat gtttcgtaat ccaccggaag
     2641 attttgccgg gaggctgatc gaggccgcgg gtctaaaagg caaaaaaata ggccatgcgc
     2701 agatctcgga acggcacgga aattttatcg tcaaccttgg cggcgccaag ggggaagatg
     2761 tgcgccggct gatggaattg gcgcgcgccg aggtcaagac tcggttcggc gtcgaactgg
     2821 agcctgaagt tcgattgctc ggggattggg cgactcttta ggcaaaggca aaacgatgca
     2881 actgactgta tatcgcagag acaatcgcaa acgaaacagg cgcagttggc gtcagtggat
     2941 catggtgggc agtacttttg cgactctgat cgttctgagc attgcgcttt acgggtggcg
     3001 caggcagttg atggtcgcag gtgctgcggc gcgcgaattt gttttggaga gtccctattt
     3061 ctctgtcagg gaaattcaag tgcgtggagg agaaaaattc ggcggtaacg aaattgtcgc
     3121 catggcgggg ttgaaacatg gcatgaatat ttggcgcatt gagccagcga cgattgaaaa
     3181 aaaagtcgcc aaacatccat gggtgcgccg cgtcctcgtg cgccgcgaat ttccccgtcg
     3241 ggtggtcatc gaaatcgaag agcgaactcc cagggcgatc gtggcgatgg gaaaactcta
     3301 ctacgtggat tccgacggag ttgtctttaa agaggtcgga gccggagaaa gcgtccagtt
     3361 ccccttactc accgggctac ctccggatga gcttggaagt cgtgatccgg ccgtccgtag
     3421 gaaaatccag gacgccgtcc gactcagcga tttgatggcc agggattctc gtacgatttc
     3481 cgagatccat tttgaggccg cggaccgcgt gactctttac acgacggctt atcccctggc
     3541 gctacatatg ggttgggggg actgggaaga caaggtgcga cgggtagacc gtgttctcaa
     3601 gctttggaag ggacatgagg agcgtttggt gactttggat gcgagttttg gtgatcaagt
     3661 agtggcccgc gttagacgga tgcggcagta aacgagagga tgcattaacg cgtaggtgat
     3721 aacacaaggt catggcgaag aaacacagca tcgtagtggg tttggacatc ggtacctcga
     3781 aggtttgcgc cgtcgtcggc gaaatgaccg accgaggcgt ggaaattgtc ggcatcggat
     3841 cccacccgtc gcaaggattg cgcaaaggcg tcgtcgtcaa tatcgacagc acggtcaact
     3901 ccatcaaaaa ggccgtcgaa gaagccgagt tgatggccgg ctgcgagatt catacggtct
     3961 ttactggcct ttcgggaaat cacatcaaag gctttaacag ccatggcatt gtcgcggtta
     4021 aaaacaagga agtgtgccaa cgagacctgg atcgagtcat agatgcggca aaggcggtgg
     4081 cgattccgac ggatcgggaa gttctccaga ttctcccgca agactacatt atcgatgacc
     4141 aggacggcat caaagagcct ttgggaatgt caggggtgcg cttggaggcg agggttcaca
     4201 tcgttaccag caccgttact tcggcacaga acatcgtaaa atgttgtaac cgcgccggtc
     4261 tcaatattgg cgatatcatt ttgatgccgt tagcggcggc ggaagcggtg ctgagcggag
     4321 aagagcgtga gttgggagtg gcattggtgg atatcggcag cggcactacg gatatcgcgg
     4381 tttttcatga tggcacggta agacatagcg cggtgttgag cgtcggcggc aatcatctta
     4441 cgaacgatat cgccgcaggc ctgcggaccc cgaccgctga cgcggaacgt atcaagcaac
     4501 gctacggtct ggccaaagca tccacggttt caggcgacca gcgggttgaa gtttccagcg
     4561 tggcgggtaa ggccgccggc accgtttccc gccaaatcct ctgtgagatt atcgagcccc
     4621 gcatggaaga gatttttcag ctggtgcaga gagagatcgc aaagtcggga tacgagggtt
     4681 ccttggcgtc cggagttgtt atgaccggcg gttcgatgtt gcttccaggg gccgttgaga
     4741 cggcggaaca ggtgttcggt atgccggcac ggctgggtag tcctacccac gtcgttggtc
     4801 tggtagatgt cgtctccagt cccgtttatg ccgccagcgt ggggctggtt ctctgcggta
     4861 tgaaacgcca ggagcgcgcc ttcgcgcgtt tccgcgacga caggattctg gccaaggtca
     4921 agcatcgaat gtcggattgg ttgagtgagt tcttttgatc caatacaaaa ttattgacta
     4981 atttgaagga ggaaagctta tgtttgagat cgtcgagcag caggaaaacc taaaagcccg
     5041 tatcaaggtg atcgggatcg gcggcggtgg cgggaatgcc gtcaatacca tgatcgggag
     5101 caaatttacc ggggttgatt ttatggtggc gaatacggac gcacagtccc tcgatgcgag
     5161 tcgcgcaccc gtaaagattc aactcggtga gaccgtcacc aaggggctcg gtgccggcgc
     5221 caatccggaa atcggccggc gggcggcgca ggagaacgaa gagatcattc aagactatct
     5281 ggccggttcc gatatgattt ttatcaccgc cggtatgggg ggtggaaccg gtacgggggg
     5341 tgccccggtc gtggctcgtt tggctcggga agcgggcgcg ctaaccgtgg gagtggtaac
     5401 gaagccattc attttcgagg gcaaaaagcg catgcgccaa gccgaagagg ggattgagca
     5461 tctaaagcag aacgtagata ccctgatcgt catccccaac caacgtctgc tcagtatcgc
     5521 cgccaagacc accaccatgc ttgaggcttt tcacagggcg gatgacgtct tgcttcaagc
     5581 agtccgtggc atctcggacc tcattatcac tccaggtttg atcaacctcg acttcgccga
     5641 tatacgaacc gtgatggcgg aaatggggct ggcattgatg ggggccgctt cagcggcagg
     5701 ggagaatcgc gccatcgaag ccgctcagaa agcgatctcc agtccgttac tcgaagatat
     5761 ttccattcaa ggtgcgcggg gagtgctcat caatatcacc gggggtccag acctttgtct
     5821 gcacgaggtc aacgaagccg catcgatgat ccaagaagag gcgcatgaag acgccaacat
     5881 cattttcggc tcagtcatcg acgagactat gaccgacgag attcgtatta ccgtcatcgc
     5941 caccggattc ggggaggata gagaggaacg gaagcccgtg ccggtgaatg tctcgagcat
     6001 tgcgagcgcg ccgcagaaaa acaagaaagt ggtgcacttg ggtaccatcg tggatgattt
     6061 ggacacgccc acttggcaac gcaagaaaca gggcagtgag gagactgaga cgctgacctt
     6121 gaataagacc acgtttgact tcgggcgcga caaggaagat gacgaatacg acattcccac
     6181 tttcttaaga aagcagatgg actgaggttg gcggtttctg cgtcgcccga ttgaatcgat
     6241 tgactgcggc ctacccatcg gtccccgagg tattttggct tttccacagt cccggacccg
     6301 tggccgcatc agagcgaggt tctcaaactt ttttctctgc gaaacctctc taaggcaagc
     6361 tggatcaagc gatcgatgag cttgctgtac gagatcccgc tggcttccca cagctttggg
     6421 tacatactga ttttggtgaa gccggggatg gtattgattt cgttgacgaa aatctctcgg
     6481 ttatcccgaa gaaaaaaatc gactcgggcc atcccttcac aggctagggt cttgaaggtc
     6541 ctgagcgcca gtccctggac tgcttttaca atgcttttgg gaagcgcggc aggaatcacc
     6601 aattgtgctc ctttttcgtc gagatactta gcgtcataag aatagaaatc gtgtcgcgta
     6661 gtaatttctc cgggcagtga ggcgatcgga ttctcgttgc cgagaatcga gcactcaatc
     6721 tcccggccat ggatactttc ttcgatcaaa atcttgttat cgtaacgaaa ggcttctgtt
     6781 atagcaccgc cgaattcttt gttcgcaccg accttgctga tgccgacgga ggaaccgagg
     6841 ttggccggtt tgacaaagaa gggcagcccc aacgctcgtt ttacgcccgc aaagttgatc
     6901 ttgctcgctc cattgcgctc aaaaaccaag aatttggcga tcggcaactt ggcgtctcgg
     6961 agcaatcgtt tcatcacgtc cttgtccatc cccaccgcgg aaccaaggac tccggcgccg
     7021 acgaaaggaa cgttggcgag tttcagaatc ccctgaacgg tgccgtcctc gccaaaggga
     7081 ccgtggagca ccggaaagac cacgtccact tttcccaacc tccgacgcgg cgtaagcaag
     7141 ggtgcctgtg tggctcctgg cagtacggcg gtgggatttc tgcgccgctc gaacttaacc
     7201 tgcgcttggt ccctgctatg gagaagccgc gccccttcgt caaagaacca gcatccttgc
     7261 ttgtcgatgc cgatggaaac gatctcgtat ttttttttat ccatcgcctc aacgatgttt
     7321 ctggccgaaa tcacagagat ctcatgttcg gcggattttc cgccgaacag aatggccacg
     7381 cgcagtttct ttttcataag ctcacggttt caaaagatca gcgggcccct gcttcgagga
     7441 ggacccgcag cgatgagggg aggaggatta gcctatgact tcttggcttc gggggcttgc
     7501 tgctcgatga ccttaacgac cttgccggga tagcttcgct tctcgaattg caggacaaaa
     7561 tatttggctt tgatgggatc gcctttgttg tcaaaggcaa tggagccggt aacacccttg
     7621 aagtccttca acttcctgac cgcagccgac acttcagcgc gactcggttt cttcccgccg
     7681 ttcgtttgta gggtttgttc gatggctttg atgccgacca gagctgcgtc gtagccgtac
     7741 atggcgaagg actcgacttc cttgccgaag cgctgcttga atttcgtggc gaaagcggcg
     7801 gactccgggt aggctgcggg cggtccggcg accgtggtgt aataactacc taccacttgg
     7861 ctgccggcga tcttcaccat ctccgaagag tccagaccat ccggtcccat aaatgccgcg
     7921 ttgacgcctt tttcgcgcat ctgcttcaac agcaacccgc cctgatggta gatgccgccg
     7981 aaatagacta ggtccggatt cttcgctttc atcggcgtga tgatcgggga aaagttggcg
     8041 cgctcttcgg tgccctcata accgaagacc ttcagcccga gcttgtttgc ctcattgcga
     8101 aagttatcgg ccactccctg gccgtagaga gtcttatcgt ggatgatgta aaccgatttg
     8161 cgcttgagat cttgagcggc aaatcgcgct cccaccggtc cctgcacgtc gtcacggccg
     8221 cagacgcggt taacgttggg atagcctcga tccgtgattt ccgtcgccgt gttcgcaggc
     8281 gagatcatgg ctagcatcgc atccttatag acttccgaag cgggaagagc gacgccggaa
     8341 ttgaagtgac ccactaaaac cagcacgttc ggatcggcaa cgatgtttct ggcattggcg
     8401 acgccgacct cgggtttggc ttgatcgtcg taaggcacga agaccaggtc aaaccccagg
     8461 gctttgaacg ccttggttgc ctcttcaacg gctaactggg ctcccagttt tatatgctcg
     8521 cccaaagcgg cttgctctcc tgaaagcggc gcctgaacgg caatcttgat ggttcctttt
     8581 tgcgcccaga cggtggctgg caatgaaact gacaggacaa gcgttattga cagaaagatg
     8641 cacttaagca cgatagggcc tctctccttt ggttgagata ccccatgcta acaaatcctt
     8701 atgatcagtg ccactgattt tttgacggga gatttgttga cagcccctcc agcgcggggc
     8761 aaactttgtt tcatggtgct tagtgttggc gtcgacatgg tcgaagtcga acgcattcaa
     8821 aaggctttgg aagattcgcg gatcggcaag cgctttcgtg agagggtttt tacagctaat
     8881 gaaattcaat actgcgaaaa aaaacgacgc ggacgctatg agagttatgc cggacgtttt
     8941 gccgccaaag aggccgtgat gaaagcgctg ggacgcggct ggggttcaaa agtaagttgg
     9001 ctcgacatcg aagttgcgcg cgcccgtagc ggcaagccgg agattgtctt gcacaataaa
     9061 accgcgcggc tcgcggagca gctaggaata gggcgttggt ctctttcgat cacgcacacc
     9121 gagcagcatg gactcgcttt tgtcgttgct gaggcgcaat agctcaagcg tcctgacaga
     9181 ctcgccgtat cgcagagctc tttttagtga taattcggtt tttttgccgg caacaagaac
     9241 aatgttccta aagcccagat cccaaagaac aagcctgcga cagtccaagg aagtttttgc
     9301 cgcccgctgt gtccggcgat cacccgacag agaattgcat caagaatgtg cacgagcgcg
     9361 gccgttcgaa ctaaggtcga aggatccatc tcgggtttta tgaaaaggag tccggagaga
     9421 taaccgaaca gatgatagaa atccaccacg aggccttgac gcttgctttt c
//
LOCUS       DASSWZ010000167         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_358982, whole genome shotgun sequence.
ACCESSION   DASSWZ010000167 DASSWZ010000000
VERSION     DASSWZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358982"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFN58_04160"
     CDS             <1..325
                     /locus_tag="VFN58_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917217.1"
                     /translation="SMAFAPCMPGKSPRLQHVMLEVESLDDVMRSYHFLRIHKAPIGM
                     GPGRHPNCQTVHVYVQTPGGFAVEFGWGHRRLDDATHQPIVYPPGSPVDVWGGDIQSP
                     EFELG"
     gene            complement(373..1296)
                     /locus_tag="VFN58_04165"
     CDS             complement(373..1296)
                     /locus_tag="VFN58_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917218.1"
                     /translation="MKKVPLPFSPIGINCLPWFVAKEARIYQKHGIDVDPVFIGASSA
                     LFQSMLSGAADMAGSGGPSVISNVLQGGDIIHVTAMVPRFTQSIMVKPEIKKPEDMIG
                     KKIGVSRLGTVTHFALQTALDGYGVKGVTILQMGGQPEAVAGLVRGSVDGAVFSPPYN
                     FQLKKQGYNELASPNDLQKFTAFITNGIVARRSVVEKDKDSLIRLIKSTSESIKLIAT
                     DREFTKKVITKWMPLKDPDLLEQVYRFATENYAKEGVVPEGALSAMVKQMVQSNLIDT
                     KMAANTPLTAYYDNRYVDEVKRSGFFDQLWK"
     gene            complement(1678..>2509)
                     /locus_tag="VFN58_04170"
     CDS             complement(1678..>2509)
                     /locus_tag="VFN58_04170"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HET8917219.1"
                     /translation="KPSQPNPSQPRRDAAPPPQQPSPFGSQSGGFMRTFGTAMLGGFL
                     GSMLFSGLANAGFGGVGGFGGSGFGLIEILLLAGLGYFLYRKFVRPAAATSYGSMQYQ
                     NTGYQIPSNTGYQAPTGAPVEEPPASNNDIDYRSLTMMDRSFDPNQFLKTAQDTFFKV
                     QGAWNKQDTAALRSLCGSELMQTWEQELNDLRARGHQNKMENIALRESEITEAWTENG
                     QDFITVRLHANLLDYTVDQKSGNVVSGNNSDPIEFQEYWTFTRPVGPNPWKLTAVQQA
                     "
BASE COUNT          549 a          668 c          700 g          592 t
ORIGIN      
        1 cagcatggct ttcgctccat gcatgccggg caaatctccc aggctgcaac acgtcatgct
       61 ggaagtggag tcactcgatg atgtgatgcg ctcgtatcat ttcctgcgca tccataaggc
      121 acccatcggg atggggcccg ggcggcatcc taattgtcag accgtacacg tctatgtgca
      181 aaccccggga ggatttgccg tggagtttgg ctggggccac cgccgtctcg acgacgccac
      241 gcatcagccg atcgtctatc ccccgggcag tcccgtcgat gtctggggag gcgacatcca
      301 gagccctgaa ttcgagctag ggtagcaccg gtactacttg atcaggggaa aagggaacgc
      361 cgaactgcgg cgctatttcc agagttgatc gaagaaaccg gaacgtttga cctcatcgac
      421 atagcggttg tcgtagtaag cggtcaacgg ggtattggcg gccattttcg tatcgatgag
      481 attgctttgc accatctgtt tgaccatggc actgagtgcg ccctccggta ccaccccctc
      541 tttggcatag ttttccgtcg caaaacgata gacctgttcc aataaatcag ggtcctttaa
      601 gggcatccat ttggtgatta ctttcttggt gaattctcgg tcggtggcga tgagcttaat
      661 ggattcagag gtgcttttga tcaaccgaat caaggagtct ttgtcctttt ccacgaccga
      721 tcggcgggca acaatgccgt tggtgataaa cgcggtaaac ttctgtaaat cattggggga
      781 cgcgagttcg ttgtatcctt gtttcttaag ttgaaagttg tacggtgggg aaaacacagc
      841 gccatcgaca gagccacgga ccaatcccgc taccgcttcg ggttgtccac ccatttgtag
      901 gatggtcacg cctttcactc cgtaaccgtc tagggccgtt tgcagcgcga aatgtgtgac
      961 ggtgccgagc cggctgacgc cgattttttt gccgatcatg tcttccggct ttttaatctc
     1021 cggcttaacc atgattgact gggtgaaccg cggaaccatg gcggtcacgt gaatgatgtc
     1081 gccgccttgc aaaacgttcg aaatgaccga cggtccgccg gacccggcca tatccgctgc
     1141 tcctgacagc atcgactgaa acagagccga ggatgccccg ataaaaaccg gatcgacgtc
     1201 gattccatgt ttttgataga tgcgagcttc cttggcgaca aaccagggca gacaattgat
     1261 ccctatgggt gaaaagggta gcggcacctt cttcatagcc tgcgccgaca acgacgaggg
     1321 accaaagaga agtaaaatca aacagacaaa cgcggtactc cgaaggctgc aacgattgga
     1381 ggcggaagag taaaggtcat gtgtggtttg cattaccgaa cctcctcttt ggtatgacgg
     1441 catggtctct atcgcaggcc tttcatgctg tcaatatcaa taaatgctac ataccgagca
     1501 agaccgcctt gtttcatgct ttgacagaaa gccgcccaca gttaaccgct tgaatggttt
     1561 gcaacgaagg actccccgtg acagcggcgc cgtgagccgc gccttttcca cgcctaaaag
     1621 cacaacagcg cacgatttcg tgcgctgttg tggctgaata ttcgttggcg ggccgtgcta
     1681 tgcctgttgc accgccgtta acttccacgg gttagggccc acgggacggg tgaaggtcca
     1741 gtattcttgg aactcaatcg gatcggagtt gttaccgctg accacgtttc cgcttttttg
     1801 atcgacggta taatcgagta gattggcgtg gaggcgaacg gtaataaagt cttggccgtt
     1861 ttccgtccag gcttcggtaa tctcgctttc ccgcagggcg atgttttcca ttttgttctg
     1921 gtgaccacga gcccgtaggt cgttcagctc ctgctcccag gtttgcatca actcggagcc
     1981 gcatagggaa cgcagagcgg ccgtatcctg cttgttccac gcgccctgaa ctttgaaaaa
     2041 agtatcctgc gcggttttca agaattgatt gggatcgaag ctccggtcca tcatcgtgag
     2101 ggaccgatag tcgatatcat tattgctcgc cggcggttct tcgaccgggg cgccggtggg
     2161 agcttgatag ccggtgttac tggggatttg atatccggta ttctgatact gcattgaccc
     2221 gtagctcgta gcggccgcgg gtctaacaaa cttgcgatag agaaaatatc ccaggccggc
     2281 caacagaagg atctcgataa gtccgaaacc gctaccgccg aacccgccca cgccgccaaa
     2341 gcccgcattc gctaatcctg agaagagcat ggagcctaga aatccgccca acatggcggt
     2401 gccaaaggta cgcataaagc cgcccgactg cgacccgaac gggctaggct gctggggagg
     2461 tggagctgcg tctcttctag gttgcgaggg atttggttgc gagggcttg
//
LOCUS       DASSWZ010000168         9030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_783708, whole genome shotgun sequence.
ACCESSION   DASSWZ010000168 DASSWZ010000000
VERSION     DASSWZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783708"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFN58_04175"
     CDS             <1..801
                     /locus_tag="VFN58_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917220.1"
                     /translation="RVLSEYQAKRYDVDIVSGSAATSMVQRAGFMQRFYSPHLAEYPP
                     ELKDAKGFWGSTNIYFMTLGYNTRNVKANELPKSYEDLLHPRWKGQMMWSTSRGSGAP
                     QFIGNILLTMGQEAGKAYLQKLKSQNIAKSTASARQILDLVIAGEYPLAVQIFNHHAY
                     ISKAAGAPVEWQPLEPVTATINTIGLTKNAPRPHAAMLFLDFLLSKKGQKIVQVSNYL
                     PAHPEMPALQADLKPGGGRFKKANYINPDVLYDQGNEWVDYFQNQFLK"
     gene            889..2598
                     /locus_tag="VFN58_04180"
     CDS             889..2598
                     /locus_tag="VFN58_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8917221.1"
                     /translation="MFRLEVLSRFEAQTWVLVVVSLVTAYLVLPPLYSVIQTSLFTTK
                     LTGELDEFTLSYYRDMFRELQVLGPFLNTVYFSVGSALLATLLGGLVAWIVTRTDSPL
                     RGLGYFTAFASFGTPFILYTIGWLLILGKAGPVNSWLRVFLDHGGPVVNVYSMWGMVF
                     VEALLWSPFVFLLLAASFRSMDPSLEEASAVCGARVWQTLRRISLRLMLPAFFSVLLL
                     IFIRAFESFEIPALVGLPGDIRVLTTSILLDAQRMPPTYGRAGAFSVLLMLVVACTLY
                     FYFRMTREASRFHTITGKGYRPALIQLGGWRYVTAAGLLLYSFVLLVLPFLIVLWASL
                     LPFYIQPSLEAVSKFTLKNYVTAIHFPKITDSIKNSVLLGLGSATAVMVLTTLASWIL
                     VRTKLRGRWLLDLLTTLPLLFPGIVMGLAIMRFYLLVPVPIYGTLWILLVAFVTRYIP
                     YGIRYTHSGLLQLHRELEEAAYASGASWLNAMRRITMPLLTPSFLGGWVFIFLLSAKE
                     LSMSILLVSPQTPVVSVAIYELWENGQVGELAAFGVIWTTILVSIAVAYYLFARRYGI
                     QQH"
     gene            2826..3380
                     /locus_tag="VFN58_04185"
     CDS             2826..3380
                     /locus_tag="VFN58_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002208541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET8917222.1"
                     /translation="MPTIFSHPAVPLALGFGLGREIISRRLLLAGVAASILPDLDVVA
                     FSFGIPYEHEFGYRGFSHSFFFAALVALLGACARRRLNTSFGIAFGFLFLATASHGVL
                     DALTNGGLGIAFFWPWSHERYFLRYQVIEVSPIGPSHFFSRWGIKVILSELRWVWLPC
                     ALMGLGLAVIKKTTLDTYLRGIIR"
     gene            3481..4836
                     /locus_tag="VFN58_04190"
     CDS             3481..4836
                     /locus_tag="VFN58_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET8917223.1"
                     /translation="MSPSTREKKGSIEQLKTKVFDLIWDPEISEVTRFKRLIHSLLKI
                     GFMVIRDFIENLVNLEAMALAFKTLLSLAPLLAVIFSILKGFGVHNRMEPALAEALAP
                     LGEKGKEVTGYLIGFVDKMSAGALGSIGLVTLFITVLSLMGTIEQAFNRIWHVKSSRK
                     LTRRFSDYLSVILVGPVLVVAALTITATLQSNAFVQKMISLEPFGTVILTFLKFVPYF
                     TLWGAFTFLYFLVPNTRVKLTSALVGGLVAAVLWQTVGWGFTVFVASSTRYYAIYSSF
                     ALLLLFLLWLHVGWVIVLLGAQVAYAHQHFRFYEGERELLVNSPAGREKLALHMMFLI
                     GRNFFHGLDPLNVTALANQLYLPAGLVRDFMETFRQSRLVLPVADEETFVLARDPERI
                     SIKEILDCVRNSGVRKQTVRTDEEVFIEAVLREVDDSVSQALEGKSLQSLIVEHSRPS
                     A"
     gene            complement(5102..6625)
                     /locus_tag="VFN58_04195"
     CDS             complement(5102..6625)
                     /locus_tag="VFN58_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET8917224.1"
                     /translation="MKKLIWISGTILIGLVAAVLVVPHFIDLGIFKRSYLPLVEEILR
                     RRIDVGEVRLSLVPTPSIRLSNLKVSDSPAFPSNDSFAAERLQLRLKFWPLLQGRFEV
                     TEFVLEKPVINLRKGPDGTFNYPDLADKKGSLDRARESRKRASASKPQESAVMPLIVP
                     SRMRVRDGQLNFQTLGQKSFTINGIDLSLQEFSVDRPFPYRASFSLPGLKLIALEGML
                     DYQEGQSTLRLNETRLKVQDLVLPVEGNISNLSTVPSLNLRLGADRLDAKPIFQILSA
                     FALAPSDTEVSGPMGLRITMTGPSHSLVTQVRGQLKDVKVQNKRVLKGTLSGEVFIKL
                     PLSGGGTAARRLQGNGNLVARNGELTNVDLINKVQRVTGLIGLSPNERREATTFKVLE
                     TAFVVADGFADFKQIYLINPQIEMNGSGTMTLERPSLNIAMEAVLSAQASARAGKGKT
                     ATFFKDSQGRIVVPLKITGPVESASVDLDRSKMLQRGSGQVVEKGFGIFFKQLFRQR"
     gene            6818..7786
                     /locus_tag="VFN58_04200"
     CDS             6818..7786
                     /locus_tag="VFN58_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8917225.1"
                     /translation="MELALDILPQVLVDGIVLGFMYALIALGYTMVYGVLEFINFAHS
                     EIFVLGAFVGVEVLLLLESAGHLATLHTAFVLLLVILSGMLLSGLAAVALERVAYRPL
                     QGSPRLVALISAIGASFFLQDALRFFESLWRNTFYLTYPTLDVLDQTVPLGLNVAVPV
                     KSLLVIVTALVSLAALHLFVNRTRLGTAIRAVAQDPDTASLMGIPVNRVIALTFFVGG
                     AMGGLAGVLFGLHYSLVNPYSGFFPGLKAFTAAVLGGIGNIPGAMLGGMVLGMLEAFA
                     ASYLSILTDGAFGAEYKDVFAFVVLILILILRPKGLLGEKVREEGA"
     gene            7783..9030
                     /locus_tag="VFN58_04205"
     CDS             7783..9030
                     /locus_tag="VFN58_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET8917226.1"
                     /translation="MKVGLVHLGVVAYIIVSAALVFAFSQSVLAFLFFEASLLVIYYA
                     SVDRTTKYFLGAIVLLVLLPLLGSVNGYFLEVAIQAGIFIALALGLNIVVGFVGLLNL
                     GFVAFYAIGAYLWAIFGSPQANHFVPGNLFPLSSVWFFPFLLLSVGVGALTGALLGLP
                     VLRLRGDYLAVVTLGFAEVVRALVNNFDKPINITNGPKGITPIEKPPLFFQPLLEFLG
                     FSVNPSTLYPFYFYFLVLSIVMLTVLVARRLEESRIGRAWKAIREDEIAAAAMGIPVV
                     RMKLLGLAVGASFASAVGVLFASKQVFINPESFTFMESIGVLAMVILGGMGSIPGAIL
                     GATGVTILNLQVLKGLSLWLNSVRQSGIVLSIPLFGDFTLSQIPAQFEPAKYERMVFG
                     LILILMMMFRPQGILPPRQKRGH"
BASE COUNT         1708 a         2398 c         2532 g         2392 t
ORIGIN      
        1 agggtgctca gcgagtacca ggcgaagaga tatgacgtcg atatcgtcag cggcagcgct
       61 gcaaccagca tggtgcagcg ggcaggattc atgcagcgat tttattcgcc tcatctggcc
      121 gagtatccgc cagagctcaa agatgcaaaa ggtttctggg gtagcaccaa catctatttc
      181 atgaccctcg gttacaatac ccgtaacgtt aaggcgaacg aattgccaaa gagctatgag
      241 gatctgcttc atccgcgttg gaaaggccag atgatgtggt caaccagccg tggttccggt
      301 gccccccagt tcatcggtaa cattctcctc acgatgggac aggaagcagg caaggcctac
      361 ctgcagaagc tcaagagtca aaatattgcc aaaagcaccg ccagcgccag acagattctc
      421 gatctggtga tcgcgggaga atatccgctg gcggtgcaga tctttaatca tcatgcctat
      481 atcagcaaag ctgccggagc gccggtggag tggcagcccc tggaaccggt tacggcgact
      541 ataaacacga tcggcttgac gaagaatgcg ccgcggccgc acgcggctat gttgttcctc
      601 gactttctgt tatccaaaaa agggcagaag atcgtccagg tatcgaatta tctccccgcg
      661 catccggaga tgcccgctct acaggcggac ctcaagcccg gcggcggcag attcaaaaag
      721 gccaattaca tcaatccgga tgtcctctac gatcagggca acgaatgggt ggactatttc
      781 cagaaccagt tcctgaaata gaaaattgcg actcccgtgg ccaaacaaca gtcatcggaa
      841 acttttaagc taccactagg gtccggcaaa acgcggcaag cgcgcggtgt gttccggcta
      901 gaagtccttt cccgtttcga agcgcagacc tgggtgctgg tcgtggtctc gctcgtgacg
      961 gcgtatctcg tcctgcctcc cctctattca gttatccaga ctagtctttt caccaccaaa
     1021 ctcaccgggg aactggatga attcaccttg agctactatc gggacatgtt tcgcgaacta
     1081 caggtgctcg ggccgttttt gaacacggtc tatttctccg tcggcagcgc gctgctggca
     1141 acgctcttgg gcggcctggt tgcctggatc gtaacacgca cggactcgcc actgcgcggt
     1201 ttgggatatt tcaccgcttt tgcttccttc ggcacgccat ttattttgta caccatcggc
     1261 tggctcctta ttctaggcaa agccggaccg gtgaacagtt ggctgcgcgt atttctcgat
     1321 cacggcggtc cggtggtgaa tgtctattcg atgtggggga tggttttcgt cgaagcgctg
     1381 ctctggtcgc ccttcgtgtt tcttctgctc gcggcgtcgt ttcgttccat ggacccgtct
     1441 ttggaagaag cgtccgccgt ctgcggcgcg cgggtttggc agaccctcag gcggatttcg
     1501 ctgcggctca tgctgccggc atttttctct gttttgctgc tgattttcat tcgcgccttc
     1561 gaatcatttg aaattccggc cctggtcggg ctgccggggg atattcgcgt tctgacgacg
     1621 tcgattcttc tcgatgccca acggatgccg ccgacctacg gcagggccgg ggcgttttct
     1681 gtgctcttga tgttggtggt agcgtgcacg ctctatttct atttccgaat gacgcgggaa
     1741 gcctcacgat tccataccat caccggcaag ggttatcggc ctgccttgat ccagctcggt
     1801 ggttggcgct atgtgacggc ggcgggcttg ttgctctact cctttgtgct tctggtgctt
     1861 ccgtttctca tcgtcctctg ggcctcgctc ctcccgttct atattcagcc ctcgttggaa
     1921 gcggtatcca agttcacctt gaagaactac gtgacggcga tccacttccc gaaaatcacc
     1981 gactccatca agaacagcgt cttgctcggt ctcggcagcg ccaccgcggt gatggtgctg
     2041 acgacgttgg cttcgtggat cttagtacgc accaagttgc gcggccgctg gctgctcgat
     2101 ctgttgacga ccctgccact cctgtttccc ggcatcgtga tgggcttggc gatcatgcga
     2161 ttttatctgc tcgtgccggt tcccatctac ggtacgctat ggattttgct ggtagcgttt
     2221 gtcacgcgtt acattcccta cggcattcgt tacacgcatt ccggcctgtt acagctgcac
     2281 cgggaattgg aagaagcggc gtatgcttcc ggggcttcgt ggttgaatgc gatgcggcgg
     2341 atcacgatgc ccttgctgac cccgtctttc ttgggcggat gggtttttat tttccttctc
     2401 tcggccaagg agctgtccat gtcgatcctg ctcgtcagtc cgcaaacgcc ggtggtttcc
     2461 gtcgctatct atgagttgtg ggaaaacggt caggtcggcg agctagccgc cttcggcgtg
     2521 atctggacga cgattcttgt ttccatcgcc gtcgcctact atctcttcgc gcgccgctac
     2581 ggcatacagc agcattgagc ctttgagaaa gtatgggttc ttccgccctg ccccagcgtt
     2641 tcatgacacg gggtcttacg gttgcaaatt gttgcaagca gaggtgaccc atcgattttt
     2701 gtggggaaag gatccaggta atgaatgggc gaaccccacc ggtcactcct tttatcattc
     2761 ggcgttgcgc gagcgtgtca cgggtcaggt ggaagtggat tgaaagtttt gctgcttagt
     2821 ttctgatgcc gacgattttt tcgcatccgg ccgtaccgct tgctttaggc ttcggtctgg
     2881 gcagggagat catttcaaga cgactccttt tggctggggt tgcggcatcg atcttgccgg
     2941 atctcgacgt ggttgccttt agtttcggta ttccttacga acacgagttt gggtatcgcg
     3001 ggttcagcca ctcatttttt ttcgctgctc tcgtggctct actcggcgcg tgcgcgcggc
     3061 gccggttaaa taccagtttc ggcatcgctt tcgggttctt atttcttgcc acagcctctc
     3121 acggcgtgct ggacgcgctt accaacggtg gccttggtat cgcatttttc tggccctggt
     3181 cccatgagcg gtacttcttg cgatatcagg tgatcgaagt ctcgcccatt ggcccttcac
     3241 actttttctc ccggtgggga attaaggtaa ttctttccga gctgcgttgg gtttggctgc
     3301 cctgcgcgtt aatgggactt gggctggcag ttatcaaaaa aacaaccctt gacacttatc
     3361 tacggggaat catccgttga gtttgatctt tagacctgta cccaaagttg gtagagttta
     3421 atatactcgt tttcaacccg tcgtttgctg atttgagtct cttacaagcc cacgaccgtc
     3481 atgagcccat cgacccgaga gaaaaaagga tccattgagc agctcaagac taaagtcttc
     3541 gatctgattt gggatcccga aatttctgag gtgacccgct tcaaacggct gattcactcg
     3601 ctgctcaaga tcggtttcat ggtcattcgg gacttcatcg agaatttggt gaacctagag
     3661 gccatggcat tggcattcaa gacactgcta tctctcgcac cgctgttggc tgtgattttt
     3721 tccattctca agggcttcgg cgtgcacaat cgaatggagc cggctcttgc cgaggcgctc
     3781 gccccccttg gcgagaaagg caaagaggtt acgggttatc tcattggctt cgtcgacaag
     3841 atgagtgcgg gggcgctggg cagcattgga ttggtgacct tgtttatcac agtactgtct
     3901 ctgatgggga cgatcgaaca ggctttcaat cgcatctggc acgttaagtc gtcgcggaag
     3961 ctgactcgca ggttcagcga ctatctaagc gtgattctcg tcggtccggt gttggtcgta
     4021 gcggcgctga cgatcaccgc caccttgcaa agcaacgcct ttgtccaaaa aatgatttcc
     4081 ctcgagccgt tcggaaccgt gatcttaacg ttcctcaaat tcgtgcctta ttttaccctc
     4141 tggggggcat tcaccttcct ctatttctta gttccaaaca cccgcgtgaa gcttacatcc
     4201 gcactggtgg gcggcttggt ggcggcggtg ctttggcaaa ccgtgggttg gggatttact
     4261 gtttttgttg catcctcgac tcgctactac gcaatttact ccagcttcgc gttacttctt
     4321 ttatttctgc tttggctgca cgtcggttgg gtgatcgtgc tgctgggggc gcaggtagcc
     4381 tatgcccatc aacactttcg tttctatgag ggagagcggg agcttttggt caacagtccg
     4441 gcaggacgtg aaaaattggc gcttcacatg atgttcttaa tcgggcgcaa tttcttccat
     4501 ggccttgatc cgttgaacgt gacggcgctg gcaaaccaac tctatctgcc ggcaggtttg
     4561 gtgagggatt tcatggagac gtttcgtcag agtcgtttgg tgttgcccgt agcggatgaa
     4621 gaaaccttcg ttctggcccg cgacccggaa aggatcagca tcaaagaaat cctcgactgt
     4681 gtgagaaatt ccggcgtgag aaaacagact gtccgaactg atgaagaagt tttcatcgaa
     4741 gcagtgctgc gggaagtcga cgactcggtg tcgcaagcgc tagagggaaa gagtctgcaa
     4801 agtctcatcg tcgaacatag ccggccctcc gcttgatcac gggcttgcag ggagcgagcc
     4861 ggattattca ctggcaagcg acgatcaagg ggcgagctct ccggatcgaa ggcaataagc
     4921 ctctatcttg aggctgcctg caaacctaag ttctgaagcg gatacctggg aacaattcga
     4981 gcgcacggcc aagcattata aacgtcattg cagctgtttg tctctatgcc ttcgctcctc
     5041 caagctcatc ccttgatcga cactgtgtca tggtttttcc tcggagcttg aacagtccgg
     5101 gctagcgctg gcggaaaagc tgcttgaaga agatgccaaa gcctttttcc acgacttgtc
     5161 cagaaccgcg ttgaagcatt ttcgacctgt cgaggtccac cgacgcgctc tcaaccggcc
     5221 cggtgatctt aagcggcacg acgatccgcc cctggctgtc tttgaaaaaa gtggcagttt
     5281 ttcccttgcc ggcgcgggcc gacgcctgag ctgaaagcac agcctccatc gcgatgttca
     5341 aactgggtcg ctcgagggtc atggtaccgc tgccgttcat ttcaatttgc gggttgatca
     5401 agtagatctg tttgaaatcg gcaaagccat cggccacgac gaatgcggtt tccaggactt
     5461 tgaaagtcgt tgcctctcgc cgctcgtttg gtgatagacc gataagacct gttacccgct
     5521 gcactttgtt gatcagatcc acgttggtca gttcgccgtt ccgagccact agatttccat
     5581 tgccttgcaa acgtcgcgcg gcggtccctc cgccgctcag tggcagcttg atgaaaacct
     5641 cgccgcttag ggttcccttc aagactcgtt tgttctgcac tttgacgtcc ttcaactgtc
     5701 cgcgcacttg ggtgaccaga ctgtgcgacg ggccggtcat ggtgatgcgc aaccccatcg
     5761 gtcccgagac ttccgtatcg ctcggcgcta gagcgaaggc ggagagaatc tggaagatgg
     5821 gcttggcatc aaggcgatcg gcgcccagcc tcaagttgag gctgggcaca gtcgaaaggt
     5881 tgctaatgtt cccttctacg ggaagaacga gatcttgaac cttcagacgc gtttcattca
     5941 gccgcaaggt cgattggcct tcttgataat cgagcatgcc ctccaacgcg atgagcttca
     6001 agccgggaag gctgaaagac gcgcgatagg gaaatggacg atcgacggag aattcttgaa
     6061 gcgacagatc gatgccgtta atagtaaaac tcttctgacc cagtgtctga aagttcagct
     6121 ggccgtcccg gacacgcatc cggctgggca caatcagcgg catgacggca gattcctggg
     6181 gtttggaggc agaggctctc ttcctgctct ctcgtgctct gtcgagagag cctttcttgt
     6241 ccgcgaggtc aggataattg aaagttccat caggcccttt gcgtaagtta atgacaggtt
     6301 tttcgagaac gaattccgtt acctcaaaac ggccctgcag cagcggccaa aacttgagcc
     6361 ggagttgcaa tcgctccgcg gcaaaagagt cattgctggg aaaagccggg ctatcagaga
     6421 ctttcagatt cgatagacga atcgaaggag tggggacgag gctaaggcgc acttcgccaa
     6481 cgtcgatgcg ccggcggagg atttcttcta cgagcggaag ataacttcgc ttgaagatgc
     6541 ccagatcgat gaagtgcggt accacgagga ccgcggccac caagcctatc aagatggtac
     6601 cgctgatcca gattagcttt ttcattccag cgtctctggg gagctattga gcggtaaggg
     6661 tatgttagcc ttcgtgcgat aggatgggtc aatgcggggg aatatacgcg attgaagcat
     6721 ggtttcaacc ggaacgattc cgacttgccg gatttatacg gtggcgggca agaagaggat
     6781 ttgttagcat acccactttt gaaaggatca gacgagtttg gaactcgcgc tcgatatttt
     6841 accacaagta ctcgtggatg gcatcgtact gggcttcatg tacgccctga tcgccctggg
     6901 ctacaccatg gtttacgggg tcttggagtt tatcaatttt gcccacagcg agatcttcgt
     6961 tcttggcgcc tttgtcggcg tggaggtcct cctgctgctg gaaagcgccg gccatttggc
     7021 aacgcttcac accgctttcg tcttgctgct cgtgattctc agcggcatgc tgctcagcgg
     7081 tctggcggcc gtggcgttag agcgagtggc ttatcgtccg ctccagggaa gcccacgact
     7141 cgttgcgttg atctccgcta tcggcgcttc attttttctg caggacgctc tccgtttttt
     7201 cgaaagtctt tggcggaata ccttttacct cacctatccc acactggatg tcctcgatca
     7261 gaccgtgccg ctggggctca atgtggcggt gccggtgaaa tcgttgctgg tgattgtgac
     7321 cgcgttggtg agcttggcag cgctccatct ttttgtcaat cggacgagac tgggcacggc
     7381 gattcgggcg gtggctcaag atccggatac tgcgtcccta atgggtatcc cggtgaaccg
     7441 ggtgattgca ctaacttttt ttgttggcgg ggcgatggga ggtttggccg gcgtgctttt
     7501 tggtttgcat tacagcttgg tcaaccccta cagcggattt tttcccgggc tcaaggcctt
     7561 taccgctgcc gtgcttggcg gcatcggaaa tattccgggc gctatgttgg gcggaatggt
     7621 gctaggcatg ttggaagcgt ttgcggcatc ctatctctcg attctcaccg acggagcatt
     7681 cggcgcggag tataaagatg tctttgcttt cgttgtcctc atcctgattc tcatccttcg
     7741 cccgaaggga ctcttggggg agaaggtgcg ggaggaagga gcttgaaagt tggcttggtc
     7801 catctgggag tcgtcgcata tatcatcgtg agcgccgctc tcgtcttcgc cttttctcag
     7861 tcggtcctgg cttttctgtt ctttgaagcc tccctcctgg tcatttacta tgcgtcggtc
     7921 gatcggacca cgaagtattt tttgggcgcc atagtgttgc tcgtgttgtt gccgctgctg
     7981 gggtccgtca acggctactt tctggaagtt gctattcaag ccggcatttt tatcgcgctg
     8041 gcgctgggac tcaacatagt ggtcggcttc gtcggactgc tcaatttggg tttcgttgcg
     8101 ttctatgcca tcggggcgta tctctgggcg atttttggct cgccccaggc gaatcacttt
     8161 gtgcccggca atttgtttcc cctttcgtcg gtttggtttt ttccgtttct cttgctgagc
     8221 gtcggagtgg gcgctttaac aggggcgtta ctgggcctcc cggttctccg gctgcgcggc
     8281 gactatctgg ccgttgttac cttgggtttt gccgaggttg tgcgcgctct ggtgaacaac
     8341 ttcgataaac cgatcaacat caccaacggt ccgaagggca tcacgccgat tgagaagcca
     8401 ccgctttttt ttcagccact gctagaattt ctcgggttta gtgtgaatcc gtcgacgctc
     8461 tatccattct acttctattt cctcgtctta tctatcgtga tgctcacagt tctggtcgcg
     8521 cgaagacttg aagagtcacg gatcgggcgg gcatggaagg cgattcgcga ggacgaaatc
     8581 gcggcggccg ccatgggaat tccggtcgta cggatgaagt tgcttggcct tgccgtggga
     8641 gcctcttttg cctcggcggt cggtgtgctg tttgcgtcca agcaagtatt catcaatccg
     8701 gagagcttta ccttcatgga atccatcggg gttctcgcca tggtgatcct cggtggcatg
     8761 ggctcgatcc ccggcgcgat tctcggcgcc accggcgtga cgattttgaa tctgcaagtt
     8821 ctcaaagggc tttccttgtg gctaaactcc gtccgccagt cgggaatcgt gttgtctatt
     8881 ccgctcttcg gcgattttac tttgagtcag attcccgccc agtttgagcc ggcgaaatat
     8941 gagcggatgg tttttggttt gatcctgatt ctcatgatga tgtttcggcc gcagggcatc
     9001 ttgccgcctc ggcagaagcg gggacattga
//
LOCUS       DASSWZ010000169         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1024217, whole genome shotgun sequence.
ACCESSION   DASSWZ010000169 DASSWZ010000000
VERSION     DASSWZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024217"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFN58_04210"
     CDS             <1..499
                     /locus_tag="VFN58_04210"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8917227.1"
                     /translation="AIMFTDLKDSTLMTTLYGDTKALHLLHVHNALTRNALKSHRGRE
                     VKHTGDGIMASFAAVPDAVGCAITIQKAFEAYNKETPEAPLYLRIGLTAGEPIEEHGD
                     LFGKAVQLAARLCAHAEAGRILVDQVMLDQWQGKELPFSDLGEVTPKGFDHAVRVYEV
                     NWTAA"
     gene            550..1980
                     /locus_tag="VFN58_04215"
     CDS             550..1980
                     /locus_tag="VFN58_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET8917228.1"
                     /translation="MSGLKVRTRANGEITLQEDTIRKFKESLRGELILSDDAGYDDAR
                     SIWNAMIDRRPALIARCLGVTDVVTCVNFAREHGLILSIKGGGHNISGLAVCEGGLML
                     DMSRMRGVWVDPSTRTARAQAGCLLGDVDRETQLHGLAAVLGFISNTGIAGLTLGGGF
                     GYLTRQFGWTSDNLLSMEVVTEDGRIVRASDKENRDLFWGLCGGGGNFGVVTGFEYKL
                     YPVGPEVMAGAIAWRAEYAGEVLEMLRTLMEQAPPELVCVAGLRIAPPAPWLSKDIHG
                     KRIVALFVCCTAETSEGEKLVAPIKAFGSTVGDIIQRRPYVSQQSLLDATQPKGRRYY
                     WKSEYLPKLQPELLAKTIRHAEAIASPHSAILLFPLDGALNRLSEDHSSVGNRDTGWV
                     LNIAAAWDKPEDDKTNIDWARTAWRDMRRFSTGGTYVNFLTEEEGDERIHAAYGNNHE
                     RLVEIKTKWDPANLFRVNKNIAPSRT"
     gene            complement(2476..4047)
                     /locus_tag="VFN58_04220"
     CDS             complement(2476..4047)
                     /locus_tag="VFN58_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8917229.1"
                     /translation="MSKSRVAIVGSGIVGTAMAYVLTKNGYPVDIFEKGPQYPYPHTK
                     QFTERIRFLYKNPVYSLSEDLNQVMISGDYRWDPEEERHLVVGGSATQWNAITLRMGP
                     NDFKTKSLYGYGADWPLSYADLEPYYCRAEAYLGVSGTDADNPFAPQRSKPFPLPPFE
                     LSYDDKIMAERLHKHGIVLHTTPQARTRTDYEERPGCQNFGVCHVCPIGVRYSPNYHL
                     LRALGTGLCQVHENASVRRIAIDKSGRAQALVYRSTDATKEREHAADVIVVAAGAIES
                     VRLLLLSADSGHPAGLGNDRGQVGKHFTFHHLWSGAYHYPSPLYPERFGGPTGQSHQF
                     LNHPSRGSRGAIKIEFGSRSGSDLVNQGKVTKWDGVSEIIEQLKPRLHWRLIIFHNES
                     VPSPEKYIGLSEERDRFGDPYANVHYSASEFDHETYLFALKLFDSFAEATGSDEKVMS
                     PVFWSGGHHMGGCRMGQMVGESVVDSFGKLHGSPNLFVIGGSNFVGTSGAVNPTLTMV
                     ALALRTADFIGDQFS"
BASE COUNT         1124 a         1350 c         1287 g         1049 t
ORIGIN      
        1 cgccatcatg ttcacggatt tgaaagattc gaccttgatg accacactgt atggagacac
       61 caaggcttta catctgcttc atgtccataa tgcgttaaca cgcaatgcgc tcaagtcgca
      121 ccggggacgt gaggtcaagc acacgggtga cggaattatg gcgtcctttg ccgcggtacc
      181 ggatgcggtc gggtgcgcca ttaccatcca gaaggcgttc gaagcgtata acaaagaaac
      241 cccggaagcg ccgctctatt tacgcattgg tctgaccgcc ggcgaaccca ttgaggaaca
      301 tggcgacctg tttggcaagg cggtccaact ggcggcgcgg ctttgtgcgc atgctgaagc
      361 aggccggatt ctagttgatc aggtgatgct ggaccaatgg caaggcaaag agttgccgtt
      421 ttcagattta ggcgaagtaa cacccaaagg tttcgatcac gccgtccgcg tgtacgaagt
      481 caactggacg gcggcctaaa ctctacaatg cgtacgccaa tacgtttctc gttagggaag
      541 gagctcggta tgtctggcct gaaagttaga acccgcgcga atggggaaat cactctgcaa
      601 gaagacacca tccggaaatt caaggagagc ctgcgtggtg agctgattct atcggacgac
      661 gctgggtacg atgatgcgcg ctccatctgg aacgccatga tcgaccgccg gcccgcgctc
      721 attgcgcgct gcctcggcgt taccgatgtt gtaacctgcg ttaacttcgc gcgcgagcac
      781 ggtctcatac tctccatcaa aggcggcggt cacaacattt caggacttgc cgtgtgcgag
      841 ggaggattga tgctcgacat gtcgcgcatg cgcggcgtgt gggtggaccc gagcacacgc
      901 acggcccgcg cccaagctgg ctgtctcctg ggcgacgtgg accgcgagac tcaactccat
      961 ggccttgctg cggtgctcgg ctttatctct aacacaggca ttgccggcct cacccttggc
     1021 ggcgggttcg gttacctcac gcgccagttt ggctggacca gcgacaacct cctgtcgatg
     1081 gaggtggtca ccgaggacgg acgaatcgta cgcgcaagcg ataaggaaaa tcgcgatctg
     1141 ttctggggcc tttgcggagg gggcggcaac ttcggcgtcg tgaccggctt cgagtataag
     1201 ctgtaccccg taggcccgga ggtgatggca ggagcgatcg cctggcgcgc cgagtacgct
     1261 ggcgaggtcc tcgaaatgct ccgcaccctc atggaacaag ctcctccgga gctcgtttgc
     1321 gtagccggtc tccgcatcgc cccaccagca ccgtggttgt ctaaggacat ccacggcaaa
     1381 cgcattgtcg cgctcttcgt ctgctgcacc gcggagacaa gcgagggtga aaaactggtc
     1441 gctccaatca aggccttcgg gtctactgtg ggcgacatta ttcagcggcg accctatgtt
     1501 tcacagcaaa gcctcctcga cgccacccag ccgaaaggtc ggcgctacta ttggaagtcc
     1561 gaatatttgc ccaaacttca gcctgaacta ctcgctaaga cgattcgtca tgcggaagcc
     1621 atcgcctctc cccactcagc gatcttgctc tttccgcttg acggagcact caaccggctc
     1681 tcggaggatc actcctccgt gggcaaccgg gatactggct gggtcctaaa catcgcagcc
     1741 gcgtgggaca aacctgagga cgacaaaacg aacatcgatt gggcacgcac tgcatggcgg
     1801 gacatgcgcc gcttctccac gggcggcaca tacgtcaact tcctgacaga agaagagggc
     1861 gacgagcgca tccatgctgc ttacggaaac aaccacgagc ggctcgttga gatcaaaaca
     1921 aaatgggatc cggcgaatct gtttcgggtg aacaagaaca tcgcgccgag taggacatga
     1981 agtttaggat cttcttgaca gcccttcggc atgtgtattt gtgcgctcgt aacaagtctg
     2041 aacgcaacaa tccaaccttc cgctattcca atctttttcc gtgtcaaaac ggaacgtcgt
     2101 gctaccgtgg cggtagaggc tcagggccgg ccagcttgtt aaacagctca acgacgtcgg
     2161 catcgcgggg catttctttg atggctttct cgaacgttaa ttcttgtaga attcgacggg
     2221 atgccagtct cgctccttga acaaccaaat tccgcggtcc aggtgagctt cgatctgccc
     2281 ttcaacttgc aaaaatttga catcgttgtc gctgttcccg gaacttcacg cacggatgat
     2341 aatcggcagc agagtaaatg atcggcatcg ccgagtgact agccgggatt tttcgttggt
     2401 ggaaaaccac gacacagtgt cgatcgagag acgagctagg agaagcgaag gcacagagac
     2461 ggcgaataat ccgagctagg agaactgatc gccaataaag tctgccgtcc gaagtgccag
     2521 tgctaccatg gtcagcgtag gattgaccgc ccccgaagta ccgacaaagt tgctaccccc
     2581 aataacaaac aggttgggac taccgtggag cttgccaaac gagtctacaa cactctcccc
     2641 aaccatctgt cccatacgac acccacccat gtggtgaccc ccagaccaaa aaacagggga
     2701 catgaccttt tcgtcactgc ccgttgcttc tgcaaaggaa tcgaataatt ttagcgcaaa
     2761 tagataggtc tcgtggtcaa actcagaggc gctatagtga acgttcgcat aggggtctcc
     2821 gaaccggtcc cgctcctcag ataatccgat gtatttttcc gggctcggaa cggactcgtt
     2881 atgaaagata atgagacgcc agtgaagtct tggtttcaat tgttctatga tttcggagac
     2941 cccgtcccac ttcgtcacct tcccctgatt aactaagtca gatccgctac gggaaccaaa
     3001 ttcgatcttg atcgcaccgc ggctgcctcg gcttgggtgg ttgaggaact gatgactctg
     3061 tccggtaggc ccgccaaatc gttccggata caacggcgat gggtagtggt acgctccgga
     3121 ccaaagatgg tggaacgtga agtgttttcc gacctggcct ctgtcattcc ccaacccggc
     3181 aggatgaccg ctgtctgccg aaagaagcaa caaacggaca gactcgatgg ccccagcagc
     3241 cacaacaatc acgtctgctg cgtgttctcg ttccttcgtg gcgtcagtgg atcggtagac
     3301 caaagcttga gctctcccgg acttgtctat cgcaattctt cgcacggagg cgttttcgtg
     3361 tacttgacag agacctgtcc cgagagcgcg caggagatga taattagggg aatagcgaac
     3421 cccgatcgga cagacatgac aaaccccaaa gttctgacag ccaggtcgct cttcgtagtc
     3481 agtccgtgtt ctcgcctgtg gcgtcgtgtg gagaacgata ccgtgcttgt ggagtctttc
     3541 ggccatgatt ttatcgtcat agctgagctc aaacggcggt aaaggaaagg gtttggaccg
     3601 ctgtggagca aacggattat cagcgtcggt gcctgaaacc cccaggtatg cttcggcccg
     3661 acaatagtac ggttccaagt cagcgtagct aagcggccaa tccgcaccgt aaccgtaaag
     3721 acttttcgtc ttgaagtcat tgggacccat tctaagggta atggcattcc actgagtcgc
     3781 agagccccca acgaccaagt gacgttcctc ctctggatcc catcggtagt cacccgagat
     3841 catcacttga ttcagatctt cggaaagact gtaaaccggg ttcttgtaca ggaaacgtat
     3901 tctctcggta aattgcttgg tgtgtgggta gggatattgg ggacctttct cgaagatgtc
     3961 cacaggatat ccattcttag tcaagacgta cgccatcgct gtcccgacaa ttcctgaccc
     4021 aacgatggcg actctgcttt tgctcatttg caggctagcc taacagccta cgacggggcg
     4081 aacactgcca cgacgcagac cgccgactcc cttgcgaagc cttctgtgat acgggtgaaa
     4141 tctttctcat tatgttttac cgggtgcttt ggtatacgaa tcaagacctc tgggagtacc
     4201 aggccaagac tcatagccta acaaaatcca cgcgtcggtc cgtgaaaaca acgcacggat
     4261 ctgctcgaag atatacttct caaagcgcaa accatcgcgt tcgaaaactc gcgcgtatgg
     4321 gctagctgtc atccttcgca gtatcttccg tcgcacctcg atgtcagagc tggcaaaatc
     4381 agatttctgt gcctcctttg ctgcgcggtc aacaactagc acgaatcgct cataaagact
     4441 tcggtatcca ctaagattct cggaccgcca gcgaaaaaac gcttcataat gacttgtctc
     4501 gacctcggta ccaacgagcg tctcggtcgt cgccagcagg gctcggaccg tcccctcatc
     4561 cagtagaccg ttgggtgcat tttgaagacc cggatccgtt agcgtaccag agatgcgaga
     4621 ccaaaatgaa gcgagttgtc caatgcccca acggtgaata gcgcctgccg cggatacacc
     4681 cgcaactaaa cttacaactg tcaaaaaacc gcggcgggat agacgcttaa cttggaacat
     4741 gacgtcgcga gttcactcac ttcagtcagt aacagccttt tactgctgct gacgtttgcg
     4801 gacgacaatg
//
LOCUS       DASSWZ010000170         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_585898, whole genome shotgun sequence.
ACCESSION   DASSWZ010000170 DASSWZ010000000
VERSION     DASSWZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585898"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1245
                     /locus_tag="VFN58_04225"
     CDS             160..1245
                     /locus_tag="VFN58_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8917230.1"
                     /translation="MRLKQALVLLILAMLIEYSAPGFTVQAAAPAKPGFDEKAVADFY
                     RNKTVRIVVGFSAGGGYDQYSRLIARHLSKYIPGNPNIIVDNMAGAGSIIAANHTFNA
                     APKDGSMIGNISGPIILEQLFGNPAVQFDLAKFRYLAVPVSESYVMVVTRKPGVAKFD
                     ELIGPKAKQVVFGGIPGSTVEHAPILARDILGANIKLVLGYKGTADVRMAIEGGEVEG
                     FFNSWTSLKITSFDKVKNGEWLLLAQLAEKPIKDLIVPNVPTIPMITKTTEQNLLLKY
                     GTSTPNDFGKVYVLPPGVPADRAAALENAFARVFTDKELLADAEKGKLEIDPMIGEDI
                     HKLVVEFLGMSPELKAKLQGALKGGKK"
     gene            1287..2297
                     /locus_tag="VFN58_04230"
     CDS             1287..2297
                     /locus_tag="VFN58_04230"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917231.1"
                     /translation="MARFKRLSLLAAFFAFMLPVVATAQTETIDEVYKKALKEEGVLN
                     CYCSLAQINAEIIYPLFEKRFPGIKINHVDATSDKLAARAITEARGGRTIADVVEFGL
                     EDINKIHEQGLILEKAPAEAAQYPANLRGPYWVANNLIFFIGAWNTEKVKKEETPKFL
                     DDFGEPRWKGRLIAEPRDYEVLIGLTHKHKSLEKARAVLAKIADNNVEFHKGHSQLAE
                     LLVAGQAAACFTCYSHHYPARKKKGAPVDYMLSEGAAGIIAVAVLKNAPHPNTAWLFT
                     RWAASEEGQAIYAKGGRTPAHPKVEPTEPIRPKVIYAVGVEDLKQYTKYEKIWKEVFK
                     LR"
BASE COUNT          633 a          575 c          671 g          526 t
ORIGIN      
        1 gactacgcgg cgacccttaa gaggctggaa aacctataga catatttcag cgaactgcgc
       61 tgaagttaga gcatatcttc acgtctaaat aggtggaccg gagcggtgag gtagaaccgt
      121 cctaaaattc ggcggctaag ggtcgcccag gaggaaagaa tgcgactaaa gcaggcgtta
      181 gtcttactga ttttagcaat gttaattgag tattcggcgc ccggttttac cgtccaggcg
      241 gctgcgccgg caaagcccgg tttcgatgaa aaagcggtcg ccgacttcta ccgcaacaag
      301 acagtccgaa tcgttgtcgg cttttcggct ggaggcggtt atgaccagta ttcgaggctg
      361 atagctcggc atctatccaa atatattcct gggaatccaa atatcattgt ggacaacatg
      421 gcgggcgccg gcagcattat cgccgcgaat catactttca acgctgcgcc caaagacggg
      481 tccatgatcg gcaacatttc cggtccgatc attttggaac agctttttgg caatccggcg
      541 gtgcagttcg atctggccaa gtttcgctac ctggcggtgc cggtgagcga gtcttacgtg
      601 atggtggtta cgcggaaacc tggtgtcgca aaatttgatg agctcatcgg cccaaaggca
      661 aagcaagtcg tcttcggcgg cattcccggc tcgacggtag agcatgcgcc aatactggcg
      721 cgggacattc ttggggccaa tatcaagctc gtgttgggct ataaggggac ggccgacgta
      781 cgaatggcca tcgaaggtgg ggaagtcgaa gggtttttca actcctggac ctcgttgaaa
      841 atcacgtcct tcgataaggt caagaacggc gagtggctct tattggcgca acttgcagaa
      901 aaaccgatca aggacttgat cgttcccaat gttcccacga tcccgatgat caccaaaacc
      961 accgaacaga acctcttgct taaatatggc acaagtacgc caaatgattt cgggaaagta
     1021 tatgttctgc cccccggcgt accggccgac cgggcggcgg cattggagaa tgcctttgcg
     1081 agagtgttta cggacaagga actgttggct gacgccgaaa agggcaaact ggagatcgat
     1141 ccaatgatcg gtgaggatat tcataagctc gttgtcgagt ttttaggcat gagcccggag
     1201 ctgaaagcaa aattgcaggg cgcgttaaag ggcgggaaga aatagtttaa ccaatctcgt
     1261 aaaacaatta aggaggagac acaatgatgg cgcgatttaa gagactcagt ttgttggctg
     1321 ccttctttgc tttcatgctt ccggtggttg cgacagcgca gactgaaacc atcgatgaag
     1381 tatacaagaa agcactgaag gaagaagggg ttctcaactg ctactgctcc ctcgcacaga
     1441 ttaatgccga aataatctat ccgctgtttg agaagcggtt tccgggcatt aaaatcaatc
     1501 atgtcgatgc cacctccgat aagctcgcag cacgggcgat tacggaagcc cgcgggggca
     1561 ggaccatcgc ggacgtggtt gaattcggcc tggaagatat caataagatt cacgagcagg
     1621 gactgatttt ggagaaagct ccagcggaag ctgcgcagta tccggccaat ttaagaggcc
     1681 cctactgggt tgccaacaac ctcatcttct tcatcggcgc ctggaatacc gaaaaggtta
     1741 aaaaagagga gacgcccaaa tttttggacg atttcggcga accgcgctgg aaagggcgac
     1801 tcattgcaga gccaagggat tatgaagttc tcatcggcct cacgcacaag cacaagagct
     1861 tggaaaaggc ccgggcggtt ctcgccaaaa ttgccgacaa taacgttgaa ttccacaaag
     1921 gacattcaca gctggccgag ttattggtgg ctgggcaagc ggcggcatgt ttcacttgct
     1981 attcgcatca ttacccggca cgcaagaaaa agggggcacc tgtagactac atgctctctg
     2041 aaggcgccgc gggaatcatc gcggtagcag tgctgaaaaa tgcgccgcat ccgaacacgg
     2101 cctggctttt tactcgttgg gcggcgtcgg aagaagggca agctatctat gcgaaagggg
     2161 gtcggacgcc agcgcacccc aaagtagaac ccaccgagcc gattcgcccg aaggtcattt
     2221 atgcggtcgg ggtggaagac ctgaagcaat ataccaaata tgagaagatc tggaaggaag
     2281 ttttcaagct acgctgattg gagaccagca aacaaacccg tgcgtttgtt cgtcttatct
     2341 attaagaaaa ggagacttta tgtttagagc taaaccagtg gtatctgcca gtgtcacagc
     2401 attga
//
LOCUS       DASSWZ010000171         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_586016, whole genome shotgun sequence.
ACCESSION   DASSWZ010000171 DASSWZ010000000
VERSION     DASSWZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586016"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..>2533
                     /locus_tag="VFN58_04235"
     CDS             79..>2533
                     /locus_tag="VFN58_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8917232.1"
                     /translation="MSTIQGTSPSKSIASVGTLNQLTETNQSQDALRESNFRLQLAVE
                     SANIGIYELKVKNGELLWDDRVRAQWGLPPGVPVDYNTFLKGVHPDDREATQAAIDRA
                     LDPNGDGRCRVEFRVIGIQDGIERWIVGTGQVFFENGQAERLVGTTFDITARKHAEQR
                     LRESEERFRTLADSSPVPIWVTDATGGVEFLNAAYSDFFGVTLEDVRGPKWAMLLHPD
                     DADPYVGAYYEALRQRKPFRGEARVRRGDGEWRWVDTRGVPRFSEGGEFLGHVGSCPD
                     VTEMKMAQQALRESEEQFRASFEISTVGMAQADPITGRLLRVNQRYCEMTGYSEAELL
                     QLSIRDITHPENRAADFERFQRLGRGEVSEYWAEKRYLRKDGGVIWVAATANLVRNAS
                     GQPTRTVAVITDITGRKQAEAALRESEAQFRASFELAAVGQAQVNPATGRFERINRKF
                     CEIVGYSEEELLNMAPADITHPEDRQPYVDIYPRFLRGEIEEFVIEKRYVRKDRKIVW
                     VSVAAKLIRDSDGRPLRTIGVFQDITERKRSEEELKQADRRKDEFIATLAHELRNPLA
                     PIRNAVEMLGQCQSSDPDFEWGRNVIDQQVKHLACLVDDLIDASRITRNRLIIKKERV
                     SLNDVINGALDATRLLIEQRGHLLTVALPVEPVYLNADPVRLTQIFMNLINNAAKYSH
                     ERGQIRVDAERKNDSVTVRVADNGIGISTDELSHLFDMFYQVDRSYDQAHGGLGIGLT
                     LVKRLVEMHGGTVEAKSAGIGQGSEFVVSLPVFVSSSAVLLDKDAVAQKYNTRRRILV
                     VDDYPSAAESLARRLKRFGN"
BASE COUNT          602 a          632 c          759 g          540 t
ORIGIN      
        1 aaacggctcc cctctaacga gggtagaacg gtctccattt ttggagccgt atagttgttg
       61 gtgtcaggag gaactgccat gtctaccatc caagggacaa gtcccagcaa gtcaatagct
      121 tcggtcggaa cgttgaatca actcactgag actaaccagt ctcaggacgc cttacgtgaa
      181 agcaatttcc ggcttcaact ggcggtcgaa tcggcaaaca ttggcattta cgaattgaag
      241 gtcaagaacg gtgagctttt gtgggacgac agggtgcggg cgcagtgggg tctgccaccc
      301 ggcgtgccgg ttgattacaa cactttcctg aagggtgttc atcctgacga tcgggaggct
      361 acacaagccg ccattgaccg ggcgttagat cccaatggag acggccgctg ccgcgtcgag
      421 tttcgcgtta tcggcattca agacggcatc gagcggtgga ttgtcgggac gggccaggtg
      481 ttcttcgaaa atggtcaggc tgagcgactg gtcggcacga cgttcgacat cacggcccgt
      541 aaacacgcgg agcaacgtct acgcgagagc gaagagcgat ttcgcactct ggccgacagt
      601 tcacccgttc caatctgggt gactgacgcc accggtggtg tcgagtttct caacgccgcc
      661 tactctgact tcttcggcgt caccctcgaa gacgtgcggg gacccaaatg ggcgatgctg
      721 cttcatcctg atgacgcgga tccatatgtc ggtgcctatt atgaagcact gcgccagcgc
      781 aagcctttcc gtggcgaggc tcgagtgagg cggggagacg gtgagtggcg ttgggtggat
      841 acacgcgggg ttccccgctt ctctgaaggt ggagaatttc tcggacacgt agggagttgc
      901 cccgatgtaa cggaaatgaa aatggctcag caagcgctgc gcgaaagcga ggaacagttc
      961 cgcgcgtcct ttgaaatctc cacggttgga atggctcagg ccgatcccat taccggtcgt
     1021 ttgcttcggg taaatcagcg ctattgcgaa atgacgggct acagtgaggc agagttgctg
     1081 caactctcca tacgggacat tacccatcca gaaaatcgag ccgccgactt tgaacgtttt
     1141 cagcgtctgg ggcgcggcga ggtgtcggaa tactgggccg aaaagcggta cttgcgaaaa
     1201 gacggaggag ttatttgggt tgcggcaacg gccaatctcg tgcgcaacgc gagcggtcaa
     1261 ccgacgcgaa ccgtggcggt cattactgac attacgggtc gcaagcaagc cgaagcggcc
     1321 ttgcgtgaaa gcgaggctca attccgcgcc agttttgagt tggccgcagt agggcaggca
     1381 caagtgaatc ctgccaccgg cagatttgag aggataaacc gaaaattctg tgaaatcgta
     1441 ggttatagtg aagaggaact cttaaacatg gcgccggcgg acatcacgca tcctgaggac
     1501 cgacagcctt acgtggatat ctatccgcga ttcctgcgcg gtgaaattga agaattcgta
     1561 atcgagaaac gttatgtgcg aaaggatcga aaaatcgtat gggtcagtgt cgctgcaaaa
     1621 ctaatccgcg attcggatgg gaggccgcta cggaccatag gcgtgttcca ggatatcacg
     1681 gagcgtaagc gatcggagga ggagctcaaa caggccgacc ggcgcaagga tgaatttatc
     1741 gcgaccctgg cgcacgaact gagaaatccg cttgcgccca ttcgtaacgc cgtggagatg
     1801 cttggccaat gccagtcatc agatcccgac ttcgagtggg gacgcaacgt gatcgatcag
     1861 caggtgaaac atttggcgtg cctcgtcgat gatctaatcg acgccagtcg cataacccga
     1921 aacagactca taatcaaaaa agagcgcgtc tccctcaacg acgtgattaa cggcgctctg
     1981 gatgcgacgc gacttttgat cgagcagcgc ggacatctac ttactgttgc gttgcctgta
     2041 gaaccggtgt acctgaatgc agacccggta cggctcactc agattttcat gaatctgatc
     2101 aataacgcgg ctaaatatag tcatgagaga ggacaaatcc gggttgatgc tgagcggaaa
     2161 aatgacagcg tgacagtgcg cgttgctgat aacggcatcg gcatttctac cgacgagttg
     2221 tctcacttat tcgacatgtt ctaccaagtg gatcgttcct atgaccaggc gcatggcggt
     2281 cttggcattg gcctgactct ggtgaagcga ctggtagaaa tgcacggtgg aactgtggag
     2341 gccaaaagtg caggtattgg tcaaggaagc gaatttgtgg tttctctgcc ggtgtttgtc
     2401 agttcgtccg cggtattact ggacaaagat gctgtggcgc agaaatacaa cacgcgcagg
     2461 cgaatccttg tggtcgatga ttacccaagc gcggcagaaa gcctggcgcg gcgccttaag
     2521 cgctttggta acg
//
LOCUS       DASSWZ010000172         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_288847, whole genome shotgun sequence.
ACCESSION   DASSWZ010000172 DASSWZ010000000
VERSION     DASSWZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288847"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..1114)
                     /locus_tag="VFN58_04240"
     CDS             complement(359..1114)
                     /locus_tag="VFN58_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET8917233.1"
                     /translation="MMTVEEFAAKIRESDEIVVFTGAGISTESGIPDFRSPGGLWTRY
                     KPVTFQDYMSSEAARIEAWKRRLETYEAHKNARPNIGHYFIQSLDQKGKLIGLITQNV
                     DGLHGEAGLADDKIVELHGTNRRIICLKCDKSFDPDQVIKRLVGDFASPKCDACGGIL
                     KAATVSFGQAMPPKAMRQAQEWTEGAAIFMVMGSSLQVQPAASFPVLAKRNGALLAII
                     NREPMPLDEHADFVHHGAIGEFCGRFGEMIADG"
     gene            complement(1174..2403)
                     /locus_tag="VFN58_04245"
     CDS             complement(1174..2403)
                     /locus_tag="VFN58_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917234.1"
                     /translation="MTGKKSAAEKRERFNRDQPNLNTAPFWPQLGPVVFLAMIFLINF
                     IARIILSPLLPTIEKELGISHGQAGSFFFLISAGYVIGLLGSGFLASRSSHKITIVIS
                     TGGVGLALLGISVVSSLWAMRIGLFGLGFAGGLYIPSAIATITSLIERPHWGKAIAVH
                     ELAPNLAFFAGPFVAELFLSWSTWRLALNSLGMASLIASFAYFRFGRGGEFAGESPAS
                     SAFGLLVHSPSFWIMAVLFGLGVSSTIGVYAMLPLYLVSERRIDLSWANTVVALSRSY
                     GPILGVLGGWVSDRLGPKQTMVMSLTFTGIATLLVGLVSTVWISSVVLLQPLLAVWFF
                     PAAFAALAATTPAAARNLAVAFTIPFGYLIGGGVVPTFIGIMGDTGSFAVGFIVTGTL
                     ILLGGTLALLLKLPANK"
     gene            complement(2738..3109)
                     /locus_tag="VFN58_04250"
     CDS             complement(2738..3109)
                     /locus_tag="VFN58_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917235.1"
                     /translation="MPEQISKYPDVTLQVLRESGAVCGQGAPQNILKQCPRDRFCSLR
                     TGEICVYGINDIPKMTQITSQELAKVVCPQAQKAALFSAGISGLEAALIFTTFFSGLA
                     AGKYWQKPRWRVSRPPTDPPN"
     gene            complement(3191..>3626)
                     /locus_tag="VFN58_04255"
     CDS             complement(3191..>3626)
                     /locus_tag="VFN58_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET8917236.1"
                     /translation="RFNQPTTVDRAINKLFGLFVGVGLGLPHNYLLQVRGRTSGRIYS
                     TPVDVLDRDGKRYLVAPRGYTQWVRNAIASGTVSLKKGRRSEEFGIRLLSDDEKPEIL
                     KSYLDHYKLTVQRYFPVAAGSPTEAFRPLTGEYPVFELIPRR"
BASE COUNT          863 a         1043 c          925 g          795 t
ORIGIN      
        1 aagcttcgcg actcggtcaa caaagcatgg ttaaagatgt ttcctgacga acatgatcga
       61 cccgcgcgtc acgcgctgaa agcagacctg cgcggccagt tcttgttcca gatagaggta
      121 atcgcggttc tctaattttt caaacagtcg gctgacaaag gggattttta gagcccgtaa
      181 agctttttgg aattgtcgta actgatcttg cgtttcaacg cttcagaaat atccttccgc
      241 tccaaagaca tcgctttcga tttctcggat atgaccctca gcatcgacca tttttgattc
      301 ggtcattgtg ctctttctcc ttgtctccct gtctctcttt ctctttgtct gcctgttgtc
      361 acccgtcggc gatcatctcg ccaaatctgc cgcagaattc tcctatggca ccgtgatgga
      421 cgaagtccgc atgctcatcc agcggcatcg gctcgcggtt aatgatcgcc agcaacgcgc
      481 cgttgcgttt ggcgaggacc ggaaacgagg cggcgggctg cacctgtaac gatgatccca
      541 tgaccatgaa aatagccgcc ccttcagtcc attcttgggc ctgccgcatc gccttcggcg
      601 gcatcgcctg gccgaacgat acggtcgccg ctttaagaat accgccgcac gcgtcgcact
      661 tgggagaggc aaaatcaccc accagccttt taatgacttg gtccggatcg aaggatttgt
      721 cgcacttcag acaaatgatc cgtcgatttg tgccgtgcag ctcgacaatc ttgtcatcgg
      781 caagtcccgc ctcgccgtgc agaccgtcca cattctgcgt gatgaggccg ataagcttgc
      841 ctttctgatc aagggactgg ataaaataat ggccgatgtt gggcctggcg ttcttgtgcg
      901 cctcgtaagt ttccaacctt cttttccagg cctcgatgcg cgccgcttcg ctgctcatat
      961 agtcctggaa agtaaccggc ttatagcgcg tccagagccc gccgggacta cggaaatccg
     1021 gaattcccga ttccgtgctg attcccgcgc cggtgaaaac gacgatctcg tcactctctc
     1081 tgattttcgc cgcgaattct tctacggtca tcataagcta ttcttataga acagcccgcg
     1141 tgtcgatgac aaccggcgct tcagagtgct ttctcattta ttggccggta gtttcaataa
     1201 caatgccaat gtgccgccta acaaaatcaa agttccggtc acaataaaac cgacagcaaa
     1261 ggaaccggtg tcgcccatga tcccgatgaa agtcgggaca acccctccgc cgatcaaata
     1321 accgaaagga attgtaaagg ccaccgcgag attacgcgcg gcagctggag tagtcgcggc
     1381 gagggcagca aacgccgcgg gaaagaacca gaccgcaagc agtggctgca acaagacgac
     1441 gctgctgatc caaacggttg aaaccagtcc caccagcaag gtggcgattc ctgtgaaagt
     1501 taaactcatc accatggtct gttttggacc caaacgatcc gacacccaac ccccgagaac
     1561 ccccaggatc ggtccatagg acctggagag ggcaaccacc gtgttggccc agctcagatc
     1621 gatccgccgc tcgctcacaa gatagagcgg aagcatggca taaacaccga tcgtggagct
     1681 cacgcccaag ccaaacagca cggccatgat ccagaatgaa ggcgagtgaa ccagtaatcc
     1741 gaaagcgctc gatgccggcg attcaccggc aaattcgcct ccccggccaa agcggaaata
     1801 tgcgaaactc gcgatgagcg aggccatgcc aagagaattc aacgccaagc gccacgtcga
     1861 ccaactcaag aacaattctg cgacaaaggg cccggcaaaa aaagccagat tgggtgccag
     1921 ctcatggacg gcaatggctt ttccccaatg cggtcgttcg atcaacgacg tgatcgttgc
     1981 aatcgccgag ggaatataaa ggccgccggc gaaccccaag ccgaagagcc cgatgcgcat
     2041 cgcccataag ctactcacga cggaaattcc taggagcgcg agtccaacgc cgccggtgga
     2101 aatcacgatg gttattttgt gactcgaacg ggacgccaga aatcccgatc ctagcagtcc
     2161 gatcacataa cccgccgaga tcaagaaaaa gaaagatccc gcctggccgt ggctgattcc
     2221 caattccttt tcgatggtcg ggagtagtgg tgaaagaatg attctcgcga tgaaattgat
     2281 gagaaagatc atcgccaaga aaacaacagg ccccaattgt ggccaaaaag gcgccgtgtt
     2341 caggtttggc tgatccctgt taaaacgctc tcgcttttct gctgcacttt tcttaccagt
     2401 cacgtttgat gtatcctcgg cgcacaaggt taagtcttaa acctctacaa tgctctaatc
     2461 cgtaagccaa ctacaaaacg aagggagctt cttatgactg cggtcaaaaa aaataccgca
     2521 tggaagctta gccgcgcata gatacgcgac ctctaacgcg gcgtcgtttt cttgcgtcca
     2581 tgcctcccgc gagcgcgtcc acaatggctt ggcctttagt gcttgactac tttcaaggtg
     2641 aacgtaggtt tttcgcccaa gccgccgagc tttcgggtga cccgagatgg gaaactcgaa
     2701 tctcgaaact gtgcaacagc gaaaagcgct tggctgacta gttcggtggg tcggtcggcg
     2761 gtcgagaaac gcgccagcgc ggcttctgcc agtacttgcc cgcggcgagt cccgaaaaaa
     2821 acgtcgtgaa aatcagagcc gcttccaatc ctgagatgcc cgcggaaaat agcgcagcct
     2881 tttgagcttg cggacacacg actttagcca gctcctggga cgtaatctga gtcattttcg
     2941 gaatgtcatt tatgccatac acacagattt ccccggttcg aagtgaacag aaacggtccc
     3001 gcgggcattg ttttaggatg ttttgcggcg ccccttgacc gcaaacggca ccgctctctc
     3061 tcaagacctg cagcgttacg tctggatatt tcgatatttg ctccggcatg atggttgtac
     3121 ctccccgctc gatactgctg tatcgtaccc ttgaacggcc ttgccctgca caaggccaaa
     3181 accgccaagt tcatcgccgg ggtatcagct cgaagaccgg gtattctccg gtcaatggtc
     3241 gaaaagcttc ggtcggagag ccggcggcca ccgggaagta ccgctgcacg gtcagcttgt
     3301 aatggtcaag ataggacttc agtatctccg gcttttcgtc gtctgaaagt aaacggatac
     3361 cgaattcctc ggatcgccgg cctttcttga gcgataccgt accgcttgcg atggcgttgc
     3421 gtacccactg cgtgtacccg cgcggcgcca ccaagtatcg cttgccgtct cgatcgagta
     3481 catccacggg agtcgaatag attcgcccgc tcgttcggcc acgtacttgg agcagatagt
     3541 tgtgcggcag acctagcccg accccaacga aaagcccgaa tagcttgttg attgcccgat
     3601 cgacagtcgt gggttgattg aagcgc
//
LOCUS       DASSWZ010000173         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_741888, whole genome shotgun sequence.
ACCESSION   DASSWZ010000173 DASSWZ010000000
VERSION     DASSWZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741888"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..805
                     /locus_tag="VFN58_04260"
     CDS             488..805
                     /locus_tag="VFN58_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917237.1"
                     /translation="MYARNVRIKLKANDAPEFARILEKEIIPLLRKQEGFQDEMLFVA
                     PQRNEAIAISFWDKQVHAEAYNHIAYLDVLRILSKVVEEMPIVETFEVANSTSHEIAA
                     SAA"
     gene            874..1152
                     /locus_tag="VFN58_04265"
     CDS             874..1152
                     /locus_tag="VFN58_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET8917238.1"
                     /translation="MNVIVLIIVGALAGWLTGKAVEVEGRTIVVKESHATDAVYGVIG
                     ALIGQYLFFWIVIGQGNAFSSYATGVLGSITLVGAARLLAARWHRVRS"
     gene            1192..2652
                     /gene="sufB"
                     /locus_tag="VFN58_04270"
     CDS             1192..2652
                     /gene="sufB"
                     /locus_tag="VFN58_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET8917239.1"
                     /translation="MNTATYTTEELVNREYRYGFVTDIETDTVPRGLNEDIIRLISAK
                     KNEPSFMLDWRMKAYQHWVKLAKSQAEPKWANIHYPPIDYQDLIYYSAPKTSAEGLKS
                     LDEVDPKLLETYAKLGIPLSEQKWLSGVAVDAVFDSVSVATTFKEKLAELGIIFCPFS
                     EAVQKHAVLVKKYLGSVVPYSDNFFAALNAAVFSDGSFCYIPKGVRCPMELSTYFRIN
                     AAETGQFERTLIIADEDSYVSYLEGCTAPMRDENQLHAAVVELIALDNAQIKYSTVQN
                     WYPGDKDGKGGIYNFVTKRGKCLGINSKISWTQVETGSAITWKYPSCILQGDNSVGEF
                     YSVALTNNYQQADTGTKMIHIGKNTKSTIISKGISAGHGQNTYRGLVKVMKGATGARN
                     YSQCDSLLLGDKCGAHTFPYLEVLNSSSQVEHEASTSKIGEDQLFYCRQRGISPEDAV
                     TLIVNGFCKQVFRKLPMEFAVEAQKLLGVSLEGSVG"
BASE COUNT          656 a          684 c          742 g          588 t
ORIGIN      
        1 ctccaagtcc ataagacacc tcccttaaat gcaagttgag aacacgcagt ttggaacacg
       61 caggtttaat ttcccgcagc agtgacaagt ccgactattg ttttgcgaca gagcattctt
      121 ttgatcttga tgctgcgctg atccagcgac agttgctgtc taatattgaa caatcttgaa
      181 aataattatt accaggcccg caggcaggca cacccagaga gtccccctcc gactcatcgc
      241 tatatagccg aaatttcgac ttcaggcgcc agcgcccttt cccgagacca ccagccaacc
      301 ggggtgatcc catggatcag gcgataattc ttttgaagac cgtctaatat tggacagcaa
      361 tccctcgccg gtttgatgta agttcataag gattcaaggc atagggttcg ctccgcggtt
      421 tacctgaaat tcttcgagta agcctacaat cctcacccct ataacagtcc acgccgggag
      481 gtttggaatg tacgctcgca acgtcaggat taagctcaag gccaatgacg cgccggagtt
      541 tgcgcgcatt ctggagaagg aaattattcc cctacttcgc aaacaggagg gatttcagga
      601 tgaaatgctc ttcgtcgcgc cgcagcggaa tgaggcgatc gcaattagct tctgggataa
      661 gcaggttcat gcggaagcct acaaccacat agcttatctt gatgtcttga ggatcttatc
      721 gaaagtggtt gaagagatgc ccatagtgga gacctttgag gtcgcaaatt cgacttccca
      781 cgagatcgct gctagcgcgg cctagacaga ccttgcggtc cggagagggg aaacacttct
      841 ccggatggtt attacccaac ggaaggaggc ttcatgaatg tcatagttct gatcatagtc
      901 ggagcattgg ccggttggtt gaccggaaaa gcggtcgagg tcgagggtcg cacaatcgtc
      961 gtgaaagaga gccacgctac agacgcggtg tacggggtca tcggcgccct gatcgggcaa
     1021 tatctatttt tctggatcgt tatcggtcaa ggcaatgcct ttagcagtta tgcaactggc
     1081 gttcttggct cgatcactct cgtgggagcc gcccggctgc tagctgcacg gtggcaccgt
     1141 gtcagatctt aaagaggcgg aacccgtctc aagaaaagga aaactgaagc catgaacact
     1201 gccacataca cgacagagga gctggtcaac cgggaatata ggtacgggtt tgttaccgac
     1261 atcgagacgg acaccgtgcc tcgcgggctg aacgaagata tcatccgctt gatttcagcc
     1321 aagaaaaacg agccgagttt catgctggac tggcgtatga aggcctacca gcactgggtg
     1381 aagctggcaa aatcacaagc agaaccaaaa tgggccaaca tccactatcc gccgatcgac
     1441 taccaggacc tcatctatta ctcggcgcca aagacgagcg ctgaggggct gaagagcctc
     1501 gacgaggtag atcctaaact gctcgagacc tatgcgaagc tcggtatccc cctgtcggag
     1561 caaaagtggc tcagcggcgt cgcggtcgat gcggtctttg acagcgtctc cgtggcgacg
     1621 acgttcaagg aaaagctggc ggagttgggg attatctttt gtcccttttc cgaggcggta
     1681 cagaagcatg cagttttagt gaaaaagtat cttgggtcgg tggtacccta cagtgacaac
     1741 ttcttcgccg ccttgaacgc ggcggtcttc agtgacggat ccttctgtta catccccaaa
     1801 ggcgtgcgct gtccgatgga gctatcgact tactttcgca tcaatgccgc tgaaacgggc
     1861 cagtttgagc ggacgttgat cattgccgat gaggacagct acgtgagcta tctggaagga
     1921 tgcaccgcgc cgatgcggga tgagaatcag ttgcatgccg cggtcgtgga gctgatcgct
     1981 ctcgacaacg cgcagatcaa atactcgaca gtgcagaact ggtacccagg cgacaaggac
     2041 ggcaagggtg ggatctacaa ttttgtcacc aaacgcggca agtgtctggg tataaactcc
     2101 aagatctctt ggacccaggt ggagaccggc tcggcgatca cctggaaata tccgagctgc
     2161 atcctgcagg gtgacaactc ggttggtgaa ttttactcgg tggcactcac caataactat
     2221 cagcaggcgg ataccggcac caagatgatc cacatcggga aaaacaccaa gagcaccatc
     2281 atctcgaagg ggatctcggc gggccacggg caaaacacct atcgggggct cgtgaaagtc
     2341 atgaagggtg ctaccggcgc tcgtaattat tctcagtgtg actcgttgct gctcggggat
     2401 aagtgtggcg ctcatacctt cccctacctc gaggtgctga acagctcgtc gcaggtggag
     2461 cacgaggctt cgacctcgaa gatcggcgag gatcagcttt tttactgcag gcagcgcggg
     2521 atttcaccgg aggatgcagt cactctgatc gtcaatggct tctgcaaaca ggtgtttcgg
     2581 aagttgccga tggagtttgc cgtggaagcg cagaagcttc tcggcgtgag tctcgaaggc
     2641 agcgtcggct aaaacaagga gtattcacca
//
LOCUS       DASSWZ010000174         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_826607, whole genome shotgun sequence.
ACCESSION   DASSWZ010000174 DASSWZ010000000
VERSION     DASSWZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826607"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..641
                     /locus_tag="VFN58_04275"
     CDS             249..641
                     /locus_tag="VFN58_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917240.1"
                     /translation="MERATNRPWFAIVWFAIWGSFQAYAVTSVFAGTWRKPEAFPEEA
                     YFNLIYPDMFFIPLYLLTAGLLLLRHWLGSILAFIAGGGIIYVMIYLLALSGLSGAAN
                     LIADGIFLLCTLISLWQVGHHSNFRKPV"
     gene            753..1616
                     /locus_tag="VFN58_04280"
     CDS             753..1616
                     /locus_tag="VFN58_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8917241.1"
                     /translation="MVDSIRRKILKTGAAATVMAAARRVFGQQIGQGGATMSLYEKGP
                     VRISYEEAGSGFPLLLVPGGGLNSTIAGLKRGGPFNAIEEFKGEYRCIFSDMRNANGG
                     QSSGPLEIDRPWDAYTDDHIGVMDHLRIDKFMVMGFCIGGPLVWNLLKRAPDRIVAAV
                     LAQPSGFRPELPNHSYENNIKGWGPELVKRRPDITMEMVDKFLNKMYRTNADFVWTVT
                     RDFVRNCRTPVLILPDDTPPHPYAVAMEAAMLAPQAEVSMFPWKEPKERIPLAVRQIR
                     SFLRAHRLASV"
     gene            1886..2050
                     /locus_tag="VFN58_04285"
     CDS             1886..2050
                     /locus_tag="VFN58_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917242.1"
                     /translation="MKETVAMRLSGHRTRSVFDRYNIVSEDDLIDASKKLEAFLAEAV
                     KESKVEKLKK"
     gene            2697..2927
                     /locus_tag="VFN58_04290"
     CDS             2697..2927
                     /locus_tag="VFN58_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HET8917243.1"
                     /translation="MSAKRRSKNTPPDGLPIYRILTGPDDAAFCHRVSEALALGYTLY
                     GSPAVTFNGKDIIVAQAIIWPSIQGATAKLKR"
     gene            3011..3268
                     /locus_tag="VFN58_04295"
     CDS             3011..3268
                     /locus_tag="VFN58_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917244.1"
                     /translation="MKIPSVLAGVAGEYFVAAELSRRGHIASISLRNTRGIDIIATNK
                     AASRSITIQCKTNQLTRKMGAAHDGNDWTRLSALHVFRFGY"
BASE COUNT          844 a          963 c          986 g          846 t
ORIGIN      
        1 tccatttttc tggcttagat caagccaaat tcggcgtcag tttcgtacat acatcatccc
       61 tacccacttt gcagccattc actattgact atccccaagg tgacagaggg aaatgaaatc
      121 tgtgtgcggg tgactaaagg cttcccagat cttcccgaga cttgattgct ctcaggcttt
      181 agggataaaa cgctatacgt tagaccgaca tccttcgagt cgaggatcta ctgggaaatt
      241 ctccaacgat ggaacgagca acgaataggc cgtggttcgc gattgtctgg tttgcaatct
      301 ggggctcgtt tcaggcgtac gcggttactt cggttttcgc tggaacgtgg cgaaagccag
      361 aagcatttcc cgaggaagca tacttcaatc tgatctaccc tgacatgttt ttcattccac
      421 tttacctgct taccgcagga ctattgctgc tgcggcattg gttgggcagt atcttggctt
      481 tcattgctgg cggtggaatt atctacgtga tgatttacct gttggcgctt tctggactat
      541 cgggggcagc gaatctgatc gctgacggga ttttcttgct gtgcaccctc atatcactat
      601 ggcaggtggg tcaccattcc aatttccgaa aaccggtcta acaaaacgct ggagccgccc
      661 ttcgcggcaa agccgctcgg gcgccctccg cgcaaacaac gcgcggctca gccctttgag
      721 ctgttagccg gccgaaagtc aggagggcgc cgatggtgga ttcgatcaga cgcaaaatac
      781 tgaaaacggg cgctgcggca acggtgatgg ccgcagctcg gcgagtgttt ggtcaacaga
      841 ttgggcaagg aggagctacc atgtcgttgt acgaaaaagg ccccgttcgc atctcctatg
      901 aggaggccgg ttccggcttc ccgttgttgc tcgttcccgg cgggggattg aattcgacga
      961 tcgccggctt gaaacgcggc gggcctttca atgcgatcga ggagttcaag ggagaatacc
     1021 gctgcatttt ctcggacatg cgcaatgcca atggcggcca gtcctccggc ccgctcgaga
     1081 tcgaccggcc gtgggatgcc tataccgatg accatatcgg tgtgatggat cacttaagaa
     1141 tcgacaagtt catggtgatg ggcttctgca ttggtggtcc tttggtttgg aacctgctga
     1201 agcgggcacc tgatcgtatc gtcgcagccg tgctggcgca gcccagcgga ttccggccgg
     1261 agctgcccaa ccactcctac gagaacaaca tcaagggctg gggtccggaa ttggtcaagc
     1321 gccggcccga catcacgatg gagatggtcg acaaattcct gaacaagatg taccgaacca
     1381 acgccgactt cgtctggact gtgacacgcg atttcgtgcg caattgccgg acgcccgtat
     1441 tgatcttgcc ggatgatacc ccgccgcatc cgtacgccgt cgccatggag gccgcgatgc
     1501 tcgcgccgca ggcagaagtg agcatgtttc catggaagga gccgaaggag cggattcccc
     1561 tggcggtgcg ccagatacgt tccttcctcc gggcgcatcg gctcgcttct gtttagacgg
     1621 cggtgtcaat accacaaggg tggacgcccg gtgaccgcgt gaaacattgc tcagccgtct
     1681 aacccttatg aggccctcgg ttgtcaaggg cacaggcctc cgttgacgaa aagacgttcg
     1741 ccttagtaac ggcctcgctc tcaagtaact gcgctctgac tttatttcca aattcaggtg
     1801 ctaggcgatc gccgcttgcg cggcatgcgg cctcctacct cacgatcttc gtcgatctgc
     1861 tgttcggaat ttcattcgag ctggcgtgaa agaaacagtt gcgatgagat tatccggtca
     1921 tcgaactcgt tcggtgtttg accggtacaa catcgtcagt gaagacgacc tgattgacgc
     1981 atcgaagaag ctcgaggctt ttctcgcgga agcagtgaag gaaagcaagg tcgagaagtt
     2041 aaaaaagtag ttgcaatctc cggagcggac cagaacggaa cattgacacg aaagcccaac
     2101 tattgcgagg ccgcttgctt tttcttcgac aatggaaggt gtatgttctg cccaggttca
     2161 ggcacaaaac gagcacaacc gcgatcggcc ctcgccttgc cagtggcctt tgccgatatt
     2221 aacatcgcga aagacatacc cgtataatag ttagatgggg atcaagacga agccatgacc
     2281 ctatcgcaga tcctgtccct catgtggagt gatttaatat ttgggatact ggggccggct
     2341 ttgtacattg atctatttgt cgttcttccg ttcataattc ctcgaaaacc ggcggcacga
     2401 tgggtggcaa ttgtggcatt attggttatc gtctttaagt ttctcggcta catgctaaga
     2461 gttctacccc cccagaccta ccgaagaaca ataggtggtt cagtttggca ttcgtctagc
     2521 agatgcaaat attggcctac ggaaaatttc gatgaagttt ctgaaagccc acaatttggc
     2581 gtctgctcca tctgtgtaaa gattgagaat agagctggcc gatacacagg cggctttaac
     2641 cgatactaat atcgcgaacg acatacccgt atactcatca atagttaggg tgcactatga
     2701 gcgctaaacg aagatcgaag aacacaccgc ctgacggtct acccatctat cgcattctca
     2761 cggggccaga cgacgcggcc ttctgccaca gggtcagtga agcgcttgcg ctcggctaca
     2821 cactttatgg ttcaccggcc gtcacgttta acggcaaaga tatcatcgtt gcgcaggcaa
     2881 ttatttggcc ttcgattcaa ggcgcaaccg cgaagttaaa gagatgaacc cttatatcgc
     2941 ctgcaggcct aaccccacag ttgagagaga cgctggacac cgccagccgt aggctgctat
     3001 cgcaggcgcc atgaaaatcc catcagtcct cgctggcgta gcaggtgagt atttcgtcgc
     3061 tgcggagttg tcacggcgtg gacatatcgc gtccatctca ctccgcaaca cgcgcggcat
     3121 cgacataatt gccaccaaca aagcggcgtc acgttcgatc acgattcagt gcaagaccaa
     3181 ccagttaacc cgcaagatgg gcgccgccca cgacggtaac gattggacaa ggctgtccgc
     3241 gctgcatgta tttaggtttg gatattgaac gagaagagtg aaaactttgt ttcagacgcc
     3301 ctcagaagta gttacaatct ccggagtgga ccagaacgga acattgacag gaaagtccaa
     3361 ccatttcgag gctcgcttgc tttttagccg acaatggaag atgtatgttg cccaggttca
     3421 ggcacaaaac aagcacagcc cgccgtgagg cgaaaggcac gaggcgtgag gagaaatgaa
     3481 taagcggaaa ctccgtagct ttgctctatg cgctttgctc cttgcatttt gggaatctgc
     3541 cgaggcgcag cagccgaaga aagtctatcg gatagggtat ctatcgtcgc tcgacccggt
     3601 tagtgagtcc gctcgtgccg caggaattcg actggcact
//
LOCUS       DASSWZ010000175         7201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_911512, whole genome shotgun sequence.
ACCESSION   DASSWZ010000175 DASSWZ010000000
VERSION     DASSWZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911512"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFN58_04300"
     CDS             complement(<1..201)
                     /locus_tag="VFN58_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917245.1"
                     /translation="MAQKRNSFVLLYGTAVVPLLAGTLLHSALGMPRAAAASLAALDN
                     RPPSFSAIAKKTMPVVVNISTTA"
     gene            347..1699
                     /gene="mgtE"
                     /locus_tag="VFN58_04305"
     CDS             347..1699
                     /gene="mgtE"
                     /locus_tag="VFN58_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET8917246.1"
                     /translation="MKPAPIDHDTVREMLRTATPDRVLRFIGQRHPADIALLFKGLEP
                     PEVKQLFDILFSARRADKALKELPPELLPDILALIDDDKLSRMIARADPDDAVTFIDS
                     LPEERRERIIALLDPERRAEVRELISYPEGSVGRIMTTDFMTFLPSTTAQGAIDKIRE
                     RGELETFFYLYVIDEAGKLVGVVPLRNLVVAPPNRLLGDMMIADPIKANVMMDQEEAA
                     RLVSKYELLALPIVDESGRLEGIITVDDVIDIIEKEATEDMYKMAGLAEEDRVFTPVS
                     RSVRMRLPWTILNLMTASLAASVVGLFEGTLHEIIALVTFMPVIAGVGGNGATQTATV
                     IIRAIALGELEFSSAWKAVVKQVLVNICIALAAGGVIAVAAFLWKGNPFLGLVLASAM
                     ILNLGLVAGFAGAVIPLVLKALKFDPAMGAGIIVTGLTDAFGFLSFLGLATLFRGYLS
                     "
     gene            complement(1696..2673)
                     /locus_tag="VFN58_04310"
     CDS             complement(1696..2673)
                     /locus_tag="VFN58_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET8917247.1"
                     /translation="MKKAKPLLVLLMKLLVSAGLLAFFFTRIHFERFLYTLAGADFGY
                     VAVGLVVYLAAQFVAALRWAILARPLGFDTRYRDFSLYYLIGMFFNLFAPSTVGGDVT
                     RVYYLARDGEKSPEKGWAVSTIPAAVSVFMDRAVGMAVLVWLGAAGLALFPYYAVPSA
                     IRSLTFALSLALIAVGLLVPLLRRILPDDSHPIVVKLRLAMRAYRAHWGVIPQAVLIS
                     LVLHLVQAWMHLLMGKALQINVPFSFCLILYPLVGTFAALPVSLNGIGLREGGYLFLL
                     GLIGINAEKGIAFGLLLFLIVVVDSLIGGLLFLVKKSPKPADDAFATSI"
     gene            complement(2670..3332)
                     /gene="purN"
                     /locus_tag="VFN58_04315"
     CDS             complement(2670..3332)
                     /gene="purN"
                     /locus_tag="VFN58_04315"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET8917248.1"
                     /translation="MRRQVPIGVLISGSGTNLQSIIDAIEGKELEAKIEVVLSNRADA
                     YGLVRAKKHGIPVEVLDHKRFASREAFDQAVVDILRAREVELVVLAGFMRLLSPVFVS
                     AYSNRIMNIHPALLPAFPGLHVQKKAVEHGVRFSGCTVHFVNEECDEGPIIIQAVVPV
                     FPEDTEESLAGRIQEQEHRIYPRAIQLYSEGRLRIEGRKVFVDGLARKEDQVLVLPPL
                     QK"
     gene            complement(3343..4368)
                     /gene="purM"
                     /locus_tag="VFN58_04320"
     CDS             complement(3343..4368)
                     /gene="purM"
                     /locus_tag="VFN58_04320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET8917249.1"
                     /translation="MTYAKAGVSIEAGDELVRRIGPIASRTKKPGVLAGVGGFSALFD
                     LKSRGYRHPVLVSSTDGVGTKLKVAFMMKKHDTVGIDLVAMGVNDIITQGAEPLFFLD
                     YFVCGKLDVDVAEVVVGGIAAGCRQAGCVLIGGETAEHSGDFPNGEYDLAGFVVGVIE
                     RKRVLNSKSIVPGDVLIGLPSSGLHSNGYSLARKVLLDVGRLKLRQQVPELGRSLGEE
                     LLEPTRIYARMTRGLFAKYPIKGAAHITGGGIPGNLPRILPEGRRAWIDRRTWPVPPV
                     FDLIQKIGRVSRDEMDRTFNNGLGMILVIGKRQVDDVMSMLKKMRERHFMIGEIKKGR
                     REVTFVS"
     gene            complement(4466..5257)
                     /locus_tag="VFN58_04325"
     CDS             complement(4466..5257)
                     /locus_tag="VFN58_04325"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8917250.1"
                     /translation="MLLIAVQNVSRVFTSGAKTVHALENVSFQIQAGDFVSLVGPSGC
                     GKSTLLKIVSGLLPASSGSVAVSDKPVDEPLENVGMVFQSPVLLKWRPVLGNVLLPVE
                     FAKLDLASHTERARSLLKLVGLEGFEEMYPHQLSGGMQQRVSLCRALVMDPQLLLMDE
                     PFGALDAMTRDELDIELLRIWQDRRKTVLFVTHSIQEAVFLSDHVIVMSARPGRLLEQ
                     IAIQLPRPRTMEMMSTTQFGEYTLKIRALLQGVDGIPAAIPGSTA"
     gene            complement(5284..6306)
                     /locus_tag="VFN58_04330"
     CDS             complement(5284..6306)
                     /locus_tag="VFN58_04330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917251.1"
                     /translation="MALLKTHFPLWSPAIIAVVVVVLISFAHVGSDAAETKTGTIRLD
                     FIIGGKHAPWFVALEKGFYAKRGLTATIQAGSGSADTVRAIAAGGSDFGFADMSTAIV
                     ARTRGTPVLTVAQFGYVPATIMWREDTAIKKLKDLEGKSWAISPGQAQWYLMPAYCRI
                     NKIDFKSIKIQETAAPIQPAALVAKRTDFIVMYRASNDEVAELAATKQGMKLNRIFMK
                     DTGLDIYGTGLIVKEDDIKQRPDLVRAYVEGTMEGLRYTRDHQEEALQILLKQKPELD
                     LALTTLQLKNALYEVFIPPESVQSGLGFMKPDIMDKTVRITNEYFDVGRKVTAAEVFT
                     NRFIKQ"
     gene            complement(6328..7104)
                     /locus_tag="VFN58_04335"
     CDS             complement(6328..7104)
                     /locus_tag="VFN58_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8917252.1"
                     /translation="MKPSVKNVIEPLIFFAVLLILWEMLVEVIKVPSFILPPPRDLWM
                     SFIKKLPILGNHSLITFTEAVGGFTFSLVLGVTFAIAVVYSRHLQNTIYPLIVILYAM
                     PKSAFAPLMVIWVGYGLMSKIAIAFLVAFFPVVVNTVVGLKEVEPELLELARINRGSQ
                     FDVFKKIRLPNAIPFMFAGIKVAIVLSVTGAIVAEFVAANEGLGYLVLQANYSLDTAF
                     ALVVLLILAVLSLGLFWLVEIVQRKIAPWSAEVRQVEGSF"
BASE COUNT         1759 a         2056 c         1887 g         1499 t
ORIGIN      
        1 cgctgtcgtc gagatgttca cgacaaccgg catggttttt ttagcaatgg cggaaaatga
       61 cggcggacga ttgtccaggg ctgcgaggct ggccgccgcc gcccgtggca ttccgagagc
      121 gctgtgcaaa agggtccccg ccagcaacgg tacgaccgcc gtaccgtaca agaggacgaa
      181 tgaatttcgc ttttgcgcca tctgttttcg tcgcctgcgc gggcttccgc gctagggcaa
      241 gatagcaaat tttcctcgta cttataagcg ggcaaatggc atcataaatt tgcaaattct
      301 cataccaatg gttatgaaag aacgagcctc attcccgagt aacagcatga agcctgcccc
      361 cattgatcat gacaccgtac gggagatgct ccgcaccgcc acgccagacc gtgtcctgag
      421 atttattggc cagcgccatc ccgctgatat cgcactgttg ttcaaaggtc tagagccacc
      481 ggaggtcaag cagcttttcg atatcctctt ctcggcgcgg cgcgccgaca aagcgcttaa
      541 agagctccct cctgaactgt tgcccgacat cctggcgctg atcgacgacg acaagctaag
      601 ccggatgatt gcccgggccg accccgacga cgccgtgact ttcatcgaca gccttcccga
      661 agagcggcga gaacgaatta tcgcactctt ggacccagag agacgcgctg aagtccgcga
      721 gttgattagt tatccggaag gaagcgtcgg gcgcattatg acgacggatt tcatgacctt
      781 cctgccgagc accaccgccc agggcgcgat cgataagatc cgcgagcgcg gcgaactgga
      841 aacgttcttc tatctctacg tgattgacga ggccggcaaa ctggtcggcg ttgtcccctt
      901 gcggaatttg gtggtggcgc cgccgaatcg tctcctgggc gacatgatga tcgccgatcc
      961 gatcaaagcc aacgtcatga tggatcaaga agaagcggcg cgcttggtct caaaatacga
     1021 gttgcttgcg ttgcccattg tcgatgaatc aggacgactg gagggaatta ttacagttga
     1081 tgacgtgatc gacattatcg agaaagaggc caccgaagac atgtacaaga tggcggggtt
     1141 ggcggaggag gatcgtgtct tcacgccggt ctctcgctca gtaagaatgc ggctgccatg
     1201 gaccatttta aacttgatga cggcaagctt ggcggcctcc gtggtaggat tattcgaagg
     1261 gaccctgcac gagatcatcg ctctggtcac tttcatgccc gtcatcgcag gcgtcggcgg
     1321 gaacggcgcc actcagacgg cgacggtcat tattcgcgcg attgcactgg gtgaactgga
     1381 attttcttct gcttggaagg ccgtcgttaa gcaagtcctg gtcaatatct gtatcgccct
     1441 cgccgcgggt ggcgtgatag ccgtggctgc ctttctctgg aaaggcaatc cctttctcgg
     1501 attggttctt gcgagcgcaa tgattctcaa cctcggcttg gtggcaggct ttgcgggagc
     1561 agtcattccg ctggtattga aagccctaaa attcgatccc gccatgggag caggcattat
     1621 tgtaaccggc ctcacggatg ccttcggttt cctttcgttt ttaggtctcg ccactctatt
     1681 ccgcggctac ctgagctaaa ttgacgtcgc gaaagcatca tcggccggct taggactctt
     1741 cttcactaaa aacagcagtc caccgatcaa gctatccacc accacaatca ggaacagcag
     1801 caacccgaag gcgatgccct tctcggcatt gatcccgata agaccgagca agaaaagata
     1861 tcccccttcg cgcagaccaa tgccgttgag actgacggga agtgcggcga aggtcccgac
     1921 aagcgggtac aggatgagac aaaaggaaaa cggaacattg atttggagag ctttgcccat
     1981 caagaggtgc atccaagcct gaaccaggtg aaggacaagc gaaatgagta ccgcctgagg
     2041 gataactccc cagtgggcac gataggctct catggcgagt ctcaatttga caacgattgg
     2101 atggctgtcg tctgggagaa tacggcgaag caaaggaacc agcaaaccca cggcaatcag
     2161 cgccagggag agcgcgaagg ttagcgaacg tattgccgat ggcacagcgt agtaagggaa
     2221 taacgcaagc ccagcggctc ccagccacac caaaaccgcc atcccgacgg cacgatccat
     2281 gaataccgac acggctgccg gaatcgtgga gacagcccaa cccttctccg gactcttttc
     2341 tccatcccgg gcaagatagt aaacccgtgt gacgtcgcct cctacggtac tgggcgcgaa
     2401 gagattgaaa aacatcccga tgagatagta cagactaaaa tccctatagc gagtatcgaa
     2461 acctaacggg cgagcaagaa tcgcccatct cagggcagcg acgaactgtg cggcgagata
     2521 gacgactaag cccacagcga cgtagccaaa atctgccccg gcaagggtat ataagaaccg
     2581 ctcgaaatgt atgcgtgtaa agaaaaatgc caaaagcccg gcgctgacca ggagcttcat
     2641 caaaagaact aacaacggtt tagctttttt catttttgca ggggtggcag aacaagaacc
     2701 tgatcctctt tccgggcgag gccatcgaca aagaccttgc gcccttcgat gcgcaatcga
     2761 ccctcgctat aaagttggat cgcgcgggga tagatgcggt gctcttgctc ctggatacgg
     2821 ccggcgagag actcctccgt atcctcggga aacaccggaa caacggcttg aatgatgatg
     2881 ggaccctcgt cgcactcttc gttgacaaag tgaaccgtgc agccagagaa gcgcacgcca
     2941 tgctccacgg cttttttctg cacatggagt ccgggaaatg ccggaagcaa tgccgggtga
     3001 atgttcataa tgcggttgga ataagcgctc acgaatacgg gggacaggag ccgcataaac
     3061 ccggcaagca caaccagttc cacttcacgg gctcgcaaaa tatcgaccac cgcctgatca
     3121 aaggcctctc gactagcgaa ccgtttgtga tccaggacct cgacggggat cccatgtttt
     3181 ttggcgcgca cgaggccgta ggcgtcggcg cgattgctga gcacgacctc gattttcgcc
     3241 tctagctctt tcccctcgat ggcatcgatg atggattgaa gattagtgcc gctgccggaa
     3301 atcaatacgc cgatgggaac ttgtcgtcgc atggcttgtc cctcaggaaa caaaagtaac
     3361 ctctcgccgg ccttttttga tctcgccgat catgaagtgc cgctcgcgca ttttcttgag
     3421 catcgacata acgtcgtcca cctgccgctt gccgataacc aggatcatgc ccaggccgtt
     3481 attgaacgtc cggtccattt catctcgtga aacgcgtccg atcttttgga tcaaatcaaa
     3541 caccggcgga accggccaag tgcgccggtc tatccaagcg cgccgtcctt caggcaaaat
     3601 tctcggcaag ttgcccggaa tacctccgcc cgtaatatgg gctgcgcctt tgattggata
     3661 ttttgcgaat aggccccgag tcatcctggc atagatccgc gtcggctcta acaactcctc
     3721 acctaaactc ctccccaact cgggcacctg ttgccggagt ttcagccgac ccacatcaag
     3781 caagaccttt ctcgccaacg agtagccatt gctatgaaga ccgctggagg gaagcccgat
     3841 cagaacatct cccggcacaa tcgacttgga attgagaact ctcttccttt cgatgactcc
     3901 gactacaaag ccagccagat catattcacc gttgggaaaa tcaccggaat gctctgcggt
     3961 ctcgcccccg atcaagacac agcccgcctg acgacatccc gcagcaatgc ccccgacaac
     4021 aacttcagcg acatcgacgt caagcttgcc acagacgaaa tagtccaaga agaaaagcgg
     4081 ctcggcgcct tgagtgatga tatcattcac gcccatggcc acaagatcga tgccgacggt
     4141 gtcgtgtttc ttcatcatga acgccacttt gagcttggtt ccgacgccgt ccgtggaaga
     4201 aaccagcacc ggatggcgat agcctcgact ctttagatcg aagagcgcgc tgaatcctcc
     4261 gacacctgcg agcactcccg gcttcttggt tcttgatgcg atgggaccga tccggcgcac
     4321 tagttcgtcg cctgcttcga tactgacgcc ggccttggcg taggtcatgt ttccggaggg
     4381 tgccattcga aaggtgaaat gcctgcgagt gttaactgaa tggagatcga aattacaagt
     4441 agtatagcct acgaacgaaa caaattcatg cggtggaccc cgggatagca gcgggaatac
     4501 cgtcaacacc ctgaagcagg gcgcgtatct tcagagtgta ttcgccaaat tgcgtcgtac
     4561 tcatcatctc catcgttctc gggcgtggca attggatcgc gatctgctcc aggagcctgc
     4621 ccggtcgggc cgacatcaca atgacgtggt ccgacagaaa caccgcttcc tgaatgctgt
     4681 gggtcacgaa caggacggtc ttccttctat cctgccaaat acgcagcaac tcgatatcca
     4741 actcgtcgcg cgtcatcgcg tcaagcgctc cgaagggttc atccatgagc agcagctgcg
     4801 ggtccatgac caacgcgcgg caaagggaga ctcgctgctg catgccgccg gaaagctgat
     4861 ggggatacat ttcctcgaaa ccttccaagc ccaccagctt gagcaatgag cgagcccgtt
     4921 ccgtgtggct ggccaggtcc agtttcgcga attccaccgg caaaaggacg ttgcccaaca
     4981 ccggccgcca ctttaagaga accggcgatt gaaataccat cccgacattt tcaagaggct
     5041 catcaaccgg tttgtcactc acggcaacgc ttccggagga agcgggaagc aaccctgaaa
     5101 cgattttaag caaggtgctt ttgccgcaac cgctgggacc caccagagag acaaaatcgc
     5161 ccgcctggat ctgaaatgaa acattttcga gggcatgaac ggtcttcgcg ccactcgtaa
     5221 aaactctcga aacgttctga acggcaatca gcagcacgtc gagatctcgg aactctcctc
     5281 tggttactgc ttgatgaatc ggttggtgaa aacctccgcc gccgtcacct ttcgccccac
     5341 atcgaaatac tcattcgtaa tccggaccgt tttatccatg atatctggtt tcatgaagcc
     5401 caagcccgac tgcacggact ccggcggaat gaatacttcg tacagcgcgt ttttaagctg
     5461 tagggtcgtc aaagccaagt ccaattcagg tttttgtttg agcaagatct gcagggcctc
     5521 ttcctgatgg tctcgggtat agcgcagtcc ctccatcgtt ccttcaacat aggctctaac
     5581 caagtccggc cgctgcttga tatcatcttc tttgacgatc aagccggtgc cgtagatatc
     5641 gaggcccgtg tctttcatga agattctatt cagcttcata ccttgtttcg ttgcggccaa
     5701 ctccgccacc tcatcattcg aggcacggta catgacgatg aagtcagtcc gcttcgccac
     5761 cagcgcggca ggttgaatcg gcgccgcggt ttcttggatc ttgatagatt tgaaatcgat
     5821 cttgttgatc cggcagtagg ccggcatcaa gtaccactga gcctgccccg gactgatcgc
     5881 ccaagacttg ccttccagat ctttcagctt tttgatcgcc gtatcctcgc gccacatgat
     5941 ggtcgcggga acatagccga actgtgccac tgtcagcacg ggcgttccgc gcgttcgagc
     6001 gacaatcgcg gtggacatgt cggcgaatcc gaagtcggag cctcccgcag cgatggcccg
     6061 cactgtgtcc gcagacccac tgcccgcctg aatcgtcgcc gtcaagcctc tttttgcgta
     6121 aaagcctttt tccagggcca caaaccaggg agcatgcttt ccgccgatga taaaatcgag
     6181 acgtatcgtt cctgtcttgg tctcggcggc gtcggaacca acatgggcaa aagaaatcaa
     6241 cacaacgacc actactgcaa ttattgcggg agaccagagg ggaaagtgcg tcttcagcaa
     6301 tgccatcatt atcctccgta aatattttca gaacgaacct tcgacctgac gaacttcagc
     6361 gctccaggga gcgatttttc tttgcacgat ctcgaccagc cagaaaagac ccaaggacag
     6421 aacggccaaa ataagcagga ccaccagcgc aaaggccgta tccagactat aatttgcctg
     6481 cagcacgaga tagccgagcc cttcgttggc ggcaacaaac tccgccacga tggcgcccgt
     6541 cacggagaga acgatcgcga ctttgattcc ggcgaacata aacggtatcg catttgggag
     6601 ccggatcttc ttgaagacat cgaactgcga cccgcggtta attcgcgcca attccaggag
     6661 ctccggctcc acttctttaa gccccacgac ggtattcacc acgacgggaa aaaacgccac
     6721 aagaaaagca atcgcgatct tagacatgag tccatatccc acccagatca ccatgagcgg
     6781 cgcaaaggcg cttttcggca tggcatagag aatcacgatg agcgggtaaa tcgtattctg
     6841 cagatggcgc gagtaaacca cggcaatcgc gaatgtcacc ccgagaacca aagagaaggt
     6901 aaacccaccc accgcttcgg tgaacgtaat gagcgagtga ttgcccagga tgggtaattt
     6961 ctttatgaaa gacatccaca aatctcgcgg cggcggcaag atgaaagagg gaaccttgat
     7021 cacctcgacc aacatctccc acagaatcaa gagcacggcg aaaaagatca gcggctcgat
     7081 aacgttctta acggacggtt tcatgacttt ggctcccgga gccgacgtta gaagaaagct
     7141 atgaacctgt caaggaaacg gcggaagacg ggaaacggcg gaagacggga aacggcggaa
     7201 g
//
LOCUS       DASSWZ010000176         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_274775, whole genome shotgun sequence.
ACCESSION   DASSWZ010000176 DASSWZ010000000
VERSION     DASSWZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274775"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFN58_04340"
     CDS             <1..720
                     /locus_tag="VFN58_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET8917253.1"
                     /translation="YEQGRRSVYQLVIKELRAGRQAFIVYPLVEASEELTQVRDASQM
                     AEKMRQGVFKDFGVGLVHGKMSTAERDDVMRSFRDGAIGVLVATTVIEVGIDIPNATV
                     MVIEHAERFGLSQLHQLRGRVGRGSAAGYCLLINRAPRTPLAGKRLRVMEQQHDGFKI
                     AEADLAMRGPGEFLGTRQSGLGDFRLANLVRDAKILMEARKEAQAWLEKDPELSSLES
                     AELREVLLHRWGQRLQLGAVG"
     gene            954..2075
                     /locus_tag="VFN58_04345"
     CDS             954..2075
                     /locus_tag="VFN58_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8917254.1"
                     /translation="MKMEARRRGEDIVDFGMGNPDLPTPPHVVAKLVEAATKPQNHRY
                     SVSRGIYKLRVAICDWYKRRYQVGLDPDSEAIVTIGAKEGLSHLAWAIVDPGDVVLCP
                     SPTYPIHQFAMILAGGDLRCIPLISSEGFLGHLEEAVKQTWPKPKLLIISFPHNPTTE
                     VVELDFFERIVKFAQENELIVIHDLAYADLTFDGYQAPSLLQVRGAKEIGVEFFSLSK
                     SYNMPGWRVGFAVGNRDIIHALARIKSYLDYGIFQPIQIAAIHALNGPQDCVEGIRLM
                     YQKRRDSLVDSLDRAGWQIAKPKGTMFVWAEIPEPAKKMGSVEFSKFLLQEAKVAVSP
                     GIGFGQYGDDHVRFALIENEHRTKQAVRNIRRALGKLLD"
     gene            2247..>2994
                     /locus_tag="VFN58_04350"
     CDS             2247..>2994
                     /locus_tag="VFN58_04350"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET8917255.1"
                     /translation="MASAKVRHKVGLGLLGSGVVGEAIQDIVFNDLRGQVGSDLELEI
                     RKIYTRRPEKKKWFSKHPSLFTTKAEEVIDDPGIGIVVEVLGFQHEAELPVFRDYILR
                     ALERGRSVVTSDKAVLARFGKELWAAAKKHGRQLRFEACVGGGIPIIRSLTESFAVEE
                     PEAIFGIVNGTCNYILSQMEQSGKPYADALKEAQQKGYAETNPTADVSGSDAEAKLLL
                     LSAVGFGLQLQPGTTWRKGIEDIHAVDFRYA"
BASE COUNT          677 a          759 c          889 g          669 t
ORIGIN      
        1 tacgagcaag gccgtcggtc ggtctaccaa ttggtcatca aagaactgcg cgcggggcgt
       61 caagccttta tcgtctatcc cctagtcgag gcctccgaag agctcactca ggtgcgcgac
      121 gcgtcgcaaa tggcggaaaa gatgcgtcaa ggcgttttca aagacttcgg cgtcggtctt
      181 gtgcatggca aaatgtccac ggcggagcga gacgacgtca tgcgctcctt cagagacggc
      241 gccatcggcg tgctcgtcgc caccactgtc atcgaggtcg gcatcgatat tcctaatgcc
      301 acggtgatgg ttatcgagca cgccgagcgc ttcggcctct cacagctgca tcagcttcgg
      361 ggccgagtgg gtagagggag tgccgcagga tactgtttgc taatcaatcg cgcgccgcgc
      421 actccgctgg cggggaagag actgcgtgtc atggagcagc agcacgacgg tttcaagatc
      481 gctgaagctg atcttgccat gcgcggaccg ggtgaattct taggtacgcg ccaatccggt
      541 ttaggagatt tccggctggc taatctcgtc cgcgatgcca agattttgat ggaagcgcgc
      601 aaggaagccc aagcctggct tgagaaggat cccgagctga gcagcttgga atcggcagag
      661 ctgcgagaag tcttactgca tcgttggggc cagcgactac aactgggagc ggtaggctaa
      721 cccggactaa tcatggtctg aggaaaaact acgacaccgt ttggatcgag cggcagccct
      781 gaacaatccg ggccagcggt gtattgactg gattttctca agttgcaccc ttcgctacca
      841 tcgcttataa aagtagttta gaatgcactg cttaaagagc atgaggtaag actttcgtgg
      901 aatttcaacg aatcaagcgg ctgccgccct atgtcttcag catcatcgac ggcatgaaaa
      961 tggaggcgcg tcgccggggc gaagatatcg tcgacttcgg catgggtaat ccggatctgc
     1021 caacgccgcc tcacgttgtc gccaagctgg tcgaagcggc aaccaaaccg caaaatcatc
     1081 gctactccgt ctccaggggc atttacaagc tccgtgttgc gatctgcgat tggtacaagc
     1141 ggcgctatca ggtgggcctt gatcccgata gtgaggcgat cgtcaccatt ggtgccaaag
     1201 aagggttgtc gcatttggcg tgggcgattg tggaccccgg agacgttgtg ctgtgtccca
     1261 gcccgacata tccgatccat caatttgcca tgatcttggc gggcggcgat cttcgttgta
     1321 ttccgctcat tagcagcgag ggatttttgg ggcacctcga agaagcagtg aagcaaacgt
     1381 ggccgaagcc caaactgctt atcatcagtt ttcctcacaa tccaaccacg gaagtagtgg
     1441 agctggattt cttcgagcgt attgtcaagt ttgcacagga aaatgagctg atcgtcattc
     1501 acgacttggc gtatgcagat ttgaccttcg acggctacca ggcgccgagc ctgcttcagg
     1561 ttcggggcgc taaagaaatc ggcgtggaat ttttttcatt gtcgaaaagt tacaacatgc
     1621 ctgggtggcg ggtggggttt gcggtgggta atcgggatat tatccacgcc ctggcgcgaa
     1681 tcaagagtta cctcgactat gggatttttc agccgatcca gatcgccgcg attcacgcct
     1741 tgaatgggcc ccaagactgc gtcgagggga tccggctgat gtatcaaaag cgcagggatt
     1801 cgttggttga tagtcttgac cgggcaggtt ggcaaatagc caaaccgaag ggcacgatgt
     1861 ttgtgtgggc ggaaatcccg gagccggcga agaagatggg gtcggtagaa ttttccaagt
     1921 tcttgcttca ggaagctaaa gtagcggtgt cccccggcat cggtttcggc cagtacgggg
     1981 acgatcacgt ccgctttgcc ttaatcgaaa acgaacatcg taccaagcaa gcggtacgca
     2041 atatccgccg cgcgttgggc aagttgctcg actgaagctc gctcgcgtct aattcgcgct
     2101 tcccatttct gggctcccgc ctgcgcggga gtgacgagag tttttagttt cttagcgagg
     2161 aattttcacc atgccatcgg ccctgctagc cgatttcatg gttccatagg tctcaaacta
     2221 agctctgggt tctgttggat aagatcatgg catcggcaaa ggtgcggcac aaagtcggtc
     2281 tcggtctgct cggttctgga gtggtcggcg aggcgattca ggatatcgtc tttaacgacc
     2341 ttagagggca ggtgggaagc gatctggagt tggagattcg taagatctac acccgcagac
     2401 cggagaaaaa aaagtggttt tccaaacacc cgtcgttatt cacgacgaaa gcggaagaag
     2461 ttatcgacga tcccggcatc gggattgttg tcgaagttct agggtttcag cacgaggcgg
     2521 agcttcccgt atttcgagac tatatcctgc gtgctttaga gcgggggaga tccgtcgtca
     2581 cgtcggacaa ggcggtgttg gccaggttcg gcaaagaatt atgggcggcg gctaaaaagc
     2641 atggcagaca actgcgcttc gaagcgtgcg tgggcggcgg catccctatc atacggtcac
     2701 tgaccgaaag ctttgcggtt gaagagcccg aagccatatt tggtatcgtt aacggcacct
     2761 gcaactacat cctttcacaa atggaacaaa gcggtaagcc ctatgccgat gcgctcaaag
     2821 aagcacagca gaagggctac gccgaaacta atccgacggc ggatgtcagc ggcagcgacg
     2881 cagaagccaa actactgctt ttaagcgccg tcggctttgg cctacagctt cagcccggaa
     2941 cgacctggcg caaggggatt gaagatattc acgcggtgga ttttcgctat gctg
//
LOCUS       DASSWZ010000177         9266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_444328, whole genome shotgun sequence.
ACCESSION   DASSWZ010000177 DASSWZ010000000
VERSION     DASSWZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444328"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /gene="mraZ"
                     /locus_tag="VFN58_04355"
     CDS             <1..466
                     /gene="mraZ"
                     /locus_tag="VFN58_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET8917256.1"
                     /translation="LRRRMFRGSFEHTLDGKGRLSIPVKFREVLFGKSDERIVITNFV
                     VDGMKCLDVYPLDEWSRLEEEIRRKPKFDRKMLMFQNYYLGGATECVVDKQGRILIPP
                     LLRKYAELKRDVVLVSALEKFRVWDLEAWKKIFSDAEEKLMQDPDFLGDLGL"
     gene            514..876
                     /locus_tag="VFN58_04360"
     CDS             514..876
                     /locus_tag="VFN58_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET8917257.1"
                     /translation="MLQRKPIQDISVLDVAGDIDFREMIRIKDLIGGLIEKDRTKVVL
                     NLRAVDHINYLSIGVLLERLKLLRNLNGDLKLAGMSPFLRDIFKVVGMDRFFEDYTSL
                     EDAIESFDDDWEGDGTSH"
     gene            877..1806
                     /gene="rsmH"
                     /locus_tag="VFN58_04365"
     CDS             877..1806
                     /gene="rsmH"
                     /locus_tag="VFN58_04365"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET8917258.1"
                     /translation="MVFMHVSVMAREVIDFLRPEPRKRYLDGTLGGGGHTEQILIHSG
                     PDGRVLGLDLDDEALAAVGQRLSGFGDRLMVRQANFAAAGAILSEIGWPLVDGIVLDL
                     GISSHQLESRERGFSFRGGARLDMRMDRHQALDAYQIVNAFSVSELERILREYGEEPQ
                     ARRIALAIESERKMKAIKTTEELAEIVARVKGKGGRDHHPATQTFQALRIAVNQELEN
                     VDRFLEKGYELLQPKGRLVVISFHSLEDRLVKTAFRKWNRDCICPPRTPTCQCGWSRK
                     VKLLTTRPLSPSPGEIETNPRARSAKLRAVERI"
     gene            1917..2165
                     /locus_tag="VFN58_04370"
     CDS             1917..2165
                     /locus_tag="VFN58_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET8917259.1"
                     /translation="MIAISALGLVGVALLHVWLRLQVVHMGYVLSTTSKLQNQLEQEN
                     RELKVEFATLTSPDRLEAMSRRRLGLRPPEKGQVIVLP"
     gene            2162..4111
                     /locus_tag="VFN58_04375"
     CDS             2162..4111
                     /locus_tag="VFN58_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET8917260.1"
                     /translation="MKRSVQGNRLRLGVAKVFFLVLFVVAAGRAFQLQVLQGERLKRL
                     GERQHLKEWIVLPKRGAVMDRAGEPLALSLEAQSVYARPHRIHDPGSVSLALAKILNL
                     NVVEVKQKMTSDKPFVWIKRQIGPQEAAQIQALSIDGIGMFYEPNRYYPQGQLAGQVI
                     GFVGRDSEGLEGLELHYNGYIRGETGSSVTERDALGRRVLVQGVEGLQIPPGGDIHLT
                     LDTSIQHLAEKELEATISKYRAKAGTAIVVEPFTGEVLALANYPSFNPNNFSKQSSQQ
                     KRNRAIADSFEPGSTFKTILAAAALEEGVVGKDDLFYCEMGKYAYAGKLIHDTHPYGW
                     LPFSKILQVSSNIGFTKVAEKLKRERYFKYIEKFGFGQITGIDVPGEVPGLLRKPESW
                     SAIDLATHAFGQGISATPMQMVMAYAAIANGGFLMRPYLVRRVVGSKGEVLLENQPHV
                     VRRVVSEKTSRLLASMLKEVTNEGGTGVMAKVEGFEVAGKTGTAQKADLTHGGYAAKR
                     VGSFVGFVPADAPKLVALVLVDEPEVNVYGGVVAAPVFRNIARGALRHLAVAPQKPDL
                     VPATVGQAELPVRQVLKKEIEVADGNAAAVPDFVGLSLREAVEKARAMRVKVKMRGNG
                     YVVRQSPAPGGRWSEESILVLSLQG"
     gene            4116..5639
                     /locus_tag="VFN58_04380"
     CDS             4116..5639
                     /locus_tag="VFN58_04380"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET8917261.1"
                     /translation="MRLREFLALEQVEEAEGNLDQEVTGLTYDSRRLRAGEIFFAIPG
                     EKVDGHEFIPQVLQTGAAGVVVARKGSWPQGTTWIRTKEVRRTMGLWGAYFYGRPSRR
                     MKLVGITGTNGKTTVSYLVESILSAAGLEPGVIGTIDYRYHGRHVPSHHTTPESLELQ
                     SLLAEMETAGVQSVAMEVSSHALVQERVRGIDFDVALFTNLSRDHLDYHASMDDYFLA
                     KSKLFTDYLKVSVKAKKAAVIYGGDARGSELLLKVHDAGLEAWSYGEDNEWDIHPVKV
                     QSDVAGLRGQLKFKGRYLDFSSPLIGAANLQNIMGAVGVAGCLELPTDVIAKGIEQLA
                     SVPGRLEKVENNLKVSVLVDYAHTPDALEKVLGAVRPLTQRRVLTVFGCGGDRDRGKR
                     PLMGEIAARLSDLLVLTSDNPRTEDPLGILAEIEDGIRKSGLKRLGDSSLQTERGYWV
                     EKDRRAAIRIALRAAQRGDLVLIAGKGHEDYQILGAKKIHFDDREVAREELSRIGSS"
     gene            5650..7053
                     /gene="murF"
                     /locus_tag="VFN58_04385"
     CDS             5650..7053
                     /gene="murF"
                     /locus_tag="VFN58_04385"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET8917262.1"
                     /translation="MGWRRKEILLATGGRLLRGGKSDRLGEVVTDSSKVKRGSVFVAL
                     KGERLDGHRFVRDAVRRGASCVLVHRDLHPSAYGAATVVKVRDTLRALGDLAHYRREI
                     VGPKVLAITGSNGKTTTKEMVAAILEGAYVNKKPLRGRVLKTEGNFNNLVGLPLTLLR
                     LHKSNKVVVVELGTNRPGEIKRLTEIAAPDVGIITAVAAAHLEGLNSLAGVAREKGAL
                     FGGIRPTGMIAVNLGDPWVRRLGEKFRGKKITYGEGGEVRAESWRSLGAKGTMLYLRT
                     GRHRSRVRLNFLGEHNVVNATGAAAMAYGFGASLAAIRKGLQKTKPFPMRMQLQNWRK
                     IGIINDAYNANPASMEAAVKTLAGIACSGERIAILGDMFELGRQSRQQHLQLGKQVAR
                     AGIDRLYLLGAQAGHVRQGALLGGMAAQRIVIGRGHAEVAKLLRSHVSKGDWLLFKGS
                     RGMKMEMVLNKFKSAKA"
     gene            7064..8140
                     /gene="mraY"
                     /locus_tag="VFN58_04390"
     CDS             7064..8140
                     /gene="mraY"
                     /locus_tag="VFN58_04390"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET8917263.1"
                     /translation="MLSHLLFQLHTTYSGFNVFRYITFRAAMAALMALVISFLLGPWL
                     IRSLTAKQMGQQIREDGPASHSTKAGTPTMGGVVIILALSLSTLLLADIRNPYILLAL
                     FATVGIGAVGLIDDYLKLSRKNSKGLPPRAKLLGQFAVAFVTAAILCVYLKFNTELGI
                     PFVKNIRPDLGLWYIPFAMFVIVGASNAVNLTDGLDGLAIGPVMITAGTYAVIAYVTG
                     HLKLAEYLQIPYVAGVGELSVFCAAVVAAGLGFLWYNAYPAQMFMGDVGSLSLGTALG
                     IVAVMTKNEILLIIVGGVFVMEALSVIFQVVSYKLRRKRVFLMAPIHHHYELKGWPEP
                     QIIVRFWIISFICAVVGLSTLKLR"
     gene            8156..>9266
                     /gene="murD"
                     /locus_tag="VFN58_04395"
     CDS             8156..>9266
                     /gene="murD"
                     /locus_tag="VFN58_04395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET8917264.1"
                     /translation="MELRDKQVLVVGLARTGIECARFLVNQGAKVLVSDLRSETDLEQ
                     AIHTLKGLPIDYLLAGEERRWLEGMDMVVPSPGVPPTNPLLQEATSRGIEILSEIEVA
                     YRFCRFPIVAVTGTNGKSTTTTLVGEMLKANGTKVFTGGNIGAPLIGFVGGDWEWGIV
                     EISSFQLEWIEEFRPRVAVLLNLSEDHLDRYPDFAAYCRAKERILENQTAKDVAVLNR
                     DDPLVWGLRQGRRARIISFGFSEVDEGVFAKSEEIVWRDGSSEERFPLGHVKIQGVHN
                     VENMMAAIATAKAIGIPAQIIQQTLEEFPGLEHRLEFVREQDGVRYYNDSKGTNVGAV
                     VKSLASFSTPVILLAGGVDKGGDYRVLRDGVGKRVK"
BASE COUNT         2063 a         2206 c         2893 g         2104 t
ORIGIN      
        1 acttaggcga cgcatgtttc gtggcagctt tgaacatacg ctcgatggca agggacgttt
       61 gagcattcct gtgaagttcc gggaggttct tttcgggaaa agtgatgagc gcatcgtgat
      121 tacgaatttc gttgtggatg gaatgaaatg ccttgacgtc tatcccctcg atgaatggtc
      181 acggttggaa gaagaaattc gaagaaagcc aaagttcgat cgcaaaatgc tgatgttcca
      241 gaactattat ctcggcggcg cgactgaatg cgtcgtcgat aagcaaggcc gtattttgat
      301 tccgccgctc ttgaggaaat acgccgaact gaagcgagat gtcgttctgg tctcagcttt
      361 agaaaagttt cgcgtctggg atctcgaggc atggaaaaaa atattctcgg atgctgaaga
      421 aaagttgatg caagacccag attttctcgg tgacctggga ttgtgagaac gtcgaagaaa
      481 aatgcacgtg gttagatgaa aggagaatgg ggaatgctac agcgaaaacc catccaggac
      541 atttcggtgc tcgatgtggc gggcgatatc gattttcggg agatgatccg gattaaagac
      601 ttgatcggtg gactgattga aaaagaccgc acgaaagtcg tcctgaatct tagagccgtg
      661 gatcatatca attatttaag catcggagtt ctccttgaac ggctcaagct gcttagaaat
      721 ttgaacggtg atctcaagct ggccgggatg agcccgttcc ttcgcgatat ttttaaagtc
      781 gtcggaatgg atcgcttctt cgaggactac acctcgttag aagatgccat cgagagcttt
      841 gacgacgact gggaggggga cggcaccagt cactaaatgg tgttcatgca tgtttctgta
      901 atggctcggg aggtcattga cttcctcaga cccgaaccgc ggaaacgata cctggacggc
      961 accttgggag gcggaggaca cacagagcaa attttgattc acagcggccc cgatggacga
     1021 gtcctcgggc tggatttgga tgacgaggcg ttggcggcgg tgggccaacg gctgagcgga
     1081 tttggcgatc ggctgatggt gcggcaggcg aatttcgcgg cggctggggc gatcttgtct
     1141 gagatcggtt ggccgctggt ggatggcatt gttttggatt tgggtatctc gtctcatcag
     1201 ttggaatctc gtgagcgagg cttcagtttt cggggcggag cgaggctcga tatgcgaatg
     1261 gatcggcatc aagcgctgga tgcgtatcag atcgttaacg cgttttcggt atccgagcta
     1321 gaacgaatcc ttcgtgagta tggcgaagag ccgcaggctc gccgtattgc gctagccatc
     1381 gagtctgaac ggaagatgaa ggcgatcaaa acgacggaag agcttgctga aattgtcgca
     1441 cgagttaagg gtaagggtgg acgggatcat catccggcga ctcaaacctt tcaagctctg
     1501 agaatcgcgg tcaaccaaga gttagaaaat gtggatcggt ttttagagaa gggttacgaa
     1561 ctgcttcagc cgaagggtcg gttggtggtt atttcgtttc attcgttgga agatcgactg
     1621 gtgaaaaccg catttcggaa gtggaaccgt gattgtatct gtcctccaag gacgccgaca
     1681 tgccagtgcg gttggagccg taaggtcaaa ttgcttacga ccaggccgct ttctccttca
     1741 cccggcgaga ttgaaaccaa cccacgcgcc cgctcggcca agttgcgcgc agtggaacgg
     1801 atctagggga atcacgagca gtggcaaccg cggcaagaca gagcgtcaaa acctcgcgat
     1861 ctcaaaggga gcgggtcgag agcgccggtc ggcgtcagcg cccgcggcag gtcatattga
     1921 ttgccatatc cgccttgggt ttggttggag ttgcgttgct gcacgtttgg ctgagattgc
     1981 aggtggtcca tatgggctat gtcctttcga ccacgagcaa gctccagaat cagcttgaac
     2041 aggaaaatcg agaattgaaa gttgaattcg ccacgttgac ctcgccggat cgtttggaag
     2101 cgatgtctcg ccggcgcttg gggctaagac ctccggagaa gggtcaagtg attgtcttgc
     2161 catgaaacgc tccgttcaag gaaatcgttt gcgtttgggt gtcgccaagg tatttttctt
     2221 ggttcttttt gttgtggcgg ccggacgggc atttcagctt caagttctgc agggggaaag
     2281 gctcaaacga ctcggtgaaa ggcagcacct caaggagtgg atcgtgcttc caaaaagagg
     2341 tgcggtcatg gatcgtgcgg gtgagccgct ggcgttgagt ttggaagcac agtcggttta
     2401 cgctcgccct catcgaattc atgatccggg aagcgtgagc ttggccctcg ctaaaatcct
     2461 caatctcaac gttgtcgaag tgaagcaaaa aatgacctcg gacaagcctt ttgtttggat
     2521 caagcggcaa atcggtcctc aagaagctgc acagatacaa gccttgagta tcgatggcat
     2581 cggcatgttt tatgagccga atcgctacta cccacagggt cagttagcgg ggcaagtgat
     2641 cggatttgtc ggtcgcgatt cggaaggatt ggaagggttg gagttgcact acaatggcta
     2701 catccgggga gaaaccggct cgtcggtcac cgagcgtgac gctttaggcc gcagggtttt
     2761 ggtccaaggc gtcgagggcc tacagattcc accgggcggc gacattcatt taactctgga
     2821 tacgtcgatt cagcaccttg cagagaaaga attggaagca acgatcagca agtaccgagc
     2881 aaaggcgggg actgcgatcg tcgttgagcc tttcacgggg gaggtgctcg ccctggcgaa
     2941 ttatccgtcg ttcaacccga acaatttttc caaacagtcg tcacagcaaa agcgcaatcg
     3001 cgccatcgcc gacagcttcg agcccggctc gacgtttaaa accatccttg cggccgcggc
     3061 cttggaagaa ggcgtggtgg gtaaagacga tcttttctat tgtgaaatgg gaaaatacgc
     3121 ttatgccggg aagttgattc atgacacaca cccgtacggt tggttgcctt tctctaagat
     3181 ccttcaagtc tcgagcaaca ttggattcac caaggtagca gagaagctca agcgggaacg
     3241 atattttaag tacattgaaa aattcggctt cggtcagatt accggcattg atgtgcccgg
     3301 cgaagttccc gggctgcttc gaaaaccaga atcctggtcg gcgatcgatc tcgccaccca
     3361 cgctttcggc cagggcattt cggcgacgcc gatgcagatg gtgatggcct acgcggctat
     3421 cgcgaacggt ggctttctca tgcgtcctta cttggtacgg cgcgttgtgg gttcgaaagg
     3481 cgaggtgctt ctcgaaaacc aaccccacgt ggtccggcgt gtggtctcgg agaagacctc
     3541 gcgattgttg gcgtcaatgc taaaggaagt taccaatgaa ggcggtaccg gggttatggc
     3601 gaaggtagaa ggattcgagg tggcgggaaa gacgggcacg gcgcaaaagg cggatcttac
     3661 ccacggcggc tatgccgcga aacgggtagg gtcgtttgtc gggtttgtcc ctgcggatgc
     3721 ccccaaattg gttgctttgg tcctcgtcga tgagccggaa gtcaatgtct acggcggcgt
     3781 cgttgccgct ccggttttcc gcaacatcgc gcgcggggcg ctccgtcacc tggcggtggc
     3841 tccgcaaaag ccagacttgg ttccggcaac ggttggccaa gccgaacttc ccgtccggca
     3901 agttctgaag aaggaaatcg aagtcgccga tggcaacgcc gcggcggtac cggacttcgt
     3961 tggcctcagt ctccgggagg cggtggagaa ggctcgagct atgagggtca aggtcaaaat
     4021 gcgcggaaac ggctatgtgg tcagacagtc cccagcgccg ggaggtcgct ggagcgagga
     4081 aagcattttg gttttaagcc tccagggtta gtgaaatgcg tctgcgggag tttttggcgc
     4141 tagagcaggt agaagaagcc gagggaaatc tcgatcaaga agtgacaggg ctaacctacg
     4201 attctcgccg attgagagcg ggagaaatct tctttgccat tcctggagag aaggtcgatg
     4261 gccacgagtt cattcctcaa gtacttcaga ctggcgccgc cggggtcgtc gtggcgcgca
     4321 aggggagttg gccgcaagga acaacctgga taaggacaaa agaggttcgg cgcaccatgg
     4381 gattatgggg ggcgtatttc tacggacggc cgagccggcg gatgaagctc gtgggtatca
     4441 ccgggacgaa tggaaagacc accgtgagct atctggttga atcgatactt tcggccgctg
     4501 gcttggagcc gggcgtgatc ggcacaatcg actatcgtta ccatggacgc cacgttccgt
     4561 cgcatcacac gacgccggaa tcgcttgaat tgcagtcgct cttggcggag atggaaacag
     4621 ccggcgttca gtcagtcgcg atggaggttt cctcccatgc tctggttcaa gagagggtgc
     4681 gaggaatcga ctttgacgtt gcgctattca cgaatctctc ccgggatcat cttgattacc
     4741 atgcgagcat ggacgactat tttttggcta agagcaagct ctttacggat tatctgaagg
     4801 tgagtgtgaa ggcaaaaaag gctgccgtca tttacggagg agacgccaga ggaagcgagc
     4861 ttttactcaa ggtccatgac gcgggcttgg aggcttggag ctacggcgag gacaacgagt
     4921 gggacattca ccctgtcaag gttcaaagtg acgtggctgg gcttagagga caattaaagt
     4981 ttaagggtcg gtacctcgat ttttcctcgc cgctcatcgg cgcggccaat ttgcaaaaca
     5041 tcatgggcgc cgtcggagtc gcgggttgcc tggaactgcc gaccgacgtg attgccaagg
     5101 gtattgaaca gctggcatcg gtgcccggga gattggagaa ggtggaaaac aatttgaaag
     5161 tttccgtctt ggtggactat gcccacacgc ctgacgcgct cgaaaaagtg cttggtgcgg
     5221 ttcgtccatt gactcaacgc agagtcttga cggttttcgg ttgcggtggg gaccgggatc
     5281 ggggaaagag gccgttgatg ggcgagattg ccgcgcggtt aagtgatctt ttggttttga
     5341 cttcggataa tccgcgtacc gaagatccac tcgggattct ggccgaaatc gaggacggaa
     5401 taaggaagtc cggtttgaag aggttggggg atagtagcct gcaaacggaa cgcggctatt
     5461 gggttgaaaa agaccggcgc gcggctattc gcattgcgtt gcgggcggct caacggggtg
     5521 atttggtctt gatcgcgggt aaagggcatg aggactatca gatcctcgga gcgaagaaaa
     5581 ttcacttcga tgatcgagaa gttgcacggg aggagctgag tcggatcggc agctcatgat
     5641 ggttagggaa tggggtggcg ccgtaaagaa attctgctgg cgactggggg aaggctcctg
     5701 cgcggtggaa aaagcgatcg gctcggtgaa gtggtgacag attcgagcaa agtaaaacgg
     5761 ggatcggttt ttgtcgcgct caagggcgag cggttggacg gccatcgatt cgtgcgcgat
     5821 gccgtgcggc gcggcgcgag ttgcgtcctc gttcatcgcg atcttcatcc gtcagcgtac
     5881 ggcgctgcca cggtggtcaa ggtgcgcgac acgctaagag cgctcggcga tttggctcat
     5941 tatcgccgcg agatcgtcgg cccgaaagta ttagccatta ccggatccaa tggcaagacc
     6001 actaccaaag aaatggttgc ggcgattctc gaaggagctt acgtgaataa aaagccgctt
     6061 cgagggaggg ttttgaaaac cgagggtaac ttcaacaatc tcgtcggcct gcccttgaca
     6121 ctccttcgtc tgcacaaaag caacaaggtg gtggtggtgg agcttggaac gaatcgtcca
     6181 ggggagatca aacgcctcac cgagatcgcc gctccggacg tgggaattat caccgccgtg
     6241 gctgcggccc acctcgaggg gctgaacagt ttggcggggg tcgcccgtga gaagggggca
     6301 ctcttcggtg ggattcggcc caccgggatg attgcggtta acctgggcga cccgtgggtg
     6361 cgccgcctcg gcgaaaaatt ccgaggcaaa aagataactt atggcgaggg cggtgaggtg
     6421 cgcgccgaat cgtggcgctc cctcggcgct aaagggacga tgctttatct ccgcacgggt
     6481 cgtcatcgat cccgcgtccg gctcaatttt ctcggcgaac acaacgttgt gaatgccaca
     6541 ggggcagcgg cgatggctta cggtttcggc gctagtctgg cggctattcg caaaggtttg
     6601 cagaagacaa aaccattccc gatgcgcatg cagctccaga actggaggaa aataggaatt
     6661 atcaatgatg cctataatgc caatccagcc tccatggaag cggccgtcaa gacgctagcc
     6721 gggatcgcat gcagcggcga gagaatcgcc atacttggcg acatgtttga attgggccgc
     6781 caaagccgcc aacagcatct gcaattgggc aaacaggtgg cgcgcgccgg tatcgatcgt
     6841 ctgtacctat tgggcgcgca agctgggcac gtaaggcagg gcgcgcttct gggtggcatg
     6901 gcggcgcagc gaatcgttat cgggagaggt catgccgagg ttgcaaagct gctgagaagt
     6961 catgtgagca aaggggattg gctacttttc aagggctcac gcggcatgaa gatggaaatg
     7021 gtcctgaaca aatttaagag cgcgaaggct taggagaatc gacttgctgt cccatctgct
     7081 cttccagctc cacacgactt attccggctt caacgtgttc cgctacatca cgtttcgcgc
     7141 cgccatggca gcgctgatgg cattggtaat ttcgtttctg ctgggaccct ggttgatccg
     7201 ttcgctgact gctaaacaga tggggcagca aattcgtgaa gacggaccgg cgtcccattc
     7261 gaccaaagct ggcaccccga caatgggagg agttgtcatc attctggcgc tgtcgttgtc
     7321 gacgctctta ctggctgaca tcagaaaccc gtatatcttg ctcgcgttgt ttgcgacagt
     7381 cggcatcggc gcggtgggtc ttatcgatga ttacctgaag ctcagtcgga agaatagtaa
     7441 agggctgccg ccgcgcgcca agctactcgg gcaatttgcg gtggcatttg tcactgctgc
     7501 cattctttgc gtgtacctta agttcaatac ggagctgggc attccgtttg tcaaaaacat
     7561 ccgtcctgac ctggggttgt ggtacatccc gtttgccatg tttgtgattg tcggcgcttc
     7621 gaacgccgtc aacctgaccg atggcctaga tggtttagcc attggtccgg tgatgattac
     7681 cgccggcacc tacgccgtca tcgcctacgt aacgggtcat ttgaaattgg ccgagtatct
     7741 gcagattccg tatgtcgcgg gcgtcggtga actgagcgtg ttttgcgcgg cagtggtagc
     7801 cgccggcttg ggattcctct ggtacaacgc ctatccagcg cagatgttta tgggcgatgt
     7861 cggctctctg tctctgggga cggcgttggg aatagttgcc gtcatgacca agaacgagat
     7921 tcttttgatc atcgtcggcg gcgtttttgt gatggaggct ttgtccgtta tcttccaggt
     7981 tgtttcctac aagctccgac gtaaaagagt tttcttgatg gcgccgatcc accatcacta
     8041 tgagctcaaa ggttggccgg agccgcagat tatcgtgcgt ttttggatta tttcctttat
     8101 ttgcgccgtc gtgggattga gcaccttaaa gcttagatga aaccggcgat gaggcatgga
     8161 gctcagagac aaacaagtgc tggtcgtggg gctagcccgc acgggtattg aatgcgcgcg
     8221 tttccttgtg aatcaagggg ccaaggtgtt ggttagcgat ctgcgatcgg aaacggatct
     8281 cgaacaggca atccataccc tcaagggctt gccgatcgat tatcttttag cgggtgaaga
     8341 gcgccggtgg ttagaaggga tggatatggt cgtgcccagc ccgggtgtgc cgccgacgaa
     8401 tccgttgctc caggaggcaa ccagccgtgg catcgagatc ctcagcgaaa tcgaggtcgc
     8461 ctatcggttt tgccgttttc ctatcgtcgc cgtcacgggc accaatggca agagcacgac
     8521 gacgactctg gtgggagaga tgctcaaggc gaacgggacg aaagtcttca ccggcggcaa
     8581 cattggcgcg ccgttgatcg gatttgtcgg cggtgattgg gaatggggca ttgtcgaaat
     8641 cagcagcttc caattggagt ggattgaaga gttccgtccg cgggtggcgg tgttgctgaa
     8701 tttgtctgaa gaccatctcg atcgttatcc ggactttgcc gcctattgcc gtgccaagga
     8761 gaggatcctc gaaaaccaga cagcgaagga tgtcgcggtt ctcaatcgcg acgatccact
     8821 cgtatggggc ttgcggcagg gaaggcgcgc aagaattatc tccttcggct tctccgaagt
     8881 cgacgaaggc gtttttgcga aatcggagga aattgtttgg cgtgacggtt ccagcgaaga
     8941 acgattcccg ctgggccatg tgaaaattca aggcgtgcac aatgtggaaa atatgatggc
     9001 ggccattgcc acggcgaaag ccatcggcat accggcgcaa atcatccagc agactttgga
     9061 agaatttccg ggtctcgagc atcgtctcga gttcgtcaga gagcaggacg gtgtgcgtta
     9121 ctacaacgac tccaagggga ccaatgtcgg cgcggttgtc aagtcgctgg cgagtttttc
     9181 cacaccggtc attcttcttg ccgggggggt tgataaaggc ggcgactatc gggtgctacg
     9241 agacggcgtc ggaaaaagag tcaagc
//
LOCUS       DASSWZ010000178         7648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_416355, whole genome shotgun sequence.
ACCESSION   DASSWZ010000178 DASSWZ010000000
VERSION     DASSWZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416355"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..3787
                     /gene="smc"
                     /locus_tag="VFN58_04400"
     CDS             233..3787
                     /gene="smc"
                     /locus_tag="VFN58_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET8917265.1"
                     /translation="MRLKSLEMVGFKSFVEKTIIELNPGITAIVGPNGCGKSNIVDAL
                     RWAMGEQSARHLRGHQMEDVVFNGSETLPPTGMAEVSLIFENEDGRGPVEYSSFSEIM
                     VTRRLFRSGESEYAINKIPCRLKDVIELFLGTGVGSKAYSIVEQGRVDELVNSKPEDR
                     RALIEEAAGTSKYKSRKLVAERKLERTQQNLLRITDIVREIERQIRTMELQAKKAERY
                     RALKSQLKEKDLLYAAIQKHVLSEEISGSESQLADVENRFTEHVTALHSKEAESETVR
                     LSLMEAEQKISVQQETVYQVRIQIQAEEQRIDFYRKDLAQLTQAEMETRAALLQLEEK
                     TKALGREIAELEKARESFVQLSLFEESFLQEKEADLNKLQAEVKFLQCDLDREKEALI
                     EAANQIAYLRNDALAKSHRCEEISKELGRSREEHAAASTSVALCEQKLTDTKTTQENC
                     LAQSRDRGVEAAEVNALIQTLLEAKEDQEKRSESLKDQIYENRSRLVSLEDLQKNYEG
                     CQEGVRAIMLKRQHETGSNGIYGLVAEVIEAPEPYEKALTAVLGDRLQYMIVKGQEEG
                     VEAIEYLKREASGRGSFIPLQLSRKQHRPLPLGEAEVIAPLLEVISVKEGYGEVAEYL
                     LSDVVVVRDLRAGLSLWNRNGFYSTLVTPDGEVIDPMGTVSGGSDDALDGNFLTQRRH
                     IRELKTLLAELDAKLQIEDREVEKLKRELERAETKKSSLATEMHRLELDRVRFEHERL
                     IATRDFERFTQTVQALLQEQTDLVSALERGTQEVDQCRIAIETRTQDKIEKENILAQK
                     QAASRELQQSVEAVEAAVTQSRIRNAALGEKKENTHHNLENRLKLQQETFEQINFRQD
                     QIADMAQRRSQIELALAQSEKALQSAKSNLQDLEEDLQTERRNHKEISRNLLDIEESI
                     KELRPLGEACQEEKNRIQVSLAEKKLRCQHLSANVRERYDADLESLRFAGSEPLASTE
                     DLLAEIDDLRARLERMGEVNLAAIGEYEDLNTRFQFMNQQKEDLEKSIADLQRTIIKL
                     NRICRLRFKESFEEINEKFQSIFPRLFRGGKAKLVLTDENDYLETGVDIVVQPPGKKL
                     QFITLLSGGEKALTAVSLLFAIFLTKPSPFCFLDEVDAPLDDANIDRFNDIINEMSQL
                     SQFVLVTHNKKTMQAAEILYGITMAEPGVSKVVSVRMS"
     gene            3853..4779
                     /gene="ftsY"
                     /locus_tag="VFN58_04405"
     CDS             3853..4779
                     /gene="ftsY"
                     /locus_tag="VFN58_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET8917266.1"
                     /translation="MEGSSFFQRLKSGLTKSRENWAQKIGAIFQNRQWDEESLDAMEE
                     NLIAADLGPKATQKLMDLLRQQSPGGAEDLGQEMSSRLQQAMVELLQASTTSPKTLPL
                     SIRPWVIIFLGVNGVGKTTTIAKLAAQYRSAGKKVLLVAADTFRAAAIEQLEAWAQRV
                     GVDVVKHRAGADPSAVVYDGIQAAKSRGIDVLLIDTAGRLHTKVHLIEELKKIRRVIS
                     REQPDAPHETLLVLDATTGQNGLQQARVFKEATDISGIVLTKLDGTAKGGVIVSIQEE
                     LGVPVRYVGVGEDVEDLQPFDASQFVRALFEK"
     gene            4855..5223
                     /locus_tag="VFN58_04410"
     CDS             4855..5223
                     /locus_tag="VFN58_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET8917267.1"
                     /translation="MAGEPNHEGIETEALNLTLTEKAIEQVKALLARDNHKDHGLRVS
                     VADGGCSGFSYKLDFAKEEAPGDLVLELNNLKVYVDPNSVAHLKGTTIDYVTSLYGGG
                     FKFSNPNASGTCGCGTSFSA"
     gene            5384..5941
                     /gene="rsmD"
                     /locus_tag="VFN58_04415"
     CDS             5384..5941
                     /gene="rsmD"
                     /locus_tag="VFN58_04415"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET8917268.1"
                     /translation="MMRVIGGNARGRRLKVPKGQALRPTAAKVKEALFNILPHDLSGA
                     KALDLFAGTGNVTIEALSRGAAAAVLVDSSVESGKAIRENVRRLHLTDRTKVWIMPVA
                     RALRLLARHGETFDLIFLDPPYDRQWIDRSLKIIAQGKLLRGSGVLIAEHSIREKVEP
                     RYDLLALHDQRRYGSTLLSFFKQEG"
     gene            5972..6466
                     /gene="coaD"
                     /locus_tag="VFN58_04420"
     CDS             5972..6466
                     /gene="coaD"
                     /locus_tag="VFN58_04420"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET8917269.1"
                     /translation="MAQNNVAIYPGSFDPITNGHVDLVKRTLRVFERVIIAIATNPGK
                     DSSLFTLEERLQMVREVFRGFKRRVQADSFEGLLVDYAERKQARVIIRGLRAVSDFEY
                     EFQMAMMNHRLKPKLETFFMMTGESEFYISSRLVKEVVGLGGNVSGLVPGNVLTKLVK
                     KFNH"
     gene            6477..>7648
                     /locus_tag="VFN58_04425"
     CDS             6477..>7648
                     /locus_tag="VFN58_04425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET8917270.1"
                     /translation="MTTLAERTKLIKPSVTLAIAAKAGKLRSEGIDVVNFSAGEPDFD
                     TPDHIKAAAVEALRKGMTKYTDVKGIEPLRAAIVHKYEQEHGLPYRKDDVLVSCGAKH
                     SLYNVFQAVINSGDEILIPAPYWVSYSDMAVLAGGVPKIIPTNEATGFRITAEQLAAE
                     LTPKTKVFVLNSPCNPTGASYAADELLAIAGVLGKHSCLILADDIYEKIVYDNFEVKN
                     IVALSPALRERTVIINGVSKTYAMTGWRIGYAIGPGDIISAAGKIQSQSTSNPTSIAQ
                     VAALEAISGSQVEVPRMVREFKQRRDVIVDRLNAIQGMHCLKPQGAFYVFPNISALLG
                     RSANGKKLASPCDFADYLLEAAQVAVVPGEDFGSLENIRFSYATGLEDIEKGCERIA"
BASE COUNT         1976 a         2029 c         2087 g         1556 t
ORIGIN      
        1 ataaggaatg gatgcccttt ctcaatacgg tggacgtctc ctactggaac accgagcttt
       61 tagaaaagta gtttactctg taaatcgtcc cacatattgt ggtacgaatt cagaaattcc
      121 ccctcatatt gtaatttctg cttgacagcc ccgcccgcgt gtgaaatatt gcgggcaaga
      181 gcttgcgtcc gagattttcc ctcaccgaac cgccgcaaaa gaattaattt ttatgcgctt
      241 aaaaagcctt gaaatggttg gatttaagtc ctttgtggag aaaaccatca tcgagcttaa
      301 tccgggaatt acggccattg tcggtcccaa tggctgtgga aaatccaata tcgtcgacgc
      361 cctccgctgg gctatgggtg agcagagcgc ccgccatctc cgcggacacc agatggagga
      421 cgtggtgttc aacggcagtg agaccctacc tcccaccggc atggccgagg tgtcattgat
      481 cttcgaaaac gaggacggac gcggtccggt ggaatacagt agcttcagtg aaatcatggt
      541 gacccgaagg ctgttccgtt cgggggaatc ggaatacgcc atcaataaga tcccctgtcg
      601 cttgaaagac gtcatcgagc tttttttggg gaccggcgtg ggcagtaaag cctattccat
      661 tgtcgagcaa ggacgggtcg atgaactggt caactccaag cccgaagatc ggcgggctct
      721 catcgaagaa gcggccggca ccagtaagta caagagccgc aagcttgtgg ccgagcgaaa
      781 actggagcgg actcagcaga acctcctgcg catcacagat atcgtccgtg agatcgaacg
      841 ccaaatccgt accatggagc tgcaggctaa gaaggccgaa cgttatcgcg cgctcaagtc
      901 gcaattgaag gagaaagatc tgctctatgc ggcgattcaa aagcatgtcc tgagcgagga
      961 gatttccggc tctgagtctc aactggccga tgtggagaat cgcttcaccg aacatgtaac
     1021 cgctctccat tccaaggaag ccgaaagcga aacggtgcgg ctatcgctta tggaggccga
     1081 gcaaaagatc agcgttcaac aggaaaccgt gtaccaagta aggattcaga tccaagccga
     1141 agagcaaagg atcgacttct acaggaagga cctcgcccag ctcacccagg cagagatgga
     1201 gacccgcgcg gcgctcttgc aactcgaaga aaagaccaaa gcccttgggc gggagatcgc
     1261 tgagctcgag aaggctcgag agagctttgt gcaactctcg ctcttcgaag agagtttcct
     1321 acaggaaaaa gaagcagatc tcaataagct tcaggcggag gtcaagtttc tccagtgcga
     1381 tcttgatcga gaaaaagaag cgcttatcga agcggccaat caaatcgcct atttgcgcaa
     1441 cgatgcactg gcaaaatccc atcgttgcga ggaaatcagc aaagaacttg gacgaagccg
     1501 tgaagagcat gcggccgcgt cgacctcggt agcgctgtgt gaacaaaaac tcacggacac
     1561 caagacaacg caggagaatt gccttgccca gtctcgtgac agaggcgtcg aagcggcgga
     1621 ggtcaacgcc ttgattcaaa cgcttctcga ggcgaaagag gaccaggaaa agaggtccga
     1681 gtcgctcaaa gaccaaattt acgaaaaccg ctcgagactg gtgtccctgg aagatttgca
     1741 gaaaaattac gagggctgtc aggaaggcgt ccgggccatc atgctgaaac gacagcatga
     1801 gaccggatcc aatggcatct atggtctggt cgcggaggtc atcgaggctc ccgaacccta
     1861 cgaaaaagcc ctaacggcgg tcctgggcga ccgcttgcag tacatgatcg tcaagggcca
     1921 agaagaaggt gtagaagcca tcgagtatct gaagcgcgag gcttcgggtc gaggcagctt
     1981 cattcctctc cagctgtcca gaaagcaaca tcgcccattg ccgctggggg aggccgaggt
     2041 catcgcccct ctgctcgaag tgatatcggt taaggagggc tatggggagg ttgcagaata
     2101 tctcctatcc gacgttgtcg tcgttcggga ccttagggcc ggattgtcac tttggaaccg
     2161 caatggcttc tacagcaccc tggtgactcc ggacggcgaa gtcatcgatc ccatggggac
     2221 tgtgagcggg ggcagcgacg atgctctcga cggaaatttt ctcacacagc gccggcacat
     2281 tcgagaactg aaaaccctgc ttgcggagct cgacgcaaaa cttcaaatcg aagaccggga
     2341 agttgaaaaa ctaaagcgcg agttggagcg ggcagaaacg aaaaagtctt cgctggcgac
     2401 ggaaatgcac aggctagagc tcgatcgggt aagattcgaa cacgagcgtc tgatcgctac
     2461 cagagacttt gaacggttta cgcaaacggt gcaagctcta ctgcaggagc agaccgatct
     2521 ggtcagtgcg ctggaacggg gcacacaaga agtcgaccag tgtcgaattg caattgaaac
     2581 tcgcacccaa gacaaaatcg aaaaagaaaa tatcctggcg caaaaacaag cagcctctcg
     2641 ggagctgcag caatccgtcg aagccgtgga ggctgccgtc actcagtcgc gtatccgcaa
     2701 tgcggcattg ggagaaaaaa aagagaacac ccatcacaat ctcgagaacc gtctcaagct
     2761 acagcaagaa actttcgagc agatcaactt tcgccaggat cagattgccg acatggccca
     2821 gcgacggagc cagattgaac tcgctctggc gcagtcggaa aaggccctcc aatcggccaa
     2881 aagcaatttg caagacttgg aagaggatct gcaaacggaa cggcgcaatc ataaagagat
     2941 ctccaggaac ctgctggaca ttgaggaatc cattaaagag ctgcggcccc tgggcgaagc
     3001 ctgccaagag gaaaaaaata gaatccaggt gtcgctggca gaaaagaaac tgcgctgcca
     3061 gcatctctcc gccaacgtca gagagcgata tgacgccgac ctggagagtc ttaggttcgc
     3121 aggctcggaa cccctggctt cgacggaaga tctgttggcg gaaatcgacg acctccgggc
     3181 tcggctcgag cgtatgggcg aagtcaatct cgctgccatt ggcgagtacg aagacctcaa
     3241 cacccgcttc caattcatga accagcagaa agaggatctc gagaaatcga tcgcagatct
     3301 tcagcgcacg attatcaaac taaaccgcat ttgccggctc cggttcaaag aaagcttcga
     3361 agagatcaac gaaaaattcc agtcaatatt ccctcggctc ttcaggggcg gcaaggccaa
     3421 gctggttctg acggatgaaa atgactattt ggaaacggga gtcgatatcg tcgttcagcc
     3481 ccccggcaag aaactgcagt tcatcacgct cctctccggc ggcgagaagg cactaacagc
     3541 ggtaagcctc ctgtttgcaa ttttcttgac gaaaccgagt cccttctgtt tcctggacga
     3601 ggtcgacgct ccgctggacg atgctaatat cgatcggttc aacgatatca ttaacgaaat
     3661 gagccagctt tctcagttcg tgctagtcac ccacaacaag aagacgatgc aggctgccga
     3721 aatcctctat ggtatcacca tggcagagcc tggagtctct aaagttgtgt ccgtccgaat
     3781 gagctagctc gttcaggtga ctgaccgtcc cgccccatcg ctcacagcct aaaaatttgc
     3841 gacaatagta aaatggaagg cagttcgttt tttcaacggc tgaagagcgg tcttaccaag
     3901 agccgggaga actgggcgca aaaaattggc gccattttcc aaaaccggca atgggatgag
     3961 gagtcgctgg acgccatgga ggagaacctg atcgcggcgg acttaggtcc caaggccacc
     4021 cagaaattga tggatctcct gcgccagcaa tcgccgggcg gcgctgaaga tctcgggcaa
     4081 gaaatgtctt cacgcttaca gcaggcgatg gtggagttgc ttcaggcatc gacaacgtct
     4141 ccgaaaacgc tgccgctgtc aatccgcccc tgggtgatca ttttcttggg ggtgaacggc
     4201 gtcgggaaaa cgacgacgat cgccaagctc gctgcccaat atcgatcggc cgggaagaaa
     4261 gtcctcctgg tggccgcgga cacctttcgt gcggcggcca tcgagcaact cgaagcgtgg
     4321 gcgcaacggg tcggtgtcga cgtggtcaaa catcgggccg gcgccgatcc ttccgcagtg
     4381 gtttatgatg gcattcaagc cgccaaaagt cgcgggatcg atgtgctgct catcgacacg
     4441 gcgggacgac ttcatacgaa ggtccattta atcgaggaac tgaaaaagat ccgccgggtt
     4501 atttcccggg aacaaccgga tgcgccccac gaaactcttc tggtgctgga tgccacgacg
     4561 ggacaaaacg ggctccagca agcccgcgtt ttcaaagaag caacggacat cagcggtatc
     4621 gtactgacca aactcgatgg cacggcgaag ggcggcgtga tcgtgagcat tcaggaggaa
     4681 ctgggggttc ccgtcaggta cgtcggcgtc ggcgaagatg tagaagacct ccaaccgttc
     4741 gacgcctctc aatttgtccg ggccctcttc gagaagtgac cctcacgtcg cttgaatcct
     4801 cccttgactt agctggaagc gagcattacc cttctatctt agcccgagaa aagcatggcc
     4861 ggcgaaccaa accacgaagg catcgaaacg gaagcactca atttgacttt gaccgagaag
     4921 gccattgaac aagtcaaagc tctcctggct cgggacaacc ataaagacca tgggttacgg
     4981 gtgtcggtgg cagacggagg ttgttcgggt ttctcgtata agttggattt tgccaaggaa
     5041 gaagcgcccg gcgacctggt attggaacta aataacctca aggtctacgt agatcccaac
     5101 agtgtagcgc atctcaaggg tacgaccatc gactacgtaa ccagccttta cggcgggggc
     5161 ttcaaattca gtaatcccaa cgcgagcgga acctgcggtt gtggcacttc attttccgcg
     5221 taatcctaaa accctcagcg gcccgctcac tttcactgac tagctttttt cccggccaag
     5281 tcctttttgc ggcggccttt gacactcttc tcatatcttg ctaacgaaaa tactatttct
     5341 tgccggtaga attggattga acttggagag caacaattga gggatgatgc gagtcattgg
     5401 aggaaatgca cgcggccggc gtctcaaggt gcccaagggg caggctctgc gtcctaccgc
     5461 tgcgaaagtc aaagaggcgc tcttcaatat tctcccccac gatctttccg gcgccaaagc
     5521 cttggatctt tttgccggaa ctggaaatgt gaccattgag gctttgagtc gtggcgcagc
     5581 ggcagccgtc ttggtggact catcggtgga atcgggcaag gcgatccgag aaaacgttcg
     5641 ccgacttcat cttaccgacc ggaccaaggt atggatcatg ccggtcgctc gagcgctacg
     5701 gctcctcgcg cggcacggcg agacatttga tctgatcttt ctcgatccgc cgtatgacag
     5761 acaatggatc gacaggagcc tgaagatcat cgcccaagga aaattattac gcggatcagg
     5821 agtactgatc gccgagcaca gcattcggga aaaggtcgaa ccacgttatg atttgctcgc
     5881 gctccatgac cagcggcgtt acggcagtac gctgctttct ttttttaagc aggaaggcta
     5941 aagcaaatca caaggacaag gatgcccaac tatggcacag aacaacgtgg cgatttatcc
     6001 aggctcattc gatcccataa ccaacggaca cgtagatctc gtaaagcgca cgcttcgcgt
     6061 cttcgagagg gtcattatcg ctatcgcgac caatccgggc aaagactctt ctcttttcac
     6121 tctggaagag cgtcttcaga tggtgcggga agttttcaga ggattcaaac gcagggtcca
     6181 agcggattcg ttcgaaggct tgctggttga ctacgcagag cgcaagcaag ccagagtcat
     6241 tatccgcggc ctgcgggccg tgtcggattt cgagtatgaa tttcagatgg ccatgatgaa
     6301 tcaccgcctc aaaccaaaat tggaaacctt tttcatgatg actggagagt ccgagttcta
     6361 catcagctcg cgcctggtga aggaagtcgt gggtttgggt ggaaacgtct ccgggctcgt
     6421 ccccggcaat gtgctcacga agctggtaaa aaagttcaac cactagggat gctaagatga
     6481 caaccctcgc agaaagaacc aaactcatca agccctcggt gacccttgcc attgcggcca
     6541 aagccggcaa gctccgatcg gaaggaatcg acgtggtcaa tttctctgcc ggtgagcctg
     6601 atttcgacac tccggatcac atcaaagcag cggctgtgga ggccctgcgc aaaggcatga
     6661 caaagtacac ggacgtgaag gggatcgagc cattgcgcgc agccatcgtt cacaaatatg
     6721 agcaagagca tggactgccg tatcggaaag acgatgtttt ggtcagctgc ggagcaaaac
     6781 attccctcta taacgtcttt caagcagtca tcaattccgg agacgagatc ctcattccgg
     6841 ccccctactg ggtgagttat tcggacatgg cggtgttagc cggaggcgtt cccaaaatca
     6901 ttccgaccaa cgaagccacc ggttttcgta tcacagccga acagcttgcg gctgagctga
     6961 cgcccaaaac caaagtcttc gttctcaaca gtccctgtaa ccccaccggc gcaagctatg
     7021 ctgctgatga gcttttggct atcgcgggtg tgctgggaaa acatagctgc ttgatcctcg
     7081 cggacgacat ctatgagaag attgtctatg acaacttcga agtcaaaaat atcgtcgcct
     7141 tgagcccggc attgcgcgag cgcaccgtca tcatcaacgg cgtttcaaaa acctacgcga
     7201 tgacggggtg gcgcatcggc tacgccatcg gtccgggcga tatcatttct gccgccggca
     7261 agattcagag tcaaagcacc tccaacccaa cgtccattgc tcaagtagca gcgctcgaag
     7321 ctatttctgg ctcacaagtt gaagtcccta ggatggttcg ggagttcaaa caaagacgcg
     7381 atgtcatcgt cgatagactc aacgcaatac aaggcatgca ttgtctcaaa ccgcaggggg
     7441 cattctacgt ttttccaaac atcagcgcgt tgttgggtag atcggctaac gggaagaaac
     7501 ttgcttcccc ttgcgacttt gccgattacc tcctcgaagc agcccaagtg gctgtcgtcc
     7561 cgggcgagga ttttgggtcc ctggaaaata tccgattctc ctacgccaca ggcctcgaag
     7621 acatagaaaa gggctgcgag cggatcgc
//
LOCUS       DASSWZ010000179         6780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1039027, whole genome shotgun sequence.
ACCESSION   DASSWZ010000179 DASSWZ010000000
VERSION     DASSWZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039027"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VFN58_04430"
     CDS             <1..688
                     /locus_tag="VFN58_04430"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917271.1"
                     /translation="YAAMTLVSRPEIRTAKDLMGGVIGLQRPGDAYEKNARSALQHLK
                     LNPDKDVKFLYLGTNEAMWIALDARKVAATMVSPPATLFARKGGMNFLVNLSDLKVEY
                     QGSTFATRRSLMKNYPNLTLRTVRAMVRGIHFFKTRREDTYKILAKFLGTSDPEALEE
                     SWHYGADMPPKPFAVESAVQAVISHLAEGDPKFAKTKPADFIESGPLAELERNGYIDR
                     LYGGRDTKAR"
     gene            733..2772
                     /gene="uvrB"
                     /locus_tag="VFN58_04435"
     CDS             733..2772
                     /gene="uvrB"
                     /locus_tag="VFN58_04435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET8917272.1"
                     /translation="MIRNREERQFRLISEFKPQGDQPLAIEKLVQGIQNGKKNQVLLG
                     VTGSGKTFTMANVIARINKPTLVIAPNKTLAAQLYNELRELFPDNAVRYFVSYYDYYQ
                     PEAYVPSTDTYIEKDASINDEIDKLRHSATKALLERSDTLIVASVSCIYGLGSPEAYF
                     EMMVYLEEGGRIQRDQMLRKLVDIQYQRSDFDFHRGTFRVRGDIVEIFPAYEDSSALR
                     IEFFDEQIETIHEIDPIRGKIQRRVDKACIYPASHYVTGEDRMKQATQDIRLELKERL
                     EVLRAEHKLLEAQRLEQRTLYDLELLEEMGFCPGIENYSRHLTGRKPGEPPPTLLSYF
                     PNQFLLFIDESHVTVPQIGGMYRGDRSRKETLVEYGFRLPSALDNRPLNFAEFEACLD
                     QIIYVSATPAHYELERSRGGVVEQLIRPTGLTDPELVVRPARNQVDDLLEEIRKRAER
                     RERVLVTTLTKRMAEDLTDYYQDLNVKVRYLHSDIETLERVEIIRQLRMGVFDVLVGI
                     NLLREGLDLPEVSLVAILDADKEGFLRSDRSLIQTIGRAARNVHGTVILYADSITGSM
                     RLAIDETNRRRGLQAAFNTEHGITPQTVIKSLGSPLVKIYEADYVDVPLAAEKEANYG
                     TKEFPRAIQRLKKEMKKAAESLEFERAAELRDRIRAMEEQEIGLRNPLAARSPGS"
     gene            complement(2824..3237)
                     /locus_tag="VFN58_04440"
     CDS             complement(2824..3237)
                     /locus_tag="VFN58_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917273.1"
                     /translation="MFDIENLMHIGVFNQLVPIWQVFFFIASLLPFLLWNRVKICLLI
                     TYMFTFYLGFMVQWGDYIATTGAMQPFVLYSLSGLAIAVFFVATIFSEQPEQTKVRWV
                     RYSQQADNAERPTRMRHSDPVEAAINVTNVSDTEH"
     gene            3525..5372
                     /gene="uvrC"
                     /locus_tag="VFN58_04445"
     CDS             3525..5372
                     /gene="uvrC"
                     /locus_tag="VFN58_04445"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET8917274.1"
                     /translation="MAITPENIANLSSRPGVYLMRDDRGKVIYVGKAKDLRARVRAYF
                     HSPDGRCQVEFLVRRVADIETLVTSNDKEALILENNLIKQYKPRYNIRLKDDKSYLSI
                     KVTTQHAWPRIFTTRKIVKDGNRYFGPFSSAVAARETLDIVEKHFLLRNCTEHNFKNR
                     SRPCLQYQIKRCLAPCVLPVSQPDYRERVRQAILFIEGKQDELLSELRQKMAEKSEAM
                     EFEAAAKIRDQIQAVEKTLEKQRMVSHWGSDQDVFGLYREGGFIEVQVLFVRQGKLTG
                     SQSYSLEDLEFPDEEVMGALLQQFYQGHRFIPDEILLPVELDDREVREDYLRENKGKR
                     VSILSPQRGAKNELVEMARENARQSFSERHDQAKAREKMLKELQSKLRLRNYPQRIEC
                     FDISMLHGAHAVGSMVTFVNGEPDKSRYRHYRIRSIDASSGGDDFGMMLEVLKRRFAR
                     GREEEDLPDLVVVDGGKGQLAMALAAMADVGVKGVEAVGLAKMRVQSAPRSAEIERSE
                     ERVFLPGQSNPVILKRNSNALFLLQRVRDEAHRFAITHHKKLRTRQTLFSTLDRIPGV
                     GGARKRALLRAFGSIKRIEKATLEELLKVPSMNEKTAQEILQSLRSGLD"
     gene            5385..>6780
                     /locus_tag="VFN58_04450"
     CDS             5385..>6780
                     /locus_tag="VFN58_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET8917275.1"
                     /translation="MSCETATGTAAVAKPGHPLVWLVVPTLALLVGQAVATFPLVIPL
                     QIVFLPLIPLLLVIKATTRRWGLLIFAASLAFSVGYIRHWQLLHPHFPPNHVQLLSND
                     SARLYLEGTLAREPEKLPNRSRWLLRCERIWYPTGAEEISGNLLVSIRQVRREWHYGD
                     RVRFWIRPQAPRNAGNPGGFDYATYLARQEIYAIGYLESDQEVEQVARRAGRVRGAIE
                     SLRREIRRYIDQHVSSDNGALMKALVVGDMGAVTREMRNEFTAAGVNHVLSISGLHVS
                     MLGLVVFWLVRFGCSYSTYLTLRCNLVKVGTFFSFMAVVFYTAIAGAMVPTVRSAIMI
                     GVYELAVLLDREEEVFASLTFAALIIALIWPAVIADISFQLSFLAVLFIVWGMRRMMD
                     GSPRRPRDELPQERSWWKQKLVQGRLYLAVPLFATLGTGPLIAHYFGHLSLAGFISNP
                     VIVPLVGFIVVPLGL"
BASE COUNT         1595 a         1718 c         1927 g         1540 t
ORIGIN      
        1 ttacgctgcc atgacgctgg tgagccgccc ggaaatccgc actgccaagg atttgatggg
       61 cggagtcatc ggtctgcagc gtccggggga tgcctacgag aaaaatgcgc gctcggcgct
      121 ccaacatttg aaattgaacc cagataaaga cgtcaagttt ctctatcttg gaaccaacga
      181 agcgatgtgg atcgcgctgg atgcgcgcaa agttgcggca acgatggtgt cgccaccggc
      241 gacgttattt gcgcgaaagg gaggcatgaa cttcctcgtg aatctctcgg atctgaaggt
      301 ggaatatcaa ggctctacat tcgcgactcg ccgctccctg atgaaaaact acccgaatct
      361 tacattgcgc acagtgcgtg ccatggttcg gggcattcat ttcttcaaaa cccggcgtga
      421 ggacacgtac aaaatccttg caaagtttct cggcacgagt gacccggagg cgttggagga
      481 atcgtggcat tacggtgccg acatgccgcc caaacctttc gctgttgaaa gcgcggttca
      541 agccgtgatc agtcatctgg ctgaagggga tccgaaattt gccaaaacca agcctgccga
      601 cttcatcgag tctggtccgc ttgccgaact cgagcgcaac ggctacatcg atcgtctcta
      661 cggcgggcgg gatacgaagg cgaggtaacc acccgcttgt ccgatccccc gtgggctgat
      721 attttttctc taatgattcg caaccgagaa gaacgtcagt ttcgattgat ttccgagttt
      781 aaaccccaag gcgaccagcc gctggccatc gagaaacttg tacagggtat ccagaacggt
      841 aaaaagaacc aggtcttgct gggggtgacc ggttccggca aaacgttcac gatggccaac
      901 gtgatcgccc ggatcaacaa gccgaccttg gtcatcgcgc ccaataaaac tcttgcggca
      961 cagctttaca acgagcttcg cgagctattt cccgataacg ccgtgcgcta ctttgtcagc
     1021 tattatgact actatcagcc ggaggcttac gtgccgtcga ccgacacgta cattgagaaa
     1081 gacgcctcta tcaatgacga gatcgacaaa ctacgccact ccgcaaccaa agccctgttg
     1141 gagcgaagcg atacgctgat tgtcgcgagc gtctcctgta tctacggcct cggctcgccg
     1201 gaagcttact ttgagatgat ggtgtatctc gaagaaggcg gccggatcca acgtgaccag
     1261 atgctgagaa agctggtcga tattcaatat cagcgcagcg actttgattt ccatcgcggt
     1321 acctttcgag tccggggaga tatcgttgag atctttccag cttatgagga ttcgtcggct
     1381 ctccggattg aatttttcga tgaacagatt gaaacgattc atgaaatcga tcccatccgg
     1441 ggaaaaattc aacggcgggt cgataaagcc tgtatctatc ccgccagcca ctatgtgacc
     1501 ggcgaagatc gaatgaagca ggcgacgcag gatatccgct tggagctcaa agaacgcctg
     1561 gaagtgcttc gcgcagagca taagctcttg gaggcgcaac ggctggaaca acgaacgctc
     1621 tatgatttgg aattgttgga agagatgggg ttttgcccag gcatcgaaaa ttattcgcgc
     1681 cacctgacgg ggcgaaagcc gggggagcct ccgccgacat tgctcagcta ctttccgaac
     1741 caattcctgc tcttcataga cgagagtcat gtcactgtgc cccaaatcgg cggtatgtat
     1801 cgaggcgacc gatccaggaa ggaaactcta gtcgagtacg gtttccgttt gccttcggcc
     1861 ttggacaatc gccctttgaa ttttgccgag ttcgaggcgt gcctggacca gatcatctat
     1921 gtttctgcca ctccggcgca ctatgagttg gaaaggagcc gggggggcgt tgtcgagcag
     1981 ctcattcgcc ctacgggctt gacggacccc gagctggttg tgcgtccggc gcgcaatcag
     2041 gtggacgatc tacttgaaga aatacgcaag agagccgagc gaagagaacg agttctggta
     2101 acgactttaa ccaaaagaat ggcagaagat ctgacggact actaccaaga tctaaacgtg
     2161 aaggttcgct atttacactc ggatattgag accctcgagc gggtcgagat catccgacaa
     2221 ttgcgcatgg gcgtttttga tgttttggtc ggcattaatc tcctccgcga aggactcgac
     2281 ctcccggaag tatctttggt cgctatactc gatgccgaca aagagggctt cctgcgctcc
     2341 gatcgttctc tcatccagac gatcggacgg gcggcgcgca atgttcatgg gacggtcatt
     2401 ctctatgccg actccatcac cggttctatg aggctggcca ttgacgaaac caatcgccgc
     2461 cgtggtttgc aggcggcttt caatacggag catgggatca ccccgcaaac agtgatcaaa
     2521 tctctcggtt caccgcttgt gaaaatttac gaagccgact atgtcgatgt tcctttggcg
     2581 gcagagaagg aagccaacta tgggacaaaa gaattcccta gggcaattca aaggttaaaa
     2641 aaagagatga aaaaagctgc cgaaagtctt gagttcgaga gggctgccga attgcgcgac
     2701 cgcattcggg cgatggaaga acaagaaatc gggctgcgta atccgttggc ggcacgcagc
     2761 ccgggttcct agcctcgaaa caacttacga ggtgaaagac aacctgggtg gtagctgaga
     2821 ttctcagtgc tcggtgtcag aaacattggt gacgttgatg gcggcttcca cggggtctga
     2881 atggcgcatc cgagtcggcc tttctgcgtt atccgcttgt tgagagtaac gaacccagcg
     2941 aactttggtt tgttccggtt gctcgctgaa gatcgtcgca acaaagaaaa ccgcgatcgc
     3001 caaaccactg agggaataga ggacaaaggg ctgcatcgca ccggtggtgg cgatgtagtc
     3061 tccccattgg accatgaagc cgaggtaaaa ggtaaacata taggtgatga ggaggcagat
     3121 tttgacgcgg ttccatagta gaaacggcaa gagactcgcg ataaagaaaa acacctgcca
     3181 gatgggtacc agttggttaa agaccccaat atgcatcagg ttttcaatgt cgaacatgtt
     3241 atactccgtt ttatttgcgt caatttttcg tcgcgtcgtc taaacagtaa gagtccaaaa
     3301 gccatgccaa tttacctccg gtggattggc cgcattatga ttgaaaaaac ggcgaaggac
     3361 ggtaggaaat tgcaaaaaat gtccgccaga gtgcaaatgt ggacacgact ggagaactta
     3421 ggcagtgtca atttggcagg taggatccgt ccagtcggcg actccgttaa tcctcaattt
     3481 aaaggagccc gcttttttcc aacgatcgtg tctggcaaat tcccatggca atcactccgg
     3541 aaaacatagc aaatctatcc tcacgtcccg gtgtttacct aatgcgggac gacagaggaa
     3601 aagtcatcta cgtcggcaag gccaaagatc ttcgcgctcg tgtgcgagcc tattttcata
     3661 gtccggatgg acgctgccag gtcgagtttc tcgtgcgacg cgtcgccgat atcgagactt
     3721 tggtcacgag taatgacaaa gaagcgctga ttctcgaaaa caatctcatc aagcaataca
     3781 agccgcgcta caatatccga cttaaagacg acaagagcta tctgagcatt aaagttacga
     3841 cacagcacgc ctggccgaga atcttcacga cgcgtaagat cgtcaaggac ggcaaccgtt
     3901 attttgggcc attttcgtcg gcagtggcgg ctcgagaaac gctggacatc gttgagaagc
     3961 attttctttt gcgcaattgc accgaacaca actttaaaaa ccgcagccgg ccctgtctcc
     4021 aatatcaaat caaacgttgc ctggcaccct gcgtcctgcc cgtaagccag ccggattacc
     4081 gggagcgagt ccggcaagcc attttgttta tcgagggcaa gcaagacgaa ctgctatcgg
     4141 aactgcggca aaagatggca gagaaatctg aggcgatgga gtttgaagcg gcggccaaga
     4201 ttcgcgacca gattcaggcc gtcgaaaaaa ccctcgaaaa gcagcgtatg gtttcccact
     4261 ggggaagcga ccaggatgtt tttggacttt atcgggaggg agggttcatt gaggttcaag
     4321 tccttttcgt gcgccagggc aaattgacgg gtagccagtc ttattcgctg gaggacttgg
     4381 agtttcctga tgaggaggtc atgggcgcgt tgctacagca attttaccag ggccatcgct
     4441 ttatcccgga tgaaattttg ctaccggtgg agttggatga ccgggaagtt cgggaagact
     4501 acctgaggga aaacaaggga aagcgagtga gcattcttag cccgcagaga ggcgcgaaaa
     4561 atgaactggt ggagatggcc cgggaaaacg ctcgacagag cttttcggag cgccacgatc
     4621 aagcgaaagc gcgggagaag atgctaaagg agcttcagtc gaagctgcgc ttgagaaatt
     4681 atccgcagcg aatcgagtgt tttgatattt cgatgttgca tggcgcccac gccgtcggtt
     4741 ccatggttac ctttgtcaat ggtgagccag acaagtctcg gtatcgccat taccggattc
     4801 gaagcattga tgcctcttcc ggtggggatg acttcggcat gatgttagaa gtgttgaagc
     4861 gccgtttcgc ccgcggtcgg gaggaagaag atctgccaga tctcgtggtg gttgacggcg
     4921 gcaaggggca gctcgccatg gcactagcgg cgatggctga cgttggagtg aaaggtgtgg
     4981 aagcggtggg cctggcgaaa atgcgcgtgc aatcggcgcc gcgcagcgcc gaaatcgagc
     5041 gctccgaaga gcgcgttttc ctgccagggc agagcaatcc agtgatcctg aagcgcaact
     5101 cgaacgcgct tttcttgctg cagcgagtgc gggacgaggc gcatcgtttt gccatcacgc
     5161 atcacaaaaa actacggacc cggcaaacac tattttccac tttggaccgt attccgggag
     5221 tcggaggagc gcgcaagcga gccctgctgc gcgcttttgg tagcatcaaa agaatcgaaa
     5281 aagcaacatt ggaagaactc ctcaaggttc cttctatgaa cgagaagact gctcaagaga
     5341 ttcttcaatc ccttcggtct gggctggatt gacggaaagc gcagatgtcc tgcgagacgg
     5401 caacagggac ggctgcagtc gcgaagcccg gccatccgtt ggtctggctg gtggttccga
     5461 cgttggcgct cctggtaggc caggcagtgg ccacgttccc gctggttatc ccgcttcaga
     5521 ttgtttttct cccgctcatt ccgttgttgc tagttatcaa agcaacaacg cgacgttggg
     5581 gactgctaat cttcgccgcg agtctggcct tttcggttgg ctacattcgg cattggcagc
     5641 tgctccaccc gcacttcccg cctaatcacg tacagctact gagtaacgat agtgcacgcc
     5701 tctatctgga agggacactc gcgcgagaac cggagaaatt gccgaaccgc agccgttggc
     5761 tgctgcgatg cgaaagaatt tggtatccca caggagctga agaaatctcg ggaaaccttt
     5821 tggtgagcat ccggcaggta cggcgggaat ggcactacgg cgaccgcgta cgcttttgga
     5881 ttcgaccgca ggcgccgcgt aacgccggca acccgggtgg tttcgattat gcaacgtacc
     5941 tagcgcgaca ggaaatttat gccatcggat acctggaaag cgaccaggag gtcgagcagg
     6001 tggcccgcag ggctggtcgt gtgcgtggcg ctatcgaatc gctgcgcaga gaaattcgcc
     6061 gctatatcga tcagcacgtt tcatcggata acggcgcgct tatgaaggcg ctcgtggtcg
     6121 gcgatatggg cgctgtcacc agggaaatgc gcaacgagtt taccgctgcc ggtgtgaacc
     6181 acgtgctttc gatttccgga ctccacgtca gcatgttggg gctagtggtc ttctggctgg
     6241 tccgcttcgg ttgctcctac agcacctacc tcacgctacg ctgcaatctg gtaaaggtcg
     6301 ggacattttt ttctttcatg gcggtggtct tttacaccgc catcgccggg gcgatggttc
     6361 ctacggtccg ttcggcgatc atgatcggtg tctatgagtt ggcggtttta ttggaccgtg
     6421 aagaggaagt gtttgccagt cttacttttg ccgcgctgat catcgcgctg atctggcccg
     6481 ctgtgattgc cgacatttcc tttcagcttt cctttcttgc ggtgctgttt attgtttggg
     6541 gcatgcgcag aatgatggat gggtcccctc ggcgaccacg cgacgagctg ccgcaggagc
     6601 ggagttggtg gaaacaaaaa ctggtgcagg ggagactgta tttagccgtg ccgctttttg
     6661 caaccttggg caccgggccg ctgatcgcgc actatttcgg tcatttgtca ttggccggtt
     6721 ttatttccaa cccggtgatt gtgcctttag tcggttttat cgttgtgccg ttgggactgg
//
LOCUS       DASSWZ010000180         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1095438, whole genome shotgun sequence.
ACCESSION   DASSWZ010000180 DASSWZ010000000
VERSION     DASSWZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095438"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFN58_04455"
     CDS             <1..433
                     /locus_tag="VFN58_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8917276.1"
                     /translation="EFDKAMAVNVRGSWLCAKAAFPYMKKQGKGKIINISSGTALNGG
                     QNYIHYVTSKGALIAMTRAMAREVGDYNICVNTVCPGFVVTEGRQVDPAFSANRVANR
                     ALKRVQVENDLVGTVLFLASPESDFMTGQLLNVDGGAHFVG"
     gene            688..1431
                     /locus_tag="VFN58_04460"
     CDS             688..1431
                     /locus_tag="VFN58_04460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET8917277.1"
                     /translation="MRFENKVVIITGGGGKIAKAYAMAFAREGAKVSLPDVASADPIV
                     RAIKDMGGTAISMSCDVSNEKSVKAMVDETVRQFGTVDVLINNAAYFMTVWKGPFWEM
                     TVEEFDQAMAVNVRGSWLCAKAVVPHMQKQQQGKIINISSNVALTGNPNYIHYVTSKG
                     ALIAMTRAMARELGDYNICVNTVSPGFVVTEGRQVDPAYEKVRAQQRSIKRSQVETDL
                     VGTVLFLSSPQSDFMTGQLLNVDGGFHFV"
     gene            complement(1546..>2721)
                     /locus_tag="VFN58_04465"
     CDS             complement(1546..>2721)
                     /locus_tag="VFN58_04465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET8917278.1"
                     /translation="ALPGNIIQGIRKAGSRNPVFMLDEVDKLSASFHGDPSAALLEVL
                     DPAQNATFQDHYIALPFDLSQVLFIATANVLDTVPGPLRDRMEILELPGYTEEDKLAI
                     AKGYLIPRQISENGLNPGEITFTDDAIREIIRSYTREAGVRQLERQLGAVCRSVATRV
                     ADGLKEIITVNKESLATYLGPQKFFNEIALRTSLPGVATGLAWTPFGGDILFIEATKM
                     PGDGKLLLTGQLGDVMKESAQAALSLVKSRAETLGIDPDIFKKNDLHIHIPAGAIPKD
                     GPSAGVTLFVALVSLLTGRRISKDVAMTGEISLRGLVLPVGGIKEKMLAAKRAGISCV
                     LLPELNRKDLEEIPPAGRQGIRFEFLQTADEALRLALEPEAPGGFSPGDRLAARPLS"
BASE COUNT          604 a          746 c          776 g          595 t
ORIGIN      
        1 cgagttcgac aaagctatgg cggtgaatgt ccgcggctca tggctgtgcg ccaaagcagc
       61 ttttccgtat atgaaaaagc aaggcaaggg gaaaatcatc aacatctcgt cgggtaccgc
      121 gttaaacggt ggccagaact acatccacta cgtgacttcc aaaggcgcct tgatcgcaat
      181 gacgcgggcg atggcccgcg aagtcggcga ttacaacatc tgtgtcaata cggtctgccc
      241 cggctttgtc gtaacggaag ggcggcaggt tgatccggca tttagcgcca accgggtcgc
      301 taaccgcgcg ttgaaacggg tccaggtgga gaacgattta gtcggcacgg ttctattcct
      361 ggcttcgccg gaaagtgatt tcatgaccgg gcaattgctc aacgtagacg gcggggccca
      421 ctttgtcgga taaggacttt tactgagtga tttaactcaa agccggcatt atttcgaagc
      481 tgctcgggcg atgagtccta cagccgtggg caatgggaca gtgccttgag ggccaccccg
      541 caatccccta agggtgatgt ttcgtacggt cagtgccttg agcaacctgc acgctcaggt
      601 gcccacggct tccggtctca ccgccctcgc tcattgctcg cgctgcgcaa gttgtgtcgg
      661 attagggcta actggaagga tttaaaaatg cgtttcgaaa acaaggtggt catcatcacc
      721 ggcgggggcg gaaagatcgc caaagcttac gcgatggctt tcgccagaga aggagcaaag
      781 gtttcgctgc cggacgtggc cagtgcggac cctatcgtta gggcgatcaa ggacatgggc
      841 ggaacggcta tcagtatgag ctgcgatgtg tcgaatgaga aaagtgtaaa agcgatggtc
      901 gatgagacgg tcaggcaatt cggcactgtc gatgttctca tcaacaacgc cgcttacttc
      961 atgaccgttt ggaaagggcc gttctgggaa atgacggtgg aggagttcga tcaagcaatg
     1021 gcggtcaacg tccgcggttc gtggctgtgc gccaaagccg tcgtcccaca tatgcagaag
     1081 cagcagcaag gaaagatcat caacatttcg tcgaacgtcg ccctgaccgg caatccaaac
     1141 tacattcact atgtgacttc taaaggggcg ctgatcgcga tgactcgcgc tatggcccga
     1201 gagttgggcg actacaatat ctgtgtcaac acggtgagcc cgggtttcgt tgtcacggaa
     1261 gggcgacaag ttgaccccgc gtatgagaag gtacgcgccc agcagcgcag catcaaacga
     1321 tctcaggtcg aaaccgattt ggtcggcacc gtgttgtttc tatcttcccc acaaagcgat
     1381 tttatgaccg gccaattgct gaatgtggac ggcgggttcc atttcgtctg attcagactg
     1441 gaaaccgcaa agatcccaaa aagctctttc gtgggacggt ggccacgcct gctctcctca
     1501 ccactgccgc taacattcgc aagcggccct tcgggcttac cgctctcagg aaagcggacg
     1561 agccgcgagt ctgtcaccgg gagaaaaacc gcccggggct tcgggctcca gtgcaagccg
     1621 caaggcctcg tccgccgttt gtaaaaactc aaagcggata ccctggcggc ctgcgggggg
     1681 aatctcttcc aagtcttttc ggttcaactc aggcagcaac acacaggaaa tccccgctct
     1741 ttttgctgcc agcatcttct ctttgatgcc tcctaccggg agcaccaagc cgcgcagact
     1801 gatctcgccc gtcatggcga catctttgct gattcgccgt ccagtcagaa gtgagacgag
     1861 agcgacaaac agtgtgactc cagcactggg accgtccttc gggattgcgc ccgcgggtat
     1921 atgaatgtgc aagtcgttct tcttgaagat atcgggatcg attcccagtg tctccgcgcg
     1981 ggatttgacc agactcaaag cggcctgcgc gctttctttc atcacatcgc cgagctggcc
     2041 ggttagaagc aacttgccgt ctccaggcat tttcgtcgct tcgataaaca agatgtcccc
     2101 gccaaacggc gtccaggcca agccggtcgc cactccaggc aagctggtgc gcagcgcgat
     2161 ctcgttgaag aatttttgcg ggcccaagta ggtggcgaga gattctttat tgacagttat
     2221 gatctcttta agcccatccg ccacgcgcgt tgccacactg cggcagaccg ctcccaactg
     2281 gcgctcgagc tggcgcaccc cggcttcgcg tgtgtaggac cggatgattt cccggatcgc
     2341 gtcgtcagtg aaagtaatct ctccgggatt aagaccgttt tcagaaatct ggcgtggaat
     2401 gagatatccc ttggcgatag ccagcttgtc ttcctcggtg tatcccggca gttccaagat
     2461 ctccattcgg tcacgcagag ggcccggaac cgtgtcgagc acgttagccg tcgcgatgaa
     2521 caatacctga cttagatcga agggcagcgc gatgtaatgg tcttgaaaag ttgcattctg
     2581 agcgggatcg aggacctcga gcaacgcggc ggatgggtcg ccatgaaaac tagcgctgag
     2641 cttgtcgact tcatccaaca tgaagacggg gtttctactg ccggcctttc tgattccttg
     2701 aataatattg cccgggagcg c
//
LOCUS       DASSWZ010000181         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1180622, whole genome shotgun sequence.
ACCESSION   DASSWZ010000181 DASSWZ010000000
VERSION     DASSWZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180622"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1347)
                     /locus_tag="VFN58_04470"
     CDS             complement(256..1347)
                     /locus_tag="VFN58_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917279.1"
                     /translation="MSIKIHKIIDGDGHVVEDHAAIGTRMPDEYRDRFGVGRGPYPPN
                     DHLHAANAHFLPPGSFVKVGREGWIDFMEDVGLASAVLYPTSGLSFGRIVSRDWAVEL
                     ARAYNDWMYDEYLSKSSRFQALGLIPLQEPAEAVKELRRIVKELGFCGAMLPSTGAVG
                     QQNQLGDDRYWPIYEEANRLECCIGIHGGVHDHMGLDDMSPYAPVNALGHPFGQMVNF
                     AGILFSGVFDTHPKVRFGFMEAGSGWFINCVERFERSWSSHVQHDPRGRFLKLRDKES
                     ITDYIRRHVDEDRIFVGVEGDELTLPFAVSVVGNKPFIFSSDFPHEVNNETCKAELEE
                     LDENGRLTKADKDAVRYRNAERFYGLRGD"
     gene            complement(1363..2469)
                     /locus_tag="VFN58_04475"
     CDS             complement(1363..2469)
                     /locus_tag="VFN58_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917280.1"
                     /translation="MSTSARHQIIDGDGHVVEDISTIWKYMPQEYVGRSFSDARGRSP
                     FPPIDHLHSANRHFTPKGAFANVGREGWELFLEEVGIGTTVLYPSAGLAFGKIVSRDW
                     AIELARAYNNWIYDTYLSKSNRFKAMGLIPLQEPAEAVIELRRIVRDLGFSGAVLPST
                     GANMPHLGSEKYWSIYGEAERLGCAIAIHGGAHEGLLMDDLSPYAPVNALGHPMSQMI
                     CFAGIIFNGILDKFPGVRIGFMEAGSAWLLTCMERFTGSWESHVQHDPRGRFLQLRKG
                     EKIIDYILRHIDEGRIFVGCEGEELTLAEAVRISGNKPFVFSTDYPHEVDAETCKHEL
                     EELRENTQLTADDKEAILFRNSQRFYQLTSRKPE"
     gene            2599..>3459
                     /locus_tag="VFN58_04480"
     CDS             2599..>3459
                     /locus_tag="VFN58_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917281.1"
                     /translation="MLGTEVERDPWREARMIRHVQILFIVWGVSLALAAPSRCNAEIF
                     RIGFPSLATGFAPSWVTVEKGIWKKHGLDVELIYLRGGSRTVSALIGGSVDFILGSDL
                     GVTTAILQGAGLTRVGVTTNTLGYSMVTQPNIKTVRDLKGKIIGITPGRDAAYARVVK
                     LLRDNGMDASKDVTFLSVGDGGPASRVAALSSGVIHATMFTPPSDLISEKAGMRILTK
                     IDVANVGGGLNTTPGFIQKSRSRALRFLRGYMEGIQYMKSHKDESLKIFSKYVRNPDL
                     AIMAYLYEEIV"
BASE COUNT          754 a          960 c          950 g          795 t
ORIGIN      
        1 atttaagtcg ctatttcctt tcaactttga tgacgggaaa ctctgctgcg tggcccagcc
       61 gcaccacccc gttgtcatcc cgagcgacgc gagggatcta gttttcttcc ctcggctcga
      121 aatgacacct caaataaatg cattattctc caacaacgac gcagtcctgg gtaagaagag
      181 gaaaaaaacg gaggctcctc ggtgccgagg agccggaaat ctgctggaga ttttgtattt
      241 caacagaagc gcgtttcagt ccccacgcaa gccgtaaaac cgttccgcgt tgcgatagcg
      301 caccgcgtct ttgtctgctt tggtgagcct gccgttttcg tcgagctctt cgagctccgc
      361 cttgcaggtt tcgttgttga cctcgtgcgg aaagtccgac gagaagatga acggcttatt
      421 gccgacgacg ctcaccgcga agggcaacgt cagctcgtcg ccttcaacgc cgacgaaaat
      481 tcgatcctca tccacatggc gcctaatata gtctgtgatg gactccttgt cgcgcagctt
      541 caaaaagcgc ccgcgcggat cgtgttgtac gtggctgctc caggagcgct cgaagcgctc
      601 gacgcagttg atgaaccaac ccgatccggc ctccatgaag ccgaagcgaa cttttggatg
      661 tgtgtcgaag acgccactaa agagaatacc ggcgaagttg accatttgcc caaacgggtg
      721 ccccagcgca ttgacgggcg cataggggct catgtcatcc aatcccatat ggtcatgcac
      781 cccgccgtga atgccgatgc agcactccag acgattggcc tcttcataga ttggccagta
      841 gcgatcgtca cccagttgat tttgctgtcc tacggcgccc gtgctcggca gcatcgcacc
      901 gcagaagccg agctctttga cgatgcggcg tagctccttc accgcttcgg cgggttcttg
      961 caacgggatc aacccgagag cttgaaagcg agagctcttg ctcaggtact cgtcatacat
     1021 ccaatcatta tatgcccgcg ccagctcgac ggcccagtca cggctgacaa tacgaccgaa
     1081 cgacaaacca ctcgtcgggt agagcaccgc gctagccagg ccgacatctt ccatgaagtc
     1141 gatccaacct tctcttccga ctttaacgaa ggaaccgggc gggagaaaat gagcgttggc
     1201 ggcgtggaga tggtcattgg gcgggtaggg accacggccg actccaaaac ggtcgcgata
     1261 ttcatcgggc atccgcgtgc cgattgccgc gtgatcttcc accacatgcc cgtcgccatc
     1321 aattattttg tgtattttga tgctcacgtg ttgtctcctt tttcattccg gcttgcgaga
     1381 ggttagctgg taaaagcgct gcgagttgcg aaacaggatc gcttctttgt cgtcagccgt
     1441 gagttgggta ttctcgcgca gctcctccaa ctcatgtttg caggtttccg cgtcaacttc
     1501 gtgcgggtaa tcggtggaga aaacgaacgg cttgttgccc gagatgcgta ccgcctcagc
     1561 aagggtcagt tcttctcctt cgcagccgac gaagatgcgg ccttcatcga tatggcgaag
     1621 aatataatcg ataatctttt cccccttgcg cagttgcaag aaccggccgc gcggatcgtg
     1681 ttgtacgtgg ctttcccagg aaccggtgaa gcgctccatg caggtaagca gccaggccga
     1741 accggcttcc atgaagccga tgcgaacgcc gggaaacttg tcgaggattc cattaaaaat
     1801 gattccagca aaacaaatca tctggctcat cgggtggccg agcgcattga ccggtgcata
     1861 agggctgaga tcatccatca gcaatccttc gtgagctccg ccgtggatag cgatagcgca
     1921 gcccaatcgt tcggcctcgc cgtagatgga ccaatatttc tcagagccta aatgcggcat
     1981 attcgcaccg gtcgaaggca acacagcgcc gctaaagcct aagtcgcgca caatgcgtct
     2041 gagttcgatc accgcctccg cgggttcctg taacggaatc agccccatcg ctttaaagcg
     2101 gttgctcttc gataaatagg tatcgtaaat ccagttgttg taggcgcgcg caagctcgat
     2161 ggcccagtcg cggctgacga tcttgccgaa agcgaggccg gcgctggggt acaagaccgt
     2221 cgtgccgatg ccgacttctt cgagaaagag ctcccaacct tcgcgaccga cgttggcgaa
     2281 agcccctttc ggcgtgaaat ggcgattggc gctgtgcaga tgatcgatgg gcggaaacgg
     2341 gcttcttcca cgcgcgtccg aaaaacttct gccgacgtat tcctgcggca tgtatttcca
     2401 aatcgtcgag atgtcttcca ccacgtgtcc atcgccgtcg atgatttgat gccgggccga
     2461 tgtactcatt ttgtcctccc ttacttcctt agggaattat ttagatatag tttctttaca
     2521 ccgaaacggg gcaagacgtc cagatgatgg gtctctccgt tgccttcgcc ggatggagtt
     2581 tgacacttgg agatcctgat gttaggcacg gaagtcgagc gggatccttg gcgtgaggcc
     2641 agaatgattc gtcacgttca aatacttttc atcgtctggg gagtttcctt ggcgcttgca
     2701 gcgccgtcgc gttgtaacgc cgagatattc agaatcggat tcccgtcgct cgctaccggt
     2761 ttcgcgccct cgtgggtgac ggtggaaaaa gggatctgga aaaaacatgg acttgatgtc
     2821 gaattgatct atctgcgcgg cggttctaga acggtctcgg ctctcatcgg cggcagcgtc
     2881 gattttattc tgggctcgga cctcggtgtc acgacggcga ttctccaagg cgcgggcttg
     2941 actcgcgtcg gcgtcactac caacaccctt ggatactcga tggtgaccca gccgaatatc
     3001 aaaacggtgc gcgatctgaa aggcaaaatt atcggcatca cgccggggcg cgacgccgct
     3061 tacgcaaggg tcgtcaagtt gctgcgcgac aatggaatgg atgccagcaa agacgtcacg
     3121 tttttatcgg tgggcgacgg gggtccggcg agccgggttg cggctctatc gagcggcgtc
     3181 attcatgcga ccatgtttac tccgccctcg gatctgatct ccgagaaagc ggggatgaga
     3241 atcctcacca agatcgacgt cgcaaacgtc gggggcggtt tgaacactac cccgggcttc
     3301 atacagaaga gtagatctcg ggccttaaga tttctacgcg gctacatgga aggaattcag
     3361 tacatgaagt ctcacaagga tgagagtctg aagatattct ctaagtatgt acgcaatccc
     3421 gatctagcca tcatggctta tctttatgaa gagattgtc
//
LOCUS       DASSWZ010000182         5183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_840981, whole genome shotgun sequence.
ACCESSION   DASSWZ010000182 DASSWZ010000000
VERSION     DASSWZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840981"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..670)
                     /gene="rimI"
                     /locus_tag="VFN58_04485"
     CDS             complement(242..670)
                     /gene="rimI"
                     /locus_tag="VFN58_04485"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET8917282.1"
                     /translation="MVPADLDEVMAIERTSFRHPWSSNFFLEELQVACARSILAQVND
                     KIVGYVLFWLLPEAVDIHNIAVHIEFRRQRIGQALLQQVLEQARSRDSSRVTLEVRVS
                     NIPAQKLYESVGFVSQGLRKGYYSDDGEDAVVMALELAPR"
     gene            complement(1917..2141)
                     /locus_tag="VFN58_04490"
                     /pseudo
     CDS             complement(1917..2141)
                     /locus_tag="VFN58_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
     gene            2242..2317
                     /locus_tag="VFN58_04495"
     tRNA            2242..2317
                     /locus_tag="VFN58_04495"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2275..2277,aa:Cys,seq:gca)
     gene            complement(2350..2619)
                     /gene="grxC"
                     /locus_tag="VFN58_04500"
     CDS             complement(2350..2619)
                     /gene="grxC"
                     /locus_tag="VFN58_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HET8917283.1"
                     /translation="MAKVVIYTTRYCPYCFGAKALLRSKNIEFEEIDVTDDPAQRAEM
                     ERLSARRTVPQIFIDAHPIGGYDEARRLDAIGELDRLLRRSAETS"
     gene            2723..3313
                     /gene="coaE"
                     /locus_tag="VFN58_04505"
     CDS             2723..3313
                     /gene="coaE"
                     /locus_tag="VFN58_04505"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET8917284.1"
                     /translation="MVGLTGGIASGKSTVAAILRRLGAAIINADELSREVVQPEQGAW
                     EEIIKTFGPDILQEDKTLDRRKLRKIVFDNPEARKKLEAIIHPRVRALAERRISELAA
                     AGSSVIVYEVPLLFEAQIHLWLRPVILVACDLETQKKRLLERDHLTELEAQQHLDAQM
                     SLEEKRKLADYVIENDGTLEELEQGVRAVLQKIQAT"
     gene            complement(3306..3869)
                     /gene="efp"
                     /locus_tag="VFN58_04510"
     CDS             complement(3306..3869)
                     /gene="efp"
                     /locus_tag="VFN58_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET8917285.1"
                     /translation="MLIQATQLRPGMIIIHEGQLYRVFAVHHLTPGNKRGFMQTKMKN
                     LKSGVGTEYKFRSEDRVEQATLETRKMQYLYAEGDLHTFMDTENYEQITLTVEDIGDL
                     LSFLLPNEVVEVELYDSKPIGVSPPSTVDLQVVDTEPSMKGATASASYKPAKLETGVT
                     IQVPPFIQVGDKVRVDPSEGTYLERVK"
     gene            complement(3960..>5183)
                     /locus_tag="VFN58_04515"
     CDS             complement(3960..>5183)
                     /locus_tag="VFN58_04515"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET8917286.1"
                     /translation="MAVQQFDIAAAKLNVDANVASRLRRPDRALIVSVPIRMDDGKVQ
                     VFTGYRVQHNDVLGPFKGGIRYHPEVNLGEVSALAMWMTWKCSLVGLPLGGAKGGVTC
                     DPVELSRQELQSMTRRYTAEILNFIGPEIDIPAPDLGTNEQVMAWVMDTFSQHKGHAV
                     PEIVTGKPVDIGGTLGRREATGRGVVYTIIEAAKHLSIDLTRCTAVVQGFGNVGSVAV
                     KELANIGVKIIGVADRTGGFYDPKGLPVQKLLELADKYHSLENCPYGQKISSAEVLEL
                     KCDILVPAALEMQITKENADRLQCRILAEGANGPTTLEADAVLKEKGVFLIPDILANA
                     GGVVVSYFEWVQDLQNFFWTEQEVNQKLRDILVRAFREVLDMSQREKVDMRLAALMIG
                     IERVERAMLWRGLYA"
BASE COUNT         1206 a         1433 c         1269 g         1275 t
ORIGIN      
        1 cgggtgcaaa gtctattgaa cattcctcgt tccgttccac tggcgtaagg tccaaaaaaa
       61 atagcggcca agtatttcag accgccagag ggtaaaatga ctatacaaca gggcaaatta
      121 ttacgtcaag aggcccaaag aggactgccg cgggcaaatc aattacattt tcaatagttt
      181 agatatgaaa tatctaattc ttatctactt tggtcaccac ccagttattg acaagaacag
      241 ctcaccgagg agccagttcg agtgccataa ccacggcatc ctccccgtcg tctgagtaat
      301 aaccctttcg cagtccctgg ctcacaaatc caacggactc gtatagcttt tgtgcgggga
      361 tgtttgaaac ccttacttca agtgttactc gagatgagtc gcggctccgc gcctgctcta
      421 acacctgctg caaaagggct tgtccgattc gttgacggcg gaactcgatg tggacggcta
      481 tgttgtggat atcgactgcc tccggcagca accaaaaaag cacgtagccg acgattttgt
      541 cgttgacttg cgccaagatc gatcgagcac aggcgacttg aagctcttct agaaaaaaat
      601 tcgaactcca cggatgacga aatgatgttc tttcaatggc catgacctcg tccagatcgg
      661 ctggaaccat cgacctgaat gacacctttt gtccggaaac aaaaaaacca caggacgctt
      721 ttgattcctg tggctctgtc gtggagtcta ttcggaaatt ctttttgcta gcgatagctc
      781 gtctcccatt ggttcttttg accttttagc agcaagactt ccaatagttt attctcaaaa
      841 cgatgggcaa acccgacaac tctgtctttt agcggaatga tcgttgtaaa ctccggcgga
      901 aaggcgccga tgctggagga tattttgcga accctctcgt ccgtacatct tcctttgata
      961 ataaactgac tgtccttaag cgcaagatcg tagtcgtatt ccggcatttc gtcagacaat
     1021 tgctgccggt tgggaagacc gatgtcgggt aaccgccttg gaaactccaa tcgcaggaaa
     1081 agtgcaccac ctcgatcttc gaccgtatat acattgccat atcgccgttc gcgttcaagt
     1141 ttctcaacaa atcctttaga ataaaaccca tcaaccggaa tcggtatctc tctaattacg
     1201 ccggcgtgct ttgccaaaat ggggtgcaaa attattccga gcggagcgcc atagacagag
     1261 gccggaaata ccatctcttc cgccggcttg gcatgcaata tcccatcctt tagacgatag
     1321 actccctcgg ccaccgccag aaaaagcccg acgagaatat agttgttaat ttcttggctg
     1381 aagagaaccg ctggtccatg tacaataacc gccactatca tacaaaccgc cggataaatc
     1441 accgcgttat ggacgatatt caatccagtg ctgccggccg cggtaaacca cggactttga
     1501 acggcggctt cgatttgctt tttggtgtca tgcggcaaag cacccagcac tggctgcgcc
     1561 aagataacca gcgccttccc caacccttta gataaaaggc cgctgctcct gtaccggttc
     1621 gagagctctt cgaagctgtc tgacacttca ctcgggaagg tctttttcgg tctaatctcc
     1681 ttctttacca ggggtacggg ctttggtgcg gcgggcggcg ccaccggctt ggccgccccc
     1741 agacttgcaa ctggttttgg gggtggtacg gtcttttgtt ccgctggcgg tggtgtcggg
     1801 ttggctgggg acgccggttg ctgcattgca gcagcgggct tctgttcggc gttgactttt
     1861 cgaaagcgcg actgaaaatt ctcttcgaaa ttaacgtcct gatggagtgc ccgagcgcgc
     1921 gcgatcaaaa cctcttctgt ctcaaggttg ttcggatcag tcacgcagca gtccaccggg
     1981 cagactgccg cgcaggcctc gtaatcgtga aacccgacac actccgtgca taagagcgga
     2041 tcgatgacat acacagggtc tccctggcta atggcgttgt ttgggcattc cggctcgcat
     2101 gccccgcagt tgatacactc gctggtgatc atcgtcgcca ttgaagagac tcctgtttag
     2161 aattaattac ttgtttggtt ctgggttgtc aatgattcgt tgttgcaacg ctcggaaagt
     2221 tcatgttacc tatcgacata gggcggcgta cccaagtggt taagggagag gtctgcaaaa
     2281 cctcgatgca gcggttcgaa tccgctcgcc gcctccaacc ttacacggga atggtttgtc
     2341 catccactcc taactggttt cggcggatct tcgcagcaaa cgatccaatt ctccaatcgc
     2401 atcaaggcgc cgggcctcgt cgtaaccgcc gataggatgc gcatcgataa agatctgcgg
     2461 tacggttctc cgggcggaca gacgctccat ttcggctctc tgtgctgggt catcggtgac
     2521 gtcgatttct tcaaactcaa tgttctttga gcgcagcagc gccttggcgc caaaacagta
     2581 aggacaatag cgggttgtat agataacgac ttttgccatt ctcgtcggag ccccgttagc
     2641 ttaataatcg gatatctcgg tcattggctt taagatcttt cttgaaacaa cagtcctttt
     2701 ttgccattag tgggcggtga aattggtagg tctaaccggc ggaatcgcat cgggcaaaag
     2761 tacagttgcc gcaatcttgc gccgtcttgg cgcggcgatt atcaacgccg atgaattatc
     2821 ccgcgaggtc gtgcaacccg aacaaggtgc gtgggaggaa atcattaaga cctttggtcc
     2881 tgatattcta caggaagaca agactctaga ccgcagaaaa ctaaggaaaa tcgtcttcga
     2941 caaccccgaa gcacgaaaga aacttgaagc gatcatccat ccaagggtcc gtgctctggc
     3001 ggagagaaga atcagcgagc tagcggcggc cggtagttca gtcattgtct atgaagtgcc
     3061 actgcttttc gaggctcaaa tccatctctg gcttcgcccc gtgatcttgg ttgcctgcga
     3121 ccttgaaacg caaaagaaaa ggttgctcga acgagatcat ttgacggaac tcgaagctca
     3181 acagcacctc gatgcgcaga tgagcttgga ggaaaaacgc aaactggcgg actatgtcat
     3241 cgagaacgac ggtaccctgg aggaacttga acaaggtgtt cgcgcggtat tgcagaagat
     3301 ccaggctact tgacccgctc cagatacgtt ccttcggaag gatccactct aactttatcc
     3361 cccacctgga tgaatggtgg aacttgaatc gtgaccccag tttccagctt tgccggttta
     3421 taagaggcgc tggccgtcgc ccctttcatc gacggttccg tatcgacgac ctgaaggtca
     3481 accgtcgagg gcgggctgac accgataggc ttgctgtcgt acaactccac ttcgacgact
     3541 tcgttgggca agagaaaaga caacaggtca ccgatatctt ccaccgtcaa ggtaatctgt
     3601 tcatagttct ccgtgtccat gaatgtgtgc aggtcgcctt cggcgtagag atactgcatc
     3661 tttcgagtct ctaacgtagc ctgttcgacg cggtcctcgg accggaactt atattcggtc
     3721 cccacgccgg atttgagatt cttcatcttc gtctgcatga atccgcgttt gttgccgggg
     3781 gtcaaatggt gaactgcaaa tacccggtaa agttgacctt catgaatgat gatcatgccg
     3841 ggtctgagct gtgtcgcctg gatcaacatc tagattcctt cctgagaatt ctctctctgt
     3901 tgttagcagt ttatctcggt agcttcaacg cgcttcggct gccattcgcg cttcacgctt
     3961 caggcgtaaa gaccgcgcca gagcatcgct cgctcgaccc gctcgatacc gatcatcaag
     4021 gcggccaatc gcatgtccac cttttcccgc tggctcatat cgagcacctc gcggaaggcc
     4081 cggaccagga tgtctcgtag tttctgattc acttcttgct cggtccagaa aaaattctgt
     4141 agatcctgga cccactcgaa ataggacacc acgacaccac ccgcattggc cagaatatca
     4201 ggaatgagga aaaccccctt ttccttaaga acggcatcgg cctccaacgt cgttggacca
     4261 ttggcgccct cggctaaaat ccggcattgc agccggtcag cgttttcttt ggtaatctgc
     4321 atctccagtg ccgccggaac aagaatatcg cacttcaact ccaaaacctc cgcgctactt
     4381 atcttctgcc cgtacgggca gttttcaaga ctgtggtatt tgtcggccag ctcgagaagc
     4441 ttttgcaccg gaagtccttt cgggtcgtaa aagccaccgg tccggtcggc tacgccgata
     4501 atcttcacac ctatattggc gagttctttc accgctaccg agcctacatt gccgaaccct
     4561 tgcaccaccg ccgtgcatcg ggtcagatct atacttaagt gtttcgccgc ttcaatgatc
     4621 gtgtagacca cgcctcgccc tgtcgcttcg cgtctaccca gcgtacctcc aatgtctacc
     4681 ggtttaccgg taacgatttc cggtacggca tggcccttgt gctgtgaaaa agtgtccatg
     4741 acccatgcca taacttgttc gttggttccc aggtcaggtg cagggatatc gatttcgggt
     4801 ccaataaaat taagaatctc cgccgtatag cgccgcgtca tggactgtaa ctcctgtcgt
     4861 gaaagctcca ccggatcgca tgtaacgcct ccctttgccc cgcctaaagg caaacccaca
     4921 agcgagcact tccacgtcat ccacatggcc agagcggaaa cctccccgag atttacctcc
     4981 gggtgataac gaatacctcc cttaaacggt cccagcacgt cgttgtgctg tacccgataa
     5041 ccggtaaata cctggacctt accgtcatcc attcgaatag gcacactgac aatcaatgct
     5101 cggtccggcc gccgcaatcg actcgcgacg ttcgcgtcga cattgagctt ggccgcggcg
     5161 atatcgaact gctggaccgc cat
//
LOCUS       DASSWZ010000183         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1237370, whole genome shotgun sequence.
ACCESSION   DASSWZ010000183 DASSWZ010000000
VERSION     DASSWZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237370"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..2427)
                     /locus_tag="VFN58_04520"
     CDS             complement(40..2427)
                     /locus_tag="VFN58_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET8917287.1"
                     /translation="MAIQRLHTYCAMCISRCGVVATVEDGRLTMVNADPAHPNGCICV
                     KGTAAPEIVYSPDRLRSPMKRTRPKGDPDPGWAPISWDEALELTASCLLAVKARFGPE
                     AVVFAHGTPSGSATSDSVPWLQRLANAFGSPNLMSSIHICNWNRTYGSQYTYGVPTPA
                     PDYDNSRCILLWGFNPHASWPAAAARISQAKARGAQVIVVDPRRSGVGDKADLWLKVR
                     PGGDVALALSMIHVLLEENLYDREFVRDWTNGAFLVREDNNQLLTAGDLSHSGDKDTY
                     FVSNDCNGELVSYRPEQGYGRDGVLPGLKGNHNVTLSDGRVVGCRPSFDRLAELAAQF
                     APERVEHSTWVAAHEVRRTARMFAAEKPSCYYSWAGIEQHDNATGMNRALCIFFALTG
                     QFDRRGSNLLYTTTSIQPITGQELLPKPMTARRLGMNEFPLGSPGHSGRVQSKHVYEA
                     ILTDRPYPVKAMISFGGDPLLGNGDGQRGKAALEALDFFVHVDMFANPSAACADLLLP
                     ASTCWEHEGLMSSFPIAEETANWAQLRPPVVQSLHESRSDLEIIFDLATRLGLGEHFF
                     GGDIDAALNYHLAPSGLTVQQLRANRMGMRAAGKTRYQKYAEIDARTGRSKGFQTPTR
                     KIEIYSTRFAASGYPPLPGMREAGDGHGRLGDTSDEYPLILTSFRLVQFCDQQHRNIP
                     RLRRQSREPLLEINPETAASLSVQADEWIRLETAMGGIRLKAKLNSCLHPKVVATQYG
                     WWQACNELGLPGYDAFGPRGANVNLLISNDATDPISGSVPHRSQPCRVRKENGAA"
BASE COUNT          616 a          777 c          816 g          603 t
ORIGIN      
        1 ggctagaagc ctacgcaaat gcgatctctt agagatttgt tatgccgcgc cgttttcctt
       61 tctgacccgg cacggctggg aacgatgcgg caccgaaccg ctgatgggat cggtggcatc
      121 gttgctgatg agcaggttaa cgttggctcc ccgcgggcca aaagcgtcgt aaccgggaag
      181 ccccagttcg ttacaggctt gccaccaacc gtattgagtc gccacgactt tgggatgaag
      241 acaagagttc aacttcgcct tgagtcgaat accgcccatg gctgtctcta acctgatcca
      301 ttcgtcagct tgaacgctca aagaggcggc ggtttccgga ttaatctcca aaagcggttc
      361 acgggactgc cggcgcaggc gagggatgtt gcggtgttgc tgatcacaga attgcaccag
      421 cctaaaggag gtgagaatca gcggatattc gtctgatgtg tcgcccagac gtccgtggcc
      481 atcacctgcc tccctcatcc ctggcagcgg gggataaccg gatgcagcaa atcttgtcga
      541 gtaaatctcg atcttccgcg ttggggtttg gaaacctttt gatcttcccg tccgtgcatc
      601 gatctcagca tatttttgat atcgggtttt accagcggcg cgcataccca tccgattggc
      661 gcgcaattgt tgaacggtga ggcccgaggg cgcgaggtga tagttcaaag ccgcatcgat
      721 atcgccgccg aaaaagtgtt cacccaagcc caaccgggta gccagatcaa aaatgatttc
      781 cagatcggag cgcgactcat gcagcgactg cacgactggc ggcctcagct gggcccagtt
      841 ggcggtctcc tcggcgatag gaaaagatga catgagaccc tcgtgctccc agcaggtgga
      901 agccgggagc aacaggtcgg cgcaagcagc gctgggattg gcaaacatgt ccacgtggac
      961 gaagaaatcc agagcctcga gcgcagcctt gccgcgctgg ccatcaccgt tgcccaaaag
     1021 cgggtcgcca ccgaaactga tcatcgcctt taccggataa ggtcggtcgg tcaaaatggc
     1081 ttcgtagaca tgtttggatt gaacgcgacc tgagtgtccg ggcgagccaa gcgggaactc
     1141 gttcatgcca agacggcgcg cggtcattgg cttcggtagc agctcttggc ctgtgatcgg
     1201 ttggatggat gtggtggtgt agaggagatt gcttccacgt cgatcgaact gccccgtcag
     1261 cgcaaaaaaa atacacaagg cgcgattcat tccggtggca ttgtcatgtt gttcaatgcc
     1321 ggcccagctg tagtaacaag agggtttttc cgcggcgaac atgcgcgccg tgcgccgaac
     1381 ttcatgcgcc gccacccaag ttgaatgttc cactcgctcc ggcgcgaact gtgctgcaag
     1441 ctctgcaagc ctgtcgaatg acggtcggca tccaaccacg cggccgtccg atagggtgac
     1501 gttatggttg cccttgagtc cagggaggac gccgtctcga ccgtagccct gctcggggcg
     1561 atagctcact agctctccgt tgcaatcatt agatacgaaa tacgtatcct tgtcgcccga
     1621 atgcgaaagg tctcctgcgg taagtagttg attgttgtct tctcgcacca ggaaagcgcc
     1681 gttggtccag tcgcgcacga attcacggtc atagagattt tcctccaaca gcacgtgaat
     1741 catgctcaag gccagcgcca cgtcgccgcc aggtcggacc ttaagccaga gatccgcttt
     1801 atcgccgact ccggatctac gcggatcgac gacgatgact tgggctccac gcgccttggc
     1861 ttggctgatg cgcgcggcag cggcgggcca tgatgcgtga ggattgaagc cccaaaggag
     1921 aatacagcgc gagttatcgt agtccggcgc cggagttggc acgccgtaag tatactgaga
     1981 cccataggtc ctattccagt tgcagatgtg tatggagctc atcaggttgg ggctgccgaa
     2041 ggcgttggcc aagcgttgaa gccaaggcac ggagtcggaa gtggcgcttc ctgagggagt
     2101 tccatgcgca aagaccactg cttcagggcc gaatctcgct ttcacggcca aaaggcagga
     2161 ggcggttaac tccagagcct cgtcccagga gatcggcgcc caaccggggt cggggtcccc
     2221 tttgggtcgg gtacgtttca tgggagagcg gaggcggtca ggagaataga caatctcggg
     2281 cgcggccgtt cccttgacgc agatacagcc gttcggatgc gcgggatcgg cattgaccat
     2341 agtcaagcgg ccgtcctcga cggtagccac tacgccgcat ctggaaatgc acatggcgca
     2401 gtaagtgtga agccgctgga tagccatgaa agtgactatg gtcgcatcat ttgatcggac
     2461 agttacccac tacgaagatc ccagtgcggc tatgcccaac caggagaaaa atggatatca
     2521 ccacagagcg cgcgaagagc acaaagaaaa catgtttaag attcgacgcc ctctctttta
     2581 tccgatcctc gtgaccttcg cgaactccgt cgtgaagttg attgccctag acaaaccttc
     2641 agctaacgaa ctctcactgg aaaagtttcc gaaaggtctc gttaacttca gcctcttttt
     2701 gacccaagtc gacatcggtc atgagcagct tgtgttgctt gagtatcttg ccgtatttcg
     2761 attcgacgtc gtcgcgaatc ggcactcgac cgtattggtt cacgacttcc tg
//
LOCUS       DASSWZ010000184         5462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_430783, whole genome shotgun sequence.
ACCESSION   DASSWZ010000184 DASSWZ010000000
VERSION     DASSWZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430783"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFN58_04525"
     CDS             complement(<1..206)
                     /locus_tag="VFN58_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8917288.1"
                     /translation="MAKIIENLVEYMLAARQAKLPGEVVQKGKSHTLDSLAAIVSGST
                     LKPGKLGLQHAREQGGKEECSVLGS"
     gene            410..1948
                     /locus_tag="VFN58_04530"
     CDS             410..1948
                     /locus_tag="VFN58_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917289.1"
                     /translation="MSIDVRSLSRREFLEKAAAAGAVISLSPLARLSYAQSPIRNRIT
                     VAHGVGVYSLNPYAVNTSPLQAVWGSVMEPLIDADYDKRGYQGVLAESWQMKGARLQF
                     KLRNGVRFHDGTPFSSKDVVASFKRILTDKQSLQAPNLGNIKEMDAPDDLTVVLTLKK
                     ADANALEDINSRLIMKQSVAEKMGEADNPPIGTGPFKFVSWDRSGQFVMRRNENYWGQ
                     APKIDEVIYKNIQEDAARIAALEAGQADIVSNLPPHEVERLKSNPRLRVQPVQGLRPI
                     FLVLSPAYKPLDNPKVRRAISHAIDRDRIIKLVLEGNAYPLRGLLGPQVFGYDPEARA
                     YPYDPEKAKQLLNEAGFANGFEIEYHSPTGRYPKDREVAQVIVEQLSKVGIKANLKTP
                     EWSIFNTDYKNGKYAFYLTGRGSLTDADTLFQQYFRTGMTKRVLGYSNPKLDEILDAQ
                     QQTFDVKKREKLLWEAHRLILEDAPAIPLWNAMDIYAHRADLVWTAPPDEMVQLKQAY
                     FKTR"
     gene            2239..3300
                     /locus_tag="VFN58_04535"
     CDS             2239..3300
                     /locus_tag="VFN58_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917290.1"
                     /translation="MQRIDITVSAVRAWIAVAALFLSGCAAKSLSPANETMPPIVLAT
                     LGDAGIKDVRHLYRAAVCRQLPTGSLPCEELILRFPNEAAAGSLSRQTDIKDHYQIAF
                     VPGFFSDCLNGIFYPFTDVIADLTGLGFVVHNLPTFGRASSAANAERLATQLAQLSPD
                     PKPFIMVVYSKGLPDVLELLLRYPLMSQRIAAIISIAGAANGSPIADEHYEIYRDWLA
                     GLPIPGCDRGTGEEVRDLRRDVRLQWWAQHRSAITVPIFSIVAVPKTDRVSPILKQTY
                     EKLAGIDTRNDGLLLWYDTIVTGGYLLGYVNADHLAIIVPASQQVPALYFLFKDDVPR
                     AAIVRAAIEVVAETLETSR"
     gene            3429..4385
                     /locus_tag="VFN58_04540"
     CDS             3429..4385
                     /locus_tag="VFN58_04540"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET8917291.1"
                     /translation="MLPARVVSTLLGAFGSFGLAFVTEAYPSEPTKAAMQAPTLKEIK
                     NASYSGIEGLKGSVKLVDGKWKGRPYKKGSASRPVVSLIGDFRITGDLDGDGTDDAVV
                     LLNYAPGGTGQLLHLALMARKKGKIQNLATALIGDRVQIRDVRIEQKRIFVDVIQAGP
                     KDAMCCPGEVTTREWTLEPGGKLNRYTVTAKPARLTLETIGNTEWILRSWDSKQPAPT
                     RPAVTLVFKDGRFTGSSGCNNYFAPAKDGKIPGDVEVGAVGTTRKSCPDNEMSVERRF
                     LEQLVRVRKFGFLVTQLALSWEKDGVWKTMLFDKRQPRLTDK"
     gene            4620..>5462
                     /locus_tag="VFN58_04545"
     CDS             4620..>5462
                     /locus_tag="VFN58_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET8917292.1"
                     /translation="MAETDLERKPRPVKWLPYVVGVLTAGVILLAMFVVDQLEQQRFD
                     RIREARVLRSLSNARARLEAELNQRLFLTRGLKAYVSTHPNISVPEFNHLASVLISQH
                     GTSGIRSIQLAKDTVVSHVYPVKGNKQAIGLDLLNVPEQSSAVQRALDSRATVVAGPV
                     ELVQGGTAFIGRTPIYLTPPGGPPNSGAYWGLTTILIDDAAIFSEAGISQNTDGLQFA
                     LRGRDGMGAKGEAFFGDPGVFDSHPMVLDIEIPGGTWQLAAVPLPGVLSASPNLWLLR
                     LGGGL"
BASE COUNT         1258 a         1479 c         1476 g         1249 t
ORIGIN      
        1 gatcccaaga cgctgcactc ttccttgccg ccctgctcgc gcgcgtgttg cagcccgagc
       61 tttcccggct tcaaggtcga acccgatacg atcgcggcca gggaatcgag agtgtggctc
      121 ttgccttttt gcacgacttc ccccggcaat ttcgcctgcc tcgcggcgag catatactcc
      181 accaagttct cgatgatttt cgccatggtt cctccagcca aaagttgcga tcaatccact
      241 gttccatcat tccaatcttt agtactccat ctttgtacgc tagatcgccc attgagaaca
      301 agttgttgac actatttgct tagctgagtt atacggcgta ctgatagcgg tattgtccac
      361 tccatatatt ctgaacttga cgtggtcttc cagctcaacg gaggttcctg tgagtattga
      421 cgttagaagc ctaagtcgga gagagtttct ggaaaaagcc gcggctgccg gcgcggtcat
      481 ttctttatcg cctttggcgc gattgtctta tgcacaatcg ccgattagga atcgcatcac
      541 cgtcgcgcac ggcgtcggcg tttattcttt gaatccctac gccgtcaaca cctccccatt
      601 acaggccgtc tggggctcgg tgatggaacc gctgatcgac gctgactacg acaagcgggg
      661 ataccagggc gtactggcag aatcctggca gatgaaaggc gccagattgc aattcaaact
      721 ccgcaatggt gttcgctttc acgatggcac gccgtttagc tccaaagacg tcgtcgcctc
      781 ctttaagcgg atcctcaccg acaaacagag cttgcaggca ccgaaccttg ggaacattaa
      841 agaaatggac gctcccgatg acctcaccgt cgtcctgacc ctcaaaaaag cggatgccaa
      901 cgcattggag gatataaata gccggttaat catgaaacag tcggtcgcgg aaaaaatggg
      961 cgaggctgat aacccgccga tcgggacagg accgtttaag ttcgttagct gggaccgctc
     1021 gggccagttt gttatgcggc ggaacgagaa ttattggggc caggcgccaa aaatcgatga
     1081 agttatttac aaaaatattc aagaagacgc tgcgcggatt gcagcgctcg aagccggcca
     1141 ggcggatatc gtcagcaacc tgccgccgca tgaagtggag cgacttaaat ctaacccgcg
     1201 cctgcgcgtg cagccggtgc agggtctgcg gccgattttt ttggttctga gtccggccta
     1261 taagccgttg gacaatccga aggttcgcag ggctatcagt cacgccatcg atcgtgaccg
     1321 gatcattaaa cttgtgctgg aaggaaacgc ctatccgttg agaggcctgc tcggtccgca
     1381 ggtattcggc tacgaccccg aagcgagagc ttatccgtac gacccagaga aggcgaaaca
     1441 gcttcttaac gaggccggct tcgccaacgg ctttgagatc gaataccaca gccccaccgg
     1501 ccgatacccc aaggatcgcg aagtcgcgca agtgatcgtc gaacagcttt ccaaggtcgg
     1561 tatcaaagcg aatctcaaaa ccccggagtg gagtatcttc aacaccgact ataagaacgg
     1621 caagtatgct ttttacctga ctggaagagg tagcctcacg gacgctgata cgctttttca
     1681 acaatacttt cggaccggca tgaccaagcg agtccttggt tacagcaatc ccaaactcga
     1741 cgagatccta gatgcccagc agcaaacgtt cgacgtgaaa aaaagagaaa aactgctttg
     1801 ggaagcgcac cgactcatcc tggaggacgc gccggcgata ccattgtgga acgccatgga
     1861 catctacgcc caccgcgccg acctcgtctg gactgcgccg ccggacgaaa tggtccagtt
     1921 aaagcaggcc tatttcaaaa cgcggtagag cgctcaattt tttggatctt cagtagttta
     1981 cccagtgtcc ggaacctttc tggcaatctt atccttggcc attgttccag cacagacatg
     2041 tagcgcaaat cgagcacgcc tcgtcttttg agggatgttt aaaaggtata gtctgctgag
     2101 taagaaaaga ggagaagtct gtttttcgac tgcgttacaa cgcgacaagg acaacttcga
     2161 tctcattcct gcgaatcgtg ttcaaacagg cagcgaagca gaacgtttag ccgccgactt
     2221 tttcaaccga ggttaccgtt gcaacggatt gacatcaccg tatcggcagt ccgggcatgg
     2281 atcgccgttg ccgctctgtt tctctccggc tgcgctgcga aatctttgtc gccggcaaat
     2341 gaaaccatgc cgccgatcgt tctcgcgacc ctcggcgacg ccggcattaa ggatgtccgc
     2401 cacctttatc gagccgccgt ttgcaggcag cttccaaccg gctctctgcc ctgcgaggag
     2461 ttgattttgc ggtttccaaa cgaagccgct gctggcagcc tctcaagaca aacagacatc
     2521 aaggatcact accagatcgc gtttgtcccg ggattcttca gcgactgttt gaacgggatc
     2581 ttctatccat tcacagatgt tatcgccgat ttaacggggt taggattcgt cgtccataat
     2641 ttgccgactt tcggccgcgc aagctcggca gcgaatgcag agcggctggc cacgcaattg
     2701 gcccagctca gcccggaccc caagccgttt atcatggtcg tctattcgaa gggacttccg
     2761 gacgtgctcg aattgctgct tcgctaccct ctgatgtcgc agcgcatcgc ggctatcata
     2821 agcatagctg gggcagccaa cgggagcccg atcgccgatg aacactatga gatttatcgc
     2881 gactggctcg cggggctccc gatacccggc tgcgaccgtg gcaccggcga agaagtccga
     2941 gacttgcgcc gggatgtccg cttgcaatgg tgggcgcagc atcgctccgc cattacggtg
     3001 ccgatatttt ccatcgtagc ggtaccgaaa accgaccggg tttcaccgat actgaaacaa
     3061 acttacgaga aacttgccgg aatcgatacc cgcaatgatg gcttgctttt atggtacgat
     3121 actattgtaa cgggcggcta cttactggga tatgtgaacg ccgatcacct ggccattatt
     3181 gttcctgcaa gccaacaggt tccagcgctt tacttcttgt ttaaagacga tgttccacgc
     3241 gctgcaatcg taagagcggc catcgaagtt gtcgctgaaa cgctcgaaac tagccggtaa
     3301 acactgcgtc gccgaaggaa taaggatgca accttaactt cccgcgatta gaaaaagatc
     3361 tagctcagcg aggcaatatt tagtttcgcg tcgtttatcc tcctgaaaaa agtggtataa
     3421 attcagccat gttgccggcg agggttgtca gcacacttct cggtgctttt ggatcgttcg
     3481 gtctggcgtt tgtgaccgaa gcctacccat ccgagccgac gaaggcagca atgcaggctc
     3541 cgactctcaa ggaaattaag aacgcctcat actccggcat tgaaggcttg aagggttccg
     3601 tcaagctcgt tgatgggaaa tggaaaggta gaccttataa gaagggcagc gcctcccggc
     3661 ccgtcgtcag tttaattggt gatttccgga tcaccggtga tttagatggc gatggtacag
     3721 acgacgcggt ggttttgctg aactacgcgc cgggtgggac cggtcagtta cttcacctgg
     3781 ccttgatggc tcgcaagaag ggcaaaatcc aaaaccttgc gaccgctttg atcggagaca
     3841 gagtgcagat ccgcgatgtt cgtatcgaac aaaagcgaat cttcgtggat gtcatacagg
     3901 ccggcccgaa agacgccatg tgctgtcctg gagaagtaac gacccgtgag tggactcttg
     3961 aaccgggcgg caagctaaat cgttacaccg tgaccgccaa accggcgcgc ttgaccctcg
     4021 agacgatcgg gaacacggaa tggattctgc gatcttggga ttctaagcaa ccggctccga
     4081 ctcggcccgc cgtaactctt gttttcaaag acgggcgttt taccggcagc agcggctgta
     4141 acaattattt cgcgccggca aaagatggca agataccggg ggatgtggaa gtgggagcag
     4201 tggggacaac gcgcaaatct tgcccggaca acgagatgtc cgtcgagcgt cggtttctcg
     4261 agcaactagt tcgcgtaaga aaattcggat ttctggtcac ccaactcgcg ttatcctggg
     4321 agaaagatgg cgtctggaaa accatgctct tcgacaagcg gcaacctcgc ctcaccgaca
     4381 agtaatgaac tcaccacttg ctactcgtta cagtctaccg cagctcacaa tcggaaacat
     4441 cctgtctttc cgcttcaaaa tttccggagt gacggttccg aagcttttgc ttttttgacg
     4501 tggatttatg aggccgaccg atctaagatt ggagcccgac aagtttgccc gtacgaagca
     4561 aagagaatcg tttgagcgtt acgtgccgcg cgcaggaagg agagtggtgc aaaactccca
     4621 tggcggaaac tgacttagaa agaaaaccga gaccggttaa gtggctaccc tatgtggttg
     4681 gggtcctaac cgccggcgta attctgctgg cgatgtttgt ggttgatcag ctggagcagc
     4741 agcgcttcga tagaattcgg gaggctcggg tcttacgtag cctcagcaat gcccgcgcgc
     4801 gcttggaagc tgagctcaac cagcggctgt ttctgacgcg agggctcaag gcttacgtgt
     4861 cgactcatcc caatattagt gtgccggagt ttaatcatct cgctagcgta ctcatatccc
     4921 agcatggcac ttcgggcatt cgcagcattc agttggcgaa ggacactgtt gtgagccacg
     4981 tttatcccgt caaaggaaat aaacaagcga tagggttaga cctactaaat gtgcctgaac
     5041 agagttcggc ggtgcaacgc gcgcttgata gccgggcaac cgttgttgct ggtccggtgg
     5101 agcttgtgca aggagggacg gcatttattg gccgcactcc aatttatctg acgcctcccg
     5161 ggggaccacc taacagcggt gcctattggg gccttactac gattctcatt gacgatgcgg
     5221 ccattttctc ggaagcaggc atctcgcaaa ataccgatgg tcttcagttc gctttgcgtg
     5281 gaagagacgg gatgggcgcc aaaggcgaag cctttttcgg cgacccgggt gtctttgatt
     5341 cgcatcccat ggttctcgac atcgagattc ccggcggcac atggcagttg gcggcggttc
     5401 ctctgcctgg tgtgctgtcg gcttctccca acctctggct cttgcggctc ggcggcggat
     5461 tg
//
LOCUS       DASSWZ010000185         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_289346, whole genome shotgun sequence.
ACCESSION   DASSWZ010000185 DASSWZ010000000
VERSION     DASSWZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289346"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..893
                     /locus_tag="VFN58_04550"
     CDS             141..893
                     /locus_tag="VFN58_04550"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET8917293.1"
                     /translation="MKGKVALVTGGSSGIGRATALAFAREGARVAIVSRNKNKATEAV
                     QSIKKLGGEVIWIQTDVSNAAQVRQMVHQTVARFGRIDYAFNNGGSGGNGRRTADMDE
                     RDWDKTIDGYLKSVWLCMKYEILAMLKRGAGVIVNNSSVDGVRAYPFPVGSAYSAAKH
                     GVIGLTKSAALEYVSHGVRINAVCPGWVRTPPVERWMKRDPKVARQIIAQEPIGRLGT
                     PEEIAEMVIWLCSERATFVIGAALAIDGGYLA"
     gene            1381..1623
                     /locus_tag="VFN58_04555"
     CDS             1381..1623
                     /locus_tag="VFN58_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917294.1"
                     /translation="MDKDILICLQALEWIRLEPHLPSDVCERLTEDDRGMPMLEGRIV
                     PDDATYQRILDIARTHCPEIVKHIEKERQKSLTESG"
BASE COUNT          465 a          542 c          585 g          487 t
ORIGIN      
        1 tttgcgctat gtgagctagc gcatcaatcc aaccatttat ccctcccatc cctactcccg
       61 caatcgttac gaagcttgat tactttcagg ctttggggat aaaacgcagt gggtttggca
      121 gcgtggaaga cggactgcat atgaaaggca aagttgcgct ggtgactggc ggtagttccg
      181 gtatcggacg ggccactgca ttggctttcg ccagagaagg tgcacgggtg gccattgtga
      241 gccgcaataa gaacaaagcc accgaagctg ttcaatctat caaaaaactt ggcggcgagg
      301 tcatctggat tcagacggac gtcagcaacg ccgcacaagt gcggcagatg gtgcatcaaa
      361 cggtggccag gtttggtcgc atcgactatg ccttcaacaa tggtggtagc ggcggcaacg
      421 ggcggcggac cgcggacatg gacgagcgtg attgggacaa gacgattgac ggctatctca
      481 agagcgtgtg gttatgcatg aagtacgaga tcctcgcgat gctcaagcgg ggcgcggggg
      541 tcatcgtgaa caactcctca gtcgatggag tacgggctta tccgtttcca gtcgggtcag
      601 cgtattcagc agcgaaacat ggcgtcatcg gattgaccaa gtccgccgcg ttggagtatg
      661 tctctcacgg agttcgcatc aatgcggtgt gcccgggctg ggtccggaca ccgcctgtcg
      721 agcgctggat gaagcgtgac ccgaaggttg cgcggcagat catcgcccag gaaccaattg
      781 gacgcttggg gacgcctgag gaaattgccg agatggtcat ctggctgtgt tccgagcgag
      841 ccactttcgt catcggcgcg gcgctcgcga tcgatggcgg ctacttggca tagacacgct
      901 gtgtcgcgca tcgagtcagc ctaacccgca gtggagcaga gcaccgcaag cgcagttgcg
      961 gttcccacgg agcggcgtgt ctcggcggct gctcaccgcg agcgttaacc gtgtaattga
     1021 acctgatttc tccaacgaac caatcttacg ccgcatccga ctgaggcgtc gttcagttca
     1081 atcttaggca aaaaatctcg tttccccctc gactaatccc tgtcgccttc agccgcgtaa
     1141 atcacacttt cctttatttc tatccgcgtg atagataacg ctcgaaacga ggtgcgcaat
     1201 gaagaaggct gcggtgccat caatccttgt tgccgtggtg ttgcttgctg ttgcggtcat
     1261 agccgaggtg caacagccga agaaagtttc gcggataggt ttctagctgc ttcctctcct
     1321 tcctttttct cgactccttt tccttgtttt tgagaacgaa taggactagc ttcatgtccc
     1381 atggacaaag acattctcat ctgcttacag gccttggagt ggatacgttt ggaaccgcat
     1441 cttccgagcg atgtgtgcga gagattgact gaagacgacc gcggcatgcc gatgctcgag
     1501 ggccgcatag ttcccgatga tgcgacttac cagagaattc tagatatagc gcggacacat
     1561 tgccctgaga ttgtgaaaca catcgagaag gaaaggcaaa aatccctgac ggaatccggg
     1621 tgagccgccg ccggagaggc agacctaaat cagtcgtccc ttcattgcgg caacacgcgc
     1681 tctcaatgct tcgagttccg ctttaacttc ctcgtcatga gttttcgcga actcaaggcc
     1741 taattcatca atacgctttt cgagttcttc ggcgttcatt tggggtggcg tagtttgttc
     1801 ccatccggtg ctaatgacaa gagggccgcc cagaccgaag cccgcttaca aagtgtagga
     1861 gagatccaag cccccgatct cagcaatggg acgattgtag caaggcgctt gcagaaatct
     1921 cactatttgg gcgcagaaac cgtggtcatc gaccgtcaaa cagcctatgt agtttgagtc
     1981 ttcatggtcg ataaaaagaa aacatgtggt gctcggttga atattggact gtgctaccgc
     2041 cggaaaatgc cgatttcgcc ttttggctgt ttgtcttca
//
LOCUS       DASSWZ010000186         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_416725, whole genome shotgun sequence.
ACCESSION   DASSWZ010000186 DASSWZ010000000
VERSION     DASSWZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416725"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..201)
                     /locus_tag="VFN58_04560"
     CDS             complement(1..201)
                     /locus_tag="VFN58_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917295.1"
                     /translation="METTLPLELIEIQYEDATYHAAVCHRCGTKIYPAEQLEPHLDRH
                     QLKDLYLEGELKKLQFAMARMR"
     gene            614..1756
                     /locus_tag="VFN58_04565"
     CDS             614..1756
                     /locus_tag="VFN58_04565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8917296.1"
                     /translation="MVTAIDNTLPTVVFSEEKSGRFKVGYVALSNPRALNALDLGMLR
                     VMEGKLLEWWQDDDLACVVLYAESEKAFCAGGDVKSLVMALQREGTIAAARDYFTTEY
                     FVDYLVHVYPKPILCWADGITMGGGIGIMSGASSRVVTERTVMAMPEIAIGLFPDVGG
                     TYFLNQLPAGVGLFLGLTGARISGDDATSVGMADVIVRSEKRGEIFAGLSRLSWSPDP
                     QKNKQTLRDYLASVAEPDPANRGDLLKRLDIVKKLTLKATVEEIDSGFRSWHGNDEWI
                     ESGIQGYLSGSPTSAKAIFQQLTSGKELSLKQAFLREWDMALNFCERSDFREGVRSRL
                     IDKDQKPQWNPPTLAGIRDEDIERFFSKQHGQPDLLAQKLSELALG"
     gene            1843..2817
                     /locus_tag="VFN58_04570"
     CDS             1843..2817
                     /locus_tag="VFN58_04570"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917297.1"
                     /translation="MTINELLEIEFPVMRYDITMPLLEGRVAIDGVKLKPVKSSSMIN
                     KEDPKMIAGEFGLCDLNIGYWLPAIEAGWEIVGLPVFSKRKPVYQLIFTHADSGIRSP
                     KDLAGKKVGSRTYRTALTVWARGLLQDRYGVDFSKVQWVLQAKEVFPVHDPAAKIEYV
                     DDKKNMVERLIGGELDAIITDISDTKLFQILETHPKVERLFPDYIGEDQKLYRETGLY
                     TPVHMIVMSRKLDRERPDLAAKFYTAFEQAKRIAYDDILSDRGGFSVVYLREKLKEQI
                     AAWDDPWKYGLKANKSTIDAFIKYNIEQGMVRSQFFYGDLFAAGTLDT"
     gene            2841..4127
                     /locus_tag="VFN58_04575"
     CDS             2841..4127
                     /locus_tag="VFN58_04575"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8917298.1"
                     /translation="MLSKEDNELLTQINPSTPMGNLIRRYWVPALLSEEIPQADCPPV
                     RVRMLGEELVAFRDSQGRIGLLGERCAHRGTSLFYGRNEECGLRCIYHGWKYDVDGNV
                     LDTPAEPGDSDFRKKLHHTAYPTHEAGGVIYAYLGPRDKMPLFPNYEWTQVPLDQTYV
                     TKCLLECNYLQGLEGECDSSHLSFLHRAFSNERNQLLYKSDTSPVYETEETDFGVRLI
                     ATRNTADNQRYIRFSAFVMPVYGCVPAGRPTNELDGYEIHTYVPADDTHCWRYDIGFR
                     RSQPVRADEVHRRKQINPDYTRIRNARNNYLQDRQMQKTVNFTGIEDFLNHDACATES
                     MGPIFDRSKEHLGVSDKAVIAVRKFLLSSVKDLQNGKELPHLVHHPEHNWFPHVDCFA
                     YLLPTGLTWRQHFDYLTVSARKENPASFASMQEAAS"
     gene            4170..>4607
                     /locus_tag="VFN58_04580"
     CDS             4170..>4607
                     /locus_tag="VFN58_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8917299.1"
                     /translation="MKKIKRSSTKHRSAIKLAEEELRVGAKILEYEMGDIWGHVGVRL
                     PEDEGIAVQMFRRPESGEKDWLVHFDYQLNKLSGVGTPPREAAIYTGIFKARPDVNAA
                     VHSHAPMCVALSLADKAVSCVQMQSDCFGRGVPIYPKPIYILDD"
BASE COUNT         1104 a         1247 c         1228 g         1028 t
ORIGIN      
        1 ttatctcatt cttgccatag cgaactgcag ctttttcagt tcaccttcca gatagagatc
       61 tttcaactga tggcggtcca agtgcggttc gagttgctcc gccggataaa tcttggtacc
      121 acagcggtgg cataccgccg catgataagt tgcgtcctcg tactgaattt caataagttc
      181 taatgggagt gttgtttcca tatgtccctc tctgtctttg atttgcagcg cgagtcaacg
      241 tgcttaagca attgaaatgc caaaatcacc accacccgcg atattgttga aaatcattag
      301 atttttcaat ggcttattcg aggatttcag aagtaaatcc ggtgcttcca gatcaatagg
      361 agtaatagtt tgctaatcaa agtacagttt tgtacaacgt aaacgtattt tcatgtacaa
      421 aacggtcggc cgattcctat ttgtttttct ggggcgtgag ttgtccgtta ggtggggaat
      481 cggtatcgca agccaagcta tagacggaag agtcggaaag ttgacgacgc catagttgtt
      541 gaggtaacaa tccaaataga tcgacattac ggtgaaataa cgcccgggtg ctctgcacgc
      601 cggggaactt gagatggtca cagcgatcga taacacactc cccaccgttg tcttttcgga
      661 agaaaaatcc ggccgattca aagttggcta tgtcgccctc agtaatcccc gcgccctcaa
      721 tgccctagac ttagggatgc tgcgcgtgat ggagggtaag cttcttgaat ggtggcagga
      781 tgacgacctg gcctgcgtgg ttctatacgc tgaatcagag aaggccttct gcgccggcgg
      841 cgatgtaaag tcgctggtta tggcattgca acgagaagga accattgccg cggctaggga
      901 ttatttcacc acggagtact tcgtcgatta tctcgtccac gtctatccca aaccaattct
      961 ttgttgggct gacggcatta ccatgggcgg cggcataggc attatgagcg gggcatcgtc
     1021 tcgtgtggtg actgaacgca cggtgatggc gatgcctgag atcgccatcg gtttatttcc
     1081 cgatgttggt ggcacctatt ttctcaacca gctgcccgcc ggcgtgggtt tgttcctcgg
     1141 tttaaccggt gcgcgcatca gtggcgatga cgcgacttct gtcggcatgg cggatgttat
     1201 cgtgcgctcg gagaaaaggg gagagatctt cgccggacta tcgcgtctca gttggagtcc
     1261 ggatcctcaa aagaacaaac agacactgcg cgactatctt gcttctgtcg ccgaacccga
     1321 tccagccaat agaggtgatc tcctcaaacg actcgacatc gtgaagaaac tgacgctcaa
     1381 agcaaccgtc gaagagatcg acagcggatt tcgaagctgg catggaaacg acgaatggat
     1441 cgagagtggt attcagggtt atctgagcgg ttcgccgact tccgcgaagg cgatctttca
     1501 gcaactgacg agcggaaaag agctgtcgtt gaaacaagct ttcctccgcg aatgggacat
     1561 ggcgctcaac ttctgcgagc gctccgactt ccgcgaaggc gtgagatccc gcttgatcga
     1621 caaagaccaa aagcctcagt ggaacccgcc caccctagcg gggatcaggg acgaggacat
     1681 cgagcgattt ttctccaagc agcacggtca acccgatttg ttggcgcaaa agttgtccga
     1741 gctggcactt ggctaagcag ctcgcttcac ccaatgcata ctcttgacac cgaaggaccg
     1801 tcgcgcaatg gtaatttcgc cagttgagtc tcgaaagcgg ccatgactat aaacgagcta
     1861 ttggaaattg aatttcccgt gatgcgctat gacatcacga tgccgcttct cgaaggacga
     1921 gttgccatcg acggcgtcaa actcaaaccg gttaagtctt cgtcgatgat caataaggaa
     1981 gaccctaaga tgatcgccgg cgagttcggg ctatgcgacc ttaatattgg gtattggcta
     2041 ccggccatcg aagccgggtg ggagatcgtc ggcttaccgg tcttctccaa acgcaaaccc
     2101 gtttatcagc tgatattcac tcatgccgat tccggcatac gctctcccaa agatttggca
     2161 ggcaagaaag taggctcgcg tacgtaccga accgcgctca ccgtctgggc gcgcgggctc
     2221 ctgcaggacc ggtacggtgt tgatttttca aaggtccaat gggttcttca agccaaagaa
     2281 gtctttcccg tccatgaccc agctgcaaag attgaatacg tcgacgacaa gaaaaacatg
     2341 gttgaacggc tgatcggtgg cgagctggat gcgatcatca ctgacatctc cgacaccaag
     2401 ttgtttcaga tcttggagac tcaccccaag gtggagcggc tgtttcccga ctacatcggc
     2461 gaggatcaaa aactctatcg ggagaccggt ctctatactc cggtccacat gatcgtcatg
     2521 agccgaaagc tcgaccggga gcgtcctgat ctcgcggcaa aattttacac tgccttcgaa
     2581 caggcgaagc gaatcgcata tgacgatatt ctcagcgacc gagggggctt ctcggttgtt
     2641 tacttgagag agaaattgaa agagcagatc gcagcctggg acgacccgtg gaaatacggg
     2701 ctcaaagcga ataaatcgac catcgacgcc tttatcaagt acaatataga gcaaggcatg
     2761 gtccgctcgc aattttttta cggtgacctt ttcgcagcag gaacgctcga cacctgatca
     2821 ttcgtcgaga ggatgatttc atgctttcca aagaagacaa cgaactgtta acccagatca
     2881 acccgagcac acccatgggc aacttgattc ggcgctattg ggttcccgca ctcttgtccg
     2941 aagaaattcc ccaagcggac tgcccgcccg tccgcgtgcg gatgctgggc gaagaactgg
     3001 tcgcctttcg cgacagccag ggacgcattg gcttgctcgg tgagcgttgt gcgcaccgcg
     3061 gaacgtcgct cttctacggc cgcaacgaag agtgcggttt gcgctgcatc taccacggct
     3121 ggaaatacga tgtggacggc aatgtgctag atacgccggc ggagcccggc gacagcgact
     3181 tcaggaaaaa actgcatcac accgcctatc ctacgcatga agccggcggc gttatctatg
     3241 cctacctcgg accgcgcgac aagatgccgc tgtttcccaa ctatgaatgg acgcaagtgc
     3301 cgctggatca aacctatgtc accaagtgtc tcctggagtg taattacctc cagggactcg
     3361 aaggcgagtg cgattcctcg cacctttctt ttctccatcg cgcattcagc aacgagcgta
     3421 atcagctgct ttacaagagc gacacatcgc ccgtctacga aacagaagaa acggactttg
     3481 gcgtgcggtt gatcgcgacg cgaaataccg ccgacaacca acgctacatt cgattttccg
     3541 ccttcgtgat gccggtttac ggttgtgttc ctgccggccg gccgacgaat gagctcgatg
     3601 gctacgaaat ccatacctac gtcccggctg acgacaccca ttgctggcgc tacgatatcg
     3661 ggtttcggcg tagccagccg gtccgagcag acgaagtgca tcggcgcaaa cagatcaatc
     3721 cggattacac gcggattcgc aatgcgcgca acaattattt gcaggatcgg cagatgcaga
     3781 agacggtcaa cttcacgggc atcgaggact tcctcaacca tgacgcctgc gccaccgaga
     3841 gcatggggcc gatcttcgac cgcagcaaag aacacctcgg cgtcagcgac aaggcggtca
     3901 tcgccgtgcg caaattcttg ctgagctcag tgaaagacct gcaaaacggc aaagagctgc
     3961 cccacctcgt gcaccatccc gagcacaatt ggtttccgca tgtcgactgt ttcgcgtatc
     4021 tgttaccaac gggcctgact tggcggcagc actttgacta tttgacggtt tcagcaagga
     4081 aggaaaaccc ggctagcttc gcttccatgc aggaggctgc gtcgtgaggc aaatcgttct
     4141 tacatcgcgt cggtgacgcc tgagacacca tgaagaagat aaaacgatca agcaccaaac
     4201 accgatccgc catcaaactc gccgaggaag aacttcgcgt cggcgccaag attctcgaat
     4261 acgaaatggg tgacatatgg ggccatgtcg gcgtgcgcct gcccgaggat gagggcatcg
     4321 ccgttcagat gtttcgtcgt cccgaaagcg gcgagaaaga ttggctcgtt catttcgact
     4381 accaactgaa caagctcagc ggtgttggca cgccgccgcg cgaggcggcg atttacaccg
     4441 ggatcttcaa ggcgcgccca gacgttaacg cagccgtcca ttcccacgca cccatgtgcg
     4501 tcgctctcag cctcgcagac aaagcggttt cctgcgtgca aatgcaatcc gactgcttcg
     4561 gccgcggcgt gccgatctat ccgaaaccga tctacatttt agatgac
//
LOCUS       DASSWZ010000187         4617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1152347, whole genome shotgun sequence.
ACCESSION   DASSWZ010000187 DASSWZ010000000
VERSION     DASSWZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152347"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..996)
                     /locus_tag="VFN58_04585"
     CDS             complement(7..996)
                     /locus_tag="VFN58_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET8917300.1"
                     /translation="MNKTVILQRAKEVLNIEATGIFSLIDRLDDHFIQAIELLYNCRG
                     KVVVTGLGKSGLVCRKIAATFSSMGTPSFFLHAGDANHGDMGMIMEGDVVLAVSNSGE
                     TEEILTLIPTLKRIGVRIVAMTGNHDSTLSRAADVVLDAGVTEEACPLGLSPTASTTA
                     ALALGDALAVILLEKKGFKEEDFALRHPGGILGRKLLLRVEDLMHRDGDVPLVGEETL
                     MKEAILVITSKRLGVTGVTDKRGDLIGVITDGDLRRGLQAHGDGILSKRAEEIMTLKP
                     RSIEKDALASQALARMEENLPRPITSLFVVEKPGDRVPVGILHIHDLLKAGIV"
     gene            complement(1005..1829)
                     /gene="kdsA"
                     /locus_tag="VFN58_04590"
     CDS             complement(1005..1829)
                     /gene="kdsA"
                     /locus_tag="VFN58_04590"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET8917301.1"
                     /translation="MSTKLVKIGNIEIGAGRPLAVIAGPCVIESRESALKHASLLKEA
                     ADRVGVPYIFKSSYDKANRSSVDSYRGPGLDKGLEILAEVKKKTRVPILTDVHDREQV
                     ASVRDVADVLQIPAFLCRQTDFVVAVAQSGRVVNVKKGQFLAPWDIGNIVDKILSANN
                     DQILLTERGASFGYNNLVSDMRSLVVMRELGYPVVFDATHSLQLPGGLGKASGGERKY
                     IGPLARAGVAVGVDALFMEVHENPDQALSDGPNSLPLREFEGVLRIVKEIDGILKR"
     gene            complement(1859..3505)
                     /locus_tag="VFN58_04595"
     CDS             complement(1859..3505)
                     /locus_tag="VFN58_04595"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET8917302.1"
                     /translation="MAGVKTKFIFVTGGVVSSLGKGLASASIGALMESRGLKVTLLKM
                     DPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERYVSTAMGRKNNCTTGQIYETV
                     IAKERRGDYLGGTVQVIPHITDEIKRRILAASEGYDIAIGEVGGTVGDIEGLPFLEAI
                     RQFAWDWGRENVLYVHLTLVPYITGGGELKTKPTQHSVKELTGLGIQPDILLCRTDRF
                     LDRKIKAKIAHFCNVKEESVITAKDVEWIYEVPLVFHEEGLDAQIVEKLHMWTGSPNL
                     QKWQKTIQVLNAPKSAVKIAVVGKYMSLKESYKSLFEALVHGGIANETKVEMECIEAE
                     KIEEVGAPELLSQADGILVPGGFGDRGSEGKIETIRYARENHIPFFGICLGMQMAVVE
                     FARNVCGLVGANSSEFDADTPHAVIHLMESQKSIDAKGGTMRLGAYPCVLQDDSLALK
                     LYGRKKISERHRHRYEFNNGYVEGFSAKGMLLSGVSPDGRLVEIIELKDHPWFLGCQF
                     HPEFKSRPMDCHPLFKGFVKAALQNRPALREVPRMKVART"
     gene            complement(3515..4288)
                     /gene="kdsB"
                     /locus_tag="VFN58_04600"
     CDS             complement(3515..4288)
                     /gene="kdsB"
                     /locus_tag="VFN58_04600"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET8917303.1"
                     /translation="MAVVAIIPARYGSTRLPGKPLARIGGKPMIQHVYENASQAKGLD
                     RVLVATDDRRVEETVRGFGGEVMITSKDHASGTDRLAEVARKIKADWFVNVQGDLPFI
                     QSETITRAVQPLRRDRSIPMGTVGTPIFDEAEWRNPNIVKVLTDCKGFALYFSRSPIP
                     YVRNGAVDLNGNKLAQGSKPRLWGNRHIGLYVYRRDFLLKFARLRPTALEQIESLEQL
                     RALAYGHRIFVVAVDERSVEVDTPADLAKAENYLRQHRN"
BASE COUNT         1064 a         1354 c         1097 g         1102 t
ORIGIN      
        1 ggctgcttaa acgatcccgg ctttgagaag gtcgtgtatg tgtaggatcc ccaccggcac
       61 cctatcaccg ggtttctcca caacaaaaag cgaagtgatc gggcgaggca gattctcttc
      121 catccttgca agagcctgtg acgcgagggc atccttctca atcgatcgtg gctttaaggt
      181 cataatctct tctgctctct tgctgaggat gccatcgccg tgggcttgca gtcccctgcg
      241 taagtcgccg tcggtgatga ccccgatcag atcgccccgt ttatcggtta ctccagtgac
      301 acccaaacgc tttgaggtta tgactagaat ggcctccttc atgagagttt cctcgcctac
      361 cagcggtaca tcgccatccc ggtgcatcaa gtcctcgacc cgcagcagca atttgcgtcc
      421 aagtattcct cccggatggc gcaacgcaaa atcttcttct ttgaaaccct ttttctctaa
      481 gaggataacc gcaagggcgt cccccagagc gagcgccgcg gtggtacttg ccgtcggcga
      541 tagcccgagg gggcaagcct cttccgtgac ccccgcatcg agaacgacgt ccgctgcacg
      601 ggagagggtt gagtcatgat tgccggtcat ggcgacgatc ctcacaccga tacgcttgag
      661 agtgggaatc aacgttagaa tctcttcggt ctcgccgctg ttggagacgg ccagaaccac
      721 gtcgccttcc ataatcatcc ccatatcgcc gtgattagca tccccggcgt gaaggaaaaa
      781 ggaaggtgtg cccatgctcg aaaacgtagc ggctattttt cggcaaacga gcccggattt
      841 ccccagcccg gtgacgacca ctttgccgcg acagttgtaa agtaattcaa tcgcttggat
      901 aaagtgatcg tcgaggcgat cgatcagcga aaagatgccc gtcgcttcga tgttcaacac
      961 ctctttcgct cgctgcaaga tgaccgtttt gttcatgtcc cggattaccg tttcaaaatg
     1021 ccatcaattt ccttaactat ccgtagcact ccttcaaact cccgcaatgg cagtgaattg
     1081 ggaccatcgc ttagcgcttg atccggattt tcgtgaactt ccatgaacag cgcatcgacg
     1141 cccaccgcga caccggcgcg agccaacggg ccgatgtatt ttctttcgcc gccggatgct
     1201 tttcctaacc cgcctgggag ctgcaagcta tgggttgcat cgaaaaccac aggatacccc
     1261 agttctcgca tcaccactaa cgacctcatg tccgaaacca gattgttgta gccgaaggaa
     1321 gcgccccgtt ccgtgagtaa gatttgatcg ttgttggcgg agagaatttt atcgacgata
     1381 ttgccgatat cccacggcgc cagaaactgc cctttcttta cattgacgac tctccctgat
     1441 tgagcgaccg cgacaacgaa gtctgtctga cggcagagga acgccgggat ttgcagcaca
     1501 tcggcgacgt ctctcacaga cgcaacctgc tctctgtcat ggacgtcggt gagtatgggt
     1561 accctggtct ttttcttgac ctcggcgagg atttccaaac ccttgtccaa tcccggcccg
     1621 cgataggaat cgaccgatga acgattcgcc ttgtcgtaag aagatttgaa gatgtaggga
     1681 accccaaccc gatcagcggc ttctttaagc aacgaggcgt gttttagcgc cgactccctg
     1741 ctttcgatca cgcagggacc agcgataacc gcaagcgggc gcccggcgcc gatctcgata
     1801 tttcctattt ttacgagctt cgtgctcatg gataaaaaga cccgacaact cgcaacgatc
     1861 aggtgcgcgc gactttcata cggggtactt ctctgagggc tggccgattt tgcaaggccg
     1921 ctttgacgaa gcctttaaag agaggatgac aatccatcgg acgcgatttg aattccgggt
     1981 gaaattggca gcccaggaac cagggatgat ccttaagttc gatgatctcc accagacgtc
     2041 catccggtga cacaccgctc aatagcattc ctttggcgct gaagccttca acgtagccgt
     2101 tgttgaattc ataacgatgc cgatgccgct cgctaatctt cttccgaccg tagagcttca
     2161 aggccagcga gtcatcctgg agcacgcagg ggtaagctcc caacctcatg gttccgccct
     2221 tggcatcaat actcttttgt gactccatca ggtgtataac cgcatgaggg gtgtccgcat
     2281 cgaactcact ggaattggcc ccgacgaggc cgcaaacatt gcgcgcaaac tcaaccaccg
     2341 ccatctgcat tcccaaacat atgccgaaga acgggatatg attctctctt gcatagcgaa
     2401 tggtctctat cttaccttcg ctaccgcgat cgccaaatcc gcccggcacc aagattccgt
     2461 cggcctgact taaaagttca ggtgctccca cttcttcgat tttttcagcc tcaatacact
     2521 ccatctcgac tttcgtctcg ttggcgatac cgccgtgaac cagagcctcg aaaaggctct
     2581 tgtatgactc tttgaggctc atgtatttgc caacaaccgc gatcttgacc gcggatttcg
     2641 gtgcgttcag gacttgaata gtcttttgcc acttctgaag attcggcgaa cccgtccaca
     2701 tgtgaagttt ctcgacgatc tgcgcatcga gcccctcctc atgaaagacc aacggaactt
     2761 cgtagatcca ctccacgtcc ttagcggtaa taaccgactc ttcttttacg ttacaaaaat
     2821 gagcgatttt cgctttgatt tttctgtcaa ggaagcgatc cgttcgacat aaaagaatat
     2881 cgggttggat tcccaggccc gtcagctcct ttacgctgtg ttgagtcggc ttggtcttaa
     2941 gctcccctcc gccagtaata taaggcacca aggtcaggtg aacatacagc acattctctc
     3001 ggccccaatc ccaggcgaac tgccggatcg cttccaggaa aggcagtcct tcgatgtcac
     3061 cgaccgttcc gccgacctcg ccaatcgcaa tatcgtaacc ttcggatgca gctagaattc
     3121 gccgttttat ttcgtccgtt atgtgcggaa tcacctgcac ggtcccgccg aggtaatcgc
     3181 cccttcgctc cttggcgatg acggtctcgt aaatttggcc ggtcgtgcaa ttatttttcc
     3241 ggcccatggc ggttgagaca tagcgctcgt agtgtcccaa atccaaatcc gtctcggcgc
     3301 cgtcatccgt gacgaaaact tcaccatgct ggaacgggct catcgttccc ggatcaacat
     3361 tgatgtaagg gtccatcttc aacaaagtga ccttcaaccc acgactctcc atcaacgccc
     3421 cgattgacgc cgaagccagt cccttgccta acgaagagac gactccaccg gtcacaaaga
     3481 taaatttcgt ttttactcca gccatggctc tccttcagtt cctatgttgt cttagatagt
     3541 tttccgcttt cgccaaatct gccggcgtat cgacctccac ggaccgctca tccacggcta
     3601 cgacaaatat ccgatgaccg tacgccagcg cccggagctg ctcgaggctt tcgatttgtt
     3661 ccaacgctgt gggacgcaag cgggcgaatt tcagcaagaa atctcgccta tacacgtaaa
     3721 gtcctatatg acgattgccc cataatcgtg gtttagaacc ttgcgccagt ttattaccat
     3781 tgagatcaac ggcaccattc cgcacataag gaatcggcga acgggaaaaa tacagcgcaa
     3841 agcctttaca gtctgtaaga accttaacga tattcgggtt gcgccactcg gcttcatcaa
     3901 agatcggcgt gccgactgtc cccatcggaa tcgagcggtc acggcgcagg ggctgaaccg
     3961 cgcgcgtgat cgtctccgac tgaataaacg gcagatcccc ttgtacattt acgaaccaat
     4021 ccgctttaat ctttcgcgcc acctcggcca aacggtccgt gccgctggcg tggtccttcg
     4081 acgtgatcat cacctcaccg ccaaaacctc ggactgtttc ttccacccgc cggtcgtctg
     4141 ttgcgaccaa aacccgatcc aaacctttgg cttgtgacgc attttcgtaa acgtgttgga
     4201 tcattggttt gccgccgatc ctcgccaaag gttttcccgg aagacgggtc gaaccataac
     4261 gcgccggaat aattgccaca acggccatcg attcactgtt ctagtggtgc gctgaagaat
     4321 gcaagagagg tcaaagttgc gtttcgttcc caaaaataaa aaccgcaatg gccctctcta
     4381 agaaagcgca tcacggactt caaatatacg ccgcagccga aaaggtgtca atgacgaatc
     4441 caagaagggg cgacgaatcc gagaaatttc tccagtgcta aggtttttcg ccggctcgag
     4501 ataaacatcg tccatacaac ttgacttgtt attcccgccc tatgatagga atgacgttaa
     4561 gaatcactaa ggatatgtat ggccgacacc attaagctca ccgcgggatt cgctacc
//
LOCUS       DASSWZ010000188         5337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1180962, whole genome shotgun sequence.
ACCESSION   DASSWZ010000188 DASSWZ010000000
VERSION     DASSWZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180962"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFN58_04605"
     CDS             complement(<1..255)
                     /locus_tag="VFN58_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917304.1"
                     /translation="MDTQPMEILLVEDNEDDILLEQEALADAKLVNLMCVVRDGEEAM
                     AYLRRQGKYQNVKMPGLILLDINMPKKNGFEVLNEIKSDPA"
     gene            complement(255..2177)
                     /locus_tag="VFN58_04610"
     CDS             complement(255..2177)
                     /locus_tag="VFN58_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8917305.1"
                     /translation="MSQLDTPSNFERHQLRADDRAGGSSELQTLQEISRTILDSLDLK
                     LMMEGILNKASQIGGFDIGMICFVTTARQTLEPVAHCGFRNHENLTTYREHMREQSTA
                     GIVDRVIATKEIRVVDLQKTEGIRTFRSEAVRSLVIVPLRANQDALGIMYLGSRRGRE
                     FEPGQIRLLEAIGTQAGIAVQKVRMFEAVERRAKEQETLNVIATATSQSLELKELFEI
                     AADKTVMVTERQRINFRLKDPLTGKVNVVAYRGFTEDEIETLRRITPHPMSEQVFASG
                     KPLVINDHVGPGVSGLLARTQSVAWIPIKAGPKVVGVLGISDDQSRPFASNEVELLQA
                     IGSVIGVAIENARLFEQTRKQAAELEQSNKAKDELLVVMARQKEELSRLNAGLECEIA
                     ERGRARAEIAAKNRDLETLLYVTSHDLREPLRAIENFSRIVNDRYSERLDDKGQDFLR
                     RIISGAQRLNRLLDDILTLSRSQRIGAASEEVDGERIVTEALKRLEGKINATHARIHV
                     AKDFQGLRVDKTWATQAVYNLIANALKFTREGEAPDVEIVPYRPQAPGRNAIGIAVRD
                     RGPGIAPEHAERIFQLFQRAVGREIEGTGAGLAIVRQIAERHGGSAWVEPRQGGGSEF
                     IITFAQGKDLAGGTQQ"
     gene            complement(2253..4820)
                     /locus_tag="VFN58_04615"
     CDS             complement(2253..4820)
                     /locus_tag="VFN58_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8917306.1"
                     /translation="MRLILLVFIAVVPALILILHNAKRDRDVSAERIQEDAQRIVEIA
                     ASRQTRFIDSARQLLAVLAELPEVAWGDSAGCNRFVKGLTDRYSVYGNLGRIDAEGNL
                     VCSAIGFSGTLNLADRSYFRRAKDSKDFAIGDYQIGKVTNRGSVDFGYPILNRSGEVE
                     GVIYAALDLTWLTHLAAEARLPEGASLSILDSQGTILARFPDPERWAGKPVPDAPLFQ
                     ILQLRNQATRELIGVDGVKRLYAFTTLSGKPQTGQMYVVVGIPKEIAFAEVDQRLQRN
                     LGWLGVACLLALATAWFIGNRHVVAYVTEHARAEEARLRLASIVESSEDAIIGKSLDG
                     TITSWNDGAEVMYGYTEEEVKGQPITILNPPDRANEIPQLLEIVQQGKGINRYETERI
                     RKDGRRLYVSASVSPIRDHEGKVVGASTIGRDITALRKVEEKLRAHASQMETLYTIGQ
                     EMGRTLALDEVIQLALDRVVSASGFDVAFIHFAGKSSAPAVYAAGCASRPSAEQTQLL
                     ARLGGDLKRQIAVCKQPWFIEDVAVRPELKELREVGGMRAMAVLPLCGGEQRRGTLTL
                     MSPRVHTFGPEESQFLQALGQQIALAIENAQLYGATVEVNAHLQDEIEERNRAEKTLA
                     DFTAMVVHDLRSPLSNVVSIAESLQNGLFGSVNEQQNKWLWKIENNCKSLIEHVSDFL
                     DLSKIEAGHIELVKKAVDLELLIHEILVEHSIQADKRRISLRSQIDRGLPTTWVDPRR
                     FNQVLSNLLSNALKFSDDGGDIEVGASRGNGNEVKAWVKDAGIGIASDEIGQIFEKYR
                     QISSGRSSGQKGTGLGLVICKKIVEAHGGRIWAESQEGRGATVFFSLPVDALRSGPN"
     gene            complement(4956..5252)
                     /locus_tag="VFN58_04620"
     CDS             complement(4956..5252)
                     /locus_tag="VFN58_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917307.1"
                     /translation="MKIGVFSGLRSRLVCLIARRLLKKRIFPVRMNGLGRLLLSRRGS
                     GGPEIKDPKSKKVEKDLHRHLAWIQILCEVDRKFLASLDLRLMTDALFTVALPG"
BASE COUNT         1102 a         1595 c         1332 g         1308 t
ORIGIN      
        1 agcagggtcg gattttattt cattgagcac ctcgaagccg tttttcttcg gcatattgat
       61 gtctaggaga atgaggcccg gcattttgac gttttggtac ttgccctggc gccgcaagta
      121 ggccatggct tcttcgccat cccgcacgac acacatcaag ttgacgagct tggcatcggc
      181 taacgcctcc tgctcgagca aaatatcgtc ttcattatct tccaccaaga ggatttccat
      241 gggttgagtg tccattattg ttgagtgcct cctgccaaat cttttccttg ggcgaacgta
      301 atgataaatt ccgagccgcc gccttgacgg ggctctaccc aagcgctgcc gccgtgccgc
      361 tcggcaatct gtcgcacgat ggctagcccc gctcccgtcc cctcgatctc tcgccctaca
      421 gctcgctgga atagttgaaa tatgcgctcc gcgtgctccg gcgcaatccc cggaccacga
      481 tcgcgcactg cgataccgat cgcattccgg cccggcgctt gcggccgata gggcacgatc
      541 tccacgtcag gcgcctcgcc ttcgcgggta aacttaagag cattggcgat caggttatag
      601 accgcttggg tcgcccaagt cttgtccact cgcagtccct ggaagtcttt cgccacgtga
      661 atccgagcat gggtcgcgtt gatcttacct tcgagacgct ttaaggcctc ggtcacgatc
      721 ctttcgccgt caacttcttc actggccgcc ccgatccgtt gggagcggga gagtgtcaag
      781 atgtcatcga gaagacggtt caaccgctgg gctcctgaaa tgatgcgccg taagaaatcc
      841 tggcctttat cgtcaagccg ctcgctgtag cggtcattga cgatccgcga gaagttctcg
      901 atggcgcgca gcggctctct gagatcgtgg gacgtcacat aaagtaaggt ctcgaggtcg
      961 cggttcttgg cggcgatttc cgccctggct cgtcctctct cggcgatttc gcactccaat
     1021 cccgcgttga ggcgggataa ttcttctttc tgtcgcgcca tgacaaccaa caattcatcc
     1081 ttcgctttat ttgactgctc cagctcagcc gcctgttttc ttgtttgctc aaacagcctg
     1141 gcattttcga tggcgacacc gatcacgctc ccgatcgcct ggagcagctc cacttcattg
     1201 gaggcaaagg gtcttgattg atcatcggaa atgcctagaa ctccaacgac ctttggcccg
     1261 gctttgatgg gaatccacgc aaccgactga gtccttgcca acaaccctga aactccgggc
     1321 cctacgtgat cattgatgac cagtggcttt cccgatgcga aaacctgttc gctcattggg
     1381 tgtggggtga tacgacggag tgtttctatc tcgtcctcgg taaatcctcg ataagctacg
     1441 acgttaacct tgcccgtaag cggatctttg agtctgaagt taatccgctg tcgttccgtt
     1501 accatgacgg ttttatccgc ggcgatctca aaaagctcct tgagctcgag cgactggcta
     1561 gtagcggtgg cgataacatt caacgtctct tgttctttgg ctcgccgctc aaccgcctcg
     1621 aacattctga ccttttgaac ggcaatcccc gcttgggttc caatggcttc aaggaggcga
     1681 atctggccgg gttcaaactc tctgccccgg cggctgccga ggtacatgat gcccaacgcg
     1741 tcctgattcg cccgcagcgg gacgatgacc aaagagcgca ccgcttcgct cctaaaagta
     1801 cgtatgccct cggtcttttg gagatcgact acacggatct ccttcgttgc gatcacccga
     1861 tcaacgatgc cagcggttga ttgctctctc atatgttcgc gatacgtcgt caaattctcg
     1921 tgattgcgaa atccacagtg ggcgacgggt tccagcgtct gtcgcgctgt cgtcacaaag
     1981 caaatcatcc cgatgtcgaa accaccgatt tgggaggctt tattcagaat cccctccatc
     2041 atcagcttga gatcgagcga atccagaatc gtacggctga tctcttgcag tgtctgaagc
     2101 tcgctagaac cgcccgctcg atcatctgcg cgaagctgat ggcgctcaaa attgcttgga
     2161 gtgtcgagct gactcatagt ggctccgatt ccgaagataa aaccctttga agctccgtac
     2221 cgtcgaccct tcgttctgca acgcggttaa ttttagttcg gcccagatct caaggcgtct
     2281 actggcaagg aaaagaagac cgttgcgcct cttccctctt ggctttcagc ccagatccgt
     2341 ccgccatggg cttcgacgat ctttttacag atcaccaatc ccaggccggt tcctttttgc
     2401 ccggagcttc ttccgcttga aatctgccgg tacttctcaa atatctgccc gatttcatcg
     2461 ctggcgatgc cgatccccgc gtctttgacc cacgccttga cctcgttacc gttaccgcgg
     2521 ctagcgccca cttcaatatc gcctccgtcg tcggagaatt tcagcgcgtt gctgagaaga
     2581 ttgcttaaca cctggttgaa gcggcgggga tccacccagg tcgtcggcag gcctctgtcg
     2641 atctgactcc tgagtgaaat ccgtctcttg tcggcctgga tcgagtgctc caccaagatt
     2701 tcgtggatca gtagctcgag atcaacggct ttcttgacca gctcgatatg cccggcttcg
     2761 atttttgaaa gatccaagaa atcgctgaca tgttcgatca ggcttttgca gttgttctcg
     2821 atcttccaca gccacttgtt ctgctgctca ttgaccgacc caaacaatcc attttggagc
     2881 gactctgcaa tcgagaccac atttgacaga ggtgatcgca gatcatgaac caccatggcc
     2941 gtaaaatctg ccaaggtttt ttctgcccgg ttgcgctctt ctatctcgtc ctggagatga
     3001 gcattgactt ctactgtggc gccatagagt tgggcatttt cgatggccag agcaatctgt
     3061 tgccccaggg cttggagaaa ctgtgactct tcaggaccaa aggtatgcac tctcgggctc
     3121 atcaatgtga gcgtgccccg gcgctgctca cccccgcaca acggcagcac tgccattgcc
     3181 ctcattcctc ccacctcccg cagctctttt agctccggcc gaaccgcgac atcttcgatg
     3241 aaccacggct gcttgcacac agcgatctgt cgtttcaaat cgcctcccag tcgcgccagc
     3301 agctgcgttt gttccgctga cggtcgcgac gcgcaaccgg cagcgtaaac cgcgggagcg
     3361 gatgatttgc ccgcaaagtg tatgaatgcg acgtcaaaac cggatgccga aacgactcga
     3421 tctaaagcca attggatcac ctcatcgagg gcgagcgttc tgcccatttc ttgtccaatg
     3481 gtgtagagcg tttccatctg actcgcatgg gcacgaagct tctcctcaac tttgcgcagt
     3541 gcagtgatat cgcgaccgat ggtcgaagca ccaacgactt ttccttcatg gtcccggatc
     3601 ggagatacgg aagcggaaac gtataaccgg cgaccgtctt ttctgatccg ttccgtttca
     3661 taacgattga ttccctttcc ttgctggacg atttccagca actgaggaat ttcattggct
     3721 cggtccggcg ggttcaatat tgtaatcggc tgtcccttga cttcctcctc cgtgtagccg
     3781 tacatcacct ccgcgccgtc gttccagctg gtaatggtgc catctaagga cttgccaatg
     3841 attgcatctt cggaagactc gacaatcgaa gccaatcgga ggcgggcttc ttcggccctt
     3901 gcgtgctcgg taacataagc gaccacgtgc cggttaccga taaaccaggc cgttgcgagc
     3961 gccaataagc aggccactcc tagccagccg agattccgct gcagtctctg atcgacctcg
     4021 gcaaaagcaa tctccttcgg gataccaacg accacataca tctggccggt ctgcggcttg
     4081 ccgcttagag tcgtgaacgc gtacagccgt ttcacgccat ctacgccgat gagttccctt
     4141 gttgcttggt ttctcagctg gagaatctga aacaggggtg catccggcac aggcttgccg
     4201 gcccacctct ccgggtccgg aaatctagct agaatagttc cctgactgtc caaaatggat
     4261 agtgatgccc cctcgggcaa tcgagcctcc gcagccaaat gggtcagcca agtcaagtct
     4321 aacgccgcat agatcacccc ctccacctcc ccggatcgat tcagtatcgg atagccaaaa
     4381 tcaacgcttc ctctgttggt gactttgccg atctggtagt ccccgatggc aaagtccttg
     4441 gagtccttcg ccctgcggaa ataggaacga tcagcgaggt taagagtgcc ggagaatccg
     4501 atggcactgc agaccagatt gccctcggca tcaatgcgac cgagattccc gtaaacgctg
     4561 tagcgatccg tgagtccttt cacaaaccga ttgcagcccg cggagtcacc ccaggccact
     4621 tcaggcaatt cggccagtac ggctaacaat tggcgcgccg aatcgatgaa tcgcgtttgt
     4681 cgactggcgg caatctcaac gatccgctga gcatcttctt gaattctctc ggcgctcaca
     4741 tcgcgatcac gcttggcgtt atgcaggatg aggatgagcg ccgggacgac ggcaataaac
     4801 accagcagaa tgaggcgcac acgaagtccg tacctaggag atgaactcag aggggatttt
     4861 tctttgtgca ggtttttcac ccttggagtc cgctgcccgc tattgaaagc tcattctagg
     4921 atttgccggt tgctaaaaag actgcttcca gcctgctagc caggcagagc aaccgtaaac
     4981 aacgcgtccg tcatcagccg gagatcgagg ctggcgagga attttcggtc cacttcgcaa
     5041 aggatctgga tccacgcaag atggcgatgc aaatcttttt ctactttctt gcttttggga
     5101 tctttgattt ctgggccacc ggacccccgt ctcgaaagca gtagccgccc gagcccattc
     5161 atcctcaccg ggaaaattct ctttttcagt agacgcctgg caatgaggca aaccagtcgc
     5221 gagcgaagac ccgagaacac gccaatcttc atatttcacg actctctttt tcaagtttga
     5281 caaccgcagt ctccctattt tccacccact gcctcagctg tgtttcacgc aggcgcc
//
LOCUS       DASSWZ010000189         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1265877, whole genome shotgun sequence.
ACCESSION   DASSWZ010000189 DASSWZ010000000
VERSION     DASSWZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265877"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFN58_04625"
     CDS             <1..625
                     /locus_tag="VFN58_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917308.1"
                     /translation="GRFRGVARIDDKTPKSELQRLHDGGVRGVRFNLLDRPRGNVKLD
                     VLDRCVEHIVEFGWSVDLHIDMKNLLEQENRIRRMPIPVVIDHIARVKPAEGLGQPAF
                     QLLLDLLKAKHVWVKVSGADKICNTKVHSYFGLPFVEVIPFARAVIAAAPDRTIWGTD
                     WPHSNNFASGQTPNDGDLVDLLAAFAPDEQIRKKILVENPAALYGFK"
     gene            724..1437
                     /locus_tag="VFN58_04630"
     CDS             724..1437
                     /locus_tag="VFN58_04630"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET8917309.1"
                     /translation="MKRYNINRGKQIFFIVGIAALCAAAATSVSAATLKGNVRYAGAR
                     VEKKTVPVTIDQYLCGKEKEAGDLVLSSTSGIRNVVVSLHGVPAGSKAPANGAPAKMD
                     QKQCVFVPRVVVVPVGGTVEFLNSDRLLHNVRGSGKENPPFNRAQPHARTISIVFKSP
                     EILRVDCDLHSWMRGWIVVAEHPFYAVTNEEGEFVFENVPPGKYKLQAWQETLGRANQ
                     EVTVAGEGTQTITVRMEKK"
     gene            complement(1452..1898)
                     /locus_tag="VFN58_04635"
     CDS             complement(1452..1898)
                     /locus_tag="VFN58_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HET8917310.1"
                     /translation="MSRRHKQWLVGAIVVLGFALGQLIGGGLTPPVWPGQAEQFTPEF
                     KEVRKSFDAKHTPKIVAPDTVKRGEWFDVTVSIGEHPSLSEHFVRYIALYINTAEISR
                     VYLHPVYSFPKVTFTIALDEGGSLRVLEEPTHSAAWEASKKIKVIP"
     gene            complement(1936..2997)
                     /locus_tag="VFN58_04640"
     CDS             complement(1936..2997)
                     /locus_tag="VFN58_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET8917311.1"
                     /translation="MASRPSRILAVDDNKQNLDLLTKALTSAKYEVITANDGLTALNL
                     VENASADLVLLDVMMPGMSGYEVCEKIRANAATRLLPVVMLTALHDVSHRIRGIESGA
                     DDFLTKPFNREELLTRVKSLLRIKTLHDDIETRNHLLRTLFGRYLSEEVAAEIIADPE
                     RQLKLGGEKREVTILFGDLRGFTPLAERLDPQDAVDILNVYLTHVIDIVFEYRGTLDK
                     FRGDGFMAFFGAPIGHEDHPSSAVRCALTLQERLKGVSFTKFPDLHLHMGIGINTGIV
                     IAGNIGSERRTDYTVIGNEVNIAQRFESNAGPGQILITGSTFERVKGAVEVRDLGLLR
                     VAGKQEGVMAYDVLRWSES"
     gene            complement(3025..3399)
                     /locus_tag="VFN58_04645"
     CDS             complement(3025..3399)
                     /locus_tag="VFN58_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917312.1"
                     /translation="MSDKTVLYVEDNEYNLKIVRQLLGRTSYRLIEAVDGQLGVETAL
                     RELPDLILMDIQLPKLSGLDATRRLRADPKTAAIPIIVITSFALSGDDLKAKDAGASA
                     YLAKPYSPRELLQMIRKFVPEN"
     gene            complement(3396..>4490)
                     /locus_tag="VFN58_04650"
     CDS             complement(3396..>4490)
                     /locus_tag="VFN58_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8917313.1"
                     /translation="PLYEQIAGLVDQIVLAEQSKMTGLRANVAAETRRTQILMGVFVV
                     SAIFLALLLGFVISWSFILPVRKAHGFLTEVAKGNFQATINVPNRDEFGDLAERMNQM
                     TLELHNLYEDQRAAAQELQILNDRLTEASKAKSEFLANMSHELRTPMNAILGFVEMLL
                     DDIYGEVPPHLREPLVDVQVNGKHLLNLINDVLDLSKIEAGRMELVLGEYSVQDVVET
                     VRTSLQSLALEKGLGFEAVAEDDIPLAFGDGRRITQCLMNLVGNALKFCKRGRVAVGV
                     ERQGDDLLYHVADTGIGIPKEELKNIFTEFKQVDTAMTREFSGTGLGLNITKKFVEMH
                     GGRIWAESELGKGSTFFFSIPLRVNDGGTL"
BASE COUNT         1018 a         1274 c         1190 g         1008 t
ORIGIN      
        1 cgggcgcttt cggggcgtcg cgcgtatcga cgacaaaacg ccgaagagcg aattacaaag
       61 attgcatgac ggcggcgtgc gcggcgtccg tttcaacttg ctcgaccggc cgcgcggcaa
      121 cgtaaagctc gatgtcctgg atcgctgcgt ggagcacatc gtggaattcg gttggtcggt
      181 ggatctgcac atcgacatga aaaatttgct ggaacaggaa aaccgcatcc ggcgaatgcc
      241 gattcccgtg gttattgacc atatcgcacg ggtgaagcct gccgaaggtc taggccagcc
      301 ggcttttcaa ctgctcttgg atctgttgaa ggcaaagcac gtctgggtga aagtgagcgg
      361 cgcggacaag atctgcaata ccaaggtgca ttcctacttc gggctgccat ttgttgaagt
      421 gattcccttc gctcgtgcgg ttatcgccgc cgcccctgac cgaaccatct ggggcaccga
      481 ctggccccac tccaataatt ttgcttccgg ccaaacgcct aatgatggcg atttagtcga
      541 tttgctcgcc gcctttgcgc cggacgaaca aattagaaaa aagatcctgg tggaaaatcc
      601 tgcggcgctt tatggattca aataattcgt caaaggagta tcaaaaaggg gcgccaagtg
      661 tgttaaatat gattagataa ggtcgtccgg tatcagtcgt tttttttcaa aggacacaaa
      721 tcgatgaaac gctacaacat caatcgaggc aaacaaatct ttttcattgt tggtatagcg
      781 gcgctctgtg ccgccgctgc cacaagcgta tcagcagcaa cactcaaagg gaacgtgcgg
      841 tacgcagggg ctcgagtaga aaagaagacg gtgcctgtga cgattgatca atatctgtgt
      901 ggcaaagaaa aagaagctgg agacttggtg ctctcctcta ccagtggaat ccgcaatgtc
      961 gtcgtatccc tccacggtgt ccccgccggc tcgaaagcac cggcgaatgg cgcgcctgcg
     1021 aagatggatc agaaacaatg cgtattcgta ccccgcgtgg tcgtcgtacc ggtcggcgga
     1081 actgtggaat tcctcaatag tgaccgccta ttacataatg tgaggggcag cggcaaggag
     1141 aatccccctt ttaaccgcgc gcaaccccac gcgcgaacca tttccatcgt attcaaaagt
     1201 cctgagattc ttcgcgtgga ttgcgatctg cactcctgga tgcgtggttg gatagtggtc
     1261 gccgaacacc ctttctacgc tgtgaccaac gaagagggtg agttcgtctt tgaaaacgta
     1321 ccgccgggca agtacaagct tcaggcctgg caggaaacct tgggccgcgc caaccaggaa
     1381 gtcactgtgg ccggcgaagg cactcagacc atcaccgtgc gcatggagaa gaagtgagct
     1441 tgcccggtca gctaaggaat caccttaatt ttttttgacg cttcccaggc cgcagaatga
     1501 gtgggctcct ccagtacgcg gagcgatcct ccttcgtcca gtgcaatcgt gaaagtgacc
     1561 ttaggaaacg aatagaccgg gtgaagataa actcgggaga tctctgcggt attgatgtac
     1621 agagcgatgt accggacgaa gtgctcgctg agcgacgggt gctcgccgat agagaccgtc
     1681 acatcaaacc attcaccccg cttgacagta tccggagcaa cgattttcgg cgtgtgcttg
     1741 gcatcgaaag atttgcgcac ctccttgaac tcgggggtaa actgctcggc ctgtcccggc
     1801 cagaccggcg gcgtcaaccc accgccgatg agctgaccca gagcgaaacc gagcaccacg
     1861 attgccccta cgagccactg tttatgcctt ctactcattt ggattttcct cccgttatcg
     1921 atcatgttgc gctttttatg actcgctcca gcggagaacg tcgtaagcca taaccccctc
     1981 ttgcttccct gcgactctca gcagccctag gtcgcgaacc tctacagccc cttttacgcg
     2041 ctcaaacgta ctgccggtaa tgaggatttg tccgggtccc gcgttcgact cgaagcgctg
     2101 ggcgatgttc acctcgttgc cgatcaccgt gtaatccgtc ctccgttccg atccgatatt
     2161 cccggcgata acaattccgg tgttgattcc gattcccatg tggagatgaa ggtcaggaaa
     2221 tttggtgaag ctgacaccct ttaagcgctc ctgcagggta agcgcacaac gcacggcgct
     2281 tgagggatga tcttcgtgac ctatgggcgc tccgaaaaac gccatgaatc cgtccccgcg
     2341 aaatttgtcg agagtgcctc tatattcaaa aacgatgtcg atcacgtgcg tcagataaac
     2401 gttgaggatg tcaacggcgt cctgcggatc aagccgctcg gccagcggag tgaacccacg
     2461 caaatcgccg aagagaatgg tgacctcgcg cttttccccg cccagcttta actggcgttc
     2521 cggatcggca atgatctccg cagcaacttc ttcggacaag taccgaccga aaagcgtgcg
     2581 cagcaggtgg tttcttgtct caatgtcatc gtgcagcgtc ttgatcctga gaagggactt
     2641 gacgcgggtc agcagctcct cccggttaaa gggtttggtc aaaaaatcgt cggcgcctga
     2701 ttcgatgccg cggatccgat gggaaacatc gtgcagggcc gtcaacatca ccaccggcag
     2761 aaggcgagtg gcagcgttgg cgcgaatctt ctcgcagacc tcataccctg acataccggg
     2821 catcatgacg tccaagagaa ccaaatccgc ggaagcgttc tccacaagat tcagggctgt
     2881 cagcccatca ttggcggtga tgacttcgta tttcgctgac gtcaatgctt tcgtcagaag
     2941 gtcgagattc tgcttgttgt cgtcgactgc cagtatgcga gaaggtcgac tcgccatatc
     3001 tctatctttc ttttcctcga agcctcaatt ctcaggcaca aacttccgaa tcatctgaag
     3061 cagctcgcga ggactatagg gcttagccag gtaggcagag gccccagcat ccttcgcctt
     3121 caggtcgtcg ccgctgagcg cgaacgaggt gatcacaata atcggaatgg cggcggtctt
     3181 gggatcagcg cgaagcctgc gggtcgcgtc tagccccgat agcttaggga gctggatatc
     3241 cataagaatc aggtcaggca actcgcggag cgccgtctcc acgccaagct ggccatccac
     3301 cgcctcgatc aaccgataag aagtgcggcc cagcaattgg cggacaatct ttagattgta
     3361 ctcgttgtcc tccacgtaga ggacggtctt gtcgctcata gggtccctcc atcgttgacg
     3421 cgcaaaggga ttgaaaagaa aaaggtcgat ccctttccca gctcgctctc cgcccagatc
     3481 cggccaccgt gcatttccac gaacttcttg gtgatgttga gcccgaggcc ggtcccgctg
     3541 aactcccgcg tcatcgccgt atccacttgc ttgaactcgg taaaaatgtt tttcagctcc
     3601 tctttgggta taccgattcc ggtatccgcg acgtgataca gcagatcgtc tccctgtcgc
     3661 tccaccccga ccgccacgcg gccacgtttg cagaatttca gcgcgtttcc caccaggttc
     3721 atgaggcatt gggtgatgcg ccttccgtcc ccgaaggcga ggggtatatc gtcctcggcc
     3781 acggcctcga accccaatcc cttttccaag gccagcgatt gaagcgatgt gcggacggtc
     3841 tccaccacgt cctgcacgga atactcacct aaaactagct ccatgcgccc ggcttcaatc
     3901 ttggaaagat ccagaacatc atttatcaga ttgagtaaat gcttgccatt aacttggaca
     3961 tctacaagcg gctctctcaa atgaggcggc acctctccgt agatatcgtc cagcagcatc
     4021 tcaacaaatc ctaggatagc gttcatcggt gtgcgcaact catggctcat gttagcgaga
     4081 aattcggact tcgccttgct cgcctctgtg aggcgatcat tcagtatctg cagttcttgg
     4141 gccgctgccc gctggtcttc atataagttg tgcaactcca gggtcatctg gttcatccgc
     4201 tctgccaggt cgccgaattc atcccggttg ggcacgttaa tggtagcttg gaaattgccc
     4261 tttgcgacct cggtgaggaa cccatgcgct ttccggacag gtaaaatgaa cgaccacgag
     4321 atcacaaaac cgagcagcaa agccaggaat atggcgctta ccacaaagac acccataagg
     4381 atctgcgttc ttcgggtctc tgccgcgacg ttagctctca ggccggtcat cttcgactgc
     4441 tcagcgagga cgatctgatc aaccagcccc gcgatctgtt cgtaaagagg
//
LOCUS       DASSWZ010000190         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_770519, whole genome shotgun sequence.
ACCESSION   DASSWZ010000190 DASSWZ010000000
VERSION     DASSWZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770519"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFN58_04655"
     CDS             complement(<1..227)
                     /locus_tag="VFN58_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917314.1"
                     /translation="MRIKSSISLLIAAIIAGFLDVDAPTALAAQAKPLIKIVAGYGST
                     DGAIAPLWFAKESKLFEKRGLDVRLVGMGTGL"
     gene            complement(241..972)
                     /locus_tag="VFN58_04660"
     CDS             complement(241..972)
                     /locus_tag="VFN58_04660"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917315.1"
                     /translation="MYGWRARIGNISPTTCAEILPYEFYRVAPEGVTFVTTNLVIRDA
                     RESSQVEASWRRFYTAFEDLLHTRVDHITLSGAPLVLANGVDRHRELLQEFRSRFPVP
                     VGTSPQSFAGALKYLGAVKLVVATSFIPAHNELVRAFLISEGFDVLGIESLDTGMTTL
                     EKALLSPTQVFRHVRAVGRKYATCDAVLITSSAWPTLTVIQPLEDDLGKPVVSSSIGQ
                     IWWPLKELGIRAEINGYGKLLGTLS"
     gene            complement(1319..2935)
                     /locus_tag="VFN58_04665"
     CDS             complement(1319..2935)
                     /locus_tag="VFN58_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET8917316.1"
                     /translation="MSVKPVDCQRSFYHTRYLCGDLFGPASRYRLFREKIWPKLLELS
                     PKLHAFYCEDNGRPAVDPVLLCGVTLLQFMEKVADRGASEHVVYHLGWKYALDLELDY
                     EGFHSTVLVYFRDRLEEHKAERMIFDGVVKLLMELGLVKKKGKQRLDSTHVVGYVKAM
                     SWLECAMETLRLGLEDLEAEVGRKKPPEFWERLWGLYVQSKLDWRLSRTEHDGRYRQC
                     GQDMRELLEWIDRNDPKHNDREAVKVLRRVFEEQFEVVEGKLQPTQRRRPRSVQNPHD
                     PDAHYADKGTKQWIGYKVHVAESVDPERPIKKKGEPAEHFITEILTTEAAQDEMAGLT
                     ETLRREQEHHEITPQAMYTDAGYVTEHTLTQAEQSGMELLGPTRPDPHKGAYNTEGFH
                     VDIDKRQAVCPQGNLSTQCSRIKDNYMQTEYYRIEWSHQCDSCPVQKQCTRSKSGRRT
                     LVVGLRHDLVQRRREQMRQAGFAKTMHPRNGVEGTHSELVRGHGLRRTKYRGLSRISL
                     SHYLMGAACNVKRYLNLLAFQMRTAALSPT"
     gene            2999..3193
                     /locus_tag="VFN58_04670"
     CDS             2999..3193
                     /locus_tag="VFN58_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917317.1"
                     /translation="MNYPTKIQAIRRKKAPPQCYVNLPLPLAAAIDIEPGEMVEWKVD
                     SRYKLWLVRQRPKPKKNKKS"
     gene            complement(3571..>4134)
                     /locus_tag="VFN58_04675"
     CDS             complement(3571..>4134)
                     /locus_tag="VFN58_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET8917318.1"
                     /translation="YAGLAVSLPGYGATEVLPETDPKITLNVVLDAASLVKKLPWIDS
                     KRSYLYGFSRGAFFTSLLVNRIQGIEGIVLHSGAYDLNRLYQETPWFRSLLNPSGEEN
                     PKLISILPEVSTWHAPTLVLHGAKDSLVSVQQANLLRDSLETAKIPYRFVLYPNNGHR
                     LPVEDVRKKATAFLEESSGSACRNGAP"
BASE COUNT          956 a         1187 c         1008 g          983 t
ORIGIN      
        1 agtccggtgc ccatgccgac caaccgcaca tcgagaccgc gcttttcgaa gagtttagat
       61 tccttggcaa accacaaggg cgcgatggcg ccgtccgtag aaccatagcc ggcgacgatt
      121 ttgatgagcg gcttggcctg cgccgccaat gcagttggag cgtcaacgtc aagaaaacca
      181 gcgatgatcg ccgcaattag caaactgatg ctacttttga tcctcatgac actgcctccg
      241 tcaggagagc gtcccaagaa gtttaccgta cccgttaatc tccgctctta tcccgagctc
      301 ctttaacggc caccagatct ggccgattga gctgctcacc accggcttgc caagatcgtc
      361 ctcgagcggc tgaatcacgg ttaacgtggg ccacgccgac gaagtgatca acaccgcatc
      421 gcaagttgca tattttctgc cgaccgctcg cacgtggcga aaaacttgcg ttggcgagag
      481 cagcgctttt tctaaggtcg tcatgcccgt gtcgagcgat tcaatgccta acacgtcaaa
      541 accttccgaa atcaggaatg ccctcaccaa ttcgttatga gcgggaatga atgacgtggc
      601 gaccaccagc ttcacggcgc cgagatattt tagcgcgccc gcaaatgatt gcgggctcgt
      661 cccgacgggc accggaaaac ggcttctgaa ttcctgcaaa agctcccggt gccggtcgac
      721 accgttagcc aataccagtg gtgcgccgct gagggtgata tggtccactc gcgtgtgcaa
      781 gaggtcttca aaggcggtat agaacctacg ccaagaagcc tcgacttggc ttgactcccg
      841 cgcgtcgcga atcaccagat tggtcgttac gaaagtgact ccctcgggag ccacccgata
      901 aaactcatac ggcaggatct cagcacaagt cgtcggtgaa atgttgccga tccgcgcgcg
      961 ccatccgtac attaagcctc catcgcacct ttgccgccgt gaataccact cttctgcgcc
     1021 gaaagtattc gttgacgcgc ctgctgtcaa cttgaactga cctgtatgcc gacaagacta
     1081 ttgacagaaa agtcttcgcc gcaatctttg acgaaatgac tttaatcacc aaggggaatc
     1141 atctcgtagc gcaaaactag gattgtaatg attgttatgt catgtaatga ttgtgattct
     1201 gctgaagaac ccccattttt tatttctgat cggcgcgaag aatgctttcg attcaaatac
     1261 gaaggttgcg acagacaact tcgatcctaa ctcgcgaaaa atgaactgct cttagccctt
     1321 aagtggggct cagagcggcg gttctcatct ggaaagccag tagatttagg tatcgtttca
     1381 cgttgcaagc tgcccccatc aggtaatgcg atagactaat tcgactcagc ccccgatatt
     1441 ttgtccgtct aaggccgtgc ccgcgcacca actcgctgtg ggtcccctcg accccgtttc
     1501 gcggatgcat cgttttggca aaccccgcct ggcgcatttg ttctcggcgt ctttgtacca
     1561 gatcgtggcg cagtccgacc accaaagtcc tgcgtccact cttggaacgc gtacactgtt
     1621 tttgtactgg acagctgtca cattgatgac tccactctat gcggtagtac tcggtttgca
     1681 tgtagttatc tttaatgcgg ctgcactgag tgctcaagtt gccctgagga caaaccgcct
     1741 gacgtttgtc gatatcaaca tggaacccct cggtattgta cgctcccttg tgaggatcgg
     1801 gacgcgtcgg ccctaacagc tccatcccgc tttgctctgc ttgcgtcaag gtatgctcgg
     1861 tcacataacc cgcatccgta tacatcgcct ggggggttat ctcatggtgc tcctgttctc
     1921 ttctgagcgt ctcggttaaa cccgccatct cgtcttgggc cgcctcggtc gtcaggatct
     1981 cggtaataaa atgctcggcg ggctcgcctt ttttcttgat gggccgttct ggatcgacac
     2041 tctccgccac gtgcaccttg tagccgatcc actgcttggt ccccttatcg gcgtaatgag
     2101 catcgggatc atgagggttc tgtaccgagc ggggccgcct cctttgagtg ggctgaagct
     2161 ttccctccac cacctcaaac tgctcctcaa agacccgccg caacactttc accgcctcac
     2221 ggtcattgtg cttaggatca tttctgtcta tccattcaag cagctctctc atgtcttgcc
     2281 cacactggcg atagcggccg tcgtgctcag tcctgcttaa ccgccaatca agtttgctct
     2341 gcacatataa cccccacagt cgttcccaaa actcgggggg ctttttcctc ccaacttcag
     2401 cttccaaatc ctccaacccc aaccgcagcg tctccatcgc gcactccaac cagctcatcg
     2461 ctttgacata tcccacaaca tgggtgctat cgagccgctg cttgcccttt ttcttcacca
     2521 accccagttc catcagcaac ttaaccaccc cgtcaaagat catccgttcg gctttgtgtt
     2581 cctcgagccg gtcgcgaaaa tagactaaca ccgtcgaatg aaacccttcg tagtccagtt
     2641 ccaaatccaa ggcgtacttc caacccagat gatagactac gtgctcgctg gctcctcggt
     2701 ccgccacttt ctccataaac tgcaacagtg tcaccccgca caacagcacc ggatcgacgg
     2761 ccggtcggcc gttgtcctcg cagtaaaacg catgaagctt cggtgaaagc tctaaaagct
     2821 tgggccagat cttttcccga aacagccggt agcggctggc cgggccaaaa agatccccac
     2881 aaagataacg ggtatgataa aagcttcgtt ggcaatccac aggttttacg ctcatgcgcc
     2941 gagaataaaa gcgaacccgt cgcttgtcaa cgtctatttt attaactaga gtgataaaat
     3001 gaactacccc accaaaatcc aggccatccg ccgcaaaaaa gctcctcctc agtgctatgt
     3061 caacttgcct cttcccctgg ccgccgccat cgacatcgaa ccgggagaga tggtggaatg
     3121 gaaagtggat tctcgttaca aactctggct cgtccgccaa cggcctaaac caaaaaaaaa
     3181 caaaaagtca tgagggtttt tcagcagaat catattggtt attcggagga atctgcaggt
     3241 cacggcattt acctagctgg ttgattgcac catggactca ttcatcgagg cgattgcaaa
     3301 ccgcctttca cccatgcaat agccagttcc aatcgttcca gccgttcaat cgctatgctc
     3361 cgttcattac cgggctcaat ccgttccagt cgtttaatgt tttgaagggg tccgtgtggt
     3421 cctagcattg caggtagttg aagctattca atttaatagg catcggtaaa cagagataag
     3481 gccaggggtc agatcttgta ctatgtaatt ggttgtggtg gggagcggaa gttaaaaagg
     3541 cacaaaagac gaagtagctt attgagcaaa tcaaggagcg ccattccggc aggccgatcc
     3601 ggagctttct tcgagaaagg cggtggcttt ttttctgacg tcttctaccg gcaggcgatg
     3661 cccgttattg ggatagagaa caaaacggta gggtatcttg gcggtctcga gggagtcgcg
     3721 taagagattc gcctgctgga cggaaaccag agaatctttt gcaccatgca gcaccagggt
     3781 cggagcgtgc cacgtggaga cctccggcag gatactgatg agcttcgggt tctcttcccc
     3841 gctaggattc aacagggagc gaaaccatgg tgtttcttga tagagccgat tcaggtcata
     3901 cgcccccgag tgcaagacta tcccttcgat tccctggatc cgatttacta gcagcgaggt
     3961 gaagaatgcg ccacgcgaga agccgtaaag gtaagatcgc ttcgaatcta tccacgggag
     4021 cttcttgaca agggaagccg cgtctaggac gacatttaag gtaattttgg ggtctgtctc
     4081 cggcagcact tctgtcgcgc cataaccggg aagcgaaacg gccaaaccgg cata
//
LOCUS       DASSWZ010000191         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_162099, whole genome shotgun sequence.
ACCESSION   DASSWZ010000191 DASSWZ010000000
VERSION     DASSWZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162099"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1073)
                     /locus_tag="VFN58_04680"
     CDS             complement(72..1073)
                     /locus_tag="VFN58_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917319.1"
                     /translation="MKTETKRATEGLVLQYGVTLAVFLWIGFFGRGEAVAQKVRVAMP
                     AKSMTFLNFYVGDKFGVYKAEGLEVSLEVIKTEVGVAGMVAGEIDYTSAIGSAMRAAA
                     TGVPIKATLFSMDRVLIYMFARPTIKSIEELKGGKTVATTGLVATPTYAAKVMARAHG
                     LNADKDLTFIATGDAANALAALQSGAADVAMLSIPFNFKAEELGFRNLGSAVDYLQTP
                     FAGVGAADAKLKTNPAQVKRMIRATLKGIEFTKDPANQERVTGLLMDEFKLDRKTAAL
                     SLREIIKVFTRDGTIPEDAVKAEIREIREQAKLKGEIPVTQLVDYRLLEEVLADTKR"
     gene            complement(1153..1917)
                     /locus_tag="VFN58_04685"
     CDS             complement(1153..1917)
                     /locus_tag="VFN58_04685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8917320.1"
                     /translation="MKLSAKIACVTGSVRGIGWEIVQAYAQEGAQVVICDLHQADVDL
                     ALARLGLPLERVLGVTADVTVEEDVVYLFRAIRERFGRLDILVNNAGFAWPREGPIDL
                     ELADTPFQVWLKVLNTNLTGTFLCSREALRLMRPQRFGTIINISSPQGKLGRALRGPY
                     SAAKFGVEGLTQVMSLENSSHDIRVNALDPGGIVATEAIRKIPGNKGKKMLSPEIVRA
                     CAVYLASDESLGVTGRSFVATEWNEQHGIEVPYMVA"
BASE COUNT          494 a          675 c          550 g          551 t
ORIGIN      
        1 cggagctcat ctttgcggaa cagattgttc aatccgggga tgatttatta cagcaagaga
       61 tctgtaatct tctagcgctt tgtgtctgcc aggacttctt cgaggagcct gtaatcgaca
      121 agctgggtta ccggaatctc gcccttgagt tttgcctgtt cgcggatctc cctgatctcg
      181 gccttcacgg catcctccgg gatcgtccca tctctggtga aaaccttgat gatctctcgc
      241 agggagagcg ccgcagtctt gcggtcaagt ttgaactcat ccatcaatag gccggttacc
      301 ctttcctgat ttgccggatc cttggtaaat tcgatcccct tgagcgtagc gcggatcata
      361 cgttttacct gggctgggtt ggtcttgagc tttgcgtcgg cagccccgac tccggcaaag
      421 ggcgtctgga gataatcgac cgcgctgcca agattccgaa accccagctc ctccgccttg
      481 aagttaaagg gaatggaaag catggccaca tctgctgctc cactctgcag ggcagccagc
      541 gcattggctg cgtcgcctgt ggcaatgaat gtcaagtcct tgtcggcgtt gaggccgtgg
      601 gcgcgcgcca ttaccttggc cgcataggtc ggcgtcgcaa ccaggcccgt ggtagccacg
      661 gtcttcccgc ctttcagctc ctcaatcgac ttgatcgtcg gccgggcgaa catgtagatg
      721 agcactctgt ccatgctgaa cagggtggct tttatcggca cccccgtggc ggcggcgcgc
      781 atcgccgaac ctatggcgct tgtgtagtcg atctctccgg caaccatacc ggccacgccg
      841 acctcagttt ttatgacttc taaagagacc tctaaacctt cggctttgta aacgccgaac
      901 ttgtctccta catagaagtt gaggaacgtc atgctcttgg ccggcatggc gacgcgtacc
      961 ttctgtgcca cggcctcacc cctcccgaaa aacccgatcc agaggaagac ggcaagtgtt
     1021 actccgtatt gtaaaaccaa tccttcagtc gctcgcttgg tttcggtttt catgcttcac
     1081 tccttcgaga gtgctgcttg ccacagctcg ggagtattga cgagaaccaa tactccatca
     1141 ctccgctctt tactacgcaa ccatatacgg aacttcgatc ccgtgctgtt cattccattc
     1201 agtggcaaca aacgaacggc cggtcacccc cagggattcg tccgatgcga ggtagacggc
     1261 gcaggctctc acgatttcag ggctaagcat cttcttccct ttgttgccgg gaattttgcg
     1321 tatggcctca gtcgctacaa tccctcccgg gtcaagggca ttaaccctta tgtcatgcga
     1381 gctattctcc agagacatga cctgcgttaa cccctccacg ccgaacttgg cggcggagta
     1441 cggtccccgc aacgctctcc ccagttttcc ctgcggggag gatatattga tgatcgttcc
     1501 aaacctctgg ggcctcatca atctcagtgc ctctctgctg cacaaaaagg tcccggtaag
     1561 attcgtgttg agcaccttga gccagacttg aaaaggcgta tccgccagtt ccagatcgat
     1621 tgggccctct cgaggccagg caaagccagc gttgttcacc aggatgtcca gcctgccgaa
     1681 tctttccctt atcgcgcgga acaaataaac cacatcttcc tcaaccgtaa catctgcggt
     1741 cacgcctaaa acgcgttcca acggcaagcc caaccgcgcc agagctaggt caacatcagc
     1801 ttgatgaaga tcacaaataa caacctgagc tccttcctga gcataggcct gaacaatctc
     1861 ccacccgata cctcttaccg atcccgttac acaggctatt ttggcgctta gcttcatcgg
     1921 ttcctagtct ctgtggaccc cctgagtttc ccaaagccgc tgtagaacgt cacatgatga
     1981 agacaaccag ttgcaccact ctgaaaatcc tagccatcaa cgaaaactag tcgattttct
     2041 ttcttctaat ctggctggtg agtttatcgc ttaacagaac ctggagatcg acacaatcaa
     2101 aaacttgccc agcccgattg aaacgcctga cccaagaaag actgctgaat tgaaatcatg
     2161 ggtcgaggag atcgcattgt gtgacgggaa aagggcggtc ttctttggca tgcaaaccac
     2221 cgttgaccct cgaagtcgcg ccaatgtctc catcttgcac tggtttgatg
//
LOCUS       DASSWZ010000192         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_869828, whole genome shotgun sequence.
ACCESSION   DASSWZ010000192 DASSWZ010000000
VERSION     DASSWZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869828"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFN58_04690"
     CDS             complement(<1..221)
                     /locus_tag="VFN58_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HET8917321.1"
                     /translation="MSQPLIDHRGPTFAALLREVLQGLKALFRTAEGHVLLFPGSGTG
                     GWECCIVNTLSPGDQVLGCVNGYFSDLFCR"
     gene            complement(378..1118)
                     /locus_tag="VFN58_04695"
     CDS             complement(378..1118)
                     /locus_tag="VFN58_04695"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET8917322.1"
                     /translation="MAQESQGFSLTWKVITGLVSMIILLGLLIIGVVYHLTSSALRDQ
                     FDRRAFAIATNLSDAAAAHVVRKNALELAGLVRKYALLQGVEYAFIEDGKGEIIAHTL
                     GTFPPELKKNVSTNGRLQQQSRSLQLGEKLVYETSVPILGGQVGAAHLGMRGDFVEGE
                     IRRAILPMIGIMAAILLGGILISSVLVGVMTRPIRHLRHVADNMSKGDLDTPVSIGIE
                     SHDEIGDLARSLERMRSSLKAAMSRFSG"
     gene            complement(1159..2523)
                     /locus_tag="VFN58_04700"
     CDS             complement(1159..2523)
                     /locus_tag="VFN58_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917323.1"
                     /translation="MDRLKYQKKLSWLAGFLSALGLVFPTLARAADQPAGGRLEKVRI
                     AYSAISGSQLVGWIANDAGFFRKNGLDVEFIFIEGGPRAVQSLASGEVQFALVAGAPV
                     IQSNLRGLPVVLVTGYLNTLDYQFIVDKAITRPEQLKGKAVAVSQPGSSSDFAMRYAL
                     DKYGLEPGKDVSLVEIGSQPARFAALQSGKIQGVMIAVPLTLTAKKMGFNMLADLQML
                     GLEYQYTAMATTRDMIKSRPDLVRSVVRASVEAIHYLKTHRKESIDILRKYMKTDDTE
                     ALAETYEAIALNLVPERPYPTLRGIQIILQELAPKDPKAKAARPEQFVDMSFVKELDA
                     SGFIDRLYKAKPVVAGGGTPQPPAPPSSTGKGVSTVQKKELVSDAKRTPEKLAQTSKT
                     SSGSTEHTVRAGDTLSHLALQYYGNARKWQQIYEANKETMKNPNYIYIGQKVLVPTDT
                     QGPA"
     gene            complement(2908..>3428)
                     /locus_tag="VFN58_04705"
     CDS             complement(2908..>3428)
                     /locus_tag="VFN58_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917324.1"
                     /translation="FAGGGTAEQAWDQLQRTGRVEWVNDLAEIPAQYGVNNVYGDLGQ
                     IFAQSTMADPRVCAFMMGTMIKGMGADHVVWGSDAVWTGSPQWQIEALRRLEIPDEMQ
                     KKHGFKPLGPADGQVKRAILGDNNARLYNFTPEQRSALENDRFAEIRENYKKDGEGRS
                     NRTYGYILKPTA"
BASE COUNT          771 a          970 c          895 g          792 t
ORIGIN      
        1 cgacaaaata ggtcgctgaa gtagccgttc acacagccca gcacttggtc gccgggcgaa
       61 agcgtgttga cgatacagca ttcccatccg ccggtgccgg aaccggggaa gaggagaaca
      121 tgaccttcgg ctgtccgaaa gagcgccttt agtccttgca aaacttcgcg cagaagggcc
      181 gcgaaggtgg ggccccgatg atcgatcagg ggctgggaca tggcccgaac aacccgctct
      241 ggcacgatgc tcgggcctgg tatctgaaga aagggacggc cgggcataag atgcgcgaac
      301 gctatctaat ctttgccggt catgtcaagg gtgttggctt aaagctccag cgacttggaa
      361 cctcgaaatc actatgacta tccactgaag cgtgacatcg cggctttcaa acttgaacgc
      421 atccgttcta gggaacgggc caggtctccg atctcatcat gtgactctat gccgatgctg
      481 accggtgtat cgagatcgcc cttgctcatg ttgtccgcca cgtgcctcaa atgtcggatg
      541 ggccgagtca tcacccccac aagcacagaa gagatgagga tgccaccgag gagaatagcg
      601 gccattatcc caatcatagg gagaatggcg cgtcggatct ctccctcaac gaaatctccc
      661 cgcattccca ggtgcgcggc gcccacctgt ccaccaagaa tcggaacgct agtctcatac
      721 actagcttct ccccgagctg caacgatcga ctttgctgct gaagtcgccc attggttgaa
      781 acgtttttct taagttctgg tggaaaagtg ccgagagtat gggcgataat ctctcccttg
      841 ccgtcctcga taaaggcgta ctccactccc tggagaaggg catacttcct gacgaggccg
      901 gcaagttcta aagcattctt tcttacgacg tgtgccgcag cggcatcact tagattcgtg
      961 gcaatcgcaa aagcccgtcg gtcgaattga tcccgaagcg cactgctggt gaggtgatag
     1021 acaacaccga taatgagcag gcccaagagg atgatcatag acacaaggcc cgtgatgacc
     1081 ttccatgtca gactaaagcc ttgggactct tgagccactt tattgccttg tgaaactagc
     1141 cttaagagca attgatcgtc atgctggccc ctgggtatcg gtggggacaa gaaccttttg
     1201 gccaatgtat atatagttcg gatttttcat agtttcctta ttggcttcgt aaatttgctg
     1261 ccatttgcgt gcattgccat agtactggag tgccaagtgg ctgagagtat ccccagccct
     1321 cacggtatgt tctgttgatc cggaagacgt cttggaggtt tgagctagtt tttccggcgt
     1381 tcgtttggca tcagaaacga gctctttctt ttgaacagtt gacactcctt ttcctgtgct
     1441 gcttggggga gcagggggtt gcggcgttcc accaccggca acaaccggct tagctttata
     1501 cagccgatcg atgaatcccg aagcatcaag ttccttgaca aagctcatat ccacaaactg
     1561 ctcgggccgg gctgccttag ccttagggtc tttcggggct agctcctgaa gtataatctg
     1621 aattccgcgc agggtaggat acggtcgctc gggaaccagg ttgagtgcga tggcttcata
     1681 ggtctctgcc agcgcctcgg tgtcatcggt cttcatatac ttccgaagaa tgtctataga
     1741 ttctttgcga tgagtcttaa gatagtgaat ggcctctacg gaggccctaa ccacactgcg
     1801 aacgaggtcc ggccgggact tgatcatgtc tcgggtggtt gccattgcgg tatactgata
     1861 ttcgagcccc agcatctgga gatcggccag catgttaaag cccatctttt ttgccgtgag
     1921 ggtcagcgga accgcgatca tcaccccctg gatcttgcct gattgaagcg cggcaaagcg
     1981 tgccggctgg ctgccgatct cgaccaggct cacatcctta cctggctcca gcccatattt
     2041 gtcgagagcg tagcgcatgg caaaatcgga ggaggatcct ggctggctta ctgccacagc
     2101 ctttcctttg agttgctcgg gccgggtgat cgccttgtca acaatgaact ggtagtcgag
     2161 ggtattaaga tatcccgtca ctaagaccac cggtaaaccc cgaagattac tctgtataac
     2221 aggggcgccg gctaccaagg caaactgcac ctcgccggag gcgagcgact gaacggcccg
     2281 gggtccgccc tcgataaaga tgaactcaac atccaaaccg tttttgcgga agaaccctgc
     2341 gtcattagca atccacccca ccagttggct gcccgaaatg gcgctgtagg ctattctcac
     2401 cttttcgagc cgacctcccg cgggttgatc tgctgctcga gccaacgtag ggaaaacgag
     2461 cccgagcgcg gacaggaatc cggcaagcca ggaaagtttt ttttgatatt tcagccgatc
     2521 catatttcct cctcaatcgt aaatcagcgt caggcaatga gtttattgtt caaaccagac
     2581 ttctccgtgc cccaaaacaa aaagtccggc tgggctaccc caagtccagc ctcccgcaga
     2641 aattatcgaa gatgcgacct gattgcctga cccagccgaa attagatggg gtaaaatagg
     2701 gcaggacgcc tacagggctt caagggaaaa tagctttcct tcttctatat ctcaatactc
     2761 ctcacttctc caatacctcc ttcacaacgc gattcttccg gctttggagg atgactcgcg
     2821 cgctgaggat cgaccccaat catcatatat atcagtggac cagaagaacg gcaccaccga
     2881 gcgccttcaa tggttccgaa gaaaagacta tgccgtaggc ttaaggatgt aaccgtaggt
     2941 gcggttgctg cggccttcgc catccttctt ataattttct ctgatctccg caaaacgatc
     3001 gttttcgagc gccgaacgtt gctccggggt gaagttgtaa agccgggcat tgttgtcacc
     3061 aaggatcgcc ctcttaactt gaccatccgc cggtcccagc ggcttgaacc catgcttctt
     3121 ctgcatctcg tcgggaatct cgagccgccg taatgcttcg atctgccact ggggcgaccc
     3181 ggtccagacc gcatctgacc cccacaccac atgatcggcg cccataccct tgatcattgt
     3241 gcccatcata aaggcgcaca cacgcgggtc ggccatagtg ctctgagcaa agatctgccc
     3301 gaggtcgcca tacacgttgt tcacgccata ctgcgcgggg atctcggcga gatcgttgac
     3361 ccactcgacg cgtccggtcc gctgcaactg gtcccaggcc tgttctgcgg tgccgcctcc
     3421 ggcgaagc
//
LOCUS       DASSWZ010000193         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1393159, whole genome shotgun sequence.
ACCESSION   DASSWZ010000193 DASSWZ010000000
VERSION     DASSWZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393159"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /locus_tag="VFN58_04710"
     CDS             complement(<1..2273)
                     /locus_tag="VFN58_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8917325.1"
                     /translation="MEKLLLDIQFAKPLFLWLLLGLPLLWVRVRDQRLLVILARTIIL
                     LLLICGLADPQWVTRQARQEERIFAFDISQSVTNSMRHWMGATAEGRLAPTHQDRVFV
                     FASGTKEASDWREWLKKDGAHPEVIQPMKTDLEKLFTTLLALPPAPRSLFLFTDGWET
                     QGNVEALLPAIAASGLKIFPVIPTEQPKIDNVTVKRLVIPNQGKSGEALNLRVILENQ
                     SEGEVEGTLTLTRNNQSLKSETVKLKPGSQIYTYQTTLPDNPVTSYRAQFTPRQAELD
                     INLADNQAVAWVAVKTKAKVLLLNGQSSGGRYLEEILKRQGFEVTSRTADSPPSPAGY
                     GVVIFNNVERSRFSSNYLVAIERHTAQGNGFLMLGSDASFAPGSYSRTPIEAILPVEP
                     KEPKREEKNRAVILVIDKSGSMREENRILYAQEAAKAVARQLKDNDLLGIVAFDVTPF
                     VVVPLEPVGKLRGVIDAQINRLKPGGQTYFYPALIEAKRQIERANAARKHVILLSDGE
                     TRGTQGELVDLVTVMKNEMKVTVSAIAIGAEADVRVMKRISQYGGGLFHQTIDPTSLP
                     QIVLAQLQDNPSEEPRGERELTPIQERGSELLAGFSVRSYPRLFGYMETELKRGAHLD
                     LMLPREDRKIPLLASWRYEKGRVAAFTADLEGRWTKNWIQWSALQTFWDKLLGWLRPS
                     DERDTIPVHEARVGLSANKPFLDLFLYEDTGADGQFRFSVNGKGSKAEGVLQKLAPGH
                     YQAALPLAAPGDYRVELT"
     gene            complement(2291..>2968)
                     /locus_tag="VFN58_04715"
     CDS             complement(2291..>2968)
                     /locus_tag="VFN58_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917326.1"
                     /translation="DNPLVDLEKPVSRPAVSSWREPHPLTRYVNFALLRPSFSRPLRP
                     RVSGETIIESPAGPLVFATAQRGVRQLALGFDIFPYLGRENLPVSIFTLNLLDWFFHG
                     SVEGRVTGAPLTFTSTQQGNLLLTPRGDKMTLHPGTNNFPATYWQGVYQLDRGAQREL
                     FAVNLKNINESDLRQPTPIELRDISSEGSKVSALFSIWPYLLLTSLFLLLIEWFVNPR
                     PLRTRAG"
BASE COUNT          651 a          799 c          827 g          691 t
ORIGIN      
        1 gtcagctcaa cacgataatc tcccggagcc gctaaaggta gagcggcctg gtaatggcca
       61 ggggccagtt tttgtagtac tccctctgcc ttgctcccct ttccattaac tgaaaaccga
      121 aactggccgt ccgcaccagt atcctcgtag agaaacaaat ccagaaaagg tttgttggcg
      181 gaaagaccca ctcgcgcctc gtgaacgggt atggtgtcgc gctcgtcgct gggacggagc
      241 caaccgagga gcttgtccca aaacgtttgc aaagcactcc attggatcca gttcttggtc
      301 caacggcctt ccaagtccgc cgtaaaggct gcgactcttc ccttctcata tcgccaggac
      361 gccaataagg gtattttccg gtcttctctc ggaagcatga gatccagatg agcccctcgc
      421 tttaactcag tctccatgta accaaagagc cttggatagg atcttactga aaagcctgca
      481 agtagctctg atcctctttc ttgaatcggg gtcagctccc tttcacccct gggttcctcc
      541 gaaggattgt cttgcagctg ggccaggacg atttgcggca gggaagtagg gtcgatagtc
      601 tgatggaata gtccgccgcc atattgacta atccgtttca tgactcgcac atcggcttcc
      661 gcgccgatgg caatggccga aacggtgacc ttcatctcgt tcttcataac ggttacgaga
      721 tcgaccagct cgccctgcgt gcctcgggtc tctccgtcgc ttaacagaat gacatgcttt
      781 cgcgcggcgt tggctcgctc gatctggcgt ttggcttcga taagcgcggg atagaagtag
      841 gtttgtcctc caggtttaag gcggttgatc tgagcatcga ttacgccacg gagcttgccg
      901 acgggttcca gtggtacgac cacgaaaggg gtaacatcga aggccactat cccaagcaga
      961 tcattatcct tgagttggcg cgccacggcc ttagcggctt cctgagcgta caggatacgg
     1021 ttttcttcgc gcatgctacc ggacttgtcg ataaccagaa tcacggctcg gttcttttcc
     1081 tcgcgctttg gttcctttgg ctccacaggg aggatggcct cgatgggcgt gcggctatag
     1141 cttccagggg cgaagctggc atcgcttccg agcatcaaaa aaccgttgcc ttgagctgtg
     1201 tgccgctcga tggcaacgag atagttggag gaaaatctgc tccgctcgac attgttgaag
     1261 ataacgactc cataaccggc cggcgatggt gggctatcag cagtacgtga ggttacctcg
     1321 aaaccttgcc gtttgagaat ctcctctaaa tagcggccgc cgctgctttg cccgtttagc
     1381 agcaaaacct tcgccttcgt ttttactgct acccatgcga cagcttggtt gtccgctaag
     1441 ttaatgtcga gttcggcttg tctgggggta aattgggctc gataagaggt gaccggattg
     1501 tcgggaagcg tcgtctggta ggtataaatc tggcttcccg gtttaagttt gacggtctct
     1561 gacttcagac tctggttgtt gcgtgtcaag gtcagggtcc cctcaacttc accttcgctt
     1621 tggttttcaa ggatcactcg caaattcagg gcttcgccgc tctttccctg attggggatc
     1681 accaatctct tgacagttac gttgtcgatc ttgggctgct ccgtcggaat aaccgggaaa
     1741 atcttgagcc cggaggccgc aattgcgggt aacaaagcct ccacattccc ctgggtttcc
     1801 cagccgtcgg tgaacaaaaa aaggctgcgc ggtgcgggtg ggagagccag caaggtcgta
     1861 aacagctttt ccaggtcggt cttcatcggc tgaatgacct ctggatgggc gccgtccttc
     1921 ttgagccatt cacgccagtc cgaggcttct ttggtaccgg aggcaaaaac gaagacgcga
     1981 tcttggtggg taggggcaag ccttccctcg gccgtggctc ccatccagtg acgcatgctg
     2041 ttagttacgc tctgcgaaat gtcaaaagcg aaaattcgct cttcttgcct agcctgtcgg
     2101 gtaacccact gggggtcggc gagaccgcaa atcagcagca aaagaataat ggttcgtgcc
     2161 agaatgacga gcagcctctg atcacggacg cgcacccaga ggagcggcaa cccgagcagc
     2221 agccagagaa acagcggctt ggcaaactga atatctaaga gcagtttttc catgaaccct
     2281 gaggattatc tcagccggcg cgcgtccgca gcgggcgcgg attgacgaac cactcgatca
     2341 acaagagaaa caatgacgtc aatagtaggt aaggccagat agaaaatagc gccgacacct
     2401 tgctgccctc actactaatg tccctgagtt caatgggagt tggttggcgc agatcggact
     2461 cgtttatgtt cttgaggttg acggcgaaaa gctccctctg agcgccccga tcgagctggt
     2521 atacgccttg ccaataggtg gccggaaaat tattggtccc tggatgaaga gtcatcttat
     2581 cccctcttgg agtaagtagc aggtttcctt gctgcgtaga ggtaaaggta agcggcgcgc
     2641 cggtaactct gccctccact gaaccatgaa agaaccagtc caggagattg agcgtaaaga
     2701 ttgagaccgg caggttttct cgccctaagt aagggaaaat atcgaagccc agggccagtt
     2761 ggcgaacccc gcgctgcgcc gttgcaaaga cgagtggtcc cgcggggctc tcgatgattg
     2821 tctcgccgga tacccgtggc ctcaatgggc gtgaaaatga aggccgcaac agcgcgaaat
     2881 tgacatagcg ggtcagtgga tggggctcgc gccagctaga cacggcgggt cgagaaacgg
     2941 gcttttccaa atcgaccaac gggttgtc
//
LOCUS       DASSWZ010000194         5782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_374636, whole genome shotgun sequence.
ACCESSION   DASSWZ010000194 DASSWZ010000000
VERSION     DASSWZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374636"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFN58_04720"
     CDS             <1..479
                     /locus_tag="VFN58_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917327.1"
                     /translation="SFEITELVGRQDKNPYYKVRFNSGKEGFIHPEVFLEELNLSITS
                     VDPQAIDRKKAAEAAEEEKKRVAWIQAQPWPQTFKEAAIKRKVMPGMSTGEVRQILGK
                     PVSVSKFKAQYNLAGENWLYPDGSTVVFHSGQMVRVEGKPNTEPKPSTEPKPSEEE"
     gene            complement(516..1007)
                     /locus_tag="VFN58_04725"
     CDS             complement(516..1007)
                     /locus_tag="VFN58_04725"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET8917328.1"
                     /translation="MEIIIDGYNVIGSDTGLAGNLEHKRNSLVQQLASYHKNKGHGVT
                     VVFDGWRSGSTDEVKQKRDGICIVYSRLGEKADSVIVRLARKQGAGCVVVTSDREVRS
                     AVERFGTVALYTGEFREILRNLAESFDDQESDYPQQGAPKKGNPRRLSKKERKRREKL
                     NKL"
     gene            1128..2294
                     /locus_tag="VFN58_04730"
     CDS             1128..2294
                     /locus_tag="VFN58_04730"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET8917329.1"
                     /translation="MTIQKAKRIKELPPYLFAEIDRRKRAALARGVDLIDLGIGDPDI
                     PTPAQVVETLIESASKPTNHRYPNSSGLIEFREAVANWYQQRFNVKLDAGKEVVSMIG
                     SKEGIGNMAVAFVDPGDVVLVASPCYPVYHIGTGFNGGKNYFLPLRKENHFLPDLDSI
                     PVEVAKQAKLLWINYPNNPTAAVADKAFFKRVVDFANRYNVIVCHDAAYTEMGYDGYR
                     PMSFLEVEGAKEVGIEFHSLSKTFNMTGWRIGMAVGNPDLVEGLAQAKSNLDSGIFQA
                     IQEAGIAALKLGDKIVEPSRKIYQERRDILVDGLRAVGLECEKPRATFYVWVSTPKGL
                     SSAEFTTKLLEQAGVVTTPGNGFGEAGEGYVRFTVCIDKSKLKEVAERIRQVKL"
     gene            2318..2821
                     /gene="folK"
                     /locus_tag="VFN58_04735"
     CDS             2318..2821
                     /gene="folK"
                     /locus_tag="VFN58_04735"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET8917330.1"
                     /translation="MANAMAHQVYMGIGSNVGNKKENFFEALSRLAKLPDTKIVKESS
                     LYESEPLGDSKDWYVNGAIEIETKFKPDMLLKKFKNIERAMGRKKVKKRWGARIIDLD
                     ILLYDALVVKKKTLRIPHPEMPNRKFVLVPLSEIAPQVIHPELGVTISELLVNVKDEK
                     KVHLYHS"
     gene            complement(3078..4493)
                     /gene="mpl"
                     /locus_tag="VFN58_04740"
     CDS             complement(3078..4493)
                     /gene="mpl"
                     /locus_tag="VFN58_04740"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HET8917331.1"
                     /translation="MNNLPPPGSRVHLIAICGVGMASLAGLLQSRGYRVTGSDQNVYP
                     PMSTYLQGVGIPILSGYSAAHLQPRPDLVVIGNAISRNNPEADAVLSHGIPYISFPQA
                     LGLFLIQSRTSLVVAGTHGKTTTSALVAWVLARAGLDPGFFIGGVPLNFSGGWNPGSG
                     DYVVLEGDEYDSAFFDKGPKFLHYRPHNVILTSVEFDHADIYRDLDHLKDAFRRLVEL
                     IPSSGCLLACNDYEAVRDVVGAAHCRLLYYGGQSSADWTANNIAFRDGKSLFEPFYRG
                     RSDGVVELGLIGRHNVQNALGVYAMGRELGIDRDKLCDGLATFAGVKRRQEIKGNIGG
                     VLVMDDFAHHPTAVKETLSGVIEAYPGRRLWAIFEPRSNTSRRNIFEQEFAQALALAD
                     RIVIAGLHQPEKVPEPERLSVEKVVRAINGVGEPRAVAIEKADSIASYVAENAVPNDI
                     VVVMSNGGFDGVQDKILQALA"
     gene            complement(4490..5617)
                     /locus_tag="VFN58_04745"
     CDS             complement(4490..5617)
                     /locus_tag="VFN58_04745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET8917332.1"
                     /translation="MDFKSVEDAFKEAVTQGVFPGAVLLVGRGDEIVYEHAFGSRSLV
                     PTSSPMHTSTIFDLASLTKPLATTVAIMLLVSEKKLSLDDRVTLFFPTFGIFDKHAVT
                     FRHLLNHSSGLSDWKPYYEDVLKSEKAGKLNFIASRAAKQYVFAQIHREKLVSPTGVR
                     SLYSDLGFIILGEVVEAIGGGTLDRFCQDRIFTPLGLRSMFFIDLTQLDTQRFQPVEE
                     MIAPTEDCPWRKRVLCGEVHDDNAYAMGGVAGHAGLFSSARDIHQLLACLDGCRRGEN
                     TFLPQALIQEFVSRDKSVKNSTYALGWDTPSENGSSSGKYFSAHSVGHLGFTGTSIWW
                     DLEKNSHVVLLSNRVHPTRKNEKIREFRPYIHDLIMKTLNP"
BASE COUNT         1442 a         1551 c         1488 g         1301 t
ORIGIN      
        1 aatcgttcga aattacagag ttggtgggtc gtcaagataa aaacccttat tacaaagtca
       61 ggtttaattc tggaaaagaa ggtttcatac atccggaagt ttttctggaa gaactaaact
      121 tgagtatcac gtccgtcgat ccacaggcga ttgatagaaa aaaagccgcg gaagcggctg
      181 aggaagaaaa gaagagagtt gcgtggattc aagcccagcc ttggccacag actttcaagg
      241 aagcggccat aaagcgtaag gtgatgcccg gtatgagtac gggagaggtg agacaaattc
      301 tgggtaagcc cgttagtgtc agcaagttta aagctcagta caacctcgcc ggagaaaatt
      361 ggctctatcc ggacggctca accgttgttt tccacagtgg acaaatggtc cgcgttgaag
      421 gcaagcctaa tactgaaccg aaaccaagta ctgaacccaa accaagcgaa gaagaatgat
      481 taggcgaccg tcgatacgtg cgccgttagt caagattaca gtttgttgag cttctctcgc
      541 cgctttcttt cttttttcga caatcgtctt ggatttcctt tcttgggcgc cccttgctgc
      601 ggatagtcag actcttgatc atcgaatgac tcggcgagat tgcgcaggat ctccctgaat
      661 tcgccggtat acaaggcgac ggtgccgaag cgctcaaccg cgctccttac ttctcgatcc
      721 gaagtcacga ctacacaccc agcgccctgt tttcgtgcga ggcggacaat cacgctatcc
      781 gccttctctc ccaagcggga atagacgatg caaattccat cgcgtttttg tttaacttcg
      841 tcggtcgacc cggatcgcca gccatcaaag acaaccgtga cgccatggcc tttgttttta
      901 tggtagctgg cgagctgctg cacgagcgag ttccgcttgt gctcaagatt accggctaat
      961 ccggtatcac taccaatcac attatagccg tcaatgatga tctccatcgc tccataggag
     1021 attaaccgcg aagcgcccaa aatagaagct tttggggaag tagttgtcaa aatcgaaaaa
     1081 aacaggtaaa tactgggcga ttttagcggg agggtgtgga gttgggtatc accatacaaa
     1141 aagccaaaag aatcaaagag ttaccgcctt accttttcgc ggaaatcgat cgcaggaaac
     1201 gtgcagcttt ggcccgcggg gtcgatttga ttgatctcgg aatcggcgat ccagacattc
     1261 cgactccggc tcaggttgta gaaaccctca tcgaaagcgc cagtaaaccg accaatcatc
     1321 gttatcctaa cagttcggga ctgatcgagt ttcgcgaggc agtggcaaac tggtatcagc
     1381 aacggttcaa cgtcaaattg gacgccggta aggaagtcgt gtcgatgatt ggatccaaag
     1441 agggcatcgg caatatggcg gtggctttcg tggatcccgg cgacgttgtt ctagtcgcca
     1501 gcccgtgtta tccggtttac cacatcggca ccgggttcaa cggcggcaaa aactactttc
     1561 tgccgttaag gaaagagaat cattttctcc cggacctcga ttcgattcca gtagaggtcg
     1621 caaaacaagc caagcttctg tggatcaact atccgaacaa tccaaccgct gccgtagcgg
     1681 acaaagcatt ctttaaacgg gtggtcgact tcgccaacag gtacaatgtg atcgtctgcc
     1741 acgacgcggc ctacaccgag atgggttacg acggctatcg gcccatgagt tttctcgaag
     1801 tcgaaggagc aaaagaagtc ggtatcgagt ttcactcact gtccaaaact tttaacatga
     1861 ccggctggcg tatcggcatg gccgtgggga atccggatct ggtggaaggc ctcgcgcaag
     1921 ccaaatccaa tctggactcc ggcattttcc aggccattca agaggccggc atcgccgccc
     1981 tgaagcttgg cgacaagatc gtcgagccct cccgaaagat ataccaggaa aggcgtgata
     2041 tcttggtcga cggtcttcgt gccgtcggcc ttgaatgcga gaaacctcgt gcgaccttct
     2101 acgtttgggt ttctactccc aagggtcttt cgtctgcgga gtttacgacc aaattactcg
     2161 aacaagccgg tgtagttacg actccgggaa atggctttgg cgaggcggga gaagggtacg
     2221 tgcgctttac ggtctgtatc gacaaatcaa agctaaagga ggttgcggaa agaatccggc
     2281 aggtcaaact ctaggtttct tttcgccgcg gggcacaatg gcaaatgcta tggcccatca
     2341 ggtctacatg ggcatcggct ccaacgtggg caacaagaag gaaaatttct tcgaggccct
     2401 cagtcgatta gccaaacttc cggacaccaa gattgtcaag gagtcatcgc tttacgagag
     2461 tgaacccctg ggagattcta aggattggta tgtcaacggc gctatcgaaa tcgagacgaa
     2521 gtttaagccc gacatgctcc ttaaaaaatt caaaaacatc gagcgcgcca tgggccgcaa
     2581 gaaagttaag aagcgttggg gtgcacgcat tatcgacttg gacatcctgc tatacgacgc
     2641 gcttgtcgtg aagaaaaaaa ccttgcgcat tccccacccc gagatgccga atcgaaagtt
     2701 cgttcttgtc ccgctcagcg aaatagctcc ccaagtcatc catccggagc tcggggtgac
     2761 gatttctgag cttctcgtga atgttaaaga tgagaaaaag gtccatctgt accattccta
     2821 agtaaccatc cgtctggacc tccagggcga agccattgct aattcgcgtt ctacttttcg
     2881 tcttcctcgt atatatcttt taccttgcgc tcaaggccta ttggttgccc cgtaagagcg
     2941 acccccgttt cgtgaactca cgaagagttg acgacatggt tctcgatccg caatgtcaga
     3001 cctacgtccc gaagaatgaa gccatccccc agaagggcaa ctatttctgc agtcgggaat
     3061 gcgccaggct ttttctgtta cgctagcgcc tgcagaattt tgtcttggac gccgtcaaat
     3121 ccgccgttgg acatcacgac aacgatgtca ttgggaacgg cgttttccgc cacataggat
     3181 gcgatagaat ccgctttttc aatggcaacg gcacgcggct ctccaacgcc attgattgcc
     3241 cgaacgactt tttcgaccga cagccgttca ggctccggaa ctttttccgg ttgatgtagt
     3301 ccggcgatca cgatacggtc cgccagcgcc aaagcctgcg cgaattcctg ttcgaagata
     3361 ttccgtctgc tcgtgttgct tcgcggctca aagatcgccc acagtctccg cccaggatac
     3421 gcctctataa cgccgctcag ggtttctttg accgctgtgg ggtgatgggc aaagtcatcc
     3481 atgaccagca caccgcctat gttgcccttg atctcctgcc gtcgcttgac cccagcgaac
     3541 gttgccagac catcgcataa cttgtctcgg tcgattccca actctctacc catggcgtaa
     3601 accccgagcg cgttttgcac gttatggcga ccgatcagac cgagctcgac aacgccatcg
     3661 gatcggcctc ggtaaaaggg ctcgaaaaga cttttcccat cccgaaatgc aatgttattc
     3721 gccgtccagt ccgctgaaga ttgcccgccg tagtacagga gccggcaatg cgccgctccg
     3781 acaacgtctc gtactgcttc gtagtcattg caagcaagca ggcagccgct cgatggaatg
     3841 agctcaacca gccggcgaaa tgcatctttc agatgatcga gatcacgata aatatcagca
     3901 tggtcaaact ccacgctggt aagaatgacg ttgtgcggcc gatagtgcag aaacttgggg
     3961 cctttgtcga agaaggcgct gtcatactcg tccccttcta aaacgacata gtctccgctt
     4021 ccaggattcc atccgccact gaagttgagc ggcacaccac caataaaaaa acccggatcc
     4081 aaaccggcac gtgcaagcac ccatgcaacc agagccgatg tggtggtttt cccgtgggta
     4141 ccagcgacca ccaaggaagt ccgcgattga atgaggaaaa ggcccaaagc ctgcgggaat
     4201 gaaatatagg ggatgccatg gctcagcacg gcatcagctt cgggattgtt ccgtgatatt
     4261 gcgttgccga tgacgacaag atcgggccga ggctgcaggt gcgctgcact gtagccagac
     4321 agaatcggaa tcccgacccc ttgaaggtaa gtgctcatcg gcgggtaaac gttttgatcg
     4381 gagccggtga cacgatatcc ccgggactga agcaggccgg caagggaagc catgcctacc
     4441 ccgcaaattg cgatcaagtg aacacgactg ccgggcggtg gaaggttgtt catggattca
     4501 acgttttcat gatcaagtca tgaatgtatg gacggaactc tctgatcttt tcattcttgc
     4561 gtgtaggatg gacccggttt gatagcagca caacgtggga attcttttcc agatcccacc
     4621 aaatcgaggt tccggtgaaa ccaagatgac ccacagaatg ggccgaaaaa tactttccgc
     4681 tggaagaacc attttccgac ggcgtatccc agcccagcgc ataggtagag tttttcacag
     4741 atttgtccct cgagacaaac tcctggatca acgcttgagg caaaaatgtg ttttcgccgc
     4801 ggcgacagcc gtcgaggcaa gctaagagtt gatgaatgtc ccgtgctgac gaaaaaagcc
     4861 cggcatgacc cgccacccca cccatcgcgt aagcattatc gtcgtgcacc tcgccacaga
     4921 gaactctctt gcgccaggga cagtcctcgg tgggggcgat catctcctcg accggctgga
     4981 accgctgggt gtccaactgc gttagatcaa taaagaacat gctcctgagg ccaagaggcg
     5041 tgaatatcct gtcttggcag aagcgatcta gtgtgccgcc gccaatagcc tctacaactt
     5101 cgccaagaat gatgaaaccg agatcgctgt agagactcct tacgccggtc ggactgacca
     5161 gtttctcacg atggatctgc gcgaacacgt actgtttagc ggcccggctt gcgatgaagt
     5221 tgagctttcc tgccttctcg cttttgagaa cgtcctcgta atacggcttc cagtccgaca
     5281 gccccgagga atggtttagt aaatgccgga acgtcaccgc gtgcttatcg aagattccaa
     5341 aggtggggaa aaaaagagtc acccgatcgt cgaggctaag cttcttttcg ctcacgagca
     5401 gcataatggc gacggtggtc gccagaggtt tcgtcaaaga agccagatca aagatcgtgc
     5461 tcgtatgcat gggactgctc gtgggaacga gagaacgcga gccgaacgcg tgctcataga
     5521 caatctcgtc tcccctaccc acgagcaaca cggctccagg aaacactcct tgagtcacag
     5581 cttctttaaa ggcgtcttcg accgacttga agtccacggt tttattttga agctacggcc
     5641 taaaaaattc cggaaagtta gctctcgcga gaaaatctgc gaatcacgcc actggcgatt
     5701 ccatcagcga caaggtcccc gcgtctccat cgagtgccag ctccacgcca aagggcaatg
     5761 ccagattttc tcgtccgtgc cc
//
LOCUS       DASSWZ010000195         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1011572, whole genome shotgun sequence.
ACCESSION   DASSWZ010000195 DASSWZ010000000
VERSION     DASSWZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011572"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1116
                     /locus_tag="VFN58_04750"
     CDS             274..1116
                     /locus_tag="VFN58_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET8917333.1"
                     /translation="MPAIRQILTKVNSLNGAQKRLGHYLQTDSSALLLSNVGDLARAV
                     GVSKATVVRFAKSLGYKGFPEFRREIQQEMRRKLRAADRMEETFAELENDENIFAKLI
                     KRDIQLLEETLQAASFPDFHKAVEIILRARKVFLIGLNASMALAYLLHFRLVRVKKEA
                     HWIFLTGGTSLLEQLAFMDAGDVLIAIDFLQVPREVQTALQHAKKVGAPILGITDFPS
                     SAIAKAADVCLYAKRGLHTTVNSLTPAFSLVNALAIAVAWTKKADSIKALTDLDKLLE
                     GHSP"
     gene            1151..2146
                     /locus_tag="VFN58_04755"
     CDS             1151..2146
                     /locus_tag="VFN58_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8917334.1"
                     /translation="MATKLKFGLLLPHFCEYGSTELCIEGSKKAEAYGFDSVWVRDHL
                     VFEPHGMEGEDTTHIEGLLILAAISSVCKNLILGTGTVISHRHPIHLAQSMAGLSTMC
                     DNLIMGLGLGTFPHEFEAAGYKKVTLQDRANMAKINAELCRRFWSGEKISYKDDYYDF
                     KDVELKPTPKNNIPIWYGGGTPASCRRAADYCDGWMPGRIPTATFNKMVKYLEEQCKL
                     AGRPMVTTGAIPITSIDKNRDVALSKVNVKGLINEGNTPAKKTWVRPKSGTFSTVEDI
                     EGLLMAGTPADIARDTKRYEEAGLNHIVFDLRFRYADWYQQIDLLGQEVLPAVRA"
     gene            2264..>2474
                     /locus_tag="VFN58_04760"
     CDS             2264..>2474
                     /locus_tag="VFN58_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S15"
                     /protein_id="HET8917335.1"
                     /translation="MRTEKVNFYSEGNKLAGVLYLPQSDQGKPFPGIVQGPGFLGLKD
                     AKHYIMMFEKLCEAGYACLCFDYRGW"
BASE COUNT          600 a          665 c          657 g          552 t
ORIGIN      
        1 tccttgcgct tccaatattc gagcttctcc gtgcgcacat cctgcacaaa agcgccgccg
       61 atgatcggca cgccttcgga ctcgatccac ttcttgtagg gactgtattt gtccatcatg
      121 aactgaacat agggatgaac ctcgaattct tttgttggtc ctgccatgtg acctcctttg
      181 cgcgtatgac ttatggcaaa ctatcgcgag ggaaaaaacc tgtcaactat cttgacaaga
      241 aagtttcgct gcgataggtg aaacaaaagt ttcatgcctg cgattcgaca gattttaacc
      301 aaggttaact ccctaaacgg ggcgcaaaag cggctcggcc attacttgca aaccgacagc
      361 tccgctcttc tgctctccaa cgtgggcgat ctcgctcgag ccgtaggcgt cagtaaggcg
      421 accgtggtgc gcttcgccaa gagcttgggc tacaaaggtt tcccggagtt caggcgagag
      481 attcagcagg aaatgcgccg caaactgcgc gctgccgacc gaatggaaga gaccttcgcc
      541 gagttggaga acgacgagaa catatttgcc aagctgatca agcgcgacat ccaactcctc
      601 gaggaaacac ttcaagcggc ttcgtttccg gacttccaca aagccgtgga gattatcttg
      661 cgcgcgcgga aggtgttcct catcggtctc aacgcttcta tggcgctggc ttaccttctc
      721 catttccgcc tcgtgcgcgt caaaaaggaa gctcactgga tctttctcac cggcgggact
      781 tcgctcttgg agcagctcgc ctttatggac gcgggcgacg tgctcatcgc tatcgacttc
      841 ctgcaagtgc cgagggaggt ccagaccgcg ctgcaacacg ccaagaaagt aggcgcgccc
      901 attttaggaa tcaccgactt ccccagctca gctatcgcca aagccgcgga cgtctgcctg
      961 tacgcgaagc gcgggctcca caccactgtg aattcgttga caccggcttt ttctttagtc
     1021 aacgctctgg cgatagccgt tgcatggacc aaaaaagccg attcaatcaa agcattaacc
     1081 gatctcgata aattgttgga agggcatagc ccataatcca agtgcgattt tcaggaaagg
     1141 agttgtttaa atggcaacaa agctaaaatt cggactactg ctgcctcact tttgtgagta
     1201 cggttcgacc gaactctgta tcgaaggatc gaaaaaggcc gaggcttatg gcttcgattc
     1261 ggtgtgggtg agagaccact tggtttttga accccacggc atggaagggg aggatactac
     1321 ccacatcgag gggttgctga tcctcgcggc gatctcgtcg gtatgcaaga acctgattct
     1381 cggcaccggc acggtgatct cccatcgcca cccgattcat ttggcgcaat cgatggccgg
     1441 gctcagcacg atgtgcgaca acctcatcat gggtctcggc ctcggcactt ttccccatga
     1501 attcgaagcg gcgggttata aaaaggtgac tttgcaggac cgcgccaaca tggcgaaaat
     1561 caacgccgaa ctctgccgcc ggttctggtc cggtgaaaag atcagctaca aagacgatta
     1621 ttacgatttc aaagacgtgg agctaaagcc gacgccgaag aacaatattc cgatctggta
     1681 cggcggcggc acaccggctt cctgccgccg cgcggcggat tactgcgacg gctggatgcc
     1741 gggacgtatt cccacggcta cctttaataa gatggtcaaa tacctcgaag agcagtgcaa
     1801 gctggcggga cggcccatgg tcaccaccgg tgcgattccg atcaccagca tcgataagaa
     1861 cagagacgtc gcgctcagca aggttaacgt caagggtctg atcaacgaag gcaacacacc
     1921 cgccaagaaa acttgggtga gaccgaaatc tggcaccttc tccacggtcg aggacatcga
     1981 agggctgctc atggcgggaa ccccggcgga catcgcccgt gatacaaaac gatacgaaga
     2041 agcgggtctc aatcatatcg ttttcgacct gcgcttccgc tacgccgatt ggtaccaaca
     2101 gatcgacttg ttggggcaag aagttctgcc ggcggtgcgg gcataaacgc cgttcaaatc
     2161 gttccaaacg ttcgagtcgt tcaagatgtt ctgaagattg gcattgacga ttggaacgat
     2221 tgaaactatt ggaacagttc cagtttcaac tgaggagtca gtcatgcgaa cagagaaagt
     2281 aaatttttat agcgagggca ataaactcgc tggcgtgctt tacctgccgc agtcagatca
     2341 gggaaagccc tttcctggaa tcgttcaggg acctggtttc cttggattga aggatgcgaa
     2401 acattacatc atgatgttcg agaaactttg cgaggccggt tatgcctgtc tctgcttcga
     2461 ctatcgcggt tggg
//
LOCUS       DASSWZ010000196        10712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_134147, whole genome shotgun sequence.
ACCESSION   DASSWZ010000196 DASSWZ010000000
VERSION     DASSWZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134147"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..282
                     /locus_tag="VFN58_04765"
     CDS             1..282
                     /locus_tag="VFN58_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET8917336.1"
                     /translation="MNKRFPHLSRRDSEVIINAIFDSMVDALAQGDRIEIRGFGSFVV
                     KHRRARQGRNPKSGVLVSVAAKRVPFFKVGKELRLRVDGKDPGTEEASE"
     gene            279..770
                     /locus_tag="VFN58_04770"
     CDS             279..770
                     /locus_tag="VFN58_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET8917337.1"
                     /translation="MKQLWAPWRMTYINQVSNAEGCIFCQKMAEKDDREALVLAHTGH
                     SVVMLNKYPYANGHLLLAPKRHEKHLSQLSADEYGDLNEALRRSVDIVRKSYNPAGVN
                     LGMNLGKCAGAGVEDHLHWHVVPRWEGDNNFMPVLAETRVMPQHLLESYDQLKPYFQH
                     LSQ"
     gene            874..2274
                     /locus_tag="VFN58_04775"
     CDS             874..2274
                     /locus_tag="VFN58_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HET8917338.1"
                     /translation="MNEAKTLGELKKSGVGVLSVRDEMRKNLVHRLETGTRILPGIVG
                     YDDTVIPEIENAILSGHHMVFLGERGQGKSRIIRGLTSLLDERIPIVQGCEINDNPFK
                     PICRACRKKAEEVGDGLPLRWIDRDERYGEKLATPDVSIADIVGEIDPIKVAEGRYLA
                     DEETIHYGLVPRTNRGIFAINELPDLTEKVQVGLFNLMEEKDVQIKGYKIRLPLDVVI
                     VASANPEDYTSRGRIITPLKDRFDVQIRTHYPKSIDDELVIMEQEAAPIDQRSRKVEV
                     PRFLKEILGHLTFEARKSNEINQSSGVSVRVTINNFESLVSNAEKRSLRCKENEIVPR
                     VSDLHAILASTSGKIEMEYVGEDKKEDDLVEKLLNRSIVKVFDQYFSLNSLQKVVEYF
                     NSGWGVEVSDQMASQDYLQDIKEIPGLKEAIRSLGIQDSPSLMASATEFVLEGLHLHQ
                     KLNKEIEGGRVTYGNK"
     gene            2383..3912
                     /locus_tag="VFN58_04780"
     CDS             2383..3912
                     /locus_tag="VFN58_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8917339.1"
                     /translation="MFDQLNEYMNDSGDLQQAMRRLMQKGIKRGENQTKGLDDLLSQV
                     AREMRKLYDEYRLQSAMDEVQEQLESIVDQERHTLEELDQSGQDTQEKKQFLNNLPGK
                     ASEAVEKLTSYRFENANAEKEFQQLLMQLEQIRKLENWLRREGSLFRGQTPLQFQESM
                     DLMERMEELRRLEGQLSSMQLRDVDKELLEKLLGGDPKQDFEGVMRMQSLLEEGGYVL
                     EQGDRFELTPRGIRRVGQLALRDIYQQLRRDGMGRHSARYRGSQEIQIENSRPYVQGD
                     ALHINMIQTLKNALLNGGGVPVKIHPGDFAVYDSEHTTRAATVLLLDMSWSMSWEGRF
                     AAAKKVALAMESLVRSLHPRDYFGIVGFFTRAVELKPKDLPQATWNMGDPFTNLQDGL
                     HLAAEMLERRPSPNQQMIVITDGQPTAYCRQGRLYCEWPLSFGGISQRAAEETLKEAE
                     RITRKGITINTFMLDDSPVLRGFVDDLTRINRGRAFYTRPDRLGQYLLIDYLSHQKKK
                     V"
     gene            4118..4726
                     /gene="lexA"
                     /locus_tag="VFN58_04785"
     CDS             4118..4726
                     /gene="lexA"
                     /locus_tag="VFN58_04785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET8917340.1"
                     /translation="MLSSLTLRQKEIYDFLLRTIREKGYAPSIPEIGRQFKIASTNGV
                     SDHLKALEKKGYIRRVGKRAIEVLSAVGKPVLTAVRDIPIVGRVPAGKPFLSEENIEG
                     LLTITNDMGSGKLFALQVKGDSMIGAGILDGDRVIVKQQGTVENGEIVCALIEGEATL
                     KRFFKKDGVITLKAENEKYAPITVSTGEFRVLGKVVGLMRKF"
     gene            4786..5205
                     /locus_tag="VFN58_04790"
     CDS             4786..5205
                     /locus_tag="VFN58_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET8917341.1"
                     /translation="MPVKDFEGLEIWKEARRLTCEIYDLSKTPTFSKDFGLRNQMQRA
                     AVSIMSNIAEGFERGGNQEFVQFLYIAKGSCGELRSQLYVALDQVYIDHKVTDSLLVI
                     LKRLSVMIKHLIDHLKRSGMRGPKYGDSPRSSYSETS"
     gene            5279..5878
                     /locus_tag="VFN58_04795"
     CDS             5279..5878
                     /locus_tag="VFN58_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HET8917342.1"
                     /translation="MFSFPAPELRRDIVHPSPWNLAAIAGRFIEISASASAASLTLAF
                     GLVGEAQKQREPVGWVTSTGSFFYPPDAAHGGVDLAALVVIRVLHAASIPVAGEKLLR
                     SGGFGLIVLDLGTADIPIPIQSRLTGLAQRHHAALVCLTEKENKTFSLGSLVSLRAHA
                     ERQRNSDHRFACRLRVLKDKRHGPTWSHEELCHGPAGLC"
     gene            5856..7361
                     /locus_tag="VFN58_04800"
     CDS             5856..7361
                     /locus_tag="VFN58_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET8917343.1"
                     /translation="MVRLACVDLPAFPLQLLLRRHPEWASYPAAVVAEDKPQGLILWV
                     NEKGRQLGVLPGLRYAAALSLAAGLRAGEVAPSEIHNAVKNLTRRLMRFTPEVEPSSE
                     EPGVFWLNGRGLRLLYPSSTKWAEALHADIAAAKFQISVVAGFTRFGTYAVAKVKKGI
                     TIFNNPAEERQAAHKVPLHRLDLDPDFRDTLFKLGIKTVGALLSLPAVGLRERFGPKA
                     YRLHRMAAGDLWMPLNPYQPEEPVRQHRILDDPENDTTRLLFLIKQLLHPMLATLAAR
                     AQALVVLWLSFLIERDGWLKEQIRPAAPTLDSSQILDLVRLRLESMQFSAGVAEIALE
                     AEACAATAEQLRLFSEGPARDLDAANRALARLRTEFGDDAVVQAKLANGHLPEARFTW
                     EPLSRVKLPRNDDLNVLNCPAAKVLVRRMMAKPIPLPGGPRHTHEDGWLILGHKYGSV
                     DKLSGPYVFSGGWWNREIQRDYYFAETRRGAIAWVYYDRVRRRWFLQGFIE"
     gene            7488..7925
                     /locus_tag="VFN58_04805"
     CDS             7488..7925
                     /locus_tag="VFN58_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET8917344.1"
                     /translation="MTIQNLPTIHPGRILLEEFLEPRGISVWHLAKDLGVSVRRIYEI
                     VQSQRPITGDIARRLAHYFGMSERFWLDLQARYDGQRDQPAAKEVSGKQEPRYDGLPK
                     REAAVLKILNRPRPVSRTLVVVDGGLSVKTQVETSSSTKPKLL"
     gene            7973..8124
                     /locus_tag="VFN58_04810"
                     /pseudo
     CDS             7973..8124
                     /locus_tag="VFN58_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683314.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8739..8975
                     /locus_tag="VFN58_04815"
     CDS             8739..8975
                     /locus_tag="VFN58_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917345.1"
                     /translation="MTWQVSADAKRPACEILPETGRLTRDPPWPCLYTHRGHIHLRSM
                     WVDIRNAAKRYSEDVADQQLLARFVECMKRLYPT"
     gene            9342..10484
                     /locus_tag="VFN58_04820"
     CDS             9342..10484
                     /locus_tag="VFN58_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007190997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8917346.1"
                     /translation="MKQTASTVKDQVMHKDSNQLPIELSIVVPTFNEKDNVEKLIERI
                     EQALPDRKWEMIFIDDDSPDGTADFVRRLAQSKPYVRCHQRIGRRGLSKALIEGILSS
                     SAPVIVVMDADLQHDETILPTMLDRIRNDQTEIVVGSRYCAGGSIGDWNSQRAAMSRL
                     ATLLSRTIVTPHLTDPMSGFFMLRRETFHRIVRRLSGEGYKILLDLFASSPEPLRFVE
                     VPYTFRERTAGESKVDTAVAWEYLLLVADKKFGHILPARFILFSLVGLSGVVVHFLAL
                     ATTFKFLGLQFSIAQTIATLSAMTSNFAFNNILTYRDMRLRGSRFLTGLFTFYLVCGI
                     GVIANVGVANFVFQRDYTWWLAGGAGALVGTVWNYAASSIFTWGRQ"
BASE COUNT         2588 a         2749 c         2975 g         2400 t
ORIGIN      
        1 gtgaacaaaa gatttccgca tctttctcga agagattcgg aagtgatcat caatgctatc
       61 ttcgattcca tggtggacgc tcttgcgcag ggcgaccgca tcgaaatacg cggctttgga
      121 agtttcgtcg taaaacaccg gcgagcacgt caaggacgaa acccgaagag cggggttctg
      181 gtgtccgtgg ctgccaaacg cgtcccgttt tttaaagtcg gtaaagaact aaggctgaga
      241 gttgatggca aagacccggg caccgaagag gcctcggaat gaagcagctc tgggcaccat
      301 ggaggatgac ctatatcaat caagtctcaa atgcggaagg ctgtatcttt tgtcaaaaaa
      361 tggcggagaa agatgaccgc gaggctttgg tcctggctca taccggccat tccgtggtaa
      421 tgcttaacaa atacccttac gccaatggcc accttcttct tgctcccaag cggcacgaaa
      481 agcatctttc tcaactctca gcagacgagt acggcgatct taatgaagcc ctgcgccgtt
      541 ccgtcgatat cgttcggaaa tcctataatc cggccggtgt caatctgggc atgaatctag
      601 gcaagtgcgc tggcgccgga gtcgaggacc atctccattg gcatgttgta ccgcgatggg
      661 aaggggacaa taattttatg cccgttctag cggaaacccg ggtgatgccg cagcacttgc
      721 ttgaaagcta cgaccagcta aagccttatt ttcaacatct ctctcaatga aacaaaaaaa
      781 gcagccaaga ttgaaaatct ttgaagtcgc tccatctaca agaaaccatc gcctaggggc
      841 gtgaaaaatt cttaccaagg aggccatgtc caagtgaacg aggcaaaaac gttaggggaa
      901 ctcaaaaaaa gcggggttgg ggtattatcg gttcgcgacg agatgcgcaa aaatcttgtt
      961 catcgcctgg aaaccgggac gcgcattttg cccggaattg tcggttatga cgataccgtg
     1021 attccggaga tcgaaaacgc gatcctttcc gggcaccata tggtattcct cggcgaacgg
     1081 ggtcaaggga aatcgcgaat tattcgcggc ctcactagcc tcctcgacga acgcataccc
     1141 atcgttcaag gctgtgagat caacgacaac cctttcaaac ccatttgccg ggcttgtcgg
     1201 aagaaagccg aggaagtggg cgacgggttg ccgctccgat ggatcgaccg agacgaaagg
     1261 tacggggaaa aattggcgac gccggacgtc tccatcgccg atatcgttgg tgagatcgat
     1321 ccgatcaagg ttgccgaggg aagatacctc gccgacgaag agacgattca ctacgggctc
     1381 gttccgcgca ctaatcgcgg catcttcgct atcaatgagc ttcccgattt gacggaaaaa
     1441 gttcaggtgg gtttgttcaa cctgatggag gagaaagacg tccagattaa gggctataag
     1501 attcgcttgc ctttagacgt cgtgatcgtt gccagcgcca acccggagga ctataccagc
     1561 cggggacgga ttatcacgcc gctcaaggat cgcttcgatg tccaaatccg cacccattat
     1621 ccaaagagca tcgacgacga actcgttatc atggagcagg aagcagcgcc gatagatcaa
     1681 cggagccgca aagtggaagt accgcggttt ttgaaggaaa tcctcggcca cttaactttt
     1741 gaggcccgga agtccaatga aatcaatcag tcgtcaggcg tgagcgtcag ggttacgatc
     1801 aacaattttg aaagcctggt cagtaacgcg gaaaaacgtt cgctccgatg caaagagaat
     1861 gagatcgtcc ctcgggtcag cgacctgcat gcgatcttgg cgtcgaccag cggtaaaatc
     1921 gagatggaat atgtcggaga agacaaaaaa gaagacgacc tggtggaaaa acttctgaat
     1981 cgttcgatcg taaaagtttt cgaccaatat ttcagcttga actcattgca aaaggtcgtc
     2041 gagtacttca actccggttg gggcgtcgaa gtctcggacc aaatggcgtc ccaagattac
     2101 ctgcaagaca tcaaggaaat tccaggcttg aaggaagcta tccgctccct gggaattcaa
     2161 gactcgcctt ccctcatggc gtcggcaacc gagtttgttt tagaaggtct tcacctgcac
     2221 caaaagttga acaaggaaat cgaaggggga agagtcacct acggtaataa gtagcagcac
     2281 ggactagcaa tcgaattcga atttcgtccc ccatccccta tggtacacat aagatacagc
     2341 cgttgggaca ggagcacacg gaactctcta gattccgaca ccgtgttcga tcagctcaac
     2401 gaatatatga acgacagcgg cgaccttcag caggcaatgc ggcggctgat gcagaagggc
     2461 atcaagcgag gggaaaatca gaccaaggga ctcgatgatt tgttgtctca agtggcgcgg
     2521 gaaatgcgca aactctatga tgagtatcgc ctccagtccg cgatggacga agtgcaggaa
     2581 cagctggagt ccatcgtcga ccaagagcga cataccttag aagagctgga ccaatctggt
     2641 caagataccc aggaaaaaaa gcagtttttg aacaacctgc cgggcaaagc cagcgaagcg
     2701 gttgaaaaac tgacttctta ccgcttcgaa aatgccaatg ccgagaaaga gttccaacag
     2761 ttgctcatgc agttggagca gatccgcaaa ctggaaaact ggctgcgtcg cgaggggagt
     2821 ctctttcgcg gccaaacccc cctgcaattc caggagtcga tggacctgat ggagcgcatg
     2881 gaggagctcc ggcgtttgga aggccagttg tcgagcatgc agctgcgcga cgtcgacaag
     2941 gagcttttgg aaaaactcct gggtggcgac ccgaagcagg acttcgaagg cgttatgcgg
     3001 atgcaatcct tgctcgaaga aggcggctat gtcctggagc aaggagaccg ttttgagctg
     3061 actccgcgcg gtatccggcg cgtggggcag ttggccctga gagatattta ccagcagctg
     3121 cgccgggacg gcatgggcag gcattcggct cgttaccggg gcagccagga aatacagatc
     3181 gagaatagcc ggccctatgt ccaaggggac gcgcttcata tcaacatgat tcagactctt
     3241 aaaaacgcgc tgttaaatgg cggcggcgtg ccggtcaaaa ttcatcccgg ggactttgcc
     3301 gtctatgact ccgagcacac cacccgggct gccacggttc tgcttttgga catgagttgg
     3361 tccatgagct gggaagggcg attcgcggcg gcaaaaaaag tggcgttggc gatggagagt
     3421 cttgtccgat ctcttcaccc gcgagactat ttcggtatcg tcggcttttt cacccgtgct
     3481 gtggagctaa agcctaagga tctgccgcag gcgacctgga acatgggcga tccatttacc
     3541 aatctccaag atggcttgca tctggctgcc gagatgttag agcgccggcc gagccccaat
     3601 caacagatga tcgtcatcac ggacggtcag cccaccgctt attgccgcca gggccgcctt
     3661 tactgtgagt ggcctctgag ctttggcggt atcagccagc gggccgccga ggaaacctta
     3721 aaagaggccg agcgcattac ccgcaagggg atcacgatca acaccttcat gctcgacgac
     3781 agcccggtgc tgcgcggctt tgtcgatgat ctcacccgca tcaatagagg ccgcgccttc
     3841 tacacgcgac cggaccgttt gggtcaatac ctgctcatcg actacctctc gcaccagaaa
     3901 aaaaaggttt aatgcgcagg gcgcattaaa ccttttgttc caagctgttc caatagtttc
     3961 agttgttcca actgttccgg aggtcgattg agaaaatcaa gtcgttaagc agcttagttt
     4021 taacgactgg aactgctgaa acatttggaa cggctggaac ccgcggcttc tcgccgcggg
     4081 tattgacacc tgaaaataat ttcagtatat aggctaaatg ctttcttcgc tgactttaag
     4141 gcaaaaagaa atttacgatt tcttgctgag aacgattcgc gagaaaggct atgcgccttc
     4201 gattcccgag atcggcagac aattcaagat cgcttcgacc aatggcgttt ccgatcatct
     4261 caaggctctg gaaaagaaag gttacatccg cagagtcggt aagcgggcca tcgaagtttt
     4321 atccgctgtc ggcaagccgg ttctgacagc ggttcgcgac atcccgattg tgggccgggt
     4381 gcccgcgggc aaaccttttt taagcgaaga aaatatcgaa gggctattga ccatcaccaa
     4441 tgatatggga tcaggaaaac ttttcgcttt gcaggtcaag ggggacagca tgatcggcgc
     4501 cggcatcctg gatggcgacc gtgtgatcgt gaagcaacag gggacggtgg agaacggcga
     4561 gatcgtctgc gccctgatcg agggtgaggc gaccttgaag cgttttttta agaaagatgg
     4621 tgtcatcacc ctcaaagcgg agaacgaaaa atatgctccg atcaccgtct ctaccggaga
     4681 attccgcgtc ctgggcaagg tggtggggct gatgaggaag ttttagattt aaaaatagtt
     4741 tcaatcgttc catacgttcc aatcgttcca aacgttggag ggagtatgcc ggtaaaagat
     4801 tttgaaggct tggagatctg gaaagaagca cgtcgcttaa cctgcgaaat ctacgattta
     4861 tccaaaacgc caacgttttc gaaagacttt ggcttacgaa atcaaatgca acgcgcagca
     4921 gtttcaatta tgtcgaacat cgcagaagga tttgagcgcg gtggaaatca agaattcgtc
     4981 caatttctct atattgccaa ggggtcctgc ggtgagttgc gttctcaact ctacgtggcg
     5041 ttggatcaag tatacataga tcacaaggtg acggacagct tgctcgtaat tctaaaacgc
     5101 ttatcggtca tgattaaaca cttaatcgat catctcaaac ggagtggtat gcgggggccg
     5161 aaatatggcg attcaccccg atcgagttat tccgagacat cttgaacaat tggaacaact
     5221 ggaacggttg gaacctttgg aaccatcaaa attacgttga accttgaaca gtgggcctat
     5281 gttttccttc cccgccccag aactcagacg cgatatcgtt cacccgtcgc cgtggaatct
     5341 cgcggcgatc gccgggcggt tcatcgagat ttcggcttca gccagcgcgg cctcattgac
     5401 tctcgccttt ggccttgtcg gcgaagcgca aaagcagaga gagcctgtgg gctgggtgac
     5461 atcgacggga agcttttttt atccgcctga cgcggctcac ggcggagttg atcttgccgc
     5521 actggttgtt attcgtgtgc ttcatgctgc aagcatccct gtcgcgggag agaagctctt
     5581 gcgctcgggc ggcttcggtc tgatcgtgct cgatctcgga actgccgata ttcctatacc
     5641 gatccaaagc cgtctgaccg ggctcgccca gcgccaccac gcagccttgg tttgtcttac
     5701 agagaaggag aacaagacat tttcgctggg atcgctcgtt tcattgaggg ctcatgccga
     5761 gcgacaacgg aattccgatc accgttttgc ttgtagactc cgggttctca aagacaagcg
     5821 gcacggaccg acctggagtc atgaggagct ttgccatggt ccggctggcc tgtgttgatt
     5881 tgcccgcttt tccgcttcag cttttgctcc ggcgccatcc cgaatgggct tcttacccgg
     5941 cggccgtggt ggctgaggac aagccacagg gactgattct ctgggtcaac gaaaagggca
     6001 ggcaactcgg tgtgctgccg ggtctgcgtt atgccgcggc tctttctctc gcggcgggtc
     6061 ttcgagccgg tgaagttgca ccgagtgaaa tacacaacgc cgtaaaaaac cttacacgcc
     6121 ggttaatgcg tttcactccg gaagtggagc cttcctctga agagcctgga gtcttctggc
     6181 ttaatggtag gggtttaagg ctcctttatc cttcttcaac gaaatgggcc gaggcgctgc
     6241 atgcggatat cgccgcggcg aaatttcaaa tcagcgtggt ggcgggcttc acgcgcttcg
     6301 ggacttatgc ggtcgccaaa gtaaagaagg ggataacgat tttcaacaat cccgccgaag
     6361 agcggcaggc cgcgcacaag gttccgctgc atcgtttgga tctcgacccg gattttcgtg
     6421 acacactttt taaacttggg atcaaaaccg tcggtgctct gctttcactg ccggcggtgg
     6481 gattgcgcga gcgttttggg ccgaaggctt atcgtctcca ccgcatggct gccggcgatc
     6541 tctggatgcc gctcaatccg taccagcccg aggagccggt gcggcagcac cgtatccttg
     6601 acgatccgga aaacgacacc acgcggctgc tctttctcat caagcagttg ctccatccga
     6661 tgctggccac tctggccgcg cgcgctcagg cgctggtggt gttgtggctt tcttttctca
     6721 tcgaaagaga tggctggctg aaggaacaga tccgcccggc ggcgcccaca ctcgattcca
     6781 gccaaatact cgatctggtt cggttgcgtt tggagtcaat gcagttttct gccggcgtcg
     6841 cagaaatcgc gcttgaggcg gaagcatgcg cggccacggc cgaacaactc cgtcttttca
     6901 gcgaagggcc ggcgcgcgat ctggatgccg ccaaccgggc cttggcccgg ttgcgcaccg
     6961 aattcggcga tgatgcggtc gtgcaggcaa agttagcgaa tggacatttg cctgaagccc
     7021 gcttcacctg ggaacctctc agccgtgtca agcttccccg aaacgacgac ttgaacgttt
     7081 tgaactgccc ggctgctaaa gttttggtgc gccggatgat ggcgaagccc atacctttgc
     7141 ccggtgggcc gcgtcatacc cacgaggatg gctggctcat cttgggacat aagtacggca
     7201 gcgtcgataa actctccggc ccctacgtct tttccggcgg ctggtggaac agagagatcc
     7261 agcgcgacta ctacttcgcc gaaacccgcc gcggcgctat cgcgtgggtt tattacgatc
     7321 gggtgcggcg aagatggttt ttgcagggct ttattgaata aatcacagtt ccaaatgttc
     7381 caggagttcc aatcgtttca aacgttggag ggattatgcc cgtgaactct tgtggaacca
     7441 ctggaacgtt tggaaccatc ggaaccccaa caggataagg agcttacatg actatacaaa
     7501 atctaccgac gattcatcct ggcagaattt tgctggagga gttcctcgaa ccccgaggca
     7561 tcagcgtctg gcatttagcc aaagacctcg gtgtttccgt gcggcgtatc tacgaaatag
     7621 tccagagtca acggccgatc accggcgata ttgcgcgtcg actagcgcat tacttcggta
     7681 tgtcggaacg cttttggctc gacttgcagg cacggtacga cggacaaaga gaccagccag
     7741 ccgcgaaaga agtctcagga aaacaggagc cacgttacga cggtttgcca aagagagagg
     7801 cagcggtatt aaagatccta aacagaccta gacccgtcag ccgaacgctc gtagtcgtcg
     7861 atggcgggct gagtgtcaaa actcaagttg aaacgtcttc gagcaccaaa ccgaagctgt
     7921 tatgaaaggg cacgatccga aaccgataca attgcagaac ataaaacggg gtggtcgtta
     7981 tacgcttcga aattctttta caactcattt gctggaggaa ggcaacgaca tccgaacgta
     8041 taggaactac tcgggcacaa aaacgtaagc acgacaatct atacccatgc gttgaatcga
     8101 ggcggcaaag tcgtgcgcag ccctcgccga cggcctctag cccgtttgcc cgctatgcaa
     8161 gccatattgg atatacgaga ccagcgatat tggatatatg agcgaaaaga ggattgacaa
     8221 cttattgatt gaataagtaa atttcgcggt gggtctttta gaagtgcaat atcggtgatc
     8281 agtatactca caagttatac gtcagcatca tgagcagcag cggccaagca caagattcag
     8341 ttcagactaa gccagcacat cggggggcag ccgctgacat gccagcgttt gcagatcggt
     8401 tcatacaagc acggcttcaa ggctttgaga aagacatgaa ggtatgtctc acgccaatgc
     8461 gctctgaatc aagcaaagca cctacgcatg cttacttgcc ggcgctcgct gcttgctgcg
     8521 gccttcttga gtaccttaca ggtctttatc gcggtaggct tgaaggaaat ggctggcagc
     8581 agattgctga gtttgcagtt cgatacctgc ctcagcctga ctacgatgag gagacggtcc
     8641 ggattctttt ccaagttttt cggcacccga tagctcatcg cggaattgcg agcggaatct
     8701 gggtggatcg aaatcacggt ccaggaaagg gccggcgtat gacctggcaa gtctcggcag
     8761 acgcgaaacg acccgcctgc gaaattctcc cagagacggg gcggctcacc cgcgatcctc
     8821 cgtggccgtg cttatatacc catcgcggcc atattcatct tcgcagcatg tgggtggata
     8881 tccgcaacgc cgctaagcgt tacagcgaag acgtcgccga tcagcagctg ctggcgcgct
     8941 ttgtcgaatg tatgaagcgg ctctatccta cttgagagtg cagggaggtc atgacgtata
     9001 accccgcgtt cgagcggaca gtcgcaagcg ggcttcccac gcttgcgatg ccctcgtcgc
     9061 tatgctcctc gagtgccgct caacgcgaac gtttgggcat cacctaagga caagaagcga
     9121 atttgcggcg gcggttatta ttctaggagc ggatcgtccc ggcgaagaga atactttcaa
     9181 gttctatcaa ctggatgtca cgcttgagcc ggctgaggcg aagtagagat ggacgtgtct
     9241 aaccctgccg ttcaacccgg ccgcccatcg gtgcggcgct tcgcgctcgc cccatgagtg
     9301 atttagaggc agggcctacg gaattgtgat acattaattg catgaagcag accgcttcaa
     9361 ctgtaaaaga ccaagtgatg cacaaggatt caaatcagct tcccatagag ctctcgattg
     9421 tcgtgccgac gtttaacgaa aaagacaacg tggaaaaatt aatcgagcgc atagagcagg
     9481 cattgcctga ccgcaagtgg gagatgattt ttattgacga cgactctcca gacggaaccg
     9541 ctgattttgt gcgcaggtta gcccaatcaa aaccgtacgt tcgttgtcac caacgaattg
     9601 gtcgccgtgg tctttcgaaa gcgcttatcg agggaatctt aagcagtagt gctccggtga
     9661 tcgtcgtcat ggacgccgac ttacagcatg acgagacaat tcttccgacg atgttggata
     9721 gaatccggaa cgaccaaaca gagatcgtgg ttggaagccg ttactgcgct ggcggcagta
     9781 tcggcgactg gaattcgcaa agagcggcca tgagccggtt ggccacactt ttgagccgaa
     9841 ccattgtgac cccgcaccta accgacccga tgagcggttt ttttatgctt cgacgcgaga
     9901 catttcatcg gatcgtgcgg cgcctatccg gagaaggcta caagatactc ctcgacttgt
     9961 ttgcgtcgtc gccggaacct ctgcgctttg tcgaagtgcc ctacacgttt cgggaacgca
    10021 cggcgggaga aagcaaggtc gacacggccg tcgcctggga atatttgctg ctggtcgctg
    10081 ataagaaatt cggccatatt ctgccggctc ggttcatact tttttctttg gtgggactaa
    10141 gcggggttgt ggttcacttt cttgccttgg cgacaacttt caaatttctt ggtttacagt
    10201 tttcgattgc gcaaacaatc gccaccctga gcgcaatgac atctaacttt gcctttaaca
    10261 acatcttaac ctaccgcgac atgcgcttgc gcgggagtag atttctgacg ggattgttca
    10321 ccttttacct ggtatgcggc atcggtgtga ttgccaacgt cggcgttgcg aacttcgtgt
    10381 tccagcgcga ttatacctgg tggcttgccg gcggcgctgg agctcttgtt ggcacggttt
    10441 ggaattatgc ggcctcgtca atcttcactt ggggtcgcca gtgatacacg gtgatcagcg
    10501 gtcttgatgg ttgggcgcgc gcaggttaga tgatgtggta catctaaaaa gagacatcac
    10561 gaggatataa gaggagactc tatggcaatc cgactcgttg taacgatcac cgcagcgccc
    10621 ggaaagggct ctgagctggc gcaggcctac agggcgcgct gcgcggaggt gatgaaagaa
    10681 cccgggtgcg agcagttcga ggtgtttcag ag
//
LOCUS       DASSWZ010000197         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_459803, whole genome shotgun sequence.
ACCESSION   DASSWZ010000197 DASSWZ010000000
VERSION     DASSWZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459803"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..451)
                     /locus_tag="VFN58_04825"
     CDS             complement(35..451)
                     /locus_tag="VFN58_04825"
                     /inference="COORDINATES: protein motif:HMM:NF017482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HET8917347.1"
                     /translation="MAKATPIRRDHHLRLLEYPLAGGWKALVGRTDADNDYLSLTVAR
                     PDDWWFHVRGVPGGHVILQGPPGEEPDRETLKRAAAIAAYHSKARRAGLVAVSCTRAR
                     YITKPRHAKMGTVQIRKEVVLKVRPAAGEELAKHGK"
     gene            complement(569..1009)
                     /locus_tag="VFN58_04830"
     CDS             complement(569..1009)
                     /locus_tag="VFN58_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET8917348.1"
                     /translation="MELVRWEPFESLNKMHARINDLFGESFGRSWEKPSSAMWYPPVD
                     VLESKDAYLIRAELPGMKKEDFHLEVKDGTLTLTGERQSEKATDGVEYRTVERVNGKF
                     WRSFSLPETVKHDGIQATYKDGILEIRVPKAEEAKPRQIEISVH"
     gene            complement(1255..1824)
                     /locus_tag="VFN58_04835"
     CDS             complement(1255..1824)
                     /locus_tag="VFN58_04835"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET8917349.1"
                     /translation="MLILRTSIGRFEPSAQMGRVRPVNFLAMSDKSKYTLAQQNLTHT
                     RQTAAARRREAMSDPVLRGSCLCKAVRYEVRPPFIRFAHCYCSRCRKATGGVRATNIA
                     VVPDQFRWVSGEELISRYDLPEARSFARQICKRCNCPVPHKSRGGEQVIVPAGTLDDD
                     PGSKPSGHRNWGSRVGWATVDESHLPVSE"
     gene            complement(1824..3008)
                     /locus_tag="VFN58_04840"
     CDS             complement(1824..3008)
                     /locus_tag="VFN58_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET8917350.1"
                     /translation="MSYASEDRNQAEAIYLSLRGQGHTVFFDRTDLPPGEEYDARIRR
                     GIERSQLLVFLVSPYSLDAGSYTLTELAIAQKTWEHPTGKVLPVVVRPTLLDRIPPYL
                     KSVTLLETEGNVVATVADAVHRIALEQRRAILRTVAKGLAIAGIVCAGGYFYWAHRQP
                     SRETTGKDGAPALAVPAGNFTMGDDEQSPLREIYLDAFYIDKYEVTVARYAKFLQTTG
                     GVRAPDLWEETSLAGANEMPVVGVDWHDADAYCKWAGRRLPTEAEWEKAARGTDGRTY
                     PWGNDEPTSLRANFGKSSESPYKGGLAPAGRHEAGSSPYGVQDLAGNAAEWVADWFAE
                     GFTRGDVRNPKGPEGGKGRVIRGGGWYDPPDRLKSSWRMHANPGHRGDDVGFRCAQDF
                     SR"
BASE COUNT          626 a          942 c          788 g          720 t
ORIGIN      
        1 ttaagatttc tccactcttt tgtctttacc cgattcattt cccatgtttt gccaattctt
       61 cgccggccgc cggtcggacc ttgagcacaa cttccttgcg aatttgcacg gtacccattt
      121 tcgcgtgtcg aggcttggtg atataacgag ctcgagtaca tgagaccgcg accaagccag
      181 cgcgtcgggc tttgctgtga tacgcggcga tagcggctgc acgctttaac gtctcgcgat
      241 cgggctcctc tcctggtggc ccctgcagta taacatggcc cccaggaaca ccacgcacgt
      301 gaaaccacca atcgtccggt cgagccaccg tcaagctcag gtaatcgttg tcagcatcgg
      361 tgcgaccgac taacgctttc cagccgcccg ccagcggata ctcgagcagt cgcaaatggt
      421 ggtcgcgccg tatcggcgtc gctttagcca cagaaagtat aatccacgtg atcttctgca
      481 aaccttaata caacgcactg gcaatgaaaa ataaaaaaag gggaagcagt ttgcttcccc
      541 tttttgttgt atttgtgaac gtctgagatt aatgcacgct gatttcgatt tgccgcggct
      601 tagcctcttc ggctttcggg acgcgtatct ccaaaatgcc gtctttgtac gtggcttgga
      661 tgccgtcgtg ctttacggtt tccggaagcg aaaacgatcg ccagaatttt ccgttaaccc
      721 gctccacagt gcggtattcg acgccgtcag ttgccttctc ggattgtcgc tccccggtca
      781 gagtaagcgt tccatctttt acttccagat ggaaatcttc cttcttcatc cccggaagct
      841 cggcgcgaat caggtacgcg tctttgcttt ccagaacgtc taccggtgga taccacatgg
      901 cagaactggg tttttcccaa gagcggccaa agctctcccc gaataagtcg ttaatccgcg
      961 cgtgcatctt gttcaaactc tcaaacggtt cccatcttac taattccata atcaggcctc
     1021 cttttcgacc ttgcgtattt gctgtttgaa agttaagcat gaccctgaaa gctgtcaact
     1081 cacttgacag accgcctgtg cgtgcttaaa tttcatcagt taaagatctc aaaagagcaa
     1141 tcttctattc cgatccttgc agcaacaacc gttcgttatg cttcgacaca cgtagggcat
     1201 tctcagggca ggagcagctg aattcgtcag tttctctaag tgctctctta ccggctactc
     1261 ggaaactggc agatgggact cgtctaccgt ggcccaaccg acccggctcc cccaattccg
     1321 atgacccgac ggtttactcc caggatcatc gtccagagtg cctgcgggaa caatgacctg
     1381 ctcgccgccg cgtgatttgt gaggcacggg gcaattgcaa cgcttgcaga tctgccttgc
     1441 aaagcttctt gcttccggca gatcgtagcg cgaaattagt tcttctccgg acacccagcg
     1501 aaactgatcg ggaaccaccg cgatattcgt cgctcgcact ccacctgtcg ccttccggca
     1561 acgggagcag taacagtgag cgaatctgat gaacggcggc ctcacctcat atcgcaccgc
     1621 tttgcaaaga caacttcctc ggagcactgg atcggacatc gcttccctcc ttctcgccgc
     1681 agcagtttgt cgtgtatggg tcaggttttg ttgggccaga gtgtatttgg atttatcgct
     1741 catcgcaagg aagttcacag gtcttacgcg acccatttgt gccgacggtt cgaagcgacc
     1801 gatggacgtt cgcaggatca gcattaccgg ctaaagtcct gggcgcatcg gaatccgacg
     1861 tcgtccccgc ggtgacctgg attggcgtgc atgcgccagc tcgattttag tctatccgga
     1921 gggtcgtacc atcctccgcc tcgaatcacc ctcccttttc cgccctcggg tcccttcggg
     1981 ttcctgacgt cgccgcgggt gaagccttcg gcgaaccaat ccgctaccca ctccgcagca
     2041 ttgccggcaa gatcctgcac accgtacgga ctgctaccgg cctcgtgacg acccgccggc
     2101 gcgagccctc ctttgtaagg actctcagag gacttaccga agttcgcacg cagagaggtc
     2161 ggctcgtcat taccccaagg atatgtccgt ccatccgtgc cacgcgctgc tttttcccat
     2221 tccgcctctg taggcaagcg ccggccagcc cacttgcagt atgcatcggc gtcgtgccaa
     2281 tccaccccga ccacaggcat ctcattggcc ccggcgaggc tcgtctcctc ccaaagatcc
     2341 ggggctctca cgccgccggt tgtttgcaaa aatttagcat agcgcgccac cgtcacctcg
     2401 tacttgtcga tatagaaagc atctagataa atctcccgca gcggcgactg ctcgtcgtct
     2461 cccatcgtga agttaccggc gggtacggcc agtgcaggcg ccccatcctt gccggtggtt
     2521 tcccgagacg gttggcgatg cgcccaataa aaatacccgc cggcgcacac gatacctgcg
     2581 attgccagcc ctttggcgac ggtcctcagg atcgcacgac gttgctccag ggcaatgcgg
     2641 tgcacggcat cggccaccgt ggccaccaca ttcccttcgg tttcgagcag ggtaaccgac
     2701 ttcaggtaag gcggaatgcg gtccaatagg gtcgggcgaa cgacgaccgg cagaaccttt
     2761 ccggtgggat gttcccaagt cttctgagcg atggcgagtt cggtgagggt gtaactcccg
     2821 gcatccaggg agtacgggct gacgagaaag actagcagct gggatctctc gatgccgcgc
     2881 cggatccgtg catcgtattc ctctcccggc ggcaggtccg tccggtcgaa gaataccgtg
     2941 tgcccctggc cgcgcaaaga caggtagatg gcttccgctt ggttgcgatc ctcggaagca
     3001 taactcaaga acactctcat catccggatg aaagcacgca gacgaaattc ttacaatcgc
     3061 cgggagactc tgttgg
//
LOCUS       DASSWZ010000198         7356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1393584, whole genome shotgun sequence.
ACCESSION   DASSWZ010000198 DASSWZ010000000
VERSION     DASSWZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393584"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFN58_04845"
     CDS             <1..235
                     /locus_tag="VFN58_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917351.1"
                     /translation="TIKVLLTVGENDKGHWVKGDRLQDKREMFMGAKYAATTRGAHVV
                     LIPRYGHVGYAELHNEKIAYLWLWAVKSGYFDG"
     gene            548..1636
                     /locus_tag="VFN58_04850"
     CDS             548..1636
                     /locus_tag="VFN58_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917352.1"
                     /translation="MIAQTIGRFALPVALIAATLVATVVASFGAQSKPLTAADLAFYQ
                     GTDREKILLEGAKKEGQVTFYTSNTWVAGPVSQEFEKKYPFIKANVWRSDSKALLKRL
                     TEELTAGRSIADVVETSPEYMSIMLREKLLQEHFSPELSAYDDEAKIKGKSGVYGWTN
                     REIYISLGFNSKLIPPTEAPKSIKDYLDPKWKGKMSIAGTTTGVQWVGALLEMMGREF
                     LDKLSAQEINIQNISGAALSGLVASGEVPLSPTIFNSNIFTHKQKGAPVEWRPLDPVI
                     AGVGTSGMVLNAPRPHAALLFLDYLHSKEGQQVAMKGGLSSARTDIGSLEQKFTKVYI
                     ESKYTPEEFEKKIDEWEGLMRKLFLRKR"
     gene            1785..2729
                     /locus_tag="VFN58_04855"
     CDS             1785..2729
                     /locus_tag="VFN58_04855"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET8917353.1"
                     /translation="MAHTVLITGANGFIGSHTTVLMESLGHRVVAVDAVPRSTDLSLL
                     GIKTPSHIIDVTDAAAFRELSAKEKPTHIFHAAHPPRNEDPSVLNFCYHAMTNILEAA
                     KALKMRRVVYSSSASIYGQLKKPDHSLVKEEDAVTIYPTYFYRAAKTVSEWMGDFYKE
                     KHGVSFVALRYSSVYGPGLYRSIPLELKKGILGQRCRPFLTRPLDDLIYVDDVVDAIR
                     RALFSDKPLSRAYNIGLDKAYTDEDLTQAIRKALPDLKFEIGQHPNAAEVGPHRNRDP
                     LDISRANEELGWTPKIYLEEGIARLAAWLNKHKAQLRP"
     gene            2759..3838
                     /locus_tag="VFN58_04860"
     CDS             2759..3838
                     /locus_tag="VFN58_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917354.1"
                     /translation="MHLTRPLRTILILASAAFYCFFTWTAFAASAAMPISQMALYQGA
                     DREKILIEGAKREGQFTLYTSHTWFRTLVKDFEKKYPFVKTSEWRNDSKNVIRKVLEE
                     VKSGRILVDVVETTADGMGVMKREGLFQEYYSPEARYYPNELKPRGKNGFFYLPDRET
                     YNSLGFNTTLVPTAAAPRNFKDLVDPKWKGKMAITSTTTGVRWIGNALESMGREYLDK
                     MAEQEVSVQDMAPAALINLVASGEVPLSPTIFDANVTIAKQKGAPVEWRPLEPVVTTV
                     GSSALMAKAPNPHSALLFIDFLLSKEGQQLIMKGGLWSPREDTGSLEQKFKKTYLDEK
                     YSLEELEVKFAQWENLMRQLFIRKR"
     gene            3875..4954
                     /locus_tag="VFN58_04865"
     CDS             3875..4954
                     /locus_tag="VFN58_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917355.1"
                     /translation="MPSIRFHRIGSIPVVLLSYLLSAVDALPASAPTTVSQLALYQGA
                     DREKILIEGAKKEGQFTIYDSHTWFRTIAKEFEKKYPFVKVSEFRTDGRNLIKRALEE
                     IAAGQYIADVIATTSEQMAIMKREGVFQEYALADARYYPDDVKMKGKNGFYYIGHYET
                     YNSLGFNTALIPPAEAPKTMSDLLNPKWKGKMSIVSTTTGTRWIGSTLNVMGREFLDK
                     MADQDVKVQNMSGAALAGLVVSGEVPLSPTIFDANIHTAKQKGAPVEWRPLEPVVTTV
                     SYSGLTLKPTHPHAALLFLEYLHSKEGQQLIMKGGLWSPREDIGTVEQKFKKSYIDEK
                     YSIDEAEKKFAEWDKLMQQLFIRKR"
     gene            5021..6094
                     /locus_tag="VFN58_04870"
     CDS             5021..6094
                     /locus_tag="VFN58_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917356.1"
                     /translation="MVRLHRAPFTPVAFAFYLLTACEVFAASASMTVPQLALYQGADR
                     EKILIEGAKREGQLTLYDSHTWFRTYVKDFEKKYPFIKVSEWRSDSKNLLSRAMTEFN
                     SNRSLVDVIETTAEAMWVMKRDGMFQEYFTPEARHYPDDVKAKGKNGLYYLGDRETYN
                     SLGFNTTLIALHEAPKSLKDLLDSKWKGKMSIAGTSTGVRWVGSTLDTLGREFLNKMA
                     GQEVTVQNMSGAALAGLVASGEVPLSPTIFDADVTVAKQKGAPIEWRPLDPVVTTVGY
                     SGLSSKAPYPHAALLFLDYVHSKEGQQLMMKGGLWSPRNDLGSLDQKFKKIYLDSKYS
                     LDELEKKLTEWENLMIQLFVRKK"
     gene            complement(6161..7009)
                     /locus_tag="VFN58_04875"
     CDS             complement(6161..7009)
                     /locus_tag="VFN58_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8917357.1"
                     /translation="MAKQPFKWPNNARIAFVLGIAFEAWDRNKPSRGGALQSPLPANA
                     LCKRDYSTETFREFGGKIGVRRLLDICDKYDLKVSMPINGLTCEYWPDLIKYAHGRGH
                     EMVAHGWDQGEYLFMLTREQERENIFKTVEAIKKLTGERPTGWSSPGVRSTDNTLELN
                     LEAGLLYNCDFHDDEMPYPIKIGDKMLIMIPHQYTINDHRMQEEGTRHDFFEKFKDEF
                     DYRYKIGPENPTMMNVITHGYLSSRVPAIDTFEQIIKYAKGHKDVWFARRGDIAAWAR
                     KYYEGE"
     gene            7103..>7356
                     /locus_tag="VFN58_04880"
     CDS             7103..>7356
                     /locus_tag="VFN58_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET8917358.1"
                     /translation="MKPRALAYPDLILFNGKIRSFAADSSIHEALACAGGRIVATGKS
                     EDIRRLAGPDTQSIDLQGRTSIPGLTDTHVHLSEKGTAEM"
BASE COUNT         1855 a         1995 c         1919 g         1587 t
ORIGIN      
        1 aaccatcaaa gttcttctca ccgtgggtga gaacgacaaa ggtcactggg tgaaaggcga
       61 tcggttgcag gataagcggg aaatgttcat gggcgctaag tacgcggcta ctactcgggg
      121 cgcgcatgta gttttgattc cccgttacgg ccatgtgggc tatgccgaat tgcacaacga
      181 aaaaattgct tacctatggc tatgggcggt taagtcaggc tactttgacg gctaactttg
      241 gttgctcatt ctggcggaaa gaaagacaga ataacgccat ggccaaaaaa atcatcgacc
      301 agcttgtcga ctatatattg gctgcacccg acgcttcgtt acctagcgag gtcgttcagc
      361 catgcaagag gcggtggagg caatgcatcg tctaatcgaa cggcacaaga ttagtcgccg
      421 atgtcgtcaa ggttctcatc cctttgtcgg aaagagctgc ccggaagcgc gcgcgctcat
      481 tggccctcgg aatgaaacgt ttaggaacta ttctgataag tgtctcttcg cgaaggagga
      541 aatataaatg attgcacaaa cgatcggcag atttgcgctt ccggttgcac tcatcgccgc
      601 aacgcttgtg gctactgtcg tcgcgtcctt tggagctcag agcaagcccc tgaccgcagc
      661 tgaccttgcc ttttaccaag gaaccgatcg cgagaagatc ctcctggaag gcgcaaaaaa
      721 agaagggcaa gtgaccttct atacgtcgaa cacctgggtc gccggtccgg tctctcaaga
      781 gtttgaaaag aagtatccgt ttatcaaggc gaacgtctgg cgcagcgact ccaaggcact
      841 gttgaagcgg ttaactgaag aattaaccgc cggccgctcc atcgccgatg tcgtcgaaac
      901 cagtcctgaa tacatgtcga tcatgttgcg cgaaaagctg ctccaagagc acttctcccc
      961 ggagctcagc gcctatgacg acgaagcaaa gataaaaggc aaaagcggtg tatacggttg
     1021 gaccaaccgg gagatttaca tcagcctcgg ctttaactcg aagctcatcc ctccaactga
     1081 agcacccaaa agcatcaagg actacctcga tcccaaatgg aaagggaaaa tgtccatcgc
     1141 cggaacaacc accggggttc aatgggtggg cgcccttctt gaaatgatgg ggcgtgagtt
     1201 tttggacaag ctgagtgcgc aggaaatcaa cattcaaaat atttcagggg cagctttgtc
     1261 aggactggtc gcgtccggag aggtgcccct ttccccgacg atcttcaatt cgaatatctt
     1321 cacccacaag caaaaaggcg cgccggtaga atggcgcccg cttgatccgg tgatcgccgg
     1381 cgtcgggacg tcagggatgg tactcaatgc gcctcgtcct cacgctgcgc tgctgtttct
     1441 cgactatctc cattccaagg agggacaaca ggtcgccatg aagggtggcc tcagctcggc
     1501 gcgcaccgac atcggttctc tcgagcaaaa gttcacgaag gtctacatcg aaagcaaata
     1561 cacgccggaa gaattcgaga aaaaaatcga cgaatgggaa ggcctaatgc gcaaactctt
     1621 cctacggaag cgctagtgtg ggcggggttc aaacccgccc acactggatt cccttcccgt
     1681 tttcacggaa atgacgatcg gcgggtcaac caatttattg gtccgttctt gacgcaggag
     1741 acgggcgaaa cgaccgctat ctcatcgagg aggcaatcga aagtatggct cacacagttt
     1801 tgatcacggg cgccaacggc tttatcggct cccatacaac agtattaatg gaatcacttg
     1861 gtcacagggt cgttgccgtc gacgccgtac cacggtcgac ggatctctcc ttgctaggga
     1921 tcaaaactcc gagccacatt atcgatgtca cggatgccgc ggctttccgg gaactctctg
     1981 ccaaggaaaa gccgacgcat atctttcacg ccgctcaccc acctcggaac gaagatccct
     2041 ccgtgctcaa cttttgctac catgcgatga ccaacatcct tgaggcggca aaggcgctca
     2101 agatgcggcg ggttgtctat tcaagctccg cttcgattta cggccaactg aaaaaacctg
     2161 accatagtct ggtcaaagaa gaagacgcgg tcacgattta tccgacctac ttctaccgcg
     2221 cggcgaaaac cgtgtcggaa tggatgggcg atttttataa ggagaagcac ggcgtaagct
     2281 ttgtcgcgct cagatactcc tcggtttacg gcccaggtct ataccgaagt attccgctgg
     2341 agctcaagaa aggcatcctc ggccagcgct gccggccgtt tctcactcgc ccgctcgacg
     2401 atctgatcta tgtggacgac gtcgtcgatg cgattcgccg cgcgctcttt tcagacaaac
     2461 cgctcagccg cgcctacaac atcggcctcg acaaggccta taccgacgag gatctcacac
     2521 aggccattcg aaaagcgcta ccggatctga aattcgaaat cggccagcat cccaacgctg
     2581 ccgaggtcgg cccccatcgc aatcgtgacc cgctggacat ttcccgcgcc aacgaagagc
     2641 taggctggac tcccaagatt tatctcgaag aaggcatcgc tcgcctcgcc gcttggctga
     2701 ataagcacaa ggcccaactg cgcccatagc cagtgtctat cagatgagga ggacgtcaat
     2761 gcacttaacg cgacccctac ggaccatctt gattctcgcg agtgcggctt tctattgctt
     2821 cttcacttgg acggcgtttg ctgcgagtgc tgccatgccg atttcgcaaa tggcactcta
     2881 ccaaggtgcc gaccgagaaa aaattctaat cgaaggagca aaacgagagg gccaattcac
     2941 tttatacaca tcgcatacgt ggtttcgcac tctcgtgaag gacttcgaga aaaaatatcc
     3001 gttcgtcaaa acttccgagt ggcgcaacga ctccaagaac gttatccgaa aagtgctcga
     3061 ggaagtaaaa tccggccgaa tcctggtcga cgtcgtggaa acgaccgccg acggcatggg
     3121 ggtcatgaaa cgggaaggcc tattccagga gtactattct cccgaagcac gttactaccc
     3181 caatgagctc aagccgagag gaaaaaacgg gttcttctac ctgccggacc gggagactta
     3241 caatagcctt ggattcaaca ccacactcgt tccaaccgcc gcggccccgc gaaacttcaa
     3301 agatttagtc gatccgaaat ggaagggcaa gatggcgatc accagtacaa ccaccggagt
     3361 gcgctggatc ggtaatgcat tggagtctat gggacgcgag tatttggata agatggccga
     3421 acaggaggtg agcgtccagg atatggcgcc tgcggcgtta ataaatttgg tcgcctccgg
     3481 ggaggttcca ttgtcgccga cgatcttcga tgccaacgtc actatcgcaa agcaaaaggg
     3541 agcgccagtc gaatggcgtc ctttagaacc cgtggtcaca accgtgggtt cctccgcgct
     3601 catggctaaa gcgcccaatc cccacagcgc gcttctgttt atcgatttcc tcctttccaa
     3661 ggaaggacaa cagctgatca tgaaaggggg actctggtcg ccgcgcgagg ataccggcag
     3721 cctggagcag aaattcaaaa aaacctacct tgacgagaag tattcgttag aggaattgga
     3781 ggtaaaattt gcccaatggg aaaatctcat gcggcaactc tttattcgaa agcggtaggt
     3841 cacatcgata tgagtcgcgt aagggagaac gacaatgcct agcattcgat tccaccggat
     3901 tggatcaatc cccgtggttc tattgtcata cctgctcagt gcggttgatg ccctccccgc
     3961 ttcagcgccc acgacagttt ctcaattagc cctctaccaa ggggccgacc gcgagaagat
     4021 cctcatcgaa ggtgccaaaa aagaaggcca gtttacaatc tacgactccc atacctggtt
     4081 tcgaaccatc gccaaggagt tcgagaagaa gtacccgttt gtcaaggttt ctgagtttcg
     4141 taccgacggc aggaacctga tcaagcgcgc gcttgaggag atcgcggcag gccagtatat
     4201 cgcggacgtc atcgcgacga catccgagca gatggcgata atgaagcggg agggcgtgtt
     4261 tcaagaatac gccttggcag acgcgcgcta ttacccggat gacgtcaaaa tgaaaggaaa
     4321 aaacgggttc tactatatcg gacactacga aacctataac agtttggggt tcaacactgc
     4381 gctgatccct cccgcggaag cgccgaaaac catgagcgat ctcttgaacc cgaaatggaa
     4441 agggaaaatg tccatcgtca gcaccacgac aggcacccga tggatcggca gcaccttgaa
     4501 tgttatggga cgcgagttcc ttgacaagat ggcggatcaa gacgtgaagg tgcaaaacat
     4561 gtcgggcgcg gctttggcgg gcttggtcgt ctcgggcgag gtgccgctat cacctaccat
     4621 cttcgatgct aatattcata cggcaaaaca gaaaggggcg ccggtggaat ggcgcccgtt
     4681 agagccagtc gtgaccacag tcagctattc cggattgact ctgaaaccaa cccatcctca
     4741 cgccgctctt cttttcctcg aatatctcca ttccaaagaa ggacaacaac taataatgaa
     4801 gggtggtctc tggtcgccgc gagaagacat cggaacagtg gagcagaagt ttaagaagtc
     4861 ctacatcgac gaaaagtact caatcgatga agcggagaag aagttcgccg aatgggataa
     4921 gctgatgcag cagctcttta tcaggaaaag gtaaggtagg tcaggaagaa atgactctga
     4981 cgacgaattg accccaatgc atccaaagcg acgatatacg atggttcgac tccatagagc
     5041 tccttttacc ccagtcgcgt ttgcgttcta tctattgact gcctgtgaag tcttcgccgc
     5101 cagcgcgtcg atgacagttc cgcagctggc cctttatcag ggagcagacc gggaaaagat
     5161 cctcatcgaa ggcgcaaagc gggaaggcca gctgactttg tacgattcgc acacctggtt
     5221 tcgtacctac gtcaaggatt tcgagaaaaa gtacccgttc attaaggttt ccgaatggcg
     5281 cagtgactcc aagaacttgc tcagtcgagc catgacggag ttcaattcca accgctcctt
     5341 ggttgatgtc attgaaacca cggcggaagc catgtgggta atgaaacgag acgggatgtt
     5401 tcaggaatat tttactccgg aagcgcgcca ctatcccgac gacgtcaaag ccaaaggaaa
     5461 gaacggactc tactatctcg gcgatcgcga aacttacaat agcttgggct ttaatacaac
     5521 gctgatcgct ctgcacgaag cgcctaagtc gctcaaggat ctgctggatt cgaaatggaa
     5581 agggaaaatg tccatcgccg gcacgtccac gggcgtgcgc tgggtcggta gcaccttgga
     5641 taccctcggt cgtgagttcc tgaacaaaat ggccggacaa gaggtgaccg tgcaaaatat
     5701 gtccggcgcc gctttggcag gcttggtagc atcgggcgag gtgcccctat cgccaacgat
     5761 tttcgacgcc gatgttacag ttgcaaagca aaaaggcgcg ccgatcgaat ggcgtcctct
     5821 agacccggtc gtgaccacgg tgggctattc cggattgtca tccaaagcac cgtatcccca
     5881 tgcggctcta ctttttcttg actatgtcca ttcgaaggaa ggacagcagt tgatgatgaa
     5941 aggcgggctc tggtcgccgc ggaacgattt gggatccttg gatcagaagt tcaagaaaat
     6001 ctacctcgac agcaaatact ccctcgatga gctcgaaaaa aaactcaccg agtgggaaaa
     6061 tcttatgatc caacttttcg tcagaaagaa ataaatgctc cttctaaaac gacttgatta
     6121 ccttgagtct gcctgaccta cttactcccg gtcacaacca ttactctccc tcatagtact
     6181 ttctcgccca ggccgcgata tccccgcgcc gggcgaacca gacatctttg tggcccttgg
     6241 cgtacttgat gatctgctcg aaagtgtcga tggccgggac gcgtgagctc aagtagccgt
     6301 gcgtgatcac gttcatcata gtggggtttt ccggtccaat cttgtagcgg taatcaaact
     6361 cgtctttgaa cttctcaaaa aaatcgtggc gcgtcccttc ctcttgcatc cggtgatcgt
     6421 tgatcgtgta ctggtgcggg atcatgatca gcatcttatc gccaatcttg atcggatagg
     6481 gcatttcgtc gtcgtggaag tcgcagttgt acagcagccc cgcttcgaga ttcagctcca
     6541 gcgtattgtc cgtagaacga accccgggcg acgaccaacc tgtcggacgt tcgccggtga
     6601 gcttcttaat cgcttcgacg gtcttgaaaa tgttctctcg ctcctgctcc cgcgtcagca
     6661 tgaaaagata ttcgccctgg tcccagccat gggcgaccat ttcgtggccg cgaccgtggg
     6721 cgtacttgat gagatccggc caatactcac aggtcaaccc attgatcggc atgctgactt
     6781 tcaggtcata cttgtcgcaa atatcgagca gccgtctgac accgatcttc cccccgaatt
     6841 cacgaaaggt ttcagtggaa taatcacgct tgcaaagcgc gttcgccggg agcggcgatt
     6901 gcaacgcacc gccccgcgac ggcttatttc tgtcccaggc ttcaaaggcg atgccgagca
     6961 cgaaagcgat cctcgcgttg ttgggccact tgaaaggctg tttagccata tcgcattcct
     7021 ccaaatttgc agactgttga cttctagtaa cgaagcgact tatagccaaa gagtctgaaa
     7081 tgagcaagga ggaaaccttt tcatgaaacc gagggccctc gcctatcctg atttaatact
     7141 attcaatggc aaaatccgca gcttcgccgc ggatagttcg atccatgagg cactggcgtg
     7201 cgccggcggg cgcatcgttg caaccggcaa atccgaggac atccgccgcc tagctggccc
     7261 tgacactcaa tccatcgatc tccaggggag aacaagcatc cctgggctta ccgataccca
     7321 cgttcatctg tccgaaaaag gcacggcgga gatgga
//
LOCUS       DASSWZ010000199         5861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_304559, whole genome shotgun sequence.
ACCESSION   DASSWZ010000199 DASSWZ010000000
VERSION     DASSWZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304559"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="VFN58_04885"
     CDS             complement(<1..1736)
                     /locus_tag="VFN58_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8917359.1"
                     /translation="MSADGSKQADCEYIVVGSGAGGGPVAANLAEAGHKVLLLEAGGD
                     PMQLADNRLPDDYNVPAFHALASENEALKWDFFVRHYADDGYQARDPKFLCERDGVLY
                     PRAGTLGGCTAHNALFMVYPHNADWDQIAGLTQDSSWNAKSMRKYFQRLEDCHHRPVQ
                     RWLYKSLRLNLSRHGFKGWLSTEKAIPRAAFRDLKLVKTIVGSAKESFEESPGLIERV
                     RWFMESLGDPNDWGLVKKNAFGIRYTPLSTRGHCRIGSRERVLEVAQKYPNRIKVELN
                     ALVTSVILDHENRAIGVNYLKGERLYRAHANPSSEPGEQHQAFASREILLSGGAFNTP
                     QLLMLSGIGPRHELKRHGIDVRVDLPGVGTNLQDRYEVSVVNRMKDDWEALRGAQFHK
                     GDSQYQQWVTEKKGVYITNGGVLVVIKKSKPSRPLPDLFCIGLLGKFEGYYPGYSKLT
                     EQYHNYLSWVVLKAHTSNTAGTVTLRSADPRDTPLINFRYFDEKCDPDLEDLDSVVEG
                     IKFVRSLTGGMSEHIAEEELPGKNLKSRDELRRFVKENAWGHHASCSCAIGPRGNGGV
                     VDSKFRVYGTKGL"
     gene            complement(1795..2868)
                     /locus_tag="VFN58_04890"
     CDS             complement(1795..2868)
                     /locus_tag="VFN58_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917360.1"
                     /translation="MEPDYFGTEQVTEEDRAYNGSRFSAVRDALFANSYQKVWGRAGE
                     PPLPTYEVTLLNVLRGIFPFGQPYLFRQATERAVDSHADLRWGPDGRGFRRIVHPNGV
                     CLTGVWEITEQTDYSGYFAKGSRALVVGRYSSCCTETRRGHTRSLALVGKLFPTTDPK
                     HSEPLRTASFITQQDLAGDNTDYINDVELRNAPDTHAWRRGNGMPIFLVTGALFLRVD
                     KEPSIRQLYQIAELGKSKEQVTRTPEFMRLLVAPDQPRIKGAELDFRDEILAQIFDRG
                     DPTPKRTLTLYIEVTDEGSTRGTAAFQRRTFKNWRHIGKLTFDNAVASYNGDFVVHFN
                     HPTWRANRNDPSTATRISGRKVR"
     gene            complement(3032..5476)
                     /locus_tag="VFN58_04895"
     CDS             complement(3032..5476)
                     /locus_tag="VFN58_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917361.1"
                     /translation="MRNQKLIIAFVICTSVLVLISCGERKSVPGTVLDEARRAERMAS
                     SFPAADENYFKDMDGGIELTTNEVKGRNNWIVWTGGNDRFWDHLVNRSFGAVDFLKLL
                     SSHPNVKHLSRDTRWKNLGLVNEPCFEKAKGPRGDRFGLWLDTRSKDCPPDPFENEQK
                     YPGVKIGARGKNMPVGSYYGYATGIVGLRLFPNPDFDEAVTKKWDPERFYTDPKYYED
                     KNLVRPYRVGMSCAFCHVGPSPVKPPEDPENPKWENLNSNPGTQYFWVDRILFWDWEK
                     NDSSFVYQLLHTSLPGTLDTSFISTDYINNPRTMNAIYSVGPRLGPALRWGQEKLAGG
                     SLKNKQFQDFSQTAALSQFYKAPDTVWTPRVLKDGSDSVGLLGALNRVFINIGLFSEE
                     WLLHFNVFVGGKKITPIRIEDAEKNSVYWNATVAQTADVALFFLRAAKPDLLKDAPGG
                     GQYLTQDKARLNRGKVAFAERCARCHSSKIPEAPTGVDESQWAQYWAWTKTDDFKQKM
                     TAMVMADDFLNDNYLSTERRIPVTLLQTNACSPLATNALAGNIWDNFSSQTYQTLPSV
                     GKMTLHNPIDGTTFDYELPGGGRGFTRPPSLISLWSTAPFLLNNSVGKFRWEASVEAR
                     MDSFNDSIEQMLWPEKRKKDADVIKALGLPDSKALKVPGFIYRTTARSYLRAPFGYLP
                     DGLEKLLSWGEWFQHLFPWLFGDGMIEIGPIPKGTPVNLLTNLDLEQNKVELARVLLK
                     MKRALKKVEGANDEEVAKVFTDPELGLVPDLLKLSKCPDFVVNKGHYFGTDLFKEEPP
                     LSDDDKRALIEYLKTF"
BASE COUNT         1273 a         1684 c         1543 g         1361 t
ORIGIN      
        1 agccccttcg tgccataaac tcgaaacttg ctgtcgacca cgccgccatt tcctcgcggt
       61 cctatcgcac aactgcatga ggcgtggtga ccccatgcat tctccttgac gaaccgccgc
      121 aactcatcgc gactcttaag attttttccc ggtagctctt cctccgcgat gtgctcgctc
      181 atcccgccgg tcagcgagcg cacaaacttg attccctcca cgacagagtc aagatcctcc
      241 agatcgggat cgcatttttc atcgaaataa cggaagttga tcaggggtgt gtcgcgagga
      301 tcggcggatc tgagtgtcac agttccggca gtgttggatg tatgggcctt caagacgacc
      361 cagctcaggt aattgtggta ttgctcggtg agtttcgaat agccgggata atagccctcg
      421 aacttaccca gtaggccgat gcagaaaagg tcgggaagcg gccgactcgg cttcgatttc
      481 tttataacca cgagcactcc accattcgtg atgtaaacac ctttcttctc cgtcacccac
      541 tgttgatact ggctgtcccc cttatggaat tgcgcacccc gcagcgcctc ccaatcgtcc
      601 ttcatgcgat tgactacgct gacctcatag cgatcctgaa gattcgtacc gacacccggg
      661 agatccactc gcacgtcgat gccatgccgc ttcaattcgt gccgcgggcc gatacctgaa
      721 agcatgagca gctgtggggt gttgaaagct ccgccggaca agaggatctc gcgagaagca
      781 aacgcctgat gttgctcgcc aggctcgctg ctcggattgg cgtgcgcccg gtacagccgc
      841 tcgcctttga gatagttcac accgattgcc cggttctcgt gatcaagaat cacagaggtg
      901 accagcgcat tgagctcgac ctttattcta ttgggatatt tttgcgccac ctccaagacc
      961 cgttcccgac tgccgatgcg acagtggccg cgtgtgctta ggggggtata ccgtatgccg
     1021 aaggcatttt ttttcactag cccccaatcg ttgggatcgc ccagggattc cataaaccac
     1081 cgcactcgct cgatcagacc tgggctttcc tcgaaggatt ccttcgccga tccaacgata
     1141 gtcttcacca acttgaggtc tcggaaagcc gccctgggaa tcgccttttc ggtggagagc
     1201 caacctttga atccgtggcg ggagagatta aggcgaagcg atttgtaaag ccagcgctga
     1261 accggtcgat ggtgacagtc ctccaatcgt tggaaatact tgcgcatgct cttggcgttc
     1321 caggaagagt cttgggtgag tccggcgatc tgatcccagt cagcgttgtg aggatagacc
     1381 atgaacagcg cgttgtgggc ggtgcagcct cccaaggtgc ctgccctggg atagagaacg
     1441 ccgtcacgct cgcatagaaa cttgggatca cgcgcttggt atccatcgtc cgcgtagtga
     1501 cgcacgaaaa aatcccactt tagtgcctcg ttctcggacg ctaaagcgtg aaaggccggc
     1561 acgttgtagt catcaggtaa ccggttatca gccagttgca tggggtcgcc gcccgcttcc
     1621 aggagcagaa ccttgtgacc tgcctccgcc agattcgccg ccacgggacc gccgccagcc
     1681 cctgagccga cgacgatgta ttcacaatca gcttgtttcg agccgtccgc actcatgcga
     1741 gcgcaccctc gtctgctttg cgatccctaa cccaccaggg caaacaccgg actgtcatcg
     1801 caccttgcgt ccactgatcc gggtggcagt tgacggatcg ttccgattcg cgcgccaggt
     1861 gggatggttg aaatggacca caaaatcgcc gttgtaagaa gcgacagcat tgtcgaaagt
     1921 cagttttcca atgtgacgcc aattcttgaa ggtgcgccgc tgaaaggctg ctgtgccccg
     1981 agttgacccc tcatcggtca cttcgatgta taaggtgaga gttcgtttcg gcgtcggatc
     2041 acccctatca aaaatctgcg ccaagatttc gtcgcgaaaa tccaactctg cccctttgat
     2101 cctgggctga tctggcgcga ccagcaggcg catgaactcc ggcgttcgcg tgacctgctc
     2161 ctttgacttg ccgagctcgg cgatctggta cagctggcga atcgagggtt ccttatcgac
     2221 ccggagaaag agggcgcccg tgacaaggaa aattggcatt ccattgccgc gacgccaagc
     2281 atgcgtatcg ggagcgttac gcaattccac gtcatttatg tagtccgtat tgtctccggc
     2341 caagtcctgt tgggtgatga agctggcggt acgtagcggc tcggagtgct tcgggtccgt
     2401 ggtaggaaac agcttgccta ccagcgccaa cgaacgcgtg tgaccgcgcc gagtctcagt
     2461 acaacagctg gaatagcgtc ccacgaccag cgcccggctt cccttggcaa aataaccgga
     2521 atagtcagtc tgttccgtga tctcccagac gccggtgagg cacaccccgt tcggatgaac
     2581 tatgcgccga aaccctctgc cgtccgggcc ccagcgcaaa tccgcgtggg aatctaccgc
     2641 tcgttcggtg gcctggcgaa acaggtaggg ctgaccaaat ggaaagatgc ctcgcagaac
     2701 gtttagcagg gtgacttcat acgtgggcaa tggtggctcg ccagcacgac cccacacttt
     2761 ttggtaggaa ttggcgaaca gggcatcgcg cactgcggag aagcgagatc cgttataggc
     2821 gcggtcttct tcggtaacct gttccgtccc aaaatagtcc ggttccataa cgcattccac
     2881 cccaagcacc accactatcc tccatcccgt tcggttagtc gcgctgcacc aggttcgcca
     2941 tcagctcctg atgccaccac aacatattca agcttcgctt gccggcgtcg cctgcccggc
     3001 ttatcgtctc tgactttccc tcaaccgcgc ctcaaaaagt tttcaggtac tcgataagtg
     3061 cccgcttgtc gtcgtcgctc aacggaggct cttctttgaa gagatccgta ccgaagtaat
     3121 ggcctttgtt gacgacaaag tcggggcatt tgctgagctt gagtagatcc ggcacgaggc
     3181 ccagctccgg atccgtgaat accttcgcca cctcctcgtc gttcgctcct tcaactttct
     3241 tcagcgctct tttcatcttg agcagtactc gagctaactc tactttgttt tgttcgaggt
     3301 cgaggttggt tagtaagtta accggagttc ccttggggat tggaccaatc tcgatcattc
     3361 cgtccccgaa aagccaggga aagaggtgtt ggaaccactc gccccaactc agcaacttct
     3421 ccagaccatc gggaaggtac ccaaacggag ctcttaagta gctccgcgcc gtggttctgt
     3481 agatgaagcc cggcactttg agcgctttcg agtcgggcaa accgagcgcc ttaatgacgt
     3541 cagcgtcctt ttttcgcttc tcgggccaga gcatttgctc gatcgagtcg ttgaaggagt
     3601 ccatccgtgc ttcgactgaa gcctcccacc tgaattttcc aaccgagttg ttgaggagaa
     3661 atggcgccgt tgaccacaaa ctgatcaatg acggcggacg agtgaagcca cggccgccgc
     3721 cgggcaactc gtagtcaaaa gttgttccat cgatcggatt atgcaacgtc atttttccga
     3781 cggagggcaa ggtctgatac gtctgcgatg agaagttatc ccaaatattt ccggcaagcg
     3841 cattggtcgc cagcgggctg cacgcattgg tctgcagcaa ggtaaccggt atgcggcgct
     3901 cagtggacaa gtagttgtcg ttgagaaaat cgtcggccat aaccatggcc gtcatctttt
     3961 gcttgaagtc gtcggttttc gtccaagccc agtactgagc ccactgggac tcatcaactc
     4021 ccgttggcgc ttcgggaatc ttgcttgaat ggcaccgagc acaccgctct gcaaaagcaa
     4081 ctttgccccg gttcaacctg gctttgtctt gagtcagata ctggccgccc cccggagcgt
     4141 ccttgagcag gtcgggcttt gccgccctta aaaagaacag cgccacatcc gccgtctggg
     4201 ccaccgtcgc attccagtaa acggaattct tttcagcgtc ctcgatcctg atcggtgtga
     4261 tcttcttgcc gccgacgaac acattgaagt gcagcagcca ctcttcgcta aacagtccga
     4321 tattgataaa aacgcgattg agcgcgccga gaagtccaac cgaatcggat ccatccttga
     4381 gcacgcgcgg cgtccatacg gtatccggcg ctttatagaa ctgtgagagc gcggcggtct
     4441 gcgagaagtc ttggaactgc ttgttcttga gactgccacc ggcgagtttc tcttgacccc
     4501 aacgcaacgc cgggccgagc cgcgggccga cgctatagat cgcgttcatc gtgcgcgggt
     4561 tgttgatgta atcggtggag ataaacgaag tatccaacgt gccgggcaga gatgtgtgca
     4621 acagttggta aacaaaactc gaatcattct tctcccaatc ccagaagagg atgcggtcca
     4681 cccagaaata ctgcgtgccc gggttggagt tgaggttttc ccacttggga ttctccgggt
     4741 cttctggcgg tttcaccgga ctcggaccga catgacagaa ggcgcacgac atgcccaccc
     4801 ggtacgggcg gactaagttt ttgtcttcat agtacttcgg atcggtgtag aatctttcgg
     4861 ggtcccattt cttcgttacc gcttcgtcga aatctgggtt cggaaacaga cgcaagccga
     4921 cgatgcccgt cgcgtaacca tagtaagatc ccaccggcat atttttgccc cgcgcaccga
     4981 ttttcacgcc gggatacttc tgctcgtttt caaagggatc gggcggacaa tctttactcc
     5041 gggtgtcgag ccaaagaccg aaacgatcac cacgcggtcc cttagctttt tcaaagcagg
     5101 gttcgttgac caggccgagg tttttccagc gagtgtcgcg gcttaagtgt ttaacattcg
     5161 gatgcgacga gagaagcttc aaaaaatcca ccgcaccgaa gcttcggttt acaagatgat
     5221 cccaaaaacg gtcgttgcca ccggtccaaa caatccagtt attgcgtccc ttgacctcat
     5281 tggtggtaag ttcgatgccg ccatccatgt ctttgaagta gttttcatct gcggccggaa
     5341 acgacgatgc catccgttct gcacgccgcg cctcgtcgag caccgtcccg ggaacggact
     5401 tcctttctcc gcaacttatc aggaccagca cactagtgca gataacaaat gcaatgataa
     5461 gtttctgatt cctcattcag tctccgtttc tgatcggatg ggaagaggcc ccgagaaaca
     5521 caatagcagc taccgaggcg tggttattcc tcgcatagga ttaatctctc tgtcaacaaa
     5581 attcttcgat tgctaatgga acgcaacgat gcgcaacaac gatggaactt ctattcgttg
     5641 ttgaaaccta cgaagggttc tgccagattc aaagtgatcc gaaacatgaa gacaaaaaca
     5701 gcgataattt cgattgataa cctgaaggcc gaagatctcc aggtgctcaa gcaagcgctg
     5761 cagagcgttc cgggggtgga tactgtcgat ttcaatctag aacggagcgt cgcggtggtc
     5821 gatttcgatc cgactcagtc tcacatcgat gactttttgc g
//
LOCUS       DASSWZ010000200         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1152963, whole genome shotgun sequence.
ACCESSION   DASSWZ010000200 DASSWZ010000000
VERSION     DASSWZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152963"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFN58_04900"
     CDS             <1..1047
                     /locus_tag="VFN58_04900"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET8917362.1"
                     /translation="QLSDIFEEFCATRLLDTLNSKKVERIRSLFDEKKVHILLQHDPD
                     PDALGSALALRELLGRNRITTPIVTFGEVTRPENLAMIHFLDIQIDRITYEDLHKDGA
                     QLALVDVQPPYFDQPLGRVDLVVDHHPKRTPFKARFTDLRTGYGATSTIFTEYLRAIG
                     MEPSQRLATALLYGIKTDTLFLERGSILADLSAFTFLYPFANKPMITRIERPALPRED
                     LEALGRALSRLQLDDGVAVIHMGEINREDVIPQMAEFCLHIEGVEWGVVSGLVKDRVV
                     ISVRNVGYVRSAGDIMKKLYDDIGSAGGHRAMAKAVIPLDRFKERYGEVSEKVIRQAI
                     VPMIVERDEQAAHG"
     gene            1040..1468
                     /locus_tag="VFN58_04905"
     CDS             1040..1468
                     /locus_tag="VFN58_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HET8917363.1"
                     /translation="MANDRGTYREIEHTADLGVEITAADLPALFASAGEALYALIADP
                     ATIENREAIKVSATGANPEDLLHTWLCELLALFNVEGFVGKRCEITHISDGQVQGRVS
                     GEKLDLKRHAFHTEIKGVTYHDFKVWQDAGTWHARVIFDV"
     gene            1513..2964
                     /locus_tag="VFN58_04910"
     CDS             1513..2964
                     /locus_tag="VFN58_04910"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET8917364.1"
                     /translation="MATEDIKLNRLEDYLWEIPRQGGMRVPGRIYASEKLMAGLKVDN
                     SLRQVMNVAHLPGIVGYSLAMPDIHWGYGFPIGGVAAISVDDGVISPGGVGYDINCGV
                     RLVKTGLVFEQIKDKLTRIVETLFSLIPSGVGSEGVIAKLSKQDQKRLLRDGARWAVA
                     EGYGNHEDLSHMEEGGRLASADAELISDRALERGLKQVGTLGSGNHFLEIGRVEEIYL
                     PQAADTFGLFPDQVVILIHTGSRGLGYQTCEDSLKVMNKAMSAYGISLPDRQLACTPI
                     QSPEGQDYLAAMAAAANYAWANRQVIMSQAEKAFMKALNISPKDLGFALLYDVCHNIA
                     KIEEHTVDGKKRRLCVHRKGATRAFAPGHPQIPADYQNVGQPVLIPGDMGRYSYLLVG
                     TESAMEKTFGSTCHGAGRVMSRAQSKKQSQAINIDQEMARRGVVARYQGRGTMAEEMP
                     HAYKDVADVVETMDDAGISKRVARFRPVGVIKG"
     gene            3143..3535
                     /locus_tag="VFN58_04915"
     CDS             3143..3535
                     /locus_tag="VFN58_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917365.1"
                     /translation="MPKIKHLAIRTEDTGKLAEFYMNVFEMQMLHKGKDEGGAIFLTD
                     GYFNLAILPNHEQNAPNGLYHFGFEVENGEKIVERMKKVNPNKLPKARPNGRPYAETR
                     GSDPDGNYFDISEHGFSVVVPLGEKPSS"
     gene            3642..>4053
                     /locus_tag="VFN58_04920"
     CDS             3642..>4053
                     /locus_tag="VFN58_04920"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917366.1"
                     /translation="MSTVIIRSAFSCLLLASLVLASVCAPPFLNAQIKSNLSSSVTSE
                     SMTAVWVAREHGLFKKHGLDMQFILMPRNPLAVAALIAGEIDAAIIGPGHLVNAGLGG
                     ADVIGSANFHQKLDFGSTRGPTSRKQRNFGASGSP"
BASE COUNT          924 a         1105 c         1161 g          863 t
ORIGIN      
        1 cagttatcgg acattttcga agagttttgt gccacccggc tgctcgatac attgaacagc
       61 aaaaaagtcg aaaggatccg ttcgcttttt gacgagaaga aggttcacat cttactccag
      121 cacgaccccg atccggacgc cctcggcagc gcgctcgcct tgcgcgagct tttgggacgc
      181 aatcgtatta ccacgccgat tgtgactttt ggcgaagtga ctcggccgga aaatcttgcg
      241 atgatccact tcctggatat ccagatcgat cggatcacct atgaagatct acacaaagac
      301 ggcgcgcagc tggctctggt cgacgtccaa ccgccctatt ttgaccagcc cctcggaaga
      361 gtcgatctgg tggtagatca tcacccgaag cggaccccct tcaaagcccg ttttaccgat
      421 cttcgaaccg gctatggtgc gacgtccacg atcttcacgg agtatttacg ggccatcggg
      481 atggagccgt cgcaaagact ggcgaccgcg ctgctctacg gaatcaagac ggatacactt
      541 tttctcgaac ggggcagcat tctcgctgat ctcagcgcat tcacgtttct ctatcccttt
      601 gccaacaagc cgatgatcac acggatcgag cggcccgcct tgccgcgtga agatctggaa
      661 gctctcgggc gggcgctcag ccgcctgcaa ctggatgacg gggtggccgt gatccacatg
      721 ggcgagatca accgagagga tgtcatcccg cagatggcgg agttttgttt acacatcgag
      781 ggcgtcgagt ggggagtggt atccggattg gtcaaggatc gcgtggtcat ctccgtgcgc
      841 aatgtcggct acgtcaggag cgccggtgat atcatgaaga agctctacga cgacatcggc
      901 agcgccggcg gccatcgcgc gatggccaaa gctgtgatcc ctctggatcg tttcaaggaa
      961 cgttatggcg aggtgagcga gaaagtcatt cggcaagcca tcgtgccaat gatcgtcgaa
     1021 cgcgacgaac aagcagcgca tggctaatga ccgggggact taccgcgaaa tcgaacatac
     1081 cgctgatctg ggggttgaaa tcacggccgc cgacttgccg gcgcttttcg cttcagcggg
     1141 ggaagcgctt tacgccctga tcgccgatcc cgcaacgatt gaaaaccgcg aagctatcaa
     1201 ggtctccgcg accggggcca atcccgagga tttactccat acatggctgt gcgagctgct
     1261 ggcgcttttc aatgtggaag gtttcgtcgg taagcgttgt gagattacgc acatcagcga
     1321 cggacaagtt caagggcgag tcagcggcga aaagttggac ctcaaacgcc atgcctttca
     1381 caccgaaatc aagggtgtta cctatcacga tttcaaagtg tggcaagatg ccggcacttg
     1441 gcatgcccgg gtcatcttcg atgtctagag cacttcatcc tatcgctaaa aggattctgt
     1501 tcgagggatt gaatggcaac tgaggacatc aagctcaatc gtctggaaga ctacctctgg
     1561 gagattcctc gccagggcgg catgagagtt cccggccgta tctacgccag tgaaaaactc
     1621 atggcgggcc ttaaggtaga caacagcctg cgtcaggtca tgaacgtcgc gcatctcccc
     1681 ggcatcgtcg gctattctct cgccatgccg gatatccact ggggctacgg ctttcccatc
     1741 ggcggcgtgg cagcgatcag cgtcgatgac ggcgtcatct ctccgggggg tgtcggctac
     1801 gatattaatt gtggcgttcg tttggtgaaa accggtttgg tcttcgaaca aatcaaagac
     1861 aaactaaccc gcatcgtcga gactctgttt tccttgatcc cttccggtgt tggttcggaa
     1921 ggcgtcattg ccaaactttc caagcaggat cagaaacggc tattgcggga cggcgcgcgg
     1981 tgggctgtag cagaaggcta cggaaaccac gaggatctct cgcacatgga agagggtgga
     2041 agacttgcgt cggccgatgc ggaactcatc agcgatcggg ctttggagcg aggtctcaaa
     2101 caggtgggta ccttgggctc gggaaatcac tttcttgaga tcgggagggt ggaagaaatc
     2161 tatttgccac aggccgcgga tacgttcggg ctatttcctg atcaagtggt cattttgatt
     2221 cacacgggct cgcgcggcct gggttatcag acctgcgaag actcactcaa agtaatgaac
     2281 aaggcgatgt ccgcttatgg gatctcattg ccggatcgcc agctggcgtg cacgccgatt
     2341 caatcgcccg aaggccagga ctacctcgca gccatggctg ccgcggcgaa ctacgcttgg
     2401 gccaatcgac aagtgatcat gagtcaggca gaaaaggctt tcatgaaagc gctcaatatc
     2461 agccccaagg acctcggctt cgcgttgctt tacgatgtct gccacaacat tgccaagatc
     2521 gaagagcata cagtcgatgg gaaaaagcgg cgtctgtgtg tgcatcgcaa aggagcgacg
     2581 cgcgcttttg ccccgggtca tccgcagatt ccggccgact atcagaatgt cggccaaccc
     2641 gttctcatcc ccggagacat gggacgctat tcatatctgc tcgtcggaac tgagagcgcg
     2701 atggaaaaaa ccttcggcag cacctgccac ggcgccggca gagtgatgag ccgcgcccag
     2761 tcaaagaagc aaagccaagc tatcaacatc gaccaagaga tggcccgtcg cggtgtcgtg
     2821 gcccgctatc agggacgcgg taccatggcc gaagagatgc cgcacgccta caaggacgtg
     2881 gcggatgtcg tggagaccat ggatgacgcc ggcatttcaa aacgcgtcgc gcgcttccgg
     2941 ccggtgggcg tgatcaaagg ataaacttag aagatatcct tgcagctctc tcgtggcggc
     3001 catgaacggt gcgctccttc agactaggat ggttcaaggt ccgagacgtt gaaccttgag
     3061 ctttgaacct tgaaccgccg tattacttga caactagcat cagcggggaa taagggttga
     3121 ttaataacag aaggaggttt ctatgcctaa gataaaacat ttggccattc gcacggagga
     3181 cacgggaaag ttagcggagt tctatatgaa tgtctttgag atgcaaatgc tgcacaaagg
     3241 caaggacgaa ggcggcgcca tctttctcac cgacggttac ttcaacctcg ccattcttcc
     3301 caaccatgag cagaatgccc ccaatggtct ctatcatttc ggctttgagg tagagaacgg
     3361 cgagaagatc gtcgagcgga tgaaaaaggt caatcccaac aaattgccga aagcgcgtcc
     3421 caacggacgg ccctatgccg agacccgcgg atctgatccc gatggcaact acttcgatat
     3481 ttcggaacat ggcttttcgg tggtcgtacc cttgggcgaa aaaccgagca gttaattttc
     3541 tcgatcgcta agcacttttc gttctgttcg catggcggcg tgggggaccg gttaaacggc
     3601 tcccccacag ttaagcgagc ggttgcgtga aggctcggcc gatgagtacc gtgataattc
     3661 gtagcgcttt ctcctgcctg ctcttggcga gcctggtgct ggcttccgtc tgcgcacccc
     3721 cattcctgaa cgcgcaaatc aagtcaaact tgtcgtccag cgtcaccagc gaaagcatga
     3781 ccgccgtgtg ggtggcgcgc gagcatggcc tgttcaaaaa gcatggactc gacatgcagt
     3841 ttattttgat gccgcgcaat cctctggccg tcgctgcgct gatcgccggg gagattgacg
     3901 cggcgattat tggcccggga cacctcgtca acgctggtct cggcggcgcg gacgtcatcg
     3961 gcagcgccaa ttttcatcag aagctcgact ttggctcaac acgaggcccg acatcaagaa
     4021 agcagaggaa cttcggggca agcggatcgc cat
//
LOCUS       DASSWZ010000201        10962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_445490, whole genome shotgun sequence.
ACCESSION   DASSWZ010000201 DASSWZ010000000
VERSION     DASSWZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445490"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1758)
                     /locus_tag="VFN58_04925"
     CDS             complement(4..1758)
                     /locus_tag="VFN58_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET8917367.1"
                     /translation="MKNYRRLLQYLKPYVWPYFVLAMVCMVGYSATEGAMPFLVQWMM
                     DDVFVKRDVDMLYYLPPTIIGIFLIRGLLNFGQGYLSDYVGLRIVQDVRNLLARHLQF
                     MSLSFFSRHPSGMLISRVASDVTLLRSALTDALASFLKDTTSLIVLVAVAFIKDWFLA
                     SLAFVVFPASVLPVMRLSRKIKRFARRGQISTGKLTVFLQESIQGNRIVKAFGMEDYE
                     VERFAGENFRLFKQSLRASRVRGIVTPMMELLAAFGIGTVVWYGGSSVVAGGRTPGEF
                     MAFMAAMFFMYQPFRNLTRTYTVLHQGLAGAERVFEILDEPPSIADKPDAEMAPRFAK
                     AIEFHDVSFAYGAKPVLSRINLTIRAGEVVALVGMSGAGKSTLADLIPRFYDVNSGQI
                     TIDGVDIRDVGLASLRSQIGIVTQHTFLFDDTVRNNIAYGDPERRIDDVIAAAKAAHA
                     DEFIRSMPNGYDSQIGEMGMQLSGGQRQRLAIARALLKDAPILILDEATSALDSDSER
                     MVQEALEHLMTERTTLVIAHRLSTIRNADRIVVLVDGTIAEEGTHEQLLARKSEYSRL
                     YSLQMLEEERSLDGETLH"
     gene            complement(1768..2952)
                     /gene="lpxB"
                     /locus_tag="VFN58_04930"
     CDS             complement(1768..2952)
                     /gene="lpxB"
                     /locus_tag="VFN58_04930"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET8917368.1"
                     /translation="MFFIIRFQMAAQSKQVMLVVGEASGDIHGARLVRALGDKDQTLR
                     FFGVGGEQLERTNFEVLFNVSQLAGMGFVELAGSMRNIWRADRILRQAMRERRPDLLV
                     LIDFPEFNLRLAKLAKKLKIPVLYYISPQIWAWRRGRVRQIARCVDHMAVVFPFEVPF
                     YEKWGVRVSFVGHPLLDIVRSREPREGVLAQLALDAEKPTIAILPGSRRGEILYHLPV
                     LLDAAFRLSQDSAVQFLVIRASTVDRGELESMLKRVSLRIPIVEDQRYDAVNACDLAW
                     TASGTATVETALLLKPMIIVYRLSWLTYALARMLVNVKHVGMVNIMAGKEVVPELIQA
                     DFTAERVVKKTRTLLENRDRRDRIVGKLVSLREKLGTPGAADRVAHIAMSMMGKGSSV
                     TL"
     gene            complement(2954..3886)
                     /locus_tag="VFN58_04935"
     CDS             complement(2954..3886)
                     /locus_tag="VFN58_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET8917369.1"
                     /translation="MPDQSKIAAAVVGVGYLGKFHAEKYADSPKATLVAVVDIDKGRA
                     ESVADQLGVAAFTDYRELLGRVECISVAVPTRLHYEVAGYFLKRAVDVLVEKPIAAAL
                     QQGRELVELASAQGLILQVGHLERFNPAIRRLENIIQEPRFVECHRLAPFVERGTDVD
                     VVLDLMIHDIDVIASLVRSTVDRVEAVGVAVLTDKPDIANARITFSNGCIANVTASRV
                     SIKRERKIRFFQPDAYLSIDYDQRRAQIFHKPPPGGGWMDIRAETIEIKEADALADEI
                     DSFLDCVRTRSLPLVSGAEGLRALEIASMISGQL"
     gene            complement(3898..4707)
                     /gene="lpxI"
                     /locus_tag="VFN58_04940"
     CDS             complement(3898..4707)
                     /gene="lpxI"
                     /locus_tag="VFN58_04940"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707637.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET8917370.1"
                     /translation="MKKLGLIAGNGKFPLIFAEQARREGFSLVAVAHRGETLGAIEEI
                     IEDVTWIYVGQLGKMIRTFHRAGITEAAMAGGIQKVKFLENFRPDLRGARFLARMKSK
                     DDDALLRGVAEELESEGIKILDSTLCLSQIVASEGVLTKRSPRATEWEDVRFGFGVAK
                     EVGRLGIGQTIVVKKQMIVAVEALEGTDAAIERGGKLARPGFVVVKVSKPQQDLRFDV
                     PAVGVDTVRQLHEMGGTVLAVEAGKTILLEKEALLKEAEDLGISVVALRDG"
     gene            complement(4760..5536)
                     /gene="lpxA"
                     /locus_tag="VFN58_04945"
     CDS             complement(4760..5536)
                     /gene="lpxA"
                     /locus_tag="VFN58_04945"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET8917371.1"
                     /translation="MTRIHETALVDRRAEIDSDVEVGPYSVVGACVKIGKGSRIKSHV
                     VIEGRTTLGEGNVIFQFATVGSVPQDLKYKGEPSELIIGNHNTIREFVSVNPGTTGGG
                     MVTRVGDRNLLMMYCHIAHDCVLGNHNIIANGATLGGHVVIEDYVIVGGLVGIHQFTR
                     VGSGAILGAGSMVSKDVPPYCNATGDRARLHGLNLEGLKRRRVGKEKIEALKKAYRIL
                     FRSRLKTSDAIVKVRQELTGCAEAEQLVTFVQNSQRGICR"
     gene            complement(5565..6002)
                     /gene="fabZ"
                     /locus_tag="VFN58_04950"
     CDS             complement(5565..6002)
                     /gene="fabZ"
                     /locus_tag="VFN58_04950"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET8917372.1"
                     /translation="MLDVKEIFKYLPHRYPFLLVDRIVEIHGEDKIVGIKNVSFNEPF
                     FQGHFPAHPVMPGVLICEAMAQVGAIFAHHARGGLDSGKVFVLTGLERVRFKRPVEPG
                     DQLRLELSCLKRRGSFWKMQGVASVDGKVVAQAEISAMEIEML"
     gene            complement(5989..7065)
                     /gene="lpxD"
                     /locus_tag="VFN58_04955"
     CDS             complement(5989..7065)
                     /gene="lpxD"
                     /locus_tag="VFN58_04955"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET8917373.1"
                     /translation="MTAPGKTLAELAQQVGGRVIGDGRIVIRKVASIEEAGPGEITFL
                     ANPRYQPLLASCRASAVIIGPGIVTESPTGHPRVYLEAADPYVAFAKILQLFNPSASY
                     GEQISPKATIDPAANLAVGVTVFPHVFVGRGTWVGARTVLYPGVFLGEQVQVGQDCVL
                     HPNVVVRDGCRVGNRVVLHAGVVIGSDGFGYAGEGNQRVKIPQVGIVEVEDDVEIGAN
                     TTVDRATLGRTTIGRGVKIDNLVQIAHNVVVGENSLIAAQAGIAGSTRIGRDVMLGGS
                     VGIINHLTIGDGARIGPGSGILRSVPPGVMRSSGLDAAPHREWLKVITLLPQLPRLWS
                     AVRHLEKKVSELVKSCNKETHARR"
     gene            complement(7062..7574)
                     /locus_tag="VFN58_04960"
     CDS             complement(7062..7574)
                     /locus_tag="VFN58_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET8917374.1"
                     /translation="MRYFLAAIWFFLYTGVGWSQDKVKIGFIDVQRAISESQAGKRAK
                     ERFQAQVKKAEADLLREKTEIERLKSDLDKKGALLKEEEKRNLEADLQRRYVNYQRGM
                     QDQQQELRQKEGEMTADILKELEKIVNEVGKAEKFTLILERSQILYSDQGIDITTRVI
                     EAFNARSKSK"
     gene            complement(7633..9912)
                     /gene="bamA"
                     /locus_tag="VFN58_04965"
     CDS             complement(7633..9912)
                     /gene="bamA"
                     /locus_tag="VFN58_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET8917375.1"
                     /translation="MVLKEVKIAGNVRVEEDGIRLHIKNRPGELFDRAVVEQDVKAIY
                     RMGFFEDVQAEISPDGVLTYTVKEKPYVREVKIQGNSQIGRDKIETALGITPRTILDH
                     GKVTEGVDKVRKLYNEQGYVSAKVDYALSIESSNQAVLVLDIVEGNRLLIKKVSFEGN
                     RTFSEGELRGLMATKEEWIFSFITNRGVLDRDILTNDIAILSNHYYDHGYIDHKIDEP
                     VILRDRDGLEVVIRVDEGQQYRVGKVEIGGDLLQDGKQMLKRVKLTTGQIFRGSRLRE
                     DITTITDMYSDKGFAFVQVDPVTKISAPEKTVDVALVITKGPPVYFNRVLIAGNTKTR
                     DKVVRRELETTEQELYSGSKITQSRNALQRTGYFEDVQLTTKKTAQPDTVDLLVDVKE
                     GPTGTFSIGAGYSGGSGFLFNAGVSEKNLFGRGQSLNGTFAIGSERQDFIVSYNEPYF
                     NDTKVALGITGFNTETDYPDFDERKLGMAVTTSYPLRGFTLPFWGDRKSEASIGSDEL
                     NRDAPLTLWDYMRGTMAYEFTKDTISHIESGAPASITDAKGTSLTSAVIPGFTYDSRD
                     HFFNPTEGTKSLFAVKIAGLGGDSRFVKADIGGRWYYPLLKDPNWGGSYVFALGGALG
                     YGVGYAKSSSSGHDLPLFERYFPGGINSVRGYADRSLGPKEDGDVVGGDKQAIMNVEL
                     LFPIAEQYGLRGVAFFDVGQAFSSSQNISWGEFRRSVGMGARWMSPFGPLRVELGFPL
                     NKKPGDDTSVLGFGAGNLP"
     gene            complement(10103..10780)
                     /locus_tag="VFN58_04970"
     CDS             complement(10103..10780)
                     /locus_tag="VFN58_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8917376.1"
                     /translation="MSNLLSVKNLHKSFVEGGEEIQVLRGLDLELKTGERLAVIGESG
                     VGKSTLLHVLGTLDRPTAGQILYQGKEIPMDDGAALSHFRNREIGFVFQFHYLLPDFT
                     ALENVMFPALIQGIEAQRAKREAEQLLEKVGLKDRVHHRPGKLSGGEQQRVAVARSVI
                     LQPKLILADEPTGSLDLRIGAEVQDLLFRLNEEKGIALIVATHNREFAEKIGRRVELQ
                     TGRLVPA"
     gene            complement(10773..>10962)
                     /locus_tag="VFN58_04975"
     CDS             complement(10773..>10962)
                     /locus_tag="VFN58_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein-releasing system transmembrane
                     subunit LolC"
                     /protein_id="HET8917377.1"
                     /translation="IQKYRFIELPKDVFLISTVPVRIYLSNFLLVGFASLFVCLLASI
                     YPARQAAKLDPVEIIRYE"
BASE COUNT         2620 a         3238 c         2527 g         2577 t
ORIGIN      
        1 aaatcaatga agggtctctc cgtcgagtga cctttcttct tccaacatct gcaaagaata
       61 gagtcggctg tattcgctct tccgcgctaa cagctgttcg tgggttcctt cttcggcgat
      121 ggttccgtca accaagacca cgatacgatc cgcgttccgt atcgtcgata aacgatgggc
      181 gataaccagg gttgtccgtt cagtcatcaa atgctctaga gcttcctgaa ccattcgttc
      241 tgagtccgaa tcgagcgccg aagtggcttc gtcaagaatt agaatcggcg catctttaag
      301 aagtgcccgc gcaatcgcca gcctctgccg ctgcccgccc gacagctgca ttcccatctc
      361 gccgatttga gaatcgtatc catttggcat ggaccttata aactcgtctg catgagctgc
      421 cttggctgcg gcaatgacat cgtctatccg tctttccgga tcgccatagg cgatattgtt
      481 tcgcaccgta tcgtcgaaga gaaacgtgtg ttgcgttacg atgccgattt gagagcgcag
      541 cgatgccaaa cccacgtccc gaatatcgac tccatcaatg gtaatttggc ccgagttcac
      601 atcatagaaa cgaggaatca aatcggctag ggtgctcttc ccggcaccgc tcattccgac
      661 caatgcaacc acttcgccgg ctcggatggt tagattaata cgactcagca ccggcttggc
      721 cccgtaggca aagctcacat catgaaactc aatcgccttc gcaaagcgcg gggccatttc
      781 agcgtctggc ttgtctgcaa tgctcggagg ttcgtctagt atctcaaata cacgctctgc
      841 ccccgcaagt ccctgatgaa gaacagtata ggtccgggtc aagtttctaa acggctggta
      901 catgaagaac atcgctgcca taaaggccat gaattcaccg ggcgtgcggc cacccgcgac
      961 gacgctcgac ccgccgtacc ataccaccgt gccaatccca aaggctgcga gcagctccat
     1021 catcggcgtc acgatacctc tgacgcggct tgctctcagg gattgtttga atagccggaa
     1081 attctccccg gcgaatcttt cgacttcata gtcttccatc ccgaaagctt ttacgattcg
     1141 gttgccttga atgctctcct gcaaaaagac cgtcaacttc ccggtgctaa tctgcccgcg
     1201 cctggcaaat cttttgattt tcctacttag ccgcatgacc ggcaacacag aagccggaaa
     1261 aaccacaaac gccagcgacg ctaagaacca atctttaatg aaggcaaccg cgaccaggac
     1321 gataagcgag gtcgtgtcct tcaaaaacga cgccaaggcg tcggtcaaag ccgaacgcag
     1381 tagggtgaca tcgctggcga ccctggaaat cagcatgccg ctgggatgac gagagaaaaa
     1441 agacagggac atgaattgca gatgtcgggc cagcagattc cggacgtcct ggacgatgcg
     1501 cagtccgaca taatcgctca aataaccttg gccaaaattc agcagtcctc ggatcaaaaa
     1561 gattccgata atcgtcggcg gaaggtagta gagcatgtcc acgtcccttt tgacgaaaac
     1621 gtcatccatc atccactgga ccaaaaaggg catcgcccct tctgtcgcgc tatagcccac
     1681 catacacacc atcgccaaca cgaagtaggg ccagacatag ggcttcaaat attgcagcaa
     1741 tcggcggtaa ttcttcaaag aaatctccta gagcgtgacg ctggacccct tacccatcat
     1801 ggacatggcg atatgggcga cacggtcggc cgccccaggc gtgcctagtt tttccctcaa
     1861 cgataccagt tttccaacga ttctgtcccg ccgatcccgg ttctccaaca aggttcgcgt
     1921 tttttttaca acccgctccg ccgtaaaatc tgcctggatc agttcaggca cgacttcctt
     1981 gcccgccata atgttcacca tcccaacatg cttgacgttg accagcattc ttgcaagcgc
     2041 ataggtgagc catgacaacc gatagacaat gatcatcggc tttagcagca acgcggtttc
     2101 aacggtggcg gtgcctgatg ccgtccatgc caaatcgcag gcattcacgg cgtcgtaacg
     2161 ttggtcttca acgatcggaa tcctcaacga aacccgtttc aacatcgact caagctctcc
     2221 gcgatctacg gtgctggcgc gaatcaccag gaactgaacc gcagagtcct gactgagacg
     2281 gaatgccgcg tccaataaca ccggcaggtg atacaagatt tcgccacgcc gactccccgg
     2341 tagaatggca atcgttggct tctctgcatc caaggcgagt tgtgcgagca caccttcacg
     2401 aggctctcgg gagcgaacga tatcgaggag ggggtggccg acaaagctaa ctctgactcc
     2461 ccatttttca taaaagggca cttcaaaggg aaacacaacg gccatatgat cgacgcatct
     2521 ggcgatctga cgaacccggc cgcgtcgcca tgcccaaatc tgcggactga tatagtaaag
     2581 aacgggtatc ttcagtttct ttgcgagctt tgccaagcgc aaattgaatt ccggaaaatc
     2641 aatcaatacc aataggtcgg gtcttcgctc gcgcatggct tggcgtaaga tacgatcggc
     2701 ccgccaaatg ttgcgcatgc taccggcaag ttccacaaat cccatgccgg cgagttgtga
     2761 gacgttaaaa agcacttcaa agttagtccg ctctaattgc tcgcccccaa cgccaaaaaa
     2821 tcgcagcgtt tggtccttat cgccgagagc tctgaccaaa cgggctccat gaatatctcc
     2881 ggaggcctcc cccacgacca gcatcacttg ttttgattgt gcagccattt ggaaccggat
     2941 gatgaagaac attttacagt tgtccactaa tcatagaagc aatttctagg gcgcgtaatc
     3001 cctcggcacc actcaccaat gggagcgatc gcgttcgtac acaatcgaga aacgaatcga
     3061 tctcgtcggc taaggcatcg gcctctttga tctcgatggt ttcggctcga atgtccatcc
     3121 aacccccgcc tgggggcggc ttatgaaaaa tttgcgcccg ccgctgatca tagtcgatgg
     3181 aaagataggc gtcgggttga aagaacctga tcttccgttc acgctttatg gagacccgac
     3241 tcgcagtgac attagcgata caaccgtttg agaatgtgat tcgtgcgttc gctatgtcgg
     3301 gcttgtccgt cagcaccgcc acccctacgg cttccacccg atcaacggtc gaacgcacca
     3361 ggctggcgat aacatcgatg tcgtggatca tgagatccaa gaccacatcg acatcggtgc
     3421 cgcgctcgac aaacggcgcc agtcgatgac actcgacgaa tctgggttct tgaatgatat
     3481 tttcaaggcg tcggatcgct ggattaaatc gttccagatg tcctacttgc agaatgagtc
     3541 cttgggcgct tgccaactcg accagctctc gaccttgctg aagagcggct gcaatgggct
     3601 tctcgactaa aacatccacc gcgcgtttca ggaaataacc cgcgacctca tagtgcaggc
     3661 gcgtcggaac ggcgacactg atgcactcca ctcgcccgag cagctcccga taatccgtaa
     3721 acgctgctac gcccagttga tccgcgaccg actccgcccg ccctttgtcg atatccacca
     3781 ccgctaccag agttgctttt ggcgaatctg catacttttc cgcatggaat tttcccaagt
     3841 agcctacacc cacaacagct gcggcgattt ttgactgatc aggcactagc cgtacctcta
     3901 tccatcgcgt agcgcgacca ccgaaatgcc caaatcctcg gcctccttaa gtagcgcctc
     3961 cttctccaga agaatcgtct tacccgcctc caccgcgagt accgtcccgc ccatttcatg
     4021 gagctgcctg acggtatcaa caccgacggc cggcacatcg aatcggagat cctgctgtgg
     4081 tttgctgact ttcacgacaa caaaccccgg ccgagccagt ttgccgcctc tctcaatggc
     4141 ggcgtcggtt ccttccaagg cctctacggc aacgatcatt tgcttcttga ccacgatcgt
     4201 ttggccgata cccagacgac cgacttcctt ggccactcca aaaccaaatc ggacatcctc
     4261 ccactccgtc gctctcggcg atcgttttgt caaaactcct tcagaagcaa cgatctgaga
     4321 caaacagagg gttgaatcca aaatcttgat cccttcgctt tccaactctt cggctacacc
     4381 ccgaagtaac gcgtcgtcgt ccttgctctt catccgggcc aaaaacctcg ctcccctcag
     4441 gtccggccga aaattctcca aaaatttgac cttttgaatc ccgcccgcca tggccgcttc
     4501 tgtaatgccg gcgcgatgaa acgtccggat catttttcct aactggccga catagatcca
     4561 agtaacgtct tcgataattt cctcgatggc cccgagcgtt tcaccccgat gcgcaaccgc
     4621 caccagggaa aacccttccc ttcttgcctg ttccgcaaaa atgaggggaa attttccgtt
     4681 gcccgcaatc aaacctaatt ttttcacgga taatcggagc gctcagagta aggagacggg
     4741 atagagacca agattcgtgt taacggcaga tgcctcgctg tgaattttgg acaaaagtta
     4801 ccaattgctc cgcctcggca cagccggtta actcttgccg gactttcacg atcgcatcgc
     4861 tagtcttcaa ccttgatctg aacaaaattc gataagcctt cttcagcgcc tcaatttttt
     4921 cttttccaac tcttcgccgc ttgagacctt ccagatttag cccatgcagc ctggcgcgat
     4981 cacccgttgc attacaatag ggtggaacgt ccttggaaac catcgaaccc gcacccagga
     5041 tagccccgct tccgactctc gtaaattgat gaattccgac gagcccgccg acgatcacat
     5101 agtcttcaat caccacatga cctcccaagg tggcgccgtt agcaatgata ttgtgattgc
     5161 ccaaaacaca gtcatgagcg atatggcaat acatcatcaa aaggttgcga tcgccgaccc
     5221 gggttaccat tccaccgccg gtcgtcccag gattgacgga aacgaactct cgaatggtat
     5281 tgtggttgcc gatgataagc tcgctgggct cccctttgta tttcaaatcc tgcggtacgg
     5341 aacctactgt cgcaaactga aatataacgt ttccctcgcc gagagtagtc cgcccttcga
     5401 tcacgacgtg agatttgata cgagaaccct tgccgatctt tacgcacgca ccgacgactg
     5461 aataaggccc aacctcgaca tcagaatcga tttccgcacg ccggtcaacc agggctgttt
     5521 cgtgaatcct cgtcatcgtc cgaacctacc cttaaccggc gcgcctaaag catctctatt
     5581 tccatggcgg aaatttcagc ttgcgcaaca acttttccat cgacgctagc gacgccttgc
     5641 atcttccaga atgagccgcg acgtttcaaa caggacaact ccaatcgaag ttgatcgccg
     5701 ggctccaccg gtcgcttaaa cctcacacgc tcgagaccgg ttaaaacgaa gactttgccg
     5761 ctgtccaacc cgccgcgcgc gtggtgagca aatatcgcac ctacctgtgc catcgcctcg
     5821 catataagaa ctccaggcat aaccgggtga gcgggaaaat ggccctggaa gaagggttca
     5881 ttgaaggaaa cattcttaat ccctacgatt ttgtcttccc catgtatttc gacgatacga
     5941 tctaccagca aaaacgggta gcggtggggg aggtatttga aaatctcttt aacgtcgagc
     6001 atgggtttcc ttgttacatg acttgactag ctcagacact ttcttttcaa ggtgccgcac
     6061 agcgctccac aatctcggta gttgcggcaa gagtgtgatt accttaagcc attctcgatg
     6121 aggcgcggcg tcgagtccgc tcgagcgcat aactccagga ggaacggacc gaagaatacc
     6181 cgatcccggt ccaatcctgg cgccatcgcc gatagtcaaa tgattgatga ttccgacact
     6241 tccgcccaac atgacgtccc taccgattcg ggtacttccg gctatgcccg cttgcgcggc
     6301 gataagcgaa ttttcgccta caaccacatt gtgtgcgatc tggaccaggt tgtcgatttt
     6361 gacgcctctc ccaatggtcg ttcgtccaag ggtggcgcgg tctacggtgg tgttggcgcc
     6421 gatctccacg tcgtcctcca cttccacaat tcccacttgt gggatcttaa cccgctggtt
     6481 accctccccc gcgtagccaa atccatcact cccgatgacc acgccggcgt gcaacaccac
     6541 tcggtttccg acccgacacc cgtcgcggac aaccacattc ggatggagca cacaatcctg
     6601 tccaacctga acctgttctc ccagaaatac acccggataa agaacggtcc gggctccgac
     6661 ccaagttccc ctacccacaa agacatgagg gaataccgtt acacccactg ccaaattggc
     6721 ggccggatcg atagtcgctt tcggactaat ttgttcaccg tagctcgccg atgggttaaa
     6781 cagctgcaaa attttggcga aagcaacata gggatcggcc gcctctagat acaccctggg
     6841 gtggccggtg ggagactcag taacaatacc ggggcctatg ataaccgcgc tcgccctaca
     6901 ggaagctagc aaaggctgat aacgcgggtt tgcgaggaag gtaatttcac cggggccggc
     6961 ttcttcgatc gacgcaacct tgcggatgac gattcgacca tcgccgataa ctctccctcc
     7021 aacctgttgg gctagctcag ctagagtttt gcctggcgcg gtcacttgga cttacttcgg
     7081 gcattaaaag cttcgatgac acgggttgtg atatcaatgc cttgatcgct gtaaaggatt
     7141 tgcgagcgct ccaagattaa agtaaatttt tccgcctttc ccacttcatt gacgattttc
     7201 tccaattctt tgaggatatc ggcggtcatc tcgccctctt tctggcggag ttcctgctgc
     7261 tgatcctgca tgccccgttg atagttgaca tagcgcctct gaaggtcggc ttcgaggttt
     7321 cgtttctctt cctctttgag aagggcccct ttcttgtcaa gatcgctctt caaccgttct
     7381 atctctgtct tttctctcaa tagatcggcc tcagctttct taacctgcgc ttggaacctt
     7441 tccttagcgc gctttcccgc ttgcgactcg ctgatggcac gttgaacgtc gatgaatccg
     7501 atcttgacct tgtcttgaga ccaaccgact ccagtataaa gaaaaaacca gatagcggct
     7561 aagaaatatc tcacttggat cctccgaatt gctacgattt aggcaggtaa agaaacgaaa
     7621 actcgcgccg cgttacggca gattgccagc cccaaagccg agcaccgaag tgtcatcgcc
     7681 cggttttttg ttaagcggga atccgagttc cactcgcagg gggccaaaag gtgacatcca
     7741 acgcgcgccc attccaacgg acctgcggaa ctcaccccag cttatattct gggacgacga
     7801 aaaggcttga cccacgtcaa aaaaagctac gccccggagc ccgtactgct ccgcgattgg
     7861 aaacagcagc tcaacattca taatggcctg tttgtcacca cccaccacat caccatcttc
     7921 cttgggacct agactcctat cagcataacc gcgcaccgag ttaatccctc cggggaagta
     7981 tcgctcaaag aggggaagat cgtgaccgct gctactcgat tttgcgtatc ccacgccata
     8041 accaagcgcc ccacccaagg caaaaacgta cgatcccccc caattgggat ccttgagcag
     8101 cggataatac cagcggccgc cgatgtcggc ctttacgaag cggctgtcgc cacccaaacc
     8161 agcgatcttg acggcgaaga gcgatttagt tccttccgtg ggattgaaaa aatgatcgcg
     8221 gctgtcatag gtgaaacccg gtatcacggc gctggtcaga gaagttccct ttgcatctgt
     8281 tatggaagcg ggggctcccg attcaatgtg gctgatcgta tctttggtaa attcataggc
     8341 cattgtaccc cgcatgtagt cccatagggt taggggtgca tcgcgattca attcgtcgct
     8401 cccgattgac gcttcgctct ttcggtcccc ccaaaatggc aaggtaaagc cccgtagcgg
     8461 ataactcgta gtcacagcca tgccgagttt tcgctcatca aagtcgggat agtccgtctc
     8521 cgtattaaag ccggtgattc ccaaagcgac cttggtatcg ttgaaatagg gctcgttata
     8581 actgacgata aaatcctgcc gctcggatcc gatggcaaag gttccattga gactttgccc
     8641 gcgcccgaaa agattctttt ccgaaacacc cgcattgaac aagaaaccac taccgccgct
     8701 atatcccgct ccgatgctga aggttccggt gggtccctcc ttcacatcca ccaacaaatc
     8761 aacggtgtcc ggctgagcgg ttttttttgt cgtcaattga acgtcttcaa aatatccggt
     8821 ccgctgaagg gcgttacggc tttgcgtaat cttgctgccg gaatagagtt cttgctccgt
     8881 tgtctccagc tctcgacgca cgaccttgtc cctagtcttg gtattcccgg caatcaagac
     8941 gcggttaaaa taaaccggcg gccctttggt aatcaccaat gctacatcca ccgttttctc
     9001 tggcgcgctg attttagtga ctggatccac ctgcacaaac gcgaagccct tatcggaata
     9061 catatccgtg atcgtggtaa tgtcttcgcg cagacggctt cctcgaaaaa tctgcccggt
     9121 tgtcagcttc acccgcttta acatttgctt gccgtcttgc agaagatccc cgccgatttc
     9181 aaccttgccg acacggtatt gttgtccctc atccacccga atgaccactt caagtccatc
     9241 tcgatcccgg aggataacgg gctcatcgat tttatgatcg atatagccgt ggtcgtaata
     9301 atggttcgat aagatcgcga tatcgttcgt cagaatatca cggtccaaga ctccacggtt
     9361 tgtgatgaaa gagaaaatcc actcctcttt tgtcgccatg agtcccctga gttccccctc
     9421 gctgaaggtt cgattaccct cgaacgaaac ctttttgatc agcagccgat tcccttcaac
     9481 aatatccaat accaaaaccg cttgattgct agactcgatg gaaagcgcat agtccacttt
     9541 agcgctgacg tatccttgct cgttataaag ttttctaacc ttgtcgactc cttcagttac
     9601 tttcccgtgg tcaagaatcg ttctcggagt tatcccaagg gcagtttcga tcttgtcgcg
     9661 gccaatctga gaattgccct gtattttgac ttcccggaca tacggttttt ccttcaccgt
     9721 ataggtcaaa accccatccg gcgaaatttc cgcttgaaca tcttcaaaga aacccattcg
     9781 gtaaatagcc ttgacgtctt gctcaaccac agcccgatcg aatagctctc ctggacggtt
     9841 cttgatgtgt aggcgaatcc catcttcctc caccctcacg ttgccggcaa tcttcacctc
     9901 ttttaagacc actttctcct gtgcaatacc ggtatcaaaa gagccaatac cagcgattac
     9961 ggtaaggaga aaaattacaa aacgaatttg acggaattct gatcgggtgt tcatgacttg
    10021 gagatagcca tggcaactgc cgctaaggcc ttttttgtag caaattcacg tacttttgta
    10081 aagctaattc ttggcttgaa tctcacgcag ggaccaaccg cccggtctgc agctccaccc
    10141 ggcgaccaat tttctcggca aactcccgat tatgagtagc gacaatcaaa gctataccct
    10201 tttcttcatt caagcgaaac aggagatcct ggacttccgc gccgatcctt aaatccaacg
    10261 agcccgtggg ttcgtccgcc aaaatgagct tgggttgcaa aatcaccgag cgcgctacgg
    10321 cgacgcgctg ctgttccccg cctgacaact ttcccgggcg gtgatgcacg cgatctttca
    10381 ggccaacctt ttccagcaat tgctccgcct ctctcttggc acgttgcgct tcaatgccct
    10441 gtatcagcgc cggaaacata acattttcca acgccgtaaa atccggcagc agataatgga
    10501 actgaaagac aaatccgatt tcccgattcc gaaaatggga taaggcggcc ccgtcatcca
    10561 tcggaatctc tttgccctga tacaaaatct ggcccgccgt cggccggtcg agggtgccaa
    10621 ggacatgcag caacgtgctc tttccaacac cggactcacc aatcacggct agccgctcgc
    10681 cggttttcaa ttcaaggtct aacccgcgca aaacctgtat ctcttcaccc ccctcgacaa
    10741 acgatttgtg aagatttttt actgaaagga ggttactcat accggatgat ttcaaccggg
    10801 tcgagttttg ccgcctgccg cgccggatag atgctggcga gaaggcatac aaaaagcgaa
    10861 gcaaaaccga cgagcaagaa attactcaag tagattctca ccggcacggt agagatgaga
    10921 aagacatctt tgggaagctc aatgaaacga tatttttgaa tc
//
LOCUS       DASSWZ010000202         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1252293, whole genome shotgun sequence.
ACCESSION   DASSWZ010000202 DASSWZ010000000
VERSION     DASSWZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252293"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..809
                     /locus_tag="VFN58_04980"
     CDS             345..809
                     /locus_tag="VFN58_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET8917378.1"
                     /translation="MGTQRSSLMNVGRRVDYAVRALTYLAGQPAGKVIRRPEIEKKQD
                     IPSHYLSKIMKDLVAGGLVHSHIGSKGGFSLARPAETINIKEVYETVERPIVLMECLE
                     KGGEYCPYCSVCTQISVWDRAQSLLANYLAGVTISNIADQTGLRERLISLPG"
     gene            864..1079
                     /locus_tag="VFN58_04985"
     CDS             864..1079
                     /locus_tag="VFN58_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917379.1"
                     /translation="MIDLSSEGMKCRAVRDIQSYQGTVSASMEGTIQYEIENLGRHII
                     NVRWDNGLTLNVFPNEIEIIDGDLSWQ"
     gene            1114..1650
                     /gene="nuoB"
                     /locus_tag="VFN58_04990"
     CDS             1114..1650
                     /gene="nuoB"
                     /locus_tag="VFN58_04990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET8917380.1"
                     /translation="MQLINSLPETVFTTKVDDLLNWGRASSQWYMLFGLACCAIELMQ
                     TGGPRADVDRFGAVPRATPRQSDLMIVAGTLTYKMAKRTKLLYDQMPDPKYVISMGSC
                     ANCGGLFQLAYSVCKGVDKIIPVDVYVPGCPPRPEALTEGLLRVQDRIMKEKWLVKMS
                     RDRPEGEIAWPTRSLNHA"
     gene            1620..1889
                     /locus_tag="VFN58_04995"
     CDS             1620..1889
                     /locus_tag="VFN58_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET8917381.1"
                     /translation="MAYTIAEPCIDVKDTACVDVCPVDCIHPTKSEAEAFGQVHQLYI
                     HPEECIDCGACEPVCPVEAIFENDALPEKWKHYAQINADYYKKSE"
     gene            1963..>3232
                     /gene="sufB"
                     /locus_tag="VFN58_05000"
     CDS             1963..>3232
                     /gene="sufB"
                     /locus_tag="VFN58_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET8917382.1"
                     /translation="MTTATNTVEDFIQSEYKYGFVTDIEADTVAPGLNEDVIRLISAK
                     KNEPTFMLEWRLKAYRHWVKLEKSQAEPKWANVHYPAIDYQAISYYSAPKFKADGPKS
                     LEEVDPELLKTYDKLGISLLEQGRLSGVAVDAVFDSVSVATTFKGKLAELGIIFGSFS
                     EAVQEHPELIQKYLGSVVPYSDNFFAALNAAVFTDGSFAYIPKGVRCPMELSTYFRIN
                     AAETGQFERTLIIADEGSYVSYLEGCTAPMRDENQLHAAVVELIALDNAQIKYSTVQN
                     WYPGDKDGKGGIYNFVTKRGKCLGINSKISWTQVETGSAITWKYPSCILQGDSSVGEF
                     YSVALTNNYQQADTGTKMIHIGKNTKSTIISKGISAGHGQNTYRGLVRVMKGATGARN
                     YSQCDSLLLGDKCGAHTFPYLEVLNSSAQVE"
BASE COUNT          817 a          779 c          890 g          746 t
ORIGIN      
        1 agagtatttc taggttattg cggcaaggca caagagacgg ccgagaagct catcggattc
       61 ttacagcagc atgaggtctc ggttcgagaa tacaaaatgg attttgtact tggcagcact
      121 atactcgatc aaatcgatag agccgcgaga ggactccgtc ctgtgttttg aacgtcgagt
      181 atttctcttt gctgcacgca cggtgtcccg acacacgcat ggtagtagcc ttaactctag
      241 ggggatttgc cagatcatgg gtatttgagt cggcgtcttg acaacataga ccttacgggt
      301 ctatctttat tacatcctag cagaagaggc ggagatggtg aaacgtggga acgcaaagaa
      361 gctctctgat gaatgtgggc cgccgggttg actatgcggt ccgtgccctc acttatctgg
      421 ccggtcagcc tgctggcaag gttatccgca ggccggaaat tgaaaagaag caagatatac
      481 cctcgcatta tctctcgaag atcatgaagg atcttgtcgc tggcggactg gttcactctc
      541 acatcggctc caaaggaggg tttagtcttg ctagaccggc agagacgatc aacatcaagg
      601 aggtctatga gaccgtggaa agacccatcg tcctcatgga gtgtcttgag aaaggcggtg
      661 agtattgtcc ttattgctca gtatgcactc agatatcggt ttgggacaga gcgcagagtc
      721 ttttggccaa ttacttggct ggcgtaacta tcagcaatat cgcagaccaa accggtttaa
      781 gagagcggct tattagccta cccggctaaa tttttttcgc cagattgtgg acctagtagg
      841 tcttcttttc tagaggcaca caaatgatcg acttaagctc agagggaatg aaatgccgtg
      901 ccgtccgaga tatccaaagc taccaaggga cggttagcgc ttccatggaa gggaccatcc
      961 agtacgaaat cgaaaacctt ggtcgtcaca tcataaatgt tcgttgggat aacggtctca
     1021 cgctgaatgt atttcccaat gagattgaga tcatcgatgg agatctttcg tggcaatgag
     1081 atcttataaa gcaccttcaa gtaggaggcg tcaatgcaat taataaatag tttgcccgaa
     1141 accgtcttta ccacaaaagt ggacgacctc ctcaactggg ggcgagcatc ttcgcagtgg
     1201 tacatgctat ttggcctggc ctgttgtgcc attgaactca tgcagacggg aggcccacgc
     1261 gccgatgtgg atcgctttgg ggcggttccg cgggctacac ctcggcaatc cgacttgatg
     1321 atcgtagcag gaacactgac ctataagatg gctaagcgta cgaagcttct ctatgaccag
     1381 atgcccgatc ctaaatatgt catctcgatg gggagctgtg ccaactgtgg aggtcttttt
     1441 caacttgcgt attcggtgtg taagggtgtg gacaagatca ttcccgtcga tgtctatgtc
     1501 ccaggatgtc cgccccggcc agaggcgtta actgaggggc tactgagagt tcaggacagg
     1561 attatgaagg aaaagtggtt ggtaaaaatg tcgcgtgatc gaccagaagg agagatagca
     1621 tggcctacac gatcgctgaa ccatgcatag atgtaaaaga taccgcctgt gtggatgttt
     1681 gcccggtcga ttgcatacat ccgacaaaaa gcgaagcgga ggccttcggt caagtacatc
     1741 agctctatat ccaccctgag gagtgcattg attgcggggc ctgcgagcct gtttgtcccg
     1801 ttgaggctat cttcgagaat gacgccctac cggaaaaatg gaagcattac gcccagatta
     1861 acgctgacta ttacaaaaag agcgaataga tctttttcca gttcgctaag gagagcgcat
     1921 ggatcggaag atcgagtctc ataaaaaagg agagttaaaa ccatgaccac tgctaccaac
     1981 accgtcgaag attttatcca gagcgagtac aaatatggct tcgttaccga catcgaagcc
     2041 gacactgtag cgcccgggtt gaacgaagat gtcattcgct tgatttcggc caagaagaac
     2101 gaaccgacct tcatgctgga gtggcgcctc aaggcctacc gccactgggt gaagctggaa
     2161 aagtcgcagg cagagccaaa atgggccaac gtccattatc cggccatcga ctatcaagcg
     2221 atcagctact actcggcacc aaagttcaag gccgatgggc cgaagagctt agaggaagtc
     2281 gatcccgagc tgctgaaaac ctatgacaaa ctcgggattt cattgctaga gcaagggcgg
     2341 ctgtccggcg tcgccgtgga cgcggttttt gacagtgttt cggtggcgac aactttcaaa
     2401 ggaaaattgg ccgagctggg aattattttc ggctctttct cagaggcggt gcaggagcat
     2461 cccgaactga ttcaaaagta tcttggatcg gtggtgccct acagcgacaa cttcttcgcc
     2521 gctttaaacg ccgccgtctt caccgacgga tccttcgctt acatccccaa aggcgtgcgc
     2581 tgtccgatgg agctatcgac ttattttcgc atcaacgcag cggagaccgg ccagtttgag
     2641 cggacgttga tcattgccga tgagggcagc tacgtgagct atctggaggg atgcaccgcg
     2701 ccaatgcggg atgagaatca gttgcatgcc gcggtcgtag agctgatcgc tctcgacaac
     2761 gcgcagatta agtattcgac agtccagaac tggtatcccg gcgataagga cggcaagggt
     2821 gggatctaca attttgtcac caagcgcggc aagtgtctgg ggattaattc caagatctct
     2881 tggacccagg tggagaccgg ctcggcgatc acctggaaat atccgagctg catcctgcag
     2941 ggtgacagct cggttggtga gttttactcg gtggcactca ccaacaatta tcagcaggcg
     3001 gataccggca ccaagatgat ccacatcggg aaaaacacca agagcaccat catctcgaag
     3061 gggatctcgg cgggccacgg gcagaacacc tatcgggggc tcgtgagagt catgaagggt
     3121 gctaccggtg cgcgcaatta ttctcagtgt gactcgttgc tgcttgggga taaatgcggc
     3181 gctcatacct tcccctacct cgaggtactg aacagctcgg cgcaggtgga ac
//
LOCUS       DASSWZ010000203         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_544807, whole genome shotgun sequence.
ACCESSION   DASSWZ010000203 DASSWZ010000000
VERSION     DASSWZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544807"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFN58_05005"
     CDS             complement(<1..259)
                     /locus_tag="VFN58_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET8917383.1"
                     /translation="MNRNQTYRFWRSAGQCLIGGIAVALVTFVCFRLNVSITTAALLY
                     LIVVVLASLTGSFVLSVVVSIIAILCLDYFFTEPLFHIDATL"
     gene            complement(362..>2017)
                     /locus_tag="VFN58_05010"
     CDS             complement(362..>2017)
                     /locus_tag="VFN58_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HET8917384.1"
                     /translation="GDVRQAMQSEGIDVDRATASKALVLATKEQAYLEHGSFHPDWMF
                     TFWKEATQLAMSEGFSALRATGETEWVLRGGRGLERWMEYESRLTHTLSENNCSALCQ
                     YNRRLFPPELILDVIRTHPMVVYGSTVCRNLYYVPPDEFLGTNQTAREVERLLTNIRE
                     RERVEDALREQLTERRRAEEELRRSEAYLAEGQRISHTGSWGWNVATGELFWSREHFR
                     ICGFDPETAKPSYPMFLERIHPKDRLFVEQTVDRAVRERSDLEMDYRIVLTDGSTKYL
                     QSLGHPVIRESGDLVEFVGTVMDVTERKRAEEALREAQAELAHVTRVTTLGELTSSIA
                     HEVNQPLAAVVNNASACLRWLAGQAPNLEEARQSAALIIADGHRAGEIISRIRALARK
                     APPRRDWVNINETILEVIALARSEVHRNRVSTQTQLSSNMPLILGDRIQLQQVILNLI
                     INAIEAMSEISEGPRELQVVTRKDELQGVLVTVRDSGPGLDSDSLDHLFTAFYTTKPQ
                     GMGMGLAISRSIIEAHGGRLWAVPNDGPGATFQFTLPKYQQEA"
BASE COUNT          434 a          576 c          547 g          460 t
ORIGIN      
        1 gcagcgttgc gtctatatgg aaaagcggtt cagtaaagaa gtagtccaaa catagaatgg
       61 cgatgatgga gacaacgacc gatagaacga agctacccgt gagcgatgcc agtacgacga
      121 caatcagata gagaagggcg gccgtagtga tgctgacatt gagtcgaaag cagacaaagg
      181 tcaccaatgc cacagcaatg ccgccaatca gacattgccc ggctgagcgc cagaatcgat
      241 aggtctgatt acggttcatc gagaatgctc attcgatgcg cttgtttcta gcgacatccg
      301 gctccacata agttaattcc ttggagcgag gagattgagc gtaaccaagt atatgagtct
      361 cttaggcctc ctgctggtac ttcggcagag tgaactgaaa cgttgcgccg ggaccatcgt
      421 ttggtactgc ccacagccgc ccgccgtggg cctcgataat cgaacggctg atcgccagtc
      481 ccatccccat gccttgaggc ttggttgtat agaaggcggt gaagagatgg tcgagactgt
      541 ccgaatcgag ccctgggccc gaatctcgca ctgtgaccag cacaccctgc aattcatctt
      601 tcctagtgac gacctgcagc tctcgcggcc cttcgctgat ttcactcatg gcttcgatgg
      661 cgttgatgat caaattgaga atcacttgct gtaattggat ccgatctcct agaatgagcg
      721 gcatgttact cgatagttga gtctgtgttg acacgcgatt cctgtgcact tcgctgcgcg
      781 ccagcgcgat gacttccagg atcgtttcat tgatgtttac ccagtccctg cgtggcggcg
      841 ctttcctggc gagggcgcga atccggctga tgatttcacc ggctcgatgg ccgtctgcga
      901 tgatcagcgc ggcagactgc cgggcctcct ccaggttggg tgcttggccc gccagccaac
      961 gcagacaggc actggcgttg ttgaccaccg cggcaagggg ctgattgact tcatgggcaa
     1021 ttgaggaggt caattctccc aaagtcgtca cccgcgtgac atgcgccagt tccgcctgcg
     1081 cctcgcgcaa ggcctcttca gcccgcttgc gctcggtcac atccatgacc gtaccaacaa
     1141 actcaacgag gtcaccggac tccctgatga cgggatggcc gagactttgg aggtactttg
     1201 tcgatccgtc cgtaagaacg attcgatagt ccatttcaag atcacttctt tcccgaaccg
     1261 ccctatcaac cgtctgctca acgaagagcc gatccttggg gtgaatcctt tctaagaaca
     1321 tgggataaga cggtttggcc gtctcgggat cgaagccaca gatgcgaaaa tgctctcgcg
     1381 accagaagag ctccccggtg gcaacattcc aaccccaact acctgtgtgg cttattctct
     1441 ggccttccgc caggtaggct tcactgcggc gcagctcctc ttccgcgcgc ctgcgctccg
     1501 taagctgttc tcggagagcg tcctctacac gctcgcgttc ccggatattg gtcaacagcc
     1561 tctccacttc acgcgccgtt tggttagttc ccagaaattc gtccggtggc acgtagtata
     1621 gatttcggca gaccgtgctg ccgtaaacga ccatgggatg cgtgcggatg acgtcgagaa
     1681 tgagttccgg tggaaaaaga cgtcgattgt actgacaaag cgcggaacag ttattctccg
     1741 agagggtgtg agtcaatctg ctttcgtatt ccatccagcg ctcaagccca cggccccctc
     1801 tcagaaccca ttctgtttcg ccggtggcgc ggagcgcaga gaacccttcg ctcatcgcca
     1861 actgcgtggc ttccttccag aaggtaaaca tccaatcggg gtgaaaagat ccgtgctcca
     1921 gatatgcctg ctctttggtt gccagaacga gggctttgga tgcggtcgca cgatctacgt
     1981 caattccttc ggactgcatg gcttggcgaa cgtctcc
//
LOCUS       DASSWZ010000204         6163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1054264, whole genome shotgun sequence.
ACCESSION   DASSWZ010000204 DASSWZ010000000
VERSION     DASSWZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054264"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VFN58_05015"
     CDS             <1..1650
                     /locus_tag="VFN58_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HET8917385.1"
                     /translation="VDYMLTITISVASAGDQLFSFLPSAIHGAKLGVEFVLILLLIYL
                     NLRGTKESVLFLLPIFLLFVVAHVFAIGLGVLPKAGEIPAVAATTYQKTIGDIQAMGL
                     WATLFIILHAYSLGGGTYTGIEAVSNGLQALREPRVETGKKTMIYMAASLAFTASGLL
                     LCYLLNQVSPEPGKTLNASLFGKVYGSFFSTDIAYILVVATLVTQAAILLVAAQTGFI
                     DGPRVLSNMAIDSWAPHRFSHLSDNLVTQYGVWFMGLASLGFLAYTGGSVRLLVVMYS
                     INVFLTFTLSQLGMCKHWWEVRQQEELWWRKLLLNGFGLTLTLTILAVTTIVKFAEGG
                     WVTVLVTSGFVFLCYLIRSHYNKVRDALKSLDDALINIPFQPDLKVPVPVRNPGAPTA
                     VVIARDFDGIAIHSLLSIARLFPNHFKNIVFVSVGLIDSGRFKGLSEIENLRLSKEED
                     LKSFVDFGNCLGWYAEYRYSLGTDLIEVLEELCRTVAREFPKSVFFAGKLVFQKENVL
                     ARLLHNHTPFTLQQRLQFEGLDMMILPIRVFAPSGANKTNP"
     gene            1689..3053
                     /locus_tag="VFN58_05020"
     CDS             1689..3053
                     /locus_tag="VFN58_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8917386.1"
                     /translation="MDPVNSRNQLPVLVIDDEKNIRATLSLCLEQMGCQVTAVSSQEG
                     ALTALAQQPYELAFLDLRLGESNGLDLIPKLLAEAPNLAVVVITAYATVDSAVAAIKR
                     GATNYLPKPFTPAQIHHVVEQTVRQRDLLHRIADLEGRLQEAIPDIDLDSSSPKLKYI
                     LDVAAKASASEAAVLLRGESGTGKSVLARLIHSMSRRNRQPFVVVNCPTLSEELLASE
                     MFGHAKGAFTGALRDQAGRVEAAEGGTLFLDEIGEISPSLQAKLLRFLEDKEFERLGE
                     NKTRRANVRTIAATNRDLENHVRNGLFREDLLYRLNVIDLQLPPLRERPEDILRLARR
                     FLAFSAKTARRKVPELSNAAEQALVAYRWPGNIRELRNAIERAVILWPAAIIEPEAFP
                     AHISARARSEAAQLGGNFSLEDIEREHILRVVARTATLEDAATILGIDASTLWRKRKK
                     YEEG"
     gene            complement(3062..3703)
                     /locus_tag="VFN58_05025"
     CDS             complement(3062..3703)
                     /locus_tag="VFN58_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917387.1"
                     /translation="MTETKPRILVADDERNIRKNLAMVLEAGGYQVDEASDGEEALIL
                     CRQTHPDIAFVDLHMPKIEGLDVLAHFRTLSPKTAVVIITAYGSAANAVEAMKLGAVD
                     FLEKPFDPKIIGILAEEILFRQRQGSGGSFDDLMHLADLARERNARLEARAYLKAAMS
                     RAADRPEPYYWLGYLCEVEEDPKRAVQYYYMAVSANHTYEPAVEALKRLGKLH"
     gene            complement(3700..5556)
                     /locus_tag="VFN58_05030"
     CDS             complement(3700..5556)
                     /locus_tag="VFN58_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8917388.1"
                     /translation="MNLYRKLLLAQAPLALALLIVGVVSIFVISFLGSHSQTILKDNY
                     RSVLAAQRMKEAIERMDSAALFIVAGERQKGIEQATKYRPIFEAELKVQEGNITEAGE
                     KEFTEGLRASWTDYLAKFDRLQKTTTPEEARRLYFSELEAAFYKVKVAADEILAINQD
                     AMVRKSDAVRRTAERMNAITLTVALIALMLGLFVSSLLTRRMLRPLSALSEATHKIGE
                     GNFETRAHVRGNDELAHLAHDFNVMAARLGEYRKSSLGDLLQAHLSMQAAIDSLPDPV
                     VIFAVGGDLQNVNQAAQTLLGLISETGVKEPLKAVDASVRSVLERMRSHVLSGKGAYN
                     PRGFEDAVQLPTVLGDRYFLPRAAPVYETRGVVVAATVILQDVTRLRRFEELKNDLVA
                     TVAHEFRTPLTSLRMAVHLVTEQVAGPLTDKQAELLHTAREDCDRLQAMVDDILDLSR
                     IESGKIELYPLPTPVADLIEKAIEENKAEASAKGLHLTVDFPLPEVKVLTDHERIGHV
                     FSNLIGNAVRYTAGEGSVTLGAHVANGTVRFTITDTGKGIAKEYHSRIFEKFFQVPDS
                     GPKGTGLGLYIAREIVWGHGGQIGVESEPGKGSTFWFTLPTAEQPAAKGDRR"
     gene            complement(5553..6068)
                     /locus_tag="VFN58_05035"
     CDS             complement(5553..6068)
                     /locus_tag="VFN58_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET8917389.1"
                     /translation="MLREIALREVAESVDRSVSSNNTTDDAARRAAASERVMVCLPAR
                     PPPHGVLLLRQGSRLAGRLNTDWFVVHVETKQETGHRIDAALQRYLMEDEQRARDLGA
                     EVVRLRSEKPVEALLDFARGHGVRHIVVGRSHKAWWRHLLGQSPVSKLVTQADGFDLH
                     IVSLEDREGKE"
BASE COUNT         1327 a         1803 c         1614 g         1419 t
ORIGIN      
        1 gtcgactaca tgttgacgat tactatctct gtggctagcg cgggggatca gcttttcagc
       61 ttcttgccct cggcgatcca cggcgccaag ctaggcgtcg aatttgttct gattttgctt
      121 ctgatatacc tcaatctacg cggtacaaag gagtcggtcc tctttctgct gccgattttt
      181 ttgctcttcg tggtcgcgca cgtctttgcg attggtctgg gcgtcttgcc caaagcaggg
      241 gaaattcccg cagtcgccgc cacaacttat cagaagacta tcggcgatat ccaagccatg
      301 ggactatggg cgaccctctt tattattttg catgcctaca gccttggagg cggcacgtac
      361 accggtatcg aagcggtcag caacggcctt caggcgctac gcgagcccag agttgagacc
      421 gggaaaaaga ccatgattta catggccgcc tcgcttgcct ttaccgcaag cggtcttctg
      481 ctttgctatt tgctcaacca ggtttcccct gagcccggca agacgctcaa cgccagcctg
      541 tttggaaaag tgtatggtag ctttttctca accgatatcg cctatatttt ggtcgtcgca
      601 accctggtca cccaggcggc cattctgttg gttgcggcac agaccggctt tatcgatggc
      661 ccgcgagttc tctctaacat ggcgatcgat tcctgggcgc cacatcggtt ttctcatctg
      721 agcgacaact tggtaaccca atacggggtg tggtttatgg ggctcgcctc gctgggcttt
      781 ctcgcttata ccggaggcag tgtcaggctc ctggttgtca tgtacagcat caacgtcttt
      841 ctgaccttta cgctctccca attgggcatg tgcaagcatt ggtgggaggt gcggcagcaa
      901 gaagagcttt ggtggcgcaa gcttttgctc aacggtttcg ggcttacctt gacgcttacc
      961 attctggcag tgaccacgat tgtcaagttc gccgagggcg gctgggtcac cgtgctggtg
     1021 acctctggct ttgtctttct gtgttatcta atccggtctc attataataa ggtgcgcgac
     1081 gccttaaaaa gtctggatga cgcgctgata aatatcccat ttcagcccga tctgaaagtt
     1141 ccagtcccgg tcaggaaccc gggagccccc accgcggtcg tgatcgccag ggattttgac
     1201 ggtatcgcaa ttcactccct gctcagcatc gcgcggctgt ttccgaacca cttcaagaac
     1261 atcgtctttg tgtcagtagg cttgatcgat tccggtcgat ttaagggtct gagcgagatc
     1321 gagaacctgc gcctttcgaa ggaagaggac ctcaagagtt tcgtggactt tggcaactgt
     1381 ttgggttggt atgccgagta ccgctattca ctcggaaccg atctcatcga agtattggaa
     1441 gagctgtgca ggaccgtagc ccgtgaattt cccaagtcgg tcttctttgc tggaaaactg
     1501 gttttccaga aggaaaacgt cttggctcgc ctgctccata accacacgcc ctttaccttg
     1561 cagcagcggc tccaattcga aggcctcgac atgatgatct tgcctatccg cgtcttcgct
     1621 ccctcagggg ctaataaaac gaacccttga aactggcgcg ctccagagca tagactgtgc
     1681 agctaagaat ggatcctgtt aattctcgta accagctccc ggtgcttgtc atcgacgatg
     1741 agaagaacat tcgcgccacc ctgtcgctct gtcttgaaca aatgggttgc caagtgactg
     1801 ctgtgtcttc tcaggaaggc gctttgacgg cgctggcgca acaaccctat gaactagcgt
     1861 ttctcgatct gcgtctcggt gaatcgaacg gtctggactt gattcccaag ctgttggctg
     1921 aagccccgaa cctcgccgta gttgtcatca ccgcatatgc aacggtcgat agcgccgtcg
     1981 cggcgatcaa gcgcggcgcg acaaattatt tgcccaagcc cttcacgccc gcgcagattc
     2041 accacgtcgt agaacagacc gtaagacagc gtgacctcct gcaccgaatt gccgatctcg
     2101 aaggacgatt acaggaagcg atcccggata tcgatctcga cagcagctcg ccgaaactca
     2161 agtatattct cgacgtcgcg gccaaagcct cagcctcgga ggctgccgta ctcctccgtg
     2221 gcgaaagcgg cacgggcaag agcgtactgg cgcgcctaat tcattccatg agtcgacgaa
     2281 accgccaacc gtttgtcgtc gttaactgtc caacattatc ggaagaactt ctcgcgagcg
     2341 aaatgttcgg ccatgccaaa ggagcgttca cgggcgccct gcgcgaccag gcgggtcgcg
     2401 tcgaagccgc ggaagggggc acgctatttc tggatgaaat cggcgaaatt tccccgtctt
     2461 tgcaagccaa gttgcttcgc ttccttgaag ataaagaatt cgagcgtcta ggcgaaaata
     2521 aaaccaggcg tgccaacgtg cgaacgattg ccgccaccaa tcgtgacctg gaaaatcatg
     2581 tccgaaacgg tcttttccgg gaggaccttc tgtaccgtct caacgtgatc gacttgcagt
     2641 tacctccgtt acgcgaacgg cctgaagata tcttgcgctt ggcgcgccgt tttctcgcat
     2701 tctccgcgaa aacggcacga cgaaaagtac cggagttgtc aaacgctgcg gagcaggcgc
     2761 ttgtcgctta tcgctggcct ggcaacatcc gggaactcag aaacgcaatc gaaagagcgg
     2821 tgatactttg gcccgcggcg atcattgagc cggaagcgtt tcccgcgcat atctcagcgc
     2881 gtgctcgctc ggaagcggcg caattgggtg gtaacttttc cttggaagat atcgagcggg
     2941 agcatatttt gcgagtagtt gctcgaacag cgacgctcga agacgcagca acgatcctag
     3001 gaatcgatgc ctcaacccta tggaggaaac gaaaaaaata cgaggaagga tgaagcagtt
     3061 cctagtgaag cttaccgagg cgcttcagcg cttcaaccgc gggctcatac gtatgattcg
     3121 cgcttaccgc catataatag tattggaccg ctcgttttgg gtcctcttca acttcgcaga
     3181 gataaccgag ccaatagtaa ggctccgggc ggtcggcggc acgcgacatg gcagccttga
     3241 gataagcgcg cgcctcgaga cgagcatttc gttcccgggc gagatcagcc agatgcatca
     3301 agtcatcgaa tgagcctccg gaaccctgac gttgacgaaa tagaatctcc tccgctagaa
     3361 ttccgataat tttggggtca aacggttttt cgagaaaatc gacggcgccg agcttcatcg
     3421 cttctaccgc attcgccgca gagccatacg ctgtaataat gactacagca gttttggggc
     3481 ttaaggttcg aaagtgagcc agcacatcca atccctctat cttcggcatg tgcagatcca
     3541 caaaggcaat atcgggatgg gtctgccggc acagaatcaa agcctcctcg ccgtcgctgg
     3601 cttcatcaac ttggtaaccc ccggcctcaa ggaccatcgc caggttcttg cgaatattcc
     3661 gctcatcgtc ggctactaga atacgcggct ttgtttccgt catcgcctgt ctcctttggc
     3721 cgcaggctgt tccgctgtgg ggagcgtgaa ccaaaaagtg ctccccttcc ctggttcgct
     3781 ctccactccg atttgccctc cgtgccccca gacaatttct ctggcgatat atagtcctaa
     3841 gcccgtccct ttcggtccgc tgtcggggac ctgaaagaac ttctcaaaaa ttcgcgaatg
     3901 gtattccttg gcaattcctt tgcccgtatc ggtgatcgtg aaccggaccg tcccattggc
     3961 tacgtgggcg cccaatgtga cacttccttc ccccgccgta tagcgcacgg cattaccgat
     4021 aagattggag aatacatgtc cgatccgctc gtgatccgtc aacaccttga cttcgggaag
     4081 cgggaaatcc accgtcaaat gtaagccttt agcactcgcc tccgccttat tctcttcgat
     4141 ggctttctcg atgagatcgg caacgggagt cggcaatggg tatagctcga tcttgccgga
     4201 ctcgatccgc gacaaatcca agatatcatc gaccatcgct tgcaggcggt cgcagtcttc
     4261 tctggcggta tggagcagtt cggcctgctt atccgtcagc ggcccggcca cttgctccgt
     4321 gaccaggtgg accgccatgc gcaacgaagt gagcggcgtc cgaaactcat gggcaacagt
     4381 tgccaccaaa tcattcttca gctcctcgaa acgtcgcagc cgggtcacgt cctgcaaaat
     4441 cacagtggca gcgacgacga cgcctcgcgt ctcataaacc ggggctgctc gcggcaggaa
     4501 atagcggtcg cccaacacgg tgggaagctg caccgcatcc tcgaagcctc ttgggttgta
     4561 agcgccctta ccggagagaa cgtgactacg catgcgctcc aggacgcttc gcactgaagc
     4621 gtccacggct ttaagcggtt ccttgacccc tgtttcggag atgagtccca gaagcgtttg
     4681 tgccgcctga ttgacgttct gcaagtcgcc tccaactgcg aagatcacca ccgggtccgg
     4741 cagactgtca atggccgcct gcatgctgag gtgggcttgc agcagatcac ccagggaact
     4801 cttgcggtat tcacccaagc gtgcggccat aacgttgaag tcatgggcca aatgcgccag
     4861 ctcgtcgttg ccgcgaacgt gggcgcgggt ctcaaaattg ccttccccta tcttgtgagt
     4921 ggcctcgctg agcgcggata acggccgtaa catgcggcgc gtcaataggc ttgaaacgaa
     4981 tagacccaac atcaatgcga tcagagccac ggtaagcgta atggcattca tgcgttccgc
     5041 ggttcgacgc accgcgtcac tcttgcgcac catcgcatcc tggttgattg ccaggatctc
     5101 gtccgccgct actttcacct tgtagaatgc cgcttctagc tcggaaaaat agagccgcct
     5161 tgcctcttcg ggagttgtgg ttttctgcaa acgatcgaac ttggccagat agtccgtcca
     5221 agaggcgcga agtccctcgg tgaattcctt ttctcccgcc tcggtgatat ttccctcctg
     5281 gactttaagc tcggcctcaa aaatgggtct atacttcgtc gcttgctcga ttcccttctg
     5341 gcgctcgcct gcgacgataa acagcgcggc actatccatg cgctcgatgg cttctttcat
     5401 ccgctgggct gcaagaacgc tccgatagtt gtctttcaaa atggtctgag aatgcgaccc
     5461 aagaaacgag atgacaaaaa tagaaaccac acccacaatc aacagcgcga gagcgagggg
     5521 agcttgagcc agcaagagtt ttctgtacag gttcattcct tgccctcccg atcttccaaa
     5581 gagacgatat gaagatcaaa cccatccgcc tgggtaacca gcttggacac cggcgattgg
     5641 cccaagagat gacgccacca agctttgtgc gaacggccca caacaatgtg ccggaccccg
     5701 tgcccgcgag cgaaatcgag cagcgcttcc accggctttt ccgaacggag gcgcaccacc
     5761 tcagcaccta gatcccgtgc tctctgctcg tcctccataa gatagcgctg caaagcggca
     5821 tcgatgcgat gacccgtttc ctgtttggtc tccacatgaa ccacaaacca gtcggtattg
     5881 agacgtcccg ccagccgtga accctgccga agcaacaaaa cgccgtgcgg cggcggccgc
     5941 gccggcagac aaaccattac gcgttcgctg gccgcggcgc ggcgcgcagc atcgtctgtt
     6001 gtattgttgg acgaaaccga ccgatcaaca ctctccgcga cttcccggag cgcgatttcc
     6061 ctcagcaccg caagcttttc cggttggaag aaattttcca gcgcccagga aaccttatcc
     6121 ggcgcctaga tctttcctgc ccgcagccga tcgatcagat ctt
//
LOCUS       DASSWZ010000205         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_191332, whole genome shotgun sequence.
ACCESSION   DASSWZ010000205 DASSWZ010000000
VERSION     DASSWZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191332"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="phnE"
                     /locus_tag="VFN58_05040"
     CDS             complement(<1..930)
                     /gene="phnE"
                     /locus_tag="VFN58_05040"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HET8917390.1"
                     /translation="MAQQTIREGERDIFLEAPPAPRREARFSVFVAVALLVLAWSFQG
                     AKLRPGELIQGIPQIAVTVGRMLPPDFSRVTDAKSYFFPENLSLTELLLPLPLTESQA
                     RSKQRWWDNTFPQTVVGATLETVQMALAGTFLALFVAFPVGFLAARNTTPHPLVYHAV
                     RAALNFLRTIPDLALGLVFVAAVGLGAFAGTLALAIHTATVLGKLLSESVENIDEGII
                     EAIRATGAGYAQILSFAVLPQILPDLISFTLYRLETNIRAASVLGLIGAGGIGYLMST
                     SFRTFQYQEAAAIVVVLIALVMLVDYLSSRLRNL"
     gene            complement(1059..1838)
                     /gene="phnC"
                     /locus_tag="VFN58_05045"
     CDS             complement(1059..1838)
                     /gene="phnC"
                     /locus_tag="VFN58_05045"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HET8917391.1"
                     /translation="MTILEIKDLHKAYGEKTQVLRGINLQIEQGEFVCIIGLSGAGKS
                     TLLRCVNRLIEASSGAILVPRALSGLGVDGAKADVLKLNTEELRLLRRKVGMVFQQFN
                     IVKRLSVIENVLSGGLGYQPALRSVLRIFSREEKRQALTNLKRVGLLDHAYKRADQLS
                     GGEQQRVAIARTLMQQPAIILADEPVASLDPRLSRVVLDILKRVCREDGITALVSLHV
                     LELTREYADRIIGLKQGQIFFDGPARDLTDTIVDSVYHRTD"
     gene            complement(1835..>2651)
                     /locus_tag="VFN58_05050"
     CDS             complement(1835..>2651)
                     /locus_tag="VFN58_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8917392.1"
                     /translation="GFVPAENAQQVIQNAQPIVDILQNRLGMEVQPFVATDYTGVIEA
                     LRGNKLDIAFLTPASYVLAKNEANVKVVLKSERKGSPFYYAAIITRADSGIKRLEDLR
                     GKTFAFGDPLSTSANVFPRKMLHEHGIDPVRDFKQILNSGGHDATVLAVLNGKVDAGA
                     TYANSPDSNDTAWMRYLKNPDDVKKIRAIAFSEPIPADNLVINANLDDRTAKKIEEIF
                     IDLSHDEKGKKMLRDLYQIDGFVAATDKDYDSVRQAFAIAGIPIKEALQKKQP"
BASE COUNT          619 a          743 c          718 g          571 t
ORIGIN      
        1 caagtttcta agacgcgagc tcaggtagtc caccaacatg accagcgcga tcagaacaac
       61 tacgatggcg gcggcttctt gatactgaaa cgtccgaaaa cttgtgctca tcaagtaacc
      121 gatgccgccg gcgccgatca ggcccaagac cgaggcggca cgaatgttgg tctccagccg
      181 atacaaggta aacgagatca gatccggcaa tatttgcggc agtaccgcaa aggaaagaat
      241 ctgggcgtag ccggctccgg tggcgcggat cgcttcgatg atgccttcat cgatattttc
      301 caccgattcc gacagcagct tcccgagcac ggttgcggta tgaatcgcca atgccaaggt
      361 gccggcaaag gcgccgagtc ccaccgccgc cacgaagacc aggcccaacg cgaggtctgg
      421 aatagtgcgg agaaaattca gcgcagcgcg cacggcatgg tagacaagcg gatgcggcgt
      481 ggtgttgcgt gcggcgagaa aaccaacggg aaaggcaaca aacaacgcga ggaacgttcc
      541 ggctaaagcc atctgcacgg tctccaaggt ggcgcccacc acggtttgcg ggaaggtatt
      601 gtcccaccag cgctgtttgc tgcgtgcctg ggattcggta aggggcaacg gtagcaagag
      661 ttccgtcaac gacaaatttt ctggaaaaaa ataactcttg gcgtcagtca cccgagaaaa
      721 atcaggtggc agcatgcggc ctacagtaac ggcgatttgc gggatgcctt gaatcaattc
      781 cccgggcctg agcttcgccc cttgaaagga ccacgccaat actagcaaag cgacggcaac
      841 gaaaacggaa aatcttgctt cccgccttgg cgccggcgga gcctcaagaa agatatccct
      901 ctcgccttct ctgatggttt gctgagccaa aggttgtccc tcctaagctt tgcgatgaat
      961 aaaaacccgc ggatagttgg ccgcgggcga tgggtcaccg cagcaacaaa acccgatcgc
     1021 tggtttccgg cactgagatc gcgacttaaa attcagactt aatccgttcg gtgatagacc
     1081 gaatcgacga tcgtatcggt caagtccctc gccgggccgt caaagaagat ctgtccttgc
     1141 ttcagtccaa taatacgatc cgcgtattcc cgggtcagct ccaagacgtg aagactgacc
     1201 agggcggtaa ttccgtcctc ccggcaaacc cgctttagaa tatctaagac caccctggac
     1261 agtcgaggat cgagactggc gaccggttcg tcagccagga taatggccgg ctgctgcatc
     1321 agggttcgag cgatagccac ccgctgctgc tccccgccac tcaactggtc ggcgcgtttg
     1381 taggcatgat cgagcagacc cacgcgcttg agattagtca atgcctggcg tttttcttcc
     1441 cgcgaaaata tcctcagcac gcttctcagg gccggctggt agcccaaacc acccgacaga
     1501 acattctcga tcaccgagag acgcttgacg atattgaact gctggaacac catgccgact
     1561 ttccggcgca gcaaacgtag ctcctcggtg ttgagcttca aaacatcagc cttagcaccg
     1621 tcgacaccga gtcccgacag cgcgcggggc accagaatcg caccggagct cgcctcgatc
     1681 aggcggttga cgcaacggag caaggtcgat tttcccgcgc cgctcaggcc gatgatgcag
     1741 acaaactcac cttgttcgat ctggagattg ataccccgca gcacctgcgt cttttcaccg
     1801 taggctttat ggagatcttt gatctcaagg atggtcacgg ttgcttcttt tgcagcgctt
     1861 ccttgatcgg gatgcccgcg atcgcaaagg cttgtcgcac cgagtcgtaa tctttatctg
     1921 tagctgcaac gaaaccatcg atttgataga gatcccttaa catcttcttg cctttttcat
     1981 catggctcag gtctataaag atctcttcga ttttcttggc ggttcgatcg tccaggttgg
     2041 cattgataac cagattatcc gccgggatcg gttcggaaaa ggcgatggcg cggatcttct
     2101 tgacgtcgtc agggttcttc aaatagcgca tccaggcagt atcgttgctg tctggggagt
     2161 tcgcatacgt agcgccggcg tcgactttgc cattgagcac cgccagaacc gtggcgtcat
     2221 gaccgccgga attaagaatc tgtttgaaat cccgcaccgg atcgatccca tgttcgtgga
     2281 gcatcttacg gggaaagaca ttggccgaag tcgaaagcgg atcgccgaag gcgaaggttt
     2341 tcccgcgcag atcttccagt cttttgatac cgctgtcggc gcgcgtgata atggcggcat
     2401 aataaaaggg tgaacctttg cgctccgact tgagcacgac ttttacgttg gcctcatttt
     2461 ttgccagcac ataggacgcc ggcgtcaaaa acgcgatatc gagtttatta cctcgcagcg
     2521 cctcaatgac tccggtgtag tctgttgcaa cgaaaggttg cacttccata cccaagcggt
     2581 tttggagaat atcaacgatc ggctgggcat tttgaatgac ttgctgggca ttctccgcgg
     2641 gcacgaaacc g
//
LOCUS       DASSWZ010000206         3827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_191390, whole genome shotgun sequence.
ACCESSION   DASSWZ010000206 DASSWZ010000000
VERSION     DASSWZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191390"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1082..1156)
                     /locus_tag="VFN58_05055"
     tRNA            complement(1082..1156)
                     /locus_tag="VFN58_05055"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1121..1123),aa:Glu,seq:ctc)
     gene            complement(1158..1233)
                     /locus_tag="VFN58_05060"
     tRNA            complement(1158..1233)
                     /locus_tag="VFN58_05060"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1198..1200),aa:Gln,seq:ctg)
     gene            complement(1299..2714)
                     /gene="gltX"
                     /locus_tag="VFN58_05065"
     CDS             complement(1299..2714)
                     /gene="gltX"
                     /locus_tag="VFN58_05065"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET8917393.1"
                     /translation="MAQVRTRFAPSPTGYLHIGGARTALFNYLFARHNGGKFILRIED
                     TDRERSTPEAIKAILEAMNWLGLDWDQGPFYQTERYPLYKEKIQQLLAEGKAYPCICT
                     SLELDAKRQLAQKEKRKPMYDGTCRPPEGVIPPLPKDKPYTVRFRSPRDGLTIVEDTV
                     KGQVIFDNRELDDLIIARSDGTPTYNFCVVVDDIDMNITHIIRGDDHLANTPRQMLLY
                     RALAQIPPQFAHVPLILGTDKARLSKRHGATSVMAYRDMGYFPEAVLNYLVRLGWSHG
                     DQEIFSRDELIEKFSLESVGKSAGVFNPEKFLWVNSHYLKERPLSQLAEDVIPYIAAR
                     GYPVPQDKSWLEKMVKTLRERSKTLVELVDSAGFYLTDDISIDEKAATNFLTLEVRAP
                     LAKLIEKLTGLDDFTEANIEQAFSAVLEELGLSMSKLAQPVRVALTGSTVSPGIHEVI
                     AVLGKERTLQRLRSALQRLQP"
     gene            2883..>3827
                     /gene="glnS"
                     /locus_tag="VFN58_05070"
     CDS             2883..>3827
                     /gene="glnS"
                     /locus_tag="VFN58_05070"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HET8917394.1"
                     /translation="MVPAEGTPSHFIRNIVIEDLQQDKNGGRVHTRFPPEPNGYLHIG
                     HAKSICLNFGLAAEFNGLCNLRFDDTNPSKEDVEYVESIKADVRWLGFDWGDREYFAS
                     DYFPQLYQFAIQLIKAGKAYVCDLNADQIREYRGTLTEPGKNSPYRERSVEENLDLFE
                     RMKAGEFPDGARTLRAKIDMASPNLNMRDPVMYRILHATHHRTGDEWCIYPTYDFAHG
                     QSDSIEGITHSICTLEFEDHRPLYDWFLEQLAIHHPQQIEFARLNLTYTALSKRKLLQ
                     LVQQGHVSGWDDPRMPTLAGFRRRGYTPEAIRIFCERIG"
BASE COUNT          965 a          955 c         1051 g          856 t
ORIGIN      
        1 cctgtacgag ggaaacggaa aaagtcgaga caaagaaccg gagcgctgcg gaaagctctt
       61 tcacgtgggt gtgtggcgac caaatatggt ggccccaacg ggattcgaac ccgtccctgc
      121 aaaaaaggac cgtagcaaga agcaatagaa aatcaaccgt atagagtgaa acgagcgcga
      181 ggggtagcat tggacgaatc cctaaacgcc ctctaaagcg aggaccgaag tagaaactaa
      241 aaacgggcgg gctgtgcgcg aacacctgcg cccccaaagc cgaaatcacc aaatcactca
      301 agtaataaat caccgagtat caacaccctg cccgccgcgc acaaaccaga ctaagaaggg
      361 gatgctctta tcgctgacgc ccacggtaac cagtagtgga ccatgcacgt tggaaaaggg
      421 atggccggag ggaagcgtag gttcaaactt agatgggtct accaggctcg ccaggtgtca
      481 atcgaatggc gccgaatgtc aagacacact ctttttaatg gattattttt agtcctaaga
      541 aggagatttc cagattggat cgggagcgcg agaagatgga catgggggat gattcacatg
      601 ctgattttct actgtaacca ctgtgacgag ttgcttacag gcggcgacta cgaaagtacg
      661 gatcagatcg tagacagact tgaagagcat ataaagaaat gccccccggc agtatttgcc
      721 ttcgagggaa caacgagcgt tgccacgcag agacttgacg ctttacgatc attccatgag
      781 ggtgagcgcc gtgcgggtaa gatacggcta cactgaccat catgcccgct tttcaatcct
      841 ccgacttaaa gtttgcgaca acacgcgcca tacgtacaca gcgttgcctt aacgctaggt
      901 tgtaatccga agtggatagc ggagcaaact gcaacgagtc tcgccatgat tcaacagaac
      961 tgggaagtac atccgtgatg atggcgatgc actcttgcgg gcctacgtta aaaggccaga
     1021 aacgagctaa aaccagagac cggaaccttt ccgggtggga ggactaacta cgcgggatag
     1081 ttggtggtcc caacgggatt cgaacccgtg ttaccgacgt gagaggccgg tgtcctaacc
     1141 actagacgat gggaccttgg ctgaggaggt aggactcgaa cctacaatcg cctgatccag
     1201 agtcaggtgg cttaccagtt agccgactcc tcaaatttga cggcgaagtt aaactagaag
     1261 aattacccag gagagtcaag ttggaaccgc ggggcaagct agggttgaag ccgttgtagg
     1321 gctgatcgta gacgttgaag cgttcgttcc ttgcccagca cggcaattac ctcatgaatg
     1381 ccgggactga cggtcgagcc ggttagtgct acgcggaccg gttgagcgag cttgctcatg
     1441 gataaaccca gctcttctag taccgcggaa aatgcctgtt caatattggc ctcggtgaag
     1501 tcatccagcc ctgttagctt ttcgattagt ttggcgagcg gcgcccgaac ttccagggta
     1561 aggaagtttg tcgctgcttt ctcatcgatc gagatatcgt ccgtgagata gaatcccgcc
     1621 gaatcgacga gttccaccag ggtcttcgag cgctcccgca atgttttaac catcttttcg
     1681 agccacgatt tgtcttgagg aacgggatag cccctggccg caatgtaagg gatcacgtcc
     1741 tctgccagtt gggacaatgg gcgttccttc aggtagtgag agttgaccca gaggaatttc
     1801 tccgggttga atacaccggc tgattttccc acggattcca gggagaactt ttcgatgagc
     1861 tcgtctcgag agaaaatttc ctggtcgcca tgggaccagc cgagtcgaac tagataattt
     1921 aacacggctt cggggaagta gcccatgtcg cggtaagcca tgaccgaggt agcgccgtgt
     1981 cgcttgctca ggcgagcttt gtcggttccg agaatcaacg gcacgtgcgc aaactgcggt
     2041 gggatttggg ccagagcgcg atagaggagc atctgtctgg gcgtattggc gaggtgatcg
     2101 tcaccccgga tgatgtgcgt gatgttcatg tcgatatcgt cgacgacgac gcaaaagtta
     2161 taggtggggg tgccgtctga tcgagcgata atcagatcat ccaactcgcg gttgtcgaat
     2221 ataacttgac ctttgaccgt atcctcgaca atcgtcaagc catcgcgtgg agagcgaaat
     2281 cgaacggtgt atggcttgtc ctttggcaac ggcgggatca ccccctcggg tggtcgacag
     2341 gtgccatcat acatgggctt tcgtttttct ttttgcgcca gctggcgttt ggcatccagt
     2401 tccagggatg tgcaaataca aggatacgct ttaccctcgg ccagaagctg ctgaatcttt
     2461 tccttgtaga gcggatagcg ttcggtctga taaaatggcc cttggtccca atcgagaccg
     2521 agccaattca ttgcttccaa gatagctttg atcgcctcag gtgttgatcg ttcgcggtca
     2581 gtgtcctcga ttcgaagaat gaacttgccg ccgttgtggc gcgcaaaaag atagttgaac
     2641 agggccgttc gtgcaccccc tatatgtaaa tagccagttg gactaggagc gaaacgagtg
     2701 cgaacttgcg ccatttgtgc aaagtaacaa ggagtcgggg tgtggtcaac gacagaagct
     2761 tcgaatgact ctttccgctt acgggctcca taatgacaaa tcgcaacgga gctgttaatc
     2821 tctaataatt ctagggaaca acggtggacg agaccctaaa tccaagcagt gaaaggtctg
     2881 tagtggtacc ggcagaggga acgccgtctc attttattcg aaacatagtt atcgaggatc
     2941 ttcagcaaga caagaacggc ggccgggttc atacgcgatt tccgccggag cctaacggct
     3001 acttgcatat cggtcatgcg aaatccatct gtctcaattt cggtctcgcc gctgaattca
     3061 acggactctg caaccttcgc tttgatgata ccaatccgag caaggaagat gttgaatacg
     3121 tcgaatcgat caaagcggac gtccgttggt tggggttcga ttggggtgat cgagaatact
     3181 tcgcttcgga ttacttcccg cagctctacc agtttgcgat tcagctgatc aaagcgggca
     3241 aggcatacgt ttgtgatttg aacgcagacc agatccgtga atatcgcggc actttaaccg
     3301 agcccggtaa gaatagtccc taccgagaac gatccgttga agagaatctc gacttgttcg
     3361 aacgcatgaa ggcgggagaa tttcccgacg gcgcccgaac cctccgggcg aaaatagaca
     3421 tggcatcgcc caacctcaac atgcgagatc cggtaatgta tcggattctt cacgcgacgc
     3481 atcaccgcac cggcgacgag tggtgtattt atccgactta tgacttcgcg cacggtcagt
     3541 cggattccat agaagggatc acccattcga tttgcacgtt agaattcgaa gatcaccgcc
     3601 cattgtacga ctggtttctc gaacagttgg ctatccacca tccacaacag attgagttcg
     3661 cccgcctcaa tctcacttat acggcactaa gcaaacgaaa gctcttgcaa ctagtgcagc
     3721 aaggtcatgt cagcggatgg gatgatccga gaatgcccac gttggccggc tttcgccgtc
     3781 gcggctatac cccggaagcg attcggatct tttgcgaacg cattggg
//
LOCUS       DASSWZ010000207         7162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_630051, whole genome shotgun sequence.
ACCESSION   DASSWZ010000207 DASSWZ010000000
VERSION     DASSWZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630051"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFN58_05075"
     CDS             <1..897
                     /locus_tag="VFN58_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET8917395.1"
                     /translation="LMNLIVYQGDTEFASVEQQKNLLDTAPTEYDRQALIRVNSEEMR
                     HGWQMCYLLVNYFGDSGKLEARKLLERRAFRGDRLLGSFNAPVNNWLDFFTYTEFVDR
                     DGKYQLTMLSHSSFAPLAESVTAMLKEEFFHMFTGHTGLTRIVRAGTIPLPIMQKYFN
                     KWLSTAYDLFGTDHSSSANWSYVWGLKGRFDEHEAKMPADKNKLNDLARDHYLAECAK
                     LIGQLNQLVPEGQPKLYAPHLKFNRSIGEHVGKTYSVTGELLSPEAYKKHLPEVLPTP
                     DDERILTEIISGKDWVAQTQMN"
     gene            943..1266
                     /locus_tag="VFN58_05080"
     CDS             943..1266
                     /locus_tag="VFN58_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET8917396.1"
                     /translation="MKTLCLVCQNVDCKNRGSEQLMAELQKRVEAKGLADVEVKSYMC
                     FGACQEGPNIVLYPEKSWYASVKVEDLDEITEHLAGGPHVQRLDTIDSSLKELIYQLL
                     DTGVF"
     gene            1270..2526
                     /locus_tag="VFN58_05085"
     CDS             1270..2526
                     /locus_tag="VFN58_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET8917397.1"
                     /translation="MEGILFPRGVVEGREGLKEYQARGGYGALAKAVKSTPEDIIKEV
                     ADAGLRGRGGAGFPTGKKWAFTRECPEQPRYLVLNGGEDEPGSKKDRMLMEHLPHLVI
                     EGVILSAYAIGASKAYLYINANYETATKSITDALAEAKEAGYWGETVSESDFSVAIEI
                     TPAPDNYVAGEDTAALEVIEGKKPWPRQKPPFPVTVGLFGKPTAVNNVETLANVPPIL
                     LHGAEWYRGFGTSESPGTMIFSLNDDVNRPGIYELPFGTPLRYLIEECGGGMKGGKRI
                     KAIMPAAPSSAFLPPDKIDTPLDHNSMREAGSGLGCGVVRLIGEGTCIVEEVLKISEF
                     FTVESCGQCPACRMETNTLTSLLKKVQQGQGGQPILEQFGKILAFNKGKGFCNLIAMP
                     GPPIESALRLFAADFEAHLATGQCPG"
     gene            2672..3685
                     /locus_tag="VFN58_05090"
     CDS             2672..3685
                     /locus_tag="VFN58_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917398.1"
                     /translation="MRVTKIILLAALLSVAQVLNVGAADQDIVVEGAKKEGALVVYTS
                     MTVDQMQKILDAFKTRYPFIRTTMFRAVGERLLTKIMTEAQTGKYDFDVVQSAESQAY
                     FLKKKNLLQKYISPETKNIQKPFFDPEGYWTAVYIMPNVIAYNTRMVKRDEVPKTDED
                     LLNPKWKGKMGMDHTKPEWFAWKLKRMGEEKGLAYMKRLGAQEFQLYAGLSVITNLLV
                     AGEFPLVLNTYLHNVEEVKRKGAPVDWIVQDPVFTKFQPLGIGSKAPHPNAAKLFSDF
                     VLSEEGQKIIASFGRVPTRRGVSTSVQGLDKLNYVIDDISAGDDFNKNFELFRSIFGA
                     PRS"
     gene            3698..4528
                     /locus_tag="VFN58_05095"
     CDS             3698..4528
                     /locus_tag="VFN58_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8917399.1"
                     /translation="MRPKPIKWPNNAKIAVSFFVAFEAFEKYSQYREGGDKPDYASLA
                     YGEYGGKAGIWRIMDVLNRNGVKGTIDTNGRIAEDFPDALKELHATGHEIVGHGWVND
                     TALSSLDAVQEKTLIKRTLDTIASVTGKRPVGWVSPGHRVNEFTFGNFVDEGIFWTGD
                     LPGPDIPQHRIVNGKPLIIMPRLDYANDLSLIFRAKNPARFYFDSFKAAFDCLYAEGQ
                     AGAPKLIDCLVHAHIGGRPNIIGVFEECIRYAKGFPEVWFATKGEIAKYYHDTYVTKS
                     "
     gene            4543..5724
                     /locus_tag="VFN58_05100"
     CDS             4543..5724
                     /locus_tag="VFN58_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8917400.1"
                     /translation="MSTGKIDKKYVEGLKSGELLLKFTWDEWLVVNAPFENVDISVEE
                     VTARYNRNGYDWDTHGTLYTPKTEVNSRRAFVCFHGGAGSEKIMDLTPDGRPGLARVL
                     ASQGFKALALTYPGHYPPGGVWKQPIPERKPIYLFDRDLSDSEILDRNLKCTFNVILQ
                     GAGLLTDNHLAGREIIGFGHSTGGPMAAHLTRFSKKTKIIGLVGFGSGGPDGWRLEWR
                     NKTGAEKYVEKPIDHVSKRSPDSFKASGYVDPEDLTPWGGADDYIRLVSPLRSQMKTS
                     LCDNQHNAAVGILEEYPKRTGLPREEYFDHLQEPDPKWLKTIKVLLTVGENDKGHWVK
                     GDRLQDKREMFMGAKYAATTRGAHVVLIPRYGHVGYAELHNEKIAYLWLWAVKSGYFD
                     G"
     gene            5764..7089
                     /locus_tag="VFN58_05105"
     CDS             5764..7089
                     /locus_tag="VFN58_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET8917401.1"
                     /translation="MVRKIIDQLVDFVLAARDAALPGEVVQKAKNHLLDTLAAIVSGS
                     QLTPGRLGLEYARSEGGKPESLVMGSDLLTTATLAAFANGISAHADESDDSNDRLHPG
                     CAVVPAAWAVAEREKQSGKSLLTAVVLGYEISCRFHKALATKSTTFSGTFGAAVAAGS
                     LLRFDALRNRYLFSYAAQQASGSNAWIADDEHIEKAFDYGGITGRNGVMAALLVQAGF
                     TGNRDVFEGDRNFLQDYPPAEPSQLTSDLGDRYEMTRGLIKKFPVGAPMQEAVEAMHR
                     LIERHKIRAADVVKVVIRLPERAAQTVNNRHMPDVNVQYILAVTLLDGRLGFEAAHDY
                     QRMQRPDVQAMKARVHLEVDLEMDRVGPRYQALVEVSTTSGQQLREHIVNVRGRPENP
                     MSAAEVEEKARALVAPVLGNDRVDRLFDTIRTIEAVTDISSLRPLLLKL"
BASE COUNT         1791 a         1893 c         1935 g         1543 t
ORIGIN      
        1 ttgatgaatt tgatcgtcta ccagggggac acagagttcg catcggtgga acagcaaaag
       61 aacctactcg acacggcgcc cacggaatac gatcgccaag cgcttatcag ggtgaacagt
      121 gaggagatgc gtcacgggtg gcagatgtgc tacttgttgg ttaattattt cggtgattct
      181 ggaaaacttg aagcccgaaa gctcctcgag cgcagggcgt ttaggggcga tcggctgctg
      241 ggctctttca atgcgccggt caataactgg cttgactttt ttacttatac ggaatttgtt
      301 gaccgcgatg gcaaatatca gttaaccatg ctgagccatt cttcttttgc tcccttggcc
      361 gaaagcgtta ccgcaatgct taaggaagag ttcttccaca tgtttaccgg ccacaccggc
      421 ttaacacgaa ttgtacgagc gggaacgatc ccgcttccaa tcatgcaaaa atattttaat
      481 aaatggctat cgacggctta cgacctattc ggtacggatc attcctcctc agcaaactgg
      541 tcctacgttt ggggattgaa gggaagattc gacgagcacg aggccaagat gcccgcggac
      601 aaaaacaaat tgaatgattt agcccgtgat cactatcttg ccgaatgcgc taaacttatc
      661 ggtcagctca accagttggt tcccgaagga caaccaaagc tctatgctcc gcacctgaag
      721 ttcaaccgct ctatcggcga gcatgtgggc aagacctaca gtgtcacggg tgaactattg
      781 tcacccgaag cgtacaagaa gcatctaccg gaagttcttc cgactcctga cgatgaaaga
      841 atattgaccg aaattattag tggaaaagac tgggttgcgc agacccaaat gaattagcca
      901 ccaaggtgat tgattggacc atccgttagc gaggagaggt ttgtgaaaac gctttgtctc
      961 gtctgtcaaa acgtggattg caagaataga ggctcagagc agctcatggc cgaacttcag
     1021 aagcgagttg aagctaaagg gctcgctgat gtcgaagtaa aatcctacat gtgtttcgga
     1081 gcctgccaag agggaccgaa cattgttctt tatcctgaaa agagctggta cgcatcggtg
     1141 aaagtagagg acctcgacga gattacggaa catcttgccg gcgggccgca tgtccaacgc
     1201 ttggacacca tagactcgtc gttgaaagaa cttatttacc aacttctcga caccggagtg
     1261 ttctaaatca tggaaggcat ccttttccct cgcggtgtgg tggagggtcg agagggattg
     1321 aaggaatacc aggcacgagg cggatacggc gccctggcta aagcggtcaa gtccaccccc
     1381 gaagatatca tcaaagaagt tgccgacgcc ggtcttcgag gtcgcggtgg cgcggggttt
     1441 cccaccggaa aaaaatgggc cttcacccga gaatgccctg aacagccacg ttacctggtt
     1501 ctaaatggcg gggaggatga gcccggcagc aaaaaagatc ggatgctcat ggagcatctc
     1561 cctcaccttg tcattgaagg tgtgattctt agcgcctatg cgatcggggc atccaaagca
     1621 tacctttaca ttaatgcgaa ttacgaaacc gcgacaaaaa gtatcaccga tgcgttggct
     1681 gaagccaagg aagcaggcta ctggggtgaa accgtttcag agagcgattt cagcgtcgcc
     1741 atagagatta cccccgctcc tgacaattac gtcgccggcg aagataccgc ggctctcgaa
     1801 gtgatcgaag gaaagaaacc ttggccgcgc cagaagcctc catttccggt tacggtcggg
     1861 ctcttcggca aaccgacggc ggtcaacaac gtggagactc ttgccaatgt gccgccgatc
     1921 cttttgcatg gcgccgagtg gtaccgagga tttggcacat ccgaaagccc tggcacgatg
     1981 atcttctcgc ttaacgacga cgtcaaccgt ccgggaatct acgagctccc cttcggcacg
     2041 ccgttgcggt atttgatcga agaatgtggc ggcggaatga agggcggcaa aaggatcaaa
     2101 gccatcatgc ccgcggcacc gtcttcggct ttcctccccc cagataagat tgacacgccg
     2161 ttggatcaca actccatgcg agaagcgggt tcgggactgg gttgcggtgt cgtgcgtttg
     2221 atcggcgaag gaacctgtat cgtagaagaa gtgttaaaga tctccgaatt cttcaccgtc
     2281 gaatcctgcg gccaatgccc cgcttgccgg atggaaacca acacccttac gtcattgctg
     2341 aagaaagttc agcaaggcca aggcggccaa ccgatcctcg agcaattcgg caagatcctc
     2401 gcttttaaca aagggaaagg cttttgcaat ctcatcgcca tgcccggtcc acccattgaa
     2461 agcgcgctca gactcttcgc cgctgacttt gaagcgcatc ttgcgaccgg ccagtgcccc
     2521 ggctgataac caaaacagac gcaggctatg ctgcgttttc tgcagtgcgc ctcggctgtg
     2581 atagaatccg catcaaactc ttcttccatt tgaccgtctc gacagacgct tgatagtatc
     2641 cgccttgatc aatcaatcga ggtgcagaca catgcgcgtg accaagatca ttctcttagc
     2701 ggcgctttta tccgtggctc aagtcctgaa tgtaggtgct gccgatcagg acatcgtcgt
     2761 tgaaggagca aagaaagaag gcgctttggt cgtctatacc tcgatgaccg tcgatcagat
     2821 gcaaaaaatc ctcgacgcgt tcaaaacccg ctatcccttt attcgaacga ccatgttccg
     2881 cgccgttgga gagagactgc tgaccaagat catgaccgaa gcgcaaaccg ggaaatacga
     2941 tttcgacgtc gttcaatcgg cagaatcgca agcctacttc ctaaagaaga aaaatttgct
     3001 gcaaaaatat atctcacctg aaaccaaaaa catccagaaa ccttttttcg atccggaagg
     3061 ctattggacc gcagtttaca tcatgcccaa cgtcatcgcc tataacaccc gcatggtgaa
     3121 gcgcgacgag gtaccgaaaa ccgacgagga tctgttgaat ccaaagtgga aaggcaagat
     3181 gggcatggac catacaaagc cggagtggtt cgcttggaaa ctaaaacgga tgggagaaga
     3241 gaagggcctg gcttatatga aaaggcttgg cgcgcaggag tttcagctct acgccgggct
     3301 ctcggtgatc accaatctgc tggtcgcggg cgagtttccg ctcgtgctca acacctacct
     3361 gcacaatgtg gaagaggtca aacgcaaagg ggcgccggtc gattggatcg tccaggaccc
     3421 ggtctttacc aaatttcagc ccctggggat cggctctaaa gcgccgcatc ccaatgctgc
     3481 caagctcttc agcgacttcg tgctctccga ggaaggacag aaaatcatcg cctctttcgg
     3541 tagagtcccg acgcggcgcg gcgtgtcgac cagcgtccaa gggctggaca aactaaatta
     3601 cgtcattgac gatattagcg cgggcgatga ctttaacaag aactttgagc tgttccgctc
     3661 gatcttcggc gcgcctcgat catagaaagg acatacgatg agaccgaaac caataaaatg
     3721 gcccaacaac gcgaaaatcg cggtcagctt cttcgtggcc ttcgaagctt ttgaaaaata
     3781 ttcgcagtac cgcgaaggcg gcgacaaacc ggattatgcg tctctcgcct atggcgaata
     3841 cggcggcaag gcgggtatct ggcgcatcat ggatgtgcta aaccgaaacg gcgttaaagg
     3901 cactatcgat accaacggtc gtatcgccga agactttcct gatgcgctga aagaactcca
     3961 cgctacgggt catgaaatcg tcggccacgg ttgggttaac gacacggctc tgtcctccct
     4021 cgatgccgtt caagaaaaaa ctttgatcaa acgaacctta gacaccatcg catcggtcac
     4081 cggcaaacgg ccagtcgggt gggtcagccc cgggcataga gtcaacgaat ttaccttcgg
     4141 caactttgtt gacgaaggaa tattctggac cggagatttg ccggggcccg acatcccgca
     4201 gcaccgcatt gtcaacggca aacccttgat tatcatgccc cggcttgatt acgccaatga
     4261 tctatcgctc atctttagag cgaagaatcc cgcccggttt tattttgaca gcttcaaagc
     4321 agcatttgat tgtctctacg cggagggcca agcgggcgcg ccgaagctca tcgattgtct
     4381 ggtgcacgcg catatcggcg gcagacccaa cattataggg gtgttcgaag aatgcatccg
     4441 ttacgccaaa ggtttccccg aggtatggtt cgcgaccaaa ggggaaatcg ccaaatatta
     4501 ccatgacact tatgtcacta aatcgtaacc ggaggaattg agatgtcgac gggcaaaata
     4561 gacaaaaaat acgtcgaagg actgaaatct ggcgagctgc tcttgaagtt cacctgggat
     4621 gagtggctgg tggtgaacgc accgtttgag aacgtagaca tttcagtcga agaggtcacg
     4681 gcgcgctaca acagaaacgg ctacgattgg gacacgcatg gcacgctcta taccccaaag
     4741 acagaagtta attcccgtcg ggccttcgtt tgtttccacg gcggagcagg cagcgaaaag
     4801 atcatggacc tcacgccaga cggcagacca ggtttagccc gagttctagc ttcccagggc
     4861 ttcaaagctc tcgctctcac ctatccaggc cactatcctc ccggaggcgt ttggaaacag
     4921 ccgatccccg agcgcaaacc gatttacctg tttgaccgcg acttatcgga cagtgaaatt
     4981 ctcgatcgca atctaaaatg cacattcaac gtcattctcc aaggcgcggg gctactaacg
     5041 gacaatcacc tcgccggacg agagatcatc ggcttcggcc actccaccgg tggaccgatg
     5101 gctgcgcacc tgacgcgctt ttccaagaaa acaaaaatca ttggacttgt gggtttcggc
     5161 agcggtggtc cagatggctg gagactcgaa tggagaaaca aaaccggcgc ggaaaagtat
     5221 gtcgaaaagc ccatcgatca tgtttccaag cggtcgccgg attcattcaa agcatcaggc
     5281 tatgtcgatc cggaagattt aacaccttgg ggcggcgccg atgactacat ccgtctggtc
     5341 agcccgttgc gctcgcagat gaagacaagc ctgtgcgaca accagcacaa cgcagccgtg
     5401 ggaattctcg aagaatatcc caagcgcaca ggcctcccgc gggaggaata ctttgatcac
     5461 ttgcaagagc ccgatccgaa gtggctcaaa accatcaaag ttcttctcac cgtgggtgag
     5521 aacgacaaag gtcactgggt gaaaggcgat cggttgcagg ataagcggga aatgttcatg
     5581 ggcgctaagt acgcggctac tactcgcggc gcgcatgtag ttttgattcc gcgttacggc
     5641 catgtgggct atgccgaatt gcacaacgaa aaaattgctt acctatggct atgggcggtt
     5701 aagtcaggct acttcgacgg ctaactttgt ttcctctgct ggtaaaagga acggaaaaac
     5761 accatggtca gaaagatcat cgaccagctt gtagacttcg tgttggccgc gcgcgacgct
     5821 gcgctgcccg gcgaggtcgt tcagaaagcg aaaaaccacc ttttagacac tctggcggca
     5881 atcgtttccg gttcgcagct cactcccgga cggctaggct tggaatatgc ccgctcagaa
     5941 ggtggtaaac cggaatcgct ggtgatgggt tccgatctgc tgacaacggc gactctagcc
     6001 gcctttgcca atggtatttc tgctcacgcc gatgagagcg acgattccaa tgaccgcttg
     6061 catcccgggt gcgccgttgt tcctgcagcg tgggccgtcg ccgagcggga aaagcaatcg
     6121 ggcaaatctc tactcaccgc cgttgtcctt ggctatgaga tcagctgtcg ctttcacaaa
     6181 gcgttggcga ccaaatcgac gactttcagt ggcacatttg gcgccgccgt agccgccgga
     6241 agtctgcttc ggtttgatgc gctgcgaaac cgctatcttt tttcttacgc cgcccaacaa
     6301 gcttccggat cgaatgcctg gatagccgac gacgagcata tcgaaaaggc cttcgactat
     6361 ggtggaataa ccggccgcaa cggcgtcatg gccgctctcc ttgtgcaagc tggattcacg
     6421 ggcaatcgcg atgtgtttga gggcgatcga aactttttgc aagactatcc accagcggaa
     6481 ccgagccagc tcaccagtga tctcggtgat cgctatgaga tgacgagggg attgatcaag
     6541 aagtttccgg tcggcgcgcc catgcaagag gcggtggagg caatgcatcg tctaatcgaa
     6601 cggcacaaga ttagagccgc cgatgtcgtc aaggttgtta tccgcttgcc ggaaagagct
     6661 gcccagacgg tcaacaatcg ccatatgccc gacgtcaatg ttcagtacat cctggccgtg
     6721 actctgctgg acgggcgctt aggctttgag gcggcgcatg attaccagcg gatgcaacgc
     6781 cccgatgtcc aagccatgaa agcgcgcgtc cacttagagg tagacctaga gatggacaga
     6841 gttggacccc gctatcaggc tctggttgaa gtgagcacta ccagcggcca gcagttgcgc
     6901 gagcatatag tcaatgtccg cgggcgacca gagaatccga tgtccgcggc ggaggtggag
     6961 gagaaagcgc gcgcgctcgt ggccccggtg ctgggcaatg accgggtcga tcggttgttt
     7021 gatactatta ggaccatcga ggcggttacc gacatctcca gtttgcgtcc gcttttgctg
     7081 aagctttgat gcctatggcc ctcggaatga cacgtttaag gcagcgagct attctgataa
     7141 gtgtctcttc gcgaaggagg aa
//
LOCUS       DASSWZ010000208         5211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_149037, whole genome shotgun sequence.
ACCESSION   DASSWZ010000208 DASSWZ010000000
VERSION     DASSWZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149037"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..766
                     /locus_tag="VFN58_05110"
     CDS             137..766
                     /locus_tag="VFN58_05110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET8917402.1"
                     /translation="MRLLLASTSPRRREILSLLGLPFEVIAPQFDELPLSDRPIREEV
                     LDFAVGKALSVASNQPGTMVVGSDTMILINATKIGKPAGIADAKQMLRLLSGKTHRIF
                     TSVAIIDDLGGPGLRMVEEVAVKMHDYSMKEIEHYLSCNESLDKAGAYSIQGQGRSLI
                     ESIRGDYLAAVGLPLKPIADYLKSCGISVPSDVDKLYSDKSFMNWKSFS"
     gene            1496..1909
                     /locus_tag="VFN58_05115"
     CDS             1496..1909
                     /locus_tag="VFN58_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HET8917403.1"
                     /translation="MEISDAQTFLKDNHHGVLVARKKDGSLQMTLVSPVIDAEGKVII
                     TARDSTYKVKNIKRDPRISLLVYGDKFNGSDYIQIDGKAEVIEHPRAMDIVLDWHRKI
                     RGVPDNWDDIRKKTLSEGRIAIRVTIENAGPQSRG"
     gene            2712..4391
                     /locus_tag="VFN58_05120"
     CDS             2712..4391
                     /locus_tag="VFN58_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8917404.1"
                     /translation="MSRITGADLICRALKLEGVSNIFALAGDHILPVLDTMADQEFHI
                     IDTRHEQAAVHMADAWARITEQPGVCMYTTPGFANAIPGLTNALHSESPVISISGCAD
                     LHDLGRGGQQEIDQMHMAAPMTKGSFLVDDARRIPEFIRRAMRLAFSGRRGPVHLTIP
                     IDLQEGSVDEEQVALYEPDEYRAKEAILAHPESVGRAIALLRQAKKPLIIAGSAAGYT
                     LSGDALQRFIETTRLPVLTEEQSRGLISDDHPYVFGFFERGLNRAAAKVREADVVLLL
                     GRKQDFTIGFCRPPHVAADAKIVQVDPSALEIGRNRGVAVGMVGDVTSVLNQMTKEAA
                     SFTWKDLPWLDELREARAAQLVWTENLARTETPMHALFVHKTIKSMLRPDDCLVFDGG
                     DFCHFGRSLLPARKPKRWFYVSGLGMLGSSLPTALAAKVAYPDSRVIMLTGDGAFGFN
                     GMEFDTAVRHRLNIVAILGNDSAWGIDRQIQIGLYGRPVATELLQTRYEQVVQGLGGY
                     GEFVERAEDLEPALQRAFASERPALLNVAVQRAISPRAEAAIGRRRAANAK"
     gene            4604..4735
                     /locus_tag="VFN58_05125"
     CDS             4604..4735
                     /locus_tag="VFN58_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917405.1"
                     /translation="MMIHKLFVSMTVAGLLCGYMAMLYALMRIVVLIDRSYFLSLAM"
     gene            complement(4762..>5211)
                     /locus_tag="VFN58_05130"
     CDS             complement(4762..>5211)
                     /locus_tag="VFN58_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET8917406.1"
                     /translation="GAQTAKLEKAINSSPKSEETRLIQVRLKDTGFDPGPIDGVLGAK
                     TRAAFLRFEAGCTMLNNLPSTLDKELQIADRRKFVPSEEKSYPVAAKMMGIESHKMTL
                     SSEKIRQEQLRLRDAGFDPGPIDGILGPKTKVAMQRYQKSFALKNSQ"
BASE COUNT         1153 a         1366 c         1371 g         1321 t
ORIGIN      
        1 cccctggtgc ggcggagctg cgcgcgcaga tggttcgcgt gagtcacatc gaagaagttg
       61 tccagtgcct cagggcctat gccgggacgc tccgcgagca cgattcacca gccataagca
      121 cggctttttt gttttcatga ggctcctgct ggcgtccacg tcgccccgcc gtcgtgagat
      181 cttgtcgctg ctcggcttgc ccttcgaagt catcgctccg cagtttgatg aactgccgtt
      241 aagcgatcga cccatcagag aggaggttct cgacttcgcc gtaggaaaag ccctatcggt
      301 tgccagcaac caaccgggaa ccatggtcgt gggcagcgac actatgattc tcataaatgc
      361 taccaagatc ggcaaacctg ccggaatcgc tgacgcgaaa caaatgctgc gcttgctttc
      421 gggaaagacc catcggattt tcaccagcgt cgccatcatc gatgatttgg gcgggccggg
      481 tctcaggatg gtcgaagaag tcgcggtaaa gatgcacgac tactcgatga aagagatcga
      541 gcactatctc tcctgtaacg aatcgctgga taaagcgggc gcttattcca tccaaggtca
      601 agggcgtagt ttgatcgaat ccattcgcgg tgattatctg gccgccgtag gcctgcctct
      661 caagccgatc gccgactatt taaaaagctg cggcatctcc gttccgtcag atgtcgacaa
      721 gctctactct gataagtcat tcatgaattg gaaaagtttt tcataaactt tgcgtttgtg
      781 gcaagaggaa cgacgcattt tcctagaaca agaaaacgat tgcgtcccac actctctttt
      841 gttcctattt ctcccgacca aggtatagct tttcgccctt cgatcacggt tccttcacga
      901 gcgcgtctat ctcttcgcgg atgtgtgttc tttgcggttg aacgttcaaa atttggcggt
      961 aagtaacgat tttcgttcag gcatgccagt gacttctgaa aaattgttgg aatttcacat
     1021 aggagatgtg gcatggtgat tgctgagcca tcctcagatc gtcatgaaac cgccggagtt
     1081 cgaccccaac tctgatcgta actgggcctg gcccgaaaaa acgtggttcc gcgagcggct
     1141 gaatgcgttg agggcgcggg agcacgaaac tcgcaagccc gccggcaacc agaaacaatc
     1201 ggtggctatg gagtcgaaac ctagtggcga ctcaggaaca gaaaaagctt aactaggctc
     1261 cgacccacgc aggtaaaagt tccagtattc aggacccgct gttttgatcg tcaactcgct
     1321 tcggttaaaa aacttctgcc ttggctccaa gtagaacgga ccgtcaaacc acgtggaccg
     1381 ttaggaaccg tccagcggac agctctcctt ttatcttcac aagtacaatc acacgactgg
     1441 tccttgaata gttgcctccg tatcgcgtag tatccgttgt cccattggag gagttatgga
     1501 aatttctgac gcacaaacat ttttaaaaga taatcaccat ggcgtcctcg tcgcccggaa
     1561 gaaagacggg tcgttgcaga tgaccctggt ttcgccggtg atagatgccg aagggaaggt
     1621 aatcattact gcgcgtgatt cgacctataa ggtcaagaat ataaaacgcg acccacggat
     1681 ctctttattg gtttacgggg acaagtttaa cggctctgat tacattcaga tcgacggcaa
     1741 agccgaggtc atcgaacacc cccgggccat ggatattgtc ctcgactggc accggaagat
     1801 tcgtggtgtt cccgacaatt gggatgatat tcggaagaaa acgttatccg aaggccgtat
     1861 tgctatccgt gtcaccatcg aaaatgccgg cccgcagagt agaggataag cataacgacc
     1921 tcacggcgct gcccccgtcc cctaagcgat ccccgcaacc cctgttgttt ttgagttcac
     1981 tgcttgtctt cctcgtttcc ttgagagttc ggcaaagttt gtttcggttt tgcactgatt
     2041 gtccaagtct gagcattgtt cgatagaaat ctacgtttct accgaagaat ttgtggcgtt
     2101 ttttcacggc attcatcttg ctccgctcat tcgcctggcc ccgactaaag aacagctcga
     2161 tagaagctct tgcaaggggt tcttatgatt ggcgcgtgaa cggtcaatgg gtaccttcga
     2221 tatagtactg ctagcgacct tctggtacat gctgaaatct gtgcccggac tcatgcaatt
     2281 cgttggctac tttttcggct ttgtattagc gtcgtgggct tttgatcaat acttccagcg
     2341 cgactgaaac acgaaaacgc tgctcttcaa ttttccaact tcaactgttg aatcctccta
     2401 acaaccgggg gcaggctggc tgtgtccaag ggtccgccgc gctacaccga taaatcccgg
     2461 cagattcgat taagtactgc ggcaggagat gcccccagct ctccaaagaa gttctcctgt
     2521 atcgtgtcga tttttggact gtttctgttg cctgtagtgc tttgatgatt gttcggacga
     2581 cgattttgat caaaaccgga ggaaacactt gactgcattt gcgcacttgt atgatacagg
     2641 ggataacgca attggaacta gaggaaaagt tagcctgacg ctttttcgtc agcaaaccta
     2701 aaggagcgat aatgagtcga atcactggtg ctgatctaat ctgcagggcg ctcaagcttg
     2761 aaggggtgag caatattttt gccttggccg gtgatcatat cctgccggtg ttagacacga
     2821 tggcggatca agagttccac atcatcgata cccggcacga acaggcggcg gttcatatgg
     2881 cggatgcttg ggctcgcatc acggagcagc ccggcgtttg catgtacact actccggggt
     2941 ttgccaatgc aattcccggc ctcaccaacg ccttgcacag tgaaagccct gtcatttcta
     3001 tctcaggttg tgccgatctc cacgacctag ggcgtggtgg ccagcaggag atcgaccaga
     3061 tgcatatggc tgcgccgatg accaaaggtt cattcctcgt ggatgacgcg cgaaggattc
     3121 ctgaatttat tcgccgagca atgcgcctgg ctttcagcgg ccggcgcggc ccggttcact
     3181 tgacgatccc cattgatcta caagaaggaa gcgtagacga agagcaagtg gcgttgtacg
     3241 aaccagatga ataccgcgcg aaagaagcga tccttgccca tccggaaagc gtaggtcggg
     3301 cgatcgcgct cctgcggcag gcaaagaaac ctctgattat tgctggaagc gccgctggtt
     3361 atactctttc cggtgatgcc ctgcagcgct tcattgaaac tacccgcctg ccggtgctga
     3421 cggaagaaca atcgcgcggt ttgatctctg acgatcaccc atacgtcttt ggatttttcg
     3481 agcgcggctt gaatcgagcc gccgccaagg ttcgcgaagc cgacgttgtt ttgctgctgg
     3541 gacgcaagca agactttacc atcggtttct gccgtccgcc ccatgtggca gcggatgcca
     3601 agatcgtcca ggtcgatcct tcggctttgg agatcggtcg aaatcgggga gttgccgtgg
     3661 gcatggtcgg cgatgttacc agcgttttga atcagatgac gaaggaagcc gccagtttca
     3721 catggaaaga ccttccgtgg cttgatgaat tgcgcgaggc gcgcgctgcg cagttggtat
     3781 ggacggagaa tttagcgcgc accgaaaccc cgatgcatgc cctgtttgtt cacaagacca
     3841 tcaagtctat gctgcgtccc gacgactgcc tcgtctttga tggcggagac ttttgccatt
     3901 tcggtcgttc tctattgcct gcccgcaagc ccaagcggtg gttttacgtt tccggtttag
     3961 ggatgttggg atcgtcgcta cccacggcgc ttgcagctaa ggtagcgtat cccgactcac
     4021 gggtgattat gctcaccggc gatggagcgt ttggcttcaa tggcatggaa ttcgataccg
     4081 cggtgcgcca taggctcaat atcgtggcga tattaggtaa cgattccgcc tggggtatcg
     4141 accggcagat ccagatcggc ttatacggcc ggcccgtggc taccgagttg ctgcagacgc
     4201 gctacgaaca ggtcgttcaa ggccttggtg ggtacgggga gtttgttgag cgtgcggaag
     4261 atctggaacc cgcgttgcag cgcgcgtttg cttcggaacg gccggcgctg ctcaacgttg
     4321 ctgttcagcg ggcgatcagt cctcgagccg aagccgctat cggtcgccgg agagctgcca
     4381 atgccaagtg attcagcctt tcagacttga ctgaatcatt tcggatactc acccgtatca
     4441 taaagttact tgctctttgg tctccaaagc catgccaagc cggccgtcct aaaattccat
     4501 tggtattccg ctaggttgct caaacgtccc cgtagtgact ccgactggca tcaacattgc
     4561 gctagtagtt tgacgacaac tgaggaaacc aatggaggga tttatgatga tacacaagct
     4621 gtttgtctcg atgacggttg ctggacttct ctgtggatat atggcgatgc tctacgcgct
     4681 catgcgcatc gtagtcctta tcgatcgttc ttatttccta agtctggcca tgtagaggct
     4741 tgggagcggg tgtgtcgcta gctattgtga atttttcagt gcaaatgact tctgataacg
     4801 ctgcatggct actttagttt taggacctag gatcccatcg atcggacccg ggtcgaaacc
     4861 ggcgtctctc aatcgcaact gctcttgccg gatcttttca ctgctcagag tcatcttatg
     4921 ggactcaatc cccatcattt ttgctgccac cggatagctt ttctcctccg aaggcacaaa
     4981 ttttcgtcta tcagcaattt gaagctcctt atccaaagtt gacggcaaat tattgagcat
     5041 cgtgcatccg gcttcgaaac gtaaaaacgc cgctctcgtt ttggcgccca aaaccccatc
     5101 gattggaccc ggatcgaaac cggtatcttt cagtcgtact tgaatcaggc gggtctcttc
     5161 gctcttcgga ctgctattaa tcgccttttc cagttttgct gtttgagcac c
//
LOCUS       DASSWZ010000209         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1167733, whole genome shotgun sequence.
ACCESSION   DASSWZ010000209 DASSWZ010000000
VERSION     DASSWZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167733"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VFN58_05135"
     CDS             complement(<1..1202)
                     /locus_tag="VFN58_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HET8917407.1"
                     /translation="MRYELFIGLRYLRARRRETFISLITIISVLGVMIGVMTLNVVMA
                     VMTGFEETLRDRLLGINAHVALVKSGDQLREYEKLLELVRQTKGVVAASPSIYGQVML
                     TSGPRVSGVVVRGVDPDKVNQVVDIERYIREGSLQSLKQQQPLQVQGRTILLPGVIIG
                     TRLANQLGVFLGSPIQVVSPLGSPTAIGVIPKVRRFVVVGIFDSGMSEIDSTLVYMNL
                     KDAQTFFELGDAVTNIEIRVQDVYRAQSVAQDIQRRLGFPYLTEDWSRLWPNLFSALK
                     LEKTVYFLVLLLMVLIGAFNIISTLTMVVMEKRKDIAILQSMGATRESIRRIFLIKGC
                     VIGVVGTLLGVFLGLAICLLIQKYRFIELPKDVFLISTVPVRIYLSNFLLVGFASLFV
                     CLLASIYPA"
     gene            complement(1223..>2157)
                     /gene="lysS"
                     /locus_tag="VFN58_05140"
     CDS             complement(1223..>2157)
                     /gene="lysS"
                     /locus_tag="VFN58_05140"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET8917408.1"
                     /translation="RRFFEERDFLEVETPMMQAVPGGAAARPFVTHHNALSMDLYLRV
                     APELFLKRLLVGGFERVFELNRNFRNEGVSVRHNPEFTMLEFYQAYATYDDLMRLTEE
                     LFVTLAEEVVGSLQVPYADHLIDLKPPWRRLTIKEAVVQYGGAQEQEIGTLEGLQRFA
                     KQEGLHVDLDVSYGNLLVEVFEQVAESQLIQPTFMTGHPLEVSPLARKNDANPALVDR
                     FELYVGGRELANAFSELNDPADQRQRFLQQVEARNAGDETANPIDDDYVRALEYGMPP
                     AAGEGIGIDRLVMFFTNSPSIRDVILFPLLRPQR"
BASE COUNT          567 a          598 c          531 g          461 t
ORIGIN      
        1 gccggataga tgctggcgag aaggcataca aaaagcgaag caaaaccgac gagcaagaaa
       61 ttactcaagt agattctcac cggcacggta gagatgagaa agacatcttt gggaagctca
      121 atgaaacgat atttttgaat caacaggcaa atggccaatc ccaaaaacac gccgagaaga
      181 gtccccacga caccgatcac gcagcccttg attaggaata tccttcgaat actctctcga
      241 gtcgcaccca tggactgaag aatggcaata tccttcctct tttccattac aaccatggtc
      301 aatgtggata taatattgaa tgcacctatc aggaccatta ggagcaagac gagaaagtaa
      361 accgtttttt ccagtttcag ggcagaaaac agattgggcc aaagacggga ccaatcttcg
      421 gtcaggtagg gaaatcccag tcggcgttga atatcttgcg ccacgctctg ggcgcggtag
      481 acatcttgca cccgaatttc aatattcgtg accgcgtctc caagctcaaa gaacgtctgt
      541 gcgtctttca aattcatgta aaccaaggtt gagtcaatct cgctcattcc ggaatcaaaa
      601 atcccaacaa caacgaaccg gcgaactttc gggatgacgc cgatggcggt tgggctcccg
      661 agaggagaga ccacttgaat cgggctgcca agaaaaacac cgagctgatt tgccagcctg
      721 gtccctatga tcacccccgg caacagaatc gtccgaccct ggacttgaag gggttgttgt
      781 tgcttgaggc tttgaagact gccttctcta atgtaacgtt cgatatccac gacttgattg
      841 actttatcgg gatcaacgcc gcgaacgact accccggata ctcgcggtcc ggaagtcaac
      901 atgacctgtc cataaatgga cggcgacgct gcaaccaccc cttttgtttg ccgtaccagc
      961 tcaagaagtt tctcgtattc cctgagttgg tcaccggatt taaccagcgc cacgtgtgca
     1021 ttgattccaa gcaaccggtc ccgcaacgtt tcttcaaaac ccgtcatgac cgccatgaca
     1081 acgttcaaag tcattacgcc gatcatgaca cccagcaccg aaatgatagt aatgagagaa
     1141 atgaaggttt cacggcggcg ggcgcgcaag tatctaaggc cgatgaagag ttcgtatcgc
     1201 atcagggagt ctcgctgtgg tctcagcgtt gaggccggag cagcggaaaa agaataacat
     1261 ccctaatgga gggggagttc gtaaagaaca tgaccagccg atcaatacca atcccttcac
     1321 cggctgcggg cggcattcca tattcgagag cccgaacata atcgtcatcg atggggttgg
     1381 cggtctcgtc gccggcgttt ctcgcctcca cttgctggag gaatcgctga cgttgatctg
     1441 ccggatcgtt caactctgaa aaagcattgg ccaactctcg acctccgaca tacaactcaa
     1501 aacggtccac caaggccggg ttcgcgtcgt tttttcgcgc cagcggggaa acctccaacg
     1561 ggtgcccggt catgaacgtc ggctggatca actgagactc cgcgacctgc tcaaagacct
     1621 cgactaggag attgccatag gagacgtcga gatcgacgtg taggccttcc tgcttggcga
     1681 agcgttgcaa gccctcaagt gtgccaattt cctgttcctg tgccccgcca tactgcacta
     1741 ccgcttcctt gatcgtaagc ctccgccacg gcggtttgag atcgataagg tgatctgcat
     1801 agggcacctg cagcgagcca acgacttcct cggcaagggt aacaaaaagc tcttctgtga
     1861 gacgcattaa atcgtcgtaa gtggcatagg cctgatagaa ctccaacatg gtgaattccg
     1921 ggttatggcg caccgagacc ccttcgttgc gaaagtttcg attcagctca aatactcttt
     1981 cgaatcctcc caccaaaagg cgcttcaaaa acagctcggg cgccacgcgc agatacagat
     2041 ccatactcag ggcgttgtga tgcgtaacaa aaggacgcgc cgccgctccc ccgggcaccg
     2101 cctgcatcat cggcgtttcc acttccaaga aatcccgctc ctcgaaaaag cgccgaa
//
LOCUS       DASSWZ010000210         4391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1252805, whole genome shotgun sequence.
ACCESSION   DASSWZ010000210 DASSWZ010000000
VERSION     DASSWZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252805"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3039)
                     /locus_tag="VFN58_05145"
     CDS             complement(<1..3039)
                     /locus_tag="VFN58_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET8917409.1"
                     /translation="MTSKPVSSELTQAPSFTSRLFFFLDKKRLKIWLWVTLSVLLVVA
                     CVYIEIQTSLLQSWFFTRTNEQLSYQLKEGQSTAIAFPRSAPFDDRRGYSKLPAFQSR
                     LEGQGYQVAQQIHQSEKMVQLIKRGISPPYVEPPETGLDIRGANGAPLFRHAQSEFLF
                     AKMDDIPPLLVKTLLFLENRDLDHPASPWQNPAVEWDRLFKATFFYIASKLYIEVPVQ
                     GGSTLAVQLEKFRHSPKGRTESPVEKYRQLVGASLKAYREGWNTSAWRERIIVDYFNT
                     VPLAAAPSYGEIHGLGEGLFAWFGMQLTDVVSALKAPGLSPAKVRAFKHALALLISVR
                     APTVFLVDERASLEEKVDQFTRLMARSGIIDEDFAAALQETPIKFIPAAPLPPQPSSG
                     KNKAANAIRTTMMESLGTNNVYDLNRFHLEVQSTIDVPLQKTITEFLHSLANPDVIKA
                     RGLNGERLLENADPKKVIYSFLLVEPTREGNLVRVQADNLAAPFDFNKSVKLELGSTA
                     KLRTLTHYLELIAALHKELAALDADGLKAKAQEARDPLTKWAVETLQKEKNLTLEAFL
                     DRAMERKYSASPWEPFFTGGGIHHFENFEREENKRILELRDAFRNSTNLVFIRLMRDI
                     VSYHRARLQYNSDAVLSDPTNPDRKRMLQEIAEEESRTVLRRSYQAYHGQTEQQIVAR
                     LLETKKNAERRLAVLFFAWRIGTDEQALTAWLEQNQIKIKEQDVSKLFRAYQNPRLTL
                     VDYAYLLSLHPLDLWCASEYRRNPELSWEKLVADSSDARRQGSAWLLNPRNRRAQDLR
                     LRIIMEKDAFTHMVPYWQRLGFPFKTMVPSYATAIGNSSDRPVALAELIGILVNDGVR
                     RPNTELSNIHFARGTPYETVFERKTEPGQQVLVPEVARAVRKAMAGVVESGTARRLAG
                     VFRLSDGKLITVGGKTGSGDNRIKTFNRWGNVTSSRATNRTAAFVFYIGDRYFGVITA
                     YVQGREAEHYHFTSSLPVTLLKLLAPTIIAKLDKNVVQPPP"
     gene            3329..>4391
                     /locus_tag="VFN58_05150"
     CDS             3329..>4391
                     /locus_tag="VFN58_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HET8917410.1"
                     /translation="MLATRIVQKEGTFYFIAYKAADLLDRVSFTSRYYFEGEQIEADE
                     PGEDEVARFIAGIEKSEKSFQRILNRRKIRQIVNFYETAVSQPLIPGTVLLFTDETLR
                     FRRSDVSESVGFLSEPDSKFLVIDGQHRLAALHFFRQKHPELAEQVEVPCVVFDGKSA
                     DFATEMFVIINSTHTRINKSHLVDLYERVSWETPEKKFAAKVVNLLYEESDSPLRYRI
                     NRLGGRSRQEKWILQSELFNEIHKVVQSNRRFFEERFQLRADRAYGLIRDYLKAVREV
                     MNSIWGDNERYMFTRDVTLKALLRVFGDLIRDRKLVQVWEESRDPRAFAGAMRPWAEL
                     TREFRAEGFYERFPAKGQVE"
BASE COUNT          986 a         1149 c         1261 g          995 t
ORIGIN      
        1 gggaggtggt tggacgacat tcttatccag cttcgcgatg atggtcggcg ccagtaactt
       61 gagaagggtt acaggcaggg agctggtgaa atggtagtgc tcagcttcac gcccttgcac
      121 atacgcggtg atgacaccaa agtagcgatc accgatgtag aaaacgaaag cggcggttct
      181 attggtcgcc cgggaagagg ttacgttccc ccatcgattg aaggtcttaa ttcggttgtc
      241 accggaaccg gtttttcctc cgaccgtgat cagtttcccg tcggacagcc gaaaaacacc
      301 agcgagtcgt cgcgcggttc cgctctccac caccccggcc atcgcctttc tgaccgcccg
      361 agccacctcc gggacaagca cttgctgtcc tggctccgtc ttccgctcga agacagtctc
      421 gtagggcgta ccgcgggcga aatgaatgtt gctcagctcc gtattgggcc gccgcacgcc
      481 gtcgttgacc aagatcccga ttaactcagc caaagccacg ggtcgatctg aggagttgcc
      541 gatcgcagtt gcgtaggaag gcaccatagt tttgaacgga aagcccagcc gttgccagta
      601 gggcaccatg tgggtgaagg cgtccttctc catgatgata cgcaggcgga ggtcctgggc
      661 ccttcggttg cgcgggttga gcaaccatgc agaaccctgg cgccgggcat cgctgctgtc
      721 ggcgacgagt ttctcccatg acagctcggg atttctccga tattcgctgg cacaccagag
      781 atccagcggg tggagcgaaa gaagataagc atagtccacc agggtcagac gagggttttg
      841 gtaagcacgg aacagcttgg agacgtcctg ttccttaatt tttatttggt tctgttccag
      901 ccaggccgtc aatgcctgct catcagtgcc gattcgccag gcgaaaaaga gcaccgccag
      961 tcgccgctca gcgttctttt tcgtctccaa cagtcgggcg acgatctgtt gttccgtctg
     1021 gccgtgatac gcctgatagg agcggcgcaa aaccgtgcgg gattcttctt cggcaatctc
     1081 ctggagcatg cgtttgcggt ccggatttgt cggatcggaa aggacggcat cggagttata
     1141 ctgcaagcgc gcgcgatgat agctcacgat gtcgcgcatc aaacggatga atactaggtt
     1201 cgtcgagttt cggaacgcat cccggagttc aaggatgcgc ttattttctt ctcgttcaaa
     1261 gttttcgaaa tggtgaatgc cgccgccggt aaagaagggt tcccaggggc tcgctgaata
     1321 tttgcgctcc atagcgcgat caagaaaggc ttccagggtg agattctttt ccttctgcaa
     1381 ggtctcaacg gcccacttgg tcaagggatc gcgcgcttct tgagctttcg ccttcaagcc
     1441 atcggcatcc agcgccgcaa gttctttatg cagcgccgcg atgagctcaa gatagtgtgt
     1501 cagcgtacgc agtttggcgg tactgccgag ttcgagtttg acgctcttgt tgaaatcgaa
     1561 cggggcggcg agattatccg cctgcacacg cacgaggttt ccttcccggg taggctccac
     1621 caataggaag gagtagatta ctttcttagg atcggcgttc tcgagaagcc gctcaccgtt
     1681 caatccccgg gctttgatga catccggatt cgccaaacta tgaaggaatt cggttatcgt
     1741 tttttgcagt ggaacatcga tggtgctttg gacttccaaa tggaaacgat tcagatcgta
     1801 gacattgttg gtaccaagag attccatcat ggtcgtgcga atagcgttgg ccgctttgtt
     1861 tttgcccgac gaaggttggg gcggtagcgg cgccgcggga atgaacttga tcggggtctc
     1921 ctgcaatgcc gcggcgaaat cctcatcgat gattcctgat cgcgccatca accgggtaaa
     1981 ctggtccacc ttttcttcca gcgaagcccg ttcatcaacc aggaaaacgg ttggtgcgcg
     2041 cactgaaata agcaatgcca gggcatgctt gaaagcacgc actttggcgg gactcagtcc
     2101 cggagccttc agcgcgctga caacgtccgt caactgcatt ccgaaccaag cgaaaagccc
     2161 ttcgcccaga ccgtgaatct cgccgtagct cggcgccgcc gcgagcggaa cggtattgaa
     2221 ataatcgaca atgatccgtt ctctccacgc gctagtattc cacccttcgc ggtaagcctt
     2281 gaggcttgcg ccaacaagct ggcggtattt ttcaacgggt gattcagtgc gccctttggg
     2341 cgaatggcga aacttctcca attgcacggc gagggtgctc cctccctgta ccggcacttc
     2401 gatgtaaagc ttggaagcga tatagaaaaa ggtggccttg aataagcggt cccactcgac
     2461 ggcgggattc tgccacgggg aagcggggtg gtcgagatcc cggttttcga gaaaaagcag
     2521 cgttttcacc agcaggggtg gaatgtcgtc catcttggcg aacaaaaatt ccgattgagc
     2581 atggcgaaac agtggcgccc cgttggcgcc ccggatatct agtccggttt caggcggttc
     2641 cacgtaagga ggcgagattc ctcgcttaat gagctgcacc atcttctccg attggtgaat
     2701 ctgctgcgcg acctgatatc cctgaccttc aaggcgggac tgaaaggcgg ggagctttga
     2761 atagccccga cgatcatcga aaggagcaga acgaggaaaa gcgattgcag tactctggcc
     2821 ctctttcaat tgataggaaa gttgctcatt ggtgcgagta aagaaccaag attgcaagag
     2881 cgaggtttga atctcaatgt aaacgcaagc aaccacaagc aacacggaca gagtcaccca
     2941 aagccaaatt ttgagtcgct tcttatcgag aaaaaagaag aggcgggagg tgaatgaagg
     3001 cgcctgtgtc agttcggagg aaaccggttt actggtcatc ggggtatccg aataaagata
     3061 ccataaacag aggtaatttg cgcgtggggg cttttggcga tgaatatacc cttaaacggt
     3121 cgcacgggtc tattggacct tagtccaata ggggcatctc ttgcccagtt gctcctttgc
     3181 ggcgactccc ctgggtcagg ctcgacccga acccccttcg cctcaaggat ggacgctgag
     3241 aaggcgtcta tttagcggtt tctggaggtt cttttcagtg gcaaaaggcc aggcgaggga
     3301 tttataatcg gccggagggg gtggtcttat gctggcgaca cgcattgttc aaaaggaagg
     3361 caccttttat ttcatcgcgt acaaggcggc agatcttttg gatcgagtca gtttcaccag
     3421 ccgttactat tttgaaggcg aacagatcga agcggacgag cccggcgaag acgaagtcgc
     3481 ccggttcatt gctggcatcg aaaaaagcga aaaatcgttc cagcgtatcc ttaaccggag
     3541 aaaaatccgc cagatcgtca acttctatga gaccgcagta agccagccct tgattccggg
     3601 tacggtcctt ctgtttaccg acgagacctt gcgctttcgc cgctcggatg tctccgaaag
     3661 cgtcggcttt ttaagcgagc cggacagcaa gtttctcgtt atcgacggcc aacaccggct
     3721 ggcggcgctc catttctttc gccagaagca cccggagctg gcggagcaag tggaggttcc
     3781 ctgcgtggtc ttcgatggca agagcgccga ttttgccact gagatgttcg tcatcatcaa
     3841 ctcgacccac acccggatca acaaatcgca cttagtggat ctttacgagc gggtgtcctg
     3901 ggaaacgccg gagaaaaaat tcgccgcgaa ggtcgtcaat ctactgtatg aggaaagtga
     3961 ttcaccgctg cgctatcgca tcaaccgttt gggaggacgg agccggcagg agaagtggat
     4021 tctccaatct gaacttttca acgagattca caaagtggtg cagtcgaacc gcaggttttt
     4081 cgaagaacgt tttcagctgc gcgccgaccg cgcttatggg ctgatccggg attatttaaa
     4141 agccgtgcga gaagtaatga attccatctg gggcgacaac gaaaggtata tgttcacccg
     4201 ggacgtgacg cttaaagcgc tcttgcgggt attcggcgat ttgatccgag accgcaagct
     4261 tgtccaagtt tgggaggaaa gccgggaccc ccgagcgttc gccggcgcca tgcggccgtg
     4321 ggcggaattg accagggagt ttcgcgccga aggtttttac gagcgtttcc ctgccaaagg
     4381 gcaggtggaa c
//
LOCUS       DASSWZ010000211         5758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_715154, whole genome shotgun sequence.
ACCESSION   DASSWZ010000211 DASSWZ010000000
VERSION     DASSWZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715154"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="metK"
                     /locus_tag="VFN58_05155"
     CDS             complement(<1..817)
                     /gene="metK"
                     /locus_tag="VFN58_05155"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET8917411.1"
                     /translation="MAGKDFLFTSESVSEGHPDKLCDQVSDAILDAHLAGDPDSRVAC
                     ESLAKTGMVVVAGEITSKAKVNYADVVREVVRDIGYTDSAMGFDGNTCAILTAVEQQS
                     PDISQGVNEGEGLHKEQGAGDQGMMFGYACDETPELMPFPIQMAHGLVQYLAKIRKGG
                     EAYFLRPDSKSQVTVEYRDGKPVKVQSVVISSQHSPDVEYNKLKETIIEGVIKAVIPA
                     EYLDKNTVYHVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDP"
     gene            complement(883..1158)
                     /locus_tag="VFN58_05160"
     CDS             complement(883..1158)
                     /locus_tag="VFN58_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET8917412.1"
                     /translation="MSRVIKKLEIKNKLGLHARAAALLVQTVNKFGAQVSFSKDGQTT
                     NGRSIMGVLTLAATQGSKIQVEASGEDAERVVRAIEKLIDRKFNENE"
     gene            complement(1163..2017)
                     /gene="rapZ"
                     /locus_tag="VFN58_05165"
     CDS             complement(1163..2017)
                     /gene="rapZ"
                     /locus_tag="VFN58_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET8917413.1"
                     /translation="MDIFIITGLSGSGKSVAIHALEDNGFFCIDNLPALLIPKFIDLC
                     QGYQEEIKRIALGVDLRGGQFLQSWPEVLTEMRAAGHHVQVLFFDASDEVLLRRFNET
                     RRPHPRAGQGSIQEGITRERKALEGMRELADKVIDTSDLNVHQLKREMEENFCQISDS
                     RRMTVFLTSFGYKYGIPHDTDIIFDVRFLPNPYFVQELRGKSGLESEVKEYVLKNEET
                     RAFLDRLDALLEFTLPLYEREGKSSLTVALGCTGGRHRSVVLVEELGKRLSQDKFPVH
                     AKHRDIDK"
     gene            complement(2036..2497)
                     /locus_tag="VFN58_05170"
     CDS             complement(2036..2497)
                     /locus_tag="VFN58_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET8917414.1"
                     /translation="MKITDFLTEEAVIPALTAREKNAVLKEMATALVASHPALDEKKV
                     LEVLLERERISTTAIGEGVAIPHGKLRGVERVVGIFARSLEGVDFASLDGGPTHLFFV
                     LIAPENAAADHLKALARISRLLKDPTFRSRLIEGKTGKEIFEIIAQEDQKF"
     gene            complement(2494..2811)
                     /gene="raiA"
                     /locus_tag="VFN58_05175"
     CDS             complement(2494..2811)
                     /gene="raiA"
                     /locus_tag="VFN58_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET8917415.1"
                     /translation="MSDVKVAVTFRHTQPTDALKHYAEQKIYRIGRHFSLSLEAHVVL
                     SVDSKERHTAEIELHTHGAMIHGKEETDDLYSAIDLVIDKLQRQVQKQKEKTKLNRRR
                     AKE"
     gene            complement(2835..4307)
                     /gene="rpoN"
                     /locus_tag="VFN58_05180"
     CDS             complement(2835..4307)
                     /gene="rpoN"
                     /locus_tag="VFN58_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET8917416.1"
                     /translation="MALEIRQVQKLAQQLVMTPQLQQAIKLLQLSRVELQEMISKELQ
                     ENPTLEEGTAEETENGDQPKSELPPDGAEPLTEPVINREPAVDKIGTLDWQEYLDTHS
                     NSIHGSLTAEATGEDGDSPPSWENSLTKKSSLEDHLIWQLRLSKISEREENIGFYIIG
                     NLDENGYLALSSEELCRATESSPEEIEQVLKRIQFFDPVGVAARDLRECLLVQLENLQ
                     FGDSLAARIVSDYLNYLESKRYEKMARELGVTVDEIADAAHVIASLEPKPSRGFGQEE
                     VLTVLPDVFVEKIGNEYVIYLNDDGVPRLRVSSLYRRMVGQEGAAEEQARQYLQEKVR
                     AATWLIKSIQQRQQTLYRVTQSIFKFQLEFLDHGVSHLRPMVLRDVAEDIHMHESTVS
                     RATANKYVHTPQGLFELKFFFQSGLKSGNGDDVASESVKEKIRNIIASEDPRKPHSDQ
                     HIAALLSNDSIDIARRTVAKYREAMGILPSSKRRQPYFRK"
     gene            complement(4310..5032)
                     /gene="lptB"
                     /locus_tag="VFN58_05185"
     CDS             complement(4310..5032)
                     /gene="lptB"
                     /locus_tag="VFN58_05185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET8917417.1"
                     /translation="MSALIAEAVAKSYSGRRVVNGVSIEVKGGQIVGLLGPNGAGKTT
                     TFHIMVGLVRPDEGKVLLNDQDLTGAPIYQRARAGISYLPQESSVFRRLTVEENLFAI
                     LQTLDLSGAQQQERCQALMKMLGVDHLADHKAFTLSGGERRRVEIARALVLSPYFVLL
                     DEPFTGVDPIAVAEIQKIIRRLIVSGIGILITDHNVRETLGICDRAYILSDGTVLEEG
                     TPEKIASSPKARKFYLGEGFKF"
     gene            complement(5035..5604)
                     /gene="lptA"
                     /locus_tag="VFN58_05190"
     CDS             complement(5035..5604)
                     /gene="lptA"
                     /locus_tag="VFN58_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HET8917418.1"
                     /translation="MGYSMLGLSLFFSLLSASAVMTPGDAATPVKDESKTKGGTAFEF
                     NRKDPIYINSDWMEFDQTKNTITYKGRVVTTQGEMTLRSETLTAEYTENMKQIKQMVA
                     EGKVNVTQGNRVATGEKVVFDDQAKTVTLTGSPIMRQGNNQISGTKVIYFVEQDRAEV
                     EGDGKVRVQATIFPEELGKQDKGDSASSN"
BASE COUNT         1257 a         1648 c         1356 g         1497 t
ORIGIN      
        1 aaggatcctt gcccgaaaaa gcgcctccgc cgtggcgtcc catacctcca taggtgtcga
       61 cgataatctt gcgtccggtg agaccgcaat cgccttgagg accgccgacg acaaagcggc
      121 ccgttgggtt tacgtggtag acggtattct tatcgagata ttccgccgga atcaccgctt
      181 tgattacgcc ttcgatgatc gtttccttga gtttattgta ttcgacgtca ggactgtgct
      241 ggctggaaat aaccacgctc tgaaccttta ccggtttacc gtcgcgatat tcaacggtga
      301 cctgagactt gctgtcaggc ctgaggaagt aagcttcgcc gcccttgcga atcttagcca
      361 ggtactggac caatccgtga gccatctgga tcggaaatgg catcagttcc ggggtctcat
      421 cgcaggcgta accgaacatc attccctgat cgcccgcccc ttgttctttg tgcagtccct
      481 cgccttcgtt taccccctga gagatatccg gcgattgctg ctcgacggca gtcagaatcg
      541 cgcaggtgtt gccgtcaaac cccatggcgg aatcggtata gccgatatcc cggacgactt
      601 cgcgaacgac gtcggcgtaa ttcactttgg ctttgctggt gatttcgccc gcgacaacca
      661 ccatcccggt cttcgctaga ctttcgcagg cgacccggct gtccggatcc cccgccaagt
      721 gagcgtctag aatcgcatcc gagacttggt cgcagagctt gtccggatga ccctcagaga
      781 ccgactctga agtaaataaa aaatccttgc ctgccattta agttacctcc agggtctaaa
      841 tgaatcagct ttctatatcg gataagaggg gtcgattcaa cctcactcgt tttcgttgaa
      901 ctttctgtca atcaattttt cgatggcccg caccacccgt tccgcatcct caccgctggc
      961 ctcgacttgg atcttacttc cctgcgtcgc agccaacgtc agcacgccca taatgctccg
     1021 cccgttggtt gtctgaccgt ctttggaaaa actgacttgg gctccgaact tatttacggt
     1081 ttgcaccagt aaggccgccg ctcgagcatg gagcccgagc ttgttcttga tctccaactt
     1141 cttaataact ctactcattg agctatttgt cgatatctcg gtgcttagcg tggacgggga
     1201 atttatcctg gcttaacctt ttccctagct cttcaaccaa cacgacggag cgatgccgtc
     1261 ccccggtaca ccccaaggcg acggttaaac tgctttttcc ctcccgctcg tagagcggca
     1321 aagtaaactc caaaagcgcg tcaaggcgat caagaaatgc cctggtctcc tcgtttttaa
     1381 gaacatactc cttaacctcg gactctagtc cgctttttcc tcggagttcc tggacaaaat
     1441 acggattcgg cagaaatcga acatcgaaaa tgatatcggt gtcgtgggga atgccatatt
     1501 tgtacccgaa ggaggtcagg aacacagtca tgcggcggga gtctgagatc tggcaaaaat
     1561 tctcctccat ctctctcttt agctggtgaa cattcaaatc gctcgtatcg atcaccttat
     1621 ccgcgagttc ccgcatccct tccaatgctt tacgctcccg cgtaatgcct tcctgaatcg
     1681 atccttgacc cgcgcgcgga tgcggccgcc gcgtctcatt aaatcggcgc agcaacacct
     1741 catccgacgc atcaaaaaaa agcacctgga cgtgatggcc cgcggcgcgc atctcagtta
     1801 agacttcggg ccaagattgc aggaactgtc cgcctcttaa atcgacgccg agggcgatcc
     1861 ttttgatctc ctcttgatac ccctggcaaa ggtcaatgaa ttttggaatt aacagcgccg
     1921 gcagattgtc gatgcaaaag aacccgttgt cttcaagagc atgaatcgca acgctctttc
     1981 ccgaaccgga aagcccggta atgatgaaga tgtccagacc atcgaccata agcggtcaga
     2041 acttttgatc ttcttgggcg atgatttcga agatttcttt tcccgttttc ccttcgataa
     2101 gccggctgcg aaaagtggga tcctttagca atcgggaaat tctcgccaag gccttcaggt
     2161 gatcggcggc ggcattctcc ggggcgatca gcacaaaaaa caagtgcgtc ggtccgccat
     2221 cgagagaagc gaaatcgact ccctccagac tacgggcgaa gattccgacc acgcgttcga
     2281 cccccctaag ctttccatgc gggatcgcca caccttcacc gatggccgtt gtgctgatcc
     2341 gctcgcgttc caacaagacc tcgagaacct ttttctcgtc aagcgctgga tggctagcga
     2401 ccaacgcggt tgccatttct tttaggacag cgtttttttc gcgcgccgtg agggcaggaa
     2461 taaccgcttc ctcggtcaga aaatctgtaa tcttcactct ttcgcccttc tccgattaag
     2521 cttggtcttt tccttttgct tttgcacctg gcgctgaagc ttgtcaatca ctaagtcaat
     2581 ggctgaatag agatcgtcgg tttcttcctt gccatggatc atggctccgt gcgtatgcaa
     2641 ctcgatctcc gccgtatggc gttccttaga gtccacggaa agcaccacat gggcctccaa
     2701 cgacagtgag aaatgccttc cgatgcggta gattttttgc tcagcatagt gtttcaacgc
     2761 atcggtaggt tgcgtatggc gaaaagtcac cgcaactttc acgtcgctca ttgtgaaatt
     2821 tacccctcca gcacttattt tctgaaatag ggctgtctcc gcttcgacga gggaagaatg
     2881 cccatggcct cccgatactt ggccaccgta cggcgtgcga tatcgataga atcgttcgac
     2941 aagagcgcgg caatgtgttg gtcgctgtga ggctttcgcg ggtcttcgga ggcgataatg
     3001 ttacggattt tttccttcac actctcgctc gcgacgtcgt ccccgttgcc gcttttgaga
     3061 ccgctttgga aaaagaactt gagttcaaaa agcccctgcg gtgtgtgcac gtacttgttt
     3121 gccgtggctc gactcaccgt ggattcatgc atgtggatgt cttcagcgac atctcgaagc
     3181 accatgggtc ttaggtgact gactccgtga tcgagaaatt ccagctgaaa tttgaaaatg
     3241 ctttgagtaa cacggtagag ggtttgctgt cgctgctgaa tgctcttgat caaccatgta
     3301 gctgcgcgca ccttctcttg aaggtattgg cgtgcctgtt cttccgccgc cccttcctgg
     3361 ccaaccatgc gccggtaaag tgaacttacc cttaaccgcg gcacaccgtc gtcgttgagg
     3421 tagatgacgt attcgttgcc gattttttcc acgaagacgt cgggtaaaac ggtaagaact
     3481 tcctcctgcc cgaaaccacg cgatggcttc ggctcaaggg atgcaatcac atgggcggcg
     3541 tccgcgattt catccacggt gacacccagc tcgcgagcca tcttttcgta ccgtttggat
     3601 tccaggtaat tcagataatc gctcacgatt cgagccgcca aactgtcacc gaactggagg
     3661 ttctcaagct ggaccaagag acattcccgc agatcacgcg ccgcaacccc taccggatca
     3721 aagaattgaa tgcgctttag aacttgctct atttcctcag ggctactttc cgtagccctg
     3781 cagagttcct ccgaggaaag ggccaggtaa ccattctcgt ccaagttgcc gatgatataa
     3841 aaaccgatat tctcttcacg ttcggagatc ttcgagagac ggagttgcca aatcagatga
     3901 tcttcgagcg agctcttctt ggttaagcta ttctcccagg atggcgggct gtcaccatcc
     3961 tccccggtgg cttcggcggt cagagatccg tggatggagt tcgaatgggt atcgagatat
     4021 tcttgccagt ccaacgtacc gatcttgtca acggcaggtt cgcggttgat cacaggttcc
     4081 gttagtggct ccgcgccgtc tggcggcaac tccgacttgg gttgatcccc gttctcggtc
     4141 tcttcagcgg ttccttcttc caaggtggga ttttcctgca actccttcga aatcatctcc
     4201 tgtagttcga cacgggacaa ctgaagcagc ttgatcgcct gctgcagctg aggtgtcatg
     4261 accaattgct gagcgagttt ctggacctga cgaatctcta gagccataac tagaacttaa
     4321 aaccttctcc caaataaaat ttcctcgcct tggggctcga ggcgatcttc tccggcgttc
     4381 cttcctctaa aacagtgccg tcgctaagaa tataagctcg gtcgcaaatt cccaatgtct
     4441 cccgaacatt gtggtcggtg atgagaattc cgatcccgct cacgatcaat ctgcggatga
     4501 ttttctgaat ttctgcaact gcaatgggat ccacaccggt aaaaggttcg tccaggagaa
     4561 caaaataagg agaaagaacc aacgcacggg cgatttcgac tctccgtcgc tctccacccg
     4621 acaatgtaaa tgccttgtga tcggcgaggt gatctacacc gagcatcttc atgagcgcct
     4681 gacatctctc ttgctgttgc gcgccgctca gatcaagggt ctgcaaaatc gcaaagagat
     4741 tttcttctac ggttaggcga cgaaaaaccg aggattcctg cggaagatag cttattcccg
     4801 ctcgtgcccg ctggtaaatg ggcgcaccgg tgagatcctg gtcgttcaaa agcaccttac
     4861 cctcgtcagg tcgaaccaag ccgaccatga tatgaaacgt cgtggttttc cctgcgccat
     4921 ttggaccaag caaaccgaca atttgaccgc ctttaacctc aatactaact ccattgacga
     4981 cgcgacgacc actgtaggat tttgccaccg cctcagctat aagagcgctc atctctaatt
     5041 gctcgaagcg gagtctcctt tgtcctgctt gcctagctct tcaggaaata tcgtcgcctg
     5101 tactctcact ttgccgtctc cttcaacctc cgctcgatcc tgttcgacaa aatagatgac
     5161 tttcgttcca ctgatttggt tattgccttg ccgcataatg ggactacccg tcaaagtcac
     5221 cgtcttggcc tggtcgtcaa aaaccacttt ctcccccgtg gccacccgat tgccttgcgt
     5281 gacatttacc ttcccctcgg caaccatttg cttgatctgt ttcatattct cagtatattc
     5341 ggccgttagg gtctcacttc ttaacgtcat ctctccttgg gtcgtaacca cacgcccctt
     5401 ataagtgatc gtgttcttgg tttgatcaaa ctccatccaa tcggagttga tataaatggg
     5461 atcctttcta ttgaattcaa acgccgtacc gccctttgtt ttcgactcat ctttcacggg
     5521 cgtcgcagca tcgcctggcg tcatgaccgc agaagcggat aagagggaaa aaaaaagcga
     5581 taaccctagc attgaataac ccatctaccc tccgctcatc tggttagggt tagatccttt
     5641 gtctttcctc gacaccttat cgggctcgat cttggtcttg acgttctgca ccaagcgcac
     5701 ttttttgact tccagatcaa cctccatcct ggatccttct atttccaggc cgttcccc
//
LOCUS       DASSWZ010000212         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_262404, whole genome shotgun sequence.
ACCESSION   DASSWZ010000212 DASSWZ010000000
VERSION     DASSWZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262404"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFN58_05195"
     CDS             complement(<1..222)
                     /locus_tag="VFN58_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET8917419.1"
                     /translation="MEIKSLIDPEGGLVNRRIFADREIYELERERLFARCWLYLGHES
                     EVPHPGDFVTAYMGEEPIILCRDLSGRLRA"
     gene            complement(305..1345)
                     /locus_tag="VFN58_05200"
     CDS             complement(305..1345)
                     /locus_tag="VFN58_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917420.1"
                     /translation="MLLPFREHLDFGPTPYDQLQAKEGIPVIRGNVVSDVMELELGRW
                     ERMGVAGCYLNLAEQQQTDAYVCEIPPGNETLPQRHLFEEIVYIVKGRGATSVWQEGA
                     SKVTFEWGPGAVFAIPLNSSYQHFNGSRDEPVRLFAGTTCPRMINIFHNEDFIFRNPF
                     NFRDRFHDGQEFLRFNKHMADRYWETNLVPDVNQFALDDFPMKGKGVKHMRYTLADTT
                     YGCHVSEFPPGSRSTFHRHGPGAVIIITQGEGYVMLWKDGEPRVRHEFHVGTVYSPGD
                     LMWHGHFNTGKGTMRHFAMRGDSPKYSHDRFRNPLWTMIPMDEEPPEIHRDYVQELAR
                     KGVRAAVSVVED"
     gene            complement(1439..>2386)
                     /locus_tag="VFN58_05205"
     CDS             complement(1439..>2386)
                     /locus_tag="VFN58_05205"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET8917421.1"
                     /translation="AKFSAGEIRVCHVPKTIDNDLPLPGETPTFGFETARQHGFEIVH
                     NLMEDSRTTHRWYFVVVMGRTAGHLALGVGKAAGATLSVIPEEFPGAVHLETVGDLLE
                     GAILKRQALWQRYDGVAVIAEGLLERMPVEELTRIEGVQIQHDSYGHLRLAELDLAYI
                     LKNLVERRFAERGRNLTIVHKTIGYELRCAPPVAFDCEYVKTLGYGAVEFLLNPNEST
                     SGVTGALVCIVDGQLRYLKFEDLMDSKTNKTSVRPVDISKPAYKVAREYMIRLEREDF
                     ENPERLAKLARTASSREKPLATEAFKRKFEHVLTDLRWP"
BASE COUNT          528 a          663 c          640 g          555 t
ORIGIN      
        1 ggcgcggaga cgcccgctca gatcgcggca caagatgatc ggctcttccc ccatatacgc
       61 ggtaacgaag tcgccgggat ggggcacctc gctttcgtgg ccaaggtaga gccaacagcg
      121 ggcgaaaaga cgctcgcgtt ccagctcgta gatctcccgg tcagcaaaaa tgcgccggtt
      181 taccagaccg ccttctggat cgataagaga tttgatctcc atccggatac tccttcggtt
      241 ctcacgttgc cgcgaagaga attgcaggat tgcgttctga atccattatt ccatcattcc
      301 agtcctagtc ctctaccacc gagaccgcgg ctctaactcc ttttctcgcc agctcttgca
      361 cgtagtcgcg atgaatctcg ggaggctcct cgtccatggg gatcatcgtc cagagcggat
      421 tgcgaaaccg gtcgtggctg tatttcggac tgtcaccccg catggcaaag tggcgcatgg
      481 ttcccttgcc ggtattgaaa tgaccgtgcc acatgaggtc tccaggtgaa tagacggtgc
      541 cgacgtggaa ctcgtggcgc acccgaggct caccgtcctt ccagagcatc acgtagccct
      601 caccctgggt gatgatgatc acggcgccgg gaccgtggcg gtgaaaagtg ctgcgggacc
      661 ccggcggaaa ttccgaaaca tgacaaccgt aggtggtgtc cgccagggta tagcgcatgt
      721 gctttacccc tttgcccttc atcggaaaat catcgagagc aaattgattc acgtcaggaa
      781 cgagattggt ttcccaatat cggtccgcca tatgcttatt aaatcgcaaa aactcctggc
      841 cgtcgtgaaa acggtcgcga aagttaaacg gattacgaaa gataaaatcc tcattgtgaa
      901 aaatattgat catgcgtggg caggtggttc cggcgaacaa acggaccggt tcgtcccttg
      961 agccgttgaa atgctggtag gaggagttca gggggatggc gaagaccgcg cccggtcccc
     1021 attcgaaagt gactttggag gctccctctt gccataccga ggtggcgccc cgccctttga
     1081 cgatatagac gatctcttcg aaaagatggc gttgcggcag agtttcgttg cccggcggga
     1141 tctcacagac ataggcgtcg gtttgttgtt gttcggcaag attgagatag caacccgcaa
     1201 cgcccatccg ctcccatcgt cccagctcca attccataac gtcgctgacg acgtttcccc
     1261 ggatgaccgg aattccttcc ttggcttgga gctgatcgta aggggtcggt ccaaagtcga
     1321 gatgttcccg gaaaggcaag agcatgtttg aatctcctct acggtctcca tttctaaaca
     1381 caatcatcct ttggagacaa ccgggtcgat caatgtagcg cgttattctg ctgttattct
     1441 acggccagcg aagatcggtc agaacatgct cgaattttct tttgaacgct tccgtggcca
     1501 gcggtttttc tcgacttgaa gcagtgcgcg ccaatttggc gagacgttcc ggattttcaa
     1561 agtcctctcg ttccaatcga atcatgtatt cacgggccac tttgtaggcc ggtttggaga
     1621 tatcgacggg ccgcaccgag gtcttgttgg tcttggaatc catgaggtcc tcgaacttga
     1681 gataacgcaa ctggccatcg acaatgcaga ccagggctcc ggtgactcct gacgtcgatt
     1741 cgttggggtt taaaagaaat tctactgcgc catagcctaa ggtcttcaca tactcgcaat
     1801 caaacgccac cggtggagcg caacgcaact catagccgat ggttttgtgc acgatagtaa
     1861 gattccgccc gcgctccgca aagcgtcgct caaccagatt tttcaaaatg taggcgagat
     1921 caagctccgc caggcgtaga tgaccgtagc tatcgtgctg aatttgcacg ccctcgatcc
     1981 ttgtaagttc ttccaccggc atgcgctcaa gtaggccttc agcgatcacc gcgacgccat
     2041 cataccgttg ccataaggct tgccgcttta ggattgcgcc ttccaaaaga tctcctacag
     2101 tttcgaggtg aaccgcgccg gggaattctt ccggtatcac ggaaagtgtc gcgccggcgg
     2161 ccttgccgac gccgagcgct agatggcctg cggttcgtcc catgaccaca acaaagtacc
     2221 agcgatgagt ggtgcgggaa tcctccatca agttatggac gatttcgaaa ccgtgctgtc
     2281 gcgcggtttc aaagccgaat gtcggcgtct ctcccggaag gggcagatca ttatcgatgg
     2341 ttttaggtac gtgacacact cgaatctctc cagccgagaa cttggc
//
LOCUS       DASSWZ010000213         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1097188, whole genome shotgun sequence.
ACCESSION   DASSWZ010000213 DASSWZ010000000
VERSION     DASSWZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097188"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="VFN58_05210"
     CDS             complement(<1..1405)
                     /locus_tag="VFN58_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8917422.1"
                     /translation="MTNKKKYSSLELYRRLFLEIRPYRLHIGAILLVTLLSPPLSLLI
                     PLPLKIAVDSVIGSHPIPAFLQPVIPQAIIGSPGNMLTFVIVLMMVVALLGGLQRVAS
                     TWLRTYTGEKLTLAFRNKLFRHVQRLSVSYHDTRGTTDSTYRIQYDAPAIQWIAVDGF
                     IPFVSSVLTLAGMIYIITLLDWQLAVVAIAISPILYLGARTFNSRLRNQWVDIMHLES
                     SAFSVVQEALGALRVVKAFGQEEREQERFVRHSSESFLVRVRAAWSEGGFDLLIGVTT
                     AAGTAAVLFLGVRHVQSGTLTLGELMMVMSYLAQLFGPLASLSEMTARAQRSLASAER
                     AFALLDEAHDVVENKNGVPISRASGALTLQNVCFAYNGEYPVLSDISFAIKPGTRVGI
                     MGMTGGGKSTLVSLLTRFHDPTKGQILLDGVDLRNYRLTDLRNQFGIVLQESILFSTS
                     IGENIAYARPGALQHEIV"
     gene            complement(1380..>3279)
                     /locus_tag="VFN58_05215"
     CDS             complement(1380..>3279)
                     /locus_tag="VFN58_05215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8917423.1"
                     /translation="YLARLHNIAGDRPLLMSEIGLDSLRNGEATQAQALEWQLRSTFS
                     PGCAGAFIFSWTDEWHRAGAEVEDWAFGITARDRRPKPALKTVREAFTNIPFAKELRW
                     PKISVIVCTFNGSRTLSECLESLLRLEYPNYEVIVVNDGSTDTTAKIANHYGFRVITT
                     ENRGLASARNTGLAAATGEIVAYIDDDAYPDPHWLRYLAGTFMNTKHVGIGGPNIAPP
                     SDGSIAECVAHSPGNPVHILLSDTEAEHIPGCNMAFRKANLEAIDGFDPQFRIAGDDV
                     DVCWRLQEKGWTLGYSPGAMVWHHRRNSIRAYWKQQHNYGKAESFLEKKWPEKYNLAG
                     HVTWAGRVYGNGHQYKAWSQGRIYHGVWGSAPFQSIYQSPAGALESWLSMPEWYLALA
                     ALAPISALGFLWSPLFYALPLLALALGAPIFQAVVSASRVCFAVPLRSYFGRFKLRNT
                     TALLHLIQPLARLTGRLRSGLTFWRYSASGFTMPWPRKFALWTEHWRDPNERLKSFEA
                     DLRKAGVHVRRGGDYDRWDLEVRAGLLGFARLLMAVEDHGAGNQFVRVRLWPQCSLLE
                     LFPIVLFASLSAAAALDHAWVASAIMGLLSLFLTLYSLRGCGSAVAAILRTLQHSDGI
                     HDEQKEIQ"
BASE COUNT          835 a          881 c          868 g          695 t
ORIGIN      
        1 caacgatttc atgttgcaag gcaccgggac gtgcataggc aatgttctct ccgatactgg
       61 tcgaaaaaag tatagattcc tgaaggacga tcccgaactg gttacgaaga tcggttagtc
      121 tatagtttcg cagatcgaca ccatcgagta ggatttggcc tttagtcggg tcgtggaatc
      181 tagtcaacaa actaacaagc gttgacttcc ccccacctgt catacccata atacctactc
      241 gagtgccggg tttaattgcg aacgagatgt cactcaacac cggatattcg ccgttatatg
      301 caaagcaaac gttctgcagg gttagcgctc cgctagcacg agatatcggc actccatttt
      361 tattttcaac gacatcgtgg gcttcatcca aaagggcaaa tgcgcgctcc gcgctggcaa
      421 gcgagcgttg cgcacgcgcc gtcatctcgc taagagaagc caggggtccg aatagctgtg
      481 ccaagtagga catcaccatc atcaactcgc ccaatgttaa tgtgcctgac tggacatgcc
      541 gcacgcccag aaataggacc gcagctgttc cggctgccgt ggtaactcca atcagaaggt
      601 cgaaacctcc ttccgaccat gcagcgcgaa cccgcacaag aaagctttcg ctggagtgac
      661 gaacaaaacg ctcttgttct cgctcttctt ggccgaaggc tttcaccacg cgcagcgcgc
      721 ccagagcttc ctgcactact gaaaaagcgg aactttccaa atgcataata tctacccact
      781 ggttgcgaag ccgcgaatta aacgtccgcg caccaaggta caaaataggt gaaattgcaa
      841 ttgcgacgac tgccaactgc cagtcgagca gggtgatgat gtaaatcatc ccagcgaggg
      901 ttagtactga agagacgaaa ggtatgaaac catcaacggc tatccactga atcgcaggag
      961 catcatactg gatccgatac gtggaatcag tcgttcctct cgtatcgtgg tacgaaaccg
     1021 aaagacgctg gacgtggcga aagagtttgt ttcggaatgc caaggttaac ttttctcccg
     1081 tataggtgcg caaccacgta cttgcgaccc gttggagtcc gccgagaagg gcaacgacca
     1141 tcattagaac gatgacgaat gtcagcatgt taccaggcga gcctatgata gcctgtggta
     1201 tcactggctg tagaaacgct ggtattggat gggagccgat gacgctatcg acggcgattt
     1261 tcagtggcaa tgggatcagc agagagagcg ggggcgataa caaagttacc agaagaatcg
     1321 cccctatgtg caagcgatac ggccttattt caaggaacag ccgacggtaa agttcaagac
     1381 tactgtattt ctttttgttc gtcatgaatg ccatccgaat gctggagggt gcgaaggatt
     1441 gcggcaacgg cgctcccgca gccgcgcaga ctgtatagcg tcagaaaaag gctcaacaaa
     1501 cccatgatgg cggaagccac ccatgcgtga tcaagagctg ccgccgcgga aagggaagcg
     1561 aataaaacaa taggaaaaag ctctaacaag gaacactgag gccagagccg aacgcggaca
     1621 aattggttac cggcaccgtg atcctccacc gccatgagca agcgcgcgaa gccaagcaac
     1681 cccgcacgca cttccaagtc ccatcgatcg taatctccgc cgcgccgaac atgaacgccc
     1741 gccttgcgta aatccgcttc gaaagatttc aatctttcat tgggatcgcg ccaatgctcg
     1801 gtccaaaggg caaatttccg cggccaaggc atcgtaaagc cggatgcgct gtatctccag
     1861 aaagtaaggc cggagcgcag gcgtccggta aggcgcgcca acggttgaat taaatgaagc
     1921 agcgctgtcg tgttgcgtaa cttgaaccgg ccaaagtagg acctgagagg gactgcaaag
     1981 cagacgcgtg aagcgcttac cacagcttgg aaaatcggcg cgcctagtgc tagcgctagt
     2041 aaaggcaagg cgtagaacaa cggagaccaa aggaatccga gtgccgaaat cggagcaagc
     2101 gccgcgaggg ccaagtacca ttccggcatg gacagccaag attctaaggc cccggcaggc
     2161 gactgataga tagattgaaa gggcgcgctg ccccatacgc catgataaat tcgtccttgg
     2221 ctccatgcct tatattggtg gccattcccg taaacgcggc ccgcccatgt gacatggccg
     2281 gcgagattgt acttctccgg ccattttttc tccaagaagg attccgcttt gccgtaattg
     2341 tgctgttgct tccagtaggc tcgaatcgaa ttgcgtcggt ggtgccagac catcgcccca
     2401 gggctatatc ctagcgtcca tcctttttct tgaagtcgcc agcagacatc cacatcatcc
     2461 cccgctatgc gaaattgcgg atcaaatccg tctatggctt ccagattggc tttgcgaaaa
     2521 gccatgttgc accccggaat atgttcggct tcagtgtcgg acagcagaat gtggaccgga
     2581 tttcccggag agtgggcaac gcactcggca atagacccat cgctgggggg cgcgatgttc
     2641 ggtccgccga ttccaacatg tttcgtgttc atgaacgtac cggcaaggta tcgtaaccaa
     2701 tgtgggtcgg ggtatgcgtc atcgtcgata tacgccacga tttctccggt ggctgccgcc
     2761 agtcccgtat tgcgtgccga agcgaggccc cgattttcgg ttgttatcac tcgaaagcca
     2821 taatgattcg cgatttttgc agtagtatcc gttgagccat cgttcaccac gatcacctcg
     2881 tagttgggat actcaagccg taataaactc tccaggcatt cagaaagagt tcggctgcca
     2941 ttaaaggtgc atacaataac ggatatcttc ggccagcgga gttccttagc gaaagggatg
     3001 ttggtgaacg cctctcgaac agtcttgaga gcaggtttgg gacgacgatc ccgggcagtg
     3061 ataccaaatg cccaatcttc gacttcggca ccggccctgt gccactcatc agtccacgaa
     3121 aatataaagg cccctgcaca accgggtgag aaggtgctcc ggagttgcca ctcgagcgct
     3181 tgcgcctgag ttgcctctcc gtttcgaaga ctatcgaggc cgatctcgct catgagtaga
     3241 ggtcgatcac cggcaatatt atgaagccgg gccaagtac
//
LOCUS       DASSWZ010000214         7775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_800278, whole genome shotgun sequence.
ACCESSION   DASSWZ010000214 DASSWZ010000000
VERSION     DASSWZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800278"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFN58_05220"
     CDS             <1..456
                     /locus_tag="VFN58_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET8917424.1"
                     /translation="TLEIGKAELLRDGSDVVIAAIGQTVIPALKAAQDLAALGMDAAV
                     VNARFLKPLDKDLLRRLLASISNLITVEDHSVAGGFGSAILEFLADEGISHVRVKRLG
                     VPDRFIPHGTQDELRKICGFDKDAITQATLQMLRRGKKRNREGWERGSA"
     gene            435..1178
                     /locus_tag="VFN58_05225"
     CDS             435..1178
                     /locus_tag="VFN58_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET8917425.1"
                     /translation="MGKRERLDKILVERRLVASREEGRGRILAGEVWVNDRPATKAGT
                     LFDESVVIRVKSASPYVSRGGAKLEKALDEFSVDLTGKIVLDVGASTGGFTHCLLTHG
                     AVQVYAVDVGYGQLDWKLRNDPRVLVLEKRNIRYLNVSDLPRPADLATIDVSFISLRL
                     VLPQVKTLLHAGSPVIALIKPQFEVGKGKVGKGGVVRSHPEHMRVVDEISTAARGLGY
                     HVCGVIESPLLGPKGNKEFFIHLKARELR"
     gene            1228..1962
                     /locus_tag="VFN58_05230"
     CDS             1228..1962
                     /locus_tag="VFN58_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET8917426.1"
                     /translation="MVAFVKVAHRGASGNFPENTRLAFEKAIEAGVDMIEMDCQLTRD
                     GHVVVFHDDRLKRTAGVSGTVRSKSLEQLKQLDVGQWKKKSFRGERVLTLEEVLAIIG
                     GKVDLCLDIKQFKDSQPGIEIKLLFTLSHYDYLDQTIFSSFNYQCLDRVREFAPEARI
                     GIIYGTGIKEDPFGAAERLGAGSIHVQKEAATRPFLDKAWEAGLDIHVWTVNNVHDME
                     KFAALGVQGIVSDYPEKLCNPKLLHR"
     gene            2259..2453
                     /gene="rpsU"
                     /locus_tag="VFN58_05235"
     CDS             2259..2453
                     /gene="rpsU"
                     /locus_tag="VFN58_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET8917427.1"
                     /translation="MTGVRVRENESIESAIRRFKKLCEKAGILAELRKREHYEKPSVK
                     RKKKAIAAKKRALRRVRHSF"
     gene            2502..2948
                     /locus_tag="VFN58_05240"
     CDS             2502..2948
                     /locus_tag="VFN58_05240"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET8917428.1"
                     /translation="MGLKVEIQESMKAAMKTGDRLTLSALRLVLSALHNEEIKVRREL
                     TTEEIHKTITTLCKQRTEAIELFRKGGRAELAEKEEAELKVLQRFLPQPLSEDEVRNL
                     IKVSIEEVGAKNIQDLGKVMKQLMPRVSGRTDGKRVNELARELLGG"
     gene            3202..3735
                     /locus_tag="VFN58_05245"
     CDS             3202..3735
                     /locus_tag="VFN58_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET8917429.1"
                     /translation="MNWIDLILLVVLFLFGLRGYFKGLFRETLSLAGLVIGFMVAVRY
                     DEAVAAWVGFYWNVSSIILKGAAFVVIFFVVYFIFAVAGWSLHRAAKVLFLQTINRLG
                     GIAVGLGKGTAVTALIIFFVTSSAWIPRSTRERVEDSYLGPPLSQLAVKLIRVGKEKL
                     LPEESGRALIQLRVCCS"
     gene            3745..5547
                     /gene="dnaG"
                     /locus_tag="VFN58_05250"
     CDS             3745..5547
                     /gene="dnaG"
                     /locus_tag="VFN58_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET8917430.1"
                     /translation="MIKQEKITEIRDRASIVEVISDYVTLKKAGRNYQGLCPFHGEKT
                     PSFTVSEEKGIFHCFGCQAGGSVFHFLMKYDQLSFPEAVERVAKRYGIKIESSDGVRE
                     PGDSGERESLYRINERAAANYHQILLSQPAGRKALDYLKSRKVNEEIAQRFMVGYAPQ
                     TGSGLLDLLRREKLSVKDAMRLGLIGQQSGQQFHEKFFARLMFPIINAGGKIVGFGGR
                     VLDQGLPKYINSIETPLFRKGSTLYGLYQAKEGIRRVDRVVVVEGYLDVMALHQFGLG
                     YAVATLGTALTPDHVRVLGRYTRNIVALFDGDDAGRKAAARSFEIFIETGLLGRAAFL
                     PKGEDPDTYIHGHGPEALEKILDEAVPLADYHFSSLTQRFGMSLEGKSQIAGEVSRLL
                     TKVNNPIDVDLLVRRAVDTLGVREEVLRRPIVTPGYSLRSKMIPKALSATPSPIRDDI
                     AERSLVSLMLRFPAVLRAVEKEGEARQWLSSKWQAVFDLILAEWQERRQVDVFRVAQR
                     VHPDQAPEITALALQGESILETESDKMAADCLSHLRRKYLRTLERNLRVAIRAAEERK
                     DEQAKRERILEWQDVVQKERQLERQRLEPKTTIR"
     gene            5475..7346
                     /gene="rpoD"
                     /locus_tag="VFN58_05255"
     CDS             5475..7346
                     /gene="rpoD"
                     /locus_tag="VFN58_05255"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET8917431.1"
                     /translation="MARRRTKRAPARTPKARAENNHPIATEKLASEHAGQPVERKNMK
                     EVKKLIDLGKEKGYLTYDDVNDMLPAEVVSPDQIDDVMSIFGEMDIEVVDANQRVSLG
                     GAGEDLVEDEEEEKEVESDIDGDMVGKTGDPVRMYLREMGTVSLLSREGEVEIAKKIE
                     QGEKQVIEEVLSSPLALRYVLDLGEKLAQREIRVREIIKDGDEDAEYLEDEEVHEKRL
                     LEQMAKIRRTANETEKIKRQLDGRRVSTPRRQSLLQNLAKYQEKIIGGLKALQLNRRQ
                     TEAIVDGLKRAMDRMQTLERRVKDFEQKTGKAAPDIIKLVSGVNGRRKDWQRLIGSLR
                     MGHDAIVTMAEEIKNARRDIRHIERDLEMPAEEIKRRVWSIIEGETRANLAKKELIEA
                     NLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTYATWWIRQAIT
                     RAIADQARTIRIPVHMIETINKLIRTSRYLVQEIGREPTPEEIASKMEIPLDKVRKVL
                     KIAKEPISLETPVGEEEDSHLGDFIEDKRIITPSDAVVNINLQEQTRKVLATLTPREE
                     QVLRMRFGIGEKSDHTLEEVGQKFTVTRERIRQIEAKALRKLRHPSRSKRLKSFMES"
     gene            7422..7498
                     /locus_tag="VFN58_05260"
     tRNA            7422..7498
                     /locus_tag="VFN58_05260"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7456..7458,aa:Ile,seq:cat)
BASE COUNT         1950 a         1855 c         2227 g         1743 t
ORIGIN      
        1 acattggaaa tcggtaaagc ggagctgcta cgcgatggca gtgatgtggt gatcgccgct
       61 atcggccaaa ccgtcattcc tgccctcaag gccgcccaag acttggcggc gttaggcatg
      121 gatgcagcgg ttgtgaacgc acgtttcctg aagcctctcg acaaagatct cttgcgccga
      181 ttgttagcaa gtatatctaa cctgattacc gtggaggatc attctgtagc tggcggtttc
      241 ggcagtgcta ttttggaatt cttggctgat gagggaatca gccatgttcg cgtgaagcga
      301 ctcggcgttc cggatcgctt catcccacac ggcacgcaag atgaactgag aaagatctgc
      361 ggcttcgaca aggatgccat cactcaagcg acattgcaga tgcttcggcg cggaaagaag
      421 agaaaccggg agggatggga aagagggagc gcctagacaa gattctggtc gagcgccgat
      481 tggtggccag ccgtgaagaa gggcggggga gaatcttagc tggcgaagtt tgggtgaacg
      541 atcggcccgc cacaaaagca gggacgctct ttgatgagag tgttgttatc cgggtcaaat
      601 cagcttcgcc ctacgtcagc cgcggcgggg ccaaattgga aaaggctctg gatgaattct
      661 cagtcgacct cactggcaaa atcgtattgg acgtcggcgc atccacgggg gggttcaccc
      721 attgtcttct gacccatggc gcagtgcagg tctatgccgt ggacgttggt tacggccagc
      781 tcgattggaa actgaggaac gatccgcgtg tgctagtctt agaaaagaga aacatccgct
      841 atctgaacgt ctcagattta ccccgtcccg cagatcttgc aactatcgac gtttccttca
      901 tttcccttcg gttggttctc ccgcaggtta agactcttct ccacgcaggt tccccggtga
      961 tcgccctaat caaacctcag ttcgaagtgg gaaaagggaa agtcgggaag gggggagtcg
     1021 tccgctcgca cccggagcat atgcgcgtgg ttgacgagat ttcgactgcc gcccgggggt
     1081 tgggctatca cgtctgcggt gtcatcgagt cgcctttgct tggcccgaag ggtaataagg
     1141 agttcttcat acatctcaag gcacgagaac tccggtgaaa agtttcaggt cttttgagaa
     1201 gttcgtattg ccaacccgcg agggcttatg gttgcctttg tcaaagtagc ccatcgcggt
     1261 gcctcaggca attttccaga aaatacccgg ttggctttcg agaaggcgat cgaagccggg
     1321 gtcgacatga tcgagatgga ctgccaactg actcgcgatg gacatgtcgt ggttttccat
     1381 gatgaccgac tcaaacggac ggccggcgtc agcgggactg tcagaagcaa gtctctggag
     1441 cagctcaagc agctcgatgt cgggcaatgg aagaaaaaat cgttcagagg agaacgcgtc
     1501 cttacactgg aagaggtttt ggcgattatc ggaggcaaag ttgacctttg cctcgacatc
     1561 aaacagttca aagactcaca accagggatc gagatcaaac tcttgtttac tctcagccat
     1621 tacgattatc tggatcaaac cattttttct tcattcaact accagtgcct ggaccgcgtc
     1681 cgggagtttg ctccggaagc gcgcatcggg ataatttacg gtaccggcat caaagaagat
     1741 ccgtttggtg ccgcagagcg actcggcgct ggttcgatcc acgttcaaaa ggaagcggcc
     1801 accagaccct ttttggacaa ggcgtgggaa gccggcctcg acattcatgt gtggacggtc
     1861 aataacgttc atgacatgga aaagttcgcc gccttaggag tccaggggat tgtgtccgat
     1921 tatccggaaa agctttgcaa cccaaagctt ttgcacagat aagattctga ttggcgaacg
     1981 aatatcgcat gaaaagttcc tgatgtgttg gtgagcgtct cttaacctca acaactcttg
     2041 tactttctcg ccgatagcgc ggtttcctcc accttgcagt tgatggaaga cgaggtcagc
     2101 gcgctgtgcg ggtttacgtg gccggagttt cattgttaca tcgcatcgtt caccttccaa
     2161 cccctcattt ttactgtgaa atacttgact tgacacccaa atgagctaaa attagctact
     2221 tcaaaaaggg tccggctgga ttgggggtga tgtgatttat gactggagtt agagtccgag
     2281 aaaacgaatc catcgagagc gccattcgcc gtttcaaaaa gctgtgcgaa aaggcgggca
     2341 tactcgccga actgcgtaaa cgagagcact acgaaaagcc tagcgtcaag cgaaagaaaa
     2401 aagctattgc agccaagaaa agagcgctga gacgcgtgcg tcattccttc tagcgtgatg
     2461 aagtaaggag ccgaaaggag ctaacggaca aagggagaga gatgggactc aaggtcgaaa
     2521 tccaggagtc aatgaaagcg gctatgaaaa ctggcgatcg tttgacgctt tcggccttga
     2581 ggttggtcct gtcagcgctg cacaatgagg agataaaggt ccgcagagag ttgaccacgg
     2641 aggaaattca caaaaccata acaactctct gtaaacagcg gactgaagcc attgagctct
     2701 ttagaaaagg cggaagagca gagctcgccg aaaaggaaga agccgagctg aaggttctcc
     2761 aacggttttt gccccagcca cttagtgaag atgaggttcg caacctgatc aaagtctcga
     2821 ttgaggaagt cggagccaaa aatatccagg atctaggtaa ggtcatgaaa cagctgatgc
     2881 caagagtcag tggacgaacc gacggcaagc gcgtcaatga gttagccagg gaacttcttg
     2941 ggggatgaga cgcaatagcc ttgcatgaga taaccccctc tcaaacgagc cgattcagca
     3001 acgcgcctta aagcaaaatg gccatagccg gcaatagcgc gcagttcaaa ccaatagccc
     3061 aagtatcagg acaaacaaac ggttgcagat gtcggtcata agtcagactg acggcatagg
     3121 gtggaatcta ggcggtcgtg tgggatcttt atcctccttg attcaagttg agagcgatct
     3181 ggacatcaat ggtcgacgcc ggtgaactgg attgacctaa tcctcttggt tgtacttttt
     3241 ctctttggcc tgagaggcta tttcaagggg ctgttccgag aaactttatc gctcgccggt
     3301 ctggtcattg gtttcatggt tgcggttcgg tatgatgaag ccgtggctgc ctgggtgggg
     3361 ttctactgga atgtgtcttc gatcattcta aagggtgctg cctttgtcgt cattttcttc
     3421 gttgtgtact ttatctttgc tgttgccggt tggagccttc atcgagccgc aaaagtgctc
     3481 tttttgcaga ccatcaatcg tcttgggggt atcgcggtgg gtctgggcaa aggcacggcc
     3541 gtgacggctc tgatcatctt ctttgtgact tcaagcgcgt ggatccctcg atccacgagg
     3601 gagcgcgttg aagactccta cctcggtcca cctttgtccc agttggcagt gaagctcatt
     3661 cgcgtcggta aagaaaaact gttgcctgaa gagagcggcc gggcgcttat tcagcttaga
     3721 gtgtgttgct cgtaagttgg cgggatgatc aaacaggaaa agatcaccga gattcgcgat
     3781 cgcgcttcca tcgtggaggt gatttcggat tacgtgacgt tgaaaaaagc cggtcggaat
     3841 tatcaggggt tgtgcccttt ccatggggag aaaaccccct cgtttacggt cagtgaagag
     3901 aagggaattt ttcactgctt tggttgtcaa gccggaggaa gcgtttttca tttcctcatg
     3961 aagtacgatc agctttcctt tcccgaggca gtggaacgcg tggccaagcg ctacggcatc
     4021 aagatcgagt catccgatgg cgtgcgggaa cccggcgata gcggcgagcg tgaatcgctg
     4081 tatcgcatta atgagcgcgc ggcggcgaac tatcaccaga tcctgttgag tcagccggcc
     4141 ggccgaaaag cgctggacta tctgaaatcg cgaaaagtga acgaggaaat tgcccagcga
     4201 tttatggtcg gctatgcgcc tcagaccggg tcgggtttgc tggacttgct acgtcgagaa
     4261 aaactctcgg tcaaggatgc aatgcgattg gggttaatcg gccaacagag cgggcaacaa
     4321 ttccatgaaa agttctttgc gcgactgatg ttcccgatta ttaacgcggg aggaaagatt
     4381 gtcggcttcg gcggacgcgt cctcgaccaa gggttgccta agtatatcaa ttcaattgaa
     4441 acaccgttgt ttcgtaaggg ttcgacctta tatggcttgt accaggcgaa agaaggaatc
     4501 cgacgagtcg atcgtgtggt tgtggtggaa gggtatttgg atgttatggc acttcatcag
     4561 tttggtctcg gctatgccgt tgcgaccctg ggaacagctt tgacgccgga tcatgttcgg
     4621 gtcctgggac ggtacacaag gaacattgtg gcgttgtttg atggcgatga tgccggtcga
     4681 aaggcggcgg cacggagttt tgaaatcttt atcgaaaccg gcctgttggg ccgggcggct
     4741 tttttgccga agggcgaaga tcctgatact tatatccatg gccacggtcc ggaggctttg
     4801 gaaaaaatcc tcgacgaagc cgtacccttg gcggactacc acttttcatc cctcacgcaa
     4861 cgattcggaa tgagcttgga aggaaagagc cagattgccg gtgaagtgag tcggctgctc
     4921 acaaaagtca acaatcccat tgatgtggat ttgctagtgc ggcgtgccgt ggatacctta
     4981 ggagtgcggg aggaagtctt gcgccgcccg atcgtgacgc cgggttactc gctacggtct
     5041 aaaatgattc ctaaggcttt gtctgcaacc ccgtccccga ttcgagatga tattgccgaa
     5101 cgctcgttag tgagtttgat gctgcgtttc cctgccgttt tgcgcgcggt ggaaaaggaa
     5161 ggggaagctc gacaatggct cagttcaaag tggcaggcag tgtttgattt gatccttgcc
     5221 gagtggcaag aacgaagaca ggtggatgtt tttcgagtcg ctcagagggt ccatcccgat
     5281 caagcgcccg aaattactgc cttggcattg caaggagaga gcatccttga aacggaaagt
     5341 gacaagatgg cagcggattg cctctcgcat cttcggcgca aatacctaag gacccttgaa
     5401 cgaaatctgc gagttgcaat tcgtgccgcc gaagagcgga aagatgagca agcgaagaga
     5461 gaaaggatac tagaatggca agacgtcgta caaaaagagc gccagctaga acgccaaagg
     5521 ctcgagccga aaacaaccat ccgatagcga cggaaaaatt ggcctccgaa cacgccgggc
     5581 agccggtgga aaggaagaat atgaaggagg tcaaaaagct catcgatttg ggcaaggaaa
     5641 agggctatct gacttatgac gatgtgaacg acatgctgcc agcggaagtg gtctcgccgg
     5701 atcagattga cgatgtcatg tccatctttg gcgaaatgga catcgaagtc gtggatgcca
     5761 accagcgggt gtcgttggga ggcgccggcg aagatcttgt cgaagacgag gaggaagaga
     5821 aggaggtcga gtccgacatt gatggcgata tggtgggcaa gacgggtgac cccgtgcgca
     5881 tgtacttgcg cgaaatggga accgtctctt tgttgagccg ggagggcgag gtcgagattg
     5941 ccaagaaaat cgaacagggt gaaaaacagg tgatcgaaga ggtcctctca agcccgctgg
     6001 cgctgcgcta cgttctggat ctcggcgaaa aacttgcgca acgcgagatt cgggttagag
     6061 aaatcattaa ggacggggac gaggatgctg aatacctcga agacgaggag gttcatgaaa
     6121 agcggctctt agagcaaatg gcaaagattc gtcgcacggc caacgaaacg gaaaagatca
     6181 aaaggcaatt ggatggcaga cgcgtttcaa cgcctcggcg tcagtccctc ctgcaaaatc
     6241 tggctaaata tcaagagaag atcatcggcg gactcaaagc gctgcagctc aatcgtaggc
     6301 agaccgaagc catcgttgat ggtttgaaaa gggccatgga ccggatgcaa acgctggaaa
     6361 gaagggtaaa ggattttgag cagaaaaccg gcaaggcggc gccggatata atcaagcttg
     6421 tttcaggggt aaacggtcgc agaaaggatt ggcagcgcct catcggttcg cttcgcatgg
     6481 gacatgatgc catcgtaacc atggcggaag aaatcaaaaa tgcgcgacgc gacattcgcc
     6541 acatcgaaag ggatctcgag atgccggctg aagaaattaa acgtcgcgtt tggtcgatca
     6601 tcgagggtga gacccgggcc aacttggcca agaaagagct catcgaagcc aaccttaggt
     6661 tagtcgtcag catcgcgaag aaatatacga accgcgggct tcaattcctc gacttgatcc
     6721 aagagggcaa tatcggcttg atgaaggctg tcgacaagtt cgaatatcag cggggttaca
     6781 aattctctac ttatgcgact tggtggatcc gccaagccat cacccgggcc atcgcggacc
     6841 aggctcgaac catccgcatc ccggttcaca tgattgagac gatcaacaaa ttgatccgta
     6901 cttcacgata tctggttcag gaaatcggac gggagccgac accggaagag atcgccagca
     6961 aaatggagat tcccttggac aaggtgcgaa aggttctcaa aatagccaag gagccgattt
     7021 cgctagaaac gcccgtcggg gaagaggaag acagccatct cggcgatttt atcgaagaca
     7081 aacggatcat tacgccctct gacgcggtgg tgaacatcaa ccttcaagag caaacacgaa
     7141 aagtcttggc aacactgacg ccgcgggaag agcaagtact gcgaatgcgt ttcggcatcg
     7201 gggaaaaatc ggatcacacc ctcgaagaag tgggtcagaa gtttaccgtt acgcgcgagc
     7261 gtattcggca gatcgaggcc aaggcgctgc gcaaactcag acatcccagc cgcagcaaac
     7321 ggctcaaaag cttcatggaa agctaacttg gttgtttcga gttgattttc ccggccctca
     7381 accatagagt ctaagctcaa actctagacc ctttttgcta cgggcccata gctcagttgg
     7441 ttagagccac cggctcataa ccggttggtc cctggttcga gtccaggtgg gcccaccatt
     7501 ccccttccgt tgaatttcct actacttgac tctgtttttg ctcttcctct gcctccaagg
     7561 tttcactcaa ggttgcactt ttggccccaa aaaggcggct caactgttcc tgagagtcgt
     7621 tccggatatc cttcccgtaa tgcttgtcga tcatcgccat ggacgttccg cagtattcgg
     7681 ccaaccactg ggattgacgt cattggctaa gcctaccgaa atgaaagtgt ggcgcatcgt
     7741 gtagggcttt cgcacccgta gttcctttcc cctca
//
LOCUS       DASSWZ010000215         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1196452, whole genome shotgun sequence.
ACCESSION   DASSWZ010000215 DASSWZ010000000
VERSION     DASSWZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196452"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFN58_05265"
     CDS             <1..324
                     /locus_tag="VFN58_05265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET8917432.1"
                     /translation="HGGRHLDNTLGTIEVLSEAVAAAKGRTEILLDGGIRRGADVVKA
                     LALGARAVFIGRPLFWGLALGGEQGVIRVLDILREEIEITMAKCGRPTVADIDSTVVV
                     RAPRL"
     gene            516..914
                     /locus_tag="VFN58_05270"
     CDS             516..914
                     /locus_tag="VFN58_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917433.1"
                     /translation="MDKQIVKFRITNLLACVHESIALEWQGKEFDSGPLIIELDKNAL
                     VTGNQGALDYSSSCVRAEFQVRLLFPEFAATLEDLGLDPEFARPVRALIHAEGKILAD
                     HSFSLSGPCDLASHALFIPEETRACVLPGR"
     gene            1115..2221
                     /locus_tag="VFN58_05275"
     CDS             1115..2221
                     /locus_tag="VFN58_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8917434.1"
                     /translation="MLAEPPVLLLTGVTGLVGGELLKLLRVAKPSRRIAALTRHSGKI
                     ADLNSTGGVMALQGDLTYPSLGLDDRTYTQLTGSITEIIHCAADTRFGLSLESARSVN
                     TKGTETLLNLALRCHNLQKFAHISTLYVVGRSTGYFPEGSIHHHSGFFNTYQQSKYEA
                     EELVFQAMNNLPAVVFRLSSIIGNSRTGAVRQFNYVHRLIRLFPQNVLPIAPGLPDAP
                     IDFVASDWAVPALAYLFDSAFVPGRFYNICAGRARSLSLQEMINLTLSIFENHPIGRK
                     WLPIRVPRLVSLSVYEEFVEQRRRDGDKLLNDLFRILEYFLPHLGLVQVFDNGNVTDA
                     LMGSGLEFPPAREYYGKVVQYCLETNWGRRAEAA"
     gene            2406..>3259
                     /locus_tag="VFN58_05280"
     CDS             2406..>3259
                     /locus_tag="VFN58_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917435.1"
                     /translation="MDEAKFFSADSHVNEPPEAWERIPKRLREHGPHFVQDPPGKKGL
                     YMVFDGHEPDPVGMTFTAGQDKSGGGIRKVIETFKWEDWRGPWDPAARIGDMDLDGVK
                     MEVLYPSMARNFYSLKGEEIPLQKAGLVAYNDWMREYCATVPKRLIGLGLLSALDVDW
                     SLEEMKRCAKLGLKGVVLPSQLPDGVSYADPSFDPLWTAAEDMDFPLHFHINIVQGRD
                     RMAARLKVITKLQQGRNAVRRGILEPLNLLTDLVFGGVLERFPRLRFVLAEYDVSWLH
                     PFISKMDGT"
BASE COUNT          709 a          877 c          911 g          762 t
ORIGIN      
        1 catggcggcc gccatctcga taacacttta gggaccatcg aggtgctgtc cgaggcggtg
       61 gctgcggcca aaggccggac ggaaattctg ctcgacggtg gcatacggcg cggcgccgat
      121 gtcgtgaagg ccctggccct cggcgccagg gcggttttca tcggccgtcc gcttttttgg
      181 ggtttggctc tgggtggcga gcaaggcgtg attcgcgtgt tggacattct ccgcgaggag
      241 atcgagatca ccatggccaa gtgtggccgg cccaccgttg ccgacatcga ttccaccgtg
      301 gtcgttaggg cgccacggtt gtaaggcatt catgcatagc gcctcagcaa aaatcaccca
      361 gaatgaaatt cattgtggcc caatgcactc aattgacatg agacgtgttc atgatatcgt
      421 ccgcttaaag cccagaagca cactaccgtc cgaatcctcc ggcgcccggg cgcaaaaagc
      481 aaattcctga aaaccttagc tgaacgggga acatcatgga caagcaaata gtaaagttta
      541 ggatcacgaa tctcttggct tgtgtccacg aaagtatcgc ccttgagtgg cagggcaagg
      601 aattcgattc agggccgctg attattgaac tcgacaaaaa cgccttagtc acaggtaatc
      661 aaggagcgct ggattattcc agtagctgcg tccgtgcgga atttcaagtc cgcctgctgt
      721 ttccagagtt tgcggccact ttagaagatc tcggcctcga tcctgagttt gcccgccccg
      781 tgcgcgcgct gatccacgcg gaaggaaaaa ttctcgcaga tcatagcttc tccctgagcg
      841 ggccctgcga tctcgccagt cacgcgctgt ttatacccga ggaaaccagg gcgtgcgttt
      901 tgcctgggcg ttgacagccc gacatggact cccggtcggg agctaacgtt cggcaagctc
      961 ttcttaacgg ggaagccgtt acctggagat tcgcccctca tcagaaaagg ctcatgttca
     1021 cgctcccgaa gacatttggg gcgcggacct atgaggttag cctaaacggc ggatacactt
     1081 tccatcctgt cgcctccgat tctgcgccag tctgatgctg gcggaaccac ctgtattact
     1141 acttactggc gtcaccggct tggtgggtgg cgagttacta aaactcttgc gtgttgccaa
     1201 gccgagtcgt cgcatcgcag cgctcacgcg gcacagcgga aaaattgccg atctcaattc
     1261 gactggtggt gtgatggctc tccagggaga cctaacgtat ccttctcttg gccttgatga
     1321 ccgcacctat acgcaactga caggcagcat cacggagatt attcactgcg ccgccgatac
     1381 ccggtttggc ctttcactgg agagcgcgcg gtcggttaat acgaaaggta cggaaaccct
     1441 tctaaacctg gctttgaggt gccacaactt acagaagttt gctcatatca gcactctgta
     1501 cgtggtggga cgatcaacag gttatttccc cgagggctct atccatcatc acagcggctt
     1561 tttcaacacc taccagcaat ccaaatacga ggcagaagaa ctcgtgttcc aagccatgaa
     1621 caatcttccc gccgtagtct ttcggctcag ttcaatcata ggaaattcgc gcaccggcgc
     1681 agtgcgacag tttaactatg ttcatcgtct catcaggtta tttccgcaaa acgtgcttcc
     1741 gatagcgcct ggcctgcctg atgcgccgat tgacttcgtc gcaagcgact gggccgttcc
     1801 ggcactggcc tatctatttg actccgcctt tgttcctggc cgcttttata acatctgcgc
     1861 agggcgggcg cgtagtttga gtcttcagga gatgatcaat ctgacacttt cgatattcga
     1921 gaaccatccc atcggccgga agtggctgcc tatacgggtg ccccggttag tatccctttc
     1981 tgtttacgaa gaattcgtcg aacaacgccg tcgcgacggc gacaaattgc tcaatgactt
     2041 gttcaggatt ctagagtact tcttgccaca cttgggcttg gtccaggttt ttgacaacgg
     2101 gaatgtgacg gacgcgctaa tggggagcgg cctggagttt ccaccggcgc gtgagtacta
     2161 cggaaaagtc gtccagtatt gcctggagac caactgggga cggcgcgccg aagcggcctg
     2221 aggtccgcgg gctacgcgct tatgtcctcc ttattaggaa cctggagtgc tttgggtact
     2281 agacggtggt gctaaacctt gagtgcaacc gcacgcttgc gtcgctcgcc atccgcctct
     2341 gcgaagtgag cccaaagaca ggtgacttat gaactcctta aataagtaac gggatgactc
     2401 aaacaatgga tgaggcaaaa tttttctcag cggattctca cgtcaacgag ccgcccgaag
     2461 cttgggagag aatacccaaa cggttgcgtg aacatgggcc tcattttgtc caggatccgc
     2521 caggcaagaa aggtctttac atggtttttg atggtcacga gcccgatccc gtgggcatga
     2581 cctttacggc cggtcaagac aaatccggcg gaggaattcg taaagtgatc gagactttca
     2641 aatgggagga ttggcgtggt ccctgggatc cagcggcgcg catcggcgac atggatttgg
     2701 atggcgtcaa gatggaagtt ctttatccga gcatggcgcg gaatttttac agccttaagg
     2761 gcgaggagat cccgctccag aaggccggtc tggttgccta caacgactgg atgcgtgaat
     2821 actgcgccac cgtgcccaaa cggctcatcg gattaggctt gctctccgca ttggatgttg
     2881 actggtcgct ggaagaaatg aagcggtgcg ccaagctggg gcttaaaggt gtagtcctac
     2941 catcgcagtt gccggatggt gtcagttacg ccgatccgag tttcgaccca ctatggacgg
     3001 cggccgagga tatggatttc ccgcttcact ttcacatcaa tattgtccag gggcgagatc
     3061 ggatggcggc acggcttaaa gtgattacta aacttcaaca aggccgaaac gcggtgcgcc
     3121 ggggcatact cgagcctttg aatcttctga ctgatctggt tttcggcggg gtactcgaac
     3181 gttttccgcg gctgcggttc gtcctcgcgg aatatgacgt ttcctggctg catccgttca
     3241 tcagtaaaat ggacggtac
//
LOCUS       DASSWZ010000216         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_389909, whole genome shotgun sequence.
ACCESSION   DASSWZ010000216 DASSWZ010000000
VERSION     DASSWZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389909"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFN58_05285"
     CDS             <1..582
                     /locus_tag="VFN58_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HET8917436.1"
                     /translation="DSQTEYFNNPGTWYAGRVWQTNFVADVKNLKLLEWKERGGGGSQ
                     VRLEMSENTLCGHVSEFSVGAYKKAHLHGPGAHVIILSGQGYSLLWPKGQPIKRYDWR
                     PGSLVVPPAHWFHQHFNSGAEPVRYLALRWGSQKYHEMWGEGRGKSDVDVKLGGNQIE
                     YEDEDPIVRQMFEEACAKAGIKSLMDKYYRKPA"
     gene            complement(650..2032)
                     /locus_tag="VFN58_05290"
     CDS             complement(650..2032)
                     /locus_tag="VFN58_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8917437.1"
                     /translation="MQHLDSIVADIIVIGAGGCGMMAALVAAKKGAQVLVLEKTDKPG
                     GGTAFSSKGIRAAGSRCQREAKIPDSPELYAEDILRRNNHESDVVLTRRLAEISGRVA
                     DFLADEAGIEFQLGEFPFGHSARRAHSWKADKTITDFLFEAVQRERNIQVCFSTPVLS
                     LRQEANGAVTGVVTVDRVLTGRRVILASGGFGASPELLSQYIPKAADIPFPGHYGSTG
                     DGIKMGLAVGAATENMGAFQPYPAYVGPGKRAVSPEVAVSGGIMVDLGGKRFIDETKY
                     PGGLGAKMLDLPGKQAYEIFDERIYQLHRNLPGLRNLAGLFESGVLKKAQTPEELAGQ
                     LGIDGSGLKQTIQDYNSASSSGKDAFGRALSEPFNAPFYGIKVTVALFHTQGGLRVTP
                     DGQVLRPDGSIVPNLYAGGGVATGISGKGLEGYLPGNGLLASLGLGMRAGEHAVESLK
                     LAVSPASQDP"
     gene            complement(2223..>2765)
                     /locus_tag="VFN58_05295"
     CDS             complement(2223..>2765)
                     /locus_tag="VFN58_05295"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917438.1"
                     /translation="TEGLWKANAARMLENLADYSHFPFVHPGTLGDPDNPECEPILIE
                     PLDGGFLYDIPQPVNRLRPDAGARQTYRLILPFMLMIQRWQPGGNERQTNIFLCSPIS
                     KNETRFFRFMGRNFVGHFSDEELQHRHRLTFEQDRSIVESQRPEELPLDLSEELHLRG
                     PDTPAIEYRRRLRELGIDWN"
BASE COUNT          600 a          803 c          771 g          591 t
ORIGIN      
        1 gactcccaga cggagtactt taacaatccc ggcacctggt acgcgggcag ggtctggcag
       61 acgaactttg tcgccgatgt aaaaaaccta aagttgcttg agtggaagga gaggggaggc
      121 ggcggctccc aggtgcgctt agagatgtct gaaaacactt tgtgcggtca tgtgtctgaa
      181 ttttccgtgg gcgcatacaa aaaagcgcac cttcacgggc cgggagctca cgtcatcatc
      241 ctaagcggcc agggctactc tctcctgtgg cccaagggac agccgattaa acgctatgat
      301 tggcgccctg ggagcctggt tgttcctccg gcgcactggt ttcaccagca cttcaattca
      361 ggggcggagc ccgtgcgcta tttggctcta cgctggggca gccagaagta tcacgagatg
      421 tggggggaag ggagaggcaa gagcgatgtg gacgtgaagt taggcggcaa ccagatagaa
      481 tatgaagacg aagaccccat cgtgcggcag atgtttgagg aggcttgtgc caaggctggt
      541 atcaagagcc taatggataa atattatagg aagccggcat aggttagtcc attgccttcg
      601 atccggaggg ctcgtccctt gatcgttact tgccagtgaa taatccgggc tatggatctt
      661 gcgaagccgg actaaccgcc aactttaagg actcgactgc gtgctctccg gctctcatgc
      721 ctagacccag agaagccagt aacccgttac ctggtagata gccctccaaa cccttgccgg
      781 agataccagt agctacgcca ccaccggcgt acagattggg gacaatcgag ccatcggggc
      841 gcaggacctg tccgtccggg gttactctca gaccaccttg agtgtggaac agcgccaccg
      901 tcaccttgat tccgtagaat ggggcattga acggttccga cagcgcgcgc ccaaaagcgt
      961 ctttgccgct gctcgaagcg ctgttgtagt cctggatggt ctgcttcagg ccgctgccat
     1021 cgattcccaa ttggcccgcg agctcctccg gagtctgcgc ctttttcaaa actcccgact
     1081 caaacaagcc tgccaaattt cttagtcccg ggaggtttcg atgcaactgg tagatacgct
     1141 cgtcgaagat ttcgtacgcc tgctttccgg gaaggtccag catcttagcg ccgagtccgc
     1201 cgggatattt agtctcgtca ataaagcgct tcccgccaag gtcgaccatg atcccgccgg
     1261 acacagccac ctcgggcgac accgcgcgct tccccggacc cacgtaggcg ggatagggct
     1321 ggaaggcgcc catgttctcc gtagctgctc ccaccgccaa acccattttg atcccatccc
     1381 cggtgctccc gtagtgacca ggaaagggaa tatccgctgc tttgggaata tactgggaca
     1441 acagctctgg gcttgcccca aatccaccgg aggccagaat aaccctacgc cccgtgagga
     1501 ccctatccac ggtcaccacg cccgtcaccg cgccgttcgc ctcctgtctg agcgaaagca
     1561 ccggtgtcga gaaacacacc tggatgttcc tttcacgctg aactgcctcg aagaggaagt
     1621 cggtgatggt tttgtcagcc ttccacgagt gggctcgccg tgcgctgtgg ccaaagggaa
     1681 actcgccgag ctggaactca atgcccgcct catcagccag gaaatcagcc acgcgcccgg
     1741 agatttccgc caggcgcctg gttaggacga cgtcgctctc atggttgttc ctacgcagga
     1801 tatcctctgc atacagctcc gggctgtccg gaattttcgc ctcgcgctga catcgacttc
     1861 ccgcagcgcg gatgcctttg gaagagaaag ccgtcccgcc tcccggtttg tccgtctttt
     1921 caaggaccag gacctgcgcg cccttcttgg ccgccaccag agcggccatc atgccgcatc
     1981 cccctgcacc gatgacaatg atatcagcaa cgatcgagtc taagtgctgc accggacctc
     2041 caaaaagggc tgcctattga tgtatggaaa aatgttaagc gcataagagt agggattaac
     2101 tctaatccga cattatttct aagctgctgg ggcgatgagt cctacagccg tgggcaatgg
     2161 gacactgcct agagggccac cccgcaatcc ctaagggtga tgtttcgtgc agtcagtgaa
     2221 aactaattcc agtcgatacc caactctcgc agccgtcgcc gatactctat ggccggtgta
     2281 tccggaccac gaaggtgcaa ctcttcactg aggtcaagcg gcaactcctc cggccgctga
     2341 gattccacga tacttcgatc ctgttcaaag gtgaggcgat gcctgtgttg cagctcctcg
     2401 tcggaaaagt gaccgacaaa gttgcggccc atgaatctga agaaccttgt ttcgttcttc
     2461 gatatcgggc tgcaaagaaa aatgttggtc tgacgttcgt tgccgcctgg ctgccatctc
     2521 tggatcatca gcatgaatgg aagtatgagc cgatatgttt gtctggcccc ggcgtcgggc
     2581 ctcagccggt ttacgggttg cgggatgtca tagagaaatc cgccgtccag gggttctatc
     2641 aagataggct cgcattccgg gttatcggga tcgcccagag tgcccggatg aacgaaagga
     2701 aaatgtgaat aatcggccag attctcgagc atgcgcgccg cgttcgcttt ccacaggccc
     2761 tcggt
//
LOCUS       DASSWZ010000217         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1210748, whole genome shotgun sequence.
ACCESSION   DASSWZ010000217 DASSWZ010000000
VERSION     DASSWZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210748"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFN58_05300"
     CDS             <1..593
                     /locus_tag="VFN58_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8917439.1"
                     /translation="FSRRREPVFQPIDLLALLDNVKYLLAHQLQEKGIRVEMKGLDVL
                     PRISADPELLQQIFINLYSNSLHALGLGGVIKIAAEITDRGLTRALPPAGHWLRISFE
                     DNGAGIGAEHIGRVFDPFFTTKDVGEGIGLGLSVSYGIIQEHGGDIHVESEPGRFTCF
                     MIYLPTEQPKQRSYSNNSYDSRVQAANHQSGQDSRR"
     gene            538..1926
                     /locus_tag="VFN58_05305"
     CDS             538..1926
                     /locus_tag="VFN58_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET8917440.1"
                     /translation="MTPEFKQQTINRARILVVDDDREMCQFLADLLGEEGYLVETVHD
                     GPSAVERYRAGSFDLTITDLMMPRMRGTELVRQLREIDAHASVLLITAFGSIESAVEA
                     MHAGAFHYVTKPFRTDEILIQVTRALEQHSLQSEVERLRQQVHRHYGFENIIGQSAKM
                     REIFDLVTHVSDLAVNVLIVGESGTGKEMIARAIHQTSARAARSFIPINCAAIPETLL
                     ESELFGYVRGAFTDARKDRRGLFQAASGGVLFLDEISEIPLGLQAKLLRVIEDKEVRP
                     LGANQGEKVDARLVSACNRDPELLVQEGRFRQDLYYRLNVIRIDLPPLRERAEDIPVL
                     IEHFIRKFGDQVQRKLDGIEPEALTALMNYHWPGNVRELEHTIERAVLLGKEGRIGPQ
                     DFPPSLVASKDNVSPLADAVARSYTLRDLEKEYIIRVMEIVHGNKTEAAKTLGVDRTT
                     LYRKLEEYKVKD"
     gene            complement(1916..2785)
                     /gene="rsmI"
                     /locus_tag="VFN58_05310"
     CDS             complement(1916..2785)
                     /gene="rsmI"
                     /locus_tag="VFN58_05310"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET8917441.1"
                     /translation="MLVTSVVLSLSMPGMLYIVATPIGNLEDITLRALRVLKEVDVIA
                     AEDTRHTQILLSHHDIHTPLTSYHEHNEKTKAQDLVARLARGQNIALVSDAGTPAISD
                     PGFRLVVQAIRAGVRIIPIPGASALTAVLSATGLPTDRFVFEGFLPAKKKQRRERLQI
                     LRDEIRTLIFYEAPHRLRDTLEDIHELLGNREAVLAREVSKIHEEFLRGPISELVRAL
                     GGGDVRGEVTLIISGSAGESRVNEDLLKAEIRELKRQGLRVKEIAEVLGEKFGYPKKD
                     IYRLALHGEKVSP"
     gene            2957..3172
                     /locus_tag="VFN58_05315"
     CDS             2957..3172
                     /locus_tag="VFN58_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HET8917442.1"
                     /translation="MIEEVLIFGHDLCPYTSRAREDFARRKIPFNYVNVLQDNDGLQR
                     MLKHSNGRRQVPVIVEAGTVTIGFGGS"
BASE COUNT          796 a          919 c          928 g          745 t
ORIGIN      
        1 aattttcacg gcgcagagaa ccagtttttc agcccattga tctcttggct ctcttggata
       61 acgtaaaata tcttttggca catcaactcc aagagaaagg tatccgagtt gaaatgaaag
      121 gcctcgatgt cctgcctagg atcagtgctg atcctgaact gcttcagcaa atatttatca
      181 atctttattc aaactcgctc cacgcattgg gcctgggtgg cgtgatcaag atcgccgcgg
      241 aaatcaccga tcgcggtctc actagggctt tgcccccagc cggtcattgg ctgcgaatct
      301 cattcgaaga taacggtgcc gggatcggcg ccgagcatat cgggcgcgtg tttgatccat
      361 ttttcacgac taaggacgtc ggcgagggaa tcggcctggg tttatccgtg agttacggca
      421 tcatccagga gcatggcggt gatattcacg tcgaaagcga gcctggccgt tttacctgct
      481 ttatgattta tcttccgaca gaacaaccca agcagaggtc ttacagcaat aactcctatg
      541 actccagagt tcaagcagca aaccatcaat cgggccagga ttctcgtcgt tgacgacgat
      601 cgcgagatgt gccagttcct tgccgattta ttgggcgagg agggctatct agtcgaaacg
      661 gttcacgatg gcccgtcggc ggtggaaaga taccgagcgg gcagctttga tttaaccatt
      721 accgacctca tgatgccgcg catgagggga accgaactgg ttaggcagct tagagagatc
      781 gatgcccatg cttcggttct tctgattacg gccttcggga gcatcgaaag cgctgtggaa
      841 gccatgcacg caggagcgtt ccactatgtg accaagccgt tccgcacaga tgaaatcctt
      901 attcaggtaa cccgcgctct agagcaacac agcttgcaga gtgaggttga aaggctgcgc
      961 caacaagtcc accgtcacta cggattcgag aatatcatcg gtcagagcgc aaaaatgcgg
     1021 gagatattcg atcttgtcac tcacgtgagc gatctcgctg tcaatgtact tatcgtgggt
     1081 gaaagcggca ccggcaaaga aatgatcgcg cgtgcgattc atcaaaccag cgctcgggcg
     1141 gcaagatcct ttattccgat caactgcgcc gccattccgg agacgctttt ggagagcgaa
     1201 ctcttcggtt acgtgcgcgg cgcttttacc gacgcgcgga aagatcggcg cggtctgttt
     1261 caggcggcta gcggcggcgt gctgtttctc gacgagatca gcgagattcc gcttggccta
     1321 caggccaaac tgctgcgcgt tatcgaggat aaagaagtga gaccgcttgg agcaaaccaa
     1381 ggtgagaaag tggacgcccg gttggtttca gcgtgtaacc gcgatccaga gcttttggtc
     1441 caggaaggcc gcttcaggca ggacctctac taccggctca acgtaatccg aatcgatctg
     1501 ccaccccttc gcgagagagc ggaagacatt ccggttctga tcgagcactt catacgaaaa
     1561 tttggcgacc aggtccaacg taaattggac ggcatcgagc cggaagccct taccgctctc
     1621 atgaactacc actggccggg aaacgtcaga gagctggagc acaccatcga aagggcggtg
     1681 ctcctcggca aagagggccg gattggtccg caggactttc cgccttcgtt ggtcgcgagc
     1741 aaggataatg tgtcgccgct cgccgatgcc gttgcaagat cctacacgct cagggacttg
     1801 gaaaaagaat atatcatacg cgtcatggaa atcgtgcacg ggaacaaaac cgaagccgct
     1861 aaaactctcg gtgtggaccg gaccaccttg tatcgcaaac tggaagagta caaggtcaag
     1921 gactgacctt ttctccatgc agtgccaagc gataaatgtc ctttttgggg taaccgaatt
     1981 tttcgcccaa gacctcagcg atttccttaa cgcgcaaccc ttggcgtttg agttcacgga
     2041 tctcagcttt caaaagatcc tcattaacac gggattcacc tgctgagccg ctgatgatga
     2101 gcgtaacttc tccgcgtacg tcgccgccgc cgagcgcgcg gaccagctcg cttatgggtc
     2161 cgcgcaggaa ttcctcatgg attttgctca cctctcgagc cagcaccgct tcgcgatttc
     2221 ccagcaactc gtgaatgtcc tcgagagtgt ccctcagccg atgcggagcc tcgtaaaaga
     2281 ttaacgtcct gatctcatcg cgcagaatct ggagtctttc tcgccgctgc ttttttttgg
     2341 ctggcagaaa cccctcaaac acgaatcgat ctgtaggtaa gccagttgcg ctgagaacag
     2401 cggtcaaggc tgaagcgccg gggatcggaa tgatccgaac cccggcacgg atcgcttgta
     2461 caactaatcg aaagcccgga tcggagatcg ctggcgtgcc cgcatcagac accaaggcaa
     2521 tattctgacc ccgcgcaagc cgggcaacca agtcctgggc tttggttttc tcgttgtgct
     2581 catgatagct ggtcagcggt gtatgaatat cgtgatgact cagaagaatc tgcgtgtgac
     2641 gcgtgtcctc ggcggcgatc acgtcgacct ctttaagcac ccgcagagcc cgcaaggtaa
     2701 tgtcttcgag attaccgatg ggggtggcaa cgatatagag catgcccggc atggaaagag
     2761 atagaaccac actcgtcact aacaccgcgg atcatcctca cgatcccatg gcgtcggatg
     2821 aaccgagcgc aggaacagga ttctcagcag gctaacacag caaaatagtt ccttgcaatt
     2881 taccgcccaa cagctaagtt agagttgttt gaatcgctaa tcgaattgag ccggaacggg
     2941 tcgacaggag ccgcagatga ttgaggaagt gctgattttc ggtcacgatc tttgccctta
     3001 cacttcaaga gcccgtgagg acttcgcgcg tcggaagatc cccttcaatt acgtcaatgt
     3061 ccttcaagac aatgacgggc tacagcggat gctgaagcat tccaacgggc gccggcaggt
     3121 accggtcatt gtggaagccg gcacggtcac gatcggcttt ggcggcagtt gagaggttta
     3181 agaaggccga tggcgtcggc ctgcatcaaa gctctgtttg ccggctgctc aggttcacgc
     3241 cttgatcaat ctcaggtgcg cgatctgcgg cgggcaattg atcctgatcg gcaccccggt
     3301 gattcctatg ccacgactga cgaacaggtg cgcggttttc ccagcgccgt ggcccgccgg
     3361 cgcaagccgg aaaagcccct cagcataa
//
LOCUS       DASSWZ010000218         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_22085, whole genome shotgun sequence.
ACCESSION   DASSWZ010000218 DASSWZ010000000
VERSION     DASSWZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22085"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFN58_05320"
     CDS             <1..734
                     /locus_tag="VFN58_05320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8917443.1"
                     /translation="YAYIEKELHQYYQKHFPRCLPVCVSHAQANMLGDDSIPVVYNAV
                     DLGEIPFNDRPEDFFMIVGRMTPGKGIAEAIRIAKKAKVKLLIVGHVTTHLPWSEEYF
                     LKEIKPHIDGDRIRYIERMAYHDIVETMGKAKGFLFPLQWDEPFGMVVIEAMAAGTPV
                     LTYGRGSMPELIQNGETGYLVENEEEMVEMTHRVASVDRARCRSWVQQRFSVEQMVQG
                     YEKLYRRATHGHWNRKGDAERRHAR"
     gene            724..1728
                     /locus_tag="VFN58_05325"
     CDS             724..1728
                     /locus_tag="VFN58_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET8917444.1"
                     /translation="MPARAWNLKRLRSESSYWRSCILITAAHLDLFGWIGKQEKSPRA
                     MTTHFGGSAAGWEIFLNALCSMGLMQKRGGKYANGAFAARCLSRGGATSLLPAYDAWN
                     IWGGLASALTLGKRPAMQRPFVSDRSKAKRLLHSLDVDARDIATYLIKKLPLSRSRTL
                     LDVGGGLGTFSVAFCRHYPNLRATLVEHPHVLPLARRAIVESGMARKVHIVGADFSRD
                     ALPQGFDTVFVSNVLHAHGVGVNHSLLRKLQRCLNPHGQLIIRDVFMNRQRTAPEWST
                     LFSVLLLLHTPQGRCYALNEIRTWLRQAGFSRIRGPFRSSPLPFDPDSVLIATKTDRK
                     "
     gene            complement(1787..1972)
                     /locus_tag="VFN58_05330"
     CDS             complement(1787..1972)
                     /locus_tag="VFN58_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019274801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET8917445.1"
                     /translation="MPVVHIHMYKGRTKEQKKELVRRITEDFEKVVNVKPESLHILFQ
                     DIDKEDWGTRGNLASDL"
     gene            complement(2060..>2782)
                     /locus_tag="VFN58_05335"
     CDS             complement(2060..>2782)
                     /locus_tag="VFN58_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917446.1"
                     /translation="GTLALRAPVDIRMAIGFSTAKEDLKLEQVYRVDGYSVYYRQGNE
                     FADSRSVATAGFAGEGTVRRVKVILHEDLHGDQNFDLAWEVEESIVTPLGSLAAVEFF
                     SSRGDQEHLKQALASLEEERQLARELNALVAKAEKLFRTTTIEEAKTQILDSIPSYPV
                     YHRQFQRQIADQHPPTVLEAKLSHDLAYYRYFDQIAALSEKVADLRTLIADFKKLPHD
                     TSQDGMQAYLQQLKTKYSQSAK"
BASE COUNT          613 a          753 c          755 g          661 t
ORIGIN      
        1 cctacgctta tatcgagaag gaactgcacc aatattatca gaaacacttt ccacgttgcc
       61 ttccggtatg cgtcagccac gctcaagcaa acatgctggg agacgattcg attccggttg
      121 tttataacgc ggtggatcta ggtgaaattc cttttaatga ccggccggag gatttcttca
      181 tgatcgtcgg ccgcatgacc cccgggaagg gcattgccga ggcgatccgc attgccaaga
      241 aggccaaagt gaagctgctc attgtcggcc acgttaccac ccacctaccc tggagcgagg
      301 agtacttttt aaaggaaatc aagccacaca tcgacgggga tcgcattcgt tatatcgagc
      361 gcatggccta tcacgacatc gttgaaacga tggggaaggc caaaggcttt ctctttccgc
      421 tgcagtggga tgagcctttt ggcatggtgg tcatcgaagc gatggctgcg ggaacgccgg
      481 tgctgacgta cggccgcggc tcgatgcccg aattgatcca gaacggcgag accggctatc
      541 tggtggaaaa tgaggaagag atggtcgaga tgacacatcg ggtcgcaagc gtggatcgcg
      601 cacgctgtcg gagctgggtg cagcagcgtt tttctgtaga gcaaatggtg cagggctacg
      661 agaaacttta tcgaagggcg acccacggac actggaaccg gaagggcgac gcggagcggc
      721 gtcatgcccg ctagagcatg gaatctcaag cggctacgga gcgagagcag ctattggcgc
      781 tcgtgcattc tgatcacagc ggctcatctc gatctattcg gttggatcgg caagcaagaa
      841 aaaagcccca gagctatgac gactcatttc ggcggcagtg ccgccggatg ggagatcttt
      901 ctcaatgcgc tctgcagcat gggtttaatg caaaagcgcg gcggcaagta tgccaatggt
      961 gctttcgctg cccgttgtct cagccggggc ggcgcgactt ccctactgcc cgcttatgac
     1021 gcctggaaca tttggggcgg actagcctct gcccttaccc tgggaaagcg acccgcgatg
     1081 cagcggcctt tcgtttccga tcggagcaaa gcgaagcggt tgcttcatag tctggatgtc
     1141 gacgctcggg atatcgcaac ctatcttatc aagaaattac cattgagccg ctcgagaaca
     1201 ctgttggatg tcggcggcgg gctgggaact ttttccgttg cgttttgtcg acactatccg
     1261 aacctgcgag cgactctcgt cgaacatcct cacgttcttc ctctggcgcg tcgggctatc
     1321 gtagaatccg gtatggcaag aaaagtgcac atagtcgggg cggatttctc acgggatgct
     1381 ctgccacagg gattcgatac ggtatttgtc tccaacgtcc tgcacgccca cggcgtcggt
     1441 gtgaaccact ccctactacg taagctccag cgctgtttga acccccacgg ccaattgatc
     1501 atccgtgacg tattcatgaa ccggcagcgc acggcacccg agtggagcac gctcttttcg
     1561 gtcctgctac ttttgcatac tccacaagga cgctgctatg cgctcaatga gatccgcaca
     1621 tggctgcgcc aagccggctt ttctcggata agggggcctt ttcgctcgag cccacttccg
     1681 tttgatccag actcggtctt aatcgcgaca aagacggata ggaagtgacg agagcaactg
     1741 agactaactg gcctcatctc actactctca ttgcgattcc gcttgtctaa agatctgaag
     1801 ccagatttcc ccgggtgccc caatcttcct tatcgatatc ttgaaagagg atgtggagcg
     1861 actcgggctt gacattaact accttctcga agtcttcagt gatccggcgc accagttcct
     1921 tcttttgctc cttggttctt cctttgtaca tatgaatgtg taccaccggc atattcacct
     1981 ccgttattga aaggataaat gcaagcatta tgagagcgaa aggcataagg caagaggcgc
     2041 agacagctag cccccgtttc tatttcgccg actggctgta tttagttttc aattgctgaa
     2101 gatacgcttg cattccatct tgggaagtat catggggaag ttttttgaaa tcagcgatga
     2161 gagttcgtag gtcggcgact ttctccgaca aagccgcaat ctgatcgaaa tatcggtagt
     2221 acgccaggtc atggctcagc tttgcttcca gcacagtggg tgggtgctgg tccgctatct
     2281 ggcgttgaaa ctggcggtga taaactggat aagatggaat tgagtccaga atctgtgtct
     2341 tggcctcctc tatcgtagtg gttctgaaaa gcttctccgc cttggccacc agagcattca
     2401 actcccgcgc caactgtctc tcttcctcca gcgatgccaa cgcttgcttt aagtgttcct
     2461 gatctcccct gctgctgaaa aattccaccg cggcaagaga gccgagcggc gtgacaatcg
     2521 actcttcaac ttcccaggct aaatcaaaat tctggtcgcc gtgcaggtcc tcgtgcagaa
     2581 tcactttgac gcgtcgaacg gtgccctcgc cagcgaaacc cgccgtggcg acggaacggg
     2641 aatcggcaaa ttcatttccc tgtcgatagt agacgctata tccgtctacc ctgtagacct
     2701 gttccagttt cagatcctct ttggcggtgg agaagcctat ggccatgcgg atgtcgacgg
     2761 gcgctcggag cgccaaagtt cc
//
LOCUS       DASSWZ010000219         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1140026, whole genome shotgun sequence.
ACCESSION   DASSWZ010000219 DASSWZ010000000
VERSION     DASSWZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140026"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1159)
                     /locus_tag="VFN58_05340"
     CDS             complement(182..1159)
                     /locus_tag="VFN58_05340"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917447.1"
                     /translation="MNRLLYVAVFFVATGSVAFWSPAHAQIRHLNICVTDYSVGVMPL
                     ILAKRNGYFAQEQLEVDLLAARGSLCTMGLIGGSFQATYSPSTFDAVVAGDVKGKVIY
                     VAEKFLLHRLIVAPQIKRFEDLKGKKIAISSFGSLTDLLSREILADHGLRAMQDVLLL
                     QTGGAPVRLAALKSGNVQGALLSSQHALAAMQDGFRNMEYEPPPYVSHPLIVKNEMLT
                     GEKALVRSLLQAMLKGHIFFGQKKEETLTAIQKTLRIDDRKVARETYEDEMRRYNPGG
                     GFEATKMTKVMDRARDTRKMERKVEIQDVFDLSMAAELDAGLKKMGWKP"
     gene            complement(1278..>2054)
                     /locus_tag="VFN58_05345"
     CDS             complement(1278..>2054)
                     /locus_tag="VFN58_05345"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917448.1"
                     /translation="QICTMALINGQMELTTNPNVFDAMVAGKFQGKVIYVTAKTLGHR
                     FIAAPDIKGFNDLKGKTIAISTFGGLTDMLTREILEQHGIRPHKDVALLQLGTPDLRY
                     GALKAGTVKAALVSSTQALTGLRDGFRELRYEQPPWLSSPIVGSDELLARDRAMLRGF
                     VRAVTKGHLYYGHYPSQAIALTQKVQRIDSQAMAKQIYDDDMLRHNPGGGLEETAMRK
                     VVERSREMLKVQRKVELVDIFDLSMAKEAEAELKKAKWEP"
BASE COUNT          438 a          607 c          497 g          512 t
ORIGIN      
        1 ctccggcaga taggcccatg gcatcaaatg aaagcaataa acttgcatat tagtcctaac
       61 agcgtcctta aaaaccttgt ctacaggctg cagtcgcctt agggcgtatg gcctatagcg
      121 tatggttcct cggttctatc cagacgccat aggctatcag tcacatgcca actaacgtct
      181 gctacggttt ccatcccatt tttttgagtc cggcatccag ttccgcagcc atactcaaat
      241 cgaagacgtc ttgaatctcc actttccgtt ccatctttct cgtatcacgc gctcgatcca
      301 tcactttggt cattttggtc gcctcgaagc caccgccggg attgtaccgg cgcatctcat
      361 cttcataggt ttctcgcgcg actttgcggt cgtcgatgcg caaggttttc tgtatggcgg
      421 tgagcgtctc ctccttcttc tggccgaaaa atatatggcc cttgagcatc gcttgaagca
      481 gtgatcgcac caacgctttc tcgccggtca acatttcgtt cttgacgatc aacggatggg
      541 acacataggg aggcggctcg tactccatgt tacgaaaacc gtcctgcatc gccgccaggg
      601 catgctggga ggacaaaagc gctccctgca cgttgcccga tttcaacgcg gctagtcgta
      661 ccggcgcccc gccggtctgg agcagcaaaa cgtcctgcat cgccctcagc ccgtggtcgg
      721 cgagaatctc tcgactcaac aaatcggtca agctaccgaa cgagctgata gcgatctttt
      781 ttcctttgag atcttcaaat ctcttgattt gcggcgccac gatcaaacga tggagcaaga
      841 atttttccgc cacgtaaatg acctttccct tgacgtcgcc ggcaaccacc gcatcgaagg
      901 tgctgggcga ataagtggct tgaaaggaac ctccgataag ccccatcgtg caaagactac
      961 cgcgtgccgc gagcaagtcg acttccagtt gctcttgtgc gaagtagcca ttccgcttgg
     1021 cgagaatcag cggcatgacg cccacagagt aatccgttac acaaatgttc aaatggcgta
     1081 tttgagcatg agccggactc cagaaagcaa cactgcctgt ggcaacgaag aaaactgcga
     1141 catacagcaa acgattcatt caatactcct tcctcgtgtc atcttcgagc ttaccacggc
     1201 gcgctcagtt tgccctcaag ctttggacac aactgttctt gtgctctgct ttttttggag
     1261 tcaatccatt ctttatccta tggctcccat ttggctttct ttaattccgc ttctgcctct
     1321 ttggccatgc tcaaatcaaa aatatccacg agctcgacct tcctctggac tttcaacatt
     1381 tcacgagagc gctccaccac tttccgcatg gcggtttcct ccagtccccc gcccggattg
     1441 tgccggagca tgtcatcgtc gtagatttgc ttcgccatcg cttgactatc tattcgctgc
     1501 accttctggg tcagtgcgat ggcctgactt ggatagtgcc cgtaatatag atgacccttg
     1561 gtcaccgccc gcacaaaccc gcgtaacatc gcgcgatctc gagccaacag ctcgtcgctc
     1621 cccacaatgg gcgaggaaag ccacggcggt tgctcatagc gcagctcgcg gaagccatcc
     1681 cggagacccg tcaatgcttg ggttgacgaa accagcgccg ctttcaccgt gccggctttc
     1741 aacgctccat accggagatc gggcgtgccc agttgcagca aggcaacatc cttgtgcggt
     1801 ctaatcccgt gctgttcgag aatctctcgg gtgagcatat ccgtcagccc gccgaaggtg
     1861 ctgatggcga tggtcttgcc cttgaggtcg ttgaagcctt tgatatcggg agccgcgata
     1921 aatcgatggc cgagagtttt agccgtgaca taaatcactt tgccctggaa cttgccggcg
     1981 accatggcgt cgaagacatt gggattggtg gtaagctcca tttgcccgtt gatcagcgcc
     2041 atggtgcaga tctg
//
LOCUS       DASSWZ010000220         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1408852, whole genome shotgun sequence.
ACCESSION   DASSWZ010000220 DASSWZ010000000
VERSION     DASSWZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408852"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..971
                     /locus_tag="VFN58_05350"
     CDS             69..971
                     /locus_tag="VFN58_05350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET8917449.1"
                     /translation="MNSSSMEVGPIGIWTAQFDYQPAAKAREAAAELEQLGFGTIWFP
                     ESVGRESLTHAALLLGATSRIVIATGIANIYARDPVTTATGQNTLAEAYPGRFLLGLG
                     VSHIPLVEQIRGHSYGRPLASMRAYLDGMDRAPYRAIQPSVKPVRVLAALGPGMLRLA
                     AERTGGAHSYFVPPEHTARAREILGNDRLLAVEQAVVLETDSTKAREIARAHTARYLA
                     LPNYVNNLRRLGFGDEDIANGGSDRLVDAIVAWGDLSAVTDRVRAHQSAGADHVCVQV
                     LPRDPQALPMPEWREVASALQKSK"
     gene            complement(1216..1725)
                     /locus_tag="VFN58_05355"
     CDS             complement(1216..1725)
                     /locus_tag="VFN58_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917450.1"
                     /translation="MKGINARTTLKLVLSVSWLIALAVVGPGYAGDYYIYQDPNGKLV
                     LSNYAPPQGSNVIKKETLPEVTDQQIIESRLRENQLAVDNRLSSLERSVGELAENLRA
                     QSEVITNVPEGYGDTNIAVGVVQGPAIVARPPHKNFNHPGNLRRNLPKVQPRPVAPAA
                     RSGGGGRAG"
BASE COUNT          458 a          587 c          600 g          457 t
ORIGIN      
        1 gagtatttac tatcggcatc gcgagctgca acaatcccta agtcatgccc gaacgaaagg
       61 agcaaccaat gaactcctcg tccatggaag tgggaccgat tggaatctgg acggcgcagt
      121 ttgattatca gcctgccgcc aaggcgcgag aagctgccgc ggaactagag cagctcgggt
      181 tcggaacgat ctggttccca gaaagtgtgg gtcgcgaatc gctgactcat gcggcgttac
      241 ttctcggcgc aaccagccgc atcgtcattg ccacagggat tgcaaatatt tacgcccgcg
      301 atccggtcac gacggccacc ggtcagaata ccctggctga agcctaccca gggcggttcc
      361 ttctcggtct cggtgtcagc cacattccgc tggtggaaca gatacgaggc catagttacg
      421 gtagacccct cgcttcgatg cgggcatacc tggatggaat ggaccgggcc ccttaccggg
      481 cgattcagcc gagcgtgaag cccgtcagag tcttggccgc acttggacct ggaatgctgc
      541 ggttagccgc ggagcgaacg ggcggggcac attcgtactt cgtccctccg gaacacaccg
      601 ctcgcgcccg tgagatccta ggcaacgatc gacttctggc ggtggagcag gccgtcgtac
      661 tcgaaaccga ttcaactaaa gcgcgagaga tcgcgcgagc gcataccgcg aggtatctag
      721 cgttgcctaa ctatgtgaat aacctccgtc gtctcggctt cggtgatgag gacatcgcaa
      781 acggtggtag tgaccggcta gtggacgcca tcgtcgcctg gggtgatttg tctgctgtta
      841 cggatcgagt tcgggcccat cagtccgcgg gcgccgacca cgtctgcgtt caggtgctgc
      901 cacgggaccc gcaggcgctc ccaatgccgg agtggcgcga agttgcatcc gccttgcaga
      961 agtccaagtg aaacggaaac cgaaggtcct gacacttagc cttgtttgac aactacccgg
     1021 agcaaggtca gtgcgagact gatcgatatt tagaagccac ggaaaacaga ggttaagcgc
     1081 ttgagactct tccgcagagc cgaattggcg agggttcctt gacgagatat cggcgcgcgc
     1141 cgaaatatcg ccaagtgggt cagttaactg gaaaagcatt aagacaagcg gcggggaaca
     1201 aaaacaaaaa gagctttacc ctgcccttcc gccaccaccg gaacgcgccg caggcgccac
     1261 agggcgaggc tggactttgg gtaaattgcg ccgcaaattt ccagggtggt tgaaattctt
     1321 gtgcggtggc cttgctacta ttgcgggtcc ttggaccacg ccaaccgcga tattggtgtc
     1381 gccgtaaccc tccggcacgt tagttatgac ttcgctttgc gcgcgcaaat tttcggctaa
     1441 ctcgccgaca ctcctttcca aactcgagag ccgattatca actgcaagct ggttctcacg
     1501 gagccgggac tctatgatct gctgatctgt gacttcggga agcgtttcct tctttataac
     1561 gttgctcccc tgaggcggcg cgtagtttga gagaaccaac ttgccgttgg ggtcctggta
     1621 tatgtaatag tctccggcgt agcccggccc aaccactgcc agagcaataa gccaggaaac
     1681 agaaagaacc aacttcaaag tcgtgcgagc gttgatgcct ttcatagtta gtggtcgcaa
     1741 actgcggtgc cagctttgct gtgaccccct tgggcttggt cccgtctttt gatgccatgt
     1801 cagtgatcga aagacgggga tcagttttca aactccacca ccaggtaaac tagccgtccg
     1861 gctacttcat gtcaatctcg ggcgaattct cggcaacggt gtgggaacca aggtacgacc
     1921 ggatgccgac tatagttcgt ttaagtgtgg tctttggccg cagctccagc tgtttactta
     1981 tcgcagctgg aacgtatcaa aggtcgtggc ccgcttctgc agaccaactt ttcacttcat
     2041 gaagcacgaa atggcgtcac tgcttttcgc tcagcgggat gtgacagcta gggctccgct
     2101 tt
//
LOCUS       DASSWZ010000221         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_899742, whole genome shotgun sequence.
ACCESSION   DASSWZ010000221 DASSWZ010000000
VERSION     DASSWZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899742"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFN58_05360"
     CDS             complement(<1..752)
                     /locus_tag="VFN58_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917451.1"
                     /translation="MPEPLVRPTASFLNYTPVPLAFGTSGLRGLVKDITDLEAYINVK
                     AALRYLLSIGDIDAGSTVVIAGDLRPSTDRIMRACVQAIVDSGCQVENAGKIPTPALV
                     SHAISTGRAGVMVTGSHIPFDRNGIKINKSVGEVLKSDEPGISREVEGVRVEEYSRTA
                     TTSAFDASGMLKRVTDLPPLDRSGEEAYVRRYLTSFTRGGLSGLGVLVYQHSAVGRDI
                     LPQILRELGAEVVTAGRSDTFVSIDTENITDE"
     gene            complement(828..1841)
                     /gene="moaA"
                     /locus_tag="VFN58_05365"
     CDS             complement(828..1841)
                     /gene="moaA"
                     /locus_tag="VFN58_05365"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET8917452.1"
                     /translation="MLHDIYARPLKDLRISVTDRCNFRCTYCMPFDEYEWIDKKEILT
                     FEEIARLADLFVQLGVDKIRLTGGEPLLRRDLEKLISKLSSLNGLEDLCLTTNGSLLA
                     EKVSALKSAGLRRINVSLDTLNSQKFARIRRRGDLAQVLEGLFAAKRCGLHPIKINVV
                     VERGVNDDDIIDLVEFSRKNGFAIRFIEYMDVGNSNNWTSEKLVAKKEILDIVDARYP
                     LREVGRDKGSAPSMDYEFIDGRGELGMIASVTEPFCSTCTRARLTADGKLVTCLFSAT
                     GYDLKALLRNGATDEEIIEIVSAIWRKRTDRYSDERLEAMNSGRGYRAKDHKKIEMIT
                     LGG"
     gene            complement(1893..>2183)
                     /locus_tag="VFN58_05370"
     CDS             complement(1893..>2183)
                     /locus_tag="VFN58_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET8917453.1"
                     /translation="WILVGGITGWLTGTFMGEQGYGKSLLGGYARSLDIVLGIVGASF
                     GGYLFFWVVIGEGSSFSRYATAILGSVTLVGVVRLISARNLVWVRNNRFISF"
BASE COUNT          512 a          633 c          529 g          509 t
ORIGIN      
        1 tgttcgtcag tgatgttctc cgtatcgatg gaaacgaacg tgtcgctgcg tccggcggtg
       61 accacctcgg cgcccagctc gcgcaggatc tgcggcaaga tatcgcggcc caccgccgaa
      121 tgctgataca caagaacgcc caggccggaa agaccgccac gggtgaaact cgtcagataa
      181 cgacggacgt aggcttcctc gccggagcga tcgaggggcg gcaggtctgt cacccgcttg
      241 agcattccgg aggcgtcgaa ggccgatgtg gtcgcggtcc ggctgtactc ctcgacgcgc
      301 acaccctcga cttcgcggct gatgcctggt tcgtccgact tcaacacctc gccgacgctt
      361 ttattgattt ttataccgtt acgatcgaat ggaatgtggc tgccggtcac catcacaccg
      421 gcgcgtccgg tggagatggc atgcgagact aacgcgggtg tgggaatctt cccggcattc
      481 tccacctggc agcccgagtc gacaatcgct tggacgcagg cgcgcatgat gcggtcggtg
      541 ctcggccgga ggtcgcccgc gataaccact gtactgcccg catcaatgtc gccgatgctc
      601 aacaaatagc ggagcgcagc tttgacattg atataggcct cgaggtcagt gatgtctttg
      661 accaggccgc gcaaaccgct ggtgccaaag gccaacggga ccggcgtgta attaagaaac
      721 gacgccgtag ggcgtacaag cggttcaggc attggagtta aggtgttgta aactcgcctt
      781 gaggccggcg atgatcccga tggcgatcag aagcgatcat cgccggatca tcctcccaag
      841 gtgatcatct ctattttctt atgatccttt gccctataac cgcggcccga attcatcgct
      901 tcgagtcttt cgtcggagta tctatctgtc ctttttcgcc aaatcgcact aacaatctcg
      961 attatttctt cgtcggtggc tccgtttctc aataatgcct tgaggtcata cccggttgca
     1021 gagaacagac aggtcactaa tttcccgtca gccgtgagcc gcgctcgggt gcaagttgag
     1081 caaaacggct cggtgaccga tgcgatcatc cctagctctc cccggccatc gataaactcg
     1141 taatccatag acggcgcgct tcccttgtct ctgcccactt ctctcagggg gtagcgagca
     1201 tcgacgatat cgagaatttc ttttttggcg accagcttct ccgaagtcca gttgttcgag
     1261 ttgcccacat ccatgtattc gataaagcgg atagcaaatc cgttcttcct ggagaactcc
     1321 accaaatcga tgatgtcatc gtcgttgaca cctcgctcaa ccaccacatt aatcttgata
     1381 ggatggaggc cacaacgctt ggcggcaaaa aggccctcca gcacctgcgc cagatcgccc
     1441 cgccttcgta tccgggcaaa cttctgagag ttaagtgtat cgagacttac gttgatccgc
     1501 cttagtcccg cagatttaag ggcagaaacc ttttccgcga gcagcgagcc attggtcgtt
     1561 aggcacaaat cttctaagcc gtttaaggat gaaagtttgc ttattaattt ttccagatcc
     1621 ctccgcaaga gcggctctcc accggtcagc ctgatcttat ccacccctaa ttggacaaag
     1681 aggtctgcga gtctggcaat ttcctcaaaa gtaaggatct cctttttatc aatccactca
     1741 tactcatcaa aaggcatgca ataggtgcag cggaaattac acctgtcggt aactgatatc
     1801 ctcaggtctt ttagcggacg agcataaata tcgtgcaaca tctaaatccc tcgttcgata
     1861 tcagtacgct ctagagcgaa tatgcaactc tttcaaaacg aaatgaatct attgtttctc
     1921 acccaaacta agtttcttgc ggaaatcagc cgaaccactc ccacgagagt cacagagccg
     1981 aggatcgcgg tggcatatct gctaaaagag ctgccttccc cgatgactac ccagaaaaat
     2041 agataacctc caaaggaggc accaacgata cccaacacga tatctaagct tctggcataa
     2101 ccacctagta agcttttccc gtacccctgt tcccccataa atgttccggt caaccaaccg
     2161 gttattccgc ccactaaaat cca
//
LOCUS       DASSWZ010000222         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_206066, whole genome shotgun sequence.
ACCESSION   DASSWZ010000222 DASSWZ010000000
VERSION     DASSWZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206066"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /gene="topA"
                     /locus_tag="VFN58_05375"
     CDS             complement(<1..2157)
                     /gene="topA"
                     /locus_tag="VFN58_05375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET8917454.1"
                     /translation="MAAKNLVIVESPAKAKTLERYLGRDFQVKASVGHIVDLPKSKLG
                     VDIKKNFTPDFHVIQSKKKVIEDLKKAAKGKENIYLASDPDREGEAIAWHIADQVAKN
                     HKRVHRVLINEITRRAVQEAIAKPLELDRNKFDAQVARRILDRLVGYQISPLLWNKVR
                     RGLSAGRVQSVAVRLVCEREKEIRAFEPLEYWSLTALLEGRLPPSFKARLVQWQGKKI
                     DNKKFRLENEPSVQDIVKSLDGASWVIGEVEKKERRRYPTPPFVTSKLQQEAARKLGF
                     QPRRTMQLAQQLYEGIELGSEGAVGLITYMRTDSTRVSNEALTAVRHFIEGQYGKSYV
                     PDKPNTYRSKKGAQDAHEAIRPTSMEYSPERVRRYLRRDVFQLYSLIWDRFVASQMVP
                     ALFDQTAFEIPVREAVFRATGQQIKFDGFMKIYIEGRDEPAPQANGADQDGDEDESRE
                     NDGVLPDLQKGEALKLLSMDPRQHFTQPPPRYTQASLIKELDEKGIGRPSTYATIISN
                     ILDREYVVQDDRRSLAATELGFLVTDLLVESFPDILNVEFTAGMENELDKIEEGKEAW
                     TKAMKRFYTPFSRDLKKAAKEMRDVKRQEVPTDIACEKCGAMMVVKWGRNGEFLACPR
                     YPECKNTKNFKRNDKGELHVAKEEEVNETCEQCGRPMQLRWGKYGKFLGCSGYPECTN
                     IRPLEKPVDLGIKCPECKEGNLIERKSRRGKVFYGCD"
     gene            complement(2203..3366)
                     /gene="dprA"
                     /locus_tag="VFN58_05380"
     CDS             complement(2203..3366)
                     /gene="dprA"
                     /locus_tag="VFN58_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET8917455.1"
                     /translation="MDVIDATLSFGREYVLPKVAERNLARWIALSRVQGLGCVSFKKL
                     AGHFEDPTEALSAPTATLAEIPGLDQNVIDGLRNFSAWDEAEKEIIRAEKAAVKIVPF
                     TDATYPARLRMIPDPPPLLYLKGEIRSEDQKAVAVIGSRSTSDYGRRVARDLCRGLAS
                     LGFTVVSGLARGIDGTAHETSLNAGGRTIAVLGSGVDRVYPAEHDKLYQRIIENGAVI
                     SEFSIGTRPLAFNFPARNRLISGLSLGVVVVEATEKSGSLITAALALEQGREVFAVPG
                     EVGASRSRGAHRLIRQGAKLVETVDDIIEEIAPQLVVRGGKAAIASRRTLPQSLGVEF
                     QKIFALFEERPVQIDEVIERSGHSPSRVSEILLELELQGYIRQLPGKKYTAER"
     gene            complement(3728..4711)
                     /locus_tag="VFN58_05385"
     CDS             complement(3728..4711)
                     /locus_tag="VFN58_05385"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007671114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET8917456.1"
                     /translation="MAMSLLEIRDLKKHFPVGEGLFSRNKGAVKAVDGVSLSVEEGET
                     LGIVGESGCGKSTLGRTILRLIEPTSGEVYFQGKNLLALSQRELRDARRQMQIIFQDP
                     YASLNPRMRVGDIVGEGLEIHKLARGRAKRDRVIELLRQVGLREDHYNRYPHEFSGGQ
                     RQRIGIARALAVTPKFIVCDEPVSSLDVSIQAQIIYLLQELQEKMHLTYFFISHDLRV
                     VEHISHRVAIMYLGKIVEIAKSDTIYRDAKHPYTRALLSAVPIADTSRKKERTVLEGD
                     VPSPVHPPSGCSFHPRCPHREAVCDQKEPPLEFSTDGHGVACHVFGPAKQS"
     gene            complement(4719..>5691)
                     /locus_tag="VFN58_05390"
     CDS             complement(4719..>5691)
                     /locus_tag="VFN58_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8917457.1"
                     /translation="NLQTSFFTPEGEVRAIDGVSFDVAEGKTLGLVGESGCGKSVTSL
                     SIMRLIPSPPGKIVGGEILYRGHDLLKLSNEEMRKIRGNEISMIFQEPMTSLNPVFTV
                     GNQIGEAIRLHQGLGKKQTREKTIEMLRLVKIADPESRVDSYPHQLSGGMRQRVMIAM
                     ALSCNPSLLIADEPTTALDVTIQAQILELMKELQQKIGMALLLITHDLGVVAEQADEV
                     AIMYAGKIVEHTSAQAIFARPFHPYTVGLLNSLPGTGDKKKKRLDAIPGVVPSPLHLP
                     SGCRFRDRCPKAAGLCADAEPQLVEKQQNHFVACHFPETGLRAGAQN"
BASE COUNT         1194 a         1694 c         1469 g         1334 t
ORIGIN      
        1 gtcgcatccg taaaagactt ttccccgccg cgacttgcgc tctatcagat tgccttcttt
       61 acattccgga catttgatgc cgaggtccac cggcttctcg agcggccgga tgttagtaca
      121 ttcggggtaa ccactgcagc cgagaaactt accgtatttc ccccagcgca gctgcatcgg
      181 ccggccgcac tgctcgcaag tctcgttgac ttcttcttcc ttggcgacgt ggagttcgcc
      241 tttgtcgttg cgtttgaaat tcttggtgtt cttgcattcc gggtagcgcg ggcaggccaa
      301 gaactcgccg ttacggcccc acttcaccac catcatggcg ccgcactttt cacaggcgat
      361 atccgtcggc acttcctggc gtttgacgtc acgcatttct ttcgcggcct ttttgagatc
      421 ccgcgagaag ggggtataga accgcttcat cgctttcgtc cacgcctctt tcccctcttc
      481 gattttgtcc agttcattct ccatcccggc ggtgaactcc acgttcagaa tatccgggaa
      541 actctccacc aagagatcgg tgaccagaaa tccgagctcc gtcgccgcca gcgaccggcg
      601 gtcgtcctgg acaacgtatt cgcgatccag aatgttggaa atgatcgtcg cgtaggtcga
      661 cggacggccg atgccctttt catcaagttc cttgatcaat gaagcctggg tgtagcgcgg
      721 cggcggttgg gtaaagtgct gccgcggatc catggagagt agcttcaatg cttctccttt
      781 ctgcaggtcc ggcaacacgc cgtcattctc gcgactctcg tcctcgtccc catcctggtc
      841 tgcgccgttg gcttgcggcg cgggttcatc ccgtccctcg atatagatct tcatgaaacc
      901 gtcgaacttg atctgctgcc cggtggcgcg aaagacggct tctcggacag ggatctcgaa
      961 cgccgtctga tcgaacaagg ctggcaccat ttgactggcg acgaagcgat cccagatcag
     1021 agaataaagt tggaacacat cgcgccgcaa atagcgccgc acccgttccg gtgaatattc
     1081 catcgacgtc ggacgaatcg cctcgtgcgc gtcctgcgcc cctttcttag atcgataggt
     1141 gttcggctta tcgggaacat agctcttgcc gtattgccct tcgataaaat ggcgcacggc
     1201 ggtgagtgct tcgttagaca cccgtgtgga gtcagtgcgc atgtaggtga tcaggccgac
     1261 ggcaccttcg ctgcctaact caatgccttc atagagctgc tgggcgagct gcatcgtgcg
     1321 cctaggctga aaccccagct tgcgcgccgc ttcctgttgc agcttgctgg tgacaaatgg
     1381 cggggttgga tagcggcgcc gctctttttt ctccacctcg ccgataaccc aggaagcccc
     1441 gtccaaggac ttgacgatat cctggacgct cggctcgttc tcaagccgaa acttcttgtt
     1501 gtcgattttt ttcccttgcc actgaaccag acgggccttg aatgacggtg gcaggcgccc
     1561 ctctaacaat gcggtgagcg accagtattc caggggctca aaggcgcgaa tttctttttc
     1621 gcgctcacag accaaacgca ccgcaaccga ttggactcgc ccggcgctca agccacggcg
     1681 aactttgttc cagaggagcg ggctaatctg gtaacccacc aagcgatcca aaatgcgccg
     1741 cgcgacctgg gcatcgaatt tgtttcgatc tagctcaagg ggcttggcga tggcctcttg
     1801 aaccgcccgg cgtgtaatct cgttgattaa tacgcggtga acccgtttat ggtttttggc
     1861 gacctggtca gcgatatgcc aggcgatggc ttcgccttcg cggtccgggt cggaggcgag
     1921 atatatgttc tctttccctt tggccgcctt ttttagatct tcgatgactt ttttctttga
     1981 ttggataacg tgaaaatccg gcgtaaagtt cttcttaata tcgacgccta atttgctctt
     2041 gggcaggtca acgatatgac cgactgaggc cttcacttga aaatctcttc ccaaatatct
     2101 ctccaaggtt ttcgccttgg ctggtgattc cacgatgact agattcttcg ccgccatagc
     2161 acttcaccca tttcttaaac ttaacacgcg tagcttgaga ttctaccgct ccgcggtgta
     2221 cttttttccg gggagctgtc tgatatatcc ttgcaattcc aattccaaca agatttcgga
     2281 aaccctcgac ggggaatgac cgctcctctc aatgacttca tcgatttgga ccggtcgctc
     2341 ctcaaataag gcgaaaatct tttgaaactc aactcccagg ctttgcggaa gcgtacgccg
     2401 cgacgcgatc gccgctttgc cgccacgcac caccagctgc ggtgcgattt cttcgataat
     2461 atcgtccacg gtctctacca gtttcgcccc ctgacggatc aaccggtgcg cgccgcggct
     2521 gcgactcgcg ccgacttcac ccgggacggc aaaaacttcc cgcccctgct ccaaagccaa
     2581 ggccgccgta atcaaagagc cgctcttctc cgttgcttcc accactacga cacccagcga
     2641 taaaccgctg atcagtcgat tgcgggccgg aaaattgaac gccagcggcc gtgtgccgat
     2701 cgaaaattcg gaaataacgg cgccgttttc aatgatccgc tggtagagct tgtcatgctc
     2761 cgcgggatag acgcggtcga cgcccgaacc caaaactgcg atggttcgac ctccggcatt
     2821 caacgaagtt tcatgtgccg tgccgtcgat accgcgcgcc agcccgctca cgacggtaaa
     2881 gcccaatgat gccagacccc gacacagatc tcgcgccacc ctgcgtccgt aatcgctcgt
     2941 gcttctcgag ccaatcacgg caacagcctt ttgatcctcg ctgcggatct ctcccttcag
     3001 ataaagcaaa ggcggtggat ccggaatcat ccgcaatcgc gccggataag tcgcatctgt
     3061 gaacggcact attttgacag ctgccttttc agccctgatg atctcctttt cggcctcatc
     3121 ccatgccgaa aaattgcgta gcccgtcgat aacattctga tccaaccccg gaatttcagc
     3181 caatgttgca gtcggtgccg acaacgcctc tgtgggatct tcgaagtgtc cagctagttt
     3241 cttgaagctg acacacccga gtccctgcac ccttgacaaa gcgatccaac gagcgagatt
     3301 cctttcagct actttcggca aaacatactc tcttccgaat gatagtgtcg cgtcaattac
     3361 gtccatttaa actcggccat ttcgggctga accgtgagga ttctcagaat cgagacacaa
     3421 gctgcaaatg ataaggcaaa atcggcgcga acgctagcac ccagtatcga gcgatctgca
     3481 atcccaagag gcatcgtgcg ttatctgtca acgcacagtc cacgccccag gcactccagc
     3541 tcaaacaaaa atcttagccc atgagaaaag caaactccag gcatgatgga aaaaactttc
     3601 atatctcatc tgcatcgcca gaataagact tatcccgcag ttggaggatt tctaattagt
     3661 tcgacactac gaaccgactg gcgctcgtca agtcgtggac agatagctta ctgagtaata
     3721 gaatttttca gctttgtttt gccgggccga acacgtgaca ggcaacgcca tgaccgtctg
     3781 tgctgaactc cagcggcggt tccttctgat cgcagacggc ttcgcgatgc ggacagcggg
     3841 gatgaaaact gcaacctgaa gggggatgga ccgggcttgg gacgtcgcct tcaagaacgg
     3901 tgcgctcttt tttacggctg gtatcagcga tgggaaccgc ggacagcaaa gctcgcgtgt
     3961 agggatgctt tgcgtcccga tagatcgtat cgctcttggc aatctcgacg atcttgccga
     4021 ggtacatgat cgccactcga tggctgatgt gctcgaccac ccttaggtca tgggagatga
     4081 aaaagtaggt caggtgcatc ttttcctgca gctcctgcag gagatagatg atctgcgcct
     4141 gaatcgaaac atccagcgag gagaccggct cgtcgcagac aataaatttg ggggtcacgg
     4201 ccaaagcgcg agcgataccg atgcgctggc gctgcccgcc gctaaactcg tgcgggtaac
     4261 ggttgtaatg atcttcacgg aggcccacct gccggagcaa ttcgatcacc cggtcgcgct
     4321 tggctctgcc cctggcgagt ttgtgaattt ccaagccttc tccgacgata tcgccgacgc
     4381 gcatccgcgg gttcagcgaa gcgtagggat cctgaaaaat gatctgcatt tgccgacgcg
     4441 catcgcggag ttcgcgttgg gacagagcca gcaggttttt tccctggaaa taaacttcgc
     4501 cgctggtagg ctcgatgagc cgaagaatgg ttcgtcccaa tgtcgacttg ccgcagccgg
     4561 attcgccgac gatgcccaag gtttcgcctt cctccacgct caaactcaca ccgtccaccg
     4621 ccttgactgc acccttattg cgcgagaata gcccctcgcc caccgggaaa tgcttcttga
     4681 ggtcacgaat ttctaacagg ctcatagcga ttcgcacatc aattttgcgc gcccgcccgc
     4741 agcccggtct cggggaaatg gcaggcgaca aaatgatttt gttgtttttc taccaactga
     4801 ggctccgcat cagcgcacag cccggcggct ttggggcagc ggtcccggaa tcgacagccg
     4861 cttggaagat gcaacgggct cgggacgacc cccgggatgg cgtccaagcg ctttttcttt
     4921 ttgtctcccg tgccgggcag agaattgagc agacccaccg tgtagggatg aaagggtcgc
     4981 gcgaagatgg cttgcgcgct cgtgtgttca acgatcttac ccgcatacat gatcgcgacc
     5041 tcgtcggcct gttccgccac gacaccgaga tcgtgggtaa tcagtagcag agccatgccg
     5101 attttttgct gcagctcttt catcagctct agaatctgcg cctgaatcgt tacatccaat
     5161 gccgtcgtcg gctcgtcggc gatcaggagg ctcggattgc aggacagcgc catggcgatc
     5221 atcacccgct gccgcatgcc accgctcaac tgatggggat aagaatccac ccgagactcc
     5281 ggatcggcga tcttgaccag ccgaagcatt tcaatggttt tttcgcgtgt ttgttttttc
     5341 cccagccctt gatgcaggcg gatcgcttcg ccgatctgat tacccacggt aaaaaccgga
     5401 ttcaacgagg tcatgggctc ttgaaaaatc atggagatct cgttgccgcg gatcttgcgc
     5461 atctcctcgt tgcttagctt gagcagatca tggccgcgat agagaatctc cccgccgacg
     5521 atctttcccg gcggcgacgg aatcagacgc ataatcgaaa gcgacgtcac gctcttgcca
     5581 caaccggact cgccgacgag cccaagggtt ttcccttccg ctacatcaaa actgacgccg
     5641 tcaattgccc gtacttcgcc ttcgggcgta aaaaaggagg tttgaagatt c
//
LOCUS       DASSWZ010000223         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_36488, whole genome shotgun sequence.
ACCESSION   DASSWZ010000223 DASSWZ010000000
VERSION     DASSWZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36488"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFN58_05395"
     CDS             complement(<1..185)
                     /locus_tag="VFN58_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917458.1"
                     /translation="MSYETGLSMEERVTSLFQPDTLLPEQYLDTFRRKLHLEPEKKLM
                     LAVLEDAIACFQKYVFAR"
     gene            complement(249..707)
                     /locus_tag="VFN58_05400"
     CDS             complement(249..707)
                     /locus_tag="VFN58_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET8917459.1"
                     /translation="MGLMQIPRRVDYGLRAVIYLSVQDPERCCSITEIATQQGVPKKF
                     LEKIIQDLIHGGLIKSKRGACGGYTLARSPEEISFYEVIEALEGPIAVNACMDQHVSC
                     EQLPRCSMVNVWSEVQRKVTEVFTNTTIADLKKKPCRDLIGGSASLSSAA"
     gene            complement(712..1926)
                     /gene="nifS"
                     /locus_tag="VFN58_05405"
     CDS             complement(712..1926)
                     /gene="nifS"
                     /locus_tag="VFN58_05405"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HET8917460.1"
                     /translation="MGSEIYFDNNATTRVLPEVAAVMQPYLTELYGNPSSIHRFGAQV
                     AQKIGEARAQVAGLIGAADPIEVIFTSCGTEGDNAAIRGMVEARPDKRHIVTTQVEHP
                     AVLGLCQHLEKRGYRVTWLSVKQDGMLDLEELRNALTDDTALVSIMHANNETGVIFPI
                     DKIGAIVKARGIPFHVDAVQAAGKIPLKVKDSPVDLLTISGHKFHAPKGIGALYVRRG
                     VAFPPFIIGGHQEKNRRAGTENVAGIVGVGKAAELAQENLVEQSKRIRVLRDQLEEEL
                     LRSCPDCRVNGQASERLPSTSNVSFRHLEGESILVLLDQFGICASTGSACTAGSSEPS
                     HVLRAMGVAPDWLQGAVRFSLSRFNTAEEVHYVNEKVPLIVQRLQGLSALGKLAGQQE
                     KPRAGNGIGVRG"
     gene            complement(1958..>2665)
                     /locus_tag="VFN58_05410"
     CDS             complement(1958..>2665)
                     /locus_tag="VFN58_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HET8917461.1"
                     /translation="NQLHAAVVELVAHDDAQIKYSTVQNWYPGDAEGKGGIYNFVTKR
                     GKCVGKRSKISWTQVETGSAITWKYPSCILQGDDSVGEFYSVALTNNYQQADTGTKMM
                     HIGKNTKSTIISKGISAGHGQNTYRGLVKIMKGATGARNYSQCDSLLLSDKCGAHTFP
                     YLEVLNNVSQVEHEASTSKIGEDQLFYCRQRGISSEDAVNMIVNGFCKQVFRELPMEF
                     AVEAQKLLGVSLEGSVG"
BASE COUNT          609 a          811 c          614 g          631 t
ORIGIN      
        1 cgcgcaaaaa cgtatttctg aaagcacgca atggcatcct ccaacaccgc cagcatgagc
       61 tttttttccg gctccaaatg caacttcctc cggaaagtgt ctagatattg ctccggcaga
      121 agcgtgtcgg gttgaaacaa cgaggtgact ctttcctcca tgctcaatcc agtctcgtaa
      181 ctcataaaaa ccaccttcct ataaagcgca gcctcccttc caactgcgat aaaggcatcc
      241 tcgaactatc acgcggcgct tgagagcgaa gcggagccgc cgatcaaatc ccggcaaggc
      301 tttttcttca aatccgcgat tgtcgtgttg gtaaacacct cggtaacttt tcgttgaacc
      361 tcactccaaa cgtttaccat tgaacaacgt ggcaattgct cacaactcac atgttggtcc
      421 atgcaggcat tgaccgcgat gggcccctcg agcgcctcga tgacctcgta gaacgaaatc
      481 tcttcgggag aacgagcgag agtatatccg ccgcatgccc cccgcttgga tttgatcaga
      541 ccaccatgta tcagatcttg aataattttc tccaaaaact ttttcggcac tccctgctgt
      601 gtggcaatct cggttatcga gcagcatcgc tccgggtctt ggaccgaaag ataaataaca
      661 gcgcgtaacc catagtcgac ccggcgcgga atctgcataa ggcccatagc gttaccccct
      721 gaccccaatg ccgttgcccg cccttggctt ctcttgctgg cccgccaatt ttcccaatgc
      781 agaaagcccc tgtaaacgtt ggacgatcaa aggcaccttt tcgttcacat aatgaacttc
      841 ttccgccgta ttaaaacggc tcagactaaa ccgaaccgct ccctgcaacc agtccggggc
      901 cacacccatg gccctgagca cgtgggaggg ttccgaagaa cccgccgtgc aagcagaacc
      961 tgtggaggcg caaatcccga attgatccaa aagaaccagg atcgactccc cttccaaatg
     1021 tctaaaactg acgttggaag tgctcggcaa tcgttcgctt gcctgaccat taacccggca
     1081 atcaggacag gatcgaagca attcctcctc cagttgatcg cgcagaacgc gaatccgctt
     1141 ggactgctcc accaaattct cttgtgccaa ttccgccgct ttccctacgc ctacgatgcc
     1201 cgcgacattt tcggttccgg cacgccggtt cttctcctgg tgtccaccga tgatgaaagg
     1261 cggaaacgca acgccgcgac gtacatacaa ggcaccaatc cccttgggcg cgtgaaactt
     1321 gtgacccgaa atagtgagca aatccaccgg gctgtctttg actttgagag gaatctttcc
     1381 cgccgcctga actgcgtcca catgaaacgg aatacccctt gccttgacga tcgcaccgat
     1441 tttgtcgatg ggaaaaatca caccggtttc attattcgca tgcataatcg acaccaatgc
     1501 ggtgtcgtcg gtcagcgcat tgcgcaattc ctccaggtcg agcattccgt cctgttttac
     1561 gctcagccag gtgacgcgat atccccgctt ctcgaggtgc tggcatagcc ccaaaaccgc
     1621 gggatgttct acctgcgtcg ttactatgtg gcgcttatca ggcctcgctt ccaccattcc
     1681 gcggatggca gcgttatccc cttcagtgcc gcaactcgtg aaaataacct cgatcggatc
     1741 ggcggctccg atcaatcccg cgacttgtgc tcgcgcttcg ccgattttct gcgctacttg
     1801 agcaccgaag cgatgaatgc tcgacgggtt gccgtaaagt tccgttaagt acggctgcat
     1861 gaccgccgcg acttccggca aaacccttgt cgttgcgtta ttgtcaaagt agatttccga
     1921 ccccataaga aagtatccca agtcttctat cggctaacta cccaacgctt ccctctagac
     1981 tgacacccag gagtttctgc gcctctaccg cgaactccat cggcaactca cgaaacacct
     2041 gcttgcaaaa accgttgacg atcatgttta cggcatcttc cgaagaaata cctcgctgcc
     2101 tgcagtaaaa cagttggtcc tcgccgatct ttgaggtcga cgcttcgtgc tccacctggg
     2161 agacattatt caacacttcg agatacggga aagtatgcgc accgcatttg tcacttagaa
     2221 gtaaggaatc acattgcgag tagttgcgcg ctccagtggc ccccttcatg attttcacca
     2281 agccgcgata ggtattttgc ccgtgccccg cagagatccc cttggaaatg atggtgctct
     2341 tcgtgttctt gccgatatgc atcatcttgg tgccggtatc cgcctgctgg tagttgttgg
     2401 tcagcgcgac agaatagaat tcgcctacgg aatcgtcgcc ttgcagaatg cagcttggat
     2461 acttccaggt gatcgccgaa ccggtttcca cttgcgtcca ggaaatcttc gaacgtttgc
     2521 cgacgcactt gccgcgcttc gtgacgaaat tatagatacc gcccttccct tccgcatcgc
     2581 ccggatacca gttctgcact gtggaatact tgatctgggc gtcatcgtgg gccaccaact
     2641 ccacgacggc cgcatgcaat tggtt
//
LOCUS       DASSWZ010000224         7066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_192168, whole genome shotgun sequence.
ACCESSION   DASSWZ010000224 DASSWZ010000000
VERSION     DASSWZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192168"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFN58_05415"
     CDS             <1..579
                     /locus_tag="VFN58_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET8917462.1"
                     /translation="DQTLEMHFKLYEGYVKETNVLQEKISAILKDGKVDQEEMPAYSE
                     LKRRLGFEYNGMVLHEYYFGNLKKDGGGDPKRGSAFVKATEESFGGYDIWKTDFVGIG
                     KMRGVGWAICYQDPTNGRLSNHWITLHEIGNVAGYIPVLVMDVWEHAFILDYKPAERP
                     KYIEAFLSNIDWSAVEQRMQAPAQKAQRARAV"
     gene            589..1272
                     /locus_tag="VFN58_05420"
     CDS             589..1272
                     /locus_tag="VFN58_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET8917463.1"
                     /translation="MARKKKILSAAVSVLFLAFAGGCAVQTTSAPPRSPSQEARTPNV
                     KPLDPSQAARLQRIMVPLLRAGDRTRSLKEIRLGVLEDKNINAANAGGGEFYVTTGLL
                     QKANDEQLRGILAHEIAHDDLGHVAKAQVLGTGLNLGVILLEQLIPGSSTITPIAGTL
                     VARAYSRSEELEADQHAVEILQRAGYSKDVMIDALVWVMQSSPADGGGFLSTHPATQD
                     RIASLRQLK"
     gene            1577..1942
                     /locus_tag="VFN58_05425"
     CDS             1577..1942
                     /locus_tag="VFN58_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917464.1"
                     /translation="MQKNILIVDDEENLLLLLEKILTRQGYQVVTAHNTYDARALLET
                     RIFQLAILDIKMFPLDGVFLLGEIKSRSPSTEVIMITAYPTIDNRNECMKKGASTYLT
                     KPVDIQELKTTVDGLLLSH"
     gene            2156..6448
                     /locus_tag="VFN58_05430"
     CDS             2156..6448
                     /locus_tag="VFN58_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET8917465.1"
                     /translation="MTQRSAKNRRAKKPSLPTQDWLVGGGEMGERIRAMNWAETQLGP
                     MESWPQSLRSAVSILLPSKAQIVLFWGPDLIALYNDAYSPVLGIKHSWALGRPARECW
                     SEIWDDLLGPLFKNVIATGESFWAKDHQFLLERHGYIEETYFDVSYDPIRDESGQVGG
                     IFCIVSETTGRVLSERRLGMLRELGADLTAINTEDELFSTIRRRLDAHAKDLPFALVY
                     LCDAEGKQARLRCAHGATAGEAIAPVVIALDSENGMWPASQLLATAAPVTVDDLPARF
                     AAIPTGPWDKPARQAVIFPIAQQGQDRPAGFLIAGINPYRPFDAAYSGFVNLLAGQVA
                     AALAGARAYEEERKRAAVLAELDRAKTAFFSNVSHEFRTPLTLMLGPLDEVLAKPEGQ
                     VLPENREILTIARRNGQRLLKLVNTLLDFSRIEAGRIQAVYEPVDLAAYTSELASVFH
                     SAVEKAGMRLVIDCPPFSEPVFVDRDMWEKIVLNLVSNAFKYTLEGEISVSLRTDNRS
                     AVLSVRDTGTGIPETELPNLFNRFHRVEDARGRTQEGTGIGLALVFELAKLHGGAVGV
                     ESVYGKGSTFTVTIPLGKGHLPPERIGIRRTLESTALGANPFLEEALRWLPDALPKQG
                     EEFFVSDEESHALRYFPTGDGMTKIVLADDNADMRDYMRRLLGANYQVIAVADGQQAL
                     QAIIQDKPDLVLTDVMMPNLDGFGLLKVLRENPETASIPVIMLSARAGEESRVEGLEA
                     GADDYLTKPFSAREMLARIGGALALAKVRGEAAGVLRESEKRLRQLTSLMPAAVYTCD
                     EEGRITFFNRRAVELWGREPKLNDSEERYCGSFRVLRPDGSQIPHSEGIMATAVTTGK
                     PARNEETMIERPDGSRIILRVDIDPLYEINGRVCGAISVFEDVTDLKQAEQASQRLAA
                     IVESSEDAIVSKDLNGLITSWNQAAERVFGYTAEEAIGKPVTLLIPPERHDEEPGILE
                     RIRRGERIENYETVRRHKDGGRLDISLTVSPIRDAKGNIIGASKIARDITRRKRVEVA
                     LRESEQRLGLATQTGKLGVWDWDIVTNRISWSDSLYVIHDVRPDQFDGTMEGFAALVH
                     PEDQEQVSAAIQRALDSDVPYELEFRAVRPDGGVMWLFTSAAVLRDGGRPVRMLGATM
                     DITQRKHTEDALRQSEEKLRQQAQELEQQLIMSGRLVSLGEVTASMAHEFNNPLGIIM
                     GFVEDMLSGMDRADPNYRALQIIDEESKRCRQIVKDLMEYARPRSTELCSTSMVDAIE
                     KTLQLMDNRLYKQKVAVEKKLGADLPRIQADSQQLEQVLVNLYLNAIDAMPEGGKLIV
                     EARIAQSDGLAPLALITVGDTGFGIADTDLPKIFQPFFTAKKRRGMGLGLPICQRIVK
                     NHGGRIEVESEPGKGTTFKIYLPLEQRASTESPEPASTSEISPPVSTARTLS"
     gene            6675..>7066
                     /locus_tag="VFN58_05435"
     CDS             6675..>7066
                     /locus_tag="VFN58_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HET8917466.1"
                     /translation="MGSHETASLLTENDLYLFNEGSHFRLYDKLGAHVVNHEGISGTQ
                     FAVWAPNAAQVFVMGDFNGWDKHSNPLRPNGQSGIWEGFFPGIGNGTLYKYHIVSRFN
                     DYRVDKTDPFSIFNEIPPKTASIVWNLD"
BASE COUNT         1674 a         1857 c         2001 g         1534 t
ORIGIN      
        1 gaccaaactt tggaaatgca ttttaagctc tatgaaggct atgtcaaaga gactaacgtt
       61 cttcaggaaa aaatctctgc catcttaaag gatggcaagg tcgatcagga agagatgccc
      121 gcctattccg aattaaagcg gcgcctgggt ttcgaatata acggtatggt gctgcatgaa
      181 tactatttcg gcaacctcaa aaaagatggc ggcggcgatc ctaagcgagg ctctgccttc
      241 gttaaagcga cagaggagag ctttggtggt tatgacattt ggaagaccga ctttgtcggc
      301 atcggcaaga tgcgcggtgt cggctgggcg atctgctacc aggatcccac taacggccgt
      361 ttatccaacc attggatcac cttgcatgag atcggcaatg ttgccgggta catcccagtg
      421 ttagtgatgg acgtgtggga acacgcgttt atcttagact acaaaccagc cgaacggcca
      481 aaatacatag aagcattcct ttccaatatc gattggagtg ctgtcgaaca acgtatgcaa
      541 gcaccagcgc agaaggcgca acgtgcccga gcagtttgac tagagacaat ggcacgaaag
      601 aaaaagattc tatcggccgc cgtgtcggtc ttgtttcttg cgtttgcggg cggctgcgcg
      661 gtgcaaacta cgtccgcgcc gccgcgctcg ccgtcacagg aagctcgcac gccgaacgtc
      721 aaaccgctcg atcccagtca ggcggcaagg cttcagcgga tcatggtacc gttgttgcgc
      781 gcaggtgata gaactcggtc gctaaaagaa atacgacttg gtgtgctaga ggataagaat
      841 attaacgccg ccaatgcggg tggcggcgag ttttacgtca caacggggct gcttcagaag
      901 gcaaatgatg agcaactgag aggaatattg gctcacgaaa tagcccatga cgatcttgga
      961 catgtcgcta aagcccaagt gctaggaact ggattgaatc tgggtgttat tttactcgag
     1021 caactcattc ccggcagcag caccatcacc ccgatcgcgg gcacactagt cgcacgcgct
     1081 tacagccgat cagaagagtt ggaagcagat cagcatgccg tagagatttt gcaacgtgcc
     1141 ggctactcta aagatgtgat gatcgacgcc cttgtctggg ttatgcaatc ctctccagca
     1201 gacggtggcg gattcttgtc aactcatccg gcaacccaag atcgaatcgc atccttacgc
     1261 caattaaaat gatatcagcg tttctctaaa aattgctcga cttgtaggtt ttttgcttag
     1321 aacgagtgcg cgttttacgg acaaggcttg ttcccgtttc atcgccaacc acgtcgatac
     1381 gccagcttta tggcctagta agaggacgat ctactgttgg aaaatctcct tttttacctg
     1441 gcatttagat tgctcttctc attggtaaat taaccgctgc cagacagtaa agcaccaatg
     1501 gccgttggaa gacctttggt cgcgatatgt gaactaactt agaagtatag aatcaacagc
     1561 aaaagggcag gatgtcttgc agaaaaacat actgatcgtt gacgatgaag agaatctttt
     1621 attgctcctc gaaaagattc ttaccagaca gggttaccag gttgtaacag cgcacaacac
     1681 ctacgacgca cgcgccctcc tggaaacgcg cattttccag ctggctatcc tcgatatcaa
     1741 gatgtttccg ttagatgggg tattcctttt gggcgaaatc aaaagccgat ctccttctac
     1801 agaagtgata atgatcacgg cctatccgac catcgacaat cgcaacgaat gcatgaaaaa
     1861 aggggcttcc acttatctga cgaagcccgt ggatattcaa gaactgaaga ccactgtcga
     1921 tggcttgctg ttgtcccatt gagaatccgc tggcgaatca agaatcttgt tgcgttctcg
     1981 ctgaccgacc ccccgctttg ttatttctcc atttaaggta tgctctagga atgatagagc
     2041 aatgctagaa agctgtaggc acaaaggtgg agaaatctga tggcatcatg gcgccgatgt
     2101 tgctggcggg tacgggggat agatcacctg tcggaccttt agaaacaccc aaaaaatgac
     2161 ccagcggagc gctaagaatc ggcgggcaaa gaaaccgtct ttaccaaccc aagattggtt
     2221 ggttggcggc ggcgagatgg gcgagcggat tcgcgcgatg aattgggccg agacgcagct
     2281 cggaccgatg gagtcgtggc cccagagcct gcgaagcgcg gtgagcattt tgctgccatc
     2341 aaaggcccag atcgtccttt tttgggggcc ggatctgatt gcgctctaca acgatgcata
     2401 cagtcctgtc ttaggcataa agcattcctg ggcgctcggc agaccggcgc gggaatgttg
     2461 gagtgagatc tgggatgact tactcgggcc gctattcaag aatgtcatag caacgggcga
     2521 atcattttgg gcgaaagatc atcaattcct cctcgaacgg cacggctaca tcgaagagac
     2581 gtatttcgac gtctcatacg atcccattcg agatgagagt ggccaggtcg gcggcatctt
     2641 ctgcatcgtc agcgaaacga ctggacgtgt gctgagcgaa cgacggctgg ggatgctgcg
     2701 cgaattggga gccgacctca cagcgatcaa taccgaagat gagctgttct ccacgattcg
     2761 ccgccgtctg gatgctcatg caaaagatct cccttttgca ttggtctatc tatgcgatgc
     2821 cgaaggaaag caggcgcggc taaggtgcgc tcatggcgca actgccggcg aagctattgc
     2881 gcccgtagtc atcgctctcg actcggaaaa tgggatgtgg ccggcgtcac agcttctcgc
     2941 gaccgctgca cccgtgactg tggacgatct cccggcgcgc ttcgctgcca tccccaccgg
     3001 gccctgggac aagcccgcta ggcaggcggt catttttcca atcgctcagc aaggtcagga
     3061 tcgtcccgcg ggctttttga tcgcgggtat caacccctat cgaccctttg atgcagccta
     3121 cagcggattc gtcaatctgc tggcgggtca ggtcgccgcc gctcttgccg gtgcacgtgc
     3181 ctacgaggaa gagcgcaaac gggcggctgt gctggccgag ctcgaccgcg ccaagacggc
     3241 gtttttttcc aacgtgagcc atgagtttcg cacgccgctc acgctcatgc tcgggcccct
     3301 cgatgaagtt ctggcaaagc ctgaggggca ggttttacct gaaaaccggg agattctaac
     3361 catcgcgcgc cgcaacggcc aacggctgtt gaagctggtg aacactctgc tcgatttttc
     3421 ccgcatcgag gcgggccgga tccaagcggt gtatgagccg gtggatttgg ccgcctacac
     3481 ttcggagctg gcaagcgtat ttcattcagc ggttgaaaag gctggaatgc ggctcgtgat
     3541 tgactgcccg cctttttcgg agccggtctt cgtggaccgg gatatgtggg agaagatagt
     3601 tctcaatctt gtttccaacg ctttcaaata caccctcgag ggcgagatat ccgtatcact
     3661 gcgtaccgac aaccgctcgg ccgtcttgtc cgtccgggac accggcacgg ggataccgga
     3721 gacggagctg ccgaacctct tcaaccggtt tcaccgggtt gaggatgcca ggggacgcac
     3781 acaagagggc accggcatcg gcctggcgct cgtctttgag ctagccaaac ttcatggcgg
     3841 agccgtcggg gttgaaagcg tttacggcaa gggcagcact tttacggtga ccatccccct
     3901 aggcaaagga caccttccac ccgaacgcat cggcatacgc cgaacactcg agtccacagc
     3961 tcttggagct aatccgttct tggaggaggc gctccgatgg ctgccggatg cattgccaaa
     4021 acagggcgaa gaattttttg tatctgacga agagtcgcac gctttgcggt attttccaac
     4081 tggcgacgga atgaccaaga ttgttttggc cgacgacaat gccgacatga gggattacat
     4141 gcggcgactt cttggcgcga attaccaagt cattgccgtc gccgatggac agcaggcact
     4201 ccaggccatt atccaagaca agccagactt ggtgcttacg gatgtgatga tgccgaatct
     4261 ggacggtttc ggtttgttga aggttctacg ggaaaatccg gagacggcct cgatcccggt
     4321 gatcatgctt tctgctcgcg ccggagagga gtcgcgcgtc gaaggcttgg aagccggcgc
     4381 tgatgactac ctgactaagc cgtttagcgc tcgtgaaatg ctggcccgga tcggcggcgc
     4441 tctggctctt gctaaggtgc gcggcgaggc cgccggcgtg ctgcgcgaaa gtgaaaaacg
     4501 cttgcgccag ctcacctccc ttatgccggc agccgtctac acttgcgatg aagaagggcg
     4561 aatcacattc tttaaccgtc gcgcggtgga gctctgggga cgcgaaccga aactgaacga
     4621 tagtgaagaa aggtactgtg gatcatttcg cgtgttgcgg ccggacggtt cgcagattcc
     4681 tcacagcgag ggaattatgg caacagccgt gacaacgggg aagcccgctc gaaatgaaga
     4741 gactatgatc gaacggcccg acggctcccg gatcattctc agagtagaca tcgacccgtt
     4801 gtatgagatc aacggccgcg tctgtggcgc gatcagcgtc tttgaagacg tcacggattt
     4861 aaagcaggcc gaacaggcca gccagcggct ggccgccatc gtcgagtctt ccgaagacgc
     4921 aatcgttagt aaagacctta acggcctcat taccagctgg aaccaagccg cggagcgggt
     4981 ctttggctac acggctgaag aggcgatcgg caaacccgta acccttctca ttccgccgga
     5041 gcgccacgat gaggagccgg gtatccttga gcgcatccgg cgcggcgaac gcatcgaaaa
     5101 ctatgagacg gttcggcggc acaaggacgg cggtcgactg gacatttccc tgaccgtgtc
     5161 gcccatcaga gatgccaaag gaaacatcat cggcgcgtcc aagattgccc gagacattac
     5221 ccggcgcaag cgcgtagaag tcgcgctgcg tgagagtgaa cagcggctgg gtctggctac
     5281 gcagaccggt aagcttggcg tctgggattg ggacatcgtc accaatcgga tctcatggag
     5341 tgattcgctg tacgttattc atgatgtgag gccggatcag ttcgacggga ccatggaagg
     5401 tttcgccgcc ctggttcatc ctgaggatca ggagcaagta tcagcggcga ttcagcgcgc
     5461 gctggacagc gatgtcccct acgagctgga gttccgcgcg gtccgccccg acggcggggt
     5521 tatgtggctt tttaccagcg ccgcggtcct gcgcgatggc ggccggccgg ttcggatgct
     5581 tggcgccacc atggatatca cccagcgcaa gcacacggag gatgcgctgc gccagagtga
     5641 agagaagcta cgccagcagg ctcaggaact ggagcaacaa ctgatcatgt cggggcgatt
     5701 ggtttccctc ggcgaggtga cggcttccat ggcccacgag ttcaataatc ccctcggcat
     5761 catcatgggg ttcgtggaag acatgttgag cggcatggac cgggccgatc ccaactatcg
     5821 cgctttgcaa atcatcgacg aggagtcgaa acggtgccgg cagatcgtaa aagaccttat
     5881 ggaatacgcg cgccccagaa gtacagagct ttgctcgacc tcgatggtcg atgccattga
     5941 gaaaacgctt cagttgatgg acaaccgtct ttacaaacag aaagtcgccg tggagaaaaa
     6001 actcggggcg gacctgccgc gaatccaggc cgattcccag cagctggagc aggttctagt
     6061 gaatctctat ctaaacgcga ttgacgccat gccggaaggc ggcaagttga ttgtggaagc
     6121 gagaatagcg caatctgatg gcctggcgcc gctggcgttg ataacggtgg gagataccgg
     6181 gtttggcatc gcggatacgg atctgccaaa gatttttcaa ccgtttttca ccgccaagaa
     6241 gagaagaggg atgggtttag gactccccat ctgtcaacgg atcgtcaaga atcacggcgg
     6301 gcggatcgaa gtcgaaagcg agccgggcaa agggacaacc tttaagattt atttgcctct
     6361 cgagcagagg gcgtcaacgg agtcgcctga gccggcaagc acatctgaaa tttcaccacc
     6421 agtttcgacg gcaagaacgc tgtcctaaca accctattgt agcttctatc agggaatctt
     6481 ggctctcagg ttcaagttcc ccgatagaca aacccatatt catctgtcag aatttttgta
     6541 taagctgcgt ttcgaagcaa tcgaacaatg cgcaattttt cgctttaaga gttttatcaa
     6601 agaacttttc cggcggatat ttaaactcgc tccgccacac ccggaaaact aaagaaagaa
     6661 ggtaatgaag atcgatgggg agccatgaaa ccgcaagcct cctgaccgag aatgatctat
     6721 acctctttaa cgagggtagc cactttcgac tctacgacaa actgggcgct catgtcgtca
     6781 accacgaagg tatcagcggc actcagttcg ccgtttgggc gcccaatgcg gcgcaggttt
     6841 ttgtcatggg cgatttcaac ggctgggata agcacagcaa ccctctccgc cccaacgggc
     6901 agtcgggcat ctgggaaggg tttttccccg ggatcggtaa cgggacactt tacaagtatc
     6961 acattgtttc tcgctttaac gactatcgag tagacaaaac ggatccgttt tcgattttca
     7021 atgagatacc gccaaagacg gcgtccatcg tgtggaatct cgatta
//
LOCUS       DASSWZ010000225         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1267859, whole genome shotgun sequence.
ACCESSION   DASSWZ010000225 DASSWZ010000000
VERSION     DASSWZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267859"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1980)
                     /locus_tag="VFN58_05440"
     CDS             complement(148..1980)
                     /locus_tag="VFN58_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET8917467.1"
                     /translation="MDTPTINDLNICVATENGSGSASSNNILFKAIFKMGIPCSSKNM
                     FPSNIQGLPTWYQIRASAEGYLSRKDVIDVMVMFNDATASKDIYRVRDGGLILYDDST
                     PLPAHLKRDGVQYLGVPANKLIAKLVPASPLRAKQRNMIYVGALAYLFGIDMEVIKAV
                     LEDTFGKKPAVIESNLLCIDAGYQYVKEKGFTQSIARLQVIPNGNKGKILTEGNTAAA
                     LGAIYGGASVICWYPITPSSSLAEAMEYYLPKLRKPKDGKRTYAIVQAEDEIASAGMV
                     AGAGWAGARAMTSTSGPGVSLMNETIGLAYYAEIPCVFFIIQRGGPSTGLPTRTQQAD
                     ISLMYGASHGDTRHILLIPHDMNSCFDFARRSFDFADRFQTPVFVAMDLDLGMNFWSS
                     DPLSLEKEPYDYGKRLSAEQLEEWTKAGKKFRRYFDQDGDGIPYRTVPGNPHRMASYF
                     TRGSGHDADARYSEDENDYKYILDRLKKKFETARKFVPKPIVEKERGVKTGIICYGSS
                     YEATREARDRLKARGLKTNHMLIRALPLTKEVRDFLVEHDTIYFVEQNRDAQMAAIIK
                     DENPELGAKIVSILVYNGLPITPKAIVDQIFDASQSAELKTAQM"
     gene            complement(2046..2879)
                     /locus_tag="VFN58_05445"
     CDS             complement(2046..2879)
                     /locus_tag="VFN58_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET8917468.1"
                     /translation="MTTNLTTASVASSIQEVLEMMVTADVGRVIVTNKEVPVGIFTEK
                     DVMRRVINNNRDPRKSTIKEVMTSPIRAVREETHIVEAFGKMYRGKYRHLLVRGKRGK
                     IVGIVSMRRILGLAVELGQGMSETKTLGSLVSGGVLSVDESSSVYETIDLMIQRGVAS
                     IVVSVAGKPRGIFTERDVLKRVAAKGIDTRKTQVKEVMTSPLVTMADTALIGEALTEM
                     YKRDIRNMPVVAAGGELIGILSMPDILQYAAAFNIDERVRQNWKEVQEYYDSEDQYTP
                     G"
     gene            3101..3415
                     /gene="clpS"
                     /locus_tag="VFN58_05450"
     CDS             3101..3415
                     /gene="clpS"
                     /locus_tag="VFN58_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET8917469.1"
                     /translation="MSRRETEFDRELVTETKKKLKKPPLYKVLLHNDDYTTKEFVVQV
                     LQYVFHKEHTEAVQIMLHVHKKGIGVAGVYTYEVAETKVTLVEGLARQHEYPLKCTME
                     EA"
     gene            3417..>4481
                     /locus_tag="VFN58_05455"
     CDS             3417..>4481
                     /locus_tag="VFN58_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET8917470.1"
                     /translation="MISKELEGTLNTALKEAKSRRHEYVCLEHLLYALLQDKDASGAI
                     INCGGDIERLRKGLEEFFQTHLETLPGGSDHQPQQTLSFHRVLQRAVIHAQSAEKKEI
                     NGGNLLIAMFREPDSYAVYLLQEQGITRFDLVNYVSHGISKIAAAEEWSQSEEEQSEE
                     EKPDRRVKPLEAFAVNLVAKAQQGNIDPLIGRDDEIERTIHVLCRRRKNNPIYVGDPG
                     VGKTALAEGLALKIHHGEVPDVLKNAVIYALDMGALLAGTKFRGDFEARLKGVLAGLK
                     KLPNSILFIDEIHTIVGAGATSGGSMDASNILKPALASGELKCIGSTTYHDYKSYFER
                     DRALARRFQRIEVPEPSQDEA"
BASE COUNT         1067 a         1163 c         1148 g         1103 t
ORIGIN      
        1 tcaatgctgt tttcgaagta cgcgcgcata accgacgctg aaatcgcgtc gtggccacag
       61 cctcggcaca tagtcgaggg cgcgccttcg taatcctttt ccgttagatt aatccgattt
      121 agtttcgctt ctgccatgat tctatcctta catctgtgct gtttttaatt cggccgattg
      181 cgacgcatca aagatctggt ccacgatcgc ttttggcgtt ataggtaagc cgttgtagac
      241 gagtatactg acaatcttcg ctcccaactc gggattttcg tctttgatga ttgctgccat
      301 ctgagcatcg cggttctgct ccacgaagta aatggtgtcg tgctccacca agaaatcccg
      361 tacttccttc gtaagcggta gggcccggat taacatgtgg ttagttttca atccccgcgc
      421 tttcaacctg tcgcgggcct cgcgcgtcgc ctcatagctg gaaccataac aaataattcc
      481 ggttttgacc cccctttctt tctctacgat tggcttgggg acaaatttcc gggcggtctc
      541 aaacttcttt ttcagtcgat cgagaatgta tttatagtcg ttctcatctt cggagtacct
      601 ggcgtcggcg tcgtggcccg atccgcgtgt aaagtaggag gccatgcggt gcggatttcc
      661 ggggaccgtt ctgtaaggta tgccgtcacc gtcctgatca aagtaacggc ggaacttctt
      721 gccggccttg gtccattcct caagttgctc tgcgcttaat cgcttgccgt agtcgtaagg
      781 ttctttttcc aaactgagcg ggtctgagga ccaaaaattc atccccaggt ccaggtccat
      841 agccacgaaa accggggttt gaaatcggtc agcgaaatcg aaactacggc gcgcgaagtc
      901 aaagcaggaa ttcatgtcgt gggggatcaa cagaatatgg cgggtgtcgc cgtgggaagc
      961 accatacatg agagagatgt ccgcttgctg cgtccgtgtc ggcagaccgg tcgaggggcc
     1021 gccgcgctgg atgataaaaa acacgcacgg aatctcggca tagtaagcaa ggccaatggt
     1081 ttcgttcatc aaagagaccc caggcccgga agtcgaagtc attgcacggg ctccggccca
     1141 cccagcaccg gcaaccatgc ctgcagatgc aatctcatct tctgcctgta caatcgcata
     1201 agttcgtttg ccgtctttgg gcttacgcag cttgggcaga taatactcca tggcttcggc
     1261 gagggagctg gatggcgtga tgggatacca acaaatgacc gaggccccgc catagatcgc
     1321 tcccaatgcg gccgctgtgt tgccctccgt aaggattttc cctttgttgc cattagggat
     1381 cacctgcagc cgagcgatac tttgcgtgaa gcctttctcc tttacgtact ggtagccggc
     1441 atcgatgcaa aggaggttgg actcgatcac tgcaggtttc tttccaaagg tgtcctccag
     1501 caccgccttg ataacttcca tatcgatgcc gaataaatag gccagcgcac cgacatagat
     1561 catattacgt tgctttgcgc gtagaggtga ggcaggcacc agtttcgcga tgagcttatt
     1621 ggccggaact cctaggtatt gaaccccatc acgtttcaga tgagctggga gcggagtgga
     1681 gtcgtcatag agaatcaaac caccatcccg cacccgataa atgtctttgg acgcggtggc
     1741 gtcattgaac atgaccatca catcaataac gtctttgcgg cttaggtagc cttccgcgga
     1801 agcccgaatt tggtaccaag tgggcaagcc ctgaatattc gatggaaaca tgttcttcga
     1861 agagcaaggg atgcccattt taaagatcgc cttgaacaat atgttgttcg aggatgcgct
     1921 gcccgaccca ttctcggtgg caacgcagat gtttaaatca ttgattgtag gggtatccaa
     1981 aatgcgacct ccttgagatt ttataactct ccaatcctag catatttctc ttaaaagaca
     2041 acatgttagc ccggtgtata ctgatcttcg gaatcatagt attcctggac ttctttccag
     2101 ttttgccgaa ccctttcgtc gatgttgaag gcagcggcgt attgtaagat atcaggcata
     2161 gaaaggatgc ctataagttc gccccctgct gccacaaccg gcatgtttct gatgtctcgc
     2221 ttgtacatct cagtcaaggc ttcgccgatg agcgctgtat ccgccatggt gactagaggc
     2281 gaggtcatca cttccttcac ctgagtcttt ctcgtatcaa tccccttggc agccacgcgt
     2341 ttgagaacgt cgcgttcggt aaagatacca cgcggttttc cagccaccga aacaacaatc
     2401 gaggcgacgc ctctttgaat catgaggtcg atggtttcat aaaccgatga gctttcgtcc
     2461 acggacaaga ccccgcccga cacgaggctt ccgagtgttt tcgtctcgct cattccttgc
     2521 cccaactcga ccgcaagtcc aagaatccgg cgcatagaaa cgatgccgac gatttttcct
     2581 cttttgccgc gaacgaggag atggcgatat tttcctctgt acattttgcc aaaagcttca
     2641 acgatatggg tttcctcgcg tacagctcgt atcggggagg tcatcacttc tttgatcgtt
     2701 gattttctgg ggtccctgtt attgttaatg acccgcctca tgacgtcttt ttcagtaaaa
     2761 attccaaccg gtacttcctt atttgtgacg ataacgcgtc cgacgtccgc cgttaccatc
     2821 atttctaaga cctcttgaat cgaactagcg accgaggcag tcgttaggtt cgtagtcatt
     2881 atctcgctga ctttgttaga aagcacagga cacctcccta gagatgacta ctcgaagatc
     2941 cacatagttc aggggcgagc ctctaaatca aatataacat ttttttcact tcggggtgca
     3001 agcggttaga aatatttttg ggtgttgacg aggtcgctgc ggaggattgc gaccgggcgg
     3061 agaagcgtgt tagatatata tcgcttccag gagccatatc atgagccgca gggaaaccga
     3121 attcgatcgt gagctggtca ccgaaaccaa gaaaaaactt aaaaaacccc ccctctacaa
     3181 agttttgctg cacaatgacg attatactac caaggagttc gttgtgcagg tgctgcaata
     3241 tgttttccac aaggaacata ccgaagcggt tcaaatcatg ctccatgtac acaaaaaagg
     3301 cattggagta gctggcgttt atacctatga ggtcgcggaa accaaagtga ctttggtcga
     3361 aggtctggct cggcagcacg aatatccact gaaatgcaca atggaagagg cctaaaatga
     3421 tcagcaagga gttggaagga acgctaaata cggctttgaa ggaagcgaag agccggcgcc
     3481 atgaatacgt ttgcttagag catctccttt acgccttgct gcaagacaaa gacgccagcg
     3541 gcgcgatcat taattgcggc ggcgacatcg agcggctccg taagggttta gaggagttct
     3601 ttcaaaccca tctggagacc cttcccggtg gatcggacca ccaacctcag cagaccctca
     3661 gctttcatcg cgtgctgcag cgcgcggtga tccatgccca gtcggcggaa aaaaaggaga
     3721 tcaacggagg taatctattg atcgcaatgt ttcgcgagcc ggattcctac gcggtctacc
     3781 tcttgcagga acaaggtatc acgcgcttcg acctggtgaa ttatgtttcc catgggatct
     3841 ccaagattgc cgcggcagag gaatggtcgc agagcgagga ggagcagagt gaagaagaaa
     3901 aaccggaccg gcgggtcaag cctctcgaag ctttcgcagt taacttggta gcgaaagctc
     3961 agcaaggcaa tatcgacccg ctcatcggac gcgacgacga gatcgaacgg acgattcacg
     4021 ttctctgtcg gcggcgcaag aataatccaa tttacgtcgg cgaccctggc gtaggaaaaa
     4081 cggcgttggc cgaggggcta gcgctgaaaa tccatcacgg cgaagtcccc gacgttctga
     4141 aaaacgctgt gatctatgcc cttgatatgg gcgcgctgct cgccggcacg aagtttcgcg
     4201 gcgatttcga ggcgcgtctt aaaggggttt tagctggtct taaaaaactg cccaactcca
     4261 ttcttttcat cgacgagatt cacaccatag tcggtgccgg cgcaaccagc ggcgggtcga
     4321 tggacgcctc aaatattcta aagcctgccc tcgcgtcggg agaattgaag tgcattggtt
     4381 cgaccacgta tcatgattac aagagctact ttgaaagaga tcgggcctta gcgcgccgtt
     4441 tccagaggat cgaagttccc gaacccagcc aggacgaggc c
//
LOCUS       DASSWZ010000226         7514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_319703, whole genome shotgun sequence.
ACCESSION   DASSWZ010000226 DASSWZ010000000
VERSION     DASSWZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319703"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFN58_05460"
     CDS             <1..220
                     /locus_tag="VFN58_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917471.1"
                     /translation="LSYLKDSFDMLYKEGATHPKMLSIGLHSRVSGRPGRALAVARFI
                     EYAKGFPKVWFAGRDEIAHWWLERCPPS"
     gene            276..1040
                     /locus_tag="VFN58_05465"
     CDS             276..1040
                     /locus_tag="VFN58_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HET8917472.1"
                     /translation="MFLSHLPENPKAKERYAKIQGQLNGYASAGTQVELCFPDDYPGA
                     KLSSDLGARNEVPGIYHVLQTPAIIRKIVWAEKNGYDAVVQSNTFDPGVEAARQAVRI
                     PVIGVFRAALHAASVLSNSIAIIAPLKEHIAYAWQIVHSYGMQGFVKDITAIGLYDET
                     AEKRIDEIFARAAEVMRATIKTSGAGCIIPLGGAVVPTLVSPKDLEREVGVPVLNTKA
                     IGIRFAEMCVQLGMTQSALNYPLAQLRYEDFTVCAR"
     gene            complement(1170..2570)
                     /locus_tag="VFN58_05470"
     CDS             complement(1170..2570)
                     /locus_tag="VFN58_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8917473.1"
                     /translation="MADELCYLSIAAAGRLLKSKQLSPVELTKAFLARIEAIDSQIHS
                     FVLVTPEEALRQAKAAEIEMTSNRYRGPMHGIPIGLKDLIETSGIRTTAHSKVLIDHI
                     PSEDATVVSHLKETGAVLLGKLGCLEFAHGSPSPDQAWPAVRNPWNPEHGFTGGSSTG
                     SAAAVAAGLVMGALGTDTGGSIRNPASFCGIAGLMPTYGRVSRKGVIPYSFSLDHCGP
                     MAWTAEDCAVMLQAIAGYDPHDPGSANVPVPDYTAALNEGISGVRIGVIRHFYEPHLP
                     PEDEMRRAFEAALLELTKVGAVLEEVRLRELEDYDDCKVIISEAEFFAVHEKDLIERL
                     QDYGANLRFRALPGGLIRAVDYIQAQRQRHKLVAEMQTLFAGYDAMVTLCTYGPAPKI
                     STEEPTHFFQRPNLTAVFNVTGNPAISICTGFDRTGLPLAMQIVGRPFDETTTLRIAH
                     AYEQATPWRRLRPSLV"
     gene            complement(2579..2725)
                     /locus_tag="VFN58_05475"
     CDS             complement(2579..2725)
                     /locus_tag="VFN58_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917474.1"
                     /translation="MLKRAGVTVPVEQLARCCAVVARLEAAARRLRKDLKRNDEPASV
                     FIRR"
     gene            complement(2825..4216)
                     /locus_tag="VFN58_05480"
     CDS             complement(2825..4216)
                     /locus_tag="VFN58_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917475.1"
                     /translation="MTTVIHHATIVTGDRDPSIHFNAAIAIEKDLIAAIGPTDEVMAR
                     SPNADVVDGRGKLVMPGLANCHTHFVRILARGIFEDQNAPNRPPFTRKGRVPFPPLSP
                     EQRAAMARLGALEALRSGTTAAMDITNNIEDYAEAIVATGLRLVLAEQVSDRAKGVRV
                     GEPGVFEANPELAGRGLQRISDLHAKWHGAKNGRITVAVAAHAPDMVSPALLQALRDL
                     REKLDSVATIHLNQYWGEVEVIKNSYGMLPTEYLARHGFLHERLVAAHCRCMTPEEEK
                     ILGASGTSVSFNPVVAARSGNSPRITDLEALGCRIALGTDEFTEDMVQVLRGAVLIER
                     LRLKESERPRPEEAMLWGTVNGYRALNLKGCGFLQVGSKADLIVASTRRAHLVPTLRP
                     VAIFVYQGQARDVESVMVDGHWLMRDGKVLSMDEEEVIREADRVSREIWLRYFKDHPD
                     LEMPDGFDTGFPG"
     gene            complement(4253..5311)
                     /locus_tag="VFN58_05485"
     CDS             complement(4253..5311)
                     /locus_tag="VFN58_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917476.1"
                     /translation="MAHREEANNLWRNVMKTLERSFFVILFLALLDTSALPQGASDSK
                     LIEGAKKEKRLVHWTTMTLSQSKQVVDAFQKKYPFIETDLFRTGGDAMLNKIFSEDRA
                     GKHLWDVILTRGDMFLPLMKRNLLASYRSPETKKIPADLFDKNGYWTGYYVNPYTLGY
                     NTKWVKKDEVPKTYEELLNPKWKGQKISVDNTSYNLLAGLITAWGKDKAIAYFRKLAA
                     QEPSVMRGDTNRVQLAAAGEFPLIISFAPIIQRATSEGAPIDWIPLEPVSVQVNPLLL
                     GAKSAHPNAAKLFIDFVLSEEGQKQLVGLSRIPVREDVKPQPARLFGGYKRIVESPEG
                     YENFSEVIKLYQEIFNIR"
     gene            5963..6136
                     /locus_tag="VFN58_05490"
     CDS             5963..6136
                     /locus_tag="VFN58_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917477.1"
                     /translation="MGTYAHYRLLLKLGLVRFDQFSNGFLGRCVVGMEQLEHMRIFSN
                     DFTVGAAPLSRRA"
     gene            6398..6538
                     /locus_tag="VFN58_05495"
     CDS             6398..6538
                     /locus_tag="VFN58_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917478.1"
                     /translation="MTAIRLGLHLHPPPRQRLDAFVDTVKREDRYGFANVQDDALACQ
                     RK"
     gene            6557..7462
                     /locus_tag="VFN58_05500"
     CDS             6557..7462
                     /locus_tag="VFN58_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917479.1"
                     /translation="MSKFIIQPHGRLNDWVADEKGYFTEEGLDYELNVEGSRKNTPRL
                     AAVDSPTPLDDNRFGAFEHYAEGHGRKGEGAGDVSCACHWTVNQAAKVEEGVMWGKAY
                     SVCEAAIVVPGDSNVSEPRDLARTDVAVGYHSGSHYSALQALEVFLAPTDIALKFVGS
                     SWSRVDAALARKTPAVNVWGPQLYLLEQHGFRRIVSTTFMMAFMFPRHVDSGNVERYM
                     RSLVRAQADIDMQPEQFKHYFINEIPERFRDKIDVRRFGVGERVVPQPYTRAMFEKTQ
                     AWMHARNLFDAAPDTGVSYEQAVDI"
BASE COUNT         1685 a         2034 c         2077 g         1718 t
ORIGIN      
        1 cttaagttat ctaaaagata gctttgacat gctctacaaa gaaggcgcga cccaccctaa
       61 gatgttgtca atcggattgc actcccgtgt gtcgggccgt ccagggcgag cgctggctgt
      121 ggcgcgattc atcgaatatg cgaaaggctt tcccaaggtt tggtttgcgg gccgggatga
      181 gatcgcccac tggtggctgg agcgatgccc gccgagttga aagctcacaa gcctcagaag
      241 gcgaaataaa gaaaggggaa tgaaggatgc gaatcatgtt cctcagccat ctgccggaga
      301 atcccaaggc caaagaaaga tatgcaaaga tccaaggcca actcaacggt tacgcatctg
      361 cgggtacgca ggtcgagttg tgcttcccgg acgactaccc cggagccaag ctttcttcag
      421 atctcggcgc gcgaaacgaa gtacccggaa tttatcacgt gctgcaaacg ccggcgatca
      481 ttcgaaagat cgtttgggcc gagaaaaacg gttacgatgc cgtggttcag agcaacacat
      541 ttgaccctgg tgtggaagcg gctcgtcagg cagtgcgcat ccccgtcatc ggcgtatttc
      601 gcgcggcgct tcacgcggcc tcggttcttt ccaactcgat cgcgattatc gcgcccctca
      661 aagagcacat cgcatacgca tggcagattg ttcactccta cggtatgcaa ggctttgtca
      721 aagatattac tgcaatcggc ctctatgacg aaacggcgga gaaacgaata gacgagatat
      781 ttgctcgtgc cgctgaggtc atgcgcgcca ccatcaaaac aagcggcgcg gggtgcatca
      841 ttcctctggg tggcgcagtt gttccgaccc ttgtctctcc taaggactta gaacgagaag
      901 tgggtgtacc agtcctgaac acaaaagcta tcggcattcg atttgccgaa atgtgcgtcc
      961 agctgggaat gacccaaagc gctttgaatt atcccctcgc gcaactccgt tacgaagatt
     1021 tcacggtctg cgcccgatga gtaaaaccca ccgctcggtt actgagacgg tgtccgaaaa
     1081 ccgttcatgg ttcgagagcc tcaccacgaa cggttttgca tgatcaaaac ttcaagtact
     1141 tattcgttcg ttggattgcg acacagtctc tacaccaggg aaggacggag acgacgccag
     1201 ggcgtggcct gctcataggc atgcgcgatg cgaagggttg ttgtttcatc gaaaggcctg
     1261 ccgacgattt gcatggcgag gggcaggccg gtccgatcaa atccagtaca gatggaaatc
     1321 gccgggttgc cggtcacgtt gaagaccgcg gtcaggtttg gccgctgaaa aaaatgggta
     1381 ggttcttccg tgcttatttt cggcgctggc ccgtaggtgc aaagcgtcac catggcatca
     1441 tagcccgcga atagcgtctg catctcagca accagcttgt gacgctgacg ctgcgcttgg
     1501 atataatcaa ctgctcgaat gagcccgccc gggagggcgc gaaagcgcaa gttggcaccg
     1561 taatcttgaa gtctttcgat cagatccttt tcgtggacag caaaaaattc cgcctcggaa
     1621 ataatcacct tacagtcgtc gtagtcctcc agttctcgga gtcttacttc ttcgagcacc
     1681 gcgccgactt tcgtaagttc cagtaaggcc gcttcgaatg cgcgccgcat ttcgtcttcg
     1741 ggaggcaagt gcggttcata gaaatgacgg ataacgccaa tccgcactcc gctgattccc
     1801 tcgttcaacg cggcggtata atcgggtacc ggaacattgg cgctgccggg atcgtggggg
     1861 tcatagccgg caatcgcctg gagcatgacc gcgcagtctt cggcagtcca agccatgggc
     1921 ccgcaatgat cgagagagaa agaatagggg atcacacctt tccggctgac ccgtccatag
     1981 gtcggcatga gcccggcgat gccgcagaaa gacgccgggt tgcggatgga tccgccggtg
     2041 tctgtcccca gtgcacccat caccagtccg gctgccaccg cggcggccga gcccgtgctc
     2101 gaaccgccgg tgaatccatg ctctggattc caggggttcc gtacagccgg ccacgcctgg
     2161 tcaggagagg gactgccatg agcaaactcc aaacaaccga gcttacccaa caaaacggcg
     2221 ccggtctctt tgaggtggct aacgaccgtc gcgtcctcgg aaggaatatg atcgatgaga
     2281 accttcgaat gggccgtagt tcggatgccc gaagtttcaa ttaggtcttt cagaccgatc
     2341 ggtatgccgt gcatcgggcc ccggtaccgg tttgaggtca tttctatctc ggctgccttg
     2401 gcctggcgca gcgcctcttc gggagtaacc aaaacaaaac tatgaatctg agagtcgatt
     2461 gcttcgatgc gagcgaggaa agccttggtc aattccaccg gcgacaattg cttcgatttc
     2521 aagagccgcc cggctgcagc gatagacaag tagcagagtt catcagccat ggcgggatct
     2581 acctccgaat aaaaacactg gccggttcgt cgttgcgttt gagatccttt cgtagccggc
     2641 gcgccgctgc ctcaagtctg gcgaccacgg cgcaacacct tgccaattgc tcaacgggaa
     2701 ccgtgacacc cgctcgtttt aacatcagcg tgacctcctc ctcggatagt tgatgagtgt
     2761 gatcggccat gattcgcctc cctaggggat gtgttgcccg cctttgattg ctaggacgtg
     2821 cggcttatcc gggaaaaccg gtgtcgaaac cgtcaggcat ttccaagtca ggatgatcct
     2881 tgaagtaacg gagccagatt tcccggctca cgcgatccgc ttcacgaatg acctcttcct
     2941 cgtccatcga taacacttta ccatcccgca tgagccagtg cccgtcgacc atcaccgatt
     3001 ccacgtcgcg ggcctgtcct tggtatacga aaatggcaac gggccgtaac gtcgggacca
     3061 gatgagcgcg tctagtgctt gcgacgatca aatccgcctt gcttccgacc tgcaaaaatc
     3121 cgcaaccttt caggttcagg gcgcggtagc cgttgaccgt cccccaaagc atcgcctctt
     3181 cgggtctcgg gcgttcgctc tcttttagac gcaaccgctc gatcagcacc gcgccgcgca
     3241 gcacctgcac catatcttcg gtgaattcgt cggtaccgag cgcgatcctg caacccaagg
     3301 cttccaagtc cgtgatgcgc ggactgttcc ccgaacgcgc cgccaccacc ggattaaaac
     3361 tgaccgaggt ccccgaagca ccgagaattt tttcttcttc cggcgtcatg caccggcagt
     3421 gagctgcaac gagcctctca tggagaaagc catggcgggc caagtattcg gtgggaagca
     3481 ttccgtagct gttcttgatc acttctactt cgccccaata ctgattcaaa tggatggtgg
     3541 cgacggagtc gagtttttct cgcaagtcgc gcagcgcttg caagagcgcg ggagacacca
     3601 tgtccggagc gtgagcggca accgcaacgg tgatgcggcc gtttttcgca ccgtgccact
     3661 tggcgtgaag atcgctaatt cgctgtaagc ctctgccggc gagttcgggg ttggcctcga
     3721 agacgcccgg ctcgccgacg cgaactcctt tggcccggtc cgacacctgc tcggccaaga
     3781 caagacggag tcccgttgca acgatggctt cagcgtaatc ctcgatgttg ttcgtaatat
     3841 ccatggcagc ggtggtgccg ctacgcagag cctcgagcgc gccgaggcgg gccatggccg
     3901 cacgttgctc ggggcttagc ggcggaaacg gaacccgtcc cttacgcgtg aacggagggc
     3961 gattaggggc gttttgatct tcgaagattc cgcgcgcaag gattctaaca aagtgtgtat
     4021 gacagttggc caatccgggc atgaccagct tgcctcgccc atcgacaacg tctgcattcg
     4081 gagaacgcgc catgacctca tcggtcggac caatggctgc gatgagatct ttttcgatgg
     4141 caatcgcggc attgaagtga atggacgggt cgcgatctcc agtcacaatc gtggcgtgat
     4201 gaatgacggt cgtcatgcag ctctcatctc gaaaattgga gcgccttaac aatcagcgga
     4261 tgttaaaaat ctcttgatac agttttatga cctcggagaa attttcatac ccttcggggc
     4321 tttccacgat ccgcttgtat ccaccgaata gtcgggcggg ctgcggtttc acatcttcac
     4381 ggaccggtat ccgactgagg ccaacgagct gtttttgccc ttcttcggaa agcacgaagt
     4441 cgatgaagag tttggccgcg ttggggtgag cagacttcgc tccaagcaac agcggattga
     4501 cttgcactga aaccggctcc agaggaatcc agtcgatggg cgcaccctcg gaagttgccc
     4561 gctgaataat cggggcgaag gaaataatca gcgggaattc tccggccgcg gcgagctgga
     4621 ctctgttggt gtcgccgcgc atgacggatg gctcctgagc ggcgagtttc ctgaagtaag
     4681 caattgcctt gtccttgccc caggcggtga tgaggccggc aagcagatta taggaggtat
     4741 tatcaacgga tatcttttgc cctttccatt tggggttaag caactcctca taagtcttgg
     4801 gcacctcgtc tttcttgacc cacttggtgt tgtaccccaa ggtgtaaggg ttgacgtaat
     4861 agccagtcca atacccgttc ttatcgaaga gatccgcggg aattttcttg gtctcgggcg
     4921 agcgataaga ggccaggaga tttctcttca tcaaaggaag aaacatgtct cctcgtgtaa
     4981 ggatcacatc ccataggtgc ttgccggcgc gatcctcact gaagattttg ttgagcatgg
     5041 cgtcgccgcc ggtgcgaaaa agatccgtct caatgaaagg atatttcttt tgaaaggcat
     5101 ccacgacctg cttgctttgg cttaaggtca tcgtcgtcca atggactaaa cgcttttctt
     5161 tttttgcgcc ttcgatgagc tttgaatctg aggctccctg cggcaaagcg ctagtatcca
     5221 ataaagctaa aaacaggatg acaaaaaagg atcgctccaa tgtcttcatg acgtttctcc
     5281 agaggttatt tgcttcttct ctgtgagcca taacgtcctc ttttgttatt tattccgcag
     5341 taacctgttt tttagtttcc tcattcgttt ctcaattcgc tgaagtgatt gaggaagcgc
     5401 cggaatgtcc tgcaacattt tattaaaacc gatacacgcc attattggca atgaccagat
     5461 agctctacca cccgatttga ccaacaccgc tggattccaa cacagataaa aagccaatga
     5521 cgccgataag tgtgatttga gaccgcccaa aactacgtag cttgtcatgg ctaccctaaa
     5581 tactctccat cgtttccatt cgtcaacagc atttggttat gcagccgcca caggtttaac
     5641 cgtatgccga acctggttca cgtttcagca caggacctgg gttcagcgta gtccctagaa
     5701 ctcttcccga tatgagttat aactcgccag cgttatttca cttcgttggg tggaagcatc
     5761 caagcgacca cgaaaagaac taccacattc gccgaaaaga ttattgatgg gggctttcta
     5821 cggaagggga gcaatcttga tccgcgttgg ggtccgatgc caatggaact aaccgggtca
     5881 gggttgcatg aaagaatcaa gggttcttcg ttgaaacgac atgttgtgca gttgaatcgg
     5941 cgaactaaaa cgacgcgcac gtatgggaac gtatgcgcat taccgcctcc tgcttaaatt
     6001 gggtcttgta cgtttcgacc aattctcgaa tggctttctc ggccgttgcg tcgtcgggat
     6061 ggagcagttg gagcatatga gaatcttctc gaacgatttc accgtcggcg ccgcgccact
     6121 gtcccgacgc gcgtgaagaa atgaggcctt taggaaaacg gggagtgact gtggttttcc
     6181 aggaatttcg cccaatcctc tgaggagacc acgccattgg gtttggcggt gccaaagtat
     6241 agcgagtcgt gaattgctag atggtcgcca tctttgcagc ggacaatata cgttgacgag
     6301 cacgcgctga ctgacagtgt tggaagtcag tcagatcagt tcatgttgac agcaaccgcg
     6361 tcagcgaata cgttcggcgc agatgctgga aaaattgatg actgcgattc gtcttggatt
     6421 gcatctgcat ccgccgcctc ggcagcgtct cgatgcattc gtcgatacgg tcaagcgcga
     6481 ggaccggtac ggatttgcaa acgtgcaaga tgatgcctta gcgtgccaac gcaaataact
     6541 agcggggaga cgcgatatgt cgaaatttat tattcagccg catggccggc tcaacgattg
     6601 ggtcgctgac gaaaagggct atttcactga ggaaggtctc gactacgagc tcaatgtgga
     6661 aggaagccgc aagaacacgc cgcgactcgc cgctgtcgat tcgccgactc cgctcgatga
     6721 caaccgtttt ggagcgttcg aacattatgc cgaaggacac ggacgcaaag gcgagggcgc
     6781 cggcgatgta agctgtgctt gccattggac ggtaaaccag gcggcgaagg ttgaagaagg
     6841 cgttatgtgg gggaaggctt acagcgtttg tgaagcagcg atcgtggttc cgggggattc
     6901 caatgtttct gagccccgcg atctggcgcg cacggacgtc gcggtcggct accattcagg
     6961 gagccattat tccgctctgc aagctctcga ggtatttcta gcacccacag acatcgcgct
     7021 caaattcgtc ggctcgtctt ggtctagggt cgatgcagct ctggcgcgga agacaccggc
     7081 ggtgaatgtc tggggaccgc aattgtacct gctggaacag catggctttc ggagaatcgt
     7141 ctccacgacg ttcatgatgg cctttatgtt tccccgtcac gtggattcag gaaacgtcga
     7201 gcggtatatg cgttccctgg tcagggcaca agccgatatc gatatgcaac cagagcagtt
     7261 caagcactac ttcatcaacg agattcccga gcgctttcgc gataaaatcg acgttcggcg
     7321 tttcggtgtc ggtgaacgtg tggtccccca gccctataca cgagcgatgt ttgaaaagac
     7381 ccaggcctgg atgcacgcac gcaatctgtt cgacgccgcc ccggataccg gggtttccta
     7441 cgaacaggcg gttgatatct gaattgcgct ttattctcta tgcaagcgtg cgagctggtg
     7501 gggtcaggtc ttgc
//
LOCUS       DASSWZ010000227         4614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_871389, whole genome shotgun sequence.
ACCESSION   DASSWZ010000227 DASSWZ010000000
VERSION     DASSWZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871389"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..458)
                     /locus_tag="VFN58_05505"
     CDS             complement(111..458)
                     /locus_tag="VFN58_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917480.1"
                     /translation="MARIRHLAIVTENQERLVKFYTEAFGMKVVEGVGPATYLSDGYI
                     NVAIIKKRPHYKEGLYHFGIEIDDVETLKPLCKELGATSEIQKRPPNREAEYRVADPD
                     GNLIDLSQHGWPT"
     gene            complement(480..1457)
                     /locus_tag="VFN58_05510"
     CDS             complement(480..1457)
                     /locus_tag="VFN58_05510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917481.1"
                     /translation="MKNWRCFCLLAIVLLQPLFLPELRAQGIAISYPGLTGESASLWM
                     ASEGGFFKENGIDAKLVYMEGGRLSIQSLLSGHTQFMAGDAVSAFTAVAGGADVVLLA
                     SAKNVLPYVFAVSKETKRFQDLKGKIIGISQIGGRAGEIARMVIKNGGLDPDRDVTYL
                     AVGGTMSRLATLSGGRVHAAPISRGVMPMAEEKGLKVLEVEPIPLIIDALWTTRKYAE
                     DNPDVIGRVAKAYVAGIAAIVKDKQKALDVLRKYMRTADFKTLDSSYEVYVKGLDKVP
                     VPDDKAIQNTLEITYRVAPKLAGIDIKKHLYFTPVQRLQEEGYIDRLYK"
     gene            complement(1462..1704)
                     /locus_tag="VFN58_05515"
     CDS             complement(1462..1704)
                     /locus_tag="VFN58_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917482.1"
                     /translation="MAKIRAVVVEGDREHGYKRMQVLFGINYFLEITEDGDRVSFLLG
                     SHHEAFKADASEAKGELEKYIKEIMERHPESVFEED"
     gene            complement(1795..2883)
                     /locus_tag="VFN58_05520"
     CDS             complement(1795..2883)
                     /locus_tag="VFN58_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917483.1"
                     /translation="MEIDVMEKIGLRLLLFVGLFIFASTHVSAKEPEEIWRELGKLSE
                     TERQKQLLSGAKTEGKAVLYGNLNAEQLEKLRVDFEKRYALKLEVYRASGERIANRFL
                     TEARGGQFLADVVGPSNEHLPALMKAGLVGRYDSPERAFYPESLRDKQGYWTPYDYNI
                     AVIAYNSRLVPASEVPRKYEDFLDPKWKGNFALDQDPDKSIMGWLKTWGVERTRRYLQ
                     GISKNDVVVRKGHTLLTQLLCAGEFKAGIDLYAYRLADLKNTKGCPVEISYTDPTPAA
                     PSPLVVAKKSPRPYAAALLMDYLLSEPAQKTLAEFGRLSARRGVRPIYPELDTEAKGV
                     RVLLLTPDDAVQLDKPYQQLREEFLLKR"
     gene            3181..3930
                     /locus_tag="VFN58_05525"
     CDS             3181..3930
                     /locus_tag="VFN58_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8917484.1"
                     /translation="MIFRIATLNLEQNHKCWEARRELIVQQLGELRPDMLALNEIHIP
                     EQTGRWLQRGAAERLQMKYTLLQQAKVGDDSRTQAEGLLTRYPVIETASLDYRSHNCV
                     ALAARFEIDGRFLDVYVTHLIAARVEDAARQYQVGQLREWIRTRDDAAFAVVCGDFNA
                     APDQPSIRLMSGAFRPTQIQPTAFTPLQEPAGSPSHPEWERFDRCIDYIWVTESIKVQ
                     VSGLCFNRPAPDNPALWPSDHVGVWADLELS"
     gene            4050..>4614
                     /locus_tag="VFN58_05530"
     CDS             4050..>4614
                     /locus_tag="VFN58_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917485.1"
                     /translation="MKAVRIIALIALIAIPSSAFAWSRGGAVVVRPGFANRAVVVGPG
                     FANRAVFFRPGFPGRVVFVRPGFANRVVFVRPGFPGRFVAPAFVGGVATGIVLNPFFF
                     PRSFYFPGPFINYYPPSSVFSSGYYPPSYAYEVAPPPPSAPAPPDAYDRGYSEGYSQG
                     YEEAQKERDKERYEEGRKRGYEAGYEAG"
BASE COUNT          997 a         1346 c         1146 g         1125 t
ORIGIN      
        1 cgaaagggtc aaaaagttat cggagtagtg catatccggc cggacaggcg ctactgaacg
       61 ctgtattttg ccgcatcgtc gtacgggtca accgagccgg taaaataaac ttacgttggc
      121 caaccgtgct gcgaaagatc gatcagattt ccatcaggat ctgcgactcg gtactccgcc
      181 tctcggttcg gcggtctctt ttgtatctcc gaagttgctc ccaactcttt acagaggggt
      241 tttaaagttt caacatcatc gatctcgatg ccaaagtgat agagaccttc cttatagtga
      301 ggtcttttct tgatgatcgc gacgttgatg tagccgtcgc tgagataggt cgctggcccc
      361 acaccctcta ccaccttcat gccaaaagcc tcggtgtaaa attttaccag cctttcttga
      421 ttctccgtta caatcgctaa atggcgaatg cgtgccatac tcgaactctc ctttcggcgt
      481 tatttgtaca atcgatcgat atatccttct tcctgcaagc gttgaacggg cgtgaagtaa
      541 aggtgcttct tgatatctat cccggcgagt ttcggcgcaa cgcggtaagt gatctcaagc
      601 gtattttgga tggccttgtc gtcagggacc gggactttat cgagaccctt aacgtaaacc
      661 tcgtaggagc tgtccagggt tttgaagtcg gctgtcctca tgtatttgcg caatacatcc
      721 agcgcctttt gtttgtcttt tactatagcg gcgatgccgg cgacataagc cttggcgact
      781 cggccaatca catccggatt atcctccgcg tactttcgcg tcgtccacag ggcatcaatg
      841 attaacggaa tcggttccac ctcaaggacc ttgaggcctt tttcttccgc catcggcatc
      901 acgccccggg agataggtgc cgcatgaacc ctgccgcccg aaagtgtggc cagacgcgac
      961 atggtcccgc ccacggcaag ataggtgaca tctctgtcgg gatccaaacc gccgttcttg
     1021 atcaccatcc gggcgatttc cccggcgcgg ccgccgattt gcgatattcc tatgattttc
     1081 cctttcagat cttgaaagcg tttggtttcc ttggagacgg caaaaacata cggcaagacg
     1141 ttttttgcag aggccaataa aaccacgtcc gctccgccgg caacagccgt aaatgccgat
     1201 acggcatcgc cggccatgaa ttgcgtatgg ccggaaagca ggctttgaat agacagcctg
     1261 cccccttcca tatagacaag ctttgcatcg atcccgttct ctttgaaaaa gcccccttca
     1321 ctcgccatcc aaaggctagc cgattcaccc gtcagacccg gataggagat tgcgatccct
     1381 tgagcccgaa gctccggcaa aaatagtggc tgaagcagaa cgatcgctaa cagacaaaaa
     1441 caccgccaat ttttcatcgt ttcaatcctc ttcaaaaaca ctctccgggt ggcgctccat
     1501 aatttccttg atgtatttct ccaactctcc ctttgcttca gaagcatccg ccttgaatgc
     1561 ctcgtgatgg cttcctagaa gaaagcttac gcgatcaccg tcttcggtta tttccaaaaa
     1621 ataattgatc ccgaataaaa cctgcattct cttgtaacca tgctcgcgat cgccctctac
     1681 gacaacggct cttattttag ccatgcctca cctccctcaa taaaaactgc tcctcgaatg
     1741 gtttgttcac aattttctta cggcctaaag cttcgtcctt tagtcggcga tcacctaccg
     1801 tttcagcaaa aactcctctc gcagctgctg gtagggcttg tccagttgca ccgcgtcgtc
     1861 gggggtcaga agcaacaccc gcacgccttt cgcctctgtg tcgagctctg gatagatcgg
     1921 ccgcacgccg cgacgagccg agagtctgcc gaactccgcg agagtttttt gtgccggctc
     1981 ggagagcaga tagtccatga gcaacgccgc agcgtagggc cggggcgatt tcttcgcgac
     2041 cacgagaggg ctcggcgccg ccggcgtagg atcggtatag gagatttcca ccggacaacc
     2101 cttagtattt ttcaggtccg ccagccgata ggcgtagagg tcgatacccg ccttgaactc
     2161 ccccgcgcag agtagctggg tcagcaaagt gtggcctttg cgcacaacaa cgtcgttctt
     2221 gctgatcccc tgcaaatacc tccgcgtccg ctccactccc caggttttca accagcccat
     2281 gattgactta tcgggatcct gatccagggc aaagtttcct ttccacttcg gatccaaaaa
     2341 atcttcgtat ttccgcggca cctcactcgc cggcaccagc cgggaattgt aagcgatcac
     2401 cgcgatgttg tagtcgtaag gggtccaata accctgcttg tccctgagag attccggata
     2461 gaaagctctc tccggcgaat catagcgtcc caccaaacca gctttcataa gggccggaag
     2521 atgctcattg ctgggcccga cgacatccgc caggaattgg cctcctctgg cttccgtcaa
     2581 gaatcggttg gcgatccttt cacccgatgc ccggtagact tccaacttga gcgcatagcg
     2641 cttttcgaaa tcgaccctga gcttctccag ctgttccgcg ttgaggtttc cgtacagaac
     2701 agccttgccc tcggtcttgg ctcccgacaa aagctgcttt tgtctttccg tctccgacag
     2761 tttgcccagc tcgcgccaga tttcttcggg ttcttttgca gacacatggg ttgacgcgaa
     2821 gataaaaagt cccacaaaga gcaagagtcg caagccgatt ttctccataa cgtcgatctc
     2881 catttcaaaa gatcgtctag cgccgtgacg atgtcgtcgt cgggccttga ttgttttctt
     2941 gtatcataca aacggtcgaa ttccgtcaag agcctcgtgg tcccattcag gaacagagct
     3001 gccggtagtg ggcatagaga ttgtgaaccg gcccgcgccg cccgtagatg aactcagctt
     3061 gcatgcaacg gacaattgga ataaattcct cagaatctat gaagatagga atttttgcat
     3121 tcctattctc tctgctttgc gtacccttta agccctccgc cattaagcga tagactggac
     3181 atgatctttc gaattgcaac cttaaacctc gagcaaaatc ataaatgttg ggaagcccgc
     3241 cgggaactca tcgttcagca actgggcgag ttgagacccg atatgctggc gctcaacgaa
     3301 attcatatac ccgagcaaac cggccgatgg cttcagcgcg gagccgcgga acggctgcaa
     3361 atgaaatata ctctgctgca gcaggccaaa gtcggggacg actcccgcac acaggccgag
     3421 gggctgctca cgcgttaccc ggtcatcgag acggcaagcc tcgactatcg ctcccataac
     3481 tgcgtcgcgc tcgccgcccg ctttgagatc gacggacgat tccttgacgt gtacgtcact
     3541 catctaatcg ccgcccgagt ggaagatgct gcgcggcagt atcaagtggg acaacttcga
     3601 gaatggatcc gaacccgaga cgacgccgcc ttcgccgtcg tctgcggtga cttcaatgcg
     3661 gctcccgacc agccatcgat tcgcctcatg tccggcgcgt tccgtccgac ccaaatccaa
     3721 ccgaccgcct tcacgccgct gcaggaaccc gccggcagcc cgagccatcc tgagtgggag
     3781 cggtttgatc gctgcatcga ctacatctgg gttacagaat cgattaaagt ccaggtttcg
     3841 ggtctttgct ttaataggcc cgcgccggac aatcccgctc tctggccttc agatcacgtg
     3901 ggagtctggg cggatttaga gttgagctga gaggcagatc tcgaccgtgt aaccttcaga
     3961 tcgctctctt gccttcgccc gaagaatgtg gtgaattcca gacaaaggag gtcccggcca
     4021 gagcctgagt tccatcggtg ggaaggccta tgaaagccgt cagaataatt gcgctgatcg
     4081 cactgatcgc tatccctagc tcggcttttg cctggtcacg gggcggagcc gttgtcgtca
     4141 gacccggttt tgccaacaga gccgttgtcg ttggaccggg ttttgccaac agggctgttt
     4201 ttttcagacc cggttttcct ggtagagtag ttttcgttag acctggtttt gctaaccggg
     4261 tcgttttcgt cagaccgggt tttcccggac gctttgtagc gcccgcgttc gttggcggag
     4321 tggctactgg aatagttctg aatccgtttt tcttcccccg atccttttat tttcctgggc
     4381 cttttataaa ttactatccg ccgagtagcg ttttctcttc cggctattat ccaccctcct
     4441 atgcttatga ggtagcacca ccaccgccct ctgccccggc tccgcccgac gcctacgatc
     4501 gaggctactc cgaaggctac tcccagggct acgaggaggc tcagaaagag cgcgataaag
     4561 agcgctacga ggaaggcaga aagcgcggct acgaggcggg ctacgaagcc ggca
//
LOCUS       DASSWZ010000228         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_178152, whole genome shotgun sequence.
ACCESSION   DASSWZ010000228 DASSWZ010000000
VERSION     DASSWZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178152"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFN58_05535"
     CDS             <1..242
                     /locus_tag="VFN58_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917486.1"
                     /translation="PDKVIVVGDTPYDATAAAQADLSAIGLLCGGWTQPQLRGAGCIA
                     VYRDPADLLARYEQSPLHNPTQLHGNSLAPLTAGF"
     gene            327..2264
                     /locus_tag="VFN58_05540"
     CDS             327..2264
                     /locus_tag="VFN58_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8917487.1"
                     /translation="MKKLRTNYYARLDGWSSRKAPAFFFLPTVVLIIASLYWAQAVLI
                     PIAVSILLTFLLTPVADSLERLGLGRVLSVILIVLLAFSFLGAVGWIVTRQLTSVANE
                     LPTYRQNIRKKIADIRGAGKGGALEKVQKTAEEMKQELEKKDGPAQVQPKPREVVVQG
                     EQSSTFWPIPVDFAPILERVASGGLAITLLIFMLIQREDLRNRLIRLIGYGRLTVTTK
                     ALEEASQRISRYLLMQSIINTSFGIAVGIALYLIELPYAPLWGFLAAVLRFIPYVGTW
                     VAVIMPSVLSLIVFQGWMWPALVIAVFLVLELVNNMILEPLLYGESAGVSEVGLLVAV
                     AFWTWLWGPVGLILATPLTVCVVVLSKYVPQLAFISVLMSDEPAMESNISYYQRLLAM
                     DQAEAAEIVEEHLKTHSQEQLFDEVLIPALSHARRDRELGRLTEDGEQFVFRATREII
                     EDLDSLKPRSSPAVTDLSKTATIEKHPSVILPKLRILGCPAHDEADELALLMIRQLLD
                     STRYEVEIIPDAMLTGEVVARIGEKNPAMILVAAVAPGGLAQTRYLCKRVRARFPNLK
                     IAVGRWGTETDDSESMMLAGADKVGTTMVEVHEQIIQLCQIKPDSEVQNGAAAALFSS
                     PKEVIPPEVGQLSKHKASLVK"
     gene            complement(2291..2743)
                     /locus_tag="VFN58_05545"
     CDS             complement(2291..2743)
                     /locus_tag="VFN58_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917488.1"
                     /translation="MDLKPTHGDRVRANTVPEVNRRIDEQIERNIRHYSGQTKEEIHR
                     RIRELDQEWDIERVLETMASSFSLTGIVLGSTVAKRWYLLPTVVLSFLLLHAIRGWCP
                     PLAILRGLGIRTHEEIARERYALKALAGDFAGISKEPSRVEQTLAAVK"
     gene            complement(2934..3101)
                     /locus_tag="VFN58_05550"
     CDS             complement(2934..3101)
                     /locus_tag="VFN58_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917489.1"
                     /translation="MDDGPTEKTTVAETYKSKPLPLELAKLLKKDILCPEMAISVTLN
                     DADKVYLVPMD"
BASE COUNT          795 a          943 c          944 g          870 t
ORIGIN      
        1 ctcccgacaa agtcattgtc gttggggata caccgtatga cgcaacggct gcagctcaag
       61 ccgatttgtc cgcgatcggc ctgctgtgcg gtggctggac gcaaccgcaa ctgcgtggtg
      121 cgggctgtat tgcggtttac cgcgatccgg ctgacctgct ggcgcgctat gaacagtcgc
      181 cgttgcacaa cccaacgcaa ctccacggaa attctctagc acctctaacc gcgggttttt
      241 gacgatcctg ctttctggca taaaaattcc ctttatgcta tccccagtct ggagctgcaa
      301 atcccgctgc cgtgtccgac aaccgcatga aaaagcttcg aaccaattat tatgctcgcc
      361 ttgatggctg gagttcccgc aaggcaccag cttttttctt tctcccgacc gtagtcctga
      421 ttattgcttc gctttattgg gcgcaagccg ttctgattcc gatcgcggtt tcaatcctgc
      481 tgacgttttt gttgacgccg gtggccgaca gtctggaacg actgggtcta ggacgagtcc
      541 tttccgtgat attgatcgtg ctcctggcat tttccttcct cggcgcggtc ggctggattg
      601 ttacacgtca acttaccagt gtggctaacg agctacccac ctacagacag aacatcagaa
      661 aaaaaatcgc cgacatccgt ggggccggta aaggaggggc actcgagaaa gttcaaaaga
      721 cggctgagga gatgaaacag gaactggaaa agaaagacgg gcctgcacag gtgcagccaa
      781 aaccccgtga ggtcgttgtt caaggcgagc agtcatccac gttctggccc atccctgtgg
      841 actttgcacc catcctcgaa cgggttgcta gcggcgggct cgcgatcaca ctgttgatat
      901 tcatgctgat tcagcgtgaa gacctgcgca accgtctgat ccgcctgatc ggctatggcc
      961 gtctaacggt tacaaccaaa gcgctggagg aagcgagcca gcgaatcagt cgctatctcc
     1021 tcatgcagtc gatcatcaat acctctttcg gaatcgctgt cggtatagcg ctttacctca
     1081 tagagctgcc ctatgccccc ctgtggggtt ttctagctgc ggtgttgcga tttatcccct
     1141 atgtcggaac ttgggtggca gtaatcatgc cgagcgtgct gagcctgatc gtattccagg
     1201 gctggatgtg gcccgccctg gtgatcgcag tctttctggt cctcgagctc gtcaacaaca
     1261 tgattctgga gcccttgctc tacggcgaaa gcgcgggtgt ttcggaagtc gggctgctag
     1321 tcgcggtggc gttctggact tggctatggg gacccgtggg actgatccta gccacgccac
     1381 tcacggtctg cgtcgttgta ttgagcaaat atgtcccgca gttggctttc attagcgtgc
     1441 tgatgagcga cgagccggcg atggaatcga acataagcta ttatcagagg ctgctggcga
     1501 tggaccaggc cgaagcggcg gaaatagtgg aagagcatct caagactcac tcgcaggagc
     1561 agctcttcga tgaggtgttg attccggcat tgagtcacgc tcggagagat cgggagcttg
     1621 gccggctgac ggaagatggc gaacaatttg ttttccgagc gacacgggag atcatcgagg
     1681 atctagacag tttgaagccc cgaagttcgc cggcagtaac cgacctttca aaaactgcaa
     1741 cgatcgaaaa acacccatcg gttatccttc cgaaactacg catcttggga tgtccggccc
     1801 atgacgaagc ggacgaattg gcactcctga tgatccggca actgcttgat tccaccagat
     1861 atgaagttga aattattccc gacgcgatgc tcaccggcga agttgttgcc cggattgggg
     1921 agaagaatcc ggctatgatt cttgtcgcag ccgtagcgcc cggcggactg gcgcagacac
     1981 gttacctctg caagcgggtg cgtgcccggt ttcccaatct aaagattgca gtcggccgat
     2041 ggggcacaga aaccgacgat agcgagagta tgatgctcgc gggtgctgat aaggtcggga
     2101 ctacaatggt tgaggtacac gagcagataa ttcaattgtg ccaaattaag cctgattcag
     2161 aagtacagaa cggcgcggcg gcagcccttt tctcctctcc caaggaggtg attccccccg
     2221 aggttggaca actatccaag cacaaagctt ccctggtgaa gtaaacccta aatcttttga
     2281 gccggatgcg ctatttcacg gctgctaaag tttgttcaac tctgcttggc tctttggaga
     2341 ttcctgcgaa gtcacccgcg agagctttaa gcgcataacg ctcgcgggcg atctcttcat
     2401 gagtgcgaat acctaacccg cgcaatatcg ccagcggcgg acaccacccc cggatggcat
     2461 gcagaagcag gaatgaaagc acaaccgtcg gaagtaagta ccaccgttta gctactgtcg
     2521 aaccaagcac aattccggta agcgagaagg aagatgccat tgtttcgaga acgcgttcga
     2581 tatcccattc ctgatctagt tcgcgaatac ggcggtggat ttcttccttg gtttgtccgg
     2641 aataatgacg aatatttcgt tcgatctgct cgtcaattcg tcggtttact tccggtacag
     2701 tattggctct aactcggtcg ccatgtgtag gtttcaggtc catgtttcac ctcctccttt
     2761 agtctcgcac atagagagcc ccatacaata ggatatccgg tttttcgtct agactttgca
     2821 gatatcctta ttcaaatgct ggtcaataga cgtacgcccg agcctaccga tcctttgaat
     2881 gaacaagagg ggaaacctca aaactctgta taatgccctg gcgcctagac cttttagtcc
     2941 atcgggacca agtagacctt atcggcatcg ttcagcgtca ccgatatcgc catttccgga
     3001 cataggatgt ctttcttaag caatttggct agctccaacg gtaagggctt actcttgtaa
     3061 gtctcggcga ccgttgtctt ctcggtaggt ccatcatcca ataaaagagt agctctcgct
     3121 ggatgcgttc gaccgcaacc ggtgcagaaa acaaggatgc gatattgggc cattgcgata
     3181 ccgtgcaaag gttacaactg aagttttaca gcgagggtga cgcctgtcat agcctccctt
     3241 ttcatccagg gaaccgagcg agtctgatcg ctttttcgat ctgcggcaca gcgtcgggac
     3301 aaattgcttt ggcacctccg aaacgtttcc gcctcgtcta gatcacacaa aatagcgatc
     3361 gtgcctgctt gagtctctac ggcgtttttg agccttaagt gcgaacgcaa ggcatcgctg
     3421 gcctgatgca tgagagattt atattgttcg gtggtcagct caatcagcgg ttcatgttgt
     3481 tcgagtaatc gagcctcgct tatggtgtcg gtttgatacc aggtttgcct atgccccgca
     3541 aacgctcatt gt
//
LOCUS       DASSWZ010000229         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_871467, whole genome shotgun sequence.
ACCESSION   DASSWZ010000229 DASSWZ010000000
VERSION     DASSWZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871467"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFN58_05555"
     CDS             <1..603
                     /locus_tag="VFN58_05555"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917490.1"
                     /translation="KYLRFDSGNAAVLDVAIHDEVSGSGQAFCVIKMKKPTSGDVWRA
                     LNASAGYVGSYAKICIAVDEDIDIWDPAMINWAICFNVRPEKDVTVAPGKSPGLDPSA
                     YPPGVPTHVSRMASSHALLIDATRPWAYPPVSLPRKEFMERAKEIWEELGFPELKPRM
                     PWYGYSLGAWTPEDEAEADVALRGDHFVTGEKAKDRRVKP"
     gene            complement(839..1009)
                     /locus_tag="VFN58_05560"
     CDS             complement(839..1009)
                     /locus_tag="VFN58_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917491.1"
                     /translation="MEKGEYVLEVDGKPLVGVVPPWQVEKLSQRREEILALLEQSWEQ
                     NRTVGDAELNKL"
     gene            1556..2122
                     /locus_tag="VFN58_05565"
     CDS             1556..2122
                     /locus_tag="VFN58_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917492.1"
                     /translation="MASPGFTFFKHPVNVEIPAQPPTPNDLFQVVLQTVRFMGVAVAL
                     PDFGTHYPSPDQLKVVVDELIGNHFFSQVVGGIGSAERRFQLVESHSAVDGSFGASCV
                     RFDAKVEELGAYLAPADVVINLNFLSNLVCAHPQPTSTKSSLVWIGFVEVYGEGDQSA
                     AATLSGEVEPFLRSLEFTGPRIVRHQLN"
     gene            complement(2182..3000)
                     /locus_tag="VFN58_05570"
     CDS             complement(2182..3000)
                     /locus_tag="VFN58_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HET8917493.1"
                     /translation="MVKAPPEGAGFDTTLTDGLRIAIRNVIGSASAPDERPLDMSGLA
                     RSLWPDGFHDHWRFVQGPENHDIVYNGREQRVARLGDPSNPRSWYGRSRARVATGISL
                     TAPGIPMLFMGQEFLEDKQWSDNFKFHQNLLLHWAGLDAGDKQMLDHVRFTRELIKLR
                     WRYPGLRGQGFRVVHVHNQNRVLAFHRWVEGEGHDVIVVMHLGNSNRFDYQIGFPGGG
                     DWREVFNSDVYENWVNANTVGNGGRIHAGPQPLHDFSHSAALALPANSLLVFAR"
     gene            complement(2994..4082)
                     /locus_tag="VFN58_05575"
     CDS             complement(2994..4082)
                     /locus_tag="VFN58_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917494.1"
                     /translation="MSASLEHITPNTPMGANLVADGATFRAWAPHARSMHVIGDFNNR
                     QRNDASLLTRDQHGHWRGFIPGVRDRQRYMFYVVGDGGEGPKRDPYARELQTPFPSDC
                     IIRKTDFPWHESGYVTPRFPDFVIYQLHVGTFFTPNLPGKAGTFLDVARKIPHFADLG
                     VTAIQLMPIQEFQTEFSLGYNGTDYFSPEMDFAVENADLGPYLTEVNKLLGAMGQPRY
                     RVEDLRGEMNQLKTLVDLCHLYGLGVILDVVYNHAGGDFGDQSLYFFDRQPSQGGNRN
                     SLYFTDVGHAGGLVFDFGKPEVRDFLIQNAKFFLDEYRIDGLRYDQVSVIDHDGAPHG
                     WSFCQDLTSTLRHHKPTRPNTGTSIPMW"
     gene            4326..>4594
                     /locus_tag="VFN58_05580"
     CDS             4326..>4594
                     /locus_tag="VFN58_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET8917495.1"
                     /translation="MAQIFHRSTNTISRVSVFGGIGIIAVLVTTLAAINRSSYVTDVG
                     VARTQPVQFSHKHHVSDDGIDCRYCHTSVEESSFAGIPSTKICMN"
BASE COUNT         1013 a         1236 c         1334 g         1011 t
ORIGIN      
        1 aaatatctcc gcttcgacag cggcaacgcg gcagtgctcg atgtcgctat ccatgacgaa
       61 gtgagcggca gcggccaggc cttttgcgtg atcaagatga aaaaaccaac ctcgggagac
      121 gtctggcgcg cgttaaacgc cagcgcgggc tacgttggca gctatgcaaa gatctgtatt
      181 gccgtcgacg aagacatcga catctgggat ccggcgatga tcaactgggc gatttgcttc
      241 aacgtcagac ccgaaaaaga cgtcaccgtc gcaccgggca aatccccggg actcgatcct
      301 tccgcctatc cgccgggagt gccgacgcac gtgagtcgca tggcatcgag tcatgcgctc
      361 ttgatcgatg cgacgcggcc gtgggcctat cctccggtgt cgctgccccg aaaagaattc
      421 atggaacggg cgaaagaaat ctgggaggaa ctcggatttc cagagctcaa accgcgcatg
      481 ccttggtacg gttacagtct cggcgcctgg actccggaag acgaagcaga agccgacgtc
      541 gccctgcgcg gcgaccactt cgtgacgggg gagaaagcga aggatcgccg cgtcaaacct
      601 tagtaggatg gagtagtgga atggtggagt attggagtga tggaatcttg cagacagagc
      661 ttttacaacc tgcggacccc gaacaggagc gatgatagat taaaatgatt gtaagacgga
      721 aagaaaggca gccggaggaa caatcaaagt tccttcgtgg cttttgatgt tcaagaggtc
      781 atcatcgccc gtcacgaggt agtcattttt tcccgccgta cctcctcgat tgcttccttc
      841 acagcttgtt taactctgcg tctccgaccg tgcgattctg ttcccagctc tgctcaagaa
      901 gagcgagaat ctcttctcgc ctttgggaga gcttttcaac ctgccaaggg ggaacaaccc
      961 ctaccagcgg tttgccgtcc acctcgagaa cgtattctcc tttttccacg tgtgccctga
     1021 atcgcttagg caggtggcag atctggcgaa cggtgagttg cgttcctcgg agagtatttc
     1081 tctgcggccc tcctggcttt tgctttccta cagtttggaa tgtgccaggc catacactcc
     1141 cattgacttg cgggtgcgag gccaattggc aaccatgtat acagggctgt atccaaagtt
     1201 tcagtactaa caacgagcca atcgaacacc cgcagccaat tctttcgtat tttcgcaaaa
     1261 atcccgactt tcttatttta gtgacgagaa tctgagcctg gcatccagat tgcgttaagc
     1321 tctataggag aacgagttca actatgggag ctgtgacatg agaagatatg tttgcgggat
     1381 agtctgtggg ctgtgtcttg tgctggcggg gttttgtagc gcggccgatt ccatatcaat
     1441 cccgttggat tgcaaaggat tgcaagcttc gggagctgga caggaacgaa tacaagtgcc
     1501 ggattcaggg ttttccgtac ttcccccaca aggcgagaat tggtgtgtga gatcaatggc
     1561 ttcgcccggg ttcactttct tcaaacaccc ggtgaacgta gaaattcctg cacagcctcc
     1621 gacgcctaac gatttgttcc aagtggtgct tcaaacagtt cgattcatgg gggtcgccgt
     1681 agcgcttccg gactttggaa cccactaccc ctcgcccgac cagttgaaag tcgtagtcga
     1741 tgaactgatc ggcaatcact ttttctcgca agtagtagga ggaatcggtt ctgccgagcg
     1801 acgtttccaa ctggtggagt cacattccgc ggtagacgga tcttttggcg caagttgtgt
     1861 ccgtttcgac gcgaaagtgg aagagctagg cgcttacctg gcaccggccg atgttgtcat
     1921 caatctgaac tttctcagca atttggtatg tgcccaccca caacccacgt ctaccaagag
     1981 cagtttggtc tggatcggtt tcgtagaggt atatggtgag ggagatcaat ccgccgcggc
     2041 tacgctgagt ggagaggttg agccctttct acggagtcta gaattcaccg gcccgagaat
     2101 cgttcgccat caactcaact agaaatttgg gctccaacac cgttcacgcc aacgtacgag
     2161 cccaggcttt agtgatgtgc gttaccgcgc gaacaccagc aggctgttcg ccggcagcgc
     2221 gagggctgcg gagtggctga agtcgtgcag cggttggggc cctgcgtgga tccttccgcc
     2281 gttgccgacg gtgttggcgt tgacccagtt ctcgtagaca tcgctgttga aaacctcgcg
     2341 ccaatcaccg ccgccgggaa agccgatttg atagtcgaag cgattggagt tcccgagatg
     2401 catgaccacg atcacgtcgt ggccctctcc ctccacccag cggtgaaacg cgagcacgcg
     2461 attctgattg tgaacatgca caacgcggaa accttgaccg cgcaagcccg gatagcgcca
     2521 gcgaagttta atgagctccc gcgtgaaacg cacatgatcg agcatttgct tgtcgcctgc
     2581 gtcgagtccg gcccagtgca gcagcaggtt ctggtgaaac ttgaagttat ccgaccactg
     2641 tttatcctcg agaaactcct ggcccatgaa cagcatcggg atgccgggcg cggttaggct
     2701 gatgccagtg gccacccggg cgcggctccg gccgtaccag gagcgggggt tgctcggatc
     2761 gccaagccgg gcaacacgtt gctctcggcc attatagacg atgtcgtggt tctccggtcc
     2821 ctggacaaag cgccagtgat cgtggaagcc gtcgggccag aggcttcgcg ccagaccgga
     2881 catatcgagg ggccgctcgt ccggcgcgct ggcactcccg atgacgttgc gaatcgcgat
     2941 gcggagcccg tcggttaacg tggtatcgaa ccccgcgcct tcgggcggcg ctttcaccac
     3001 atagggattg acgttccagt attcggcctt gtcggtttgt gatggcgtaa cgtagaagtg
     3061 agatcctggc aaaagctcca gccatggggg gcgccgtcgt ggtcgatgac gcttacctgg
     3121 tcataacgga ggccgtcgat gcgatactca tcgagaaaaa acttcgcgtt ctgaatcaga
     3181 aagtcgcgaa cctcgggctt gccgaagtcg aacacgaggc cgccggcatg gccgacatcg
     3241 gtgaaataga gggaattgcg attgccgcct tggctcggct ggcggtcgaa aaaataaagg
     3301 ctctggtcgc cgaagtcccc gcccgcgtga ttgtacacca cgtccaggat aaccccgaga
     3361 ccatagaggt ggcagagatc caccagcgtc ttgagctggt tcatctcgcc gcgcaagtct
     3421 tcgacccgat agcggggttg tcccatggcg ccgagcagtt tgttgacctc cgtgaggtag
     3481 ggcccgaggt cggcattttc cacggcaaaa tccatttccg gcgagaaata atcggtgccg
     3541 ttgtaaccta aactgaattc ggtttggaac tcctggatcg gcatgagttg gatcgcggtc
     3601 acgccgagat ccgcgaagtg cggaattttg cgcgcgacgt cgaggaaggt gccggccttc
     3661 ccgggcaggt tgggagtgaa aaaggtcccg acgtgcaact gatagatcac gaagtcggga
     3721 aacctcggcg tgacataccc gctctcgtgc caagggaaat cggtcttgcg gatgatgcag
     3781 tcgcttggaa aaggagtttg taactcgcgg gcgtaaggat cgcgcttggg accttcgccg
     3841 ccgtcgccga cgacatagaa catatagcgc tggcgatcac gcacgccggg aatgaaacca
     3901 cgccagtggc catgttgatc ccgggtcagc agactggcgt cgttacgctg gcgattgttg
     3961 aagtcaccga tcacatgcat ggaccgcgca tgaggcgccc aagcgcggaa cgttgcgccg
     4021 tcggcgacta gattggcgcc catcggggtg ttcggggtga tgtgttcgag ggaagcggac
     4081 acggaatcct ctttaagcga atgctatcta agcttaggtc acttgcaaaa tggcagcagt
     4141 aacactcgtg tttgagagta tttcctcatc cattaaatca cggcgatcga attgcgcaag
     4201 gtaccagggg ggaaatttga tcgctgcggc caaattttac cgggttactt ttcggacgaa
     4261 tatccttgaa acgcgctgat gatacttgct tagctgaaat gacaggctaa agtaagagta
     4321 ggtcgatggc tcagatattt catcggagca cgaacaccat ctccagggtg agcgtattcg
     4381 ggggaatcgg catcatcgca gtcctcgtaa cgacgctcgc ggcaatcaac cgttcctcct
     4441 acgtaacgga cgtcggtgtc gcacgcaccc aaccggtgca attcagtcat aagcatcacg
     4501 tgagtgatga cggcatcgat tgccgctatt gccacacttc ggtggaagag tcctctttcg
     4561 ccggcattcc atcaacgaag atctgcatga attg
//
LOCUS       DASSWZ010000230         6293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_248955, whole genome shotgun sequence.
ACCESSION   DASSWZ010000230 DASSWZ010000000
VERSION     DASSWZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248955"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFN58_05585"
     CDS             complement(<1..214)
                     /locus_tag="VFN58_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET8917496.1"
                     /translation="MLDALFGVFSNDLAIDLGTANTLIYVKNQGIVCNEPSVVAVQQR
                     NERGGKKVLAVGAEAKRMLGRTPGSIV"
     gene            406..2316
                     /locus_tag="VFN58_05590"
     CDS             406..2316
                     /locus_tag="VFN58_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET8917497.1"
                     /translation="MLDVLRKRKRSWIIIFLLGLIIVVFIAFYGGNKFRDPSSLDIAE
                     VNGEVITQREFALHYQKELERYREMFKGSLTPELEKNLNLRGSLLEQLIEKRLVLQEA
                     RHLGLAATDEDLMTAIAQVPDFQVNGRFNKDRYLQLLRASRINPGQFEDEQREQLTIQ
                     RLYSMLVHAVHVTEAEVRDRYRFEQGKINLYFIRLPTASYLSEVKPTEEEIKKFYDRN
                     KELLKEPLKVQVEYLSYPFDRFSSSAQITDKEVEDYYQAHRESRFHKPKEVKARYILV
                     RVPPGADAKQKNDAQTRANRILTEARAGKDFAQLAKKESEDSTAAKGGEVGWLTQAQL
                     PSPLDKAVFNLAKGEISNVIESPTGFHIVKVEDIRDEKTQSLKDATAEITRALKTEKG
                     KREAAKLADRDREKALSGNDFAKLGQESGVAVNVTRSFTNGEVLPEVGQSPEFYKTAL
                     SMSSKDISPVIEGTNAYYVLKLKQRSEPAVPPFDAARSNIEKSLKESKAHEMLQQKAR
                     SLLEQLKKEKDITKLAQQNGLKLEETGWFLRSAAQLPKVGELSEMRSGGITLSAEKPF
                     PDKIYTQKDNAYIFAFKDSQSADMERFDKEKEQLTNQALEESRQQIMQKFVESLKAKA
                     QIQVHPGSLQES"
     gene            2354..2980
                     /locus_tag="VFN58_05595"
     CDS             2354..2980
                     /locus_tag="VFN58_05595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET8917498.1"
                     /translation="MQVLLVRHGATDWNLQGRCQGATDLDLNEVGVHQAKQIAASLTN
                     ETIHGIYSSNLKRAQQTAQFISLHHQLPVLIETDVRELDHGELEGLTFAEIKENYSQF
                     IQKWRTEPAEIQVPGGERLVDVARRAWNGLNRIVQLHTAQETLVVVSHNFPILGIICR
                     ITGTHLNNYRTYHLDPCGVTRLNHHGGDHWGVTCINNQEYRAETPLTR"
     gene            complement(2977..4539)
                     /locus_tag="VFN58_05600"
     CDS             complement(2977..4539)
                     /locus_tag="VFN58_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET8917499.1"
                     /translation="MSEPSLNPPWFVRKDLDGFFGLMIDNLIQLILIVSLCRELIHLP
                     DTYIFGRILPGAAISILVGNVFYAWQARQLARETGREDVTALPYGINTVSLFAFVFFI
                     MVPIYQETKDPVWAWKVGLVACFLNGVIEIICAFIAESVRRVTPRAALLSALAGIAIT
                     FISMDFTFKIFAQPLVAMVPMAIIFVAYFSHQKFPLGLPGGMVAIAVGTLLGWSLGTM
                     TGKTWNWTYVLAMPVFAGSPLWEALSHPAFLNYFSVIFPMGIFNVVGSLQNIESAEAA
                     GDRYRTFPSLMANGIGSVVAAFFGSVFPTTIYIGHPGWKRLGARSGYSALNGLFIAFL
                     CLTGTIQSVLGLIPLEAGIGILLYIGIIIVAQSFQETPKEHAPAVALGIVPALAAWGL
                     LMVETGLRVAGKSLYVVGIAPFGQSPAMHGVISLQQGFIFTSMILAAIGVALIERRFR
                     TASLWSLAAALLSAVGIIHAYELTPGGVANRFGLLAAPEFFLGYLLLALLFFAAALYA
                     KQREGNAVSVKS"
     gene            5029..5427
                     /locus_tag="VFN58_05605"
     CDS             5029..5427
                     /locus_tag="VFN58_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917500.1"
                     /translation="MHDKVVVIPSLDARLTKLAFFGSSASDIAPLQNPTYKSRFEHAA
                     TTRVYPEIHLEYPPPGKKIYFVMTVHIRENKRTFRIVDYETRIEPNWTSSSHSVGVGV
                     LGPGNWRAGSYEADVYINGERVAMGSFEVY"
     gene            5782..>6293
                     /gene="lipB"
                     /locus_tag="VFN58_05610"
     CDS             5782..>6293
                     /gene="lipB"
                     /locus_tag="VFN58_05610"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET8917501.1"
                     /translation="MMQAMAALLYKDLGRMDYLGALALQERVVELKQRGSLTDVLLFV
                     EHPPVYTLGRGGREANVLAAQEVPVIRTRRGGDVTYHGPGQMVVYPIIDLRSKLRKEV
                     HRYLRNLETSAIRTLNDFGLDGIRRPPYTGIWIDNRKIAAIGIAVRRGITFHGLALNV
                     NTDLSYFSRI"
BASE COUNT         1665 a         1669 c         1627 g         1332 t
ORIGIN      
        1 cgacgatgct tcccggagtg cggcctaaca ttctctttgc ttcggcgccc acagcaagga
       61 cctttttacc gccgcgttcg tttctttgct gaaccgcgac aaccgagggc tcattgcaca
      121 caattccctg gttcttaaca tagatcaggg tgttggcggt cccaagatcg attgcaagat
      181 cgttggaaaa aacgccaaag agggcatcca acatcacaaa actccttcta acctgtgcga
      241 aaaagggcta agcagagaga aaataacaga tttggacttt cctggcaagg taatgaaaaa
      301 acaaaggaaa aatgccgacc ccaaggggcg acaatggttg caactgatgg gccctttttc
      361 tatgatggaa agatagttga ttttgattga aaggttctcc cgctcatgct cgatgtcctg
      421 cgcaagcgca agcgatcgtg gatcattatt ttccttctcg gactcatcat cgtcgtgttc
      481 attgccttct atggtggtaa taagtttcgc gatccgagct cactagacat tgcggaggtc
      541 aatggcgagg tcatcacgca gcgcgaattt gccctgcatt accaaaaaga gttggaacgg
      601 taccgtgaga tgttcaaagg ttcgctcacg cctgaacttg agaagaatct aaatctgagg
      661 ggttcgctgc tcgaacagct catcgaaaag cggctggtac tccaggaagc gcgtcatctc
      721 ggactggcgg cgactgacga agatttgatg acagctattg cgcaggtccc cgacttccag
      781 gtaaacggtc gcttcaataa agaccgttac ctccagctct tacgcgcgag ccgaatcaat
      841 cccggccagt ttgaagacga acagcgagag cagttgacga ttcagcgact ttacagcatg
      901 ctcgtgcacg cagtgcatgt tacggaggcc gaggtgcgcg accgctaccg cttcgaacag
      961 gggaagatca atctctattt tatccgcttg ccgactgcca gctatctttc agaagtaaag
     1021 ccaaccgaag aagagattaa gaaattctat gatcgcaaca aggaattact caaagagccg
     1081 ctgaaagtcc aagtcgaata tctttcctac cccttcgatc ggttctcgtc ctcagcccag
     1141 atcaccgaca aggaggtcga ggattattat caagcgcatc gcgaatcaag attccacaaa
     1201 cccaaggaag tcaaggcgcg ctacattcta gttcgtgtcc cgccaggagc ggatgccaag
     1261 caaaaaaacg acgcgcagac gcgcgcgaat cgcatcctga cagaggcacg agctggaaaa
     1321 gacttcgctc aattggccaa aaaagagtcg gaggactcga ctgcggccaa gggaggcgag
     1381 gtgggttggc tgacacaagc gcaattgcct tcgccgctcg acaaagccgt tttcaacctg
     1441 gccaagggcg agattagcaa cgtcatcgag agtcctacgg ggtttcatat cgttaaggtc
     1501 gaggacattc gagacgagaa aacccaaagt ttgaaagacg ccactgcgga gatcacccgc
     1561 gccctcaaga cagaaaaagg caagcgggaa gccgcgaaac tcgcggaccg agaccgagag
     1621 aaggcgctct ccgggaacga cttcgccaag ttgggccaag agagcggcgt tgcggtcaat
     1681 gtgactcgct cgttcaccaa cggtgaagta ctgccggaag tcgggcaatc gccggaattc
     1741 tataagaccg ccttatccat gagttccaag gacatcagcc ctgtcatcga gggaacgaac
     1801 gcctattatg ttctaaagct caaacaacga agcgagccgg cggttccacc cttcgatgcg
     1861 gcacggtcca acattgaaaa aagcctgaag gaatccaaag cgcacgaaat gttgcagcaa
     1921 aaggcgagaa gtttgttgga acagttaaag aaagagaaag acatcaccaa acttgctcag
     1981 cagaacggct taaaattgga ggaaaccggg tggtttctcc gcagcgcggc tcaacttccc
     2041 aaagtcggag agctttcgga aatgaggtcg ggcggaataa cactgtcggc agagaaacct
     2101 ttccctgata agatttacac gcaaaaggat aacgcctata tcttcgcctt caaagatagc
     2161 cagagcgccg atatggagcg cttcgataag gaaaaggagc agctcacaaa tcaagcgtta
     2221 gaagaaagcc gtcaacaaat catgcaaaaa ttcgtcgaaa gtctcaaggc caaagctcag
     2281 attcaggttc accccggctc cttgcaagaa agctagcgct ttcttctcgt taatttcgct
     2341 caggattgac attgtgcaag tcctattggt tcgtcacggt gccacagatt ggaatttgca
     2401 gggtcgctgc caaggggcga cggatctgga tctgaatgag gtcggagtcc accaggcgaa
     2461 gcagatcgcg gcgtcactga ccaacgaaac gatacatggt atttactcaa gcaatctgaa
     2521 gcgcgcgcaa cagacggctc aattcatcag tctgcatcat caactccccg tgcttatcga
     2581 gaccgacgtt cgcgaacttg accatggcga attggaaggt ctgacattcg cagaaatcaa
     2641 agagaactac tcacaattta ttcagaaatg gcgaaccgag ccggcagaga tccaggttcc
     2701 aggcggcgag cgactcgtgg atgtcgcgcg cagagcctgg aacggattga atcgaatcgt
     2761 gcagcttcat accgcccaag aaaccctagt cgtggttagc cacaacttcc cgatcctggg
     2821 catcatctgc cggattacag gcactcattt gaacaactac cgcacttacc acctcgaccc
     2881 ttgcggggtt acccgtctca accaccacgg cggcgatcac tggggagtca cctgcatcaa
     2941 taatcaagag tatagggcag agacaccgct tacacgctag cttttgactg atacagcatt
     3001 gccttccctt tgcttcgcgt acaacgccgc ggcgaagaag agcagcgcca acagcaaata
     3061 gcccagaaag aactccggcg ccgcgaggag accgaagcga ttggcaacgc cgccgggggt
     3121 gagctcgtag gcgtgaatga ttcctacggc cgagagaagc gcggcagcca aagaccaaag
     3181 acttgccgtc ctaaaccgcc gttcgatcaa cgctaccccg atcgccgcaa gaatcatgga
     3241 agtaaagatg aacccttgct gcagggaaat cacaccgtgc atcgccgggc tctggccgaa
     3301 tggcgcaatt ccgactacgt agagcgactt gccggcgact ctgagccccg tctcgaccat
     3361 caacagcccc cacgcggcca atgccggaac gatccccaac gccaccgccg gagcatgttc
     3421 cttcggcgtt tcctgaaacg actgcgcgac gatgataatt ccgatgtaga gaagaatccc
     3481 tattcctgct tcgagaggaa tcaagcccaa gacggattga atcgtcccag tgaggcaaag
     3541 aaaggcgatg aacaacccat tcaatgccga atagccggac cgcgcgccga gtcgtttcca
     3601 accgggatga ccaatataga tagtcgtagg aaagacgctg ccgaagaatg ccgccacgac
     3661 ggagccaatc ccattggcca tgagcgatgg aaaggtgcgg tagcggtcgc ctgcggcttc
     3721 ggcgctttcg atgttctgca acgaaccaac gacgttaaaa atccccattg gaaaaatgac
     3781 cgaaaaataa ttcagaaacg ccggatgtga gagcgcctcc caaagcggac taccggcaaa
     3841 gaccggcatg gccaaaacat aggtccaatt ccaggttttc cctgtcattg tacccagtga
     3901 ccaaccaagc aaagtgccca ccgcgatcgc caccatgccg ccaggcaagc caaggggaaa
     3961 tttctggtgt gaaaaatacg ccacaaagat aattgccatc ggcaccatag caaccaaagg
     4021 ctgggcaaag attttgaaag taaaatccat cgaaataaat gtaatggcga tgcccgccag
     4081 cgccgacagc aacgctgctc gcggcgtgac gcgacgcacg ctttcggcga taaacgcaca
     4141 gataatttcg atgacaccgt tcagaaaaca agcgacgagc ccgactttcc atgcccagac
     4201 cggatctttg gtctcctggt aaatcggtac cataatgaag aatacaaaag cgaagaggct
     4261 gaccgtgttg atgccatagg gcagcgcggt gacgtcttcc cgtcccgttt cacgtgccag
     4321 ctggcgcgcc tgccaggcgt agaagacgtt gccaaccaga atcgaaatcg ccgcccccgg
     4381 caaaatgcgc ccgaagatgt aggtatccgg caaatggatc agctcgcggc agagactgac
     4441 gatgagaatg agctggatca gattatcgat catgagacca aaaaacccgt ctagatcctt
     4501 acggacaaac cacggtggat taagtgaggg ttctgacatg gaaacggctc tgcgctgttg
     4561 aattcggaag aatgtagctg cgcctgctct tctctgtcaa tccaacaaag attacccggc
     4621 tggtcgactg ttggagcttt gtgtcacgca agttttgacg gcccattttt ttgggtgcac
     4681 gcgtttacca acgaacgctc aaaatccctg aacggttgtt gtgaatctcc aaacctgaca
     4741 ccggccaagt ttcgtcttct ccggccactt ttgtactgaa tcgggttctt ttcaaggcag
     4801 aacccgtgga agagcgatga ttctaccaaa cccgcttgct ggccttttca ttgcatttcc
     4861 ttcgtgcggg agtaactaac ataaggaggg gtaatgttta atcggaaacg atcctgtatg
     4921 ccgctgcttc tcggacttct acttttatca accacggggt gtagctcttc attaagaaac
     4981 tcgtcgtcgg aacctaagac cgcttcctcg gtgcctgagc cttcgatttt gcatgacaaa
     5041 gttgtagtga ttccatcact ggacgcacgt cttacgaagc tcgcgttttt tggcagcagc
     5101 gcttccgaca tcgcacctct tcaaaacccg acatacaaaa gccgattcga acacgcggct
     5161 actaccaggg tctacccgga aattcacctc gagtatcccc cgcccggaaa gaaaatctat
     5221 tttgtaatga ctgtccatat ccgagagaac aagagaacgt tcagaattgt ggactacgag
     5281 accaggatcg agcccaactg gacttcgtct tcccattcgg tcggtgtagg ggtattgggt
     5341 ccgggaaatt ggcgtgcggg cagctacgag gcggatgtct acatcaacgg cgagagggtt
     5401 gcgatgggtt cctttgaagt ttactaatca cgcaacggtc cgaaactcca tggatcagat
     5461 tcagtgatcg tttgcgcttc ttctgctcca aggtttacag ccgtagagac cttagaactg
     5521 tttcaagtac gaaagcgcct ttcgagcaac gatagttctt gacgatcttg caggcgttta
     5581 ctacgtttag acgaaacccg gcgtctgctt gggtttagct cgagatcttc acaagctttc
     5641 ccaatccttg tctggagaga tggcaggctt tgccgggatt tctgccttct tgttctcgca
     5701 ccattcgatt agtacgcatt ctggtcgccg aatatccaac gtagtttacc aacttgaaag
     5761 atcgcagcat ggacgataag aatgatgcaa gcgatggctg cgctgcttta taaagacttg
     5821 ggccggatgg attaccttgg agcgctggcg ctgcaggaga gagttgtaga actgaagcaa
     5881 cgtgggtctc taaccgacgt actgctcttt gtcgagcatc cccccgttta tactcttggt
     5941 cgcggagggc gggaggccaa cgtgcttgct gctcaagaag ttccggtcat taggacacgc
     6001 cgcggtggag acgtgacgta ccacggtccc ggccagatgg tggtgtatcc catcatcgat
     6061 ctgcgctcga aattgcgcaa ggaggtgcat cgttatttaa gaaacttgga gacgagcgcg
     6121 atccgcacgc ttaacgattt tggtctcgac ggaattcgcc ggcccccgta caccggtatt
     6181 tggatcgaca acagaaagat cgcagccatc ggcatagccg tacgtcgcgg tattactttt
     6241 cacggcttgg ccttgaatgt gaataccgac ctctcctatt ttagtcgcat tat
//
LOCUS       DASSWZ010000231         5599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1154489, whole genome shotgun sequence.
ACCESSION   DASSWZ010000231 DASSWZ010000000
VERSION     DASSWZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154489"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="VFN58_05615"
     CDS             <1..1649
                     /locus_tag="VFN58_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET8917502.1"
                     /translation="RLSLDELVAKVREGNPEAGPAVITVKSNPAASVIINLGRENTLF
                     INPYTGELLGGLSTTHNVLHEIVDWHRWLGTEDESRAIGKAITGACNLAFFWLAVTGV
                     YLWWPHNWNWRGLKTSLVFQRRLSGKARDWNWHNVIGFWSSSVLVVLTLTAAVMSYPW
                     ANDLLYTLTGSEPPPRAQGPASPPQRASRGSGNTEEPRIAKPMANLEALLATAERQVP
                     GWTMLMMRFPPRPDGPVTVSISEPSAPHNFARSQLTLNRATADVVKWEPYSENSTGRK
                     LRSWFRGLHTGEAFGFFGQTIAGLASLGGCFLVWTGLAMAWRRSRSWGREVEEPSIVQ
                     SALSNNSGSIEISQDLQLKGEKSPMDVDILPAESSQLGHATNRNGAGAHKVEFMESHA
                     DQNSVLILFGTVTGNAESLAQLTAEVIARRGFKVRVKDMAHYAVDALSHEKCVVIITS
                     TYGNGEPPDDAAPFWKGVVQRDGLNLRGVKFSVLALGNSTYDHFCKCGRDLDAALERH
                     GATRLYPRVDCDVDYDVPAKRWTEGVIASLQRDHSASAAA"
     gene            1649..2350
                     /locus_tag="VFN58_05620"
     CDS             1649..2350
                     /locus_tag="VFN58_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HET8917503.1"
                     /translation="MKEGVTSMLMFQSASAAILILSLINTADAHYVWLERDGEGSAYA
                     YFGEWVDDIREKAGGLLDRIKAPRAFLGTSNTPLAIKRHEDKLEIVAKGKGDLRLVED
                     GIPPRDDSEKGGKTRTIYYAKAGRSETAAKLDLELVPTVAEGNTLTLLLYGVPLAKTE
                     VTVIGPPKWEKHLTTDEQGRIAMPTPWSGRYVLEVTHFEAKAGGSENDKFDRTRHITS
                     LSFIQRKGLSWSAKR"
     gene            complement(2909..3697)
                     /locus_tag="VFN58_05625"
     CDS             complement(2909..3697)
                     /locus_tag="VFN58_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET8917504.1"
                     /translation="MPQSGLGLIDSHAHIQGKEYTGETAAVIQRAGEAGVEQIIVVGG
                     AGDMSSNTAAVALAESCANLYATVGMHPHDAKDVGEEELQKLKKLVAYPKVIAVGETG
                     LDYYYNHSPREVQRRVFAQFIRVAAETGLPLVVHERDAASEAVELLRSEGAGKIRGVI
                     HCFTGDYDAARNYLDLGFYLSFTGIVTFKNAQALRDVVRRVPLDRMFVETDSPYLTPV
                     PYRGKRNEPAYVKLVAETVARVKNMTLEEVAHTTTQNVRRLFRI"
     gene            complement(3711..5249)
                     /gene="metG"
                     /locus_tag="VFN58_05630"
     CDS             complement(3711..5249)
                     /gene="metG"
                     /locus_tag="VFN58_05630"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET8917505.1"
                     /translation="MDRIYITTPLYYVNAEPHLGSAYTMIITDTLARYYRAKGWEVFY
                     LTGTDEHGDKIAQAAAQAGVDPKPFTDQISATFRAAWDSCGFSYDHFIRTTDAYHVRY
                     VQELLTKVYEAGDIYFDRYGGLYCFGCERFYTEKELVDGKCPDHRTVPELIEEENYFF
                     RMGKYQDRLLRYLEEHPDFIRPEGFKNEVVAMLREPIGDLCISRPKSRLTWGIEIPFD
                     RRYVTYVWFDALINYVSALKHRGEDFFEKFWPLAEHFIGKDILKPHGVFWPTMLMAAG
                     LPLYKHLNVHGFWTSEGQKMSKSLGNTVSPLEMRERIGMDAFRYFVLRESVFGQDADF
                     REDALTARYNADLANNLGNFVSRVLAMQNRYFSGVVQPLSGDWSKEDRTLKDKFVQAE
                     SELNRHMAELQFHRALESIWTAIDYANRYIVQTAPFTLFKDPARRMRVGEVLHHLLEA
                     IRQLARLLAPFMPDTSKELQRLLGLKEGDAAYRVSWGQCFSPGHKIDGPTVLFPRIEL
                     PSEA"
     gene            complement(5249..>5599)
                     /locus_tag="VFN58_05635"
     CDS             complement(5249..>5599)
                     /locus_tag="VFN58_05635"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HET8917506.1"
                     /translation="RRREWVRLISDLGAGDYRAATDAAEILAGSKEDSLRFLEWVESW
                     FRDLLVYSTTRNPQVVVNIDMLPQIQQRSAATDCERLFSRIAEAKAAIGGIQRNLNRR
                     MVIEDLLLNTVEAS"
BASE COUNT         1305 a         1533 c         1529 g         1232 t
ORIGIN      
        1 aacgcctgag cttggacgag ctggtggcga aagtgcgcga aggcaatccc gaggcgggtc
       61 cggcagtcat cacggttaag tcgaaccccg ccgcttcggt catcatcaat cttggcagag
      121 agaacaccct ttttattaat ccttacaccg gcgaactgtt gggcggcttg tcgacgacgc
      181 acaatgtgct ccatgaaatc gtcgactggc atcgttggct gggaaccgag gacgagagcc
      241 gcgccatcgg caaggccatc accggcgctt gcaacttggc gtttttctgg ctggccgtca
      301 ccggcgtcta tctttggtgg ccgcataatt ggaactggcg cggtctgaaa accagcctgg
      361 tatttcagcg acgcttgagc ggtaaggcgc gggattggaa ctggcacaat gtcatcgggt
      421 tctggtcgtc gagcgtgttg gtggttctta ccttgactgc agcggtcatg tcttatccct
      481 gggccaacga tctgctttat acgcttacgg gaagcgaacc accgcccaga gctcagggtc
      541 ccgcgagccc gccgcaacgc gcgagtcgag gctcgggtaa cacagaagaa ccaaggatcg
      601 ccaagccgat ggcgaacctc gaagctcttt tggctactgc agaacggcaa gtgccaggtt
      661 ggactatgct catgatgcgt tttccacccc gtccggacgg tccggtcacc gtttcgattt
      721 ccgagcccag cgcgccacat aattttgccc gctcgcagtt gacccttaat cgcgccacag
      781 cggatgtggt gaagtgggag ccctattcgg aaaacagcac aggaagaaag ctcaggagtt
      841 ggtttagggg gcttcatacc ggcgaggcct ttggattttt cggccagacc atcgccggtc
      901 tggcttccct tggcggctgc tttttagttt ggaccggttt ggccatggca tggcggcgct
      961 ctcgttcctg gggtcgggaa gtggaagaac cgtcgattgt ccaatcggct ctgtcaaaca
     1021 attcaggatc gatcgaaatc tcacaagatt tacaactcaa aggagaaaaa tcacctatgg
     1081 atgtcgacat tttgcctgcg gaatcttctc aactaggtca tgcaacgaac aggaacggag
     1141 caggcgccca caaagttgag ttcatggaaa gccatgcgga tcagaattct gtgttgatcc
     1201 tctttggcac cgtgaccggt aacgccgaat cactggcgca actcaccgcc gaggttatcg
     1261 ctcggcgggg gttcaaagtg cgcgtgaaag acatggccca ttacgccgtc gatgcactgt
     1321 cccatgaaaa gtgcgtcgtc atcatcacca gcacctacgg caatggcgag ccccccgacg
     1381 atgctgcgcc gttttggaaa ggggtggtgc aaagagacgg cttgaatcta cgcggggtga
     1441 aattttccgt cctggcgctg ggcaattcca cctatgatca cttctgcaag tgcggccgcg
     1501 acctcgatgc agcattggag cgccacggcg ccacgcgcct ttacccgcgc gtcgattgcg
     1561 acgtggacta tgatgtgccg gccaagcgtt ggaccgaggg ggtgatcgcg agtttgcagc
     1621 gagaccatag cgcttccgca gcagcctgat gaaggaagga gtgacgtcga tgttgatgtt
     1681 ccaatccgcc tctgccgcga ttctgatatt gtcgctgatc aataccgccg atgctcatta
     1741 tgtgtggctg gaacgggacg gcgaagggtc cgcctacgcc tactttggcg aatgggtcga
     1801 cgacatccgt gagaaagctg gtggtctgct ggatcgaatc aaggcaccgc gcgcgtttct
     1861 tggcacaagc aacacccccc tggctatcaa acgacacgaa gacaagctcg agattgtagc
     1921 caaagggaaa ggcgacctac gattggtgga agatggaata ccaccgcggg acgacagtga
     1981 aaagggtggc aagacccgga caatctatta tgcaaaggcc ggtcgatcgg agaccgcggc
     2041 caaactcgac ttggaattgg tcccaaccgt cgccgaaggc aacaccctta cccttcttct
     2101 ctacggcgtg cccctcgcca agacggaagt taccgtaatc ggtccgccga agtgggaaaa
     2161 gcatttaacg accgacgagc agggtcggat cgccatgccc accccctggt ctggccgata
     2221 cgttttggaa gttactcact tcgaagcaaa agcgggtggc tccgaaaatg ataagttcga
     2281 tcgcacccgc cacattacat cactctcgtt catacagcgg aaggggcttt cgtggtccgc
     2341 caaacgctag tggcatggtt aaaaaaagcg aggtctgcaa aatcatgagt taacgcgcgt
     2401 cgatgaaata agccagcggc cagaggttgt taatccagag cccgcggttt cgccaaccta
     2461 ctgcttaaca taaggagaga gtcacgaagg atggtatttc ggaaaaaaaa tgggaagtca
     2521 ctattggtca aggagcactg gtacacgtac actacgaaac ggcctcaacc gaagtcatca
     2581 atgacggttt caaggggttg gcacgggaac ttcgcgaggt cgccgaccag atcgaagcga
     2641 tcgtcccgaa gaccaagcag caaacatgac aattttaacc attcccccct tcttaggcgc
     2701 ctcctaggga agggttgcgg gataggagtt caccctctgg cgcatgtcag gggcgggctc
     2761 cgccaattgg ggcggaccgc aaaaacagcc ttggaaagac tggaatagag ggacttacca
     2821 gcgcagacac catgaaaagg tcatgaattc taattcttct tgtgaatctg ctcgggtgac
     2881 aattgtgaaa ccggcgacgt ttcgtttctt agattcgaaa aagccgtcgg acattttggg
     2941 tcgtcgtatg agcaacttcc tccagggtca tgttttttac acgtgcaacg gtttctgcga
     3001 ctaactttac gtaagccggc tcgttgcgct tccctctata aggaacgggc gtgagatagg
     3061 gcgaatcggt ttccacgaac attcgatcca gcggcactct ccggacgaca tcccttagcg
     3121 cctgcgcgtt cttgaaagtg acaatgccag taaaggagag ataaaacccc aaatcgaggt
     3181 agttccgagc agcatcataa tcacccgtga agcagtggat cacaccgcgg atttttccgg
     3241 cgccttcact acgcagtagc tcgacggctt cgcttgcagc gtcccgctca tgaacgacaa
     3301 gtggcaatcc ggtctctgcc gccacgcgga tgaactgcgc gaaaacccgg cgctgaactt
     3361 cgcgcggcga atggttgtaa tagtagtcca gaccggtttc gcctacggca atcaccttcg
     3421 ggtaagcgac gagttttttt agtttctgga gttcctcttc gccgacgtcc ttcgcatcgt
     3481 ggggatgcat accgacggtg gcatagagat tggcgcacga ctcagcgagg gcaaccgcgg
     3541 cggtgttgct cgacatgtca ccggcgccgc cgacgactat aatttgctca acgcccgctt
     3601 caccggcgcg ctgaataacc gccgcggttt caccggtgta ctctttcccc tgaatatgtg
     3661 cgtggctatc gatcagccct aaaccacttt gcggcatcac gggcaaccca ctaagcttcg
     3721 ctagggagtt caatgcgcgg gaaaagaaca gttggaccgt cgatcttatg acccggtgag
     3781 aaacactggc cccaagagac gcgatatgct gcatcgcctt ctttgagccc aaggagacgt
     3841 tgcagctcct ttgaggtgtc gggcatgaac ggtgcaagta accgagcgag ctgacgaatg
     3901 gcttcgagca gatgatgaag aacttccccg acccgcattc gccgcgcagg atccttaaac
     3961 aacgtaaacg gtgcggtctg cacaatatag cggttagcgt aatcgatagc agtccaaatc
     4021 gattcaaggg cgcgatgaaa ctgaagttcc gccatatgac gattcagctc tgactctgcc
     4081 tgaacaaatt tatctttgag cgtccgatct tcttttgacc agtcaccact caatggctgg
     4141 acaaccccgg agaaatatct gttttgcatc gccagtactc ggctgacaaa attcccgagg
     4201 ttgttggcca aatcagcgtt gtaacgcgcc gtcagagcat cttccctaaa gtccgcgtct
     4261 tgtccgaaga cgctctcccg cagcacgaag taacgaaagg catccatgcc gatcctttcc
     4321 ctcatctcca gtggcgagac ggtgtttccc aaacttttgg acattttctg gccttcactg
     4381 gtccagaagc catgcacgtt caagtgcttg tataacggca gcccagccgc catcagcatg
     4441 gtcggccaaa agacgccatg gggtttgagg atgtctttgc cgatgaaatg ctcggcaagg
     4501 ggccaaaatt tttcgaagaa atcttctccc ctatgtttca gcgcactcac atagttgatg
     4561 agcgcgtcga accatacgta agttacgtaa cgccgatcga aagggatttc gatgccccag
     4621 gtgagacggc tcttgggacg ggagatgcac agatcgccga tcggttcgcg cagcatcgca
     4681 accacttcat tcttgaagcc ttcgggtctg atgaagtcgg gatgctcttc caagtagcgc
     4741 aatagccggt cctggtattt gcccatgcga aagaaatagt tctcctcctc gataagctcc
     4801 ggcacggtcc ggtgatccgg gcatttgccg tccactaatt ctttctctgt atagaagcgc
     4861 tcgcagccga agcaatagag cccgccatag cggtcgaagt agatatctcc ggcttcgtaa
     4921 actttcgtca agagttcttg cacataacgc acatgatagg catccgtggt acgaataaag
     4981 tgatcgtacg aaaagccgca actatcccag gctgcccgga aggtcgcgct gatctgatcc
     5041 gtaaatggct tgggatccac gccggcctgc gcagcggcct gagcgatttt gtcgccatgc
     5101 tcgtccgtac cggtaagata aaagacttcc caaccttttg ccctgtaata gcgcgccagc
     5161 gtatcagtga tgatcatcgt gtacgcactg cccaagtgag gttcggcgtt aacgtaataa
     5221 agtggagtgg taatgtaaat gcggtccatt aactagcctc aaccgtgttc agtagcaaat
     5281 cctcgatcac catgcggcgg tttagatttc tttgtattcc tccgatcgct gctttagcct
     5341 cggcaatccg agagaaaagg cgctcgcagt cggtggcagc tgatcgctgc tgaatctggg
     5401 gtaacatatc aatattgacg acaacttgcg ggttccgcgt ggtgctatat accagcagat
     5461 cgcgaaacca actttcaacc cattcgagaa acctcaagga atcttctttg cttccggcaa
     5521 gaatctctgc ggcatcggtg gccgcacggt aatctcccgc acctaaatcg gaaatcagcc
     5581 gcacccattc tcgccgcct
//
LOCUS       DASSWZ010000232         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_419076, whole genome shotgun sequence.
ACCESSION   DASSWZ010000232 DASSWZ010000000
VERSION     DASSWZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419076"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..978)
                     /locus_tag="VFN58_05640"
     CDS             complement(166..978)
                     /locus_tag="VFN58_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET8917507.1"
                     /translation="MPDNKLIIDNINEEEGVAWLTLNRPEKKNALSIALLDELAHLLR
                     TIGENDKIRCIVTRSAGDSYSSGRDLYDMRSQNNRRRNRGEGGVAEIVSIMRKLPQVT
                     VAAVRGWCLGGGLAMINGHDLVISADTAKFGMPEIIRGSYGATATPSLFHGGIPIKKA
                     FYISLTGRNLTGVEAERIGLVSHVVPESELDSYVETLAKEIASRNGATLESAKIAAYM
                     QKDLPFHMALQADDLVQHRMRYYTNPLSDVEGYLHSQKGGGTVKYVKPEDRK"
     gene            complement(1109..2119)
                     /locus_tag="VFN58_05645"
     CDS             complement(1109..2119)
                     /locus_tag="VFN58_05645"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917508.1"
                     /translation="MNHSFTVIDADAHHLDGPAYKDYLPERFRARSGPYFPSFGWDIF
                     LNGTTGRKPANPEEYCIDLDAENIGDAVAYPSNALAIGLVRELDLAVELAKAYNNWAY
                     DFCKSTGNRVNYAAVIAPQVVPEAVKEIRRAVTEKGAVGVMMPTYVQSGLDLGQPEFD
                     SIYAAAQELGVPVGFHATAQVAVGNTRFHKYLGVHMTSHPFEQMLSIVSIISNGVLDR
                     FPKLRVAFLEAGVGFLPYWMERFDEKYLKRKSEMEPLKLLPSEYVKTNRCYFTCEGEE
                     SALPLVIEQFGDDCMMYASDYPHWDTEWPHTVSQVVGRKDISDKTKQKILADNAKAFY
                     RF"
     gene            complement(2186..3871)
                     /locus_tag="VFN58_05650"
     CDS             complement(2186..3871)
                     /locus_tag="VFN58_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8917509.1"
                     /translation="MVMMNPAQWMFLFTGVALAYLVLPPFFFILHTSLVVDRGLQAGS
                     FTIQHFMNIVESLGDVKTLLANSMIFSVGSAGLAICYGTLLAWLAERSNAPFRTLAYI
                     TAYISFAIPGIIKVVGWIMLLGPKAGILNAAVMPFTGGPLLNIFSMQGMILVESFLWI
                     PIVFLLMSTPFRSMDPSLEEAATTAGSTSWQVFRKVTFPLALPSVLAVLILTFIRSLE
                     AFEIPALIGIPAGVEVLTTKIYLQIRGGLIPRYGEASAYSILLIVMVALGLIPYYRIT
                     SKTYKFTTISGKAYRPHRIDLGRWRWLGGCLMLVLPMLQFFPIAAITWSSFLPFAQAP
                     SWRAVSLLSLNNYRTAFADSSIVGSVFNSLTVSITSATIAIMITFFAAWLIVRASIKS
                     RWILDQMAMLPLVFPGIVMGIAILKMYLMIPLPVYGTIWILVLAFIARYLPYGIRFSH
                     SALLSLHKELEEGAMVSGASWFQMVRQVVMPLIMPALLAGWIYIFLITFRELSIALLL
                     YSPGSQVVAVKIWELWDNGHVGELAAFSLVIAIGTVIVGSIFLRLAQRYGLQD"
BASE COUNT          985 a         1074 c         1145 g          849 t
ORIGIN      
        1 cggatctcac cgaaacgcag aagcgtaaga ttctttacga caacgcggtc aagtttttcg
       61 gcgagccgta agcttcccgt taaacaacgg tcgctcacca atgaaagcga ataagctctc
      121 gattcaccgc gttgcggact ggacgagcat tgccgggcga gttacttact tcctatcctc
      181 tggctttaca tatttcaccg tgccgccgcc tttttgcgag tggagataac cttccacgtc
      241 gctcagcggg ttggtgtagt agcgcatgcg gtgttggacg agatcgtcgg cttggagggc
      301 catgtgaaag ggcagatcct tttgcatata agcggcgatc ttagcgctct ccagagtggc
      361 gccgttgcgg ctggcgatct ctttggcaag ggtttcaacg tagctgtcga gttcactttc
      421 agggacgacg tgggagacga ggccgatgcg ctccgcttcc acacccgtga gattcctccc
      481 ggtgagcgag atataaaagg ccttctttat cggtatgccg ccgtgaaaga gtgacggcgt
      541 tgccgtggcg ccgtagctgc cgcgaatgat ttccggcatg ccgaacttgg cggtgtcggc
      601 gctgatgaca agatcatggc cattgatcat ggccaatccg ccgcccaggc accagcctct
      661 gaccgccgcc acagtgacct gaggaagttt acgcatgatg gagacgatct ccgccacacc
      721 gccctcgccg cgattgcggc gccggttgtt ctggcttctc atgtcgtaaa gatcgcgccc
      781 ggaagaataa gaatcgcccg cgctcctagt cacgatgcag cgaatcttgt cattctcgcc
      841 aatggttctg aggagatgag cgagctcatc tagaagagct atgctcaggg cgtttttctt
      901 ttccggccgg ttgagtgtca gccaagcgac gccttcttcc tcgttgatgt tgtcgatgat
      961 gagtttgtta tcgggcatgg tttgtctccc taggtaagaa tttgttccaa ttgttccagc
     1021 cgttccaatc gttcaagttg tgcagacaaa aagtcacaag ctgacactgg cgctggacta
     1081 ccgtgcagat tttggagata gcacactatt agaaacggta gaaggccttg gcgttgtccg
     1141 cgagaatctt ttgtttagtc ttgtcagaga tgtctttgcg gccaacgacc tgactcaccg
     1201 tgtggggcca ctcggtatcc caatgggggt agtccgatgc gtacatcatg cagtcatcgc
     1261 cgaattgctc gatcaccagc ggcaaagcgg attcttcgcc ttcacacgtg aagtagcagc
     1321 gattggtctt gacgtactcg ctggggagca gcttgagcgg ctccatttct gattttctct
     1381 tgagatattt ttcgtcaaag cgctccatcc agtagggcaa aaagccgaca ccggcttcga
     1441 gaaaggccac cctgagttta ggaaagcgat cgagcacgcc gttcgatatg atggagacga
     1501 tggaaagcat ctgctcaaaa gggtgcgaag tcatgtggac acccaggtac ttgtgaaagc
     1561 gcgtgttgcc gaccgcaact tgggcggtgg cgtgaaagcc gaccggaaca cccagttcct
     1621 gcgctgcggc gtaaatggaa tcgaattcgg gttggcctaa atccagtcct gactgcacgt
     1681 aggtgggcat catgacaccg acggcgccct tttcagtgac ggcgcggcgg atttccttga
     1741 ccgcttccgg caccacttgc ggcgcaatca ccgccgcgta attgactcga ttgccggtgc
     1801 tcttgcagaa atcatacgcc cagttgttgt aggccttcgc cagctcgacg gcgaggtcta
     1861 gctcgcgcac caagccgatg gcgagggcgt tactgggata ggcgacggca tcgccgatgt
     1921 tttccgcgtc aaggtcgatg caatattcct cggggttcgc gggttttcgg cccgtcgtgc
     1981 cgttcaaaaa aatgtcccat ccgaaggaag ggaaataagg cccgctacgc gcgcgaaagc
     2041 gctccggcaa atagtcttta taggccggcc cgtccagatg atgcgcatcc gcgtcgatga
     2101 cagtgaaact atggttcaca atttatcctc cgaaacgact ggaacgattg gaacgttttg
     2161 aacgattgga acatctcaac ggagatcagt cctgcagccc gtagcgctgc gcgagcctta
     2221 aaaagatcga acccacgatg accgtgccga ttgcgatgac aaggctgaaa gccgccagtt
     2281 cgccgacgtg gccgttatcc cacagttccc agattttcac cgccacgacc tgtgaacccg
     2341 gtgaataaag caaaagggcg atggagagct ccctgaacgt aatcagaaag atgtaaatcc
     2401 aaccggcaag caacgcaggc ataatcaagg gcatgaccac ttgccgcacc atctgaaacc
     2461 agctcgcgcc actgaccatc gccccttctt ccaattcctt atgcaaactc agcaacgccg
     2521 aatggctgaa gcgaatacca taggggaggt aacgggcgat gaaagccaag actagaatcc
     2581 agatcgttcc gtatacaggc aaagggatca tgagatacat cttcaaaatg gcgattccca
     2641 tcacgatccc cggaaacact aacggcaaca tcgccatctg gtccaggatc cagcgcgatt
     2701 tgatcgaagc gcggacgatc aaccacgcgg caaaaaaagt aatcatgatg gcgatggtcg
     2761 cggacgtgat gctcacggtc aggctgttga aaacggagcc gacgatgctg ctgtcggcga
     2821 aggcggtccg ataattattg agactcagca acgaaaccgc gcgccaagac ggcgcttggg
     2881 caaaaggcag gaacgaggac caggtaatcg cggcgatggg gaaaaactgg agcatcggca
     2941 aaaccagcat gagacagccg cctaaccagc gccagcggcc caaatcaatc cggtgtgggc
     3001 gatacgcttt gccgctgatg gtcgtgaact tataggtctt gctcgtgatt cgatagtaag
     3061 gaatgagacc gagagcaacc atgactatga ggaggatcga ataggcgctg gcctcgccgt
     3121 atctgggaat aaggccgccc cggatctgaa gatagatctt ggtggtaagc acctcgaccc
     3181 cggcgggaat gccgatcaac gccggaattt cgaaagcctc gagggatcga atgaaggtta
     3241 gaatcaatac ggccaggacg ctcggcagcg ccaacggaaa ggttaccttg cgaaagacct
     3301 gccagctggt gctcccggcg gtggtggcgg cttcttccag agatgggtcc attgaacgaa
     3361 acggagttga catcaacaga aagactatgg gtatccagag aaaactttcc accagaatca
     3421 tcccttgcat ggaaaagata ttgagcagtg gtccaccggt gaaaggcatg accgcggcat
     3481 tgagaatccc tgccttgggg ccgagcagca taatccagcc gacgacttta atgatcccag
     3541 gaatggcgaa tgaaatgtag gctgtgatgt aggccaatgt gcgaaaggga gcgttggagc
     3601 gctccgcgag ccacgcaagg agggttccgt aacagattgc cagaccggcg ctccccaccg
     3661 agaagatcat cgaattggcg agtaaggtct taacgtcgcc gagggattcg acgatgttca
     3721 tgaagtgctg gatcgtgaag ctcccggctt gaagaccgcg gtccaccacg aggctggtat
     3781 ggaggataaa gaaaaatggc ggcaggacaa ggtaggccag cgctacgccg gtaaaaagaa
     3841 acatccattg ggcgggattc atcattacca tgactttagc cctctcaacg cattagcggt
     3901 ctcgggttaa atagcctcgc cttcatgcga gacggcaact caaatactcg aaacagacct
     3961 tcgcctgcta tatatcaaat ccgttgggca agacaattac gaatcaccaa ccgatcgctg
     4021 gccgaaatat ccttcgcggt gctcaagacg acg
//
LOCUS       DASSWZ010000233         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1381538, whole genome shotgun sequence.
ACCESSION   DASSWZ010000233 DASSWZ010000000
VERSION     DASSWZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381538"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFN58_05655"
     CDS             <1..289
                     /locus_tag="VFN58_05655"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET8917510.1"
                     /translation="VEDAGAFAVVLEGIPLDLAKQISERLTIPTIGIGAGPHCDGQVL
                     VIHDMLGLFDDFTPKFVKRYADLQEVMTGAVKSFIADVRERKFPGEEHSFK"
     gene            398..1243
                     /gene="panC"
                     /locus_tag="VFN58_05660"
     CDS             398..1243
                     /gene="panC"
                     /locus_tag="VFN58_05660"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET8917511.1"
                     /translation="MQIIEHISEMQAWSESTRRDGRRIVFVPTMGFLHDAHLSLVRDG
                     KKRGDRLVVSIFVNPAQFGPNEDFAAYPRDFERDRGLLEREHADVLFHPSVREIYPEG
                     YQTHVGVDILSALLCGAHRPGHFQGVATVVAKLFNIVRPHVAIFGEKDYQQLQLIRRL
                     VRDLNFEIEIVGHPIVRETDGVAMSSRNIYLSPEERQAALCLSRALVRAACMVQRGET
                     RGRVITEAVRAGIEKEPLARVEYVTLCDSATLEPVEEIQESALLALAVRIGKTRLIDN
                     QILKP"
     gene            1252..1905
                     /locus_tag="VFN58_05665"
     CDS             1252..1905
                     /locus_tag="VFN58_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET8917512.1"
                     /translation="MADDTQKRMKINVREILQRYFLAGLLVFLPVVITLWFLGWVIGL
                     MDGLLDVLPARLHPNSYLPFAIPGLGAAFTIALILFLGVLTRGVATRRFLAAWDRIFV
                     QIPVFRGVYTAVQKLVQTFLGQSQTNRQVVMIEYPRKGIYAVGFAMGRAWHELEKKNE
                     APLVNVFVPTTPNPTSGFYLLVPANEVAPLNISMEEALKLITSGGLITPDDRNKNHG"
     gene            1898..>2046
                     /locus_tag="VFN58_05670"
     CDS             1898..>2046
                     /locus_tag="VFN58_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HET8917513.1"
                     /translation="MAKEKPAGEKIVCVNRQARHNYFIDETYEAGLVLVGSEVKSLRD
                     GKANL"
BASE COUNT          439 a          533 c          599 g          475 t
ORIGIN      
        1 agtggaggat gccggcgcgt tcgcggtggt gttggagggt attcctctag acttggccaa
       61 gcagatctcc gagcggctga ccatcccgac catcggcatc ggcgcgggcc cgcattgtga
      121 tggtcaggtg ctcgtgatcc atgacatgct aggtttgttc gatgatttta cgccgaagtt
      181 cgtcaagcgg tatgccgatc tccaagaggt gatgaccggg gcggtcaaaa gtttcattgc
      241 tgatgttcgc gagcgaaaat tccccggcga ggagcattcg ttcaaataac aatggggaga
      301 acggagtgat ggagtagtgg agtgatgggg atgtgcggta atccttcatt actcctacac
      361 tccggtactc caacattcca atttagctgt cctgtccatg cagatcattg agcatatctc
      421 cgagatgcaa gcttggagcg agtcgacacg gcgcgacgga cgacggatcg tttttgttcc
      481 gaccatgggt tttcttcacg acgcgcacct gagtcttgtg cgcgatggga agaagcgagg
      541 cgatcgcttg gttgtctcca ttttcgtgaa cccggcgcag ttcggtccca acgaagactt
      601 tgctgcctat ccgcgcgact tcgagcggga tcgagggttg ttggaaaggg agcacgccga
      661 tgttctgttt cacccttcgg tccgagagat ctatccggag ggctatcaga ctcacgtggg
      721 tgtggacata ctgagcgcgc tcctctgcgg cgcccatcga cctggccact tccagggagt
      781 cgccacggta gtcgcgaagc tcttcaatat cgttcggccc catgtagcga tctttggcga
      841 aaaagactac cagcagctgc agttgattcg acggctggtg cgggatttga atttcgaaat
      901 cgagatcgtc ggtcatccca tcgtccgtga gacggatggc gtggccatga gctcgcgcaa
      961 catctatctc agccctgagg aacggcaagc cgctttgtgc ctgtcccggg ctctcgttcg
     1021 agcggcatgt atggtgcaga ggggggagac cagggggcga gtcattaccg aggccgtccg
     1081 agcgggaatc gaaaaagaac cgcttgcaag agtggaatat gttaccctgt gtgattctgc
     1141 tacactggag cccgtagaag agattcagga gtcggcgttg ctggccttgg cggttcgaat
     1201 cggcaaaacc cggctcatcg acaaccagat tctcaaacca tagagggata cgtggcggac
     1261 gatacccaga agagaatgaa aatcaacgtg cgggaaattc tccagaggta ctttctagcc
     1321 gggctgttgg ttttcctacc ggtagtgatt accttatggt ttctgggctg ggtgatcggc
     1381 ctcatggacg ggttgctgga cgtgctgccg gctcgactgc atcccaactc ttacttgcca
     1441 ttcgcgattc caggcttagg cgcggctttt accatcgcat tgattctctt tcttggtgtt
     1501 cttacgcggg gtgtcgccac ccgccggttt cttgccgcct gggatcgcat cttcgttcag
     1561 atcccggtct ttcgcggtgt ctacaccgcc gtgcagaaac tggttcagac ctttttaggc
     1621 cagtcgcaga ccaacagaca ggtcgtgatg atcgaatatc cgcgtaaggg aatttatgct
     1681 gtgggttttg ccatggggcg cgcctggcat gagctggaaa agaaaaatga agctccactc
     1741 gtcaatgttt tcgtaccgac caccccgaat ccaacctcgg ggttctacct gcttgtaccc
     1801 gcgaacgaag tggcgcccct aaatatcagc atggaagaag ccctcaagct cattacctcc
     1861 ggtggattga tcacgccgga cgatagaaac aaaaaccatg gctaaggaaa agcctgccgg
     1921 ggaaaagatc gtctgcgtca accggcaagc gcggcacaat tacttcatcg atgaaaccta
     1981 tgaagccggt ctcgttctgg tcggaagcga agtaaaatcg ctccgcgacg gcaaggccaa
     2041 tctcaa
//
LOCUS       DASSWZ010000234         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_999005, whole genome shotgun sequence.
ACCESSION   DASSWZ010000234 DASSWZ010000000
VERSION     DASSWZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999005"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFN58_05675"
     CDS             complement(<1..189)
                     /locus_tag="VFN58_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917514.1"
                     /translation="MVLDVRACLKKTNLATFEQSLIKISINFTLQYGVPFGRFGGLAD
                     SRRLFSSDDDDALRASGSH"
     gene            310..1668
                     /locus_tag="VFN58_05680"
     CDS             310..1668
                     /locus_tag="VFN58_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917515.1"
                     /translation="MDKKLVLPSLIFYVLGLLALRFPLAVNAADRPLEKIRIAYTGIS
                     GAQIPVWVAYEQGFFRKYGLDVQLIFVEGGSLGVQTLVSGDVTAASVAGASVIQSNLQ
                     NSGLVMIAGFLNTMDYQFIVSKEITNPELLKGKTLAVSRFGSSSDFATRYTLERYGLT
                     PEKDVAIVQIGSQPARFAALETGKIHGAMVSIPLTAKARKMGLNALADLQMLGLEYQH
                     TGLAVTQSLIKSKPELVRNITKAFVEAIHYFKTHRKESLAVLQKYLRTDDAEALEETY
                     EAIAMTLIPRKPYPTLKGIQIMLREMGAKDPKAAATRPEQFVDLTFIEELDKSGFVDR
                     LYKATPAIASREEKPVAKPTGEEKVTLASKPMSTSQAKATAVSAKPAQPSSPTPVKMD
                     KGEEHTVKAGDTLSKLAEQYYGSAGKWEKIYEANRESIKNPNYVFIGQKIIIPAAAGS
                     GT"
     gene            complement(1839..2225)
                     /locus_tag="VFN58_05685"
     CDS             complement(1839..2225)
                     /locus_tag="VFN58_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917516.1"
                     /translation="MDLNVKLLRSPEGRNHLIIIARGLIDAEGLKLIFRHAAETIQEL
                     FNCNVLVDFENANLTLSPPNIDVLISGLGPDLRLGNIKIALVSPAEIEEFEQLRVLCI
                     SLCREDLRAAVFDNPDEAIRWLVDTM"
     gene            complement(2469..>3506)
                     /locus_tag="VFN58_05690"
     CDS             complement(2469..>3506)
                     /locus_tag="VFN58_05690"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET8917517.1"
                     /translation="QQHKAKILGRDPKTDLAVIKIDAKTSLPAVTLGNSEALRVGDWV
                     MAIGNPFGLSNTVTTGIVSAKGRTIGAGPYDDFIQTDAPINPGNSGGPLFNMAGEVVG
                     INTAIFSQSGGNIGIGFAVPVNLVKNLVPELETKGTVTRGWLGVSVQPVTPDLARSFG
                     LEKERGALVGDVVAQGPAEKAGIKRGDVIVSYDGKKIDESVTLPALVASTPVGKTVPV
                     EVMRDGKMNTINVAVGKLNDPTAAVDTKQEKSQWGLAMQNIRPEERRQMGLTGNDGVL
                     VTAVMPASPAANAGVQPGDVILQVNQAPVSSVEGVKAEVAKAKGDKPLLLLLRRADGS
                     TSFAALSPNVG"
BASE COUNT          839 a          956 c          900 g          811 t
ORIGIN      
        1 atggctaccg gatgcccgca acgcatcatc gtcgtcgctc gaaaaaagcc tgcgtgagtc
       61 agccaagccg ccgaatctac caaaaggaac tccatattgc agcgtaaaat tgatactgat
      121 tttgatcagc gattgctcaa aggtcgccaa atttgtcttc ttaaggcacg ctcgcacgtc
      181 taacaccatg tgttttcgca acaagacttc tttacaaaca agcacttaga agaccgaagc
      241 cccttccctc accgtctggc atagttctgg cgcatagatg gggaatcgaa cccctagtaa
      301 ggagaaaata tggacaaaaa actcgtgttg ccgagtctca tattctatgt cctcgggctc
      361 ctggcgttgc gctttcctct agctgtgaat gcagccgata gaccgttgga gaaaattcgg
      421 attgcctata ccggtatttc aggcgctcaa ataccagtat gggtggccta tgagcaaggt
      481 ttctttcgca aatatgggct cgatgttcag ctcatattcg tcgaaggagg atctttgggg
      541 gtccagacac tggtctctgg agacgtaacc gcagcctcgg tcgcaggggc gtctgtcatt
      601 cagagcaatt tgcaaaattc cggactggtc atgattgcgg ggtttctaaa taccatggac
      661 tatcaattta ttgtctcaaa agagatcacc aatcccgagc tgcttaaggg taagacattg
      721 gctgttagcc gattcggatc gtcttcagat ttcgccactc gctatacgct ggaaagatac
      781 gggctgacgc cggagaagga tgtcgccatt gtgcagatcg gcagtcaacc tgcccgcttc
      841 gccgcgcttg agacaggcaa aatccatggc gcgatggtat ccattccgct aactgcaaaa
      901 gcaaggaaga tggggttgaa tgccctcgcc gatttgcaaa tgctgggcct tgagtatcaa
      961 catacaggac ttgcggtcac gcaatcgctg atcaaatcca agccagagct tgtccgcaac
     1021 atcacgaaag ctttcgtaga ggcgatccat tattttaaga ctcaccgtaa ggaatcacta
     1081 gctgttttgc agaaatatct aagaaccgat gacgcagaag cgctggaaga gacctatgag
     1141 gcgatcgcaa tgactctgat tccgcggaaa ccttatccca cgctgaaagg cattcaaatc
     1201 atgttgcggg agatgggggc aaaggatccg aaggcagctg cgactagacc agagcagttt
     1261 gtggatctca cgttcattga agagctggat aaatccggat tcgttgatcg gctgtacaag
     1321 gcgactcctg cgatcgcgag cagagaagaa aagcccgttg ccaaaccgac gggagaagaa
     1381 aaagtgacac tggcgtcaaa accgatgtcg acttctcagg ccaaggcaac ggctgtttca
     1441 gccaagcctg ctcaaccctc atcccccacc cccgtcaaaa tggataaagg cgaggagcac
     1501 acggtaaaag ccggcgatac cttgagcaag ttagccgaac agtactatgg atctgcaggg
     1561 aaatgggaga aaatatatga agctaacaga gagtccatca agaatcccaa ctacgttttc
     1621 atcggccaga aaatcatcat tcctgccgcg gccggatcag gcacataaga ccatgtagcc
     1681 gtttgagcta gagtagttat ccggggcagt caaatcttga ctgccctttt ttttggcggg
     1741 agaatttcca cccattggcg agccggcaag cggccgaata ccaacgccat tgaaacatgc
     1801 tggctgcaaa tcagtgacgc tgttgtccgc ttgtaacttc acatcgtgtc gactagccag
     1861 cggatggctt cgtctgggtt atcgaagacc gccgctctca aatcctcacg gcataacgaa
     1921 atacacaaga cccttagctg ctcgaactct tcgatttccg ctggagacac gagggcaatt
     1981 ttgatgttac ccagccgcaa atccggaccg agcccactga tcagcacatc gatattgggt
     2041 gggctaagcg ttaggttagc gttttcaaaa tcaaccaaga cattgcaatt gaatagctcc
     2101 tggattgtct ctgctgcatg gcgaaaaatt agcttcagtc cctccgcgtc tatcagtccg
     2161 cgggcgatta ttatgagatg atttctccct tccggcgatc gcagtaattt aacgtttaaa
     2221 tccatcgcta agttttcacc aatcaacttg ggccactttc ccgatacttg gctcggatct
     2281 gcattccgac agctagcccc tttccgtatt cttgaaggct tcaagggaaa tcaggatgga
     2341 tcgttggcga gagccggaac tctatttgat ggtcgaggcg ggatcacaaa aaaggatagg
     2401 ctgcttagag ttgccagcct atcccctttt gttcttactg agcattgtcg aagacccagt
     2461 tactaaactc atccaacgtt gggcgacaaa gcggcgaacg aggtgctgcc gtcagcgcgc
     2521 cggagcaaaa gcagcagcgg cttgtctccc tttgctttgg cgacctcggc ctttacgccc
     2581 tcgaccgagc tcaccggagc ttggttgact tgcaagatca cgtcgcccgg ctggacgccc
     2641 gcattcgccg ccgggctggc cggcatcacg gctgtcacta gcaccccgtc gtttccggtc
     2701 agacccatct ggcgccgctc ctcggggcgg atattctgca tcgccaatcc ccactggctt
     2761 ttttcttgtt tcgtatctac cgccgcagtt gggtcgttga gtttgccgac ggccacattg
     2821 atggtgttca ttttgccatc gcgcatgacc tcgactggaa ccgtcttgcc cacgggggtg
     2881 gaagccacga gagccggcag agtgactgac tcgtcgattt tcttgccgtc atagctgacg
     2941 atcacgtcac cgcgtttgat tccggccttt tcagcggggc cttgggccac cacgtcgccg
     3001 acaagcgcgc cgcgttcttt ttctagaccg aaagagcgtg cgagatccgg cgttaccggc
     3061 tgaaccgaaa cacccaacca gccgcgcgtg accgtgcctt tggtctccag ctccggaacg
     3121 agatttttca ccagattcac cgggaccgcg aaaccaatac cgatattgcc gccgctctga
     3181 ctaaaaatcg ctgtattgat gccaacgact tcaccggcca tgttgaaaag cgggccgccg
     3241 gagttgcctg gattgatcgg cgcgtcggtt tgaatgaaat cgtcgtacgg accggcgcca
     3301 atcgttcgtc ccttggcgct aacgatcccc gtggtcactg tattactcaa gccaaaggga
     3361 ttaccgatgg ccatcaccca gtctccgacg cggagcgcct cggagttacc caaagtcaca
     3421 gccggcaaag aggtcttggc gtcgatcttg atgaccgcca agtcggtctt ggggtcccgc
     3481 cccagaattt tcgccttgtg ctgctg
//
LOCUS       DASSWZ010000235         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1197370, whole genome shotgun sequence.
ACCESSION   DASSWZ010000235 DASSWZ010000000
VERSION     DASSWZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197370"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VFN58_05695"
     CDS             <1..1106
                     /locus_tag="VFN58_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8917518.1"
                     /translation="TPSLQSSRDCSLISIRGLRKWFDSRRGRVEALRGIDLEVTEKEF
                     CVLLGPSGCGKTTTLRCVAGLERPDAGELEIAGTVANSVARGIYVPTEKRDIGMVFQS
                     YAIWPHMNVFNNVAFPLIRGRHRIPKDEIAEKVRSALKLVQLDGLEDRPATDLSGGQQ
                     QRVAMARAMVTEPKILLMDEPLSNLDARLREQMRVELKKITKAVGVTTLYVTHDQAEA
                     LSLGDKVCVMHDGEILQMAPPHEVYARPTSLFVAQFVGEMNFVKGHVTGPSQVECPLG
                     SLSLTVPTGISVSSPVTLAIRPEHVKVNSNDGSTSGLSGNITSKNYLGDAALLEVEVN
                     GVTLLAKLAGDSELAVGQPAAVDLPSHRWHVFP"
     gene            1247..>2044
                     /locus_tag="VFN58_05700"
     CDS             1247..>2044
                     /locus_tag="VFN58_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET8917519.1"
                     /translation="MTTTQFSRKPVENLLSARSVAIVGASTKGRWPSGIYRNLKAAKF
                     PGNIYLINPNYTEIFDDKCYPNLAALPTVPEHLLMLIPTRAVLGTIEEAAKLGTRAAT
                     IYSAGFGEGDDPKGKERAQAMKELCDRTGFVVCGPNCMGSFSLPEGLWSFPTPVPLLK
                     KGPVGLIFQSGGSLGNWVKGASERGIGFTYAVSSGNEVSLDLVDYLSFLVDEPDTKVI
                     TLMVEGIRRPQEFMAVAEAALKKNKPILVVKLGRSEMGKRQAISHTGS"
BASE COUNT          450 a          543 c          601 g          450 t
ORIGIN      
        1 gtactccatc actccaatcc tcacgggatt gttccttgat cagcattcgc ggactcagaa
       61 agtggttcga cagccggcgc ggccgcgtgg aagcactgcg cggtatcgac ctggaagtga
      121 ccgagaaaga gttctgcgtc ctactcggcc cgagcggctg cggcaagact acaaccttgc
      181 gctgtgttgc cggtcttgaa aggcccgatg ccggggagct ggaaatcgcc ggcacggtgg
      241 cgaactccgt ggctcgggga atctacgtcc ccacggagaa gcgcgatatc ggcatggtgt
      301 ttcagtccta cgcaatctgg ccgcacatga acgtgtttaa taatgtcgcg ttccccttga
      361 tcagagggcg ccataggatc cccaaggacg aaatcgctga gaaagttcgc tccgccctga
      421 aacttgtgca attggacggg ctcgaggatc ggccggcgac tgatctgagc ggcgggcagc
      481 agcaaagagt cgcgatggcg cgggcgatgg tgacggagcc aaaaattctt ttgatggacg
      541 aacctctgag caatctcgac gcccggctgc gcgagcagat gcgtgtggag ctgaaaaaaa
      601 tcaccaaggc ggtcggcgtg acgactctct acgtgactca cgatcaggcg gaggctttga
      661 gtctcggcga taaggtctgc gtcatgcatg atggcgagat tcttcagatg gcaccgcctc
      721 acgaagtata cgctcgtccg actagcttgt ttgtggcgca attcgtaggg gaaatgaatt
      781 ttgtcaaagg tcacgtcacc gggccatcgc aggtggaatg tcctctgggg agtctttcgc
      841 ttaccgtgcc aacggggatt tcagtctcaa gtcccgtgac attggcgatt cggccggaac
      901 atgtcaaggt caactcaaat gatggttcga cttcaggctt gtcgggaaac atcaccagca
      961 agaactatct cggtgacgct gctctgcttg aggtcgaagt taacggcgta accttgcttg
     1021 caaaactcgc cggagactcg gagctcgctg ttggacaacc agccgcggta gacttgcctt
     1081 cccatcgctg gcatgttttc ccttgaattc agttgccggt ggtcagggac caggatcgca
     1141 gatcgagatt taatttcccg acaactaagg tctggcaact attccacctt gcagtcactc
     1201 ggatactgtg acatagggga aaaaactcca tgaaaaagga attgatatga caacaaccca
     1261 attctcgcgc aagccggttg agaatcttct ctcggcgcgt tccgttgcca tcgtcggcgc
     1321 gtcaaccaag ggccgctggc cttcgggtat ctatcgcaat ctcaaggcgg cgaagttccc
     1381 cggcaatatc tacctgatta atccgaatta cacggagatt tttgacgata aatgctatcc
     1441 gaatcttgct gctttgccga cagtgcccga gcatctgctg atgctcattc cgacgcgggc
     1501 tgttctcggc acgatagaag aagccgccaa gctaggtacc agggccgcga ccatctacag
     1561 cgccggtttc ggcgaaggcg acgatcccaa gggaaaagaa cgtgcgcaag ccatgaagga
     1621 gttgtgtgat cgcaccggtt ttgtcgtctg cggcccgaac tgcatggggt cgttctcgct
     1681 gcccgaagga ctgtggagtt ttccgactcc cgtgccgctt ttgaagaagg gaccggttgg
     1741 tttgatcttt cagagtggcg gttcgctggg caactgggta aagggagcgt cggagcgggg
     1801 cattggattt acctacgccg tgtctagtgg aaacgaagtc agtctcgact tggtcgatta
     1861 tttgtcgttc ctcgtcgatg agcccgacac caaggtcatc actctaatgg tcgaaggcat
     1921 ccgccggccc caggagttca tggccgtggc tgaggcagcg ctaaaaaaga ataagccaat
     1981 tctcgtggtc aagctgggcc gttctgaaat ggggaaacgc caggcgattt cccacaccgg
     2041 ctcg
//
LOCUS       DASSWZ010000236         8570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1225654, whole genome shotgun sequence.
ACCESSION   DASSWZ010000236 DASSWZ010000000
VERSION     DASSWZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225654"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /gene="rpmG"
                     /locus_tag="VFN58_05705"
     CDS             <1..99
                     /gene="rpmG"
                     /locus_tag="VFN58_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET8917520.1"
                     /translation="TTKNRKKTTEKLSLKKFCSSCRTHTLHREVKV"
     gene            111..188
                     /locus_tag="VFN58_05710"
     tRNA            111..188
                     /locus_tag="VFN58_05710"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:146..148,aa:Trp,seq:cca)
     gene            210..425
                     /gene="secE"
                     /locus_tag="VFN58_05715"
     CDS             210..425
                     /gene="secE"
                     /locus_tag="VFN58_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET8917521.1"
                     /translation="MGDWIEKVQDGVRRLTEFFQESWQELKKVHWPSRKETYAATLVV
                     IIVVVLISVYLALVDLGLTKAIQAIVD"
     gene            443..973
                     /gene="nusG"
                     /locus_tag="VFN58_05720"
     CDS             443..973
                     /gene="nusG"
                     /locus_tag="VFN58_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET8917522.1"
                     /translation="MAKQWYIVHTFSGYEQKAKAALEERIKALGKQDYFSEILVPVEK
                     VVELVKGKKKTSSRKFFPGYILVQMELNDDTWHVVKETPKVTGFVGGSTNPPPVSDEE
                     VRTITQQMEEGAIKPKPRVLFSVGENVKVVDGPFADFNGVVEEVKPDKGKLRVLISIF
                     GRATPVELEFVQVERN"
     gene            992..1417
                     /gene="rplK"
                     /locus_tag="VFN58_05725"
     CDS             992..1417
                     /gene="rplK"
                     /locus_tag="VFN58_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET8917523.1"
                     /translation="MAKKVIGEIKLQIPAGQANPSPPVGPALGQRGVNIMEFCKSFNA
                     ATQSQQGMIIPVIITVYADRSFTFVTKTPPASVLLKKAAGVEKGSSDPGKMKVGKVTR
                     AQVREIAELKLKDLTAKDLAAAEKSVEGTAQSLGLEIEG"
     gene            1442..2146
                     /gene="rplA"
                     /locus_tag="VFN58_05730"
     CDS             1442..2146
                     /gene="rplA"
                     /locus_tag="VFN58_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET8917524.1"
                     /translation="MSGNGKSYRAALAKLDRSQRYLLEDGLRLVKETARAKFDETVEM
                     AVRLGVDPRQADQNVRGTVSLPHGMGKTVRVLAFAKGEKEKEAQEAGADFVGSEDLIK
                     KISEGWFDFDKTVATPDMMAAVGRIGKVLGPRGLMPNPRTGTVTADIGKAVKEIKAGK
                     LEFRVDKAGIVHVPVGKASFNPEKLIDNAKAVLTSILRAKPASAKGNYVRGVTVSTTM
                     GPGIKIDLSQVRAMAA"
     gene            2208..2750
                     /gene="rplJ"
                     /locus_tag="VFN58_05735"
     CDS             2208..2750
                     /gene="rplJ"
                     /locus_tag="VFN58_05735"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET8917525.1"
                     /translation="MAELEVVRASHGEKAEAVAFVHEKFKTAKMAIVTEYRGLSVAQM
                     TRLRRAIRDASGEYQVIKNTLVRRALKDTAYGSLDRLLDGPNGWVFAYDDPVMLSKAL
                     VKFADDNDKLAIKGGVFEGQFMEPAKVKVLAQMPSKPELQAKLLALIQAPATQLVRLV
                     QEPGARVVRLLETLRKGKSE"
     gene            2800..3177
                     /gene="rplL"
                     /locus_tag="VFN58_05740"
     CDS             2800..3177
                     /gene="rplL"
                     /locus_tag="VFN58_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET8917526.1"
                     /translation="MPEVSKEQVKDFIKNMTLLDAAALVKELEEELGVSAAAPVAVAA
                     AAGPAAAAAEEKTEFNVILAEAGEKKIQVIKVVRELTGLGLKEAKDLVDGAPKAVKEG
                     VAKAEAESVKKKLEEQGAKVEIK"
     gene            3246..7349
                     /gene="rpoB"
                     /locus_tag="VFN58_05745"
     CDS             3246..7349
                     /gene="rpoB"
                     /locus_tag="VFN58_05745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET8917527.1"
                     /translation="MAFQVAHNLRFRRSFGRIKKIIDLPYLIEIQKNSYDQFLQREVA
                     PAQRQNFGLQEVFKSVFPIKDFNETASLEFVSYALGEPKYDVEECHQRGMTFAAPLKV
                     TVQLVLWDVDAQTGARSIKNVKEQEVYFGEIPLMTKNGTFMVNGTERVIVSQLHRSPG
                     VFFEHDKGKTHASGKLLYSARVIPYRGSWVDLEFDPRDILYVRIDRRRKFHATVLLRA
                     LGMTTEDLLNYYYRSDTIVFDGRKPALLFNPQHLLGAKVGSDIRDPKTNDLIVKEGKK
                     FTRPVLRQMESARIKQVPIDWQEIVGRIVSHDLIDPQTKEVVVECNHEITQEKLDLIR
                     ERGIHQIEVLFIDESNVGPYLRNTLLQDRIRSPQDAILEIYRRLRPGDPPTVESATNF
                     FNNLFFNPDRYDLSKVGRLKLNHRLRLNVPLEQGTLRKEDILEVVRYLMELKNGNGSV
                     DDIDHLGNRRVRAVGELVENHFRVGLVRMERAIKEKMSLQDIETLMPQELINYKPVSA
                     ALKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRV
                     CPIETPEGPNIGLIASLTTYARVNEFGFIETPYREVENGRVTDRIRYLSALEEEDHVI
                     AQANAPIDGRGVFTADLISARKGGEFVMARPEEVNFMDVSPNQLVSVAASLIPFLEND
                     DANRALMGSNMQRQAVPLLRTEAPFVGTGMEKTVARDSGVTIVARRDGVVENVDSTRI
                     VVKADKPSGARDTGVDIYNLVKYQRSNQNTCVNQRPIIVVGDQVKAGDVIADGPSTEM
                     GELALGRNVVVALMPWGGYNFEDSILISERVVKEDIYTSIHIEEFECVSRDTKLGPEE
                     ITRDIPNVGDEALTDLDESGIIRIGAEVKPGDILVGKITPKGETQLSPEEKLLRAIFG
                     EKAGEVRDTSLRVPPGVEGTIINVRIFSRKGLGKDERSKAIEDEEITRLRKDQHDEVR
                     ILQEGALKRLKKLLVGRRLAVRLSDDGRKILLNKGKEITEEDLDQLPMSYWGDIRVDN
                     ESVEGELNRIVESTTEQVSLIKDLFEDKIDKLRGGDELPPGVIKMVKVFVAIKRKLQV
                     GDKMAGRHGNKGVISRILPEEDMPYLEDGTPVDVVLNPLGVPSRMNVGQILEAHLGWA
                     ARSLGQQIDELLRSHRDEIDALRRKLKDYLNTKEASELIEGAKEADVFKLAEKYRGGV
                     HLASPVFDGAAEEETFNLLRKAGLPVTGQVTLYDGRTGEPFDGKVTVGVMYIMKLHHL
                     VDDKIHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAAYTLQEMLTVKSD
                     DVIGRTRMYEAIVKGDNLLEPGLPESFNVMVKELQSLGLDVDLVEEK"
     gene            7396..>8570
                     /locus_tag="VFN58_05750"
     CDS             7396..>8570
                     /locus_tag="VFN58_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET8917528.1"
                     /translation="MEDLFNLFEKPKNPLSFNAIKVSIASPEKIRSWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPTKDYECNCGKYKRMRHRGVVCEKCGVEVIQSKVRRERM
                     GHIELATPVVHIWFLKSLPSRIGALLDMSLKEIEKVLYFESYIVIDPGETPLNYKDLL
                     TESQYRKKVEEYGGRFIAEMGAEAIRKLLKSVDVAKLSEDLRQEMRDANSEVRRKKMA
                     KRLKVINAFKTSGNRPEWMVLEVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLMELSAPDMIIRNEKRMLQEAVDALFDNGRRGRAITGQNRRPLKSLSDMLKGK
                     TGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKKMALELFKPFIYNKLEERGLVT
                     "
BASE COUNT         2199 a         2035 c         2462 g         1874 t
ORIGIN      
        1 acgacgaaaa acagaaagaa gaccacggaa aaattatccc ttaagaaatt ctgttcttct
       61 tgccggaccc ataccctgca tcgcgaggta aaagtctaaa agagatacag gggtcagtag
      121 ctccaattgg ctagagcgcc ggactccaaa tccggttgtt gcaggttcga gtcctgcctg
      181 acccgccatc tcatcgcgtt gggtatttca tgggagattg gatcgagaaa gtccaggacg
      241 gtgttcggcg cttaactgaa ttcttccaag agtcctggca ggaactaaag aaagtccatt
      301 ggccatcacg taaagagact tacgccgcga cgttggttgt cataattgtc gtcgtattga
      361 tttccgtgta cctggcactg gtggatttgg gccttaccaa agcgattcag gcgattgtcg
      421 actagcctct taggtttccc gtatggcgaa acaatggtac atcgttcaca ccttttcggg
      481 atatgagcag aaggccaagg ccgctctaga ggaaagaatc aaggcgctcg gcaaacagga
      541 ctactttagt gagatattag taccggtaga gaaggtcgtc gagctcgtca aagggaagaa
      601 gaagacttct tcaaggaagt ttttcccggg ctatatattg gttcaaatgg agctcaatga
      661 cgatacttgg catgtcgtta aggaaacgcc taaggttacc ggtttcgtcg gcggaagcac
      721 aaatccaccg ccggtgagtg atgaggaggt tcggaccatt acccaacaga tggaggaagg
      781 ggcgatcaaa ccaaaacctc gggttctctt ttcggttggg gagaatgtta aagtggtgga
      841 tggtccgttt gcggatttca atggcgtggt ggaagaagtc aagccggaca aaggcaagct
      901 cagagtactg attagcattt tcggaagggc cacgccggtg gagttggaat ttgttcaggt
      961 ggagcggaac taaacagaag ggtcaagcac tatggcgaag aaggttatcg gcgagatcaa
     1021 acttcagata ccggcgggcc aggcgaatcc aagcccgcct gttggcccgg ctttgggtca
     1081 gcggggtgtc aatatcatgg agttttgcaa atccttcaac gccgcaaccc aatctcagca
     1141 gggaatgatc atccctgtaa tcatcaccgt atacgcggat cgctccttca cgttcgtgac
     1201 gaagacccct cctgcgtcag ttcttttaaa gaaggcggct ggagttgaga aaggctcatc
     1261 cgatcctggg aagatgaagg ttggtaaggt tacacgagct caggtcaggg aaatcgcgga
     1321 acttaagctc aaggacctga cggcaaagga tctggcagcc gcggagaagt ccgtggaggg
     1381 taccgcccaa agcctcggtc tggaaatcga gggctgagac aaacggcctg aaggaaaaaa
     1441 catgagcggc aatggaaaat cttatcgagc tgcattggct aaactagatc gtagccagcg
     1501 gtatcttttg gaagacggcc ttcgactcgt gaaggaaacc gcccgcgcca agtttgatga
     1561 aaccgttgag atggctgtgc gcttgggtgt ggatccacgg caagcagacc aaaacgtccg
     1621 gggtacggtg agtctcccgc acggtatggg gaaaacggtt cgggttcttg ccttcgccaa
     1681 aggtgagaag gaaaaagagg cgcaagaggc tggcgcagac tttgtcggta gtgaagatct
     1741 tatcaagaaa ataagtgaag gttggttcga cttcgacaag acagtagcga ctcccgatat
     1801 gatggcggcc gtgggacgaa tcggtaaggt cctgggccca agaggcctca tgccgaaccc
     1861 caggacgggg acggttaccg cggatatcgg taaggcagta aaagagatca aagcaggaaa
     1921 actagaattt cgcgtcgaca aagcagggat tgtgcatgtg ccggttggaa aggcttcttt
     1981 caatccggaa aaactgatcg acaacgcaaa ggccgtctta acgtccattc taagagccaa
     2041 gcccgcgtcc gctaagggaa actacgttcg cggcgtgacg gtctccacga ctatgggacc
     2101 tggaattaaa atcgatcttt cgcaagtgcg tgccatggct gcgtaagatc gtaatcttta
     2161 gggcattagt aagtatcaat tttctgaatc gatatcagga gaagagtatg gcggagctgg
     2221 aagtcgtacg cgcgagtcac ggcgagaagg ccgaagcggt ggcgtttgtt catgaaaaat
     2281 tcaaaaccgc gaagatggcc atcgtcacgg aatatcgcgg cctcagcgtg gctcagatga
     2341 cccgcctgag acgggcaatt cgtgacgctt cgggggaata tcaggtcatc aagaataccc
     2401 tcgtgcgccg ggcgcttaag gacactgctt atggttcttt agaccgcctg cttgacggcc
     2461 cgaacggttg ggtctttgcc tatgatgacc cagtcatgct ctccaaagcc ttggtcaaat
     2521 ttgccgacga caacgacaaa ttggccatca agggcggtgt ctttgagggg cagttcatgg
     2581 agccggccaa ggtgaaggtt ttggcccaga tgccgagcaa gccagaactt caagcgaaat
     2641 tactggcgct cattcaggct ccggcgactc aactagttcg acttgtgcaa gaaccaggag
     2701 cgcgggttgt cagattgttg gaaacgctac gcaagggaaa atcagagtga cgctatccga
     2761 gatgttgttt tggaacaaaa ctaaaagggg gaagcttgta tgccagaggt atccaaagaa
     2821 caagtgaagg actttatcaa gaacatgacg ctgctggatg cggcggctct tgttaaggag
     2881 cttgaagaag agttgggcgt gagcgcagcg gcgcccgtag ccgttgcagc agctgccggt
     2941 cccgcggctg cggcagcgga agaaaagact gagtttaacg ttattctcgc cgaagcgggt
     3001 gaaaagaaga tccaggtcat caaggtcgtt cgcgagctta ccggtttagg cttgaaggaa
     3061 gctaaggatt tagtggacgg ggcgcccaag gctgtcaaag agggtgtagc caaggccgaa
     3121 gcggaaagcg tcaagaagaa actcgaggag cagggagcga aagtcgagat caagtagttt
     3181 tccgctttca gggtttttct ggtcgatgac ggatctactt ccgcaagttg gatagaggag
     3241 caagcatggc atttcaagtg gctcataatt tacgttttcg tcgcagcttc ggtagaatca
     3301 agaagataat tgatttacct tacctaattg aaatacagaa gaattcctac gatcagttcc
     3361 tgcagcggga agttgccccg gcgcagcgcc agaatttcgg cctgcaggag gtctttaaat
     3421 cagtttttcc gattaaagac ttcaatgaga cggcgtcatt ggagttcgtg agttacgcgc
     3481 tcggcgagcc caaatacgac gtagaggaat gccatcaacg agggatgact ttcgctgcgc
     3541 ctttaaaagt gaccgtgcaa cttgtcctgt gggacgtcga tgcgcagacc ggtgcccgca
     3601 gcatcaagaa cgtgaaagaa caggaagtct actttggcga gattccgctg atgaccaaga
     3661 acggcacgtt catggtcaac ggaaccgaac gagttatcgt cagccagctc caccgttccc
     3721 cgggagtttt cttcgaacac gataagggaa agactcatgc tagcggcaag ttgctctatt
     3781 ctgcccgagt gatcccctat cgtggctcct gggtcgatct cgagtttgac ccccgggaca
     3841 ttctttatgt gcgcatcgac cgccggcgca agtttcatgc aacggttttg ttgcgtgcat
     3901 tgggaatgac gacagaggat ctgctgaact actattatcg gagcgacact atcgtttttg
     3961 atggtcgcaa gcccgcgctg ttgtttaacc cgcagcacct cctcggggcc aaggtcggct
     4021 cggacatacg agatccaaaa accaatgatc tgatcgtcaa ggaaggtaag aaatttaccc
     4081 ggccggttct ccgtcagatg gagagtgccc ggatcaaaca ggtgccgatc gattggcaag
     4141 aaatcgttgg ccggattgtc tcccacgatc ttattgatcc ccagacgaag gaagtggtcg
     4201 tggaatgcaa ccatgagatc acacaggaga aactggatct catccgcgaa cggggaattc
     4261 atcagatcga ggttctgttc atcgacgaat ccaatgtggg gccctatctt cgcaataccc
     4321 tactgcaaga tcgcatccgt tccccccagg atgctatcct agagatctac cggcggctcc
     4381 ggccgggcga tccacccaca gtcgaatccg cgactaactt ctttaacaat cttttcttta
     4441 atccggatcg ctacgatctc tccaaagtgg gtcggctcaa attaaatcac cggcttcgac
     4501 tgaatgtgcc tttagagcaa gggaccctgc ggaaagaaga tatcctcgag gtagtccgtt
     4561 atcttatgga gcttaagaac gggaacggtt ctgtggatga tattgaccac ctcggcaatc
     4621 gccgtgtgcg ggccgtcggc gagctcgtcg aaaaccattt ccgcgttggg ttagtaagga
     4681 tggagcgggc gattaaagag aagatgagtc tgcaggatat cgagaccctg atgcctcaag
     4741 aactcatcaa ttacaagccg gtttcggccg cgctcaaaga gtttttcgga tcgagccagc
     4801 tgtcgcaatt catggatcaa accaatcctt tgtctgaaat cacccataag cggcgtcttt
     4861 cagccctcgg gccaggtgga ttgacgcggg aacgtgccgg gttcgaagtg cgcgacgtcc
     4921 atccgaccca ctacggtcgt gtttgtccga ttgagacccc ggaagggccg aacataggct
     4981 taatcgcatc gctgacaacc tacgctagag tcaacgagtt tgggtttatc gagactccgt
     5041 atcgcgaggt ggagaacggt cgcgtcaccg atcggattcg ctatttgtca gctttagaag
     5101 aagaagatca cgtcatcgct caggctaacg cgcccatcga tggccgaggt gtattcacgg
     5161 ctgatctgat atcggcgcgc aaaggtggtg agttcgtcat ggcgcgtccc gaagaggtta
     5221 atttcatgga cgtctcgccc aatcagctgg ttagtgtggc cgcctcgttg attcccttcc
     5281 tcgaaaatga cgatgccaat cgagccctca tgggttcgaa catgcaacgg caggcggttc
     5341 cgctgctgcg gaccgaagct ccttttgtcg gtaccgggat ggaaaaaacg gttgcgcggg
     5401 attccggcgt taccatcgtg gcccggcgtg acggtgtagt ggaaaacgtt gattcgaccc
     5461 ggattgttgt taaggcggac aagccttccg gcgcgcggga cacgggtgtc gatatctaca
     5521 atctagtcaa gtatcaacgc tccaatcaaa atacttgtgt gaatcagcga ccgattatcg
     5581 tcgttggcga tcaggtaaag gcaggtgacg taattgccga cggtccttcg actgaaatgg
     5641 gcgagttggc gttgggacgc aatgtggttg tggccctaat gccttggggc gggtacaact
     5701 ttgaggactc gatcctgatc agcgaacggg tcgtgaagga agatatctat acgtcgatcc
     5761 acatcgaaga gttcgagtgt gtttcccgcg acaccaagct gggtccagaa gaaattaccc
     5821 gcgacattcc taacgtcggc gatgaggctc tcacggatct tgatgagagc ggcattatcc
     5881 gaatcggtgc agaagtaaaa ccgggcgata ttttggtcgg aaagattact ccgaaaggcg
     5941 aaactcaatt gtcgcccgaa gaaaagctgt tgcgcgccat cttcggcgag aaggctggcg
     6001 aggtaaggga tacctcattg agagttcccc cgggcgtaga ggggaccatt atcaatgtca
     6061 ggatcttctc gcgtaagggg ctgggtaaag atgagcgaag taaggcgatc gaagacgaag
     6121 aaatcaccag gcttagaaag gatcagcatg acgaagtccg gatcctgcaa gaaggagccc
     6181 taaagcggct caagaaacta cttgtcggac gacgcttagc ggttcggtta agtgacgatg
     6241 gtcgcaagat tcttctcaac aaagggaaag aaattacgga ggaagatctc gatcagctgc
     6301 cgatgtccta ttggggcgat atccgagtcg ataatgaatc ggttgaaggc gagctcaatc
     6361 gcatcgtcga aagcaccacg gagcaagtga gtctgatcaa ggatcttttt gaagataaaa
     6421 tcgataagtt gagaggtggg gatgaactcc cacccggagt catcaagatg gtcaaggttt
     6481 tcgtcgccat caaacgaaag ctgcaggttg gcgataaaat ggccgggcgc cacggaaaca
     6541 aaggagtgat ctcgcgcatt ttaccggaag aagacatgcc ctatctcgaa gacggaaccc
     6601 cggtcgacgt agtactcaac ccattagggg tgccttctcg aatgaacgtc gggcagattc
     6661 ttgaggccca tctgggttgg gcggcgagaa gccttggaca gcagattgac gagttgctac
     6721 gcagccatcg tgacgagatc gacgcgctca ggcgaaagct caaggactat ttgaatacta
     6781 aggaggcgag cgaactcatc gagggagcca aggaggccga tgttttcaaa cttgccgaga
     6841 agtaccgcgg tggcgtacac ctggcgtccc cggtgttcga tggggctgcc gaggaagaga
     6901 ctttcaatct gctcagaaaa gcgggattgc cagtgaccgg tcaagtcacc ctgtatgacg
     6961 gccgcaccgg tgaaccgttc gacggcaaag tgaccgtagg tgtcatgtac atcatgaaac
     7021 ttcatcacct cgtggacgac aaaattcatg ctcggtccac cggaccttac tccctcgtca
     7081 cccagcaacc gctaggtggg aaagcccaat ttggcgggca gcggctcggt gaaatggagg
     7141 tatgggcact ggaggcctat ggcgctgcat acacgcttca ggaaatgctg acggtcaagt
     7201 cggacgacgt aattggcagg acacgaatgt acgaggcaat tgtcaaggga gacaatttgc
     7261 ttgagcccgg actccctgaa tcgtttaacg tcatggtgaa agagttacag agcttaggct
     7321 tggacgtgga cttggtcgaa gaaaaatagg ttaaccccag cgatgctcaa tcgctaaatt
     7381 cggaggtggc cgtttatgga agatctattc aatttgtttg aaaaaccgaa aaatcccctc
     7441 agtttcaatg caatcaaagt ctccatcgcc tcgcccgaga agatccgttc gtggtctcac
     7501 ggcgaggtta agaaacccga aaccatcaat tataggactt tcaagccgga acgggatggt
     7561 cttttctgtg ccaaaatttt tggcccaacc aaagattacg agtgcaactg cggtaaatac
     7621 aagaggatgc ggcatcgtgg cgtcgtctgt gagaagtgcg gagtcgaagt tattcagtcc
     7681 aaagtccgcc gcgaacggat gggccatatc gagttggcga cgccagtcgt acatatttgg
     7741 tttctcaaga gcctccccag ccggatcggc gcgctgctgg atatgtcgct caaagaaatc
     7801 gagaaggttc tttactttga atcatacatt gtcatcgatc cgggcgagac accgttaaac
     7861 tataaggatc tcttgaccga gagccagtat cggaaaaagg tcgaggagta cgggggaaga
     7921 tttatcgccg agatgggcgc ggaggccatt cgtaagttgc tcaagtcagt cgacgtggcg
     7981 aaactttcag aggatttgcg ccaggagatg cgcgacgcca attccgaagt gcgccgcaaa
     8041 aaaatggcca aacgattgaa ggtcatcaat gcctttaaga cctcaggtaa ccgacccgag
     8101 tggatggttt tagaggttat tccggtcata ccgccagatc tccggccgct cgtgccgctt
     8161 gatggcggac ggtttgcgac ttcagacctc aacgatcttt atcgccgagt catcaaccgg
     8221 aacaaccgtt taaaacggct catggaactc agcgcgccgg atatgattat tcgtaacgag
     8281 aagcgcatgc tccaagaagc cgttgacgct ttgttcgaca acggccgacg gggccgtgcg
     8341 atcactgggc aaaaccgtcg cccgctgaag tctttgagcg acatgctcaa aggaaaaacc
     8401 ggtcgcttcc gccaaaacct gctgggaaaa agagtcgact actctggccg ttcggtcatc
     8461 gtcgtcggtc ctgaactccg gttgcatcag tgcgggttgc cgaaaaaaat ggcgctcgag
     8521 ctgttcaagc cctttattta taacaaactc gaagaacgtg gacttgttac
//
LOCUS       DASSWZ010000237         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1310436, whole genome shotgun sequence.
ACCESSION   DASSWZ010000237 DASSWZ010000000
VERSION     DASSWZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310436"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..322
                     /locus_tag="VFN58_05755"
     CDS             122..322
                     /locus_tag="VFN58_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917529.1"
                     /translation="MPSDETRRVLRTFGVAVTTYEDAVEAGAPADEIDKAETEFRATL
                     SEVTALIERLRTKRNTRSQQKP"
     gene            complement(909..1514)
                     /locus_tag="VFN58_05760"
     CDS             complement(909..1514)
                     /locus_tag="VFN58_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917530.1"
                     /translation="MILDLTTIPIGKAIGLMRHLAGVACIVLLASLAQAQTSKETPPK
                     LSAELERVRSALIKYQDPIKAVHDGYFSTVGCVRYPTGGMGIHFLNPQLIGPVPDPMR
                     PTILLYEPVGDKLRLVGAEWFIPLATGVKERPQLFGQPFDGPMEGHEPLMPATLRHYD
                     LHVWLFKSNPAGLFNMVNPNVNCPKTAYTFLEHPPKTLPQQ"
BASE COUNT          533 a          564 c          543 g          483 t
ORIGIN      
        1 gagctcaagg agatgatcgg gggctactac caagccagag gattgactga gaacggcttt
       61 gttccagtga aaaaactgca ggagctggag ctttcaaacc tccggctgaa ggagtgaaat
      121 catgccaagc gacgaaacac gccgggtact gaggacgttt ggcgttgccg tgacaaccta
      181 cgaggacgcc gtcgaagcgg gggccccggc cgacgagatc gacaaagctg agaccgagtt
      241 ccgcgccact ctcagcgagg tgacggcttt gatcgaacgg ttgcgcacga aaagaaacac
      301 gcgttctcag caaaaaccgt aaaacgaaaa tttgatttcg atcggttcag cttagagatc
      361 ttcacgattt cctgagcgag aacggatcag cggttgacga gttcacatag tgccagaagc
      421 aggatcgcga tatgtgtcgg gataaaccca aacgtcagca cgctcatggt acgagttgcc
      481 aagtcttctg cggaactgag acctgggaag aattttagga caccgaacca gaaaaagaca
      541 acgcccagac tgacacgcaa aaacataatg ccatagtgcg ccatccaatt tggtaagggg
      601 cacatcaata gactcaaaca acttatagag acttgccacg ttgctttcct tgcgttaatg
      661 caatactcac ccgcccgtgg atgatggaat tttcgattgc gacaaagccg cccaacgctc
      721 cgcttgaggg cgcgccgctt tttggcggtc cttctcgaag cggttgttag gcgatccaaa
      781 gtcaagcaat gacccccatc tttatttgac tcccatcttt attacgcgat tgcaaagacc
      841 cgtccatatt tcttctcgcc agaaaactct gctcccactc cgttcgtcga tcgaataggt
      901 gtcgcgtctc actgctgcgg cagtgtcttc ggtgggtgct ccaggaaagt ataggcggtc
      961 ttcggacagt tgacattggg gttgaccata ttaaacagcc ctgcgggatt ggacttgaat
     1021 agccacacat gcaggtcata gtggcgaagg gttgctggca tcaggggctc atgcccttcc
     1081 ataggaccgt caaagggctg accaaagagc tgcggacgct ccttcacgcc cgttgccagc
     1141 ggaatgaacc attcggcacc gaccaagcgg agtttgtcgc ccacgggctc gtataggagg
     1201 atcgtcggac gcatcggatc tgggacgggc ccgatgagct gtgggttcag gaagtggata
     1261 cccatccctc ctgtgggata cctgacgcat cccaccgtcg aaaagtagcc gtcgtggaca
     1321 gccttgatag gatcctgata cttgatcagc gcagagcgca cgcgttcgag ttctgctgac
     1381 aatttcgggg gagtctcttt agatgtttga gcttgggcaa gggatgcgag caacacgatg
     1441 caagcaacgc ccgcgagatg tctcatcaga ccgatggctt tgcctatcgg tatagttgtc
     1501 aaatctagga tcatgaaaat ctccttggaa ggaatccccc tcgaaactcc gccttacacc
     1561 gtcaagctat ttgctcgaag atgtggtcca attctcctat catcaaatac cttctcttct
     1621 caatcgatgc tgtcgctcga ctaagtagtg catgacaatc ctcttttcgc gaaagcctaa
     1681 ctattcagtg agccgccgta ggaaacgaca aacgccggtc agcctatctc ctgggacgat
     1741 tccatcactc aaatccgatc cggctgcaat gtcccaccag ccatcgcaac ttgtcaacgt
     1801 tctgccgggg tcggcgggcc ttctccctaa agaggaggtt tccccccgca cattcacccg
     1861 agatacgagc cgagtcgctt tctgcaactg atctatctct actggcgaat cagaattagg
     1921 cgagacagaa ggtattactt tcaggtgatg gcaggtgaaa tatctccctt tcacgttgtg
     1981 taacaattgc ggacaactgt tttgtaggag atctctaaac ggaacgatct tgtcaaaccc
     2041 cgattggggc aatgtttgca aattattgct tgccagtcag aaaaaaaacc tccgcatttc
     2101 cttttaaacg cacgatctca ctc
//
LOCUS       DASSWZ010000238         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1395411, whole genome shotgun sequence.
ACCESSION   DASSWZ010000238 DASSWZ010000000
VERSION     DASSWZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395411"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..983
                     /locus_tag="VFN58_05765"
     CDS             237..983
                     /locus_tag="VFN58_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8917531.1"
                     /translation="MIKVVDLHKTFGGQAVLKGINLELETGKVTTIIGGSGSGKTVLL
                     KHLNALLLPNHGSVLVDGKDITKLGERELNEVRQKFGVLFQGAALLDSMTLYDNVAFP
                     LREKTKMSENEIRKRVEERLAQVGLAGMGYKYPAEVSGGMKKRAGLARALAMEPEIVL
                     FDEPTTGLDPLLGKSIHRLIGKMHATFGFTGVIVSHDIPEVFKISDRVAMLANGVIAE
                     AGSTQEFVASKNPVVRQFLQGDAEGPLTVL"
     gene            995..1471
                     /locus_tag="VFN58_05770"
     CDS             995..1471
                     /locus_tag="VFN58_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HET8917532.1"
                     /translation="MTQTRMEIIVGLFVLVGMACLGYLAIKLGKLEVLGNSGYPVYAD
                     FSSVAGLKMGDPVEIAGVKIGRVEAMGLVDDRARLQLRLQDGVKIQEDAIASVRARGL
                     IGDKFVLITPGASDKIIPPGGRLRETESPPDIADLIGKIVGGDLTSKSEKKEESQK"
     gene            1646..2581
                     /locus_tag="VFN58_05775"
     CDS             1646..2581
                     /locus_tag="VFN58_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917533.1"
                     /translation="MATWIDWHSHHTAPEAVETFAEFTGKLPKIDDYDSIDFADRVQQ
                     MDEVGIDLQLICQGAGLYADQLPADQALAIVRQSNDLLAQRIAPHRQRLMGVTALSLK
                     NIEASVEELERTSTRGFRAALLYPTVDGEMVVDLPVMDPLYAKIAELEWPIFLHGAGL
                     AKDPTLKRLEDGGAGVAFAVLSDAEISECALRMIAGGVFDRYPGLRIVIRSSGGGLPL
                     LLHRFFWKHKGPRGEQRYSEIFLEHFLIDCASSDARTLSFLIDTMGEEHVVFGSDYCG
                     GLGPLKKALASVENQTNPEYLKSFTEKNSRRLLHI"
BASE COUNT          586 a          696 c          744 g          577 t
ORIGIN      
        1 caggcgacca ccgaatcggt cgtcttgtgc ttcgtcctga tcctggtttg ggactacttc
       61 atgacttcgg tcatgctcta acaggctgct gaaaaggact catatgcttc gttgcgctcc
      121 atcgcctcgc tccaacgtac tggctaagta cgccaccgct cgtcgatttt tcgcgcgcct
      181 tgcctctgag acctttttga gcagccttca acagaggttt tcactactgc tggccgatga
      241 tcaaagtagt tgacctgcac aagaccttcg gtggccaagc ggtcctcaaa ggtatcaatc
      301 tggagctcga aaccggcaag gtcacaacga tcatcggtgg cagcggttcc ggcaagaccg
      361 tgttgttaaa gcatttgaat gcccttcttc tgccgaatca cggcagcgtg ctggtggacg
      421 gaaaagatat caccaagctg ggcgagcgcg aactcaatga agtcagacag aaatttggcg
      481 tcctctttca aggcgccgcc ctgctcgact ccatgaccct ctatgacaat gtcgctttcc
      541 ctttgcgcga gaaaaccaag atgtcggaaa acgagatccg aaagagagtt gaggagcgac
      601 tagcccaggt tggacttgcg ggtatgggct acaagtaccc agccgaggtc agcggcggca
      661 tgaaaaaacg cgccggactg gcccgcgcct tggccatgga acccgagatt gtgttgttcg
      721 acgagcccac gaccggattg gaccccttgc tgggcaagag cattcaccga ctcatcggta
      781 aaatgcatgc cacattcgga ttcacgggag tcattgttag tcatgatatt ccggaagtgt
      841 tcaagatctc ggatcgggtt gctatgttgg cgaatggagt gatcgcggag gcgggttcga
      901 cgcaagaatt cgtggcgtcg aaaaatccgg tggttcggca gttcctgcaa ggagatgccg
      961 aaggtccact cacggttcta taggagattg atcagtgacc cagacacgta tggaaataat
     1021 cgtcggcctt ttcgttttgg tgggaatggc ctgcctcggt tatctagcca tcaagctcgg
     1081 gaagctcgaa gtattgggaa atagcggcta cccggtatat gcggatttct catccgtagc
     1141 agggctcaag atgggtgacc cagtggaaat tgccggcgtg aaaatcgggc gggtggaagc
     1201 aatggggctg gtcgatgatc gcgcccggct ccaactacga cttcaggacg gcgtcaagat
     1261 ccaggaagac gccatcgcgt cggtacgagc ccgaggattg atcggcgata agttcgtcct
     1321 gatcacaccg ggagcatccg ataagatcat cccacctggc ggaaggctcc gggaaaccga
     1381 atcaccgccg gatattgcgg acttgatcgg gaagattgtc gggggagatt taacgtcaaa
     1441 gagtgaaaag aaagaggaaa gccagaaata gtttaaagat cggagcgacc gtagttccat
     1501 tcctagcggc agccttggtc accgtgcctg cagcctctgt tcatcaagtg cgttgaacgg
     1561 gctcacccca tcacaaccgc gcttgactct gcaagccctt ccgcagtatc gaaagcttga
     1621 aagcggatac gaaagggagc ccaccatggc gacttggatt gattggcact ctcatcatac
     1681 ggcccctgaa gcggtagaga catttgccga gttcaccggg aaactgccga agatcgacga
     1741 ttacgactcc atcgactttg cagaccgcgt gcagcagatg gatgaggtcg gcatcgacct
     1801 ccaactgatc tgccagggcg cgggcttgta cgctgaccaa ctgcccgccg accaggcgtt
     1861 ggccatcgtt cgccaatcca acgatctgct cgcccaacgt atcgcgccac atcggcagcg
     1921 gcttatggga gtcacggctc tctcgctaaa aaatatcgag gcgtcggtgg aggagctgga
     1981 gcgcacttcg acccggggct ttcgcgcggc tttgctgtat cctaccgtag acggcgaaat
     2041 ggttgtggac ctgccggtaa tggacccgct ttacgccaag attgccgaac tggaatggcc
     2101 catctttctg cacggcgccg gtcttgccaa agacccaacg ctcaaacggc tcgaagatgg
     2161 aggagcgggg gtcgcgttcg ccgtcctttc cgatgccgag atctcggagt gcgcgttgcg
     2221 tatgatcgcc ggcggcgttt tcgatcgcta tccgggtttg cgaattgtca ttcgcagttc
     2281 cggcggcggc ctccccttgc ttctgcatcg ctttttttgg aaacataagg gacccagagg
     2341 tgagcagcgc tattcagaaa tttttctcga gcacttcttg atcgattgtg cgagctccga
     2401 cgcccgaacc ctgtcgtttt tgatcgacac catgggggaa gagcacgtgg ttttcgggtc
     2461 tgactattgc ggcggcctgg gcccattgaa aaaggctcta gcatctgtag aaaaccaaac
     2521 taatccggaa tatttgaaga gcttcactga aaagaattcc cgccggctgc ttcacattta
     2581 gagagcggct atttatgacc tcg
//
LOCUS       DASSWZ010000239         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1240033, whole genome shotgun sequence.
ACCESSION   DASSWZ010000239 DASSWZ010000000
VERSION     DASSWZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240033"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VFN58_05780"
     CDS             complement(<1..1200)
                     /locus_tag="VFN58_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8917534.1"
                     /translation="MSALREAFKEFTQYPSAIMGLVIIAALIVLSIYTVIVIPYAEGM
                     RLWRGGEDIWIENPRNARPRWVNFFLKEHLPQTIIVNTPPGAAKVGKPLPGGLRALDM
                     TLSFDYPYDAFPSEINLFLKAKFKESRPYVSFLWLTPDGRRIPLGERSVRGSERFSIS
                     LDRNLAQRLGRRPPEIGLFTRPDATIENSVPLNGRYQLQLEGFFFEDGSDLEAKLVVY
                     GQVYGLAGTDHRRRDLMVALLWGTPVALAFGFLAAIGSTLTTLLIAAIGVWFGRWVDA
                     VIQRVTEVNAILPVLPILIMIGIFYSRSLWVMLGAIILLSIFSLGIKTYRSILLQVKE
                     SPYIEAARTYGTGNFRIIFRYMIPRIIPVLIPQFVTLIPSFVFLEAGLAVLGLGDPVL
                     PTWGKVVN"
     gene            complement(1197..2276)
                     /locus_tag="VFN58_05785"
     CDS             complement(1197..2276)
                     /locus_tag="VFN58_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8917535.1"
                     /translation="MTFIRLLRYVVLRAASLFITVVVGVYLAILIANMGGYVDEIRKA
                     QIREQIGATMLADQKLRTMPPSEVRRVIDQQIELEEKRLGLDRPFLVRSFAFLRDALT
                     LSLGRAERLTSDTGSRQVRDIILERLPATLLLFGTADLIVFFTAIFVALFLSRRYGSW
                     LDKIVVALAPTSSAPAWFYGIFLILIFASVFRILPFGGMVDVPPPESALEYGLSVLQH
                     LILPVSAILVGSLFISIYNWRTFFLIYSSEDYVEVAKAKGLPSGAIERRYILRPTLPP
                     IITSFAFALISLWSGAIILETVFSWPGLGRLLFQAVGLFDTPVIIGSVVIYAYLLALT
                     VFLLDILYALVDPRVKVGGEAGRSL"
     gene            complement(2290..>3103)
                     /locus_tag="VFN58_05790"
     CDS             complement(2290..>3103)
                     /locus_tag="VFN58_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917536.1"
                     /translation="GVLAERNRQLAFSASKADRLRVQWLSYISGPSLAVLDRHLAAAL
                     KEGFVPYRNTLGEYISQQEATERYEKLRAWRQTRQHFWVSNGPFYLYSVHPVEKIVVI
                     RRSESFSDPADKWVRFVEPQIADVEVSGPRIVKIGTPAEFQVQISFKGMPYPEKDLEF
                     VRYMVFDSRGQLVELANPESAGNGMWRITLSPKQTARLSSGSNRLELAVTSRLVALPS
                     FRTFRFVTVKEETKLGSHWKTEGKFLTDRPIERDESRRAILNFLPSENVPGS"
BASE COUNT          788 a          867 c          827 g          621 t
ORIGIN      
        1 gttcaccact ttgccccagg tcggcagcac cggatcgcct agacccaaga cggccaaccc
       61 ggcctcgaga aagacaaacg acgggatcag ggtaacgaac tgcgggataa gcaccgggat
      121 gatgcgcggg atcatgtagc ggaaaatgat ccggaaattg cccgtcccgt aggtccttgc
      181 cgcctcgatg tatggggatt ctttaacctg caagaggatt gaacgataag tcttgatacc
      241 aaggctaaaa atgctcagga gtataattgc tccgagcatt acccagagac tccgtgaata
      301 aaagatgccg atcataatga ggatgggcag gaccggcagg atcgcgttca cttcggtgac
      361 ccgttggatg acagcatcga cccagcggcc gaaccatacg ccgatagcgg cgatcaacaa
      421 agtcgttaat gtagatccta tggcggccag gaaaccaaac gccaaggcca ccggagtgcc
      481 ccaaagcagc gccaccatca agtcgcgacg acgatggtcg gtgcccgcca gcccatagac
      541 ctgcccatag actaccagtt tcgcttcgag gtcggagccg tcctcgaaga aaaacccttc
      601 aagctgcaac tgataccgtc cgttgagcgg cacagagttc tcgatggttg cgtctggcct
      661 ggtaaataat ccgatctcag gaggacgacg tccaagtctt tgcgctagat tgcggtcaag
      721 ggaaatactg aaacgctctg acccacgcac gcttcgctcc cctagaggga tacgacgccc
      781 gtcaggtgtt aaccagagaa aggaaacata gggccgactc tccttgaatt ttgcctttag
      841 gaaaaggttg atctcgctgg gaaaggcgtc atagggataa tcgaaactga gcgtcatatc
      901 taacgcccgt agacctccag gcaacggttt tccgaccttc gctgccccgg gcggcgtgtt
      961 cacgattatg gtctgaggga ggtgctcttt taggaaaaaa tttacccagc gtggccgggc
     1021 atttctcggg ttctcgatcc agatgtcttc gccgccgcgc cagagccgca tgccttccgc
     1081 gtaaggaata acgatcaccg tgtaaatcga cagcacaatc agcgccgcga tgatcaccag
     1141 ccccatgatc gcggagggat attgggtaaa ctctttgaat gcttctcgga gcgcgctcac
     1201 agacttcttc ccgcctctcc tcccaccttc acccggggat ccaccagcgc gtagagaatg
     1261 tccagcagga agacagtcaa ggcaagcaag taggcataga tcaccacgga gccgataatc
     1321 accggggtat cgaataggcc cacggcctgg aaaagcagcc tgcccagacc gggccagctg
     1381 aagactgttt ccagaataat cgccccgctc caaagcgaga tcaaagcgaa ggcaaagctc
     1441 gtgatgatgg gcggaagagt cggccgcaga atgtagcgcc gctcaatggc tcccgagggt
     1501 aaccccttgg ctttggccac ctccacgtaa tcttcgctgg agtaaatcag aaaaaaggtt
     1561 cgccagttgt aaatcgagat aaagagcgaa ccgacgagga tcgccgacac cggcagaatc
     1621 aggtgttgca gcacgctcaa gccatattcc agagccgatt cgggtggcgg aacatcgacc
     1681 attccgccaa aaggcaggat gcgaaacacc gaagcaaata tgagaatcaa aaagatccca
     1741 taaaaccagg ccggagcgga agaggtgggc gcgagcgcga ctactatctt atccagccaa
     1801 cttccataac gccgggacaa aaagagcgcc acaaagatgg ccgtgaaaaa aacgatgagg
     1861 tcggctgtgc cgaaaagcag cagagtggcc ggcaagcgct caagaatgat atcgcgcacc
     1921 tgtctcgatc ccgtgtcgct ggtgagccgc tccgctcttc ctaaagagag agtgagcgca
     1981 tccctcagga aggcaaagct gcgtaccaga aagggccgat ctaaccctag acgcttttcc
     2041 tccaactcga tctgctggtc gatgacccgg cggacttcac tgggtggcat agtccgaagc
     2101 ttttggtcgg ccaacatggt cgccccgatc tgttcgcgaa tttgcgcttt gcggatctca
     2161 tctacatagc cgcccatatt ggcgatgagg atggcaaggt acactccgac gaccaccgtg
     2221 atgaataacg atgcggctcg gagtaccacg tacctcagaa gccggatgaa agtcatgact
     2281 attggaccat taactaccgg gaacattctc cgacggtaga aaattgagga tggctctcct
     2341 agattcgtct ctttctatcg gtcggtctgt caaaaacttg ccttcagttt tccagtgcga
     2401 ccctaacttc gtttcttctt tgacggtcac aaatcggaaa gttctaaaag aaggcagagc
     2461 caccagccgg gacgtcacag ccaattcaag ccggttggag cctgatgaga gacgcgccgt
     2521 ttgcttcggg ctgagcgtga ttcgccacat tccgttgcca gcggactcag ggttagccaa
     2581 ctcaaccagc tggccgcgtg aatcgaacac catgtagcgg acgaactcca agtctttttc
     2641 cggatagggc attcccttaa agcttatttg tacttgaaac tccgcagggg ttccgatctt
     2701 aacaattctc ggacctgaaa cctcgacgtc cgcgatttgt ggctcgacaa atcgcaccca
     2761 tttatcggcc ggatcggaaa aagattcgga tctcctgata actacaatct tttccaccgg
     2821 atggactgag tatagataaa atggtccatt gctgacccag aaatgctgtc gagtctggcg
     2881 ccaggctcgg agtttctcat agcgctccgt cgcctcctgc tgactgatat actcacccag
     2941 tgtgttgcgg tagggtacga agccctcctt gagagcagcc gcaaggtggc gatccaggac
     3001 ggccagactc ggcccgctga tatagctcag ccactgaact ctaaggcggt ccgccttgga
     3061 ggcggaaaaa gccaactgtc tatttctttc cgccaatacc ccg
//
LOCUS       DASSWZ010000240         7649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_772849, whole genome shotgun sequence.
ACCESSION   DASSWZ010000240 DASSWZ010000000
VERSION     DASSWZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772849"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..350
                     /gene="rpsS"
                     /locus_tag="VFN58_05795"
                     /pseudo
     CDS             87..350
                     /gene="rpsS"
                     /locus_tag="VFN58_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525977.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            524..868
                     /gene="rplV"
                     /locus_tag="VFN58_05800"
     CDS             524..868
                     /gene="rplV"
                     /locus_tag="VFN58_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET8917537.1"
                     /translation="MEAKAITKYVRLSPRKVRLVVDQIRGKGVEEALNILKFVPKRSA
                     GIVAKTLHTAVANAENTQSVDVDRLYVKQVKVDEAGMWKRFTPRAQGRATRIRKRLSH
                     ITIVLDEVTERS"
     gene            875..1537
                     /gene="rpsC"
                     /locus_tag="VFN58_05805"
     CDS             875..1537
                     /gene="rpsC"
                     /locus_tag="VFN58_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET8917538.1"
                     /translation="MGQKTHPRVFRLGVIETWDSKWFARHDYPKLLHEDFRVKKFLKI
                     RLYHAGISRIEIERAANKAKINIYTARPGIVIGKKGAEIEKLKEEITKLTNRDTYINI
                     HEVRRPDLDAQLVAENVALQLERRVAFRRAMKESVSRAMRMGAQGVRIQCAGRLGGSE
                     IARTEWYREGRVPLHTLRADINYGFAEARTTYGIIGVKAWIFRGEVLTEEEEQQKAML
                     GG"
     gene            1579..2004
                     /gene="rplP"
                     /locus_tag="VFN58_05810"
     CDS             1579..2004
                     /gene="rplP"
                     /locus_tag="VFN58_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET8917539.1"
                     /translation="MLEPKKVKYRKLQKGRRRGTAWRGSSLAFGEYGLKAIERGWMSA
                     REIEAARVALTRYLKRGGKVWIRIFPDKPLTKKPAETRMGKGKGAPEMWVAVIQPGRI
                     LFEMEGVDGPLAREAFRLAAHKLSIPTIFVERRGLAHGG"
     gene            1994..2203
                     /gene="rpmC"
                     /locus_tag="VFN58_05815"
     CDS             1994..2203
                     /gene="rpmC"
                     /locus_tag="VFN58_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET8917540.1"
                     /translation="MEAKELRDMSTDDLTQKRTELREEIGHLKLKRATSRLENPMKLG
                     QTKRDLARVETILREKAFQSGKGAA"
     gene            2200..2493
                     /gene="rpsQ"
                     /locus_tag="VFN58_05820"
     CDS             2200..2493
                     /gene="rpsQ"
                     /locus_tag="VFN58_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET8917541.1"
                     /translation="MNERGLRKERMGLVISDRMHKTVIVSVERTVMHPRYKKYLRRRT
                     KVKAHDERNQSHVGDRVLIVECRPLSRDKRWRVSRILARAKTTEADESKAAAV"
     gene            2514..2882
                     /gene="rplN"
                     /locus_tag="VFN58_05825"
     CDS             2514..2882
                     /gene="rplN"
                     /locus_tag="VFN58_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET8917542.1"
                     /translation="MIQMRTVLDVADNSGARKVQCIKVLGGSKRKYASIGDIIVVSVK
                     EAIPNAKVKKGEVMKAVVVRTAKEIGRPDGTYIRFDNNSAVLIDNQKEPIGTRIFGPV
                     ARELRAKKFMKIISLAPEVL"
     gene            2907..3218
                     /gene="rplX"
                     /locus_tag="VFN58_05830"
     CDS             2907..3218
                     /gene="rplX"
                     /locus_tag="VFN58_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET8917543.1"
                     /translation="MQIRKNDSVMVISGKERGKTGKVLRVLSNKDAVVIERVNMVKRH
                     SRPRGPQQPGGIVEKEAAIRVSNIMMMCDKCNAPVRVGRKILADGKKIRICRRCGEAL
                     D"
     gene            3230..3769
                     /gene="rplE"
                     /locus_tag="VFN58_05835"
     CDS             3230..3769
                     /gene="rplE"
                     /locus_tag="VFN58_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET8917544.1"
                     /translation="MARLKQKYRDEVVAAMMKEFGYKNSMQVPRLEKITVNVGLGEAT
                     QNIKVLDTAVAEVTAITGQKPVVTRAKKSIANFKLREGVPIGCMVTLRQERMYEFLDR
                     LIHVALPRVRDFKGVSDRSFDGRGNYSLGLREQIIFPEIQADKVEKTRGMTVSFITNA
                     KTDQEGRTLLKLLGMPFVS"
     gene            3789..3974
                     /locus_tag="VFN58_05840"
     CDS             3789..3974
                     /locus_tag="VFN58_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET8917545.1"
                     /translation="MAKTCLRIKAERPNKFKVREYNRCPLCGRARAFYRRFRMCRLCL
                     RDHARKGHIPGLIKASW"
     gene            3990..4394
                     /gene="rpsH"
                     /locus_tag="VFN58_05845"
     CDS             3990..4394
                     /gene="rpsH"
                     /locus_tag="VFN58_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET8917546.1"
                     /translation="MGMTDPIADMLTRIRNASRARHPKVAIPWSRVKENIIKVLIEEG
                     YLRESKKVKASEGAGEELVIQLKFDRENRPVIVGIKRVSRPSRRVYVGAQAMTPIRKG
                     LGINVLSTPRGILVDREAQRANVGGEVICSVW"
     gene            4412..4963
                     /gene="rplF"
                     /locus_tag="VFN58_05850"
     CDS             4412..4963
                     /gene="rplF"
                     /locus_tag="VFN58_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET8917547.1"
                     /translation="MSRIGKLPITIPSGVKVVVEPKEVRLEGPKGKLKTTIPPGVNVK
                     VEGPIVRVEREGEQRQLRALHGLTRRLIANMAQGVSEGFSRVLEINGVGYRAEVKGQE
                     IHITLGFSHPVVFPLPQGVAASVERQVIVTLSSADRQLLGETAAKLRGLRPPEPYKGK
                     GVKYREEVIRRKAGKAVGSAGSA"
     gene            4983..5348
                     /gene="rplR"
                     /locus_tag="VFN58_05855"
     CDS             4983..5348
                     /gene="rplR"
                     /locus_tag="VFN58_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET8917548.1"
                     /translation="MANKQKDIARTRRKQRVRKKVLGTDQRPRISVFRSNKHIYAQVI
                     SDDRGMTLASISTLSKDLAAKVENIKGVEAAKQVGLALAKICKEKSITQVIFDRNGFL
                     FHGRVKAIADGAREGGLKF"
     gene            5373..5870
                     /gene="rpsE"
                     /locus_tag="VFN58_05860"
     CDS             5373..5870
                     /gene="rpsE"
                     /locus_tag="VFN58_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET8917549.1"
                     /translation="MERIDPQGLELKEKVVHISRVAKVVKGGRRFSFSALVVVGDNHA
                     VVGYGMGKANEVPEAIRKGLEQAKKNLIRVPVMEGTIPFEVSGRFGAGYVILKPASDG
                     TGVIAGGGVRAVVEAAGIQNVLTKCLGSNNPHNMVKATVEALKDLRPPEEIADRRGKV
                     LAEIR"
     gene            5873..6325
                     /gene="rplO"
                     /locus_tag="VFN58_05865"
     CDS             5873..6325
                     /gene="rplO"
                     /locus_tag="VFN58_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET8917550.1"
                     /translation="MKLSDLGPAQGSNNKRKRVGRGDGSGHGKTSCRGHKGQGARSGG
                     GTRPGFEGGQMPLQRRLPKRGFHNPFRVETAIVNLEQLEAFPSGSEVTPALLVEKGLL
                     RSKNQRVKILSDGALTKPLTVKAHGFSAKAKEKIEACGGKLELLGAHA"
     gene            6318..7628
                     /gene="secY"
                     /locus_tag="VFN58_05870"
     CDS             6318..7628
                     /gene="secY"
                     /locus_tag="VFN58_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET8917551.1"
                     /translation="MLNGFQNASKIPELRRRVLFTLAMLAVYRLGVHVPTPGIDRQAL
                     AAYFEQVKGTIFGLFNLFSGGALEQFSVFSLGIMPYISASIILQLLTVVFPYIERLSK
                     EGEAGRRKITQYTRYGTVVLSIIQGLMISFGLEGTRGPGGAAIVLDPGWSFRLMTVIT
                     LTSGTAFIMWLGEQITERGIGNGISLIIFAGIVARLPSALTTTFQFIREGELGVLIAL
                     FLLVFTVVIVGVIIFMERGQRRIPVQYAKRVVGRKMYGGQSSHLPLKINTAGVIPPIF
                     ASSILIFPATVAQFAPYPWVQSISGMLSPGQWLHDLVYVVLIIFFAYFYTAVVFNPVD
                     VAENMKKFGGYIPGIRPGQKTAEFIDRVLSRITLGGALYISVVALLPTILIQQFNVPF
                     YFGGTALLIVVGVALDTVAQMETHLITRNYEGFMKRGRLRGRRG"
BASE COUNT         1884 a         1768 c         2375 g         1622 t
ORIGIN      
        1 tggggcaagc ctaccaaggg atacaaaacg agaaagaatc atgcgtccga caaatatatc
       61 gtgaagggca gaaaggctaa gtaacgatgg cacgttcggt caagaaagga ccgttcgtcg
      121 acgcgcattt ggcgaagaag attgcggcga tgaacgaatc tcgccagaaa cgggtaattc
      181 agacttggtc tcgccgttcg acagttacgc cggaaatggt cggttatacg attggcgtcc
      241 acaacgggcg aaaatttatt cctgtatttg ttacagaaaa catggtgggc cataagctcg
      301 gggaattttc tccgacccgg accttctttg tacactccgg cgatcgcaaa ggggaagcca
      361 aggagacggt gggggcggtt gcgcccgcgc ccggggctcc agcggccgga actgcgaccg
      421 cggctccgag accggctgcg gcgcctacgg ctcccaagag ttgaatggat tcaccgcgag
      481 cggagaacct gacgaaattc aagtaaccaa ggccgtcgaa accatggaag ctaaagcgat
      541 cacaaaatat gtccggctct ctccgcgcaa agtgcggctg gtggtggatc agatacgcgg
      601 taagggagtc gaggaggcct tgaatattct caagtttgtt cccaagcgct ccgcgggaat
      661 tgtcgcgaag accttgcata cggcagtagc caatgcggag aatacccaaa gcgtcgatgt
      721 ggatcgtctt tatgtaaagc aagtaaaggt ggatgaagcg ggtatgtgga aacgattcac
      781 gcctcgagcg caaggcagag ccacacgcat ccgcaaacgc ctgagtcata tcactatcgt
      841 gttggacgag gtcactgaga ggagttgaag gctgatgggt caaaaaacac acccgagagt
      901 ttttcgtctt ggcgtaatcg agacttggga ctcgaagtgg tttgctcgtc atgactatcc
      961 taagctgctc catgaagact ttagggtcaa aaaattctta aaaattcgtc tttaccatgc
     1021 gggcatctcg aggattgaaa tcgaaagagc agctaacaaa gcgaaaatta acatttacac
     1081 agctcgcccg ggaattgtca ttggcaaaaa gggtgcggaa atcgaaaagc tgaaggaaga
     1141 gatcaccaaa ttgaccaacc gcgacacgta cattaacatc catgaggtaa ggcgaccgga
     1201 cctcgatgct cagcttgtcg ctgaaaacgt tgcgctacag ttggaacggc gggttgcctt
     1261 ccgacgcgcc atgaaagaaa gtgtctctcg ggcgatgcga atgggcgccc agggcgtgag
     1321 aattcagtgt gccggccgat tgggaggatc ggaaatcgct cgcacggagt ggtaccgcga
     1381 agggcgcgta ccgctgcaca cattgcgtgc cgatatcaat tacgggtttg cggaggcacg
     1441 cacgacctac ggtattattg gcgtaaaggc ctggatcttc agaggcgagg tcttgaccga
     1501 agaggaagag cagcagaagg cgatgttagg gggttgagag acggtcgcta ggcatcgtaa
     1561 cacttcaaaa ctcataatat gctagaaccg aaaaaagtaa aataccggaa attgcaaaag
     1621 gggcgtcgac gtggcactgc gtggcgcggc tcttctctgg ctttcggtga gtatgggctc
     1681 aaggccattg agcggggttg gatgagcgca cgcgaaatcg aagcggctcg agtggctctc
     1741 acccgttatc tcaagcgcgg tggtaaggtc tggattcgaa tttttccgga caagccgtta
     1801 accaaaaagc cggcggaaac tcgtatggga aagggaaagg gtgctcccga gatgtgggtt
     1861 gcagtgattc agccggggcg catattgttc gagatggagg gtgtcgacgg gccgcttgcc
     1921 cgagaggctt tccgtctcgc tgcgcacaag ctgtcgattc cgacgatctt tgtggagcgg
     1981 agagggctcg cacatggagg ctaaagaact gcgcgatatg agtacggacg atttaactca
     2041 gaagcgtaca gaacttcggg aggaaatcgg acacctgaaa ctgaagcggg ccaccagcag
     2101 gctggaaaac ccaatgaagt tagggcaaac gaaacgggac ttagccaggg tggagaccat
     2161 ccttcgagag aaggcgtttc agagcgggaa aggcgcggca tgaatgagag aggactcaga
     2221 aaagagcgta tggggttagt gattagcgac cggatgcata aaaccgtcat tgtttctgtg
     2281 gagcggacgg tgatgcaccc aaggtataag aagtacctga ggcgcagaac taaggtgaag
     2341 gctcatgatg aacgcaacca gagccatgtc ggagaccggg tgttgattgt cgaatgccgg
     2401 ccgctgagtc gggacaagcg gtggcgcgtc agtcggatcc tggcacgagc caaaacgacg
     2461 gaggccgacg agtcgaaggc agccgctgtc tgagccgtcg ttgaggttaa ttcatgattc
     2521 agatgagaac ggtgttggat gtggcggata actcgggggc gcgcaaggtg cagtgcatca
     2581 aagtcctggg gggcagcaag cggaaatatg cgtccatcgg agatattatt gtggtttccg
     2641 tcaaggaagc gattcccaat gccaaggtaa aaaagggcga ggtgatgaag gccgtggtgg
     2701 ttcggacggc aaaggaaatc ggaaggccgg atggaaccta tatccgattc gacaacaatt
     2761 cggctgtgct gatcgacaat caaaaggagc cgatcggcac ccgcatattt ggaccggtag
     2821 cgcgcgaact acgggccaag aaattcatga agattatttc gctcgcaccc gaagtccttt
     2881 gatcagttat cgaggagaaa tcgctcgtgc agattcgcaa gaatgacagc gtaatggtga
     2941 tctcgggaaa ggagagagga aaaaccggca aagtccttcg cgttctctcc aataaggacg
     3001 ctgttgttat cgaaagagtc aatatggtta agcggcacag tcggccgcgg ggtccacagc
     3061 aacccggggg aattgtggaa aaggaagctg ccattcgcgt ttccaacatc atgatgatgt
     3121 gtgacaagtg caacgcgccg gtgcgcgtcg ggcgaaagat tctagcggac gggaaaaaaa
     3181 tccgtatctg ccggcggtgt ggcgaagcct tagattaggt agggatagaa tggcgcgact
     3241 gaagcagaag tatcgagacg aagtggtggc ggcgatgatg aaggagttcg gctacaagaa
     3301 ctccatgcag gtgcctcggc tggaaaaaat taccgtgaat gtcggactcg gagaggccac
     3361 gcaaaatatc aaggttctcg ataccgccgt agccgaggtc accgccatca ccggacaaaa
     3421 gccggtggtg acgcgcgcta aaaagtccat cgctaacttc aagctgcgcg aaggggttcc
     3481 gattggttgc atggtcactt tgcggcagga gcgcatgtac gagtttctcg accgactgat
     3541 tcatgtcgca ctgcctagag ttcgcgattt caagggggtc tcggatcggt ccttcgatgg
     3601 ccgcggcaat tattctctcg gcttgcgcga gcagattatt tttcctgaga tccaggcgga
     3661 caaggtcgag aaaacacgag ggatgaccgt ctcgttcatc accaatgcga agacggacca
     3721 agaaggtagg actctgctca agttgctggg tatgcctttt gtcagttgat ccatgagggg
     3781 gattgccatt ggccaagaca tgtcttcgaa ttaaagcaga gcgaccgaat aaattcaaag
     3841 ttcgggaata taatcggtgt cccctttgtg gtcgggcgcg ggcgttttat cggcgcttcc
     3901 gtatgtgccg gctctgtctg cgtgatcatg cgcgcaaggg gcatattccg gggctgatca
     3961 aggccagctg gtaaagggcg gagagtacga tgggaatgac cgatccgatt gccgatatgc
     4021 tgactcgtat acgcaacgct tcccgggcgc ggcatccaaa ggtagccatc ccgtggtcgc
     4081 gtgtgaaaga aaacatcatc aaggttttga ttgaggaagg ctacttaaga gaatcgaaaa
     4141 aggtaaaggc ttccgagggc gcgggggagg agcttgtgat ccagctcaag ttcgacaggg
     4201 aaaatcgtcc cgtgatcgta ggcatcaagc gcgtcagccg gccgagccgc agggtctacg
     4261 ttggcgcgca ggccatgacg ccgatacgaa aagggttggg aatcaacgtg ctctcgacgc
     4321 ccagagggat attggtggat cgggaagctc agagggcaaa cgtcggagga gaagtcatct
     4381 gttcggtatg gtagtttagg cgaggtgtag agtgtcgcgt attggaaaac tgcccataac
     4441 gatcccaagt ggagtcaagg tggtcgtcga acccaaggaa gttcgtttgg aaggaccgaa
     4501 aggaaagctc aaaacaacta ttccgccggg ggtcaatgta aaagttgaag gtcctatcgt
     4561 tcgagtcgag cgggagggcg agcagcgtca gcttcgggct ttacatgggc tgacgcgaag
     4621 gctgatcgcc aatatggccc agggtgtcag cgaggggttt agtcgcgtgc ttgaaatcaa
     4681 cggcgtcggc tatcgcgcgg aggtcaaggg ccaagagatc cacatcaccc tgggcttttc
     4741 tcatccggtg gtgttccctt tgccccaagg ggtggccgct tccgtggaga gacaggtgat
     4801 cgtaacactc agcagtgcgg atcgtcagtt actcggagag accgccgcga aactacgtgg
     4861 cctcagacca cccgagccat acaaaggcaa gggtgtaaaa tatcgtgaag aagtgattcg
     4921 gcgtaaggcg gggaaagcgg ttggttccgc gggcagcgcg tagtatttga gggcaagaac
     4981 agatggcaaa caagcagaaa gacattgccc gtacgcggcg aaagcaacgg gttcgtaaga
     5041 aggtcttggg gacagatcaa cggccgcgca tttctgtctt tagaagtaat aagcatatct
     5101 atgcgcaagt catttccgac gatcgcggga tgacgttggc atcgatctcg actctctcca
     5161 aagatttggc ggccaaggtc gagaatatca aaggcgtaga ggcggctaag caggttggac
     5221 tggcgctggc caagatctgt aaagaaaaga gtattacgca ggttatcttc gatcgcaacg
     5281 ggtttctctt tcacgggcgg gtcaaggcca ttgccgacgg cgctcgcgaa ggcggattaa
     5341 aattttaaca ggatttattg agcgaggttt ctttggaacg gattgatcca cagggtttgg
     5401 agctgaaaga gaaagtggtg cacattagcc gggtagccaa agtcgttaag ggcggccggc
     5461 gattcagctt cagcgcgctg gtggtggtag gtgataacca cgccgtagtc ggttacggaa
     5521 tgggcaaggc gaatgaagta ccggaagcga tacgaaaggg actagagcag gccaagaaaa
     5581 atctgattcg ggttcccgtt atggaaggaa cgatcccttt cgaggtcagt ggaaggttcg
     5641 gggccgggta cgtcattctc aaacccgcgt ccgatggcac tggagttatc gccggtgggg
     5701 gcgtgcgagc ggtggtggaa gcggcgggaa tacagaatgt tttgaccaag tgtctcggct
     5761 ccaacaatcc acacaacatg gttaaggcga cggttgaggc gctcaaggat ttgcgccctc
     5821 ccgaagaaat cgcagaccgg cgcggaaagg tactagcgga gattcgctga gtatgaaact
     5881 aagtgatcta ggaccggcgc agggttccaa caataaacgg aaacgcgtcg gccgaggaga
     5941 cggctccggc catgggaaga cctcgtgccg aggccacaaa gggcagggcg cccggtctgg
     6001 agggggaacg cgccccgggt tcgaaggggg ccagatgccg ctccagcggc gtcttcccaa
     6061 gcgcggtttc cataatccct ttcgagttga gacggcgatc gttaatcttg aacaactgga
     6121 agctttcccg tcggggagcg aagtaacgcc cgcgctgctc gtcgaaaaag ggcttttgcg
     6181 gagtaagaac caaagggtca agatcctcag cgacggggcc ctgacgaagc cactgacggt
     6241 taaggcccat gggttctctg ccaaggctaa agagaaaatc gaagcctgcg gcggcaaatt
     6301 ggagttgttg ggagctcatg cttaacggtt tccagaacgc atcgaaaatt cccgagctca
     6361 ggcgtcgggt cctctttacc ctggcgatgt tggcggtgta tcggctcggt gttcatgtac
     6421 caactccggg aatcgatcgt caggcactgg ccgcctattt tgagcaagtc aaaggaacga
     6481 ttttcggact tttcaatctc ttttccggcg gtgcccttga gcaattttcg gttttttcct
     6541 tgggcatcat gccctacatc agcgcgtcga tcattctcca gttgttgacg gtggtttttc
     6601 cttacattga aagactttcg aaggaaggcg aagccgggcg ccgcaaaatc acacagtata
     6661 cacgctatgg aactgtggtc ttatcgatca ttcagggact catgattagc tttggactgg
     6721 agggaacgcg gggtcctggg ggtgccgcca ttgtgttaga tccgggttgg agctttcggt
     6781 tgatgacagt catcacactg acatccggca cggctttcat tatgtggctg ggtgagcaaa
     6841 ttaccgaacg tggaatcggc aacggcatct cgctgatcat ttttgccggc atcgtggccc
     6901 ggctcccgtc ggcgcttacg acaacctttc agttcattcg cgaaggtgaa ttgggagtct
     6961 tgatcgcgtt gtttctgctc gttttcaccg tcgtcattgt gggagtcatt attttcatgg
     7021 aacgaggaca gagacgcatt cctgtccagt acgccaaacg ggtggttgga cgaaagatgt
     7081 acggtgggca aagctcccac ttgccgttaa agatcaatac ggcgggagtg attccgccga
     7141 tttttgcctc gtccattttg atctttcccg ccacggttgc gcagtttgcg ccctacccct
     7201 gggtgcaatc catctcgggc atgttgagcc caggacaatg gcttcacgat ttggtgtatg
     7261 tggtattgat tattttcttt gcctattttt acactgcggt tgtcttcaat cctgtggatg
     7321 tagcggaaaa catgaaaaaa tttggcggct atattcccgg aatccgaccc ggccagaaga
     7381 cagcggaatt catcgaccga gtgctctccc gcattacctt ggggggggca ctttacattt
     7441 ccgttgttgc gcttttgcca accattttga tccagcagtt taacgtgccg ttctacttcg
     7501 gcgggacagc gctgttgatc gtggtcgggg ttgcactgga cacggtggcg cagatggaga
     7561 cgcatttgat tacccgcaat tatgagggct tcatgaaacg aggtcgcttg cggggaagaa
     7621 ggggctaaag gttggcgagt ttacgcgtc
//
LOCUS       DASSWZ010000241         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1197672, whole genome shotgun sequence.
ACCESSION   DASSWZ010000241 DASSWZ010000000
VERSION     DASSWZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197672"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFN58_05875"
     CDS             <1..322
                     /locus_tag="VFN58_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8917552.1"
                     /translation="VAYYPSVADEPTTRIRPRHPCDAAREIKCPSMILYGGHDNHSTF
                     PVQQRVMESFYANGQPLEWHYFHFGNHGFASTESDGYQPYLADLVWPLVTEFLGRELV
                     GRAL"
     gene            complement(763..1920)
                     /locus_tag="VFN58_05880"
     CDS             complement(763..1920)
                     /locus_tag="VFN58_05880"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET8917553.1"
                     /translation="MGTQTFIPRDYDVFAGLDVDKKSISVTFTDHQGFLKSLNMPYKV
                     EHLVNYVRKHYGDKKVAFAYEAGPTGYGLYDGLEAQAYPCLIASPSMIPKAPGQRVKT
                     NRLDSRGISENLRGGQLKGIHVPTPLYRELRHLTQLRDTLVSEMVRMKQRIKSLLLME
                     GLEFPPAPAGSQWSFLVKNKLRDLPCSTTVRFKLDRLLDSLEFDEKQVLKATREIRRF
                     CQTDPELSQCIEYLKTLPGIGWIVASKLLARVGDWRQIKNIRQLPAFLGLVPTENSTG
                     VRTERGSITHTGDGRLRSKLIQASWSAIRQDGELREFFRTVAKKHPRHLGSRVAIVAV
                     AHKLSVRAAVVLMKQRPYEVRQKVQSAPLTQEETVPQGTTRRLAVPGETNS"
BASE COUNT          435 a          572 c          531 g          483 t
ORIGIN      
        1 cgtcgcctat tacccctcgg ttgccgacga accgacaacc cgcatccggc cgcgtcatcc
       61 atgtgacgcc gcgcgggaga ttaaatgccc gtcgatgatc ctttacggcg gccatgataa
      121 tcacagcacg tttccggtgc agcagcgggt gatggaaagt ttttacgcca atgggcaacc
      181 cttggagtgg cattatttcc actttggcaa ccatggtttt gcatcgactg agtcggacgg
      241 ctatcaaccc tatctggccg atctcgtctg gccgttagtg acggaatttt tgggacgcga
      301 gctggttggg cgggcactgt agctgttgca aaataaaatc acaccttctt cgttatctag
      361 agccgcggca aagtcatttc tatcccacgg gttgaggaag gaagcgaaag aatacgatga
      421 caagaaaaat tctctccgcc gtcctgacaa ttttattttt tgctgctgtt gatcccgccg
      481 aggcgcagca gccgaagaag gtcgcacgga tcgggtatct atcgtcgacc gatccagcta
      541 gtgactccac ccgtgtcgag gcaattcggc tggctgctat gtggatgtca agacactaac
      601 ccacgaatgc tctcttttga ttctcctatg acttttcttc cttacccacc caagagagac
      661 tcgctgctat gcgtggcacg gcacaaagcc gtcacacgga cttgggatgc gcccctcttt
      721 aaggcactac gccccgggct ctctgtctct ggggtcgaac cttcaggagt ttgtttctcc
      781 tggtactgcc agccgtcgag tcgttccctg gggcacagtc tcctcttggg tcaaaggagc
      841 cgattgaact ttttgtctga cctcgtaagg tctctgtttc atcaacacca ccgccgcccg
      901 cacactgagt ttgtgagcca ccgctacaat cgcgacccga gaaccgagat gacggggatg
      961 ttttttggcc acggtgcgaa agaattctct cagttcgccg tcttggcgga tcgccgacca
     1021 agaagcttgg ataagcttac tgcgcaatcg tccgtcaccg gtgtgagtaa tcgagccgcg
     1081 ctcggttctc accccggtgg agttctcggt gggaaccaaa cccagaaaag ccggcagctg
     1141 gcggatgttt ttgatctggc gccagtcccc aacgcgggcc agaagcttgc tggccacgat
     1201 ccatccgatc ccgggaagtg ttttcagata ctcgatacac tgagacagtt ccgggtcggt
     1261 ctggcaaaag cggcggatct ctcgggtggc cttaaggact tgcttctcgt caaactccag
     1321 actgtcgagc aaccgatcga gcttaaagcg cacggtggtc gaacagggaa ggtcccgcag
     1381 tttgtttttg accaagaacg accactggct accggccgga gccgggggaa actccagtcc
     1441 ttccatgagc aacagcgatt taatgcgttg tttcattcgc accatctcgc tgaccaaggt
     1501 gtcacgcagc tgggtcagat ggcgcagctc ccgatacaag ggagtgggaa catggatacc
     1561 tttgagttgt ccgccccgaa gattctccga gatccctcgg ctatcgagcc gattggtctt
     1621 cactctttgt ccgggcgctt tgggaatcat cgacggcgaa gcgatcaagc acggataagc
     1681 ttgggcctcc agcccatcat acaatccgta gcccgtaggt ccagcctcat aggcaaacgc
     1741 caccttctta tcgccatagt gcttacgaac gtagttcaca agatgttcga ccttgtacgg
     1801 catgttcaac gacttaagaa atccctggtg atcggtaaac gttaccgaga tacttttctt
     1861 gtccacatcg agtccggcaa acacatcgta gtcacgcggt atgaacgttt gggttcccat
     1921 cgaagtgcct ccttggttca atggctaaac tttcgaccat tgtatcttgt atggagcgct
     1981 cgcggtgaac cacgacatcc atcatacagg tctctgcgcg a
//
LOCUS       DASSWZ010000242         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_291999, whole genome shotgun sequence.
ACCESSION   DASSWZ010000242 DASSWZ010000000
VERSION     DASSWZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291999"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFN58_05885"
     CDS             <1..733
                     /locus_tag="VFN58_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HET8917554.1"
                     /translation="LMVLEEGVIEGRKVFANILKYIRMGASSNFGNMFSVLGASLWLP
                     FLPMSPIKILTNNLLYDFSQVPIPTDEVDPEQVAKPRPWSMGEIARFILFIGPCSSVF
                     DYTTYAMMWFVFKCNNLGLMPPPELAEHFARASLSNPDSTYAAALFQTGWFVESLLTQ
                     TLIIHVIRTNKVPFIQSRASWPLIVTSGAIMLVGMWLPFSPIGVWLGFVPLPILYWPL
                     LFITLLLYVVLTQVAKMWLVRKLWI"
     gene            complement(880..1365)
                     /locus_tag="VFN58_05890"
     CDS             complement(880..1365)
                     /locus_tag="VFN58_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HET8917555.1"
                     /translation="MRPKAILISVLVLLTGCAEVPLGPSVAVMPAPGKPFDLFVSEES
                     LCRHYAEQQVGISPQQATQNSTATGAVAGTALGAAAGGAIGAAFGAPGPGAAVGAGTG
                     LLTGSAVGANNGYGSTWTIQRRYDIAYQQCMYAKGNQIPGFPAAYTLAPPLPSPPSPP
                     P"
     gene            complement(1389..1838)
                     /locus_tag="VFN58_05895"
     CDS             complement(1389..1838)
                     /locus_tag="VFN58_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917556.1"
                     /translation="MKKVTLALLSVLLMAIALPALGQARPGPFWHGGIEHFHEHDIVV
                     WRGGSWFHGWHGSRIGWWWIVGPAWYWYPAPVYPYPNPYTPPVVVQVPSSPPAQPQAL
                     PPTWYYCDRPPGYYPYVPECGSGWKAVPGTPPPGPASSAPTPPPPPR"
     gene            2320..2640
                     /locus_tag="VFN58_05900"
     CDS             2320..2640
                     /locus_tag="VFN58_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917557.1"
                     /translation="METARNIAGASLAALLFIGAWGANSNAIAAESNAAGAKGVLLKE
                     EYVPDSYCHQKLSAIRESSLAGDHPDLSAEEKIDFYGPCNQDPVGKDQLTKQRLENSE
                     RKSR"
     gene            2833..>3531
                     /locus_tag="VFN58_05905"
     CDS             2833..>3531
                     /locus_tag="VFN58_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET8917558.1"
                     /translation="MLERWLAKYKYYLRFPGLIIAETFYEVFFKTSPETDASTRCGII
                     LAAGEGNRLRSFVENLRGYALPKQYVNFIGKRSMLEHTFYRAQKMIPAERLFTVVSQS
                     HLRHPEVARQLSTRPMACIVEQPDNKDTGPGLLLPLAHLYKRYPDSTVVVFPSDHFIL
                     EEDLFMVYVDQAFRVVEQDPAKVVLLGINPNEAEPEYGYIVPGKKLYGQSAPGTHEVS
                     LFIEKPGQDVARDLV"
BASE COUNT          847 a          923 c          960 g          801 t
ORIGIN      
        1 cctcatggtg ttggaggaag gggtgatcga aggccgcaag gtcttcgcca acatccttaa
       61 atacatccgc atgggcgcca gctcgaattt tgggaacatg ttcagcgtgc taggggcaag
      121 cctttggttg cccttcttgc caatgtcacc gatcaagatc ctgaccaaca acttgcttta
      181 cgatttttcg caggtgccca ttccaacgga tgaggtggat ccagagcaag tggccaagcc
      241 acgaccgtgg tccatggggg agatcgcgcg gttcattctc tttattgggc cctgtagttc
      301 ggtctttgat tacacgacgt acgcaatgat gtggttcgtc ttcaagtgca acaacctagg
      361 gctcatgccg ccgccggaac ttgccgaaca ttttgcccgc gccagtttaa gcaacccaga
      421 ttcgacatac gctgccgcgt tgtttcagac cggctggttc gtcgaatcat tgctcaccca
      481 gactctgatt atccacgtga tccggacaaa caaagtgcct ttcatccaaa gccgcgccag
      541 ctggccgctc atcgtgacct caggggcgat catgctagtc gggatgtggt tgccgttttc
      601 accgatagga gtgtggctgg gctttgttcc tctcccaatc ctctactggc ctctgttgtt
      661 cataacactc ctgctctatg tcgtgctcac gcaggtggca aagatgtggc tggttcgtaa
      721 gctatggatt tgaggatgac cgccacgacg cgtatcagga cctactttcc atcgtcttgt
      781 gctaaacgag ccgatcaagg cattgattgc gtcggagcaa tcaacacaat ctgcgactgc
      841 tttctactaa cggtgtgctc cttttcacga gaaacacgat caaggcggtg gcgagggagg
      901 agacgggaga ggcggtgcta aagtgtaggc cgcgggaaag ccgggaattt gattgccctt
      961 agcgtacata cactgctggt aggcgatatc gtagcgtcgc tggatcgtcc acgtagatcc
     1021 ataaccattg ttcgcaccaa ctgcactgcc tgtgagcaag ccggtcccag cgcccacggc
     1081 cgcacccggt cccggcgctc caaaggctgc gccgattgcc ccacctgctg cagccccaag
     1141 ggcggtgccg gcaacagccc cggttgccgt gctattctgc gtcgcctgct gaggggaaat
     1201 cccaacctgt tgctcggcgt aatgacggca gagggattcc tcgctcacaa aaagatcgaa
     1261 cggtttgccg ggcgcgggca tcacggcgac actcgggcct aagggcacct cggcacaacc
     1321 tgtgagaaga accaggaccg agatcaaaat ggctttcggg cgcatggtga tgcctacgct
     1381 attttaattc accggggtgg aggtggtggc gtcggagcgg acgatgcggg gccgggaggg
     1441 ggagttcccg gcacagcctt ccaaccgcta ccgcattcag gaacataagg ataatagccc
     1501 ggagggcgat cgcaataata ccaagttgga ggcaaggcct ggggttgagc aggaggcgaa
     1561 gagggaacct ggacaactac cggaggagta taagggttcg gatagggata gacaggagcc
     1621 ggataccaat accatgcggg acccacaatc caccaccaac cgatccgact cccatgccat
     1681 ccgtgaaacc agctcccacc gcgccaaacg acgatatcgt gctcatgaaa atgttcgata
     1741 ccaccgtgcc aaaaaggtcc tggtctcgcc tgaccaaggg cgggcaatgc gatagccatc
     1801 agtaaaacag acaagagagc caaagtgact tttttcattg ttgcatctcc ttagaggatt
     1861 tttgcaacgg gcccttgatc gtctctgggc ccgtccaaga aaggtatcta cactatttgg
     1921 acggctaagc attgaaaaag gtttaccgag catcgactca ttttgatttc ctccgtttct
     1981 tgtaaattca aaaacagcaa tgatgatacc aacgagctta aggcttggat gtgttgtgtt
     2041 gtattagtga gagagacgaa agaagtttta gttcgcttcg aataatccgt gaaggaaatg
     2101 aaaagaagac cgtggagatc gactagcaat aaaaccacac tcgctacaca ttgtcgcctc
     2161 actgtgtcga aaaagacggt tgacgctgct gccgagtgac tgcgtcaaaa gaatttttca
     2221 gcaccgaccg ttggccttaa cattgcttaa tatctttgtt ggatggtttg cataaagtca
     2281 catggattga tcgttggctt cttaacactg gaggtagtca tggagaccgc taggaatatt
     2341 gcaggagcgt cactggcagc gctgctcttc atcggcgcgt ggggagcgaa tagcaatgcg
     2401 atcgcggcgg aaagcaatgc tgcgggcgcg aaaggtgtcc ttctgaagga agagtacgtg
     2461 ccagacagtt attgccatca gaagctttct gcgatacggg agagcagctt ggctggggat
     2521 catccggacc tgagcgcgga agaaaaaatc gatttctatg ggccatgtaa tcaggatccg
     2581 gtcggaaaag atcaattgac taagcaaaga cttgaaaact ctgaacggaa gtctaggtga
     2641 ccagagtgta gttctggccc aggccccaca taaccatcac ctaaagatct cttctatccc
     2701 agggtggagg cttgcgtaac ggcgcggtgg cgtttcgtca caaccaagcc tcccccctat
     2761 catacattgt cgcccgcagc cggcgcccaa aaggcgcttc gtcagattgc gcaccgtcca
     2821 agtaaaagag aaatgcttga acgttggctc gcgaaataca aatactacct tcgctttcct
     2881 ggcttaataa ttgctgaaac tttttatgaa gtgtttttca aaacctctcc tgaaaccgac
     2941 gcctcaactc gttgcggcat catattagcc gccggggaag ggaaccggct gcgctcgttc
     3001 gttgaaaatc tacgtggcta cgcgcttcct aagcagtatg tgaacttcat cggcaagcgc
     3061 tccatgctcg aacatacttt ctacagagca caaaaaatga ttccggcaga acgattgttc
     3121 acggtcgtga gccagagcca cctgcggcac ccggaggtag cccggcagct ttcaactcgg
     3181 ccgatggcct gcatcgtcga acaacccgac aacaaagaca cgggtccagg cctgttgctg
     3241 ccgcttgcgc atctgtacaa acgttatccc gattccaccg tggtggtttt tccttcggat
     3301 catttcattt tggaggagga tctctttatg gtttatgtgg atcaagcctt tcgcgtcgtc
     3361 gagcaggatc ccgccaaggt tgtattgcta ggaatcaatc caaacgaagc ggagccggaa
     3421 tacggttata tcgtgccggg aaaaaagctc tacggtcaat cggcgcctgg cacccatgag
     3481 gtttcgctgt ttattgagaa gccggggcaa gatgtcgctc gagatctcgt t
//
LOCUS       DASSWZ010000243         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_942909, whole genome shotgun sequence.
ACCESSION   DASSWZ010000243 DASSWZ010000000
VERSION     DASSWZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942909"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1268)
                     /locus_tag="VFN58_05910"
     CDS             complement(249..1268)
                     /locus_tag="VFN58_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET8917559.1"
                     /translation="MTSNVSSGWGDLSLFSLFRSPFLFPSLLLHAMLFLLVWRAATFS
                     IVRQEPPISVQLLDVSGTGSDTKSIGPGKGPGGPRTPPKLGTPTPPAQRTGKVDTGSL
                     ESSVPSKSVEPEPAPNPVVLPGPKALATDTRHETVNAKETSPDSLVRLPTKESPTNLP
                     STAASDLAISQKSLAALKGAGDAPGIKALKEGAQVPGALKGTGSGAGPYGVPGGSPSG
                     TGIAGGGTGTGTGGGSNTGLKGISNADYSQYLNQLKKRVESVWKYPEEITGVQKVTVR
                     FALDRAGKLTQAVVLESSDSRLNASAVEAMKRASPFPPIPESLKDLANEPLIMRFEVS
                     NRVRG"
     gene            complement(1270..2466)
                     /locus_tag="VFN58_05915"
     CDS             complement(1270..2466)
                     /locus_tag="VFN58_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917560.1"
                     /translation="MNRRLGIIVVGLLVLLAGCAPVAVQQPQVSTQQPQVSTKQPYPD
                     PNPDPNINNPNSPATAQAQPQISGSQRWQHAWGKAMEGLALGGAVGGLYGAGGGLVLG
                     LIAGLVMADSHYGAVNAQIQSEQQKDRNLEAAIEQELERQRALEGQVAQASTPASPST
                     ANPPAVQPVRDAKQPQAPVPQSKPAESKPQENVTVASRTKPPPPPAVAPTPFKNVEVR
                     DINGDGVPDLWIYYNPQKPGEIMRQEEASKGDGRVDTWSYFKDGKLVRREVDTKGHGK
                     PDTSFYYVNDQISREERDESGEGRVSYRAVYENGRLARLEKDADGNGRMNLWVYYDTA
                     KDGEVISKEERDLNGDGVADLWSYYDNGRLVRRDVSAIGLELLSKREQIPATAADPKQ
                     ISAPGS"
BASE COUNT          524 a          727 c          699 g          641 t
ORIGIN      
        1 ttccgtaatg cgcaaggtga cggaacccat gttcttaagc gaccataggc gttccaatac
       61 caccgtgacg ctgattatcg acaacaaaat aagcggataa gtggcgatgg cgcctttgtg
      121 aatgatgtcg ataagatcca gaccctgttg ctgcatgagc gtttccttct ataatcccct
      181 gaagattacc tgaaacgaaa attaattcaa tgaaagatct tgagcgaaag tccgagaaag
      241 ccgatctctt atcctcggac tcggttggaa acctcgaagc gcataatcag aggttcattg
      301 gcgaggtctt tcaaactttc cggaatcggg ggaaaagggg aagcccgttt cattgcttcc
      361 acagcactgg cattcagtct tgaatccgac gactccaaga cgacagcctg agtgagcttt
      421 ccggcgcgat cgagagcaaa acgaacggta actttctgaa ccccggtaat ttcctcggga
      481 tacttccaga ccgactctac ccgcttctta agctggttta ggtactgact gtagtctgcg
      541 ttcgaaatgc cctttagacc cgtattgctg cctccgcccg tacctgtacc ggttccgcct
      601 ccagcgatgc ctgttccact tggacttcct cccggaactc cgtaaggtcc ggcaccactg
      661 ccagtgccct tgagtgcgcc tggaacctgc gcgccctctt tcagcgcttt gattcccggc
      721 gcgtctcctg ctcctttgag tgccgcaaga ctcttttggc tgatcgctag atcactggca
      781 gcggtggatg gcagatttgt gggcgattcc ttagtcggca gtcgaacgag cgaatcgggc
      841 gatgtctctt ttgcattgac agtttcatgt cgggtgtctg tcgctaaggc cttgggcccc
      901 gggaggacca ctgggtttgg cgcaggctca ggttcgacac ttttacttgg aaccgaactc
      961 tccagcgatc cagtgtcgac cttgccagta cgctgcgccg gaggagtagg cgtgcccaat
     1021 ttgggtggcg tgcggggccc gccgggaccc ttaccggggc cgatgctttt ggtatccgag
     1081 ccggtgccgc tgacgtccaa caattgaacc gagatcgggg gttcctgtcg cacgatcgaa
     1141 aaagtagcgg cccgccagac caacaaaaaa agcattgcat gcagcaatag agagggaaaa
     1201 agaaatgggc tgcgaaaaag ggagaaaagg ctgagatctc cccagccact tgacacgtta
     1261 ctggtcatat cagctgcctg gggcggaaat ctgcttgggg tctgcagcag tggccggaat
     1321 ctgctcgcgt ttagacagga gttctaagcc gattgcgctg acatcgcggc gcactaaccg
     1381 gccgttatcg taatagctcc acaggtcggc aacgccatcc ccgttaaggt cgcgttcttc
     1441 tttactgatg acttcgccgt ctttcgccgt atcgtagtac acccaaagat tcatcctgcc
     1501 gttaccgtcg gcatctttct ctaagcgcgc aaggcggcca ttttcgtaaa cggcgcggta
     1561 gctaacccgg ccctcaccgc tttcgtcacg ctcttcacgg gagatttggt cgttgacgta
     1621 atagaaggaa gtgtccggct tcccgtgccc cttggtatcc acctcgcgtc gaaccagttt
     1681 gccatcttta aagtagctcc aggtgtcgac tctgccgtcg cccttggaag cttcttcctg
     1741 acgcataatt tctcccggct tttgaggatt gtaatagatc cataaatcag ggacaccgtc
     1801 gccgttaatg tctcttactt cgacgttttt aaacggtgtc ggcgcaacgg ctgggggcgg
     1861 tggaggttta gtccgggagg cgacggtgac gttttcctgt ggtttgcttt ccgcaggctt
     1921 gctttgaggc actggcgctt gcggctgctt agcgtccctt accggttgca ctgcaggtgg
     1981 atttgccgta ctgggcgagg caggagtcga tgcctgtgcc acttgccctt ccaatgccct
     2041 ttggcgttcc aactcttgct cgatggcggc ttcgagattc cggtccttct gttgttctga
     2101 ttggatctgc gcgttgaccg ctccgtagtg agaatcagcc ataactaaac ctgcgatcaa
     2161 tccaaggacg agtccgccgc ccgcgccgta gagcccgccc acagccccac ctagggccaa
     2221 gccttccatc gctttacccc aagcatgctg ccaccgttgt gagccgctaa tctgaggttg
     2281 agcttgcgcc gtagcgggac tgttagggtt atttatatta gggtcgggat tcggatcggg
     2341 ataaggctgc ttggtggaca cctgaggctg ctgggtggat acctgaggct gctggacagc
     2401 tacgggagcg cagcccgcaa gtaataccag gagtcctact acgatgattc ccaaccgcct
     2461 attcatatcg aatccagctg aaatcttact ttgtatcgct cttggactcc attcccgcct
     2521 tggcagctac ggtgtctccc ggctttgaag actcatagct tgccatattt ctgaactcct
     2581 cttcaaccgc a
//
LOCUS       DASSWZ010000244         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_617455, whole genome shotgun sequence.
ACCESSION   DASSWZ010000244 DASSWZ010000000
VERSION     DASSWZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617455"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFN58_05920"
     CDS             <1..293
                     /locus_tag="VFN58_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917561.1"
                     /translation="LIFTSNTYHHIDNRVSYLTSLRKYLRPNGRIAIIEFDRRGWLDG
                     LWQHYTPSEFIKREMEQAGYKLQNEFNFLDRQSFLIFAPNPPKQGPSAAPSN"
     gene            complement(313..951)
                     /locus_tag="VFN58_05925"
     CDS             complement(313..951)
                     /locus_tag="VFN58_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917562.1"
                     /translation="MSTCSLLVALALIKSHPASAQGSVQEYYDRDYGYVFQYPDSWKL
                     QRLPEGDANRDFRLLLQGTEGSSFMVVVDKTKTTLKKADFENGSERTQRVGAMMQQTI
                     TQIYQSISENIQAANMKVGERSDLSNEFGVKFYIATLNEMKSGKPIIVAGIHAFPFNK
                     DYSINFTMMTFYDPAATQEHQRLTEVFNSFRLIGEPQSGNNPPKPPAAKEPQ"
     gene            1178..3178
                     /locus_tag="VFN58_05930"
     CDS             1178..3178
                     /locus_tag="VFN58_05930"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET8917563.1"
                     /translation="MTKQYILKNTPLSTHRSSRLDYRNELNDAQYQAATTVEGPLLII
                     AGAGTGKTRTLVYRVAHLIDIGVDPRSILLLTFTRRAAEEMLRRASLLIDSRCTQVAG
                     GTFHSFANLVLRQFGRRSGLSPSFTIMDRSDSEDAIQLLRTEMGLNYKDRRFPRKQTV
                     AEIFSMAVNKQTTVPDLLEREYPHLYDSLDDLLQLYERYVDYKATKSLLDYDDLLTKL
                     KDLLSDQEEVRRRLSQIYQFIMVDEYQDTNALQAQIIRLLAATHNNVAVVGDDAQSIY
                     SFRGANFRNIMDFPEAFSGTRIIKLEENYRSTQPILNLTNKIIQLAKERYDKRLFTHK
                     QEGETPVLVQAESEQMQSRFVCQKILELREEGVPLWDIAVLFRSSFHSFDLELELARH
                     NIPFIKRGGFQFMETTHIKDLLAHLRILANPQDTISWNRVLLLLEGVGPQMSQKITGW
                     LLAGSQPVERLRTFQAKAKIAHELKTLAQVLQYASQAERPAEQTQYLMQYYAPILKRS
                     HPDDHPKRLRDLEHFQGITDRYRSLERLLADMALEPPNDSVGGVLAVDPDEGPVILST
                     IHSAKGLEWHSVFIIWALEGRFPSFYNINTDEELEEERRLLYVAATRAKENLFISYPI
                     KIFDRGLRMVLSRPSQFIEGISEDLLEPVTVVDEERNWGWEP"
     gene            3180..>3755
                     /locus_tag="VFN58_05935"
     CDS             3180..>3755
                     /locus_tag="VFN58_05935"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET8917564.1"
                     /translation="MVQFLLNIVVFVSGAVLMSLEIVGSRVLAPYFGNSIFVWGSLIS
                     VVLAALSVGYYWGGWLSAREPSYSRLLILLVIPGVLIFFLPFIYPTVNEWIAGIDFGT
                     RLNPLIASSIYFLLPGVFLGTISPYVIRLAATKLSTVGSTAGTLYAVSTCGSIFGTLL
                     TAFYLIPLMGVSNIIHFLGMILVCLSLVVVPL"
BASE COUNT          809 a         1048 c          974 g          924 t
ORIGIN      
        1 acctcatctt taccagcaat acctatcatc acatcgataa ccgcgtaagc tatctcacca
       61 gcctccggaa gtatctccgc ccaaacggcc ggatcgcgat tatcgaattc gaccgcaggg
      121 gatggcttga cggcttgtgg cagcactaca cgcccagcga attcatcaaa agagagatgg
      181 agcaggccgg atacaaattg caaaacgagt tcaactttct ggaccggcaa tctttcctaa
      241 tttttgcgcc caatccgccg aagcaggggc cgtcggccgc gccgtccaac tgaggcagac
      301 aaccgtcatc ggtcactgcg gttctttagc tgcgggaggc tttggcgggt tgttacctga
      361 ttgcggctcc ccaatcaaac ggaaggagtt aaagacctcg gtgagccttt ggtgctcctg
      421 ggtcgccgcc gggtcgtaaa acgtcatcat ggtgaagtta atggagtagt ccttgttgaa
      481 agggaaggca tgaattccag ccacaatgat gggtttcccg cttttcatct cattgagcgt
      541 cgcaatataa aatttgacac cgaattcatt ggagaggtcg ctgcgctcgc cgaccttcat
      601 gttcgctgcc tggatattct ccgagatgga ttggtagatc tgcgtgatgg tttgctgcat
      661 catcgcgccg acgcgttgtg tccgctcgga accgttttcg aagtcagctt tcttaagggt
      721 tgtttttgtt ttatcgacga ctaccatgaa ggagcttccc tccgtcccct gcagcaagag
      781 ccgaaaatcc ctgttggcat cgccctcggg caaccgttga agtttccaac tgtcaggata
      841 ctgaaacaca tagccgtagt cccggtcata atactcttgc acgctcccct gagcgctcgc
      901 agggtgggat ttgatgagag ccagcgcgac cagcaagctg caagtcgaca accagaagta
      961 tcttgacggg gggcgcgtta gcaccgattt cacgatttca taaggctatc atagatttca
     1021 cgactaacaa taagaaacgt cctgctgcgc cacgattgag tgcagcctgg tcgctccttg
     1081 attgccatcg atattttccg cttggatgaa aacgttgccg ctgatccctt gcccgattcc
     1141 aggaaaatag gtactttagc ttgcaactgg agccccattg acgaaacagt acattctcaa
     1201 aaatacacct ctttcaactc atcgttcttc acgcctcgac tatcgcaacg agcttaacga
     1261 cgctcagtat caagcggcca cgacggtcga ggggccgttg ttgattatcg ccggcgccgg
     1321 caccggcaag acccgcacct tggtttatcg cgttgcgcac ctcatcgata tcggcgtcga
     1381 tccccgttct atcttgctgc tgaccttcac ccgccgggct gccgaggaaa tgctgcggcg
     1441 cgccagccta ctgatcgata gccgctgcac ccaggtcgcc ggaggaacct ttcattcgtt
     1501 cgccaacttg gtgttgcgcc agttcggtcg tcggagcggg ctctctcctt cctttacgat
     1561 catggaccgg tcggatagcg aagacgcgat tcaactgctg cgaaccgaga tgggtctgaa
     1621 ttacaaagac cggcggtttc cacgcaagca gacggtggcg gaaatattca gcatggccgt
     1681 caataaacaa accaccgtgc cggatctgct ggaacgagag tacccccatc tctatgattc
     1741 gctggacgat ctgctccagc tctatgagcg ctacgtcgac tacaaggcaa ctaaatcgct
     1801 gctcgactac gacgaccttt tgaccaagct gaaagatctt ctcagcgacc aggaagaggt
     1861 acgccgccgt ctttcccaga tttaccagtt cattatggtc gacgagtatc aggacaccaa
     1921 tgctcttcag gcgcagatca tccggttgct ggccgccacg cacaataacg tggcggtcgt
     1981 gggagacgac gcgcagagta tctattcatt tcgcggcgcc aattttcgca acatcatgga
     2041 ttttcccgag gcgttttcgg gaacccgcat tatcaaactt gaagaaaact accgcagtac
     2101 ccagcccatt ctgaatttga ccaacaaaat catccagctt gccaaggaac gctacgacaa
     2161 gcgactcttt acccacaagc aagagggtga gacccccgtt ttggtccagg ccgagagcga
     2221 acagatgcag tcccgtttcg tctgtcagaa gattttggag ctccgcgagg aaggcgtgcc
     2281 gctctgggat atcgccgtcc tgtttcgatc gagcttccat tccttcgact tggaactgga
     2341 gctggcgcgg cacaatattc cgtttatcaa gcgcggcggt ttccagttca tggaaactac
     2401 tcatatcaag gatctcttgg cccatttgcg catcctcgcc aatccacagg atacgatttc
     2461 gtggaaccgg gttttgctgc tcttggaggg agtcgggccg caaatgagcc agaagatcac
     2521 cggctggctc ttggcaggaa gccaacctgt ggagcgtttg cgcacctttc aagccaaagc
     2581 caagattgcc cacgaactca aaaccttggc ccaggtctta caatacgcgt cgcaggccga
     2641 acggcccgcg gaacagacgc agtatttgat gcagtactac gcgcctatcc ttaagcgcag
     2701 ccacccggac gatcatccga agcggcttcg ggatctggag cactttcagg gaatcaccga
     2761 ccgatatcgc agtctagaac gtttgttggc cgacatggct ttggaacccc ccaacgatag
     2821 cgtcggaggc gtcttggccg tggacccgga cgagggtccg gtgatccttt cgacgatcca
     2881 ttctgcgaag gggttggaat ggcattccgt cttcatcatt tgggctttgg agggaaggtt
     2941 cccctccttt tacaatatca acacggacga ggaactcgaa gaagagcgcc ggctcttgta
     3001 tgtcgcggcc acgcgggcta aagaaaatct tttcattagt tacccgatca agatcttcga
     3061 ccgcggacta cgcatggtct tgtctcgtcc ctctcaattc atcgagggta tttccgaaga
     3121 cctgctcgag ccggtcacgg ttgtcgatga ggaaagaaat tggggttggg agccttaaca
     3181 tggtgcaatt tctgctcaat atcgtcgtgt tcgtgagtgg ggccgtattg atgagtcttg
     3241 aaatcgtcgg cagccgcgtg ctggcccctt acttcggcaa ctcgattttc gtttggggaa
     3301 gcctgatctc tgtcgttctg gccgcattga gcgtcggcta ctactggggc gggtggcttt
     3361 cagcccgcga accttcctat tcccggcttc tgatattact ggtgattccg ggcgttctga
     3421 ttttttttct gccctttatc tacccgactg ttaatgagtg gatcgccggt atcgatttcg
     3481 gtacccggct gaaccccttg atcgcgagca gcatctactt cctgctgccg ggagtttttt
     3541 tgggaaccat ctcgccctac gtcatacgtt tggcggcgac caaactttcc actgtcggca
     3601 gcacggctgg aaccctctat gccgtttcta cctgtggcag tatctttggc acccttttga
     3661 ctgcttttta cttgataccg ctcatgggtg tcagtaatat catccacttt ctgggtatga
     3721 tattggtctg cctttcactg gtcgtggtgc cgctg
//
LOCUS       DASSWZ010000245         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_518447, whole genome shotgun sequence.
ACCESSION   DASSWZ010000245 DASSWZ010000000
VERSION     DASSWZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518447"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFN58_05940"
     CDS             <1..553
                     /locus_tag="VFN58_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8917565.1"
                     /translation="IGSGIIVSLATIFPEAGIEVSSLIYGRDAPRGVPLDQARKAEKP
                     AFTPVPPGSYTSAAIILLTDGQRTTGPDSIEAARMAADRGVRVFTVGIGTTSGETIGF
                     EGWSMRVRLDEETLKAIANMTRGEYFYAGTATDLKKVYQSLNAKLVLEKKDMEISALF
                     SAAAAVIALVSALLSLLWFNRIL"
     gene            complement(686..1714)
                     /locus_tag="VFN58_05945"
     CDS             complement(686..1714)
                     /locus_tag="VFN58_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8917566.1"
                     /translation="MKNFILALLLFLASSSNLHAQTPFYQGKTVRIIVGNLAGDAYDL
                     WARIFAQHMGKYIPGNPNFIVQNMTGAGGVTAANYVYAVAKPDGLTMGTFGPSMYFDQ
                     LAGRKEVQFDWSKFTWIGTPEQTEFILIMRTDNPYKSIEDIRKAAVPPKCGATGTSTS
                     GYYIPKFLDEGMGIKFNIVTGYPGGGEIDLGIERGELHCRNLTISTYFAREPFLTWHK
                     NGFVRPIMQTPKKRDARVPDVPTIYELMDQYKTSETNRRVATVLLAPAIFGRPTVATP
                     GVPPDRVKILRDAYHTSLKDPALLEDLKKRRWEVDAISGEEMANLAKEVISQPPEVIE
                     RMKQLLGN"
     gene            2070..2372
                     /locus_tag="VFN58_05950"
     CDS             2070..2372
                     /locus_tag="VFN58_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET8917567.1"
                     /translation="MGNKLYVGGLPYSVTDGRLQEIFSVHGTVESANVISDKFTGQSR
                     GFGFVEMSSGGEAQKAIEALNGTQLDGRTLTVNEAKPMARRDNDGGGRRDSGRNRW"
     gene            2616..2984
                     /locus_tag="VFN58_05955"
     CDS             2616..2984
                     /locus_tag="VFN58_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917568.1"
                     /translation="MIEREVVKQTAELLKGIIRSIDKKLEYSLVDQTQEGRFSLRLSS
                     RGREGIVSLLTEDLRLAVLDNVRKNAIRQKIKSTRDHLLSNYVVDVMGKKVAKMLKQA
                     NGAKDEYKPSFFRPSQGRRR"
     gene            complement(3001..>3225)
                     /locus_tag="VFN58_05960"
     CDS             complement(3001..>3225)
                     /locus_tag="VFN58_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006091143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET8917569.1"
                     /translation="PAGFTREVYDRVPDEKDPNGSRLEGPKQVQLPVGLEFLGRPFEE
                     AKLFEIASAYENVKQHRRPPAGFGRLPREP"
BASE COUNT          736 a          872 c          864 g          753 t
ORIGIN      
        1 tattggcagc ggcatcatcg tctccctggc gacgatcttc ccggaggcag gaatcgaagt
       61 gagttcgttg atctatgggc gtgacgctcc gcgcggcgtt ccgctcgatc aggctcgcaa
      121 agcggaaaaa ccagcgttta ctccggttcc gccgggctcc tacacatccg ccgccatcat
      181 cttgctcacc gatggccagc ggaccacggg ccccgactcc atcgaggcgg cccgcatggc
      241 cgccgaccgc ggtgttcgcg tcttcaccgt cggaatcggc accaccagcg gagagaccat
      301 cggctttgaa ggctggtcca tgcgcgtgcg cctcgatgaa gagacgctta aggccatcgc
      361 caacatgacg cgcggagagt atttctacgc cgggaccgca acggacctga agaaggtcta
      421 ccagtccctg aacgccaagc ttgtgctgga aaaaaaggac atggaaatca gcgccctgtt
      481 ttccgcagcg gcggccgtca tcgcgctggt ttcggcgttg ctttcactcc tgtggttcaa
      541 ccggatccta tagccgccca gagcggagct cgcgatagaa agaccccttc cagttacgca
      601 tactaatctc gataggagga acaaaaaggt atcttcgtct tcgacggaaa gtcttctatc
      661 aatccaagtg gctaagagac atgcgctaat ttcctaacag ttgcttcatc cgctcaatga
      721 cctctggcgg ctgggaaatc acctctttcg ccagattggc catctcttcc ccgctgatcg
      781 catccacctc ccatcgtctc ttctttaggt cttccagcaa cgctggatct ttgagcgatg
      841 tgtgataggc gtcacgcaag atttttaccc tatccggcgg aacacccggc gtggccaccg
      901 tgggtctgcc aaaaatcgcc ggagcgagca gaaccgtggc cacacggcgg tttgtctcag
      961 acgtcttgta ttggtccatc aactcataaa tggtaggaac atcgggaact cgtgcatctc
     1021 gcttctttgg cgtttgcatg atcgggcgaa caaagccgtt cttgtgccag gttaagaagg
     1081 gctcgcgcgc gaaataggtg ctgatcgtca agttgcggca gtgcaactcg ccgcgctcga
     1141 ttccgaggtc aatctccccg ccgcctgggt aaccggtgac gatgttgaat ttgatcccca
     1201 taccttcgtc caagaacttc gggatgtaat agcccgaagt gctcgttccc gtggcaccgc
     1261 acttgggcgg caccgcggct ttgcggatat cctcgatgga tttgtacgga ttatccgtcc
     1321 tcatgattaa aataaactcg gtttgctccg gcgtgcctat ccaggtgaac ttggaccagt
     1381 caaactgaac ttcttttcgc cctgcaagtt gatcaaaata catcgaagga ccgaatgtcc
     1441 ccatcgtcag accatcgggt ttcgcaaccg cataaacata attggcggcg gtcacgcctc
     1501 ctgctccggt catgttctgc acaatgaaat tgggatttcc cgggatgtac ttgcccatgt
     1561 gttgggcgaa gattctcgcc caaagatcat aggcatctcc cgcaaggttg ccaacgatga
     1621 tcctgacggt ttttccttga tagaaaggag tctgagcgtg gagattggaa ctcgacgcca
     1681 gaaacaacaa cagcgcaagg atgaaatttt tcatatcatc ttcagaccgc ctagacccag
     1741 gcgttagaac cttatcactc ccgttcggtt gttcgctacc tctttctcga atctctgttc
     1801 cggtgcggct tcccaataat aaagcgctgg tgggtggttg acatcatcgc tattaacacg
     1861 tacagtataa ttcctacttc agtatcaggc cttctgaaag aggaagacac aaacgttgaa
     1921 ggcctccaaa tcgtctctcc ttgcgaaacg atctctaaag gacaatccga ccagagcgtg
     1981 gttctagcaa ttcagttacc catgttctgc ccgttagcag tctagttctc agggtcaacg
     2041 acacaaacat tctcacatag gaggagaaca tgggtaataa attatatgtt ggcggtctgc
     2101 cttattcagt aaccgatggg cgactgcagg aaatcttttc agtgcacggg accgtggaat
     2161 cggccaacgt gatatccgat aagtttacgg gacagtcacg cggcttcggc ttcgtagaga
     2221 tgagttcagg gggcgaggcg caaaaagcca tcgaggccct gaacggcacg cagctcgacg
     2281 gccgcacttt gaccgtcaat gaagccaagc ctatggcacg gcgggataat gacggtggcg
     2341 gacgccgcga cagcggacgt aaccgctggt agtctttttg ttagatagct aatcaccttg
     2401 ggggcaatcg gtcgggttgc ccctcttttc ttgcaaaaac gagtcggctt ggtgccgctt
     2461 cgccggggta gggtttgttg cttccgtgat ccttgcgttg gctttctgtc gggcacgccg
     2521 gaaccgagtt gttattgtcc caagttttct taagcccgag gacattcggc ggcctcagag
     2581 ctttttatga gccgtaaaac agagggggaa attccatgat tgaacgtgaa gtggttaaac
     2641 agacggcaga gttattaaaa ggtattatcc gcagcataga caaaaagctt gagtacagtt
     2701 tggtggacca gactcaagaa gggaggttct ctcttcggct gtcatcacgc gggcgggaag
     2761 gaattgtttc cttactcaca gaggacttgc gactcgccgt gttggataac gtgcgcaaga
     2821 atgcgatccg gcaaaagatc aagagcacac gcgaccattt gttatccaac tatgtcgtgg
     2881 atgtcatggg aaaaaaggtt gcgaagatgt tgaaacaggc caacggggcg aaggacgaat
     2941 acaagccgtc tttctttaga ccctcgcagg gaagaagaag ataacacttg ccatttcatt
     3001 ttacggctcc cgcgggaggc ggccaaatcc tgcgggcggc cggcgatgtt gtttgacatt
     3061 ttcataggcg gacgcgatct cgaacagctt tgcctcctca aacgggcgcc caaggaactc
     3121 caacccgacc ggaagctgaa cttgctttgg cccttctaac cggctgccgt tgggatcttt
     3181 ttcgtccggc acgcggtcat agacttccct ggtaaagccg gcagg
//
LOCUS       DASSWZ010000246         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1325057, whole genome shotgun sequence.
ACCESSION   DASSWZ010000246 DASSWZ010000000
VERSION     DASSWZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325057"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFN58_05965"
     CDS             <1..327
                     /locus_tag="VFN58_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917570.1"
                     /translation="DKGCPLGMNFTNPAPASVGSHRGIAKGTKNPHAAALYMDYVLSA
                     EGSAVLAKAGGLPARKGAKAVYEEVSNLEEKGVRMLMMGYQDMPKVKEKGYKLIEEII
                     IRKQLR"
     gene            complement(333..2063)
                     /locus_tag="VFN58_05970"
     CDS             complement(333..2063)
                     /locus_tag="VFN58_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8917571.1"
                     /translation="MDDVIEKLAIKNPIRFRINPQFILVGLAIALVAYLTLVPLLMLI
                     YGSLQSGQPGEPADFTLKNYLTLLGKWRLVPAFLNSLIFSIGASFLTFAGGLYLAWMT
                     ERTNIPWKGALYAMVLVPMVVPGLLTTVGWIILFSRRTGIVNIVATQYLGFEQPLEIN
                     NMLGMIWVLGVDQIPLAFLLLAAALRSMDPSLEEAAMVAGSGIVRTTLRITLRVLLPA
                     ILAVWMITFVRAIENFEVPALIGMPAGILVFSTEVYLATKTTPTDFGLASTFGIVYLA
                     ITAVGIFFYLRLTAMSERFATITGKGYRPSPINLGRWRVALAALNILFCFVVFFLPIL
                     IVVWSSFLPFYMPPSAKALASLTLDNYTRLWEFPLIQRAMWNSFVLGVSSSTITMLLT
                     AIVAWIIVRTSWRGKGVLDFLAFSPIAVPGLVMGIAILWLYLVAPVPVYGTLWILLIA
                     YVTKYLPYGMRACSSSMLQIKKELEEASEVSGATWPRTFSRVILPLLAPGFVAGWVFV
                     VTHSFRELATSIMLYRSGTEVISVVLFELWETGQYPQLSALGMILVGILIVISLMARA
                     IGGKFSIQQV"
     gene            complement(2197..2622)
                     /locus_tag="VFN58_05975"
     CDS             complement(2197..2622)
                     /locus_tag="VFN58_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8917572.1"
                     /translation="MQLLDVFEMAKGRKKGQRRMDLFRRENLETWILTFPPGDVQRMH
                     NHPSDRTYYVLTGRGIMKGLNETHEVSAGQIISIPAHEYYEGSNPYDEPMILLGNSHK
                     ATPEDVAKAGGQRNEIDEKTGKRIIYQHGGGQDQGVLAD"
     gene            2832..3731
                     /locus_tag="VFN58_05980"
     CDS             2832..3731
                     /locus_tag="VFN58_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917573.1"
                     /translation="MSLVQLNIMYGHLEGIEGKFARDPSGYLSIEAGIYQKHGLDVSW
                     NHVQGTEERYRRLEDGTAQISLVVGRASLQHFLDSKTTRILGCVMNSCPYYLVASPAI
                     RQIGDLKGKTIVCREGPSRHTPIAETFRERAHLSIGKDLMLELPNSDQEAFQLLVSGK
                     VSAALLPRPYGFWAEERGFKRITDWPDVVDDPLPISIETTATLLRERGKDFSTFLQAH
                     REGIRFMKTHRGETIRVLQEKFGHSPSFAGKTFDDYLICVDEELTVDFKHLEKLLSQV
                     APETQGGARQVASEWIVPGGLKG"
     gene            3861..>4285
                     /locus_tag="VFN58_05985"
     CDS             3861..>4285
                     /locus_tag="VFN58_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917574.1"
                     /translation="MAGIIDSDTHIAESEAMWSMMDKDMYDRRPVLLKAPEDTLYGPR
                     NAFWLIGGDIFPKPNGKGSFRLITPSATKLESARGDIAIGCREMTDISSRLNDMDRLQ
                     VDTQVVFPTLFLIYITDDVALDIAMARAYNRFIAQACAKG"
BASE COUNT         1092 a         1105 c         1178 g          910 t
ORIGIN      
        1 gacaaggggt gccctctcgg gatgaatttt accaatccgg cgcccgcctc tgtggggagc
       61 caccggggga tcgccaaggg cactaagaat ccgcacgctg cggcgcttta tatggattat
      121 gtcctaagcg cggaagggtc cgcggtgctg gccaaggcgg gcgggctgcc agccagaaaa
      181 ggagccaaag ctgtctatga agaggtctcg aacctggagg aaaaaggggt gcgcatgctc
      241 atgatgggct atcaagacat gcccaaggta aaggagaaag gttataaact gatcgaagag
      301 atcatcattc gaaaacagct ccgctgaaga aactatacct gttgaataga aaactttccg
      361 ccgatcgcgc gcgccatcag gctgatcact ataagaatgc caaccaggat catgcctaag
      421 gccgaaagct gggggtattg tccggtttcc cacagctcaa agagcacaac cgagatcact
      481 tcggtcccgc ttcggtaaag cataatcgac gtggctagct cgcgaaacga atgggtcacg
      541 acaaaaaccc atcccgcgac aaaacccggc gccaaaagcg gaaggatcac tcgtgaaaaa
      601 gttctcggcc aagtggcccc gctaacctcc gaggcttctt ccaactcctt cttaatctgg
      661 agcatcgaag acgagcacgc cctcatgccg tagggaagat actttgtaac gtaggcgatc
      721 aagagaatcc aaagcgttcc gtaaaccggc accggcgcta ccagatagag ccaaaggatc
      781 gcgataccca taaccagacc aggaacggcg atcgggctga aagccagaaa gtcgagcacg
      841 cctttgccgc gccaggaggt ccgaactatg atccaggcca cgatcgccgt gagcagcatg
      901 gtgatggtcg agctggagac gccgaggacg aagctgttcc acatggcgcg ttgaatcagc
      961 ggaaattccc aaagccttgt gtaattatcc aaggtgaggc tggccaatgc cttcgctgaa
     1021 gggggcatgt aaaagggcag aaacgaagac caaacaacga tcagaatggg cagaaaaaag
     1081 accacaaaac agaacagaat atttaaggct gctaacgcaa cgcgccaacg accaaggtta
     1141 ataggcgagg gacggtagcc cttgccggtg atggtggcaa agcgttccga catggcagtt
     1201 aatctcagat aaaagaagat gcccaccgcc gtgattgcga ggtaaacgat accgaaggtg
     1261 cttgccaggc caaagtcggt gggtgtggtc ttggttgcca gatagacctc tgtcgagaaa
     1321 accaaaattc cagcgggcat tccaatcaac gccggcactt caaagttttc aatcgccctg
     1381 acaaaagtga tcatccaaac cgcgaggata gccggcaaaa ggacgcgcaa ggtaatgcgc
     1441 aacgtggtgc gcacgatacc ggaaccggca accatcgctg cttcttccag agacgggtcc
     1501 atggaacgca aggcggcggc aagcaaaaga aaggcaagcg gaatttggtc cacccctaag
     1561 acccagatca ttcccaacat gttgttgatt tccaggggtt gctcaaatcc tagatattgg
     1621 gtcgcaacaa tattgactat tcccgtccgc cggctaaaca agatgatcca acccaccgta
     1681 gtgagaagcc cgggaacgac catcggaacc aaaaccatcg cgtagagcgc gcccttccag
     1741 ggaatgttgg ttctttccgt catccaggca aggtaaagcc cgccggcgaa agtgagaaag
     1801 ctcgcgccga tgctgaaaat gagagaatta agaaatgctg gaaccaggcg ccatttgcca
     1861 aggagcgtga gatagttttt caacgtgaaa tccgccggct ctcctggctg tccgctttgc
     1921 aggctgccgt agatcagcat cagcagcggc acgagggtga gataggccac cagggcgatt
     1981 gccaatccca ctaagataaa ctggggatta atgcggaacc gaatcgggtt cttgatcgcg
     2041 agcttttcta tgacgtcatc catagaaacc acaactgact tattctggct tcagaaaagc
     2101 ggctcttctt gacgatcggt ggtcaataca cgaaagcccg gccgggttgc ccccaggccg
     2161 ggcttgctta aaacaaaacg aagcggagtt cgaatgctaa tctgccaaca ctccctggtc
     2221 ttgaccccca ccgtgttgat agatgattcg cttgccggtc ttttcgtcga tctcgtttcg
     2281 ctgaccaccg gccttggcca cgtcttccgg cgtggctttg tggctgttgc ccaagagaat
     2341 catcggctca tcgtagggat tcgatccttc ataatactcg tgggccggaa tgcttatgat
     2401 ctgcccggcc gagacttcat gagtttcgtt aagacccttc atgattcccc gcccagtgag
     2461 cacgtagtaa gtgcggtccg aaggatggtt gtgcattctc tgaacatcgc cgggaggaaa
     2521 ggtgagaatc caggtctcga gattctccct cctgaacaga tccatgcgtc gctgtccttt
     2581 ctttctccct ttggccattt cgaaaacatc caacagctgc atattgacct ccttttttgt
     2641 tacgtgacga gtttcgtcga acttggcctg actttattcc cgcaagagag agcttgtcaa
     2701 ggagcgagac gtgtcttccg cagagaatcg tcataggtcc tgtaagcaaa tcaatggtcc
     2761 ggatcgcatt tgtgtgtagg atggaggcgt aatatagaaa cggttgagta ttggttgggg
     2821 aggaatcgta tatgagcctg gttcaactca acataatgta tggccacctc gagggaattg
     2881 aaggtaagtt tgcgcgagat ccttcaggtt atctctccat cgaggctggt atctatcaga
     2941 agcatgggct ggacgtatcg tggaatcatg ttcagggaac ggaggagcgt tatcgcaggc
     3001 ttgaggacgg caccgcgcag atctcgcttg tcgtcgggcg agcttccctg cagcactttc
     3061 tggattccaa aactaccagg attctcggct gcgtgatgaa tagttgccct tactaccttg
     3121 ttgccagtcc ggcgattcga cagatcggtg acctcaaggg aaagacgatt gtatgtcgag
     3181 aaggcccttc ccgacatacg ccgatagcgg agactttccg agagcgcgcg cacttaagca
     3241 ttgggaaaga tctcatgctg gaattgccaa acagcgatca ggaggctttc caactcttgg
     3301 ttagcggaaa agtaagtgcg gcgttgctcc cgcggcccta tggcttctgg gcggaagaaa
     3361 gaggattcaa aagaatcacc gactggcccg atgtcgtcga tgacccgctg ccgatttcga
     3421 tcgaaacgac cgcaaccctg ctgagggagc ggggaaagga tttttcgaca tttttgcaag
     3481 cccatcgcga ggggattcgt tttatgaaaa ctcatcgcgg tgagaccatc cgcgtgctac
     3541 aggaaaaatt tggtcactcc ccgtcatttg ccggcaagac ctttgacgat tatctcattt
     3601 gcgtggacga ggaattgacg gtggatttca aacatttgga gaaacttctt tctcaggtgg
     3661 ctccggagac acagggcggc gcccgacaag tggcgtcaga gtggatcgtg cccgggggtc
     3721 ttaaggggta gcatgtggct gcaaccgaca gccgtctggc gactaccccc aaaagcagtt
     3781 tcttgctata tctcgacacg aaggcaccgt ggtagtagtg ataacagaag taaacagagt
     3841 ctaacgagcg gaggtgtttt atggcaggta taattgattc ggatactcat attgcagaat
     3901 ccgaggcgat gtggagcatg atggacaaag acatgtacga ccggcggccg gtgttgctaa
     3961 aagcgcctga agatacgctt tatggtcctc gcaacgcctt ttggctaatc ggcggcgaca
     4021 tctttcccaa gcccaacggc aaaggtagtt ttcgtctcat cacgccgtct gcgaccaagc
     4081 tcgaatctgc ccgcggcgat atcgccatcg gctgccggga aatgaccgat atttccagcc
     4141 ggctgaacga catggacagg cttcaagtcg atacgcaggt cgtctttccg accttatttt
     4201 tgatctatat caccgacgat gtagctttag acatcgcgat ggctcgcgcc tacaaccgct
     4261 tcatcgccca ggcctgcgcc aaggg
//
LOCUS       DASSWZ010000247         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_900711, whole genome shotgun sequence.
ACCESSION   DASSWZ010000247 DASSWZ010000000
VERSION     DASSWZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900711"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..817)
                     /locus_tag="VFN58_05990"
     CDS             complement(56..817)
                     /locus_tag="VFN58_05990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET8917575.1"
                     /translation="MKPFVFALLLTILAGCSAPKGDVTWRVREMALAPTTKVVLYGPG
                     EKVLLTMNRQTVQKLLLAHFRITRSAGIQAELFIVEGEDPNAFAASIDGRRIIAINVA
                     MVKLIGENMDEFAALLGHEAAHWAKGHVDSSKARSNTIQGISTVVGVGLGVAGIPAAG
                     VITGLSANLIEASYSRDNEREADALSIDFMAVNGFDPQGAVRLQEKMLRLPAGLKVPF
                     LSTHPSGKERVENLKKLIAAKQSQPRAEQSDGENR"
     gene            931..1650
                     /locus_tag="VFN58_05995"
     CDS             931..1650
                     /locus_tag="VFN58_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET8917576.1"
                     /translation="MRESIALLIMLAIFPSLASAQQSEKRPRPPSVTANGEAVITVEP
                     DQAQIDIGVVTQARNAPEAAKENAEKLARVMSEVKKLLGKGDEVKTASYSLNPNYRYP
                     QGGKPEIVGYTATNVLHIKTGALDNVGKLIDAAMQSGANTIQRLLFTLKDEHSAQLQA
                     LRLASIKAKAKAEEMANALGLKIVRVLSVTEGERGVRPIVMPQPRVTQMEAAAAAPTP
                     VEAGTIEVRSSVSLTAEVGPK"
     gene            complement(1810..>2717)
                     /locus_tag="VFN58_06000"
     CDS             complement(1810..>2717)
                     /locus_tag="VFN58_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET8917577.1"
                     /translation="FCEIKRSKQMKNPRYDYLPIIDRKKIVWPNDGRIALWVAPNIEY
                     FHFDMPIRGSGSNHAPDVPGYTLRDYGSRVGVFRIMEVLDKYGIRASVLLNAEVCEQH
                     PPIIEEGNKRQWEWLGHGMTNSISMLDYPVEEERNVIRKVKEIITSATGKAPKGWLGP
                     GLGETFNTPDHLAAEGFEYVCDWGGDEQPVPLRVKSGRMIVVPYELGVNDIRVFIRES
                     HTPEQYYRMVCDHFDTLYRDSVTGGRVLCLPLHPFVIGMPYRIKYLEKALDYICSHDG
                     VWRTTGWEIAEWYYRHYYVDPGRME"
BASE COUNT          625 a          803 c          673 g          616 t
ORIGIN      
        1 tgacatgcca aaacaattca tcatttatta ctcggtcatt ccaatattcc agtccttagc
       61 ggttttcgcc gtcgctctgt tccgccctag gttgggactg cttcgctgca atcagcttct
      121 tgagattctc aactctctcc ttgccgcttg gatgggtgct caagaaaggc acctttagcc
      181 cggccggcag cctaagcatc ttttcctgaa gccgcaccgc accctgagga tcaaatccgt
      241 tcactgccat aaagtcgatg ctcagcgcat cggcctcgcg ttcgttgtcc cgactatagg
      301 aagcttcgat taaattagcg ctcagtccgg tgatcacgcc tgcggccgga atgccggcca
      361 ctccgagacc gacgccaacc acagtactga ttccttggat cgtatttgag cgtgctttgc
      421 tcgaatccac gtgtcctttt gcccagtgcg ccgcctcatg gcccagcagg gcagcgaact
      481 cgtccatatt ctcgccgatg agtttaacca tcgcgacatt gatggcgatg atacgccggc
      541 cgtctatcga tgccgcaaaa gcattcggat cttcgccttc gacaataaac agttccgcct
      601 gtattccggc gctgcgagtg atgcgaaaat gcgccaggag cagcttttgc acggtctgcc
      661 tattcatcgt tagaaggact ttttccccgg gaccataaag aaccaccttg gtggtcggcg
      721 ccagagccat ctctcgcaca cgccaggtga catcaccttt gggcgccgag caaccggcta
      781 gaatcgtcaa cagcagcgca aagacaaacg gtttcattgg aacaatttta aatgctttac
      841 agtcttatag aagagagccg tcaagactgg cgacggtcac tcttttgctg tagcttttta
      901 agtaggagtc attgtagcag cggaggccat atgagggaat cgattgctct gttgatcatg
      961 ctggccattt ttccgagtct cgcttcagcc cagcaaagcg agaaacgacc ccgcccaccg
     1021 tcagtcacgg ccaatggcga agccgtcata accgtggaac cggaccaggc gcagatcgac
     1081 atcggggtcg ttacccaggc gagaaatgcc cccgaagcgg cgaaagagaa tgccgagaaa
     1141 ctcgcgcgcg tgatgagtga agtcaaaaag cttcttggca agggtgacga ggtcaaaacc
     1201 gccagttact cgctcaatcc caactatcgt tatccccagg gcggcaaacc ggaaatcgtc
     1261 ggctacacgg cgaccaatgt gcttcacatc aaaaccggcg ctttggacaa cgtcggcaag
     1321 ctcatcgacg ccgccatgca gtctggcgcg aatacgattc aacggctgct ctttactctc
     1381 aaagacgaac acagcgccca actccaggct ctgcgtctcg cgtcgatcaa ggccaaagca
     1441 aaagccgagg aaatggccaa tgccctcggc ctcaaaatcg tcagggtcct ctccgttacc
     1501 gaaggtgaac gcggagttcg gccgatcgtt atgccccagc cgcgagtgac acaaatggaa
     1561 gctgcggccg cggcgccgac acccgtggag gcgggcacga tcgaagtgcg gtcttccgtt
     1621 tcactaaccg cagaggtcgg accgaaataa ttcgctcgac agttttccca acacaaattg
     1681 cttctctttg tctttggtca tcgtcaacaa ggttcgtgaa tttgtttcac tggagtaatg
     1741 gagtatcggg atacaatcta aatagaccca ttactccact gctccaccac tccattactc
     1801 cattctgggt tactccattc gccccggatc cacgtaatag tgtcgatagt accactccgc
     1861 aatttcccaa cccgtcgttc gccagacgcc atcgtgagaa cagatgtaat ccaaagcctt
     1921 ctccagatac ttgatccgat agggcatgcc gatcacaaac ggatgaagcg gaagacaaag
     1981 cactctgcca cctgtcacgc tgtcccgata gagtgtatcg aaatgatcgc agaccatccg
     2041 gtagtattgc tccggcgtgt ggctctcgcg gatgaagacc cggatatcgt tgacacccaa
     2101 ctcgtacggt acaacgatca tgcgaccgct cttcacgcgc aacggcaccg gctgctcgtc
     2161 tccaccccaa tcgcaaacat actcgaagcc ttccgccgcg aggtgatccg gagtgttaaa
     2221 ggtctcacct aagcccggcc cgagccaacc cttcggcgcc ttgcccgtcg ccgaggtaat
     2281 aatttccttg actttccgaa tgacgtttcg ctcttcttcc accggataat cgagcatcga
     2341 aatgctattg gtcatgccgt ggcctagcca ttcccactgc cgcttgttgc cttcttcgat
     2401 aatcggggga tgctgttcgc aaacctcggc gttgagaagc acgctggcgc gaatgccgta
     2461 tttgtcgagt acctccataa tgcgaaaaac gccgactctc gatccatagt cgcgtagtgt
     2521 gtagccgggc acgtccggcg cgtgattgct tcccgagccg cggatcggca tgtcgaaatg
     2581 aaaatattcg atattgggcg cgacccaaag cgcgatgcgc ccgtcattgg gccagacaat
     2641 tttcttgcgg tcaatgatgg gaagataatc gtagcgcgga tttttcatct gcttgctcct
     2701 ctttatttcg caaaatc
//
LOCUS       DASSWZ010000248         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1382109, whole genome shotgun sequence.
ACCESSION   DASSWZ010000248 DASSWZ010000000
VERSION     DASSWZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382109"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1362
                     /locus_tag="VFN58_06005"
     CDS             202..1362
                     /locus_tag="VFN58_06005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET8917578.1"
                     /translation="MEKAVAETKLRILREAIQSNVGVVRSSKVKPQGLPKRAAPLWFW
                     GFLLIAIGAAAADFVVRKQEREEVKKMVGVQLDTLHGIQTQKSLAHLAVPPLVVSKGK
                     DPSSHSAAVDMANPLLPVGSHLLNAATPLSALYQMNRTALPLSVRRIVIDPGHGGKQH
                     GAISESGVSEKDITLDIALRLRRLMENGSLEAFMTREADETLPLEKRVAFANANHADL
                     FVSIHVNWMEPREIRPLETYYVGATNDPAVIKLASIENRDSGYSLSDYRRVLDKIYLD
                     TRREESHRLAKAINSELYHSLSLINPELENRGVKMAPFAVLIGTQMPAVLAEVSCLSN
                     EDDVKLLTNADYREKIAQALLRGIVSYANNLNGSEKKVAELWKKPTKPSASE"
     gene            1326..>2176
                     /locus_tag="VFN58_06010"
     CDS             1326..>2176
                     /locus_tag="VFN58_06010"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET8917579.1"
                     /translation="MEETNETLCIGIDLGTSRSSISASNGERHVVDSYVGWPLDMVAR
                     KLVKKTVLVGREALDARSMLDLRRPLERGVIKDGSAKDDEAVQELLRHLISLIGIKHK
                     SKTRGKVRAVVGVPAEAFRVSRQRLRSAMDGVADSVMLVSEPFAVAYGLEALLHAMII
                     DIGAGTADFCVMQGRYPTEADQRTLPNAGDSIDERLLKLMQDHHPEAQVSIHMIRNWK
                     EKWSFVGEPKSRVTVTVPAKGKATEFDITDEMRAACESILAPISETLIDLLSRVEPEY
                     QEQVRNN"
BASE COUNT          526 a          589 c          597 g          464 t
ORIGIN      
        1 ccaaccaccc tagctgtcag ccgattgcgc gcaaagagaa ctataagaga taagggcttc
       61 gacaaacatg acagccgcct gcctttgctc ataagggcct cgcaaatgcg cctttatttt
      121 tcccccgacg atagcgatta acatgacaaa tcaggtagtt tccaatcctg cgctagcatc
      181 ggcaaataac cgccaagtcc aatggaaaaa gctgtagccg aaactaaact ccgcatcctt
      241 cgtgaggcga tccagagcaa tgtgggagtg gtacggagta gtaaagtgaa accccaggga
      301 ctgcccaaac gcgcagctcc tctctggttc tggggctttt tactgatcgc gattggcgcg
      361 gcggcggcag actttgtcgt ccgtaagcag gaacgggaag aggtcaagaa gatggtcggg
      421 gtccaattag acacgttgca tgggatccag acccaaaaga gtctcgcgca tctcgcggta
      481 cccccgctgg tggtttcgaa ggggaaagac ccgtcatctc attctgctgc cgtagacatg
      541 gcgaacccat tgttgccggt tgggagccat ctgcttaacg ccgctacccc tctctcggct
      601 ctatatcaga tgaatcggac agcccttcct ctttctgtca ggagaatcgt gatcgatccg
      661 gggcacggcg gtaaacaaca tggcgccata tccgagtccg gagtctcaga gaaagatatt
      721 accttggata tcgcactgcg actgcgccgt ctcatggaaa acggttccct cgaagcgttc
      781 atgacacgag aggcggatga aacgctgccg cttgagaaaa gagtggcttt tgccaacgcc
      841 aaccatgcgg acctttttgt ttccatccat gtaaactgga tggaacctcg tgaaatccgc
      901 ccgctggaaa cttactacgt cggcgctacc aacgacccgg cggtgatcaa actcgccagc
      961 attgaaaacc gggattccgg ctactctttg tcggactatc ggcgcgtgct ggacaagatc
     1021 tatctcgaca cgcgccgtga agagtcccat cggctcgcca aagccattaa cagcgaactt
     1081 taccactccc tcagcctgat taatcctgag ttggagaatc gtggcgtcaa aatggcgccg
     1141 tttgcggtcc tgatcggcac ccagatgccc gccgtcttgg ccgaggtttc ctgtctatcg
     1201 aacgaagatg acgttaaatt attaaccaat gctgactatc gagaaaagat tgcgcaggca
     1261 cttttgagag gcattgtttc ttatgcaaac aatctcaatg gttcggaaaa aaaggtagct
     1321 gagttatgga agaaaccaac gaaaccctct gcatcggaat agatctaggt acttctcgaa
     1381 gctccatctc cgcctccaac ggcgagcgtc acgtggttga cagctacgtg ggctggccac
     1441 tggatatggt agcgcgcaaa ctggtgaaaa aaacggtcct cgtcggccgc gaagcactcg
     1501 acgctcgttc gatgttggat ctgcgccggc cgctcgaacg gggagtcatc aaagacgggt
     1561 cggcgaagga cgacgaggcg gttcaggaat tactgcgcca tttgatttcc ttgatcggca
     1621 tcaagcacaa aagcaaaacc cgcgggaagg tgcgcgccgt cgtcggggtt ccggccgagg
     1681 cgtttcgggt cagcagacaa aggctccgca gcgctatgga cggagttgcc gatagcgtga
     1741 tgttggtgtc cgaacctttt gcggtggcct acggtctgga agcgttgctg cacgcgatga
     1801 ttatcgatat tggcgccggg acagcggatt tctgtgtcat gcaaggccgc tatcccaccg
     1861 aggcggatca gcgcacgttg cctaacgccg gcgactccat cgacgaacgc cttttgaagc
     1921 tgatgcagga ccaccatccc gaggcccagg tttctattca catgattcgt aactggaaag
     1981 aaaaatggag ttttgtcggc gaaccgaaaa gtcgggtgac ggttactgtg ccggccaaag
     2041 gaaaagcaac cgaattcgac atcacagacg aaatgcgcgc ggcctgcgaa tctattctgg
     2101 cgcccatctc ggagaccctg atcgatctgc tttcccgggt tgagcccgag tatcaggagc
     2161 aggtccgcaa taatat
//
LOCUS       DASSWZ010000249         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_631812, whole genome shotgun sequence.
ACCESSION   DASSWZ010000249 DASSWZ010000000
VERSION     DASSWZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631812"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFN58_06015"
     CDS             <1..658
                     /locus_tag="VFN58_06015"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8917580.1"
                     /translation="LPEGPFGEWPGYYASAQRAEPFMRVKALYYRNDPIICGAAPFKP
                     TIHGMYRSCLRAATVWNGMEQAGVPDIRGVYLPPPAQRFMIVVAIKQRYPGHAKQAAL
                     VACQCHAGAYLGRYVVVVDEDIDITDLNEVVWAIATRSDPATSVDILRRTWSGPLDPI
                     IPPGQKGHNSRMIIEATRPYEWRDRFPGVSQISNDTRKQFSAKWEKQLSAVQARHTRP
                     "
     gene            940..1161
                     /locus_tag="VFN58_06020"
     CDS             940..1161
                     /locus_tag="VFN58_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8917581.1"
                     /translation="MEEIMTPSEVAALLKIHLKTVYKLAERGVIPGNRIGRSWRFSRN
                     DVLELVSSKAMSLSDNGDGDSKAAAVAGK"
     gene            complement(1312..1386)
                     /locus_tag="VFN58_06025"
     tRNA            complement(1312..1386)
                     /locus_tag="VFN58_06025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1351..1353),aa:Arg,seq:ccg)
     gene            complement(1411..2223)
                     /locus_tag="VFN58_06030"
     CDS             complement(1411..2223)
                     /locus_tag="VFN58_06030"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET8917582.1"
                     /translation="MTFANFLSVAWEAAMVAGTLIREHWQKPKSLDYKGAIDLVTSVD
                     RQCERRIVEVLQSYFPNHSILAEEETDWVGTEGSHRWIIDPLDGTTNFAHSYPQFCVS
                     IALERDGEVILGLVYDPLRGECFKAVRGGGATLNGVPVRISGVKELDKALLATGFPYD
                     QREEADFYLSFFKAFMTRSQGIRRNGSAALDLCYVACGRIDGFWEMKLRPWDTAAGAL
                     IVEEAGGRLSDLAGNKFSIWGKETLASNGAIHDELVNTAMAATLARNSLTRS"
     gene            2272..>2682
                     /locus_tag="VFN58_06035"
     CDS             2272..>2682
                     /locus_tag="VFN58_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET8917583.1"
                     /translation="MKIRAPAKINLRLRVVGKRADGYHLLDTIMLPVSLYDEIIINKG
                     RKIVKKTALKDRLTVTCDDPLVPQGRQNLVYKAASLVLNEKGINESVRIRIHKRIPVG
                     AGLGGGSTDAAATLVGLNRLFRLGYTLKRLEKMSS"
BASE COUNT          643 a          716 c          727 g          596 t
ORIGIN      
        1 gttgcccgaa ggcccgtttg gcgagtggcc tggctattac gccagcgccc agcgcgccga
       61 gccgttcatg cgagtgaagg ctctgtacta tcgcaacgat ccgattattt gtggcgccgc
      121 gcctttcaag ccgactattc acggtatgta tcggtcctgt ttgcgcgcag ccacagtgtg
      181 gaacggaatg gagcaagccg gggttccaga cattcgtgga gtttatctcc ctccgccggc
      241 gcaacgattc atgattgtcg ttgcgatcaa acagcgctac ccaggccacg cgaaacaggc
      301 tgctctggtc gcctgccagt gccatgccgg cgcctatctg ggtcgttacg ttgtggtggt
      361 ggacgaggac atcgacatta cggatctgaa tgaagtggtc tgggccatag ccactcgctc
      421 cgatcctgcc acctcggttg atattctccg ccgcacctgg agcgggcccc tcgaccccat
      481 tattcctccc ggccagaagg gccataactc acgaatgatc atcgaggcga cccggccata
      541 cgaatggcgt gatcggtttc ccggtgtcag ccagataagc aacgatacgc gaaaacaatt
      601 ctccgctaag tgggagaagc aactgtccgc cgtgcaagcg cggcacaccc gaccgtagat
      661 aaggttagtt tgcgagtcgg agcggactca atccaagcca aatagcaaaa cgggaactgg
      721 ccgaaaaccg taatgattgg ccaattttgt ccatgtcagg cggttcgctg cgctttattg
      781 tgcttaccta gagttctcta cccagtaagg aattctttgg aaatatggtg tcttaggcac
      841 taaggggcgc ccttggagcc aattggcacg gcttgtgctg ccctctgggc tagacaggaa
      901 ataaacagta tttcaagtgt tggaaggaga acaaaacgga tggaagaaat aatgaccccg
      961 tccgaggtgg cagccctgct caagatccat ctcaagaccg tttataagct ggccgagagg
     1021 ggagtgattc caggcaatcg gatcggacga agctggagat tcagtaggaa tgatgtcctg
     1081 gagctagtgt ccagcaaggc gatgagtcta tcagataacg gcgatggtga ctcgaaagcg
     1141 gctgctgtgg ccggaaagta atcgatcatt cctcagagcc gtactatgct atcccccagg
     1201 tcatatcgag cactgtacgg ctggcttcat gagcgaagaa ggtgacttag cccctcaagc
     1261 tcccttgatt ggtagcacta tttcctcatc aaattgtatc agcgctttag ctggtgcgcc
     1321 cgccgagaat cgaactcgga cttagggttc cggagaccct cgtgatatcc ttttcactac
     1381 gggcgcggtg aaatttctac tatcaccggg ttaggatcga gtcaacgagt tgcgtgcgag
     1441 tgtggcggcc atcgcggtgt tgaccagctc atcgtgaatg gcgccattgg aagcgagagt
     1501 ttccttaccc cagatagaaa acttattccc ggcaaggtcg cttaacctgc cgccggcctc
     1561 ctcaacgatc agggcgccgg ccgcggtatc ccacgggcga agtttcatct cccagaatcc
     1621 gtcgattcga ccgcaggcca catagcagag atccagggcc gccgagccgt tgcgtcggat
     1681 gccctgggac cgagtcatga acgccttgaa aaaactcaga taaaaatctg cttcttcacg
     1741 ctgatcgtag gggaagccag tggcgagaag cgccttgtcc aactccttga cgccagaaat
     1801 ccgcactggg acgccgttta gagtggcccc tccacctctg accgctttga aacactcgcc
     1861 ccgtaggggg tcgtaaacta aacctagaat aacttcaccg tcgcgttcca gagcaatgga
     1921 aacgcaaaac tgcgggtaac tatgagcaaa gttcgttgtt ccatcaagcg gatcgatgat
     1981 ccagcggtga ctgccctcgg ttccgaccca atcggtctct tcctcggcta gaatggaatg
     2041 gtttggaaaa tagctctgta acacttcaac aatccgccgc tcacattgcc gatccactga
     2101 agtgaccaag tcgatggctc ccttgtaatc aaggcttttt ggcttttgcc aatgctcgcg
     2161 aatcaaagtg ccggcgacca tggccgcctc ccatgcgact gacaaaaaat tcgcaaatgt
     2221 cattgaagtg gtccttcgca attgaaatta agctatgtta cccaatgaag cgtgaagatt
     2281 cgagcaccgg cgaaaatcaa tttgcgacta cgcgtcgtgg gtaagcgggc ggacggatac
     2341 catctgctcg acaccattat gcttccggta tcgctttacg acgagatcat tatcaataaa
     2401 gggagaaaaa tcgtaaagaa gaccgcactg aaggatcgcc taactgtgac gtgtgacgat
     2461 cccctggttc ctcagggcag acaaaattta gtctacaaag cagcgtccct cgtattgaac
     2521 gagaagggca ttaatgagtc tgtgcggatt cgtattcaca agcgaattcc tgttggggca
     2581 gggttgggtg gtggcagcac ggatgctgca gcaacactag tcgggctaaa ccgacttttt
     2641 cgcctcggat atacattgaa gcggctggaa aaaatgtcat cc
//
LOCUS       DASSWZ010000250         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1310815, whole genome shotgun sequence.
ACCESSION   DASSWZ010000250 DASSWZ010000000
VERSION     DASSWZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310815"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..237
                     /locus_tag="VFN58_06040"
     CDS             46..237
                     /locus_tag="VFN58_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET8917584.1"
                     /translation="MAEGTAWAAVRECRSCGHVGCCDSSTGKHATKQFHETQHPVMRA
                     VPPAAWTWCYVHETQGILS"
     gene            311..1630
                     /locus_tag="VFN58_06045"
     CDS             311..1630
                     /locus_tag="VFN58_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917585.1"
                     /translation="MTSQRVIRSYFLIAGLYTLAAALIWGVNTLFLLAAGLDIFGVFI
                     ANAAFTAGMVVFEIPTGVFADTRGRRASFLMSIAVLCATTLAYVGVAKANGGLLAFAG
                     VSVAMGLGFTFYSGAVEAWLVDALKSTGHDGQLDTVFARGSMVTGVAMLIGTMGGGLL
                     GRIDLALPYLVRSGILVGVFLVAFFTMHDLGFRRRTLRLVDIPAEMKSVAEASIAYGW
                     RRRSVRLLMGVSFLQLGFLTWGFYAWQPYFLRLLGHDAVWVAGVVAAAMALSTIVGNA
                     VVDWFSQFCGRRTTLLLWAAGVQTVAAIGVGLAGSFWLAVIFFLFVAVAVGVTGPVKQ
                     AYIHEMVPSGQRASVISFDSMIGNTGGVLGQSGLGYLSRARSIEEGYVAGGLATVLVF
                     PLLILLRSLGERTDIIVGKRAGRRATCAAQGLPQVAQVDAIAREAAG"
     gene            1800..2582
                     /locus_tag="VFN58_06050"
     CDS             1800..2582
                     /locus_tag="VFN58_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HET8917586.1"
                     /translation="MIDICTTITSLPSALFWGSISVSGIIVGALIGIFAPLTHRAIAA
                     TMAVAAGLLLAAATVELAAKAITLTSSLAGGFAALMIGAVGFSWANSVLSSVGAGDRK
                     RCGLCVSQPSETSNPGSGNAIALGTAMDAIPEALVLGLTLKTHGPEAALIAAIALGNL
                     PEAISGSAGMQAAGRSVKWILGVWGTVAVGTVVLTGAGFLVAGSLSPSLSAALQLFGA
                     GALIAMVTETIIPEAVHGTPKFAGTITAVGFAALLLFAVLAG"
     gene            complement(2637..>2996)
                     /locus_tag="VFN58_06055"
     CDS             complement(2637..>2996)
                     /locus_tag="VFN58_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917587.1"
                     /translation="GGEGRLVRVGKLVPESLASSTVLDREGRQILLESFWAEGPAVLV
                     FMRYFGCLCLSAQITELSPRLFELHQLGIRTVFIGNGAADFIDGFIERFGLADKKIEI
                     VTDPPLEAFFVNGDLCS"
BASE COUNT          552 a          808 c          906 g          730 t
ORIGIN      
        1 gaggattttc cgccgcagag aactccgggc gcatgtgagg agtgcctggc tgagggaact
       61 gcttgggctg cggttcgcga gtgccgatcg tgcggccacg tcggctgttg cgattcctcg
      121 acgggaaagc atgcgaccaa acagttccat gagacacaac atcctgtcat gcgagctgtc
      181 cctcctgcgg cttggacatg gtgctacgtc catgagacgc agggtatcct cagttaatct
      241 caaattgccg cacgacgaag tggtgaatgg tcaaggtttc atcccgggac ccgctccctg
      301 attgcaaggc atgacttcac agcgcgtcat ccgcagctac tttcttatcg ccgggctcta
      361 cacgctcgca gcggctctga tctggggggt taatactctt tttctcctag ccgctggatt
      421 ggacatcttc ggtgtgttca tcgccaatgc agcgtttacc gcgggcatgg tcgtctttga
      481 gataccaacg ggggtattcg ccgatacccg cggccgacgc gcctcattcc tgatgagtat
      541 cgccgtcctc tgcgctacca cattggccta cgtgggcgtc gcaaaagcta atggcggact
      601 gttagcattt gccggggtat ccgtcgccat ggggctcggg tttactttct actccggcgc
      661 cgtcgaagcg tggctagtcg atgcactcaa aagcaccggt cacgacggtc agctcgacac
      721 ggtcttcgcc cgggggtcaa tggtcacagg agtcgcaatg ctcatcggaa caatgggcgg
      781 aggcctgctc ggccgcatcg acctggcgtt accgtacctt gtacggagcg ggatactggt
      841 cggcgtattt ttggtcgctt tcttcacaat gcacgacctg ggattccgtc ggcgtacgct
      901 gcgtttagtg gatattcccg cagagatgaa gagtgtcgca gaggcgagca tcgcgtacgg
      961 ctggcggcgc cgttccgtgc gcctgctgat gggtgtctcg tttttacagt tgggttttct
     1021 aacctggggt ttctatgcct ggcagcctta ctttctcagg ctcttgggcc acgatgcggt
     1081 gtgggtcgcc ggtgtggtgg cggcggcgat ggctctctcc acgatcgtag gaaatgccgt
     1141 tgtcgactgg ttcagccagt tctgcggccg gcgcacgact ctactgttgt gggccgccgg
     1201 cgtgcagacc gttgctgcaa tcggggttgg acttgcaggc tctttctggc ttgctgttat
     1261 ctttttcctt ttcgttgctg tggcggtggg ggtcactgga ccggtcaagc aggcttacat
     1321 ccacgagatg gtgccctccg ggcaaagggc gtcggtaatc tctttcgatt cgatgattgg
     1381 gaataccggc ggagtcttgg gccagtccgg cctgggctat ctgtcccgag cccgatccat
     1441 cgaagaagga tatgtggcgg ggggacttgc cacagtgctt gtctttccct tactcattct
     1501 tctgcgttcc ctcggcgagc gaactgacat catcgttggg aagcgtgcgg gacgtcgggc
     1561 tacctgcgct gctcagggcc tgccgcaggt cgcgcaggtg gacgcaatcg cgcgggaggc
     1621 agcgggatga atatacggat ctgaaaatca tttaacccat gcgcgtgtca gagcggccgg
     1681 acaggtgcaa ccgatttcgt cggtggctaa gagtactgcg gcccggtcaa cgtgcaacgg
     1741 tgcgtgccgt ataacgatag gtagaccctt gggcaattac attctgagga aaggtaaaca
     1801 tgattgacat ctgcacaact atcacgtctc ttccttccgc cttgttttgg ggatcgattt
     1861 ccgtttcggg aataattgtt ggcgcgttga ttggaatctt cgctccgtta acccatcgcg
     1921 ccattgcggc taccatggca gtggccgctg ggttattgct ggccgcggct accgtggaat
     1981 tagccgctaa agccatcacg ttgacatcct ctctggctgg cggttttgct gccctcatga
     2041 ttggcgccgt tgggtttagt tgggcgaatt cggtcctgag ttctgtcggg gcaggggatc
     2101 gaaaacggtg tggcttatgt gtttcccaac catccgaaac ctcaaaccct ggtagcggta
     2161 atgccattgc gttggggact gctatggacg ccatacctga agcattggtg ctcggcctga
     2221 cgctgaagac gcatggtccg gaggcggcgc tcatcgctgc aattgcgctg ggtaatctcc
     2281 ctgaggcgat ttcgggttcc gccggaatgc aggccgctgg acgttctgtc aaatggatat
     2341 tgggtgtttg gggtactgtt gctgtcggca cagtagtctt gacaggtgcg ggctttttgg
     2401 tggccggcag cttatccccg agcctgagcg ctgcattgca gttattcggg gctggcgcac
     2461 taattgccat ggtaactgag acgatcattc cagaagctgt gcatggcacg ccaaaatttg
     2521 ctggaaccat tacagccgtt gggtttgctg ccctattgtt gttcgctgtc ttagcggggt
     2581 aattatttac acccccagtg gcaatcaacg cccaacacct atgaggttcc cggttgtcaa
     2641 gagcacaaat ctccgttaac gaaaaaagct tcaaggggtg gatcggtgac gatttctatt
     2701 ttcttgtcgg cgaggccaaa gcgctcaatg aagccatcga taaagtcggc cgcaccgtta
     2761 ccgatgaata ccgtgcgaat ccctagttgg tggagctcga agaggcgtgg cgaaagctcc
     2821 gtaatctggg cactgaggca aaggcagccg aagtagcgca taaagacaag taccgcaggt
     2881 ccctcggccc agaatgattc cagcaagatc tggcgcccct cacgatcaag aacagtgctc
     2941 gacgcgaggc tttcaggaac gagctttcct acccgcacta gcctaccctc acctcc
//
LOCUS       DASSWZ010000251         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1098734, whole genome shotgun sequence.
ACCESSION   DASSWZ010000251 DASSWZ010000000
VERSION     DASSWZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098734"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..406
                     /locus_tag="VFN58_06060"
     CDS             188..406
                     /locus_tag="VFN58_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917588.1"
                     /translation="MANLYYNDRLIIAHASINQSTKLWSAGAEITWKRDGERQSHTLG
                     GLADRFKTAEEAERFAINLAKTWIDANP"
     gene            complement(455..931)
                     /locus_tag="VFN58_06065"
     CDS             complement(455..931)
                     /locus_tag="VFN58_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917589.1"
                     /translation="MRFLKPIVAILAVLLAILILNSLVETYRWDQRIVEEMNKQGFQL
                     ISQTKGSAPIVPWTLFYPYVTHITLVHPDSVKPFKNFIAADVISFNKDDRGRVESWPR
                     IVVFDCASKLVANLADQQGRYETQIFRPDGVQVKKWWFEMNDQMNSYFCKNIKVTR"
     gene            complement(1001..1564)
                     /locus_tag="VFN58_06070"
     CDS             complement(1001..1564)
                     /locus_tag="VFN58_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HET8917590.1"
                     /translation="MTSIYSRILFLISVICLAVPAAGAAESQGTFEVRIKDHREAIGD
                     FSQLTLTVDEILISPKPGLMSWQSGWKGLAAAPESIDLTKYVGKNSARIFRGSLNTGA
                     FDAIHLKLKEIVGLLKKGQRSAKIKNTIGPIKLPFEIRAQSETMMVLDLVVLDMSDHP
                     PRGYELGVRGYALYTNGKLIDKVPPGP"
     gene            complement(1564..2172)
                     /locus_tag="VFN58_06075"
     CDS             complement(1564..2172)
                     /locus_tag="VFN58_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET8917591.1"
                     /translation="MSISRLLASTTILLSLCVVPAFPHLPIAPKKPEIGRKATQTAVP
                     EFTLVDQNGKSFRFANARGKIVLATFIFTTCPDVCPLLTAKFATIQRTLREKNMDDYL
                     LLSITTDPSRDTPAVLKSYGEQFKADFNHWLFLTGSEPELAKVWKGFGVTVRKTADGQ
                     MQHTALTTLIDRRGVRRVDYYTDKWEEKEILRDMASLDSSNR"
     gene            complement(2162..3058)
                     /locus_tag="VFN58_06080"
     CDS             complement(2162..3058)
                     /locus_tag="VFN58_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET8917592.1"
                     /translation="MRLHLKPFILFVAATLLLVLPASYVLTEGLHKADDEQTVRILTK
                     YLRAVYSRDFKHAYRFVSAEDKRLKEEGVYVRERGPFSGFALDVAKKLSEFIEFAPAQ
                     IKIDDNRARITLAFKAPDMNSLATLLLDWDEERLNALPSHEQKKLLASIDTLKRRSSL
                     EVIEGQEEFTLVREGSNWRLFFDWASGVRVNFMTLVPQDGVIEAQPTIKDTVTQPNEL
                     FTIAYRVKNLTDKEIFARITHRVEPAALTEHLDVVECALLLPVRIPPGEEQSYSSTYV
                     VRGDIPDGTKRIDVTYEFKVEH"
     gene            complement(3152..3733)
                     /locus_tag="VFN58_06085"
     CDS             complement(3152..3733)
                     /locus_tag="VFN58_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET8917593.1"
                     /translation="MKTFNRFSLLYLFLAITAPAASTPVALFLAPRAAQAQDLESKKI
                     VPFVPTPQDVVDKMIELAGVKKGDVVYDLGSGDGRIVITAAKKGARAVGFDVDGDLVK
                     ESRENIRKAGVQELAEIRQQDILTVDLSAASVVTMYLLPDVNLKLRPNVLSQMKPGSR
                     VVSHAFDMGDWKPDKTERVDGRTIYLWTVPVKK"
BASE COUNT          910 a          992 c         1048 g          939 t
ORIGIN      
        1 gccgtcggcg tctatttcaa agacccggac gacaaccagg tagaagtata ttggcagacg
       61 ggtgtggatt ggccccaacc tttcggcaag ccggtggact tgacggcatc ggacgaggac
      121 atcatggcaa acctcacttg agagactgag gagacagacc gctgcttgcc cgagctcgaa
      181 ctattctatg gctaacttgt attacaatga tcgacttatt atcgcgcatg cgtcgataaa
      241 ccagagcacc aagctctgga gcgcgggagc ggaaattact tggaaacgcg atggcgaacg
      301 acagtcacat actctcggcg gtctggcgga tcgattcaag actgctgagg aggcagagcg
      361 ctttgcaatc aatctcgcca aaacatggat tgacgccaac ccgtaactca gagacggcta
      421 agacttaggg cgagagtgca aaagatccag aagtttatct tgtgactttg atatttttgc
      481 agaagtagct gttcatctgg tcgttcattt caaaccacca ttttttcacc tgcacgccgt
      541 ccggcctgaa aatctgggtt tcgtatcgcc cctgctgatc ggcaagattc gcaaccagct
      601 ttgaggcaca atcgaagacc acgattctag gccaagactc gactctgccc cggtcatcct
      661 tgttaaacga tatgacgtct gcggcgatga aattcttgaa cggcttgacg gaatccggat
      721 gaaccagggt gatgtgggta acgtagggat agaataacgt ccaaggaaca atcggggcgc
      781 tgcccttggt ttgagagatc agctgaaagc cttgcttgtt catctcctcg acaatgcgtt
      841 ggtcccaacg ataggtctcg accagactat tcagaatcag gatcgcaaga agaactgcca
      901 gaatggctac gatgggtttg agaaacctca tgacccgctt acctgtggcg attttggcac
      961 aaagagcaaa aaggggccag ctgctttcaa gcggcgccgc tcacggccct ggcgggactt
     1021 tgtcgatgag tttgccgttt gtataaagcg cgtaacctct gaccccgagt tcgtagccgc
     1081 ggggtggatg gtcagacata tccaacacga ctaaatccaa aaccatcatc gtctcgcttt
     1141 gggcgcgaat ctcgaaagga agtttgatgg ggccgatggt gttctttatt ttggcactgc
     1201 gctggccttt cttgaggagg ccgacgattt ccttgagctt taggtgaatg gcgtcgaagg
     1261 ctcccgtatt gagactgccg cgaaaaattc gagcgctgtt tttgccgaca tactttgtca
     1321 ggtcgatgga ttcaggagcc gcagccaaac ctttccagcc gctttgccag gacatgagtc
     1381 cgggctttgg gctgatcaag atttcatcga cagtgagggt cagttgcgaa aagtcgccga
     1441 tggcctcgcg gtgatcttta attcgaactt cgaaggttcc ttgactttca gccgctccag
     1501 cggccggcac ggctagacaa ataaccgaaa tcaagaacag aatccggctg tagatgctgg
     1561 tcattaccgg ttggaggaat cgagggaagc catatccctg agaatttctt tttcctccca
     1621 tttgtcggtg tagtaatcaa ctctacgaac gccgcgccgg tcgatcagag tcgtcagcgc
     1681 ggtatgctgc atttggccgt ccgccgtctt ccgtaccgtc acgccgaacc ctttccaaac
     1741 tttggcgagc tccggctcgg acccggtgag gaaaagccaa tgattgaaat cagccttgaa
     1801 ctgctcccca taacttttca aaacagcagg cgtgtcccgc gatggatcgg tggtgatgct
     1861 cagtaataga tagtcgtcca tgttcttttc ccgcaaggtg cgttggatgg tcgcgaactt
     1921 cgctgtcaaa agcgggcaaa catccgggca ggttgtgaag atgaaagtag ccagcacgat
     1981 cttgccccgg gcattcgcga agcggaaaga cttgccgttt tgatcgacga gggtaaactc
     2041 cggcaccgcg gtttgggttg ccttgcgtcc gatttcaggc ttcttcggcg caatgggcaa
     2101 atgaggaaac gcaggaacca cgcaaaggct caataaaatc gtagtcgatg cgagcagccg
     2161 gctaatgctc aactttgaat tcgtaagtaa catcgatacg cttggttccg tccggtatat
     2221 cgcctcgcac cacgtaggtg ctggaatagc tctgctcctc cccaggtgga attcttaccg
     2281 gcaggagcag ggcacattcg acgacgtcca gatgctcggt gagagctgcg ggttcgacgc
     2341 gatgggtaat ccgcgcaaaa atttccttgt cggtcagatt tttaacgcga taagcgatgg
     2401 taaaaagttc gttcggttgc gtgactgtat ccttgatcgt aggttgagcc tcgatgacgc
     2461 cgtcttgtgg cacgagggtc atgaaattga cccttactcc agaggcccag tcgaagaaga
     2521 gacgccagtt gctcccctct cttaccaagg tgaactcttc ttgcccttca attacttcca
     2581 aactgctcct tcgcttcaag gtgtcgatgg aagcaagcag cttcttttgt tcatgcgatg
     2641 gaagtgcatt gagccgctcc tcatcccagt ccaataataa ggtcgccagg ctgttcatat
     2701 ccggcgcctt gaaggccagt gtgatccgcg cgcggttatc gtcgattttt atttgcgccg
     2761 gagcaaattc gataaactca gacagctttt tggccacgtc cagagcaaag ccggaaaatg
     2821 ggcctcgctc tcgaacgtaa accccctctt cttttaaccg cttatcctct gcggatacga
     2881 atcggtaggc gtgcttgaag tcccgggagt aaacggccct caggtacttg gtgagaatgc
     2941 gcacggtttg ctcgtcgtct gccttatgca gtccttccgt gagcacatag gacgccggca
     3001 aaacgagcaa taatgttgcc gcgacgaaca gaatgaaagg cttaaggtga agacgcatcc
     3061 gccaatttgt ttgagacgct gcctaaggtg cattattcac ggaagaaaaa cccctgcggt
     3121 cgcgggccat gggcaagcaa catgcctcac gtcacttctt gaccgggacg gtccagaggt
     3181 agatcgtacg gccgtctact cgttccgtct tgtcaggctt ccagtcgccc atatcaaagg
     3241 catgggagac gacacgggat ccgggcttca tttgacttag gacgttgggc ctgagtttca
     3301 agtttacatc cggtagaagg tacatggtca ccaccgaagc tgccgagaga tcgacggtca
     3361 agatatcttg ttgtctgatc tcagctagtt cctgcacgcc ggctttgcga atgttttcgc
     3421 gcgattcttt cacgaggtcg ccgtcaacat cgaatcccac ggcccgtgcg cccttctttg
     3481 ccgcggtaat gacgattctc ccgtcaccgg agcccaggtc gtaaaccacg tcgccttttt
     3541 tgacgccggc cagctcgatc atcttgtcca cgacgtcttg tggggtcgga acgaacggaa
     3601 cgattttttt ggattcaaga tcttgggctt gggccgcgcg cggtgcaaga aaaagtgcga
     3661 ctggagtaga agctgcaggg gcggttattg ctaggaaaag atagagcaat gagaagcggt
     3721 taaatgtttt catttgtttt gttgcctcct caataatgga tgaagttttg agtgtgagcc
     3781 gtgaccagat acctctttta attaatcata aaaaaagcac cctcgccagc aggttgttga
     3841 aaaaaactgc ttgcaggctg cccaatagat ttcagagacg gcgcacttt
//
LOCUS       DASSWZ010000252         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_476485, whole genome shotgun sequence.
ACCESSION   DASSWZ010000252 DASSWZ010000000
VERSION     DASSWZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476485"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFN58_06090"
     CDS             <1..333
                     /locus_tag="VFN58_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET8917594.1"
                     /translation="ARTLRVILDLLTVKFLISYSTRPLHVFGVLGLSSGGLGFVIAVY
                     LTLQKLVYHESIGGRPLLLLAILLIFIGFQFLTMGLLAEMLSRTYHESQDKAVFVVGE
                     LLGERKCT"
     gene            330..1634
                     /locus_tag="VFN58_06095"
     CDS             330..1634
                     /locus_tag="VFN58_06095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET8917595.1"
                     /translation="MNLAVIGTGYVGLVAGTCFAESGNDVTCVDIDEQKISLLNTGKV
                     PIYEPGLEEMVRRNAAEQRLSFTTDFDGAVKKASVIFIAVGTPQGPNGDANLDHVRAT
                     AKGIAKAMDGFRIIVAKSTVPVGTADRIRQWVAEETSHPFAIISNPEFLKEGAAVEDF
                     MKPDRVVLGGDNPEALEAVKELYEPFVRTGNPILVMDSRSAEMSKYAANAMLATKISF
                     INEISRLCETKGADVGEVRRAIALDRRIGPHFIFPGVGYGGSCFPKDLRAMISMGADE
                     PEMVLLKAVEKVNERQKGILIEKVTRHFGTALAGLTFAIWGLAFKPRTDDMRDAPSIT
                     VIEALLKAGAKVRAFDPEAIEEAKKIFGDRIQYVNRNYEALTGAAALLVLTEWNEFRR
                     PDFVRIKQLLKNPVIFDGRNLYDPADIRKLGFQYYSIGRNDG"
     gene            1627..2574
                     /locus_tag="VFN58_06100"
     CDS             1627..2574
                     /locus_tag="VFN58_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET8917596.1"
                     /translation="MARYLITGGAGFIGSHLCESFLKQGHDVVCMDNYSTGAQENIAS
                     FASNPRFRFLDHNVSRFIDIGGSLNYVLHFASPASPVDYLELPIPTLKVGSLGTHNAL
                     GLARAKKAVFLLASTSEVYGDPLVRPQNEEYWGNVNPVGPRGVYDEAKRFAEAITMAY
                     HRYHDLDTRIVRIFNTYGPRMRMRDGRVVPNFITQALKGEPLTVYGHGEQTRSFQYVE
                     DLVTGIHRLLESQEHLPVNIGNPHEMTVLQFAEKILELTGSKSSIEYRPLPQDDPQVR
                     QPDISKAKRVLNWEPKVSLDEGLANTIEYFRTRLKEQKV"
BASE COUNT          580 a          671 c          766 g          574 t
ORIGIN      
        1 gcgagaacct tgcgggttat cctcgatctt ttgaccgtca agtttttgat cagctactcc
       61 acccggccgt tgcacgtgtt cggtgttctg gggttgagta gcggcggtct gggattcgtg
      121 atcgcggtgt atcttacatt gcaaaagctt gtgtaccatg agtctatcgg cggcagaccc
      181 ctcctgctgc tggcgattct gttgatcttt atcggctttc agttccttac gatgggttta
      241 ttggctgaga tgttgtcgcg gacatatcat gaatcgcagg ataaagcggt tttcgttgtc
      301 ggagagctat tgggtgaaag gaagtgcaca tgaatctggc cgtgatcgga acgggttatg
      361 tgggattggt cgccggaacc tgcttcgctg agagcggcaa cgacgtgact tgcgtcgaca
      421 tcgatgagca gaagatctcg ctgttgaaca caggcaaggt ccccatctac gagcccgggc
      481 tggaagagat ggtccggcgc aacgcggcgg aacagcgatt gtcgttcacc acggatttcg
      541 atggggcggt gaagaaagcg agtgttattt ttatagccgt gggcactccc caaggcccga
      601 atggtgatgc caatctcgat catgttcggg cgacagccaa aggcatcgcc aaagcaatgg
      661 acggctttag aattattgtc gccaaaagca ccgtgccggt gggaacggcg gaccggattc
      721 gccagtgggt agcggaggaa acatcgcatc cctttgcgat tatttccaat cccgagtttc
      781 tcaaggaagg ggcggcggtg gaggatttca tgaagccgga ccgggtggtg ttgggaggcg
      841 acaatcctga ggctctcgaa gccgtcaagg agctttacga acccttcgtc cggaccggca
      901 atcccattct ggtcatggac agccgcagcg cggagatgag caagtatgcg gcgaacgcga
      961 tgttggcgac gaaaatatcc tttatcaacg agatctcgcg gctgtgcgag acgaagggcg
     1021 cggacgtcgg cgaggtccga agggccatcg ctctcgatcg gcgcatcggc cctcatttta
     1081 tctttcccgg cgttggctac ggcggctcct gttttcctaa agacctccgc gctatgatca
     1141 gcatgggtgc cgatgagccg gagatggtgc tgctcaaagc tgtggagaaa gtgaacgaac
     1201 gacaaaaggg aattttgatc gagaaagtca cccgtcattt cggcaccgct ctcgccggtt
     1261 tgacgtttgc gatttgggga ctggccttca agccgcgcac cgacgacatg agagatgcgc
     1321 cgtcgatcac ggtcatcgaa gcgctgttaa aagccggagc caaagtccga gcctttgatc
     1381 ccgaggccat cgaggaggcg aagaaaattt ttggcgatcg aatccaatac gtcaatcgga
     1441 attatgaggc actgaccggc gccgcggcat tgttggtcct gaccgaatgg aatgaattcc
     1501 gtcgacccga ctttgtccgg atcaagcagt tgctcaagaa ccccgttatc tttgatggcc
     1561 gtaacctcta cgacccggcg gatatccgaa agctcgggtt tcagtattac tcgattggaa
     1621 gaaacgatgg ctaggtattt gatcaccgga ggcgccgggt ttatcggctc gcatctctgc
     1681 gaaagttttc tcaagcaagg gcacgacgtc gtctgcatgg ataactactc caccggggct
     1741 caagaaaaca ttgctagctt cgcttcgaac ccacggtttc gattcctcga tcacaacgtg
     1801 agccggttta ttgatattgg cgggtccctg aattatgtcc tccattttgc ttcgccggcg
     1861 agcccggtag attatctcga acttcccata ccgaccctaa aagtgggctc gctcggcacg
     1921 cacaacgctc taggcttggc gagagcgaag aaggccgtgt ttctcctggc ctcgacgtcc
     1981 gaggtttacg gagatccttt agtgcgaccg caaaatgaag agtattgggg aaacgtcaac
     2041 ccggtcggcc cgcggggggt ttacgacgaa gccaaacgat tcgccgaggc gattacgatg
     2101 gcctaccatc gctatcatga tttggacacg cgcattgtcc ggattttcaa tacctacggg
     2161 ccgcgcatgc gcatgcggga cggacgggtc gtgccgaatt tcatcacgca agcgctcaag
     2221 ggcgagccgc tcaccgtcta cgggcacggt gagcagacgc gaagctttca atacgtcgag
     2281 gatctggtaa cgggcattca tcggctgctg gaatcgcaag aacatctgcc ggtcaatatc
     2341 ggcaacccgc acgaaatgac cgtgctccag tttgccgaga aaatccttga gctcacgggt
     2401 tccaagagct ccatcgaata ccgaccgctc ccgcaggatg atccccaggt acgccagccc
     2461 gacatctcca aggccaagag agtattgaac tgggaaccca aggtgtccct ggacgaaggg
     2521 ctggccaata ccatagagta ttttcggacg cgactgaagg agcagaaagt atgaaaattg
     2581 tggtgacggg c
//
LOCUS       DASSWZ010000253         5806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_985839, whole genome shotgun sequence.
ACCESSION   DASSWZ010000253 DASSWZ010000000
VERSION     DASSWZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985839"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFN58_06105"
     CDS             <1..309
                     /locus_tag="VFN58_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8917597.1"
                     /translation="KEFYDRYGGFTIVIARFIPMIRTFAPVVAGVGAMPYRRFLAFNV
                     FGGIFWVMSTTLAGYFLGTLIPNIQEQIHLVIAIVIFLSLLPGIIKFLSEWRKARSNA
                     "
     gene            356..1174
                     /locus_tag="VFN58_06110"
     CDS             356..1174
                     /locus_tag="VFN58_06110"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET8917598.1"
                     /translation="MDSLGIAVVLGIVEGLTEFIPVSSTGHLILVGHLLGFVGEKAAS
                     FEVAIQLGAILSVVFLYWRRFLDLIPTRSAVHSDSTLSGWAGLWRITLATIPALLVGF
                     LARHTIKEQLFTPLAVTWALAVGGVAILFAEKLVSRRRSYSLETITMGQAFGIGLFQV
                     LALWPGTSRAAATIVGGMLLGLERRGAAEFSFLIAVPIMVAATGYELVKMRGLFATQD
                     LVQFGVGFIVSFLVALIAIKAFVSFLSRWSLAPFAWYRIGVAAIFYFATRGLSL"
     gene            complement(1198..1926)
                     /locus_tag="VFN58_06115"
     CDS             complement(1198..1926)
                     /locus_tag="VFN58_06115"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8917599.1"
                     /translation="MISPRRRLAIRGVIFIVLVGLLFFFSRYLPQPFTALMPPGEDFD
                     DFEAIYSSSDKLSKFLQSLGPYSPAVFVLFQILQVVAAPFPGELTGVAGGYVYGQTMG
                     FVLSTLGLTIGSWVAFELASILGRPFVERFVSQEVLNKFDFLTTNAGATICFLFFLIP
                     GFPKDYLSYLLGLSRMKLSTFLIVSIIGRIPGTYLLTIQGAKFRDQEYYTVAIVAAIS
                     AVILLLAYLYRNQIYHWIKHRHDR"
     gene            complement(2193..4889)
                     /locus_tag="VFN58_06120"
     CDS             complement(2193..4889)
                     /locus_tag="VFN58_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET8917600.1"
                     /translation="MRGFTGLLLLVLLFVVLSFRQGIALYVDWLWFQEVGYTQLFTTT
                     LLYKLILAVLSGGLLAALLYFNLKIAAVAPAGFRFSRVDNVIELPPPDLIDPLFRRLL
                     LPAALFLGLLAAPQGAVNWERLPLFLNATPFNLQDPLFGFDIDFYIFRLPMYSAAYQW
                     LIFALALTLLATAAVYFLYRGIQYGPQGLFLSEKAKTHLLILIALILLLKAAGYLMDS
                     YELVYSSRGAAFGASYTDVYAILPALRILTVLALVAAALCVLQISRPGFRYLIGGVGA
                     LVLVHAVGLNLYPTVIQRFHVVPNEVDAEKPFIERNIKFTRLAYGMDKVESKDFPAEE
                     QLTAADLKRNESTIKNIRLWEHRPLLATYAQLQEIRTYYKFVDVDNDRYMIDGTYRQT
                     MLSARELSHQHLPSRIWINEHLTYTHGYGLVFGPVNQVTPEGLPEFFVKDIPPVVTVE
                     SLKITRPEIYYGELANEYVFVKTTARELDYPAGDQNIYTTYEGRGGVPIDSFWRKLLF
                     SAHHATLRIALSQDVTRQSHILLHRQIQERVKKIAPFLTFDRDAYLVIAQGGRLFWII
                     DGYTISDRFPYSEPTRRRGMNYVRNSVKAVVDAYNGTVNFYLSDPEDPIVLSYGKIFP
                     EMIKPMEQMPEDLRAHIRYPQDLFTIQAHMYATYHMQDPQVFYNKEDLLSIPRRTVGG
                     TEQEMEPYYTIMRLPGETKEEFVLLLPFTPNKRDNMRAWLAARSDPPHYGKLIALDFP
                     KAKLVYGPKQIEARIDQDAFISQQLSLWGQKGSQVIRGSLLAIPIETSLLYVQPLYLG
                     AEKGSLPELKRVIVAFGNQIAMEETLEQSLQRIFGGKPSPQPVQQPVVATQQLTTEKE
                     LARQALGHFSRAQELLRQGDWAGYGEELKKVEALLREMQKSR"
     gene            complement(5029..>5806)
                     /locus_tag="VFN58_06125"
     CDS             complement(5029..>5806)
                     /locus_tag="VFN58_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET8917601.1"
                     /translation="FYLNQERKILGTISVVIFLTIWELIGNTLQLINPMFMSAPSLVA
                     KAAVQLFASGEIWNDLYVSGIEFFWGYGLSIIFAIPFGIAIGWYKRFAYICDPFVNAM
                     NATPRVALLPLVIIWLGIGILSKVGIIFLGAVFPLLINTRDGVKTTPANLLTAARSFG
                     ASEWQIFKSVVLPSTVPFILTGLRLAVGRALIGVMVGELYAATAGIGFMITVAGATFQ
                     TDKVFVGVLIFAITGMVLTDVIDRIEHRFDKWRPKVGADA"
BASE COUNT         1408 a         1449 c         1644 g         1305 t
ORIGIN      
        1 aaagaatttt atgatcggta cggcggattt acgatcgtca tagcccgctt cattcccatg
       61 atcagaactt tcgcccccgt cgtggcgggt gttggggcaa tgccctacag gcgcttcctg
      121 gctttcaatg tcttcggtgg aatcttctgg gtgatgtcga ccaccttagc cggctatttt
      181 ctgggcacac tcatcccgaa tatccaggag caaattcatc tggtgatcgc catcgttatt
      241 tttctttcac tgctccccgg catcattaag ttcctcagtg aatggcgcaa ggcaaggtct
      301 aacgcctgaa tggaggttgg cgttattttg acagggtaga ctaggaggag gatgtgtgga
      361 ctctctgggc atcgccgttg tcttaggtat tgtggaagga ctcacggaat tcataccggt
      421 gtcttctacc ggtcacttga tcctggtcgg ccatctgctc ggttttgtcg gcgaaaaggc
      481 tgcaagcttc gaagtcgcca ttcagctcgg cgcgatccta tcggttgtat ttctgtactg
      541 gcgacgattc ctggacttga ttcccacccg atctgccgtt cattcggact cgactttaag
      601 cgggtgggcc ggcctttggc gcatcacact ggcgacgata cccgccttgc tcgttggatt
      661 tctcgcccgc catacgatca aagagcagct ctttactccg ctggcggtca cctgggcgtt
      721 ggccgtggga ggcgtcgcga tcttgttcgc tgagaaactt gtttcccgac gccgctccta
      781 ttcacttgaa accatcacca tgggtcaggc cttcggaatc ggcctcttcc aggtccttgc
      841 cttgtggccg ggcacgtcga gagcagcggc aacgattgtc ggtggaatgc ttctgggact
      901 cgaaagaagg ggagcggcgg agttctcttt cctgattgcg gtgccgatca tggtcgccgc
      961 cacaggctat gagctggtca aaatgcgcgg tctttttgcc acgcaggatc ttgttcagtt
     1021 tggggtgggg tttatagtat catttttagt ggcgcttatc gcaatcaagg cttttgtaag
     1081 tttcctcagt cggtggagcc tcgcaccatt cgcgtggtac cgcatcgggg tggcggcgat
     1141 tttctacttc gcaacgcgcg gcctgagtct gtaaagcgcc cgtttccgtc ttggctttca
     1201 gcgatcatgc cggtgcttga tccagtggta aatctggttt cggtagagat atgccagaag
     1261 caagatcacc gcggagatcg cagcgacgat agccacggtg taatattctt gatcacggaa
     1321 cttggcaccc tgaatggtta gcagataagt tccgggaata cggccgatga tggatacgat
     1381 gaggaaagtg ctgagcttca ttcggcttaa gcccaggaga taagaaagat agtccttcgg
     1441 gaagccggga ataagaaaga atagaaagca aatcgtggcg cctgcattcg tggtgaggaa
     1501 atcaaacttg tttagcacct cctgactgac aaatcgctcg acaaaaggcc tgcctaagat
     1561 acttgccagc tcgaatgcga cccaggagcc gatggtgagt cccaacgtgg aaagcacaaa
     1621 gcccatggtt tggccgtaca cgtatccgcc ggcgaccccg gttagttctc cgggaaaggg
     1681 agcggccacc acttggagta tttgaaaaag cacgaatacg gccggtgagt aaggacctaa
     1741 agattggaga aacttactga gcttgtcgct gctggagtaa atcgcttcga aatcatcgaa
     1801 gtcttcgccg ggcggcatta atgcggtgaa cggttgcggc aggtagcgac tgaaaaaaaa
     1861 cagcagccca actaagacga taaagataac tcccctgatg gcgagccgcc gccgtgggct
     1921 gatcatcgga gttgtatagc agcaaaaaat gcgaaaaacg agacttgata gtccacacgc
     1981 cgagctgtaa aaaaaagtaa actttcctgg tgacggaatc gttggtaaaa cgtaaaagca
     2041 tatttaactg cttacgaggc cgagcgcagt aaaattccct cgcgcttctc caagtcgccg
     2101 aagatctctt ggtgagtggt cgttacacgg cggccaaaag atcgtctggc caaatttatg
     2161 atcacgcaat ctgcgtgctg gaagcggggc ttttaacggc ttttttgcat ttctcgcaac
     2221 aaggcctcga ccttcttgag ctcctcgcca tagccggccc agtctccttg gcgcaggagt
     2281 tcttgggcgc gtgaaaagtg cccgagcgcc tgccgggcta gctccttttc cgtagtaagc
     2341 tgctgggttg caaccacggg ctgctgtacc ggctggggtg agggtttgcc gccgaaaatt
     2401 ctttgcagcg attgctccag ggtctcctcc atggcgattt gattgccgaa agcgacaatc
     2461 actcgcttga gctccggtaa ggagcccttt tccgcgccaa ggtagagggg ttgaacatag
     2521 agtagcgagg tctcgatggg gatggcgagc aggctgccac gaatcacttg cgatcccttt
     2581 tggccccaga gactcagctg ttgcgagatg aaagcgtctt gatcgatgcg tgcttcaatc
     2641 tgtttgggac cgtaaaccaa tttggctttc gggaaatcca gtgcgatgag cttgccatag
     2701 tgcggtggat cgctacgcgc cgccagccaa gcgcgcatgt tgtctcgttt gttcggcgtg
     2761 aaaggtagaa gcagaacaaa ctcttctttg gtctcacccg gcaagcgcat gatggtgtaa
     2821 tagggctcca tttcttgttc cgtgccgccg acagtccggc gcgggatgct gagcaggtct
     2881 tctttgttgt aaaagacctg cggatcttgc atgtgatagg tggcgtacat gtgagcctgg
     2941 atggtaaaca ggtcctgtgg gtaacggatg tgagcgcgca gatcctctgg catctgctcc
     3001 atgggcttga tcatctcggg gaaaattttt ccataggaca aaacgatagg atcctcggga
     3061 tcgctcagat agaagttcac tgttccattg taggcatcga ccacggcctt caccgagttg
     3121 cgaacatagt tcattccccg gcggcgtgtg ggttccgagt aagggaaccg gtccgagatt
     3181 gtgtatccgt caattatcca aaaaagcctt ccgccttggg cgatcaccaa gtaagcatca
     3241 cgatcgaaag taagaaacgg ggcaatcttt ttcactcgct cctgaatctg gcgatgcaat
     3301 aggatgtggc tttgacgggt aacatcttgt gacagagcga tcctcagagt ggcgtgatgc
     3361 gctgagaata aaagcttgcg ccagaacgaa tcgatcggca ccccgccacg accctcgtaa
     3421 gtcgtataga tgttctggtc gccagcgggg tagtccaatt cacgagccgt agttttgacg
     3481 aaaacgtatt cattggcgag ttcaccataa tagatctcag gtcgcgtaat cttcaacgat
     3541 tcgacggtga cgacgggcgg gatgtcctta acgaaaaact ccggtaagcc ctcaggggtg
     3601 acttgattta ccggcccgaa gaccaggccg tagccatgcg tataagtgag atgctcgttg
     3661 atccagattc ggcttggcag gtgctggtgg gagagttcac gcgccgacag catggtctgc
     3721 cggtaggtgc cgtcgatcat gtagcggtcg ttgtcgacgt cgacgaattt gtaataggta
     3781 cgaatctctt gaagttgcgc gtaggtcgca agcagcggcc tatgttccca aagccgaatg
     3841 ttcttaatag tcgattcatt gcgcttcaga tcggcggcgg tcagctgctc ttcagcgggg
     3901 aaatctttgc tttccacctt gtccatccca taggcgaggc gcgtaaattt gatattccgc
     3961 tcgatgaaag gcttttcggc gtcgacttca ttgggtacga cgtgaaaacg ctgaatcact
     4021 gtaggataga gattgagtcc aacagcatgg accagaacta gcgcacccac accgccgatc
     4081 agatatcgaa atcccgggcg tgagatctgt aagacgcata gcgccgcagc gaccaaagcc
     4141 aggacagtca gaatacggag cgcgggtaga atcgcgtata cgtccgtgta actcgcgcca
     4201 aaggcggcac cgcgggatga ataaaccaac tcataggagt ccatcaggta gccggccgct
     4261 ttcaaaagca gaatgagagc gatcaggatc aacaggtggg ttttggcttt ttcggaaaga
     4321 aaaagtcctt gaggaccgta ttgaatacca cggtagagga aatagacggc cgcggttgcg
     4381 agcagcgtca gtgccaatgc gaaaatcaac cactggtaag ccgccgaata catcggtagc
     4441 cggaagatat aaaagtcgat gtcgaaacca aacaaaggat cttgaaggtt aaaaggcgtc
     4501 gcattcaaaa aaagcggcaa acgctcccag tttactgcgc cttggggcgc cgcgagcagg
     4561 cccaagaaga gcgccgccgg aaggagcaaa cgtcgaaaca gcgggtcgat gagatcagga
     4621 gggggaagct cgataacgtt atccacgcgc gagaagcgga agccggcagg ggcgacggcg
     4681 gcaatcttaa gattgaaata cagcagcgca gcaagcaggc cgccggacaa aaccgccaag
     4741 atgagcttat acaaaagagt cgtggtaaaa agctgcgtgt agcccacttc ctgaaaccag
     4801 agccagtcca cgtaaagggc gatcccctgt cggaaagaca ggaccacaaa aagcaagacc
     4861 agtagcagga ggccggtgaa tccgcgcatt agatctcttc tttagctcgt attcttgagc
     4921 ggcacgcaaa agcggattga caatgcggtg accggataaa tgcaaaaaaa aggtggcgcc
     4981 ggaattctcc gaaacgccac ctcggtgatg ttatgttgtg ttcagcgttt atgcgtcggc
     5041 accgaccttg ggacgccact tatcaaatcg atgctcgatg cggtcgatga cgtcggtcaa
     5101 gaccataccg gtgattgcaa agatcaggac gccgacgaag accttatcag tttgaaaggt
     5161 cgcgcccgcg accgtgatca tgaaaccgat acccgcggtt gccgcataga gctcgccgac
     5221 catgacaccg atcagtgccc ggcctaccgc caagcgcaat cccgtcaaga tgaagggcac
     5281 agtggaaggc agcacgacgg acttaaaaat ttgccattct gaagcgccga agcttctagc
     5341 ggcggtaagg aggttggcag gggtcgtctt tacgccgtct cgagtgttta tcaatagagg
     5401 gaaaactgcg cccaggaaga taatcccgac tttggaaaga ataccgatgc ctagccaaat
     5461 gataaccaga ggcaggaggg cgacgcgagg agtcgcgttc atcgcgttga cgaaagggtc
     5521 acaaatatag gcaaatctct tgtaccaacc gatggcgata ccaaacggga tggcgaagat
     5581 aatggatagg ccataacccc agaagaactc gatgccgctg acatacaagt cattccaaat
     5641 ctctcctgag gcgaagagct gaaccgctgc ctttgccact aaggaaggcg cgctcataaa
     5701 catggggttg ataagctgca gcgtgttgcc gattagctcc catatagtca agaaaataac
     5761 cacggatatc gttcccagga tcttcctttc ctggttgaga tagaac
//
LOCUS       DASSWZ010000254         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_603962, whole genome shotgun sequence.
ACCESSION   DASSWZ010000254 DASSWZ010000000
VERSION     DASSWZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603962"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFN58_06130"
     CDS             complement(<1..287)
                     /locus_tag="VFN58_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917602.1"
                     /translation="MRRMIVTIFALLFLGKMTAGARAQERLNLAYISPTAGSSSIFWV
                     AKDMGIFKKHGLDVNVIYIEGTPKALMSLFAGDLQVIAGTGPAVVSAKLRGG"
     gene            complement(329..1051)
                     /locus_tag="VFN58_06135"
     CDS             complement(329..1051)
                     /locus_tag="VFN58_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917603.1"
                     /translation="MVLAAEGVAVGSVVVAAVSGAAAPRGVGKMINAETFFTAEEQQR
                     IRQAVVTAEQRTAGEIVPMLVGASARYAEIEIAGMGVGLVAGTLAAFLIQDPWAAIHS
                     QLLWPLAGAALGSVLCSLPVVKRNLIPKDRILEAVHLRSLAAFTAQGLHYTRAHTGIL
                     ILASLLEHRVEVLADTGINEKVPAGTWDEVVQILTVGLKSGNACDAYCRAIERCGEIL
                     AQHFPRPPDDQDELGNKLVIER"
     gene            complement(966..1892)
                     /locus_tag="VFN58_06140"
     CDS             complement(966..1892)
                     /locus_tag="VFN58_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET8917604.1"
                     /translation="MKLRKARMLFAGLALLLAPTIFSLALALDVPPLRGRINDYAGLI
                     PADRAQALEERLARFEAETGHQIAVLTIPSLKDDSLEDFSIRVADAWKIGKKGFDNGA
                     ILLIARDDRKLRIEVGYGLEGVMPDAIASRIIREVITPRFRSGDYAGGIEAGIDTILK
                     VTKGETLPERTRRAPGPTTSQGSSLITILIITAMLALFIGMTRRRLLSGAVGGAASGL
                     VTSLFASGGLGFVLFPALIVGALLGAIGAALGTGSAGNQWVGRSRSRRGDWGGGAFPG
                     GFGGGGFGGGGGGGGFSGGGGGFGGGGASGSW"
     gene            complement(1900..2496)
                     /locus_tag="VFN58_06145"
     CDS             complement(1900..2496)
                     /locus_tag="VFN58_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET8917605.1"
                     /translation="MRKIARDGNYFLLMALAAFSISGCGYNQLQGLDEQVKAAWAEVQ
                     NQYQRRADLIPNLVSTVKGASQFEQETLQKVIEARAQATSIKLDAQALSDPATFKKFE
                     QAQQQLSGALSRLMVVVERYPDLKANQNFRDLQAQLEGTENRIAVARKRYVESVAEYN
                     KGIRFFPTNLTAKYLLGLKERETFSADEGAAKPPQVKF"
BASE COUNT          622 a          809 c          753 g          631 t
ORIGIN      
        1 ccgccgcgga gctttgcgct gacaacggct ggcccggttc ccgctatcac ctggagatcg
       61 ccggcgaaga gcgacatgag cgccttaggt gtcccctcga tataaatgac gttaacgtcg
      121 aggccgtgtt tcttaaatat acccatgtcc ttggcgaccc agaaaatgga agatgaaccc
      181 gcggtcggag aaatataggc taaattgagt cgttcctgag ctcgtgcacc cgctgtcatt
      241 ttgcccagaa acaatagcgc gaaaatagtt acgatcattc gacgcatagg atgcctcctt
      301 tctcggtttt tccagcagca ggttttcctt atcgctctat cacaagtttg ttaccgagct
      361 cgtcctgatc gtccggtggg cggggaaagt gctgggctag aatctcaccg cagcgttcga
      421 tggccctgca gtaggcatcg cacgcgttac cggattttag gccgacggta aggatctgaa
      481 ccacttcatc ccaagttcca gcgggaactt tctcgttgat gcctgtgtcc gccaagactt
      541 ccacgcgatg ttccagcaaa gaggcgagaa tcaatatgcc ggtatgtgcc ctagtgtaat
      601 gcagtccctg cgccgtgaac gccgccagac ttctcaaatg gactgcttcc aagattcgat
      661 ccttaggaat caaatttctc tttacaactg gaagtgagca gaggacagat cccagtgccg
      721 cgcccgcgag aggccagagt agttgtgaat ggatggctgc ccaagggtct tgaataagga
      781 acgccgctag cgtacccgcg accaacccga cacccatgcc ggcgatctcg atctcagcgt
      841 agcgggcact tgcgccgacg agcatgggta cgatctcgcc ggccgtccgt tgttcggcgg
      901 tgaccacggc ttggcgaatg cgttgctgtt cctctgcggt aaaaaaagtt tccgcattga
      961 tcatcttacc aactccccga ggcgccgccg cccccgaaac cgccgccacc accactgaac
     1021 ccaccgccac cccctccgcc gccaaaacca ccgcccccga aacctccggg aaaggcaccc
     1081 cctccccaat ctcctcgtcg agatcttgat ctacctaccc attgatttcc cgcagatcct
     1141 gtacccagag cagcgcctat ggctcccagc agggctccaa caatgagagc ggggaacaaa
     1201 acaaatccaa gccctccgga agcaaacaat gaagtgacta gaccggaagc cgcccctcca
     1261 accgcgccgc tcaacaatct gcgtcgcgtc atgccaataa aaagagcaag catggcggtg
     1321 attatgagaa tggttattag cgacgagccc tgagaagtgg ttggtccagg agcccggcgg
     1381 gttcgctccg gcagggtttc tcctttggtg actttgagaa tcgtatcgat gccggcctca
     1441 atcccgcccg catagtctcc ggaccgaaag cgcggggtga tcacttcccg gatgatgcgg
     1501 ctagcaatag catccggcat gactccctcg agaccatagc cgacctcaat gcgaagcttt
     1561 cgatcgtcgc gagcgatgag gaggatcgcg ccgttatcga agcctttctt gccgatcttc
     1621 caggcatcgg cgaccctgat gctgaagtct tcgaggctgt catctttcag gctggggatg
     1681 gtcaaaacgg cgatttggtg gcccgtttct gcttcaaagc gggccaggcg ctcttcaagc
     1741 gcctgggctc tatcggcggg gatcaatcct gcgtagtcgt tgattcttcc ccgcagcggc
     1801 ggtacgtcca gcgctagggc aagcgagaaa attgtcggcg cgagcaggag ggcaagcccg
     1861 gcaaaaagca tccgcgcttt gcgcagcttc atctgctgct cagaatttta cctgcggagg
     1921 cttcgccgca ccctcgtcgg cgctgaaggt ctctcgctct ttgagcccaa gcagatattt
     1981 tgccgtcagg ttcgtaggga agaagcggat ccctttgttg tattcagcga cgctctcgac
     2041 gtaacgcttg cgggcgaccg cgatgcggtt ttctgttcct tccaactggg cctggagatc
     2101 gcgaaagttt tgattcgctt taagatcggg ataccgttcg acaaccacca tcagacgaga
     2161 taacgcgccg gacaattgtt gttgtgcttg ttcgaatttc ttgaacgttg ccggatcact
     2221 gagagcttgc gcatccagtt tgatcgacgt cgcttgtgct cttgcctcga tgactttctg
     2281 cagagtctct tgctcgaatt gagacgcgcc ttttactgtt gagacgaggt tagggataag
     2341 gtcagcacgg cgttgatatt ggttctggac ttcagcccaa gcagctttga cttgctcatc
     2401 caaaccttgg agctggttgt agccacagcc ggatattgaa aatgcggcta gagccataag
     2461 tagaaaatag tttccgtctc gcgcgatttt gcgcataatt gtctccccct ctctgttgga
     2521 aagttagatc cagccccctc tatctcaaaa gggtactgac tttgtcaaga aagtaatcgc
     2581 ggtgatgatg ttgggcacag acgcacgcgg gcagaaacta tgaactaaat agggcctggc
     2641 gaagatacca ggccctatag caaggcaggc aggtcagggc actaggatat tgaatcgatt
     2701 attcggctga tcccacttta gggcgccact tgttaaaccg tccttccacc cgagtcaata
     2761 gctccatacc gagcatcccg gtcagtgcga agatgaggac gccgacgaag acctt
//
LOCUS       DASSWZ010000255         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1396091, whole genome shotgun sequence.
ACCESSION   DASSWZ010000255 DASSWZ010000000
VERSION     DASSWZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396091"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFN58_06150"
     CDS             complement(<1..717)
                     /locus_tag="VFN58_06150"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917606.1"
                     /translation="MNKIFVALLILSLCHTSVQAADKIRISTPGDAAHFTMHLAQKRG
                     FLKEEGFDAEVIVISGPVANVALSNGDTDYFSGFGSALRAILQGLPLRIVACYRPTPH
                     FVLQSRPEFKTVKDLKGKTIGITAFGGGPDLVGRLMIKHFGLDPDKDVKFIPGGSSEG
                     RLIRMQQGLLDATMASVPADYLGRKMGFPIIVRSEDLFTYPFSGVTTHIKKIKEKPDE
                     VKRVIRAGIKANRYMRENRDG"
     gene            complement(787..1734)
                     /locus_tag="VFN58_06155"
     CDS             complement(787..1734)
                     /locus_tag="VFN58_06155"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917607.1"
                     /translation="MINLVVILLTTLVLVKSADSADKVRISISNLSGQFMTYPLALKR
                     GFLQEEGIEAEIIRVTGGAATAAMSSGDLDYGTGMSVGGAITGALPVKVVACFVPAPV
                     FALVARPEIKSVQELKDKTVGISTFSGLSIFGARVIAKHYGLDPDKDLKFVAIGAVEG
                     RFIRLTQGLVDAAILAPPLDSEAKKKGFNILARAEDILTFPETGLVAGVKKIQAKPDE
                     VKRVIKAGIKANRYIRGNRDGTIQFIMEWLKVNKEVAATTYDGVVKVYNEDPSVCEKG
                     LRVMIEERKQTLKITRDVPLSEVADLSLIREAQREMGAR"
     gene            complement(1806..2795)
                     /locus_tag="VFN58_06160"
     CDS             complement(1806..2795)
                     /locus_tag="VFN58_06160"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917608.1"
                     /translation="MGRKVFVLICLIVTVLSITGALAEAQQHAKVAKIGFLGAGGLGG
                     VGSGSELFKRELRALGYVEGKNIAIEYRSTEGKRDRLPAVADELVRIKVDVIVTSSTI
                     AALAAKNATKTIPIVFYTVTDPVADGLVDSLARPGGNITGITDIGAVLSGKRLELLKE
                     TIPKLSRVAVLWDPQNPGNAQSWKESQLPARELGLQLHSMEVSSTDKYEGAFKETIKA
                     RSAALAVISSPLVSSNRKQIADLAEKNRLPAIYQREDYVASGGLMSYGPDRAEPYRRA
                     AAILVKILKGTKPADIPVEQPTKFEFVINLKAARQIDLTIPPDLLARATKIIR"
     gene            complement(2848..>3716)
                     /locus_tag="VFN58_06165"
     CDS             complement(2848..>3716)
                     /locus_tag="VFN58_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917609.1"
                     /translation="DSRAAESGRLEFFRQGLRELGYIEGKNLIIEYRYADGRPERLPA
                     LAEELVRLKVDIIVTDPAAPARAARKATSTIPIVMVSAGNPIQAGLVASLARPGGNVT
                     GLTGLTSELLGKRFELLKEVVPKVSRFAFLDDDDILDLSPFQYAQKAAQALAVSLDRV
                     EVKTANPDFDGAFRMMVKERVGGVITSAGFLSTTLHRKKILELLEQARIPAIHGSITW
                     IDDGGLMYYGANSSDLYRRAATFVDKILKGTKPAELPVEQPKKFDFIINLKAAKQIGP
                     PNVLARADKVIR"
BASE COUNT          806 a         1046 c          947 g          917 t
ORIGIN      
        1 cccatcgcga ttctccctca tgtagcggtt ggctttgatc cccgcccgaa tgacccgttt
       61 gacctcgtcg ggtttctctt tgatcttttt gatgtgagtt gtcacgccgc tgaaaggata
      121 ggtaaacagg tcttctgatc gaacaatgat tggaaagccc atttttctgc caagataatc
      181 ggcgggtaca gaggccatcg tggcatcgag caatccctgt tgcattctga tcagccgtcc
      241 ttcactggac cctccgggaa tgaacttcac atccttgtcc ggatcgagtc cgaagtgctt
      301 gatcatcaac cggccgacca gatcggggcc gccaccaaat gcagtgatcc cgatggtctt
      361 gcccttgaga tctttaacgg ttttgaactc aggtcggctc tgcaatacaa aatggggtgt
      421 cggccgatag caggcaacga tccgaagcgg taagccttga agaatcgccc gcaaggccga
      481 gccaaaaccg ctgaagtagt cggtgtcgcc gttgctaaga gcgacgttgg ccaccggtcc
      541 tgaaatgaca atcacttccg cgtcgaagcc ttcctctttt aggaatcctc ttttctgtgc
      601 cagatgcatg gtgaagtggg cagcgtcccc gggtgtgctg attctgatct tgtcggccgc
      661 ttggaccgaa gtgtggcata aggaaaggat cagcaacgcg acaaagatct tgttcattat
      721 ttgtgcctcc cgttcctatc caaatcctaa actctgctct cgttaggctc cgcggccgct
      781 ggatatttac ctggctccca tctcacgctg cgcctctctg atgagcgaga gatccgctac
      841 ttcgctcagc ggtacgtcgc gagtaatttt caaggtctgc tttctctcct caatcatcac
      901 acgcaatcct ttttcacaga cacttggatc ctcgttgtag accttgacta caccgtcata
      961 ggtggtcgcg gcgacctctt tattgacctt cagccactcc ataataaact ggatagtgcc
     1021 gtcgcgattg cctcgaatat agcggttggc cttgatgccg gccttgatga cacgcttgac
     1081 ttcatccggt ttcgcttgga tttttttcac accagccacc aaacccgtct ccggaaaagt
     1141 aagaatatcc tcagcccggg caagaatgtt gaagcccttc tttttcgcct cggaatcgag
     1201 agggggcgcg agtatggccg catcgaccaa gccctgggtt aacctgataa agcggccctc
     1261 aactgcgccg atggcaacaa acttgagatc tttatctgga tcgaggccgt agtgcttggc
     1321 gatgacccgc gcgccaaaga tggatagacc gctgaaagta gaaattccaa ccgttttatc
     1381 cttaagctct tggaccgatt tgatctccgg ccgcgcaacc agcgcaaaaa ccggagcggg
     1441 cacgaaacag gctaccacct tgacgggtaa tgctccggtg atagctcctc ctacagacat
     1501 accggtgcca tagtccaaat ctccgctgct catcgctgcg gtggctgcgc cgccagtgac
     1561 cctgatgatt tcagcttcaa tcccttcttc ttggagaaag cctctcttga gcgccaaagg
     1621 gtaagtcatg aactgcccgc tcaggttaga gatactgatt ctaactttgt ccgccgaatc
     1681 ggctgatttg acgagaacaa gtgttgtgag caaaataacc actaagttaa tcatacactg
     1741 ctccagaatt tcccttccgc ttaccgtccg gagcctgtcc ctagcagcga cgcaggaact
     1801 cacgcctacc gaatgatttt ggtcgcccgc gccaataggt cgggtggaat cgtaaggtca
     1861 atctgcctcg ccgccttcag attgataacg aattcgaatt tcgtcggctg ctcgactggg
     1921 atgtcggcgg gcttggtgcc cttgaggatt ttgaccagaa tagcggcggc gcgtctgtaa
     1981 ggctcggccc tgtcgggtcc gtaggacatg aggccaccgc ttgcgacata atcttcccgt
     2041 tggtagatcg ccggcagccg atttttttcc gctaggtccg cgatctgttt tcgattagaa
     2101 gagaccaatg gactcgatat tactgcaaga gcggcgctac gcgccttaat tgtctcctta
     2161 aacgcgcctt cgtatttgtc ggtgctgctt acctccatag aatgaagctg cagacccagc
     2221 tctcgtgctg gcagttggct ttctttccat gattgcgcat tgcccggatt ctgtggatcc
     2281 cacagcaccg cgacgcggga gagcttggga atggtttcct tgagtaactc tagtcgtttg
     2341 ccagacaaca ccgccccaat gtcagtgatt cctgtaatgt ttcctccagg ccgcgctagg
     2401 ctgtcgacca gcccatccgc aacaggatca gtcacggtgt aaaaaacgat gggaatcgtt
     2461 tttgtagcgt tcttggcagc tagagcagca atggtcgagg acgtgacgat tacgtcaact
     2521 ttgatacgga ccagctcatc ggcaacagcg gggaggcggt cacgcttacc ctccgtggat
     2581 cgatattcga tagcaatgtt cttgccctca acatagccca gtgcacggag ttctcgcttg
     2641 aataactcgc tcccggagcc tacaccacct aaaccaccgg ctccgaggaa cccaatctta
     2701 gccacctttg catgctgctg cgcctcggct aaagcgccag tgatcgaaag gacagtgact
     2761 attagacaga taagaacaaa gacttttctg cccatccgtt tgtttcttgt tcttttggta
     2821 ctaataatca ctacaacctg tttattgtta tctgatcact ttatctgccc gcgccaagac
     2881 atttggcggg ccgatctgct tggcggcttt cagattgatt atgaagtcga atttcttcgg
     2941 ctgctctacg ggcagctccg ccggcttggt gcctttgagg atcttgtcca cgaacgtggc
     3001 cgcgcgccgg tataagtcgg aggagtttgc cccgtagtac atgaggccgc catcgtcgat
     3061 ccacgttatg ctcccgtgta tggctgggat tcgggcctgt tcgagcagct ccaaaatctt
     3121 ttttcgatgc agcgttgtac ttagaaagcc ggcgctagta atgacaccgc cgacgcgctc
     3181 cttgaccatc atccggaatg cgccgtcgaa atctggattg gcggtcttta cctctacacg
     3241 gtcaagacta acggctaaag cctgggcggc tttctgcgcg tattgaaacg gtgacaagtc
     3301 gagaatgtca tcgtcatcga gaaacgcaaa acgcgagacc ttagggacaa cttcttttaa
     3361 taactcgaat cgcttaccaa gcagctccga agttaagcct gtcaaccccg tgacgtttcc
     3421 gccgggccga gcaagactgg ccactaatcc ggcttgaatc ggattgccgg cgctcaccat
     3481 cacaatgggt atcgtcgagg tggccttcct ggcggcgcga gctggcgcag cgggatcggt
     3541 aacgatgatg tcaaccttga gacgaacgag ttcctcagcc agcgccggca aacgctcagg
     3601 tctcccgtcg gcatacctat actcaatgat aaggtttttt ccctcgatgt agcccaactc
     3661 ccgcaaacct tggcggaaga attccaggcg gccggactca gccgcgcggg agtcag
//
LOCUS       DASSWZ010000256         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1396257, whole genome shotgun sequence.
ACCESSION   DASSWZ010000256 DASSWZ010000000
VERSION     DASSWZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396257"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFN58_06170"
     CDS             <1..707
                     /locus_tag="VFN58_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET8917610.1"
                     /translation="IGNSSDRPVALAELIGILVNDGVRRPNTELSNIHFARGTPYETV
                     FERKTEPGQQVLVPEVARAVRKAMAGVVESGTARRLAGVFRLSDGKVITVGGKTGSGD
                     NRIETFNRWGNVTSSRATNRTAAFVFYIGNRYFGVITAYVQGREAEHYHFTSSLPVTL
                     LKLLAPTVIAKLDKNVVQPPPAPGDAIQNDGSTRKINFKISAKSPSEARFRDHKRPIS
                     IDLKLGTTPISEQLDL"
     gene            complement(669..1355)
                     /locus_tag="VFN58_06175"
     CDS             complement(669..1355)
                     /locus_tag="VFN58_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET8917611.1"
                     /translation="MQIAEIFHSIQGEGRFVGMPSVFIRTSGCNLRCVWCDTPYTSWA
                     PEGKKWSVGEILSEVAKYPSRHIVVTGGEPFLAAEIEELTAGIKPLGSHITIETAATL
                     FKPVACDLVSMSPKLANSTPWRRAKGKFAKMHETARLNFEVMQQFIDTYDYQLKFVVD
                     QREDFDEIEEILGRLENVDRPRVLIMGQGKTRRELRDKTKWLIEACKEYGFGYTPRLQ
                     IELFGNRRGT"
     gene            1517..1759
                     /locus_tag="VFN58_06180"
     CDS             1517..1759
                     /locus_tag="VFN58_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917612.1"
                     /translation="MSQDKEALLAEERKLRRLSRAMDIAAILLWEVDLTLEEAQEIVT
                     HAKHTALQLFPDKEETFDLIYGPRFRRILTEKYQLQ"
     gene            1945..>2775
                     /locus_tag="VFN58_06185"
     CDS             1945..>2775
                     /locus_tag="VFN58_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HET8917613.1"
                     /translation="MSQNWIHIRGAKQNNLKNLDLQIPLNALTVITGVSGSGKSTLAF
                     DILYAEGQRRYVESFSAYARQFLDRMDKPDVESIEGIPPTIAIDQSRPVRTSRSTVGT
                     MTELHDHFKLLFSKIAVLHCRGCDRVVERDSAQSVFTKLSSLPEGTPVVITFPLAPSS
                     LPWDEVRGGLQQAGFHRLLVEQDIRDLVEIGMAPPGDAALEVVADRVAYRPGNKKRIS
                     DSLEQAFHYGKGRLNLFLSQENWRREPFSNHLHCPHCDISYRDPVANLFSFNSPLGAC
                     D"
BASE COUNT          644 a          777 c          718 g          636 t
ORIGIN      
        1 cgattggtaa ctcctcagat cgacccgtgg ctttggctga gttaatcggg atcttggtga
       61 acgacggcgt gcggcggccc aatacggagc tgagcaacat tcatttcgcc cggggtacgc
      121 cctacgagac tgtcttcgag cgcaagacgg agccaggaca gcaagtgctt gtcccggagg
      181 tggctcgggc ggtcagaaag gcgatggccg gggtggtgga gagcggaacc gcgcggcgac
      241 tcgctggtgt ttttcggcta tccgacggaa aggtgatcac ggttgggggt aaaaccggtt
      301 ccggtgacaa ccgaattgag acttttaatc gatggggcaa cgtaacttct tcgcgggcga
      361 cgaatagaac cgccgctttc gttttttata tcggaaatcg ctactttggt gtcatcaccg
      421 cgtatgtgca gggacgtgaa gctgagcact atcacttcac cagctctctg cctgtaactc
      481 ttctcaagtt actagcgccg accgtcatcg cgaagctgga taaaaatgtc gtccaaccac
      541 ctcccgcccc gggcgacgcg attcagaacg acggaagtac acgcaaaatc aatttcaaga
      601 tttcagccaa gtccccttcg gaagctcgct ttcgagatca caagcggcca atttcaattg
      661 atctgaaatt aggtaccacg ccgatttccg aacaactcga tctgtagacg cggcgtataa
      721 ccgaagccgt actctttgca cgcctcgatg agccacttcg ttttatctcg tagctctctc
      781 ctcgtcttgc cctgtcccat gatcaacaca cgggggcggt ccacgttttc caacctccca
      841 agaatctctt cgatctcatc aaaatcctct ctctgatcca ccacgaactt gagttggtag
      901 tcgtacgtgt caatgaattg ctgcatcacc tcgaagttga gccgcgccgt ctcatgcatt
      961 ttggcaaatt taccctttgc ccggcgccag ggggtagaat tggcgagctt aggactcatg
     1021 gaaacaagat cgcaggcgac cggcttgaat agggtcgctg cggtctcaat ggtaatgtga
     1081 gatcccaagg gcttaatccc cgcggtcagt tcttcaattt ctgccgctag aaacggctca
     1141 ccccccgtca ccacaatatg ccggctcggg tattttgcca cttcgcttag gatttccccg
     1201 acgctccact tttttccctc cggcgcccat gatgtatagg gagtatcgca ccagacacag
     1261 cgtaagttgc aacccgacgt gcgaataaaa accgaaggca tgccgacgaa cctgccttcg
     1321 ccctgtatgg aatggaagat ttccgcaatc tgcacgaagc tacattaaca aagcattcat
     1381 agctggggaa gttaccccgt gagatcgcca gatactcagg ttgactcgac ctctaccttc
     1441 gcttgtgata gaaataaaag gttcgatctc gcggaaaata acggagctat taccttcgcc
     1501 gatttcatcg cgatcaatga gccaagataa agaagcgttg ttggcagagg aacggaaact
     1561 tcgccggctg agcagggcga tggacatcgc ggccatatta ctctgggaag tcgacctcac
     1621 cttagaggaa gcccaggaga tcgtaaccca cgccaagcac accgcgctcc agctttttcc
     1681 ggacaaggaa gaaacctttg atttgatcta cggtcctcgc ttccgacgca tcctgacgga
     1741 aaagtatcaa ctgcaataat gctcaaatgt acgttatgag tgtagataat tcgcttctga
     1801 aacaccttca ttcaaggccg atcaaaaagg cctcagatgc aaggcgcgcg aagatcgacg
     1861 agcggaggcg tacataaact agtacgtcga gcgagtcggg caagcgcaac gaagcagatg
     1921 ggcctttttc agcggacttg agtcatgagc cagaattgga tccatatccg gggcgccaaa
     1981 cagaacaacc tcaagaatct tgacctgcaa atccccttaa atgccttaac ggtcatcacg
     2041 ggcgtgagtg ggtcgggaaa atccacgttg gccttcgata ttctctacgc cgaaggccag
     2101 cgccggtacg tcgaaagctt ttctgcttat gcgcggcagt ttctcgaccg gatggacaag
     2161 cccgatgtgg agagcattga gggcattccg ccgacgatcg ccatcgatca gagccggccc
     2221 gtcagaacct cgcgttccac cgtcggcacg atgaccgagc tccatgacca tttcaagctg
     2281 cttttctcca agatcgccgt gcttcattgc cgcggctgcg atcgggtcgt cgagcgcgac
     2341 agcgctcaat cggtttttac gaaactttct tctctaccgg aaggcacgcc agtcgttatc
     2401 acttttccgc tcgctccttc ttcgctgcct tgggacgaag taaggggcgg tctacagcaa
     2461 gccggcttcc accggctgct tgttgagcaa gacatccgcg accttgttga aatcggcatg
     2521 gcgccgccgg gcgatgccgc gttggaagtt gtcgccgatc gagtcgccta ccgccccggc
     2581 aacaagaaaa gaatctcgga ttctttagag caagcctttc actacggcaa gggtcggttg
     2641 aatcttttct tgtcgcaaga gaattggcgg cgggaaccct tcagcaatca tctccattgc
     2701 ccgcactgtg acatctctta tcgtgatccc gtggctaatc tcttctcgtt taacagcccg
     2761 ttgggagctt gcgat
//
LOCUS       DASSWZ010000257         5329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_250140, whole genome shotgun sequence.
ACCESSION   DASSWZ010000257 DASSWZ010000000
VERSION     DASSWZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250140"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..2360)
                     /locus_tag="VFN58_06190"
     CDS             complement(594..2360)
                     /locus_tag="VFN58_06190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET8917614.1"
                     /translation="MARTGVVSGKDWQPQSAIAKLPDRYLIHTKGEDRELVCTESPTM
                     AVRIERGKTVPANTARRAPSGTIYLDGAAEGGPFLDAEKAVFNLDHHEGCVRSFTLAT
                     CEQAMIIVRKGLDLQKRDWTIHANDPDLDTVLAIWVLLNHMRLSDADAEIRRKAMPLV
                     RLEGTIDAHGLEMQEICAFPPQLQASVFAELERLRSNEVALKKDGKWHEIDFLEYTAD
                     VLRAIDKIIYSSHDFEDLLEVEELARVEIGESQLAIVCRGDVEIYEIERQLRRLHGKR
                     LGVIILQKDSKTYTLRQVDAFLPGTLDGAYDLLNLIDPAAGNRGSGNRWGGSTEIGGS
                     PRATGTALTAQEIADALAQAYRRPTTARRLTDLGVALLESGVVMAGSIAAAYLQGMFR
                     NPVGSIKIYLENQAATYAAMLAVLSAALFLRAIRNGRKLFGLRMSAGMGWLGFLPPAL
                     LGGIYGGAWILRVPDTITGSYVDNYWVRLAVAVILPVVAEGLFRGLVHGRLAQHFRIQ
                     HSGGPWFLSWPVITSSALYALWTSLPFLPFYSSGVGLTFAAALLFGISSGMARERSES
                     LLPCIILHWSCLLAFVVWEWLG"
     gene            complement(2602..2916)
                     /locus_tag="VFN58_06195"
     CDS             complement(2602..2916)
                     /locus_tag="VFN58_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917615.1"
                     /translation="MRWRNFFVDAMGMTVVQKRKDNSIDLSDGSVNIAILPLAANRSR
                     RAGLDHIGFSAEDGQEQIRRMEAAGATEESTRSPYEKKFKGPEGIIIDVGRWPGSAPL
                     EK"
     gene            complement(3211..3555)
                     /locus_tag="VFN58_06200"
     CDS             complement(3211..3555)
                     /locus_tag="VFN58_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017163011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917616.1"
                     /translation="MQIHYLEIVTPDVDASVALYSKIHGISFGAADESLGGARTAKLA
                     NGGTLGIRAPLRDTETPVVRPYFLVHDIEAAVAAAGQSGAKIAMQPTEIPGRGKFAIL
                     IQGGIESGLWQV"
     gene            complement(4024..5004)
                     /locus_tag="VFN58_06205"
     CDS             complement(4024..5004)
                     /locus_tag="VFN58_06205"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917617.1"
                     /translation="MKNKFMWIIALLLLSIGTFAEAQQAKKVQRIGYLTSNTSSAELP
                     RFDAFRQGLRDLGHVEGQNIAIEYRHAEGKFDRLPGFAAELVGLKVDVLVGVTTNAVV
                     AAKEATRTIPIVFIGVSDPVAAELVDSLARPGGNLTGFTNIAPVLSGKRLELLKETVP
                     KLSRVAVLWDPRSPGSEPQWKESQLAARELGLQLHSLEVSSADKYEGAFKEATKARSG
                     ALAVTLNPVSNSNQKPIADLAIKNRLPTMCARGDYVDNGCLMSYGPSYVAEGRDAARY
                     VDKILKGTKPADLPVEQPMKFEFVINLQTAKQIGLTIPPNVLARGDKVIK"
BASE COUNT         1222 a         1509 c         1408 g         1190 t
ORIGIN      
        1 atactgatca cgacaatctc agaatttgca gcgcgcgtgc agacgcattg tgacgaagaa
       61 cacgtgactg ctaatcgaca ggtgtcgaaa tgtcgacttt agctcggacg agcaatcccc
      121 agttttttat ccgtgcttga agagttgtgc gctttatcct gagtcgagcg gtggtacttg
      181 tgggcccgcc aatgatccaa tttatgtcct tgaaaagtga cagtcaggat ccggagtttc
      241 agaaagaata tctaaagctc gccggcgaag aatcgacccg gtcatgactc aagttgctag
      301 gataccgaaa gtgatgccag cggccaggtc gccacgagaa acccggaatc ggcgataagc
      361 aaggccggct tggcaccgcc caaccaagca ccggcgcacg ctgccaccgc ttcaccctcg
      421 ttggttaccc ctacggtcgt aaagtcctta tcttccgtga tcagttactg cgcgtcatga
      481 atctggtcgt ccggaaggta tgcgacgaaa ttgattcccg attccttgag cgcttcatgc
      541 acgataaccg cgtattgctt catggatttt ccatcggtgg ctaagctctc atactatcca
      601 agccattccc acacaacgaa cgccagcaaa caggaccaat gcagaataat gcagggcaac
      661 aggctctcgg aacgctcccg ggccatgccc gagctgatgc caaagagaag ggcggcggcg
      721 aaagtcaaac caacacctga cgaataaaac ggaagaaacg gcagcgaggt ccaaagcgcg
      781 tacagcgcgc tcgaggtgat gaccggccag gagagaaacc agggacctcc gctatgctgg
      841 atacggaaat gctgagccaa cctgccgtgc acgagcccgc ggaataatcc ttcggccacc
      901 acggggagga tgactgctac cgcgagtcgg acccaataat tgtcgacata gcttcccgtt
      961 atcgtatccg gtactctaag gatccaagcg ccgccatata tgccgccgag caatgccggc
     1021 ggaagaaagc cgagccaacc cattccagca gacatgcgta gaccgaaaag ctttcgtcca
     1081 tttcgaatcg cgcggagaaa cagcgcggcg ctgaggacgg cgagcatggc tgcataggtc
     1141 gccgcttgat tctcgaggta gatcttgatc gaccctaccg ggttgcgaaa cattccctgg
     1201 aggtacgcgg ccgcaataga gcccgccata acgacgccgc tttccaaaag cgcgactccg
     1261 agatcggtca aacgccgcgc ggtcgtcggc cggcgatagg cctgggccag ggcatcggcg
     1321 atctcttgcg ccgtgagggc agttcccgta gcccgcggcg agccgccgat ttccgtcgag
     1381 ccgccccagc ggttgccgga gccgcgattg ccggccgcgg ggtcgatcaa gttcagcaga
     1441 tcgtatgcgc catcgagcgt ccccggtaag aatgcatcca cctggcgcaa tgtataggtc
     1501 ttcgaatctt tctgcaggat gatgacgccg agacgctttc cgtgcagccg gcgcagctgg
     1561 cgctcgattt cataaatttc tacatcgcct cgacaaacga tcgccagctg gctttcgccg
     1621 atctcgacgc gcgcgagttc ctcgacttcg agcaggtctt cgaagtcatg cgaggaataa
     1681 ataatcttgt cgatggcgcg caaaacatcg gctgtatatt ccaggaagtc gatttcgtgc
     1741 cacttgccgt ctttcttcaa ggcgacttcg ttgctccgta gccgctccaa ctcggcgaag
     1801 acagacgcct gcagctgcgg aggaaacgca caaatttcct gcatttcgag tccgtgagcg
     1861 tcgatggtgc cttcaaggcg caccagaggc atcgccttgc ggcggatttc cgcgtcggca
     1921 tcgctcaacc gcatgtgatt caacaacacc cagatggcga ggacagtatc gagatcggga
     1981 tcgttggcgt ggatggtcca atctcgcttc tggaggtcca aacctttgcg cacgatgatc
     2041 atggcctgct cacacgtggc cagagtgaaa gatcggacac aaccctcatg gtgatctaaa
     2101 ttgaagaccg ccttttctgc gtcaagaaag ggccctcctt cagctgctcc atcgagatag
     2161 atggtccccg acggcgctct tcgggcggta ttggcgggta ccgtcttgcc ccgctcgatc
     2221 cgaaccgcca tggtgggcga ttcggtgcag accagttcgc ggtcttcgcc tttagtgtgt
     2281 atcaagtagc gatcgggaag tttggcgatg gccgactgcg gctgccagtc ttttccggat
     2341 acgaccccag ttcgcgccat tatgcgaaag gctccatgcg ggaattgtag ctaagccgcc
     2401 gtcgaagtga caagcggttt agcgtcacgg ttttccgctg ccgggctcgg ccaccgccgg
     2461 cgagcgctca aatgcgctcc cgttccattc accagggccg cccccgatgc aaaggcgatc
     2521 gcgttggcca cctatggtta ggcagccttc tccaccattg gatttgtccc gttttctttc
     2581 actgacaatc gcagcacgat cctatttttc cagaggtgcc gagcctggcc aacggccgac
     2641 atcgataata atgccctcag gacctttaaa ctttttttcg taaggcgacc gcgtactctc
     2701 ttccgtcgcg ccggccgctt ccatcctgcg aatctgttcc tgaccgtcct ctgcagaaaa
     2761 accaatatgg tcgagaccag ctctgcggct cctatttgcg gccagtggaa gaatcgcaat
     2821 atttactgaa ccatcactca aatcgatgga gttgtccttc cgcttctgaa ccactgtcat
     2881 gcccatagca tcgacgaaaa aatttcgcca tcgcatcgac atcctcggcg cggtaagaaa
     2941 tatgcctcac ttcagccatg gtccgcccct tgatcaattt gacagtcagg cttcactatt
     3001 gatgacttgc aatgcgaaca cccaaaattt accggatttg ctagagggag aaatcagttc
     3061 aattagatgg ttaacgacca agctcagcga ccgccgccgg aaacgcccgg tcggctgcaa
     3121 cagtcgctga caaattatcc gaaccgtccg actgcaaagg ggggcggcgg ttcgctgcag
     3181 cgctcttgtt atgtgacgaa acggccacaa ttagacctgc cacaatccag attcaatgcc
     3241 tccttgaatc aaaatggcga atttaccgcg tcctgggatc tcagttggct gcattgcgat
     3301 ttttgcgccg gattggcctg cggcggcgac ggctgcctca atatcatgga caaggaagta
     3361 aggacgcacc accggggtct cggtatctcg cagtggagct cgaatcccca gcgttccgcc
     3421 gttcgccagt ttggcagtgc gtgcgccccc tagactctca tcggcggcgc cgaaagaaat
     3481 gccatgaatc ttggagtata gcgcaacaga cgcgtcaaca tccggtgtca ctatttcgag
     3541 gtaatgaatt tgcattgtat tccctttctc agtgttcgtg tcctcaaccg ccaattggtc
     3601 gccagacaat tcgttcgtgc tttccgccca gactatcatg atttggcgaa caaccaatgt
     3661 gtaggatggc tgtccacggc attgcaataa aggcaatacc agcatggata agagattacg
     3721 ccgattgatc ctctgcatag tccttcgtca cataaccagt gagtatactg accaatatat
     3781 taccgatttg aagaaatcac ggctccgcga gatttacctg tatgttcaac gcgttgtcaa
     3841 tcatttttcg ttcttgttca ttccaatata cctgcaagcc ggagccgcaa gatgcaggat
     3901 tgaattgaac gacacaacag tcgcgtcttg taggattaag tcgtaggacg caacagtcgc
     3961 gtccaggatt gaattgtagg agaattcagt tcaatcctgg caacgcagct tcgttttctt
     4021 cgatcatttg atcaccttat ccccgcgcgc caggacgttc ggcggaattg tcaggccgat
     4081 ctgcttcgcc gtttgcagat tgatcacaaa ttcaaacttc atcggctgct ctacgggaag
     4141 atcggccggc ttggtgccct tcaagatctt gtcgacatat cgagcggcgt ctcgcccttc
     4201 ggcaacgtaa ctgggtccgt aagacatcag gcaaccgttg tccacataat ctccccgagc
     4261 gcacatcgtc ggcagccgat tttttatcgc gaggtccgct atcggttttt gattagaatt
     4321 ggacaccggg ttcagagtca cggcgagagc gccactgcgt gccttggttg cctctttgaa
     4381 cgcgccctcg tacttgtcgg cactgcttac ctccagggaa tgaagctgca gacctagttc
     4441 tcgtgccgcg agttggcttt ctttccattg tggttcagag cctggagatc gtggatccca
     4501 cagcaccgca acgcgggaga gctttggaac ggtttccttg agcaactcca gtcgtttgcc
     4561 agacagcacc ggcgcaatgt tggtgaaccc tgtgaggttc cccccaggtc gcgctaggct
     4621 gtcaaccagc tcagccgcaa caggatcaga cacgccaata aaaacgatgg ggatcgtcct
     4681 tgtcgcctcc ttggcagcta caacagcatt tgtcgtcacc ccgacaagca catcaacctt
     4741 gagaccgacg agctcggcag caaagccggg gagccgatcg aacttcccct ccgcatgtcg
     4801 gtactcaatg gcaatgttct gcccctcaac atggccgaga tcgcgtagcc cttgccggaa
     4861 tgcatcgaac ctaggcagtt cggcagaaga agtgttggaa gttagatacc ctatccgctg
     4921 aactttcttc gcctgctggg cttcggcgaa ggtacctatg ctgagaagga gaagcgcgat
     4981 tatccacata aatttatttt tcatgctttt aataaattcc gcaatggata tgccacaaaa
     5041 ataagcctgt aaaaatcgca gtttggcgat gaaggaaggc aaggggagcg tctcattctg
     5101 gatacagtgg cgtatgaaac ttgattcact cacggcggga aggattgacc aggttttagg
     5161 ccgtttaaca agcattgaaa cctcgtgcgc ttacctttgc caacgttcag cctcgaacca
     5221 atacccaggc cggcaagaga agcttcgaga tcgcggcaaa gattgtttac cgaaacaatg
     5281 gacgtatggg gagtccgagg ggtggcagca tggattgcac gatggtcgc
//
LOCUS       DASSWZ010000258         6239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_193608, whole genome shotgun sequence.
ACCESSION   DASSWZ010000258 DASSWZ010000000
VERSION     DASSWZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193608"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /gene="leuS"
                     /locus_tag="VFN58_06210"
     CDS             <1..1955
                     /gene="leuS"
                     /locus_tag="VFN58_06210"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET8917618.1"
                     /translation="CWRCESEVSKRDIDGWFFKITAYADELLDWCDRLTGWPERVITM
                     QKNWIGRSEGAEFDLPVVGHRDLKVRVFTTRPDTGFGMTYAVLAPEHPLVDRLVTEPA
                     EQAKVERFRAEVLKQSEQERVAEDRPKHGLRLSAHVINLFNGREIPLFIADYVLMIYG
                     TGAIMAVPGEDQRDWDFAKTHGLDIIETVSRPANWTGGAYTGDGIKINSGFLNGLTVA
                     DAKRRAIDWLVERGFGEARVNYRLRDWGISRQRYWGAPIPVIYCDACGMVPEKEENLP
                     VVLPHDVEISGKGGSPLAGHAAFVSAACPRCGGQARRETDTMDTFVESSWYFLRYCSP
                     HFEGGIVEREAAAYWMPVDQYIGGIEHAVLHLLYARFYTKVLRDLGLVKVDEPFNALL
                     SQGMVVKDGAKMSKSKGNVVSDDVIRDKFGADTGRLFELFAGPPEKDLEWSDQGVEGA
                     YRFLTRLWRFTFQHRKDLIAARSDFSQNDLPGDLRNLYRLIHRTIKKVTEDIESRFHF
                     NTAIAAIMELFNALSASVQDETKLGIGTPVIKAGLETIITMLYPFVPHIASELWESLE
                     HRGSLDAVTWPKYSKEALEEEQLLIVVQVNGKVRGRITVAAEVDPKEIESEALQDQKV
                     KSFLDGKKIQKVIHVPRRLVNIVLEG"
     gene            1958..2566
                     /locus_tag="VFN58_06215"
     CDS             1958..2566
                     /locus_tag="VFN58_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HET8917619.1"
                     /translation="MGQKWVFQSLIGPCTYLAFLSLLGCGYQFAGRSDLFPKDIHSVY
                     VEPFLNRTRDISLQYELATALRSELYRRGELRLVDQADQADAILSGVVRSFNSLTWSV
                     NQVDEVLQYEATLIVDVTFRRREPSEILWRGQNIRLTEIYAGQRAAVVVTSPAFQNQT
                     LNTTDVRNLTDIQLTESANQLMRENLLERFARELHQRLMEMF"
     gene            complement(2618..2926)
                     /gene="rpsT"
                     /locus_tag="VFN58_06220"
     CDS             complement(2618..2926)
                     /gene="rpsT"
                     /locus_tag="VFN58_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET8917620.1"
                     /translation="MLERLHSFEEDYLALHPSVIKRHRQSEKRRARNVESKTKLRTLI
                     KKARQAIESKNPDSVSAQIKAVNQALGKAVSKGTITRNTASRWLSRLSRSANSTAMAS
                     "
     gene            3814..4068
                     /locus_tag="VFN58_06225"
     CDS             3814..4068
                     /locus_tag="VFN58_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917621.1"
                     /translation="MGKRIVLLSGTTLFALMLTFAGCGKREEPAPPPPPPPAPAPAAP
                     APAEPPAPSGEMKAPADQPSGGAPMEKMPMEKKDEGTKTK"
     gene            4164..4889
                     /gene="tatC"
                     /locus_tag="VFN58_06230"
     CDS             4164..4889
                     /gene="tatC"
                     /locus_tag="VFN58_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET8917622.1"
                     /translation="MATDSYMTLTEHLEELRWCLLKSLAAIILASGLGYFFSDAIFKI
                     MVAPLRQSLRPGQSLIGTAVTEAFFVEIKVALAAGIFFSCPVIFYQIWRFIRPGLSGS
                     EKRLVLPFVLCATFFFLGGAFFCYRIVLPVAFQYFIEQYGTIGVTPAIRIGEYFTFFF
                     RMILAFGVTFELPVFTFFLVRLGVWNYRLMLSTFRYAIVAIFIVAAILTPTPDIINQS
                     LLAGPMLLLYLVSIGVAYLWRRD"
     gene            4927..>6239
                     /locus_tag="VFN58_06235"
     CDS             4927..>6239
                     /locus_tag="VFN58_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET8917623.1"
                     /translation="MILLVITISCLSLLFAVYLARYVLRHDMGTDKMQEISNAIKEGA
                     EAFLSRQNRTIILFASVLAVLIFLLYAFVRTPSPADPVLPVQMAFWTTFSFVLGALCS
                     VIAGYVGMWVSIRANIRTAAAVRSSLNEALKIAMRGGAVSGLFVVAMSLLGVGGLYAL
                     LDYLGHDPQKIPFTIVGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGLYPFFGLKGILFPLVA
                     RAFGLIASIIGVIIVKSNEQEDPMVALNRGYYVTSALAILGFFCAARWMLAVDSAQHP
                     EAISAWFYFFLCGVIGIAMAQAFVYITQYYTEYKYRPVKSIVEASQTGPATNIIAGMA
                     VGLECTAIPVGVISVAILASYYLGNLSGVPHAGLFGTAVATMGMLG"
BASE COUNT         1354 a         1536 c         1756 g         1593 t
ORIGIN      
        1 gctgttggcg gtgcgagtcc gaggtcagca agcgtgacat cgacggctgg ttcttcaaga
       61 tcaccgctta cgcagatgaa ttgttggatt ggtgcgaccg actcacaggc tggcccgagc
      121 gtgtcatcac gatgcagaaa aactggatcg ggcgcagcga gggcgcggaa ttcgacctgc
      181 cggtagtggg ccaccgggac ctgaaggttc gggtgtttac cacacggccc gacactggat
      241 tcggcatgac ctacgctgta ctggcacccg aacatccgct ggtggaccga ttggtgaccg
      301 aacctgctga gcaggcgaag gtggaacggt tccgcgctga agtgctcaag cagtctgagc
      361 aggaacgcgt ggccgaagac cggcccaagc acggcctccg actctctgcg cacgtgatca
      421 atctctttaa cggaagggag ataccgctgt ttatcgcgga ctatgtgctc atgatttacg
      481 gcaccggcgc tatcatggct gtgcccggag aggaccagcg ggactgggat ttcgcgaaga
      541 cgcacgggct cgacatcatc gagacggtca gccggccggc gaactggacc ggcggggcgt
      601 acaccggtga cggcatcaaa atcaattcgg gtttcctcaa tggattgacg gtggccgatg
      661 ccaaacggcg ggcgatcgac tggctagtcg agcggggttt cggagaagct agggtcaact
      721 atcgtctgcg tgactgggga ataagccgcc agcgctattg gggtgcgccg attccagtga
      781 tctactgcga cgcctgtggc atggtgcccg aaaaagagga aaatctcccg gtcgtcttgc
      841 cgcacgatgt cgaaatcagc ggcaagggag gctcaccgct ggccggacat gccgcgttcg
      901 tcagcgccgc ctgcccccgt tgcggcggcc aggcgcggcg cgagactgac acgatggaca
      961 ctttcgttga gtcatcgtgg tatttcctcc gctactgttc acctcacttt gagggtggca
     1021 tagtcgagcg agaggcggcg gcgtactgga tgccagtgga tcaatacatt ggtggtatcg
     1081 agcatgctgt gctccacctc ctctacgcgc ggttttacac caaggtactg cgcgatctcg
     1141 ggctcgtgaa ggtggacgag cccttcaacg cgcttctttc ccaaggcatg gttgtcaagg
     1201 acggtgccaa gatgtcaaag tctaagggca acgtcgtctc cgacgacgta atccgcgaca
     1261 aatttggggc tgacaccggc cgattgtttg agctttttgc gggtcctccg gaaaaggatt
     1321 tggaatggag cgatcaagga gtggaggggg cctataggtt tcttacgcgt ttgtggcgtt
     1381 tcaccttcca gcatcgcaag gatttgatcg ccgcgaggtc cgatttttcg caaaacgatc
     1441 ttcctggcga tcttaggaat ctgtaccggt tgattcatag gaccattaag aaggtcacag
     1501 aagacatcga atcgagattt cactttaata cggcgattgc cgccatcatg gaactcttca
     1561 acgctctcag cgccagcgtc caagatgaga cgaagttagg aattgggacg ccggtcatca
     1621 aagcagggtt agaaacaatt atcactatgc tttacccctt tgtgcctcat atcgcgagtg
     1681 agctatggga gtctctagaa catcgaggca gtctggatgc cgttacttgg ccgaaatact
     1741 cgaaagaggc gctcgaagaa gaacagctgc tgattgtggt gcaagttaac gggaaggtac
     1801 gcggcaggat taccgttgct gccgaggtag atccaaagga aattgagtcg gaggcattgc
     1861 aggatcagaa agtaaagagt tttctagatg gcaaaaaaat acaaaaggtc attcacgtcc
     1921 cacgtcggct tgtgaatatc gtattggagg gttgaggatg ggccaaaaat gggtatttca
     1981 atctcttatc gggccgtgca cttatctcgc gttcctatcc ctgctcggtt gcggctatca
     2041 attcgcgggg cggagcgatc tttttcccaa agacatccac tccgtatacg tggaaccgtt
     2101 tctgaatcga acgcgggata ttagcctgca atatgagtta gccacggcgc tgcgaagcga
     2161 gttgtatcgc cggggcgaac tccgactcgt ggaccaagcg gatcaggcgg atgcgatctt
     2221 gagcggggtt gtgcgctctt tcaatagcct aacgtggtcg gtaaaccaag ttgatgaggt
     2281 gctccaatac gaagctacat tgatcgtcga cgtaacattt cgccgtcgag agcccagtga
     2341 aatattgtgg cggggtcaga atattcggct caccgagatc tatgccggtc agcgggccgc
     2401 ggttgtagtt acatcgccgg catttcaaaa ccaaacgctg aataccacgg acgtgcggaa
     2461 cttgacggat atacagctca ccgaatcagc caatcaattg atgcgggaaa atctattgga
     2521 gcgtttcgcc cgcgaactcc accagagatt gatggaaatg ttttgatgac caacgtagat
     2581 cgttcacgag tcgagtctgc gccgagaggg aaacccgcta cgaggccatt gccgtggagt
     2641 tagctgaccg cgacaaccgc gataaccagc gagaagctgt attacgcgtg atcgttcctt
     2701 tgctgacagc tttgcctaag gcttgattga ccgctttgat ctgagcagaa acactatctg
     2761 gatttttcga ttcgatcgcc tgccgtgcct ttttgatcaa agttcttaac ttggtcttcg
     2821 attccacgtt tcttgcccgt ctcttttcgc tctggcgatg ccgtttgatg accgatggat
     2881 ggagtgccaa ataatcctcc tcaaatgaat gcagcctttc taacatctag ctccgaagga
     2941 gtcaatgtga cgaaaaaacg ggacgatgga aaccagattc tgcactgcga tttttttgca
     3001 atgtgtgggt cgacgttcag ctttcccacg cgatgcgagg tgtgacgaaa cgttcggtcg
     3061 cttgataact ccgttttgcg agtctaacgc gggtcttctg tggtgtctta ggtgaaactg
     3121 tttctctcca aggtccgaat ggaaatagat tcgtctaatt aaccagaact gccatcgaat
     3181 ccatgttttg aacgaactta ataactgcgc taacttaaag agaatttatt ggcgtgaaat
     3241 tgggcatttc gctgagctgc gctatttttg gccctctgct tgaccccctt aattacttat
     3301 caacaagtta ctaacagttc ttgtgggctc ggattttacg ctgtatctct agctttgcca
     3361 gtgatcgaag gtacccccct cagctctgca ggggactctg gagtctagca tttgctggcg
     3421 aaaaaacttt caaactgcga actgctccag ctcgacagag gggagatagc aatcaaggtg
     3481 agacacatgc aatgcaaacg caagaatgca aatatctcgc ggatcataac tcaaccctct
     3541 ggctctgccg gagctataga gcttcgcttg cattatctag aaatctttcg gctttgagaa
     3601 tttgcttaaa atcctttgga ccctcaggtc tccgcaaaac tagaatcggg ttgtgcgact
     3661 ggcgtccaac cgcaaaacct cacagaccga aatggtagca ttcgcgtttg attatccata
     3721 gatccccttg caaggaagcg ggacttgtgc aataaaaaat tatctagcaa aacggttttt
     3781 cagagatgtg tcgggaaagg aggtgatctc aggatgggaa aaagaatcgt tttattgtcg
     3841 ggaacaaccc tcttcgcctt gatgctaact tttgcagggt gcggtaaaag ggaggagcca
     3901 gctccacctc ccccgccgcc gccagcgcct gctccagcag cgccagcgcc ggcagaaccg
     3961 ccggcaccta gtggtgaaat gaaggccccc gctgaccaac cttcgggcgg agcgccgatg
     4021 gaaaagatgc cgatggaaaa gaaagacgag ggaactaaaa ccaagtaaag gtgaattaac
     4081 tttaccgcgg ttttgataac cgaagaaggg gaagggtgtg tatccttccc cttttttgcg
     4141 atattggtcc ggggtagtgg cttttggcga cagattccta catgacgtta acggagcacc
     4201 tcgaagagct ccggtggtgt ttgctcaagt ctttggccgc gataatctta gcttctggac
     4261 tcggttattt tttttcggac gcgattttca agataatggt ggccccgctt cgacaaagcc
     4321 ttcgtcctgg gcaaagtttg attgggacgg cagtcaccga agcatttttt gttgaaataa
     4381 aggtagccct agctgccgga atttttttct cctgtccggt tattttctac cagatctggc
     4441 gctttattag accgggtctc tcgggtagcg agaagaggct ggtcctgccg tttgtcttat
     4501 gtgcaacgtt tttttttctg gggggagctt ttttttgcta ccgaatcgtt cttcccgttg
     4561 cgtttcagta tttcatcgag caatacggca cgattggggt aacaccggca atacgtattg
     4621 gcgagtattt cacctttttt tttcgtatga tcctagcgtt tggggtgacg tttgagttgc
     4681 cggttttcac attttttttg gtgcgcttgg gcgtctggaa ttaccgtctc atgctcagta
     4741 ccttccggta tgccattgtc gcgatcttta ttgtggccgc tatcttgacg ccgacgccag
     4801 atattattaa tcaatctctc ctggccggtc cgatgctctt gctttatcta gtgagtatcg
     4861 gggtagccta tctgtggcgg agggattaag gaaattattt tactgaagaa gggatgatcg
     4921 accatcatga tcttgctggt aatcaccatt agttgtttat ctctgttgtt tgccgtgtac
     4981 cttgcccgtt atgtgctccg gcacgatatg ggcaccgata aaatgcagga gatctccaat
     5041 gcgatcaaag aaggcgcgga ggcatttctg tcacgtcaga accggacgat cattcttttt
     5101 gccagtgtgc tagcggttct gattttcttg ctctatgctt ttgtccggac tccaagtccg
     5161 gccgaccccg tgctgccggt acaaatggct ttctggacta cgttttcctt tgtgctcggc
     5221 gccttgtgtt cagtcatcgc cggttacgtc ggcatgtggg tgtcgatccg cgccaatatt
     5281 cgaacggcgg cagcggttcg atcgagcctg aacgaggcgc tgaagatcgc gatgcggggt
     5341 ggggcggtgt ccgggctttt tgtcgtggcg atgagcttgc tgggagtcgg tggtctttac
     5401 gccttgctcg attatttggg acacgatccg caaaagattc cctttacaat cgtgggttac
     5461 ggttttggcg cttcctttgt tgctctgttt gcgcaactcg gcggaggaat ctataccaag
     5521 gccgccgatg tcggcgcgga tctcgtcggc aaggtggaag caggaatccc agaagatgat
     5581 ccgcgcaacc cggcggttat tgcagatctc gtcggcgaca atgtgggtga ttgcgccggt
     5641 cgcggagccg acctgttcga atccacggcg gcggaaaata tcggcgctat gattcttgga
     5701 gtcgggctgt atccgttctt cggtttgaag gggattctct ttccgctcgt cgctcgggct
     5761 tttgggctca tcgcatcgat tatcggcgtc ataattgtaa aaagtaacga gcaggaagat
     5821 ccgatggtag cgctaaatcg cggttattac gtcactagtg ctctggccat tctcgggttc
     5881 ttctgcgccg cccggtggat gcttgccgtc gattccgcgc agcatccgga agcaatctcg
     5941 gcgtggtttt acttttttct ttgcggggtc atcggtatcg ctatggcgca agcatttgtc
     6001 tatatcacgc agtattatac agaatacaaa taccgtccgg ttaagtcgat cgtagaagcc
     6061 tcccagaccg gcccggcgac caatatcatt gccggtatgg ccgtaggact ggagtgcacg
     6121 gcgattcccg tcggtgtgat ctccgtcgcg atccttgctt cctattactt gggcaatctg
     6181 agcggcgtgc cgcatgcggg tctttttggc accgccgtcg cgacgatggg catgctcgg
//
LOCUS       DASSWZ010000259         5384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_434277, whole genome shotgun sequence.
ACCESSION   DASSWZ010000259 DASSWZ010000000
VERSION     DASSWZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434277"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..539)
                     /locus_tag="VFN58_06240"
     CDS             complement(93..539)
                     /locus_tag="VFN58_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8917624.1"
                     /translation="MLEAKNLSTIEKVIFLKSVDIFEHATVEQLGRIAGLMEEVNFEA
                     RETIYNEGDAGDALYVILSGRVALERNDSTIRELNELKEKEAFGTLEVLDFHPRAANA
                     KAIDQVRALKLNGQEFHDLLSVDFEMVEAVLRMLCGVVRRVLTLVP"
     gene            complement(541..3327)
                     /locus_tag="VFN58_06245"
     CDS             complement(541..3327)
                     /locus_tag="VFN58_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET8917625.1"
                     /translation="MNHFSLPNTLSRFLDIRPGEARRVGIMVSLLFFLLAANNVIKVV
                     RDSFFLSHFPISYLAYVYLLAAVLAGVIIAVYTRYTVRVPLYRLILASNAFIISNVIM
                     FWFLIVFFNFAWAIFAFYIWSAIVGVLAVAQFWTFAGLIFTSREAKRLFGIFNAGGSL
                     GAILGGFGSGWAVNIMRSHELLWLIGALFAGAFAVVWLAGSKLSELQTVGLTEIPASK
                     ETNASQQTSALGAIRASRYLQLIAGGIFVSVAVSTLIDFQFKAAAKTAYDSQHALTAF
                     FGSYYAWVAIITLFSQVLLTGRFLTAFGLIPSLLLLPASLFAGSLGILVWPGLFSTTA
                     TRLTDTVLRSSVNQSGMEILYLPLSASLRKQIQTFLDVVLQRLGDGAAGLIVLFYALF
                     MMQSEPASLPYVSLALIIIWAMFIFVLRRGYLEALRTGLETQAITWEGGEINYADKQT
                     IGAVLETLQRRDERALLFGLDLAEKLDPEVIVPRLPLSLLQHPSPLVRSRSLKLFAAT
                     TDPEKLKEIVRLLEDENGEVQAEAINVVCAIRKEDAIPVMRPYLESPDPRVQRSAIEC
                     LLHHGDREIREVALATFRKMITNTQVDGAAARVEAARLIGDVDDSELRRYLTMFIRDD
                     SSMAVVRAALAAAGKIKEPTVVRDVIMQLGSPKAKSTAHQALVQYAETAVQSLREMLL
                     DSKVSHDVRLNIPSTLSKITTPTAMNALLDALNQEDGSVRYRVIIGLEELARRLPDLR
                     IERRVIETAIDAEATRYYRRFLTFFVLFGDESVDSTKNGSLLHQALLENMDRERERVL
                     RLLSLIYGPEDMTNACIALRSGSDAKKAQAIEFLDNLLSGDLKRHVFPVFDDATGVVR
                     FQQFLVFLGLESLDAKAALQELLKQDDIWLQAATLWEIGLRGLQDFRQEVQEYINSKE
                     PVLKETAELVMSRM"
     gene            complement(3738..4691)
                     /gene="panE"
                     /locus_tag="VFN58_06250"
     CDS             complement(3738..4691)
                     /gene="panE"
                     /locus_tag="VFN58_06250"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET8917626.1"
                     /translation="MRILVVGAGAVGGYFGGRLLEAGRDVTFLVRPKRAAQLAETGLV
                     IKSRFGDVMLKVPPTIQAHKIDRHFDLVLLSCKAYDLNGAIGSFAAAVGPETGILPLL
                     NGMRHLDLLDERFGAARVLGGQCLIAATVDDIGRIVHLNDSHLLTFGERHENRSPRID
                     AIVHEMSQANFESRASSVILLEMWEKWIFLATLAGITCLMRSSIGDIAAAGGKHLALD
                     LLEECRSIAASAGFAPRAEFLEPTRSTITAPGSRLTASMLRDMERHARIEADHILGDL
                     LRRGSQVLNADRSLLQLAFRHLKAYEARRDREQAAAENPAS"
     gene            complement(4749..>5384)
                     /locus_tag="VFN58_06255"
     CDS             complement(4749..>5384)
                     /locus_tag="VFN58_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET8917627.1"
                     /translation="VGAVAAVTSLQTNLTVALATALVASAAGTNAIAGYGTGVRLEYL
                     LIPVIFGLGAPLVALVGTNIGAGQKQRAVRIALVGGAVAFAISQTLGLIAALWPQAWL
                     ALFSPDPRVIETGSAYLRIVGPAYGFFGLGMALYFASQGAARLLWPLLAGFLRVIIAI
                     GGGWLALRLTGSLNWLFAALAFGLVVYGVVLTTAIISGAWFGRERTIKARG"
BASE COUNT         1310 a         1470 c         1439 g         1165 t
ORIGIN      
        1 gacgacgtgc ttcgtagtcc cgtcggtggg tctgggaaat tggcgatggt tcctcatcca
       61 ctttttcggg agcgatgtta ttgacgagaa tatcacggca ctaacgtgag aaccctacga
      121 accacaccgc acaacatccg caaaacggcc tcgaccattt cgaaatcgac tgatagaaga
      181 tcatgaaatt cctgaccgtt aagcttgagg gcccggacct gatcgatcgc tttggcgttg
      241 gcggcacgag ggtggaaatc gagaacctcc aacgtaccaa acgcttcttt ttccttaagc
      301 tcattaagct cacgaattgt actgtcgttt ctttcaagcg ccacccggcc gctcaatatc
      361 acgtacagag cgtctccagc gtcgccttca ttatagatcg tctctctcgc ttcgaaattg
      421 acttcttcca ttaatccagc gatgcggccg agctgctcta cggtggcatg ctcgaagata
      481 tcgacggact tgagaaagat gaccttctca attgtgctga ggttctttgc ttctagcaaa
      541 ctacatcctc gacatcacaa gctcggcagt ttccttgagc acaggttctt tggaattaat
      601 gtactcctga acctcttgcc ggaaatcttg aagtcctctc aagccgattt cccagagtgt
      661 ggccgcctgg agccagatgt catcctgttt taaaagctcc tgcaaggccg cctttgcatc
      721 aaggctttct agacctaaga agacaagaaa ctgttggaag cggacgacac cggtggcgtc
      781 atcaaagact gggaaaacat ggcgcttcaa atctccgctg agcaggttgt cgagaaactc
      841 gatcgcttgg gcctttttag cgtcactgcc gctgcgcagc gcgatgcagg cgttcgtcat
      901 atcttcgggt ccataaatca gggaaagcag tcgcagtacc ctttctcttt ctctgtccat
      961 attttcaagc aaggcttgat ggagcagcga gccgttcttc gttgaatcaa cactctcatc
     1021 tccaaaaagc acaaagaaag tcagaaatcg tcgatagtag cgggtggctt ctgcgtcgat
     1081 agcggtctct atgacgcgtc gttctatgcg caaatcgggc aaccggcgcg ctaactcttc
     1141 gaggccgatg atgactctgt aacgaacaga accatcttcc tgattaagtg catcgagaag
     1201 tgcgttcatc gcggtaggag tggtgatttt gctcagtgtc gaaggaatat tgagtcgcac
     1261 atcgtgagag actttggaat ccaggagcat ctctcttaaa ctttgcaccg cggtttccgc
     1321 gtattggacc aacgcctggt gtgccgtgct tttcgccttc ggagagccga gttgcataat
     1381 aacatctctc acaaccgtcg gctcctttat cttgccggca gcagctaacg ctgcgcgaac
     1441 tacggccatg gaggaatcgt cccgaatgaa catggttaag tatctacgaa gttcagaatc
     1501 gtcaacgtcg ccaatgaggc gggccgcttc tacccgagcg gctgctccat cgacttgcgt
     1561 gtttgttatc attttacgaa aggtggccag agcgacttca cgaatttccc ggtctccgtg
     1621 gtgtagcaga cattcgatcg ccgatcgctg aactcgtgga tcggggcttt ccaggtaagg
     1681 gcgcataact ggaatcgcat cttccttgcg gatggcgcag acaacgttga tcgcttcagc
     1741 ctggacctct ccattttcgt cctcgagcaa tcgcactatt tccttcaatt tttcagggtc
     1801 cgtggtcgcg gcaaaaagtt ttaacgatcg actccgaact agaggcgaag gatgctgtag
     1861 caggctgaga ggaagacgcg gaacgatgac ttccggatcc aatttttccg ccaggtcaag
     1921 cccgaataac aaagctcgct cgtcccgtct ctgtaaggtt tccagcaccg ctccgatggt
     1981 ttgcttgtcg gcatagttga tctctcctcc ctcccacgta atggcctgtg tttctagtcc
     2041 ggtccgcaat gcctcaaggt aacctctgcg caaaacaaaa ataaacatgg cccagatgat
     2101 aataagtgcg agggaaacat aggggagact ggctggctcg gactgcatca taaatagagc
     2161 gtaaaagagt acgatcagcc ccgcggcgcc gtctcccaac cgttgcaaaa caacatcaag
     2221 gaacgtctgg atctgttttc ttagactcgc agagagcgga agatagagta tctccatgcc
     2281 gctctggttc acacttgacc gcaaaactgt gtcggttagt cgcgtcgctg tcgtggagaa
     2341 aagccccggc cagaccaaaa tacccagcga tcctgcgaac agactcgcag ggaggagtaa
     2401 gagactcggg atcaagccaa aggcggttag aaacctcccg gtcaatagaa cctgactaaa
     2461 caaggtaatg atggcaaccc aggcataata ggacccgaag aaggctgtca gggcatgttg
     2521 agagtcatac gctgtttttg ccgcggcctt gaactgaaag tcgatcagag ttgagactgc
     2581 caccgagaca aagataccgc cggcaattaa ctgaagatat cgggacgctc ggatcgcgcc
     2641 cagcgcacta gtttgttggc tcgcgttcgt ttcttttgat gccgggattt ccgtcagccc
     2701 taccgtttgg agctcgctta atttgctgcc cgccaaccaa accaccgcaa aagcaccggc
     2761 aaacagcgct ccgatcagcc agagaagctc gtggcttctc atgatattaa cggcccatcc
     2821 actcccaaag ccacccagga tagctccgag tgaaccgccg gcgttgaaaa tgccgaacag
     2881 acgtttggcc tcccgtgaag tgaaaatcaa accggcaaac gtccaaaatt gagctacagc
     2941 aagaacacca acgattgcgg accagatgta gaaggcgaat atcgcccagg caaaattgaa
     3001 aaacacgata agaaaccaga acatgatcac attgctgatg atgaaggcgt tcgaggctag
     3061 aatgagccgg tagagcggaa ccctgaccgt ataacgcgtg taaacagcga tgatgactcc
     3121 cgcaagaacc gcggccaaga ggtaaacgta tgccagatag ctgatgggaa agtggctgag
     3181 aaagaaagag tcccttacaa ctttgatgac gttattggcg gcgagcagaa aaaaaagtag
     3241 cgagaccata atgccgacac gccgggcctc accgggtcgg atgtcaagaa agcgcgacaa
     3301 agtgtttggc agagaaaagt gattcattaa aattaactgt attgctattt tatagagacg
     3361 ttaatgcgcg gtcggcaggg ccatagaagt cggacattcc gatggatccg aggtcgatag
     3421 cggacacgtc gggaccgacg gtaccgagtt tcgatagatc attgtttcat ctcctcgttt
     3481 cgcgcattgc gcgcagttat gtcgacagac gcggaaaact cgaatcgccg cttttctttt
     3541 caagttagct cggcaatcac tcgatatcca gaggtcactt agagaacaat ctgacaagtc
     3601 agcgcgtcgg acgcatggac aacgatcgtg agatcgtcct tgaacgttat tggcggcgag
     3661 cagaaaaaaa tcgcttttcc acgctagatt ctctctggct gatcgaaatg tttagatgat
     3721 cgcccaagct gcgcgtttta agacgcggga ttctctgcgg ccgcttgctc acgatcgcgc
     3781 cgcgcctcat atgccttcag atgcctaaag gccagctgca gtagcgagcg atcagcgttt
     3841 aggacttggc tgccacggcg aagaaggtcg ccgagtatgt gatccgcttc gattcgagcg
     3901 tggcgttcca tgtcacgcag cattgatgcg gtgaggcgtg atcccggcgc tgttatagtg
     3961 ctgcgggtcg gttcgagaaa ttcggctcgt ggtgcaaagc cggcgctcgc agcaatcgat
     4021 cggcactcct ccagaagatc gagagccagg tgcttgcctc cagccgccgc aatgtctccg
     4081 atcgagctac gcatgaggca ggtgatcccc gccagcgtcg caaggaatat ccatttttcc
     4141 cacatctcga ggaggatgac ggagctcgcg cgtgattcga aattcgcctg gctcatttcg
     4201 tgcacgatag cgtcaatgcg cggcgagcgg ttttcatggc gctcgccgaa ggtcaatagg
     4261 tgcgaatcgt taagatgtac aatgcggccg atgtcatcga ccgtcgccgc gatcaaacat
     4321 tggccgccca agacgcgtgc cgcgccgaat cgttcgtcga gaaggtccag gtgccgcatg
     4381 ccgttcagga gcggcaggat tccggtctct ggtcctaccg ctgcggcaaa agaaccgata
     4441 gcgccattga ggtcataggc tttgcagctc aacaaaacca ggtcgaagtg ccggtctatt
     4501 ttatgagcct gaatggtcgg cgggactttg agcatgacat cgccaaagcg gcttttgatc
     4561 acgagaccgg tttcagcgag ctgagcggcc cgtttcggtc ggacgagaaa ggtaacgtcg
     4621 cggccggcct caagcaatct accgccaaag tatccgccga ccgcaccggc gccgacgacg
     4681 agaattctca taactgttcc ccttgtttgc tcgttgccac tccaatacaa gcatgtttgt
     4741 catcaaagct aaccgcgcgc ctttatagtt cgctctcgac caaaccacgc ccctgatatg
     4801 atcgccgtcg taagcacgac accgtagacg accaggccga aggctagtgc cgcgaacagc
     4861 cagttgagtg agccggttag gcgaagcgca agccagccac cgccgattgc aatgatgacg
     4921 cggaggaatc cagccaacag gggccataaa aggcgtgcgg cgccctgaga cgcgaaataa
     4981 agcgccatac ccaggccaaa aaatccatac gccggtccga cgatccgcag ataagcactg
     5041 cccgtctcga tcacacgcgg atcgggactg aagagcgcga gccacgcctg cggccagagc
     5101 gccgcgatca gcccgagcgt ctggctgatc gcaaaggcga cggcgccacc gaccaacgcg
     5161 atgcgtaccg ctctttgttt ttgaccagcg ccgatgttgg tccccacaag cgccaccagc
     5221 ggcgcgccca agccgaagat caccggtatc agtagatatt ccaaccgcac gccggtgccg
     5281 tagccggcga tagcgttagt tccagcggcg ctggcgacta gcgctgtggc gagcgccacg
     5341 gtcaagtttg tctgtaacga ggtgactgcg gcgacggcgc ccac
//
LOCUS       DASSWZ010000260         5925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_858982, whole genome shotgun sequence.
ACCESSION   DASSWZ010000260 DASSWZ010000000
VERSION     DASSWZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858982"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFN58_06260"
     CDS             complement(<1..518)
                     /locus_tag="VFN58_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET8917628.1"
                     /translation="MPELPEVETICRGLQPHLAGRRILNVKVRERRLRSLVDRRLAQS
                     LEGKTILRVGRIAKYILISLSGEVVWMIHLGMSGKLVHVDPETPEQKHDHILVRLDSG
                     HDLRYHDPRRFGLSLVATANKLRDLPQLKNLGLDPFDARFTGSYLHSFTKRSERRIRD
                     LLLDQQIVAGIG"
     gene            complement(532..1395)
                     /gene="rsmA"
                     /locus_tag="VFN58_06265"
     CDS             complement(532..1395)
                     /gene="rsmA"
                     /locus_tag="VFN58_06265"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET8917629.1"
                     /translation="MTTLYEEVRATLRESEFRPRKRLGQNFLVHENIIESILRLLDVS
                     PGDEIVEIGPGLGFLTRRLVDTARRVWAVEIDSALVNRLKQSFLGSHPAFHLIHNDIL
                     KVAPEEFLPPHKVKLVGNLPYSISTAVLFRLFDWREHFSSLVLMMQKEVGDRIASGPA
                     TKAYGTLSVWCQVHGRITERVSVSPEAFFPKPKVRSTILRIDLYQEPLIAVKELPLLR
                     GLVRAAFGQRRKTLGNALTSWLQRERNEIESVLRLEGIDPMRRGETLRVEEFLALTHA
                     LRASRLLTADG"
     gene            complement(1437..2435)
                     /gene="tsaD"
                     /locus_tag="VFN58_06270"
     CDS             complement(1437..2435)
                     /gene="tsaD"
                     /locus_tag="VFN58_06270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET8917630.1"
                     /translation="MLILGVETSCDDTAAAVLCDGRKLLANVVSSQDQVHSPYGGVVP
                     ELASRQHIQNILPIVDGALQKAGVTVQDLDGMAVTYGPGLVGSLLVGLSMIKGISYRW
                     HIPYVGVNHLEAHLLAIQLEHEVAFPYIALLASGGHTLLYWVRDIGAYVHLGGTRDDA
                     AGEAYDKVAKMMGLGYPGGRIIDNLAKGGDPKAVRFPRAQIKKGSYQFSFSGIKTSVW
                     HYLETQTKEEWRDQLADIAASFQEAVVDMLVKPTVKAAIACGVSRIVLAGGVAANSRL
                     REKMTESADAEGLTVYFPSPKFCTDNGAMIALAGYHALKRGRRDDFRLNADADLTL"
     gene            complement(2449..3009)
                     /locus_tag="VFN58_06275"
     CDS             complement(2449..3009)
                     /locus_tag="VFN58_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET8917631.1"
                     /translation="MPQEAKIREQDLKTRSPITPPAAKETTKPAVVTPEVPTGPLPDD
                     SSLLAKITPGTSPQRAASLRLTEEGRKLLEAGDASKALSRLEKTIVIDSTNPYGYFYL
                     AKTHYRLGRYKESLSFLEVAESRLSGEPFWLAEVHALRGENFRALGMVDKAEASYAQA
                     LRLNSGNRTANDALARLQGQSEATSR"
     gene            complement(3217..5529)
                     /locus_tag="VFN58_06280"
     CDS             complement(3217..5529)
                     /locus_tag="VFN58_06280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET8917632.1"
                     /translation="MKRPKLRILRIILVSLLIVTSSGLVFGGWYLRRLEETVAAKFEG
                     QKWRFPSKIYSDSHLLYVGINLRLQDLSEKLRRLGYHESQSPPKARGEYRLQPSRGLL
                     EIYLHDFSYPAEPFKGFPVRFSLQGTVITKIENSTTGEELFSLELEPELITGLYDRVW
                     EERRVVSLSEVPPLIVKAILAVEDERFYHHHGIDPTSVVRATWVNLRSGSVVQGGSTL
                     TQQLMKNFFLSDERKFSRKMKEAIMALIAERKYTKNEILENYLNEIYLGQRGSQGIFG
                     IWEASQFYFSKPLSELTVGEVALLAGLVRAPNRLSPYRSPDAATKRRNIVLVKLLDDK
                     IITRKQYEAAMRESLPRRELIKVTNDAPYYVDYLRRELAENYSNDVLTAEGFRIFTSM
                     DLQLQKMAEKSLAEGLKKLEAAYPALRRQGEEDNLEGAIVVLRPQTGEIKAMVGGRSY
                     QKSQFNRVFQAKRQPGSIFKPFVYLAALMAGGDGGKKFTPVTMVEDSPFKWSYDGQEW
                     EPGNYNDEYFGTVTFRRALEKSLNSATARIARDVGIKRVRDIAHRLGVQSSLPAVPSL
                     ALGAAEVTPLEVAVAFSTLANNGVRTRPLAVKQVVDSDGGAREKRDVRVEKVLTPQIA
                     FIMNHLLKGVFDRGTADAARRWGFTRPAAGKTGTTNDYKDAWFVGYTPDLLAVVWVGF
                     DNKAKLGLSGAQAALPIWTEFMKLATAGTPVTDFAPVPGIKIVEIDPLSGQLATPNCS
                     YVLREAFLERDEPTTTCPLHPGTPPASKFD"
     gene            complement(5648..>5925)
                     /locus_tag="VFN58_06285"
     CDS             complement(5648..>5925)
                     /locus_tag="VFN58_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET8917633.1"
                     /translation="GMTVQKLTPQIAESLGLEKADGVVVSAVDPGSVADEAGIRRGDV
                     ILEVDRKPIRSIDEYKKAVAGIRKGKGVLLLVRRGESTLFLALKPQN"
BASE COUNT         1364 a         1654 c         1543 g         1364 t
ORIGIN      
        1 ttgccaatcc ccgcgacgat ctgctggtcg agcaagagat cccggatgcg tcgctctgaa
       61 cgcttggtga acgagtgcag ataggagccg gtgaatcggg catcgaaagg atcgagccca
      121 agatttttca attgcggcaa atcgcgaagc ttgtttgccg tggccaccag cgataaacca
      181 aagcgccggg gatcatgata acggagatcg tgcccgctat cgagacgaac gaggatgtgg
      241 tcatgctttt gctccggtgt ctcgggatcg acatggacga gctttcccga catccccaga
      301 tgaatcatcc aaacaacctc gccgctgagc gaaatcagga tgtacttcgc tattcgtccg
      361 acccggagga tggttttgcc ttccaaagac tgagctaacc ttcggtccac aagggagcgt
      421 aagcgacgct cccttacttt aacgtttaga attcgtcgcc cggctagatg gggctgcagc
      481 ccgcggcaga tggtctcaac ttcaggcaac tcaggcatag gtttccgggg atcagccgtc
      541 ggccgtcagc aatcgggaag ctctcaaagc gtgagtcagg gcgagaaact cttcaactct
      601 tagcgtttct cctcgccgca taggatcgat tccttcgagg cgaagcacgc tttcgatttc
      661 gtttcgctct cgctgtaacc agctcgtaag cgcgttacct aaggtcttgc gccgctggcc
      721 gaaagcggcg cggacaagcc cgcgtagtaa aggaagctct tttacagcaa tcagcggttc
      781 ttggtaaaga tcgatcctta gaatcgtcga tctgactttt ggtttgggga agaatgcctc
      841 cggcgacacc gatactcttt cagtaattcg tccatggact tggcaccata ctgaaagagt
      901 cccgtacgct ttcgtggccg gaccgctggc gattctgtcg cccacttctt tctgcatcat
      961 caggaccaaa gacgaaaaat gctcccgcca atcgaagaga cgaaacaaaa ccgcggtgga
     1021 gatgctgtaa ggcaaattgc cgaccaattt gactttatgt ggcggtagaa actcctccgg
     1081 agccactttg agaatatcat tgtgaatcag gtggaaagcc ggatgggagc ccaggaagct
     1141 ttgtttcaaa cgattgacca aagcagaatc gatttcgacg gcccacaccc ttcttgcggt
     1201 gtccactaac cggcgggtca aaaaccccaa tcccggaccg atctcgacaa tctcatcccc
     1261 aggagaaaca tcgagaagcc gaaggatcga ctcgatgatg ttttcgtgaa caagaaagtt
     1321 ctgccccaac cgctttctgg gtcggaattc agattctcgg agcgtcgcgc gcacttcttc
     1381 atagagggtc gtcattttct atcaacccgg aaatcggcga attcttcgat ctatgcttat
     1441 aacgttagat cggcgtcggc gttgagccga aaatcgtctc ggcgtccgcg tttcaaggca
     1501 tgataacctg ccaaagcgat catcgcaccg ttgtccgtac aaaacttggg tgaaggaaag
     1561 taaaccgtca acccttccgc atccgcactc tccgtcatct tctcgcgcaa gcggctgttc
     1621 gcggcgacgc cgccggcaag cacaatccgg ctcacgccgc aggcgatggc ggctttaacg
     1681 gtcggcttga cgagcatgtc gacgacggcc tcttgaaagc ttgcggcgat atcggcgagc
     1741 tgatctcgcc actcttcctt ggtctgggtt tctagatagt gccacacgga agtcttgatg
     1801 ccgctaaaac tgaattgata ggaacctttt ttgatctgcg cgcgcgggaa gcggaccgcc
     1861 ttgggatcgc cccctttggc gaggttatcg atgattcgac ccccgggata tcccagcccc
     1921 atcatcttgg caaccttatc ataagcctca cccgccgcat cgtcacgggt gccgccgaga
     1981 tgcacatacg caccgatatc tctaacccag tagagaagtg tatggccgcc tgaagccagc
     2041 aacgcgatat acggaaatgc aacctcgtgt tcgagttgaa ttgctaacag atgggcctcc
     2101 aggtggttaa caccgacata agggatgtgc cagcggtagg agatgccttt aatcatcgac
     2161 agtcccacca gcagagaccc gaccagccct gggccgtagg tcaccgccat cccgtcaagg
     2221 tcttgcaccg tgacgccggc tttttgtaag gcgccatcga cgatggggag aatgttttgg
     2281 atgtgctggc gcgatgccaa ttccggaacc actccgccgt agggactgtg gacctgatct
     2341 tgagacgaaa cgacattcgc taaaagcttg cgtccatcgc acagcactgc agctgcggta
     2401 tcgtcacagg acgtttcgac acctaggatc aacatgcgtg taagatattc agcgggaagt
     2461 tgcctcgctc tgtccctgaa gccgcgctaa agcatcgttg gccgtacggt ttccggaatt
     2521 cagccgcagc gcctgtgcat agctcgcttc cgccttatcg accatcccga gagcacgaaa
     2581 attctccccg cgtagcgcgt gcacttcggc gagccaaaaa ggttctccgc ttagacggga
     2641 ttccgcaacc tcaagaaaac ttagagattc tttgtagcgc ccgagtcggt aatgggtttt
     2701 ggccaaataa aaatagccgt aggggttggt cgagtcaata acgatagtct tttccaagcg
     2761 ggacagagct ttcgaggcgt cgcccgcttc gaggagtttc ctaccctcct ccgtgagccg
     2821 aagcgacgca gcgcgttgcg gagaggtacc tggggtgatt ttagcgagca acgaactgtc
     2881 atccgggagc ggaccggtgg ggacctctgg cgtaaccacc gctggtttgg tcgtttcttt
     2941 agcggccggg ggcgtgattg ggcttcgggt ttttagatct tgttctctaa tttttgcttc
     3001 ctgaggcaag ggtgttctcg gcggttccgg taccggtgac ggagtagcag ctggcgatac
     3061 tttatcggat gaagctggcg gactagtgga cggttgagtt gtccggtaaa gtggtcgcgg
     3121 cgcacaacct tgcatagccc caatgatcca agcgcaggca aaaatgcgaa atggccattt
     3181 cggtaagcta tttgttttca tgaaaagaca attgcctcag tcgaactttg atgccggtgg
     3241 cgtcccagga tgcagcgggc aagtcgtcgt tggttcgtca cgttcgagaa aagcctcacg
     3301 caaaacatac gagcagtttg gtgtcgccag ctgaccgctc aacgggtcaa tttcaacaat
     3361 cttgatgcca ggcaccggag cgaaatcggt taccggggtc ccggccgtgg cgagcttcat
     3421 gaattctgtc cagatgggaa gcgcggcttg agcgccggaa aggcccagct tcgccttgtt
     3481 gtcgaaaccc acccaaacaa cagccagcaa atctggggta tacccaacga accaggcatc
     3541 tttgtagtcg tttgtggttc ccgtcttgcc ggcagccggt cttgtaaaac cccatcgtcg
     3601 tgccgcatcc gccgtgccgc ggtcgaagac tccttttaaa aggtggttca tgatgaaagc
     3661 tatttgcggt gttagcactt tctccactct gacatcgcgt ttttcccgcg ccccaccgtc
     3721 cgaatccaca acctgtttca ccgccagcgg acgggtgcgc acgccattat ttgccaaggt
     3781 tgaaaacgca acggcgactt ccagcggtgt cacctccgcg gcgccaaggg ccaatgacgg
     3841 caccgctggg agagaactct gaacaccaag acggtgcgcg atatcgcgca cccgtttaat
     3901 gcctacatcg cgggcgattc gcgccgtggc cgagttcaaa gacttctcaa gcgcgcggcg
     3961 aaaggtgacc gtgccaaaat actcgtcgtt atagttgccg ggctcccact cctgcccgtc
     4021 gtagctccac ttgaaaggag aatcctccac catagtcacc ggtgtaaact tcttgccacc
     4081 gtcaccaccc gccatgaggg ccgccaaata gacaaagggc ttgaagatcg agcctggctg
     4141 ccgtttcgcc tggaaaacac ggttgaattg cgacttttga tagcttcgac cgccaaccat
     4201 agccttgatt tcccctgtct gaggtcgaag cacaacgatt gcgccttcga gattatcttc
     4261 ttctccttgt cggcgtagag ccggataggc cgcctccagt tttttgaggc cctcggcgag
     4321 ggacttttcc gccatttttt gtagctgcag gtccatgctc gtaaaaatcc taaacccctc
     4381 agccgtgagc acatcgttgg agtaattttc cgccaattcg cgtcggagat agtcgacata
     4441 gtaaggggca tcattggtga cttttattaa ctcccgacgc ggtaagctct cccgcatcgc
     4501 cgcctcatat tgcttgcggg taataatctt gtcatccaac aactttacca gaacaatatt
     4561 tctacgcttg gttgctgcat ccggactccg ataaggagac agtctgttcg gcgctcgcac
     4621 cagacctgcc aacagagcta cctcgcccac cgtaagctcc gataacggct tggagaaata
     4681 gaactgcgac gcctcccaga tgccaaaaat cccttgagag cctctctggc caagatagat
     4741 ctcgttcaaa taattttcca agatttcgtt tttggtatat ttgcgttccg cgatgagcgc
     4801 catgatggct tccttcatct tgcggctgaa tttgcgctca tcgctgagga aaaaattctt
     4861 catcaattgc tgggtgagcg tactgccacc ttggaccacg cttccgctgc gaagattgac
     4921 ccacgtggca cgcacaaccg aagtcggatc gatgccgtgg tgatggtaaa agcgctcatc
     4981 ctcgacggcc aaaatggctt ttacgatcaa tgggggcact tcagatagag aaacaactct
     5041 tcgttcctcc cagacccgat catagaggcc cgtgatcagc tccggctcta gctcgaggga
     5101 aaacaattct tcccctgtcg tgctgttttc aatcttggtg atgactgtgc cctgcaatga
     5161 gaagcgaacc ggaaaaccct taaagggttc cgcaggataa gaaaaatcgt ggagataaat
     5221 ttctaagagt ccacgggatg gctgcaaacg atactcccct cgcgctttgg gcggagactg
     5281 actttcgtga taaccaagtc ggcgaagctt ctccgacaga tcctgcaagc gaagattgat
     5341 tccgacatag agtaaatggg agtcagaata aatcttcgac gggaagcgcc acttttgccc
     5401 ctcaaatttt gctgcgacgg tttcctcaag ccgtctgagg taccaaccgc caaaaacgag
     5461 cccgctggaa gtgacgatga gaagtgagac gaggatgata cgaagaattc gaagtttggg
     5521 ccttttcaaa cctaacactc caaatatgaa atttattgtt acctagttcc tacgcaaaag
     5581 caacaaaaag agggccgtgt gtgattaccc ggccctctca ctcgtagacg ggagataaga
     5641 gcaggaatta gttttgcggc ttgagtgcta agaaaagcgt gctctcgcca cggcgcacca
     5701 ataaaagaac ccctttccct ttacgaatcc cagcgactgc tttcttgtat tcgtcgatac
     5761 tgcgtatcgg cttcctatca acttcaagta tgacgtctcc tcttcggatg cctgcctcat
     5821 cggccacgct ccccggatct acggctgaca caacgacacc atccgccttt tccagtccca
     5881 aactttctgc aatctgcggc gttagcttct gcaccgtcat cccca
//
LOCUS       DASSWZ010000261        11414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1142031, whole genome shotgun sequence.
ACCESSION   DASSWZ010000261 DASSWZ010000000
VERSION     DASSWZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142031"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFN58_06290"
     CDS             complement(<1..450)
                     /locus_tag="VFN58_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917634.1"
                     /translation="MNSEEFLDEILVPVHEFCRFLDANPPAYCYVPLRTDEERIRVMK
                     SRLFNELRAADLFGTWLKTTAELDVKATMAESAHEEMVHAGLLSKRIRDLGAEPFDYK
                     PLPAQIAMFNAMEGLNETCERIAGLSLAGETVANYLIQMCLKSPDVPE"
     gene            complement(506..1219)
                     /locus_tag="VFN58_06295"
     CDS             complement(506..1219)
                     /locus_tag="VFN58_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917635.1"
                     /translation="MPQLRRLAISAEDPATLVAFYREVFGLQQIGEAGGAVFLSDGTF
                     NLALLPGEDGTATGLKGLGFETAQLESIRMKLARTANTDQSLVERDANTGIEYEMRDP
                     DGNLISLRTGAFDVSFRPGPVPIRHIALYTPDPQRLADFYCQVLDMKEVERTDRSSIF
                     VSDGYLNLALLYQRKEEPIGLNHFGFHVKSNEEMQTRAEQAGVRRGTARPDRIPFAEY
                     RVHDPEGNGIDISQKGWHV"
     gene            complement(1287..3803)
                     /locus_tag="VFN58_06300"
     CDS             complement(1287..3803)
                     /locus_tag="VFN58_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8917636.1"
                     /translation="MGKQPWRRQLLLAAMGLIAVLFVGGTLVASFQWISRPFPGFFLH
                     ENLTVGPYSLPNWSGGLAGVHALDRVVAVNGRSLRDRGELYDLVKSAPAGSRFDYRVI
                     RDSQTLEFAVPSMILSLHDWFLSFGIYVVLGIAFLIIGAAPCYFHATSPAALPLCFMV
                     MAVFVWFEATFDFMTAGVLPKEMRIFALTLTPSAALHLALLLKTGKPLRGSHPIYLAC
                     IYGIALFLGALNSLTFFGPFEAWVYIFRAGYVFTCVGALIFLGVIGSTLHGSLPDLER
                     SRLRVIFVGALLGLLIPTLSTVLTSSFQLAIPYNFALVPTVFFPLSVAYALLKYSLFD
                     LGNSLKVGLSRIALTAFLLAMYALVVFLLAPWVSTESDPFVPLFFSVLVVVVFNPLLR
                     WIEAVVDRYVYRQDYDPVRVQEEISLFLRSLAPPPALATGFVERVRDQMGIETVILAY
                     RPKETADEPLMVATDRVEQGSADVADGVRVLVASQESPYHHGISRGEVTTNPRFREER
                     AAMLALFDRWRTELLVPLVFERQVRGFVSFGAKRSRREYSAEDLRLLVFLTDQLALSL
                     ENGRLYEESVKAYAKAEATNKRLIEMDRVKKQFVANICHELRTPVSTIVGYAEVLLLN
                     PHLKGEGRDALDRLVNNGQELADLMDNLMNFSRIEGDTSVGQFEVVQLKEILLGLEMM
                     TQRLIRERPIQFGINMESTIDTIESDAQKLQQILVQLLTNALKFTERGKIDLTIQAIN
                     EGGKDFVKIAVADTGIGIKREDQEIIFEDFRQLDGSSTRQYGGTGLGLGLAKKLAVAL
                     GGTIQVSSEVGVGSVFSLLLPVKGSSISACKSGLAHVAQG"
     gene            complement(4001..4525)
                     /locus_tag="VFN58_06305"
     CDS             complement(4001..4525)
                     /locus_tag="VFN58_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HET8917637.1"
                     /translation="MHEESLHIEPGLRWYAIQTKANKEKEVEKRLSDLRIDVFLPWMR
                     TRRRIGSRFHWVLVPLFPGYLFCRLDLIESGKAARYAPGVRDFLKFGNHIAEVGSEIV
                     ASLHERCPEGIAQIELVSAKPGDPVKINEGPFSGLEAIFEKKMKGSERVTVLLDILGR
                     QTRLVLPSETIEKI"
     gene            5026..6960
                     /locus_tag="VFN58_06310"
     CDS             5026..6960
                     /locus_tag="VFN58_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HET8917638.1"
                     /translation="MDHNKEFETLIKRDRDLHLSRSWRGNLLGYLEKVKEDPTTAKLA
                     HARIYDRMMKAGVRDIHESGDPHVKRLYKDESIKVYDFFADEFFGIEKTISQIVRYFH
                     AASLKGEESRQVLYLMGPVGSGKSSLVEKLHRGLEESDSIYAIEGCPMFEEPLHLIPR
                     HLRKEFEKMLGVHVEGDLCPVCRFRLREEFSGKYEEFPVCTVEFSKRSRIGIGVVPPV
                     DPNNQDTSVLIGSEDISKLDLYSEGDPRVLELNGALNIGNRGIVEFIEVFKNEIEYLH
                     CMITATQEKVVPAPGRHGLVYVDTVIVAHSNEAEWQKFKSDHTNEAILDRIVVVRVPY
                     NLRLSEEVKIYQKIIRNSDFRAHVAPHTLEIASMFAILSRLEPTNKCDLMTKLKLYNG
                     EEVVEKGRTKKVDVQELRDASKREGMNGISTRFIMKALDNALSDNTAGNCINPINVRE
                     ALVNMVKEADLSDDARKQYLEFLQDTLHKEFLEILEKEITRAFVYSYQEQAEVLFQNY
                     LDHAEAYVNKSKVKDRNTKEELNPDEGFMKSIEEQIAIIGSAADGFRQEVIAYLWAAN
                     RRGEKIDYRSYEPLKEAIEKKLITSVRDLSRVITKARMRDEEQSEKYNTMVKNLLDRG
                     YCHSCVDMILKYAANNLWKD"
     gene            7006..8187
                     /gene="yhbH"
                     /locus_tag="VFN58_06315"
     CDS             7006..8187
                     /gene="yhbH"
                     /locus_tag="VFN58_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HET8917639.1"
                     /translation="METIFRPYHSASGQRSDRSAGDRLRHRQKVREAIRNNIADIIAE
                     ESIIGKDNNRIVKVPIRGVKEYRFVYGENVPGVGQGGDGELQPGQTVGKGKEGAQGHQ
                     AGDQAGVDYYETEVSLDELVEVMFEDLELPDLDRKNLRQMEVERQFRHKGFRKKGIRV
                     RLDKKRTARSRVKRKKASQRADVAALHGDRFPFHKDDLRYHHTVTETRRESNAVVLCI
                     MDTSGSMDTMKKYLARSFFFLLYRFLCTRYENVEIVFIAHHTEAQEVTEEEFFHKGES
                     GGTFISSGYNKALEIIYERYHPGLWNVYSFHCSDGDNFESDNPAALKAATELARISNL
                     FGYGEIKPLGAGYYGSSMIQFFSQIQEPNFQTVQIQRKEDIWPSFKTFLTKDRVRDDA
                     A"
     gene            8198..9631
                     /locus_tag="VFN58_06320"
     CDS             8198..9631
                     /locus_tag="VFN58_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET8917640.1"
                     /translation="MGAAYTIEDLQKWNDRITDLVQEFGLDPYPQEFEICDHEQMLSY
                     MVYSGMPSHYSHWSYGKAYEKLKTLYDYGLSGLPYEMVINSNPAIAYLMRDNTLALQV
                     LTMAHVYGHNDFFKNNFTFRTIRSEFTIETFKAHANRVRHYVEDPSIGLEKVERVLDA
                     AHALSLQCRRNLSIRKQTEDEQKDAKLRDSKPPDDPFQSVHRRKEWVSPDLAKIPVEP
                     EEDILLFIRDHNPQLAEWEKDLLTMVHEQAQYFIPQIETKIMNEGWASFWHKRILEAL
                     DLPQSLKIEFLIRHTQVLSPIPGGLNPYHVGMKVWEDIEKRWSEPDPEEEKEYGPKKK
                     NAQEKLFEVREVERDSSFLRRYLTEGLIRELNLFEYKSRGNEKVISRVADEDSWQGIK
                     DTLIQNVGTGTIPVIKVEDADYGNNRCLFLKHSHDGRDLQLEYAEKTLQYLRQLWARE
                     VALETIINDKKSLLGFADDKLTIKPVH"
     gene            9695..10810
                     /locus_tag="VFN58_06325"
     CDS             9695..10810
                     /locus_tag="VFN58_06325"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917641.1"
                     /translation="MTIHSDLTAIQNCIGNRLDDAAIGDRRIAGVIGDAPSHYSKSPA
                     LWNAAFDDLAMNAIYLPFDFDASHLRNFVSALRNSDRVMGINVTVPHKVQIMDFLDEL
                     DPSAERVQAVNTVVKNSAGRLIGHNTDGEGFVQSILGAQPGREAAFVKSLKGTDVLLL
                     GAGGAARAVAFHVSDQIESGKLVISNRTLNPAVSLAAELRKMGRNAIAIGEVEASDWA
                     PKVGLIINSTTKGQGGVRKLSHDEVTILEPFSSLAPAQAPVLRDFSGLEAEKQRRWSV
                     LAGTEVEDNNKVSMAIAASIPKAVGFYDLIYHPEETVFLRHGRLTGHPTMNGKAMIIN
                     QAVIAFCRHICSAELRARGMNNAETFRRILEVMYRAW"
     gene            10844..11251
                     /locus_tag="VFN58_06330"
     CDS             10844..11251
                     /locus_tag="VFN58_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917642.1"
                     /translation="MAKLTFILGLSGSGKTYLSERLKKETGAEVFTNLLADESGLTSL
                     IAFLRNGKDCIVDEVRFCLPAYREQILQSLSQITGLDMHWICYENDLETANWNVIHRT
                     NKGDPEGHLDINLRLHPHYTYPDDAEIVPIERI"
BASE COUNT         2924 a         3073 c         2942 g         2475 t
ORIGIN      
        1 ctccggcacg tcgggtgact tcagacacat ctgaatcaaa tagttggcca cggtttcgcc
       61 ggcgagagaa aggccggcaa tacgctcgca ggtttcattg agcccttcca tcgcgttgaa
      121 catggcgatc tgcgccggca acggtttgta gtcgaagggt tctgcgccca aatcgcggat
      181 gcgtttggac aaaagccccg catgcaccat ttcttcgtga gcggattccg ccatcgtcgc
      241 tttgacatct aactccgccg tcgtcttgag ccaggtaccg aacagatccg ctgcccgcag
      301 ttcattaaac aggcgactct tcatcacgcg aatgcgttct tcgtccgtgc gcaggggaac
      361 gtaacagtaa gccggcgggt tggcatcgag gaacctgcag aattcatgca cgggcaccaa
      421 gatttcgtct agaaattctt cgctgttcat atagttttgc ctgaacgatt tgaaccaatt
      481 tggagtttag actttcgtcg tggtcttaca catgccagcc cttttgcgaa atgtcgatgc
      541 cgttgccttc agggtcatgc acacggtact ccgcaaaagg aattcggtcc ggccgtgccg
      601 tgccgcgccg gacgccggct tgctccgccc tcgtttgcat ttcttcgttg ctcttgacgt
      661 gaaagccgaa gtgattgagc ccgatgggct cttcttttcg ctggtacaag agcgccaggt
      721 tcaaatagcc gtcggaaacg aaaatcgatg atcggtccgt tcgttcgact tctttcatgt
      781 cgaggacctg acaatagaaa tcggcgagtc gctgcgggtc gggcgtgtaa agagcgatat
      841 gacgaatcgg caccggtcct ggtcgaaacg atacatcaaa agcccccgta cgaagactga
      901 taaggttgcc atccgggtcg cgcatctcat actcgatgcc ggtattcgcg tcgcgttcga
      961 ccaggctttg gtcagtattc gcggtccgcg ctagtttcat gcgaatcgat tcgagctggg
     1021 cggtttcgaa acccaagcct tttagacccg tcgccgtgcc atcctcccct ggtagcagag
     1081 ctaggttgaa ggttccatcg gaaagaaaaa cagctccccc cgcctcgcca atttgttgaa
     1141 gcccaaaaac ttcccggtag aaggcaacca acgtggccgg atcttcggcc gagatcgcca
     1201 atcgacgtaa ttgaggcata gttcaattgt tcctttcccg tgagctggat tgaccttacc
     1261 acagcccggg acgcgacgaa atcagttcat ccctgagcca catgagctag tccgctttta
     1321 caggctgaaa tcgatgaacc tttcactggc agcagcaggc taaacacgct ccctactccg
     1381 acttcgctcg atacttgaat cgtaccgccc agcgctaccg cgagtttttt ggctagaccc
     1441 agacccagcc cagtgccacc atactgtcgg gttgaggagc cgtcgagctg acgaaaatct
     1501 tcaaaaatga tctcctgatc ctccctcttg atcccgatac ccgtgtcggc gacggctatc
     1561 ttcacgaaat cctttccccc ttcgttgatc gcctgaattg tcagatcgat ctttcctctt
     1621 tcggtgaact tgagcgcgtt ggttaaaagt tggaccagga tttgttggag cttttgcgcg
     1681 tcgctttcaa tggtatcgat agtcgattcc atgttgatgc caaactggat cggtcgttct
     1741 cgaatcagcc tttgagtcat catctctagc ccgagcaaga tttccttaag ttggaccacc
     1801 tcgaactggc cgactgaggt gtccccttcg atgcgcgaaa aattcatcag attgtccatt
     1861 aagtctgcca actcctggcc gttgttgaca agacgatcca aggcatcgcg cccctctccc
     1921 ttgagatgcg gattcaacaa caatacttcc gcgtagccga cgatcgtgct caccggtgtc
     1981 ctgagttcgt ggcatatatt ggcaacgaac tgttttttca cccgatccat ttcaatcaat
     2041 cttttattgg ttgcctccgc tttggcgtaa gctttcaccg actcttcata gagtcgtcca
     2101 ttttccagcg aaagagcgag ctggtctgtc aaaaaaacca acagacgaag atcttcggcg
     2161 ctatattctc tgcgcgatcg tttggcgccg aaggaaacaa atccccggac ctggcgttca
     2221 aagaccagcg ggaccaacag ctctgttctc catcgatcga aaagcgctag catagcggcg
     2281 cgttcttccc gaaacctggg gttcgtcgtc acctcaccac gggatattcc atgatgataa
     2341 ggactctctt gactcgctac taacactcgc acaccatcgg caacatctgc gctgccctgc
     2401 tccactctgt ccgtagcaac cattagcggc tcgtcggcgg tttcctttgg gcgataggca
     2461 aggatcaccg tctcaatgcc catttggtcc ctcactcttt cgacaaaacc cgtagccagt
     2521 gccggcggtg gggccaagga gcggagaaac aaactgattt cctcttgcac acgtacaggg
     2581 tcataatcct ggcgataaac atatcgatcc accactgctt caatccaacg aagcagggga
     2641 ttgaaaacga ccacgacgag gacggagaaa aacagcggga caaaagggtc actctccgtg
     2701 ctcacccacg gcgctaagag aaacaccacc aaggcgtaca tcgccaacaa aaatgcggtc
     2761 agcgcgatcc ggctcaatcc caccttcaaa gaattgccga gatcaaagag gctgtatttt
     2821 aagagcgcat aggcaacgga aaggggaaag aagaccgttg ggactaatgc aaagttgtag
     2881 gggatcgcta attgaaaaga gctggtaagc accgtactga gcgtgggaat tagaagacct
     2941 aacaaagctc cgacgaagat cacacgaaga cgggaccgtt ctagatctgg caatgaacca
     3001 tgcagcgtcg acccgatcac acccaagaaa atcaacgcgc cgacgcaggt aaacacgtat
     3061 cctgcgcgaa agatatagac ccaagcctcg aatggtccga aaaacgtcaa gctgttcaac
     3121 gcaccgagaa aaagagcgat cccatagatg caggcaagat aaataggatg agatcctcga
     3181 agaggtttgc cggtcttgag caacaacgct aagtgaaggg ctgcgcttgg agtgagggtc
     3241 aaagcaaaga tccgcatttc ttttggcaat actcccgccg tcataaaatc gaacgtcgcc
     3301 tcgaaccaga caaaaaccgc catgaccata aagcagagcg gaagcgccgc aggagatgtg
     3361 gcgtgaaagt agcagggcgc cgcaccgata attaaaaacg cgattcccag cactacataa
     3421 ataccaaagc tcagaaacca atcatgcaaa gatagaatca ttgaagggac ggcaaactcc
     3481 aatgtctgcg agtctcgaat gacgcggtaa tcgaaacggg agccggccgg cgcgctctta
     3541 acgagatcgt acaattcacc gcgatctcgt aacgaacgac cgttgacggc aacgacgcgg
     3601 tccaaggcgt gcactcctgc tagcccaccg ctccagttgg gaagagaata aggccccacg
     3661 gtcaggtttt cgtgcaagaa aaagccagga aaaggtctag aaatccactg gaacgaagcg
     3721 actagagtgc caccaacgaa tagcaccgcg atgagaccca tagccgccag cagcagttgt
     3781 cgtcgccacg gttgtttacc catgtaacct cctctgggca gattgcctgc tggatcggaa
     3841 aatcgccgca tgggcattta tcaaggcatg gcgagctgtg tcaagacggg gagcgatagc
     3901 agaatttacc ccaactaaaa gacaggtcag cgaaccggat agctggcaag gtcaagcacg
     3961 ggagaattcg cgcaaataga gcggacaaca gggatggagg ctagatcttt tcgatggttt
     4021 cgctcggcag gaccaaccgg gtttggcgac ccaaaatatc gagtaaaacc gtgacgcgct
     4081 cgctaccctt catttttttc tcgaagatcg cctcgaggcc ggaaaacggc ccctcattta
     4141 ttttgaccgg atcccccggt tttgccgata ccaattctat ttgggcgatg ccttccggac
     4201 agcgctcgtg caaggaggca acaatctcgc ttcccacctc agcgatgtga ttgccgaact
     4261 tgagaaaatc tctaacgccc ggagcgtagc gagccgcttt tccggattct atcagatcga
     4321 gccgacaaaa aagataaccg gggaaaagcg gaacgagaac ccagtgaaac cggctgccta
     4381 ttctccgccg cgttcgcatc cacggtaaga agacgtcgat acggagatcg gaaagccgtt
     4441 tttcaacttc cttctccttg ttcgccttcg tctggatcgc gtaccaacga agaccgggtt
     4501 cgatatgcaa agactcctcg tgcatgggac gagcttgctc ctgcaagatt tctcagagaa
     4561 tagatcttgg tgaggatata ttgtaacaaa caaaagcgct gatgataata tggccagaac
     4621 tgtttgccat caactgttat ttaactagga attttcttga ctttcctcgc aaataacggt
     4681 tcaatatgtg taagaaacgg gcgaactgaa taaaaagttc tttgatgtcg tctaaaggtc
     4741 aaagaaataa gttggctggc cgaccagaaa agcaacaaaa ggaggtcgaa cgcaagcctt
     4801 gaaatattcc cgattctccc ctgacggtat ccccaaagca aatccttttt acttctattt
     4861 tctatctcaa ggtgatgcgc gaaattaaaa tagtgctttc accggcgtat cttcatctcc
     4921 accgtcacaa tttattattg ttgcccgagc catcgacgtt tctgtcggtg gtaatttacg
     4981 tgtccgcgaa gactcaattc aatttagcaa agggaggctg ctggaatgga ccataacaag
     5041 gaattcgaaa cgcttatcaa aagggaccga gaccttcacc tttctagatc ttggaggggc
     5101 aatctcttgg gctatctcga aaaggtcaag gaagacccca ccaccgccaa attagctcac
     5161 gccaggattt acgacagaat gatgaaagcg ggcgtcagag acatccacga aagcggcgat
     5221 ccccacgtca agcggctgta caaagacgaa tccatcaaag tctacgattt ctttgccgac
     5281 gaattcttcg gcatcgaaaa aaccatctcc cagatcgttc gctattttca tgcggcgtcg
     5341 ctcaaaggcg aagaaagccg gcaggttctc tacttgatgg ggccggttgg ttctggaaaa
     5401 agctctttgg tcgagaaact gcacaggggc ttggaagaaa gcgattccat ctatgccatc
     5461 gaaggctgcc caatgtttga agagccgctg catctgatac cgcgccatct gagaaaagaa
     5521 tttgagaaga tgctgggcgt tcacgtggag ggagatctct gcccggtttg ccgttttcgg
     5581 ctgcgagaag aattcagcgg caagtatgag gagtttccgg tttgcacagt ggagttttcc
     5641 aagcgatcgc ggatcggtat cggcgttgtt ccgccagtcg atcccaacaa ccaggatacc
     5701 tcggtcctga tcggcagtga ggacatttcc aagctggacc tttactcgga aggggatccg
     5761 cgggtactgg aactgaatgg ggcattgaac atcggcaatc gcggtatcgt ggagttcatc
     5821 gaggtcttca agaacgagat cgagtatctg cactgcatga tcacggcgac ccaagaaaag
     5881 gtggtccctg ccccgggtcg gcatggtctc gtctacgttg acaccgtcat tgtcgcccat
     5941 tccaacgaag cggagtggca gaagttcaag tcagatcaca ccaacgaggc gatcctcgat
     6001 cgtatcgtcg tcgtccgagt tccgtacaac ttgcggctat cggaggaagt taagatttat
     6061 cagaagatca ttcgtaattc ggatttccga gcccacgtcg cgccccacac actggagatt
     6121 gcctcgatgt tcgcgatcct gtcgcgcctc gagccgacca acaagtgcga tctcatgacc
     6181 aagttaaagc tttacaacgg tgaagaagtc gtcgagaaag gacgaaccaa aaaagtcgac
     6241 gtccaagagc tgcgcgacgc ttccaagcgt gaaggcatga acggtatctc gacgcgcttc
     6301 attatgaagg ccttggacaa tgcgctctcg gataataccg ccggcaattg catcaatccg
     6361 ataaacgtac gcgaggcgtt ggtgaatatg gtcaaggaag ccgacctatc ggatgatgca
     6421 cggaagcagt acctggaatt tctccaggat accctgcaca aggagtttct cgaaatcctc
     6481 gaaaaggaga tcacgcgcgc gttcgtctat tcgtaccagg aacaggcgga ggttcttttc
     6541 cagaactatc tcgaccatgc tgaagcctat gtgaacaaga gcaaggtgaa ggatcgcaat
     6601 accaaagaag agcttaatcc ggacgaaggt ttcatgaaat ccatcgagga acaaatcgcc
     6661 atcatcggct cggcggccga cggctttcgt caagaagtca tcgcttacct ctgggcggcc
     6721 aaccgaaggg gcgagaaaat cgactaccgg agttacgagc cattaaaaga ggccatcgag
     6781 aaaaagctca tcacgtcagt tagagacttg agccgggtca tcactaaggc gaggatgcgt
     6841 gacgaggagc aatcggagaa atacaacacg atggtgaaga accttctcga ccgcggttac
     6901 tgccatagct gcgtcgatat gattctcaag tatgcggcga acaatctgtg gaaagactag
     6961 cattaccata gcggctttgt cgtcgtggta agtcacgtca ctgacatgga aacgattttc
     7021 cgcccctacc attcagcgtc cggccagcgc agcgaccgca gcgccggcga ccgtttgcgc
     7081 catcgccaaa aagtgcgtga ggcgatccgc aacaacatcg ccgacattat cgccgaggaa
     7141 tcgatcatcg gtaaagacaa caaccggatc gtcaaggtgc cgatacgagg ggtgaaggag
     7201 tatcgattcg tttacggcga gaatgtcccc ggagtcggcc agggcggcga cggcgaactc
     7261 cagcccggcc aaacggttgg caagggcaaa gaaggggcgc agggtcacca ggccggcgat
     7321 caagccggcg tcgattacta tgaaaccgag gtcagcttgg acgagctcgt ggaggtcatg
     7381 tttgaggatc tggagcttcc ggacctggac cgcaaaaacc tgcgtcaaat ggaagtggag
     7441 cggcagtttc gccacaaagg attcagaaaa aaaggcatcc gtgttcgttt agacaagaaa
     7501 cgaaccgcgc gctccagggt caaacggaag aaggcctccc agcgggcgga cgtggctgcc
     7561 ttgcacggcg accgctttcc ttttcacaag gatgacctca gataccatca taccgtgacg
     7621 gagacccgcc gcgagtcaaa cgcagtcgtg ctctgtatca tggacacttc cggttccatg
     7681 gacacgatga aaaaatattt ggcccggagc ttcttttttc tcctctatcg atttctttgt
     7741 acacgctatg aaaacgtcga aattgtcttc atcgcacacc acaccgaggc gcaagaggtg
     7801 accgaagaag aatttttcca caaaggcgaa tccggtggaa cgttcatctc atcgggctac
     7861 aacaaagcgt tggagatcat ctacgagcga taccacccag gcctgtggaa cgtttattcc
     7921 tttcactgtt ccgatggtga taatttcgag tccgacaacc ccgccgcact caaagcggct
     7981 acggagcttg cacggatctc aaatcttttt ggctacggcg agatcaaacc cttgggggcg
     8041 gggtactacg gcagcagcat gatccagttt ttttcgcaga tccaggaacc caacttccaa
     8101 accgtgcaga ttcagcgcaa agaggatatc tggccttcgt tcaaaacctt tctcactaag
     8161 gaccgcgtcc gcgacgacgc ggcataaatc cacgtctatg ggcgcagcct acaccatcga
     8221 agatcttcag aaatggaacg accggattac ggatctggtc caagaattcg gccttgatcc
     8281 atatcctcag gaattcgaga tttgcgatca cgaacagatg ctctcgtata tggtctactc
     8341 cggtatgcct tcccattact cccactggtc ctacggcaag gcctacgaga agctgaaaac
     8401 gctgtatgac tacggcttga gcgggctccc ttacgagatg gtgatcaact cgaatcccgc
     8461 catcgcctat ctcatgcgtg acaataccct tgcgctccag gttctcacca tggcgcatgt
     8521 ctacgggcac aacgattttt tcaaaaacaa ctttactttc agaaccatcc ggtccgaatt
     8581 cactatcgaa acgttcaaag cacacgcaaa ccgcgtgcgt cattatgtcg aggatccgag
     8641 catcggactc gagaaagtgg agcgcgttct ggacgccgcc catgcccttt ccctccagtg
     8701 ccggcgcaat ctttccatcc ggaaacaaac cgaagatgag caaaaagacg ccaagctgcg
     8761 ggattcgaaa ccgccggacg acccgtttca atcggtccac cgacgcaagg agtgggtgtc
     8821 acccgatttg gcgaagatcc ccgttgagcc ggaagaagat attcttcttt tcattcgaga
     8881 ccacaatccg cagctagcgg aatgggaaaa agacttgctg acgatggtcc acgaacaagc
     8941 ccagtatttt attccgcaga ttgagaccaa gatcatgaac gaaggctggg cgagcttttg
     9001 gcacaaacgc atccttgagg ctttggatct tccgcagagc cttaaaattg aatttctcat
     9061 tcggcatacg caggttttga gtcccatccc cggcgggctc aacccctatc acgtcggcat
     9121 gaaggtttgg gaagacatcg aaaagcgttg gagcgagccg gatcctgagg aagagaaaga
     9181 gtacggaccg aagaaaaaaa acgcccagga aaaactcttt gaagtgcgcg aagtcgaacg
     9241 ggacagctct tttctacggc gctatctgac cgaaggcctc atccgcgaac tcaatctctt
     9301 cgagtacaag tcccgcggca atgagaaggt catcagccgg gtggccgacg aagacagctg
     9361 gcagggaatc aaagacacct tgattcaaaa cgtcggtacg ggaacaatcc cggtcatcaa
     9421 ggtagaagac gccgattacg gcaataaccg ttgcctcttt cttaaacaca gtcatgacgg
     9481 gcgtgacctg cagctcgaat acgccgaaaa gaccctccaa tatctgcggc agttatgggc
     9541 gcgggaggtt gctctcgaaa ccatcatcaa cgacaagaaa tccctccttg gctttgctga
     9601 tgataaactg acgatcaaac cggttcatta gcaattcttt ttcaggccaa acctttagtt
     9661 ggtatgtaac cctcaaacgt cgcggagaaa cgcgatgacg atccacagcg atctaacggc
     9721 tattcagaac tgcataggga accggctcga cgacgccgcc atcggcgacc gccgaatagc
     9781 cggtgttatc ggcgatgcgc cgtcgcacta ttcgaagagt cctgcgttat ggaacgctgc
     9841 cttcgatgat ttggctatga atgcaatcta cctgcccttt gacttcgacg cgtctcacct
     9901 ccgaaatttc gtctccgctt tgcgaaacag tgatcgcgtc atgggaatca acgtgaccgt
     9961 tccccacaaa gttcaaatta tggatttctt ggacgagctg gatccgagcg cggagcgagt
    10021 ccaggccgtc aacacggtgg ttaaaaactc cgcgggccgg ctgatcggcc acaacaccga
    10081 cggcgaaggc tttgtccaaa gtatcctcgg ggcacaaccg ggacgggaag cagcctttgt
    10141 caaatcgctt aaaggaaccg acgtgttgct tttgggcgcc ggcggcgcag cgcgtgcggt
    10201 tgcgtttcac gtctccgatc aaatcgagag cgggaagctt gtgattagca accgaacctt
    10261 gaaccctgcg gtctccctcg cagccgagct gcggaagatg ggccgcaacg cgatagcgat
    10321 tggcgaggtg gaagcctcgg actgggcacc gaaagttggg ctgattatca acagcactac
    10381 gaaaggtcaa ggaggtgtgc gtaagttgtc ccacgacgag gtgaccattc tggagccctt
    10441 ctcgtcgctc gccccagccc aggccccggt gttacgggac ttctccggcc tcgaggcgga
    10501 aaagcaacgg cgctggagcg tacttgccgg caccgaggtt gaagacaaca ataaagtttc
    10561 gatggcgatt gccgcttcaa ttcccaaagc tgtaggtttc tacgatctga tttaccatcc
    10621 cgaagagacc gtctttctgc gccacggccg gctaaccggc catccgacaa tgaacggcaa
    10681 agccatgatc atcaaccaag ccgtcatcgc cttttgcagg cacatctgca gtgcggagct
    10741 ccgggccagg gggatgaata acgccgaaac attcagacgc atcttagagg tcatgtatcg
    10801 cgcgtggtag ctggttcgcc gtagcgccac cgaggagtaa ctaatggcaa aacttacctt
    10861 tatacttggc ttgtccggtt ccggaaaaac ttacctatcg gagcgattga agaaagaaac
    10921 gggcgcagaa gtatttacca atttgctggc agacgagagc ggactcacct cgctcattgc
    10981 attcttgcgc aacggcaagg attgtatagt tgacgaggtg agattttgcc tgccggctta
    11041 tcgggagcaa atcctgcagt ctctttcaca aatcaccggc ctcgatatgc actggatttg
    11101 ctacgagaac gatctggaga cagccaactg gaacgtcatt cacagaacaa ataaaggcga
    11161 cccggaaggg catttggata taaacctccg gctgcaccct cactatacgt accccgacga
    11221 tgctgaaata gttccgatcg agcgcatctg atcacgctcg ttttactctg tccgtcgtct
    11281 tctctttacg atccggtggg atagccggtt gaatgcccga atcggcgagt ttcaacgctt
    11341 tcaccaatgt ggatttgtag caagtcctct ccaacaacta acggcccgga gtatttttct
    11401 ctggttgccg ggat
//
LOCUS       DASSWZ010000262         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1043194, whole genome shotgun sequence.
ACCESSION   DASSWZ010000262 DASSWZ010000000
VERSION     DASSWZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043194"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFN58_06335"
     CDS             complement(<1..896)
                     /locus_tag="VFN58_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET8917643.1"
                     /translation="MREIAYYEAKFEALHEIMTADSTVHLMNGTFLSLSPHRHVYQEL
                     AKKYGDRIMSPPISELGFCGLATGAAMAGLRPIVDISTGSFIFEGWPQVANEAANAFS
                     MSGGQTRVPVVFHLFHGLRGGGAAQHSASPQAMLWNNPGLEIVLPSSPRDVKGLLKSA
                     VASDNPTIFVDHVQLLDLKGDVPENDAPIPFGVADIKRKGSDVTVIATSYMVQRCLQV
                     AAKLASERISMEVVDPRTLVPFDMATLMVSVEKTGRVVIVDETHQSCGVAAEIAARIA
                     ESGFDKLKTRIRRVSTLDVPVP"
     gene            complement(906..1925)
                     /locus_tag="VFN58_06340"
     CDS             complement(906..1925)
                     /locus_tag="VFN58_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8917644.1"
                     /translation="MLPKVPRETLLDLCRRMLVIRHFEESLVPLHDKGVFGGHYHLYI
                     GQEATGVPALSCLRADDYLFTTHRNHGHLLARGADPKRLCAEILGRVDGYNKGKGGSF
                     HTVVPSLGFLHTSAVVAGIIPLATGTAYAAKALKNGRITVCLFGDGALEEGAAPEAFN
                     LASLWKLPVLFLCENNGKYGAGRATGAAQSSTMAAFPLTDLPRAYKVPAQQIDGLDSG
                     MVHATISEAVEKIRRGEGPQFIETQTVRWPGSETNWPSVTQPTDSSLAWDVSGVPEKV
                     REWYRSCDPLLIYIRELVDAKQATKEEIAAIEKKVKAEIADAVEFAVASPYPRPEEAA
                     QQYFA"
     gene            2045..3046
                     /locus_tag="VFN58_06345"
     CDS             2045..3046
                     /locus_tag="VFN58_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917645.1"
                     /translation="MTSRRLLAPLLLFVGFFFSIPPVFSQARNLKKIHVGVPAVSMGN
                     IIIFFSKEAKIYDKYGLDAEPVVMSGSGIASRALIAGSVTISPITTPAVMSAVLAGSD
                     MVILAHTMPGVIQSLMVRPDIKRAEDLKGKTIGVTTFGSLGDFLVRYLAKQKGLNPDK
                     DFALRQIGADPERLISLRQGSIQAATLSHPSFILAQRAGFQVLWDFFKDVEYPWSEIA
                     TTRTLIKQDRDLVMRYMRAHLEGIARFKQEPELAKKVIKKMLRLDDESLAQESWELFA
                     KHRLATPYPNIKGMKTSYEYVAQTRPDVYKHKPEEFADASFVEELDKSGFIKKLYEK"
     gene            3098..3535
                     /locus_tag="VFN58_06350"
     CDS             3098..3535
                     /locus_tag="VFN58_06350"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET8917646.1"
                     /translation="MNQDDYIKDRVDGQIEWYDRKSGTNQRWFRWLRIVEIIAAASIP
                     LLVGYADSITELKVVIGILGLLIAVIAGVLGLYQFQENWTGYRTTCEALKQERYLFLT
                     KTQPYDQGDSFSLFVQRVENLLSKEHANWAQHIGAGGKPKDHP"
     gene            complement(3547..>4063)
                     /locus_tag="VFN58_06355"
     CDS             complement(3547..>4063)
                     /locus_tag="VFN58_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HET8917647.1"
                     /translation="GDGKRLGIYGFGAAAHIVAQVARYQNREIYAFTRPGDEEAKKFA
                     LALGAFWAGGSNELPPVKLDAAIIFAPVGDLVPQALRAVGKGGVVVCGGIHMSDIPSF
                     SYEILWEERSLCSVANLTRQDGEKFFALAPRVPVRTEVQTFPLEEANEALARLRSGKI
                     QGAAVLTVDGI"
BASE COUNT          935 a         1109 c         1106 g          913 t
ORIGIN      
        1 tagggtactg gcacgtccag cgtcgagacg cggcggatac gcgtcttgag tttgtcgaag
       61 ccggattcgg cgatgcgcgc ggcgatctcc gcggcgacgc cgcaactctg atgagtttca
      121 tcgacgatga cgacgcgacc ggtcttttct acggatacca tcagggtcgc catatcgaag
      181 ggtacaagtg tccgcgggtc gacgacttcc atgctgattc tctcgcttgc gagcttcgcg
      241 gcaacctgca agcagcgctg gaccatatac gaggtagcga taaccgtcac atcactccct
      301 ttgcgtttta tatcagcaac gccaaaagga atcggcgcgt cgttttccgg cacgtcgcct
      361 ttcaggtcga gcagttgaac gtgatcgacg aaaatcgtcg gattgtcgct cgccaccgcg
      421 ctcttaagca gacctttcac atctctcggc gatgaaggaa gaacaatttc caatcccggg
      481 ttgttccaga gcatggcctg ggggctggcg gagtgttgcg ctgcgccacc gccacgaaga
      541 ccatgaaaaa gatggaatac gactggcacg cgggtctgcc cgcctgacat ggagaaagca
      601 ttagcggctt cattggcaac ctgcggccag ccttcgaaga tgaaactccc ggtgctgata
      661 tcgacaatcg gcctgaggcc ggccatcgcg gccccagttg cgaggccaca aaaccccagc
      721 tcggatattg gcggcgacat gatccggtcc ccgtatttct tggccaactc ttggtagacg
      781 tggcggtgcg gactcagact caagaaggtg ccgttcatga gatgcactgt cgaatccgct
      841 gtcatgattt catgtagcgc ctcaaacttt gcttcgtaat aggcaatttc tctcatgact
      901 ggctctcaag cgaaatattg ttgggccgcc tcttccggcc ttggatacgg actcgctacg
      961 gcgaattcca ccgcatcagc gatctctgct ttcactttct tttcgatggc agcgatttct
     1021 tccttggttg cctgcttggc atcgacgagc tcgcgaatgt atatcagcag cggatcgcag
     1081 ctccggtacc actcacgaac tttctccggt acgccactta cgtcccatgc aagcgacgaa
     1141 tctgtcggct gggtgactga tggccaattg gtttcgctgc cgggccaacg tacggtttgc
     1201 gtttcgataa actgtggtcc ttcgccacgg cgaatctttt ctacggcttc tgaaatcgtg
     1261 gcatgtacca tccctgaatc gagaccgtcg atctgctggg caggaacttt gtatgccctt
     1321 ggcaaatcgg tcagcgggaa cgccgccatc gtcgatgact gcgccgcgcc ggtggcgcgc
     1381 ccggcgccgt atttgccgtt gttctcacaa agaaagagca ccggcagttt ccaaagcgaa
     1441 gcgagattaa acgcttcggg cgccgcgcct tcttccagcg caccgtcgcc aaaaaggcag
     1501 acggtgatgc ggccgttctt taaggctttg gcggcgtatg ctgtgccggt cgccagggga
     1561 attattcctg caacgacagc ggaagtgtgc aggaaaccaa gggagggaac taccgtgtgg
     1621 aacgatcccc ccttgccctt gttatagcca tcgacacgtc ccaaaatttc ggcgcagagt
     1681 ctcttcgggt cggcaccacg ggccaagagg tggccgtggt tcctatgcgt tgtaaacagg
     1741 taatcgtcgg ctctcagaca ggacaacgcc ggaactcccg tggcctcctg gccgatgtaa
     1801 aggtgataat gtccgccgaa cacacctttg tcatggagcg gaacgaggct ttcttcaaaa
     1861 tgccgtatga caagcatccg gcggcataaa tcgagcaagg tttcgcgcgg tactttgggc
     1921 agcatgataa cctgtgactt aattcaaaaa cctacaattt gtcaacgtcg cgggtttgat
     1981 tgacattgtt tcgacgagag gactatatgc caaagaagac gatctcgatc aggagggccc
     2041 gtcaatgaca agtcgtcggt tgttagcgcc tctgctgttg ttcgtgggtt ttttcttttc
     2101 aatacctccg gtcttttccc aggcgagaaa cttaaaaaaa atccatgtcg gggtccctgc
     2161 cgtcagcatg ggcaacatca ttattttctt ttccaaagaa gcgaagatct acgacaagta
     2221 tggtctcgac gccgaaccag tagtgatgtc gggctccggt atcgcctcca gggctctgat
     2281 cgccggcagc gtgaccatat cgcctatcac taccccagct gtgatgagtg ccgtgcttgc
     2341 aggatcggat atggtgattt tggcccatac gatgccgggc gtgattcaat cattgatggt
     2401 tcgcccggat ataaaacgag ccgaggatct taagggaaaa acaatcggtg tcacaacctt
     2461 cggttcgctg ggtgacttcc ttgtacgata ccttgccaag caaaaggggc ttaatccgga
     2521 caaggacttt gcgctacgtc aaatcggtgc cgaccccgag cgcttgatct ctctgcggca
     2581 gggaagtatc caggcggcaa cgttgtcaca tccatcattt atccttgcgc agcgggccgg
     2641 atttcaggtg ttatgggatt ttttcaaaga tgtggagtat ccctggagcg agatcgcaac
     2701 cactcgcact ctgattaagc aggaccgtga tttagtcatg cgttatatgc gggcgcatct
     2761 cgaaggcatc gcccgtttta agcaggaacc tgagctagcc aagaaggtga tcaagaaaat
     2821 gctgcgtttg gacgatgagt cgctggcgca agaatcttgg gagctatttg ccaagcatcg
     2881 cctggccact ccgtatccta acatcaaagg aatgaaaacc agttacgaat atgtcgccca
     2941 gacgcgcccg gacgtttaca agcacaagcc ggaagagttc gccgacgcga gctttgtcga
     3001 ggagctggac aaaagcgggt tcattaagaa gctgtatgag aagtgaagaa gtcaaatcaa
     3061 gcttgcgcct ttgtctgaca agacggagta ccatgccgtg aatcaagacg attacatcaa
     3121 ggatcgcgtg gacggccaga tcgagtggta tgatcgcaag agcggcacga atcaacgatg
     3181 gttcaggtgg ctgcggattg ttgagattat cgcggccgcg tcgattccat tgctcgttgg
     3241 ctacgcagat tccatcactg agctcaaagt agtgatcggc atccttggcc tcttgatcgc
     3301 agtaattgcc ggcgtactag ggctttatca attccaagaa aactggaccg gctaccgaac
     3361 gacatgtgag gccttaaagc aggagagata tctctttcta acgaaaaccc aaccctacga
     3421 tcagggtgat tcgttttctc tcttcgtcca acgcgtcgaa aacctcctct ccaaagaaca
     3481 tgcaaactgg gcgcaacaca tcggcgccgg cgggaagcca aaggatcatc cttaatcaat
     3541 tggtgtctaa atcccatcca ctgtcagcac cgctgcaccc tgaatcttgc ccgagcgcag
     3601 acgagccaaa gcctcattag cctcttccaa gggaaaagtt tgaacttcag tgcgcacggg
     3661 aacgcgcggc gcgagagcga agaatttctc gccatcttgc cgcgtgagat tggcgaccga
     3721 gcagagggaa cgttcctccc ataagatttc gtaagaaaag gaagggatgt cactcatatg
     3781 gatgccgccg cacacgacaa cgccgccctt ccctacagct ctgagagctt gtggaacgag
     3841 gtcgccgacc ggcgcgaaaa taatggcggc atccaatttc accggcggta attcgttgga
     3901 acccccagcc caaaaagcac cgagggcgag agcgaatttc ttcgcttctt catcgcccgg
     3961 gcgcgtgaaa gcatagatct cacggttctg gtagcgcgca acctgagcga cgatatgcgc
     4021 cgcggcgcca aagccgtaga ttcccagacg tttaccatcg ccc
//
LOCUS       DASSWZ010000263         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_746065, whole genome shotgun sequence.
ACCESSION   DASSWZ010000263 DASSWZ010000000
VERSION     DASSWZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746065"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFN58_06360"
     CDS             <1..474
                     /locus_tag="VFN58_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917648.1"
                     /translation="RTGRIAGFTSPPGTVELTKGMPHRILISMGDLPNRYPFPYVCVT
                     TTKSYLVSNRSTVKRIMMALIEGTHFFKTQKEESKKIFARYSRQNNEGYLEAGYQANA
                     KLFERVPFVTKDGMEIQIKEALARKPGATLKVNDIVDDSIVVELEKEGFIDRIYK"
     gene            583..1698
                     /locus_tag="VFN58_06365"
     CDS             583..1698
                     /locus_tag="VFN58_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917649.1"
                     /translation="MSDQAKILIVDDSADIVEFLADLLQATGYAICTASSGPEALEQI
                     ECERPDLVLLDVMMPNMTGHEVCRKIRENPATVFLPVVMLTAGDANVERIKGIEAGAD
                     DFLSKPPSHPELLARVRSLIRIKRLHDTVQAQMNQISEWNKTLETRVQEQVAQLERLE
                     RLKRFFSPHLAELIVTGGAEDPLKSHRRQISVVFIDLRGFTAFAETNEPEEVMDVLWE
                     YHAELGPVILKYEGTLERFTGDGMMVFFNDPVLVPDPEERAVRMAVSMRDRVATLQRK
                     WANRGYTLDLGAGITAGYATIGAIGFEGRKDYAAIGTVTNLAARLCDQALPGQILISR
                     RFYSAVQSLVEVESVGELTFKGFQRPLQTYNIVRLRV"
     gene            1890..2030
                     /locus_tag="VFN58_06370"
     CDS             1890..2030
                     /locus_tag="VFN58_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917650.1"
                     /translation="MFLNKTAGLFFCTILQRKGTTMVAIYMATELSKEKKDALIEYLK
                     AL"
     gene            2047..>3393
                     /locus_tag="VFN58_06375"
     CDS             2047..>3393
                     /locus_tag="VFN58_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8917651.1"
                     /translation="MAKPMITLLSKEHPITEKGMMEFQPFGVEPDGTKIQDLSGVAMR
                     AMLEYLEEYASRSQGPEAGRRAVEELVQRLNERIPDRAFHVTSKFLRTPWNGYSNEFS
                     AFVGQWCLDISGDPRFHLNYAREKAISPIIQTLGRPFSVPMIYKMSAYFSQRYSKDSF
                     FTEAVSISERSAILRMIFKERALRHFGPYLKACARLWCDGHKGYLTGVPEMFHDLPPA
                     EVRDVHCIAEGDECCEWEVTWSGKERKVWPVVTSLARRVLQPEIAQREKIIDEQLRSL
                     NSWDQELQDAYIQQQQLAAELQRRVDQLTTLHETGLLFTSILDREKLIEIVLGTVVQK
                     LNYDRVMFAFFDSDRKVMYDARLMGVHEEVQSFARSHEIPVTDPETIEGTVLLRGEPV
                     LIGDIRQVVDRVHPLNRQLVEMAKAKSFVSVPLKVKNEVLGSLTVDRVQQYALTDDD"
BASE COUNT          803 a          896 c          969 g          725 t
ORIGIN      
        1 cgcaccggca gaattgcggg ctttacttca ccacccggta cagtcgagct caccaagggt
       61 atgccccacc gcattcttat cagcatggga gacctgccga accgctatcc gtttccctac
      121 gtctgcgtga cgacgacgaa gagctatttg gtgagcaacc gctctacggt caaaaggatc
      181 atgatggccc tcatcgaggg gacgcacttt ttcaaaaccc aaaaggagga gagcaagaag
      241 atctttgcca gatactcgcg ccaaaacaac gagggttacc tcgaagcggg ctatcaggcc
      301 aacgccaaac tttttgagcg cgtgccgttt gtgactaagg atggaatgga gattcagatc
      361 aaggaggcgc tggcgcgcaa acccggcgca accctcaagg tcaatgacat tgtcgacgat
      421 agcatcgtgg tggaactgga gaaggaaggg ttcatcgatc gaatctacaa atagtggtct
      481 tacttccgga aattctccac catggacatg aatgttttct tgcacggctt tccgggccgg
      541 ctgctgcgtc gcgcgtcata gcatgctgtc cctgggggag ccatgtccga tcaagctaaa
      601 attctcattg ttgatgattc cgccgacatc gtggaattcc tggctgatct cctgcaagcc
      661 acgggttacg cgatctgcac cgctagctcc ggcccggagg ccctcgaaca aatcgagtgc
      721 gagcgacccg acctcgtact gcttgatgtg atgatgccga acatgacggg ccatgaagtg
      781 tgtcgaaaga tccgagagaa ccccgcaacc gtgttcttgc cggtggtgat gctcaccgcg
      841 ggagatgcaa atgtggaacg gatcaaaggc atcgaagccg gggccgatga tttcctgagc
      901 aagccgccga gccatccaga actgctggca cgcgttcgct ccctgatccg aatcaaacgg
      961 ctccatgaca cggtccaagc ccagatgaac caaatttcag aatggaacaa aaccctggag
     1021 acgcgcgtgc aagaacaggt cgcgcagctc gagcggctag agcggctcaa gcgctttttt
     1081 tccccacacc tcgcggaact catcgtgacg ggcggcgccg aagatcctct taagagccac
     1141 cgccgtcaaa tcagtgtcgt attcatcgat cttcgagggt tcaccgcttt tgcggaaacc
     1201 aacgagccgg aagaagtcat ggacgtactc tgggaatacc atgctgagtt gggtccggtg
     1261 attctgaagt acgaagggac cctggaacga tttaccggtg acggtatgat ggtgtttttt
     1321 aacgatccgg tacttgtgcc ggatccagaa gagcgggcgg ttcgtatggc ggtgtcgatg
     1381 cgcgaccgcg tcgcaacgct ccagcgtaaa tgggccaacc gaggctatac gttggatctg
     1441 ggagctggaa tcaccgctgg ctatgcaacc atcggagcca tcggtttcga aggccggaag
     1501 gactacgcag ccatcggcac ggtcacgaac ttggctgccc ggctttgtga tcaggccctc
     1561 ccgggccaga tcttgatatc gcggaggttt tacagtgccg tccagtccct cgtcgaagtg
     1621 gaatcggtgg gagaactaac cttcaaagga ttccaacggc ccctgcagac ctacaacatc
     1681 gtccggctta gagtgtagtg agcggcggtt ttcgtaaggg aggaggactt agggtcaagc
     1741 ctatatcgag acgcggttag cggacaatca ttcagtcttg ccaccggtgc gccgtgcagt
     1801 agacgagagg gggaactgat cgcatcggcc caaagagttc tatcgtggga acgatgtcta
     1861 cgactgggac aatgtgggat tcaagtggga tgttcctgaa caaaacggcc ggactttttt
     1921 tctgtacgat tctgcaaaga aagggaacga caatggtggc catctatatg gcaaccgaac
     1981 tatcgaagga aaagaaagac gccctcattg aatacctaaa ggcgctgtaa aacaaagttg
     2041 agccacatgg caaaacccat gatcacacta ctttccaaag aacatcccat caccgaaaaa
     2101 ggcatgatgg agtttcagcc gttcggcgtg gaacccgacg gcacgaaaat tcaagacctg
     2161 agcggggtgg ccatgcgtgc aatgttggaa tatctagaag aatacgccag ccgatcccaa
     2221 ggtcccgagg cagggcggcg cgccgtcgag gagttggttc agcgtctaaa tgaacggatt
     2281 cccgatcgag cttttcatgt gacttctaag tttctccgga ctccgtggaa tggttattcc
     2341 aatgagttct cggcgttcgt cggccaatgg tgcttggata tttctggcga tccgcggttt
     2401 catctcaact atgcgaggga aaaagcaatc tcgccgatca ttcaaacatt gggccggcct
     2461 ttctcagttc cgatgatcta taagatgtcc gcctactttt ctcagcgata ctccaaggat
     2521 tcgtttttca cggaggcggt gagtatctcc gagcgctcgg cgatccttcg catgatcttc
     2581 aaggaacggg ccttgcgcca ttttggcccc tatctcaagg catgcgccag gctctggtgt
     2641 gatgggcaca agggctattt gacgggagtc ccggaaatgt ttcacgatct ccctcctgcc
     2701 gaagtcaggg acgtccattg catcgccgag ggagatgagt gctgcgagtg ggaggtcaca
     2761 tggtcgggaa aagagcgcaa ggtctggcct gtggttacat cgttggctcg ccgggttctt
     2821 cagcccgaga tcgcgcaacg cgagaaaatc atcgacgagc agttgcgttc actgaactcc
     2881 tgggatcagg aactgcagga cgcctacatt caacaacagc aactggctgc cgagctacag
     2941 cgccgcgttg atcagttgac cacgctccat gaaaccggcc tgctgttcac ctcgatcctt
     3001 gaccgtgaga aactgataga gattgtgctg ggaaccgtcg ttcaaaagct aaactacgac
     3061 cgggtcatgt tcgccttttt cgatagcgac cgaaaagtga tgtacgatgc tcggctcatg
     3121 ggcgtgcacg aggaagtcca gagtttcgcc cgttcgcacg agattccggt caccgatcca
     3181 gagactatcg agggaacggt tctgctgcga ggggagccgg tgctgattgg cgacatccgg
     3241 caagtggtgg atcgcgtgca tccgctcaac cggcagctcg tcgagatggc gaaagcgaag
     3301 tcgtttgtgt cggttcctct gaaagtcaaa aacgaagtgc tcggcagcct gaccgttgac
     3361 cgcgtgcaac aatatgcttt gactgacgac gac
//
LOCUS       DASSWZ010000264         4805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_703429, whole genome shotgun sequence.
ACCESSION   DASSWZ010000264 DASSWZ010000000
VERSION     DASSWZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703429"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VFN58_06380"
     CDS             complement(<1..1257)
                     /locus_tag="VFN58_06380"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8917652.1"
                     /translation="MISAEENNLLTQIGPETPCGGLLRRYWQPVALTEELPEGGAPVK
                     VKILSEELVLFRDDQKRPGLLGLHCSHRGTDLSYGRVEDGGLRCLYHGWLYDVAGRCL
                     EQPGESGGGEHRDAIRHPAYPCTEAGGVIFAYMGPGAPPLLPNYDFLTAPEEYRTVVK
                     IFHNCSYLQGNEGNIDPVHLSFLHQYLSEAQVARQRIVPGSNATDNTLLGNDIAPTIE
                     VEIKDFGLRIYTLRNAGPDKRYLRVTNFVMPNLAAFGGSTVGDGYAVHWHVPIDDTHH
                     WKYIFMFSRSKALDDELRNKSRAELTPDYRLTRNAANRFQQDRASMKTQTFTGMGINF
                     QAHDAFATESQGPVQDRTNEHPVSSDKAIIAARKLLLNAIKDVREGRQPQHVIRDASS
                     NRFSHLVVHSELVPAAADLKQYVKELE"
     gene            complement(1367..2377)
                     /locus_tag="VFN58_06385"
     CDS             complement(1367..2377)
                     /locus_tag="VFN58_06385"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917653.1"
                     /translation="MKSKRDLVFLILILLLAFQISHAQEKKLDPFTISYASVSGTRGP
                     LWIARDLGLFAKYGLDVDLVYIASGVTSVNALLGGSVDIIAASGSSVVSAASRGAPLV
                     IIASLGHIAYKLVALPSITNVQGLKGKIIGSSRIGAGTDYALKRLLPKLGLTPGKDVQ
                     LIPTGISESDRRLVMMLQGKIDATLATEDNLLQLGARGTKLSVLADLYDNGVYTTGSD
                     IATSRQLLKERPRQLKAFIMALTEGTAYGRANKDQTMRIYRKYLKIEDPKLLESIHKN
                     YLLGTIPEKPYPREEALQNDIEDLSYTYADLKGRRAAEFLDLSLLKSLEDEGFFKRLY
                     GK"
     gene            complement(2497..3192)
                     /locus_tag="VFN58_06390"
     CDS             complement(2497..3192)
                     /locus_tag="VFN58_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8917654.1"
                     /translation="MEGLQVVTGEGVLSGSGHLSVRIPGTETFFINPRFAGILADPKD
                     ICAVNFAGKRIAGKGPIPSETPIHAAVYRSRPDVASVLHCHARYSVLVGLLESGLIPF
                     NREARIFADGVPIFPESRGINSFSLAQRMVDALGPHYAVFLRGHGIVVVGPGIEGTCL
                     SAIQLERACQDQLLMMSITTVKPMADAGRGRNNARLENPYRAWPFLLYKHKVKSKAQI
                     RAGIRTLEEGEHY"
     gene            3531..4574
                     /locus_tag="VFN58_06395"
     CDS             3531..4574
                     /locus_tag="VFN58_06395"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917655.1"
                     /translation="MASILYLGTDEGVVTLKSEDGRSWKVEHHGLKDWEVPEVAVSPS
                     APNKVFAGTRGDGVWLSEDFGATWSKPCYGKRGPGKVRCLTIDPRDPNTIYAGTEPID
                     VFISRDGAKSWKRLDSVWNIPSVSTITYPVATVEPHVRDITIDPKEPNTMYVALQVGY
                     MIKTKDGGATWELLNRNLDCDVHTIVLHPEDSNKIFIATGGHDCRAGKAPGRALYYSS
                     NGGESWAPMAAEFKEEYSVPLTIHPKRPDVIYSAVANGQPGQWRKRASGAEAFLIQSL
                     DAGKSWKKLDGEVSRANQSFVEAFAFDPVNFDRMYAAQQNGDIFGSENSGASWFKLNV
                     KAPHVSDMKAARA"
BASE COUNT         1074 a         1350 c         1288 g         1093 t
ORIGIN      
        1 ctcgagctcc ttgacgtact gcttcaagtc cgccgcagcg ggaacgagtt cggaatggac
       61 caccagatgc gagaagcggt ttgagctcgc gtccctgata acatgttggg gttggcgtcc
      121 ctcccgcaca tctttgatgg cattcaacaa caatttgcgc gcagcaatga tggccttgtc
      181 cgaagatacc ggatgctcgt tggttcggtc ttgcactggc ccttgactct ccgtggcaaa
      241 ggcatcgtga gcttgaaagt tgatccccat gccggtgaaa gtttgggtct tcatcgacgc
      301 gcgatcctgc tgaaagcggt tggccgcgtt gcgcgtcaag cgatagtccg gtgtaagctc
      361 ggcgcggctt ttattgcgca gctcgtcgtc cagagccttg gatcggctga acatgaaaat
      421 gtatttccaa tggtgagtat cgtcgatagg cacatgccag tgaacggcgt aaccatcccc
      481 caccgtcgag ccgccgaagg cagccaggtt aggcatgacg aaattggtga cccgcaaata
      541 gcgcttatcc ggtccggcat ttcttagcgt gtaaatcctc agtccaaaat ccttgatctc
      601 cacttcgata gtgggagcga tatcgtttcc cagcagagtg ttgtctgtag cattgctccc
      661 tggcacaatc cgttgccgtg cgacctgagc ctcgctcaaa tattgatgca ggaaagaaag
      721 atggactggg tctatgttgc cctcgttgcc ctgcaggtag ctgcaattgt ggaaaatctt
      781 tacgaccgtc cggtactctt cgggcgccgt caaaaaatca tagttcggaa gcaggggcgg
      841 cgccccaggg cccatgtaag caaagataac tccgccggcc tccgtgcagg gataagccgg
      901 atgacgaatc gcatccctat gctcgccgcc gccggattcc cccggttgct ccaaacagcg
      961 tcctgcaaca tcatagagcc aaccgtgata aagacaccgg agtcccccgt cttcgactcg
     1021 tccataactg agatccgtcc cgcgatggga gcaatgcagc cccaaaagtc ccggacgctt
     1081 ttgatcgtcg cggaatagga ccagttcctc gctgagtatc ttaactttga ccggcgctcc
     1141 gccttcgggc agctcctcag tcagagccac tggctgccaa taccgccgca gcagcccgcc
     1201 gcagggagtt tcaggaccga tttgggtgag taaattgttc tcttccgcag agatcatctt
     1261 gatgtagtga atactacttc aacattgcta cggcaagacc gccatgaaca gtgcttatgc
     1321 gccatctctc atagcctgca tggttctgag tttgctgcga ccagaattat ttcccgtaaa
     1381 gccgtttgaa gaatccttcg tcttccaagc tcttcagcaa ggagaggtcg agaaactcgg
     1441 cggcccttct tcccttgaga tcggcataag tgtaactcag atcctcgatg tcgttttgga
     1501 gcgcctcttc gcgcggatag ggcttctcgg ggatcgtccc cagtaaataa ttcttatgga
     1561 tcgactccaa caacttcgga tcctcaatct tcaagtactt gcggtaaatc cgcatggttt
     1621 gatccttatt tgctcggcca taggcggttc cctcagtcag cgccatgatg aaagccttca
     1681 gctgccgcgg ccgctccttc aggagttgtc ggctcgtcgc gatatcgctg ccggtcgtgt
     1741 agaccccgtt gtcgtaaaga tcagcgagca cggacaactt cgttcccctg gcgccgagtt
     1801 gaaggagatt gtcctccgtg gccaaggtgg cgtcgatttt accttgcagc atcattacga
     1861 gtctgcggtc tgattcgctg atgcccgtgg gaatcagttg cacatcttta cctggggtca
     1921 gacccaattt cggcagaagc cgtttcagtg cgtagtcggt gccggcgccg atgcgacttg
     1981 aaccgatgat cttgcctttg aggccttgta cgttggtaat cgacggcagc gcaactaatt
     2041 tgtaggcgat gtggcccaaa ctggcgataa tcactagagg cgcgccccgc gaggcggcgc
     2101 tgaccactga cgatcccgaa gcagcgatga tgtcgacgct gccgcccagc aaagcattga
     2161 ccgatgtcac acccgaggcg atataaacga gatcgacatc aagcccgtac tttgcaaaaa
     2221 gaccgagatc tcgagcgatc cagagcggac cgcgtgtgcc cgagaccgac gcataggaaa
     2281 tggtaaaagg atcaagtttc ttttcctgtg catgagatat ttgaaacgct aacagcaaaa
     2341 tcaggatcag aaacaccaaa tctcgcttgc tcttcatctc tcttactcgt ttcttttttg
     2401 gtctttgtct ccagttttca tgtgctctcc ttgtctcccc gtctccttgt ctcctgtctc
     2461 cctgtctatc tgtctcctac tctctatcgc tgtatttcag tagtgctcgc cttcttcgag
     2521 cgtgcggatg ccggcgcgaa tttgcgcttt cgatttcact ttatgcttgt acaaaagaaa
     2581 gggccatgcg cgatagggat tctccaagcg cgcgttgtta cgccctcgac ctgcatcggc
     2641 catgggtttg acggttgtga tactcatcat caggagctga tcctggcagg cccgttcgag
     2701 ctgaatcgcg gatagacaag tcccttcaat tccagggccc accacgacga tgccgtgccc
     2761 gcggagaaaa accgcataat gcggtcccaa agcatccacc atccgctgcg ccaggctgaa
     2821 actgttaatg ccgcggcttt cgggaaagat cggcacgcca tcggcaaaga tcctcgcctc
     2881 tcgattgaaa ggaatcaaac cgctttcaag caagccgacc agaaccgaat aacgcgcgtg
     2941 gcagtgaagc acacttgcga catcggggcg gctccggtaa accgcggcgt ggatcggcgt
     3001 ctcggaagga atcggccctt ttcccgcaat gcgtttgccg gcgaagttca cggcgcaaat
     3061 gtcttttgga tcggccaata tgccggcgaa tctgggattt ataaaaaagg tttcggttcc
     3121 agggatccgc acgctcaagt gacccgagcc ggagagtact ccttcaccgg tcacgacctg
     3181 caaaccttcc accagtcgcc gcttcagatt ttcgatcgtt cggcggagtc taccctgagc
     3241 actgccgaag ggctcagcac aggttttcgg ttttggactt gcgattagcc accccctcga
     3301 cggttatcgg cttactaaca cgcgggtata tccgcgtcaa cgtacttctt tgcgacctca
     3361 atgtaatgaa tcccgagggg aaagttctca ctgcaggctc acccctcgat cgacacagtg
     3421 cagtagtctt ttgaactgct cggaccgttc aaggttgacg gcctaaatcc ggttgtcgcc
     3481 ggtatagttc tgtagtagat tcgcactcaa atagaaggga ggatatggtg atggcatcga
     3541 tactttactt gggaacagac gaaggcgtgg tgacacttaa aagtgaggat ggccgttctt
     3601 ggaaagtaga acatcacggg ctcaaagatt gggaagtccc agaggttgcg gtctcgccat
     3661 cggcgcctaa caaggtgttt gccggaaccc gcggcgacgg cgtctggcta agtgaggatt
     3721 tcggcgctac atggtcgaaa ccctgttacg gcaagcgggg tcccggaaaa gttcgctgcc
     3781 tcaccattga tccccgcgat cccaatacga tttacgcagg caccgaaccg atcgatgtct
     3841 tcatcagccg ggacggcgcc aaaagctgga agcggctcga ttctgtttgg aatattccgt
     3901 cggtatcaac catcacctat ccagtggcca ctgttgagcc tcatgtgcgt gacatcacca
     3961 ttgatcccaa agagcccaat acaatgtacg tcgccctgca agtgggctac atgatcaaga
     4021 ccaaagacgg cggcgccact tgggagcttt tgaataggaa tctcgattgc gatgtacaca
     4081 ccattgtgtt gcatccggag gatagcaaca agatcttcat tgccaccggt ggccacgatt
     4141 gccgggcagg caaggcccca gggcgcgcgc tctactacag cagcaacggc ggcgagagct
     4201 gggcgccgat ggccgcagag ttcaaggaag aatattcagt cccgctgaca atccacccca
     4261 aaaggcccga tgttatttat tccgcggttg caaacgggca gccgggacag tggcgaaagc
     4321 gtgcgtcggg cgcggaagcg ttccttattc aatccttgga tgccggcaag agctggaaaa
     4381 aactcgacgg cgaagtttca agagccaacc agagcttcgt ggaggccttt gcctttgacc
     4441 cggttaactt cgaccgaatg tacgcggcgc agcaaaacgg cgatatcttc gggagcgaaa
     4501 acagcggcgc cagttggttc aaattgaacg taaaagcgcc gcatgtctcc gatatgaagg
     4561 ccgctcgcgc ctaagaacgg ttacgcgtct agagtttcga gttccgggtt tctgaattcc
     4621 caactcgaga ctccagactc gaaacccgag actaaaacca tggcaaactt agtcggctgc
     4681 ttggcgatgt cccatgcgcc ccagctcatg ctcaaccccg atcattgggg cttactcaac
     4741 acccgcgaga aggagcggct acccgacaaa ccggagttgg accccgaaac ggcagaggtg
     4801 aaatg
//
LOCUS       DASSWZ010000265         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_94804, whole genome shotgun sequence.
ACCESSION   DASSWZ010000265 DASSWZ010000000
VERSION     DASSWZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94804"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..257)
                     /locus_tag="VFN58_06400"
     CDS             complement(66..257)
                     /locus_tag="VFN58_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET8917656.1"
                     /translation="MFGLGIGELLIILVIVVVLFSRRLPDLGESFGAAIRKFRKAVKE
                     PDEIDITPKQNSGRNDPKK"
     gene            350..1144
                     /locus_tag="VFN58_06405"
     CDS             350..1144
                     /locus_tag="VFN58_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET8917657.1"
                     /translation="MASLNTPLVRFFVASLTLHLFLLLVWGRPSIRTRPQESIAVSLL
                     PPPPPEESQTQPSPAPSEAPRRPSKAPVKIAKKNSPPLREKTAIPKEKPAIAREIPEL
                     KEPTREAARPPAREQIPDKAIVTERPLPTLKELLPPVGWSADARASGANGPVRLDTKD
                     PQYVTYFNSIKRAIEVVWQYPELALRYGLQGRLLLEFSILGNGDIESAKIVRSSGSNL
                     LDEEALRAVKAAAPFGPIPAWIGKNRIDIVASFEYLDNRLNYRFMR"
     gene            complement(1174..>2023)
                     /gene="gcvPB"
                     /locus_tag="VFN58_06410"
     CDS             complement(1174..>2023)
                     /gene="gcvPB"
                     /locus_tag="VFN58_06410"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET8917658.1"
                     /translation="DVAAIMITNPNTLGLFEKDIEAIAAVVHAKGGLVYLDGANLNAL
                     MGVAKPGHMGVDVLHMNLHKTFSTPHGGGGPGAGPVAVKESLSEYLPVPRIVKQDDRF
                     KLLEDCPKSIGRVRSFFGNFGILVRAYTYIISLGGEGLEESSRMALLNANYIRKNLEK
                     DYQIAYSEPCMHECIFTDRLQHKYGVNTLDIAKRLLDYGFHPPTIYFPLVVSGALMIE
                     PTETETPETLDAFVEAMHAIAQEAKENPDIVKTAPHSTPVRRLDEARAARKPILRWDP
                     SQVGAE"
BASE COUNT          491 a          562 c          509 g          461 t
ORIGIN      
        1 tcaacgaaag aaacaaagga cttcagctgc gaagtcctga cagccgaagg agaagcgtag
       61 aggagttact ttttagggtc gtttctgcca gagttctgtt ttggagtaat atcgatctcg
      121 tccggttctt tgacggcctt gcgaaatttc ctaattgcag cgccaaagct ttcgccgagg
      181 tcgggtaacc gccggctgaa cagcaccacg acaatcacga gaattatcag caactcaccg
      241 atcccgaggc cgaacatgta tcctccttct gaacaatcct aattgacctg ggctttcatt
      301 tgcaacccat tatttgctaa gcatgtgggt cgtgcagtag tatgtttgtg tggcttccct
      361 gaatactccc ctagtgcgct tctttgtcgc ttccctgacc ctgcatcttt tcttgttgct
      421 agtgtgggga agaccttcaa tccgcaccag accccaagag tctatcgctg tttctctact
      481 cccaccgccg ccaccggagg aaagccaaac tcaaccgtct cctgctccga gcgaagcccc
      541 gcgccgacca tcaaaggcac ccgttaagat cgccaagaag aactctccgc ctctccgaga
      601 gaagactgcc atcccgaaag agaaacccgc catcgcgcgg gaaattcctg aattaaaaga
      661 gccaacccgc gaagcagctc gtcctccggc gcgagaacag ataccggaca aggcgattgt
      721 caccgagcgg cccctgccga ccctcaaaga actactcccc ccggtgggtt ggtcggcaga
      781 cgcgagagcc agcggcgcta acgggcctgt ccgcctggat acaaaagatc ctcagtatgt
      841 aacctatttt aacagcatca agcgggcgat cgaagtggta tggcaatacc cagagctcgc
      901 gctcaggtat ggattacagg gaagacttct gttggaattc tcgattcttg gcaacgggga
      961 cattgagagt gcaaagatcg tgcgctcttc ggggtctaac ttgctcgacg aggaagcact
     1021 acgcgccgtc aaagctgccg cgccatttgg ccctatacca gcgtggattg gcaaaaaccg
     1081 aatcgatatc gttgcaagct ttgagtactt ggacaaccgc ttgaactacc gtttcatgcg
     1141 ttaaactggc ggcaattgcc atgcaatcga ggtttattcg gctccgacct gcgaaggatc
     1201 ccatcttaga atcggttttc tcgctgctct agcttcatcg agacgtctga ccggcgtcga
     1261 gtgcggtgca gtcttgacga tatcgggatt ctctttggct tcctgagcaa tggcgtgcat
     1321 tgcttcaaca aatgcatcca atgtttccgg agtctcggtt tcggtgggtt caatcatcag
     1381 tgcaccggaa acgaccaaag gaaaatagat cgtaggcggg tgaaacccat aatctagaag
     1441 acgcttggcg atgtcgagag tgttgactcc gtatttgtgc tggagccgat ccgtaaaaat
     1501 gcattcgtgc atgcacggct cactataagc gatctgatag tccttctcca aattcttgcg
     1561 aatatagttc gcgttgagga gtgccatccg gctcgattcc tcaagacctt ctccaccgag
     1621 agagataatg taggtgtaag cacggaccag gataccgaaa ttaccgaaga aggaacgcac
     1681 ccgcccaatc gacttagggc aatcctcaag caacttaaac ctgtcatctt gcttgacgat
     1741 ccgtggcacg ggcaaatatt cagacaggct ttctttgacc gccacgggac ctgcgcccgg
     1801 accacctccg ccatgcggcg tggagaaggt cttgtggagg ttcatatgaa gaacgtcaac
     1861 gcccatgtgg ccgggtttcg ccacacccat caacgcgttc aagttcgcgc catcaagata
     1921 aaccaagccc cccttcgcat ggacgaccgc agcgatagcc tcgatgtcct tttcgaaaag
     1981 gccaagagta ttaggattgg taatcattat ggcggcgaca tcc
//
LOCUS       DASSWZ010000266         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1028853, whole genome shotgun sequence.
ACCESSION   DASSWZ010000266 DASSWZ010000000
VERSION     DASSWZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028853"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFN58_06415"
     CDS             complement(<1..194)
                     /locus_tag="VFN58_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917659.1"
                     /translation="MIGIVRHFKVVGKPHPRLDGPDTVTGRATYTVDVVLPSMLQAKL
                     FRSSVPHAKIQRLDVSRARAL"
     gene            complement(191..592)
                     /locus_tag="VFN58_06420"
     CDS             complement(191..592)
                     /locus_tag="VFN58_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917660.1"
                     /translation="MITTTGIYHIGIPVNDVERAVKFYTEVLGMKVAKLNRDDMGDHL
                     NRADLRSGNGMVVLFQRPTPIEKDALKEDGATHHAFTVSPEDFELAAQKMKDWGVQVH
                     SIPTVDRPSGRGFYFFDPDGNLLQLYAPPKN"
     gene            761..1882
                     /locus_tag="VFN58_06425"
     CDS             761..1882
                     /locus_tag="VFN58_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917661.1"
                     /translation="MNKSLLALMFVAVVITPSNIHLASAAEVRRPPEWDKTLEAAKKE
                     GKIVIAIPPANELRKEMETVLKQKFGLEAELVSAPGPRNASRIAAERKAGVSYFDALI
                     VGTGTAVGLAHEGMLEPIEPFLILPEVKDPKEWWGGHIWEDNLSTNRYLYSFLADVST
                     HSIWYNSSLAKPEELRSFDDYLSPKWKDKIGFSDPRVPSSGQSVWSFMWEVKGEEFLK
                     KLVQQELFLTRDLRQLTDALAKGKVALAFGIGRSQAEPFVKAGLPIKPAPAPREGLPA
                     SNSFGVLGIVKDPPHPNATRIFINWFLSREGQDWYGRVMQNGSRRLDVDTKWLKELGV
                     DAAKDSITLQEYHRVRNHLEDKYTKVRMPSGKFAETILK"
     gene            2039..>2919
                     /locus_tag="VFN58_06430"
     CDS             2039..>2919
                     /locus_tag="VFN58_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917662.1"
                     /translation="MTIKGRDLSIWIAAVVFAITVAAEIAPQQYVAASVEDIAMMKSA
                     DRQKILIEGAKKEGKITWYTTLIVDQVVRPVKEAFEKEYPFIQIDFYRGNAENLVRRM
                     LAEYQAKRYDVDLIDGTVSPVMVQRAGLLQRFYSPFFAEYPAELKDSQGFWGSTNLYF
                     LTPGYNTRMVKLNELPKTYQDLLNPRWKGQMMWSTSRGSGAPIFIGNVLISMGQEAGK
                     AYLQKLKGQNIAKSTASNRQILDLTIAGEFPIALHIFNHHAYISKTAGAPVDWYAIEP
                     VTATIQTIALAKSTPHP"
BASE COUNT          688 a          782 c          822 g          627 t
ORIGIN      
        1 agcgcgcgcg cccggctcac gtcgaggcgc tggatctttg cgtgagggac ggaactacga
       61 aaaagcttgg cctggagcat cgacggcaag acgacatcca ccgtataggt ggcacggccc
      121 gtcacggtat ccgggccgtc caggcgcggg tgcggttttc cgacgacctt gaaatgtctc
      181 acgatgccga tcaatttttc ggtggcgcgt agagctgcag aaggttgccg tccggatcga
      241 agaaatagaa tcctcttccg ctgggacgat ccaccgtagg gatgctatgg acctgaactc
      301 cccaatcttt cattttttgt gccgccaact cgaagtcttc cggggacacg gtgaaggcgt
      361 ggtgggtggc gccatcctct ttcaaagcat ctttctcgat cggcgtaggt cgctggaaga
      421 gtacgaccat gccgtttccc gatcgcaggt cggctcggtt cagatgatct cccatgtcgt
      481 cccggttgag ctttgcgacc ttcattccca aaacctcggt gtagaatttt acggcgcgtt
      541 ccacgtcatt gacggggata ccgatatggt aaattcctgt cgtggtaatc atgattcctc
      601 ctgcgtaaat acctttggga cgcttgaacg ttgtcaatgc caattaccac cggaagattc
      661 ggcgagtcaa ccgcttgtgt tctagactgg aagccgcctt gacactccat ggggttttca
      721 attacagctc agcaaaagaa atcctctctt cggggacttt atgaacaaaa gtttacttgc
      781 tctgatgttc gttgccgtcg tgatcacgcc ctcaaatatt cacctggcct cagcggcgga
      841 agtgcggcga cccccggagt gggataagac tctggaagcc gcgaagaaag agggcaagat
      901 cgtcatcgct attccgcccg ccaacgagct cagaaaagaa atggagacgg tcttgaagca
      961 gaagtttggc ctcgaagccg agcttgtctc cgccccgggc ccgcgcaacg caagccggat
     1021 cgctgcggag cgcaaggcgg gtgtgagtta tttcgatgcc ctgatcgtcg gcaccggcac
     1081 ggccgtgggt ctcgcccatg agggcatgct cgagccgatc gagccctttc tcattctccc
     1141 cgaggtcaaa gatccgaaag agtggtgggg tggccatata tgggaggata atctcagtac
     1201 caatcgctac ctctattcct tcttggccga tgtgagcacc cacagtattt ggtacaactc
     1261 gagcctggcg aagccggaag agctccgttc ctttgatgac tatctctcgc ccaaatggaa
     1321 agataagatt ggcttcagcg acccgcgcgt gccgagctcc ggtcaatcgg tctggtcctt
     1381 tatgtgggaa gtcaagggcg aagagtttct caagaaattg gtccagcaag agcttttctt
     1441 gacccgagat ttgcggcaac tcactgacgc tctggcgaag ggcaaggtcg ctctagcgtt
     1501 cggcatcggc aggagccaag ccgagccttt cgtcaaagca ggcctcccga tcaagccggc
     1561 gccagcgccc agggaaggct tgccggccag caacagtttc ggtgtgctgg gtatcgtcaa
     1621 agatccgccg catccgaatg ccacgcgaat tttcatcaac tggttcctga gccgagaagg
     1681 gcaggattgg tacggcagag tcatgcaaaa tggctcccga cgactcgacg tagacacgaa
     1741 atggctgaaa gagctcggcg tggacgcggc aaaagattct atcaccctcc aggagtacca
     1801 ccgggtgcgc aaccacctcg aagacaaata caccaaagtc agaatgccgt caggaaagtt
     1861 tgccgaaacg atccttaaat aggcgctgtt acgagtcggc agccgtcacg gcgatgatag
     1921 aatttctcgt cgcggtccaa gagtggcata ttaaccttga caaggcgcag gctccgggaa
     1981 tagcttcgcg ttacggacaa ggcaacctgt atcaaaagcg cactcaagga gagtgagaat
     2041 gacgatcaaa ggtagagatc tatcaatttg gatcgccgca gttgtttttg caatcacggt
     2101 cgcggcagag attgccccac agcagtatgt ggcggcatcg gtggaagata ttgccatgat
     2161 gaaatcggcg gaccggcaga agatcctgat cgagggcgcc aagaaggaag ggaagataac
     2221 gtggtacacc acattgattg tcgatcaggt ggtgcggccg gtgaaggagg cttttgagaa
     2281 agagtatccc ttcatccaaa ttgattttta tcgcggcaac gccgagaacc tggtgcgcag
     2341 aatgctcgcc gagtatcagg cgaaaaggta cgacgtcgat ctcatcgacg gtacggtgtc
     2401 tcccgtcatg gtccagcggg ccggcctcct gcagcgcttt tattccccgt tttttgccga
     2461 atatccggcc gagctgaagg attctcaagg tttctgggga tcgacgaacc tttacttcct
     2521 gaccccgggc tataatacga ggatggtcaa actcaatgag ctgccgaaga cctaccagga
     2581 tctgctgaat ccgcgctgga aaggtcagat gatgtggtcg accagccgtg gctccggagc
     2641 accgatattc atcggcaatg tgctcatttc gatgggacaa gaagcaggca aggcgtatct
     2701 gcaaaaatta aagggtcaga atatcgccaa gagcactgca agcaaccgac aaattctcga
     2761 cctgaccatc gccggggaat ttcccatcgc gctccatatc tttaaccatc atgcgtatat
     2821 cagtaaaaca gcgggcgcgc cggtggattg gtatgccata gagccggtga ccgccaccat
     2881 ccaaactatt gcgttggcaa aaagtacgcc tcaccccca
//
LOCUS       DASSWZ010000267         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1014948, whole genome shotgun sequence.
ACCESSION   DASSWZ010000267 DASSWZ010000000
VERSION     DASSWZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014948"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..247
                     /locus_tag="VFN58_06435"
     CDS             38..247
                     /locus_tag="VFN58_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917663.1"
                     /translation="MAKYLQCSECGKLAFYYDDFVGTEKPFYKRFMRYPEGETKYHPP
                     RCFSCGGVPRLIHELIKEEKPAAKH"
     gene            complement(394..3096)
                     /locus_tag="VFN58_06440"
     CDS             complement(394..3096)
                     /locus_tag="VFN58_06440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET8917664.1"
                     /translation="MANLSWHSMGIDAVLKELDTAPHQGLAEDEVRRRLEKYGYNELK
                     KEEKVSHLILFFNQFKNTLIIILLIATVLSALLGEIVDASIIMAIIIFCAVLGFVQEY
                     RAEQALEALKKMLAPTITALRGGKEHEVPSKELVPGDILLLEAGDKLPADGRLIESHS
                     LKCDEAPLTGESFPVEKELKPLPEDVPLGDKKNMVFTGTTVTYGRAKAAVTSTGMSTE
                     FGKIAEEVAGVAKEETPLEKRTAEIGKWLGIICLGVCFLAAGIGIIKQLMVGKFDLQF
                     MITMVVFAIALAVAAVPEALAAIVTGALAIGMHEMAKRNALVRKMPAVETLGCTTVIC
                     SDKTGTLTKGEMTVRRIFDGERMIDVSGAGYVPEGGFSTAVNDPSTQWLLKAGLLCSD
                     AALVEDGGRWSVKGDPTEGAMVVLAVKAGLHQEETRLECPRIEEFPFSSERKRMTTIH
                     TMEDGKRVAFVKGAPEVLLERCSSLLDGGERRALNDDTRVKILRVNEEMAKDALRVLG
                     VGYKELPKMDGYGEDFVEKDLAFLGLVGMMDPPREEAIEAVKVCRQVHIRPVMITGDH
                     KLTAVAIAKELGIYHEGDLVLTGEELSKLDDQEFERMVKRVTVYARVSPMDKLKIIRA
                     WKEQGEVVAMTGDGVNDAPALKHADIGIAMGITGTEVAKEASDIVLGDDNFATIVRAI
                     ERGRWIYDNIKKYLVYLLRANITEVVVLGGVVMITDPRFLPLLPAAILYINLATDGPP
                     ALALGVAPPDPDIMQRPPRDPKESVFSWDVRTLILLGVVVECPIFLWVFFRNFSNLQL
                     ARTKIFFMYVIIELILALNFRSLKYSIFQAPPHKWLLLSIVGTLAFTAALIQVPAVLE
                     AFGIALPAASDLGMIVALGLFVTITIEITKAVLRAKGVTRAISP"
     gene            complement(3285..3485)
                     /locus_tag="VFN58_06445"
     CDS             complement(3285..3485)
                     /locus_tag="VFN58_06445"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET8917665.1"
                     /translation="MSLAPKLIPLGILGYLILPVDLLPDVFQGLGRLDDLVVLLLGLR
                     LFLRLCPPEVVQEHVKAIATGR"
     gene            complement(3698..>4567)
                     /locus_tag="VFN58_06450"
     CDS             complement(3698..>4567)
                     /locus_tag="VFN58_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HET8917666.1"
                     /translation="VGWAAVVITVWLKSGKRGIALEPEHKIEIFYLGLATIYSFIIPF
                     KGRLDLIDATVLVTIFAFYMRAAARASHVEPELEGPAELVESWGQRSRRFFTVAFFLY
                     SGLTIFIAAEPFAEGLLATGRTFGIEEFILVQWLAPLASESPEFIVAILFALRMQPGI
                     SMGALLSSKVNQWTLLIGMLPVVYTISAGRFGGMEMDSRQVEELFLTAAQSLFAVAIL
                     ANLSFTLGEAILIFVLFSTQLLVPDPRFRYYYSFMYIVLAVGWVLFNKGARQGVVGLF
                     SRAPSPQAVKITE"
BASE COUNT         1062 a         1356 c         1160 g          989 t
ORIGIN      
        1 taactcacta atctagtaag cagaaacgag gagtacgatg gcgaagtact tacaatgcag
       61 cgagtgcggc aagctggctt tttattacga tgacttcgtg ggaacggaaa agcctttcta
      121 caaacgcttc atgcgttacc cggagggcga aacaaaatac catccgccgc gctgtttcag
      181 ctgtggcggt gtgccgcgac tcattcacga attgatcaag gaagaaaagc ccgccgctaa
      241 acattaagcg ccacagcaat ggcgtcccta acgtccggcc accatcgacc gagtgtgctc
      301 ctacttttag catgctgggc aaggattctg gttctcgcgg acctgctcag atttttttcc
      361 cgcgcgatat atgagcgctg cccggccgct gagctatgga gagatcgctc gcgtcacccc
      421 ctttgccctc agaacagctt tcgtaatctc gatcgtgatc gtgacgaata agcccaaggc
      481 cacaatcata cccaggtccg aggcagctgg cagggcgatg ccaaacgcct cgagcaccgc
      541 aggaacctgg atcaaggcag cggtaaatgc cagggtgcct acaatggaga gcaggagcca
      601 tttgtgaggg ggcgcctgga agatgctgta cttgagcgag cggaaattga gggcgaggat
      661 cagctcgatg atcacgtaca tgaagaagat ctttgttctt gcgagttgca aattagaaaa
      721 gtttcggaag aaaacccaca ggaaaattgg gcactcgaca actaccccca acaggatgag
      781 ggttctcacg tcccatgaaa agacgctctc tttcgggtcc cgtggaggtc tctgcataat
      841 gtcggggtcg ggaggcgcca cacccagcgc caacgccggg gggccatcgg tggctaaatt
      901 gatgtagagg atcgccgctg gcaggagagg caaaaacctg gggtccgtga tcatgacgac
      961 cccaccgagg accaccacct cggtgatgtt cgcccggagc aggtagacga gatacttctt
     1021 gatgttatcg tagatccatc ttccccgctc aatggcccgc acgatggtgg caaagttatc
     1081 gtcgcccagg actatatcgg aagcctcctt ggctacctcg gtgcccgtga tccccatggc
     1141 aattccgatg tcggcatgct tgagggctgg ggcgtcgtta accccgtcgc cggtcatggc
     1201 aacgacttcg ccctgctcct tccacgccct gatgatcttc agcttgtcca tgggcgagac
     1261 ccgagcatag acggttactc tcttgaccat gcgttcaaac tcctgatcgt cgagcttgga
     1321 gagttcctcg cccgtgagca cgaggtctcc ttcatggtag ataccaagtt cctttgcgat
     1381 ggcaacggca gtgagcttgt gatcccctgt gatcatgacg ggtctgatat gcacctgccg
     1441 acagaccttc acagcctcta tggcctcctc cctcggcgga tccatcatcc caactaagcc
     1501 gagaaatgcc aggtcttttt caacaaaatc ctctccgtac ccgtccatct tggggagttc
     1561 tttatagccg acgccaagga cccttaaggc atccttagcc atctcttcat tgactcttag
     1621 aatctttacc cgcgtgtcat cgtttaacgc cctccgttca cctccatcta acaatgagga
     1681 gcatctttcc aggagcactt ccggagcccc tttcacgaag gccactctct tcccatcttc
     1741 catcgtatgg atagtcgtca tccgctttct ctcagagctg aatggaaact cctcgatcct
     1801 gggacattca agccgcgtct cctcctgatg caaccccgct ttgacggcaa gcacgaccat
     1861 ggcgccctca gtcggatccc ctttcactga ccatcttcca ccatcctcca ccagcgcggc
     1921 atcgctgcaa aggagtcccg ccttcaaaag ccactgggtc gatggatcgt tgaccgctgt
     1981 gctgaaacct ccttcgggga cgtagccggc accgctcaca tcaatcatcc tctccccgtc
     2041 aaagatcctc ctgaccgtca tctccccctt ggtgagcgtc ccggttttgt ccgagcagat
     2101 caccgtggtg cagccaagcg tctcgaccgc cggcattttc cttacgaggg cgtttctctt
     2161 ggccatctca tgcatgccga tcgcaagggc tccggtcacg atggcggcaa gtgcctccgg
     2221 aaccgccgct accgcaaggg cgatcgcaaa caccaccatg gtgatcataa actggagatc
     2281 gaactttccc accatcaatt gcttgataat gccaatgccc gcagctaaaa aacagacgcc
     2341 caggcagatg attcctagcc atttcccgat ctctgctgtc cttttttcca gcggcgtttc
     2401 ttctttggcg actcccgcca cctcttcggc gatcttgcca aactctgtac tcatacccgt
     2461 ggatgtcacc gctgcctttg ctctcccgta agtcacagtg gtccctgtga aaaccatgtt
     2521 tttcttgtct cccaagggca catcctcagg cagtggcttg agctcctttt cgacggggaa
     2581 ggattcccct gtcagcggcg cttcatcgca tttcaacgag tggctttcga tcaaccgacc
     2641 atcagcaggg agtttgtccc ctgcttccag gagcaggatg tctccgggga caagctcttt
     2701 tgatgggacc tcatgttcct ttccgcccct gagggctgtg atcgtagggg cgagcatctt
     2761 cttcaaggcc tccaaggcct gctcggcacg atactcttgg acaaagccga ggacggcaca
     2821 gaagataata atggccatga tgatgctggc atccacgatt tccccgagaa gcgcggagag
     2881 gaccgtggcg attagaagga tgatgatgag agtgttctta aactggttga agaataggat
     2941 cagatgagaa accttctcct ctttcttaag ctcattataa ccatactttt ccaggcgcct
     3001 tctgacctca tcctccgcta aaccctggtg cggggcggtg tcgagctcct ttaggactgc
     3061 gtcgatgccc atcgagtgcc acgatagatt cgccatagga tcctcccaaa cgtcacctct
     3121 gagggtagct gcatctcctg ctggtcggtt cgtccgaagt caaacgaccc actcttagcg
     3181 aaaaaatggc cgctatgcca gagaaaacgg aagtgaagtc caaaaaggtt tctcacacct
     3241 ttttccctcc aggaaataat tatcgaggtt gcattgcgcc ggattcagcg tccggtggcg
     3301 attgccttta catgctcttg aacgacttca ggcgggcaca accggagaaa taatctgagc
     3361 cccagcagaa gcactaccag atcgtcgagc cgtccgagac cctggaaaac gtcgggcagc
     3421 aaatcaaccg gcaaaatcag atagcccagg atgcctaacg gaatcaattt cggcgccagc
     3481 gacactcgcg gatccttgaa caaacgcgag aagagtttca ggaattttga catgtgtaag
     3541 atgagttaca ggacgccgcg tgcgctaagc tgcccggcta ttccaaaggg cgtgattctc
     3601 atcgtcccta ggttcggttc cgctgcaatc cgcagcgtcg ccggaatcct ttagagccgc
     3661 gacctcgatt ttttccccag cttaatgccg caagaactca ctcggtaatt tttacagcct
     3721 gggggctcgg agctctagaa aagaggccca cgaccccctg ccgcgcccct ttgttaaaca
     3781 atacccaacc caccgccagc acgatgtaca tgaacgagta atagtagcga aagcgcggat
     3841 cgggaaccag gagttgcgtc gagaaaagga cgaaaatcag tatagcctcg ccgagagtga
     3901 aagagagatt agccaaaatc gccacggcga acagcgattg cgccgcggtt agaaacaact
     3961 cttctacctg gcgactatcc atttccattc caccaaagcg gccggcggaa atcgtgtaaa
     4021 ccaccggcaa cattccaata agcaaagtcc actggttgac cttcgatgaa agcaacgcgc
     4081 ccatgcttat tcccggttgc atgcgcagcg caaacaagat cgcgacgatg aactccggcg
     4141 actcggacgc caggggcgcg agccactgga ccaggatgaa ctcttcaatc ccaaacgtgc
     4201 ggccggtggc cagaagtcct tcggcaaaag gctcggcggc gatgaagatc gtcaatcccg
     4261 agtagagaaa aaacgcgacc gtaaagaatc gacgcgacct ttgcccccag ctctccacca
     4321 attcagccgg gccctcgagc tccggctcga catgggaggc ccttgccgcc gcacgcatgt
     4381 agaaagcaaa gatggtaacg agcaccgtcg cgtcgatcaa gtcgagccgg cctttgaacg
     4441 gaatgataaa ggaataaatg gtcgccagtc cgaggtagaa gatttcgatc ttgtgctccg
     4501 gctccaacgc gatgcctcgc ttgccggatt ttagccaaac ggtaatcacc accgctgccc
     4561 agccgac
//
LOCUS       DASSWZ010000268         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_519729, whole genome shotgun sequence.
ACCESSION   DASSWZ010000268 DASSWZ010000000
VERSION     DASSWZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519729"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..464
                     /locus_tag="VFN58_06455"
     tRNA            402..464
                     /locus_tag="VFN58_06455"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            1028..1891
                     /locus_tag="VFN58_06460"
     CDS             1028..1891
                     /locus_tag="VFN58_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917667.1"
                     /translation="MPLVSALAASHAPNILLEPGAEWEDFMNLHYSMAPQGSASRPTL
                     EQQKKLRKDAEDAFAVLRADMEQAKPDVLIVVANDQFVNFFWNNIPTFFVTIADEVKG
                     QFTRYKFHYRNHKELGRAIVRAGMDKGIDLSFGEHIELQHTQNVPLHFLLPEPKIPIL
                     PIYVNTWVDPAPTPKRCYQVGELIREVATKSPERVAILATGGLSHFPGSPRIGEIDTQ
                     FDHKLLEVMRQGKGPNLADYSVEELLKAGDSEFLNWMVVVGTVGDAKAAYTSYMPDFV
                     ATGWGFVSWKL"
     gene            2056..2301
                     /locus_tag="VFN58_06465"
     CDS             2056..2301
                     /locus_tag="VFN58_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917668.1"
                     /translation="MSCYDLSRLLFDLKMDESCVKRALADFESVMGEYDLTEEEKAAL
                     RAGDPRKLRQLGVHGMLCLYIKRLHPEFRDNIYWQQK"
     gene            2439..2501
                     /locus_tag="VFN58_06470"
     CDS             2439..2501
                     /locus_tag="VFN58_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917669.1"
                     /translation="MTIFRLTKTFPDEERFALTI"
     gene            2539..2760
                     /locus_tag="VFN58_06475"
     CDS             2539..2760
                     /locus_tag="VFN58_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET8917670.1"
                     /translation="MREAWSKRRYEGHFVSKLTDCDGENSETDSSLDFAKDCEYITVQ
                     CHTELTALSQHVGRMLGSMIKNPGPFLIR"
     gene            2896..3687
                     /locus_tag="VFN58_06480"
     CDS             2896..3687
                     /locus_tag="VFN58_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET8917671.1"
                     /translation="MDLGLKGKVALVAGASQGMGRATALGLSREGAKVSICARGESAL
                     NEAAEMIRRETGGEVMVMVADMSKPEDIRRFVNDSAQHFGRLDIVVNNAGGPPPGEFM
                     KFTDEDWNNAYNLSFMSTMRMTREAVPHMRKVGGGRVINITSYAVKEPIAGLVLSNAV
                     RSAVIGLAKTLSRELGRDNILINNVCPGRIDTDRAQKLNKARAERLKKPVEEINKEMA
                     AEVPLGRYGTAEETADLIVFLGSERASYITGTTIQIDGGLVRGIQ"
     gene            3687..4019
                     /locus_tag="VFN58_06485"
     CDS             3687..4019
                     /locus_tag="VFN58_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917672.1"
                     /translation="MVHFVIQWDLPDQKANLTIYANKAKNDWLPTTLSHPGVIEIRNY
                     RNPLEVTPHVAITIDFETLDNWRDFIDSADYIRIMRELRILGCRSFTANVWAPSKYFP
                     ESMTPEKK"
     gene            complement(4094..4363)
                     /locus_tag="VFN58_06490"
     CDS             complement(4094..4363)
                     /locus_tag="VFN58_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917673.1"
                     /translation="MSRILAIKGTEGEREVDYAALSLKEIQKRIKAYEKQYGGYAKVL
                     RGYDCESGTPEDYLVLIDWECLLAEIKSRKRAKLSLIRGGRKKPR"
     gene            complement(4360..4647)
                     /locus_tag="VFN58_06495"
     CDS             complement(4360..4647)
                     /locus_tag="VFN58_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917674.1"
                     /translation="MAETADNKNWKCLNISVTDKESGAVVEYKIHFYRKLNGGYDEVR
                     YDSHEIRRGKKTLAPHLHMKLGTPFKNVQEGEAELKRIIDGILPGLREITR"
     gene            complement(4706..>4966)
                     /locus_tag="VFN58_06500"
     CDS             complement(4706..>4966)
                     /locus_tag="VFN58_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917675.1"
                     /translation="DRDDIAYLTYFNAGLRLYDIRDARAPKEIGYFVPPDPKIRIGTK
                     PSRLVAQTEDVLVDRRGCIYISDKNLGIYILRLRAIWPYTSV"
BASE COUNT         1162 a         1284 c         1302 g         1218 t
ORIGIN      
        1 cgcgcagcca gccgaattcc ctcggcgcgg gcggagtcag tagctggatc gaccggcgat
       61 agatacccta tccgagggac tttcgtcggc tgctgcgcct cggctgggaa gccaagagcg
      121 ataagcatca tgccaagacc aaagctaccg agtttccgct tattcatttc tccctcacat
      181 ctcacgcaac gcgcggctgt gcttgttttg tgcctgaacg tggacagaat atacacattc
      241 cattaccgaa gaaaaagaaa gcgagcgtcg gaacagccca ctgctttgaa tgtgctttga
      301 atgaactatc tgtatctatg tgtttgaatc ggtaaaatcg accggttgag actgccgtta
      361 aacgcttgaa tagataccaa tagcgaccga tagtgacgca tagtcccgct acggcaagag
      421 gactgaaaat cctcgtgtcg gcagttcgat tctgtccctg gccatactcc aacaatccct
      481 ccaaagacac tgcctacagg ctattctcga cctccttaga ccgaactttt tcgcagacct
      541 gaaagcgacc cctgcctctc ggccacccaa cgctcgtacg tttttgccac tcagtttgat
      601 attccttgtg agagccacgc gaggtcaaat gcctaaacct tcccataaca ttctctaagt
      661 gcccaaattt ttgaaacttc cgggggccaa ttgggatttg ccgattcctt ttgacccgat
      721 tccgtttcaa tcagaggatg ggtggtcggc tgggcaggtg atttttagct tttcgagacc
      781 gctgcgttcg attatcatta agaggtttta accgcttagg cgatgcgaag aagggggcgc
      841 caattatgga tcggctcggc aaatttttta atcttacact gcgttatgtc tttgaaccgg
      901 gactcaattg ggttttggag cacccgatta tatcggttgt cgttgtcgtg acgctggtgt
      961 attgggcggt aagaggatat aggattctgt aaaaggtcgt ttccaacaag aataggagga
     1021 ctgaacgatg ccgttagtct ctgcgttggc ggcaagccat gctcctaaca ttctcctcga
     1081 acctggagcc gagtgggaag actttatgaa tcttcactac agcatggcgc cgcagggttc
     1141 cgccagcagg ccgaccttag aacaacagaa aaagctgcgt aaggacgccg aagatgcttt
     1201 tgctgtgctg cgagccgata tggagcaagc aaaaccggat gtactgatcg tcgtggccaa
     1261 cgatcagttt gtgaattttt tctggaacaa cattccgact tttttcgtaa cgatcgccga
     1321 tgaggtcaag ggacagttca cccggtacaa gtttcactat cgaaatcaca aggaactggg
     1381 acgcgcaatc gtgcgcgcgg ggatggacaa agggattgat ttatcctttg gcgaacacat
     1441 cgagttacag cacacgcaaa acgtgccgct tcatttcctt ttgcctgaac ccaagatccc
     1501 gattctacct atctacgtga acacctgggt cgatccggcg ccgacgccta agcgctgcta
     1561 tcaagtcggt gagttgatcc gagaggtggc cacgaaatca ccggagcggg tggcaattct
     1621 cgccaccggc ggtctctcgc attttcccgg ctcaccacgc atcggtgaga tcgatacgca
     1681 attcgatcac aaactcttgg aagtgatgcg acagggcaag ggacccaacc tggccgacta
     1741 ctccgtcgag gaactgttaa aagcgggaga tagtgaattt ctcaattgga tggtagtcgt
     1801 cggcaccgtc ggcgacgcta aagccgccta cacgtcgtac atgccggatt tcgtggccac
     1861 gggttggggc tttgtcagtt ggaaactcta gcaggctgct atttggctta tggcgttatg
     1921 gcttatagcg tatggctcgt atggtcaaag ctatatgcca taagctatca gccatatgcc
     1981 cgcctcgcct ctgagtcttt ttgagcaacc tgtaatcaga gttttcagga gccccccaac
     2041 attaaggagg actttatgag ttgttacgat cttagccgtc tactctttga cctcaaaatg
     2101 gacgagtcgt gcgttaagag agcgctagcg gatttcgaat ccgtaatggg ggaatacgat
     2161 ctcaccgaag aggaaaaagc ggccctcaga gctggagatc cgcgaaaatt gcgccagctg
     2221 ggcgtgcacg gcatgctttg tctttacatc aaacgtctcc atcccgagtt tcgcgataac
     2281 atctactggc agcagaagtg agagcggagg tcggaagtca gggttcggaa gtcggaggtt
     2341 cgaggtctct agtccggagt taaaattcac accgagccgt gatgaaactg aattcagcga
     2401 aagacttgga cgtgtacaag aaagcctatc agttagcaat gacgatcttc cgacttacca
     2461 aaacattccc tgacgaagag cgcttcgctc taactattta gtcaacttag acgctcgtca
     2521 cgctccgttt gcttaaactt gcgggaagca tggtcgaaaa gaagatatga gggtcacttt
     2581 gtgagcaagt tgaccgattg tgacggtgag aacagcgaga ccgattcgtc tttggatttt
     2641 gcaaaagact gcgagtacat cacggttcag tgccacacag aactgactgc gttgtcccag
     2701 catgttggca gaatgctcgg cagcatgatt aagaatcccg gcccatttct catacgctga
     2761 cttctcatct ctggtttctc tccgacacga cttctgactg cggacctccg acctctggtt
     2821 cgccttccct ttttgaccct ctgagatggg tctgatagta tccggcacac tttcaagaga
     2881 ttagggggat cgaggatgga tcttggactg aaaggtaagg tcgcgcttgt ggcgggcgcg
     2941 agccagggca tgggacgagc cactgccttg gggttgtcgc gcgagggtgc gaaggtcagc
     3001 atctgtgccc gcggggagtc ggcgctgaac gaggccgccg agatgattcg aagggaaacc
     3061 ggcggagagg tgatggtcat ggtcgcggat atgtccaagc cggaggacat caggcgcttc
     3121 gttaacgaca gcgcacaaca cttcggccga ctggacatcg tcgtcaataa cgccggcggc
     3181 ccgccgcccg gcgagttcat gaaattcacc gacgaagact ggaacaacgc ctataatcta
     3241 agcttcatga gcaccatgcg catgacccgt gaggcagtgc cacacatgcg caaggtcggc
     3301 ggcggccgcg tcatcaacat cacatcctac gccgtcaagg agcccatcgc ggggttggtg
     3361 ctgtcgaacg cggttcgcag cgcggtcatc ggcctggcca agactctgtc gcgtgaactg
     3421 gggcgggaca atattctaat caacaatgtt tgccctggtc gcattgatac ggatcgagcc
     3481 cagaagctca ataaagcccg cgctgagcgc ctcaaaaagc cggtggaaga aatcaataaa
     3541 gaaatggccg cggaagttcc gttgggacgc tatggcacgg cggaagaaac tgcggacctg
     3601 attgtgtttt tgggctcgga gcgcgcaagc tacatcaccg gtacgacgat tcaaatcgac
     3661 ggcggcttag tacgggggat tcaataatgg tgcatttcgt cattcaatgg gatcttccgg
     3721 atcaaaaagc caatctgaca atttatgcta acaaagcaaa aaacgattgg cttccgacca
     3781 cactatcgca tccgggtgtg atcgagattc gcaactaccg taaccccctc gaggtcactc
     3841 cccatgtcgc cattacgatc gatttcgaga cgttggacaa ctggcgtgat ttcattgact
     3901 cggcggacta catccgcatc atgcgcgagc tgcgcatact gggctgccgc agtttcacgg
     3961 ccaacgtttg ggcaccctca aagtattttc cagagtcgat gacgccggaa aagaaatgaa
     4021 acacgtgagt cgtctactga aggcgaaccc tctagacccc ggtgagtggt gtggagtcaa
     4081 agctattgcg gggttatcgc ggttttttcc tgccgccccg aatcagggaa agttttgctc
     4141 tttttctgct cttgatttca gctagcaggc attcccagtc gattaggacg agatagtcct
     4201 ccggtgttcc ggactcgcaa tcgtaacccc ttaggacttt ggcgtagccg ccatattgtt
     4261 tctcataagc cttgattctt ttttgaattt ccttcagcga aagagcggcg taatccactt
     4321 ctctttcacc ttcggttcct ttgatggcta ggatgcggct catcgggtta tctccctaag
     4381 tcccggcaat atgccgtcaa ttattctttt gagctctgct tcaccttcct gcacgttctt
     4441 aaacggagtt ccaagcttca tgtgcaggtg aggtgcgagc gtcttctttc ctcttctgat
     4501 ttcgtggctg tcataacgga cttcatcata ccctccattg agctttcggt agaaatggat
     4561 tttatattcg acaaccgcgc ccgactcctt atctgtgacc gagatgttca gacatttcca
     4621 atttttgtta tcggctgttt ctgccacact tttagtcttt caaacttcgt cacacgcggc
     4681 aacttcgtct acaggttacg ccaccttata ctgaggtgta aggccagatt gccctcagcc
     4741 gcagaatata gatccccaga ttcttatcgc taatataaat acagccgcgc cgatcgacta
     4801 aaacgtcttc cgtttgagcg actagccggc ttggcttggt gccgattctt atctttggat
     4861 cgggcggtac gaaataacca atctccttcg gcgcgcgcgc gtcgcggata tcgtagagcc
     4921 gcaagccggc gttaaagtaa gtcagatagg cgatgtcgtc gcggtc
//
LOCUS       DASSWZ010000269         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_364042, whole genome shotgun sequence.
ACCESSION   DASSWZ010000269 DASSWZ010000000
VERSION     DASSWZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364042"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFN58_06505"
     CDS             complement(<1..242)
                     /locus_tag="VFN58_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET8917676.1"
                     /translation="MIEVIALPLKEDSGKGHTIVEGNPAAVNAPVLETLSLTRRFGEH
                     TAVDSLTVSVKRGEVFGLLGPNGAGKTTILKMLTTLL"
     gene            942..2141
                     /locus_tag="VFN58_06510"
     CDS             942..2141
                     /locus_tag="VFN58_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET8917677.1"
                     /translation="MLIESRPPIVRYGIAVASVGLALAVASLLPYRADPSHFTLFFAA
                     VMISSWYGGLGTGLVATILSALSLDYFFIAPIHSITLDWQAFLRLGVFLVVAVVTSYL
                     TTARKRAEEALRKAHAELEERVRERTAELTRANESLRAEIVERKRAENELWGVQQEMG
                     RVERLAALGRMTGTIAHELGTPLNSVLGYTQLLAQEALSEGARRRLNIIETQVQRMEG
                     IIQHYLSHTRGSPRKRQINIKELVGETLLLLNPIFQQNDVQVTTDLAEPLPMLWGDDA
                     SLQRVLINLLDNAINASKEGGRLKIAARASNACEAKRPGVHIEITDNGAGIPAELLPR
                     IFELFVTTKPSGKGTGLGLVVCQEIVKAHGGNIDIKSEVGTGTSVRIYLPIDERSAES
                     DPSEGTE"
     gene            2138..>2830
                     /locus_tag="VFN58_06515"
     CDS             2138..>2830
                     /locus_tag="VFN58_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917678.1"
                     /translation="MNAHILIADDDEVSCQLFAETLESEGFQVEQTTSGEAALSRLRE
                     DTPDLLIIDVRMPGTSGLEVTRIVHEKYPSLPVIVMTAFGSIETAVEAIHEGAFDFIS
                     KPMNLAELKKTVSRALAQRALQRRAETNNGGDEENPQQLGKIIGKSPAMLEVYKTVAR
                     VAPTKSTALILGESGTGKELIAQAIHQHSPRANRPFVAVDCGALTETILESELFGHVR
                     GAFTGALADKKGV"
BASE COUNT          675 a          743 c          792 g          620 t
ORIGIN      
        1 agcagcgttg tcagcatctt gagaatcgtc gtcttgccgg ccccgtttgg accgagcaag
       61 ccaaaaactt cgcctcgctt caccgaaacc gtaagcgaat ctacggcagt atgctcgcca
      121 aagcggcgcg tcaaagacaa agtttccagt accggtgcat taactgctgc cggattgcct
      181 tccacgattg tgtgtccttt cccactatct tcttttagtg gcaatgcgat gacttctatc
      241 atccggtctc gtcaatagat gattcaggac ataggcaacg accagctgcc tctccttcgg
      301 tttaaattta aatagagctt cggtttcttc ccatatcgcc gagacaagct cacccaaagt
      361 tgtttttact cgaattcgcc tagaactttt catcgttcct ccataaaaat gaccacgact
      421 aacatttacc tacgcatgat gatttctccc ggggcaatct ttgtgccact cttgaaacaa
      481 atttcatcag cacgattgct tgtcagtggc tcacgcgatg cgcagcgcgg catgaaagaa
      541 cttcgtctgt gctttcaccg gcaagagagc tgaagatcgc gagtcacgag aaaagctttt
      601 cggccacagc ggttgagaca agaatgagcg cgcgcattac ctagatgagg caaaaatgcg
      661 tggtgggtgt ggcattgttg cagcggctaa ttaattgaag tagacgttga gcatactgag
      721 aatattgaga ttagtgccat tgctcccgat tggaatgaaa tttgtgaagg tggaagaaga
      781 agccacatcg agtcacgctg tggtggggag gtcgtactgg aacacactgc caaactcatc
      841 tgaagatctc ccgccgggcc tgaagattcg gacgtgtaca cgtgatttca atgcctgttt
      901 cagctacaac cgaacaaagc ttttccaact ggtgcaggca aatgttgatt gagagccgcc
      961 cccccatagt gcggtatggg attgccgtgg caagcgtggg gctggccctt gctgtagcgt
     1021 cgctattgcc gtatcgcgcc gatccgagtc acttcacact gttcttcgcc gctgtaatga
     1081 taagttcctg gtacggcggt cttggaaccg gcctagtggc caccatcctt tctgccttgt
     1141 ctctcgatta tttttttatc gccccgattc actctataac cttagactgg caggcgtttt
     1201 tgcggttagg cgtattctta gttgtagcag tcgtgaccag ctatctgact acggcgcgaa
     1261 agcgcgcgga agaggcgctg cgcaaagcgc acgcggagct agaagaacgg gtccgagaac
     1321 ggactgcaga gctgactcga gccaatgaat cgttgcgggc cgagattgtc gagcgcaagc
     1381 gagccgaaaa cgaactctgg ggtgtccagc aagagatggg gcgggtggaa aggcttgccg
     1441 ccctgggccg catgaccggc accattgccc acgagttggg cacgccgttg aactccgtgc
     1501 tcggctacac ccaactgcta gctcaggagg cgttgtcgga gggcgcgcgg cgccgcctca
     1561 acattattga gacccaggtg caacgcatgg aaggcatcat tcaacactac ctctctcaca
     1621 cgcgcggctc tccgcgtaag cgccaaatca atatcaaaga gctggtgggt gagaccctat
     1681 tgttgctcaa tccgattttc cagcagaacg atgtgcaggt gacgacggac ttggccgagc
     1741 cgctgcctat gctttggggc gacgacgcct cgctgcagcg ggtattgatc aatctactcg
     1801 acaacgcaat taacgcgagc aaggaaggcg gccgcttgaa gattgccgcc cgcgccagca
     1861 atgcctgcga ggctaaacgt ccaggggtcc atatagagat caccgacaat ggcgcaggca
     1921 taccggcgga gttgttgccg agaattttcg aacttttcgt aaccaccaaa ccgtccggga
     1981 aagggacggg tctgggactg gtggtctgcc aggaaatagt caaggctcac ggcggcaaca
     2041 tcgatattaa aagtgaagta ggaacgggca cgtccgtgcg gatttatctg ccaatcgatg
     2101 agagatccgc ggagtccgac ccaagcgagg gaaccgaatg aacgcgcata ttttgattgc
     2161 cgacgatgat gaagtgagtt gccagctttt tgccgaaaca ttggaatcgg aaggattcca
     2221 ggtggaacag acaacctcgg gggaggcggc gctttcacgc ttgcgtgaag atacccccga
     2281 cttgctgatc atcgacgttc gaatgccggg gacctcaggc ttggaagtaa cccgcatcgt
     2341 tcatgaaaaa tatccctcct tgccggtcat cgtgatgacc gcgtttggat ctattgaaac
     2401 cgccgtcgaa gcgattcatg aaggcgcatt tgacttcatc tcgaagccga tgaacctggc
     2461 agagttaaaa aagactgtgt cgcgcgcatt agctcagcgc gcgttgcagc gccgcgcgga
     2521 aacgaacaac gggggagatg aggagaaccc gcagcaactc ggcaagatca ttggcaagag
     2581 tccggcaatg ctggaagttt ataaaaccgt ggcgcgggtc gccccgacga aaagcaccgc
     2641 gctcatcctc ggcgaaagcg gcaccggtaa ggaactaatt gctcaggcga ttcaccaaca
     2701 cagtccgcga gccaaccgcc cctttgtcgc ggtggattgt ggagcattga ccgagacgat
     2761 tctcgaaagt gagctcttcg gccatgtgcg gggcgcgttt accggagcgc tggcagacaa
     2821 aaagggagtt
//
LOCUS       DASSWZ010000270         4126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1156365, whole genome shotgun sequence.
ACCESSION   DASSWZ010000270 DASSWZ010000000
VERSION     DASSWZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156365"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VFN58_06520"
     CDS             <1..1098
                     /locus_tag="VFN58_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917679.1"
                     /translation="RMTIATLFLVFFTIGLVGAAEWQSEWESALKGAKKEGKVVIAIP
                     PSAELRKELEPLLKQKFGIEAELVVSRGADSANRIASEFKAGVRYFDMIIQGTTTAMS
                     LQDGGMLDAVNSYMILPEVKDPKYWWGGHIWNDNIKTNRFLYSFVANAGTEGLFYNPE
                     LAKPDELRSFDNLLNPKWKGKIGLNDPRIGGSGISLWSFMWETKGEEYLRKLVQQDLF
                     LSRNLRQIADALAKGKLAITIGLGRAEYDPFMSAGLPVKELPRPKEGLPASSGYGVLG
                     IVKDPPHPNTTKVFVNWFLGKEGQEFYAKVMKQGTRRLDVDTKWMRAEGVDAAKDVMT
                     IAEYDRVRNHLEDKVINVRRPAAQFAAKILN"
     gene            complement(1144..2400)
                     /locus_tag="VFN58_06525"
     CDS             complement(1144..2400)
                     /locus_tag="VFN58_06525"
                     /inference="COORDINATES: protein motif:HMM:NF004788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET8917680.1"
                     /translation="MLAIMPAQVLGQPVEQVLSLAKKEKPVLLETLKELVSIESGSRD
                     NEGLDKLANLIASRLKSLGGQVALVEPTDIYKMEDTPEKIGKIVRATFAGAGTKKILL
                     IAHMDTVYPRGMLAQQPFRIEGDRAYGLGIADDKQGIAVILHTLAILKTMDFREYDTL
                     TVLINADEELSSPGSRSLITKLGAEHDATFSVESSRAESDKLSLATSGIASVTLTVQG
                     RASHSGVKPEAGVNAFYELAHQVLQTRDFSDPAVGLKMNWTMANAGTTRNMIPPQAQA
                     VADVRVLRVADYDAIEKKVREQIKKQLLPEAKVEINFERRRPPLEATVASQALAKHAQ
                     QIYRELGKELVVDDMPEGGGTDAAFAALNTKAPVIERFGLQGYGAHSADAEYVLLDSI
                     EPRLYLLARMIMDVSKGKGPQSASQK"
     gene            complement(2813..3583)
                     /locus_tag="VFN58_06530"
     CDS             complement(2813..3583)
                     /locus_tag="VFN58_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET8917681.1"
                     /translation="MNRSRRNLLLGAVQGAGLLMLSGCEKAFNSLQQNKKFLSLLESA
                     EGANQRLQRLLTGKNKLAQEFTEQDISRYFKPNGNPPPITMEYIADAKNNWQAWRLEI
                     AGLVKQPAQFSLAELRSMPSRTQITRHDCVEGWSAIAKWKGVPLTEIMQRVQPGSEAK
                     YIVFHCMDTDDSGAHYYESIDFRDANHPQTILAYEMNDRAVPVEHGAPLRLRIETQLG
                     YKHAKYIRRIEFVASYEKIAAGKGGYWEDQGYEWYAGI"
     gene            complement(3590..>4126)
                     /locus_tag="VFN58_06535"
     CDS             complement(3590..>4126)
                     /locus_tag="VFN58_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET8917682.1"
                     /translation="PLQKMAYTSVFLFLTPLIMLSGMAMSPQLDAAFHWLPAMFGGRQ
                     SARSIHFILTFLFVGFTFGHVFMVLATGILNNMRSIVTGWYKETGSDVPEPAWLQDSK
                     EEMIQKPSGVPLSHQLLVPELEEIPPAQETHQNHTDAPQPDNGISKSPPNDVQISPES
                     SKQTSPTHNNEVKKDIVE"
BASE COUNT          927 a         1038 c         1149 g         1012 t
ORIGIN      
        1 cggatgacta tagctacgct gtttttggtt ttcttcacga tcggcttggt gggagcggcc
       61 gagtggcaat ccgaatggga atcggcatta aaaggcgcaa aaaaagaagg caaggtcgtt
      121 atcgccatac ctcccagcgc cgagctgaga aaagagttag agcctttgtt aaagcaaaag
      181 ttcggaatcg aagccgagct ggtggtttct aggggagccg attccgcgaa tagaattgcc
      241 tctgaattta aagcgggagt gcgttatttc gatatgatca ttcaaggcac aaccaccgcg
      301 atgtcactcc aagacggcgg gatgttagac gctgtcaact cttacatgat tcttcctgag
      361 gtcaaagatc ccaaatattg gtggggcgga cacatttgga acgacaacat caagacgaac
      421 cggtttcttt attcctttgt cgccaatgca ggtacggagg gtcttttcta taacccagag
      481 ttggcgaagc ccgacgaatt gcgctctttt gacaatcttc tcaatcctaa atggaagggc
      541 aagatcgggt tgaatgatcc gcgcattggc gggtcgggca tatcgttgtg gtctttcatg
      601 tgggaaacca agggtgaaga atacttgcga aaactcgtcc agcaggatct ctttttaagt
      661 cgcaatctcc ggcagattgc cgacgctctt gcaaaaggca agctcgctat tactatcgga
      721 ctgggtcgcg ccgagtacga tccattcatg agcgcgggtt tgccggtcaa ggaactcccg
      781 agaccaaagg aaggcttgcc tgccagcagc ggctacggcg tccttgggat tgttaaggat
      841 cctcctcatc cgaacacgac caaggtcttt gtcaattggt ttctaggtaa agaaggccaa
      901 gagttttatg ccaaggtgat gaaacagggc actcgccgtc tggatgtcga caccaaatgg
      961 atgcgagcag aaggcgtgga tgccgctaaa gacgtgatga caatcgccga gtatgaccga
     1021 gtacgaaatc accttgaaga taaagttatc aatgtgcgca ggccggcggc acaattcgct
     1081 gcgaaaattt tgaattagtc acatacgctg tctctatctc aatcaagtga aatcgaccaa
     1141 tcattatttc tgagaagcac tctggggacc cttacccttt gacacgtcca taatcatacg
     1201 cgcgagtaga tacagacgcg gctcgattga gtcgaggaga acgtattccg catcggcgga
     1261 atgggcgccg taaccttgca ggccgaagcg ttcgatgacc ggcgccttgg tgttgagcgc
     1321 ggcaaaggcg gcatcggtgc cgccaccttc cggcatatcg tccacgacga gctctttgcc
     1381 gagctcacgg taaatctgct gagcgtgctt ggccagcgcc tgtgaggcta ctgtggcctc
     1441 cagcggtggg cggcgacgct cgaagttgat ttcgactttc gcctcgggca gtaactgttt
     1501 tttgatctgc tcccgtacct ttttttcgat ggcgtcgtag tcggcgacac gtagcacgcg
     1561 gacgtctgca accgcctggg cttggggcgg aatcatgttg cgtgtggtgc cggcgttcgc
     1621 catcgtccag ttcatcttga gcccgaccgc cggatctgaa aaatcacgcg tttgcagcac
     1681 ctggtgcgcg agctcgtaga aagcgttgac acctgcctct ggctttacgc cagaatgcga
     1741 cgcgcgccct tgtacggtga gcgtgacgga ggcgattcca ctggtcgcca gcgataattt
     1801 gtcggattcc gcgcgcgagg attcgactga gaaggttgcg tcgtgttcgg caccaagctt
     1861 ggtgatcaag ctgcgcgaac ccggcgagct gagctcctcg tcggcgttga tgagcacggt
     1921 gagggtgtca tattcgcgga aatccatcgt cttgagaatc gccaacgtat ggaggataac
     1981 ggcgatcccc tgcttgtcat ctgcgattcc caggccgtag gcacgatcgc cttcgattcg
     2041 gaagggttgc tgcgcgagca tgccgcgcgg gtacactgtg tccatgtgcg cgatgagcag
     2101 gatttttttc gtccccgcgc cggcaaaagt cgcccgcacg atcttgccga ttttctcagg
     2161 cgtgtcctcc atcttgtaga tgtcggtggg ctcgaccaac gcgacctggc cacccaaact
     2221 tttcaagcgg ctagcgatga gatttgcaag cttgtcgaga ccctcgttgt cacggctgcc
     2281 ggactcgatg gacaccagct cttttaaagt ttcgagtagg actggctttt ccttcttggc
     2341 tagggacaac acttgctcga caggctggcc aagtacctgg gccggcataa ttgccaatat
     2401 tagaaacaca atagccttga aataaccgaa gctctcgtca gatttcacag aatcacctcc
     2461 tgtaaaagcc gagaagatcg cgcaagaaga tcatccaaaa agttactccg aactacatcc
     2521 cgcgtttcgg gtaatcaaag aaatttgctc ttgagtttag ccaccgagaa ctcgagaatc
     2581 gcgcctacga ccgaacttta gccagtcttc gacagctcga agcggtaact agaggaagga
     2641 gactgcctcg ccagtcattc ggctggacag tatcgaataa cagaggtttg tgtcaactta
     2701 tggagtgatc cccataagtt tctttgggcc gttatcaggc tacgtgtgtg taggcggtta
     2761 tttagacaga ttcattgctg cttgatcaca gattgtcagg tgagtcaaaa attcagatgc
     2821 ccgcgtacca ttcataacct tgatcttccc agtagccgcc tttacccgcg gcgatcttct
     2881 cataactggc gacgaactcg atcctgcgga tgtatttggc gtgtttgtaa ccgagttgag
     2941 tctcgattcg aagccgcagt ggcgcgccat gttcgaccgg cacggcgcga tcgttcattt
     3001 cgtaagccag gatcgtctgg gggtggttgg catctcgaaa gtcgatactc tcatagtagt
     3061 gggcgccgct gtcatccgta tccatgcagt gaaagacgat atatttcgct tccgagcccg
     3121 gttgtactcg ctgcatgatt tctgttaaag gaacgccctt ccatttcgca attgcgctcc
     3181 agccttcgac acaatcatgg cgggtaattt gggttcgcga aggcatcgat cttagttctg
     3241 ccaaagaaaa ttgagccggc tgctttacta ggcctgcaat ttccaagcgc caggcttgcc
     3301 aattgttttt ggcatcggct atgtactcca tggtaattgg cggtggattg ccgttgggct
     3361 tgaagtaacg cgagatgtct tgctcggtga attcttgggc gagcttattc ttaccggtca
     3421 gtaatcgttg caggcgttga ttcgcgccct ccgccgattc cagcagcgaa aggaatttct
     3481 tgttttgctg aaggctgtta aaagcctttt cgcagccgga aagcatcaac aagcccgcgc
     3541 cttgaacggc gccgagaagc aaattgcgtc gtgatcgatt catagggact tactccacga
     3601 tatctttttt tacctcattg ttatgggtgg gagaggtttg tttagagctt tccggtgaaa
     3661 tttgcacgtc gttgggcggt gatttcgaga tgccgttgtc tggctgcgga gcgtcggtgt
     3721 gattctgatg cgtttcctgc gccgggggga tctcctcaag ctctggcacg agtagttggt
     3781 gcgataacgg aactccggag ggcttttgaa tcatttcctc tttggaatcc tgaagccatg
     3841 ccggttccgg cacgtcagaa cccgtttcct tataccagcc ggtaacaatc gagcgcatgt
     3901 tgttgaggat gccggtggcc agcaccatga agacatggcc gaaggtaaag ccaacgaaaa
     3961 gaaatgtgag gatgaagtga atgctgcgcg ctgattgacg cccgccgaac atcgccggca
     4021 gccaatggaa cgccgcatcc agctgcggtg acatggccat tcccgagagc atgatcagcg
     4081 gagtcaggaa taagaagacg ctggtatacg ccatcttttg tagcgg
//
LOCUS       DASSWZ010000271         4597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_831436, whole genome shotgun sequence.
ACCESSION   DASSWZ010000271 DASSWZ010000000
VERSION     DASSWZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831436"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFN58_06540"
     CDS             <1..380
                     /locus_tag="VFN58_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET8917683.1"
                     /translation="DGHDRGVKIVARALRDAGMEVIYLGIHNTPEEIASSALQEDVDA
                     IGLSIHSAAHMTLFGEVLGLLRKKKARDIAIFGGGIVPQEDIHSLKRMGVKEIFTPGA
                     PLEEIVNFVNSLPPRKRNASLKQ"
     gene            428..1597
                     /locus_tag="VFN58_06545"
     CDS             428..1597
                     /locus_tag="VFN58_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8917684.1"
                     /translation="MNFDLKEEQKLLKQTIRDFAETQIVPGAAARDEAARFPTELIPK
                     MAEVGLFGIMIPQEYGGAGLDTLSAAIIGEELARVDAAIALIVASHNSLCAAHILNFG
                     SEMQKQKYLPSLARGEKLGAWALTEPGSGSDAAALKTRATLEGEHWVLSGEKQFITQG
                     STAGVYVIMTSTEPSQGKRGISAFIAESGTAGLRVGKIENKLGVRASDTVAVQIEDAR
                     VPKANLLGQLNGSFDDVLKVLQGGRVGIGAMAVGIAQGAFEESIKYARMRKQFGKPIA
                     EFEAIQWMLADMATEIDAARLLVYRAAQLKERGMPFVTAASEAKLYAAETAMRSTTKA
                     IQIHGGYGYIKDYPVERYFRDAKICEIGEGTSEIQRMIIAKELLRGWSDGVQGME"
     gene            1665..2588
                     /gene="meaB"
                     /locus_tag="VFN58_06550"
     CDS             1665..2588
                     /gene="meaB"
                     /locus_tag="VFN58_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET8917685.1"
                     /translation="MTNNLVKRILNGDVRALASLMSLIEDGDPKVKAVLKAIYTHAGR
                     AHVIGVTGAAGTGKSTVIDRMTAEYRRRRKTVGILAVDPTSLFSTGAVLGDRVRMRDH
                     FVDDGVFIRSFATRGSSGGVSAAVRKAIHLLDAAGKEVIFVETIGVGQDQLEIAALAQ
                     MVVVVLTPQMGDEVQGMKAGLAEIADILVVNKADLSGADETVQQLTALFADEDFLILA
                     TSAIKDQGIDLLVDRIEEHRKKSLGNGKYRKKRLNLCRQELLSLLREKVFAELAKKID
                     NDSLEAQVKLIAERQIDPYSAADRLARRIDL"
     gene            complement(2736..3662)
                     /locus_tag="VFN58_06555"
     CDS             complement(2736..3662)
                     /locus_tag="VFN58_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET8917686.1"
                     /translation="MNSGIRKVGVIGIGLMGSGIAQVAAAKGFATLVVDVTPEIVGRG
                     MERIRNSLHRLVESYQKSSGETGVAPEEEANIVGRIKTSTERHDLLDCDILIEAVVEE
                     EAAKRTLISSLSEMGYRKLFVSNTSSISITRLASSYPLPEKFMGMHFMNPVPIQPGCE
                     LIRGFLTSQETFQKVLEFCRELGKEPILAEDKAGFGINRMFVPFLNEAVKVVEEGIMS
                     CDDADKTTLCLGHKMGPITTLDYVGLDTTLSIAQVLERELGPGYKPPDLLCKLVAAGF
                     YGIKNGRGFYLWEQGKKVGVNPAVQRYRRGPN"
     gene            complement(3929..4414)
                     /locus_tag="VFN58_06560"
     CDS             complement(3929..4414)
                     /locus_tag="VFN58_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET8917687.1"
                     /translation="MKGTEMMQLPTNIAQRAWPGKIPLTSLYTAGTGGQIFFHALKTQ
                     GKLIGTWCSPCDQVYLPARSFCERCFAELTEQVEVKRTGRLVSYTLSYVDHDGARLRR
                     PLALGLVQLEGATTLILHRLLGVNGPTEVKIGCRVETLIKPKAKRIGSILDIEGFRVV
                     S"
     gene            complement(4411..>4597)
                     /locus_tag="VFN58_06565"
     CDS             complement(4411..>4597)
                     /locus_tag="VFN58_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET8917688.1"
                     /translation="PEIPAVIEIDGASRGMGILHVVKKIAPKKVRIGMAVRAVWKPKS
                     RRIGSVTDIAYWEPIKK"
BASE COUNT         1075 a         1204 c         1310 g         1008 t
ORIGIN      
        1 tcgacggtca cgaccgcggg gttaagatcg tcgcacgggc gctgcgggat gccggcatgg
       61 aggtgatcta tctcggcatc cataacactc cggaagagat cgccagctcg gcgcttcaag
      121 aagacgtgga cgctatcggt ctttccatcc actccgccgc tcatatgact ctgttcggcg
      181 aggtgctcgg gctcctcagg aaaaaaaagg cgcgggatat cgcaatattt ggcggaggaa
      241 tcgttcctca ggaggacatc catagcctca agaggatggg agtcaaggag atctttacac
      301 cgggggcgcc ccttgaagag atcgttaatt ttgtaaactc gctgccgcca agaaaaagaa
      361 atgcgtcgct aaagcaatga attcgcttcc aaatgttggc caagagttga agtgaacgaa
      421 gagcgttatg aattttgatc tgaaggagga gcaaaaactc ctcaagcaga cgatccgcga
      481 ttttgccgag acgcagattg tgccgggagc ggcagcgcgc gacgaggcgg cgcgctttcc
      541 gacggagctg attcccaaga tggccgaggt gggcctcttt ggcatcatga ttccgcagga
      601 atacggcggg gctggtttag ataccctcag cgccgcgatc atcggcgagg agctcgctcg
      661 tgtggacgcc gcgatagcgt tgattgtcgc ctctcataat tcgctctgtg cggctcacat
      721 tcttaacttt ggtagcgaaa tgcagaagca aaaatacctg ccttctttag cgcgtggcga
      781 gaagctcggt gcctgggcac tgaccgagcc cggatctggc tccgatgccg ccgctcttaa
      841 gacccgagca acgctcgaag gagagcattg ggtgctcagc ggggagaaac agttcatcac
      901 ccagggttcc accgcgggcg tctacgtgat tatgacgtca acggaaccgt ctcaaggcaa
      961 gaggggcatc tcggcattta ttgctgaaag tggaacagcg ggactgcgag tcggcaagat
     1021 tgaaaacaag ctcggggtga gggcttctga tacggtcgcc gtgcagattg aggacgcacg
     1081 ggttccaaaa gcaaatctct taggccaact caacggcagc ttcgacgatg tattgaaggt
     1141 tttgcagggt gggcgagtcg gcatcggggc gatggcggtg ggaatcgcac aaggcgcttt
     1201 cgaggagtcg atcaagtacg ccaggatgag gaagcagttt ggcaagccga ttgcggaatt
     1261 tgaggctata cagtggatgc tggctgacat ggccacggaa atcgacgccg cgcgcctttt
     1321 ggtctatcgt gccgcgcaac tcaaggaaag aggaatgccc tttgtaacgg cggcctcaga
     1381 ggctaaactc tatgctgccg agacggccat gcggtcgact acgaaagcaa ttcaaatcca
     1441 tggaggttac gggtacatca aagattatcc tgtggagcga tactttcgtg atgccaagat
     1501 ctgtgagatt ggcgagggga cctcagagat tcagcggatg ataatcgcga aggagttatt
     1561 gcgtggttgg agcgatggcg tacaaggaat ggaatgatgg atagtggagt attgataaag
     1621 aatatccgtt actccagtac tttactgttc gtctcatccg gccaatgacg aacaatctcg
     1681 taaagcggat tctcaacggt gatgtccgcg cgctggcatc gcttatgagt ctgatcgaag
     1741 acggagatcc gaaggtaaag gccgttctca aagccattta tacacatgcg ggaagagcgc
     1801 atgttatcgg agtgaccggc gcggctggaa ccggcaaaag caccgtcatc gatcgcatga
     1861 ctgctgagta tcggcggcgg aggaagacgg tcggaattct cgcagtggac cccactagcc
     1921 ttttctcgac cggggccgtt ttgggcgacc gcgttcggat gcgcgaccat tttgtcgacg
     1981 atggtgtttt catccggagc ttcgccaccc gaggaagcag tggcggcgtg tcggctgctg
     2041 ttcgaaaagc catccatctg ctcgatgccg cgggaaaaga agtgatcttc gtcgagacca
     2101 taggggtcgg gcaagatcag ctagagatcg ccgccctagc tcagatggtc gtggtcgtgc
     2161 tgacgccaca gatgggagac gaagttcaag gtatgaaggc tgggctggcc gaaatcgccg
     2221 atattctggt ggtcaacaaa gccgatcttt ccggcgcaga cgaaacggtt caacaactca
     2281 cggccttgtt tgccgatgaa gattttctca tcctggctac ttccgcgatt aaggatcagg
     2341 gaattgattt gctcgtggac cgcatcgaag agcaccggaa gaaaagtctc ggcaacggca
     2401 aatatcgaaa aaaacgactc aatttgtgcc gacaggaatt actttctctg ttgcgcgaga
     2461 aagttttcgc tgagcttgca aaaaagattg acaatgattc gcttgaagcg caggtcaagc
     2521 tgatcgccga acggcaaata gatccctata gcgccgcaga ccggctggca agacgtatcg
     2581 acctatagag gcgaaggccc acggaacggt ttgagtttag cggccgtgaa attcgtcagc
     2641 gatttaccca aacgcgcaat gccaaggcat gcgatgcgtg attcgcgctc gttattgtca
     2701 aaatcgaagg atccaaaggg cctttgcccg cgccgctagt tcggtcctcg ccggtaacgt
     2761 tgcacggcgg gattgacgcc gaccttcttg ccttgctccc ataagtagaa accccggcca
     2821 tttttgatac catagaaccc tgcggcgaca agtttgcaca gcaaatccgg tggcttgtaa
     2881 ccgggaccca attccctctc taggacctgg gcaatggaga gagtggtatc gagacccacg
     2941 tagtcaagcg tggtgatggg ccccatcttg tggccgaggc agagggtggt cttatccgca
     3001 tcgtcgcaac tcatgatacc ttcctcgacc actttcaccg cctcgttcaa aaaaggtacg
     3061 aacatccggt tgatcccgaa tcctgccttg tcctcggcca ggatcggctc tttgccgagt
     3121 tcgcggcaga actcgagcac tttctgaaat gtctcctggg aggtgagaaa gccccggatc
     3181 agctcgcacc ccggctgaat cggaaccgga ttcataaaat gcatccccat gaatttctcc
     3241 ggcaacggat aagaactggc gagccgcgtg atggaaatcg aggaggtatt ggaaacgaat
     3301 agcttccgat aacccatctc agaaagagac gaaatgagag tcctttttgc cgcttcctct
     3361 tccacaaccg cttcgatgag aatatcgcaa tccagcagat catgccgctc cgttgaagtc
     3421 ttgatacgcc cgacaatgtt cgcctcttcc tccggcgcga cccctgtctc gccactcgat
     3481 ttttgatagc tctccaccag gcgatgcagg ctgttccgga tacgttccat accccggccc
     3541 acaatctccg gagtgacatc gacgaccaac gtagcgaaac cctttgcggc cgcaacctgc
     3601 gcgatgccgc tgcccatgag accgatgcct atgacaccga ctttacggat ccccgaattc
     3661 atcggacaac ctcctctcta agtattttta tcccgcggca gaagtttaca ctactggtct
     3721 gaaagaaaga atcataactt gttgttcgga ttagaacctt ctagctgtaa accgtctcct
     3781 tcagataaaa taatgtcggg aggggacgct atggtctggg cagcgcgagc atcggcggtg
     3841 gccagcgcat tgcgctgctt ttggaaagaa tctgacgttt ccttcgcaat tgtgcttggc
     3901 gctgctgtga ctaagcaacg agtgggattc aagaaaccac acgaaagcct tcaatatcga
     3961 ggatggagcc gatacgcttc gctttcggtt tgatgagagt ctcgactctg cagccgattt
     4021 tcacctccgt ggggccatta acgcccagca gacggtgaag aatcagtgtg gtggcacctt
     4081 ccagctggac aagccccaga gctaagggtc ggcgcagccg tgcgccgtca tgatcgacgt
     4141 aagacagggt gtagctaacc agtcggccgg ttcgtttgac ttcgacctgt tctgtaagct
     4201 cggcaaaaca gcgctcgcag aacgagcggg cgggtagata aacctggtcg caagggctgc
     4261 accatgtacc gatcagtttg ccttgcgttt taagtgcgtg aaagaaaatc tgcccaccgg
     4321 ttcctgccgt gtagagcgaa gtgagtggaa tttttcctgg ccaggcgcgc tgcgcgatgt
     4381 ttgttggcaa ctgcatcatc tccgtacctt tcacttttta atcggctccc agtaggcgat
     4441 gtccgtgacg ctgccgatcc gcctcgactt gggcttccaa accgcccgga ccgccattcc
     4501 gatgcggact tttttcggcg caatcttttt gacgacgtgc aagattccca taccgcgcga
     4561 agccccgtca atttcaatca cggcagggat ctccggc
//
LOCUS       DASSWZ010000272        10026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_24858, whole genome shotgun sequence.
ACCESSION   DASSWZ010000272 DASSWZ010000000
VERSION     DASSWZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24858"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1338..1414)
                     /locus_tag="VFN58_06570"
     tRNA            complement(1338..1414)
                     /locus_tag="VFN58_06570"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1378..1380),aa:Met,seq:cat)
     gene            complement(1475..2179)
                     /locus_tag="VFN58_06575"
     CDS             complement(1475..2179)
                     /locus_tag="VFN58_06575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET8917689.1"
                     /translation="MRCYVIGDIHGCLDELRYLVEGLPLESGDRLVFLGDYVDRGPNS
                     KGVLTYVLQLQQRDDLELICLKGNHEDMFLSYLGLPGHHGDMFLYNGGYATLISYGVS
                     SKQSSLDEITSQIPPDHIEFLKNLRMYFVMEPFICVHAGINPSKPLAKQTDTDLLWIR
                     DEFIHNPHRLPYTVIFGHTPRNAVLFDLPYKIGLDTGLVYGNKLSCLEIVEKVLYQIT
                     RGQKDIKRIVALDQWN"
     gene            2478..3308
                     /locus_tag="VFN58_06580"
     CDS             2478..3308
                     /locus_tag="VFN58_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917690.1"
                     /translation="MLKPIGLIEGHYECRSLDETLPIFTDLLALEVVERKDKQAVVKH
                     PNTEWRLVVHEGGPNAANKPHTNHYGFRVGSSKEIPKAYEYLSDHKTQYKIKSVTKPH
                     EAHFAYSVYFKEPGGNDLEIEYYNPAAIKHGRSIAAPHWKEPLSEEQFPGRGYIPQAM
                     THGTLNCDDKATNDHFLEEVLGLKIVGGGRMSTYIKHPDTPWYIVVLPTTHRDYLTSA
                     NRFTLKVATPEEVEDAHKEFSTNGKQVGVNELWDLEEENGRAFFIFSDVSKNWWEVTS
                     "
     gene            complement(3437..4426)
                     /locus_tag="VFN58_06585"
     CDS             complement(3437..4426)
                     /locus_tag="VFN58_06585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8917691.1"
                     /translation="MRFSLHSVHGGVRNIDEIHTRAQRVEALGYNGIFLGESHLSSID
                     SFQALATCAMLTKRVLLGIAVTNMVFRHPTVLAGAAASLNEISKGRAILGLGTGDGPV
                     YSQGRKATPLDEFETGVKMIRDLVQGKPMTFPKGEVGIAFNLHPVPIYVSAEGPKGLR
                     LGGRCADGVILGTGFDLRVLDWARQRICEGIREAGRQDSEVALIAAGMLCVRDDGHEA
                     RRIVRNRIANRAHHNFRFTLETVPPEQLEGIRKFMAGFDVMKPMEDRVDPDLVNDYLV
                     HRFSIAGTPEECIKRIEELRDAGVEHLMLTPARKVYHESVETFAAKVIPHFRD"
     gene            complement(4461..5279)
                     /locus_tag="VFN58_06590"
     CDS             complement(4461..5279)
                     /locus_tag="VFN58_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HET8917692.1"
                     /translation="MPIVFACAASHAPGMTAWTEAAPKEQSEKFLGCYRKLGEMLAAS
                     RPDVLVAITVEHWANFFLNHMPAFCIGRADHYEGPVEEWLRIPKARVPGDSKLSAELL
                     AACLDGGFDPTFSDELLFDHATMLPLHFLNPNMAVPVIPVIINTLTTPMPTPKRCFAF
                     GQFLGKNLAGCEKRVAVVATGGLSHWPGEAKHGKINIPFDRKFLDTLISGDRAKLSAY
                     SHEEINLEAGSGGHEIRTWIALAGALPEWRAELLAYEPVVPWATGCGLVVFSRS"
     gene            complement(5283..5537)
                     /locus_tag="VFN58_06595"
     CDS             complement(5283..5537)
                     /locus_tag="VFN58_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917693.1"
                     /translation="MSLYQLQKLIYQVNRDPSQRERYRQDPAEFVKGYELTEEEKNAA
                     LNVDVRKLYTFGVHSLLLRPFTLLHKVSNEDYAKALAGLE"
     gene            complement(5540..6634)
                     /locus_tag="VFN58_06600"
     CDS             complement(5540..6634)
                     /locus_tag="VFN58_06600"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET8917694.1"
                     /translation="MDKPRTLEEIKCDIRSRVGHRAPFNHADKQEAEEVLARLNSVDG
                     EAWAAVWNALGARWEERARTAEAAGKRDEAKQAYLKSYGYYGIGRHPFPSTPGKQHAY
                     LKAREMHLAASRYFDIPLERLAIPFNGKEIIGHLRLPKKTPAPLVMHWGGIDNWKEER
                     HTFAERFVKEGWGCLVLDSPGTGECPMLASPDAHQVHVAALDYMLKRREIEPDRIAVV
                     GASFGGYWATKLAHVEPKRLRAVVNWGGGIHSFFQPEWQERSRNADSYLFDLIEARAN
                     LFGKKTMDELNQVMPALSLLAQGWLDRPSAPMLLVNGKEDKQVPLEDFYLLLEHGHPK
                     TIRLFPGGHMGNIPFVFETVIPWLHQKLDN"
     gene            6957..7817
                     /gene="folD"
                     /locus_tag="VFN58_06605"
     CDS             6957..7817
                     /gene="folD"
                     /locus_tag="VFN58_06605"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET8917695.1"
                     /translation="MASIIDGKAVAKEVQKQIKEEVEGLERRWGLVPGLGVVLVGDDP
                     GSHIYVKNKEKACKEVGIKSYEHLLPATVSERELLALVHSLNKDKNVHGILVQLPLPA
                     HIRSEKILEAVSPHKDVDGFHPVSQGMLLLGGDGFKPCTPMGIMKLLESVGCDPKGKN
                     AVVVGRSNIVGKPVALMLLERHATVTICHSRTASLRDEVARADILVVAIGKAGLVRGD
                     WVKPGAVVIDVGVNRLTSGKLSGDVEFETAKERAAWITPVPGGVGPMTICMLLYNTLK
                     AAKDSLQRER"
     gene            7860..8939
                     /gene="gcvT"
                     /locus_tag="VFN58_06610"
     CDS             7860..8939
                     /gene="gcvT"
                     /locus_tag="VFN58_06610"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET8917696.1"
                     /translation="MKRTPLYGAHRRAGAKMVEFAGWEMPVQYGGVIDEHVAVRTRAG
                     LFDVSHMGEIVIRGPGTLELCQKISANDVSRMKLQQAQYNLLMNDKGGIVDDVIFYKL
                     EANHFLICVNASNSDKDFAWIEAHAEGNVELENASSRYAQLALQGPLAEKILQPLTSL
                     RLGEIRPFFFAFGDVSSIRCLVARTGYTGEDGFELYCGSPEAETLWNALLESGGPVGL
                     EPAGLGARDTLRLEKAYPLYGHELDDTTTPLEAGLEWVTKLAKGPFVGREVLLKQKEE
                     GIRRKLVGMELLEPGIARAEYALFKDGKPIGRVTSGTKSPTLAKAIALAYVTIEESVP
                     DNTVEVEIRGRKTRAKIVPLPFYRR"
     gene            8977..9417
                     /gene="gcvH"
                     /locus_tag="VFN58_06615"
     CDS             8977..9417
                     /gene="gcvH"
                     /locus_tag="VFN58_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET8917697.1"
                     /translation="MEFPEGLKYSKEHEWVLVEGNTATIGITEFAQEELGDIVYVELP
                     EVGEKVVKDDPFGAVESVKAVSDVYAPVGGTVLEVNDVLPDNPETINDDPYGDGWMIR
                     VEMTDEEDLKDLMTAEEYAEYVEQQKEDEDEEEEDDEEEDEEKE"
     gene            9427..>10026
                     /locus_tag="VFN58_06620"
     CDS             9427..>10026
                     /locus_tag="VFN58_06620"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HET8917698.1"
                     /translation="MRYTPHTSSDQERMLRTIGIDSIEELYRHIPETLRNRAKIDLPG
                     GLTELAVRRRLAELASSNATASEWSFFLGGGIYHHFIPSAVDAVISRSEFSTSYTPYQ
                     PEVSQGTLQALFEYQTLICQLTGMEISNAGVYDGASAAAEAVLMSRRIQPMNRRRVLM
                     SRALHPHYRAVIATYLRNLDDMQLDEVPFDGSGATDVAEL"
BASE COUNT         2473 a         2483 c         2874 g         2196 t
ORIGIN      
        1 cgctaagacg gctaaagttg ccgcgggtaa agctcggaaa gcagtacagg tataggccgg
       61 cggatattga cgggtttata aaccttcatc tcgagtatcc ggaaatcggg aggcccaatg
      121 tcagtagaat acagaaaaaa tcgaaaacgg tggggctatc ggttctacct tcacgggcaa
      181 tgcttcagca aatacgtctg ggacaccaaa acggaggcaa agcaggcgga acaggaagcg
      241 caagttgagg cgagaaagaa gccgggacta cagccaacgg cgctactcac agcaagcggt
      301 gcttatctga tagcgtccgc ggaacgaggt agatcgaagt ggcggataga cggactcaac
      361 tacacgttca aggcgcatat catcccgcac tttggcgagg ctgccttgat aaccgacatc
      421 accccgcaaa acgtcgagaa cttcatcgcg gcgctgaaac gaaaaggact caagaacaag
      481 accgtcaaga acatcatcac ggatctccgc gcgctgttta actgggcgat ggaagaagac
      541 atcaacctta tgacaagaaa cccggtaacc aagaaggtgg ccaagctcat cggtaatacg
      601 agagcggtaa aagccccgat caatccaagg tggttcgaca tcgcagcagc caacatagag
      661 aacaagcggg acaaggcttg gtttgacgtg acccgctttc tcgggatgag aaaagacgaa
      721 tcgaataggc ttcaatggat tgacgtcaac tggaacgcgg gcaaagtgag aataccagga
      781 acaaagaccg atgaagctga ggtgtggctg ccggttgcgc cagtggcatt gaaaacgctt
      841 agggatttat acgagtcaaa agatagggat ctcaactcgc cttacgtgtt tcccggcagg
      901 tcggcgcaga gcaagggaaa aaagatttac agcaggcgca ggatgtttga gagaatccaa
      961 caagtgacgg ctatcaagaa atacttacgg cagcatcccg gactcagcta tcagcaagcc
     1021 ctagaggcga cgcgcaaaga aggtttcaag ggcgggattc atctagcgcc taaggacttg
     1081 agagatttct tctgtactga gatcgcggct aagtcagatg atgccaatgt ggccatgagg
     1141 ctgatgaggc atacaagttt ggcgactacg actaaataca tgcgcacggt ggaagacagg
     1201 atgagagaag cggttgaaaa tcttggatgc gattcaatgg ccgcaactgg ccatgaaatg
     1261 tcacaactgg ccaacttgga agaactccaa aaagtagcca aaaagttgat aaaccaagga
     1321 tttctcaagg ggaaatttgg tggcggtgag tggagtcgaa ccaccgacgc cgcggatatg
     1381 agccgcgtgc tctgaccaac tgagctacac cgccacgtgg caatgtaaag tatgacgggt
     1441 tgaaaaaaag atcaagcttc ggaaatggac gaatctagtt ccactgatct aaagccacga
     1501 ttcgtttgat atccttctgg ccgcgggtga tttgataaag cactttctca acgatctcca
     1561 aacagctaag tttgttgccg taaacaaggc cggtgtcgag cccgatctta taggggagat
     1621 caaagagcac tgcgttccgc ggagtgtgcc cgaaaattac cgtgtaaggg agccggtgag
     1681 gattgtgaat aaactcatct cgaatccaga gaagatcggt atcagtttgt tttgccagcg
     1741 gcttcgacgg gttaatgccg gcgtggacgc agatgaatgg ctccatgacg aagtacattc
     1801 tcaagttttt aagaaattcg atatgatccg ggggaatctg tgaagttatc tcatctaaag
     1861 acgattgttt tgagcttact ccatagctga ttaaagtcgc atacccgccg ttgtataaaa
     1921 acatgtcacc gtgatgccct ggcagaccta gataagacaa aaacatgtcc tcgtgattac
     1981 cttttaagca gattagctcc agatcatcac gctgctgcaa ttgcagtaca tacgtcaata
     2041 cgcccttcga gttcgggccc cggtcgacat agtcgcccaa gaaaaccagc cggtcgccgg
     2101 attcaagagg aagaccctcg accaagtagc gcaactcgtc aaggcatcca tggatatctc
     2161 caataacgta acaacgcatg aagtgaccct atggtaagct gccaagcgaa cgcacgcaag
     2221 cgatggcgag ccgacgcgct acccggtctc ccgatcgtcg gtcaaaaatt caaactacgg
     2281 ccgggtaaga ttccatccgg catcgctcta gcatagagcg gaaggcagga tttttgagaa
     2341 agaatgcgaa atccggtgcg gcggtacgca tgccggcggt gggcccgcgg gcaattgaca
     2401 agagtcggag ggtttcttaa taataacaag agtagagtag cgcttaaaac gttggattct
     2461 acttgaggag taaaccaatg ctgaaaccga ttggactgat tgaaggacat tacgaatgcc
     2521 gttcgctcga tgaaacgctg ccgatcttta ctgatctttt ggctttagaa gttgtcgaac
     2581 gcaaagacaa acaggcggtc gttaaacatc ccaacacgga gtggcgcttg gttgtgcatg
     2641 aaggaggccc gaacgcggcc aacaaaccgc acactaatca ctatggcttc cgcgtgggga
     2701 gctccaaaga gatccccaaa gcgtacgaat atctttccga tcacaaaact cagtataaga
     2761 ttaagagtgt caccaagccc cacgaagccc actttgctta ctcggtttac ttcaaggaac
     2821 caggcggcaa tgacctcgaa atcgaatact ataatccggc cgcgataaag cacggccgat
     2881 ccatcgccgc cccgcactgg aaagaaccgc ttagtgaaga acagtttccg ggtcgtggct
     2941 atattccgca agccatgact cacggaactc taaattgcga cgataaagcg acgaacgatc
     3001 atttcctcga agaggtgctc gggctgaaga tcgtcggcgg cggacgcatg tccacttata
     3061 ttaagcatcc tgatacccct tggtacattg tcgtgttgcc gacgacgcac cgcgactatt
     3121 tgacgtcggc gaatcgtttc acgttgaagg tggcaacccc ggaggaagtt gaagacgcgc
     3181 ataaagagtt ttcgacgaac ggcaagcaag tcggggtcaa cgagttatgg gatctggagg
     3241 aggagaacgg gcgggcgttc tttatcttca gcgatgtcag caagaattgg tgggaagtga
     3301 cgtcctaaat tgcggccctt caaaaatcag cggaaacaac caaaggccgc gggcgtaaac
     3361 gccagcggcc tttgacgttt tgagctcgag cgccgcctga tgcgtacgtt ccgtgatctt
     3421 acggtgtcac aatatttcaa tctctgaagt gcggtatcac cttcgcggca aacgtctcga
     3481 cgctttcgtg ataaactttg cgcgccggtg tgagcatgag atgttcgacc ccggcgtcgc
     3541 ggagttcttc gattctcttg atgcattctt cgggtgtgcc ggcgatagag aagcggtgaa
     3601 cgaggtaatc gttaacgaga tcgggatcga ccctgtcttc catcggcttc ataacgtcaa
     3661 agccggccat gaatttccgt attccttcca attgttccgg gggcacggtt tctaaggtaa
     3721 agcgaaaatt atggtgcgcc cggttggcaa tccgattgcg gacgatgcgg cgcgcttcat
     3781 ggccgtcgtc gcggacgcag agcatgccgg cagcgatgag agcaacttca ctgtcctgac
     3841 gacccgcctc gcggatgccc tcgcagattc tctggcgcgc ccaatcaaga acgcgcagat
     3901 cgaagcccgt tccgagtatc acgccatccg cgcagcggcc tccgagccgc aggcctttgg
     3961 gtccttctgc ggaaacgtaa atcggcactg gatggagatt gaaggcaatc ccaacttcac
     4021 ccttgggaaa agtcataggc tttccctgaa ccagatcgcg aatcattttg acgccggtct
     4081 cgaattcatc aagcggcgtt gctttccgtc cttgcgagta taccggtcca tctcccgtgc
     4141 ccaatcccaa tatggcccgg cctttagaga tttcattgag cgaggccgct gcgccggcga
     4201 ggaccgtagg gtgacggaat accatgttgg tgacggcgat gcccagaagc acccgttttg
     4261 tcagcatcgc acaggtggcg agggcctgga atgaatcgat ggaactgaga tggctttcgc
     4321 ctaggaaaat gccgttgtaa ccgagtgctt ccacgcgctg cgcgcgtgta tgtatttcat
     4381 cgatgtttcg gacgccaccg tgaacgctgt gaaggctaaa acgcatgccg cgacttccaa
     4441 aaagcggatg cctcctggga ttaggaacgg ctgaagacaa ccaagccgca gcctgttgcc
     4501 cagggcacga ctggctcgta ggcgagcagt tccgctctcc attcggggag cgctcccgcg
     4561 agggcgatcc atgtcctgat ttcgtggccc ccggaaccgg cttcgagatt gatctcttca
     4621 tgggagtaag cagataactt cgctcggtcg ccggagatca aggtgtcgag aaactttctg
     4681 tcgaaaggaa tgttgatctt tccatgcttg gcttcgcccg gccaatggga caggccgccg
     4741 gtcgcaacga cggcgactct tttttcgcat cccgccaggt tttttcccag aaactgccca
     4801 aaggcgaagc atcgtttcgg tgtcggcatc ggtgtggtca aggtgttgat gatgacgggt
     4861 atcacgggaa cagccatgtt gggattgaga aaatgcaacg gcaacatggt ggcgtggtcg
     4921 aagaggagtt cgtcggagaa cgttggatca aaaccgccat cgagacaggc agcgagtaat
     4981 tcagcgctca attttgaatc gccggggacg cgcgctttgg gaattctcag ccactcttcc
     5041 actgggccct catagtggtc cgcgcgaccg atgcagaagg ccggcatatg gttcagaaag
     5101 aaattcgccc agtgctcgac agtgatcgcg actaggacat cgggcctaga ggcggcaagc
     5161 atctcgccca gctttcggta acagccaaga aatttttcag attgttcctt gggagcggct
     5221 tcggtccacg cggtcatgcc cggagcatga ctcgcggcgc aggcaaaaac gatcggcata
     5281 gtttactcca atcctgccag agccttggcg taatcctcgt tggaaacttt gtgtaacagg
     5341 gtgaagggcc gaagcagtag agaatgaacc ccaaaagtgt agagttttcg cacatcaacg
     5401 ttaagggcgg cgtttttctc ctcttctgtg agttcatagc ctttgacgaa ctcggcggga
     5461 tcctgtcggt atcgttcacg ttgagacgga tcccggttca cctgataaat gagtttctgc
     5521 agttgataga ggctcatatt cagttatcta gtttttgatg cagccacggg atgaccgttt
     5581 caaagacaaa tggaatattt cccatgtggc ctcccggaaa gagacggatc gtcttgggat
     5641 ggccatgctc cagcaaaagg taaaaatctt ccagcggcac ttgtttgtcc tctttgccgt
     5701 tgaccaatag catgggcgcg ctgggtctat ccaaccaacc ttgagccaac agagaaaggg
     5761 cgggcatcac ttgattcaac tcgtccatcg tcttcttgcc gaacaggttg gcccgcgcct
     5821 cgatgagatc aaacaagtaa gagtcagcat tgcgcgagcg ctcttgccac tccggttgga
     5881 agaaggagtg aatgccgccg ccccaattga ccacggcgcg cagacgtttc ggttcgacat
     5941 gggccaactt ggtggcccaa taaccgccga agctggcgcc gaccaccgcg attcggtccg
     6001 gttcgatttc gcgccgcttc agcatatagt cgagagcggc gacatgaacc tgatgggcat
     6061 cgggtgatgc aagcatggga cactcacctg tccctgggct gtcgagcacc aagcaacccc
     6121 atccttcttt gacaaaccgt tccgcgaagg tgtggcgctc ttctttccaa ttgtcgatgc
     6181 caccccagtg catgacgagc ggcgcgggag ttttcttcgg caagcggaga tggccgatga
     6241 tctctttgcc attgaaggga atggcaaggc gttcaagcgg gatatcgaaa tagcgcgatg
     6301 cggcgagatg catctcgcgg gctttcaagt aggcgtgctg tttgccggga gtgctgggaa
     6361 acgggtgacg gcctatgccg taatagccgt acgacttgag ataagcttgt ttcgcttcat
     6421 cgcgcttgcc ggcggcttcg gccgtgcggg ctctttcttc ccaacgtgct cccaacgcgt
     6481 tccagaccgc ggcccaagcc tcaccgtcca cgctgttcaa ccttgcaaga acttcctcgg
     6541 cttcttgttt gtcggcatga ttgaacggtg ctcgatggcc cactcggcta cgaatatcgc
     6601 atttgatttc ttccaaggtt cgtggcttgt ccatggcgct tcctattcca actatggcca
     6661 ttgaaatttc tcattttctt atcaaaaccg gacggagaaa gatacctccc atgctcggtc
     6721 atttcgcctt gagcctggtc gtaaataatt caggtgaacg cggtcgcaaa cttgtttttt
     6781 tattgcaaga ttctaaagtc ttagttagag gaatttattg attgcgttca ttgattcaac
     6841 gacgttgctc aacagacttt tgggggttga cctttggatg gctccatgtc atgccaatag
     6901 aaatgtccca tctttacaaa cttttttttt tcggtatagg tttttcggag taaatcatgg
     6961 catcgatcat agacggtaaa gctgtcgcta aagaagtgca gaagcaaatc aaagaagagg
     7021 tggaggggct ggagcggcgt tgggggttgg tccccggact gggagtggtg ttggtcggag
     7081 atgatccggg gtctcacatc tatgtgaaga acaaggaaaa agcatgcaaa gaagtaggga
     7141 taaaatctta cgaacatttg cttcccgcga cggtatccga gagggagttg ttggcgctgg
     7201 ttcacagtct caacaaggat aaaaatgtcc acggaatcct ggtccaactt cctttgcccg
     7261 ctcatattcg gtcagagaag attctcgaag cggtttctcc gcacaaagac gtggatggtt
     7321 tccatcccgt cagccaggga atgctgctgc tcggcggtga cggcttcaag ccctgcaccc
     7381 ctatgggaat catgaagctc ttggaatcag ttggttgcga tccgaaaggg aagaacgctg
     7441 tggttgtcgg ccgcagcaat atcgtaggca agccggtggc gctcatgctg ctcgaacggc
     7501 acgcaacggt gacgatttgt cattcgcgga ccgcaagctt gcgggacgag gtggcgcgcg
     7561 ccgatattct cgtggttgcc ataggcaagg cgggtctagt tcggggcgat tgggtcaagc
     7621 cgggtgccgt tgtgatcgat gtgggagtga atcgcctcac cagcggaaag ttgtccggtg
     7681 atgttgaatt cgaaacggcc aaggagcggg ccgcatggat tacgccggtc ccaggcggcg
     7741 tcggtccgat gacgatctgc atgcttctct acaacacgct caaagcagcc aaggactctc
     7801 tccagcgcga aaggtagaac ggtggttcgt cgggatttga gaacagcgtg gcgcaaggaa
     7861 tgaaaaggac tccgctctac ggcgcgcatc ggcgagcggg agcgaagatg gtggagtttg
     7921 cgggctggga gatgccggtt caatatggcg gtgtcattga cgaacacgtg gcggtgcgga
     7981 cacgcgccgg tctttttgac gtgagccaca tgggcgaaat cgtgattcgt ggtccaggca
     8041 cgttagagtt atgtcaaaaa atatccgcca atgacgtctc gcggatgaaa ctgcagcagg
     8101 cccagtataa tctcctcatg aacgacaagg gcgggatcgt agacgatgtt atcttttaca
     8161 agcttgaggc caaccatttt ttgatttgtg tcaatgcatc gaacagcgac aaagatttcg
     8221 cttggatcga ggcgcatgca gaaggcaacg tcgagttaga aaacgccagc tcgcgctatg
     8281 cgcaattggc cttgcaaggt ccgttggctg aaaagatctt gcagcccttg acctcactcc
     8341 ggctcggtga aattaggccg ttcttttttg cattcggaga tgtgtcgtcg attcgctgcc
     8401 tcgtggcgcg cactggttac accggcgaag acggctttga gttgtattgt ggctcgcctg
     8461 aggccgagac gttgtggaat gcgttacttg aaagtggggg gccagtcggg ctcgagcctg
     8521 cggggctggg tgcccgtgac acgttgcggc tggaaaaggc ttaccctctg tatggccacg
     8581 agctggacga tacaacaacg ccgctggaag cgggtttgga gtgggtgacg aagttggcca
     8641 aagggccatt cgtggggcgc gaagtcttgc tgaagcagaa agaagaaggg attcggcgca
     8701 agctcgtcgg aatggaactt ttagagcccg gaattgcgcg cgccgagtac gctctattca
     8761 aggatggaaa acccatcggc cgcgtgacga gtggcacgaa atctcccact ctcgccaaag
     8821 cgatagcgct cgcctatgtg accatcgagg aatctgtgcc ggacaacacg gttgaagtgg
     8881 aaattcgtgg gcgaaagacg cgcgccaaga tagttccttt gcccttttac cggcggtaac
     8941 gacataacca ttgagcaatg cgtaaggagg ttagtgatgg agtttcccga aggcctaaaa
     9001 tattccaaag aacatgaatg ggtcttggtt gaaggcaata ccgctacgat tggtatcacg
     9061 gagttcgccc aagaagaact aggcgatatt gtctacgtgg aactgccgga ggttggagaa
     9121 aaagttgtca aagatgatcc attcggagcc gttgagtcgg ttaaggcagt ctcggacgtc
     9181 tacgcgccgg tcggtggaac ggttctggag gtgaacgatg tgctgccgga taacccggag
     9241 acgatcaatg acgatccgta cggcgatggc tggatgattc gggttgaaat gacggatgaa
     9301 gaggacctca aggatttaat gacggcggag gagtacgccg aatacgtaga acaacagaag
     9361 gaagacgagg acgaggagga ggaagacgac gaggaggaag acgaagagaa ggaataactt
     9421 caagaaatgc gttacactcc ccacacctct tccgatcagg agcggatgct ccgtacaatt
     9481 ggaatcgact ccatcgagga actgtatcgt cacattccgg aaacgctccg caatcgtgca
     9541 aagatcgacc ttcctggagg tttaaccgag ctggcggttc gcagacgtct ggctgagcta
     9601 gcgtcgagca atgcgacggc gtcggagtgg agtttttttc tgggcggcgg gatctatcac
     9661 catttcatac ccagcgcggt ggacgccgtc atctcccgat cggagttttc cacttcctac
     9721 acaccctacc agccggaagt cagccagggt accctgcagg ctctgttcga atatcagacg
     9781 ttaatctgtc agcttaccgg gatggaaata tctaacgccg gagtttatga cggggcgtcc
     9841 gcggcggccg aagcggtcct catgtcgcgt cgtatccaac cgatgaatcg acggcgcgtc
     9901 ttgatgtccc gagcgctgca tccccactat cgagcggtta ttgcaacgta cttaagaaat
     9961 ttagacgaca tgcagctgga cgaggttccg tttgacggca gcggtgcaac ggatgtggca
    10021 gagttg
//
LOCUS       DASSWZ010000273         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_38695, whole genome shotgun sequence.
ACCESSION   DASSWZ010000273 DASSWZ010000000
VERSION     DASSWZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38695"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFN58_06625"
     CDS             complement(<1..581)
                     /locus_tag="VFN58_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET8917699.1"
                     /translation="MARRIDFATVVLPILGLGVMLLIWTILSTTVARDLPSPMKTWEE
                     SKQYILNPFFKDGEMNQGIGRFAFLSLIRVAKGFLLAIVIGTPLGFLLGLSAFFYRTF
                     DPVIQILRPISPLAWLPLGLVIFRQSEPAAIFTIAICAMWPTVINTAVGVRSISPEYL
                     NVGRVLKLSRMKTLRRIIIPGSLPYVFTGYRLSL"
     gene            complement(591..1868)
                     /locus_tag="VFN58_06630"
     CDS             complement(591..1868)
                     /locus_tag="VFN58_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET8917700.1"
                     /translation="MAKKGKQTTIKPFTRRNFLKLSGMSSLGLLFSNLPARWVGGVYA
                     ADGPETPNIRIGIIALTDNSSIVMAHELGLFKKYGIESTISKEASWAVIRDRLTLGEN
                     QATHMLFGMPFASTMGLLGSPKKPMIIPFVLNRNGQAITLKKELASKGIKTPQAIKPL
                     VDEAKKAGTPMTFAMTFPPGTHAMWTRYWLGAGGINPDKDVTLITIPPPQMVANMKVG
                     KMDGFCVGEPWNARAIADGIGYTAVTTQQMWKDHPEKVLAFTEEFAEKNPKTVKAILR
                     AMFESSQHIDKLENRPHMAEVVSRPQYINTQKEVILGRMLGDYDYGDGRKEKDPNYMT
                     FFVRNTNFPWKSHGVWWLSQFRRWGMVKEAPDYKGLVNQVLRPEIQREVAKEMGISMP
                     KEDMKKETLFDGVEFDPGKPEEYANKFPINNIG"
     gene            complement(1873..>3652)
                     /locus_tag="VFN58_06635"
     CDS             complement(1873..>3652)
                     /locus_tag="VFN58_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET8917701.1"
                     /translation="GWEAITDGDKERLKWAGVFFRKQTPGNFMMRVRMPNGITSATQF
                     RTLAEISEEFGKGFCDITTRQQIQLRWFKIENVPEIWRRLDRVGLVSLQTGMDNIRNV
                     VGCPVAGLTPNELFDASPVVRQFTGNFVGKKEYTNLPRKFNVTITACKENCTHAETQD
                     LALTPASKEIEGKVVNGFNVAAGGKMGSGGYRMASPLNLFVTQAEAAEICSQVVRTFR
                     DHGFRELRTKARLAFLIDDWGVEKFRKELERRAGRSLLTAGKDGRAEKHVDHLGVYRQ
                     KQPGLNYVGLAVPVGRITGEQLLEIADLAEKYGSGLIRLTIGQNVIIPNVPDALIGAL
                     TAEPLLKELRYDPSEIMRGLVSCTGMDYCHFALIETKQQALKTAWALEQELGKTKPIS
                     IHWSGCPAGCSNQAAADIGLIGKNIKMNGQVEEAVDVYLGGSHGPAANPPIKIMEDVP
                     CAELAQTLHGLIRHGAFKAMRQQLRKIPQTTSANGVHPLETSEPARPMIDPKDIAEGS
                     AKRVRTRGEEMAVFKCNGQLYGIQNICPHEGGQLCNGWIDGGEVVCPLHGYKFNLETG
                     ACSTDPKLKIKVFRLVSQGDQFTLEG"
BASE COUNT          758 a         1062 c          969 g          863 t
ORIGIN      
        1 agggagaggc gatatccggt gaaaacatac ggcagcgaac cgggtataat gatgcgcctc
       61 agtgttttca tccgggaaag cttaagcacc cgtccgacat tgaggtattc ggggctgata
      121 cttcgtactc ccaccgctgt gttgatcacc gtcggccaca tggcgcaaat ggcgatggtg
      181 aagatggcgg ccggctccga ctgcctgaag atgaccaacc ccagtggcag ccaagccaag
      241 ggagagatcg gccgcaaaat ctggattacc ggatcaaagg ttcgatagaa aaaagccgag
      301 agcccgagca gaaatcccag aggtgtgccg atgacaatcg cgagcagaaa tcccttcgcc
      361 acccggatca ggcttaaaaa ggcgaaccgg ccgattccct gattcatctc cccgtctttg
      421 aaaaaggggt taaggatgta ctgcttgctc tcctcccacg ttttcatcgg tgaaggcagg
      481 tcccgggcta cggtcgtgct gagtatcgtc cagataagca gcatgactcc gagcccaaga
      541 atgggaagga caacggtggc aaagtcgata cgtctggcca ttgtctacct ctacccgatg
      601 ttgttgatgg gaaacttgtt ggcgtactct tcaggcttac ccggatcgaa ctcaacgccg
      661 tcaaacaacg tctccttctt catgtcttct ttgggcatcg atatccccat ttctttggcg
      721 acctcacgct ggatctcagg acggagcacc tgattgacca gacccttata gtccggcgct
      781 tctttgacca ttccccaccg acggaactgg gagagccacc acactccgtg agatttccat
      841 gggaagttcg tgttgcgcac aaaaaaggtc atgtaattcg gatccttttc cttccttccg
      901 tcgccgtaat cataatcccc cagcatccgc ccaaggatca cctccttctg cgtattgata
      961 tactgcgggc gggaaaccac ttcagccatg tgcggccggt tctccaactt gtcgatgtgc
     1021 tggctggact cgaacatggc gcgaaggatc gctttcaccg tcttgggatt cttttcagca
     1081 aactcttcgg tgaaagccag gactttttcc ggatggtctt tccacatctg ctgcgtcgtg
     1141 acggccgtat agccgatccc atcggcgatc gccctggcgt tccacggctc gccgacacaa
     1201 aaaccatcca tctttccgac cttcatattc gccaccatct gcggcggcgg aatagtgatc
     1261 agagtcacgt ccttgtccgg gttgatcccg ccggctccaa gccagtaacg ggtccacatc
     1321 gcgtgggtcc ccggcgggaa agtcatggca aaggtcatcg gcgtacccgc cttcttggct
     1381 tcatcgacca gaggcttgat cgcttgaggg gtcttgattc cctttgacgc caactccttc
     1441 ttaagcgtga tcgcctgtcc gttgcggttc aggacaaaag gaataatcat aggttttttc
     1501 ggcgagccta atagccccat ggttgaggcg aagggcattc cgaaaagcat atgagtcgcc
     1561 tggttttctc ccaaggtgag ccggtcgcgg atgaccgccc atgaagcttc tttggaaatg
     1621 gttgactcaa tcccatactt cttaaacagg ccgagttcgt gggccatgac gatggatgag
     1681 ttatcggtca aagcaatgat gccgatacgg atgttcggag tctcaggccc atccgccgca
     1741 tagactccac cgacccaacg cgccggtaag ttgctgaaca gcaagcccag agaactcatc
     1801 cctgagagct tcagaaaatt acgtcgtgtg aacggtttta tggttgtctg ttttcctttt
     1861 tttgccacga tattatccct ccaaagtaaa ttgatcgcct tggctgacga gcctaaaaac
     1921 tttgatcttt agcttgggat ccgtcgagca cgccccggtt tcaagattga acttgtagcc
     1981 gtgtagcggg cagacgacct cgccgccgtc gatccacccg ttgcacaact gtccgccttc
     2041 gtgcgggcag atgttttgaa tcccatagag ttgtccatta catttgaata cagccatttc
     2101 ttcacctctc gtgcggaccc gctttgccga gccttcggcg atgtcctttg gatcgatcat
     2161 cggccgcgcc ggctcgcttg tttccaaagg atgtacgcca ttggcgcttg tcgtttgggg
     2221 aatttttcgg agctgctgcc gcatcgcttt gaaagcgccg tggcgaataa gtccatgcag
     2281 cgtttgggca agctccgcgc agggaacatc ttccataatt ttgatcggag gatttgcggc
     2341 cgggccgtgc gagccgccga ggtaaacatc caccgcttcc tccacctgtc cattcatctt
     2401 tatgttcttg ccgatgaggc cgatgtctgc tgccgcttgg ttactgcagc ccgccgggca
     2461 acctgaccaa tgaatcgata tgggcttggt ttttccaagt tcctgctcga gagcccacgc
     2521 agtcttgagc gcctgctgct tggtttcaat cagggcgaag tggcaatagt ccatacccgt
     2581 gcagctcacc agtccgcgca taatttccga aggatcgtag cggagctctt tgagaagcgg
     2641 ctcagccgtc aaggcgccga tcagcgcgtc cggaacgttg gggataatca cattctgacc
     2701 gatggtcaga cggatcaatc cgctgccata cttctcggcg agatcggcga tttccaacaa
     2761 ctgctccccg gtgatgcggc ctacgggcac ggcaagcccg acatagttga gccccggctg
     2821 tttctgccga tagactccca ggtgatcgac gtgcttctcc gctcttccat ccttgccggc
     2881 cgtcagtaac gaacggccgg cgcgccgttc cagctctttg cgaaacttct cgacacccca
     2941 atcgtcgatc agaaaggcga ggcgggcttt agtgcgcagc tcgcgaaacc catgatcgcg
     3001 gaaggtacgc acgacctggc tacatatctc ggcggcttca gcctgagtga cgaataaatt
     3061 caaaggcgac gccatccggt atccaccgga gcccatcttg ccgcccgctg caacgttgaa
     3121 tccgttgacg actttacctt cgatttcttt gctggcggga gttagggcga gatcctgagt
     3181 ctcggcgtgg gtgcagttct ctttgcaggc tgtgatcgtc acgttgaact tacgtgggag
     3241 gttcgtgtac tcctttttgc cgacgaagtt accggtgaac tgccggacca ccggcgacgc
     3301 gtcaaaaagc tcgttaggcg taagccccgc gacagggcaa ccgactacgt tacggatgtt
     3361 gtccatgccg gtctgcaatg agaccaaccc gaccctgtcg agccgccgcc agatctcggg
     3421 cacattttca attttaaacc agcggagctg aatctgttgg cgggtggtga tgtcacagaa
     3481 ccctttgccg aactcctcgc tgatttccgc gagcgtgcgg aactgcgtcg cgctggtgat
     3541 gccgttcggc atacgcaccc gcatcatgaa gttgcccggc gtctgcttgc gaaaaaaaac
     3601 tcccgcccac ttcagtcgct ccttgtcgcc gtcggtaatc gcctcccagc ct
//
LOCUS       DASSWZ010000274         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1114420, whole genome shotgun sequence.
ACCESSION   DASSWZ010000274 DASSWZ010000000
VERSION     DASSWZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114420"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1697
                     /gene="leuC"
                     /locus_tag="VFN58_06640"
     CDS             300..1697
                     /gene="leuC"
                     /locus_tag="VFN58_06640"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET8917702.1"
                     /translation="MAGRTLYDKIWESHSVGILPTGQTQLFIGLHLIHEITTAPAFDM
                     LREQGMDVAFPERTFATVDHIVPTHVRTRPFLDSEAEELHQALERNVAQFGIEFFHLD
                     HEHQGIVHVIGPQLGLTQPGMTLACGDSHTSTHGAFGTLAFGIGTSQVRDVLATQTLA
                     MDKLKVRRIGVDGVLGTGVYPKDVILSIIRRLGIGGGKGFAYEYGGAVLDNMNMEERM
                     TVCNMSIEGGALVGYVNPDQTTFDYLKGRPYVPTGEAFDRAVAYWKSVASDPDAKYDD
                     VVSFEGKDIEPTVTWGINPGQAVGISEKLPKPQDYSDPEGARKAYEHMGWHPGAPILG
                     TPINVAFIGSCTNSRITDLRAAAKIAKGRKVKPGIRALVVPGSAEVKKLAEQEGLHEI
                     FKEAGFEWRDAGCSMCLGMNPDKLIGREISASSSNRNFIGRQGSPEGRTLLMSPAMVA
                     AAALNGKVVDVRDYQ"
     gene            1708..2322
                     /gene="leuD"
                     /locus_tag="VFN58_06645"
     CDS             1708..2322
                     /gene="leuD"
                     /locus_tag="VFN58_06645"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET8917703.1"
                     /translation="MSEIVKITKITGTAVSIRGNDIDTDRIIPARYLKEVTFARMGDY
                     PFFDERFDASGKKKDHPLNDPEYKGASVLFVNKNFGCGSSREHAPQALYRFGIQAIVG
                     ESFAAIFAGNCTIMGVPTVTVSAKDMEQLMKSVEENPRTEYTVDLESKTLLYGNHKIA
                     IDLPESYRNALTTGSWDSTTLLRDNLEQVKKTASKLPYMSRFAS"
     gene            2373..3071
                     /locus_tag="VFN58_06650"
     CDS             2373..3071
                     /locus_tag="VFN58_06650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8917704.1"
                     /translation="MAERIVSMHAAYKSGKEAVDGFLSRPTAGNPRTAIVLIHGYRGL
                     DDGQRAVTRRFAQEGFVCLSPDLFHGKIFNTLDECALQKTSLDIPRAVEKVIDSVPYL
                     RKLPWVGKKNIAVLGFCMGGGLALYAVARSRAFAAGVIYYQSLFPDPEDLKGITAPLL
                     CLYGTHDPGTTKTEIDMFRETLEKYKKKYEIEMYDGAGHAFLNNPQTKSAANREAAAK
                     SVIKTSKWLRKHLA"
     gene            3093..3398
                     /locus_tag="VFN58_06655"
     CDS             3093..3398
                     /locus_tag="VFN58_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET8917705.1"
                     /translation="MANKWDERKKAQEDEYFVKKERELLANLKAKQEAETKTSLQKTC
                     YMHCPKCGAPLKARSFQKVEIDQCTGCNGIWLDAGELDQVAGKEEGSWLGKFWQKNS"
     gene            3482..>4005
                     /locus_tag="VFN58_06660"
     CDS             3482..>4005
                     /locus_tag="VFN58_06660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET8917706.1"
                     /translation="MKNVKTFLTAGLFSLAVAIAGLAFAPSTALAQAAQKYNDEGKKI
                     VPYVPTPQEVVERMLELAQVKKGDVVYDLGSGDGRIVVTAAKKYGVKAIGFEIDPERI
                     KESAENIKKAGVGDLVEIRQQDIRTVDLSQATVLTMYLLPEVNLMIRPNIWKQMKPGS
                     RVVSHDFDMGDWKPL"
BASE COUNT          998 a          977 c         1151 g          879 t
ORIGIN      
        1 gatccggaag tacgaaagct cgcggagaaa tacggcgatc cggataatct tcttagagag
       61 gactggattc cgcagattcc aggtataacc gcggccggct cttacgatga gtatgcgaga
      121 aatcctgggg cgtggatcta caatcagaag gcgtgaagta ccctgggagg tttcaccctc
      181 accgtcgagg gagaggatgt aaaacttcac tcgctgtcta agatctattc ctgcggagcg
      241 cctcttgaag tcgtggatgg gggtggatat gatccgagcg aagaaggaga tttttaggaa
      301 tggccggacg gacactctac gataagattt gggagtccca tagcgtcggg attctgccta
      361 ccgggcaaac gcagctcttc atcgggctgc acctcattca cgaaattacc acggcgccag
      421 ctttcgacat gctgcgcgag cagggtatgg acgtggcatt tccagaaagg actttcgcca
      481 cggtcgatca catcgtcccc acccacgttc gaacccggcc gtttcttgat tccgaagccg
      541 aggagttaca tcaagccctg gagagaaacg ttgcgcagtt tggcatagaa tttttccatt
      601 tggaccacga gcaccaaggc atcgtccacg tcatcgggcc gcagttgggt ttgacgcaac
      661 ccggaatgac gcttgcctgt ggtgacagcc acacgagcac gcatggcgcc tttggcacgc
      721 ttgccttcgg gattggaacc agccaggtgc gtgacgtttt agccacccag acattggcga
      781 tggataagct caaagtgcga aggatcggtg tcgacggagt ccttgggacc ggcgtttacc
      841 ccaaggacgt aatcttaagt attatacggc gattgggtat tggcggcggg aaaggcttcg
      901 cttacgagta cggcggtgca gtcctggaca acatgaacat ggaagaacgg atgaccgtct
      961 gcaacatgag catcgaaggt ggtgccttgg tcggctatgt taatcccgat cagacgacct
     1021 tcgactatct caaggggcgc ccttatgtcc cgaccggcga ggcatttgac cgcgcagtag
     1081 cttactggaa gtcggtggcg tcagatcctg atgccaagta cgacgatgtg gtaagctttg
     1141 aaggaaagga catcgaaccc accgtgacct gggggatcaa ccctgggcag gcggtgggca
     1201 tctccgaaaa acttcccaaa ccacaggatt actccgaccc ggagggagcc agaaaggcct
     1261 acgagcacat gggttggcac cccggagcac cgatactcgg cacgccgatc aatgtggcgt
     1321 ttatcggctc ttgcaccaat tcaagaatca ccgatctgcg cgcggccgca aagattgcca
     1381 aaggtagaaa agttaaaccc ggtattcgtg ccttggtggt tccgggttcg gctgaagtta
     1441 agaaacttgc cgagcaagag gggttgcacg agattttcaa ggaagcggga ttcgaatggc
     1501 gtgacgcggg ctgctcgatg tgcctgggaa tgaatcccga caagctgatt ggccgcgaaa
     1561 tttctgcctc atcttcgaac cgtaatttca ttggccgcca ggggagtcct gaagggcgta
     1621 ctttgctcat gagtccggcg atggtcgcag cggcagcctt gaacggcaaa gttgtcgacg
     1681 tgcgcgatta tcaatagacg agggaccatg agcgaaattg ttaagatcac gaaaatcacc
     1741 ggcactgccg tttccatcag aggcaacgac atcgatactg accggatcat cccggcccgc
     1801 tatttgaaag aagtgacgtt tgcgcgcatg ggggactatc ctttttttga cgagcgcttc
     1861 gatgcgtcgg gcaagaaaaa agaccatccg ttgaacgatc cggaatacaa gggcgcttca
     1921 gttctcttcg tgaacaagaa tttcggttgt ggctcgtcac gtgagcatgc gccgcaggcg
     1981 ctttaccgtt tcggcattca agccattgtc ggtgaatcct tcgcggcgat ttttgccggt
     2041 aattgcacga tcatgggagt tcccacagtt actgtgagcg caaaggatat ggaacagttg
     2101 atgaaaagcg tcgaggaaaa cccgcggacc gaatacacag tcgatctgga aagcaaaacc
     2161 cttttgtatg gtaatcacaa gatcgccatc gatttacccg agagctatcg caatgccctt
     2221 acgaccggct catgggattc caccactttg ctgcgggaca atctcgaaca ggtcaagaag
     2281 actgcgtcta aactgcccta catgagtcgc tttgcgagct gaaattcagt ttttccactg
     2341 ctgcaaattc tggtttctct ttcaagtgag acatggctga aagaattgtc tccatgcatg
     2401 ccgcctacaa aagcggtaaa gaagccgtgg acggttttct ctctaggccg acggcgggta
     2461 atccgcgcac ggctatcgtt cttattcatg gctatcgggg cctggacgat gggcagaggg
     2521 cggtgacgcg cagatttgcc caggaggggt tcgtctgttt atccccggat cttttccacg
     2581 gtaagatttt caatactctc gatgagtgcg cgttgcaaaa aacttctttg gatattcctc
     2641 gagcggtaga gaaagttatt gatagcgttc cctaccttcg taaattgccg tgggtcggca
     2701 agaaaaacat tgcggttctt ggcttttgca tgggcggcgg cctggcgctt tacgcggtag
     2761 cccggtcgcg tgcgtttgca gcgggagtga tttactacca gagccttttt cccgatccgg
     2821 aagatctgaa gggtatcacc gctccactcc tgtgccttta cggtacgcat gaccccggca
     2881 ccacgaagac ggaaatcgac atgtttcgag aaactctgga aaaatacaaa aagaaatatg
     2941 aaatcgagat gtacgatggg gccgggcatg cttttctcaa taatccccag acgaaaagcg
     3001 ctgccaaccg agaggccgct gcaaagtccg tgatcaagac cagcaagtgg ctccggaagc
     3061 atctcgccta gactagggtc aaggagggaa tcatggcgaa caaatgggat gagagaaaaa
     3121 aggcgcaaga ggacgaatac ttcgtcaaga aggaaagaga actgctcgcc aacttaaagg
     3181 cgaagcaaga agctgagact aaaacatcgc ttcagaagac ttgttacatg cactgcccaa
     3241 agtgcggcgc gccgttgaaa gcgagaagct ttcaaaaggt tgagatcgac caatgcacgg
     3301 gatgcaacgg catttggctg gacgccggcg agctcgacca agtggcaggc aaagaagagg
     3361 gcagttggtt gggcaagttt tggcaaaaga acagctaggt gttggtttgt ctcttgacag
     3421 aaaagggaca actttcggat agtcgcgttc gattcctatt tgaaccaagg aggggttcca
     3481 gatgaaaaac gtcaaaactt ttttaacggc gggcctgttt agtctagcag tcgcgattgc
     3541 gggactggca tttgcgcctt caacggcctt ggctcaggcg gcgcagaagt ataacgatga
     3601 aggcaaaaag atcgttccct atgtcccgac gcctcaggaa gtcgtggaac gcatgctcga
     3661 gctggcacag gtcaaaaagg gtgatgtggt ttatgatctt ggctccggcg acggaaggat
     3721 cgtggtaacc gcggccaaaa aatacggtgt caaggccatc ggttttgaaa tcgatccgga
     3781 acgcatcaaa gaatccgccg agaatatcaa aaaggcaggc gtcggtgatt tagtggagat
     3841 ccgccagcag gatatccgga cggtggatct ctctcaggcg acggtgttga cgatgtatct
     3901 tcttcccgaa gtgaatctta tgatccggcc aaatatttgg aagcagatga agcccggttc
     3961 ccgggtggtt tcccatgact tcgacatggg cgactggaaa ccgct
//
LOCUS       DASSWZ010000275         4997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_448941, whole genome shotgun sequence.
ACCESSION   DASSWZ010000275 DASSWZ010000000
VERSION     DASSWZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448941"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFN58_06665"
     CDS             complement(<1..227)
                     /locus_tag="VFN58_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917707.1"
                     /translation="MRLFFLALGTIVLVVVLASAVAANEAKPPEVKLAAAFQLPQLPG
                     LSGPSADWRQWDSFFTFVVKQFGQELSGDLK"
     gene            complement(382..588)
                     /locus_tag="VFN58_06670"
     CDS             complement(382..588)
                     /locus_tag="VFN58_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917708.1"
                     /translation="MGVASDIINLAFGLLLGAIAVAVALAVGLGSRDIAAREVETLIR
                     SMKSNGAKEAQRQEESARGGGLVG"
     gene            complement(589..1929)
                     /locus_tag="VFN58_06675"
     CDS             complement(589..1929)
                     /locus_tag="VFN58_06675"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET8917709.1"
                     /translation="MEATLDQMRNVIGAYVPNLLGALAILVIGWLVAWIIATLLNAAL
                     RRTELDNRLARWIAGEESVRHIPLETWISRGVFYLLMIFVLVAFFQALQLTLPSEPLN
                     RLLVQLFEFVPKVIGAGVLLLIAWVLATIVRALILRVFKAAKIDSRLAEQAEGTKQVS
                     LTQTIADTSYWLIFLLFLPAVLDALGLVGLLTPVQGMVNKVLNYLPNIFTAALILAIG
                     WFVARIVRNVVTNFLVALGADQMGQRVGLTPVFGGQTLSGVVGLLAYVLILVPVLVSA
                     LDALALEAITRPTSNMLNTILNALPDIFAAGLIISVAYIAGRLVAGLVTHLLSGIGFD
                     AILVRLGLSRAPAPGRRTPSEVVGLLVFLAIILVAATEAARQLNFIVLADLITRFMIF
                     AGEVILGLIIFAIGLYLANLAGSVIETSRATHAGVLAVAARVAIIVLAGAMALR"
     gene            complement(2019..2945)
                     /locus_tag="VFN58_06680"
     CDS             complement(2019..2945)
                     /locus_tag="VFN58_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET8917710.1"
                     /translation="MRKILLALVLSFFYAHMAWALTIATGPSDGSYFQIAQDIKNVAG
                     KEGIDLQVMATKGSLENLDLLSSGKADLAIVQLDALRFISDVVKQDLNVDVFDKIKVV
                     LNLYPEEIHILTNKKDIQTFYNLQGKRVSVGPPGGGTAVTAAVLFNIYDIKSTVSEEP
                     FEDAIKKIDQGSLDAVIFVGGAPVPFIAKLDNRFHFVRLPNNPILDQIYLRIPLGKQQ
                     YSWAGADTETYAVPAAIMGLDKRDEKYVSQMQQLVLSVLNSSEYLNASGHPKWKSSII
                     RTYFPSRGYEPTNQVIQIFNLLDKQGYKVIKK"
     gene            complement(3040..4929)
                     /locus_tag="VFN58_06685"
     CDS             complement(3040..4929)
                     /locus_tag="VFN58_06685"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET8917711.1"
                     /translation="MSDTNVNTPRLKPEYSNRSDRDLQRCMHRARLRYWFRVIAGALV
                     LSGCASGATAAEAAKPAEVSKVAAPKEAELVVWNQPITVFRASFAGLTPEERAAQAAE
                     RIQDLPLGSESAEIKFEPAKIGAAEGIAFSINSRQLFFLTAGDLAPDSNQTLNTQAQT
                     IVTALRAALRARIEQRQWSTLLMSVARVLIATLILLGLMWLWRRVKIWLLKALGTTAL
                     LPEKYLRFFAVDLRMHILQGGRAAIRFVSAIVVLILGYWWLVYSLEQFPYTASLGEKL
                     ETFLVRLFLDFGHGVVAAIPGLVAVAIIILVAIWFARVINTFFKEIEKGTLSLPWLEK
                     ETARTTQTLLIVAVWLFALVVAYPYIPGSGTEAFKILSILIGVMIALGSTGLISQIMS
                     GLFVTYSKGARPGDHVRIGDVEGEVLNVGLLATKLKTLNQEEITVPHSLLVGATTTNY
                     SRLTSEEGMLITVSLTIGYDVPWRQVNELLLLAASRTAGIYQEPPPRVLQVELSDFYV
                     KYHLLAHLEDGKNRAEVLSDLYAQIQDAFNEYGAQIMSPHFESQPKKPVLVPKSGWYA
                     PPVSSPGSGMTREPMEVEPREIEARENVSAAPPARPSSPPAAAKSERRRHMPPRNPRP
                     KPPNI"
BASE COUNT         1186 a         1448 c         1285 g         1078 t
ORIGIN      
        1 tccttcaagt cgccgctcaa ctcttgaccg aattgtttta cgacgaacgt gaaaaacgaa
       61 tcccactggc gccagtccgc cgagggaccg gacaatccag gcaattgcgg cagttgaaac
      121 gcagctgcga gtttcacttc aggtggcttc gcttcgttcg ccgctaccgc tgacgcaaga
      181 acaacaacca gcacgattgt tcccagtgcc aaaaagaaga gccgcatgac tctgactccc
      241 gtcactatcc taaaagcaaa cgcatcgcta acaactcacg accgactgca aagtcacgaa
      301 tagtctacag ctctggcccc atagagatcg cctcgatttc ggaccgactc ctcttcaagc
      361 tccaagcggg gcctgtgccc tctagcccac taatccccca ccccttgctg actcctcttg
      421 gcgttgagcc tccttagcac cgttgctctt cattgaacgg atcagcgtct cgacttcccg
      481 ggccgctata tcgcgcgagc ccagtcccac cgccagcgcg actgccaccg caatagcacc
      541 aagcagcaga ccgaacgcca agttgatgat gtcgctggct acgcccatct accgcaaggc
      601 catcgctcca gccagcacga tgatggcaac acgagcggcc acggccagca cgccagcatg
      661 agtcgctcga ctggtttcga ttacgctgcc ggccaaattt gctaaataga gaccgatcgc
      721 aaaaataatc aatcccaaaa taacctctcc ggcaaagatc atgaagcggg tgattaggtc
      781 ggcgagcaca atgaagttta actgccgcgc ggcttccgtg gcggcgacaa gtataatagc
      841 cagaaagacc aggagcccga caacttctga cggcgtccgt cgtccagggg caggagcccg
      901 actcaaccca agccgaacca agatggcgtc gaacccgatg ccgctaagca agtgggtgac
      961 gaggcccgct accagcctgc cggcgatata tgcgaccgat atgatgagac cagcggcaaa
     1021 aatgtccggc aaggcgttta gaatcgtatt cagcatgtta ctcgtcggcc gcgtgatcgc
     1081 ttcgagcgcc aaggcgtcga gggcggaaac gagcaccggc accagaataa gaacataggc
     1141 gagcagcccg acgaccccgg aaagggtttg tcccccgaaa accggcgtca atccgaccct
     1201 ctggcccatc tgatccgcgc ctagagcgac caaaaagttg gtcacgacgt tgcgcacaat
     1261 gcgtgcaaca aaccagccga tggccaatat cagcgcggca gtgaaaatat tgggcaaata
     1321 gttcaacact ttgttgacca ttccctgaac cggcgtcagc agtcccacaa gtcccagagc
     1381 gtcgagcacg gcgggcaaaa aaagtaggaa aataagccag taggaggtgt cggcgatggt
     1441 ctgcgtcaag gaaacttgtt tggtaccttc ggcttgctcc gccagtcgcg aatcgatctt
     1501 cgcagccttg aagactctca ggatgagcgc gcgaacgata gtcgccagca cccaggcgat
     1561 cagcaataga acgcccgcac caatcacttt cggaacaaac tcgaacaatt ggaccaacag
     1621 tcgattaagc ggttcgctgg gaagggtcag ctgcagcgct tggaagaagg caaccaggac
     1681 aaaaatcatg agcagataaa acaccccccg gctgatccaa gtctccaagg gaatgtgtct
     1741 taccgactcc tcgccggcga tccagcgagc gagccggtta tccagttcgg tgcgtcgcaa
     1801 agctgcatta aggagcgtgg cgatgatcca ggccacgagc cagccgatga ctagaatggc
     1861 gagggcgccc aaaagattgg gaacgtaggc gccgataacg ttgcgcatct ggtctagagt
     1921 agcttccatg ttccctccta tgtgtggtat tttttaatgc atcactgtat ggtgctcctt
     1981 accaaagatc tgccggctct gcccaccacg acaaacggtt acttttttat gactttgtat
     2041 ccttgcttat cgagcaggtt aaaaatctgg atcacctgat tggtcggttc atagccacgg
     2101 gagggaaagt aagttcgaat gatggagctc ttccacttgg gatgtccgct ggcatttaga
     2161 tattcgctgc tgtttaagac agagagaacc agttgctgca tctgactgac atatttttca
     2221 tcccgtttgt ccaatcccat aattgcggca ggtaccgcgt aagtctccgt atcggctccg
     2281 gcccaactgt attgctgctt tcctagtgga attcgcaaat agatctggtc cagaatcgga
     2341 ttgttgggca gccggacgaa gtgaaatctg ttgtctagtt ttgctatgaa gggcaccggg
     2401 gcgccgccga caaaaatcac cgcatccagg ctaccctgat cgatcttctt gatggcatcc
     2461 tcaaaaggtt cctcggagac ggtcgatttg atgtcataga tgttaaatag caccgccgcc
     2521 gtaacggcgg taccgccccc cgggggtccc accgaaaccc gctttccctg caagttataa
     2581 aaagtctgaa tgtctttctt gttggtgaga atatggatct cttccgggta aagattcagc
     2641 acgactttga ttttgtcgaa gacatccaca ttcaggtctt gcttgaccac atcagaaatg
     2701 aaccttagag catccaactg aacgatcgcc aggtccgctt tgccactact taaaaggtcg
     2761 aggttttcaa gagaaccttt agtggccatg acctgaaggt cgatgccttc cttcccggcg
     2821 acatttttga tgtcctgcgc gatttgaaaa tagctgccgt cactcggtcc cgtggcaatg
     2881 gtaagagccc acgccatgtg cgcatagaag aaagagagga ccagcgccaa taatattttt
     2941 ctcatctgtt ttctcctttt ccttctctca ttcacctcta caaccccgct gaacttttgt
     3001 cctattcgga atccagccgg gactcctggt ccgccgcagc tagatgttcg gtggcttcgg
     3061 tctgggattt cgagggggca tatgcctcct acgttcgctt tttgctgccg ctggcggcga
     3121 actgggcctg gccggtggtg ctgcgctcac attttctctc gcttcgatct cccttggctc
     3181 gacttccatg ggctccctcg tcatgcccga acccggcgat gatacaggcg gagcatacca
     3241 tccagatttc ggcaccaaga ccggcttttt cggctgcgat tcgaagtgcg gcgacataat
     3301 ttgagcgccg tactcgttga acgcgtcttg gatctgcgcg tacaagtcgg agagaacttc
     3361 agcgcggttc ttgccatctt ccaggtgcgc caataagtgg tacttcacgt agaaatcgga
     3421 cagctccacc tgcaagactc gcggcggcgg ctcctggtaa ataccagctg tccgcgacgc
     3481 tgccaaaagc aagagttcat tgacctgacg ccaaggaaca tcgtagccga tggtcaggga
     3541 aaccgtgatc agcattccct cctcgctggt caaacggctg tagtttgtcg ttgttgcgcc
     3601 taccagcaga gagtgcggga cagtaatctc ctcctggtta agagttttca gcttcgttgc
     3661 aagcaagcct acgttgagga cttcgccctc gacgtccccg atgcgcacat ggtccccggg
     3721 cctcgcccct ttggaatagg taacgaataa cccgctcatg atctggctga tgaggccggt
     3781 cgacccgagg gcgatcatga ctccgatgag aatgctcaag attttgaacg cttcggttcc
     3841 tgagcctggt atatatgggt aggcgaccac taaagcgaac agccaaacgg caacgatcaa
     3901 cagcgtctgc gtggtgcgcg ccgtttcctt ttccagccag ggtagcgaaa gtgtgccctt
     3961 ttcaatctcc ttgaaaaaag tgttgattac ccgggcaaac catatggcga ccaggatgat
     4021 gatcgccacg gcaaccagtc ccgggatggc cgctaccacg ccatggccga aatccaagaa
     4081 caatcgaacc agaaacgttt cgagcttttc gcctagagaa gcggtgtacg ggaattgctc
     4141 cagggaatag actaaccacc aatagccgag tataagaacc acaatggcgg agacaaaacg
     4201 aatcgcagcc cggccccctt gaagaatatg catgcgcaga tccacggcaa aaaagcgcag
     4261 gtatttctcg ggcaataagg ccgtcgttcc caacgccttg aggagccata ttttcacgcg
     4321 tcgccacagc cacatcagac ccaacaagat caacgtcgcg atcaatacgc gagccacact
     4381 catgagcaga gtgctccact gtctctgctc tatgcgcgcg cgcaaggccg cccgcagcgc
     4441 cgtcactatg gtttgcgcct gtgtatttaa cgtctgattt gaatcagggg ccagatctcc
     4501 tgcggtcagg aaaaataact ggcgtgaatt aatgctaaac gcgataccct ctgcggcgcc
     4561 gatctttgcc ggctcgaact tgatctcggc cgattcactt ccaagcggca aatcctgaat
     4621 gcgctccgca gcttgtgccg cccgctcctc cggggtgagc ccagcgaaag acgcgcggaa
     4681 taccgtgatg ggttggttcc aaaccactag ctccgcttcc ttcggcgcag ccaccttgga
     4741 cacttccgct ggtttcgccg cctcggcagc tgtagcgccg gaagcgcagc cggagaggac
     4801 cagggctccc gcgatcacgc ggaaccaata gcgcaaccga gcccgatgca tgcagcgctg
     4861 caaatctcta tcggaacgat ttgaatactc aggttttagc cgaggagtgt ttacattagt
     4921 gtccgacata atcgcctgct ctttctttgt tcatgcgttt gccgcgtacg catcactatt
     4981 tcgaacaacc aggtgtt
//
LOCUS       DASSWZ010000276        11705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_308246, whole genome shotgun sequence.
ACCESSION   DASSWZ010000276 DASSWZ010000000
VERSION     DASSWZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308246"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..718)
                     /locus_tag="VFN58_06690"
     CDS             complement(317..718)
                     /locus_tag="VFN58_06690"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET8917712.1"
                     /translation="MPFELLNPQGLERPVGYTHVAKVTGGSIVYVAGQAPFDEHGQVV
                     GRGDFVTQFAQVIQNLKTAVEAAGGRPDQYAVLTIYITDLQAYINNKHALGGTYREVF
                     GKYFPAITLVEVVSLYNRDCMVEISGIAVID"
     gene            complement(723..1754)
                     /locus_tag="VFN58_06695"
     CDS             complement(723..1754)
                     /locus_tag="VFN58_06695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET8917713.1"
                     /translation="MKIDIEFNSGAQLPMAAIPELAQLAEKHGFDCAWGGEANNKDPT
                     VMLAAIAAVTTRLKVGSAVYHILGRTPATLALQAVGLDELSSGRFFLGIGSSNPTIAK
                     WHGQTFDHPLGRVEEYIEIVRAAMRGDKLNFYGKFFTAENFKMAFRPSGRTIPIYLAA
                     FGSKMTRLAGRMTDGVLINMANPIEIRRIAEEVKRGAEEAGKDPSKIEIICKIRCAIA
                     PSHQAAREALSHALTYYALADYYRDLLGRMGFAAEVEAMRAAWKSGGFHAARKFVTDR
                     LFEGLPLVAATSVDEIVEQIKPYQQAGATRIILPYVAASDDVVSEVKHFITCWSAAAT
                     ELTLMRKGD"
     gene            complement(1785..2390)
                     /locus_tag="VFN58_06700"
     CDS             complement(1785..2390)
                     /locus_tag="VFN58_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET8917714.1"
                     /translation="MKRFDCLKTVAVAATDALVVTSAGAVTLEWNALRPDDGNFRVRT
                     LGLCSSIALGMALGLPHRKVIALDGDGSLLMNLCSLPTLARMQPKNLVHIVFDNGVYE
                     ASGSKKTATAVGTDLVGMARAAGIAQALWAHSVDEFAKVATDALNGHELFFIGAKVST
                     ERTPVLPYPIDEVENKYRFIRHVEKTEKIAILKTNLPSSYT"
     gene            complement(2437..2958)
                     /locus_tag="VFN58_06705"
     CDS             complement(2437..2958)
                     /locus_tag="VFN58_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8917715.1"
                     /translation="MTEEDAAKTIFNGLKAAGINLVATLPDINLAHLLHEVEADRDVI
                     HVPLCREEEGIGICAGAYLVGKKCAAIMQNGGFFNSNNAIVTTLLQYQIPLLLLIYYA
                     GDIGDRTFSTSGSVTEPVLQALGIRHYIMREPGDAPELIKRAQILAEDSRRPVALLLT
                     KELLGRRASLKSL"
     gene            3123..3725
                     /locus_tag="VFN58_06710"
     CDS             3123..3725
                     /locus_tag="VFN58_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET8917716.1"
                     /translation="MIKLYDFKSSPNCQRVKVVLAEKNLPYEIVPIDLRKQEQKTPEY
                     LKLNPDGKVPVLVDDGTVLYESCIINEYLNEKYPNPPLMPADLGKRAKARILTDYGLA
                     RLDGPYQKLRVEVTKDPKEQNQQVIDTAKSDLRKLLQRLEDAIGDQPYLVGDFSLADA
                     DLLPRFTRLEGFGVLPDASLPRLGKYLQRMKERPSVKAIL"
     gene            3777..6215
                     /locus_tag="VFN58_06715"
     CDS             3777..6215
                     /locus_tag="VFN58_06715"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET8917717.1"
                     /translation="METPKSGLIPTVFSALLRPFICFLDRGSFPKYRGSLQLAGLRDK
                     VKVCWGAYGIPHAYAGNEEDLFLAQGYLHAQERLWQMDMSRRFLSGRLAEIFGNFPVP
                     WKELSSQFRGQNSTDFDYFMRLMGIQHSAVASLQLASEDEHRRLHAYSQGVNRYIEQC
                     GRRPPWEFRLLRYDPEPWRPDDSLIIGKGFAFLLSTALFTRLNMIAIAAKLGDHQEML
                     RSLCPTYPDDGPTITQAVWNSAQNIWQFMNGAYARSDWSAAGHGSNNWVVAPSRSATG
                     RAILCNDPHLRLTIPSTWYLMHLKAETSTTRPDGYEVWGASIPGSPCVQLGYNRWISW
                     GVTAALCDDVELYREKPHPLDPDRYLTRDRWLTMARREEIIKVRRSGETKKIVRSTCH
                     GPVLSDFSNQLNPSPILSLRWTAHEPSQEFRCLYGVNQARDWREFLDSLAYHSGPTLN
                     YVYADSGGNIGYSLAGRIPLRRGIPSLLPLDGWIEDNDWLGYIPFSELPRIYNPPEGV
                     IATANNRIVDSSYPHYLSHFYEPPSRIRRIKELLAAKKSFSISDMESMQNDRVSLYAT
                     ELIATLKSDLARAPEKSGQLRTAADRLINWDGKCDEASVESAIFHVFHYRLMANLLVP
                     TLGEDLFSAYLEIFNQSLMPTHQILRDPDSRWFSTTSRQDVVAKSLREACAELKTSLG
                     DEVELWQWGKIHSLMLSHALGHIKLLRPLLNLGPFPSPGDGTTINTGFYRHSSPYAHT
                     VGASLRFIIDVGEWQQSGFVLASGQSGRLFSPHYGDQTALWGAGRYLKIGTQDEQTPF
                     ESGLFLAPPSKPLP"
     gene            6288..6947
                     /locus_tag="VFN58_06720"
     CDS             6288..6947
                     /locus_tag="VFN58_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET8917718.1"
                     /translation="MSLKLLIADDERLFRQSLKILLETAKDIKVVAEAADGQEAVVKC
                     RETEPDIALLDVRMPKMDGIKAAKLIAALTPRTKILMLSIQDDDEKIASAIKAGAKGY
                     ILKDTDRAEFIEIVRRAHTGKIQPSPYLAQLTLERPSDDEGPPNNSRERQFSEKYKLT
                     DLELKVLQLVAEGLNNEEISHVTHVARETIKGYLKGLFRKLQVKNRTEAAVLAVREGI
                     D"
     gene            7146..7634
                     /locus_tag="VFN58_06725"
     CDS             7146..7634
                     /locus_tag="VFN58_06725"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7293..7295,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HET8917719.1"
                     /translation="MTGFAVALEVGQKAPDFELESTKGGKLSLSSLKGKNVLVNFYVL
                     DFSPTUIKDLQASGSDNYPMFQAENTEVVGISANAPFSQKAFADFAKINYPLLSDRDG
                     KVMQAFGVYDEPRRIAKRAYVIIDKDGVVRYLNIRPTNTEKDLLSTEQLLNEVKKVNK
                     GS"
     gene            7670..8122
                     /locus_tag="VFN58_06730"
     CDS             7670..8122
                     /locus_tag="VFN58_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET8917720.1"
                     /translation="MVRGKFLIAAVIGFMTLLASSHVFAQADVIEKRQAAMKANSADA
                     KAIKAAVESKDYATIEVKAKDIMATAEKIPALFPKGSTTGKTKATDAIWEKSDDFAKA
                     AKNLGKAASELAATAKAKDDAEIGVKVKALGDACGSCHKAFRAEKYAE"
     gene            8259..9266
                     /locus_tag="VFN58_06735"
     CDS             8259..9266
                     /locus_tag="VFN58_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917721.1"
                     /translation="MKRPVFFCSIVYFTWLFVLISSASGQLTRLNVGYSAVSADQLPA
                     WVAKDAGIFARNGLDVQLIFFTGGTTAILALVSGDVPITQVSGPGLVNSALAGSDAVF
                     VAAGIISLNYVLMGKPGIKTAEQLRGGTLAISRFGSATDSIARFALRKIGLTPGKDVT
                     IVQVGSGPERLSAVLTGRATAAVINPPSSFIAEKRGLAVVADVAQMGLVFQHTGVATT
                     RRFIKEHPDTVRRYVRAHVEAVQKMWTDREATIKALSKYMGSGVDRETLEKSYENVMT
                     ESFYPKKQYPSLEGLKTVLEDVSERDPRAKTAKPEQFVDFSFIKELDGSGVIDGLYKK
                     K"
     gene            9289..9549
                     /locus_tag="VFN58_06740"
     CDS             9289..9549
                     /locus_tag="VFN58_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917722.1"
                     /translation="MISTKLGEDASSDIQDWANRVAGLVDSAVTVDVYYDGDSNTYIL
                     RLAKAARVLVFRLSESQVSSSGRETECERTLQRKVKDLWNLI"
     gene            complement(9578..10555)
                     /gene="hemB"
                     /locus_tag="VFN58_06745"
     CDS             complement(9578..10555)
                     /gene="hemB"
                     /locus_tag="VFN58_06745"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET8917723.1"
                     /translation="MDFPAYRPRRLRRNEKIRELVRETTLSPKNFIYPIFVGPGKNRA
                     QPVSSMPGVSQLSVDRAVTECEEAQALGVPAIILFGVPEHKDSVGTEAYADTGVVQQA
                     IRAVKEKVPGLLIMTDVCLCEYTDHGHCGVIKNGDVDNDSTLELLAKEALSHARAGAD
                     IVAPSDMMDGRVGAIRKSLDENGFEQIAIMAYAAKYASGFYGPFREAAESTPQFGDRR
                     SYQMDPANADEALREVELDIREGADIIMVKPAMAYLDIVYRVKQKFGYPVAAYNVSGE
                     YSMIKAAGRNGWIDEERIMMEVLFAIRRAGADMILSYFAKDVARLFAGK"
     gene            complement(10569..11312)
                     /locus_tag="VFN58_06750"
     CDS             complement(10569..11312)
                     /locus_tag="VFN58_06750"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET8917724.1"
                     /translation="MAQRIESLGGEVVEFPTIEIRPPESYGPLDQAINQIESYDWLIF
                     TSVNGVERFLNRFEKLGKNLANLAGIEVGAIGPETARRLTAAQIEPTLVPKQYQAEGI
                     LDELISETVLGKRILIPRAAKARDILPETLRRWGARVDVVEAYQTVLPQVDVSALCRL
                     LREGTIDMITFTSSSTATNFSVMLRDQDLPRLLSRAVIACIGPITKKTVEDLGMRPEI
                     VSEEFTIPGLVRAMVDYFSRTANERPAGR"
     gene            complement(11430..>11705)
                     /locus_tag="VFN58_06755"
     CDS             complement(11430..>11705)
                     /locus_tag="VFN58_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET8917725.1"
                     /translation="LSFFHDPATFAEVSAERSFLNRLGGGCHVPVGARGVAEGEKFKL
                     LGVVAKPDGSSLFRGETSGCAAHAEELGRELAERLLSQGAQQILIAR"
BASE COUNT         2845 a         3193 c         2975 g         2692 t
ORIGIN      
        1 agccccttcc cctttatgga acgccacagg taaattatct cttcttccgt gacgctttcg
       61 aaaatatcct gatcacgctc tctatctagt gctggaactg ggttaaatcg catagaaatt
      121 accaccgtcc tcatcacgat attgcgcaat agggaggtgc aaaccaaatg ccaactttgg
      181 aactgtttga aaaaccttag gttagcccct tctcttattc tcagacgccc ggtttttggg
      241 cgaatattgt caggatcgtc gaagttttat gacaatatat gtcctgaaat ggccatcaga
      301 actaatgaag gattgttcag tcaattacgg cgataccaga gatttcgacc atgcagtctc
      361 gattgtagag gctcaccacc tcaaccaacg tgatcgctgg aaagtattta ccgaacactt
      421 cgcgatacgt cccacccaac gcgtgcttgt tgttaatgta ggcttgcagg tctgtgatat
      481 agatcgtcag tacagcatat tggtcgggac gtccccccgc cgcttctact gccgtcttta
      541 gattctgtat aacctgggcg aactgcgtga cgaagtcccc cctgcccacg acttgaccgt
      601 gctcatcaaa aggcgcttga ccggcgacat aaacgatact tcctccagtc actttggcta
      661 catgtgtata gcccacggga cgttccagtc cttgggggtt caacagctcg aacggcatgg
      721 agtcaatctc cttttcgcat cagggtcagt tcagtcgcgg ccgcgctcca acaagtgatg
      781 aaatgcttca cttcgcttac cacgtcgtcg gatgcggcaa catatggaag aataatcctg
      841 gttgctcccg cttgctggta tggttttatc tgttcgacta tttcatccac tgacgttgcc
      901 gcgaccaggg gcaatccctc gaaaagccga tccgtgacga atttcctcgc cgcatgaaaa
      961 ccgcctgact tccaggccgc ccgcatcgcc tccacttccg cggcaaatcc catacggccc
     1021 aacagatctc tatagtaatc ggcgagggcg taataggtga gtgcatgact taacgcttcg
     1081 cgtgccgcct ggtggctcgg cgcgatagca caacggattt tgcagatgat ctctatcttc
     1141 gaaggatctt tacctgcctc ttcagcgccg cgcttaacct cttcggcaat tcgcctgatt
     1201 tcaatcggat tggccatgtt tattaataca ccatcggtca ttctgccagc caaacgggtc
     1261 attttggagc cgaatgccgc gagatagatg gggatggttc gccccgacgg acgaaaagcc
     1321 attttgaagt tttcggctgt aaaaaacttg ccataaaaat tgagcttgtc accccgcatc
     1381 gcagcgcgaa caatttctat gtattcctcg actctgccca atgggtgatc gaaggtttgc
     1441 ccatgccact ttgcgatggt cggattagat gacccgatac ccagaaaaaa acgtcccgac
     1501 gaaagctcgt caagtcccac cgcctgaagc gccaaagtag ccggcgttct ccctagaatg
     1561 tgatagaccg ccgagcctac tttgaggcgg gtagtgaccg cagcgatggc agccagcatg
     1621 accgtgggat ccttgttatt tgcctcgccg ccccaggcgc aatcaaatcc atgtttctcc
     1681 gcaagctgag cgagctctgg aatggcggcc atcggtaatt gggcaccgga attgaattcg
     1741 atatcgatct tcatgaatct cggtgtgtca acctggcgca aacatcaggt gtaactgctt
     1801 ggcaagttgg tttttagaat cgctattttt tcagtttttt ccacatgcct gatgaatcga
     1861 tatttgttct cgacttcatc gatcggatag ggcagcactg gcgtacgttc cgtgctaact
     1921 ttggcgccga tgaaaaaaag ctcatggccg ttcaaagcgt cagtggcgac tttggcaaac
     1981 tcatcgacgg aatgcgccca tagcgcctgt gcgatccccg cggcgcgcgc cattccaacg
     2041 agatccgtgc cgacagccgt cgccgttttt ttactgcctg aagcctcgta caccccattg
     2101 tcaaacacaa tgtgaaccag gtttttcggt tgcatgcgag caagggtcgg caacgaacac
     2161 aggttcatta acaacgatcc atcgccgtcg agcgcgataa cttttcgatg aggcaagcct
     2221 aatgccatcc caagagcgat ggaggagcaa agccccaaag tgcgcacccg aaaattaccg
     2281 tcgtcgggtc gtagggcatt ccattccaac gtcaccgctc cagcggaggt tacgaccaga
     2341 gcatccgtgg ccgccacagc aacagttttt aaacagtcga atctcttcat ctgtggttca
     2401 gtggattaag ctcgcgcatc ggcgttggca ttctatctaa agggacttga gcgaagctcg
     2461 acgcccgagt agttccttcg tcaatagcag cgctacagga cgcctggaat cctcagccag
     2521 aatctgtgcc cgtttgatca gctcgggagc gtcaccgggt tcgcgcataa tgtagtgccg
     2581 aatgcccaaa gcctggagca cgggctccgt cacactgccg gaagtactga acgtgcgatc
     2641 gccaatgtca cccgcgtagt aaatgagaag tagcaacggg atctggtatt gcaatagggt
     2701 cgttacaatg gcgttattgc tgttgaagaa accgccgttc tgcataatcg cggcgcactt
     2761 ctttcctacc aaataggcgc cggcgcagat ccctatcccc tcctcctcgc ggcacaaagg
     2821 aacgtgaatc acgtcgcgat ccgcttcaac ttcatgaagt aaatgggcga gattgatatc
     2881 aggtaaagtg gcgaccaggt tgatgccggc cgccttcaag ccgttaaaaa tggtttttgc
     2941 cgcgtcttcc tcagtcactg tcgtttccct atctgaacga gtaactcgct taacaccaaa
     3001 ccgatggtag atcaagcgtg cattcaccgc acgcagcact tttcctttgc tgtgaatctt
     3061 agtataaggt tcgtgatgaa caattgctga atatcgatat cgatttagaa aggagacgaa
     3121 caatgatcaa gctctatgac tttaaatcct cacccaattg ccagcgcgta aaggtcgtcc
     3181 tggcggaaaa aaatttaccc tacgaaatcg tgcccattga cctgaggaag caggaacaaa
     3241 aaactccgga gtacctcaag ctcaatcccg acggaaaagt tccagtgctc gtcgatgacg
     3301 ggacggttct ctacgagtcc tgcatcatca acgagtacct gaacgaaaag tatcccaatc
     3361 ctcctttgat gccggcggat ctcggcaaac gcgccaaggc ccgcatctta accgactacg
     3421 gactggctcg cctcgacgga ccgtatcaaa agctgcgcgt agaagtgacg aaggacccga
     3481 aggaacagaa tcagcaagtc atcgacacgg cgaagagcga tctccgaaaa ctcttgcagc
     3541 gccttgaaga tgcgattggc gatcagccct atttggtggg tgatttctcg ctggcggacg
     3601 cggacctcct gccccgtttc acaagattgg agggcttcgg cgtccttccc gacgcctctt
     3661 taccacggct gggaaaatac ctacagagaa tgaaagagcg gccctcagtg aaggccattc
     3721 tttagacgtt ggcgagtagg cattttccgt cccaccactt gtctttgcgc tagctaatgg
     3781 agacgccgaa gagcggcttg attccaaccg tcttttcggc tctccttcgc cctttcattt
     3841 gcttcctcga cagaggttcc ttcccgaagt atagggggag cctgcagctt gctgggctaa
     3901 gagacaaagt caaagtatgc tggggagctt acggcatccc tcacgcctac gccggcaatg
     3961 aggaagattt attcctcgct caaggctatt tgcacgccca ggagcgactc tggcagatgg
     4021 atatgagccg gcgcttcctc tccggacgct tggccgagat attcggcaac ttcccagtgc
     4081 cttggaaaga gttgtccagc cagtttcgcg gacaaaacag caccgatttc gattacttca
     4141 tgaggctcat gggcatccaa cactcggccg tggcatctct tcagcttgcc tccgaagatg
     4201 agcatcgacg actccatgct tacagccaag gcgtcaatcg atacatcgaa cagtgcggta
     4261 ggagaccgcc ctgggaattt cgcttgctcc gctatgatcc ggagccttgg cgaccggacg
     4321 atagtttaat catcggaaag ggctttgcat tccttctttc gacggctctc ttcacccgtc
     4381 tcaacatgat cgcgattgcc gcgaagctgg gcgatcatca agaaatgctg cgctccctgt
     4441 gtccaacata cccggatgat ggacccacaa taactcaagc cgtgtggaac tctgctcaaa
     4501 atatctggca attcatgaat ggcgcctatg ctcgtagtga ctggagtgcc gccggtcacg
     4561 gcagcaataa ttgggttgtc gccccgagtc gctcggccac cggccgcgct attctatgca
     4621 atgatccaca tttacgcctg acgattccat cgacttggta cttgatgcat ttgaaagccg
     4681 aaacatcgac gacccggcct gatggctacg aggtttgggg cgcctcaatc ccgggatctc
     4741 catgtgttca acttggttat aatcgttgga tttcttgggg agtaaccgca gcactctgcg
     4801 acgatgtcga actgtatagg gagaagccgc atcctctaga cccagaccgc tacctcacca
     4861 gagaccgctg gctcacgatg gcaaggcgcg aagaaatcat caaagtgcgg cgcagcggcg
     4921 aaacaaaaaa aatcgttcga tcaacttgcc atgggccggt gcttagcgat tttagcaacc
     4981 agttgaaccc gtcacctatt ctgtcgctcc gttggactgc ccacgagcca agccaggagt
     5041 ttcgctgttt gtatggcgtg aaccaagcac gcgattggcg cgaattcctt gacagccttg
     5101 cttatcacag tggaccgaca ttgaactacg tctacgctga ttccggcggc aacatcggtt
     5161 acagcctggc cggcagaatc cccttgcgac gagggattcc ctctttgctc ccgttagacg
     5221 gctggatcga agacaacgat tggctgggat acattccgtt cagtgagctg ccgcgcattt
     5281 acaatccacc tgagggagtg atcgcgacag ccaacaatcg catcgtcgat tcgtcttatc
     5341 cccattactt gtcccatttt tatgagccac cgtctcggat tcgccgaatc aaagaacttt
     5401 tggccgcgaa aaaatctttc tccatcagcg acatggagtc aatgcaaaac gatcgcgttt
     5461 ccctttatgc caccgagttg atcgcgacct tgaaaagcga tctggctcga gctccggaga
     5521 agagtggcca actcaggacg gctgctgatc gactcatcaa ctgggatggc aaatgcgatg
     5581 aagcaagcgt tgagtcggct atctttcacg tattccatta ccgcctgatg gcaaacttgc
     5641 tcgtccccac actgggggag gatctcttct cggcttatct ggaaattttc aaccaatcct
     5701 tgatgccaac ccatcaaatt ctgagagatc ccgactccag atggttctca acgacatcac
     5761 ggcaagacgt tgtggcaaag tctctccgag aggcgtgtgc agaattgaaa acaagcctcg
     5821 gcgacgaagt ggaactttgg caatggggaa aaattcacag cctgatgcta agccacgctt
     5881 taggtcacat caaactgctg cggccattgt tgaaccttgg tccgtttcca tcccctggcg
     5941 acggtacaac gatcaacacc ggtttttacc ggcattcaag cccctacgcc cacaccgtgg
     6001 gtgcttcgct acggttcatc atcgatgtcg gcgagtggca acagtcaggt tttgttcttg
     6061 cgtccggcca atccggccgc ctcttttcac cccattacgg cgatcagacg gcgctttggg
     6121 gcgcggggcg ttaccttaag attggaaccc aggacgagca aacgccattc gaaagtggat
     6181 tatttttggc gccaccctcg aagccacttc cttgactgct atcttaggaa accatataag
     6241 aaaccgtctc cccgaacatc ctggaatagc tgaagcggag gcattttatg tcgcttaaac
     6301 ttttaatcgc cgatgacgaa cggctgttca ggcaaagcct taaaatactc cttgagaccg
     6361 ccaaagatat aaaagtagtc gccgaggccg cggatggcca ggaggcagtg gtcaaatgcc
     6421 gggaaaccga accggacatc gctctgttgg atgttcgaat gcccaaaatg gacgggatca
     6481 aggccgccaa gctcatcgcc gcattgactc cccgaacgaa aattctcatg ctctccatcc
     6541 aggacgatga tgaaaagatc gcctcagcaa tcaaagccgg agcgaaggga tacatcctta
     6601 aagacaccga ccgagcggaa tttatcgaaa tcgtccgtcg ggcacacact gggaaaatcc
     6661 aaccttcccc ctatctcgct cagctcacgc tcgaacgtcc ttcggatgat gaagggccgc
     6721 ccaataatag tcgagaacga caattcagtg agaaatataa gcttacagat cttgaactca
     6781 aggttctcca attagttgcg gagggactca acaacgagga gatcagtcac gttacccacg
     6841 tggcccgcga gaccatcaag ggttacctca agggactgtt ccgcaagctt caggtcaaaa
     6901 accgcaccga ggccgccgtt ctggcagtga gggaggggat cgattgatga gcctcggatg
     6961 gggggactct aaaaaccccc aaatggggga ttgacattga ggtccgaagt aagagataag
     7021 gacggaaatt tttatcaact gaatagtttc cgagaacttt cgtctcaaag gaagaaggag
     7081 aagatagtat gaagctggcc aaaacgactt tgctaagttt attgctcgtt ttcgttcttt
     7141 tagccatgac tggcttcgcc gtggccctcg aagtaggtca aaaggccccc gactttgaac
     7201 tggagagcac caaaggcgga aagctgagct tgagcagcct taaaggaaaa aatgtactcg
     7261 ttaatttcta cgttctcgat ttcagtccga catgaattaa ggacttgcaa gcgtccgggt
     7321 cggacaatta tccgatgttc caggccgaaa atactgaagt tgtaggtatt agcgccaacg
     7381 cccctttttc acagaaagca ttcgctgact tcgcgaagat caactatccg cttttgagtg
     7441 accgagacgg caaagttatg caagcctttg gtgtttatga cgaaccgaga agaatcgcga
     7501 aacgggccta cgtaatcatt gacaaagacg gggtggttcg ctatttgaat atccgaccca
     7561 ccaacactga aaaggatctt ctctctacgg aacagttgtt aaacgaagta aaaaaagtca
     7621 ataaagggag ctaagaacta cgtctcaaag agaaaggaga aaacaactaa tggttcgagg
     7681 caaatttctt atcgccgcag tgatcggttt catgacgctg ctcgcttctt cccacgtttt
     7741 tgctcaggcg gacgtgatcg agaagagaca agcggcaatg aaggccaata gcgcggacgc
     7801 caaggcaatc aaggccgccg tagaatcaaa agactatgcg accattgagg tcaaggctaa
     7861 ggatatcatg gcaaccgcag aaaagatccc ggctctcttt cccaaaggta gcacgacggg
     7921 aaaaacgaag gccaccgacg ctatctggga aaaatccgac gattttgcca aagccgccaa
     7981 gaatctggga aaggcagcga gcgagttggc tgcaacggct aaggccaaag atgatgcaga
     8041 gatcggcgtg aaggtgaagg cactgggcga tgcgtgtggc tcctgtcaca aggcgttccg
     8101 ggctgaaaag tacgcagagt aagtaatcta agtcacgatc ttataagact acctctcaga
     8161 aggcccttgc ttcggtgaag caggggcttt cttttttcag cctgctataa tagtttttta
     8221 atgaaacaaa ggctcttcgt ttaggaggaa tctttttaat gaaaagaccg gtatttttct
     8281 gttcaattgt ttatttcact tggcttttcg ttctaatttc gtctgcttcg gggcaactaa
     8341 cccgactcaa cgtcggctac agcgccgtaa gcgccgacca gctgcccgcg tgggtcgcca
     8401 aagacgccgg catcttcgcc agaaacgggc tggacgtcca gttgatcttc ttcaccggcg
     8461 gaaccaccgc aattcttgcc cttgtgtcgg gtgacgtccc gataacccaa gtttcgggcc
     8521 cgggtttggt aaatagcgcc ttggcgggat cggatgctgt ttttgtcgcc gccggcatca
     8581 tatcgctgaa ttatgttctg atgggtaaac cgggaatcaa gactgcggaa cagctcagag
     8641 gcgggacttt agccatcagc cgcttcgggt cggcgacgga ttcgattgca cgattcgctc
     8701 tcagaaagat cggcttgacc ccaggtaaag atgtaactat cgttcaagtc ggaagcgggc
     8761 cggagcgcct tagcgccgtc ctgacgggcc gggcaacggc ggcggttatc aatccgcctt
     8821 ccagttttat cgccgaaaaa cgagggctag ccgttgttgc tgatgtcgcg caaatgggcc
     8881 tggtttttca acacaccggt gttgccacga cgcgcagatt catcaaggaa catcccgata
     8941 cggtgcggcg ctatgtcagg gctcatgtcg aggcggtcca aaagatgtgg accgacagag
     9001 aggccacgat caaagcttta agcaaatata tgggcagcgg agtcgaccgt gagactcttg
     9061 agaagagtta cgagaacgtc atgacggaat cgttttaccc caaaaagcag tatccgagcc
     9121 tggagggtct gaagacggtc ctcgaggacg taagcgagcg cgatccaaga gcgaagacgg
     9181 ccaagccgga gcaattcgtt gactttagct tcatcaagga actcgatggg agcggcgtta
     9241 tcgacggtct ttacaaaaag aaatgaaatc gcctaccggg atctcgtagt gataagcact
     9301 aaactaggcg aggacgcatc gtcggatatt caagactggg ccaatcgcgt cgccggcctt
     9361 gtggatagtg cggtaaccgt agatgtttac tacgacggcg actcaaatac ttacatctta
     9421 aggctggcga aagcggcccg cgtcttggtc tttcgcctgt ccgaatctca ggtgagtagc
     9481 tcaggacgag agacagaatg cgaacggacg ctccagcgta aggtcaaaga cctctggaat
     9541 ctgatctaag tgaataggga cgggttgaaa cccgccctta cttgccagcg aaaagccgcg
     9601 ccacatcttt cgcaaagtaa ctcaagatca tatccgcgcc cgcgcgtctg atggcgaaca
     9661 gtacttccat catgattctc tcttcatcga tccagccgtt ccgcccggct gctttgatca
     9721 ttgagtactc cccgctcacg ttataggccg ccacaggata accaaacttc tgtttcacac
     9781 gatagacgat atcgaggtag gccatcgccg gcttcaccat gatgatatcc gcgccttcac
     9841 ggatatcgag ctccacctcg cgcagggctt cgtccgcgtt ggcaggatcc atctggtagg
     9901 atcggcgatc accgaactgc ggtgtcgatt ctgcggcttc ccggaacggg ccataaaatc
     9961 ctgaggcgta ctttgccgca taggccatga tggcaatttg ttcaaagccg ttttcgtcga
    10021 gggattttcg aatcgctccc accctaccgt ccatcatatc ggagggcgcc acgatatcgg
    10081 cgccggcgcg cgcatgcgac aacgcttctt tcgcgagcag ctccagcgtg ctgtcattgt
    10141 cgacgtcgcc gtttttgatt acgccgcagt ggccatggtc ggtgtactcg cacaaacaga
    10201 cgtcggtcat gatcagaagt ccgggaacct tttctttgac agcgcggatc gcctgctgca
    10261 ccacgccggt gtccgcataa gcctccgtgc cgacggaatc tttgtgctcg ggaacgccga
    10321 aaagaataat cgcagggacg cctagagcct gtgcctcttc acactccgta acagccctat
    10381 ccacagagag ttgtgagacc ccgggcattg aagacaccgg ttgagcccta ttcttccccg
    10441 gcccgacaaa aatcggataa atgaaattct tgggactgag cgtggtttcc cgcaccagct
    10501 ctctgatctt ctcgttacgg cgcaatctcc tcgggcggta agcgggaaag tccattattc
    10561 tgtcgccctt atctaccagc gggtctttca ttcgcggtac gggaaaaata atccaccatg
    10621 gccctaacca acccaggaat tgtgaactct tctgagacaa tctccggccg catgcctaag
    10681 tcttccacag tttttttagt aattggaccg atacacgcaa taaccgctcg agaaagcagc
    10741 cgtggcaggt cctgatcgcg tagcatgact gagaagttcg ttgccgtgct ggagctcgtg
    10801 aaagtaatca tatcaatggt gccttcacgc aatagccggc atagcgccga aacatccacc
    10861 tgaggcaata cggtttggta tgcctctacg acatcaaccc gggcccccca acggcgcaga
    10921 gtttccggca ggatgtcacg cgctttagct gcccgtggaa tcaatattct tttccctagc
    10981 acggtttcgg aaatcagctc gtccagaata ccctcagcct ggtattgttt cggcacgagg
    11041 gtgggctcaa tttgcgccgc cgttagcctt cttgccgtct caggaccaat ggcaccgacc
    11101 tcgataccgg ctaggttggc taggtttttt ccaagctttt cgaagcggtt aagaaatcgt
    11161 tcgacgccat tgacgctcgt gaaaatgagc cagtcgtaac tctctatctg gttgatcgcc
    11221 tggtccaaag ggccataact ttcgggcggt ctgatttcta tcgtgggaaa ttcaaccact
    11281 tcaccgccca agctctcgat cctctgcgcc aagctcaacg cctgagatcg ggcgcgcgta
    11341 actaggatac gcttccccct aagagcatcg gatgaaaccg gctgttcatc tggtattcgg
    11401 atagccatag tccgaagcca caacctcttc tagcgggcaa tcaaaatctg ttgtgcaccc
    11461 tgggacaaca ggcgctcagc gagctcacgg cccaattcct ctgcgtgcgc tgcacagccg
    11521 gatgtttcac cgcgaaaaag ggaacttcca tccggcttgg cgacaacgcc gaggagctta
    11581 aatttttcgc cttccgcaac ccctcgcgcc cccactggaa cgtggcaacc accgcccagt
    11641 cgattcaaga aggagcgttc cgcgctgact tcggcaaaag tagccggatc atggaagaac
    11701 gaaag
//
LOCUS       DASSWZ010000277         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_449061, whole genome shotgun sequence.
ACCESSION   DASSWZ010000277 DASSWZ010000000
VERSION     DASSWZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449061"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFN58_06760"
     CDS             complement(<1..576)
                     /locus_tag="VFN58_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET8917726.1"
                     /translation="MIKQYLLSPGPTPIPNEVALAMSETMIHHRTPQFNKVFEEARQG
                     LKALFGTKGDVLILASSGTGAMEAAVSNLFSPGDKVLVINGGKFGERWLNIANAFALD
                     PIEVKVEWGQAVKVDVVEKQLKLHPDIQSVMLQASETSTTVLHPVKEIANLTKNGPLF
                     LVDGVTAVGVLPVPLDKWGIDALVTGSQKALM"
     regulatory      complement(704..788)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            895..1590
                     /locus_tag="VFN58_06765"
     CDS             895..1590
                     /locus_tag="VFN58_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET8917727.1"
                     /translation="MIELLILFDLDGTLTRTQKGYVPFNEAIRETFGVVGDIRSVIPD
                     GNTDPQIVREIFAKANVEKEIDDGQWEQFAKNLRRSYSRALRGATTTVRALPGAPELL
                     SLLSEKAELSQGVVTGNFEVTAQIKLEAAGLHSYLCRGAYASDSAHRPDLPRIAKERW
                     EQSTGKSLRAEQCVIVGDTPKDLDAARQNHMKCILVGTGRYPWEELQYWKPDACLPDL
                     TDTLEIVRTLSNL"
     gene            1614..2618
                     /locus_tag="VFN58_06770"
     CDS             1614..2618
                     /locus_tag="VFN58_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8917728.1"
                     /translation="MQYRTIGQTGVKISEIGFGCGNNAVLMVKAPYEDQRKAVRHALD
                     RGINFFDTAFAYGLGKSEENLGRILNDLGAQPVISTKIRLEADAISDVGAATIRAVEA
                     GLKRLNRDRVDFIQLHTRVTLERGAGKRFSLTPQDVLGPKGVVEGFKTMRNRGKVGYF
                     GFSGLGDVTALHQLVDSGEFHGFQAYYNLLNPSAGQEVPQGFGALDYRRIIDRAAAKG
                     MGAFVIRVLAAGALTSDPSPGGGSSAEPLSPGSDYPLDIERAAKVKAALGIDGRSLTQ
                     AAIRFGLMKSEVSTVLVGFSNTAHIDEAVACSGAGGLSQDAMAKMRQLWDSDFGQSDR
                     "
     gene            2664..3695
                     /locus_tag="VFN58_06775"
     CDS             2664..3695
                     /locus_tag="VFN58_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8917729.1"
                     /translation="MKKLFIAFFLVIATDELNAQSSFYQGKTIRIIVGYLPGDNHDLW
                     ARAYGRFMGKHIPGNPDFVVQNMPGAGSMIAANHVYNLTKPDGLTLGAIGGALYYAQL
                     TGRGEVKFDWAKFTWIGTADRNGTLVFMRSDAPYKTLEDIRNAKEGPKCSATGVGSAG
                     YDMPRLLGETLGLKFTVISGYPGGAEQDLALERGEVQCRAITVDGFFGREPFLSWYKT
                     GFVRVLLQTGRQRNARIPDVPTLYELMDQYKIPDAKKRLATVYLGAGGFGSRPIVATP
                     GVSAERAQILREAYAKTLKDSEFLEEAKKKRWPEIQFSSGAELEALAKEVIDQPPEVI
                     DWLKNLLGK"
BASE COUNT          870 a          955 c         1119 g          891 t
ORIGIN      
        1 catgagcgct ttttgcgaac ctgtgacgag cgcatcgatg ccccatttat cgagggggac
       61 gggaagcact cccacggctg tgacaccatc gaccagaaac agcggcccgt tcttcgttag
      121 gttggcaatt tcctttaccg gatggagcac ggtggttgat gtctcgctcg cctggagcat
      181 gacactttgg atgtccgggt gtagtttgag ctgtttctcg actacgtcaa ctttgaccgc
      241 ctggccccat tcgactttga cctcgatcgg gtccagcgcg aacgcgttgg caatatttaa
      301 ccagcgctcg ccaaacttac cgccattgat cactaggacc ttgtcgcccg gcgagaacag
      361 attcgatacc gccgcttcca ttgctcccgt gccggaggag gccagtatta acacatcacc
      421 tttggttccg aagagggctt tgagaccttg gcgtgcttcc tcgaatacct tgttaaactg
      481 aggcgttcga tggtgaatca tcgtctccga catggccaat gcaacctcat tggggatcgg
      541 ggtgggaccg ggtgagagga gatattgctt gatcatgaat gtccttttta cctatcggcc
      601 agttggagct ggactgtgct gcgccgaggc aggcgaaggg tgtgataaac ggcgtcagaa
      661 gtcaacggca ttgcatccat aaaaaaagcc tgaacctctt ctattaagag acccaggcgt
      721 tcggccgtct taatgctttg cactccctcg tggtcgattc cacgctgacg ccagtcgcgc
      781 aaaaccttag ggaaaacttg tatcttagaa gcatgcgctt gtcaatggtt tggtattgct
      841 acgagggttc gtcagtttgc caaggtcaac gtgattgtgc aaatatccgc atgcttgata
      901 gaactactta ttttatttga tctcgacggc acgctaaccc gtacgcagaa gggttacgtt
      961 cctttcaacg aagcgattcg cgagacgttc ggtgtggtcg gtgacatccg atcggtgatt
     1021 cccgacggca acacggaccc gcaaatcgtt cgggaaatct ttgctaaagc aaacgttgaa
     1081 aaggaaattg atgacggcca gtgggagcaa tttgcaaaga acttacggcg gagctactcc
     1141 agggcgctgc gcggggcgac gacaacggtt cgtgctcttc ccggcgctcc ggaattgctt
     1201 tcgttacttt cagaaaaagc ggagttgagt cagggcgtgg ttaccggaaa tttcgaggtg
     1261 accgcccaaa ttaaacttga agcggcggga ttgcattctt atctctgccg cggtgcttat
     1321 gccagcgatt cagcccatcg ccctgacttg cccaggattg ccaaagagcg ttgggagcag
     1381 tcgacgggaa agtctcttcg cgctgaacag tgcgtgatcg tcggtgatac cccgaaggat
     1441 ctcgatgctg ccagacaaaa tcatatgaag tgtatcttgg tggggacggg aaggtatccg
     1501 tgggaagagc tccagtactg gaagccggac gcttgcttgc ctgatctcac tgatacgctg
     1561 gaaatagtga gaacactttc aaatctctag tatatccgag atgaggtgac acgatgcagt
     1621 atcgaacgat tggtcaaaca ggtgtaaaaa tttccgagat cggcttcggc tgcggtaaca
     1681 atgccgttct catggtgaag gcgccttacg aagatcaacg gaaggccgtc cgccacgcgc
     1741 tcgatcgagg aatcaatttc tttgatacgg ccttcgccta tggcttgggc aagtccgagg
     1801 agaatctagg acggatactc aatgacctcg gcgcacaacc cgtgatctcc accaaaattc
     1861 gattagaggc tgatgccatc agcgatgtcg gagccgctac gatccgggcg gtggaagcgg
     1921 gactcaaacg actcaataga gatcgcgttg actttatcca gcttcacacc cgagtcacgt
     1981 tggagcgggg cgcggggaag cgcttcagtc tgacgccgca agatgtcctc ggacccaaag
     2041 gggtggtcga aggcttcaaa acgatgcgga atcgaggcaa ggtgggctac ttcggcttta
     2101 gcggattggg tgatgtgacg gcgctgcacc agttggttga cagtggggag ttccatgggt
     2161 ttcaggccta ctataattta ctcaacccga gcgctggcca agaggttccg caaggcttcg
     2221 gcgcgttgga ttaccgtcgt attatcgatc gggcggcggc caagggaatg ggcgcctttg
     2281 tcattcgcgt tttggcggcc ggcgcgctga catcggatcc cagtcccggc ggaggcagca
     2341 gtgccgaacc cctatctccc ggatccgact atcctttgga tattgagcgt gccgcgaagg
     2401 tgaaagctgc gctcggtatt gatggaagga gtctgacgca agcggcaatc cgctttggtt
     2461 taatgaaatc ggaagtctcg actgtactcg tgggcttttc caacacggcc cacatcgatg
     2521 aagcggtcgc ctgctccggg gccggcggat tgtcccagga cgcgatggcg aagatgaggc
     2581 agctttggga tagcgacttc ggtcaatcgg accgttaagt gaggttacta ccggtgttga
     2641 cgcaagcatg aaaggaggag ccaatgaaaa agttgttcat cgcttttttc cttgtcatcg
     2701 caaccgatga attgaacgcg caaagctcct tctatcaggg taaaaccatc aggattattg
     2761 tcggttatct gcccggtgat aatcacgatc tgtgggctcg cgcctatggc cgctttatgg
     2821 gaaagcatat tccggggaac cccgacttcg ttgtccagaa catgccggga gcggggtcca
     2881 tgatcgcagc taatcatgtc tataacttga ccaaacctga tggattgacc ctcggcgcca
     2941 tcggtggggc gctgtattac gcccagctca caggccgcgg ggaggtcaag ttcgactggg
     3001 cgaagttcac ctggattgga acggccgatc gtaacggaac cttggtcttc atgcgctctg
     3061 atgctcctta caagacgctt gaagatattc gcaatgctaa ggaggggccg aagtgttccg
     3121 ctacaggagt cggcagcgcc ggttacgata tgcccaggct gctgggagaa acgctgggat
     3181 tgaaattcac tgtgatcagc ggttatccgg gaggggcgga gcaggactta gcgcttgaaa
     3241 ggggagaggt ccagtgccgg gcgataaccg tcgatggctt ttttgggcgc gagccgtttc
     3301 ttagttggta taagaccggt ttcgtccggg ttctcctgca aaccggtcgc caacgtaacg
     3361 caagaattcc ggacgttccc actttgtatg agctgatgga ccaatacaag ataccggatg
     3421 cgaagaagcg actggcgacg gtctatttgg gcgcgggcgg ttttggctcg cggcccatcg
     3481 tagctacgcc aggcgtctcc gcagagcgcg cgcaaattct ccgtgaggct tatgctaaga
     3541 cactcaaaga ctccgagttc ttagaggaag caaagaaaaa acgctggccc gagatacaat
     3601 tttccagcgg cgcagagtta gaggctttgg caaaagaggt gatcgatcag cctcccgagg
     3661 tgatcgattg gttgaagaac cttttgggga agtaactgca gattttttgc tcagcaggag
     3721 gttaaaatga gaaagctcgc gttagcaatc tttcttttcc taatctgtaa tccgcctgtt
     3781 caagctcaaa cgccttacca tcaggggaaa accgtgacaa ttgttgtggg gacca
//
LOCUS       DASSWZ010000278         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_534488, whole genome shotgun sequence.
ACCESSION   DASSWZ010000278 DASSWZ010000000
VERSION     DASSWZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534488"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFN58_06780"
     CDS             complement(<1..239)
                     /locus_tag="VFN58_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917730.1"
                     /translation="MDLSALQSERVEVADDLWAVNAFFEVKGWTDGLPIIPPTEERVG
                     QMIAAVKRNPQDVIGVVPPRWAPATVEKIAINAVMA"
     gene            complement(337..531)
                     /locus_tag="VFN58_06785"
     CDS             complement(337..531)
                     /locus_tag="VFN58_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917731.1"
                     /translation="MTLITDAFDALAKSEKEAFGAPDHPVLVVQHPIGTVKVEEVNKR
                     ADAAFDQLVELLVKPEADKK"
     gene            complement(595..879)
                     /locus_tag="VFN58_06790"
     CDS             complement(595..879)
                     /locus_tag="VFN58_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917732.1"
                     /translation="MIVLSPEGKARTSGARVAALPRFTELNGKVVGLLDNSKPNADKL
                     EERFAQLLRERLDVAKVVTRRKISAQQGAPKEYLDELASQADFILSGLGD"
     gene            1036..1404
                     /locus_tag="VFN58_06795"
     CDS             1036..1404
                     /locus_tag="VFN58_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HET8917733.1"
                     /translation="MRKSFWPGVLIFLLVSVILATVGSVFAHHGWSGYDSSKALELKG
                     TIVESGYEHPHGFVRLKAADKTWFVVLAPPSRMERRQLPRKNLASGTEATVIGYPSRT
                     DLQEMRAERITINGRTTELR"
     gene            1463..1933
                     /locus_tag="VFN58_06800"
     CDS             1463..1933
                     /locus_tag="VFN58_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004880668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HET8917734.1"
                     /translation="MLVWLETTSLASAMREWLWLYPIVEILHIIGLAVLVGAAVLFDL
                     RLLGVSRILPVTALAAHVLPWARASLLFIVPSGLMMFTAHATEFATNPAFQLKLALLA
                     AASANAIAFHLRTFRTVAAWDRGTPAPPAAKVAAILSLLLWSGVIICGRLLAYL"
     gene            2108..3088
                     /locus_tag="VFN58_06805"
     CDS             2108..3088
                     /locus_tag="VFN58_06805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8917735.1"
                     /translation="MEYRQFGTTEFKVSPMGVGCMSMSGAYGPADDAESIATLHRAFD
                     LGINFLDTSASYGSGHNHELIAKALKGRRERIVIHSKSGSPRSPDASGNRSGSTPEYL
                     TQNCEQSVKRLNIDCLDIFCMSRVDPKVPVEESVGAMARLVEQGKTRYIGLSEASANS
                     IRRARKVHPIVSLQMEYSLWSRDPEGGNIQACRDFGMGFMAYSPLGRGFFAGAVHNPK
                     DLAEGDGRSNHPRFQQENLEHNLKLLDEFEQIAKEKSATPAQLALAWLMAQGKDIIPI
                     PSNKSRRHLEENLKAIDIKLTQDDITQLDKLFKPGAAAGPRTRDMDRVNV"
     gene            complement(3152..4234)
                     /locus_tag="VFN58_06810"
     CDS             complement(3152..4234)
                     /locus_tag="VFN58_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917736.1"
                     /translation="MPIIDADTHIDETEDTWEYMQESDLQFKPTTAYPPNRNPEMLPT
                     RYWVIDGKRQLRFIRGDEESGTVVESRELLDVQVRLKHMDELGTDIQVIYPTLFLMEA
                     TERQEVSTALRRSYNRWLADRCGQSNGRLRWICLPPLQNMDETLKELRFAKEHGACGA
                     LKKGDREAGKWPADPYFFPFYEEAERLDLPICFHTGSGIPDFSPAREFTLGQFMRTKA
                     AAVNGIETILVNNIPAKYPKLRFGCIEAGASWVSFVDYDLRRRVQSRANRVSILGGPN
                     TILASNLFKNNRVYITCQVDEDLPYILKYVGEDNLIVGSDYTHRDPSMELEFRSLLQQ
                     RADEGEIPKSAVQKILYDNPKAFYGL"
BASE COUNT          937 a         1231 c         1152 g         1003 t
ORIGIN      
        1 gccatgaccg cgttgatagc gattttttca acggtggccg gcgcccaacg aggtggcaca
       61 acgccgatca cgtcttgcgg atttcgtttg accgccgcga tcatctggcc cacccgctct
      121 tccgttggag gaatgatcgg caagccatcc gtccaacctt tcacttcgaa gaaggcattg
      181 acggcccaga ggtcgtcggc aacttctaca cgttcagatt gcaatgcact taagtccatt
      241 tttgttctcg gttcgaggtt caatgttcag gttacgagat gcggtatttg gtaaagcttc
      301 ctgacacctg aaacctgttc tcgtcggtcg gccggcttac tttttgtctg cctcaggttt
      361 gaccaaaagc tccaccagct gatcgaaagc agcgtcggcg cgtttgttga cctcttccac
      421 tttcacggtg ccgatcggat gctgaacgac cagcaccggg tgatccggcg cgccaaatgc
      481 ttctttctcc gatttggcca aagcgtcgaa ggcgtcggtg attaaagtca cggcaaaccg
      541 gccgcgcttt tgtaattcta ttgagtcgtg gatactccac gaggtgcacg accctcaatc
      601 cccaaggccg ctgaggataa aatcggcttg agatgcgagc tcgtccaaat actctttggg
      661 cgcgccctgc tgcgcgctaa tcttgcgccg tgtgacaacc ttagcgacat ctaatcgctc
      721 tcgcagcaat tgcgcgaacc gttcttcaag cttgtctgcg ttgggcttgc tgttgtccag
      781 cagcccaaca acttttccat ttagctctgt gaatcgcggc agggctgcca ctcgggcgcc
      841 agaagttcgt gcttttcctt cgggactcaa gacaatcatt tattgtcctc cttgcaacgc
      901 ccggactata aatcggtcgg atgctcgtgt caaagaggca gttggatttg caaggcctcc
      961 taatagccat cttgagtcgt cctgagctgc catctatatt ctctagcagt gaattgaaag
     1021 gagttgataa gcgccatgag aaaaagcttt tggcccggtg tgttaatctt cctactggtt
     1081 tcagtgattt tagcgacagt cggctcagta ttcgcccatc atggatggag cggctatgac
     1141 tcgagcaagg ctcttgaact caaaggaacc atcgtcgagt cggggtacga acatccgcat
     1201 ggttttgtcc ggctcaaggc ggcggacaaa acttggttcg tcgtgctggc tcccccttca
     1261 cggatggaaa gaaggcagtt gccacggaag aaccttgcat caggcacgga ggctaccgtg
     1321 atcggttatc ccagccgcac cgaccttcag gagatgcgcg cggagcgcat cacgattaac
     1381 ggccggacga cggagctccg ataatatctt ggttgcaatt cctcttagcg cgtaaccaat
     1441 gcatccaccc tcaggacccg cattgcttgt ctggctcgag acgacaagcc tggcatctgc
     1501 gatgcgcgag tggctgtggc tttacccgat cgtggaaatt ttgcacatca tcgggcttgc
     1561 cgtcctcgtg ggcgctgccg ttttattcga tctcaggctg ctcggagttt cgcgcatcct
     1621 gccggtgaca gctctggcgg cacatgttct tccctgggca cgggccagct tgctttttat
     1681 tgtaccgtcg ggactgatga tgttcactgc ccacgccacc gaatttgcga caaaccccgc
     1741 ctttcagtta aaacttgctc tattggcggc cgcgtcggct aatgcgatag catttcacct
     1801 ccgcaccttt cgaacggtag ccgcttggga tcgtggcacg cccgcgcctc ccgcagccaa
     1861 ggttgccgcg atcctatcgc tgttgctctg gagcggcgtg attatctgcg gccgtctgct
     1921 ggcgtatctc tgaatacaac cgcatctctg tcctctccac ttcgacaagc tcagtgtggt
     1981 gagccccagt cgaaccaccg tcgagaggat ggtagtaggg tcggttgaaa ttgtacaaac
     2041 attttgtttg tgactgatcg atacatctgc cataactgtg gccggaagat agattggagg
     2101 caacgcaatg gagtatcgac aatttggaac cacggaattc aaagtttcgc ccatgggcgt
     2161 cggctgcatg agcatgtccg gcgcgtacgg accggccgac gatgccgagt ccatcgccac
     2221 cttgcaccgc gctttcgatc taggaattaa tttcctggac acgtcggcca gttacggcag
     2281 cggccacaac cacgaactga tcgccaaggc gctcaagggc aggcgcgagc ggattgtcat
     2341 tcattccaag tccgggagcc cgcgcagccc cgatgccagc ggcaatcgca gcggcagcac
     2401 acccgaatac ctgacgcaaa attgcgagca aagcgtcaag cggctcaaca tcgactgtct
     2461 cgacatattc tgcatgtcac gggtcgatcc gaaagtgccg gtggaagaat ccgtcggtgc
     2521 catggcccgc ctggtggaac agggcaagac ccgttacatc ggcctatcgg aagccagcgc
     2581 aaactcgatc cgccgcgcgc gaaaagtgca tcccatcgtc tccctacaga tggaatactc
     2641 gctttggtct cgcgaccccg agggtggcaa tattcaagct tgccgcgact tcggcatggg
     2701 tttcatggcc tactcgccgt tgggacgtgg cttcttcgcc ggcgccgtgc acaatcccaa
     2761 agacctggct gaaggcgacg ggcgcagcaa ccatccgcgt tttcagcagg aaaacctcga
     2821 gcataactta aaactgcttg acgagttcga acaaatagca aaggaaaagt cagcgacgcc
     2881 tgcgcaactg gcgctcgctt ggctgatggc ccaaggtaaa gacatcatcc cgattccaag
     2941 caacaaaagt cgccgtcacc tggaggaaaa cctgaaggcc atcgatatca aactgaccca
     3001 agacgatatc acccagctcg ataagctctt caaacccggc gctgccgccg gcccgcgcac
     3061 tcgcgatatg gaccgggtga atgtttaaag aagaacgggc gggtttaaag cccgccccta
     3121 catggcatcc ttcgtggcga aatttttccg atcacaggcc gtagaacgcc ttggggttgt
     3181 cgtaaagaat cttctggacc gcggatttcg ggatctcccc ctcatcggcc cgttgctgta
     3241 acaaacttct aaactccagc tccatagatg ggtcgcggtg cgtgtaatcg gagccgacga
     3301 tcaagttatc ctcccccaca tatttaagga tgtaaggcag gtcttcatcg acctggcacg
     3361 taatgtaaac acgattgttc ttgaacaggt tgctcgccag gatagtattt gggccaccaa
     3421 gtatgctgac acggttggca cgcgattgga cacgtctacg aaggtcatag tcaacaaacg
     3481 acacccacga cgcgccggct tcaatgcagc cgaaacgaag ctttgggtac ttggctggaa
     3541 tgttattcac gagtatcgtc tcgatgccgt tgacagcggc cgctttggtt cgcatgaact
     3601 gcccgagagt aaattcccgt gccggcgaga aatcggggat ccctgaacct gtatgaaagc
     3661 aaatgggcaa gtccagccgt tccgcttcct catagaaagg aaagaaataa ggatcagccg
     3721 gccattttcc ggcctcgcga tctccttttt tgagggcgcc acaagcgccg tgctccttgg
     3781 cgaagcgcaa ctctttgagc gtttcgtcca tgttctgcaa aggaggtagg caaatccagc
     3841 gcaagcgccc gttggattga ccgcagcgat cggcgagcca gcggttgtag ctgcgcctga
     3901 gtgccgttga aacctcctgg cgctccgtcg cctccatcag aaacagcgtt ggataaatga
     3961 cttggatatc ggtccccagc tcatccatat gtttgagccg aacctgcaca tccaatagtt
     4021 ctctcgattc caccaccgtg cccgactcct cgtcgccgcg aatgaaacgt agttggcgtt
     4081 ttccatcgat cacccagtag cgggttggca acatctctgg attgcggttg gggggatagg
     4141 ccgtggtcgg tttgaactgc aaatcggatt cttgcatgta ctcccaagtg tcctctgtct
     4201 catcaatgtg agtgtcggca tcgattatgg gcatttcaga tcctcctcct aatggagact
     4261 tagcctaccg cgcaaaaaat ttctacggtt ggtattgcgc ctcttcggcg aaatcggcta
     4321 acg
//
LOCUS       DASSWZ010000279         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_647658, whole genome shotgun sequence.
ACCESSION   DASSWZ010000279 DASSWZ010000000
VERSION     DASSWZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647658"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VFN58_06815"
     CDS             <1..1680
                     /locus_tag="VFN58_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET8917737.1"
                     /translation="SEDGLRSVKGMRDFLREADEKRREMLKKYSPDSFKLTPEEEKAL
                     SDKLSSLAEKLEAYHEQMRNFMERLSGKYAGNMDEMQRRMQEAYQRYQELRNRLQNQI
                     SDRSMQQQRQNLTGQNYQSLMDMLDRMNQLLQDENFLKNLPNMLDAAVDDLDNMLENL
                     DSMTQEQLQQLSDMMSQMQQLEQLLNQYPFQGSQRMGMGEAGQILGQLRGLERFLRWG
                     QRGMGDPSELDLEEIRQLLGEEAYEHLKYLKGVEQMLEEEGYIVRTNHGLKLTPRGIR
                     KIGDKALREIFQMLNKSRWGNHNTAVRGSQGDRLEETKNYEYGDPMNINISETLLNAL
                     EKRKKGAPLKIAPEDFSVHRVEFSTASETALLIDVSYSMLMNDALHAGKKVALALHRL
                     IETKYPQDTLHLVAFRSNAKLIKAEDLPSIVSLTYFMEHGTDIKEALRFARQLLGSHK
                     TTNRQIILITDGEPTAATLDRGGRLHSGWGSALLHSRIVHETLKEVKRCTQSGIKINT
                     FMLGADFYRQGFIDQLSRLNTGRVFYTSPDQMGNYIMVDYIANKRKRIGRA"
     gene            complement(1706..2884)
                     /locus_tag="VFN58_06820"
     CDS             complement(1706..2884)
                     /locus_tag="VFN58_06820"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET8917738.1"
                     /translation="MIRAGVGQSSNPSTERAAEQAALEAMAQAGISQADAVVVFFTAE
                     HAPHSKSLLSTLTRVTRSDRIVGSSGAGILTGAGEIEGRHGLAVLVFASDQMQSQPFL
                     FQPLREHDEEVGGRIAQMVGTDQSSLLALFPDTYNGQPHRLLRSMEQKIGFVPVVGAG
                     SSESGMTQATYQVCGEKCQSNSVAGLQLSGSFEAVIDITQGCQPITEPMVITKAEGNL
                     ISEINDRPALEVFAKLLKGPLAEDLRRALMFIFVGLPADPNQGTIGPGQYLVRNIIGL
                     DPAKGIVGIADQVREGQSIIFTLRDGQRAREDLNQMLQRQARKLDGKAPAFGLYFNCC
                     ARGNSLYGMPGIDTAYIRQALGDFPLLGTFGGYELAPLGRKNHLFAYTGVLTLITEKS
                     "
     gene            3025..3792
                     /gene="gloB"
                     /locus_tag="VFN58_06825"
     CDS             3025..3792
                     /gene="gloB"
                     /locus_tag="VFN58_06825"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HET8917739.1"
                     /translation="MKIVQIPLLRDNYGYLVVCQKTNTAGIVDPSEAEPVWRRVELEK
                     VTLTAILNTHHHRDHTGGNEGLLAKQSLEVYGHKSDQGRIPGLSRGVDEGDEIVIGEE
                     RGSVLFIPGHTTGHVAYLFGNNLFCGDTLFTAGCGRLFEGTAEQMHGSLKKLMGLPDD
                     TKIYCGHEYTESNLRFAMTLEPKNHKLVSRFERVQGLRARGASTVPSTMEEEKQTNPF
                     LRWDSKEIQARLKAGSSGLRLDPVSVFATVRKLKDAF"
     gene            3918..>4229
                     /locus_tag="VFN58_06830"
     CDS             3918..>4229
                     /locus_tag="VFN58_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HET8917740.1"
                     /translation="MNLKERTYLWYIALLRIYIGYYMLQQGIRKFQRDFPRGDWVGRQ
                     IGDLATIDLYPWYKKFLVDYVVPHQELFGYLVMLGEILVGGCLLLGLLTRLNAFVALF
                     ML"
BASE COUNT         1016 a         1188 c         1136 g          889 t
ORIGIN      
        1 tcggaagacg ggctccgctc ggtcaaaggc atgcgggatt ttctgcgcga ggccgatgag
       61 aagcggcgcg agatgctgaa aaaatattct cccgattctt tcaagctgac tccggaagaa
      121 gaaaaggccc tcagcgacaa actcagcagc ctggcggaaa aactcgaggc ctatcacgag
      181 caaatgcgga atttcatgga gcgtctctcg ggcaagtacg ccggcaacat ggacgaaatg
      241 cagcgccgga tgcaagaggc ttatcagcgc tatcaagaac tgagaaaccg gctgcaaaac
      301 cagatctccg atcgcagcat gcaacagcag cgccagaact tgacgggaca gaactatcag
      361 agcttgatgg acatgctcga tagaatgaac cagcttctgc aagacgagaa ctttctcaag
      421 aacctaccca acatgttgga cgcggccgtc gacgatctcg acaacatgct tgaaaatctc
      481 gactccatga cccaagaaca gctgcaacag ttgagcgaca tgatgagcca gatgcagcag
      541 ctggaacagc tcctgaatca gtacccgttt cagggctccc agcgcatggg catgggtgaa
      601 gcgggtcaga tccttgggca actgcgcggc ctggagcgtt ttctccgctg gggccagcgc
      661 ggcatgggcg atccttccga gctcgatctc gaggaaattc gccagctcct tggcgaagaa
      721 gcctacgagc acttgaaata tctcaaaggc gtcgagcaga tgcttgaaga agagggatac
      781 atcgtccgga cgaatcatgg cctcaaattg acgccgagag gcataaggaa gatcggcgac
      841 aaggctttgc gtgagatctt tcagatgctg aacaaaagcc gctggggcaa tcacaacacc
      901 gccgtgcgag ggtcgcaggg tgaccgcctc gaagaaacga aaaactacga gtacggcgac
      961 cccatgaata tcaatatcag tgaaaccctg ctgaacgccc ttgaaaaacg caagaaaggc
     1021 gccccgctca aaattgcgcc ggaggatttt tccgttcacc gtgtggagtt ttccaccgcc
     1081 agcgaaaccg ccctattgat tgacgtgagc tactcgatgt tgatgaacga cgcgctgcac
     1141 gccggcaaga aggtcgcgct ggctctgcat cggctcattg agaccaaata cccgcaggat
     1201 actttgcacc tggtcgcctt tcggtccaac gccaagctga tcaaggcgga agaccttcca
     1261 tcgatcgtct ctctcaccta cttcatggaa cacggcacgg acattaaaga agccctgcgt
     1321 ttcgcgcgcc agctcttggg ttctcacaaa accacgaacc ggcagatcat cctgatcacc
     1381 gacggcgagc ccaccgcggc aaccttggac cgcggcggcc gactccacag cggctggggc
     1441 tcggctttac tccacagccg gatcgtccac gagacgctca aggaagtgaa gcgctgcact
     1501 cagagcggga tcaaaatcaa tacttttatg ctcggcgccg acttttatcg gcagggcttt
     1561 atcgatcagc tttcccgtct caacaccggc cgcgtttttt acacgtcgcc ggatcagatg
     1621 ggtaactaca tcatggtgga ctatatcgcc aacaagcgga aacgaatcgg gcgcgcctaa
     1681 ccacccactc tcaccgcaat cccgttcaac tcttctcggt aatcaacgtc aggacgccgg
     1741 tataggcgaa aagatggttc tttcttccca gcggcgcaag ctcatagccg ccgaacgtgc
     1801 cgagcagcgg aaagtcgcct agcgcctgcc gaatgtaggc tgtatcaatg cccggcatgc
     1861 catagagcga gttgccgcgc gcacagcaat taaagtatag accgaacgcg ggcgcttttc
     1921 cgtctagttt ccgcgcctgc cgctgcagca tttgattcag atcctcccgc gctcgttggc
     1981 cgtcgcgcaa ggtaaaaatg atggactgtc cttctcgcac ctggtcggca atccccacga
     2041 tgcccttcgc gggatccaat ccgatgatgt tacgcacgag atattgcccc gggccgatag
     2101 tgccttgatt cgggtctgcc ggcagcccga cgaaaatgaa catcagtgcc cgcctgagat
     2161 cttctgccag agggcctttg aggagtttgg caaaaacttc caaagccggg cgatcattga
     2221 tttcagaaat tagatttcct tcggccttcg taatgaccat cggttccgtg atcggttggc
     2281 aaccctgagt gatatcgatg accgcttcaa aggaacccga aagttgtaag ccggcgacag
     2341 aattcgattg gcacttttca ccgcaaactt gataggtcgc ctgcgtcatt ccgctctcgg
     2401 aggatccggc gccaactaca ggaacaaagc cgatcttttg ctccatgctg cgcagcaacc
     2461 ggtgcggctg gccattgtaa gtgtcaggaa acaaggctag caaagagctc tgatccgtac
     2521 cgaccatctg cgcgattctg cccccgactt cttcgtcgtg ctcgcgcaac ggctgaaata
     2581 ggaaaggctg actctgcatt tgatccgacg cgaacacaag caccgccagg ccgtgacggc
     2641 cctcaatttc accggcgccc gtgaggattc cggcgccgct ggagcccacg attcggtcgc
     2701 tgcgagtcac tcgggtcagt gtggacaaga ggcttttgct gtgaggagca tgttccgccg
     2761 taaaaaaaac taccacagcg tcggcctgcg agattcccgc ttgagccatc gcttcgagag
     2821 cggcttgctc agccgcccgc tccgttgatg ggttagagga ttgcccgact ccggcacgaa
     2881 tcataatgct cgaactatag gagacttacc gaggtcgggt caagcgaaac cattcacaca
     2941 ttattttttg attgaagagt caggcgaaat ccgatatttc taagacatta cccaccctac
     3001 aatcaggagc aaagaaaaaa tcgaatgaaa atcgtccaga ttccgctgct tcgggacaat
     3061 tacggctatc tcgtcgtctg tcaaaaaacc aacactgcgg gtattgtcga cccctccgaa
     3121 gccgaaccgg tctggcggcg tgtcgaattg gagaaggtca cactcacagc aattctcaat
     3181 acccaccatc atcgtgacca taccggcggt aacgaggggt tgctggcaaa acagagtctc
     3241 gaggtctacg gtcacaagag cgatcaggga aggattccgg gactcagccg cggcgttgat
     3301 gagggagatg agatcgtcat tggagaagag cgcggaagtg tccttttcat tccaggtcac
     3361 accacgggac atgttgctta tctcttcggt aataatcttt tttgtggcga cacgctgttc
     3421 actgccggct gcggtcgtct cttcgagggc acagcggaac agatgcacgg gtctctcaag
     3481 aagctcatgg ggcttcccga cgatacaaaa atctactgcg gccacgagta cacggaaagc
     3541 aatcttcgtt tcgcgatgac cctggaaccc aaaaatcaca aacttgtttc ccggttcgaa
     3601 agagtacagg ggttaagagc tcgcggtgcc tccacggtgc cttcgacgat ggaagaggaa
     3661 aagcaaacga atccgttcct gcgctgggat agcaaggaaa tccaggcgag attgaaagcc
     3721 ggttcgtccg gtctccgcct tgatccggtc tcggtatttg caactgtgcg gaagcttaaa
     3781 gacgcgttct agtgggtggc tgagtccggt tggccgcctt atacctgcgt ccgttcacac
     3841 ttccttcact cccagttcct tttcgagatt ggtcaagaat ccggtggcat cctcagtcag
     3901 tccccgaggg aaacgaaatg aaccttaagg aaaggaccta tctctggtac atcgccctcc
     3961 tgcgcattta catcggctac tatatgctgc agcagggaat cagaaagttt cagcgggatt
     4021 ttccgcgcgg cgactgggtc ggtcggcaaa tcggcgacct cgctaccatc gacctgtacc
     4081 catggtacaa gaagtttctt gttgattacg tggtgccgca tcaagagctg ttcggttatc
     4141 ttgtcatgct tggcgaaatc cttgtgggtg ggtgtctgct cctcggcttg ctgacgcgct
     4201 tgaacgcttt cgtcgctctg ttcatgctg
//
LOCUS       DASSWZ010000280         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_180564, whole genome shotgun sequence.
ACCESSION   DASSWZ010000280 DASSWZ010000000
VERSION     DASSWZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180564"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFN58_06835"
     CDS             <1..593
                     /locus_tag="VFN58_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET8917741.1"
                     /translation="RQILHTNDRVGKPKVESAKQTLQALNPDVKVITYQDKLTSANIM
                     DIIKDYEIIVDGCDNFPTRYLVNDACILANKPNVHGSIFQFDGQATVFYPGKGPCYRC
                     LYPEPPPADMAPSCAEAGVLGVLPGLIGVIEALEAIKLILGKGDSLVGRLLCFNTLTM
                     EINTLNLRRDPNCPMCGDHPTIHQLIDYEEFCSLRH"
     gene            complement(613..1386)
                     /locus_tag="VFN58_06840"
     CDS             complement(613..1386)
                     /locus_tag="VFN58_06840"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET8917742.1"
                     /translation="MERLQKIISRAGLASRREAERWILEGRVSVNGAVISRLGSQADP
                     AKDSIKVDGKRIKPATAPLYFVFHKPPGVITTLNDPQKRPDLTRFIEKLGSKQRVFPV
                     GRLDYNSTGLLLLTNDGELARRLTHPRFGVKKVYQVKLSACPTAEEFALLRKGIRLED
                     GVAAPARARLLEKLKKNAWVEIEIHEGRNREVRRIFETLGYFVEKLIRVRVGPIALGF
                     LQPGEIRPLSQIEIKALKSGVGLLSPQDSPLTPHIKGKG"
     gene            complement(1541..2116)
                     /gene="scpB"
                     /locus_tag="VFN58_06845"
     CDS             complement(1541..2116)
                     /gene="scpB"
                     /locus_tag="VFN58_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET8917743.1"
                     /translation="MEREDVRSIIESLLFVADGPLTIQRLGEVLEGAEKEDVRSILDE
                     LQAELENSRRGVRLVEVAGGYQLRTAKVNADWVKKFLGGRPARMGRATLETLAIIAYR
                     QPITRAEIEAIRGVDVDGVINTLLDRSLIRAVARKDVPGRPFLYGTTPEFLQLFNLKD
                     LTHLPTLKEMEEITLPEIPGEELPLKSAEEC"
     gene            complement(2106..2879)
                     /locus_tag="VFN58_06850"
     CDS             complement(2106..2879)
                     /locus_tag="VFN58_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET8917744.1"
                     /translation="MTTRVQLEIFEGPLDLLLHLIKKNEVSITDIPIAMITEQYLATL
                     EVMQTLNLDVAGEFLVMAATLIHIKSRMLLPLTDGEEDEEDGGADPREELMRRLLEYQ
                     RFKDAADQLERREVLTRDVFVRSVAPAEEVPAPGFREVSVFELLTALKQVLDRLPKDV
                     VHEVMLDKITVREKMTLLLDHLRAQGKTLFESLFAEVKTRMEVVVTFLAMLELVKVRA
                     IRIFQEEATGPIQIEAAAGIENAPAVLAIQDPEEDQHGT"
     gene            complement(2876..>3643)
                     /gene="trpS"
                     /locus_tag="VFN58_06855"
     CDS             complement(2876..>3643)
                     /gene="trpS"
                     /locus_tag="VFN58_06855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET8917745.1"
                     /translation="LDPDRSTIFRQSQVKEHAELHLLFSMITPVPWLERNPTYKDQLK
                     ELVEKDLSTYGFLGYPVLQAADITIYKANKVPVGVDQAPHVELTREIVRRFNQIYRPI
                     FPEPEVLLTEAQKVPGLDGRKMSKSYNNAVFLSDTPQEIDQKLSRMMTDPARVRRTDP
                     GEPEKCPAFQLHKIYCTPEEIAEVSTGCRTAGIGCLDCKKVMIKHVIEDLAPFREQRA
                     RYEKDPEQLEAVLTTGTAIAHEKAAETMVEVREAVGL"
BASE COUNT          784 a         1055 c          943 g          861 t
ORIGIN      
        1 aaagacagat tcttcacacc aacgaccggg tcggcaaacc gaaggtagag tccgccaagc
       61 aaacacttca ggcgctaaac ccggacgtga aggtgatcac ttatcaggac aagcttacgt
      121 ccgcgaatat catggacatc atcaaagact acgaaatcat cgtcgacggc tgcgacaact
      181 tcccaacgcg ctacctggtc aatgatgcct gtattctggc gaataagccg aacgtgcacg
      241 gaagtatctt ccagttcgac ggccaagcaa cggttttcta ccctggcaag gggccttgct
      301 accgatgcct ctatcctgaa ccgcctccgg cagacatggc gccgagttgc gctgaagccg
      361 gggtgctggg agttctcccc gggctgatcg gggtcatcga agccttggaa gcgatcaagc
      421 taattctcgg caaaggcgac agcttggtcg gccggctcct ctgtttcaac acgctgacca
      481 tggagatcaa tacgctgaat ttgcgccgcg atccaaactg tccgatgtgc ggcgatcatc
      541 ctaccattca ccagctcatc gactacgaag agttctgcag cctgcgccac tagtttctgt
      601 ctccttaacg attcaccctt tacccttgat gtgaggggtg aggggtgaat cctgagggga
      661 gaggagtccc acgccgcttt tcagggcctt gatctcgatt tgcgacagcg gtcggatctc
      721 accgggttgc aaaaaaccga gggctatcgg gccgacgcgc acgcggatca gcttctcgac
      781 gaaatagccc agggtttcga agatgcggcg aacttcgcga ttgcggccct cgtgaatttc
      841 aatctccacc caggcatttt tttttagttt ctccagtagt cgagcccgcg ccggggcggc
      901 taccccgtct tccaagcgaa tccctttgcg caacagcgca aactcctccg ccgtcggaca
      961 agcgctcagt tttacttggt agactttctt cacgccgaaa cgggggtggg taagccgccg
     1021 ggccagttcg ccgtcattgg tcaaaagcaa aaggccagta gaattgtaat ctagtctgcc
     1081 cactggaaaa actctctgtt tgctgcctaa cttttcgatg aaacgcgtca gatccggacg
     1141 cttctgcggg tcatttagag tggtgatgac tcccggcggt ttatggaaca cgaaatagag
     1201 cggcgccgtc gcgggtttaa ttcgcttgcc gtcaactttg atactatctt tcgcgggatc
     1261 ggcttgggag cccaatctgg aaatgaccgc gccgttgacg gatacgcgtc cctccaggat
     1321 ccatcgttcc gcctcgcgac gcgaagccag gcccgcgcgg gagattattt tttgtaagcg
     1381 ttccattctg ggagttcaaa gttccaggtt caaagttcaa aggacagaca gtctcgtcga
     1441 cgttgaactt tgaaccttga actctttaag gagccacagg cgcattgccc gcgaccggag
     1501 gggcttactc ctcggttctt ttatccgtga atagcgcggg tcaacactct tcggcactct
     1561 taagcggcag ttcctcgccg ggtatttccg gcagggtaat ctcttccatc tctttgagcg
     1621 tgggcaaatg agtgagatct ttgagattga ataactgcag aaactcgggc gtcgtcccat
     1681 agagaaaggg ccttccgggg acatccttgc gcgctacggc gcgaatcaag ctacgatcca
     1741 aaagggtatt aatgacaccg tccacatcca caccacggat ggcctcgatt tccgctcgag
     1801 tgatgggttg tcgataggca atgatcgcca aggtctcgag agtggctcgt cccatgcgcg
     1861 ccggccggcc gcccaggaat ttttttaccc aatcagcatt gactttcgcc gtccgcaact
     1921 ggtaaccgcc ggcgacctcg acgagtcgaa ccccacgccg gctgttttcc agctccgcct
     1981 gcagttcatc gagaatcgac cgcacgtctt ccttctcggc gccttccagc acttcgccca
     2041 acctttgaat ggtcaagggg ccatcggcga cgaacaacaa actctcaata atcgacctta
     2101 cgtcttcacg ttccatgctg atcttcctca gggtcctgga ttgcaagaac cgcgggagca
     2161 ttttctatac ccgccgccgc ttcgatctga attggcccgg ttgcttcttc ttgaaagatt
     2221 ctgatggcac gcactttaac cagctcaagc atcgcgagaa acgtgacaac gacttccatc
     2281 cgggtcttca cctcagcaaa aagcgattca aacaaggttt tcccctgggc gcgcaaatga
     2341 tcgagcagca gggtcatctt ctcgcgcacg gtgatcttat ccaacatcac ctcatggact
     2401 acgtctttag gcaagcgatc aagaacctgt ttgagtgccg ttaggagttc gaataccgag
     2461 acttcacgaa atcccggcgc cgggacttct tcggccggcg ccacggaacg aacgaacaca
     2521 tcccgcgtca atacttccct tcgttccagt tgatccgcgg catccttgaa gcgctggtat
     2581 tcgagcagcc gccgcattaa ctcctcgcgt ggatctgccc ccccgtcttc ttcgtcctct
     2641 tcgccgtcgg tgagcggcag cagcatacgc gacttgatgt gaatcagcgt agccgccata
     2701 accaaaaatt cgccggcgac atcaagattt aaagtctgca tcacctccaa ggttgccagg
     2761 tattgttcgg tgatcatcgc aatcggtata tcggtgatgc tgacctcatt ctttttaatc
     2821 agatgaagca gcagatccaa cggcccttcg aaaatttcga gttgtactcg ggtggtcaca
     2881 gtcctacggc ctcccgcact tcaaccatgg tttcggcagc tttttcatga gcgatcgctg
     2941 tgcccgttgt cagcaccgct tcgagctgct cagggtcttt ctcatagcgc gccctttgct
     3001 cgcggaaggg cgctagatct tcgatcacat gcttgatcat caccttcttg caatccaggc
     3061 aaccgatgcc ggcggtgcgg cagcccgtgg aaacttctgc gatctcttca ggggtgcaat
     3121 aaatcttgtg cagttgaaac gccgggcatt tttccggttc accgggatcg gtccgtctga
     3181 cccgcgcggg gtcggtcatc atgcgactca atttctggtc gatttcctgc ggtgtgtcgg
     3241 acaaaaacac ggcgttgttg tagctcttgc tcatctttcg cccgtccaac cccggcacct
     3301 tctgtgcctc ggtcaggaga acttcaggct ccgggaaaat gggacggtag atctggttga
     3361 agcgccggac aatctcgcgg gtcaattcga cgtgcggcgc ttgatcaact cccacgggga
     3421 ctttgttggc tttatagata gtaatgtccg cggcttgcaa caccggatag cccagaaagc
     3481 cgtaggtcga cagatccttt tccaccaatt ccttgagctg atctttatac gtgggattgc
     3541 gctccaacca tggcacaggt gttatcattg aaaaaagcaa gtggagctcc gcatgctctt
     3601 tcacctgtga ttgccgaaaa atagtcgagc gatccggatc gag
//
LOCUS       DASSWZ010000281         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_577121, whole genome shotgun sequence.
ACCESSION   DASSWZ010000281 DASSWZ010000000
VERSION     DASSWZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577121"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1791)
                     /locus_tag="VFN58_06860"
     CDS             complement(64..1791)
                     /locus_tag="VFN58_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8917746.1"
                     /translation="MSYDLSASSSPRLRLRVRPFRIAEAFFVLLFGVAMGLPLLFLLT
                     GSFNLAPPGREAVYGIANWARAFSDPGTVSALWMSFLIAVVRLIPAMILSVVVAWLIA
                     RTDMPGRNAFEFLCWFAYFVPDFPLVLAWILILDPNFGLLNTAVAILPFAEGPLFNPY
                     SFWGIIWVHTSVSGIWFKVMLLTPVFRRLGASLEEAARVAGANGFTTLWRVTLPVLAP
                     MIIAVAALSFIRGLESFNTELLLGLPAGIYVYATRIYDYTRQEPPAFGEATALGSVFL
                     LVLIVLAFFYHRYLMGNRKFTVVTGQGYSTAPVKLGKWRYVASGGCLLYFSVMMLLPL
                     AFLVIGSFMRRYGFFGLKSPFTLAHWQNLLADPVFFVSLKNSLIIATLTALAGIVLYS
                     LSAYLIVSKRSRAAQLMDLLCWVPWVVPGILMSLGLLWIFLATPLRTFLYGSVWGIAL
                     AMTIKDSPVSTQAFKAAFLQLGQELEEAARVTGASWWYTYRRILLPLLAPMVATVGLL
                     NFGSALTSISTPVLLYSAHSRPLSILLMEYSFTGELERAAALGLLITVIISLMMLLGR
                     KLGLQLTQS"
     gene            complement(1788..>2189)
                     /locus_tag="VFN58_06865"
     CDS             complement(1788..>2189)
                     /locus_tag="VFN58_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET8917747.1"
                     /translation="EKAGTPFVAEFLGRTVLLEGTIVSNHFGCWFELPNGAGRLLLEG
                     QQWTDQLSARLFVRPEDIEILSSGEPSQHEVRATIEEVAYLGDRFEYSVRAGGKQFVV
                     SAAKKNRYPAGSEVRLRFDPVRLHPRLVDDP"
BASE COUNT          578 a          622 c          618 g          371 t
ORIGIN      
        1 tgcacgaagc attttacgcc tccgcttttc cgggtcgttt cagcgaaacg aacacgaacc
       61 tctttaggat tgagtcaact gcaatccaag ctttcttccc aagagcatca tcaatgagat
      121 aatgaccgtg atcaaaagcc ctaatgctgc cgcacgttct aattctccgg tgaaactgta
      181 ctccatgagc agaatcgaaa gcggcctcga atgagcgcta tagaggagca ccggagtact
      241 gatgctggtg agcgcgctac cgaaatttag cagccccacg gtagccacca ttggcgccaa
      301 aagcggcagg agtatgcgcc ggtacgtata ccaccaggaa gccccggtca cgcgcgccgc
      361 ctcttccagc tcctgcccaa gttgcaaaaa ggccgccttg aatgcttgag tcgaaaccgg
      421 actatctttg atcgtcattg ccagggctat accccaaacc gagccgtaga ggaaggtgcg
      481 cagcggcgtt gccagaaaga tccagaggag gccgaggctc attagaatcc ccgggaccac
      541 ccaagggacc cagcagagga gatccatgag ctgggccgcg cggcttcgct tggaaacaat
      601 gaggtaggcg gagagcgaat aaagaacgat acctgcaagc gccgtcagag tcgcaataat
      661 gaggctgttc ttcaacgaca caaaaaagac cgggtcggcc agcaggttct gccagtgcgc
      721 caatgtaaac ggcgacttga ggccgaaaaa accataacgg cgcatgaaag agccgatcac
      781 cagaaaggcg agcggcaaga gcatcatcac tgaaaaatag aggagacatc cacccgaagc
      841 cacgtagcgc catttgccca gttttaccgg cgcagtcgag taaccctgtc cggtgactac
      901 ggtgaatttt ctgttcccca taagataccg atgataaaag aaagccaaga cgatcaagac
      961 taggagaaag accgagccca aggcggtcgc ctcaccgaag gccggaggct cctgccgtgt
     1021 gtaatcatag atgcgcgtcg catagacata gattcccgcc ggcaagccga gcagaagctc
     1081 ggtgttaaag gactcgagcc cgcggataaa gctcagcgcc gcaaccgcga taatcatcgg
     1141 cgcgagcacc ggcagagtga cacgccacaa ggttgtaaaa ccgttggcgc cagcgactcg
     1201 cgccgcttcc tccaagctgg ctccgagccg gcgaaacacc ggggtcaaga gcatcacctt
     1261 gaaccagatg ccgctgacgg aagtgtgaac ccatatgatt ccccagaaac tgtatgggtt
     1321 gaataggggc ccctccgcga acggcaaaat cgcgactgcc gtgttgagca ggccgaagtt
     1381 gggatcgaga atgagaatcc acgcgagcac caacgggaaa tcaggcacga aataggcgaa
     1441 ccagcaaaga aactcgaagg catttctgcc gggcatatcg gttcgagcga tcaaccaggc
     1501 aacgaccact gaaaggatca tcgccggaat gagccgaacg acggcgatca ggaaactcat
     1561 ccagagcgcg ctgacagttc cggggtcgga aaaggcgcgc gcccagttcg ctattccgta
     1621 aaccgcctct ctccccggcg gcgccaggtt gaagctgccg gtcaagagaa aaagtaacgg
     1681 cagccccatg gctacgccga acaatagaac gaagaatgct tccgcaatgc ggaagggccg
     1741 gactcgcaat cgcaggcgcg ggctagacga tgctgataga tcgtagctca tgggtcgtcg
     1801 acgagtcgtg gatgcaaccg gaccggatcg aaacggagac ggacctccga gcctgccggg
     1861 tagcgattct tttttgccgc tgacaccacg aactgtttcc cgcccgccct cacgctatat
     1921 tcgaagcgat caccgaggta ggcgacttct tcgatggttg cgcgcacttc atgctgggaa
     1981 ggctctccgg atgaaaggat ctcaatatct tccggtcgca caaataagcg agcagacaat
     2041 tggtcggtcc actgctgccc ttcgagcagc aaccgtcctg cgccgttggg aagctcgaac
     2101 cagcagccga agtgattgct gacaatagtt ccttcaagaa ggacggtgcg gccgagaaac
     2161 tcggcgacga aaggcgtgcc ggccttttc
//
LOCUS       DASSWZ010000282         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_887793, whole genome shotgun sequence.
ACCESSION   DASSWZ010000282 DASSWZ010000000
VERSION     DASSWZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887793"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1162..1533
                     /locus_tag="VFN58_06870"
     CDS             1162..1533
                     /locus_tag="VFN58_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917748.1"
                     /translation="MGIDSEKTVLIVDNQPREAMALQKQLAFAGFKTEIASTGEGAVK
                     ILEEHPPDIVLLEASLSDIDSGEVVTFIRSDLRTCRLPVLAMSVFPHMKGRCLEGGCD
                     DFLQKPIKILDLVTRIRKALR"
     gene            2146..2964
                     /locus_tag="VFN58_06875"
     CDS             2146..2964
                     /locus_tag="VFN58_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HET8917749.1"
                     /translation="MSIVTISHAPYSGGSEIAEKLALVLNFRCVDREVLLEASRRYGI
                     PEAKYTEVLEAEGHWWERWMESLRLYRITLQAAMCEVAQGGRLIYYGRAGQELFPGVR
                     HVLKVLIVAPMEYRMQEVKARKGLEGENARHFLRELDRVRSRRLRALFNTDWQDPVGY
                     DLVINSSRINTDSAAHLIGETVSREEFRPTPESEKAFQDLMTTARVQAALITSPKTRN
                     IILNVRSDGGQVNISGILADPDLETEIARIAKGVAGVTHVTTDIEPPPIEYMHP"
     gene            3098..3625
                     /gene="tadA"
                     /locus_tag="VFN58_06880"
     CDS             3098..3625
                     /gene="tadA"
                     /locus_tag="VFN58_06880"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET8917750.1"
                     /translation="MESLPDNDLKDARYMLVALEEAKKAAASGEVPVGAVVVCRSEII
                     GRGYNRREELRSALAHAELLAIEQASRHLGSWRLCDCQLYVTLEPCVMCVGAIVQARM
                     GRLIFGCLDPKGGAVESLYQLCDDSRLNHRLPVAGGMLAADCSAILEGFFNRLRQNRR
                     DQAKAERWPSPAEGA"
     gene            3592..3683
                     /locus_tag="VFN58_06885"
     tRNA            3592..3683
                     /locus_tag="VFN58_06885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3628..3630,aa:Ser,seq:cga)
BASE COUNT          889 a          969 c         1092 g         1009 t
ORIGIN      
        1 aaagacggtt caaggaacat cagatcacta tctgtcacga gacagtgaga aagggaagcg
       61 ccttgcatcg atgcctaagt tggcgttagg ggggccgtct acagttcgaa ctccttcgtt
      121 ccctttgcaa ccaactagat attccagaga aggaatttgg cgtcgatatt ccctggtagt
      181 ctcgggcttc ggtgtagctc gggcaccatt tcctccccgg caaattgttg gctaaacccg
      241 tctgtatttt catgatggtt attaatttcg atgtgtggtt gctctccatt tcttggggtc
      301 gagtcgggga ccgtcaagga ggctcatatg accgagatgc ttcaagtgcc gctttccttc
      361 tgagcctctt ttggatcccg gactagccta actttttacc ggtgtcagtg ccgccaccta
      421 tttgctagct gggggaggcg ccgatcttcc gctgaccttt tgggggacat tgatttccgt
      481 accaacctgt atcgacatat aatgaatttt tcgtgaacaa tgtctctatt agctgggatt
      541 cacgatgagg actaacaccg gactgtagga taaggggttc agaccccttc gggcgcgccg
      601 gcggctggca gccaatgtgg tgcaactcct cttggaccgt tgagttggag gatgactgtg
      661 aagatgaagt tcccggattt tcgcctgcca agcgctatat cgtcgaaacg gcatctctgg
      721 ttcttgattt ccatcaatat tttagtgatg tcgtgtgcaa ccaccagcac gacaacgcag
      781 tctcggcgcc aagcgcagga gccccgcggg tttttcggaa gtcttatgga tcagatgaca
      841 gagcgggagt gcaacgtcgg ccgatttatt tgtcccttcg gtttaggtcc agcaggagag
      901 gcttgtacgt gtacggatcc ctccggagtt gttttaaccg gccgcacgat caagtagtct
      961 tgaagtcgga gcgatccgtt gcctcaagct aagcgatcag ctttggctga ataccacccc
     1021 cgaaaaattc tcggttcttg gaatcgcctt ctggcgcgat ttggacgtta gtgtcgtccg
     1081 gtttccgaga ttcatagccg agcggtttga aggtccttcg ttgggttgcc gatatttcga
     1141 atccggataa taatattggc cgtgggaatt gatagcgaaa agaccgttct catcgtcgat
     1201 aaccaaccga gagaggcaat ggcgctgcaa aagcaactcg cattcgccgg ttttaagacc
     1261 gagattgcca gtaccggcga gggtgccgtc aagatattag aagagcatcc acccgacatt
     1321 gtgctgctcg aagccagttt gtcggatatc gatagcggtg aggttgtgac ctttattaga
     1381 agcgacctgc ggacgtgccg cctgccggtt ttggcgatga gcgtgttccc ccacatgaaa
     1441 ggtagatgcc tcgaaggggg ctgcgacgac ttccttcaaa aaccgataaa aatcctcgat
     1501 ctcgtgactc gaatcagaaa ggctcttcgg tagccggcgt tatccacgta agcggaaccg
     1561 agggagcagg cctgtgctca ctcgtctatc caaaagattt gtcgtaccta ttcctcggtc
     1621 catgttccgg gctaacaggt cagccccatc ttaaaatctt gaggcattca gttgccttct
     1681 gagccaatga ttcagtacgg agcgcttgaa gcgccactgt gatcctatct taaaagcggg
     1741 tcgcctcttt tgcgcaacca gccgtaacct gtgtattggt taacgcgcag atattccgca
     1801 acctctttcg ttgtcaagaa gggtcgtgtg gtcatagcag caagtcctta ggtgcaattg
     1861 gtcaacccta tgatgacgat cctttcggta gattaagtta ttgtttcacc gtagttcact
     1921 ttttgttgac atcggtttcc taaggcggac gggtgagcac cttgtcaaga cggttgggtc
     1981 gcaattattt caaaccttga cgattttgag aagaatgggc ttgacgagtt aaagctcata
     2041 gtgggagaat gcgccccgaa atcgcggagc agcaagagct gagcaatttg taaaacctgg
     2101 ccaggagtat aggtcggtca aacaattcgg caaaggaggc tacttatgtc gattgtaact
     2161 atttctcatg cgccctacag cggcggcagc gagattgctg aaaaattagc cttagttttg
     2221 aatttcagat gtgttgatcg tgaagtattg ctagaagcga gccgccgcta tggcattcct
     2281 gaagccaaat acacggaagt tctcgaggct gaaggccatt ggtgggagcg atggatggag
     2341 agcctgcggc tctatcgtat cacgcttcag gcggccatgt gcgaggtggc acaagggggt
     2401 cgactgatat actacggccg agcaggtcag gagctttttc caggagtacg gcacgtcctg
     2461 aaggttttga ttgttgctcc catggagtat cggatgcagg aagtgaaggc gcgcaaaggc
     2521 cttgaggggg aaaatgcaag acactttctt cgtgaactcg accgagttcg gagccgtagg
     2581 ttaagagcgt tattcaatac ggattggcaa gatccggtgg gctatgacct cgtgataaac
     2641 agctctagaa taaacacgga tagcgctgca catttaatcg gcgagacagt aagtcgagaa
     2701 gaatttcggc ctacaccgga atccgaaaag gcctttcagg atcttatgac gacggcgcgc
     2761 gtacaagccg ccctcataac gtcgccaaaa acgcgcaaca ttattctcaa cgtccgcagc
     2821 gatggagggc aagtcaatat ctcaggtatt cttgccgacc cggaccttga gacagagatc
     2881 gcgcgcattg cgaaaggtgt ggcaggtgtc acccatgtta cgaccgacat tgagccgccg
     2941 cccattgaat atatgcatcc ttagttgtgc caacctattt tcttgcggcc tctgtgtttc
     3001 ctttccgatc caccgatttg gttgccggag ccgaccgtga ataatgtgcc gctgaatttt
     3061 cttggctgtt gtcttgtgct atcgtcgagt ggtcttgatg gaatctttgc ccgataacga
     3121 tcttaaagac gcccggtaca tgcttgtagc gttggaggag gcaaaaaaag ccgctgccag
     3181 tggtgaagtg ccggtgggcg ctgttgtcgt ttgccgaagc gaaatcatcg ggcgaggtta
     3241 caatcggcgg gaagagctgc gcagcgccct cgcgcatgca gagctattgg ctatcgaaca
     3301 ggcgtcccgt catctgggca gttggcggtt gtgcgactgc caactttacg tgacgctcga
     3361 accgtgcgtc atgtgtgtgg gagccatcgt tcaggcacga atgggtcggc tcatttttgg
     3421 ctgtcttgat cccaagggcg gcgcggtaga atctttgtat cagctctgcg atgattctcg
     3481 tctgaatcac cggctgccag tcgcgggtgg gatgctcgct gcagattgct cggcgattct
     3541 tgaagggttc tttaaccgtt tgagacagaa cagacgggac caagcaaaag cggagaggtg
     3601 gccgagcccg gctgaaggcg cctgactcga aatcaggttc acccggaagg gtgacggggg
     3661 ttcgaatccc tccctctccg ccaaacccta attccttgat tcagcgtgag tttctccccg
     3721 tttcctggtt cccagtcaga agcctgtgaa tgaaaaatca agttacccct accctcgtaa
     3781 agtgcccttt tgtgcatgaa ctgtgaagga acttttggat acacgttaag ccgtttctcg
     3841 cttcagtggt acaaccttcc gaatctcagc ctctttcttc aagtgttctt ggacccgatt
     3901 catggcctcg gtcaagtccc gatcatcgaa gatattatac cgatcgtaga cgctccgcg
//
LOCUS       DASSWZ010000283         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_435567, whole genome shotgun sequence.
ACCESSION   DASSWZ010000283 DASSWZ010000000
VERSION     DASSWZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435567"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            888..1922
                     /locus_tag="VFN58_06890"
     CDS             888..1922
                     /locus_tag="VFN58_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET8917751.1"
                     /translation="MLSITAGLYNLFVIVWFCCYLQWRDSVWWLALLNALAPLFFAPI
                     LIYFALGIIAPSIAAWIGVVVPALIFLYFYGSLFWFNPSLPAETAGDPVTIMTFNIWW
                     HSRSDQTAQVITQNGSIDVLALQELREHMAQHLIDAWSAHYPYRLVHTGTGTPRLGIF
                     SRYPLIPVATDHLAAFNFRIQAARVIAPAGEFLLYNVHPRAMNFREYIYEHKSIAREA
                     AHSIQMQLRHIEDLLRDIDSQDQPVVVVGDFNCTPQSTVYARLLSQLVDGHRSAGWGF
                     GHTFPAHGKGLRGLPVPFPFMRLDMLFHSKELVTLHSRVGKRHGDSDHFPVITTLTWQ
                     APQPETRLCS"
     gene            1913..>2172
                     /locus_tag="VFN58_06895"
     CDS             1913..>2172
                     /locus_tag="VFN58_06895"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HET8917752.1"
                     /translation="MFVDQSFVIRGALAAYSVSYHEVGRLSAKYVQRILAGVKPKDLP
                     VQQVDKIELVINLKTAKQIGLTIPPNVLVRADKVIKVMAENQA"
BASE COUNT          489 a          616 c          563 g          504 t
ORIGIN      
        1 aatacaaccc gtcaaaggaa aggcagtcaa aacaatcgca aggactccat agcgaaaggc
       61 attaactaaa gttgtcaaca cgttctcctc ctgacttgac atcgaccact cgccccccgg
      121 tccctgtttt atccctcatc actcgactcc tcgccccgtc tgttctaatc gacccaatgt
      181 agcacgttac ggataaccga ccagcgcggc tcgcgtttag cgacctgctt cccgagaatc
      241 cagtgccctc taggaatgcc atgtgctatc cgcctttctt ccaacagaac taatgaataa
      301 agttcgctta ttatattacg ggcgacaaaa attatcgaat ccatcagaga agcatctgcc
      361 gctatggcgc gcgaacgaga aattgagggg gagcggagcg ggtctgtctt gacagcgcat
      421 cactggtggg cactagcaac tgcgcagccg cccaggctaa aaatattgcc ttcccactcg
      481 acgactacct gtcgcctttc gccgccgtaa atcacacttt cctttatctt tatccgcgtg
      541 atagataacg ctcgaaacga ggtgcgcaat gaagaaggct gcgatgccat caatccttgt
      601 tgcagtggta ctgcttgcgg ttgcggtcat agccgttacg cggagcgtgt ggtggccgag
      661 gaatgcaaga agagcggagt gtcgctaacg gaactccgaa gcgggagtcg tcgtggccgg
      721 ttgcctgcgg tgagaacgag gatagttcgc ggactcgtgg agaattacgg cgtaggggtc
      781 gccgaggttg ctcgccgtgt gggaatctcg acttccgggg tttcgaagat cctaacgaga
      841 acgttgtcca gctaatcaac agcgtcccct cgacggtcct aacgctcatg ttatctatca
      901 ccgccggatt gtataacctc ttcgtcatcg tctggttttg ctgctatctg caatggcggg
      961 attcagtctg gtggttggct ctccttaatg cgcttgcccc tctgtttttt gcacctatct
     1021 tgatttattt tgcacttggc atcatcgcgc catccatcgc ggcgtggata ggcgtcgtcg
     1081 taccggcgct aatctttctc tatttctacg gttctctttt ctggttcaac ccatccctgc
     1141 ccgccgagac ggctggcgat ccggtcacga ttatgacgtt taacatctgg tggcatagcc
     1201 ggtcagacca aacagcgcag gtcataacgc agaacgggag cattgatgtc ctagctctcc
     1261 aggagttgag agaacacatg gcgcagcatc tgatcgatgc ttggagcgct cactacccct
     1321 accgtctggt gcacacgggg acgggcacac ctcggctggg catcttcagc cgctacccac
     1381 tcattccagt tgcaaccgat catttggcgg cgttcaattt tcgcattcag gcggcacgcg
     1441 tgatcgcacc ggcgggggaa tttttgcttt ataatgtcca cccacgcgct atgaatttca
     1501 gggaatatat ctacgagcac aaatccatcg cccgcgaagc ggcgcactcg atccagatgc
     1561 agttgcgaca catcgaggac ctgctcagag atattgacag ccaagatcag ccagtcgtgg
     1621 tggtgggcga cttcaactgc acgccacaaa gcaccgttta tgcccgattg ttatcccagt
     1681 tagtcgacgg gcaccgctcc gctggatggg ggtttggaca cacatttccc gcccacggca
     1741 aaggtttacg cggtctgccg gtaccgttcc cattcatgcg tttggacatg ctcttccatt
     1801 cgaaagaact cgtgacactc cacagccgcg tcggaaaacg acacggtgat tcagaccatt
     1861 ttcccgtcat cacgaccttg acatggcaag caccgcagcc agagacccga ctatgttcgt
     1921 agatcaaagt tttgtcatca ggggagccct cgccgcctac agcgtgagct accacgaagt
     1981 tggccgcctg tcagcgaagt acgtccagcg gattcttgct ggtgttaaac ccaaggactt
     2041 gccggtgcag caggtcgata agatcgagct ggtcatcaat ctcaagacgg ccaaacagat
     2101 cgggctgacg attccaccca acgtgctggt aagggcggat aaggtgatca aagtgatggc
     2161 agaaaaccaa gc
//
LOCUS       DASSWZ010000284         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1397750, whole genome shotgun sequence.
ACCESSION   DASSWZ010000284 DASSWZ010000000
VERSION     DASSWZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397750"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VFN58_06900"
     CDS             <1..805
                     /locus_tag="VFN58_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917753.1"
                     /translation="GASLFLGLTILATMLLDQKIAEIIGVGNQALLFLAFVGTLWGFI
                     FHQKKTPSPMIDLSFFSIPVFGYGSIGLLMACITQGLTTFVTPFYLQDVLELSPTFMG
                     IIFLVPSLLSMVLSPVSGALTDRIGARFLLITGVLFLMAAFLIGAYLRVDSHWILPTS
                     LLALVGIGSALFNTPSQAVMVSSLPKEHWGIAIGIINGIFGLGHMLGISLSGIFLTLA
                     FRFYSGDPGTTPNPGDTQVFVASMNVTYLFALGISLIPLLTSIKSRKII"
     gene            complement(945..2243)
                     /locus_tag="VFN58_06905"
     CDS             complement(945..2243)
                     /locus_tag="VFN58_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917754.1"
                     /translation="MNLKHLANFFSRIFYGWRMIGLVSAIRVVGGGLHQFGFTVFFLP
                     ISQDLGIGRAAMSLAFSLSRAQGAIEAPLVGYLIDRYGPRPTLVTAVFLAGVGYILLS
                     WVNSYTGFMIVYLGVICLAFVAGFVHSPMVVANSWFIRQRARAMTVVSAAVPVGGALI
                     APLLAIGVTSIGWRWAAFVSGCIFLLVCLPLSFQLKRSPESMGLLPDGDVASVAGTIS
                     SADTTNKNIRAELTSREAMKTWVFWVLVVSMTARVTCYSAATVHFVPLMVWKGLSEGA
                     AASLLGAFAFANLVVHFVLGWIADRVNKPKLLAACNLLPALSVAPLLWDSGNWQFWFF
                     TILFTLLDASFPIVWATVGDFFGRRHFATVRGSMSFFYMWGSFAGPVLAGAIYDRTQS
                     YMTVLWIWFSLLTFATLLLLFLIRPWTNRMMEIKPMPQPQ"
     gene            complement(2401..2814)
                     /locus_tag="VFN58_06910"
     CDS             complement(2401..2814)
                     /locus_tag="VFN58_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HET8917755.1"
                     /translation="MRVVLDTNVVVLALLFTGISSKLVQLWQGGAITALVSRSIVEEY
                     LRVLSYPKFKLSEGDIRGLIQEELLPYVEVVKIGRRLRVVDRDPSDDKFVECAVAGKA
                     RVIISGDKDLLSIGRYRKTLIQSPAQFLEQNPQLR"
     gene            complement(2814..3047)
                     /locus_tag="VFN58_06915"
     CDS             complement(2814..3047)
                     /locus_tag="VFN58_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET8917756.1"
                     /translation="MLAKKTVKNQITLPKAIIEKLPKTDYFEVSIRGEEIVLRPVVIE
                     AKGTRLEKVRDKMKALGLTEKDVDAAIRWARTR"
BASE COUNT          779 a          874 c          833 g          710 t
ORIGIN      
        1 gggagcgtcg ctcttccttg gattgacgat tctcgccacc atgctgctgg atcaaaaaat
       61 cgcggagatc attggtgtgg gcaaccaagc gctgcttttt ttggcttttg tgggaacact
      121 ttggggcttt attttccacc agaagaagac cccgagcccg atgatagatc tctcgttttt
      181 ctcgatcccg gtttttggtt acggctccat tggcctgctc atggcctgca tcactcaggg
      241 gctcactacg tttgtcaccc ctttttatct ccaggacgta ctagagcttt cgccgacgtt
      301 catggggata atctttctcg tcccgtcgct cctcagcatg gtgctatcgc ctgtgagtgg
      361 cgccctgacc gatcgtatcg gcgcccgctt cctcttgatc accggtgtgc tatttctgat
      421 ggctgctttc ttaattggtg cttaccttcg ggtcgactcc cattggatct tgcccaccag
      481 cctgctcgcg cttgtgggta ttggatcggc cctttttaat acgccatcac aggcggttat
      541 ggtaagctca cttcctaaag aacattgggg catagccatc ggtattatca atggcatttt
      601 tggcctggga cacatgctcg gcatctcgct gagcgggatc tttttgacct tggcgtttcg
      661 tttttattca ggggatccgg gaaccacccc caacccggga gacactcaag ttttcgtcgc
      721 ttccatgaac gtcacctatc tgttcgccct tggaattagt ctcatcccat tgctgacttc
      781 aataaagagc aggaagatca tttgaaaggc actccaaatg cgggtcaacg acctttccga
      841 tcccgacatt ctcgcttcgg gaaatcgtcg aagccgccca cgaacaatcc cagatatcgc
      901 gaacgcttta gcagagcact gtgcagattg agcatgattt ttactcattg cggttggggc
      961 attggcttga tttccatcat ccggtttgtc caaggccgga tcaaaaacaa aagcaacagc
     1021 gtcgcgaaag tcagcaggct gaaccaaatc cacaggacgg tcatataact ctgagtccgg
     1081 tcgtaaatcg cgccagcgag cacgggaccg gcaaagcttc cccacatata gaaaaaactc
     1141 atcgatcctc tgaccgtggc gaaatggcgc cggccgaaaa aatctcccac ggttgcccag
     1201 acgatgggga atgaggcatc caacagagta aacaggatgg tgaaaaacca aaattgccaa
     1261 ttgcccgagt cccacaagag cggcgcgacg gagagagcgg gtagcaggtt gcatgcggcc
     1321 aagagtttgg gcttgttcac tctgtcggcg atccacccga gcacaaaatg gaccactaaa
     1381 tttgcaaagg cgaaggcacc gagaagggat gcggcggccc cttctgacaa tcccttccac
     1441 accatgaggg gaacaaagtg caccgtggcg gcgctgtaac atgtcacacg agcggtcatc
     1501 gaaacgacga gcacccagaa aacccatgtc ttcatggctt cgcggctcgt gagttcggca
     1561 cggatgtttt tgttcgttgt gtctgccgac gaaatcgtgc ccgccaccga agcgacatca
     1621 ccatcgggga gcaagcccat gctttccggc gagcgcttca actgaaagct caatggcagg
     1681 cacaccagta gaaagatgca gcccgagaca aaagctgccc aacgccagcc gatggaagtt
     1741 accccgatcg ccaacagtgg agcgatcaac gcgccgccca cgggcacggc ggcgctcacg
     1801 acggtcatgg cgcgggcacg ctggcgaatg aaccaactgt tggcgacgac catcggcgag
     1861 tggacgaatc cggccacgaa cgcgagacag atcacgccca gatagacgat catgaagccc
     1921 gtatagctat tcacccaaga gagcaggatg taaccgacgc cggcaagaaa aaccgccgtt
     1981 acgagggtcg gccgtgggcc gtagcgatcg atgagatagc cgacgagcgg cgcttctatg
     2041 gcgccctgcg cgcgcgagag cgaaaacgcc agtgacattg ccgcccggcc aattcctaga
     2101 tcctggctga tgggaagaaa gaaaacggtg aagccaaact ggtgcagccc gccgccgacg
     2161 acacgaatcg ccgagaccaa cccgatcatg cgccaaccat aaaagatccg cgagaaaaaa
     2221 ttggcaagat gcttgaggtt catgacgacg gcgagcaagc gttggcgccg acgttacgtg
     2281 aaagatgagt taagcgtcaa gggattggaa agatcgtgag gaaaaacggg tccgaacgga
     2341 caccaaggaa aagttttcgc aattgctcta aatgctaacg gttcatggtg tacggaagga
     2401 ttatcgaagt tgcgggttct gttccagaaa ttgtgctgga gattggatga gagtctttcg
     2461 gtagcggcca atggacagta gatctttgtc gccagaaatg attactcgtg cctttcccgc
     2521 gactgcgcat tcaacaaact tgtcatctga tgggtcacga tcaacgacac gcaaacgtct
     2581 ccctattttt accacttcca catagggtag gagttcttcc tggatcaagc ccctgatatc
     2641 tccttcggag agcttgaatt taggatagct aagcacacgc aagtattctt ctacgatact
     2701 tcgggaaacc aatgcagtga tagcgccgcc ttgccaaagt tgcacaagct tggatgaaat
     2761 gccggtgaag agtagagcca aaactaccac attggtgtct aaaacaacgc gcactaccgg
     2821 gtcctagccc agcggatcgc ggcgtcaacg tctttctccg tcaagccaag tgccttcatt
     2881 ttatcgcgga ctttttcaag ccgtgtcccc ttggcttcta tgaccacggg tctaagtaca
     2941 atctcctcgc cccgaatgct aacctcaaaa taatcggtct tgggaagctt ctcgataata
     3001 gccttcggca gggtaatctg gttcttgact gtcttttttg caagcataac tggattccgt
     3061 aaagtaaggc taccttacat cctgccagtg tcaagccgac aggggcagcc cgcggaatct
     3121 agagcctatt cacagaatgg acccggcgat actcgccgac ctttgacccg tcttattgaa
     3181 tccccaaagg aggtat
//
LOCUS       DASSWZ010000285         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_95595, whole genome shotgun sequence.
ACCESSION   DASSWZ010000285 DASSWZ010000000
VERSION     DASSWZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95595"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..481)
                     /locus_tag="VFN58_06920"
     CDS             complement(188..481)
                     /locus_tag="VFN58_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HET8917757.1"
                     /translation="MIYEILTQHVEPEKRDEYIKVFGDILKKANYAGSHGINFFTSIE
                     DPSRVIIMIAWDNVEAHTRHRCTPTHNAMREATARYQTQKSDGGHFVLHEVKG"
     gene            complement(508..1002)
                     /locus_tag="VFN58_06925"
     CDS             complement(508..1002)
                     /locus_tag="VFN58_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET8917758.1"
                     /translation="MNTRERREFVRSHRIAVFGYKRRDHGPAMTVVYYVMDGDDILVS
                     TMTERAKAKAVGRDPKVSLCILDEKWPPTYLLVYGTATIVTDFEAVVDLRMRIAGLMA
                     EQPIPESNRPDVAETTRREKRVMLRIKPYMTFESPPRHVYQPDDVKGLTHDLGTSLPW
                     NGSS"
     gene            1210..1923
                     /locus_tag="VFN58_06930"
     CDS             1210..1923
                     /locus_tag="VFN58_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8917759.1"
                     /translation="MNQMVFIHGPGAGGCAESFVHQLKRYPGSLAPTLPGHLEGKPCP
                     NVERYTEWLRGWLWSKGKQQDLVLVGFTLGACIALRYGLDYPDEVKALVLMTVAMRPK
                     ERPRSAVELRLKAAEDAETYQKWLDLNRHNMMFIDPDLRERLVECHRKVGPMSQHDDF
                     LVIDQFDVRDRIHTLKPPLLLIRGADDPGSPPEYEMEIHRAVPGSQYLKLNQAGHFPM
                     AEKPVEFNQAIDEFLASRV"
     gene            complement(2073..>2289)
                     /locus_tag="VFN58_06935"
     CDS             complement(2073..>2289)
                     /locus_tag="VFN58_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917760.1"
                     /translation="GKVLSTKVAYHLGHFKRFMTDEEQERKFLPLAEPLLPKGQINDL
                     LACLRRLDEVEQISELISLTVAQKLST"
BASE COUNT          488 a          599 c          655 g          547 t
ORIGIN      
        1 aatcaacata atcgttttgc tcatcgctat tttatcgtgg aggaatactc gccgcgagat
       61 ccagcgcgga ccagtcgcaa ccaaattggg atgactcgcg caaaggcgca aagcatgccc
      121 tgagcaaggt cgaagggggg cgccaagaaa aagggtgtac ccatcggacc tgagctgttg
      181 actcagatca acccttcact tcatgaagca cgaaatgacc gccgtcgctt ttctgggttt
      241 gatagcgcgc ggtcgcttct ctcatggcgt tgtgggtcgg tgtgcagcgg tgccgggtgt
      301 gggcttcgac gttatcccac gcgatcatga taatgacccg gctcgggtct tcgatgctgg
      361 taaaaaagtt aatgccgtgc gagccagcgt agttggcttt cttgagaata tcgccgaaga
      421 ctttgatgta ctcgtcgcgt ttctccggtt cgacatgttg ggtgaggatc tcgtaaatca
      481 tgcgggtctc cttttacaaa tcgtactcta ggacgacccg ttccagggca ggctagtgcc
      541 caggtcgtgg gtcagtcctt tgacgtcgtc tggttgatag acgtgccggg gaggcgactc
      601 aaaggtcatg tacggcttga tccgtagcat gacgcgcttc tcgcgtcgcg tcgtttcggc
      661 gacgtccgga cggttggatt caggaatcgg ctgctccgcc atcagcccgg cgatccgcat
      721 gcgcaaatcg acgacggctt cgaagtcagt cacgatcgtc gcggtgccgt ataccagaag
      781 gtaggtcggc ggccacttct cgtcgaggat gcatagcgac accttgggat cgcgtccgac
      841 tgctttggct ttggctcgtt cggtcatagt cgacaccaag atgtcgtcgc cgtccataac
      901 atagtaaacc acggtcatcg ccggaccgtg gtcacgccgc ttgtagccga agacagcgat
      961 gcgatgcgaa cgaacaaact cacggcgttc tcgagtgttc acgccaattt ctcctccttc
     1021 ctccatcagg ttagttttaa aagcgtcgtt acctggattg gcaacggatg tcaatcttta
     1081 ctgcgaccgt agaagcttga gttatcgata ggataatcgt catcgtttgc tcttgacgct
     1141 cgcgctcgac tgtactacta gccccttcaa aatgccggag acaataagat aaggagacaa
     1201 ggaggggata tgaaccaaat ggttttcatt catgggcccg gggcgggtgg atgtgccgaa
     1261 tcgtttgtcc atcagctcaa acgctatccg gggagcctcg cgcctacgtt gcctgggcat
     1321 ctggagggta aaccgtgtcc caacgtggaa cgatatacgg aatggttgag agggtggtta
     1381 tggtccaaag gtaagcagca ggacttggtg ctggtcggtt tcaccctggg agcttgcatt
     1441 gcgctccgct atggcctgga ctatccggat gaggtcaagg cgctggtatt gatgaccgtg
     1501 gcgatgcggc ccaaggagcg gccgcgcagc gctgtggagc tgcggctcaa ggctgccgag
     1561 gacgccgaga cttatcaaaa gtggctcgac ctcaatcgcc ataacatgat gttcatcgat
     1621 cccgaccttc gcgagcgtct cgtcgagtgc catcgcaagg tggggccgat gagccagcac
     1681 gatgatttcc tcgtgatcga tcaattcgat gtccgggacc gaattcacac cctcaaaccg
     1741 ccgctcctct tgatacgagg ggcggatgat cccggttcac cgcccgagta tgaaatggag
     1801 atccatcggg ccgtacccgg ctctcagtac ctgaagctga accaagccgg tcactttcct
     1861 atggcggaga agcctgtgga atttaaccag gccatcgacg agttcctggc atcccgtgtg
     1921 tgatttccac ttgaaggtga aggctatctc acacgaaggt cacgaaggat tggggaaatt
     1981 attacatttc aatttgttaa cttcgtactc ttcgcgagct tcgtgatgca atgtactgtt
     2041 ggattttggt tgcggcttcg ctgtagctga ggttaggtag acaacttttg cgccaccgtt
     2101 aaggaaatca attcgctgat ctgctcgacc tcgtccaacc ggcgcaagca cgccaggaga
     2161 tcgttaatct gcccctttgg cagcagcggc tctgccagtg gaagaaattt acgctcctgt
     2221 tcctcgtcgg tcatgaaccg tttgaaatgg ccaaggtggt aggccacttt agttgaaaga
     2281 accttacct
//
LOCUS       DASSWZ010000286         5379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1029746, whole genome shotgun sequence.
ACCESSION   DASSWZ010000286 DASSWZ010000000
VERSION     DASSWZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029746"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /gene="ffh"
                     /locus_tag="VFN58_06940"
     CDS             <1..1205
                     /gene="ffh"
                     /locus_tag="VFN58_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET8917761.1"
                     /translation="VVKNFLESVKTKSLGQEVLQSLSPEQQFIKIVRDELIDLLGGEY
                     RELDLKTAPPAVIMLVGLQGSGKTTTLAKLARYLRKDKKRKPYLVPADVYRPAAIDQL
                     KILGEELELPVHDSDPAVSPVALCQQALEAAKQRFCDVLLIDTAGRLHIDEELMQELS
                     SIKNTVHPHQILFVADAMTGQDAVNQAIGFDGKLGLTGIILTKLDGDARGGAALSIRQ
                     MVGKPILFSGVGEKLDALEPFYPDRLASRILGMGDVLSLIDKVQQNVEQKEAVRLQQA
                     FQKQQFTLEEFQLQLQQIKKMGSVGSLLELIPGAKKLASQVDTEKAEQEMKRVEAIIN
                     SMTLEERRNPAILNGRRRLRIAQGSGTTVTDINRLMKQFMEMKKMMQRVSKLGVRSLL
                     GRMPNPFH"
     gene            1224..1469
                     /gene="rpsP"
                     /locus_tag="VFN58_06945"
     CDS             1224..1469
                     /gene="rpsP"
                     /locus_tag="VFN58_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET8917762.1"
                     /translation="MSVRIRLSRHGAKKKPFYRIVVCDQRFPRDGRYIEQVGTYDPNA
                     SAEAVKLNQEKVDIWIKRGAKPTQTVSELMARQKKAS"
     gene            1493..1732
                     /locus_tag="VFN58_06950"
     CDS             1493..1732
                     /locus_tag="VFN58_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET8917763.1"
                     /translation="MMKELVQYLAKSLVNNPDAVEVKETEGETASVLELRVAKEDLGR
                     IIGKQGRTAKSIRTILNAAASRTNRRVVLEIVEEK"
     gene            1751..2263
                     /gene="rimM"
                     /locus_tag="VFN58_06955"
     CDS             1751..2263
                     /gene="rimM"
                     /locus_tag="VFN58_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET8917764.1"
                     /translation="MSERLVPLGEIVTTHGIGGWLKLNPYNPQTTVLNPTREIWLEKD
                     RTRWRHEIERSRPYKSQFLVKLSGVDRIDDAEPWIGSILYLAERDLDSLSSGEYYHYQ
                     VIGLEVFDTKGERIGVVARTWATPGGELYVVNGAFKEHLIPAVKEIIEKVDFAAGKMV
                     INPPEGLLDL"
     gene            2284..3573
                     /gene="trmD"
                     /locus_tag="VFN58_06960"
     CDS             2284..3573
                     /gene="trmD"
                     /locus_tag="VFN58_06960"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET8917765.1"
                     /translation="MPKPMLNFTVVTLFPQMFASPLGHSILKKAQEKGLISIRLVDPR
                     TFATDRHRVTDDYPYGGGQGMVMKPEPLVSAIEDIRAQNPKARVVLLSPQGPVFTQEK
                     AVELAQREDLVLICGRYEGVDERVKSFVDEELSVGDFTLSGGEPAAIVVIDAVARLIP
                     GVLGNEKSAADESFSNGLLEYPQYTRPEDFRGRKVPEVLLSGDHERIKQWRREASFQR
                     TLDRRPDLLAKATLTLEEKAKVNGIKAPIYVALLHHPVYDKNGQVVTTAVTNMDIHDI
                     ARSGRTYGIHGFYVVTPVKALQKLARKIIAHWEQGYGSQYNVTRKEALALVRIKDTLD
                     DVLIDIERECGEKPLLVVTSARPGGSRTSFAELRDMLIKLSQPFLLILGTGWGLTEAI
                     FSQSDYILEAIEGPTDYNHLSVRSAAAIVFDRLLGGQ"
     gene            3592..3948
                     /gene="rplS"
                     /locus_tag="VFN58_06965"
     CDS             3592..3948
                     /gene="rplS"
                     /locus_tag="VFN58_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET8917766.1"
                     /translation="MNILSQIEAEQSKKEPPPLRPGETVRVHVKVVEGEKERTQIFEG
                     IVIALSGKGNRATFTVRKISYGVGVERIFPLHSPRIDKVDVVSRGKVRRAKLYYLRER
                     SGKAARLYGEEGPSGN"
     gene            complement(3918..4322)
                     /locus_tag="VFN58_06970"
     CDS             complement(3918..4322)
                     /locus_tag="VFN58_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET8917767.1"
                     /translation="MAEKLDNGKPPLTQGTPRTVLFPDSSISGKLSYNLPIKIDSRFS
                     GEIKASDLLVVGANAQVDAHISARQLQVEGKLVGNVQVVGCFEIMPGGQFRGEFKVGE
                     LKVHPGAVCEGKGKVLGLVDGVAQLPEGPSSP"
     gene            4403..>5379
                     /locus_tag="VFN58_06975"
     CDS             4403..>5379
                     /locus_tag="VFN58_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET8917768.1"
                     /translation="MDLFELNSSTQASPVQPLAERMRPKTLDEFVGQEHLLGAGKLLR
                     ELTEAGELRSLILWGPPGSGKTTLALILARSAGAVCVHFSAVTSGVKDLKKIIQEAEE
                     FQRLGKRTVLFVDEIHHFNKSQQDNFLPHVERGTLILIGATTENPSFEVISPLLSRCQ
                     VLVLNPLTADDIGKIVDRALLDKQRGLGTWQLEIAPEAREFVIQHSQGDCRVALNALE
                     NAATLARKGRKNKLEVSHLQEALQRKPLQYDKAGDEHYNVISAFIKSLRGSDPDAALY
                     WMMRMIEAGEDPLFIARRMIIFAAEDIGNADPQALQVAVAAKEAFHFVGL"
BASE COUNT         1343 a         1454 c         1425 g         1157 t
ORIGIN      
        1 gggtcgtcaa aaacttcctt gaatcggtca agacgaaatc tctggggcag gaagttttgc
       61 agagtcttag ccctgaacag caattcataa aaatcgttcg agatgagctc attgaccttc
      121 tggggggcga atatcgcgag cttgacctta aaactgctcc tccggcggtc atcatgctcg
      181 ttggcttaca aggctcaggc aaaacgacaa ccttggcaaa gctcgcccgg tatctaagga
      241 aagataaaaa gagaaagccc tatttagttc cggcagacgt ctatcgaccc gccgccattg
      301 accagctcaa aatcctgggc gaggagctgg agctgccggt tcatgattcc gatccggcgg
      361 tctcccctgt ggccctctgc caacaggctt tggaggctgc gaagcaacgt ttttgcgatg
      421 tcctcttgat cgataccgcg ggccgcttgc acatcgacga agaactcatg caggagctgt
      481 cgtcgataaa gaacaccgtc catccccatc aaattctttt cgtcgccgac gccatgaccg
      541 gtcaagatgc tgtgaaccag gccatcggat tcgacggcaa acttggtctc acaggaatta
      601 ttctcactaa attggacggc gatgcccgcg gcggtgccgc tctttcgatt cgacagatgg
      661 ttggcaagcc gattctcttc tccggtgtgg gcgagaagct tgatgcactc gagcccttct
      721 atcccgatcg tctggcgtcg agaatcctcg gcatggggga tgtactttcg ctcatcgaca
      781 aggttcagca gaacgtcgag cagaaagaag cggtccgtct acagcaggcc tttcaaaaac
      841 agcaattcac cttggaagag tttcaacttc agctccaaca gatcaaaaaa atgggctcgg
      901 tcggtagcct gcttgaactc attcccggag ccaagaaact tgcatcgcag gttgacacag
      961 aaaaggccga gcaagagatg aaacgcgtag aagccatcat caactcaatg accctcgaag
     1021 agcgacgcaa tccggcgatt ctgaacggcc gccgacgcct gcggattgcc caggggagcg
     1081 gcaccacggt tacggatatc aatcggttaa tgaaacagtt catggagatg aaaaagatga
     1141 tgcaaagggt gagcaagctg ggagtgcgat cactgctcgg ccgtatgccc aaccctttcc
     1201 attagaggag aaggaacgat aatatgagtg taagaattcg cctcagccgg cacggggcaa
     1261 agaaaaagcc cttttatcgt atcgtcgtct gcgatcagcg ctttccgcgt gacggtcgat
     1321 acatcgaaca ggttggaaca tacgacccta acgccagcgc cgaagccgtc aaactgaacc
     1381 aagaaaaggt cgatatctgg atcaagcgtg gcgccaaacc cacacagacc gtgtccgagc
     1441 tgatggcgcg ccagaagaaa gcaagctaat ctccggatag gaggtcacct ccttgatgaa
     1501 ggagctcgtt caatacctgg caaagtcctt ggtgaacaat cccgatgctg tcgaggtcaa
     1561 agagaccgaa ggagaaaccg cctcggtttt ggaattaaga gtggccaaag aagatctggg
     1621 acggatcatc ggcaagcaag gtagaacggc aaaatccatc cgcacgattc tcaacgccgc
     1681 agcgtcaaga accaaccgca gggtggtcct tgaaatcgtc gaagagaagt agccttcctg
     1741 cgtcagaccc gtgtccgagc ggctcgtccc tcttggagag atagtcacca cccatggtat
     1801 cggtggctgg ctgaaactca acccgtacaa ccctcagaca accgttctta acccaacccg
     1861 ggagatttgg ttggagaaag accggacgcg atggaggcat gaaatcgaaa ggagccggcc
     1921 ttacaagagt caatttctag taaaattatc cggagtggat cggattgatg acgccgaacc
     1981 atggatcggt tctatcctct atctagcgga gcgggatctt gattctctgt catcgggtga
     2041 atactaccac tatcaggtga tcggcctgga ggttttcgat accaaaggag agcgaatcgg
     2101 ggtcgtcgct cgaacttggg ccacgccagg cggagagctg tatgtcgtaa atggcgcttt
     2161 taaagaacac ctcattcctg cggtaaaaga aatcatcgag aaggtcgatt tcgccgccgg
     2221 gaagatggtc atcaatcccc ctgaagggct gctggatctt tagtcctcgc attcgatgcg
     2281 agcgtgccca aaccgatgct taattttacg gttgtcacct tattcccgca aatgtttgca
     2341 tccccgctgg gccacagcat cctcaagaag gcccaggaga agggtctgat ctccattcga
     2401 ctggtggatc cgcgcacctt tgccactgac cgacatcgtg tcaccgacga ttatccctac
     2461 ggtggaggcc aggggatggt gatgaagccg gaacccctag tgtcggcgat cgaagatatt
     2521 cgcgcgcaaa atcccaaagc aagagtcgta ttgctttcac cccaaggacc agtcttcaca
     2581 caagaaaaag ccgtcgaatt agcacaaaga gaagatttgg ttctgatctg cggccgctat
     2641 gaaggtgtcg atgaacgggt taaatcattt gtcgacgaag aactttccgt gggcgacttt
     2701 actttgagcg gaggtgaacc cgccgctata gtcgtgatcg acgctgtcgc ccggttgatc
     2761 ccgggtgtct tgggaaatga aaagtctgcc gcggacgaat ctttcagcaa tgggctgttg
     2821 gaatacccac agtacactcg ccctgaagat tttcgcggca ggaaggttcc agaggtactt
     2881 ctttcaggtg atcatgaacg cataaagcag tggcgacggg aagcgagttt ccagcgaacc
     2941 ctggaccggc gtcccgatct gcttgccaaa gcgactctga ctctggaaga gaaagccaag
     3001 gtaaatggaa tcaaggcgcc catctatgtc gcgctactcc atcacccggt ctacgataag
     3061 aatggccaag tcgtaaccac agccgtgacc aacatggaca ttcatgatat tgcccgctcg
     3121 ggaaggacct acgggattca cggcttctat gtagttacac cggtcaaggc cctgcaaaaa
     3181 ctcgcccgca agataatcgc ccattgggag cagggctatg gcagtcagta caacgtcact
     3241 cgcaaggagg cgctagctct ggttcgcatc aaagataccc tggacgacgt gctgattgat
     3301 atcgagcggg aatgtggaga gaaacctttg ttggtcgtca cgtccgccag accaggcggc
     3361 agtcgcacct ctttcgcgga gctccgagac atgttaataa agttaagtca gccgttcctg
     3421 ctgattctgg gcacgggatg gggactcaca gaagccattt tctcacagtc agactatata
     3481 ctcgaagcaa ttgaggggcc gactgactat aatcatcttt cggtgagatc ggccgcagcg
     3541 atcgtgttcg accgacttct aggcggtcag taaatttcac aggagttagc catgaatatc
     3601 ctaagtcaaa ttgaagcgga acaatccaag aaagagcccc cccccttgcg accgggtgag
     3661 accgttcggg ttcacgtgaa agtagtagag ggcgaaaaag agcgaactca gattttcgag
     3721 ggaatcgtca tcgctctttc cggcaaaggc aaccgcgcga ccttcactgt ccgaaaaatc
     3781 tcctacggcg ttggcgtgga gcggatcttt cccctgcact cgcctcgcat tgataaggtt
     3841 gacgttgtat cgcgcggcaa ggtacgccgc gccaaacttt attatctgcg tgaacggtcc
     3901 ggcaaggcag cgcgtctcta tggcgaggag ggaccttccg gcaactgagc aactccgtcc
     3961 actagaccca ggacttttcc tttcccttcg cacacggctc cagggtgaac cttgagctcc
     4021 ccgactttaa attcaccccg gaattggccg cccggcataa tttcgaagca accgaccacc
     4081 tgtacgttgc ccaccaactt cccttcgact tgaagttggc gcgcagagat atgggcatcg
     4141 acctgggcgt tcgcgcccac caccaaaaga tcactggcct tgatttcccc actgaagcgg
     4201 ctatcgatct ttattggcaa attgtaactg agttttcctg atattgagga gtcgggaaaa
     4261 agcacagtcc tcggggtgcc ttgcgttaaa ggtggcttgc cgttgtccaa tttttccgcc
     4321 atgccttaca acctcaaaac cgccgacgta gagcccctga gcctgttgta ccctagccgt
     4381 tttgatatga tagcatgaaa tcatggatct attcgaatta aattcatcca cccaggccag
     4441 tcccgtgcaa ccattagccg aacgcatgcg gccgaaaacc ttggatgaat ttgtcgggca
     4501 agagcatctg ctcggcgccg gcaagctgct tcgcgaactg accgaggcag gcgagcttcg
     4561 atcgctcatt ttgtgggggc caccgggcag cggcaaaacc accctggcct tgatcctggc
     4621 gcgctccgcc ggcgcagtct gcgttcattt ctcagccgtc acctccgggg tcaaagatct
     4681 caagaagatc attcaagagg cagaggagtt tcagcgtctc ggcaaacgca ccgtgctttt
     4741 tgtcgacgag attcaccact tcaacaaatc ccaacaggat aacttcttgc cccacgtgga
     4801 acgcggaacc ttgatcttga tcggtgccac cacagagaat ccatcttttg aagtgatctc
     4861 gccactgctt tcgcgctgtc aggttttagt tttgaatccg cttaccgccg atgacatcgg
     4921 aaaaatcgtc gatcgcgcgc ttttggataa gcaacgggga ctgggaacat ggcagcttga
     4981 gattgctcct gaggcacgtg agtttgtcat tcaacactct cagggggatt gccgcgtagc
     5041 gctaaacgca ctggaaaacg ccgcgacctt ggcccgcaag ggtagaaaaa acaaactcga
     5101 agtaagccac ttacaagaag ccttgcagcg caagccgctg caatacgaca aggccggcga
     5161 tgaacactac aacgtgattt cggccttcat caaaagtctt cgcggcagtg acccagatgc
     5221 cgccctctac tggatgatgc gaatgattga ggccggagaa gatccccttt tcattgcccg
     5281 tcgcatgatc atctttgcgg cggaagatat cggcaacgcc gatccgcaag cgctccaagt
     5341 cgccgtggca gccaaggaag cctttcactt tgttggcct
//
LOCUS       DASSWZ010000287         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_294234, whole genome shotgun sequence.
ACCESSION   DASSWZ010000287 DASSWZ010000000
VERSION     DASSWZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294234"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1100)
                     /locus_tag="VFN58_06980"
     CDS             complement(132..1100)
                     /locus_tag="VFN58_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8917769.1"
                     /translation="MTLTKPIKGIIGACLTPFDDNDQINYKALEKEIDFIVSDCDAIT
                     IAAVEAAEYTMLSRDERKEILRVATEMVNKRVPVILGCSSPSPREVIELAEYSAKVGG
                     DLVQVLMPLRPWGGQPTIAELMEFYTQVASASPLPVTCYHNPGPGADPPQDAFVKISE
                     IDNIRYFKESSRDITKISRLIEQIDLAGRGHYFTTMQPLLVTLMLGGSGATMPPPGTR
                     IGAQIVRAFRAGDLDTARFWARCYALFPGKWAAYGLPPVMKSAMKHFGIDIGNPCRPY
                     APVSPRDHAQIGQFLQQVGLIGEAGPTPQALLDAGEKLRQEDTFLR"
     gene            complement(1106..1762)
                     /locus_tag="VFN58_06985"
     CDS             complement(1106..1762)
                     /locus_tag="VFN58_06985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET8917770.1"
                     /translation="MEMVDLSRVIYDGMPKIPVLPDVHVQKFFSLEKGHPLNVTELSL
                     PCHAGTHVDAPIHIVPNGKSIEELPLDAFVGAGAVIGVKKKGGEEVTAKDLEDSGVAV
                     SRGDILMLYTGWDEKFESPDYNVHPYLSVDAAEWMVKKGIKMFGIDCITVDLPTPLRP
                     KGFDFPVHRTLLGNNVLIAENVANLGSIVGKRTRIIALPLRVKGSDAGHARIVAEILN
                     "
     gene            complement(1775..2515)
                     /locus_tag="VFN58_06990"
     CDS             complement(1775..2515)
                     /locus_tag="VFN58_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET8917771.1"
                     /translation="MGISSAEFVKEIQQMVRKKHSKDHPIVGMIEQGKLDRQQLKGFV
                     GQFYLFFPKPFPKPIAAMLSRCPDDSELEKMWIENVVEEGTGEITGTDSHKGLFIRFA
                     GACGYSKEELDRVEPLPETEAFLDWRELLMYQRSWLELYACQGFCLEGTANERMTRIV
                     NGLTKHYGFDRNSEDIRYWTLHMGVDEEHMKVGPLAVERYAVTDPQQVQVRASVQKTL
                     DQFWLAFDGIKRAFVDKDPIYARWRNGK"
     gene            2754..>3694
                     /locus_tag="VFN58_06995"
     CDS             2754..>3694
                     /locus_tag="VFN58_06995"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HET8917772.1"
                     /translation="MRINFFPSSHLMFLSLFLSLTSALATVRLVEAQPSAPDVVRFLE
                     QSTFGPTPELIARVRQAGFEAFLDEQLAAPISDYLELPFWPQTRPTSCTGDCQRDNYT
                     YYQLQRHFFTNALYGQDQLRQRVAFALGQILVTSQVDIPLPSWMRGYQQLLYRSAFGN
                     FRQLLYDVTLNPTMGRFLDMLNNRCQTRTPPDISICRNGLNAQPNENYAREILQLFSI
                     GTFLLNQDGTRQLDANGNPIPTYDQKIVEEFARVFTGWILAPALSGPSGIGGTVPNYR
                     DPMRVRVSGGFEDYHDKGPKTLLNGLQLPGGQSQAVEL"
BASE COUNT          841 a         1046 c          891 g          916 t
ORIGIN      
        1 tctgccgtcg cgagtcagct ccttgccaaa tcccgaggtt gcgcgcaaat accaaattag
       61 tagtttatcc gaaagatgct taatccattc cataaatagg tttccaaaac ggggaggacg
      121 gcagcgaatc gctagcgaag gaaagtgtct tcctggcgaa gcttctcacc cgcgtccagc
      181 aacgcttgag gtgtcggtcc cgcttcacca attagaccca cttgttggag aaattgcccg
      241 atctgtgcat gatcacgggg gctgacgggg gcataagggc ggcaaggatt tccgatgtcg
      301 ataccgaaat gcttcatcgc cgatttcatc acgggcggta aaccgtaggc tgcccatttg
      361 cccggaaata gtgcataaca gcgcgcccag aagcgcgccg tatcgagatc ccccgccctg
      421 aatgctctaa caatctgtgc gccaattctg gtaccgggtg gcggcatcgt cgcacccgac
      481 ccaccgagca tcagagtcac cagcaaaggt tgcatggtgg taaagtaatg accacgccca
      541 gctagatcga tttgctcaat caatctggat atctttgtaa tatcccgcga gctttccttg
      601 aagtaacgaa tattgtcgat ttcgctgatt ttgacgaagg catcctgcgg cggatcggcg
      661 ccggggccgg ggttgtggta acaggttacc ggcagcgggc tcgcggaggc gacttgagta
      721 tagaactcca tcaactcggc aatggtcggc tgccctcccc atggccgaag cggcatcaga
      781 acttgcacca gatccccgcc caccttcgca gagtattccg ccaattcgat gacctctcta
      841 ggagacgggc tcgagcaacc cagtatgacc ggcacgcgct tgttgaccat ctcagtggca
      901 acccgcaaga tttccttccg ctcatcgcgg gacagcatgg tatattcggc cgcttctaca
      961 gcagcaatgg tgatggcgtc acagtcggaa acaataaaat cgatttcttt ctccaacgct
     1021 ttgtaattta tctgatcgtt gtcatcgaac ggtgtaaggc aagcgccgat aatgcctttg
     1081 atgggttttg tgagtgtcat agttgtcagt tcaagatttc cgccacaatc ctagcgtggc
     1141 cggcatcact gcccttcact cgcagcggca gagcaataat tcgggtcctt tttccgacaa
     1201 tgcttcccag atttgcgaca ttctccgcga tcaagacgtt gttcccaagt aacgttcggt
     1261 gaaccgggaa atcaaagccc ttgggccgga gcggcgtcgg aaggtccact gtaatgcagt
     1321 cgatgccaaa catcttgatg ccttttttta ccatccattc cgcagcatcc actgagagat
     1381 aaggatgcac gttgtagtcc gggctttcga atttttcatc ccaacccgta taaagcatta
     1441 gaatgtcccc gcggctgact gctacgcccg aatcttccaa gtccttggcg gtcacttctt
     1501 ccccaccttt ttttttcaca ccgatcaccg ctccagcacc aacaaaggca tcgagcggca
     1561 gttcttcgat ggattttccg ttcggcacga tatgaatcgg cgcgtccacg tgagtgcctg
     1621 cgtgacaggg tagcgaaagc tcggttacat tcaggggatg acccttctcg agactgaaaa
     1681 atttctgcac gtgcacgtcc ggaagcacgg gaattttagg cattccgtca taaatcaccc
     1741 gtgaaagatc aaccatttcc atagaaaacc ctccttactt tccgtttcgc cacctggcgt
     1801 aaatagggtc cttgtctaca aaggctcgct tgattccgtc aaacgccaac caaaactggt
     1861 cgagtgtctt ctgcacggac gcccgaacct gcacttgttg cggatctgtt acggcatagc
     1921 gctccaccgc cagcggccct actttcatgt gttcttcgtc gacccccata tgcaaagtcc
     1981 aataacggat atcctccgag ttgcgatcga atccatagtg tttcgttagc ccgttgacga
     2041 ttcgtgtcat ccgttcgttg gcagttccct cgaggcaaaa tccctggcag gcgtaaagtt
     2101 caagccaaga ccgttggtac atcaacaact ctcgccaatc cagaaaggct tcggtctcag
     2161 gcaacggttc aacccggtcc aattcttctt tgctatatcc acaggccccg gcaaaacgaa
     2221 tgaataagcc tttatgacta tcggttccgg tgatctctcc tgttccctcc tctaccacgt
     2281 tctcgatcca catcttttca agctcggaat cgtccgggca acggctcagc atcgctgcga
     2341 tgggcttggg gaaaggcttc ggaaagaaga gatagaattg cccgacaaat cctttcagtt
     2401 gttgtcggtc gagcttgcct tgttcgatca ttccgacaat cggatggtcc ttggagtgct
     2461 ttttgcgaac catttgttga atttctttga cgaattccgc gcttgaaatt cccataacct
     2521 cttctcccac tctcgctaaa ttatttgaac gcttatcaca tgcaacgatt tgaagccacc
     2581 ggttcaacct atctcaccaa ggtttcagag cgtaaacaaa ctaatcgtcg attattttct
     2641 tgacagccac tctttagtct tagtatacct ccgcctgccg aatttgaaat cagtcagttt
     2701 aatcctttat cgcgggcatc atcttacagc gatgaattct aggaggtcgc atcgtgagga
     2761 taaatttttt cccgtcgtct cacctaatgt tcttatcgct tttcctttcg ttgacttccg
     2821 cgttggctac ggtgcgtctg gttgaagcac agccatccgc gccggacgtg gtacgtttcc
     2881 ttgaacaatc gacattcggt ccgacgcctg agctgatcgc ccgcgtgcgg caggccggct
     2941 tcgaagcatt cttggatgaa cagctggcgg caccaatctc ggattacctg gaattgccgt
     3001 tttggccgca gacgcgtccc acaagctgca ccggagactg ccaaagagac aactatacgt
     3061 attaccaact ccagcgccat ttcttcacca atgccctcta tggtcaagac caattgcgcc
     3121 agagggtcgc ttttgcgttg ggccagatac tggttacctc ccaggtagat attccccttc
     3181 caagctggat gcgaggctat cagcaactgc tctatcgaag cgcctttggc aactttcgcc
     3241 aattgctcta cgatgtgact ctgaatccga cgatgggtcg cttcctcgac atgttgaaca
     3301 accgctgcca gacccgaacg ccgccggata tcagcatttg tcgaaacggc cttaacgccc
     3361 aacccaatga aaattacgcg cgcgagatct tgcagctttt ctcaatcggc accttcctgc
     3421 tgaatcaaga cggtactcga caattggatg ccaacggcaa ccctattccc acctatgatc
     3481 aaaagatcgt agaggagttc gccagggtat tcaccggttg gattcttgcc cccgcattgt
     3541 cgggtccttc gggcatcggc gggaccgtac cgaactacag agatccgatg cgagtgcggg
     3601 taagcggagg tttcgaggac tatcacgata aggggcccaa gacgctgctc aatggattgc
     3661 agctgccagg cgggcagtca caagcggtgg aact
//
LOCUS       DASSWZ010000288         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1115218, whole genome shotgun sequence.
ACCESSION   DASSWZ010000288 DASSWZ010000000
VERSION     DASSWZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115218"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1229)
                     /locus_tag="VFN58_07000"
     CDS             complement(183..1229)
                     /locus_tag="VFN58_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HET8917773.1"
                     /translation="MTILKIFFLTVFMALFSLIVRQDDLTNAQDGFFSGKIVRIVVGS
                     SPGGGYDYWARLLARHMPKYIPGNPEVVVQNMPGGGSLVATNYVYSVAKPDGLTVGMP
                     NQLVYMGQVVGDKEARFDVQKFNWIGSPDRNPTVLFIRADTPYKSIDDIIKAKIPPKC
                     GGSGRDSSSMIFALEDLVGAKFEVVLGYQGGSQTDLAIERGEVVCRSMGLGAHFSRQP
                     FTLWHSKDFDRHLVQTGRRRDDRASDTPTLYELMDRYKTADLGRRLAGVLTAGEDFGH
                     PMMAPPGVPAERVKLLRDAYAKTLKDPELLADVKKQEYDFDPVSGDELQTLAKTVVNQ
                     PAQVIERLKKILGN"
     gene            complement(1250..1876)
                     /locus_tag="VFN58_07005"
     CDS             complement(1250..1876)
                     /locus_tag="VFN58_07005"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET8917774.1"
                     /translation="MAYAPVRKVDVPYEHWDRLYSPVACAVMITTVDKEGRVNAGSFA
                     TCVRQNHEPTCISFTVEIDKDTYHNVLATEEFVVNIPSFDREILEKVRVVGLPFARGV
                     NELEKAGLTAIPGRALRPPRIAECKAHYECKVIWTKEWLHRLTVMGQVVAGSVDEDCI
                     DKDGYAILEKLRPTHYCGRAFDNKFVASYEVMEVDMVYQGPEAQHKPK"
     gene            2060..2869
                     /locus_tag="VFN58_07010"
     CDS             2060..2869
                     /locus_tag="VFN58_07010"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET8917775.1"
                     /translation="MLTVKNVSKVFNTLDNGVQSVRALDDVSLDVKEGEFFSIIGPSG
                     CGKSTLIRIIAGLVPTYDGEVRIGTERVTAPHPWVGMVFQEESTFPWRTTVGNVEFGL
                     EMRDVPPAARRTKALEMLDLVGLGGFEHRYPSELSGGMKQRVAIARALVMEPKILLMD
                     EPFGALDEQTRIILGEELLKIRDKLRQTIVFVTHNINESVQLSNRVMVMTARPGRVKA
                     VFRIDLPHPRDSTIIASDKFGQLVGQVWGTLRDESLKGFKQMEKTDQGRRS"
     gene            2866..>3524
                     /locus_tag="VFN58_07015"
     CDS             2866..>3524
                     /locus_tag="VFN58_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917776.1"
                     /translation="MKKVTFVLSTLLVLGYFALPAAQAAAGPTKIILVVPHRVFFTVA
                     VPVYIAQEKGYYKEGNIDVDAVFTKGGGENVQAVVSGDAQIGLSTGFFAVLSAFSKGA
                     PIKVAAAEITGMDSFWYAPANTPVRKMEDLAGKKVAFSRAGSSTHMAVLAMSDQIKAK
                     GLKPLEPVSLGGVPESYTGVRTGQADAGWSVAPFMLDRIDKGELRVVVKGSEIEALNA
                     IT"
BASE COUNT          816 a          959 c          915 g          834 t
ORIGIN      
        1 cccacggccc gataccggcg gctttaaccc gcacaacgac ctctcccctg cccggctccg
       61 gcttgggtaa atcatcgatg gagatgactt ccggcgaacc aaacttgtgg actcgaacag
      121 ccttcattcc tttcaccctc cccgattggg gacgaacgct ccagcttgta gccggtagtg
      181 agtcagttac ccagaatctt cttgagcctt tcaatcactt gagcgggttg gttgacgacc
      241 gtctttgcaa gggtttgaag ttcatcaccc gagaccggat caaaatcata ttcctgcttc
      301 ttaacatcag ccaaaagttc agggtctttt agtgttttgg cgtaagcgtc gcgaagaagc
      361 ttcactctct cagcgggcac gccgggcgga gccatcatag gatggccaaa atcctctcct
      421 gcggttagaa cgcccgccaa tcgacgtcct aagtcagcgg tcttgtagcg atccataagt
      481 tcatagaggg tgggagtatc cgacgcccgg tcgtcccgtc ttcgcccggt ttgaactaga
      541 tgccgatcaa aatctttgct atgccagaga gtaaaaggct gcctgctgaa atgtgctccg
      601 aggcccatcg agcggcagac aacttctccc ctttcaatcg caagatcggt ttgactgcct
      661 ccttgatagc ccaacaccac ttcaaatttg gcgccgacta gatcctccag agcgaagatc
      721 atgcttgaac tgtctctccc ggaacctcca catttcggag gaatcttggc tttgatgatg
      781 tcgtcaattg atttgtatgg cgtgtcagcc ctaatgaaca agaccgtggg attacgatct
      841 ggcgagccta tccagttgaa cttttgaaca tcgaatctcg cttctttgtc gccaacaact
      901 tggcccatgt aaactaactg attcggcatg cctacggtca ggccatcggg tttcgccaca
      961 ctgtaaacgt aattggtggc aaccaacgat ccgccgcctg gcatattctg aacgacgact
     1021 tcaggattcc caggtatgta tttcggcatg tggcgggcca gaagccgtgc ccagtaatcg
     1081 taacctcccc ctgggctcga cccaacgacg attcgaacaa tctttccgga aaaaaatccg
     1141 tcttgcgcat tggttagatc gtcttgccta acgatcaatg aaaacagcgc cataaaaacc
     1201 gttaagaaaa agatcttcaa aatagtcatt gcgatcccaa cggcaaatcc tacttcggct
     1261 tatgttgcgc ctcgggtcct tggtacacca tatcgacttc catgacttcg taagaggcga
     1321 caaatttgtt gtcaaaagcc ctgccgcaat agtgcgtagg gcggagtttt tcgagaattg
     1381 catagccgtc cttgtcgatg cagtcctcat cgacagagcc cgccaccact tgtcccatca
     1441 ccgtcagacg atgcagccac tctttggtcc atatgacttt acactcgtag tgagctttac
     1501 attccgctat gcgaggcggc ctgagagccc tacccgggat agccgtgagg ccggcttttt
     1561 ccaactcgtt gactccacgg gcgaagggca gtcccaccac tctcaccttt tcaagaattt
     1621 ccctatcaaa ggacggaatg ttgacaacga attcttcagt agccagcacg ttgtgatacg
     1681 tgtctttatc tatctccacc gtgaacgaaa tgcaggtcgg ttcatgattc tgtctcacac
     1741 aggtggcaaa tgaacccgca ttcactcgcc cttccttatc gaccgtagtt atcatgaccg
     1801 cgcaagctac cggagaatat agtcggtccc aatgttcata cggaacgtca acctttcgaa
     1861 ctggtgcgta agccataact ttttactcct tcactttgtc ttcgcggcaa cgtacacaag
     1921 ttaaaaaatt gtgtcaaagt ctcctcagcc tgtctcatga tcgccttctc cgatacgttc
     1981 atagtacgcc ggctttgttt gtgctattta gcacatcgag accgaggagc cgaggctcct
     2041 ccgacagaca tgctccgtca tgttaacggt caaaaacgtc agcaaagtat ttaacacgct
     2101 ggataatggc gtacagagcg tcagggccct tgacgatgtc tcgctggacg tcaaagaggg
     2161 agaatttttt tccattatcg gtccgtcggg atgcgggaaa tctactttga taaggattat
     2221 tgccgggctc gtcccaacct acgacggcga ggtaaggatc ggaacagaac gggtgactgc
     2281 gcctcatccg tgggtcggga tggttttcca ggaagaatcg acttttccgt ggcggacgac
     2341 agtaggtaat gtcgaatttg gtcttgagat gcgcgacgtt ccgcccgcag cgcgaagaac
     2401 caaggctctt gagatgctcg accttgtggg tttaggggga tttgaacacc gctacccttc
     2461 tgagctgtcc ggcggcatga agcagcgggt cgccattgcg cgcgccctgg tgatggaacc
     2521 taaaattctt ctgatggacg agcccttcgg cgcgctcgac gagcagacgc ggatcattct
     2581 tggtgaagag ctcctcaaga tccgtgacaa actcaggcag acgattgtct ttgttaccca
     2641 taatatcaat gaatcagtgc agctttcgaa tcgcgtcatg gtgatgacgg ctaggccggg
     2701 gcgagtcaaa gccgttttca gaatcgacct gccgcatccg cgcgattcga cgattattgc
     2761 atccgacaag ttcggccagc tcgtaggaca ggtttgggga acactcaggg atgaatcact
     2821 caaaggcttc aagcagatgg aaaaaaccga ccaggggagg cgctcatgaa aaaagttacc
     2881 ttcgtcttga gtacgctgtt ggtgcttgga tatttcgctc taccggccgc gcaagccgca
     2941 gctggaccaa ctaaaattat cctcgtcgta ccgcacaggg tgttcttcac tgtggcggtt
     3001 ccggtctata tcgctcagga aaaaggttac tacaaggagg gtaacatcga cgtcgatgct
     3061 gttttcacaa aaggcggcgg cgagaacgtg caggcggtgg tatcgggaga tgcgcaaatc
     3121 ggtctatcga ccgggttctt cgcggtcctc agcgccttta gcaaaggcgc gccgatcaaa
     3181 gtcgcggctg cggagataac cggcatggat tccttttggt acgcgccggc gaacacgccg
     3241 gtccggaaaa tggaagatct ggcggggaaa aaggtggctt tcagccgcgc cggctcgtcg
     3301 acccacatgg ctgtgctagc gatgtcggat caaatcaaag cgaaaggact gaaacccttg
     3361 gagcccgtct ctctgggcgg cgtacccgaa agctataccg gcgttcgcac cggccaggcg
     3421 gatgccggtt ggtccgtggc gccattcatg ctggaccgca tcgacaaggg cgagctgcgc
     3481 gtcgtggtga agggttccga aatcgaggcg ctcaatgcga tcac
//
LOCUS       DASSWZ010000289         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_704590, whole genome shotgun sequence.
ACCESSION   DASSWZ010000289 DASSWZ010000000
VERSION     DASSWZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704590"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFN58_07020"
     CDS             <1..189
                     /locus_tag="VFN58_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917777.1"
                     /translation="ILTEAELPTITASQVLVRLAENGSRSRLLCRLTSLLKWRRQWER
                     SLWKWMISPDTQDRSEGA"
     gene            195..875
                     /gene="rpiA"
                     /locus_tag="VFN58_07025"
     CDS             195..875
                     /gene="rpiA"
                     /locus_tag="VFN58_07025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET8917778.1"
                     /translation="MGSTDYAKELAARKSLDFIEDGMVVGLGTGSTASHFIKLLGERV
                     KRGLKIRAVPSSNTSKELAESLAIPIIDFHRCSEIDVAIDGADEIAPGLALIKGGGGA
                     LLHEKIVASASRRFIVIADSSKVVTRLGKVPLPVEVIPIASPLVTRKLVEMGIRPTIR
                     RLSAGSEFITDEGNLILDCDCGEIQDPDGLAASIRNIVGVVEHGLFLHMAERALVSDG
                     SEVTTLFK"
     gene            954..1349
                     /locus_tag="VFN58_07030"
     CDS             954..1349
                     /locus_tag="VFN58_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HET8917779.1"
                     /translation="MTVNRGRGIIDTPSNHSVEDTVEKLKGILQAKGVTLFALVDHSA
                     EGEKVGMKMPPTKLLIFGSPKAGTPVMLAAPSIAIDLPLKILIWEDREGKVWVSYNSP
                     TYLQERHGLAYELLQNIAAVETLAAAVAE"
     gene            1770..2603
                     /locus_tag="VFN58_07035"
     CDS             1770..2603
                     /locus_tag="VFN58_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8917780.1"
                     /translation="MAYITVGKENSADIEIYYKDWGSGQPVVFSHGWPLNADAFEDQM
                     YYLASRGYRCIAHDRRGHGRSSQPWNGNDMDTYADDLAKLVEALDLKDAIHVGHSTGG
                     GEVARYIGRHGTKRVAKAVLIGAVPPLMLKTPANSGGLPMEVFDQIRAGVVGDRSQFW
                     KDLSLPFYGYNRPGAKISEGVRESFWLQGMMCGFPGSYFCIKAFSETDQTEDLKKFDV
                     PTLILHGDDDQIVPIGAAAMLSSKLVKGAVLKVYKGAPHGMCTTLKDQVNEELLGFFK
                     A"
     gene            2689..>3008
                     /locus_tag="VFN58_07040"
     CDS             2689..>3008
                     /locus_tag="VFN58_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET8917781.1"
                     /translation="MVTKTATPTDLSGNAVEEISSNLRRLLADVFALYVKTKNFHWHM
                     SGQHFRDYHLLLDEQATQIFAMTDDIAERARKIGGTTIRSISDIAKNQRLKDNNKEGV
                     TPQE"
BASE COUNT          729 a          793 c          854 g          632 t
ORIGIN      
        1 atcctcactg aagctgagct tcctacgatc accgccagcc aggtactcgt tcgcttagcc
       61 gagaacggtt cacgttcgcg gctgctctgc aggctcacgt cgctgttaaa atggcgaaga
      121 cagtgggaaa gatcgttgtg gaaatggatg attagccccg atacgcagga tcgctcagaa
      181 ggtgcatgac gcccatgggt tccacggact acgcgaagga gttggctgcc aggaagagcc
      241 tggattttat tgaggacggc atggtggtcg gcctcgggac aggctcgacc gcaagccact
      301 tcatcaaatt gctgggggag cgcgtgaagc gcggtctgaa aattcgcgcc gtcccttcat
      361 cgaacacgag caaggaactt gccgaatccc ttgcaatccc aattatagat tttcaccggt
      421 gttcagaaat tgatgtggcg attgacggag cggatgagat cgctccgggg ctggcgctga
      481 tcaagggtgg aggaggtgct ctgttgcacg agaaaattgt cgccagcgcc tcaagaagat
      541 tcatcgtaat tgccgattcc agtaaagtcg tcacccgcct cggaaaggtt ccactgccgg
      601 ttgaagtgat tcccatcgca tctcctctgg tgaccagaaa actagtggaa atgggaatcc
      661 gcccaacgat caggcggtta agcgccggct ctgagttcat caccgacgaa ggaaatctga
      721 ttctcgattg cgactgcggt gaaatccagg atcccgatgg cctggccgca tccattcgga
      781 atattgtcgg ggttgtggag cacggtcttt ttctacacat ggcagagcgg gccctggttt
      841 cggatggctc cgaagtgacg accctattca aatgaggtct gtgtggatcg ttctttgagt
      901 aagaggcaac aaaatggatc ggcaacccgg aaatcaataa tgaggaggtg tctatgacgg
      961 tcaatagagg caggggaatc atcgacacac ccagcaatca ctcggtggag gatacggtcg
     1021 agaagctcaa agggattctg caagccaagg gagttaccct cttcgcgctg gtcgatcaca
     1081 gcgcagaagg ggaaaaggta ggaatgaaaa tgcctcccac aaagctgctg attttcggga
     1141 gcccgaaggc aggcactccc gtgatgctgg cggcgcccag tatcgccatc gacctccccc
     1201 ttaagatttt gatctgggag gatcgcgaag gtaaggtctg ggtctcctat aacagcccga
     1261 cgtacttgca ggagcgacac ggcttggctt acgaattact gcaaaacata gctgcagtcg
     1321 aaaccttggc agccgctgtc gcggagtaat tctgggaatg catcgtgttt tcaccagagc
     1381 ccctaacgag ccaatgacac acattcccct tcaacaatgg tttctgaacg atctctgtta
     1441 tggcctcaac atctttctcg tcttgattgc tagttggtcg gcgggtctaa agcgccgccg
     1501 ctcggcttgg aatgaagcgc acgcatcgtg attatccggt gaagaggcag ggattgatca
     1561 gccaagcacg agacgatatc tcgtcatctg acggcaagcg gtgacggctt ttcgtcacaa
     1621 ttctacgaaa ctcaaatgta acattggaaa ctcttctgtt tccctaacta tgaatgattc
     1681 ccaaacacga tccgtctggc acaaagcttg ccggtttcat gaaagaaccc atctgaatac
     1741 atttcaaatt tcaccaaagg agattttcaa tggcttacat taccgtcggt aaggagaatt
     1801 ccgcggacat cgagatttat tataaggact ggggcagcgg gcagcccgtg gtcttcagcc
     1861 acggctggcc gctcaacgcg gacgcctttg aggaccagat gtactatctg gcctcccgcg
     1921 gctaccgctg catcgcccat gaccgccgcg gccatggccg ttcgagccag ccctggaacg
     1981 gcaacgacat ggatacctac gcggatgacc tggcgaagct ggtagaagcc ctcgatctaa
     2041 aagatgcgat ccacgttggc cattcgaccg gcggcggtga agtcgcccgc tatatcggcc
     2101 gtcacggcac caagcgtgtg gccaaggcgg tgctgatcgg agcggtgccg ccgctgatgc
     2161 tgaaaacgcc cgccaattcg ggaggcttac cgatggaggt gtttgaccag atccgcgctg
     2221 gcgttgtagg cgaccgctcg cagttctgga aggatctcag cctgccgttc tacggctaca
     2281 accggccagg ggcaaagatc tcggaagggg tgcgcgaatc cttctggctt caggggatga
     2341 tgtgcggttt tccaggctct tatttttgca tcaaggcgtt ttccgagacg gatcagaccg
     2401 aggatctcaa gaaattcgac gtgccgacgt tgatcctgca cggtgacgac gaccagatcg
     2461 tgccgatcgg cgccgcggcg atgctatcgt cgaagctcgt aaagggcgcc gtcctgaagg
     2521 tctacaaggg tgccccacat ggaatgtgca cgaccctaaa ggaccaggtc aacgaggagt
     2581 tgctcgggtt ctttaaagcg tagtcggttt agcgtcgaaa gccatcccga ccgtatcgat
     2641 gccgatcaca aatagcggat caataaaatt tcaaaggagg atagaacaat ggtcactaaa
     2701 actgcaacgc caacggatct cagcgggaat gccgttgaag aaatatcgag caacttgcgt
     2761 cgactgcttg ccgacgtctt cgcgctttac gtgaagacga agaacttcca ctggcacatg
     2821 agcgggcagc actttcgcga ttaccacctg ttgctcgacg agcaggccac acaaatcttc
     2881 gccatgaccg acgacatcgc ggaacgcgcg cgcaagatcg gcgggacaac gatccgttcg
     2941 atcagcgata tcgccaaaaa ccagcggctg aaagacaaca ataaagaagg ggtgaccccg
     3001 caggagat
//
LOCUS       DASSWZ010000290         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1355948, whole genome shotgun sequence.
ACCESSION   DASSWZ010000290 DASSWZ010000000
VERSION     DASSWZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355948"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VFN58_07045"
     CDS             <1..424
                     /locus_tag="VFN58_07045"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917782.1"
                     /translation="MPYCIAAAVVDHQVTLDTFTSHKMEDRSIVEARKKVHLSFPDVP
                     IWPGLADVGPDTEFVGNPLTIRTTDGRSYRARVDIPRGDPALPLTDDELLSKYRDCGR
                     SQLRPNDIERSVNLVLGLETVTNIGTLMSSFSSPSREP"
     gene            complement(639..983)
                     /locus_tag="VFN58_07050"
     CDS             complement(639..983)
                     /locus_tag="VFN58_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HET8917783.1"
                     /translation="MALVRCVTEMGMGVDVHGLDYTNAAKRAVFDAIHHSSLGFPRLV
                     GKTADDMRVDVTIGVPKPDAVDRQAVLETLPHGGGHINVVQGGLQVLNEKGDDGWLIA
                     NAIIVVSIDDGK"
     gene            complement(1186..>2811)
                     /locus_tag="VFN58_07055"
     CDS             complement(1186..>2811)
                     /locus_tag="VFN58_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET8917784.1"
                     /translation="ASLLWNSFKPVRLGKMADFTLQNFTLQNYSNALIDPETISMVGN
                     SFVFATGSTLVALVLGGVVAFLVERTNIAFKKIAHVLMLIPLITPGVLKASAWVLLLN
                     PNNGFLNQLWFSLGFKSYLFNDKSVLSMVWVQGISMIPVTFLLLGAAFKMMDPALEEA
                     SYAAGAGKIRTLRRITIRLMVPAIAAAGLLNFTRALESFDIPLIMGLGAGYQMFSTAI
                     YYALRILSPPAYGLALAYSVVLVAFATFGLLIYQRILNRGYRYRTITGKAFRPTLIDL
                     AHWKYALGIVVLSFSFISFLLPVLVLLWASLLPYYQLPSAEALSLLTLENYIALPRKR
                     IFMSALANSLKLGIVVSVGGMILAVLISWTVLKLRPRGSQILDFLSFVSYGIPGVVAG
                     FGFMIIFLSFPNPLYGTIWLVGLAYCINLLPVATRFSHTGIIQMHQELEEAASTCGAG
                     FVTTLRTITMPIIFPYLAAGGLFLFLLTMRLLSLVAVLYTADSIVFPVLIVQLHDTGF
                     IPQLSALGIMMIVVLSLLTLAVRKLVQDKVISG"
BASE COUNT          761 a          746 c          722 g          582 t
ORIGIN      
        1 catgccctac tgcattgctg ctgcggtggt tgaccaccag gtgaccctcg acacttttac
       61 ctctcacaag atggaagacc gcagtatcgt cgaggccagg aagaaagttc acctttcctt
      121 ccccgatgtg cccatttggc ctggcctggc ggacgtcggc ccggataccg agttcgtcgg
      181 taatccgttg acgatccgga cgacggatgg acgaagctac cgcgcacggg tagacatacc
      241 tcggggtgac cccgctctac cgctgaccga cgacgagctg ttatcgaagt accgggactg
      301 tgggcgcagc cagctgcggc cgaacgacat agagcgcagc gtaaacctgg tgctggggct
      361 agagacggtg acaaatatcg ggacgctgat gtcgagtttc agctcaccgt cccgcgagcc
      421 ttaagatctg atagaacgcg accttcgacg ccgactctga gtttcagcga aattgtagaa
      481 aaagcgcgac gagccaaccg agcttacggc gatttatctg aaagtttaga tgcaaagtct
      541 cgggccgtca ccgatccgtt gaacggagcg aagcgattga acccttcgat agactcaggg
      601 cgggcttttg caacggttgg aaccctcact gacacgaact atttgccgtc gtcgatgctg
      661 acgacgatga tggcgttggc gatcagccaa ccatcgtcgc ccttttcatt tagcacctgc
      721 aatccgcctt gaaccacgtt gatgtgcccg ccgccgtggg gcaacgtttc cagcacggcc
      781 tggcgatcca ccgcgtcggg cttgggcaca ccgatggtta catccacccg catatcgtcg
      841 gccgtcttgc ccaccaaccg cggaaatccc aagctgctgt ggtgaatcgc atcgaacacc
      901 gcccgtttgg cggcattggt gtagtccaat ccgtgaacat ccactcccat tcccatttct
      961 gtaacgcacc gtaccaaggc catggctggt cacctccacc tgcgagtttt agtcgaaccg
     1021 attcttccga tactcattag caacggtagc actcctccgt caagctttcc gccggcttcg
     1081 gcccgagaag cttttgacct tctcggcgaa agccaaactg ccaaccacaa tggtttccaa
     1141 ccaccttcgc tctttaagag ttccgaagct cgcggaatcc acacgctatc ccgagatcac
     1201 cttatcctgc acgagctttc gcaccgctaa ggttagaagg gaaagcacta caatcatcat
     1261 aatccctaag gccgaaagtt gcgggataaa gcccgtatcg tggagttgga caatgagaac
     1321 gggaaagaca atggagtcgg cagtgtaaag taccgctacg agcgaaagaa gtcgcatggt
     1381 taataaaaac aagaataatc cgcccgcagc aagataagga aaaatgatgg gcatggtaat
     1441 cgttcgaagc gtggtcacga atcctgcccc acacgtggag gccgcctctt ccagctcctg
     1501 atgcatttga atgatgccgg tatgactgaa tcgagtggcg acgggcagga ggttgataca
     1561 ataagccaac cccaccagcc agatcgttcc atataatgga ttagggaaag ataggaatat
     1621 aatcatgaag ccgaatccgg caacgactcc agggatcccg taggagacaa aggataagaa
     1681 atccaaaatc tggctccctc tgggacgtag cttaagcacc gtccacgaga tcagcaccgc
     1741 taagatcatg ccgccgacac tgacgacgat gcctaacttc agagagttcg cgagagcaga
     1801 catgaaaatt ctttttcttg gaagggctat atagttttct aacgtcaaca atgacaacgc
     1861 ctcggctgaa gggagctgat agtacgggag caatgaagcc caaaggagaa ctaacacggg
     1921 aagaagaaag gagataaaac taaaagacag aaccacgatg cctagagcat acttccagtg
     1981 tgccaggtcg atgagcgtag gtctaaaagc ttttccagtg atagtcctat acctatagcc
     2041 ccgatttaga atccgctgat aaattagcaa gccaaacgtc gcaaaggcca cgagcacgac
     2101 actatatgcg agagcgagtc cataggcagg gggggacaga attctgaggg catagtaaat
     2161 cgcggtactg aacatttgat atcccgcgcc aaggcccata ataagcggaa tgtcaaaaga
     2221 ctctaaagcg cgagtgaaat tgagaaggcc ggcagcggcg atcgcaggga ccattagcct
     2281 gatcgtaatc ctgcgcagcg ttcgtatttt cccagcacct gctgcatagg acgcttcttc
     2341 gagggcaggg tccatcattt taaaagcggc accgagcagc aggaatgtta cgggtatcat
     2401 ggagataccc tggacccaga ccatcgaaag cacagattta tcgttgaaca gataactttt
     2461 aaatccgagg gagaaccaca attgattcag gaatccgtta ttggggttca aaagaagaac
     2521 ccaagcacta gccttcagca cccccggcgt gattaagggg atcagcatta gcacatgagc
     2581 gattttctta aaggcaatat ttgttcgctc cacgagaaat gcgacgaccc cccccagaac
     2641 taacgcgacc aacgtcgagc ctgttgcaaa cacaaagctg ttgccgacca tagagatagt
     2701 ttcggggtca ataagagcat tgctataatt ttgtagagta aagttttgca atgtaaaatc
     2761 agccattttc ccgagcctta cgggtttgaa gctgttccaa agtaatgaag c
//
LOCUS       DASSWZ010000291         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_676421, whole genome shotgun sequence.
ACCESSION   DASSWZ010000291 DASSWZ010000000
VERSION     DASSWZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676421"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="holB"
                     /locus_tag="VFN58_07060"
     CDS             complement(<1..777)
                     /gene="holB"
                     /locus_tag="VFN58_07060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET8917785.1"
                     /translation="MGFSQIVGHQKQVEIIRQALNHGRLHHAYLFVGAEGVGKKTIAI
                     GLAKAIHCSAATGDFCGACPDCARIQDTNHPDVRIIEPLAGKKEISIQQIRELEKELN
                     FRSFSGKKKIVILDPATLMNLSAQNALLKTLEEPPRDSLLILIAANGGALLPTLRSRC
                     LRISFGPLARDLVSGFLVSRKGLEADTAEFLAAMSLGSLGAVVSIDTQELLERRREWV
                     RLISDLGAGDYRAATDAAEILAGSKEDSLRFLEWVESWFRD"
     gene            complement(778..1416)
                     /gene="tmk"
                     /locus_tag="VFN58_07065"
     CDS             complement(778..1416)
                     /gene="tmk"
                     /locus_tag="VFN58_07065"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET8917786.1"
                     /translation="MIRFITFEGGDGSGKTTQLKALESYLTARGKSCLSTREPGGTSL
                     GELIRQVLLEVSKQPITSPTELFLYLADRAQHIHEVIIPALEQGKIVLCDRHTDSTLA
                     YQGYGRGIDLGLLQSLNDIASQGIKPDLTLLFDCPAEIGLSRTARRQSHTASGRNEDR
                     FEREKIEFHERVRAGFLELARAEPNRFRIIDAARSAKEVGQEIKKIIDRELT"
     gene            complement(1510..1893)
                     /locus_tag="VFN58_07070"
     CDS             complement(1510..1893)
                     /locus_tag="VFN58_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8917787.1"
                     /translation="MDSKKTIMVVDDNPDIITIIKTILEGKGYNVISASSGQELLNLL
                     KNQKPDLIILDIMMPEMDGLEVLTKLKGVTDTTSIPVILLTAKVQYEDVLGGYKLGAD
                     YYITKPFTSTQLINGINLLLGERKS"
     gene            complement(2007..>2221)
                     /locus_tag="VFN58_07075"
     CDS             complement(2007..>2221)
                     /locus_tag="VFN58_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917788.1"
                     /translation="LGARPNSWNAAITDAGKCLSITSLNSYSRLLAQRESLIKNQSSA
                     AVFLMFTSAPEDVKLSTHKAFLSRRC"
BASE COUNT          535 a          603 c          535 g          548 t
ORIGIN      
        1 atcgcgaaac caactttcaa cccattcgag aaacctcaag gaatcttctt tgcttccggc
       61 aagaatctct gcggcatcgg tggccgcacg gtaatctccc gcacctaaat cggaaatcag
      121 ccgcacccat tctcgccgcc tctcgagcaa ctcttgcgtg tcaatgctaa cgaccgcacc
      181 taagcttcct aagctcatcg ctgccaagaa ctccgccgtg tccgcctcta atcctttgcg
      241 ggagaccaaa aaaccgctga cgaggtctcg tgccagtgga ccgaaactaa tgcggaggca
      301 gcgcgatcgt aaggtcggca agagtgcccc gccgttggcc gcaattaata tcaacagaga
      361 atctcgaggc ggctcctcaa gggttttcaa gagcgcgttt tgtgcggata gattcatcaa
      421 agtggcagga tccaagatga cgattttttt cttgccggaa aaggatcgaa aattcagttc
      481 cttttccaac tcacggattt gctgaatgct gatctctttt ttaccggcca gaggctcgat
      541 gatgcggaca tccggatggt tggtgtcttg aatgcgcgcg caatccgggc acgcgccaca
      601 aaaatcgccg gtcgccgccg agcaatgaat tgctttcgcg aggccgatag caatggtttt
      661 cttacccaca ccctccgcac ctacgaaaag ataggcatga tgcagccgcc catggttcaa
      721 cgcttggcgt atgatttcta cctgtttttg atggccgacg atttgcgaaa aacccatcta
      781 tgtaagctct ctatcgataa tcttcttgat ctcttgcccg acctctttag cggatcgcgc
      841 cgcatcgatt atgcggaatc tgtttggctc ggcccgcgct aactcaagaa accctgcacg
      901 aaccctttcg tgaaactcga tcttttcccg ctcgaaccga tcttcgtttc gtcctgaagc
      961 cgtgtgggac tgacgccgag cagttctaga caacccgatt tccgccgggc agtcaaacag
     1021 cagggtgaga tccggcttga ttccctggct agcgatatca ttgagacttt gtaacaaccc
     1081 gaggtctatc ccgcggccat aaccctgata ggcaagtgtc gagtccgtgt gccggtcgca
     1141 aagaacgatc ttgccctgtt caagcgcagg aatgatgacc tcgtggatat gctgcgcccg
     1201 gtccgccaag taaaggaata attccgttgg cgacgtgatt ggttgcttgc tgacttcgag
     1261 tagaacttgc cggataagct cccctaacga agtcccgcca ggctcccgag tcgatagaca
     1321 ggatttaccg cgcgccgtca gataactctc gagggctttg agttgagtgg ttttcccgct
     1381 cccgtcacca ccctcgaaag tgatgaaacg aatcattaga aaaataactc acgccggttc
     1441 ggcgtaacag aaactcacag cgaaacggca cacgcgtgga cggaaatgca aaccagggat
     1501 acgggagagc tagcttttcc gctcccccaa aagcagattg attccattga tcaattgagt
     1561 gctcgtgaat ggcttcgtga tgtaataatc agctccgagc ttgtatcctc ctaagacatc
     1621 ctcgtattga accttggcgg tcaatagaat cactggaatg ctggtcgtat cggtaactcc
     1681 tttgagtttg gtcaaaacct caagaccatc catttcaggc atcataatgt ctaaaataat
     1741 caggtccggc ttttgattct tcaaaagatt taacagctct tggccactag aagcgctgat
     1801 tacgttatac cctttaccct ctaagatagt cttaatgatc gtaataatat cgggattgtc
     1861 gtcgaccacc atgatggtct tcttgctatc catgcttccc cctctagcgc cgccgttttc
     1921 gtttacgccg ttaggctatc gggcttcttt gcgtcagcca atccacacgg aggccttaac
     1981 gggaatccat cccgtggccg aattcttcaa caacgacgtg acaaaaaagc cttatgagtt
     2041 gagagcttaa catcttccgg cgctgatgta aacatgagaa aaacagcagc cgatgactgg
     2101 tttttgatca gggattcacg ctgcgccagt aatcttgagt aactgttgag ggaagtgatg
     2161 gataggcact ttccagcatc ggtaatggct gcattccagc tgtttggacg agcgccgagc
     2221 g
//
LOCUS       DASSWZ010000292         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_436095, whole genome shotgun sequence.
ACCESSION   DASSWZ010000292 DASSWZ010000000
VERSION     DASSWZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436095"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFN58_07080"
     CDS             complement(<1..846)
                     /locus_tag="VFN58_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET8917789.1"
                     /translation="MGLLSSEELKQLLPAETSAFPSPIPTQSVSSDEFMPAPQTAKQR
                     EFEARIKEYGKELARHQGMSRRAFFKSAAGMAAAFVAMNDTYGPLYKASRAEAAIPEM
                     AGERAQGLSKQFIMDMHTHFLRDDTRIMNFVRQREAVGKAGWNPALQGKPQTIEDLKF
                     ANYFKEVFLDSDTQVACISGSPSEEPLDWFLTNEMKNEARAKINQQAGTKRAFAHAIF
                     TPGWPGWLDKVDEAIEKLKPDSFKGYTIGDNTNKHLAKHPYRLDNEKLMYPFYEKLVK
                     AGLVNV"
     gene            complement(1040..2035)
                     /locus_tag="VFN58_07085"
     CDS             complement(1040..2035)
                     /locus_tag="VFN58_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917790.1"
                     /translation="MRRAWIAVAAILFLCHAKGSVAAAEDLRAASGGFTTAIHAVLWA
                     AYEEKIFRKYGLDVEYLALNSGRLGMQMLLSDDVQILFSTGVTVVTTNLQKADLAIIG
                     GGLNFFPTKLIVRPEIKKPSDLRGKVLAVGGFGAANHMALLVALEKLGVNAKEVTIIQ
                     VAGGAAQLSALTKGVISGLVFNEPQATIAVKKFGMQSLIDLMESKTPFPQNCFIVKRS
                     YLDSNRDKVTNFMKAVVESIYLVKKDRPLAIRLIKKYIRVNDEEAAIGYDYYLAKHSE
                     GILDLPDRRGLVFVIEETAKTNPKAAGQTPETLRLLEPSVLDEIKKSGFAEKVKQ"
     gene            complement(2248..2709)
                     /gene="trmL"
                     /locus_tag="VFN58_07090"
     CDS             complement(2248..2709)
                     /gene="trmL"
                     /locus_tag="VFN58_07090"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HET8917791.1"
                     /translation="MHVVLFQPEIPPNTGSIARLCAATLTPLHLIEPLGFKIDDKHLK
                     RAGLDYWEFVDLRVYKSWDDFLSQSITQNLLYFSKRASRSFTRACYQENDYLIFGPET
                     RGLPQKLLDANPERSYRIPMMGTGVRSLNLSNAVSIVLYEGLRQLGKLDQI"
     gene            complement(2715..3152)
                     /gene="dtd"
                     /locus_tag="VFN58_07095"
     CDS             complement(2715..3152)
                     /gene="dtd"
                     /locus_tag="VFN58_07095"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET8917792.1"
                     /translation="MRAVIQRVSRAEVRVAGEPVGRIGPGLCVFLGIGKADGQANVDQ
                     LADKIKNLRIFEDENGKMNLSVEEVREEVLVVSQFTLYGDCRKGNRPSFSNAAPPDQA
                     EKLYEYFVRRLRGAGLTVATGQFKARMDVTLINDGPVTFIVES"
BASE COUNT          701 a          883 c          859 g          772 t
ORIGIN      
        1 gacgttgacc aggcccgctt tgacgagctt ctcgtaaaac gggtacatca gcttctcgtt
       61 atccaggcgg tagggatgct tggccagatg cttgttggtg ttatcgccga tggtatagcc
      121 cttgaatgag tccggcttca atttctcgat ggcctcgtct accttatcca gccagccggg
      181 ccatcctggc gtaaaaatcg cgtgcgcgaa ggcgcgcttc gttccggctt gctggttgat
      241 cttcgcgcgc gcctcattct tcatctcgtt agtgaggaac caatcgagcg gctcttcgga
      301 cggcgagccg ctaatacagg cgacttgggt gtcgctgtcg aggaacactt ccttgaagta
      361 gttggcgaac ttgagatcct ctatggtttg aggcttaccc tgcagcgccg ggttccaacc
      421 ggctttgccc accgcttcgc gctggcgcac gaagttcata atacgcgtgt cgtcacgcag
      481 gaagtgggta tgcatatcca taataaactg cttggatagc ccctgggcgc gctcgcccgc
      541 catttcgggg atagccgcct cggcccggct cgccttatac aatggaccgt aagtgtcgtt
      601 catggcgaca aaggccgcag ccatgcccgc ggcgctcttg aaaaaggccc gccggctcat
      661 gccctgatga cgcgccagct ccttgccgta ctccttgatt cgcgcctcga actcacgttg
      721 cttcgcggtc tgcggtgccg gcatgaactc atcgctggac accgactggg tcgggatcgg
      781 cgaaggaaaa gctgacgtct ccgcaggcaa caattgcttc agttcttcag agctcaacaa
      841 acccataacg acctccttgg tttagttaga atgaccggaa atcacgtcca atcttctggg
      901 gctagccggg agatccagga ctgacagcga agtgaccaga tccatagacc cagccgcaag
      961 atgtgaacgt aaaataggtc taaaaagttt gaacgtcaag gcgaatttgc gacgcccggt
     1021 ggggctgacc ggcccctcct tactgtttta ctttttccgc aaagccgctt ttcttgatct
     1081 catcaagcac ggatggttcc aggagcctga gtgtttcggg agtctgaccc gctgctttgg
     1141 gattggtttt cgctgtttcc tcaatgacga acaccagccc gcgacggtcg ggaaggtcca
     1201 agatcccttc gctgtgcttg gccaaatagt aatcgtagcc gatggccgcc tcttcgtcgt
     1261 tgacgcggat atattttttt atcagccgga tcgccaatgg acggtctttt ttaacaaggt
     1321 aaatcgattc aacaaccgcc ttcatgaaat tcgtcacctt atcccggttg gaatcgaggt
     1381 agcttcgttt gacgatgaaa cagttctggg ggaaaggggt cttgctttcc atgaggtcga
     1441 tcagggattg catgccgaac ttctttaccg cgatagtcgc ttgcggttca ttgaacacca
     1501 agccagagat aacacccttg gtgagcgcgc tgagttgcgc cgcgcctccc gccacttgaa
     1561 tgatagtgac ttctttggca ttgacgccca acttttccag cgccacgagc agcgccatgt
     1621 gattggccgc gccgaagccg cccacggcca gaaccttccc cctgagatcc gagggttttt
     1681 tgatttcggg gcgaacgatg agcttagtag ggaaaaaatt caacccgccg cctatgatgg
     1741 ccaggtcggc cttttgcaag ttagtggtta caaccgtaac cccggtggag aaaagaatct
     1801 ggacatcatc ggagaggagc atttgcatgc ccaatctccc gctgttgagg gctagatatt
     1861 caacatcgag accgtatttg cggaaaatct tctcctcata tgcggcccac agcaccgcat
     1921 ggatcgccgt ggtaaatccg ccgctggctg ctcgaaggtc ttccgccgcg gcgacagatc
     1981 cctttgcatg acaaagaaag agaatagccg caactgcgat ccacgctctt ctcatcggcc
     2041 cagccttttc ctgctaggtg agccagtcca agttatcgag cccaactgta ttggagaaag
     2101 ttggtacgac gcagattcca tgggcagacc caccttattt gagcgcaggc ttagcatcga
     2161 gtagcagcaa ccgtcaacgc gcagcattgg ctcccagtga agattggcca cgcgggttgt
     2221 ttgaatgtgt tccaagaaaa atagcgacta aatttgatca agttttccca actgccggag
     2281 tccttcgtaa agcacgatcg aaacggcatt ggataagttc aagctgcgca ccccggtgcc
     2341 catcatggga atgcggtaag agcgctcggg attggcgtcc aggagtttct gtggcaaccc
     2401 gcgggtctcc gggccgaaga tcagatagtc gttttcttgg tagcaagccc gagtgaaaga
     2461 tcggcttgct cttttggaaa agtagagaag attttgagtg atactctggc ttaagaaatc
     2521 gtcccacgat ttgtacactc gaaggtcaac gaactcccag tagtcgagcc cagcacgttt
     2581 tagatgcttg tcatcgatct taaatcccag tggctcgatc aagtggagcg gtgtcagggt
     2641 tgccgcgcac agccgagcaa tgcttccggt attgggcggg atctccggtt gaaacaagac
     2701 gacgtgcatt ttccctagct ctccacgatg aaagtgaccg gtccgtcgtt gataagagtg
     2761 acatccatcc gcgctttgaa ttgtccggtc gcgacggtaa ggccggcgcc gcgcaatcgc
     2821 cgcacgaaat attcgtataa tttctcggct tgatcgggcg gcgccgcatt gctaaaggaa
     2881 ggccgattgc ccttccggca atcgccatac agtgtgaatt gcgatactac caaaacttct
     2941 tcccgtactt cctccacgga aagattcatc ttgccattct cgtcctcaaa aattctcaga
     3001 tttttgatct tgtccgcaag ctgatcgacg ttagcttggc catcggcttt accaattccc
     3061 agaaaaacac agagcccagg gccgattctg ccgaccggtt cgccggcgac cctaacctcg
     3121 gctcgggaaa ctctttggat aacggctctc acggcgaact agtgctggag atggtgggtt
     3181 ttttcagctt gcgcatcaac tggatcttgc aggga
//
LOCUS       DASSWZ010000293         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1129284, whole genome shotgun sequence.
ACCESSION   DASSWZ010000293 DASSWZ010000000
VERSION     DASSWZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129284"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFN58_07100"
     CDS             <1..981
                     /locus_tag="VFN58_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8917793.1"
                     /translation="MVFTKDPRTGTRNVGLYRMQVFDRRTTGMHWHLHKVGARHFLHQ
                     KERAGRLELAACLGGDPVMIYAATAPLPDQIDEILFSGFLRKKGVELVKGITVDVEVP
                     ANSDIVIEGYVDPAEPLRREGPFGDHTGFYSLADDYPVFHVTCITHRKNPIYPTTIVG
                     RPPMEDAYLGKTTERLFLPLVRVTLPEIVDMHLPVHGVFHNLAIISIKKEYPGHARKV
                     MHALWGLGQMMFTKILLIVDHDVNVQDLSEVTWVVGNHIDPKRDAIFVEGPVDVLDHA
                     APVMGFGSKMGIDATRKWRSEGFEREWPDAIVMDDKIKQYIDSIWEKLGI"
     gene            978..1931
                     /locus_tag="VFN58_07105"
     CDS             978..1931
                     /locus_tag="VFN58_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HET8917794.1"
                     /translation="MNFRFSMSDSRTSPRSKIPNLKSKIADYGRLIRFSHTVFALPFA
                     LASVALAWPSHPVTLRSFFWILIAMVGARSAAMAFNRLADRKFDALNPRTQQWELPHG
                     TVKVWEAVILTITSSLVFVYAAYQLNFVCFVLSPVALTIVFFYSLTKRFTWASHLFLG
                     LALSLAPMGAWLAVSGSPIDLPELATPFFLGLAVLFWLAGFDIIYSLQDREFDQKEGL
                     YSIPVRFGVASALRLSSFFHACTVVFFAAVGLSAQLGVIYWSGFVIVAIILFWEHRLV
                     TPTDLSRINKAFFDLNAYVSIGYLLTTVADLFFITRSGIGQ"
     gene            1928..2719
                     /locus_tag="VFN58_07110"
     CDS             1928..2719
                     /locus_tag="VFN58_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET8917795.1"
                     /translation="MSPALIVSLASVGTIGGFFAGVLGFGGGVVMFPLLYYVPPLLGL
                     ARLDAKTVAAVVITQVFFSSLVGGLAHLRSGRVHGRIALVAGMTSAVGSFIGGVASKW
                     TPERFLLLLFGIATLLVIAMMFLPAPSEELDKVSIEKVTVAPAPLSLCSFVTGILIGF
                     LGAGNFVFVPLLIYVLKVPTRIAIGSSLFIAMMNTCSGFLGKLATGQIPWLIATAVVI
                     GAGIGALVGEKIHRRLPTPILRYVYSAMVGLIAARIWLSILDFDL"
     gene            2814..>3328
                     /locus_tag="VFN58_07115"
     CDS             2814..>3328
                     /locus_tag="VFN58_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917796.1"
                     /translation="MKTKNSLALFSLFVFFAGCTSLQTGGEVQWGRQALLEGKSEAAL
                     GYFYSAAQRDPNYVYATGSSPRQGVWSYVGRSEYLTGRFPQARQSLERAVAANQQEDI
                     ARLYLGLTLAREGDRQRGLKEIEGGMRGINNFLDYINQAQRFSIGQFWDPDRDIRRAI
                     QSDLAMISGKDL"
BASE COUNT          652 a          945 c          885 g          846 t
ORIGIN      
        1 atggtcttta cgaaggatcc ccgcaccggt acgcgcaacg tcggccttta tcgcatgcag
       61 gtcttcgacc gccgcacgac ggggatgcac tggcacctcc acaaagtcgg tgcgcgccat
      121 tttctccacc aaaaggagcg cgccggaagg ctggaattgg cggcctgctt gggtggtgac
      181 ccggtgatga tctatgccgc caccgcgccg ttgccggatc agatcgacga aatcttgttt
      241 agcggttttt tgcgcaaaaa aggagttgaa ctggtcaaag gcattaccgt agatgtcgaa
      301 gtgcccgcca actcagatat cgttatcgaa ggctacgtgg atcctgccga gcctttgcgc
      361 cgcgagggtc cgttcggcga tcacacgggc ttttactcac tggcggatga ctacccggtc
      421 tttcacgtta cctgcatcac gcaccgaaaa aaccccatct acccgaccac catcgtgggc
      481 cggccgccga tggaggacgc ctatctaggc aagacgacgg agcggctctt cttacccctg
      541 gtgcgcgtga ctttgcccga aatcgtcgac atgcacctgc cggtgcacgg cgtctttcat
      601 aatctggcga ttatttcaat caaaaaagag tatccggggc acgcgcggaa ggtaatgcac
      661 gctctgtggg gcctgggcca gatgatgttt accaaaattc tgctgattgt ggatcatgac
      721 gtcaatgtgc aggatctttc cgaagtcacc tgggtcgtgg gcaaccatat cgatcccaaa
      781 cgggatgcca tctttgtcga agggcccgtg gatgtcctgg accatgccgc ccccgtcatg
      841 ggctttggct ccaagatggg catcgatgcg accagaaagt ggcgcagtga gggcttcgag
      901 cgtgaatggc ccgatgccat cgtgatggac gacaaaatca agcagtacat cgactcgatc
      961 tgggagaaac tcgggatatg aattttcgat tttcaatgtc cgattctcga acctcccccc
     1021 ggtccaaaat cccaaatcta aaatccaaaa ttgccgatta cggccgcctc attcgtttct
     1081 cccacacagt cttcgcgctg cctttcgcgc tcgcatccgt cgccctcgcc tggccatctc
     1141 atccggtcac tcttcgcagc tttttctgga ttctgatcgc catggtcggg gcgagaagcg
     1201 cggccatggc cttcaatcgc ctggccgatc gaaaatttga cgccctcaat ccacgcacgc
     1261 aacaatggga actcccccac ggaaccgtca aggtatggga ggctgtaata ctgaccatca
     1321 ccagctccct ggttttcgtt tacgccgcgt atcaactcaa cttcgtctgc tttgttctgt
     1381 cccccgtcgc cctcacgatt gttttctttt attccctgac caagcgtttt acctgggcgt
     1441 cccatttatt tcttgggctt gcgctttcgc tcgctcctat gggcgcgtgg ctagcagttt
     1501 cgggctcgcc catcgattta ccagagctgg caacgccttt tttcttaggc ctggcagtac
     1561 tcttttggct agcgggcttc gacatcattt actcgctgca ggaccgtgaa ttcgaccaaa
     1621 aagaggggct ttattccatt ccggttcgtt ttggcgttgc atccgctttg cggctttcca
     1681 gtttcttcca cgcctgtaca gtagtttttt tcgcggcggt agggctttct gctcagttgg
     1741 gagtcattta ctggtccggc tttgtgatcg ttgccatcat tttgttctgg gagcaccgtc
     1801 tggttactcc caccgatctt tcgcggatca ataaggcttt ctttgaccta aatgcttacg
     1861 tgagcatcgg ctacttgctg acgaccgtag ccgatctttt cttcatcacc cgctccggga
     1921 tcggacaatg agccccgcgc tcatcgtcag cctggcctct gttggcacca tcggcgggtt
     1981 ttttgccggc gtgttgggtt tcggtggagg agtggtgatg tttcccctcc tctactacgt
     2041 gccgccgctg ttaggcttgg caaggctcga cgccaagacc gttgcagcgg tggtcatcac
     2101 ccaggtattt ttttcgagcc tagtcggcgg attggcccac cttcgtagcg gccgcgtcca
     2161 tgggcgaatc gcgcttgtcg ccgggatgac atcggccgtc ggctctttta tcggcggagt
     2221 ggcgtcaaaa tggacacctg aacgatttct actgttgctt ttcggcatcg ctacccttct
     2281 ggtaattgcc atgatgtttc ttccggctcc gagcgaggag ctggataagg tttcgataga
     2341 aaaggtgacg gtagcgccgg ctcctttgtc tctatgctcg tttgtcacag gcattctcat
     2401 cggctttctg ggcgcgggaa atttcgtctt tgttccgttg ctgatctatg tcctcaaagt
     2461 cccgacacga attgccatcg gcagcagcct gtttatcgcg atgatgaata cctgctcggg
     2521 atttctcggc aagcttgcca ccggccaaat accttggttg atagcaacgg ctgtggttat
     2581 cggagcgggc atcggagcgc tggttggaga gaaaatccac cgtcggcttc ccactcccat
     2641 tcttcgctat gtttacagcg ccatggtcgg acttatcgcc gcccgaattt ggctcagcat
     2701 actcgacttc gacctttaaa aagcgctctc cgagcttcct aaacgcgacc gtttcagtat
     2761 tgcttttccc aattgcactg actatgctga gtccagaaaa acaggaggac gctatgaaaa
     2821 caaagaactc attggcattg ttctcattgt ttgttttctt cgcggggtgc acgagtcttc
     2881 agaccggcgg cgaagtacag tggggacggc aggctctact cgagggcaag agtgaagccg
     2941 ccctcggcta tttttacagc gccgctcaga gggatcccaa ctacgtctac gccaccggat
     3001 catcaccgcg gcaaggcgtt tggagttatg tcggacgatc agagtacctt accggcagat
     3061 ttccccaagc ccggcaaagc ttggagcggg cagttgccgc caatcagcaa gaagatatcg
     3121 ccaggctcta tttgggtctg accttggccc gcgaaggcga tcgccagcgc ggcttaaaag
     3181 aaatcgaagg cgggatgcgc ggcattaaca attttctcga ttatatcaat caagcccagc
     3241 gattttccat tggacagttc tgggacccgg acagggacat acgccgggcg attcaaagtg
     3301 acctggcgat gatctccggt aaagatct
//
LOCUS       DASSWZ010000294         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_323043, whole genome shotgun sequence.
ACCESSION   DASSWZ010000294 DASSWZ010000000
VERSION     DASSWZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323043"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VFN58_07120"
     CDS             complement(<1..1802)
                     /locus_tag="VFN58_07120"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET8917797.1"
                     /translation="MPPEVFNLANYSFNPYAIPTLIAAVAIMALGFIVLVGERNSRVS
                     LSFFIMTVTAAIWLFCYSLMYCAVAAPVASAWAVMGQFGITFIPAAVYHFTVGTLAIY
                     SKHKSRVWLAWLVSGFFFVTALYSDAFISGVNHFWWGYYARYEWASIPFLVFFFGLMA
                     LSLQHYWQEYHAPAPGAEKLRSKALFVAFCIAYLGSFDYLAAFGIPFYPFGHLAVLGF
                     IILTARAVWRFRLVDITPAFAATEIINAMADALLVLDNEGTVRVANRAACQLFRRPES
                     ALIGSGIQNLTSIIAPPEFILDRVILSGSIRDYEFPLIDEHAGRATLSVSSFVMRDDP
                     AMDPLALVCIVRDVTEQRQAQLDIHRHTERQVALYEINLATTSTLELRVVLNVLLEKL
                     TTLVPETATTIMLLEKKAGQLVKVAARGIDEEAWKAHSTGHGEGTYPALPAKGAIRIS
                     NIQVSRNGLDLDYFRSSGFVSYLGVPLIAQDKVLGILSFYCRQERYFSDEEINFLRTL
                     AGQAAGAIQNSQLYEQTKQQAMDLDKANKVKDEFLGVMSHELRTPRNVIAGYTKIVQD
                     GVLGEINAEQVDALDKVTRHSNELLVMVNSIMDAT"
     gene            complement(1823..2593)
                     /locus_tag="VFN58_07125"
     CDS             complement(1823..2593)
                     /locus_tag="VFN58_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET8917798.1"
                     /translation="MSPASTSRAPLIGITADLASVPTKGTNPTQESTLFLPQRYCRAI
                     QEVGGIPLILPPIASQSGLRRILQRLDGVLISGGNFDIHPSYYGEKPIRALGAIKKDR
                     TEFELELVDLSLNEDLPLLGICGGAQAINVALGGSLYQDIATQLPNAAKHEQGTKRDK
                     GGHPILIHSGTRLRQIIQRQTLKVNTTHHQAVRRVGKGLVVNATAEDGLIEGLESSNH
                     RFVLGVQWHPEILARKVPSQRRIFSSFISASRLLHRGI"
     gene            complement(2590..>2986)
                     /locus_tag="VFN58_07130"
     CDS             complement(2590..>2986)
                     /locus_tag="VFN58_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET8917799.1"
                     /translation="LIRQESLFDPQARSPAAALGLMQLLPSTASRVAKQIGISAPSSE
                     QLFQPEINLVLGTQYLKDLLQRYSNDWFKAIAAYNAGEAAVDRWEKEILTDDAEEFVE
                     RIPYLETRGYVKLVMRNHRIYKRLYDQQK"
BASE COUNT          685 a          759 c          861 g          681 t
ORIGIN      
        1 gtcgcgtcca tgatgctgtt gaccattacc aacagttcat tcgagtgacg tgtgactttg
       61 tctagcgcat cgacctgttc cgcattaatc tcgcccagga ctccgtcttg gacgattttc
      121 gtatagccgg cgatgacatt ccgcggggta cgtagctcgt gggacatgac ccccaaaaat
      181 tcgtctttga ctttgttggc tttgtcgagg tccatagctt gctgttttgt ctgttcgtag
      241 agctgggagt tttggatggc accggcggcc tggccggcga gagtacgcag gaaattaatt
      301 tcttcatcgc taaagtagcg ctcttgtcgg caataaaaag agagaatccc aagcactttg
      361 tcctgagcta tcaacggcac gccgaggtag gaaacgaacc cgctgctgcg aaaatagtct
      421 aagtctaggc cattgcgaga aacctgaatg ttggaaattc ggatagctcc tttagccggc
      481 agcgcaggat atgtgccctc gccgtggccc gtgctgtggg ctttccatgc ctcttcgtcg
      541 ataccccgcg cagcgacttt aacgagctgc cccgctttct tttcaagcaa catgatcgtc
      601 gtcgcggttt ccggtaccag ggtggtaagt ttttccaaaa ggacgttgag gaccacgcgc
      661 aattccagag tggaggttgt ggcgagattg atttcgtaga gtgcgacctg gcgttccgta
      721 tggcggtgga tatccaactg ggcttgcctc tgctctgtaa catcgcgaac gatgcagaca
      781 agggcaagag gatccatcgc tggatcatcg cgcatgacga aggaggaaac gctcagagtt
      841 gcgcggcccg catgttcatc gatcagcggg aattcgtaat cgcgaataga gccgctcaaa
      901 atgacgcggt caagaatgaa ttctggaggg gcaatgatgg aggtcaagtt ttggatgccg
      961 cttccgatca gcgcgctttc gggacgcctg aataactggc aggcggctcg atttgctact
     1021 cgaaccgttc cttcattgtc cagaacgaga agcgcgtccg ccatggcgtt gatgatctcc
     1081 gttgcggcaa aggccggggt aatgtcgacg agcctaaagc gccacaccgc ccgcgcagtc
     1141 aggatgatga aaccgagcac agccaggtga ccgaacgggt agaaaggtat gccaaaggca
     1201 gctagataat cgaaggagcc gagatacgct atacagaagg caacgaacaa tgctttgctt
     1261 ctcagctttt ccgcgccagg tgccggcgca tgatattcct gccagtagtg ttggagactc
     1321 aatgccatta agccgaagaa gaaaacgaga aagggtatac tcgcccattc gtaacgagca
     1381 taatagcccc accaaaaatg attgacacca ctgataaagg catcgctgta cagggctgtc
     1441 acaaagaaaa atccggagac gagccaagcg agccagactc ggctcttgtg ctttgaatag
     1501 attgctaaag tgccgaccgt gaagtgatag acggccgcgg gtataaaggt gattccgaac
     1561 tgccccatca cagcccaggc gcttgctacg ggcgcagcca ccgcgcaata catgagggag
     1621 taacaaaaga gccagatcgc cgcggtaacg gtcataatga agaaggaaag gctgacacga
     1681 gagttgcgct cgccgacgag cacgatgaag cccagagcca tgatcgcgac cgcggcgatc
     1741 aaggtcggga tcgcgtatgg gttgaaactg tagttagcca ggttgaaaac ctcggggggc
     1801 atgggtctgg aagggtttcg cttcagattc cgcgatgtaa aagtcttgaa gcagaaatga
     1861 aagaggaaaa gatcctgcgc tgggagggca ccttgcgcgc cagaatctcg ggatgccatt
     1921 gcacgcctag tacgaaacga tggttggagc tctcaagtcc ttcaatcagt ccatcttccg
     1981 ccgtggcatt gaccactaac cccttgccga ctcttctgac ggcttggtgg tgggtcgtgt
     2041 tgaccttcaa ggtctgcctt tggatgatct gccggaggcg tgtcccagaa tgaatcagta
     2101 ttggatgacc gcctttgtct ctttttgttc cttgctcgtg tttggcggcg ttgggaagct
     2161 gggttgcgat atcttggtaa agggacccgc ccaaggctac attgatcgcc tgcgcgccgc
     2221 cgcagattcc cagaagcggt aaatcttcgt tcaaagacaa atcaactagc tccagctcaa
     2281 actcggtccg gtccttcttg atcgcaccta aagcacggat cggcttttcg ccgtaataac
     2341 ttgggtggat gtcgaaattg ccgccgctga tcaatacacc gtcgaggcgc tgcagaatcc
     2401 tgcgaagtcc gctctgggaa gcgatcggcg ggaggatgag cggaattccg cccacttctt
     2461 ggattgcgcg gcagtagcgt tggggcaaaa acagggtaga ttcttgcgtg ggattagtac
     2521 ctttagttgg aacactcgcc agatcggcgg ttatgccaat cagcggggcc cggctcgtgc
     2581 tcgctgggct catttttgct ggtcgtatag acgcttgtag attcggtggt tgcgcatcac
     2641 tagtttaacg taaccgcgcg tttctaggta gggtatgcgt tcgacaaatt cctccgcgtc
     2701 atcggtcaga atctctttct cccaacgatc cactgcagct tcgccggcat tgtaggcggc
     2761 gatggctttg aaccaatcat ttgagtaacg ttgcagtaga tcctttagat actgagtgcc
     2821 gagcacgaga ttgatctccg gttggaacag ttgctcgctg gagggcgcag agatcccgat
     2881 ctgcttcgct acccgcgagg cggttgacgg gagcagctgc atcaaaccca gtgccgctgc
     2941 cggcgaacgt gcctggggat cgaataggct ctcttgtcga atcaag
//
LOCUS       DASSWZ010000295         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1058713, whole genome shotgun sequence.
ACCESSION   DASSWZ010000295 DASSWZ010000000
VERSION     DASSWZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058713"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VFN58_07135"
     CDS             <1..1473
                     /locus_tag="VFN58_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET8917800.1"
                     /translation="YGRDLGRGHMVNLGEAIGVIAAQSIGEPGTQLTMRTFHIGGTAS
                     RRAEQTTLESRNDGSVKFINVSTVTNKEGDLVVMNRNGEIAIVDYPEGEKGRERERER
                     YPVVYGAKFKKKDGAKIKAGELLAEWDPYTIPILTEVAGVVKFGDVIEAVTMEEKVDE
                     RTGLSTKVIIDSKDVDKRPRISIKNIEGQTARLSATSEARYLLPVGAHINVQDNQVVD
                     GGDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEFAVISEIDGVVSFGKDTKGKRK
                     VIVTPEVGEPREYLIPKGKHISVHEGDRIRAGEALMDGSSNPHDILNILGEKALAKYL
                     VDEVQEIYRLQGVRINDKHIEVIVRQMLRRVRIKEVGDTDFLVGDHVEKWRFDDENQK
                     AIQQNGKPAVAEPLLLGITKASLSTESFISAASFQETTKVLTEAAIHGKIDLLCGLKE
                     NVIMGRLIPAGTGLARYSKAEIEIEAPEGGDGEIPQAAEA"
     gene            1569..1940
                     /gene="rpsL"
                     /locus_tag="VFN58_07140"
     CDS             1569..1940
                     /gene="rpsL"
                     /locus_tag="VFN58_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET8917801.1"
                     /translation="MPTISQLVRESREKQRRKNTAPALQGCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTL
                     DSIGVQERRQGRSKYGAKRPK"
     gene            1947..2417
                     /gene="rpsG"
                     /locus_tag="VFN58_07145"
     CDS             1947..2417
                     /gene="rpsG"
                     /locus_tag="VFN58_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET8917802.1"
                     /translation="MPRKGEVRKREGLPDPKYGDKLVAKFVNTIMSRGKKSVAESIFY
                     RSLNIVTAKTKEDSLGIFKKAIENARPTVEVRSRRVGGATYQVPIEVRPQRRLSLSMR
                     WLIQSARERGEKSMEEKLAAEFIDAANNRGTAIKKKEDTHRMAEANRAFAHYRW"
     gene            2489..>3773
                     /gene="fusA"
                     /locus_tag="VFN58_07150"
     CDS             2489..>3773
                     /gene="fusA"
                     /locus_tag="VFN58_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET8917803.1"
                     /translation="MARTVPLEKTRNIGIMAHIDAGKTTTTERILYYTGINYKIGEVH
                     EGTATMDWMVQEQERGITITSAATTCLWRDHRVNIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFCSVGGVEPQTETVWRQADKYGVPRIAFVNKMDRLGADFYRVVKMIEDRLGARPV
                     ALQLPIGSEEKFAGIIDLVQMKAVIWEDESLGAKFRVEAIPESMIAEARQYHDKLLEA
                     VADCDESIMEKYLEGKEISESELRRAIRNAALAIKIVPVMCGSAFRNKGVQPLLDAVV
                     DYLPSPLDIPPVKGIDPNSQAAEERPAKDDAPFSALAFKIMTDPFVGTLSFFRVYSGS
                     LTSGASIYNSTKSKRERIGRLLKMHANKREEIKEVYAGDIAAAVGLRTATTGDTLCDE
                     SHPIILESIDFPDPVISIAIEPKSKADQERLGLSLQ"
BASE COUNT          998 a          908 c         1091 g          776 t
ORIGIN      
        1 tatggtcgcg acctgggtcg gggccatatg gtcaacctcg gcgaagcgat cggggtcatt
       61 gccgcgcaat ctatcggtga accgggaaca cagctcacca tgcggacttt ccatattggt
      121 ggaacggcga gccgcagagc ggagcaaacc acgttggaat cgcgaaacga cggctccgtg
      181 aagtttatca atgtgagcac ggtaaccaat aaagaaggcg atcttgtggt gatgaaccgt
      241 aacggtgaaa tcgccatcgt ggattatccg gaaggtgaga aagggcgaga gcgagaaagg
      301 gagcgttacc ccgtcgttta cggtgcgaaa tttaagaaaa aggacggcgc caagatcaaa
      361 gccggggaac tgttggccga gtgggatcct tacaccattc cgattctcac agaagtggct
      421 ggtgtcgtca agttcggcga cgttattgaa gctgtgacga tggaagaaaa agtcgatgag
      481 cgcaccgggc tgtcgacgaa agtgatcatc gactccaagg acgttgataa aaggccgaga
      541 atctccatta aaaatatcga ggggcagacg gctcgtctgt cagcaacctc cgaggcgcgc
      601 tatctcctgc cggtgggcgc ccatatcaac gttcaagata atcaagtagt cgatgggggt
      661 gacgtcctgg caaagattcc gcgagaaacc actaagacca aagatataac cggagggctt
      721 ccgcgcgttg ccgagctctt tgaggcgcgc aagccgaagg aatttgccgt tatcagtgaa
      781 atcgacggag tggtttcatt cggtaaagat accaagggca aacggaaggt tatcgtcacg
      841 ccggaggtcg gtgagcccag agaatatctc attcccaaag gtaaacacat cagtgttcac
      901 gaaggggacc gtatccgggc cggagaagcc cttatggacg ggtcttccaa ccctcacgac
      961 atcctcaata tcttaggcga gaaggctctc gccaagtatc tcgtggacga agtgcaagag
     1021 atttatcgtc tccagggcgt ccgcatcaac gacaagcata ttgaagtcat tgtgcgccag
     1081 atgctacggc gagtccgaat caaagaggtt ggcgatactg attttttggt tggagaccat
     1141 gtcgaaaagt ggcgattcga tgatgaaaac caaaaagcaa tccagcaaaa cggtaagccg
     1201 gccgtcgcag aacctcttct tttaggaata accaaagcct cactttcgac agaaagtttt
     1261 atctcggcgg cgtctttcca ggaaaccacg aaagttctca ccgaagcggc gattcacggg
     1321 aagatcgatc ttctctgcgg cctcaaagaa aacgtaatca tgggacggct gatccccgcc
     1381 ggtacgggcc tggcgagata ttccaaggcg gagatcgaaa tcgaagcgcc agagggcggc
     1441 gacggggaaa ttccccaggc cgcggaggca taaccccttt gacaacggtt ggttaatttg
     1501 ttaattagaa agtttttccc agagagaaag aagtgttcga cattgtgagt cgcgattgaa
     1561 tacacgctat gcctacgatt agtcaattag tccgcgagag cagagagaag caacgacgca
     1621 agaacaccgc gcccgccctg cagggttgtc ctcagaaacg aggggtgtgc acacgtgtct
     1681 acacatccac acccaagaag ccgaactcag cgttacgaaa ggtcgcccgc gtccggctca
     1741 cgaacggaat cgaagtaact tcttatattc ccggcgtcgg acataatttg caagaacatt
     1801 ctgtcgtgct tatccgagga ggtcgcgtca aagatctccc cggcgttcgc tatcacatca
     1861 ttcgtggaac tttggattcc attggggttc aggaaaggcg acaagggcgt tccaaatacg
     1921 gagcgaagag accgaaatag agcaacatgc cgcgcaaagg ggaagttaga aaaagagagg
     1981 gtttgccgga cccaaaatat ggggacaaac tggtagctaa gtttgttaac acgatcatga
     2041 gtcggggcaa gaaaagtgtg gcggagtcga ttttctatcg ctcgctgaac atcgtcacgg
     2101 caaagactaa agaagactcc ttggggattt ttaaaaaggc gattgaaaat gcccgcccca
     2161 cggtagaggt tcgttcgcgc cgcgtgggcg gtgcgactta tcaagtgccc atcgaagtga
     2221 gaccccaacg acgtctttcg ctgagcatgc gttggctgat ccagtcggcg cgtgagcgcg
     2281 gcgagaaatc gatggaagag aaattggccg cagaatttat cgatgcggcg aataatcggg
     2341 gcacggcaat taagaaaaag gaagataccc atcggatggc ggaggcgaat cgcgccttcg
     2401 ctcattaccg gtggtaagta aggttagaag tttggcagaa aaaaggaggc ggttgaaccg
     2461 cctccttttt ttccggtggt agagcgaaat ggcgagaacg gttccactag agaaaacgcg
     2521 caatatcggc attatggcac acatcgatgc cggcaaaaca accaccactg agaggatcct
     2581 gtattacacc gggatcaatt acaagatcgg tgaggtgcat gagggcaccg ccaccatgga
     2641 ctggatggtt caagagcagg aacgcgggat tacgattaca tcggcggcga cgacctgttt
     2701 gtggcgcgac catcgtgtca atattatcga cactcctggc cacgttgatt ttaccatcga
     2761 agtggagcgt tcccttaggg tgcttgacgg ggcggtcgcg gttttttgtt ccgtcggtgg
     2821 agtcgagccc caaactgaaa ctgtttggcg ccaggccgac aaatacggtg ttcctaggat
     2881 cgccttcgtc aacaagatgg atcgcctcgg ggctgacttc taccgagtgg tcaagatgat
     2941 cgaggatcgt ctgggagcgc gaccggtggc gttacaattg ccgattgggt ctgaagagaa
     3001 attcgctgga atcatcgact tagtccagat gaaggcagtg atctgggaag acgagagttt
     3061 aggggccaaa ttccgggtcg aagcgattcc ggaaagtatg atcgccgagg cccggcagta
     3121 tcacgacaag cttttggaag ccgtggccga ctgtgacgag agcatcatgg agaaatacct
     3181 cgagggaaag gagatcagcg agagcgagct gcgccgggct atccgcaacg cagcccttgc
     3241 catcaagatc gtcccagtaa tgtgtggctc tgctttccga aacaaaggtg ttcagccgct
     3301 gctcgatgcc gtggtggact accttccgtc gcctttggat atacccccgg ttaaaggtat
     3361 cgatccaaac tctcaggcgg cggaagaacg tccagcaaaa gatgatgcgc cattctcagc
     3421 cctcgctttc aaaatcatga cagatccatt cgtcgggacg ttgagcttct ttcgggtcta
     3481 ttccggttcg ttgacttccg gtgcgagcat ctacaattca accaagagca aacgagaaag
     3541 aatcggtcga ctcctcaaga tgcacgccaa caaacgggag gagatcaagg aggtttacgc
     3601 gggtgatatc gccgctgcgg tgggactaag aacagccacg acaggggata cgctgtgcga
     3661 tgaaagccat ccgatcattc tagaatccat tgattttccc gatccggtta tctctattgc
     3721 gatcgagccc aagagcaagg cggatcaaga gaggctgggt ctttcgcttc aga
//
LOCUS       DASSWZ010000296         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_394118, whole genome shotgun sequence.
ACCESSION   DASSWZ010000296 DASSWZ010000000
VERSION     DASSWZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394118"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VFN58_07155"
     CDS             complement(<1..1233)
                     /locus_tag="VFN58_07155"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET8917804.1"
                     /translation="MPGKCITWFIVFSHVSSIALPFNGQMVRQAQAAEPTTPPQAQPP
                     PGAVPPAQPPVAPGPQGTTPAQPGGAPSRPERTLLPPPAVASGGMVSLNFNRADLVEI
                     IHILAQHLRLTYTIDPEVKGTVTINSAEPLRTEDLLPIFHQVLRMNGAVAVRAGNIYR
                     IMPIKDGKGLARPMGAGREDSYALQIVPVRFFSVAEMKKVLTPFVMPGGEIIENPRGN
                     FLVIMDLPSNIQRLVEIADLIDVQVFAGTRMEIYQPKVASAEELATEMTKVMQAYAAS
                     VPQTESFTAQFLALPRINQLLVISHSEAAWTYAKRWLDRIDVIAEGPGRRIFVYPVEN
                     GKAEELANVLSAALGLPAPPGGGQRRTLEELHRSTPGGTGQFGGGGQFGGSRSGSSGT
                     SGFGSSGFGSQQPFGAYAA"
     gene            complement(1263..1919)
                     /locus_tag="VFN58_07160"
     CDS             complement(1263..1919)
                     /locus_tag="VFN58_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917805.1"
                     /translation="MLTKTYWLWTTGPWEIPNPGKAKSPIFVEGSKGVTNPPAIVGTE
                     TIVSKNIFDPERGAGFTREAEANSQAFQRIRSMVLLGTAILGNNRFAILQDGATSSGA
                     PAVPGQSAATMRIKLGDTVEGFRLSEVSEKRVVFARGASTVEVPLDYFRKTDVAQPRR
                     PVAAQTGVVGPTPAQTGAVGPAPAPRVVTPGPTNVPGQRVPRAIPALPRRDRESSKED
                     "
     gene            complement(2176..>2469)
                     /locus_tag="VFN58_07165"
     CDS             complement(2176..>2469)
                     /locus_tag="VFN58_07165"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HET8917806.1"
                     /translation="AKISIQMEIGGQIDQLASLIRGIENSPKLLMVDEINVRSLFRPA
                     GFPQPQGVPQLAAQNLRVSLTVSGLARSQAPAATKSESAPIQSKGNSAKTSEP"
BASE COUNT          526 a          735 c          663 g          545 t
ORIGIN      
        1 cgcggcgtac gcgccgaaag gctgttgcga gccaaaaccg ctactcccaa agccagaagt
       61 tccagagctc cccgaccggc tcccgccaaa ctggcctccg ccgccaaact gacctgtacc
      121 cccgggagtc gagcgatgga gttcctccaa ggttctcctt tggccgccac cgggtggggc
      181 gggtaaacca agagccgcac tcaagacatt cgccagttct tcggccttac cattttctac
      241 aggatagaca aaaattcgtc gccccgggcc ctcggcaatc acgtcgatcc ggtccagcca
      301 cctcttggca taagtccaag ccgcctcgct gtgactgatc accagaagct ggttgattcg
      361 tggcagagcg aggaactgcg cggtaaagct ctccgtctgg ggtacggacg cggcatacgc
      421 ctgcatgact ttggtcatct cggtggcaag ttcttcagcg ctcgctacct tgggctgata
      481 gatctccatg cgcgtgccgg caaaaacttg gacatcgata aggtcggcga tctccaccaa
      541 ccgctgaata ttcgaaggca aatccatgat gacaaggaaa ttcccccgag gattttcgat
      601 gatctcacca ccgggcatga caaacggtgt caacactttt ttcatctcgg cgacggaaaa
      661 gaaccgcacc ggcacaatct gtaaggcata actatcttcc ctgcccgcgc ccatcggtcg
      721 cgccaggcct ttgccgtctt tgatgggcat gatccgataa atatttcccg ctctaaccgc
      781 caccgcgccg ttcatgcgca gcacctggtg aaaaattggc agcagatcct cggttcttag
      841 gggctcggcg ctgttgattg taaccgttcc cttcacctca ggatcgatag tgtaagtaag
      901 ccgcaggtgc tgagcgagga tgtgaatgat ctcgaccaaa tccgcgcgat taaaattaag
      961 cgaaaccatc cccccggaag caactgcggg aggcggtagc agcgtgcgtt cgggccggct
     1021 gggagcgccg ccgggttgcg ccggtgtggt cccttggggc cctggagcaa ccggaggctg
     1081 agccggcggc accgcccctg gcgggggctg ggcttgtggg ggagttgttg gctctgcggc
     1141 ctgagcctgc cgaaccattt gcccattaaa aggcaacgct atacttgaga catgagagaa
     1201 gacaatgaac catgtaatgc attttcctgg cattattcct cctgccgtcg ccaacggtct
     1261 tctcaatctt ctttacttga ctcccgatct cgccgcggca acgcggggat cgctctgggc
     1321 actctttgcc ctggcacatt tgtcggtccg ggtgtgacga ctcgcggtgc cggcgccggt
     1381 cccacagcac ccgtttgtgc cggcgtcggt cctacaacac ccgtttgtgc cgccaccggt
     1441 cttcgcggct gagcgacgtc ggttttgcga aaataatcca agggaacctc taccgtcgag
     1501 gcacccctgg caaaaacaac tcttttttcc gaaacttccg acagcctaaa tccctcgacc
     1561 gtatccccga gtttgatgcg catcgtcgca gccgattggc cagggactgc cggtgctcca
     1621 gaagaagtcg ctccatcctg caagatggca aaccggttgt tcccaagaat ggcggtgccc
     1681 aataggacca tgcttcgtat gcgttgaaaa gcctgggagt tcgcctcagc ctcccgggta
     1741 aaacccgcgc cgcgttccgg gtcgaaaata ttcttgctga caatggtttc cgtcccgacg
     1801 atcgcaggcg gattcgtaac tcctttggaa ccctcgacga agatcggcga tttggccttg
     1861 cccggattcg gtatttccca agggccggtc gtccacagcc aataggtctt cgtcagcacc
     1921 aatcctatgg cgactattaa gaaaacgcta agcagtgaag atcgcgtcaa aataacttaa
     1981 ttcctaccaa ttcgggttga aaaagacccc tattttactt ttaccgaact tgcgcgtcaa
     2041 atcggcgctc atttatcagc aataggttct gaaattcaaa gcgggtttct gccagtgctg
     2101 aatcaaaacc accaccgttt tcatcggttc gtaacgtctg cgcacacaat gccggcaacc
     2161 acaaaagact ctctctcagg gttcactggt cttggccgag ttcccctttg actgaatcgg
     2221 tgcggactcc gacttagtag ctgcaggcgc ctgggaccgt gcaagtcctg agaccgttag
     2281 gcttacgcgt agattttgcg cggctaactg agggacgcct tgtggttgag ggaaccccgc
     2341 tggccggaaa agggagcgca cattgatttc gtcaaccatc agcagcttgg gggaattttc
     2401 gatgccgcga attagactcg ccagctggtc gatttggcca ccgatttcca tctggatgga
     2461 aatcttggc
//
LOCUS       DASSWZ010000297         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_181632, whole genome shotgun sequence.
ACCESSION   DASSWZ010000297 DASSWZ010000000
VERSION     DASSWZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181632"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1189
                     /locus_tag="VFN58_07170"
     CDS             131..1189
                     /locus_tag="VFN58_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917807.1"
                     /translation="MRTKFLVKEFLIVGLAHFTVLPGTLFSQSDFFKGKTVTIIQGRD
                     PGGTGDMRVRAMIPFLQKYIPGNPSIVSEYMAGGGGRKAANHVYRSTRPDGLMIGNPG
                     LGMVAAAVLGESGVLYDLDKFFYLGSPYSSYHPLFLSRKQAGLSSIEKLTAASGVRIG
                     GQSVGFSTYNEGRLFAYILGLKDPKFIAGYGGPEIDQALMRGEVDARATAADSVAQRN
                     PDWIDGSLVDFHAILEIPKGDKHPQFKQLPELESFARSDRDRKVIILSRAFRVAGGPS
                     VVPPGTPKDRVAILQEAFRKTYKDPEFLREYKKLTGDEASPLLPEDHEKTIKEIPREP
                     EIIELYKKIAGAGPLPPR"
     gene            1211..2266
                     /locus_tag="VFN58_07175"
     CDS             1211..2266
                     /locus_tag="VFN58_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917808.1"
                     /translation="MAIKIMTRILCCAFLIQLVLLPDFLYSQSDFYRGKTIRIVHGRD
                     AGGSGDLRVKALVPFLQKYIPGNPTIVNEFMPGGGGRKAANYIYGSAAPDGLTIGNAG
                     SGMVSSAVLGETGVQYDIDKLIFLGSTYSSTHYFFLTRRETGLTTLEKLRSVPGVRVG
                     AQSVGHTIYNIGRLFAWVLPLKDPKFVTGYSTPERDVAFMAGELDALGTTDDHLVRNP
                     EWISANKIDLHALMEVPRGNKYPRFGHLPEIETFAKSEKERKVLAMFRNLRLTGSPFF
                     ASPRTPGDRIEILKQAMTKSLKDPEFYKEHLKLVSEEPTPLLPEENQKAIRELPRDAE
                     TIALFNKLAGPQPLPSR"
     gene            2278..>3170
                     /locus_tag="VFN58_07180"
     CDS             2278..>3170
                     /locus_tag="VFN58_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917809.1"
                     /translation="MRRGCAVLTFAVAVLARLVILPDSLFSQTDFYQGKTVTVIISTA
                     PGGTGDLRIKALLPFLRKHIPGNPTLVAEYMDGGGGRKAANYVFRNVRADGLTVGAMG
                     SGVVGLHIMRESGVMYDIDKFIYLGAPETVGHQIIYTRKDLGLSNLEKLRAKTGIRIG
                     AQSVGHVSYIAGRFFAYFLDLKEPKFIAGYTAPEVDAALLRGELDARANLATSALRRN
                     PEWLEKGLMDFHAIMDVPKGSKHPRLGHLPEIESFIKSEREKKLIDMWRVFRLVGSPY
                     VLPPGTPKERVDALQEAMRKAL"
BASE COUNT          710 a          878 c          897 g          685 t
ORIGIN      
        1 ccgcttctac cggagaatca tgaaaaggcg atccgagaga ttccgcggga tccggaagcg
       61 attgagctct tcaaaaagct cgtgggcgcg gagccgctgc cgcctcgctg aatcaaagag
      121 ggagtccgcc atgcgaacga aatttttggt caaggaattt ttgatcgtag gtttggccca
      181 ttttactgtt ctgccgggca cgcttttttc acagtcggat tttttcaaag gaaaaaccgt
      241 taccatcatc caaggccggg accccggcgg cacgggagac atgcgggtga gagcgatgat
      301 tccctttttg cagaaatata ttcccgggaa cccaagtatt gtaagcgagt atatggccgg
      361 cggcggaggc cgaaaagccg caaatcatgt ctaccggtct acccgccctg acggcttgat
      421 gatcggcaac cccgggcttg gcatggtggc tgccgcggtg ctcggagaat cgggagttct
      481 ttacgatctc gataagtttt tctatctagg atcaccctac agctcgtatc acccgctctt
      541 tttaagcagg aaacaggcgg gcttgagcag catagagaaa ctcacggccg cttccggagt
      601 caggatcggc ggtcaatccg tgggtttctc tacctacaat gaaggccgcc tcttcgccta
      661 cattctcggt ctcaaggacc ccaaattcat cgccggctat ggagggcctg agatcgacca
      721 ggcgttgatg cggggtgaag ttgatgctcg ggccaccgcg gctgactcgg tggcgcagcg
      781 gaacccggac tggatcgacg gtagcctggt ggactttcat gcgatccttg aaatccccaa
      841 gggggacaag catccccagt tcaaacagct tcccgagctg gagagctttg cccggtcgga
      901 tagagatcgc aaggtgatca ttttgtcgcg cgctttcaga gttgccggtg gtccctccgt
      961 cgttccgccc ggaaccccca aggatcgcgt cgcgattctc caagaggcgt ttcgcaaaac
     1021 ttataaggac ccggaatttc tgcgcgaata caaaaagctt accggcgacg aggcgtcgcc
     1081 tctcctcccc gaagaccatg aaaagacgat caaggaaatt cctcgcgagc ctgagatcat
     1141 cgagctgtac aagaaaattg ccggcgccgg gcctctgccg ccgcgctaag aaagaaaggc
     1201 agactcagct atggcaataa aaattatgac gcggattcta tgctgcgcgt ttttgattca
     1261 gctggtcttg ctacctgact tcctctattc acagtccgac ttttatcggg gaaaaacgat
     1321 tagaatcgtg cacgggcgcg acgccggcgg ctcaggagac ctgcgggtaa aagcgctggt
     1381 gccgtttctg caaaaataca tccccggaaa tcccaccatc gttaatgagt tcatgccggg
     1441 aggcggcggc cgcaaagccg ccaactatat ttatggatcg gcggcgcccg atggattgac
     1501 catcggaaac gccggcagcg gcatggtgtc gagcgcggtt ctcggggaaa ccggggtgca
     1561 gtacgacatc gataagctaa tttttctcgg atcgacctat agttccactc attatttttt
     1621 cttaacccga cgggaaaccg gtctgacgac cttggagaag ctgcgctcag tcccaggcgt
     1681 gcgcgtcggg gcgcaatccg tcggccacac tatttataat attggcaggc tcttcgcttg
     1741 ggtcctgcct ctcaaagatc ccaagtttgt taccggctat tcgacccctg agcgcgatgt
     1801 tgctttcatg gccggagagc tcgacgctct tggcaccacc gacgatcatc ttgtgcgcaa
     1861 tcccgaatgg atatcggcga acaaaattga tctacacgcc ttgatggaag taccgagggg
     1921 aaacaagtat ccccgctttg gtcacctgcc cgagatcgaa actttcgcta agtccgagaa
     1981 agagcgaaag gtgctggcga tgtttcgtaa tttgcggctt acgggttcgc cgtttttcgc
     2041 ttctccacga acgcccgggg atcggatcga aatcctcaaa caggcgatga ccaagagcct
     2101 caaggaccct gagttttaca aggagcacct aaagctcgtc agcgaggagc ctaccccgct
     2161 tctgccggaa gaaaatcaaa aagctatccg ggaattgcct cgcgacgccg aaaccatcgc
     2221 gctttttaac aagctagccg ggccgcagcc gctcccgtcg cgctgaggag atactcgatg
     2281 cgaaggggat gcgcagttct gacgtttgcg gtcgcagtac ttgcccgtct ggtcattttg
     2341 cctgactcac tgttttcaca aactgacttt taccaaggca agactgtgac ggttattata
     2401 agcaccgctc cgggtgggac cggcgatctt cgcattaagg ccctgcttcc ttttctgcgc
     2461 aaacatattc cgggaaatcc cacgctcgtg gctgaatata tggacggcgg cggcggccgc
     2521 aaagcggcga actacgtatt ccgaaacgtg cgggctgacg gactgaccgt gggcgcaatg
     2581 ggcagcggcg tcgtgggcct gcacatcatg cgcgaaagcg gcgtgatgta tgacatcgac
     2641 aaattcattt acctcggcgc gccggaaact gtcggccacc agatcatcta caccagaaaa
     2701 gatctgggtt tgagcaatct ggaaaagcta cgggcaaaga ccggcattcg gatcggcgcc
     2761 cagtcggttg ggcatgtctc ctacatcgcc gggcgttttt tcgcctactt tctcgatctg
     2821 aaggagccga agtttatcgc cggctatacc gcgcccgagg tggatgccgc cttgctccgc
     2881 ggcgagctgg atgccagagc aaatctggcg acctcagccc tgcggcgcaa cccggagtgg
     2941 ctggaaaaag gtttaatgga tttccacgcc attatggacg ttcccaaagg gtccaagcac
     3001 ccccgcttgg gtcacctacc tgagatcgag agctttataa aatcggaaag agaaaagaag
     3061 ctgattgaca tgtggcgggt cttccggctg gtgggctcgc cctacgtgtt gccgccggga
     3121 acgcccaagg agcgcgtcga tgctctccag gaagcgatgc gcaaggcgct
//
LOCUS       DASSWZ010000298         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_620188, whole genome shotgun sequence.
ACCESSION   DASSWZ010000298 DASSWZ010000000
VERSION     DASSWZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620188"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..643
                     /locus_tag="VFN58_07185"
     CDS             233..643
                     /locus_tag="VFN58_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917810.1"
                     /translation="MSHNTSIPATAPAPSLVTVTHLVYALHALSLLIGVTTAASIIGA
                     FVFGVPSIIAVVINYVKRGEARGTYLESHFRWQIRTFWFALLWSIIGGMLFITLVGIP
                     LAIGVFFAAGVWAIYRVARGWLALRDQKPMYTNR"
     gene            complement(696..1325)
                     /locus_tag="VFN58_07190"
     CDS             complement(696..1325)
                     /locus_tag="VFN58_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917811.1"
                     /translation="MKLVKTPLLGLLGIVFVTLALNGCSTATPPIVSEWRNPAYASGP
                     FKRIMVGGPSGQTSVRRNFEDEFVVQLRAVGVDAMPSYQYLSEDEIDENKLKEAAQKA
                     RANGVLFVRSVKTEQKTNYGTGFPNVSFGIAGPNVGAAWSGLPVASGPYRYTEYTLDT
                     ALYDGAKNELVWSGTTRTAEPENVQTAIKSYVEAVIKALDAQNLLPGPQ"
     gene            1614..2744
                     /locus_tag="VFN58_07195"
     CDS             1614..2744
                     /locus_tag="VFN58_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917812.1"
                     /translation="MPAWIIVARDFLCVCLFWVLIFAPAVYSQPSKTAGPAEWDKLVD
                     AAKKEGKVTVSLPASAELKRQIEDQFKKRYDIEVETFTSRGSAGVRRMADEFKAGVRH
                     FDLHIGGSSSIISGMLDEGILDPIEPWLVLPEVKDPKQWWGGHLWVDNAKRFVYTFQA
                     YLSEIIWYNTDLVKPNEIRSYDDFLNPKWKGKIGYLDPRTPGAGDSTWSFMWRVKGEE
                     YLKKLVAQDLYLGRDQRLLAESLAKGRVAVMIGLSYYSYLPFLKAGLPIKTLPTLKEG
                     NYGTGGSGNLAIIKAPAHPNSTKIFVNWLLGREGQEIVSRALSQATRRLDIDTHFLRE
                     SGTIAAKDVMSVNDFLQIENQSEEKLDKVREPASKVAHALLK"
BASE COUNT          686 a          803 c          779 g          683 t
ORIGIN      
        1 tccgcggctg tctgtcccat tggtagaatt tcactgcggc tgtcgccatt gttactcgcc
       61 tgtcatttta cttagccatt aataagattg gaaaccctac gtcggattgg gtcgagggtt
      121 atgactcgaa gcccttttcg gctgcaagac gtggtgtggt atagagtaca gactcagact
      181 tgagcccaaa aattttccct gtcaaagtaa tttagctgag agaggagcgc tcatgtccca
      241 caacacctcg atccccgcca ctgcgccggc acccagcctc gtcaccgtta cgcaccttgt
      301 ctatgccttg cacgccctga gccttctgat cggcgtaacg accgcggcga gcatcatagg
      361 tgccttcgtc tttggtgtac cctcgatcat tgccgtcgtc atcaactatg taaagcgggg
      421 agaggcgaga ggcacctacc tggagtcgca ctttcgctgg cagatccgga cgttctggtt
      481 tgctctcctc tggtctatca tcggcggcat gctgttcatc actctcgtgg gcatcccgct
      541 tgcgataggg gtttttttcg cggccggcgt atgggccatc taccgggttg ctcgcgggtg
      601 gctggcgcta cgagatcaaa agccgatgta tacgaaccgc tgattgccga tcacaggccg
      661 gttgccgccg ctttctcgac gagcagcacg ctcgctcact gcggtccggg aagcagattc
      721 tgcgcatcga gcgcttttat gaccgcttcg acataggact tgattgccgt ttgcacattc
      781 tccggctcgg ccgtcctggt ggtgccgctc cagaccagtt cgttcttggc cccgtcgtac
      841 agcgcggtat ccaaagtata ctcggtgtaa cggtaaggac cgctggcaac ggggagccca
      901 gaccaggcgg cgccgacatt tgggccggcg ataccgaaag aaacattcgg aaatcccgtg
      961 ccgtagttgg tcttctgttc tgtctttacc gatcgcacaa acagcacccc attagcgcga
     1021 gccttttgcg cggcttcttt gagcttattt tcatcgatct cgtcctcaga aaggtactga
     1081 tagctcggca tggcgtcgac gcctactgcc cgaagctgaa ccacgaattc atcttcgaag
     1141 ttgcgccgca cactagtttg tccgcttggg cctccgacca tgattcgctt gaagggaccc
     1201 gaggcgtaag ccggattgcg ccattcgctg acaatcggag gagtcgcggt cgaacaaccg
     1261 ttgagagcta gcgtgacaaa gacaattcct aacaggccca aaagcggcgt tttcacaagc
     1321 ttcacgcctc catgatcgcg ctaaatcgat tcttattaaa gacctccccc gcgatattcc
     1381 tcgcagaagt gcaaaaaggt cgttcaaaat ctccaatcga ggacaggggg attatgattt
     1441 ttctaaatct gttcaacaac ttatcgactt cccatctcac aatattggga acattagctg
     1501 caaaatgttt aacttcattg gggcaataat acttgacata tgaaatattt cttttaaagg
     1561 attatagatt gcacctactg agtgcgccat tcgtgtgtcg cggagacgaa agattgccag
     1621 cctggatcat agttgctaga gatttcttgt gtgtctgcct gttttgggtc ctgatatttg
     1681 cgcccgcggt ctacagccaa ccaagcaaaa ccgccgggcc ggcggaatgg gataagctgg
     1741 ttgatgccgc gaagaaagaa ggcaaggtaa ccgtatcgct cccggccagt gccgagttga
     1801 aaagacaaat cgaggatcaa ttcaaaaaac gctacgacat cgaagtcgaa acgttcacct
     1861 cccgcggcag cgcgggggtg cgccgcatgg cggacgaatt caaagccgga gtgcgccatt
     1921 tcgatctgca catcggcggt tcttcttcga tcatctccgg catgctggac gagggcattc
     1981 tcgatccgat cgagccgtgg ctagtactcc ccgaagtcaa agatccgaaa caatggtggg
     2041 gcggtcactt gtgggtcgat aacgcaaagc gattcgttta cacgtttcag gcgtacctgt
     2101 cggaaatcat ttggtacaac accgatctgg tcaaacccaa cgagatccgt tcgtacgacg
     2161 actttctcaa cccgaagtgg aaaggcaaaa tcggctatct cgatccgcgc acgccggggg
     2221 ccggagactc cacctggtcg ttcatgtggc gggtcaaagg cgaagagtat ctcaaaaaac
     2281 ttgtggcaca ggatctatat ctcggccgtg atcaacgctt gcttgctgaa agtttggcta
     2341 aaggacgagt cgccgtcatg atcggtctct cctattattc ctatctgccg tttctcaaag
     2401 cgggcctgcc gatcaagact ctgccgacac tcaaagaggg aaattacggc accggcggca
     2461 gcggcaatct ggcgattatc aaagcgccgg cgcacccgaa cagcaccaag attttcgtta
     2521 attggctgtt gggccgcgag ggacaggaga tcgtttccag agcgctgagt caagcgaccc
     2581 ggcgccttga tatcgacacc cactttttga gagaatccgg cacgatcgca gcgaaagacg
     2641 ttatgtcggt caatgatttc ttgcagatcg aaaatcagtc ggaagaaaaa ctcgacaagg
     2701 tgcgtgaacc cgcgtccaaa gtcgcccacg ccctcttgaa gtagcaacgc ccgcgagggc
     2761 ggctcaaaag ttacgatcga aaggggtcag caaagatggc taaaagcgcg acgacaacca
     2821 agtccaactt cgatattttg gaatcgtatt tcaagaaata tcccgacgtt cctaaagaga
     2881 cgatactcaa gcaacacatg ttgagtctcg gtcattggtt cagcgacgcg gcgcttgagg
     2941 catcagcagg c
//
LOCUS       DASSWZ010000299         7837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_422353, whole genome shotgun sequence.
ACCESSION   DASSWZ010000299 DASSWZ010000000
VERSION     DASSWZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422353"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFN58_07200"
     CDS             complement(<1..254)
                     /locus_tag="VFN58_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917813.1"
                     /translation="MHTSYSSILLFLSAGVKFNVQVTRISSKKELAKAYTIRSRVFVR
                     EQGVPEEIEMDRDDKRAIHFLAFSSGKAVGTARVVMHRGNA"
     gene            406..1533
                     /locus_tag="VFN58_07205"
     CDS             406..1533
                     /locus_tag="VFN58_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917814.1"
                     /translation="MSRADRVKDLMAAFQKTAYFQWMARQRIPVIDGYGVEDVREVEM
                     DYWPRLGGRAAFINLYGMEGVTGMYVAEIPAGGALEPEKHFYEEVICILNGQGATEVW
                     QEGGRKQMFEWGPWSLFAPPLNAWHRLINGGGQPVKFLAVTNAPLVMDVFHNEDFVFN
                     CPYTFSDRYSGADGFFNVGQKRYESGMQHIWETNFIMDIQSAALDQREVKGAGVRITQ
                     FELSGNGLIGHLAQWPAGRYHKAHYHGPGAILLGLQSSGYVLIWSKELGIEPYRSGRG
                     EGVVEVKWKEGSVYCPPGGWFHQHFNTGGQAARHLAIRYGSRIHPIGFKIADKRSEDG
                     VYIDVKRGGTLIEYADEDPYIRKHYEDEMNKAGVPCEMPNL"
     gene            complement(1559..2686)
                     /locus_tag="VFN58_07210"
     CDS             complement(1559..2686)
                     /locus_tag="VFN58_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917815.1"
                     /translation="MFFQKRYCFIWLALAALFLSGSVGDLLAQSDWKKEWERTLQQAK
                     KEGKIVVGISARAELRKELETVFEPQFGVDMELLTARGSQHASRIASEYQAGVKYFDA
                     FIGGSGAYESLVYDGMVEPLAPNLILPEVREEKYWWGGHIWEDNVKTKRFLYSFIADA
                     GAGGFWYNTEVAKPEELRSLDEFLNPKWKGKIGFLDPRTPGSGQSIWSFLWDVKGESY
                     LRKLVQQDLFVSRDQRQLADALAKGKLAIALGVSFYTLDGFIVANLPIKELPKLKEGA
                     PSSNGSGVIGIVKNPPHPNATKVFVNWLLGKQGQELYAKAMHQSTRRLDVDTKWLQEH
                     GVKAAKDVMTLEEYHRMRNHLEDKYTQVRIPSAKFAEQILP"
     gene            complement(2701..3396)
                     /locus_tag="VFN58_07215"
     CDS             complement(2701..3396)
                     /locus_tag="VFN58_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET8917816.1"
                     /translation="MIRNTEINVLSAGAVQPGMVKVIDAFRHETGHEVKVVFATAPAI
                     LSRIDRETPDIVIAPPAVLDELVKVGKVTPTDRVTVVGRIGVGLMVRGGAPLPMIGTI
                     EEFKQSLLTAKSVVYNQASTGIYLEKLFERLGIVEHLKTKTVRYPDAAAVLDHVGKGK
                     GDEIGFGATTVIIEGESKGLKFVGPLPPEIQNYTTYAAVLTAGSAQEAAREFIRFLTG
                     PVAQKVFAAAGIQ"
     gene            3678..6146
                     /locus_tag="VFN58_07220"
     CDS             3678..6146
                     /locus_tag="VFN58_07220"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET8917817.1"
                     /translation="MRKWIIIGGVLFALCAIVVLALLNLNSLINRNKEYFLGQAEQAL
                     GRKVSVGDVGVTLWGGVGLTLSNFTMSDDPSFSSSHFVSAEDLQINVKLLPLLRKDFQ
                     VKRLILHKPVIQVIRNKEGKFNFSTLGKEKKEKQKELEKVEKEKEQKTPPALLIAVVD
                     ISGGEVHYADQKAGTDFRANQIDFKVKDFDLNRPFSAELAAALFSEKQNFKLQAQIGP
                     IGSHTDFSDVPLDGKADIESLDFGKLKSTLPAVRAALPKDMDLSGILTAKDLRLKGTL
                     KKLSLKGVLDGTGAAVTFGQTLRKAAGIPLVVTADAQYGNGLLRLGQTKAKLNTMELT
                     GNGEVNLGDVPVLNLSLASNRFSLDGWEKIIPLLNDYQLSGNLEVQKTTVQGKLGKGG
                     MPQIQGSLTLSGVNAKPPQFPKPIKDLDTRIDFTGQRATLKDTTLSLGNSRIRLAAEI
                     DRFSPLTLTYRLSTPEIWPADFQAALPEDRKTDVIKNLSSEGTLSAQDGALRFRGKLA
                     STQGTLYKVDYKDLGTNLTLENKIASIQNLRLNALNGSLQAEAQYAFNSPTPRFSLVS
                     KAQGLDLRELYRSVDSKSTRDIQGRLNADMKISGSGKEWNEIKPSLRGQGQAEVLQGA
                     LLDFNIADNVLSSVTGIPGLTSLINPQVRKKYPETFEAKDTQFKELKGQFDLADARMN
                     IKDLRIAAADYTVQGNGWVDFEKRADFQAVLLLSQALSADLGRSAREVTYMFNNQNQF
                     ELPFSLTGTLPKLKARPDSNYLGKMVQRGFMRKGAEELQQRLLGKERSAPSGETAPSD
                     PKREKKKNSTEELIRKGLDQLFKR"
     gene            6396..7286
                     /locus_tag="VFN58_07225"
     CDS             6396..7286
                     /locus_tag="VFN58_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8917818.1"
                     /translation="MAKFLSIVLSAFLLSACATVTEDNGSQVAIQELPTPGAEIQPGE
                     SNVPAPAESARNEVARKIPTKQEIKVIQAQLKAAGFDPGPVDGTLGAKTTSALRRLQS
                     GCANLNDLFENPTSGILPQPGGMQAGKQSAADSASNTDEIRLIQVRLKDAGFDVGPVD
                     GVMGFKTKSALLRFQSGCTMVKDLPAIFENQVQTSDRMPSPMSASEKQIQPTSKAPSA
                     TGSVRDEAGKVNAATDKSPSKEEIRLLQAQLKAAGFDPGPFDGMLGPKTKSALQQYRT
                     VNGSSTSQKLSSNVGLKFDY"
BASE COUNT         1926 a         1956 c         2080 g         1875 t
ORIGIN      
        1 gcgttccctc gatgcatgac cacccgcgcg gttcctaccg cttttcccga tgagaaagcg
       61 agaaaatgaa tcgcgcgctt gtcatcgcgg tccatttcaa tctcttccgg aacaccttgt
      121 tctcttacga acacccgtga gcgaattgta taagctttcg ctagctcttt ttttgaagaa
      181 attctcgtca cttgcacgtt gaatttcaca cctgcgctta gaaataaaag aatcgacgag
      241 tagctagtgt gcacattatt ctcactgcca tgctacaaca agggtaacga gaacgacaac
      301 tgactggagg gcgatccaac aaaagggaga accgtctctc ctacgaagtt gacgtgagac
      361 actagttatc ggataaacag attgtgaata taggaggggg gtattatgtc acgagctgat
      421 agggtcaaag atttgatggc ggcgtttcag aagaccgcat actttcagtg gatggcgcgt
      481 caaagaatac ctgtaatcga tggttacggc gttgaagacg tgcgcgaagt agagatggat
      541 tattggccac ggctcggagg gagggcggct tttatcaatc tgtatggcat ggaaggtgtc
      601 acagggatgt atgtggcgga gattcctgct ggcggtgcgc tggagccgga gaaacatttt
      661 tacgaggaag taatctgtat cctgaacgga cagggagcca cggaggtatg gcaagaaggc
      721 ggacgcaagc agatgttcga gtgggggcca tggagccttt tcgcgccgcc gctcaatgcc
      781 tggcatcggc tgattaacgg tggtgggcag ccggtaaagt ttttggctgt taccaacgcg
      841 ccgctggtca tggacgtctt ccacaacgag gactttgtct tcaattgtcc ctacacgttt
      901 tcggatcgct actcgggcgc cgatgggttt tttaacgtcg gtcaaaagcg ctacgagtcg
      961 ggcatgcaac acatttggga aacaaatttt atcatggaca ttcaatcggc ggctttggat
     1021 cagagggagg tgaagggcgc gggagtgcgc attacccaat tcgagctgtc tggcaatgga
     1081 ttgatcggcc atctggcgca gtggccagcg gggcgatacc acaaagcgca ctatcacggt
     1141 cccggggcaa tcttgctggg cttgcagtca tcgggatatg ttttgatctg gtcgaaggag
     1201 ttgggaattg aaccttaccg aagtggtcgt ggcgagggcg tcgtggaagt aaaatggaag
     1261 gagggaagtg tttactgtcc tcccggaggc tggttccacc agcatttcaa taccggtggg
     1321 caagccgcgc gccacctggc gatccgctat ggcagcagga ttcaccccat cggctttaag
     1381 attgccgaca agcgaagcga agatggcgtc tacatcgatg tcaaacgagg cggcacgtta
     1441 atagaatacg ccgacgaaga cccgtacatc cggaaacatt atgaagacga aatgaataaa
     1501 gcgggagtgc cctgcgagat gccgaatcta tgacgcaggg gtggctggcc ggtcgctcct
     1561 agggaaggat ctgctcggca aatttggctg aagggattct cacctgcgtg tacttgtctt
     1621 ccagatggtt tctcatccgg tgatattctt cgagggtcat gacgtccttc gctgccttaa
     1681 cgccatgctc ctgaagccac tttgtgtcca catccaggcg gcgtgtcgat tggtgcatcg
     1741 ctttcgcgta gagctcctgc ccttgttttc ccaacaacca attgacaaag actttggttg
     1801 cgttgggatg tggcggattc ttgacgatgc cgatcactcc ggaaccgttg ctggacggcg
     1861 cgccttcttt tagtttagga agttctttaa tcggcagatt ggcgacgata aagccgtcaa
     1921 gggtgtaaaa gcttacaccc aaggcaatcg cgagcttgcc ctttgccagc gcatcagcaa
     1981 gctggcgctg atctcggctc acaaaaagat cctgctgcac aagttttcgc agatagctct
     2041 ctcctttaac gtcccaaaga aaagaccaga tggattggcc tgagccggga gttctggggt
     2101 cgagaaatcc tattttgcct ttccacttgg gattgaggaa ctcgtccagg gagcgaagct
     2161 cctccggttt cgcaacctca gtgttgtacc aaaagccgcc cgccccggca tcggctatga
     2221 aagaatagag aaagcgtttg gttttaacgt tatcttccca gatatggcca ccccaccaat
     2281 atttctcttc cctaacctca ggcagaatca aattcggcgc caacggctca accatcccgt
     2341 catagaccag tgactcatag gcgccggatc ccccgatgaa agcgtcgaag tatttgactc
     2401 cggcttggta ttcggaagcg atccggctgg cgtgttgtga acctcgcgcg gtgagaagct
     2461 ccatatcgac gccgaattgc ggctcgaaaa ccgtttccaa ctctttcctc agctcggcac
     2521 gcgccgagat acccacgacg atcttgcctt cctttttcgc ctgttgcaga gtcctttccc
     2581 attccttctt ccaatcagac tgcgcgagga ggtctccaac cgagccggag agaaacaacg
     2641 cagcgagagc taaccaaatg aagcaatatc tcttttggaa aaacatcggt ggctctcttt
     2701 ttattggatg cctgctgctg caaaaacttt ctgtgccacg ggcccggtga ggaagcggat
     2761 gaactcccgc gccgcttcct gagcggaccc tgcggtaagg acggcggcgt aagtcgtgta
     2821 gttttggatt tctggcggca atggcccgac gaatttgaga cctttgcttt cgccttcgat
     2881 gataacggtg gttgcgccga atccgatctc gtcgcccttg cctttgccaa cgtgatcgag
     2941 cacggctgcg gcatccggat agcggacggt cttggttttc aagtgttcca cgattcccag
     3001 gcgctcgaat agcttttcta gatagatccc ggttgacgcc tggttgtaaa ccactgactt
     3061 ggcggtcagc agggattgtt tgaattcttc aatggtgccg atcatcggca acggcgcgcc
     3121 gccacgaacc ataagcccga caccgatccg gccgacgacg gtgacgcggt cggtgggtgt
     3181 cacttttccg accttgacta attcgtctaa gacagccggc ggagcaataa cgatgtccgg
     3241 cgtctctcgg tcgatacgac taaggatcgc tggggcagtg gcgaaaacga ccttcacctc
     3301 gtgaccggtt tcatgccgga atgcgtcgat gactttcacc atccctggct gaacggcgcc
     3361 cgcgctgagc acattgattt ccgtgttcct aatcatcaag tacaaagccc taaagagagg
     3421 tccccgaatg ccggttctta aaggcaaatc atctcataga ggagaaaact tgatttagca
     3481 agcgtgaacg catttacgga agtctgtttt tattcggctt gagcttgtcc tcctgtcctc
     3541 ttcactcagc cgtgtctttc tactaaaata tactttggac cgcattgaac attcagatcc
     3601 aattcatcac ctagttggcg gacagcccgg actgaaagtc gtcgaaaacg cgtttccagg
     3661 gacaggggag atatcgtatg cgcaagtgga tcattattgg cggcgttttg tttgcccttt
     3721 gtgcgattgt ggttctggct ctgctgaacc tgaactcgct gatcaaccga aataaggaat
     3781 attttctggg ccaggcggag caggcgttag gacgcaaggt gtcggtgggt gatgtaggag
     3841 tgacattatg gggtggtgtc ggattgacgc tcagtaactt caccatgtcc gacgacccgt
     3901 ccttctcatc gagtcatttt gttagcgccg aagaccttca gatcaacgtg aagctgctac
     3961 ctctcttgcg caaggatttt caagttaagc ggttgattct tcacaaaccg gttatccaag
     4021 tcattcgaaa taaagaaggc aagttcaact tctccaccct cggtaaggag aaaaaggaaa
     4081 aacagaaaga gctggaaaag gtcgaaaagg aaaaagagca gaagactcct ccggctttat
     4141 tgatcgccgt ggtcgatatc tccggcggag aagtgcacta cgcagatcaa aaggcaggga
     4201 ccgattttcg tgccaaccag atcgatttca aagtgaaaga cttcgatctg aaccgacctt
     4261 tttctgcaga gctggcggct gccctgtttt ctgaaaagca gaacttcaag ctgcaggccc
     4321 agattggacc catcggctca cacacagact tcagtgacgt tcctctggac ggcaaagccg
     4381 atatcgaatc acttgacttt ggtaagttga agtcgacttt gccggccgtc agggcggcat
     4441 tgcctaaaga catggatctg tcgggaatac tgacagccaa ggatttgcgg ctaaagggca
     4501 cgttgaagaa gctttcctta aaaggcgttt tagacggtac tggcgctgcg gtgacctttg
     4561 gccagactct gcgcaaagct gcaggaattc cattagtcgt tacagccgac gcccaatacg
     4621 gaaacggttt gcttcgcttg ggccaaacga aagcgaaact gaatactatg gagctcaccg
     4681 gcaacggaga ggtaaacctg ggagatgttc cggtgttaaa tctctcgctt gcttccaacc
     4741 gtttttctct cgacggctgg gaaaaaatca ttccgcttct taatgactat cagctttcag
     4801 gaaatctaga ggtccagaag accaccgtcc aaggaaaact cggcaagggt gggatgccac
     4861 aaatccaggg tagcctgact ctctctggag taaatgccaa accgcctcag ttcccaaaac
     4921 ccattaaaga cctcgatacc aggattgatt tcaccggcca gagggccacg ctaaaagata
     4981 cgacactcag cttgggaaac tcgcgcatcc gtttagcggc ggagatcgac cgtttttcgc
     5041 ctctcacact cacttatagg ctttcaactc cggaaatatg gccggcggat tttcaagcgg
     5101 cgctacccga agatcgaaag acggatgtga tcaagaacct cagcagcgag ggaactttga
     5161 gcgctcaaga tggggcgctt aggtttcggg gaaagcttgc gtcaacccag gggacgctgt
     5221 acaaggtcga ttacaaagat cttggaacca acctcactct cgagaacaag attgcgagta
     5281 ttcagaatct ccgtctcaat gcgctgaacg gttccttaca ggctgaagct cagtatgcgt
     5341 tcaatagtcc gacaccacgt ttttcattag tatcgaaagc gcaggggctt gatctccgag
     5401 aattgtatcg ctccgtggac tccaagagca cacgcgacat tcaaggacgt ctcaacgcag
     5461 acatgaaaat ttccggaagc ggcaaagagt ggaacgaaat caagccaagt ttgcgcggtc
     5521 aagggcaagc agaggttttg caaggggcgc tgttagattt caacatcgcc gataacgtgc
     5581 tctccagcgt taccggcatt ccgggtctta cttcgctgat caatccgcag gtacgaaaaa
     5641 agtatcctga aaccttcgag gccaaggata cgcaattcaa agaattgaaa ggacagtttg
     5701 atttggcgga tgcacgcatg aacataaaag acctgcggat agcggccgct gactatacgg
     5761 tgcagggaaa tggctgggtc gattttgaga agcgggcgga tttccaggcg gtactgcttc
     5821 tctctcaagc gttatcggcg gatctcggac gctccgcgcg tgaagtcact tacatgttca
     5881 acaaccaaaa tcagtttgaa ctaccgtttt ctctaactgg taccctgccg aagctaaagg
     5941 ccagacccga ttcgaattac ctaggcaaaa tggttcagcg tggcttcatg cgtaaaggag
     6001 cggaggagct tcaacaaagg ttgctcggca aagagcgatc ggcgccttca ggggagacag
     6061 cgccttcgga tccgaagagg gaaaagaaga aaaattcaac ggaagaactg atccgtaaag
     6121 gtttagatca actattcaaa cgctagcttt ttagcgtctc gctcctgcgt atatttcgct
     6181 tccaaagcga gatatccact caatgtaaat cggtcccatt tgcagctttg ggccgaaacg
     6241 gtctggtgtc atttgccgac aagtctgtct tcgctggcaa gcgctcgact gggcgacacc
     6301 ccttaagttg ccatttttcc aagtcttttc ttttgattgt aaccggcata tcgcttgctt
     6361 cttgttgccg gatgcaaata gacgaggaga ctttcatggc taaattttta tctattgtgc
     6421 tatctgcttt tttgctgtcg gcgtgtgcaa ctgtaaccga agacaacggt tctcaggtgg
     6481 cgatacagga actgccaact ccgggtgcgg aaatacaacc gggcgaatcg aacgtcccag
     6541 cgcccgcaga gtccgcaaga aatgaggttg ctagaaaaat tccaaccaag caggaaatca
     6601 aggtgatcca agcgcaactg aaggcggccg gatttgatcc cgggcctgtg gatggcacgt
     6661 tgggagccaa aacgacgtcc gccctgcgtc ggttgcaatc aggatgcgcg aacttgaacg
     6721 atctttttga aaatccaaca agcggaattt taccgcaacc aggaggaatg caggcaggaa
     6781 aacaaagcgc ggctgatagt gcttccaata cagatgagat ccggctgatc caagtgcgtt
     6841 tgaaagacgc cggttttgat gtgggacccg tcgacggcgt aatgggtttc aaaacgaagt
     6901 ccgcactact ccgttttcaa tcggggtgca cgatggtcaa ggacttgcct gcgatttttg
     6961 agaaccaggt tcaaacgtca gacagaatgc cgagtccaat gtccgcttct gaaaaacaga
     7021 ttcagccaac atcgaaagct ccttcggcga caggatccgt tagggatgag gccggcaaag
     7081 tgaacgcggc aacagacaaa tctcccagca aggaagaaat ccgacttctc caggcacagc
     7141 tgaaagcggc tggtttcgat ccaggccctt tcgatgggat gctagggccc aaaacgaagt
     7201 ctgcacttca gcagtaccgg acagtcaatg gatcttcgac ttcacaaaaa ctgtcgtcga
     7261 acgtcgggct caagtttgac tattagttcg gcttagagcg gtcctatgat ctcgttaccc
     7321 cctcatagct tctagctcct aagtatcact ttctatttag tcttctaact agtcttctga
     7381 attctgcttc ttgtttttac cgactagggc tgttggtttg gtcgaatcgg ttaacctttt
     7441 cgttgatcca acgtctaaga ggttatgtat gccaaggaag ctgaggaggc ttctatgaag
     7501 gatgtaacga ccgcactgct agccagtctt ttcgtactga taacattagt ttccctgatt
     7561 tctgcagatc ctgtaccgca cggcacaaac tgggcagcgc ttactaactc gggaagtgtc
     7621 tcagagcttg aatttcgata cgccttgcga agataagccg gggttcgctg ttttcacctc
     7681 tgccagacaa tatgagattg ccaccggaac gattttgaag tcacagatac acaacaccct
     7741 ccaatcattc tgtgtttaga tccgtgccag acggatgctt ttcagcgagt tcggcttctt
     7801 gtgccgctct ggtcagagcc gggacaattt caaacag
//
LOCUS       DASSWZ010000300         6063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_960228, whole genome shotgun sequence.
ACCESSION   DASSWZ010000300 DASSWZ010000000
VERSION     DASSWZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960228"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VFN58_07230"
     rRNA            complement(<1..139)
                     /locus_tag="VFN58_07230"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(405..1787)
                     /gene="der"
                     /locus_tag="VFN58_07235"
     CDS             complement(405..1787)
                     /gene="der"
                     /locus_tag="VFN58_07235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET8917819.1"
                     /translation="MAESSALKTPLARRSPVLAIVGRPNVGKSTLFNKLLGERKAIVD
                     DSPGVTRDRNYGEAEWAGRKFQLIDTGGLDTDADGDLEENVQKQSRLALAEADVILFL
                     FDGKSGLNPLDREAVRLLHKEDKPVLFAVNKLDSRQRADNLYEFYALGIDPLFSISAE
                     HGLGIPDLMDEVVQRFPNAEESDEKTEEKAPLRISVVGRPNVGKSTLINRLLGFERSV
                     VDSTPGTTRDALDSPFKLEGEPCLLVDTAGIRRKARINDRLERFSVSHSLRSVDRGDL
                     VIHILDGVEGVTDQDAQILSYAWQRGKALLLAVNKWDVVSKNGADVESYREEVYHQLS
                     FLDFVPVTFISASTGYGVRRMLETAGGVLRAYGKKVSTSAMNQALQRIVRAHAAPLSQ
                     GKPVKFYYGTQTGTRPPTFTLFVNTPQAVAESYQRYLIHQLREHLALEYVPIRLVLRA
                     RREEATRRKR"
     gene            complement(1789..2712)
                     /gene="era"
                     /locus_tag="VFN58_07240"
     CDS             complement(1789..2712)
                     /gene="era"
                     /locus_tag="VFN58_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET8917820.1"
                     /translation="MTKENAENKGAEMGSFRSGYIAIIGRPNVGKSTLLNQLLGEKVA
                     IVTPKPQTTRNRITGIRTSPKSQIVFIDTPGIHQAHSLMNRRMVEVALQTLHEVDGVF
                     WLLDSRERIGPEEERIAGNLGRLKTPSLILLNKIDLVAKGKLLPLMQRCSELLPTKEI
                     IPISALKGEGVPLVLEIVEKWLPEGPQYFPEGEFTDQTERFVASEIIREKIFLLTHEE
                     IPYGVAVTIDEFTEKEEKNLIVIKATIITERESHKPILIGKRGSMLKEVGQRAREEIE
                     ALLGCKIFLELFVKVDRGWTQDPNALTEMGL"
     gene            2827..3390
                     /locus_tag="VFN58_07245"
     CDS             2827..3390
                     /locus_tag="VFN58_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917821.1"
                     /translation="MKKRLTRRASEMIERSLRYFPELHGKTITVGLTRKHLGSATISY
                     RAGTISGLVIRLRVRKVSYQTIGHELTHLVQGLAHGDRSAPRSVARSRIPSGEKQCDI
                     WTLARHALFCDDPPTYLRLPRVVRDQWPRYAEPVRTLCMAAIEKRPSHRLYIRWLESE
                     IRQLPRSLPEKKSGEQMELPFDPILSI"
     gene            complement(3379..4611)
                     /locus_tag="VFN58_07250"
     CDS             complement(3379..4611)
                     /locus_tag="VFN58_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917822.1"
                     /translation="MKLANPLAMGGELGVGRVRLLAIVTGGHFAIHWFQQLFPVVLPS
                     LKTNLGLSDVQVGALSSVRQLINGAMDLPCGILADSLARHRAPLLASALLSMGVGYSL
                     MGVLPVFAWVLIGCGLIGLGTALWHPAAAAALSNNFPERRATALSIHGMGATISDTLT
                     PLVAGFLLVTVSLNSFLAVQLGPALIIALLIWAGLARQFSKVEKPTALSEHLREMVAL
                     AKNSTFVGITLATGFMQMGRIVIITFLPIYLQEHLGYSPFALGVYIALLHAMGIISQP
                     ILGYLSDRLGRKAVLFPSFITLGILFALLRTVAPGIPLAFVVAAIGLFFYTLLNITFA
                     AAMDVAGSKLQATSYGLSSLMMQLATSPTPIVAGWLIGKYGIGSTFALAGFFTLIGGL
                     LLLPLRLYRGTRAESLDR"
     gene            complement(4676..5626)
                     /locus_tag="VFN58_07255"
     CDS             complement(4676..5626)
                     /locus_tag="VFN58_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET8917823.1"
                     /translation="MNYRQLGNSGVRVSVIGLGANRFGSENVPQTEVNRIIAAALDLG
                     INFIDTSNNYTAGHSEETLGHALKGRGDKVVLATKFSFPKKDGPNTWGASRYQMMQAV
                     EGSLRRLQTDHIDLYYAHRWDDTTPIEETLRAFDDLIRLGKVRYIGASAYASWQLAHA
                     NVLAELRGWTPFVVLQSEYNLLDRSVEREVLPYCRAHRVGFVPYHPLAGGFLTGKYEK
                     GKLPPAGSRGERDRYVQRCMTDRNYGIVKDLATWADSRGRKINELAQAWLLTQPQVCS
                     VITGATKAEHVLSNVDGGNWALTAEESDEVSAILKGSSED"
     gene            complement(5808..>6063)
                     /locus_tag="VFN58_07260"
     CDS             complement(5808..>6063)
                     /locus_tag="VFN58_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917824.1"
                     /translation="AGELPPIECWPELWVVDDHQQAEAEAILKKTLAPLKSVKKSWHC
                     AGCGEEIEGQFSECWKCGRSRGDEVAPLDTVVRTFSIRNR"
BASE COUNT         1407 a         1752 c         1556 g         1348 t
ORIGIN      
        1 aatcttagag gcaggttacc cacgtgttac tcacccgtgc gccactttac tcggagccga
       61 agcccctttc tcgttcgact tgcatgtgtt aggcacgccg ccagcgttcg ttctgagcca
      121 ggatcaaact ctccagttaa atctttcaat ccgcctttag gcggttgaat gactgttgag
      181 tatgatagct gtactcaaaa gctttacgga aaccctaaat cgtcactgcc tataataaat
      241 ggcagttgct actatttagt tttcaaagac cagattttct tactcagaaa tgagcaggca
      301 aactaacttt atatgtactt agctgctcgt tgtcaatacc gatccgaaga tttttctgaa
      361 aaattgtttc gttgaaaaaa tgaatcagct aggcccaaac tcgatcatct ctttctcctt
      421 gttgcctcct cccgccgagc tcgcaatact agccgaatag gcacgtactc gagcgccagg
      481 tgttctcgca actgatgaat caagtaacgc tgataactct ccgcaactgc ctgcggcgta
      541 ttgacgaaca aagtaaaggt cggcggacgc gttccggtct gtgtcccata gtagaatttc
      601 acgggtttgc cctgcgatag gggggctgcg tgagctctga cgatccgctg caatgcttgg
      661 ttcatggcgg aggtcgaaac tttcttgcca taggcccgca gcacaccgcc cgccgtctct
      721 aacatcctgc gcacgccgta acccgtcgac gccgaaataa aagtcacggg aacgaaatcc
      781 aaaaacgaaa gctggtgata gacttcttcc ctgtagctct ccacgtccgc accgttcttc
      841 gacactacgt cccacttgtt tactgccaag agcagcgcct tgccgcgctg ccaagcgtag
      901 ctcagaatct gtgcgtcttg atccgtaacg ccctccacgc catccaggat gtgaatgacg
      961 agatcgccac ggtccacgga gcggagggaa tgactcacgc tgaaccgttc caggcgatcg
     1021 ttgatccgcg ccttgcgacg aatgccggcc gtatccacca gcagacaggg ctcgccctcc
     1081 agtttgaagg gactgtccag cgcatctcga gtggtcccgg gcgtggagtc aactacggag
     1141 cgctcgaaac ccagcaaccg attgatcaga gtcgatttgc cgacgttggg ccgcccgact
     1201 acggaaatac ggagcggcgc cttctcctcg gttttctcat cactttcttc ggcattgggg
     1261 aaccgttgta caacctcatc catcaggtcg ggtattccca ggccatgctc cgccgatatt
     1321 gagaacaacg ggtcgatccc taaagcgtaa aactcataga gattatccgc cctctgccgc
     1381 gaatcgagct tgttgacggc gaacaacacg ggcttgtcct ctttatgcaa cagacgaacc
     1441 gcttctcggt ctaacggatt cagcccgctc ttgccatcga agagaaaaag aatgacatcc
     1501 gcctccgcca atgcgaggcg gctctgtttt tgaacgttct cttcgagatc tccatcagcg
     1561 tcggtatcca accctccggt gtcgatgagc tgaaacttcc tgccggccca ctccgcctcg
     1621 ccgtagttgc ggtcccgtgt gacgccggga gagtcgtcaa caatcgcctt gcgttcacct
     1681 aacaatttat tgaacagggt cgatttgccg acgtttggac ggccgacgat agccaggacc
     1741 ggcgacctgc gcgcgagcgg cgtcttcagt gccgatgact cagccatctt acaatcccat
     1801 ctctgtcagc gcattagggt cctgcgtcca accccgatcg actttgacga acaattccaa
     1861 aaaaatttta cagcctaaca gcgcttcgat ctcttctcgg gcgcgctggc cgacctcttt
     1921 cagcatagac cctcgcttgc cgattaagat cggcttatgc gattcgcgct cggtgatgat
     1981 cgttgccttg atcacaatca gattcttttc ttccttctcg gtgaactcat caattgtcac
     2041 ggccaccccg taaggaatct cttcgtgggt gagcaagaaa atcttttccc ggatgatctc
     2101 cgaagccacg aaacgttcgg tttgatcggt gaactctccc tctgggaagt actgaggtcc
     2161 ctcaggcagc cacttttcca cgatctccag aaccagcggc acgccctcgc ccttgagtgc
     2221 actgatcgga ataatctctt tagtaggcaa aagctcagag caacgctgca tcagcggcag
     2281 caatttgcct ttggcgacaa gatcgatctt gttgagcaga attaaactcg gagtcttgag
     2341 cctgcccagg ttccccgcga tcctttcctc ttcgggtccg atccgctcgc gtgagtcgag
     2401 cagccagaac acgccatcca cttcgtgaag tgtctgcaaa gccacctcca ccatgcgccg
     2461 gttcatcaag gagtgagctt gatgaatacc gggggtatcg atgaagacga tctgcgattt
     2521 cgggctggtc cgaatccctg taatccgatt gcgggttgtc tgtggcttgg gagttacgat
     2581 ggcgaccttc tcgcccagta attgattgag cagggtcgat tttcctacgt tggggcgccc
     2641 tatgatggca atatagccgg agcgaaacga ccccatctcc gcgcccttgt tctcggcgtt
     2701 ttcctttgtc ataagtttca cttagacgaa acaacattat gtcacgcccg actcactgtt
     2761 caagattgca agtcgctact caccacaggg attaattaga tcgtgccgct cgactttaca
     2821 ccactcatga aaaagcgcct tacgcgtcgc gcgtctgaga tgatcgagcg ctcgctgcgc
     2881 tactttccag aattgcacgg caagacgatc acagtgggat tgacgcgcaa gcatctgggc
     2941 agcgcgacta tcagttatcg tgctggcact atctcaggtc ttgtcattcg actcagagtt
     3001 cgcaaggtaa gttatcaaac catcggtcat gaactgacgc atctcgtcca gggtttggct
     3061 cacggggacc gatcggcgcc gcgctcggtg gcgcgatcca ggattccgtc gggagaaaag
     3121 cagtgtgaca tctggacctt ggcgcggcat gccttgttct gcgacgaccc gccgacttac
     3181 ctgagactgc cacgcgtcgt tcgagatcaa tggccgcgtt acgctgagcc cgtccgtacc
     3241 ctttgcatgg ccgccataga gaaacggcca agccaccgtc tctatatccg ttggcttgag
     3301 tcggaaatcc gccaactccc ccggagttta ccggaaaaaa aatccggcga gcaaatggaa
     3361 ttgcccttcg atcccatctt atctatctag tgattcagct cgtgtcccgc gatatagtct
     3421 taagggcaaa agcagcaagc cgcctataag agtaaaaaaa cccgccagag caaacgtaga
     3481 gccgataccg tatttgccga tgagccaacc cgccacgata ggcgtcggag acgtagcgag
     3541 ctgcatcatg agtgaagaaa gcccgtagga cgtagcctgg agcttggaac ccgcaacgtc
     3601 catggctgct gcgaaagtaa tattgagcaa ggtataaaag aagagaccga tagccgccac
     3661 tacgaatgcc agcgggatgc ccggcgctac cgtcctaagc aaggcgaaga gtattcctaa
     3721 agtgatgaac gagggaaaca gcacggcctt gcgacccaaa cggtccgaga gatagcctaa
     3781 gatcggctga gaaatgatcc ccatcgcatg aagaagggca atgtaaaccc cgagagcaaa
     3841 cggcgagtag ccgagatgct cctgaaggta gatgggcaag aatgtgataa tcactatccg
     3901 tcccatctgc atgaaacccg tggccaaggt gatgccaaca aacgtcgagt tctttgccag
     3961 cgccaccatt tctcgcaggt gctcggaaag ggcagttggc ttttccactt tgctgaactg
     4021 tcgcgctaga ccagcccaga tcaggagcgc gattatcaaa gccggcccca gttgcacggc
     4081 taaaaaactg ttcaacgaaa cggttaccaa aaggaaccca gcgacgagcg gagtgagggt
     4141 gtcgctgatt gtcgctccca tgccgtggat cgacaacgcc gtcgcacgcc gctcaggaaa
     4201 attgttagaa agcgcggcgg ccgccgccgg atgccaaagc gctgttccca acccaatcaa
     4261 accacacccg atcaagaccc aagcgaagac cggaaggact cccatgagag agtaacccac
     4321 tcccatggaa agtagcgccg aagctaaaag cggggctcgg tggcgggcaa gtgagtcagc
     4381 taagatgccg caaggcaaat ccatcgctcc gttgatgagc tggcgcactg agctcaatgc
     4441 acccacctgg acatcgctta accctaagtt ggttttaaga gaggggagaa cgacggggaa
     4501 gagctgctga aaccagtgga tagcgaaatg gccgccggtg acaatagcta agagtctaac
     4561 tcggcccaca cccaactctc cccccatcgc caatggattt gctagtttca cgctcgagct
     4621 gattcgcttc tgatccagct tcatcactcg gagaggtgga gaagtgtttt cacggctaat
     4681 cctcgctgct tcccttgaga atggcgctca cttcgtcgga ctcttccgcc gtaagagccc
     4741 aattgccccc atcgacattg ctcaaaacat gctccgcttt cgtcgcgccg gtgatcaccg
     4801 agcagacctg tggctgcgtc aacagccatg cctgagccaa ctcgttgatc tttcggcctc
     4861 gactgtcggc ccaagtcgcc aggtccttga cgatgccgta gttgcggtcc gtcatgcatc
     4921 gctgcacgta acgatctctc tcgccacggg agcccgctgg cggtagcttt cccttctcat
     4981 acttgccggt taagaacccg cccgccaacg gatgataggg aacgaagccg acacgatggg
     5041 cgcgacaata gggtaagacc tctcgctcta cgctgcggtc tagcaaattg tattcggact
     5101 gcagcacgac aaacggggtc cagccgcgca gctcggctag gacattggca tgcgccaact
     5161 gccaggaggc ataagccgac gcgccgatgt agcgcacctt gcccaagcgg atcaggtcat
     5221 caaaagcgcg gagtgtctcc tctatcggcg tggtgtcatc ccaacgatgg gcataataga
     5281 ggtcgatgtg atctgtttgc aggcggcgca aactgccctc gaccgcctgc atcatttgat
     5341 aacgcgacgc gccccaggta ttgggcccgt cctttttggg gaatgaaaac ttggttgcaa
     5401 gaacgacttt atccccgcgt cccttgagcg cgtggccgag ggtctcttct gaatggcccg
     5461 cggtatagtt gttggaggta tcgataaagt taattcctaa gtcgagcgcc gcggcgataa
     5521 tccggttgac ctcggtctgc ggcacgttct ctgagccgaa gcggttagct ccgagtccga
     5581 tgaccgaaac gcgaacaccg gaatttccga gctgtcgata attcatatgg cactcccttg
     5641 tcttccaaaa gatttttttg cattcggcct gcgaacccgt cgcaggtttg attgtatgat
     5701 tttcgtggct gagatgacaa ccaagagcga aacgaaagcc gaggaatcag taagtacact
     5761 tcgaggaaga gactaactcg tcccactctc agtcggagct agcagatcta tcgatttcga
     5821 atagagaaag tgcgtacgac agtgtcgaga ggcgcgacct catcaccgcg gcttcgaccg
     5881 catttccaac actctgagaa ctgtccttcg atttcttcgc cacagccggc gcagtgccat
     5941 gatttcttta ccgactttag aggcgccagt gtcttcttta gaatcgcttc agcttcagct
     6001 tgctgatgat catccacaac ccacagctcc ggccagcatt cgatgggagg caactcaccc
     6061 gcc
//
LOCUS       DASSWZ010000301         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_68638, whole genome shotgun sequence.
ACCESSION   DASSWZ010000301 DASSWZ010000000
VERSION     DASSWZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68638"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFN58_07265"
     CDS             complement(<1..364)
                     /locus_tag="VFN58_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HET8917825.1"
                     /translation="MESKLEQLKDALRKTEGVMVAFSAGVDSTFLLTIAHMVLGERAI
                     ALTASSATIPPGELAAAQEFAAGLGIRHIVLDSHELANPSFTQNPANRCFFCKDELYK
                     ICREQADQLGIVIIMDGTN"
     gene            complement(349..1284)
                     /gene="cysK"
                     /locus_tag="VFN58_07270"
     CDS             complement(349..1284)
                     /gene="cysK"
                     /locus_tag="VFN58_07270"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET8917826.1"
                     /translation="MNGARSILDLIGSTPVVRLRTLPGKNDAEVWAKMESFNPGGSVK
                     DRICLNMIAVAESEGKLKPGATIVEPTSGNTGIGLALIAAVKGYRLILTMPDTMSEER
                     RSLLSAYGAHLILTPDTKGMGGAIHKAEELLQEHSEYFMPQQFNNPANPDMHRKTTAV
                     ELLKQFKRIDAFVAGVGTGGTITGVGEVLKDKMKNVHVCAVEPAASPVISGGEPGYHK
                     IQGIGAGFIPAVLNRDILDEVIPVSDEDAAICARRLAAQEGLLVGISSGAACCAALRV
                     AKALGKDHVVVTVFADKGEHYLSTDLFSEKRWNQS"
     gene            1283..2419
                     /locus_tag="VFN58_07275"
     CDS             1283..2419
                     /locus_tag="VFN58_07275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET8917827.1"
                     /translation="MLATDMLGFPVEEQFEPARKEWYIFDISKSTKLTSSIMSMPAAK
                     RALVIFPGALGDFLCFLPTLRFLSQNKEVDLLARSEFADLVPPTVKVGSLERYEIRRL
                     FVSGSAREERLREFFDSYSSVFSWMGSGQRTFVQELDALSRGRARVFPFQPALMQLHQ
                     SEYYLSCVGDPALETGKLEIPLKPEAVAWSERYWQQHFLKGKLVLLLAPGSGAREKNW
                     PDASFRVVADWWRRACGRVVVILGPVEEEKGDYTALCEGAVVARSLNLGKLAALLVRS
                     DLYLGNDSGVSHLAAALGVATAVLFGPSNVGRWAPRGKNVTVVTQNADCAPCTVSTMK
                     RCPHRQCLTTLEPEYVIKKLAGLAEQLTLTRGGVGITVNSEIPQ"
     gene            2586..3017
                     /locus_tag="VFN58_07280"
     CDS             2586..3017
                     /locus_tag="VFN58_07280"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET8917828.1"
                     /translation="MAGEEEKQEGKGFTVQDRRRFSPETGEAREVSEEAKGFTMPGGG
                     ESVGESQSKTQPSHESLPEINFSTFIISLSTQALMHLGEIANPLSGKNETDILVAKQM
                     IDILAMLQEKTRNNLDTGEETLIEDVLFDLRMKYVEAVKKK"
     gene            3028..4524
                     /locus_tag="VFN58_07285"
     CDS             3028..4524
                     /locus_tag="VFN58_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET8917829.1"
                     /translation="MNLKSIWSGNVGLKGVLLVALVSLVVGLGISGSLDWLSPSRAVN
                     MMGESGNPELRSPGQLPDFVALAKKVRPLVVNISTTQVSEGRGGQQEFGNPFGEDDPF
                     NDFWKRFFGGPVPRGPQRQRSLGSGFIIDADGSILTNNHVVENAQKILVKLSDEQEYE
                     AKVVGRDPKTDIAIIKINTKTGLTAASLGDSDNLEVGEWVMAIGNPFGLDSTVTSGIV
                     SAKGRHIGQGPYDNFIQTDASINPGNSGGPLINLRGEVVGINTAIFSRSGGNIGIGFA
                     IPINLVKELLPQLRGKGKVTRGYLGVLIQKVTPEIADSLGMDRGRGALVANVSKDGPA
                     EKAGVKVGDVIIEFDGKEIKDSGDLPIIVARTPVDRRVRMKVLRDKKELQLTVGVGEL
                     KDEEVVASAPEKGELGMTVQKLTPQIAESLGLERAEGVVVSAVDPGSAADEAGIRRGD
                     VILEVDRKPIRSIDEYKKSLAGIRKGKGVLLLVRRGESTLFLALKPQN"
BASE COUNT         1085 a         1174 c         1376 g         1099 t
ORIGIN      
        1 ggttggtgcc gtccataatg atcacgattc ccaattgatc ggcctgttct cgacaaattt
       61 tgtagagctc gtctttgcag aaaaaacatc ggttcgctgg attctgggta aacgacggat
      121 tggcgagttc gtgcgagtcg aggacgatat gtcggatgcc gaggccggca gcgaactctt
      181 gcgcggctgc cagctccccg ggcggtatcg tcgcggacga tgccgttagg gcgatggcac
      241 gctcgcccaa aaccatgtgg gcaatcgtga gtaagaatgt ggagtccaca ccagccgaga
      301 aagccaccat gactccctcg gttttccgca gtgcgtcctt taattgctct agctttgatt
      361 ccatcgtttt tcgctgaata ggtcggtgct taaataatgc tcgcccttgt cggcgaaaac
      421 agtgacgacg acgtgatctt tccccaaggc ttttgcaact ctcagcgcag cgcaacaagc
      481 ggcaccggac gaaatgccca cgaggagacc ttcctgcgcc gccaaacgtc gagcgcaaat
      541 tgccgcgtcc tcgtcgctca ccggaatgac ttcgtccaga atgtcccggt tgagaaccgc
      601 tgggataaag cctgcgccaa tcccctgaat cttgtgatag ccaggctccc cgccggaaat
      661 taccggcgaa gcagccggct ccacggcgca gacatgaaca tttttcatct tgtctttgag
      721 aacctctccg acgccggtaa tcgtaccgcc ggtgccgact cccgctacga aagcatcaat
      781 tcgcttgaac tgttttagca actcgacggc tgtcgtcttg cggtgcatgt cgggattggc
      841 tggattgttg aactgctgcg gcatgaagta ctcgctatgt tcttgcaaca gctcctcagc
      901 cttgtgaatc gcaccgccca tccccttggt gtcgggagtc agaatcaagt gagcgccgta
      961 cgctgacagt aggctgcggc gctcctcgct catcgtatcc ggcatggtca gaatgagcct
     1021 gtaacctttg accgccgcga tcaatgccaa gcctatccca gtatttccgc tggtcggttc
     1081 aacgatggtg gcaccgggct taagtttccc ctcgctttcg gcgactgcga tcatgttgag
     1141 acaaatacga tccttcaccg acccgccggg gttgaaagac tccatcttag cccatacttc
     1201 cgcgtcgttc tttccaggaa gggttctcag tctcacgacg ggcgtggagc cgatgagatc
     1261 cagaatggat cgggcgccat ttatgcttgc cacagatatg ctcgggtttc cggttgaaga
     1321 gcaattcgaa ccggctcgaa aagaatggta tatttttgac atttcaaagt caacgaaact
     1381 aacttcctcg atcatgtcga tgccggcagc aaagcgagcg cttgtcattt tccctggagc
     1441 cctgggcgat ttcctctgct ttctgcctac gttgcggttt ttgagccaga ataaggaggt
     1501 cgatctcttg gcccgtagtg agttcgccga cttggttcct ccgacagtca aagttggttc
     1561 tctagagcgt tacgagattc gcaggctttt cgtctcggga tctgcgcggg aagaacgatt
     1621 gcgagagttt tttgattctt actcgtctgt cttttcatgg atgggcagcg ggcagcgcac
     1681 ttttgtccaa gaacttgatg cgctgtcccg agggcgagca cgagtttttc cgtttcaacc
     1741 ggcgctaatg cagctgcatc agtctgaata ttacctctcg tgtgttggcg atccagcgct
     1801 cgagactggc aaactagaaa ttcccttgaa gccagaagcg gtggcatgga gcgaacggta
     1861 ttggcagcag cactttttga aaggcaagtt ggttctgttg cttgccccag ggagtggtgc
     1921 gcgggagaaa aactggccgg atgcgtcttt ccgcgtggtc gcggattggt ggcggcgcgc
     1981 ttgcggcagg gtggtcgtta ttcttgggcc tgtcgaagaa gaaaagggag actacaccgc
     2041 tttgtgcgaa ggggctgtgg tggctcgtag tttgaattta ggaaagctgg cggcgctgct
     2101 ggtgcgcagt gacctatacc ttgggaatga tagtggtgtg agtcatttag ccgccgcact
     2161 gggggttgca accgcggtgc tatttggccc ttccaacgtt ggacgatggg caccccgcgg
     2221 caagaatgtc accgtagtga ctcagaacgc cgattgtgca ccatgcacgg tctcgaccat
     2281 gaaaagatgc ccgcatcgac agtgcctgac tacactagag cccgaatatg taatcaaaaa
     2341 actggccggg ttggcggagc agctcacctt gacaaggggg ggggtaggga ttacagtaaa
     2401 ttctgaaatt cctcagtaaa atgagcgagt acagctgttt gccgagacga aatgggcgta
     2461 tcgtttgcgc tctaacgggc gtggaatagc gattaacata catcttcaaa cgattgcctg
     2521 gagagacccg ctagcaaaat agcggaaatc gtttccaggc ttttttctct ctaaggaggg
     2581 tgcctatggc tggagaggaa gaaaaacaag aagggaaagg attcacggtg caagatcgcc
     2641 gccggttttc gccggagacc ggtgaagctc gtgaggtttc tgaagaggcc aaagggttca
     2701 caatgcccgg tggaggtgaa agcgttggcg aatcgcaaag caaaacccag ccgagtcatg
     2761 aatcccttcc tgaaatcaac ttttcgactt ttatcatcag tctcagcacc caggcgctta
     2821 tgcacttggg tgagatcgcg aaccccctga gtggaaaaaa cgaaaccgat attctagtgg
     2881 ctaaacagat gatcgatatt ctcgccatgt tgcaagaaaa gactcgcaat aacttggata
     2941 cgggcgaaga aacgctgata gaggatgttc ttttcgatct gagaatgaaa tatgtggagg
     3001 ctgtgaagaa gaaataggag cgttcaaatg aatttgaaaa gcatctggag tggaaatgtc
     3061 ggcctaaaag gggtcctgtt ggtggctttg gtttcgctgg tggtcggcct gggaatcagc
     3121 ggcagcctcg attggctatc gcccagccga gctgtaaaca tgatgggcga aagtggcaat
     3181 cccgagctgc ggtcgcctgg ccaactgccg gattttgtcg cccttgcgaa gaaggtgaga
     3241 cctttggtcg ttaatatttc cacgactcag gtcagcgaag gacgtggcgg ccagcaggag
     3301 ttcgggaatc cgtttggcga ggatgacccc tttaatgatt tttggaagcg gttctttggt
     3361 gggcccgtgc ctcgaggtcc gcaacggcaa agaagtctag gctccgggtt catcatcgat
     3421 gccgacgggt cgattctgac aaacaaccac gtggtcgaga atgcgcagaa gatcctagtg
     3481 aagctttcag acgagcaaga atatgaggct aaggtcgtgg gacgggatcc aaagaccgat
     3541 atagccatca tcaaaatcaa tactaagacc ggtctgactg ccgcaagtct tggagattcg
     3601 gacaacctcg aggtcggcga atgggtgatg gcgatcggca atccgtttgg cttagacagc
     3661 acagtgacct cgggcattgt gagtgccaag ggtcgtcata tcggccaagg cccctatgac
     3721 aactttatcc agaccgacgc ttcgattaac ccaggaaact ccggcggtcc cctgatcaat
     3781 ctgcgcggcg aagtggttgg tatcaacacc gccatcttca gccgttcggg cggcaatatc
     3841 ggaatcggct ttgccattcc gatcaatctg gtcaaagaac ttctgccgca gctcagaggc
     3901 aagggcaaag ttacgcgcgg ctacctgggt gtgctgatcc agaaggttac cccggaaatc
     3961 gctgacagcc taggcatgga tagaggtcgc ggcgctctgg tcgccaatgt gtcaaaggac
     4021 gggccggcgg aaaaagcggg tgtcaaagtc ggcgacgtca tcatcgaatt cgacggtaag
     4081 gagattaaag actcgggtga cctgcctatc attgttgcac gtactccggt ggatcgccgg
     4141 gtgcgcatga aggtgttgcg cgacaagaag gaactgcaac tgacggtggg cgtcggcgaa
     4201 ctaaaagacg aagaagtggt agcctctgct cccgaaaagg gcgaattggg gatgacggtg
     4261 cagaagttga cgccgcagat tgcagaaagt ttggggctgg aaagagcaga aggtgtcgtt
     4321 gtgtcggccg tagatccagg gagcgcggcc gatgaggcag gcatccgaag aggggacgtt
     4381 attcttgaag tcgatagaaa gcccatacgt agtatcgacg aatacaaaaa gtccctcgcc
     4441 gggattcgca aagggaaagg ggtgcttcta ttagtgcgcc gtggcgagag cacgctattc
     4501 ctagccctca agccgcaaaa ctaattcctg ctgttatttc ccgtctgtga gagggccggg
     4561 taagctgcac ggccctcttt ttgttgcttt tgcgcgggaa ctaggtgaca ataaatttca
     4621 tatttggagt gttaggtttg aaaaggccca aacttcgaat tcttcgtatc atcctcgtct
     4681 cacttctcat cgtcacttcc agcgggctcg tttttggcgg ttggtacctc agac
//
LOCUS       DASSWZ010000302         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_875176, whole genome shotgun sequence.
ACCESSION   DASSWZ010000302 DASSWZ010000000
VERSION     DASSWZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875176"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFN58_07290"
     CDS             <1..571
                     /locus_tag="VFN58_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET8917830.1"
                     /translation="LDEIDKIAGRESTHGPDVSREGVQRDLLPIVEGSTVNTKYGMVK
                     TDHILFIASGAFHTARPSDLIPEFQGRFPIRVELSSLGKEDFVRILTEPKNALIKQYA
                     ALLETEKVHVKFGEDAILEIARIAAEVNERTENIGARRLHTILERLLDDVSFSAPEMP
                     GKDVPIDAQYVRDRLTPILNNEDLSRYIL"
     gene            682..1563
                     /gene="argB"
                     /locus_tag="VFN58_07295"
     CDS             682..1563
                     /gene="argB"
                     /locus_tag="VFN58_07295"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET8917831.1"
                     /translation="MENFISKADVLMEALPYFRRFYGKTFVIKYGGHAMTDEDLKESF
                     AQDIVLLKYVGINPVVVHGGGPQIDEVLDKMGITSRYVRGMRVTDQETLDIVEMVLVG
                     KVNKEIVTLINQHGGMAVGLSGKDGGLILARKMNVTVSANGEPPEIIDIGMVGEIVGI
                     NPLVIDSLDENKFIPVIAPVGCGDKGETYNINADLVAGEVAEALHAEKLILMTDVEAV
                     KDKKGELLSTLRVNQARKMIQDGVVGSGMIPKVECCINALKGGVEKTHIIDGRVKHAV
                     LLEIFTKEGVGTEVVRK"
     gene            1565..>2449
                     /locus_tag="VFN58_07300"
     CDS             1565..>2449
                     /locus_tag="VFN58_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET8917832.1"
                     /translation="MKNRDVAKLTDKYVAQTYARYPIALVRGKGARVWDADGKEYLDF
                     LAGIAVNSLGHCHPAVVRAIQQQSRKLIHVSNLYHILPQSELARELCRHSFAERVFFC
                     NSGAEANEAAIKLARRYGAAHLGGKYEILSAHNSFHGRTLATLTATGQEKVRAGYDPL
                     PSGFRQIPFNDLPAAEEAIDPEKTVGILVEPIQGEGGINVPDQDYLRGLRELCDRRNL
                     VLIFDEVQTGLGRTGKLFGYEHFGMTPDIMTLAKALGGGLPLGAMLAREEVAQSFIPG
                     SHASTFGGNPLVCSVGLAV"
BASE COUNT          552 a          643 c          720 g          534 t
ORIGIN      
        1 ccttgacgag atcgacaaga tcgccggccg ggaatcgacc catgggccgg atgtatcgcg
       61 ggagggggtg cagcgagatc tgttgccgat tgtcgaaggc tccaccgtga acacgaagta
      121 tggcatggtc aagactgatc acatactttt tatcgcctcc ggggcctttc acactgcgag
      181 gccttcggat ctgattcctg agtttcaggg ccggtttccg atccgtgtgg aattgagttc
      241 gttgggtaaa gaagactttg tccgcatctt gacggagccc aagaacgcgc tcatcaagca
      301 gtacgcggct ttgctcgaaa ccgagaaagt tcacgtgaag tttggggaag acgcgatttt
      361 ggagatcgcc cgtatcgccg ctgaggttaa tgaacggacg gagaacatcg gcgcccggcg
      421 actgcatacg atcttggaac gcttactcga cgatgtctca ttttccgctc cggagatgcc
      481 gggcaaggac gtgcccatag acgcccagta tgtccgtgac cggctcaccc ccatcttgaa
      541 caacgaggac ttgtcgcgct atatcctcta atctattaat ctattaatga gttcattcgt
      601 atctgcacaa cggacatcgc ttcgacctcg ggtcgactcc tttcgagcgc atcggccggt
      661 gctttaagag gattgtcctt tatggaaaat ttcatcagta aggctgacgt cttaatggag
      721 gcgcttccct attttcggcg cttctacggc aaaacgtttg tcattaagta tggcggtcat
      781 gccatgaccg atgaagacct gaaggaaagt tttgcccaag acatcgtgct gctcaagtat
      841 gtcggtatta atcccgttgt ggttcacggc ggcggccccc agatcgacga ggtgctggac
      901 aagatgggga tcacgagccg ttatgtgcgc gggatgcggg ttacagacca agagacattg
      961 gatatcgtcg agatggttct cgtgggcaaa gtcaacaagg agattgtcac cttgatcaat
     1021 cagcacggcg gcatggcggt gggtttgagc ggcaaggacg gtggtcttat tctcgcgcgc
     1081 aaaatgaatg ttaccgtttc cgccaatggc gagccgccgg agatcatcga tatcggcatg
     1141 gtgggggaga ttgtaggcat caacccgttg gtgatcgatt ccctggatga aaacaagttc
     1201 attccggtca tcgccccggt gggctgtgga gataaaggcg agacctataa catcaacgcc
     1261 gacttggtag ccggcgaagt ggccgaggcg ctgcacgccg agaagctcat tctcatgacc
     1321 gatgttgaag cggtgaagga caagaagggt gaacttctga gtaccctgag ggtgaatcaa
     1381 gcacgtaaaa tgatccagga cggcgtcgtc ggctcgggca tgatccccaa ggtcgagtgc
     1441 tgtatcaatg ccctgaaagg cggcgtggag aaaacccaca ttatcgacgg cagggtcaag
     1501 cacgcggtgc tcctcgagat ctttacgaaa gaaggagtag gaaccgaggt ggtgaggaag
     1561 tagaatgaag aaccgtgatg ttgccaaact caccgacaaa tacgtcgctc agacctacgc
     1621 acgctatccc atcgctctgg tgcgcggcaa aggcgcccgg gtgtgggacg ctgacggcaa
     1681 agaatatctc gactttttag ccggcatcgc cgtgaacagc ctgggccatt gccacccggc
     1741 cgtcgtccgc gcgatccagc aacaatcacg caaactaata cacgtctcca atctctacca
     1801 tattctgccg cagtcggagc tggcgcggga gctttgtcgc cattcattcg ctgagcgcgt
     1861 ttttttttgt aacagcggcg cagaagccaa tgaagccgcc atcaaattgg cgcggcgcta
     1921 cggcgcggcg catctcggcg gcaaatacga gatcctctcc gcccacaatt cctttcatgg
     1981 gcgcacgctc gccacgttaa cagcgacggg tcaggaaaag gtgcgggcgg ggtacgatcc
     2041 gttgccctcg ggctttcgcc agatcccttt taatgacctg ccggccgctg aggaggccat
     2101 cgatccggaa aaaacggtgg gcattcttgt agaacccatc cagggtgagg gtggcatcaa
     2161 tgtgcctgac caagattatc tccggggtct ccgagagctg tgcgaccgcc gcaacctggt
     2221 tctgatattc gacgaagttc aaactggcct gggacggacc ggaaaactct tcgggtatga
     2281 acactttggc atgacgccgg atatcatgac cctggccaag gctttaggcg gcggattacc
     2341 gctgggcgca atgcttgccc gcgaggaagt cgcgcaaagc tttatccccg gaagccatgc
     2401 ttcgactttt ggcggaaatc cgctcgtttg cagcgtcggc ttggcagtg
//
LOCUS       DASSWZ010000303         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_846769, whole genome shotgun sequence.
ACCESSION   DASSWZ010000303 DASSWZ010000000
VERSION     DASSWZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846769"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFN58_07305"
     CDS             <1..338
                     /locus_tag="VFN58_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET8917833.1"
                     /translation="SGMKAALNGVPSFSVLDGWWIEGHVEGVTGWSIGGPNGRDNDND
                     SETEVQFLYEKLEHIILPLFYRDTDKFTEVMRSAIAINGSFFNTQRMVFQYLRNAYAL
                     GSSSGAPLP"
     gene            complement(378..899)
                     /locus_tag="VFN58_07310"
     CDS             complement(378..899)
                     /locus_tag="VFN58_07310"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET8917834.1"
                     /translation="MARNLKEDFFRLDGAWASFELMSIRRRDDYLKPFRVLRCKIDDQ
                     VDFQAPETLRSATEATVLIEIFGEEELVAASGRGPVQAVDNAMRKILEKHYPQLSEVR
                     LEELDVRLLHHGGIIGDDEDKGAGALVRVLAIFSDGADRWGTVGVAADILQASVECII
                     DGLEWKLRGEHKH"
     gene            1065..1634
                     /locus_tag="VFN58_07315"
     CDS             1065..1634
                     /locus_tag="VFN58_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917835.1"
                     /translation="MDKRVLDGKLHSFFHDVIRQSFRQLGINDSTVTRYMADVLTDFA
                     KSENLYRIRSRKGKRMESVVEILSEQPSAPIDETQLLKERSLRKYLGDYALFMSGIFR
                     SYVEGRGFLHYYIEEGSRSYWTVSELDLSLYRTGFILFQELSKKFEYYSGALDYTRKA
                     YFAAEPGEDPFAGFLRQVEGWMKVNLTEN"
     gene            1686..2717
                     /gene="mltG"
                     /locus_tag="VFN58_07320"
     CDS             1686..2717
                     /gene="mltG"
                     /locus_tag="VFN58_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET8917836.1"
                     /translation="MKSFLITLCLVLVSAVLFAGFVFSYIFFSVSPKSGIVEIRIEQG
                     ESFSTVVRKLRDQKVVTNEKLFSLWARYSGSEKKIHWGLYRFELPLSPSEVLNRMVLG
                     KGIFQRVTIPEGLTVKEVADLLAKMGIVPQEQFLAAAYDSELLSSIGLQDKGIEGYLF
                     PNTYSFIPETPSREIILKMTEQFRKSVEPLAGQQKGDNGLTPHEIVTLASIIEKETGV
                     EAERPLVSAVFHNRLKLKMPLQSDPTVIYGLKDFNGNLTRKDLSAASPYNTYRIPALP
                     PGPICNPGLSSLRAALHPASVPFLYFVSKNDGTHHFSESLESHSQAVKTYQPRNETPP
                     SRRRPAARD"
     gene            2820..>4130
                     /locus_tag="VFN58_07325"
     CDS             2820..>4130
                     /locus_tag="VFN58_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917837.1"
                     /translation="MATQLRNHELWDSLLICSPPLLVCIYLGMYLYRAAWIAPITFLL
                     LSGAVIGLSLLAVVVHTRRLRPSVRSAARLVDEKTEAEDRFLTLATIDVALWPAALVG
                     RLRSEAAALLERIDFRHEFPYRIKRSFYWSFLISLIAATLFHLSMPLMESTRRQAPAY
                     EKLSELIEKMAQRPPLSTLAYDLRTLAMKLQDPNVAEQEKQTLIHKALEQVEKQQKKE
                     KEQESRALLGEASSTLQGLEQQSAGSQQKDTEKGDGGAQSNPSQEGQGEGRQSQGGGD
                     SKGELNAERSKNMQQGQDARENSKEQGKEQSPQSQGDGKDNQPDSGNKSDKGTGKELT
                     GKTPGGSQEKPGRSRSDEIPQGAPPAERFYKPGEQGSEGVKGARYVTVQLPEELAADS
                     KGEGTLSKPSKEPRAYPKIPVSNAPLPAHVPEAPMEKQQLPLEYR"
BASE COUNT          984 a         1138 c         1105 g          903 t
ORIGIN      
        1 ccagtggtat gaaagccgca ttgaacggcg tgccgagctt tagcgttctg gatggttggt
       61 ggatcgaagg ccacgtcgag ggcgtgaccg gttggtccat cggtgggcca aacggacggg
      121 ataacgataa cgactcggag actgaggtcc agttcctata cgagaagctc gagcatataa
      181 ttctcccgct cttttacaga gacaccgaca agtttaccga agtcatgcgg tccgccattg
      241 cgatcaatgg ctcgttcttt aatacgcagc gcatggtgtt tcagtatctg agaaacgctt
      301 atgctctcgg ctctagttcc ggagcaccgc taccgtaact gaaactgacg tgacccaacg
      361 gccacagatg tttcacccta gtgcttgtgt tcgccgcgaa gtttccactc gagaccgtcg
      421 ataatgcact cgacgctggc ctgaaggata tccgcagcca cgcccacagt tccccaccgg
      481 tcggcgccat ctgaaaaaat agccagaact ctaaccaacg cgccggcgcc tttgtcttcg
      541 tcatcaccga tgatcccgcc gtggtggagc aatcgaacgt cgagttcctc caacctgacc
      601 tcggaaagtt gcgggtaatg tttctccagg atttttctca tggcgttgtc aacggcctgt
      661 accggacctc gaccgctggc ggcaacaagt tcctcttccc caaaaatctc gatcaaaacc
      721 gtggcttcgg tggcactgcg cagcgtctcg ggggcctgga agtcaacctg gtcatcgatc
      781 ttacagcgga gcacgcgaaa aggttttaag taatcgtcac gccggcgaat cgacataagc
      841 tcaaacgaag cccatgcgcc atcaagtcgg aaaaaatcct ctttgaggtt ccttgccatg
      901 atttcctccg ggctagcgaa gtgggataac tggagaacgc tgcgctatta tagctttcac
      961 gcagaaaatg gctagagtga agatggccgg cgaattcgcg gcctcgcttg ttgccccaag
     1021 gttaaaatct tcccaaaaaa acccaacttt ggtatcataa accgatggat aaacgcgtat
     1081 tagatggcaa attgcatagc ttctttcacg atgtcattcg ccaaagcttc cgccaattag
     1141 gcatcaacga ctcgaccgta acgcgctaca tggcggacgt tcttaccgat ttcgccaaga
     1201 gcgaaaacct ttatcgcatc cgctcgcgaa agggcaaacg catggagagc gttgttgaaa
     1261 ttctctcgga gcagccatcc gcgccaatcg acgaaactca actcttaaag gaaaggtccc
     1321 tgcgcaaata cctcggggac tatgcgctct tcatgagcgg gattttccgt agttacgtag
     1381 aggggagggg gttcctccat tattacattg aagaaggcag ccgttcgtac tggacggtct
     1441 ccgagctcga tctgtccctt taccgtactg gctttattct ctttcaggaa ctttcaaaga
     1501 agtttgaata ctattccggc gccctggatt acacccgcaa agcctatttt gccgcggagc
     1561 ctggtgagga tccctttgcc ggattcctgc gtcaggtcga gggctggatg aaagtgaatc
     1621 tgacggaaaa ttagagcgtt tgtgcaaccg cggtctttgt ctccacgtta ttttaccttt
     1681 tgggcatgaa atcgttcctg attactcttt gcctcgtgtt ggtttcggct gtactgtttg
     1741 ccgggtttgt tttttcctac attttttttt ctgtttcccc aaagtccggg atcgtcgaaa
     1801 ttcgcatcga acaaggagaa tcattctcga cggtggtccg taagctgcgg gaccaaaagg
     1861 tcgtgaccaa cgaaaaactc ttctcgttgt gggcgcgcta ttccggatca gagaaaaaaa
     1921 ttcattgggg gctctatcgc tttgagttgc cgttgtcgcc ctcagaagtg ctcaaccgta
     1981 tggtgctggg caaggggatc ttccagcgtg tcactattcc cgaggggctg acggtcaaag
     2041 aagtcgcaga tttgctggcc aaaatgggta ttgtcccgca agagcagttc cttgccgccg
     2101 cctacgattc tgagctgctt tcgtctatcg gcctgcaaga caagggcatt gaaggttatc
     2161 tttttccgaa cacttatagc tttattcccg aaaccccgtc ccgagaaatt atcctgaaga
     2221 tgacggagca gtttcgcaag agcgtcgaac cgctggcggg acagcaaaaa ggggataacg
     2281 gtctgacgcc gcacgagatc gtgaccctgg cttcaattat tgagaaagaa accggcgtcg
     2341 aagccgagag gccattggtc tcggccgtat ttcacaaccg gctcaagctg aaaatgccgc
     2401 tgcaaagcga tcccacggtt atctacggtc tcaaagactt taacggtaac ttaacccgca
     2461 aagatttgtc tgccgcgagc ccctacaaca cctatcgcat tcccgctctc cccccggggc
     2521 cgatttgtaa ccccggtctc tcatctctac gagccgcact ccatcccgct tcggtgccgt
     2581 ttctctattt cgtgtcgaaa aacgacggca cccaccattt ctcggaaagc ctggagtccc
     2641 acagccaggc agtcaaaacc tatcaaccgc gcaacgaaac acctccatcg cgtcgcaggc
     2701 ccgctgcgcg cgactaacga tgcatttatg gcgtctcact aaacatgcta tagttagccg
     2761 gcatgaggac gtccacggat gcacagggtc gagagtcgcc tgagcagcgg gtcggtcggt
     2821 tggcgacgca gttacggaat cacgagctgt gggattctct cctgatttgc tcgccgccgt
     2881 tgctggtttg catttacctc ggcatgtatc tctatcgtgc cgcgtggatc gcaccgataa
     2941 cgtttctttt actcagcggc gcggtgatcg gattaagttt gcttgcggtg gtggttcaca
     3001 cccgtcgttt gcgcccttcg gtccgttccg ctgctcgact ggtggacgaa aagacggaag
     3061 cggaagaccg cttcctcacg ctggcaacga tcgacgtggc cctctggccc gccgccttgg
     3121 tggggcgtct acgcagcgag gctgcggcgc tcctggagcg gattgatttc cgtcatgagt
     3181 ttccctatcg catcaaacga tccttttact ggtcgttctt gatttctctc atcgcggcca
     3241 ccctgtttca tctctcgatg ccgctcatgg agtcaactcg tcgccaggct ccggcctacg
     3301 agaagcttag cgagcttatt gaaaaaatgg cccagaggcc gcctctgtca accttggcct
     3361 atgatctgcg cacccttgca atgaaactcc aggacccaaa cgtagcggag caggagaagc
     3421 agacgctgat tcacaaagcc ctggaacagg tggagaagca gcaaaagaaa gaaaaagagc
     3481 aagaaagccg ggctcttctc ggtgaagctt cgagcacgct tcagggtctg gaacagcagt
     3541 ccgctggaag tcaacaaaaa gacacggaaa aaggcgatgg cggtgcgcaa agcaatccct
     3601 ctcaggaagg gcaaggcgag ggaagacaaa gtcaaggcgg cggtgatagc aaaggcgagc
     3661 tgaacgctga acgaagtaaa aatatgcaac agggccaaga cgcccgggaa aattcgaagg
     3721 agcaaggcaa agaacaaagc ccgcagagcc agggtgatgg caaggacaat caaccggact
     3781 ccggcaacaa gtccgacaaa ggcaccggta aagagctcac cgggaaaacc ccaggcggct
     3841 cccaggaaaa acccggcagg agccggtccg acgaaatccc ccagggcgcg cctccggcgg
     3901 agcggtttta caaacccgga gagcaaggta gcgagggagt caaaggggcc cgttatgtaa
     3961 ccgtgcaatt gccggaggaa ctggccgcgg attcgaaggg cgaagggaca ctcagtaagc
     4021 cgtcaaaaga acccagggcc tatccgaaaa taccggtgag taatgctccg ttgcccgccc
     4081 acgtgccgga ggccccgatg gagaaacaac aactgccctt ggagtaccgc
//
LOCUS       DASSWZ010000304         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1002469, whole genome shotgun sequence.
ACCESSION   DASSWZ010000304 DASSWZ010000000
VERSION     DASSWZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002469"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFN58_07330"
     CDS             complement(<1..392)
                     /locus_tag="VFN58_07330"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET8917838.1"
                     /translation="MAKTTTKRPLCIVIAGPNGAGKTTFAREFLLKDTRVIHFVNADV
                     IASGLSPLRPEMVALAAGRIFLRELDRLAKARADFAFESTLSGLVYLNRLKRWKVAGY
                     RIEIVYLRLPSPRLALRRIAARVKQGGH"
     gene            complement(448..717)
                     /locus_tag="VFN58_07335"
     CDS             complement(448..717)
                     /locus_tag="VFN58_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET8917839.1"
                     /translation="MKEDSNAKDEKMRAENGRIKGDLKERTKEFALNIIALYSQLPKR
                     REAQVLGDQLLRSGTSVGARSSELDMRKVLLSSSRQTLVRPLLDR"
     gene            1691..2935
                     /locus_tag="VFN58_07340"
     CDS             1691..2935
                     /locus_tag="VFN58_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HET8917840.1"
                     /translation="MARSIVEISGHIIDSLILPKILDLIVNLGAEFEILDIQIGHRRA
                     DRSHARVQVEASSEELLAEVLAKIREHGALPVEGSEEPARLDAAPADGIFPEAFYATT
                     NLPTQVRVNGRWIEVETPEMDCAIRVDGSAMRAETVPVHKVRKGDSIVVGHQGIKIVP
                     LERSVPHELFEFMSSAVSTEQPKGLLIREIAETMKNVRQKNGKILVVAGPGLVHTGAA
                     PYLVDLIERDYVQVLFAGNGLAVHDIETALYGTSLGVYLDKKIRASEGHEHHLWAINT
                     IRKAGGIRQAVEQGIVQRGIFYSCVRKGVDFVLAGSIRDDGPLPDVITDSLKAQDLMR
                     EKVRGVSLALMMATTLHAIATGNLLPATVKTVCVDINPAVVTKLTDRGTFQALGLVTD
                     VEPFLRELCQCLNQEPLQAHEP"
     gene            2959..3750
                     /locus_tag="VFN58_07345"
     CDS             2959..3750
                     /locus_tag="VFN58_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HET8917841.1"
                     /translation="MQLLMCAPSYYGIEYEINPWMSRARQSDPQRAQQQWHSLRRLLE
                     EQLSLDIRLVDAQPELPDMVFTANAGLTWKNKFIVSNFRYEVRRREAPHFEAWFRNLG
                     FEIVHLPREYCFEGEGDLLKCGESWFAGYHIRSDINAHQKVAEIIEQEILSFELTNEW
                     FYHLDTCFCPLSDEQVLFYPPAFDSYGLEVLEKHIPDLIPVAPEEAARFACNAIVADR
                     DIVMNTGCPGVREKLEALGFRVFETPLDEFIKAGGSAKCLVLYIH"
BASE COUNT          956 a         1129 c         1116 g         1078 t
ORIGIN      
        1 ttatgcccac cctgctttac tcgggccgcg atgcggcgga gtgcaagtcg cggggaaggc
       61 aaacgtaggt aaacaatttc aatgcgataa ccagcgacct tccagcgctt taaccggtta
      121 agatagacga gcccgctcaa cgtgctctcg aacgcgaagt ccgcgcgggc tttggcaaga
      181 cggtctaact ctctcaggaa gatacgccct gctgccaggg cgaccatttc cggccgcagc
      241 ggtgaaagac cgctagcgat cacgtcggca tttacaaaat ggatcacgcg ggtatcttta
      301 agcagaaatt ctcgagcaaa ggttgtttta ccagcgccgt taggaccggc aatgacgata
      361 cagagaggcc gctttgttgt tgtcttggcc atctaaaatg gattattcct gtcggcacgt
      421 tgatttgaca aaaaggtttc gcggatttta acggtccagc agcggtctga caagtgtttg
      481 acgagatgaa ctcaaaagaa ctttcctcat gtcgagttcg ctacttcgtg ctcccacgga
      541 ggtacccgat ctcaatagct ggtcaccgag gacttgggcc tctcttcttt tcggtaattg
      601 cgaatagagt gcgatgatat tcaacgcgaa ctccttcgta cgctctttga ggtcgccctt
      661 gattcttccg ttttcagctc tcattttttc atctttggcg ttggaatctt ctttcatcct
      721 tccgttctaa cttttcatgg tttccccttt ttcagctttc cgttttcatc gtttcgttct
      781 tcccgtcgta cggtgccgac tcccccgaac ttgctgtcgt cgcggagcca gtatttattc
      841 tctgacggcg ttggtggcta gactatagag ctctggatct ttatttgact tcgagagccc
      901 gctccagcgt aatccgtttc tgttcacaaa gtccgaccaa gtaggctaga gagttgacct
      961 caatccccgc agcctgtgcc gcctcataag gtctccagtc atcgatgagc aataggaact
     1021 ttcgctccaa agcgaggtta atagcggctc tctccccatc tatttaatct tcgccgatct
     1081 tggcgcagcg cgtttgatgc ttttgtccgc tagtaatgcc ttgagtcggc ggctcgttgg
     1141 gttatcgcgc cctaactcca cactccgaaa aaacgaaagg gcatctttcc cgggaaagat
     1201 gcccttggtt gcgctttttg cgcgtataac tagttttatc ttttcttgtt gttcccgcct
     1261 gtcagtggct tagcgtcact tgaccgggac cgcaatcacc tctgccacta cggagtctca
     1321 tgccagctac gaccttcctt cgttcgtcgc ggtagcgacc gtttgcctac aggcaatttg
     1381 cttgaggtct agtggaaata gctcctaagc gtgagacaac tcgtgggccc cgaaaagcga
     1441 cggcccacag acagcgggct tttttcgtca ggtggagtac tgtcttgcat agcaaagacc
     1501 tcctttcacc ttggggctcg ctaacctatg cgatccactg gcccccacgc ctagcggcat
     1561 tgaatggatc taaatgtgtt tctagtcctg tttcgcgtgt ttgtcaacga gggaaaattt
     1621 tttggcttca cctttagaac gatgaaaaac gatacattgc acgattgact aggccgggat
     1681 aaaattacgc atggctcgga gcatcgtcga gatttccggt catatcattg attctcttat
     1741 cctacccaag atcttagacc tcatcgtcaa cctcggcgct gagtttgaaa ttctcgatat
     1801 tcagatcggt catcggcgcg ccgaccgcag tcatgcgcgg gtccaggttg aagcgtccag
     1861 cgaggagctg ctcgcagagg tcttagccaa gatcagagag cacggcgctt tgcctgttga
     1921 aggaagcgag gaacccgccc gactcgatgc ggcgccggca gacggcattt ttcccgaggc
     1981 attttacgct acgaccaatc tgccgactca ggtccgggtg aatgggcgct ggattgaagt
     2041 cgaaacgccc gagatggatt gcgcgatacg ggtggacggg agcgcgatga gggctgagac
     2101 cgttcccgtg cacaaagtca gaaagggcga ttctattgtc gtcggccatc aggggatcaa
     2161 gatcgttcca ctcgagcgaa gcgtgccgca cgagctgttt gagttcatgt ccagcgcggt
     2221 ttccaccgag caacccaagg ggcttttgat tcgagagatc gccgagacga tgaaaaacgt
     2281 acggcagaaa aatggcaaga tcttggtcgt ggctggtccc ggtttggtac acacgggcgc
     2341 cgccccttat ctcgtcgatc tgatcgagcg tgattacgtg caggttctct tcgccggaaa
     2401 cggcctggcg gtccacgata ttgaaacagc gctttacggc acctctctag gagtttattt
     2461 agacaaaaaa atccgcgcca gcgaaggcca tgaacatcat ctctgggcga tcaacacgat
     2521 tagaaaagcc ggcggcatca ggcaagcggt cgaacagggg atcgttcagc gtggaatatt
     2581 ttacagttgc gttagaaaag gcgtggattt tgttctcgct ggatcgatcc gcgacgacgg
     2641 tccgctgcct gacgtgatta cggatagctt gaaagctcag gatctgatgc gtgagaaagt
     2701 acgtggcgta agtttggctt tgatgatggc taccacactg catgcgatcg cgaccggcaa
     2761 tctcttaccc gcgacggtga agacggtttg tgtggatatt aaccccgccg tcgtcaccaa
     2821 gctgaccgat cgcgggactt tccaggcgct cggcttggtc accgacgtcg agcctttttt
     2881 gcgggagctt tgccaatgcc tgaatcagga accgctccag gcccatgagc cgtaagaccg
     2941 ctgggtaacg aggtcgttat gcaactcttg atgtgcgctc cttcgtatta cggcatcgaa
     3001 tacgaaatca acccttggat gagccgggcg cgccagagcg atccccaacg cgcgcagcaa
     3061 caatggcact ctttgcgccg ccttctcgaa gaacaattgt cgcttgacat ccgcctggtc
     3121 gatgcccagc cagagcttcc ggacatggtc ttcacggcaa acgcgggcct gacgtggaaa
     3181 aacaaattca tcgtgagcaa cttccgatat gaggtcagac gccgagaagc gccgcacttt
     3241 gaagcgtggt ttcgcaatct tggatttgaa atcgttcacc tgccgcggga gtattgcttc
     3301 gagggcgagg gcgatctctt aaagtgcggc gaatcttggt ttgcgggata ccacatccgt
     3361 tcggatatca acgcacatca aaaagtcgcc gagattatcg agcaagaaat tctctctttc
     3421 gaattgacca acgaatggtt ttaccatctc gatacctgtt tttgtccgct ttcggatgag
     3481 caagtcctct tctatccccc cgcctttgat tcctacggtc tcgaagtttt ggaaaaacat
     3541 atcccggatc ttattccagt tgcgcctgag gaggctgccc gattcgcctg caacgccatc
     3601 gtcgcggaca gagatatcgt catgaatacc ggctgcccag gggttcgcga gaagctcgag
     3661 gcgttgggct ttagggtttt cgagacaccg ctcgatgagt ttatcaaagc cggaggaagc
     3721 gccaagtgcc tggtgctata tattcactga gttctgcaat gcgggggtcg gcgggacacg
     3781 agcgttaata actcgcatca ctgtccgccc cgctatgcgt tattaaagag aagacattca
     3841 ttgagattcc cacacgcgat tgttgttccg cgtcccgctt aaggtcgtgg gagcaagagt
     3901 ttctcttggt cagcgagtag cttcgtgctc acggggtaac gatcttttaa cgcaaaacca
     3961 gcttgctacc tcttgcgcgc cattacagct ctccaaaatg caataacttt cagccttaac
     4021 ctaaccacac tccggaagga ttactgtcta atagtgaacg gttttgaaac gaattatcgc
     4081 gaccaaaaga cctgatcgtg cttcctatta aaggatgaag gataagggat aaaggataaa
     4141 aaaggagatc cgtgttcctg gctcgtgttc gtgcttcgcc agtggtaact ttcacgtttt
     4201 gataattctc aaaacgtcaa aaatgaccca cctaatcaac cgcgcacggc cagatgttgc
     4261 ctatggaata gcgatttgc
//
LOCUS       DASSWZ010000305         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1229245, whole genome shotgun sequence.
ACCESSION   DASSWZ010000305 DASSWZ010000000
VERSION     DASSWZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229245"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFN58_07350"
     CDS             complement(<1..288)
                     /locus_tag="VFN58_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917842.1"
                     /translation="MYQVKNRCKRFKALLQTLIVVLYFALALAACSTVRPPVSAMPAL
                     TDDLDRESLHTALRRSVSYLEKLPPDRIVGEQPRRFTAGEVLDSLLAFEKSL"
     gene            300..668
                     /locus_tag="VFN58_07355"
     CDS             300..668
                     /locus_tag="VFN58_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET8917843.1"
                     /translation="MDKNEIRKEVARVLDKLPRQFRKQLHNVEIVVEERPGEELFLDA
                     GLDPEHDTLYGLYQGIPLPDRSSLYPPILPDKITIFAEPLLEDFPDPDELREQIRTTL
                     LHEIAHYFGFDDDEIDKLGY"
     gene            complement(683..1342)
                     /locus_tag="VFN58_07360"
     CDS             complement(683..1342)
                     /locus_tag="VFN58_07360"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET8917844.1"
                     /translation="MALSPKDILSALQWDFLSFFFQGAPPFFLTGGTALSAFYLQHRY
                     SDDLDLFTLDNGAFDRVPLYVADTGTRLNASVVSLQTAPQFHRYRLSRNQESVIVDFV
                     KEVVPQISQAKNTFEGIVVDTLDDIAANKVCTVVSRAEIKDYIDLYFLARAGYPLERF
                     IQPAQQKDAGVSQAMVAYLLSDFRLSKVPDFMIAAISLEEVQEYFQSLARKLAVESFP
                     Q"
     gene            complement(1326..1601)
                     /locus_tag="VFN58_07365"
     CDS             complement(1326..1601)
                     /locus_tag="VFN58_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917845.1"
                     /translation="MTQELTDRLSFLWDEPLTRGDLKKLLDGGNEEERLYYAAKILRE
                     ARFEEVWDYLSPMFLASHWEGLRRRLGRKKRFWEFFYATWREHGLIT"
     gene            complement(1692..>2060)
                     /locus_tag="VFN58_07370"
     CDS             complement(1692..>2060)
                     /locus_tag="VFN58_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917846.1"
                     /translation="PKVLKRCDVSLKLGKNTIEAMDEGMVRLHGNVGYIAGQPEERSR
                     IPNPVVDNYRGDHFKYFMDFKAGKVPGRTNDKQITFFINAGTQGLQFAACAGKIYQLA
                     KEKGVGRELPTEWFLQDIRD"
BASE COUNT          525 a          558 c          498 g          479 t
ORIGIN      
        1 gagtgatttt tcaaaagcca gaagcgagtc caaaacttcg ccagcggtga agcgccgggg
       61 ttgctccccg acaatccgat ccggcggcag tttctccagg taagagacgc tgcggcgtag
      121 cgccgtgtga agcgattccc gatcaaggtc gtcggtcaac gccggcatag ccgaaaccgg
      181 cggccgtacg gtgctgcatg cagccaaggc aagagcaaag tagaggacca cgattaatgt
      241 ttgaagcagc gccttaaatc gcttgcaccg attctttact tgatacaaac tcttcaccgg
      301 tggataagaa cgaaatcaga aaagaagtcg ctcgcgttct cgacaagctc cccaggcagt
      361 ttcgtaagca gctgcacaac gttgaaatag tggtcgagga gcggcccggg gaagagcttt
      421 ttctcgatgc cggactcgac cccgagcacg acacgcttta cgggctctat caaggcatcc
      481 ctctgcccga tcgctccagc ctttacccgc ccattctacc agacaaaatt acaattttcg
      541 cggagcctct gctcgaggat tttcccgatc ccgacgagtt gcgcgagcaa attcggacca
      601 cgttgctgca tgagatcgcc cactacttcg ggtttgacga tgatgagatc gacaagctgg
      661 ggtactaaac agacaagcct ctctactgag gaaaagactc tacggcaagc ttcctagcaa
      721 gagattgaaa atattcctga acctcctcaa gcgaaatagc ggcaatcatg aagtcgggga
      781 ccttagaaag cctgaaatcg gacaagagat aagccaccat cgcttgtgaa acgcctgcgt
      841 ctttctgctg ggctggctgg ataaaccttt ccaatggata tccggcgcgt gcgaggaaat
      901 agaggtcgat ataatctttg atctctgccc ggctaactac cgtgcaaacc ttattcgcag
      961 caatatcatc caatgtatcc accacgattc cctcaaatgt attttttgct tgtgatattt
     1021 gtggaactac ctctttaacg aagtctacaa tgaccgattc ctggttccgg gaaagccgat
     1081 aacgatgaaa ctgaggcgcg gtctggagcg aaacaaccga ggcattcaac cttgttccgg
     1141 tatcggccac atagagaggc acgcggtcga aggcaccatt gtccaacgtg aacagatcca
     1201 ggtcgtcgga atagcggtgt tgaagataaa aggcgcttag ggctgtaccc ccggttaaga
     1261 aaaagggagg agcgccttga aaaaaaaatg acaaaaaatc ccactgcaag gcggagagga
     1321 tatctttagg tgataaggcc atgctcacgc cacgtagcgt aaaagaactc ccagaaccgt
     1381 ttcttgcgtc ccaatctccg gcgaagtcct tcccaatgag aggccagaaa catcggagag
     1441 agataatccc agacttcttc aaatcgtgct tctctcaaaa tcttggccgc gtaataaaga
     1501 cgctcttcct catttccacc gtccaaaagc tttttgagat cgcctcgagt aagcggctca
     1561 tcccacaaga aactgagtct gtctgtgagt tcctgagtca tgtgttattt taccactatt
     1621 cccgccaggt cttaaactcg ccatcaagcg cttcggggaa ctattgagtc tccttctcga
     1681 tctccgtttt gtcaatccct aatatcctgt agaaaccact ctgtcggcag ctcgcggccc
     1741 actccctttt ctttggccaa ttgataaatc ttccccgcgc atgcggcaaa ctgaagtcct
     1801 tgcgtaccgg cattgatgaa aaaagtaatc tgcttgtcat tggttcgccc cgggaccttg
     1861 ccggctttga agtccatgaa atatttaaaa tggtcgcctc ggtagttgtc gaccacggga
     1921 ttgggaatcc ggctcctttc ctccggctgg ccggcgatat aaccgacgtt gccgtgcagc
     1981 cgcaccattc cctcgtccat cgcttctatc gtattcttgc cgagtttcaa ggaaacatca
     2041 caacgcttga ggactttcgg
//
LOCUS       DASSWZ010000306         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1285924, whole genome shotgun sequence.
ACCESSION   DASSWZ010000306 DASSWZ010000000
VERSION     DASSWZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285924"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VFN58_07375"
     CDS             <1..1300
                     /locus_tag="VFN58_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET8917847.1"
                     /translation="AILIPVLSPAADSPAPAQLMLSQTAAPQPGNVLTLDEALQIALE
                     NHPSIKVSKERIDTQQAVVGQQMAAYYPQINMTNGLQSGTQAGSTTGVADRSFETYLI
                     RTGVNMILYNFGKREGNVQSARDTLDATNFNYKTTVDGVIFFVKQSYFTYLGLRAIVK
                     VREETVKSRQLLARQAQGFYDVGTRARIDVARAESNLYLAEADLITAQNAVKVAWATF
                     KNTLGVRVLPERPLVEDVTMTPVQYSLDQAKELAFASRPELKSFDARRRAQDQLIAVA
                     RRGHLPDLIFDANYRRSNTSIVENTFPLLGGWQLGLNLLIPIFDGFRTTNRVQETLHT
                     YYQIKSQEELQWQQVALDVEQAYLRLVELQERIKANEAAASAAKENLDLANGRYEVGV
                     GSIIEATDAQNLYTDAQTTYIRTLYDYKIAEAALVRAIGQ"
     gene            1375..2661
                     /locus_tag="VFN58_07380"
     CDS             1375..2661
                     /locus_tag="VFN58_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET8917848.1"
                     /translation="MRRSLWIVILVLVLGGFAGIQQYKSWSLNKPQAKSGSSPEGASE
                     GRSVDRAQQQGRSGGGRSREAVPVLIATATQKTMPLQIRAVGNVEAYSTVSVKSQVTG
                     VLTQAHFKEGQDVKKGQLLFTIDPRPFEAALKQAEANLARDVAQLQNSREQARRYAEL
                     VKKEYVSKEQYDQIRANADAAEAVVQADQAAVENAKVQLSYCYIFSPIDGQVGTLLVN
                     EGNLVRVNDGTPLVVINQITPINVTFSVPEQNLADVRRHMAAGSLKVAAQFQSDEGRP
                     EQGSLVFVDNAVDRTTGTIKLKAEFKNGDRRLWPGQFINVALTLTTQSDAVVVPAEAI
                     QVGPEGQHVFVVKPDNSVEVRPVVVARTDEGEAVIAQGLQSGEQIVREGQFLLSAGSK
                     VEIKDTNQERKASETKDRKKDRAGKTKAKAQEGGES"
     gene            2658..>3668
                     /locus_tag="VFN58_07385"
     CDS             2658..>3668
                     /locus_tag="VFN58_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET8917849.1"
                     /translation="MNISGLFIRRPVTTTLVMLGILMFGIMAYKSLPVSDLPTVDFPT
                     ITVSATLPGASAENMAASVATPLEKNFSTIAGLDSMSSTSTLGRTQITLQFILSRNID
                     AAAQDVQSMIARASRDLPRDMPTPPSFRKVNPANNSILLLSLTSETLPLSAVDEFAQT
                     ILAQRISMISGVAQVDVIGSQKYAVRIQLDPNQLAARQIGIDDVVLAIQRGNVDLPVG
                     TLNAPTRAYTLVSDAQLTNAAAYGPMVITYRNGAPVRVQDIGKAIDSVENNKVGSWFK
                     DKPAVILGVQRQPGANVVEVVDAIKQVLPTLRAQIPESIKLDVFFDRTQSIRESAHDV
                     QLT"
BASE COUNT          904 a          943 c         1052 g          769 t
ORIGIN      
        1 ggcgatactc attccggtgc tgagcccggc ggcggacagt cccgcgccgg cgcagctcat
       61 gctgtcgcaa acagcagccc cgcagccggg gaatgtcttg accttggacg aagctcttca
      121 gatcgccttg gagaatcatc ccagcatcaa ggtttcaaag gaacggatcg acacgcagca
      181 ggcggttgta ggacaacaga tggctgccta ttatccgcag attaatatga ccaatgggct
      241 tcagagcggt acccaagccg gcagcaccac cggggtagcg gatcgctcat ttgaaaccta
      301 ccttatacga accggcgtga acatgatcct ctataatttc ggcaaacgcg agggaaacgt
      361 acaatccgct cgggatactt tggatgcgac gaattttaac tataagacca ccgtggatgg
      421 tgtcattttt ttcgtcaaac agtcttattt tacgtatctg gggctcagag ctatagtgaa
      481 agtaagagaa gaaactgtta aaagtcggca gttgttagcg cgacaagcgc agggtttcta
      541 tgacgtcgga accagggcaa ggattgacgt cgccagggct gagtccaact tatacctggc
      601 agaagccgac ctgattaccg cccagaatgc cgtcaaggtt gcctgggcga cctttaaaaa
      661 cacgctgggc gtgagggtac tgcctgagcg gcccttggtt gaggatgtca ccatgacacc
      721 ggtccagtac agtctcgatc aagcaaagga attggcgttt gcgtctcggc cggaactgaa
      781 aagctttgat gctcgaagaa gggcccagga tcaactcatt gccgtcgctc gccgtgggca
      841 tcttccggat cttatcttcg acgcgaatta ccgacggagc aataccagca ttgtcgaaaa
      901 tacattccct ctgttgggag ggtggcaact cgggttgaat cttttgatac cgattttcga
      961 tggatttcgc actaccaaca gagttcaaga gacgttacat acctattatc aaatcaagtc
     1021 gcaagaagag ctgcagtggc agcaagttgc tttggatgtg gaacaagcct accttaggtt
     1081 ggtggaattg caggaaagga taaaggccaa tgaggccgcg gccagcgccg ctaaagagaa
     1141 tctggatctg gccaacggaa gatatgaggt gggggtaggt tcgatcattg aagcaaccga
     1201 tgcccagaat ctttacacgg acgcccagac gacgtatatt cggacgcttt atgattacaa
     1261 gatcgctgaa gccgcactgg tcagggctat aggacaataa cgggtacggt gctatgatct
     1321 aaacggcgta gagacaacga tggttcaaca gcccatgggt tagggagaat aggtatgcgg
     1381 cgttcactct ggattgtcat actggtgctg gttctgggtg gcttcgcggg aattcaacag
     1441 tacaagtctt ggtcgctcaa caaacctcaa gctaagtctg gaagtagccc ggaaggagca
     1501 tctgaaggtc gatccgttga ccgggcacag cagcagggcc gctcgggagg tggacgatct
     1561 cgcgaggccg tacctgtcct cattgctact gccacgcaga aaacgatgcc gctccagatt
     1621 cgggcggtcg gtaacgtcga ggcttactcg accgtttccg tgaagagcca ggttacgggc
     1681 gtgctgacgc aggcccactt caaagaaggg caagatgtca agaaaggaca gttgcttttt
     1741 accatcgatc cccgtccgtt tgaggctgca cttaagcagg ctgaggcaaa ccttgcacga
     1801 gacgtagcgc agcttcagaa ttcgcgtgaa caggcccgcc gctatgccga gctcgtaaag
     1861 aaggaatacg tgtcgaagga gcaatatgac cagatccgcg ctaacgccga tgctgccgag
     1921 gctgtggtgc aggcggacca agctgcggta gaaaatgcta aagttcagtt aagctactgc
     1981 tatattttct ctccgataga cgggcaggtc ggaaccttgc tggtgaatga aggcaacttg
     2041 gtgcgggtta acgatgggac gcccttagtg gttatcaatc agatcactcc catcaacgtg
     2101 actttttctg tgcctgagca gaatttggcg gacgtcagac gccatatggc tgcgggcagt
     2161 ctcaaggttg ccgcgcagtt ccagtccgac gagggacgcc cggagcaagg aagtctcgtc
     2221 ttcgttgaca atgcggttga ccgcaccacc ggaactatca aactaaaggc ggaattcaag
     2281 aacggcgaca gacgcttgtg gccgggccag ttcatcaacg tcgcgttaac tctcactacc
     2341 caatcggatg cggtggttgt acctgctgaa gcgatccagg tcgggccgga gggacaacat
     2401 gtttttgtcg tgaaaccgga taacagtgtg gaggtgcggc cggtggtggt ggcacggacc
     2461 gacgaaggcg aagcggtgat tgcccagggg cttcagtcgg gagagcagat cgtgcgagag
     2521 ggccaatttt tactgagtgc tggttccaaa gtggaaatta aggataccaa ccaggagaga
     2581 aaagcgagcg agacaaaaga tcggaaaaaa gaccgagcgg ggaaaacaaa agcaaaggcg
     2641 caagaagggg gcgagtcatg aacatctccg ggctttttat tcgcagaccc gttaccacaa
     2701 cgctggtgat gctcgggatt cttatgttcg gcatcatggc ttataagtcg cttcccgtaa
     2761 gcgacttgcc aaccgtcgac tttccgacca ttacagtcag tgccaccttg cccggcgcca
     2821 gcgcggaaaa tatggcggcc tctgtggcta cgcccctcga gaaaaacttt tcgactatag
     2881 cggggctcga ttccatgagt tcaaccagca ccttggggcg gactcaaatc acgctgcagt
     2941 tcatcttgag ccgaaacatc gatgcggcgg ctcaagacgt tcaatcgatg atcgcgcgcg
     3001 cctcgaggga tctgccgcgg gatatgccaa cgccgccctc ttttcggaaa gtcaatccgg
     3061 caaataattc catcttgctc ctgtccttga cgtccgaaac gctcccctta tcggcagtgg
     3121 acgaatttgc gcagacgatc ctcgcccagc gcatctccat gatcagcggc gttgcccagg
     3181 tggatgtcat cggctcgcaa aaatacgcgg tccgaattca actggatccc aaccagttgg
     3241 cggcccggca gatcggcatc gacgatgtgg tccttgcaat ccagcgcggc aacgtggatc
     3301 tgcctgtagg gacattaaac gcgcccaccc gggcttacac cttggtatcc gatgcccagc
     3361 tcaccaacgc ggcggcttat ggaccgatgg tcatcaccta tcgcaatggg gcaccggtgc
     3421 gcgttcagga tatcggcaaa gccatcgaca gcgtcgagaa caataaagta ggcagttggt
     3481 ttaaagacaa acccgccgtc atcttaggag tgcaacgaca accaggggcg aatgtcgtcg
     3541 aagtggtgga cgccatcaag caggttctgc cgactcttcg cgcgcagatt ccggaatcga
     3601 tcaagctcga tgtgtttttc gatcggaccc aatcgattcg agaatcggcg cacgacgtgc
     3661 aattgacc
//
LOCUS       DASSWZ010000307         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_663031, whole genome shotgun sequence.
ACCESSION   DASSWZ010000307 DASSWZ010000000
VERSION     DASSWZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663031"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VFN58_07390"
     CDS             <1..1275
                     /locus_tag="VFN58_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET8917850.1"
                     /translation="VASDPMRLLERHLRVDGQILSAGERLYDLAQHSNLYVIGAGKAT
                     ARMALAVEALVGERIAGGIVIVKRGHTVPLRKVEVVEAGHPIPDQAGVNATETIIGLL
                     RRTQKTDLILCLVSGGASALLSCPVVGLSLQDKQQTTQALLNCGARIQEVNAIRKHIS
                     RVKGGRLAELAHPSTVLSLILSDVIDDSMDNIGSGPTAPDSSTFADCLSIIEGYGVGE
                     MIPVAVRSLLKKGAAGEIVDTPKTGDPVFQKVQNLLVGNNQSALVAAKEKAEALGYNT
                     LVLSRSIEGEARKVAMDHVVLARDVLSGSRRIQRPACIISGGETTVAIQGNGLGGRNQ
                     EFALAAAIEIDGLGEVIVLSGGTDGTDGPTDAAGGIVDGTTVQRGRDKGLDAREYLKR
                     NDSYPFLKTVGDLLVTGPTLTNVMDLRLILIV"
     gene            1456..1671
                     /locus_tag="VFN58_07395"
     CDS             1456..1671
                     /locus_tag="VFN58_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917851.1"
                     /translation="MIDVSLRGVRCRAAQEILDLDGTIGAATEGTIVYELEGEGGQLV
                     HVQWDNGTSSLVSSEEIEITDRAVIWQ"
     gene            complement(1697..>3185)
                     /locus_tag="VFN58_07400"
     CDS             complement(1697..>3185)
                     /locus_tag="VFN58_07400"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HET8917852.1"
                     /translation="EKTSWSCFHGIDRWWSNCGCNSGGRPGWNQEWRTPLRGALDTLR
                     DSLALPFEKQGRELLKDPWAARNDYLSVIHDRSTENVEGFFARHALRPLSAAEITTAL
                     KLLELQRHGMLMYASCGWFFDELSGIETVQAMLFAGRAVQLAQDLFGDSLESQFMERL
                     ASAQSNLPEYGDGRRIYEKFVRPAMVDWEQIGAHYAVSSLFERYPERTNICCYRTERR
                     DYQVFEAGLTKLAMGQVKLTSEITWESIVLNFGVLHMGDHHVNCGVQESLGNEPDPSL
                     AREAVEAFRRADFAEVIRFMDRRFGESNYSLWSLFRDEQRKVLEQILTASLGEAETLY
                     RQIYERRAPMMRFLTNLHIPLPKVFSAAAELVLNGYLRQALEQEEVDAERVNALLEAA
                     KLEGVPLDTATLEFAYRHKLERLTERLIVSPTVDLLQQLDSAASLIRALPFRVDLWKI
                     QNSYYRLLENIYPNMQRQKEQGDLAAQAWLNAFKALGRKLAVKVS"
BASE COUNT          726 a          847 c          926 g          686 t
ORIGIN      
        1 gtagcttctg atccgatgcg cctattagaa cgacacctgc gagtcgacgg gcaaatttta
       61 tcggccggcg aacgtcttta cgacttggca cagcacagca atctttacgt gatcggtgcg
      121 ggaaaggcga cagcgaggat ggctctcgct gtcgaggcgc tagtgggcga aaggattgcc
      181 gggggtatcg tcatcgtcaa acggggacat acggttccac tgcggaaagt agaggttgtc
      241 gaagccggcc acccgatccc ggatcaagcg ggagtcaacg ccactgaaac tatcatcggg
      301 ttgctgaggc ggactcaaaa aacggacttg attctttgcc tcgtttccgg gggggcttct
      361 gcactactgt cgtgtcccgt tgtgggtctg agcttgcagg ataagcagca aaccacgcag
      421 gcgcttctga actgtggtgc gagaattcag gaagtcaatg cgatccgcaa gcacatttcc
      481 agagtcaaag gaggccgact tgccgagctg gcccatcctt cgaccgttct atcgctgatt
      541 ctatcggatg tgatcgatga ctccatggac aacattggtt cgggaccgac ggctcctgac
      601 tcgagcactt ttgcggattg tttgtcgatt attgagggtt atggtgttgg agaaatgatt
      661 cccgtcgccg tgaggagttt gctgaaaaag ggagcagcag gagaaatcgt cgacactcct
      721 aagacgggtg atcccgtttt tcagaaagtc caaaatctcc tggtcggcaa caaccagtcg
      781 gccctggtag cggccaagga aaaagccgag gcgttgggat ataacacctt agttctttcg
      841 agatccattg aaggcgaagc taggaaagtg gcgatggatc acgtggttct ggcaagagat
      901 gtcctatcgg gttcccgccg gattcagcgc ccagcctgta ttatctccgg gggagagact
      961 acggtggcaa tccaaggtaa tggcttgggc ggtcggaacc aagaattcgc cctggcagcg
     1021 gccatcgaaa ttgacggtct aggggaagtg atagtgctca gtggaggcac ggacggcacc
     1081 gacggtccga cggacgccgc cggagggatc gttgacggga ctacagttca gcgcgggaga
     1141 gacaagggac tggatgcgcg agaatatctc aaaagaaacg attcgtatcc ttttctaaaa
     1201 acggtcggcg atttgctagt gacggggccg acactgacaa atgtcatgga cctccgcttg
     1261 atcctcatcg tctagagagg cggcgcgtaa aaaaatgctg tcgcctctca tccgtgccgc
     1321 ctaagacaaa ccctcttccc agccgcttcc aaggtggttg attttgtaaa ccatccgtga
     1381 ttaaagttca ccctttcgga acaaacttgt cttgcacaaa caacgatgcg ttgacgccgg
     1441 atgggagtga gggaaatgat tgacgtcagc ttgcgaggag tccgctgtcg ggcggcccag
     1501 gagattctcg atcttgatgg aaccattggc gctgccaccg agggcacgat tgtctacgaa
     1561 ctcgaaggag aaggaggaca attggtgcac gttcagtggg ataacggaac gagttccctt
     1621 gtctcctcag aggaaattga gattaccgac cgcgcggtga tctggcaatg acagacctac
     1681 gggcgcacac cgggatttaa ctcactttga ccgcgagctt ccgacctaac gctttaaagg
     1741 cgtttagcca ggcctgcgct gctagatcac cctgctcctt ttgccgctgc atgtttggat
     1801 agatgttttc gagaagccgg tagtagctgt tttggatttt ccacaaatcg acgcgaaagg
     1861 gaagggcgcg aatcaaactc gccgcgctgt ccaattgctg caacaagtcg actgtcgggc
     1921 tgacgatcag ccgttccgtc agccgctcga gcttgtgccg ataggcgaat tctaacgtcg
     1981 cggtatccag gggtacgcct tctagttttg cggcttccag cagcgcgttg acgcgctcgg
     2041 cgtcgacttc ttcctgttcc aaagcctggc gcaagtagcc gttgagcacc agctctgccg
     2101 ctgcagaaaa aaccttgggc agcgggatgt gcaggttggt aaggaagcgc atcatcggag
     2161 cacggcgctc atagatttgg cgatacagtg tctccgcctc tccaaggctc gcggtcaaga
     2221 tttgctcgag aactttcctt tgctcgtccc ggaagagcga ccaaagggaa taattggact
     2281 cgccgaagcg ccggtccata aaacgaatca cctcagcgaa gtcagcccgc cgaaaagctt
     2341 ccacggcctc gcgggcaaga cttgggtcgg gctcgttgcc gagagattcc tgcactccgc
     2401 agttgacgtg gtgatcgccc atgtgcagca cgccgaagtt gagcacaatg gattcccagg
     2461 tgatttccga cgtcaacttc acctggccca tggccaattt ggttaggcct gcctcgaaga
     2521 cttggtagtc tcttcgctcg gtccgatagc agcaaatatt cgttcgctcg gggtagcgtt
     2581 cgaaaagaga gctgaccgcg taatgggcac cgatctgctc ccaatcgacc atggccgggc
     2641 gcacgaactt ctcgtagatg cgtcgtccat ctccgtattc gggcaagttg ctctgagccg
     2701 aagcgagtcg ctccatgaac tgcgactcga gtgaatcacc gaagaggtct tgcgccagct
     2761 gtacggcgcg accggcaaac agcatcgcct gcacggtttc gatcccggac agttcatcga
     2821 agaaccagcc gcaactggca tacatcagca tcccatgacg ctgaagctcc agcagtttca
     2881 gagcagtagt aatttcggca gcgctcaatg ggcgaagcgc gtgacgggcg aaaaaacctt
     2941 caacattctc tgtcgaacga tcgtgaatga cgctgaggta gtcattgcgc gcggcccagg
     3001 gatcttttaa aagctcccgg ccctgtttct cgaagggcaa agctagggag tcacgcaatg
     3061 tatccagcgc accccgtaat ggggtgcgcc actcctgatt ccagccggga cggccgccgg
     3121 agttacaacc gcagttgctc caccagcgat cgatgccgtg aaaacaactc caggaggttt
     3181 tttca
//
LOCUS       DASSWZ010000308         7646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1229297, whole genome shotgun sequence.
ACCESSION   DASSWZ010000308 DASSWZ010000000
VERSION     DASSWZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229297"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1870)
                     /locus_tag="VFN58_07405"
     CDS             complement(155..1870)
                     /locus_tag="VFN58_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8917853.1"
                     /translation="MKILDRCKGTLIVLMILTADLVPVTVKSQAPAPARPLVIQGGTL
                     IDATGRPPLQDVVIVIEGERIKAVGKRSDVAIPKGSRIIDVKGKTILPGLIDGHCHLL
                     DFVGELYLSLGVTTCPDITQNDDEWSKAQRDGTQLGKIRGPRIWSTGTRLVGPPPAWA
                     RRAESGHLVKSAEEARAVVRRKKEMGLDIVKFNEYVEPEVIKAGAEEANRLGLPVTCH
                     CLDVFLAAEAGFAGVEHHWAPGMTSISDVKKRWEIHAQRMTGKIDTADLPFHYEAENF
                     DGIIRAMADKQVSWSPTIATWFRPLSPSAARFKERELSILNDPKAGYLPAVMREQTLR
                     QYERYEKFPSQRLNQAREGYTKIADFIVRYVQAGGILRAGSDPNNGLPALGLHQEVVM
                     FVEAGVSPMQAIQAATINVAKAFRKDKDFGTVEPGKVADIIAVEGDPLKDVWTIQNVK
                     MVVLGGKIIDPNFHADYKNPIPNIRPWRATPRDIEIAPRAITQGSGPATLKVSAQRGF
                     DRFHKVTLNGKELETRFVSGGELEASIPPHALKHAGTYAIVVVSQGDFASRSEPAYLI
                     VSFKK"
     gene            complement(1921..2895)
                     /locus_tag="VFN58_07410"
     CDS             complement(1921..2895)
                     /locus_tag="VFN58_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917854.1"
                     /translation="MLRTMTLLAVIIGVFPSKGFGAATSMRVLYPSFAGTWAVAWVAK
                     EAGYFASEGLDVELIRVGGSTRMVAAMLGGSAPIIQAGASAAIAATGAGSDVVIVAAT
                     GKVSPFHLMARPEIKQPSDLRGKRAGITTFGSTSDQVVRIALKHFGLEPNKDVAILTF
                     GAQPEAFAALQNGAVQIAALSYPLYPTAAKLGMRELVKFADLGFEDVNGTVITTKSFV
                     AQQRDTAMRFMRGFIRGMHRYRTDKEYSKRVLGKYGKITDDELLEGTWQDYAPTLQKI
                     PRPSMKAIQFMVENQFKDKTPLPKPESFVDNSLVDQLERSGFIDSVYK"
     gene            3266..3715
                     /locus_tag="VFN58_07415"
     CDS             3266..3715
                     /locus_tag="VFN58_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917855.1"
                     /translation="MAEIINKISLQDGTLVRHKVIGYQGTIEGKTEIKTCFTRGGSVL
                     AIPPAKETFQYRVAVNGESMRHIAPAEDLEILDAMTEVACFRCQYVFRTKPGVLDKPA
                     GRCQCGGWICPACFACQTPEASEQSPCLKQRKRMVKKLANEKKGKRV"
     gene            3867..4877
                     /locus_tag="VFN58_07420"
     CDS             3867..4877
                     /locus_tag="VFN58_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HET8917856.1"
                     /translation="MSARMIVIGTGPIGGIIGGRLARAGNDMTFVDVDQEHVAAIREA
                     GLQVDVPDGPFNVRIPIVFPHEIEGKFDIGFIAVRSNYTPDALATVMPYLADNGLLVS
                     LQNGINPPLLEENVGPDRTIGVAIRMGSLRPSPGHVKTATRGHLYIGHLHGKKTPQLE
                     KLNALLDSVIPSETSDNILGVLWSKLTYTCLGYFGSLADAALAASCKSHAERRMLVDF
                     FAEVVAVGKTAGGRFIRLAEYNPLDFHPDNPFEERLAAVNEMAKAWNVDDRKGPLRQL
                     QKRIKTEVDYTLAHVVREGERLKIPTPLCRKVLDMIHELETGKRKFSQQNYAELAALV
                     KS"
     gene            complement(5113..5703)
                     /locus_tag="VFN58_07425"
     CDS             complement(5113..5703)
                     /locus_tag="VFN58_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917857.1"
                     /translation="MEKLLVTMTGHDPIEKLMPCLEMLAKPGMTVIFVFPYPIDSWSY
                     FRDHRITVESAIHATAVGRKVVQRYNWDAQKELAQRIIAPAVDVLQNKGVEVEIHLCA
                     SSVAKVIKNYSVDKDIHWIISQFPRPGLVGYLSAKSLVPSDWSSHLLSVRADRDSSGR
                     QRQETLPARQRYPVSVWCSGQRVPVSASERKGNNDS"
     gene            complement(5733..6119)
                     /locus_tag="VFN58_07430"
     CDS             complement(5733..6119)
                     /locus_tag="VFN58_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917858.1"
                     /translation="MIHDQFLYCRICHEVHHLTPFDRAPTYQLEGVTVREIFTDDRRQ
                     FVDRHFGHEIEALKSVAENRFYIGRPVDPMKVGYVEVTNGRVSFILRVSRGNIADPVS
                     YNLVPSLVGGRSERLARHVVQERKSE"
     gene            complement(6151..6648)
                     /locus_tag="VFN58_07435"
     CDS             complement(6151..6648)
                     /locus_tag="VFN58_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917859.1"
                     /translation="MVPAQILVLLRRSDPIELFLPYVEQISQPGMRVVFLVQLGRSGF
                     RELSGQLSDIHAGMRSAYLPETSCDEDAVENRIRSAEQQVNAACQSLRQRGVQLEIHS
                     YGGPLQRVVQQYLENQNVQLVMMRPSANWVADLLRKIGSVFRFLKSPAAPPVLLLHPT
                     NIPER"
     gene            complement(6822..>7646)
                     /locus_tag="VFN58_07440"
     CDS             complement(6822..>7646)
                     /locus_tag="VFN58_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET8917860.1"
                     /translation="ELFARAIHNWSERKDRPFVAINCVGLSKELLESELFGYEKGALT
                     GASQLKRGKIEIANGGTVFLDEIGDISEELQAKLLRFLQEREFERVGGNQLIRVDVRI
                     IAATNRNLEAAVKEGRFREDLFYRINVVPIVLPPLRERKEDVQALAQFFMQRFSDEAK
                     KNFAEISQEALETLIGYEWPGNVRELANVIERAIVLGQPPTIQIEDLSPGIVAPEAEV
                     NNRSTPQSYHESIDEYRREVIVNALAQAKGNRAAAARSLGLQRSYLLRLMKTFSIS"
BASE COUNT         1732 a         2143 c         2000 g         1771 t
ORIGIN      
        1 tacaaaggta atttgctgat gttccaggga gggtaataga cgacccttat cgtgtccggt
       61 tgtgcgaagg ctgtgcagcc tatgagcaat tggataaacg taacgatgaa ccggagacct
      121 ttgggtttgc aggtaagcat gggcaatgat ctcctcattt tttgaaggag acgatgagat
      181 aggcaggctc ggagcgcgaa gcgaagtccc cctggctgac gaccacgatg gcatacgttc
      241 ccgcatgttt gagtgcatgc ggcgggatcg atgcctcgag ctcgccgccg ctcacgaatc
      301 gagtctccaa ttccttcccg ttcaaggtca ctttgtgaaa tcgatcgaag ccgcgctgcg
      361 cggatacttt taatgttgcc ggtcctgaac cctgggtgat ggcacgaggg gcgatctcga
      421 tatcgcgggg cgtagcgcgc caggggcgaa tattcgggat tggattcttg tagtcggcgt
      481 ggaagtttgg atcaatgatt tttccgccga gtacaaccat cttgacgttc tggatcgtcc
      541 agacgtcctt cagcggatcg ccttcgacgg cgatgatatc ggcgactttt ccgggttcaa
      601 cggtgccaaa gtctttgtct ttgcggaacg ccttggcaac attgatcgtt gccgcctgga
      661 tcgcctgcat gggtgaaaca ccggcctcga caaacatcac gacctcttga tgcaagccca
      721 aagcgggcag gccgttgttc gggtcggagc cggctctaag aattcctccc gcttgaacat
      781 agcgaacaat gaaatcagcg atcttcgtgt aaccttcgcg agcttgattg agccgctgcg
      841 acggaaactt ctcgtaacgc tcatattgcc gcagggtttg ctcgcgcatc accgccggta
      901 gatagccggc ctttggatca ttgagaatgg acagctcgcg ctctttgaag cgtgccgcgc
      961 tgggagacaa cggccgaaac caggtagcga tggtgggact ccaggagacc tgtttatcag
     1021 ccatggcccg gatgatcccg tcaaaatttt ccgcctcgta gtgaaacgga agatccgcgg
     1081 tatctatttt cccggtcatt ctttgggcgt gaatctccca gcgctttttg acgtcggaga
     1141 tggaagtcat gcccggtgcc caatggtgtt cgacaccagc aaaccccgcc tcggcggcga
     1201 gaaatacatc gagacaatga caggttacgg ggaggcccag acggttggct tcttcagcgc
     1261 cggccttgat gacctcgggt tcgacgtatt cattgaactt gacgatatcg agtcccatct
     1321 ctttctttcg tcgcacgacc gcgcgcgctt cttcggcact tttgacgagg tgaccgctct
     1381 cagcccggcg cgcccaggcc ggcggcgggc cgaccagccg agtacctgtc gaccatatgc
     1441 gcggcccgcg aatcttgccc aactgagtcc cgtcgcgctg cgctttgctc cattcatcgt
     1501 cattttgggt gatgtctgga caggtggtca cgcccagaga gagataaagc tcacccacaa
     1561 agtccaacaa gtggcagtga ccgtcaatca gacccggaag aatcgttttg cctttcacgt
     1621 cgatgatccg gctacccttg gggatggcca catcactgcg tttgccgacg gctttgatgc
     1681 gctcgccttc aatcacgatc accacatctt gcagcggcgg acggccggta gcgtcgatga
     1741 gcgttcctcc ctggatgacc agcggccttg cgggcgccgg ggcctgagac tttaccgtta
     1801 ccggcactag atcggctgtg aggatcatga ggacgatcag tgtgcctttg cagcgatcaa
     1861 gaattttcat tagccattct ccctttcggc tcggttcggg gcccacacgc cctcgagcgc
     1921 tcacttgtaa acggaatcga tgaatccgct gcgttcgagt tgatccacaa gcgagttgtc
     1981 gacaaaactc tccggcttcg gcaacggtgt tttgtccttg aactggttct cgaccataaa
     2041 ttgaatggct ttcatggacg gacgcggaat cttctgcaag gtcggcgcat agtcctgcca
     2101 ggttccttcc aacagctcat cgtcggtaat cttgccgtat tttcccaaaa cacgcttgct
     2161 atattccttg tcagttcgat agcgatgcat gccgcgaata aatccgcgca taaaccgcat
     2221 ggcggtatcg cgctgctgag ccacaaatga ttttgtggtg atcacggtgc cgttcacatc
     2281 ctcgaagccg agatcggcga atttcaccag ctcgcgcatt cccagttttg ccgccgtcgg
     2341 ataaagcggg taactcagcg ccgcgatttg gaccgccccg ttctgcaacg ccgcgaaagc
     2401 ttcaggttgc gcgccgaaag taagaatcgc tacatcttta ttgggctcca acccgaaatg
     2461 cttcaatgcg atccgcacga cctggtcgga tgtggaaccg aaagtggtga tacccgccct
     2521 cttccccctc aaatccgagg gttgcttgat ctcgggccga gccatcaaat gaaatggcga
     2581 gactttcccg gtagcggcga cgatcacgac gtcgctcccg gcgccggttg ccgcaatggc
     2641 ggccgatgct cccgcttgaa tgatcggcgc gctaccgccg agcatggcag ccaccattcg
     2701 ggtgctgccg ccgacccgga tcagctcgac atccagtcct tccgaagcga aatagccggc
     2761 ttccttggcg acccaagcaa cggcccaagt gccggcaaag ctcggataca aaacacgcat
     2821 cgaggtcgcc gcaccgaagc ctttggatgg aaatacaccg atgataaccg ccagcagtgt
     2881 catagttcgc agcatgatat tgcctcgcta gtttttccga tgatcaccat cttacccgtg
     2941 ctccgctccg cgcggctgac ccaaagcccc aacagcaaat gcatcaccaa actaaccgag
     3001 gagttcgtct atcgtcaact gaaacggagg cggtttactg gcggcgacgc cgcgaaacta
     3061 gcagcatgtt cacgatcacg ggagttttcc gatcatcccc acggctcttt gcttttattc
     3121 tttaacagga ggtttttcct ggcgcctggc taattgcgcc gaagaatgtt acagttatct
     3181 tcgatttaga gcttttccat cttcttttcc gagcctctga gctctaaacg agccagaggc
     3241 tttctcgttt tatgaggagg tcgatatggc agaaataatc aacaaaatca gcctgcaaga
     3301 cggaactttg gtgcgtcata aagtaatcgg ctatcaaggg acgatcgaag gcaagaccga
     3361 gatcaaaacg tgctttacga gggggggaag cgtgctggca atcccgccag ccaaagaaac
     3421 cttccaatac cgcgtggcag ttaatggcga aagcatgcga cacattgctc ccgccgaaga
     3481 tttggaaatc ctcgatgcga tgacggaggt ggcttgcttt cgctgtcaat atgtcttccg
     3541 caccaagcca ggcgtattgg acaagccggc tgggcgctgt caatgtggtg gatggatttg
     3601 cccggcctgc tttgcctgcc agacgcccga ggcttccgag cagtctccgt gcttaaagca
     3661 gcgcaagcgt atggtcaaaa agctggccaa cgagaaaaag ggcaaacgag tttgaccggg
     3721 ctctaatctg aactgcctga ttgattcctc tcgtcaatag cgacttctaa tccccgttca
     3781 gctctggaat agagccactt cattgacgcg cctgcgcgct tccaatatct ttggcttgtc
     3841 ttagtgaaat catccaggag gttcacatga gcgcgcgcat gatcgtcatc ggcaccggac
     3901 ccatcggcgg aatcatcggc ggccgactcg cgcgcgccgg gaacgatatg acgttcgttg
     3961 acgtcgatca ggagcacgtg gccgccattc gcgaggcggg actgcaggta gacgtgccgg
     4021 atggcccctt caatgtcagg attcccatcg tttttcctca cgagatcgaa ggcaagttcg
     4081 atatcggttt catcgcagtc cgctcgaact acacgccgga tgcgctggcg acagtcatgc
     4141 cctatctggc agacaacggc ctgctcgtat ccctccaaaa cggaatcaac cctcccctgc
     4201 tcgaagaaaa cgttggaccc gaccggacca tcggcgtcgc gattcgtatg ggttccctta
     4261 ggccgtctcc cggccacgta aagacagcta cccgcggcca cctgtatatc ggacatctcc
     4321 acggcaagaa aacaccccag ctggaaaaac tcaacgcact gctcgactcg gtaattccca
     4381 gcgagacttc ggacaatatc ctcggcgtgt tatggagcaa gcttacgtat acttgcctgg
     4441 gatacttcgg ctcgctcgcc gatgcggcat tggccgcaag ctgcaaaagc cacgcggagc
     4501 gtcgcatgct cgtcgacttc ttcgccgaag tagtcgccgt cggcaaaacc gccgggggtc
     4561 gtttcatccg actggccgaa tataatccgt tggactttca ccccgacaac ccctttgaag
     4621 aacgccttgc ggctgttaat gagatggcca aagcatggaa cgtcgacgat cgcaaagggc
     4681 cgcttcgcca gcttcagaag cgcatcaaga ccgaggtgga ttatacccta gcccacgtgg
     4741 ttcgagaggg agagagactc aagattccga cgccgctctg ccggaaggtc ctggacatga
     4801 ttcacgagct cgagacggga aagagaaagt tctcgcaaca aaactacgca gaacttgcag
     4861 ctttggttaa atcataagac cgattcttac aaagaggaca agcgctcaat gaggcaattc
     4921 attttacagg ctagtttctt taggtcgtct atgctgtcct cattgggaac cgtaggaccc
     4981 cgatcagtct ccaacgtttc aacccaataa agcaatatac tcatacagtt tttgagatca
     5041 tgacaaacct cggaaagctg acgaggagtt tctcccgctc ggaccgagac gggcaggcct
     5101 tggagaagat tttcagctat cattgtttcc ctttctttcg ctagcactca ctggaactct
     5161 ttgacccgaa caccagaccg aaacagggta cctttgcctc gccggtagag tctcctgccg
     5221 ttgtctcccg gaagagtccc ggtcggctcg cacagacagc aaatgactgg accagtctga
     5281 gggcaccagg cttttggctg acaaatagcc caccaggccg gggcgcggga attggctgat
     5341 gatccaatga atgtccttat caacgctgta gttctttatg accttagcca cactgctcgc
     5401 acacagatgg atttcgacct cgaccccttt gttctgcaag acatcgacgg ctggagcgat
     5461 gatccgctgg gctaactcct tctgcgcgtc ccaattgtat cgctgcacta cctttctacc
     5521 gaccgccgtt gcgtggattg cagattcgac ggtaattcga tgatctcgaa aataagacca
     5581 tgaatcgatc gggtatggga aaacaaagat cacggtcatt ccaggctttg ccaacatctc
     5641 aaggcaaggc atcaactttt ctatgggatc atgccccgtc atggttacta gtaacttttc
     5701 catttcgggc ctcgtggcaa ttcctatagc ctctattctg attttcgctc ctgtaccacg
     5761 tgccgtgcca acctttcaga acggccacca acgagactgg gtacgagatt gtaactcacg
     5821 ggatcggcga tattgcctcg agaaacccgc aggataaaag acactcgtcc attcgttact
     5881 tcaacgtacc ctactttcat tggatcgacg ggccggccga tgtaaaaacg gttctctgcc
     5941 acacttttca acgcctcgat ctcatggcca aaatgcctat cgacaaattg acgccgatca
     6001 tcggtgaaaa tttcccgcac agtcactcct tccaactggt aagtaggcgc acgatcgaaa
     6061 ggagtgaggt gatggacctc gtggcaaata cggcaataaa gaaattgatc atgaatcatg
     6121 gtttctgcag cggtttatcg ataggccctt ttacctctca ggtatgttag tgggatggag
     6181 aaggagaacc ggaggggcag cgggcgactt caaaaaacga aaaacagatc cgatcttgcg
     6241 cagaagatcc gccacccaat tggcactcgg acgcatcatc accagctgga cattttggtt
     6301 ttcgaggtac tgctgcacga ccctctgcag cgggccccca taagaatgta tctcgagctg
     6361 cacgcccctc tgccgcaatg actggcaggc tgcattaacc tgttgctcag ccgatcgtat
     6421 tctattctcc acggcgtctt cgtcgcagct tgtctccgga agataggcag atcgcattcc
     6481 ggcatggata tcggataatt ggccgctcaa ttctctaaac ccgctccggc caagttggac
     6541 aaggaaaacg actctcattc cgggttggga aatttgttcg acgtagggga ggaatagctc
     6601 aatcggatca gatctcctca acaggactag tatttgcgct ggcaccatcg ctcctcccat
     6661 tacgctctac agttgttcaa aaagcaggga ctatgccaat tggaaaggat ggcctttgct
     6721 gagtccatca attgaacttt tcaggagaat cgactcgacc gatgcttaat cggcagccga
     6781 taatagtatc gttaaagata cgacggtgta taaaataaga ttcaggagat gctgaaggtt
     6841 ttcatcaacc ttaagagata acttctctgc aggcccaacg accgagcggc cgcggcgcga
     6901 tttcctttgg cctgcgccaa tgcattgaca attacttcac ggcggtattc atcgatgctc
     6961 tcgtgatagg actggggggt tgagcggttg ttaacttctg cctcgggggc gacgattcct
     7021 ggagacagat cttcgatttg gatggttgga ggttggccga gaacgatggc cctttcgatg
     7081 acatttgcca gctcacgcac gtttcccggc cactcataac ctatgagcgt ttccaaggcc
     7141 tcttgagata tctccgcgaa gttctttttc gcctcatcag aaaatcgctg catgaaaaac
     7201 tgggccagag cctgcacgtc ttctttgcgc tctcttaagg gagggagcac gatgggaacg
     7261 acgttgatgc ggtaaaataa atcttcacga aaacggccct ctttgaccgc ggcttcgagg
     7321 ttgcggttcg tggccgcgat gattctcacg tcgacccgaa tgagctggtt gccgcccacc
     7381 cgctcaaact cccgctcctg caaaaatcgc aacagcttag cctgaagctc ctcggagata
     7441 tcaccgattt cgtcgagaaa gactgttcca ccgttggcga tttcgatctt ccccctcttg
     7501 agctgactgg caccagtaag ggcgcctttt tcgtagccaa acagttcgct ttccaacagc
     7561 tcctttgaaa gaccgacgca attgatggca acaaagggcc gatcttttct ttcgctccag
     7621 ttgtgaatcg ccctggcgaa gagttc
//
LOCUS       DASSWZ010000309         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_210380, whole genome shotgun sequence.
ACCESSION   DASSWZ010000309 DASSWZ010000000
VERSION     DASSWZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210380"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1608
                     /locus_tag="VFN58_07445"
     CDS             115..1608
                     /locus_tag="VFN58_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET8917861.1"
                     /translation="MTRPAANFKIVEATIDDVHSAYKSGDLTCRQLIQMYLDRIAAFD
                     KKGPAINAIITINSKALEEADRLDAAYNSSGPAGTLHGIPVVLKDQVDAKGMPTTLGS
                     VLFKNYYPDKDAFVVQKLKEAGAIILGKATLGELGGGDTHGSLFGSTRNPYALARTVG
                     GSSGGSGASVSANFTTVAVGQEGLASIRRPSTWNSIVGMRTTAGLVSRGGVYSGWPET
                     AGTLGPMARTVRDLALLLDVMVGYDSEDPLTARGVGNTPDSFTGFLDKNGLKGARIGV
                     LRESMGYNSEPDSDDFRQVTEVFDNAIAELKSAGATIVDPIVIPKLNELLEKRAGNPT
                     AGEEAFRLYFGRSSHPPFKSRAEAMQSTDFAQVSRGARNRLRSSSNPEKYYQYLIARD
                     ELMINVLKVMADEKLEAVVHKSIEHQPTLISDGVNPPYVNHRGAPHLNTFLVFVPTIV
                     VPAGFTRDGLPAGISFLGRPFDDGRMIKLAYSYEQATRHRRPPASTN"
     gene            complement(1663..2703)
                     /locus_tag="VFN58_07450"
     CDS             complement(1663..2703)
                     /locus_tag="VFN58_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917862.1"
                     /translation="MKPTWRIFEIGLFVALVSVIGRPATVNSQSKDIIEGAKKEGRVL
                     LYTALTIHEIDEIAVPFVKKYPFLKVEPFRGNALQLLQKITMEARAGRAQVDVAQFNG
                     FEAWQLQKLGLLQSYKSPEAKNYPDSYKDPGGYWTTLYANYLVLGFNPKMVPAADVPQ
                     KWDDLLHPRWKGNKLALDRDNAVWYSGLALYWGKEKADKFMRALADQNPTMRKGHTLI
                     ATLLSSGEFPLGLVYAHRVEEMKAKGISTIEWLPLEPIVATPNVIGIGKSAPNSNAAK
                     LFVDFFLSKEGQTISQKQQYRVPANPDLPPVSPNLDPKNLKISLVNRQAAESYEQFDK
                     AYQELFVKSKAE"
     gene            complement(2911..>3661)
                     /locus_tag="VFN58_07455"
     CDS             complement(2911..>3661)
                     /locus_tag="VFN58_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917863.1"
                     /translation="GGTAISRAIGTGSFDLVVVATETRYVPMRLMVNSSIRSPIDLKG
                     KKLGVGRAGLDEYAALLYLERVGLVPGKDVQIVYMAGGIPNRATAMKQGLVNGVIVNP
                     PNEYELEKAGYFELANFLDFKMPYAGVPQTFSKVFRDRNRKVVEDYITAIVEGMQIFR
                     THRETGYKAIYEATRQNDPVILERTYVSYLRQYEAIGGLPFPWQSGIEGMITGFHERF
                     NPQGIKNRDARPFLDPSFVQKAAERLKLDRK"
BASE COUNT          866 a          957 c          941 g          897 t
ORIGIN      
        1 aagcccgcca ctgccaggac atgatttttg tttttgagtg ttgccatctc cgtgatggtt
       61 ttgcacggct tacttgagta agtcaatctt tgtcggaaca agggagatac tggaatgaca
      121 cgacccgcag caaactttaa gattgtcgag gcgaccatag acgacgttca cagcgcttat
      181 aagtccggcg atctcacgtg ccggcaacta atacagatgt atctggatcg catcgcggca
      241 tttgacaaaa aaggacccgc tatcaatgca atcatcacaa taaactcaaa agccctcgaa
      301 gaagccgatc ggcttgacgc cgcctataac tcctccgggc ccgcaggtac gcttcatggc
      361 attcctgtag tcctaaaaga ccaggtcgac gccaaaggca tgccgacaac tttaggttcg
      421 gtattgttca agaattacta tcccgataaa gatgcttttg ttgtacagaa actcaaagag
      481 gcaggagcaa ttattttggg aaaggccacc ctgggcgaat tgggaggcgg cgacacccac
      541 ggatcgttgt ttggatcgac gcgcaatcca tacgcgttgg ctcgaacggt cggcggttcc
      601 tccggcggct caggcgcaag cgtgtccgcg aacttcacta cggtagcggt aggacaagag
      661 ggtttggcgt caatacgtag accatccacg tggaactcaa ttgttggaat gcgaacgacg
      721 gccgggctcg tgagccgcgg cggggtctat tctggttggc cagaaacggc cggcactctc
      781 ggcccgatgg ctcgcacggt gagggatctc gctctgctat tggatgtcat ggtgggatac
      841 gactcagagg atcctttaac tgcgagaggg gttggcaaca ctccggacag cttcaccggc
      901 tttctagata aaaacgggct caagggagcg cgtatcgggg ttttgcgcga atccatgggt
      961 tacaattccg agcctgattc ggacgatttt agacaagtca ctgaagtttt cgataatgca
     1021 atcgcagagc taaaatccgc cggcgccacg attgtcgatc caatcgtgat tccaaagcta
     1081 aatgaattgt tagagaaacg cgccggtaat cctacggccg gcgaggaagc ctttagattg
     1141 tattttggca ggagtagcca ccccccgttc aagtcgcgcg cagaggcaat gcagtcgacc
     1201 gatttcgcgc aagtctccag aggcgcgcga aatcgcctgc ggagttcctc gaacccagaa
     1261 aaatactacc agtacttgat cgcgcgggac gagttgatga tcaatgttct aaaagtgatg
     1321 gcggatgaga agctcgaagc cgttgttcat aaatccatag agcatcaacc gacactgatc
     1381 agtgacggag ttaatcctcc gtacgtcaac cataggggcg cgccgcactt gaataccttc
     1441 ctagtatttg taccgacgat cgtggtgccg gcgggattta cgagagatgg ccttccggcc
     1501 ggcatcagtt ttctgggacg tccttttgac gatggcagaa tgattaaact tgcatattca
     1561 tatgagcagg caacccgcca tcgccgtccg ccggccagca caaactaagg ttgagtcggt
     1621 tttgcgcaac gccaccggat tggtttgcgt cggttcgtaa cttcattccg ccttgctttt
     1681 tacgaaaagc tcctgatacg ccttgtcaaa ctgctcatag ctttcggcgg cctgccggtt
     1741 caccaaagaa atcttgaggt tcttaggatc caggttaggc gaaacaggag gtaaatccgg
     1801 gtttgcaggc acccgatatt gctgcttttg agagattgtt tgaccttcct tcgagagaaa
     1861 aaaatctaca aacaactttg ccgcgttgga attaggggcg ctttttccaa tgccgattac
     1921 attcggcgtt gcgacgattg gctcgagcgg aagccactcg atagtactaa tcccttttgc
     1981 tttcatttcc tcgacccggt gtgcatagac caaccccagc ggaaattctc cgctggataa
     2041 tagggtagca atcaatgtgt gccctttacg catggtcggg ttttggtcgg ccaaagctct
     2101 catgaacttg tcagcttttt cctttcccca ataaagcgcc aacccgctgt accaaacggc
     2161 attatcccgg tcgagggcaa gtttgttacc tttccatctg gggtgaagaa ggtcatccca
     2221 tttttgcggt acgtcggccg caggtaccat ctttggattg aagccgagca ccagatagtt
     2281 agcataaagc gttgtccagt aaccccccgg gtccttgtaa ctatcaggat agtttttcgc
     2341 ttccggggat ttgtagctct gtagcagtcc taacttctgc agttgccaag cttcaaaccc
     2401 gttgaattga gccacgtcaa cctgcgcacg cccagcgcga gcctccatag taattttctg
     2461 aaggagctgt aaagcattgc ctcgaaaagg ctctaccttt aaaaatgggt acttcttgac
     2521 aaagggaact gcgatctcgt caatttcgtg gatggtcaat gcagtgtaca gtaggacccg
     2581 gccctctttt tttgctcctt cgataatatc ctttgactgg ctgttgactg ttgctggtcg
     2641 accaatcacg gagactaatg cgacgaaaag gccgatttca aaaattctcc acgttggttt
     2701 catggattcc tcctctggca actaagctgt gcgcactgaa gcaaatcgag aagtcttaaa
     2761 cccgcgtatg ccacgtatac gcctcccacg aagaactcgt caatgacttc ttatagtata
     2821 gacgtgattt tggatgtgag ttttgagtga gtccgggctc aaagtgcaga cattgacctt
     2881 tgatccagcg accgcctggc caacaggcat ctactttctg tccagcttca aacgttcggc
     2941 ggctttttgc acgaaggatg gatctaggaa gggtcgcgca tctcgattct tgattccttg
     3001 gggattaaag cgctcatgaa aaccggtgat cataccctcg atgccggatt gccacggaaa
     3061 cggcaaaccg ccgatggcct cgtactgcct aaggtaagaa acataggtcc gctcgagtat
     3121 tactggatcg ttctggcggg tcgcctcata gatcgctttg taacccgttt cccgatgggt
     3181 ccgaaaaatc tgcattccct cgacaatggc agtaatatag tcttccacca ctttgcggtt
     3241 tcggtcgcga aagactttgc tgaaggtttg cgggaccccc gcgtaaggca tcttgaaatc
     3301 gaggaaattc gccagctcga aataaccggc tttttccaac tcgtactcgt taggagggtt
     3361 tacaatcact ccgttaacaa gcccctgctt catggcggtc gcgcggttgg gaatcccgcc
     3421 agccatatag acgatctgaa cgtcttttcc cggaaccaaa cccactcttt cgagatagag
     3481 caaggcagcg tactcgtcga ggcctgcccg gccgactccc agcttcttgc ccttcaaatc
     3541 gatcggactt cggatcgaag aattgaccat tagccgcatg ggcacgtagc gcgtttccgt
     3601 cgcgaccacc accaagtcga aactgcctgt gccgatggcc cgggatatgg ccgtcccgcc
     3661 g
//
LOCUS       DASSWZ010000310         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1257769, whole genome shotgun sequence.
ACCESSION   DASSWZ010000310 DASSWZ010000000
VERSION     DASSWZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257769"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VFN58_07460"
     CDS             <1..144
                     /locus_tag="VFN58_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET8917864.1"
                     /translation="GKSFDVKVYPGAKHAFNNNTNADRYHPEAAKDAWARTVSFFKKN
                     LAA"
     gene            complement(208..543)
                     /locus_tag="VFN58_07465"
     CDS             complement(208..543)
                     /locus_tag="VFN58_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917865.1"
                     /translation="MAKRIRTEFALIALAMVLVGTLPSLLRAEETTIEKAGVALGVSA
                     GNVIFLPAKAISTSMGVFFGALSFVLTGGDTEIMRQAWRDSTEGPYLITPEVARNAIG
                     RRPNLSETE"
     gene            complement(548..955)
                     /locus_tag="VFN58_07470"
     CDS             complement(548..955)
                     /locus_tag="VFN58_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917866.1"
                     /translation="MASPITEQNRRKGESTRETIAKPPVAATQLDSHNHFHRPEPRQT
                     LTETKRLYLRVGDEVTHLRYEEWGMGVVMEIMTSSVPGGTCLARVRFQDGQLRCFNND
                     LDNESCCYYFGVRRYWNPSHGVNAVRSKFFLRG"
     gene            complement(1107..1991)
                     /locus_tag="VFN58_07475"
     CDS             complement(1107..1991)
                     /locus_tag="VFN58_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET8917867.1"
                     /translation="MTQARDKFIDVQGLAIHYLEWGEPGGEPLVLIHGFLDQAASWQP
                     FVRSLQNKLNQPLWVVAPDCRGHGGSGWVGAGGYYHFPDYVLDLDCVVRALGVERLKL
                     IGHSMGGTISFLYSGTFPKKVKQLVLIEGIGAEGMSFSDAPVRMEKWITELRDRGRNH
                     FRQYSSVAAGAKQLQQTNPRLSSELALDLAHSGMKQNAKGRWVWKFDPLHRTISPQPF
                     YSEQAMEFFRRIECPVLIVDGKQSRHSHRADKKQWLAAVRDKHFAEIDSAGHMVHLDN
                     PDGLADQILPFLYSKGVL"
     gene            complement(1997..>2419)
                     /locus_tag="VFN58_07480"
     CDS             complement(1997..>2419)
                     /locus_tag="VFN58_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HET8917868.1"
                     /translation="GQHAQEAFPEECCGVILTDGAVDQVHRLKNIQNQLHALDPQTYP
                     RTATIAYAMDYKELELVTDEAKRNGARLKAFYHSHPNHDAYFSAEDKAFASPFGEPTF
                     PGTAQIVVAIYDRAVKEMCAFAWSDQEMDFIKVTLTKT"
BASE COUNT          543 a          638 c          657 g          581 t
ORIGIN      
        1 ggcaagagct tcgatgtcaa agtttatccg ggcgcgaagc acgcgttcaa caacaatacc
       61 aacgccgacc gctaccatcc ggaggcagca aaggatgcgt gggcgaggac ggtaagtttc
      121 tttaagaaga acttggcggc ctaagcgggc gctaatgaga tcgtggcatg tggtcttgcc
      181 tgatcaggca aggctacatg cccgaggtta ctcggtctct gaaaggttag gtcgccttcc
      241 gatcgcgttt cgagctactt ccggagttat caggtaagga ccctcggtgc tgtcccgcca
      301 agcttgtcgc attatctcgg tatccccgcc tgtcaggaca aacgataagg ctccaaagaa
      361 cactcccatc gaggtactaa tcgccttggc gggtaggaat attacattcc ctgcgcttac
      421 gcccagcgcg actccggctt tttcaatcgt cgtctcttca gcgcgtaaca acgaaggcaa
      481 tgtaccgact agaaccatgg ccaaggcaat caaagcgaat tcagttctga ttcgtttagc
      541 cattggacta acctctgaga aaaaatttcg accggacagc attgacgccg tgggacggat
      601 tccaataacg ccgcaccccg aagtagtagc agcaactctc gttgtctaga tcgttgttga
      661 agcagcggag ctgcccgtct tgaaaacgga cacgggcgag gcatgtcccg cctggtaccg
      721 atgaggtcat gatttccatg accactccca tgccccattc ctcgtagcga agatgagtca
      781 cctcatcacc gacgcggaga tagagtcgtt ttgtttcggt gagtgtttgc cgtggctccg
      841 gtcggtgaaa gtggttatgg gagtcgagtt gcgtcgccgc cacgggtggc ttggcgattg
      901 tctcgcgagt ggactctccc tttcgtctat tttgttctgt aatcggagaa gccatgaact
      961 ccgactttgc attagatggc tttgtacgtt tcacagatca cttttctcgc atcgtgtttt
     1021 cggcaacgtt tcaacgtgcc gcataaccca taatcaccga atttgggtat gagagtcaag
     1081 ctctaccgat tcccagctcg aaacatttat aacactcctt ttgaataaag gaagggcaaa
     1141 atttggtccg caaggccgtc tggattatcc aggtggacca tatggccggc gctgtcaatc
     1201 tccgcgaaat gtttatctcg gacggccgcc aaccattgct ttttgtcggc acggtgggag
     1261 tggcggctct gtttgccatc gacgatgaga acggggcatt cgatgcggcg aaaaaactcc
     1321 atggcttgct ctgagtaaaa gggctgaggg gagatagtgc gatggagcgg atcaaatttc
     1381 cagacccacc tgcccttggc attctgcttc attcctgaat gcgccaagtc cagtgccagt
     1441 tcgctgctca gacgcgggtt ggtttgttgg agttgcttgg cgcctgccgc cacactgcta
     1501 tattgacgga aatgattgcg gccgcgatcg cgaagttctg taatccattt ttccatacgc
     1561 accggcgcgt cggaaaaact catgccttca gcgccgatgc cttcgatcaa cacgagctgc
     1621 tttacttttt ttggaaaggt gccggaatag agaaaagaga tcgtgccgcc catggagtga
     1681 ccgatgagtt tgagccgctc cacccccagc gcgcggacca cgcaatccag atccaaaacg
     1741 taatcgggaa aatgataata accccccgcg ccgacccagc cgctgccacc gtgtccacgg
     1801 cagtccggcg caacaaccca cagaggttgg ttgagcttat tttggaggga tcttacaaac
     1861 ggttgccaac tcgccgcctg atctaaaaag ccgtggatta agactaacgg ttctccaccc
     1921 ggttctcccc attccagata atggatggcg agtccctgga cgtcaatgaa cttgtcgcgg
     1981 gcctgggtca cgaggactaa gtcttggtta aagtgacttt aataaaatcc atttcctggt
     2041 cggaccaggc gaaggcgcac atttccttga cggctcgatc gtaaatagcg acgacgattt
     2101 gcgctgttcc tggaaacgtg ggttcgccga atgggctggc gaaggccttg tcctccgccg
     2161 aaaagtaagc gtcgtgattg ggatgggagt gatagaaagc tttaagcctg gcgccgttcc
     2221 ttttagcctc gtcagtcacg agctccagct ctttgtaatc catcgcgtag gcgatggtcg
     2281 ctgtgcgcgg gtaggtttgc gggtcgaggg cgtgcaactg attttggata tttttcaagc
     2341 ggtggacttg atcgacagcg ccatcggtta agatcactcc gcaacactct tcaggaaaag
     2401 cttcctgggc atgttgacc
//
LOCUS       DASSWZ010000311         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_323761, whole genome shotgun sequence.
ACCESSION   DASSWZ010000311 DASSWZ010000000
VERSION     DASSWZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323761"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFN58_07485"
     CDS             <1..316
                     /locus_tag="VFN58_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET8917869.1"
                     /translation="RERCPESRFELVFCNVAGEPLDRTMIGREVLAPAIERAGIQKKI
                     TPHSLRHTYASTLIMLGRDVAQVSKYLGHSDVYVTMAIYTRFVERKHDTMDDLEQLVQ
                     LS"
     gene            416..502
                     /locus_tag="VFN58_07490"
     tRNA            416..502
                     /locus_tag="VFN58_07490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Leu,seq:cag)
     gene            complement(1036..1734)
                     /locus_tag="VFN58_07495"
     CDS             complement(1036..1734)
                     /locus_tag="VFN58_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET8917870.1"
                     /translation="MEVLHFFLRWIHFLAGITWIGILYYFNFVQTPFFAETEAPVRTG
                     AIQKLVPRALWWFRWGAMVTFLAGLLMYLIRWHQMGGEAFFSSQYGVTITLGGLLGTI
                     MFLNVWLVIWPNQQIVIASANQAAQGGQPLPAAAGAGRRAGLTSRTNTVLSIPMLFFM
                     GAAMHYPGLVGPSPAQGFFWVITLIIIAAVEYNALVGTQGATKKPLDSVSGALWVGFL
                     LTGIFLVLALGLLR"
     gene            2024..3040
                     /locus_tag="VFN58_07500"
     CDS             2024..3040
                     /locus_tag="VFN58_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET8917871.1"
                     /translation="MKATVAVAPETRRKIPSLEGSSDPSSFVTDELQLVEDNLIRAIR
                     SREQTLTDIAAHLICGGGKRVRPIVTLLAFNAFGGKRTQDIVDIATAIELIHTATLLH
                     DDIIDRAETRRGKESAYRKFGLKNTLVAGDFLFIKAFEFAGKFDETIVEWTADACALL
                     TEGETLQGYFNRNRAVTLDDYIEIVKRKTASLFQTGAKVGAYLAGANSGLVEETHRYG
                     LNMGIAFQMVDDILDVIGHVDLLGKPTGMDLRDGNPSLPIILALKAEQTAVCEAFQSA
                     QPTEALVGAALNAIKSGPLIQQAKSISRDYAEEALKSIRKFPPSLYRNGLKTLVQLII
                     DRDF"
     gene            3102..3839
                     /locus_tag="VFN58_07505"
     CDS             3102..3839
                     /locus_tag="VFN58_07505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HET8917872.1"
                     /translation="MTSLDDPRDRAVQAMFDRIAGRYDLLNRVISFRLDSVWRTKVIK
                     EVLKSEEPLILDLGSGTGDLTFSAATTVKGNGRIVGLDFSLPMLLLARSKQRSAKHGE
                     KTHFIQGSALFPPFKDCVFDGVMTAFVLRNVSDLSLFFTHAFRVLKDGGRFVSLDMFP
                     PSKTWFSAFYSLYFYRLVPWIGGLLAHDHSAYRYLAESVESFYSPQTVAELLEQAGFR
                     QVSTRKFLHGAVCMHVAEKLNASPHTS"
     gene            3844..4674
                     /locus_tag="VFN58_07510"
     CDS             3844..4674
                     /locus_tag="VFN58_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917873.1"
                     /translation="MRSFSSSFLLFFYQVALGGLFGLSATPFHELERAFYKSTAGVLF
                     AIAVLGLWGKSHLYWQSIAVDIGFATLAEILFHALFVISFAAYMISLWGENQEFRARS
                     FATCVLCGLTGLIFSAHGFYQAPFWSFETFVYPIAFFLSALLLGTVTVGMLLGHWYLI
                     DTGQSLDPFIRIYKFFVGALVVQTIFLLMSVIALHMFGESASVIGLRRLWVDHRTLFL
                     TRVIVGQAAPLILSGMIWRTLVIPNTMAATGLFYIALLGVFVGEILGKQILALTSLPF
                     "
     gene            complement(4694..4966)
                     /locus_tag="VFN58_07515"
     CDS             complement(4694..4966)
                     /locus_tag="VFN58_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917874.1"
                     /translation="MSDNEDNLAPPLGEDYYHHGVIVKLFPSNNMGVVRTESGREVRF
                     SYDLVILSGEAKTPLDLREGERIGYDLGWTSSGLRITKIKTYPRVG"
     gene            complement(5144..5593)
                     /gene="nuoB"
                     /locus_tag="VFN58_07520"
                     /pseudo
     CDS             complement(5144..5593)
                     /gene="nuoB"
                     /locus_tag="VFN58_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
BASE COUNT         1380 a         1520 c         1418 g         1344 t
ORIGIN      
        1 gcgcgagagg tgccctgaaa gcagattcga gcttgtgttc tgcaacgtag ctggtgagcc
       61 gcttgatcgt accatgatcg ggcgcgaggt gttggcgcca gcgattgaga gagccggaat
      121 ccaaaagaag atcacaccgc actcgctccg ccatacctac gcgagcactt tgattatgct
      181 aggcagagac gttgcgcagg tgtcaaagta cttgggtcac tccgatgtct atgtcaccat
      241 ggcaatctat acgcgcttcg tagaacgcaa gcacgacacc atggacgact tagagcagct
      301 agtgcagttg agttgagggt aaacacgatt gagtcaaatc tatatggcac gccaaggcac
      361 ggatttgacc cagaaaggaa aacacggtaa ggctaactag ttgatattgc gagatgcgaa
      421 agtggcggaa ttggtagacg cgcaggattc aggatcctgt ggggtaacac ccgtaggggt
      481 tcgactcccc tctttcgcac cagataaaat ttagcacaca agcctcgctt tctctaacat
      541 ctacattaat cgtactgtgg atgcggaggt tggcctatcg tccgcttgca cacgtcggct
      601 cgtcatcgat gggcgaagga gcagattgat ctaattctca atgctaaaaa accacaacgg
      661 tcgcttagat tactcgttag aatgccccag gaaaaacgat gaaaccatcg aggagttttt
      721 tgctgatagt tcttttgact ttagcagcga gcgcgtgctc taaagaatca gagcaggcgg
      781 gtcaaagtcc ggactggaaa agaggcaggg ccgtttacgt tgcgaactgt gttgcatgtc
      841 ataacaacga tccgtcaaaa gatggcccca tcggccctgc catcaagggt tcaccgccag
      901 aattgattga atctcgagtt ctacgcaccc aatatccccc cggatataaa cccaaacgaa
      961 atacccaggt catgcccact ttcccgttcc tcaaaaccga gattccctat ctcgtagttt
     1021 acctgcgttg acagattacc ttaagagacc tagcgccaag acaaggaaga ttcctgtcag
     1081 caagaaaccc acccacagag cgccggaaac ggagtccaag ggtttcttgg tagcaccttg
     1141 ggttccaacc aacgcgttat actccaccgc agcgatgata ataagagtga tcacccagaa
     1201 aaaaccttgg gcaggcgagg gtcccaccag tccgggataa tgcatcgcgg cccccatgaa
     1261 aaagagcatg gggatggaga gcaccgtatt tgtcctcgac gtcagcccgg cgcgccgccc
     1321 cgcacccgcg gcagcaggta agggctgccc tccttgcgct gcttgattcg ccgatgcgat
     1381 gacaatctgc tgatttggcc aaatcacaag ccagacgttc aaaaacatga tggttcccag
     1441 caaaccgccc agggtaatcg taacgccata ttgagaactg aaaaaggctt ccccgcccat
     1501 ttggtgccac cggattaaat acatcaaaag acccgcgaga aaagtcacca tggcacccca
     1561 gcgaaaccac catagcgcgc gcggtacaag tttctggatc gctcctgttc ttacgggcgc
     1621 ttccgtttca gcgaagaacg gagtctggac gaaattgaaa tagtacagaa ttccaatcca
     1681 cgtaatacct gccaagaaat gaatccacct aagaaaaaaa tgaagaactt ccatagctct
     1741 cccctccttc taccctcgac gatacatcaa agggattgaa cggcccacga tttggctttt
     1801 tatatattag cggatgctca tttttgtcaa ggaaaaaaca cgcctctgta gcgccggacg
     1861 aggcgaaacg tagattagga tctacaagat taaccaccgc accttttgcg cggcggcgct
     1921 tgaagataag cttgacctga ctatagaact gtgccatttt gcgtgctatc ttttgtctcg
     1981 gcttcatgga gtttttctga ttcgggaaac aaaaactaag gccgtgaaag ctaccgttgc
     2041 cgtagcccct gaaacgcgac gcaaaatccc ctctctcgag ggatcgagcg acccttctag
     2101 ctttgtgacc gatgaactgc aacttgtcga ggacaacctg attcgggcga ttcgctcgcg
     2161 cgagcaaacc ctcaccgata ttgcagctca cttgatttgc ggtgggggca aaagagttag
     2221 gcccattgtg actctcttgg ccttcaatgc tttcggtggc aaaagaaccc aagacattgt
     2281 cgacatcgcg actgcgatag aacttattca taccgcgaca ctgcttcacg acgacattat
     2341 tgacagagcg gagacccggc gcggcaagga gtccgcttat aggaaattcg ggctcaagaa
     2401 cacgctggtt gcgggggatt ttctttttat taaggcgttc gagttcgcgg ggaagttcga
     2461 tgagacaatt gtcgagtgga cggcggatgc ctgcgccctt ctcactgaag gggaaacgct
     2521 tcaaggttac ttcaatcgca atcgcgctgt tactctggac gactatattg aaatcgtaaa
     2581 gcgcaaaacc gcctccctat ttcaaaccgg cgccaaagtg ggagcctatc ttgccggggc
     2641 aaattccggg ttggtggaag aaacgcatcg ctatgggctc aacatgggaa tcgcgtttca
     2701 gatggtcgat gacattctcg atgtcatagg ccatgtcgac cttctcggca agcccaccgg
     2761 catggacctc cgagatggaa atccctcgtt gccgatcatc ttggcgctca aagcagagca
     2821 gaccgcagtt tgcgaagcct ttcagagcgc gcaacccacg gaggcgctgg taggtgcggc
     2881 actcaacgcc atcaaatcgg ggccccttat ccaacaggcc aagtccatat cccgggatta
     2941 tgctgaagaa gcactcaaat caattaggaa gtttcctcct tcgctctatc gcaatgggct
     3001 caagacgctt gttcagttga ttatcgatcg agatttttga gtcataacac tgcagttatt
     3061 tgccctgttc acgagtgccc ggtcaatttt gaacccgagt gatgacatcc ctcgatgatc
     3121 cacgcgatcg cgcagttcag gcgatgttcg accggattgc ggggcgctat gatctcttga
     3181 atcgcgttat cagttttcgc ctggacagcg tgtggagaac caaagtcata aaggaggttc
     3241 tgaaaagcga ggagccgctc atcttggatc tgggttcagg gacgggtgat ctcaccttca
     3301 gcgcagccac cacggtcaaa ggcaacggac gaattgtcgg gctggatttt tccctaccga
     3361 tgctcctgct ggcgcgctcc aagcagcgca gcgctaaaca cggcgaaaaa acgcatttta
     3421 ttcaaggcag cgcgctattt ccaccattca aggactgcgt atttgatggc gtcatgaccg
     3481 cgtttgtatt gcgcaacgtg tcagacctct ctttgttctt cactcatgcg ttcagagtcc
     3541 tcaaggacgg cggaagattc gtgtcgttag atatgtttcc gccatcgaag acctggttct
     3601 cagcgtttta ctcgttatac ttttaccgtt tggtcccctg gatcggcgga ctcctggccc
     3661 atgatcacag cgcttacagg tatctagccg agtccgttga aagcttttat tcaccgcaga
     3721 cagtcgcgga actgctcgaa caggcgggct tcaggcaagt gagtacgcgc aagtttctcc
     3781 acggggcagt ttgcatgcat gtggctgaga aacttaacgc atcgccgcat acatcgtaag
     3841 gttatgcgga gtttttccag cagttttctt ctgtttttct atcaggtcgc attgggtggc
     3901 ctctttggac tgtcagctac gccctttcac gaactcgaaa gagcattcta taaatccacc
     3961 gccggcgttt tatttgcgat agctgtgctg gggctctggg gaaagagcca tctgtactgg
     4021 cagagtatcg ccgtcgatat tggctttgcg actctagcag agatcttgtt tcacgccctg
     4081 tttgtgattt cttttgccgc ctatatgatc tctctctggg gtgaaaacca agaattcaga
     4141 gcgcggagct ttgcaacgtg cgtgctctgc ggcttgaccg gactgatttt tagcgctcac
     4201 ggcttttacc aagctccctt ctggtccttc gaaactttcg tttaccccat cgccttcttt
     4261 ttgtcggctc tactccttgg aaccgtaacc gtgggaatgc tactcggaca ctggtacctc
     4321 attgatacgg gtcagagtct ggatccattt atccgtatat acaaattttt cgtcggcgca
     4381 ctcgtcgtgc aaacgatctt tctactcatg tctgtgatcg cgcttcatat gttcggcgaa
     4441 tcggcaagtg ttatcggcct aagacgtttg tgggtggacc atcgcacact cttcctcaca
     4501 cgagtcatcg taggccaagc cgcccccttg atcttgtccg ggatgatctg gcgaaccttg
     4561 gtgattccga acaccatggc tgcgacaggt cttttttata tcgccctact gggggtcttc
     4621 gttggagaaa tcctgggaaa gcagatcttg gcactgacct cgctcccgtt ctagcaacgc
     4681 catagggaat gagttagccg acccgaggat aagtcttgat cttagtaatc cgcaaaccgc
     4741 tcgaagtcca tccaagatca tacccaatcc gttccccctc ccggagatcg agcggcgtct
     4801 ttgcctcacc ggagagaatg accagatcgt aggagaaccg tacctctcgc ccactctccg
     4861 tccgaaccac tcccatattg ttagatggga agagcttaac gatcacaccg tggtggtagt
     4921 aatcctctcc gaggggcggt gcaagattat cctcgttatc actcatgatt tagtttttag
     4981 ggctcctatg gattgagcac cgagccgcgc cacataaaac aatacctctt gccgcctcaa
     5041 tcagtaggaa tttggacgcg cgcacgaccg atgatcataa ccgcatacga cggaaaaaac
     5101 cgcggtggaa gaaacactaa ctcgttaatt gcttttgtct cgcgattcgc ccctgcaatt
     5161 ccatcaagcc gtccaaaacc gcctcgggtc gtggaggaca tccggggacg tacacgtcga
     5221 ctggaatgat acgatcaatt ccctgaacgg tcgcgtagtt atcgtaaaag ccgccgctcg
     5281 ccgcacaagc gccaaatgcc atcacccact tgggctcgga catttgctca taaacacgct
     5341 tcagaaccgg ggaaagttta tgatttaccg ttcctataac catcaatagg tccgcctgtc
     5401 tcgggctaaa ccgcggcaac agcgcaccga aacgatcgat atcgtacgga gacatcgata
     5461 gcgacatgta ctccatcgcg cagcaagcgg tcgcgaacgg gtagggaaat agtgaatatt
     5521 tccgagccca accaaccgcc ttgtccaatc gagtcagcgc aacagaatcc gctaggagct
     5581 tatctgggcc catttttgat tcaatcgagg agttcgctga ttgcataagc acctgtcgcc
     5641 agtcgatttc ggcccgctga ga
//
LOCUS       DASSWZ010000312         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_380139, whole genome shotgun sequence.
ACCESSION   DASSWZ010000312 DASSWZ010000000
VERSION     DASSWZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380139"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFN58_07525"
     CDS             <1..411
                     /locus_tag="VFN58_07525"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HET8917875.1"
                     /translation="RGFQAVLLGACLLVAWDLLLDPAMSRVTSYWIWGEKGSYYGMPW
                     TNLAGWAITGFILLAMLRKLAPEPKGDLRFSVLVYFVNFLLPLGFCVLNGYWIAVFAG
                     IATVVAAFLFFGSGKASRLHQTASPELYGQPRTF"
     gene            628..>2229
                     /gene="dxs"
                     /locus_tag="VFN58_07530"
     CDS             628..>2229
                     /gene="dxs"
                     /locus_tag="VFN58_07530"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET8917876.1"
                     /translation="MTRLLDAIDTPKDLRRLSVAELSMLATEVREEVISIVSEVGGHF
                     ASTLGAVELTLALHYVFDTPEDRIVWDTGHQAYAHKLICGRRTRLATIRQLDGLSGFL
                     SREESEYDVFGAGHAGTSISAALGMVEANSLAGPPRKVVAVISDGGLSAGLTFEGLNQ
                     AGHLDKDLIVVLNDNAIFIDPRVGAVSSFLSKQFTTDFGVRIQKQLSSWLLSLPRGQD
                     LKHAARKLKDSFLGLVTPGFLFESLGFQYVGPIDGHNITEMVTTLQNLKKVDGPTLVH
                     VLTTKGKGYAPAESDPVKYHAVTPFHVLTGKAKKEKGPIPTYTDIFAQALIRLAKENP
                     RVVGITAAMGSGTGIDKLSREIPGRSYDVGIAEQHAVTFAAGMATEGFIPVVAIYSTF
                     LQRGYDQILHDVCLQNLHVVFALDRGGLVGADGPTHHGVFDFSYMRSIPNIVIMAPKD
                     ENELQHMLKTAVEHTGPISLRYPRGEGWGIPMDSQMATLEIGKAELLRDGSDVVIAAI
                     GQTVIPALKAAQDLAALGMDAAVVNA"
BASE COUNT          482 a          552 c          650 g          545 t
ORIGIN      
        1 cgtggatttc aggcggtgct gttgggagca tgccttttag tcgcctggga cctgttgctg
       61 gatccggcca tgagcagagt aaccagttac tggatttggg gtgaaaaggg gagctactac
      121 gggatgccgt ggacgaactt ggcaggttgg gcaattaccg gttttatact gctggcgatg
      181 ttgcggaagc ttgcgcccga accgaaaggc gatttacgat tttcggtttt ggtgtatttc
      241 gtcaattttc ttctgcccct gggattctgc gtgctgaatg gctattggat cgcagtcttc
      301 gcaggcatcg ccaccgtagt ggcagcgttc ctgttttttg gttccgggaa ggcgtcccgc
      361 ctgcatcaga ccgcatcgcc ggaactatat ggccaacccc gcacttttta ggggtccgtc
      421 ctggaaaggc tttttctata gaccggttgc atttgaagat gagattcagt caatgctaca
      481 ttagcggacg gcaaaagagc cacgacggag gttatacgga gttggccttg cttcagcggc
      541 aaaatcgttt gtgctcagct gccgcttggt tttgctcgcg cgtacatggt gtaacaaatt
      601 cagtcacgca ggagatgaat ctataaaatg acacgactgt tggatgctat cgataccccg
      661 aaggatctcc gccggctcag tgtcgcagag ctctctatgc tagcaacgga ggtgagagaa
      721 gaggtgattt ccatcgtgtc cgaagtggga ggacatttcg catcgactct cggtgctgtg
      781 gagctgacgc tcgccctcca ttatgtcttc gacacaccgg aagatcgcat cgtctgggat
      841 acgggacatc aggcgtacgc tcataagctg atctgtggcc ggcggacgcg gttggctact
      901 attcgccaat tggacggctt gagcgggttt ttgagccgtg aagagagcga atacgatgtc
      961 tttggcgccg gccatgcagg cacttctatt tccgcggcgc tcggcatggt ggaggccaat
     1021 tcacttgcag gaccaccgcg caaagtggtt gctgttatta gcgatggtgg cttgagcgcg
     1081 ggtcttacgt ttgaagggct caatcaggcc ggccatctcg acaaagatct aatcgtggta
     1141 cttaatgaca atgcgatttt catcgacccc cgcgtcggtg ccgtttcatc cttcctgagc
     1201 aaacagttca cgacggattt cggtgttcgg attcagaagc agctctcgag ttggctcttg
     1261 agtctcccca gaggacaaga tcttaaacac gcggcgcgca aattgaaaga ctccttctta
     1321 ggcctggtga cgccagggtt tcttttcgaa agcttgggct tccaatacgt cggtcccatc
     1381 gacggtcaca acataacaga aatggtcaca actttgcaga acctcaagaa agttgacggt
     1441 ccgaccttgg tacatgtgct gaccacaaaa ggcaagggtt acgcaccggc ggagagtgat
     1501 ccggtcaaat atcatgcggt caccccattt catgtgctca ccggcaaggc caagaaggag
     1561 aaagggccga ttccaaccta tacggatatt tttgctcagg ctctgattcg cttggcgaag
     1621 gagaaccccc gggtagtggg tattacggca gccatgggaa gtggtaccgg aattgacaaa
     1681 ctgtcacggg agattccggg ccggagttac gatgtcggaa tcgcagaaca gcatgcggtg
     1741 acattcgcgg ccggtatggc gactgaagga tttatcccgg tggtggcgat ctactccaca
     1801 ttcttgcagc gcggctacga tcaaattctt catgacgttt gcctacagaa cttgcacgtc
     1861 gtgttcgctc tggaccgggg tggtctggtg ggggcggacg gtcccactca tcacggtgtc
     1921 tttgattttt cttacatgcg atcgattcca aacattgtga ttatggcccc gaaggacgaa
     1981 aacgagttgc agcatatgtt gaaaacggca gttgagcaca cgggtcccat ttcacttcgt
     2041 tatccccgcg gcgaaggttg gggcatccca atggactctc agatggccac attggaaatc
     2101 ggtaaagcgg agctgctacg cgatggcagt gatgtggtga tcgccgctat cggccaaacc
     2161 gtcattcctg ccctcaaggc cgcccaagac ttggcggcgt taggcatgga tgcagcggtt
     2221 gtgaacgca
//
LOCUS       DASSWZ010000313         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_493632, whole genome shotgun sequence.
ACCESSION   DASSWZ010000313 DASSWZ010000000
VERSION     DASSWZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493632"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VFN58_07535"
     CDS             <1..978
                     /locus_tag="VFN58_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917877.1"
                     /translation="GYADSSLKDVLPVELDGKGSYQAQGPVRPVLTPSGKAHPVTRLL
                     PDPKGNEEAWAKMPALSALNQVRSARGETLLSAGSNGSGATAPLLTIGRFGKGRSLAL
                     MSDETWRWNFIAVGNRETPQNHLKLIRQAIRWLAQERSFEQVQIRPIPISRPGEKIGI
                     KLRVLKDDFTPTRQAAVQLRVIGPEGEPSLVQTTADAEEGEYTGEYTPTREGSYRAEA
                     EASLAGKTLGKDSASFSVAFPYAETDDGRPRPELLKQIATASQGEYIPIKDWNEKTLD
                     RIAAKLESHAPSQIIEQRQTRLWSTLWPFAIILALLSVEWWMRRKWGLI"
     gene            1051..1848
                     /locus_tag="VFN58_07540"
     CDS             1051..1848
                     /locus_tag="VFN58_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET8917878.1"
                     /translation="MTNLKLRAQYFAFLILPLAFPTRVFSFCHELEVVKRPFLRIAAT
                     APAPIEVEWMGHSTFQITSSKGTRILTDPHGAFDLPRPTLPQHIVTTSHQHGPHNSVH
                     MAPGTPVILHGLTPGGENWQKVSTTIRDVSVYLVPAYHDKSQGMQRGKNAIFVFRVDD
                     ICIAHLGDLGHVLTPPQLKLLGKIDVLLVPVAGGMFTVTPDEAVEVTKQINPKIAIPE
                     HYWWPGGADRFAAGFSKVKRLNGLVLKISKQELPPPIEIVVFAGTGR"
     gene            1941..2909
                     /locus_tag="VFN58_07545"
     CDS             1941..2909
                     /locus_tag="VFN58_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET8917879.1"
                     /translation="MKAVRVHKYGGPEVLTLEEIPVPEPKAGEARVKIEAIGVNYIDV
                     YQRTGLYPLQTPFTLGTEGAGIVDAVGPNVTEVKKGERVGYAMIPGSYAEYAIVPAAR
                     LVPIPPNIDARTAAALMTQGMTAHYLTHSTYPLKKGETALLHAAAGGVGLLLIQIAKQ
                     LGATVIGTVSTEAKAKLAKEMGADHLILYTQSDFLAEVKKLTDGRGVNVVYDSVGQTT
                     FDKSLDCLRPRGYLVLFGQSSGPVPPFDPGKLAAKGSLFLTRPSLPHYTLERSELLQR
                     ANDVFNWTATGKLKVRIDKTFPLAEVAEAHRQLEGRKTTGKVILLP"
     gene            complement(2920..>3409)
                     /locus_tag="VFN58_07550"
     CDS             complement(2920..>3409)
                     /locus_tag="VFN58_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET8917880.1"
                     /translation="ALEDAKPLANQQFAQGLDDLGRELREWFGRWGDYFGSGTMKSEE
                     RPLIAFILRNREKLDLSAAQVKSLEQLKNDFQKESIRRDADLRVAEMDLNGLLVADKV
                     DMAKVEAKVREIERLRSDLRIARIRTIQKGKEVLTADQRKRLQELLAEQQIAGFQPRS
                     VY"
BASE COUNT          796 a          964 c          952 g          697 t
ORIGIN      
        1 ggttatgcgg acagcagcct gaaggacgtg cttccggtgg agctcgacgg caaaggaagt
       61 taccaagctc agggtccagt gcgtccggtt ctgaccccat ccggcaaagc ccatccggtc
      121 acccgcttgt tgccggatcc gaagggcaac gaagaggcgt gggcaaaaat gccggcgctt
      181 tccgccctga accaggtccg cagcgccaga ggggaaacgc tcctatcggc gggaagcaac
      241 ggctcgggcg ctacggcgcc actgcttacc atcggccgtt ttggcaaggg acgcagtctc
      301 gctttaatga gcgatgaaac ctggcgttgg aattttatcg ccgtcggcaa tcgggaaact
      361 ccgcagaacc atttaaagct cattcgccag gccatacgtt ggctcgctca ggaacggtct
      421 tttgaacagg tgcagatccg gcccataccg atctctagac ctggcgaaaa aatcggcatc
      481 aaactccgcg tactaaagga tgacttcacc ccgacccggc aagccgctgt gcaactgcgc
      541 gtcatcggcc cggaaggcga gcctagcctg gtgcaaacaa ctgcggatgc agaagaaggt
      601 gaatataccg gcgagtatac gccgaccagg gaaggctcct atcgcgcgga agcggaagcc
      661 agcctggcgg gaaaaacttt gggcaaggac agtgcaagct tttccgtcgc ctttccctac
      721 gccgagaccg acgacggcag gccccgtccg gagctgctga agcagattgc cacagccagt
      781 cagggagagt atatcccgat caaagactgg aacgaaaaaa ctctcgatag aatcgcggcg
      841 aaactggaga gccacgcgcc gtcgcagatt atcgagcagc ggcaaacgcg gctctggagc
      901 acgctttggc ccttcgccat catcttggcg cttttgagcg tcgaatggtg gatgcggcgg
      961 aagtggggct tgatctagtt gcgggtttca ggtttcagtt aacgcgagac tcccaagacg
     1021 aaactccaac tggtgaggat acgcaaccct gtgacaaacc taaaactaag agcgcagtac
     1081 ttcgcgtttc tgattttgcc gctggctttc ccgacccgcg ttttcagctt ctgtcacgaa
     1141 ctggaggtcg taaagcgtcc attcctgcgg attgcggcca ctgctccggc accgattgaa
     1201 gtggaatgga tgggccactc gacctttcaa atcacctcgt ccaagggaac tcgaattctt
     1261 accgacccgc acggcgcctt cgacctgccc cgtcccactc tgccacagca catcgtcacc
     1321 accagccatc agcatggacc gcacaacagc gtccatatgg ctcctggaac accggtcatt
     1381 ctgcatggac tgacgcccgg tggggagaat tggcagaagg tctccaccac catccgggac
     1441 gtttctgttt acctagttcc cgcgtaccat gataagagcc aaggcatgca gagaggtaag
     1501 aatgcgatct tcgtctttcg ggtcgatgac atctgcatcg cccatctcgg cgacttgggc
     1561 cacgtgctta cgccgccgca gcttaagttg ttggggaaaa tcgatgtcct gctggtgccg
     1621 gtcgccggcg gtatgtttac ggttactccc gacgaggcgg tggaggtgac gaaacagata
     1681 aatccgaaaa tagcgatacc tgagcactac tggtggccgg gaggcgcgga tcgattcgcc
     1741 gcgggattct caaaagtgaa acgactgaat ggactcgtcc taaagatatc caagcaggaa
     1801 ttaccgccgc cgattgaaat cgtcgtcttc gcgggaaccg gccgctgaag cggaaggatt
     1861 gaattcagga gccagaagat ttcgagcccg ggttctggtt ccatagcgac ataacctgga
     1921 gcttcaaagg aggagagagt atgaaagcag tgcgagttca taaatatggc ggtccggaag
     1981 ttttgaccct cgaagagatc cctgttcccg aacctaaagc cggtgaggcc cgtgtcaaaa
     2041 tcgaagctat cggcgtgaat tatatcgatg tgtaccagcg gacagggctc tatccgctgc
     2101 agactccctt tacattggga acggaagggg cggggatcgt cgacgcagtc ggacccaatg
     2161 tcacagaagt gaaaaaaggg gagcgtgtgg gctacgcgat gattccggga tcttacgctg
     2221 aatacgccat cgtacccgca gctagacttg taccgatacc ccccaacata gacgcgagga
     2281 cagccgccgc tcttatgacc caggggatga ccgcccatta cttgacccac agcacttatc
     2341 cactgaagaa aggcgagacg gcactcctgc acgccgcggc cggcggggtg ggactcttgc
     2401 tgattcaaat cgccaagcag ttgggtgcaa cggttatcgg cacggtctcg accgaagcca
     2461 aagccaaact cgcgaaagaa atgggtgccg accatttgat tctttatacc caaagcgact
     2521 ttctcgctga ggtcaagaaa ctcacagatg gccgcggagt gaacgtcgtt tatgactcgg
     2581 tgggacagac tacgttcgat aagagcttgg attgcctccg accaaggggt tacctggtcc
     2641 ttttcggaca atccagcgga cctgtgccgc cttttgatcc cggcaagctt gccgccaagg
     2701 gctctctatt tctgactcgc ccgagcttgc ctcactatac gttggagcgt tcggagttgc
     2761 ttcaaagagc aaacgatgtc ttcaactgga ccgctacagg gaagttaaaa gtgagaatag
     2821 ataagacctt cccgctggcc gaggtggcgg aagcccaccg ccagctggaa ggacggaaaa
     2881 ctacaggtaa agtgatttta ctgccgtaaa atctttaacc taataaaccg acctgggctg
     2941 gaaacctgcg atctgctgtt ccgccagcag ttcctgcaac cttttgcgct gatcggcggt
     3001 aagcacctct tttcccttct gaatggttcg aatgcgcgca atccggagat ctgagcgcaa
     3061 gcgctcgatc tcccgtactt ttgcttcgac cttcgccata tcgactttgt ctgccacgag
     3121 aaggccattc agatccatct cggcaacgcg aaggtcggca tctctccgaa tcgattcttt
     3181 ctggaagtcg ttcttcaatt gctcaagact tttgacctgg gcggcggaga ggtcgagctt
     3241 ctcgcgattg cggaggataa aagcgatcag cggccgctcc tcgctcttca tggtgccgga
     3301 accgaaatag tctccccagc gaccgaacca ttcacgcaac tcgcgcccaa gatcatccaa
     3361 accttgcgcg aactgctggt tggccaacgg ctttgcgtct tcaagcgca
//
LOCUS       DASSWZ010000314         7833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_847209, whole genome shotgun sequence.
ACCESSION   DASSWZ010000314 DASSWZ010000000
VERSION     DASSWZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847209"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFN58_07555"
     CDS             complement(<1..536)
                     /locus_tag="VFN58_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET8917881.1"
                     /translation="MFDKFHEECAVMGVYGHPEAANMVYLGLYALQHRGQESCGIVSS
                     DGRGLISHRQMGLVADVFREDVIKRLEGSRAIGHNRYSTTGQSHLKNAQPFVVEYSQG
                     PIAISHNGNLVNAEVLRKELEDSGSIFQSTSDTEVIIHLIATSKESTLMGRIVEALSR
                     TRGAYSLLFLTLDRMIAAR"
     gene            complement(548..2782)
                     /gene="purL"
                     /locus_tag="VFN58_07560"
     CDS             complement(548..2782)
                     /gene="purL"
                     /locus_tag="VFN58_07560"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET8917882.1"
                     /translation="MRDEKAAQFTQPLVTPEVVASHGLTPEEYRKIVKLLGREPNHTE
                     LGVFSVMWSEHCSYKSSRHYLKRLPTEGPQVLQGPGENAGVVDIGDGLAVAFKMESHN
                     HPSFIEPYQGAATGVGGILRDIFTMGARPIASLNSLRFGSIDHPRTRYLLSGVVAGIG
                     GYGNCIGVPTVGGEVYFDECYDANILVNAFTLGIAKKKRIFTGVARGVGNPVIYVGSK
                     TGRDGIHGATMASESFSEEKEQRRPTVQVGDPFTEKLLLEACLELMETDCIVGIQDMG
                     AAGLTSSSSEMAARGGNGIELDLSFVPLREQGMTPYEILLSESQERMLLVAKKGTEDE
                     VKRIFDKWDLDAVVIGRVTDDRQFRALFDGVEVVSIPISALTKEAPVYQRPVERPKLQ
                     DQLEQFDLSNLAEPADLGSTVKLLIGSPNIASKEWVYRQYDYFVRSNTVVAPGADAAV
                     IRLKGTEKGLALTVDGNSRYCYLDPYVGGALAVAEAARNLACVGARPLGLTDCLNFGS
                     PENPEVMWQFSRVIEGMRDACIALKIPVVSGNVSFYNETDGVPIYPTPTVGMVGVVGK
                     IARVVTPWFKAAGDVVVLLGRTREELGGSEYLKIVHGLVKGTPPWIDLRLEQAVQSCC
                     VAAIEKGILRSAHDISDGGIAIALAECCISGPEKPLGVRIEMREMIRGDALLFSESQS
                     RIVVSVAEENLEQLREVAGGYHVPMQVIGAVGGTSFVIQPLLRLPVDELRAIWSAGLT
                     SRIK"
     gene            complement(2775..3485)
                     /gene="purQ"
                     /locus_tag="VFN58_07565"
     CDS             complement(2775..3485)
                     /gene="purQ"
                     /locus_tag="VFN58_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET8917883.1"
                     /translation="MRWGVIVFPGSCDDQDVFDTLKRDMGQKVKFLWHKDDLAGHFDC
                     LVLPGGFSYGDYLRCGAVARFSPIMRGVLEFAGQGGLVLGICNGFQILCESGLLPGAL
                     VRNAGLQFVCQQVVLRVEETDTPFTCEAKKGQLLKLPVKHGEGCYYADPATLERLRKN
                     HQILFRYVDAKGRASPSANPNGSLDNIAGVCNEGRNVFGLMPHPEDACNKLLGSEDGR
                     KIFSSITAACAQREHRHA"
     gene            complement(3489..3731)
                     /gene="purS"
                     /locus_tag="VFN58_07570"
     CDS             complement(3489..3731)
                     /gene="purS"
                     /locus_tag="VFN58_07570"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET8917884.1"
                     /translation="MRVKIFVSLKGGVLDPQGKAIEGSLHTLGYAEVQDVRMGKYLEF
                     NVEASSREAAESRIREMCEKLLANPVIEDYRFEIEG"
     gene            complement(3764..5056)
                     /gene="purB"
                     /locus_tag="VFN58_07575"
     CDS             complement(3764..5056)
                     /gene="purB"
                     /locus_tag="VFN58_07575"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET8917885.1"
                     /translation="MIARYTRPEMGRIWSEENGFQKWLDVEILAAEGLAQLGQVPKAA
                     VARIKKKARFDVARIRKIESEVKHEIIAFLSCVAESVGDDARFLHVGMTSSDVMDTAL
                     AIQFKEASAILLQDVKDLMKVLRRQAFKYKWTVMIGRTHGVHAEPITFGLKLALWYQE
                     MARNLARLEKAVDELCVGQISGAVGTFAQISPKVEAYVCRKAGLKPALISNQIIQRDR
                     HAYFFATLAVIASSLEKFAVEVRHLQRTEVQEAEEPFTEGQKGSSAMPHKRNPILSEN
                     VSGLARLMRGYAMAALENVPLWHERDISHSSVERVIAPDATIVLDFMLRRMTYVLGNL
                     CVYPENMKRNLEKSGGTVFSEKVLLALVDKGIPRDSAYRMVQHHALKVGREGGDLKAE
                     LLQDPEIRRTLTAQEIEKVWGIKQHLTNIDFIFRRVFN"
     gene            complement(5107..5625)
                     /gene="dut"
                     /locus_tag="VFN58_07580"
     CDS             complement(5107..5625)
                     /gene="dut"
                     /locus_tag="VFN58_07580"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET8917886.1"
                     /translation="MEEMRIQIKRLRPSSAEITLPVYMTEGSAGMDLCADIDGELRLA
                     PMERALIPTGIAIALPPGLEAQIRPRSGLALKQGLTMVNSPGTIDSDYRGEVQLIVIN
                     LGQEPVIIQRGQHIAQMVVQRIIRARWQEVKELPLSGRQDGGFGHTDENNTVQRSKFK
                     RFKGRTKHASKS"
     gene            complement(5677..6939)
                     /locus_tag="VFN58_07585"
     CDS             complement(5677..6939)
                     /locus_tag="VFN58_07585"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET8917887.1"
                     /translation="MFAKTVLDNGFRIVTQEMLDHRSVSLGIWVENGSRHEAAHENGI
                     SHFIEHLLFKGTEQRTAAQIAEEMDAVGGVLNAFTSKEHTCYYAKVLDEDLPLAIDLL
                     TDIFLHSNFDGDEIERERSVILQEISQAEDTPDDYIHDLFSVDFFKDHPLARPICGSL
                     ETVNSFRRQDFLSFFKSRYRPRRVVVAGAGHLRHDQLVQAFKERLGSVSDSVYDGRAS
                     SQDGAGVPEMRSGIFQHAKSLEQVHLCLGVPGVHQTHPQRYTAYVLNTLLGGGMSSRL
                     FQEIREKRGKAYSVYSFSSAYKDVGYLGVYAGTSLEWVEEVVELILKELKQLAAGDIK
                     DEEIRRTQGQLVGSMMLGLESTDSWMSHIARNEIYFGKPITTDEICREVRAVSRDAVV
                     DLASALFGSQGIALSLLGDFKDAIKLKI"
     gene            complement(6963..>7833)
                     /locus_tag="VFN58_07590"
     CDS             complement(6963..>7833)
                     /locus_tag="VFN58_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET8917888.1"
                     /translation="YTVRIVSEVLESNGSTSMATVCGGSLSLMDAGVPVSAPVAGIAM
                     GLIKEGEHVRVLSDILGDEDHLGDMDFKVAGTPNGVTSLQMDIKISGVNRDIMRQALY
                     QAREGRLHILGIMTETMGSHRASVSGHAPRITTLKVRPDKIREIIGPGGKVIRGIIEA
                     TGVKMDVEDDGTVTIASSDEAASRKAIEMVQRIAAEAEVGKIYKGTVRKIVDFGAFVE
                     ILPGTDGLVHISQLAPERVRRVSDVLKEGDEVLVKVLEIDKQGKIRLSRKEALQETGN
                     GSSGSDPDDGPQK"
BASE COUNT         1762 a         2380 c         1975 g         1716 t
ORIGIN      
        1 cgggccgcga tcatccgatc taaagtcaaa aacagcaggg aataggcgcc gcgcgttcgg
       61 ctcagagcct cgacgatgcg ccccatgagc gtcgactctt tcgatgtcgc aatcaagtga
      121 atgatgactt cagtatcgga ggtggactgg aagatggaac ccgagtcctc caactctttt
      181 cgcaatactt cagcgttgac cagattgccg ttgtgagaga tcgcgatggg cccttgagaa
      241 tattcgacga caaagggctg ggcatttttc agatgacttt ggccggtagt ggagtagcga
      301 ttgtggccga tggccctcga gccctccaac cgcttgatga cgtcctctct gaatacatcg
      361 gcgaccagac ccatctgccg atgggaaata aggcctctgc cgtccgaaga aacaatgccg
      421 cacgattcct ggccgcgatg ctggagggca taaagtccta aatagaccat gttggccgct
      481 tcgggatggc catagactcc catcaccgcg cattcttcgt gaaacttgtc gaacattttc
      541 gtagccccta tttgatccgg ctcgtcagcc ccgctgacca aatcgcgcgc agttcgtcta
      601 ccggcagccg cagcagaggc tgaattacga aacttgtacc tccaaccgct ccgataacct
      661 gcatcggcac gtgatacccg ccagcgactt cccgcagttg ctccagattc tcttccgcaa
      721 ctgatacgac gatccgcgat tgcgactcgc taaaaagcaa agcatctcca cgaatcattt
      781 cacgcatctc aatccgtacc cccaacggtt tttcagggcc gctgatgcaa cactctgcca
      841 acgcgatagc gatcccgcca tccgatatgt catgagccga ccggagaatg cccttctcaa
      901 tggctgcgac gcagcagctc tgaaccgctt gttccaaccg aagatcgatc catgggggag
      961 tccctttcac caacccatgg acaattttta ggtactcact gccacccagt tcctctcgcg
     1021 tccgcccaag caagacaact acatcgccag cggctttaaa ccatggggta acaaccctag
     1081 cgatcttgcc gacgacaccc accatcccca ccgtgggcgt gggatagata ggaactccat
     1141 cagtttcatt atagaaacta acgttgccgc tcacaacggg gatctttagc gcgatgcaag
     1201 catctcgcat cccctcgatg acccgggaaa attgccacat cacctccggg ttttccggac
     1261 tgccaaaatt aaggcagtcc gtgaggccga gcggtcgagc accaacgcaa gcgagattgc
     1321 gcgccgcttc cgcaacggct aaagcccctc ccacgtaggg atcgagatag cagtagcgac
     1381 tattgccatc gactgtcagt gcaagtccct tttccgttcc tttgagccgg atcaccgccg
     1441 catcggcgcc aggagctacc actgtattgc tgcgcacgaa ataatcgtat tgtcggtaaa
     1501 cccattcctt cgaggcgatg ttcggtgatc cgatgagaag cttcaccgtc gaacccaaat
     1561 ccgccggttc cgcgaggttg gagaggtcga attgctcgag ttggtcctga agcttcgggc
     1621 gctcaaccgg ccgctgatag accggcgcct ccttggtcaa agcgctgatt ggaatagaaa
     1681 ccacttcgac gccgtcaaaa agcgcgcgga actgccgatc gtccgtaacc ctgccaatca
     1741 caacggcatc gagatcccat ttgtcaaaaa tacgtttgac ctcatcctct gtgccctttt
     1801 ttgccaccaa gagcattcgc tcttgcgact cagagagcag aatctcgtag ggcgtcatgc
     1861 cctgctcgcg caagggcaca aaggaaagat ccagctctat gccgttgccg ccgcgtgccg
     1921 ccatttccga agaggagctc gtcagtccgg ccgcgcccat gtcctgaatc ccgacgatgc
     1981 agtccgtttc catcagctcc agacaagctt ccaagagcag tttctcagtg aacgggtccc
     2041 cgacctgaac ggtaggacgt cgctgttcct tttcttctga aaatgattcc gatgccatgg
     2101 ttgcgccgtg aattccgtcg cggccggtct tggaaccaac gtaaatcacc gggttgccga
     2161 cgcctcgcgc cacgccggtg aatattcgct tctttttggc gatacccaaa gtaaaagcat
     2221 tcaccaagat attggcgtca tagcattcgt cgaaataaac ttcgccaccc accgttggaa
     2281 caccgatgca atttccatag ccgccgatac ccgcaacgac tcccgatagt agatagcggg
     2341 tgcgcggatg atcgatgctg ccgaaacgga gcgagttcag agacgcgatc ggccgagcgc
     2401 ccatggtaaa aatgtcacgg agaatcccgc cgacgccggt ggccgcgccc tgatagggct
     2461 cgatgaacga aggatgattg tggctttcca tcttaaaagc caccgccaat ccgtcgccaa
     2521 tatcgaccac gcccgcattc tcgccgggcc cctgcagaac ctgcgggcct tccgtcggca
     2581 agcgtttcag atagtgtcgc gagctcttgt agctacagtg ctccgaccac ataacggaga
     2641 acactccaag ttccgtatga tttggctctc gcccgaggag cttgacgatc ttccgatatt
     2701 cctccggagt cagaccgtga ctggcgacaa cctccggggt cacaagcggt tgagtgaatt
     2761 gcgccgcttt ctcgtcacgc atggcgatgc tccctttgag cacacgccgc cgtaatcgac
     2821 gaaaagattt tgcggccatc ctcgctcccg aggagtttgt tgcaggcatc ttccggatga
     2881 ggcatcaggc cgaaaacgtt ccgcccctca ttgcacacac cggcgatgtt atccagcgag
     2941 ccattgggat tagccgacgg cgaagccctc cccttggcat cgacataacg aaacaggatc
     3001 tgatggttct tgcgcagccg ctccaaagtt gccgggtcgg cgtagtaaca accctcgccg
     3061 tgcttgaccg ggagtttgag cagctgtcct tttttagcct cgcaggtaaa cggcgtgtcc
     3121 gtttcttcga ctcgaagcac tacctgctgg caaacaaatt gaagcccggc atttcgcacc
     3181 agcgcacccg gcagcagacc cgattcacag agaatctgga agccattgca gattcccagc
     3241 accaggccgc cctgcccagc gaactcgaga acgccgcgca tgatcggcga aaaacgcgcc
     3301 acggcgccgc agcggagata gtcgccgtaa gaaaatccac cggggagcac caggcaatcg
     3361 aagtggcccg cgagatcatc cttatgccag agaaacttga ccttctgacc catgtcccgt
     3421 ttcaaagtat caaagacgtc ctggtcatcg caggaacccg gaaaaacaat tacgccccaa
     3481 cgcacggtct acccctcgat ctcaaaccga taatcctcaa tgactggatt cgccagcagt
     3541 ttctcgcaca tctcgcggat gcgggactcg gcggcttcac gtgaactcgc ttcgacattg
     3601 aattcgaggt atttccccat ccggacatcc tgcacctcag cataacccaa ggtatgcagc
     3661 gacccctcaa tggctttccc ttgggggtcc aacactccgc cttttagaga gacgaaaatc
     3721 ttaacgcgca tatcagatgc tttcagccgc cggctttatc acttcaatta aaaacccggc
     3781 ggaagataaa atcaatgttg gtaaggtgct gcttgatgcc ccagaccttc tcgatttctt
     3841 gagccgttag ggtgcggcga atctccggat cctgaagcag ttccgctttt agatcaccac
     3901 cctctcgtcc cactttcaag gcatggtgct gtaccatacg ataggcactg tcccgcggga
     3961 tccctttgtc gaccaaggcg agcagcactt tttccgaaaa aaccgtgccg ccactcttct
     4021 ccagattgcg cttcatgttt tccggataga cacagagatt gcccaatacg taagtcatgc
     4081 gacggagcat gaagtcgagc acgatcgtcg catccggagc aatcacccgt tccaccgaag
     4141 agtgactgat gtcccgctca tgccacaggg gaacgttctc cagcgccgcc atggcgtagc
     4201 cccgcatgag ccgggccaat cctgagacat tctctgagag gatcgggttt cgcttgtgcg
     4261 gcatcgccga cgagcccttc tgcccctcgg tgaagggctc ctccgcttct tgaacttcgg
     4321 tgcgttgcaa gtgacgaacc tcaacggcaa acttctccag cgagctcgcg atcaccgcca
     4381 aagtcgcaaa gaaataagcg tgacggtcgc gttggataat ttgattggag atcaacgccg
     4441 gctttagccc agcttttcga caaacgtagg cctccacttt tggcgagatt tgtgcgaacg
     4501 tacccacggc acccgaaatc tgaccgacgc agagttcatc tacggccttt tccagccggg
     4561 ccaggttgcg cgccatctct tgataccaga gcgccaactt caagccgaag gtaatcggct
     4621 cggcgtgcac accatgggtg cggccgatca tcacggtcca cttatacttg aaggcctggc
     4681 gccgtaacac tttcatcaga tccttcacgt cctggagcaa aatggccgaa gcctccttga
     4741 actgaatggc caaggccgtg tccatgacgt cggaggacgt cataccgaca tggaggaagc
     4801 gggcgtcgtc gccgacggat tccgccacgc aactcaggaa cgcgatgatc tcgtgtttaa
     4861 cttcgctctc gattttgcga atgcgcgcaa cgtcgaagcg agcctttttt ttaatccgcg
     4921 cgacggccgc tttaggcact tgccccagtt gagccaagcc ctcggcggca agaatttcca
     4981 catcgagcca cttctggaag ccgttctcct cggaccaaat acggcccatc tccggccgtg
     5041 tgtaccgcgc gatcatgccc tctaattaaa gctctggatg ttttaagggt tcaagacttc
     5101 aagggctcaa gatttggaag cgtgttttgt tcttcccttg aaccttttga actttgaacg
     5161 ttgaaccgtg ttattttcgt ctgtgtggcc gaacccgccg tcctggcgac ccgagagggg
     5221 taactctttg acctcctgcc accgcgcccg aataattcgc tgaacaacca tctgagcgat
     5281 gtgttggccg cgctgaatta tcaccggctc ttgcccgaga ttgatgacaa tcagctgaac
     5341 ctcaccccgg taatcggaat cgatggttcc aggactgtta accatcgtca atccttgttt
     5401 tagagctaac ccgctgcgcg gacgaatttg agcttctaag cccggcggta atgcaatggc
     5461 aataccggtc gggatcaacg cgcgttccat cggggccaac cggagctcgc cgtcaatatc
     5521 cgcacataga tccatgccgg cggaaccctc cgtcatataa accgggagtg tgatttcggc
     5581 cgaactcggg cgcagccgct taatctgaat tcgcatctct tccaccggct atctacacgg
     5641 cacgcccaag gaccggagaa tttagtttgc cctgctctag atttttagct tgatggcgtc
     5701 tttaaagtca cccagcagag atagcgctat tccctgcgag ccaaaaagcg cggaggcgag
     5761 atcaacgacc gcgtcacggg agacggcacg aacctcgcgg catatttcat ccgtggtgat
     5821 cggcttccca aaatagattt cattgcgcgc gatatggctc atccatgagt ccgtggactc
     5881 caggccgagc atcatgctgc cgacaagttg cccctgcgtc cggcgaattt cctcgtcttt
     5941 gatatcaccg gccgcgagct gcttcagttc cttgagaatc agctcgacca cttcttcaac
     6001 ccactcgagg ctggtgccag catagacacc caggtagcct acatccttat atgccgatga
     6061 gaacgaatag accgaataag ccttgccgcg cttttcgcgg atctcctgaa atagtcgaga
     6121 actcatgccg ccaccgagca acgtatttag cacataggct gtgtatcgct ggggatgggt
     6181 ttggtgcacc ccgggcacgc cgagacagag atggacctgc tcaagggatt tcgcatgttg
     6241 aaagatcccg ctccgcatct ccggcacgcc ggcaccgtct tgcgagctcg ctcggccgtc
     6301 gtagacggag tcgctcacag aacccagcct ttctttgaac gcctgaacca actggtcgtg
     6361 acgcaaatgg ccggcccccg caacaaccac acggcgcggc cgatatcgcg acttgaagaa
     6421 gcttaaaaaa tcctgccgtc gaaacgagtt gaccgtttcc aaactgccgc aaataggacg
     6481 ggccaaggga tgatctttga aaaagtcgac actaaaaaga tcatggatgt aatcgtccgg
     6541 cgtatcttcc gcttgtgaga tctcctggag gatgacggat ctttcacgtt cgatctcgtc
     6601 gccgtcgaaa ttagagtgga gaaaaatgtc ggtcaaaaga tcaatggcta acggtaggtc
     6661 ttcatccaaa acttttgcgt aatagcaggt atgttctttg gaggtgaagg cattcaaaac
     6721 cccacccacc gcgtccatct cttccgcgat ttgcgccgcc gtgcgctgtt cggtcccttt
     6781 gaacagaagg tgctcgatga agtgagagat cccgttctcg tgcgcggctt catggcgaga
     6841 accattctct acccagatcc ccagactcac cgaacggtga tccagcatct cttgggtaac
     6901 aattcggaac ccgttgtcaa ggacagtctt agcgaacatg aggaaaggat aggttcattg
     6961 ggttattttt gaggcccatc gtccggatcg cttccggacg aaccattgcc ggtttcctgg
     7021 agcgcctctt tacgactgag ccgaattttt ccttgtttat caatctccaa gactttcacg
     7081 aggacctcgt cgccttcttt caggacgtcg ctgacccggc gaacccgctc aggtgcgagc
     7141 tgtgagatat ggactaggcc atcggtaccc ggcaaaattt caacgaaagc gccgaaatcg
     7201 acaatctttc tcaccgtccc tttgtagatc ttcccgacct cggcctcggc cgcgatcctt
     7261 tgcaccatct cgatggcttt tctcgaagcc gcctcatcgc tggacgcaat ggtcacggtt
     7321 ccgtcgtctt ccacgtccat ttttacgccc gtggcttcaa tgatgccgcg aataacctta
     7381 ccgccgggtc cgataatttc acgaatttta tcgggcctga ccttcagggt agtaatccgc
     7441 ggggcatggc ccgagacact ggcccggtgc gatcccatag tctcggtcat gatgccgaga
     7501 atatgcaacc ggccttcccg cgcctggtac aacgcctgcc gcatgatgtc gcggttcaca
     7561 ccgctgatct tgatgtccat ctgcagcgaa gtgacgccgt tcggcgtacc ggcaactttg
     7621 aagtccatgt cgccgaggtg atcctcatca cccagaatat cggagagcac tcgcacatgt
     7681 tccccttctt tgatcagacc catggcgatc cccgccaccg gcgccgatac cggcacgccg
     7741 gcatccatca gggaaagcga tccgccacag accgtggcca tcgacgtaga gccattggac
     7801 tccaaaactt cagatacaat gcgcactgtg taa
//
LOCUS       DASSWZ010000315         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_437133, whole genome shotgun sequence.
ACCESSION   DASSWZ010000315 DASSWZ010000000
VERSION     DASSWZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437133"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /gene="rimI"
                     /locus_tag="VFN58_07595"
     CDS             <1..256
                     /gene="rimI"
                     /locus_tag="VFN58_07595"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET8917889.1"
                     /translation="VDIHNIAVHIEFRRQRIGQALLQQVLEQARSRDSSRVTLEVRFS
                     NIPAQKLYESVGFVSQGLRKGYYSDDGEDAVVMALELAPR"
     gene            636..1118
                     /locus_tag="VFN58_07600"
     CDS             636..1118
                     /locus_tag="VFN58_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET8917890.1"
                     /translation="MFKVGEKVVYPAHGVGEIEAIRSHVISGTERKFYMLRILETDMK
                     IMIPIDNVDSVGLRKVIDRAMVSKVYKVLRQKKVETDQQTWNRRYREYTEKIKTGSIL
                     EIAKVLRDLFVLKGDKELSFGERKMLDTARNLLVKELSIARSHSEEKIMEELRHIFTH
                     "
     gene            1206..1898
                     /gene="ispD"
                     /locus_tag="VFN58_07605"
     CDS             1206..1898
                     /gene="ispD"
                     /locus_tag="VFN58_07605"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET8917891.1"
                     /translation="MTGIIVAAGEGKRIGGPASKGFLPIAGRPLVLRTLDRFYSARSI
                     EKVILVIAGKELRQSQTLIQNDPNLGHRPWVLQTGGATRQESVRRGLEKLDSDCEIVA
                     IHDGARPFVSSSLIDRCVDEAYRVGAVVIGVPVRDTIKAVSEEHWVQATPARNSLWEI
                     QTPQVFRKEVIMEAHDWGVRQAIEATDDAMLVEGIGKPVFLLEGERTNIKITVPEDIL
                     LAEALLRDGRIH"
     gene            complement(1895..>2290)
                     /locus_tag="VFN58_07610"
     CDS             complement(1895..>2290)
                     /locus_tag="VFN58_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HET8917892.1"
                     /translation="VRHPLVEAGMTKEEIRGYSRELNLPTWDKPSSPCLSSRFPYGTE
                     INLERLKKIAACELLMKELGFREFRVRYHGDLARIEVAQGEFHRWFEKTTRDAVVQKF
                     KQIGFHYVSLDLQGYRTGSMNESLHPSAT"
BASE COUNT          577 a          554 c          609 g          550 t
ORIGIN      
        1 agtcgatatc cacaacatag ccgtccacat cgagttccgc cgtcaacgaa tcggacaagc
       61 ccttttgcag caggtgttag agcaggcgcg gagccgcgac tcatctcgag taacacttga
      121 agtaaggttt tcaaacatcc ccgcacaaaa gctatacgag tccgttggat ttgtgagcca
      181 gggactgcga aagggttatt actcagacga cggggaggat gccgtggtta tggcacttga
      241 actggctcct cggtgaagtg ctcttgtcaa taaccgggtg gtgaccaaag tagataggaa
      301 ttagatattt catatctaaa ctattgaaaa tgtaattgaa ttgcccgcgg cagtcctctt
      361 tgggcctctt gacgtaataa tttgccctgt tgtatagtca ttttaccctc tggcggtctg
      421 aaatacttgg ccgctaattt tttttggacc ttacgccagt ggaacggaac gaggaatgtt
      481 caatagactt tgcacccggc gccaattcag ctctcaggtt ttttccccat cgaccttaga
      541 aaatcaactc catactttta ttagtcagtt gtcagttcaa tatcgactgc agttcgaaaa
      601 tcatcgcgaa agcgatcggc cgcgaggagg tcactatgtt taaggttggt gaaaaagtcg
      661 tatatccggc ccatggcgtg ggcgaaatcg aagccatcag gagccacgtc atttccggga
      721 ccgagagaaa gttctatatg ttgaggattc tcgaaaccga tatgaagatc atgatcccca
      781 tcgataacgt ggattccgtc ggattgcgca aagttattga tcgcgcgatg gtctcgaaag
      841 tgtacaaagt tttgcgccaa aagaaagtcg aaacggatca gcaaacttgg aatcggcgct
      901 atagagaata tacggaaaaa atcaagacgg gctcgattct tgagatcgcc aaggtgttgc
      961 gcgacctttt tgtgttgaaa ggcgataagg aactctcttt cggcgagaga aagatgttgg
     1021 atacggcacg caatctgttg gtcaaagagc tttccattgc gcgctcccac tccgaagaaa
     1081 aaatcatgga agagctgcgc catattttca cccactgagt tcttgatcca ttcaaaattc
     1141 attggattta tcggagccga gagcaacctc tcggctcttt cgttttctcg cacaccaata
     1201 tgcatgtgac cggtatcata gtcgccgccg gcgaagggaa acggatcgga ggaccggctt
     1261 ctaaaggctt tcttcccatc gccggtcgtc cgttggtgtt acggaccctc gaccgattct
     1321 actccgctcg aagcatagaa aaagtgattc tagtgatcgc cggcaaagag cttcgacaga
     1381 gccaaacgtt gattcaaaac gaccccaatc tcggccatcg accatgggtt ttacaaaccg
     1441 ggggagccac ccggcaggaa tcggttaggc gcggactcga gaagcttgac tcggattgcg
     1501 aaatcgtggc gatccatgac ggggctcgac cctttgtctc gtcctcgctc atcgaccgct
     1561 gtgtcgatga ggcttatcga gtcggtgccg tggtcatcgg tgtgccggta agagacacca
     1621 tcaaggccgt ttcggaagag cattgggttc aagccacacc ggcaagaaat agtttatggg
     1681 aaatacaaac gccgcaggtc ttccgcaaag aagtcatcat ggaagctcac gattgggggg
     1741 tgcgccaagc gatcgaggcg accgatgacg cgatgctggt tgaggggatt gggaaaccgg
     1801 tttttctcct ggaaggagag aggacgaata ttaagattac cgtacctgag gatatcttgc
     1861 tggcagaggc gttattgcga gatggccgaa tccactaagt agccgacggg tgcaagctct
     1921 cgttcatact tccggtccga taaccttgca aatcgaggct cacgtagtga aaaccgatct
     1981 gcttgaattt ttgcaccacg gcatcgcgcg tggtcttttc gaaccatcgg tggaactcgc
     2041 cctgcgctac ttcgatccgg gccaaatcac catgatagcg gacacggaac tcccgaaagc
     2101 cgagttcctt catcaacagc tcgcaagcag cgattttttt taaccgttca aggttgattt
     2161 cggttccgta gggaaaacgg gaggagaggc aggggctgga cggcttgtcc caggtcggaa
     2221 gattgagctc gcgactgtaa ccgcgaattt cttccttggt cattccggct tccaccaagg
     2281 gatggcgaac
//
LOCUS       DASSWZ010000316         6411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_295549, whole genome shotgun sequence.
ACCESSION   DASSWZ010000316 DASSWZ010000000
VERSION     DASSWZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295549"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..857)
                     /locus_tag="VFN58_07615"
     CDS             complement(153..857)
                     /locus_tag="VFN58_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET8917893.1"
                     /translation="MSIVSELTATLDRMVNDQFESLEFKHLFSVPLTPERGRFYMIQN
                     ALYTANRRDCWGYVQGSAPLEVKRVIWQHESDELVNDPRANMDHYTLTVKQGEVLGLK
                     PEDFEKAELPAMVQACFHAWHYIAIRSHWLKAYTSSHMLERRNNGQIVKGGGMSFRMG
                     KKFENDLGINLKKMISLDLHAVADTEHSDNISEMFERYVKTIEDRELVLQGARDCMAI
                     DRAYRGALGYYMERLS"
     gene            complement(985..1701)
                     /locus_tag="VFN58_07620"
     CDS             complement(985..1701)
                     /locus_tag="VFN58_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET8917894.1"
                     /translation="MSEVANGIDRMWQRLVQMANDQFDSPSFKRLLNTNFSRQRAQQY
                     SIQMVYYVRNRRDCWGYVQGSAPLDVKRLIWEHEEDELIGRKREGKDDHITLAVKEGA
                     VLGLSAKAFDDTPELEGGIACFYSWLHLAQRPWLEAIASSAILEVRNSAELIQGGSLS
                     RRIGEKFERELGIPMKKQINNAEHVVMDLEHAHLLMRVARKYATTEAAQNAIIKGARE
                     SLIIDRVYRGHLADMLETVP"
     gene            complement(1848..3302)
                     /locus_tag="VFN58_07625"
     CDS             complement(1848..3302)
                     /locus_tag="VFN58_07625"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HET8917895.1"
                     /translation="MAITTHEHHGHDHDGAVGPHTHLTSVGIDIGSSTSHLMFSQLLI
                     GYPSLHQRRPMVLERRVIARSRILLTPFSGDWNIEAEPLKELVDATFQEAGLTRDDID
                     TGAVIITGEAARRDNARKIAELFSDEAGRFVCATAGPKLETIMAAHGSGAVLKSREEG
                     LTVLNIDVGGGTTKVSVIDDGRIRSTSALNIGARLLAHDEGEIMTRLEKAGRRFLADL
                     GHDLSIGAKVQEDLRRRLAARMAHVLFDAVAGGKPPWDDLYVSAPLGDLPRLDGILFS
                     GGVSEYIYGREASPFGDLGPYLGEEIRKEAEKRGFQILEASEGIRATVIGASQYTVQL
                     SGETIHVPDGANLPVRNLRVFVVHVDWESPVAARAETAVKKALSERDAEVRGAPFVLA
                     FSSPPFFGYGAVQELARGIDEAMASLPAPDRPDALVFEQNVGRVVGGILSSKWNLPCV
                     DEINLSELDFIDVGEVVEGEGFVPVVVKSLAFGV"
     gene            complement(3317..4393)
                     /locus_tag="VFN58_07630"
     CDS             complement(3317..4393)
                     /locus_tag="VFN58_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8917896.1"
                     /translation="MVMVEKEQHLKMRMEHIRFGKEELERRRKSPVHIPAEHVAAQLK
                     DHYHVYIVDPRLGFNQRTFRFWINYRALGEEMEYSGKQLGHRHTVEAVIYIHQGHGYS
                     VIDGVEYPWEAGDLICVPVFAWHRHHNESQEDLIYYAATTGPFSMATGIALYEEENYP
                     EYWVFAQKGEEAMKTLIPGGAEAPPGGKVDLDQSRWKPQGPRGNGEPTSAEIYFDQLN
                     FAQEEEKRRRAGKVLVKARDLKFQPTPMGRVAYGVDAKLGFHVKVLGTLFAEIPPGKR
                     SGAHRHIYEETNHIIAGEGYSIIEDQKYEWKKGDTLVIPVFAWHQHFNTGREAARFLV
                     HTNRPCMESTGYIHTQQGEPADYE"
     gene            complement(4528..5484)
                     /locus_tag="VFN58_07635"
     CDS             complement(4528..5484)
                     /locus_tag="VFN58_07635"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET8917897.1"
                     /translation="MMSDIQRPVWSFAAGYHLRAKPLCDGRVKMANFELKAVAFEEDG
                     KGHEEFLAGKYDTGEFSLANYLALKSRGEPFMAIPVFPNRKFRQSYIFVREDSPFKEP
                     AQLKGKRVGIPGWLNTAGLWARGILSDDYGVKAQDIHWIARRKTQLDVSVPASTQLDV
                     APGEGSLAARLLKGEFDAIIVPDFPDEDGVRRLFPDSKAVEQDFYRRTGIFPTSHAIT
                     FHKTYLDKHPSAAHEMFNACYEAKQLALRDDADATYSNFAWNRQVWEEQRAVMGPDPW
                     KYGIKGNEKVLNTIIRYAGEQGLLKNKVTIEDLFVPINEPQG"
     gene            complement(5538..>6411)
                     /locus_tag="VFN58_07640"
     CDS             complement(5538..>6411)
                     /locus_tag="VFN58_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917898.1"
                     /translation="ALERIRIGLSVRNVVFLPFYYAQEKKIFEKHGLDAELIQIRSDL
                     QVVGLVSGEIDFTPVIGPTVFAIANGMPLRAIAVLYKAPLFSLVSPATVTNIKSLEGK
                     QVAVSRLGSDSHRYGVMMMENSGVDPKKITFIQTGNTSVSLAAVQQGSVAAAVLSPPF
                     TGDMAEKGYKILARSRSLIELPWMGMSTSRQKIERQPEVVRNVLRSIREVAANIRRDK
                     AGVVGYIEKNFKVTPGVAAEAYEDINGVIIESLVMPEELLKSYLDKASSRGEIPKPLT
                     IGDAFDLSLMRSLK"
BASE COUNT         1387 a         1919 c         1570 g         1535 t
ORIGIN      
        1 acgcctatcc tggtgatcat gcttcaaagt ttataaattc tccgaattat agcaaaattg
       61 tcgcctgtat gaccagtttt ttctaagatc tctgaagagt cgcgacgctc atctaaaggt
      121 agccatgccc ctttgatgca atccgcaact ttctaactga gtctttccat gtaatagcca
      181 agcgcccctc gataagcccg atcaatagcc atgcaatcgc gcgcgccctg taaaaccaat
      241 tcgcggtcct cgatagtctt gacgtagcgt tcgaacattt cggagatatt gtccgagtgt
      301 tcggtatctg ccaccgcgtg tagatccagc gaaatcatct tcttgaggtt gatgccgagg
      361 tcattctcga atttcttacc catgcggaac gacatgccac cgccttttac gatttggcca
      421 ttgttgcggc gctccagcat gtgcgacgac gtataggcct taagccagtg gctacggatc
      481 gcgatgtaat gccaagcatg aaaacaggcc tgcaccatag ccggcagctc ggctttttca
      541 aaatcctccg gtttgagccc aagcacttcg ccctgtttca cggtcagtgt gtagtggtcc
      601 atattggccc gaggatcatt gaccagttcg tcgctctcat gctgccagat cactcgcttt
      661 acctcgagcg gagcagaacc ctggacgtaa ccccagcagt cgcgccggtt ggccgtatac
      721 agggcgtttt gaatcatata aaaacggcct ctctccggcg tgagtggcac ggaaaataaa
      781 tgcttaaact ccaacgattc aaactggtca ttgaccatcc ggtccaacgt cgctgtcagt
      841 tcactcacga tactcatact ccctcctaga gcaggttgct gaaaaagact catcgtaatt
      901 ttgaggatcg aagatagaga atggaagatg gcgattctcg atcctccatc tcacttctcg
      961 atcttgcgaa cgcgcgcctc gccatcaggg caccgtctcc agcatgtcag cgagatgacc
     1021 gcgataaacc cggtcgatga tcagactttc tctcgctccc ttgatgattg cgttctgggc
     1081 ggcttccgtc gtcgcgtact tccgcgcgac ccgcatcaag agatgggcat gctcgaggtc
     1141 cataacgaca tgctcggcgt tgttgatctg ctttttcatg ggtataccaa gttcccgctc
     1201 gaacttttct ccaatgcgcc gtgacagcga tcccccttgg atcaactcgg cggaattgcg
     1261 gacctccaaa atcgccgagg aagcgatggc ttccagccaa ggccgctgcg ccaaatgcag
     1321 ccatgaataa aagcaagcga tgccgccctc aagctcaggc gtatcgtcga acgccttagc
     1381 actcaatcca aggaccgcgc cctcttttac tgctaaggta atgtgatcgt ctttgccttc
     1441 acgtttccta ccgatcaact cgtcttcttc gtgttcccaa atcaaccgct tcacatccaa
     1501 aggcgctgat ccctggacgt agccccaaca gtcgcgccgg ttcctgacat aatagaccat
     1561 ctgaatcgaa tactgctggg cccgctggcg ggagaaattc gtattcagga ggcgcttgaa
     1621 cgacggcgaa tcgaattgat cgttggccat ctgaaccaat ctttgccaca ttctatcgat
     1681 gccgtttgca acttcgctca tagattttca ctatccaaaa gcttcccgta tttctagcgt
     1741 cctgtcccag aagttcacgg aagtccttcg tatatggctg atagcttatg gaatatagtt
     1801 gggacatgcg aactatacgc tatgagccat acgccataaa ccaactgcta cacgccaaac
     1861 gccaggctct tgactactac cggcacaaag ccctctcctt ccaccacctc acccacatcg
     1921 atgaagtcca attcggatag attgatctcg tcaacgcaag ggagattcca tttcgacgaa
     1981 agtatgccac ccacgactcg acccacgttc tgctcgaaaa ccagcgcatc cggtctgtct
     2041 ggtgccggca ggctcgccat ggcttcatcg atacccctgg ccaactcttg caccgcgcca
     2101 taaccgaaaa acggtggcga tgaaaacgcc aaaacaaacg gcgcgccacg tacttccgcg
     2161 tcccgttcgc taagagcctt cttgacggca gtttcggcgc gcgccgccac cggagattcc
     2221 cagtccacat ggaccacaaa aacccgaaga tttctaaccg gtagattcgc cccatcggga
     2281 acatgaatcg tttcaccgct cagctgaacc gtatattgtg aggcgccgat cacggtggcg
     2341 cgaatgcctt cactagcctc aaggatctgg aagccgcgtt tctcggcttc ctttctaatc
     2401 tcttcgccta agtaagggcc taagtcgcca aagggactcg cttcacgacc gtagatgtac
     2461 tcggagacac cgcccgagaa caggataccg tcaagccggg gcagatcgcc taagggcgca
     2521 ctgacataaa gatcgtccca cggcggcttc ccgccggcca ctgcatcaaa tagtacatgg
     2581 gccatgcggg cggccaaacg ccggcgcaaa tcttcctgga ctttcgcacc gatgctgaga
     2641 tcgtgcccaa ggtcggcgag aaaccgccgt ccggcttttt ccagccgggt catgatctca
     2701 ccctcgtcgt gcgctaacaa ccgggcacca atattgagtg cgctggtgct tcgaatcctg
     2761 ccatcgtcga tcacgctcac tttcgtcgtt ccgccgccaa cgtcaatgtt cagaaccgtc
     2821 aggccttctt cgcggctttt tagaactgct ccagagccat gggccgccat gatcgtctcc
     2881 aattttggcc cggcagtagc gcagacgaaa cggcccgctt cgtcagaaaa caactcagct
     2941 attttccgcg cattgtcgcg ccgcgctgcc tccccagtaa taataaccgc cccggtatcg
     3001 atatcgtccc gcgttaatcc cgcttcctga aacgtcgcat ccacgagttc cttgagaggc
     3061 tccgcctcga tgttccaatc gccgctaaac ggcgtgagca gaatccgcga gcgcgcaatc
     3121 acgcggcgtt ccaataccat gggccgccgc tgatgcagcg acggatagcc gatcagcagc
     3181 tgggaaaaca tcagatgcga agttgaagag ccgatgtcga tgccgacgct ggtgaggtgg
     3241 gtgtgcggcc ccaccgcgcc atcgtggtca tggccgtgat gttcgtgggt agtgatcgcc
     3301 atgattcggc tatctcttac tcgtagtcgg caggctcgcc ttgctgggta tggatatagc
     3361 cggtgctttc catgcaggga cggttggtgt gaaccaggaa gcgcgccgct tctctacccg
     3421 tattaaagtg ctggtgccag gcgaaaaccg gaatgaccaa tgtgtcccct ttcttccatt
     3481 cgtacttctg atcttcgatg atgctgtagc cctcacccgc aattatatga ttggtctctt
     3541 cgtagatgtg gcgatgggcg ccagatcgtt tgcccggtgg gatttcagcg aacaacgtgc
     3601 cgagaacttt cacatggaac ccgagctttg catcaacgcc gtacgcaact cttcccatcg
     3661 gcgtcggctg aaatttaagg tctctagctt tgaccaacac ttttcccgct cgccgccgtt
     3721 tttcttcctc ttgagcgaag ttgagctgat cgaaatagat ttcagccgac gtcggctcgc
     3781 cgttgcctcg cggtccttgc ggcttccacc ggctttgatc cagatcgact ttgccgcccg
     3841 gcggcgcttc cgcgccgccg ggtatcagcg tcttcatcgc ttcttcaccc ttctgggcga
     3901 agacccaata ctccggatag ttttcctctt cgtaaagtgc aatgcccgtc gccatggaaa
     3961 aggggcccgt ggtcgcagca taatagatca aatcttcttg actctcattg tggtgtcgat
     4021 gccaagcgaa cacgggcacg cagatgagat ccccggcctc ccaggggtat tcgacgccgt
     4081 cgatgacgct gtaaccgtga ccttgatgaa tgtagatcac cgcttccacc gtgtggcgat
     4141 gccctaattg cttgccgctg tattccatct cctcgcccag ggcgcgatag ttgatccaaa
     4201 aacgaaatgt tctttgattg aatcccagcc gcggatcgac gatatagacg tgatagtggt
     4261 ctttgagttg cgctgcgacg tgttcggccg gaatgtgaac gggagacttc cgtcgacgct
     4321 ccaattcctc tttgccgaag cggatatgct ccatccgcat cttcaagtgc tgttcttttt
     4381 ccaccatgac catgaatcgt ttcctcctcg acgctcctga atcgttccag ccgtttcaaa
     4441 cgttccaatc gttcaaaccg tgagcgacag attcgagatc cttttgccag ccttaaaaaa
     4501 ggctacgtga ctcgtcgcag tccgaactta gccctgcggc tcattgattg gcacaaatag
     4561 atcttcgatc gttaccttat tcttcagcaa accctgctcg ccggcgtatc gaataatcgt
     4621 attcaatacc ttttcattgc ctttgatacc gtatttccaa ggatcagggc ccatcactgc
     4681 tctctgctct tcccagacct ggcggttcca cgcaaagttt gaataagtcg catccgcgtc
     4741 atctcgcaat gccagctgct tggcttcata gcaagcattg aacatctcgt gcgccgccga
     4801 tggatgtttg tccaaataag tcttgtggaa ggtgatggcg tgactggttg ggaatatccc
     4861 ggtccggcga taaaaatcct gttccaccgc tttcgaatcg ggaaaaagcc gccgcactcc
     4921 gtcttcgtct ggaaagtccg gaacaatgat ggcgtcgaat tcccccttga gcaggcgcgc
     4981 cgccaacgaa ccttctcccg gcgcgacatc aagttgcgtg ctcgccggta cggacacatc
     5041 cagttgagtt tttcgtcgcg cgatccagtg gatgtcctgc gccttcacac cgtaatcgtc
     5101 gctgagaatc cctctcgccc acaatcccgc ggtattgagc caacctggaa tgcccacgcg
     5161 tttgcccttg agttgcgccg gttccttaaa cggcgaatcc tcgcgcacaa agatgtagga
     5221 ctggcgaaat ttgcggttgg ggaaaaccgg aatggccata aacggttcgc cgcggctctt
     5281 tagcgccaga tagttggcga gagaaaactc tccagtgtcg tacttgccgg ccagaaactc
     5341 ctcgtggccc ttgccgtcct cttcgaaggc aacggctttg agctcgaagt ttgccatctt
     5401 cacccggccg tcgcacagag gtttagctcg caggtgatag cccgcggcaa aggaccagac
     5461 cggtcgttgg atatcgctca tcattatctc tcctacgcca ggatgtataa aaacttctta
     5521 tcgccctgcg actacaatca cttcaaactt cgcatgagcg agagatcgaa ggcatcgccg
     5581 atggtcaacg gcttcggaat ctcgccgcgg ctgcttgcct tgtccaggta gctcttgagc
     5641 agctcctcgg gcatcaccag gctttcgatg atgacgccat tgatatcttc ataggcttcc
     5701 gcggcgacac cgggtgtcac tttaaaattc ttttcgatgt agcctacgac tcctgccttg
     5761 tcgcggcgaa tattggccgc cacctcgcgt atcgagcgca gcacgtttct taccacctcg
     5821 ggctgcctct cgatcttttg tctgctggtg gacattccca tccacggcag ttcgatgagt
     5881 gaccggctcc gggccaaaat cttataccct ttttcagcca tatcaccggt aaaaggagga
     5941 ctgaggaccg ccgcagccac ggagccctgt tgcacggccg ccaggctgac cgaggtgtta
     6001 ccggtttgaa tgaaagtaat tttcttcgga tccacgccgc tattttccat catcatgact
     6061 ccgtagcgat gactgtcgga tcctagccgc gacacggcaa cctgctttcc ttccaagctc
     6121 ttaatattgg tcaccgtcgc aggacttacc agcgagaaaa gcggcgcctt atacaatacc
     6181 gcgatggccc tgagcggcat accgttggcg atcgcaaata ccgtgggtcc gatcaccggc
     6241 gtgaaatcga tctcgccgga aactaacccg acgacctgca ggtcgcttct tatctgaatc
     6301 agctcggcgt cgaggccgtg cttctcgaaa attttctttt cctgcgcata gtagaacggc
     6361 agaaagacta cattgcgcac cgagagaccg atccgaatcc tttcgagcgc g
//
LOCUS       DASSWZ010000317         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1144745, whole genome shotgun sequence.
ACCESSION   DASSWZ010000317 DASSWZ010000000
VERSION     DASSWZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144745"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFN58_07645"
     CDS             <1..933
                     /locus_tag="VFN58_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET8917899.1"
                     /translation="SVIHAMGGEQDMTKMGGLRKYMPRTFITYLLATLAITGFPATAG
                     FFSKDGILEEAYARGGMGLWLAGLCGAGFTAFYMWRQVFMTFFGESRADEHTKHHLHE
                     SPGVMTWPLVILALGAFPVGLFGLPAFLGHHRFVEWLEPVFGHGHGEAAHLSHETAIL
                     LTVISVVVALAGFGLAYLMYYRQTLAPERFSSVAGGLFYRLSYNKYYVDEIYQAIFVN
                     GTLLLARIGSLFDQYVIDGIVDGSASLTRFISWLNGLFDNYIIDGMVNAVANITFWVG
                     NRLRRLQTGNINSYLYGILIAVVIAIIVKLRYWS"
     gene            956..2476
                     /locus_tag="VFN58_07650"
     CDS             956..2476
                     /locus_tag="VFN58_07650"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET8917900.1"
                     /translation="MEHVLTYMTFIPVAGMVVVLLLPSKSPNLIRWVSAAFTVPPLLM
                     ACWLYANFDPAKPGLQFVERAPWIPAYNIQYLVGVDGLSISMVLLTALLCFLCIFASW
                     GIDKGVKGYFALFLLLDAGMMGVFVALDFFLFFIFWEVMLLPMYFLIGVWGGPRREYA
                     AIKFFLYTLFGSGLMLLAILALYFTSEPHTFDMTVLISQANKFGTQTLSIWPFNQWGW
                     GFQHVVWMALFIGFAIKIPAFPFHTWLPDAHVEAPTAISVILAGILLKMGTYGILRIN
                     FPMLPAATADLAFWFLGALGAWNIIYGALVAMAQKDLKKLVAYSSVSHMGYVMLGMAT
                     FTTQGINGAVLQMFNHGTITGMLFLLVGVIYDRAHHRDIEGFGGLAAIMPIYTGVTAL
                     AFFASMGLPGLSGFISEILVLLGSWQKFPVLAIIGAGGVILTAGYLLWTIQRVYLGPL
                     NEKYKSLPEINGREMFTLVPLGAIVILVGVYPNVVLDLLRASLDQINQIVLPHLHS"
     gene            2479..>3891
                     /locus_tag="VFN58_07655"
     CDS             2479..>3891
                     /locus_tag="VFN58_07655"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET8917901.1"
                     /translation="MDLKNLESLSFFYPEMILSGTILLLIVVDLLVRSERTLATIAVI
                     GCVGSLVATFDLYSAQQGWLFHRMIILDNFSLFFKVFALVAAILTVWMSLGSNEIRRV
                     HQGEYYTLLLTCTLGIFFMASASNLLTAYLSLELVSLTSYVLTGFLPHNRRSSEAALK
                     YLIYGGVASGTMIYGMSWIYGMTGSLDYAAIQAALAQQQVSKVALFMAFVFILAGFGY
                     KMVFVPFHMWSPDVYEGAPTPFTAFLSVASNAAGAAIMIRFFFPGVSRMVAGGDWAFV
                     SGVEWPQVLLFVSMITMTVGNLCALNQRNLKRMLAYSGIAHAGYMLMGLAVLNNDGLQ
                     AILFYVVVYLIMNLGAFLVVVMIANATGNEEIESYRGLAWRGAVLPAACLAVFLFSLT
                     GLPPFAGFIGKFLLFAVAINEGGIFVILAVVAILNSVISLYYYAKIVKTMFLDTPDPG
                     DKSLTITANNFTLLIPLTALT"
BASE COUNT          682 a         1066 c         1071 g         1072 t
ORIGIN      
        1 agcgtcatcc acgcgatggg tggcgagcag gacatgacaa agatgggtgg cctcaggaag
       61 tatatgccgc gcacgttcat tacctatctg ctggcaaccc tcgcgataac gggctttccg
      121 gccactgcgg ggtttttctc aaaggatggc attctcgagg aggcttatgc ccgcggcgga
      181 atgggcctct ggctcgcggg actatgcggc gccggtttta ccgccttcta catgtggcgc
      241 caggtgttta tgacgttttt cggcgaaagc cgtgccgacg agcacaccaa gcaccatctg
      301 cacgagtcgc caggcgttat gacttggccg ctggtgatcc tcgccttggg ggcgtttccg
      361 gtcggtctgt tcggattgcc ggcgtttctt ggtcaccatc gcttcgtaga atggctggag
      421 cctgtcttcg gccatggcca cggcgaagcg gcgcaccttt cgcacgagac ggcgatactg
      481 cttacggtaa tatccgtcgt ggtggcgctg gcgggatttg ggttggctta cctgatgtac
      541 taccgtcaga ccttggctcc ggagcgcttc tcttctgtcg ccggtgggct gttctatcgg
      601 ctgtcctata acaagtatta cgtggatgag atctatcagg cgatctttgt taatggaacg
      661 ttgctgctgg cgcgcatcgg ctctctgttc gaccaatacg tcattgacgg gatcgtcgac
      721 ggctccgcca gcctgaccag atttatctca tggcttaacg gccttttcga taactacatc
      781 atcgacggta tggtgaatgc ggttgcgaac atcactttct gggttggcaa ccgcctccgc
      841 aggctgcaga cgggcaacat caatagctac ctttacggga ttttgatcgc ggtggtcatc
      901 gccatcatcg tcaagttgcg ttactggagt tgattctgac gggagaatgt aaacgatgga
      961 acacgtgctc acgtacatga cctttatccc agttgccggc atggttgtgg tgctgcttct
     1021 gccgagcaag agccctaacc tgatccgctg ggtttctgcc gcctttacgg ttcccccgct
     1081 gctgatggcg tgctggttgt acgcgaactt cgacccggct aagcccggtc tgcaatttgt
     1141 cgaaagagct ccatggattc ctgcttacaa cattcagtat ttggtcggtg tcgatggcct
     1201 cagcatctcc atggtgctgc ttaccgccct gttgtgcttt ctctgcattt tcgcctcctg
     1261 gggcatcgat aaaggagtca agggatactt cgcgcttttc ctattgttgg atgcgggtat
     1321 gatgggtgtc ttcgtggccc tcgatttttt cctctttttt atcttttggg aagtgatgct
     1381 tctgcccatg tactttctca tcggagtctg gggcggaccg cggcgggagt atgccgccat
     1441 caagttcttt ctttacaccc tttttggcag cggcttgatg ttgctcgcca ttctggcgct
     1501 gtactttacc agcgagcccc acactttcga tatgacggtg ctgatttctc aagccaacaa
     1561 attcggtact caaaccttgt ctatttggcc gttcaatcag tggggttggg gcttccagca
     1621 cgtggtttgg atggcgcttt tcatcgggtt tgcaataaaa attccggcct ttccgtttca
     1681 cacttggttg ccggacgccc acgtcgaagc gcccacggcg atctccgtca tcctggctgg
     1741 catattgctg aagatgggca cctacgggat cctgcggatc aattttccga tgctgccggc
     1801 ggcgacagcc gatctcgctt tctggttcct gggcgcatta ggcgcctgga acatcatcta
     1861 cggtgccttg gttgccatgg cacaaaagga tctcaaaaag ctggtagcct attccagcgt
     1921 cagccatatg ggttacgtca tgctcggtat ggcgaccttc acgacacagg ggatcaacgg
     1981 tgcggtgcta cagatgttta atcacggaac catcaccggc atgctctttc ttttggtggg
     2041 tgttatctat gaccgtgctc atcatcgtga catcgaaggg ttcgggggat tggccgcgat
     2101 catgcccatc tatacgggtg tcacggcgct cgccttcttc gcctccatgg gcttacccgg
     2161 tctctccgga ttcatctccg aaattctggt tttactcggg agttggcaga aatttcccgt
     2221 gctcgccatt atcggcgcgg gcggtgtcat cctgaccgca ggttacctcc tgtggacgat
     2281 tcagcgcgtt tacctgggtc cgctcaacga gaagtacaag tcgcttcccg agattaacgg
     2341 ccgggagatg ttcactctgg taccgttggg agccatcgtg attctcgtcg gtgtctatcc
     2401 gaatgttgtg cttgacctcc tgcgggcatc cttagaccag atcaaccaga tcgtcttgcc
     2461 ccatcttcac agttaaccat ggatctcaaa aacctcgaaa gcctgagctt tttttacccg
     2521 gagatgattc tttccggcac gatcctgttg ttgatcgtgg tggatctgct cgtccggagc
     2581 gagcggaccc tcgcgacaat tgccgtgatc ggttgcgtgg gatctcttgt ggcgactttc
     2641 gatctttata gcgcgcagca gggttggcta tttcaccgga tgatcatcct ggataacttc
     2701 agtctcttct tcaaagtctt cgccctggtg gcagcgatct tgaccgtctg gatgtcattg
     2761 ggtagcaacg agattcgccg ggtccatcaa ggggaatatt acacgctgct tttgacctgc
     2821 actttaggta tttttttcat ggcatcggcg agtaatctgt tgaccgccta tctttctctg
     2881 gagctggtca gcctgacgtc ttatgttttg accgggttct tgccgcataa ccgtcgttcc
     2941 agcgaggcgg ccctaaaata tcttatctac ggcggcgttg cgtccggtac gatgatctac
     3001 ggcatgagct ggatttacgg tatgaccggc agcctcgact acgccgcgat ccaggcggct
     3061 ctggcgcaac agcaagtgag caaagtcgcc ctgttcatgg cttttgtttt tattctcgcc
     3121 ggatttggct acaagatggt ctttgttcct tttcacatgt ggtcgcctga cgtttatgag
     3181 ggagcgccga cgcctttcac agcgtttctt tcggtggctt ccaatgcagc gggcgccgcg
     3241 atcatgattc ggtttttctt ccccggcgtc tcgcgcatgg tggcgggtgg cgattgggct
     3301 tttgtctcgg gggtcgaatg gccgcaagtg ctgttgttcg tgagcatgat caccatgacc
     3361 gtcggcaacc tctgcgccct gaatcagagg aatctcaagc ggatgctcgc ctattcgggc
     3421 atcgctcatg ccggctacat gctgatggga cttgcggtcc tgaacaatga cggtctgcag
     3481 gcgatccttt tctacgtcgt ggtctatctg atcatgaatc ttggcgcctt tctcgtggtg
     3541 gtgatgattg ccaacgccac cggtaacgaa gagatcgaga gctatcgagg gctcgcttgg
     3601 cgcggtgcgg tccttccagc ggcctgcctg gcggttttcc ttttctcttt gacgggactc
     3661 ccgcccttcg cgggcttcat cggcaaattc ctactcttcg ccgtagcgat caatgaaggc
     3721 gggatcttcg tcatcctggc cgttgtcgcc atcttgaaca gtgtcatatc gctctactat
     3781 tacgccaaga tcgtcaagac catgtttctc gacacgcccg accccggcga caaatcgtta
     3841 accatcacgg cgaataattt cacgctcctc atccctctaa cggcactcac g
//
LOCUS       DASSWZ010000318         7554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_12644, whole genome shotgun sequence.
ACCESSION   DASSWZ010000318 DASSWZ010000000
VERSION     DASSWZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12644"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1186
                     /locus_tag="VFN58_07660"
     CDS             209..1186
                     /locus_tag="VFN58_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HET8917902.1"
                     /translation="MAGLKLTMALSHYDRHIPFFDKSVQVEGVDLQILEVGQSEPLKH
                     GRDRHERMLQKREFDVCELSLSSYLMAKSRGMPFTAIPVFPRRLFSLSQMWINVDSGI
                     NSPRHLIGKRVGLSTFQTTLSVLAKGDLQSEYDVPWRKVEWIVSKEEAVPFKPQEGVH
                     IELVAPGKKMGAMLERGEISALMVPHPPEQALKGSPTIRRLFADPKQEELKYFRKNGY
                     YPIMHVVAFKDEVLAKNLWLAQSVMAAFAKAKEACMAYYDDPNWSRSVWGRHLFEEER
                     IAFGDDPWPHGVKKNRANLERFIGYSLDQGLMESKLEVADLFAATTLDS"
     gene            1197..1394
                     /locus_tag="VFN58_07665"
     CDS             1197..1394
                     /locus_tag="VFN58_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET8917903.1"
                     /translation="MSLRVKCPTCHRETEWENNPHRPFCSDRCQLIDLGGWTEERYRI
                     PGSETQLPLDDEDDDGDEKVR"
     gene            1436..2215
                     /gene="lpxA"
                     /locus_tag="VFN58_07670"
     CDS             1436..2215
                     /gene="lpxA"
                     /locus_tag="VFN58_07670"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET8917904.1"
                     /translation="MSIHPTAVIDPKAEIDSEVEIGPYVVIEGPVRIKRGTRVMAHAY
                     LAGWTEIGESNEIHPGVVLGEAPQDKAYKGEETYLRIGAHNVFREYVQVHRGSAPGSS
                     TTIGNENFLMACSHIGHNSKLGDQVILANNALLGGYVEVGNSVFISGNCVVHQFVRIG
                     DYALMRGLSGTSRDVPPYAIIDWQHTVRGVNVVGLKRAGFDDQRIREIREAFRILFRK
                     GRNLSLAIKEINSHGNINGDVLALLDFIRSSKRGVCGGASE"
     gene            complement(2247..5294)
                     /gene="glnE"
                     /locus_tag="VFN58_07675"
     CDS             complement(2247..5294)
                     /gene="glnE"
                     /locus_tag="VFN58_07675"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET8917905.1"
                     /translation="MEHWLVSQLSKILSSSVQRKKLERLLKESPSPSLAENHLSRLLE
                     AGGTKAIDKIPPTDLPALLRLLGSSAYLSDVLIRQSKNWSEVFLRQIKIRHKTVAEHL
                     VELEPILKASKSIDEFCAGLRRHKQRQYVRIGARDLLPSVAMEETVRELTALAEASLE
                     AAYRFCRAEVEKDFGALLLPGKQEPNRFVILGMGKLGGGELNFSSDIDVIFLYEEDEG
                     ESSGGRKGKTTPREFFTNIGKKIVHAMGQITEEGFVFRIDLRLRPMGASGPLVQSVGS
                     AMIYYESWGQCWERAALIKARPSAGDRKLGAQFLKEIEPFIYRRYLDYTTVDELRHMK
                     MRIENELLTADGKERNIKLGYGGIREIEFFTQAMQLVNGGYEPGIREPNTLRALAQLA
                     QYKFIPARDRAKLSDAYRFLRQVEHKVQIVQEAHAHSIPEGEDEERALARRLGYARTR
                     KQNERELFWQDHRAHTATVRNIFDRLFYSAQKDITEEGASEIGSIWRDLDRQEIIVRG
                     LERVGFADPHKAYENLLEVRDGEVYAPPSPKRLKVMRSLGPALITEIAKSSAPDQALL
                     NLSKFSHRLGGRTGFLTLLAENPATMRLLITLFAESQFLTDLFLNRPEIIDTLIRVDL
                     TRVKKHKQEMLPELRTALGEHSDLEAKLNALRHYKTEEFIRIGLHDLGGTIEIEAVLN
                     QLSNLAEACLESAVQLTIEELNERFGAVPKGKFAVLGMGKMGGRELDYNSDLDLVFIY
                     DAHEDAQSRGGTQGQLGAHEYYVRVGQKLITYLSAQTEEGVAYKIDMQLRPSGKSGPL
                     VCSLDAFREYHQTSSLLWERQALIKARFVAGDPALGKQIEKVTENFAYGQGLTPEGIG
                     EIHHLRMRMERELSGEDETRFNLKKGRGGLVDIEFLTQMLQLAHGYRHPELRRRETLR
                     ALTALSEHNILKKREYKLLTDGYLFLRTLDHRLRLEQDRSIDAFEREPGRLERIAHAL
                     GYNNLKPDHRKRTSKAGQKLLRDYESRREKIRRCYESYFLLKR"
     gene            6227..7006
                     /locus_tag="VFN58_07680"
     CDS             6227..7006
                     /locus_tag="VFN58_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET8917906.1"
                     /translation="MPKQLLFVVLAVLFAQSALAQQFYRWKDEKGQWHYSDFPPSGVT
                     AEKMETVDIPQSVSQPFVSSTGGQKKAEDSSDHTTQAASDVDPLRPASKRLLVFPPSD
                     PSKPLSEWIPVESFDSVRECSRAKALQIAGSVVQGTESGDFVDFRRLNSRCISLAEFK
                     PRKEANVIVAVTNVGSDPGGFSTWVLYGRVFNGGQTTAHNVVVRYRVRDARGIIYANG
                     EILPASHDILPLTFAEYRGQILGSAGGSDRWVETEANWSKD"
BASE COUNT         1723 a         1968 c         2000 g         1863 t
ORIGIN      
        1 aatgccagct tcccaagctg gacgtcacgg gttcgatccc cgtcgcccgc tccagatctt
       61 tccttcaagc ggttcacatt gttttgtgat aggagacttt cgccctagac gtattgaact
      121 acttgaacga tccgggatgg caaccttcag aaaaatgaag taagcaagaa ccagttagtt
      181 cgagagcaac aggaagagga gttcgatcat ggctgggttg aaactgacaa tggcactctc
      241 ccattacgat cggcacattc cgttctttga caaatctgtg caagtcgagg gcgtcgatct
      301 ccaaattctc gaagtgggcc agtctgagcc actaaagcac ggccgagatc gacacgagcg
      361 aatgttgcag aagcgtgaat ttgatgtctg tgagctttct ttgtcgtctt atctgatggc
      421 caagagtcga ggcatgccgt tcactgcgat tccagtcttt ccgcgccggc tcttcagcct
      481 atcgcagatg tggatcaatg ttgattccgg gatcaattcg ccgcgccatc taatcggtaa
      541 gcgcgtcggc ctaagcacgt ttcaaaccac cctctctgtg ctcgctaaag gagatctaca
      601 gtcggaatac gacgtaccat ggcgcaaggt ggaatggatt gtctcgaagg aagaggccgt
      661 gccgttcaag ccccaagaag gtgtccatat cgaactcgtc gctcccggaa agaaaatggg
      721 ggcgatgttg gagcgaggtg aaatttccgc gttgatggtg cctcatccgc ccgagcaagc
      781 gctaaaggga tcaccgacaa ttcggcgttt gtttgcagat cccaagcaag aagaactaaa
      841 atattttcgc aagaacggct attatcccat catgcatgtg gtcgccttca aagatgaagt
      901 tttagcaaaa aatctttggc tagcgcagag cgttatggcg gcgtttgcca aagccaagga
      961 ggcttgcatg gcgtactacg acgatcccaa ttggtcgcgc tctgtttggg gccggcatct
     1021 ctttgaagaa gaaagaatcg cgttcggcga cgatccctgg ccccacggcg taaagaaaaa
     1081 ccgggccaac ctggagcgtt tcatcggcta ttctcttgac caaggcctca tggagagcaa
     1141 gctcgaggtg gcagaccttt tcgctgccac caccttggat agctaaagag gagtgaatgt
     1201 ctcttcgggt caagtgccca acctgccacc gtgaaactga gtgggaaaat aatccccatc
     1261 gaccgttctg ctctgatcgc tgccagctca ttgatctggg agggtggact gaggagagat
     1321 atcgtatccc cggcagcgaa acccaacttc ccctggatga cgaagatgat gatggcgatg
     1381 agaaagtccg ataaactagg actcgcgtaa aagaggaagt cgccacgaca aattcatgag
     1441 cattcatccg acggccgtta tcgatcctaa ggcggaaatc gactccgaag tcgagatcgg
     1501 tccttacgtc gtcatcgaag gtccggtcag gatcaagcgt ggcacgcgag tcatggcgca
     1561 tgcgtatctg gccggctgga cggagatcgg cgagagcaac gaaatccatc cgggcgttgt
     1621 tttgggtgag gcaccccaag acaaagccta caagggggaa gaaacgtacc tccgaatagg
     1681 cgctcacaat gtctttcgcg agtatgttca ggttcaccgc ggcagcgccc cgggttcatc
     1741 gacaacgatc ggcaatgaga atttcctcat ggcatgttcg catatcggtc acaacagcaa
     1801 attgggtgat caggttattc ttgccaataa tgctctcctg ggtggttatg ttgaggtagg
     1861 aaacagcgtc tttatttctg gcaattgcgt cgtgcaccag ttcgtgcgga tcggcgatta
     1921 tgcgctcatg cgcggattga gcggaaccag ccgcgatgtt ccaccttacg ccattattga
     1981 ttggcagcac acggttcgtg gcgtcaatgt cgttgggcta aaacgggccg gcttcgatga
     2041 tcagcggatt cgagagatca gagaagcctt tcgcattctc ttccgaaaag gacgtaatct
     2101 ctccctggcg ataaaggaaa taaacagcca cggaaacatc aacggagatg tcttggccct
     2161 tttggatttt atcaggtcgt cgaaacgcgg agtctgtggc ggggctagcg agtaacgctt
     2221 gcgggtgcgc tgcgctgtga ctcagattac cgctttagca agaaatagct ctcgtagcat
     2281 ctgcgaattt tctctcgtct tgactcgtaa tcgcgcaaca gtttttgccc cgcctttgag
     2341 gtccttttgc ggtggtccgg tttcaagttg ttgtatccga gagcatgggc aatacgttct
     2401 aggcggccgg gctcccgttc gaacgcatcg atggaccgat cctgttccag gcgcagccga
     2461 tggtctaggg tgcgcaaaaa aagatagcca tcggtgagta gtttatattc gcgctttttc
     2521 agaatgttgt gctcacttaa tgctgtcaac gcgcgcagcg tctctctacg tctcaactcg
     2581 ggatgccgat aaccatgcgc gagttggagc atttgagtta ggaactcgat atcgactaat
     2641 ccgcctcgtc cttttttaag attgaatcgg gtttcgtctt cgccggagag ttcgcgttcc
     2701 atgcgcatgc gcagatgatg aatctcgccg atgccttccg gcgtcaatcc ttgcccgtaa
     2761 gcaaagttct ctgtgacctt ttcaatctgc ttcccgagcg ccggatctcc tgcgacaaaa
     2821 cgagctttga tcagcgcttg tcgttcccac agcagggaac tggtctggtg atactcgcgg
     2881 aaggcatcga gtgaacaaac caaggggccg gatttgcccg aggggcggag ctgcatgtcg
     2941 atcttatagg ctacaccttc ttccgtctgc gccgagagat aagtaatgag cttttgcccg
     3001 acccgaacgt agtactcgtg agcgcccagt tgaccttggg tcccgccgcg gctttgggcg
     3061 tcctcgtgcg cgtcatagat gaaaacgagg tcgaggtcgg agttataatc gagctctctg
     3121 cctcccatct tccccatacc caggacggcg aatttccctt taggaactgc accgaatctt
     3181 tcgttcagct cctcgatggt cagctggacg gcgctctcaa ggcaggcttc ggcaagattc
     3241 gagagctggt tgagcaccgc ttcgatttca atcgttcctc ccagatcgtg cagcccgata
     3301 cgaatgaact cctcggtctt atagtgccgc aaggcgttga gcttggcttc gagatcgctg
     3361 tgctccccta gcgccgttcg caactcgggg agcatttctt gtttatgttt cttcacccgc
     3421 gtgagatcaa cccggatcaa cgtgtcgatg atctcaggtc gattcaaaaa caggtcggtt
     3481 agaaactggc tctcggcaaa tagagtaatg agcagacgca tggttgccgg attctccgcc
     3541 aaaagagtga gaaatccggt ccgccctccg agccggtggc tgaactttga cagattgagc
     3601 aaggcttgat cgggtgcgct ggattttgcg atttcggtaa tcagagccgg accgaggctg
     3661 cgcattactt tgagccgttt tgggctggga ggggcataga cctcgccatc ccgcacctcg
     3721 agaaggttct cataggcttt gtgcggatcc gcaaagccga ctctctcaag ccctcgaaca
     3781 atgatctctt gccggtcaag atcgcgccag atcgacccga tctctgacgc tccttcctcg
     3841 gtgatatctt tttgtgcgct gtaaaagagc cggtcaaaaa tgtttcgtac ggtagcggta
     3901 tgagcgcgat ggtcttgcca aaaaagctcc cgctcatttt gcttcctggt gcgtgcgtaa
     3961 ccgagacggc gggccagcgc gcgttcctca tcctcgcctt cgggaatcga gtgcgcgtgc
     4021 gcctcctgga cgatctgaac cttgtgctct acctgccgga gaaagcgata cgcgtcgctg
     4081 agctttgcgc ggtcccgcgc cggaataaac ttgtactgcg caagctgcgc caacgctcgc
     4141 aacgtattgg gctcgcgtat gcccggttca tagcccccat tgaccaactg catagcctgg
     4201 gtgaaaaatt ctatttcgcg aatgccgccg tagccaagct tgatgtttct ttccttgcca
     4261 tcggcggtca agagctcatt ttcgatgcgc atcttcatat gccgcagctc atccaccgtc
     4321 gtgtagtcaa gatatcggcg gtagatgaac ggctcaattt ccttcaaaaa ctgagcccca
     4381 agtttccgat cgccagcgct tggtctagcc ttgattaagg ctgcgcgttc ccagcattga
     4441 ccccaggatt cgtagtaaat catggcggag cccacggatt gaaccagcgg tccactcgct
     4501 cccatgggac gaagcctgag gtcgatacgg aagacaaacc cctcttcagt gatttgtccc
     4561 atggcatgaa cgattttttt cccgatgttt gtgaaaaatt ctctcggtgt cgttttccct
     4621 ttccggccac ccgaactctc gccttcatct tcctcgtaaa gaaagatcac gtcgatatcg
     4681 gagctaaaat tcagctctcc gccacctagt tttcccattc caagaattac aaaccggttc
     4741 ggctcttgct ttcccggcaa tagcagagcg ccgaagtctt tctcaacttc ggctctacag
     4801 aatcggtacg cagcttccag cgacgcctcg gccagagccg ttagctctct cacggtttct
     4861 tccatggcga ccgaagggag cagatctctt gcgccgatgc ggacatattg ccgctgcttg
     4921 tgccgcctca aaccggcgca gaattcgtcg atcgatttgg acgccttaag aatcggttcc
     4981 agctcgacca gatgttcggc aacagtttta tgtcggatct taatctggcg gagaaacact
     5041 tcggaccagt ttttactctg gcgaatcaga acatcgctga gataagcgct gctccccaaa
     5101 agtcggagca gggccggtaa atctgttggc ggaatcttgt cgatggcttt ggtgccccca
     5161 gcctccaaga gtctcgagag atgattttca gccagcgaag gtgacgggct ttcctttagc
     5221 aagcgctcaa gcttttttct ttgcactgag gaggagagaa ttttgctcaa ttgactcaca
     5281 agccaatgct ccatacacga gaaagaagct ccgatattcc cgggtaacta atcattgcga
     5341 tcggtaacta acaagttacc gttttgttgt tcctctcgcc actatcaaca ccctatgcag
     5401 aggtaagtgt tcagtaattt gccaccttac atagccgagg agccattctt ttagattggc
     5461 gcgacggttc ctgcccgacg gacacggtga tcaagtgaac ctttcgaccg tctactttgg
     5521 ctacaccagt ggtaactaat cgatacaccg tagtagcaaa aaggcgccag tcatcttcgt
     5581 ttctcgtagc ttttagatct acaaggcaac aagtttcctt aattcgagag atttataccg
     5641 atcagcgcct cgtttggtat ggcggcatgt gctttgctgt gctatttttc acgttggtac
     5701 gtctaacttt gagggagggc agtccgatga aaaatctctt cttggcagtt actctgcttg
     5761 ctttgcttct gttgggcggc ggtgacgtac gagcgcaggc gtttagcccc tactactatg
     5821 atccatattg ggatgctcag tatcagcaat atcagcagta tttgcagtgg cagcagtatt
     5881 tggcgtatct gcaacaatat gatccgtact acgagctcca tgtgacccac taccagctat
     5941 acctcccacc atacccgtcg taccaaccct gctgctatac agggggcgtt attgtcccag
     6001 attggtcaac gccgatcagt ccaccgtcac gaccgattat gaatccccga ttgcgagcgc
     6061 ttgctggccc tcggtcgcaa gcggctgtca gtccactgcc aggggcggct ggtccactac
     6121 cacgggcggc cggtccactc ccaggagcgg tgagtacact gccacaagcg actggccgaa
     6181 ggtaaagacg ccggggggtt ctgaaaaacc ctggttgcag gctgttatgc caaaacagct
     6241 cctttttgtt gtgctggcag tcttgttcgc ccagagtgct ttggcccaac agttttacag
     6301 gtggaaggac gaaaaaggcc agtggcatta ttcggatttc ccgccatccg gagtcacggc
     6361 ggagaaaatg gagaccgttg acattcctca atcggtttcg caaccgttcg tttcctcaac
     6421 tggcgggcaa aagaaagcgg aagatagctc ggatcatacc acccaagctg cgtccgatgt
     6481 tgacccattg aggccagcta gcaaacgatt gctggtgttt cctccctccg acccaagcaa
     6541 acctctctct gagtggatac cagtggaatc cttcgatagc gtccgagaat gcagtagggc
     6601 caaagccttg cagattgccg gttcagtagt gcaaggcacg gagagtggtg attttgttga
     6661 ttttcgaagg ctcaatagtc gctgcatttc gttagcggaa tttaaacccc gtaaagaggc
     6721 caatgttatt gtagccgtca caaacgtagg ttccgacccc ggcggattca gcacttgggt
     6781 cctttacggt agggtattta acggtgggca gactacagcg cataacgttg tggtgagata
     6841 ccgagttcgg gacgccagag ggattatcta tgcgaatgga gagattctac cagcttcgca
     6901 cgatatcctg ccactaacgt ttgcagagta ccgtgggcag attctcggct cggcaggcgg
     6961 gagcgatcgc tgggtcgaaa cggaagctaa ttggtcaaag gattgagtaa tcacatttct
     7021 caacactggt tcctagaatg aatttgaatt tcttcgtccg agtaagtgat acgtaatttt
     7081 cggatcttgg cttaaagtag aaactgtttt gaccgccctt ggcaatgtga tatttatgcg
     7141 cttgtaacaa gtcaccccca ccattgagaa ggcttccaat gtgacgtgag gccaaaccgg
     7201 aggacgcaag ccgttcctcg gagggggaaa tgaatagaag agtttgaagc cagccgctcc
     7261 ggcgatttgt cgccaggtgt gctggctttt ttgttgtttg gattcggaaa acagaactga
     7321 acacggcaaa gcacattgtt ttagtctggt aaggtcaaac aggaatgcaa aaaggtgtga
     7381 gcaatcttcg tatgagcatc gaggtcaaaa agaaatcggc accaacggtg catccttttg
     7441 gttacgcaca gtgtctacaa agttacccgc tctctttctc cggcacgacc gcggtgcgtt
     7501 tgtaaaagag gaattctcct gtaattcaat gtattgggtt agtctgccag aaat
//
LOCUS       DASSWZ010000319         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1243862, whole genome shotgun sequence.
ACCESSION   DASSWZ010000319 DASSWZ010000000
VERSION     DASSWZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243862"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1621)
                     /locus_tag="VFN58_07685"
     CDS             complement(188..1621)
                     /locus_tag="VFN58_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009784145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET8917907.1"
                     /translation="MAEAILCQVAVIGSGPGGAVTAMTLASKGFDVVMLEEGPYLKLD
                     SCPPFSIEEMRQKYRWGGLNPAMGAPKVPLVEACCVGGGSEINSGLYHRTPPEILELW
                     RDRFAVHYLTELDLLPHFEACESALSVQLNPGPQPAASIKMKTGAERLGWKTMEVPRW
                     FKYSQAHGPNSLAPGIRQSMTETFVPAAQTAGCRIVPGVRADKLERAGAQWRVQAVRQ
                     GEPFTIGAEAVFVCCGAIETPALLRRSRIKKNIGNSLAFHPTVKVVAVFEDEINGEPA
                     DIGAQQVKEFSPRVSMGCSISSLPYLALAMTDHPDAKVNLRRQWRRMSIYYAMISGPT
                     TGIVRNVPLSPAPLIRYSLSAEDLRDLSTALVNLCRLLFAAGATHLYPGIAELPVLAS
                     ADDLTKIPLELPRDRTSLMTAHLFSSCPMGQAADFTPVDSFGRVHGYRNLFVNDASLL
                     CTAPGVNPQGTIMAHARRNALHFSKDL"
     gene            complement(1614..1994)
                     /locus_tag="VFN58_07690"
     CDS             complement(1614..1994)
                     /locus_tag="VFN58_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917908.1"
                     /translation="MLSYLSKTISTLCRDIAAMHDCSDPVCAAASYDDVTEFVVEQLR
                     KMPVFLSRPILLATAVFGVSRLFLEGSLFHNRPQDQRCFQLESWRRSRFGPSRDLIKF
                     YTSLVVLALYSRPGTAAKEIEGDG"
     gene            complement(1999..3486)
                     /locus_tag="VFN58_07695"
     CDS             complement(1999..3486)
                     /locus_tag="VFN58_07695"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET8917909.1"
                     /translation="MTKYRNVEGSDKPGHSGSESVPLVVDLDGTLLRTDLLLESALRL
                     IKQRPWLVLLMPLWLLKGRAYLKRKIFQRVQIDVSLLPPHEELLTWLRDEKARGRRLI
                     LATASDYQQACLVAEPLGLFDTVLGSDGQRNLKGREKLKTIVNVCGEEFDYVGNSSSD
                     LAIWRDCRHAILVNTSRRVERSARRAGNVLRVFPPSLGFQDALRSMRFYQWVKNLLLF
                     VPAITSHTIFNAPVAGNATLAFFSFGFAASAAYILNDLLDLEEDRRHATKKQRPFASG
                     RTLIGSGIVLAIASLSASAAIASLVPGAFVTALLTYLILTSLYSLFLKRVLVIDVLVL
                     ALLYTLRVIAGHLATGVPFSFWLLSFAFFLFLSLAFSKRAADLIQHRQDNQKAVPGRG
                     YVTTDLDAIYIAGICSGFLSSLVLALYINSDSVQLLYRRPALLWGLQPILLYYITRLW
                     ILCRRGELTDDPLLYTVRAPSTYGAALLAIMVLLAATFDVSVFWP"
BASE COUNT          965 a         1087 c         1121 g          823 t
ORIGIN      
        1 gaaataaccc gctccgccgg taaccagggc tctcagtgtt tccgctcagc tgatggtgcg
       61 aagaagggac ggcaaatgtg caaaagatcc aagtcaaagc ggccataggt aacagcccaa
      121 ttgacgcccc tatcgattcg atcaaagtct gtggtgacga gaaggatttc tgccttgcat
      181 tccatgtcta cagatccttg ctgaagtgca gggcgttgcg gcgggcatgt gccatgatgg
      241 ttccttgagg attgactccc ggcgccgtac agagaagact ggcatcgtta acaaacagat
      301 tcctgtagcc gtggacccga ccgaatgaat ccacgggagt aaaatccgca gcctgtccca
      361 ttggacagga cgaaaagaga tgagcggtca tcaggctggt tcggtctctc ggaagctcca
      421 aaggtatttt tgtcaggtcg tctgcgcttg ccaagaccgg tagctcggcg atcccgggat
      481 acagatgcgt cgctccggcg gcaaaaagca atcggcataa gttgactaag gcagtggaga
      541 gatctcgcaa atcttccgca ctcaaagaat atcggatgag cggcgccggt gaaagcggga
      601 cattgcgcac gatgccggta gtggggccgg aaatcattgc gtaatagatg ctcattcgtc
      661 gccactgacg ccgcaggttt actttggcat ccggatggtc agtcatcgca agcgcgaggt
      721 agggtaggga cgatatcgag caccccatgc tgacgcgcgg ggagaattcc ttgacttgct
      781 gcgcgccgat gtcggcaggt tcgccgttga tttcgtcttc gaagaccgcc acaactttga
      841 ccgtgggatg aaacgcgagt gaattgccaa tgtttttctt gatcctgctg cggcgcagca
      901 gagccggcgt ttctatggcg ccacaacaaa caaagaccgc ttctgcccct atcgtgaacg
      961 gttcaccctg ccgcacggct tgcacccgcc attgagcgcc ggcgcgctca agcttgtccg
     1021 ctcgtacccc cggaactatc cggcaacctg cagtctgggc cgccggcaca aaagtttcgg
     1081 tcatggattg gcgtatgcct ggggcgagac tgttcgggcc atgcgcttga gagtatttga
     1141 accagcgcgg cacctccatg gttttccagc cgagacgttc ggcgccggtt ttcattttga
     1201 tcgaagcggc cggctgaggc cccggattca actgtacgct caaggctgac tcgcacgcct
     1261 cgaaatgcgg gagtagatcc aactcagtca aataatgcac ggcgaatcga tcacgccaaa
     1321 gttccaggat ttccggcggc gtacggtggt acaaaccact attgatctcg ctgccgccac
     1381 cgacgcaaca ggcctccaca agaggcacct ttggcgcacc catggctggg ttgagcccgc
     1441 cccaacggta tttctggcgc atttcttcga tgctgaaggg cgggcatgag tcaagcttca
     1501 agtacggacc ttcttcaagc atgacgacat cgaagccttt tgacgccaag gtcatagccg
     1561 taaccgcccc acccgggccc gaaccaatga cagctacctg gcacaggatg gcctcagcca
     1621 tcgccttcta tctccttcgc cgcggttcca gggcgcgaat acaaagccag cacgacaagg
     1681 ctggtataaa acttgatcag gtcccggctt ggcccgaatc tcgagcggcg ccagctctcc
     1741 agctggaagc atcgctgatc ttgtggccgg ttgtggaaca gggaaccttc gaggaacaga
     1801 cggctcaccc cgaagacagc cgtggcgagc agaataggtc ggctgagaaa aaccggcatc
     1861 tttcgcagtt gttccacgac gaactcggtg acgtcgtcgt aggatgcggc cgcacagact
     1921 ggatcggagc aatcatgcat ggcggcaatg tcacggcaga gtgtggagat ggtcttcgac
     1981 aggtaggaca gcattgcttc acggccagaa aacactgaca tcaaaggtag ccgccagcag
     2041 caccataatg gccagcaagg ccgctccata ggtggagggg gcccttacgg tatacaggag
     2101 aggatcatcc gttagttcac ctctcctgca gagtatccaa agacgggtaa tatagtacag
     2161 caaaatcggc tgtaaccccc aaagtagtgc cggacgccga tagagcaatt gcacgctgtc
     2221 gctattgatg tagagcgcga gaaccaggga cgacagaaat ccgctgcata tgcctgcaat
     2281 atagatggca tcgagatccg ttgtcacgta cccgcgtccc ggcaccgcct tttggttgtc
     2341 ttgccgatgc tgaattaagt ctgccgcgcg cttgctgaac gcaaggctga gaaacagaaa
     2401 aaaggcgaag gataataacc agaaagaaaa aggaacccct gtggccaggt ggccggcgat
     2461 cacgcgtagc gtatacagca aagccaatac caaaacgtcg atcaccagga cacgcttcaa
     2521 aaacagcgag taaagggaag tcaatataag ataggtgagc agcgcggtca cgaaagcccc
     2581 ggggaccaag ctggctatcg ctgcgctcgc cgagagagat gcgatcgcga gtacgatgcc
     2641 ggagccgatc aacgtccggc ccgaagcgaa gggacgctgc ttcttggtgg cgtgccggcg
     2701 gtcttcctcc aagtccagta gatcgttcag aatatatgcc gcggaagcag caaagccgaa
     2761 tgagaaaaac gccaaggtgg cgttgccagc aacgggtgca ttgaagatgg tgtgcgacgt
     2821 gatcgccggg acaaagagca agagattttt tacccattgg taaaaacgca tggagcggag
     2881 ggcgtcttga aaaccaagag aaggtggaaa cacgcgcagg acatttccgg cacggcgagc
     2941 cgaacgttcc actcgcctgg aagtattcac cagtatcgca tgccggcagt cccgccagat
     3001 cgccaggtcc gaacttgaat tgccgacgta atcgaattcc tctccgcaca cattcacaat
     3061 ggtcttgagc ttctcccgcc ctttcagatt ccgctgcccg tcgcttccta gcacggtatc
     3121 gaaaagaccg agcggttccg ctaccaagca ggcttgttga taatcggatg ccgtcgcgag
     3181 aatcagacgg cggccgcgtg ccttctcgtc tctcagccag gttaacagtt cctcatgcgg
     3241 gggtaagagc gaaacgtcaa tctgaacccg ttggaaaatc ttgcgcttca gataggctcg
     3301 ccccttcagt agccaaagag gcataagtaa aacgagccaa ggcctctgct tgatcagacg
     3361 taaagcggac tccagcagga gatcggtgcg gagcaaggtt ccatccagat ccaccaccaa
     3421 aggcacgctc tccgagccgg agtggccggg tttatcgctg ccttccacat tccgatactt
     3481 ggtcattttg gcctaggtct cgagctgttt ttcctgggag tcgggtatga tcatgatatt
     3541 gacttccgac cgccccgtct attcttcttt acggttcgac agcttgcaag atgtacaaat
     3601 agaaggaaaa agccacagtt aagccatgtt ggtagggcat tgtttcctcc attgaatgta
     3661 gatttgaagc tggtgtctgg ccgaatcaga ggctttgctc acagtatcta gcgaagccca
     3721 aagcttgtgt caaatctgcc gtattcccgg tcctggcact agatgttgtg tcgtttcgcc
     3781 tcaggttacg gcccgcagcc tgagcaggaa cggctgaaaa tgtcacgaaa tttccatatt
     3841 ttttccttga caagcaaaac cgcttgtact aatttggcga aaatttctca atttttctgg
     3901 gagggagcga tggagaaaaa caaataccta gccatcagcg cgctcggctt tttcctgacg
     3961 ggctgtgcgg ggatgaccaa gaatcaagct gcactg
//
LOCUS       DASSWZ010000320         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_946693, whole genome shotgun sequence.
ACCESSION   DASSWZ010000320 DASSWZ010000000
VERSION     DASSWZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946693"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFN58_07700"
     CDS             complement(<1..383)
                     /locus_tag="VFN58_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8917910.1"
                     /translation="MEPIKTEIVVLGAGPGGYTAAFYAADRGKKVTLVEQNPRLGGIC
                     LNSGCIPSKALLHATEILREAKDSAARGIEFGEPKIDLDKLRAWKESILERLGQGIKG
                     LAQKRGVQVIHGRGHFEDSHTLRVET"
     gene            complement(391..1710)
                     /locus_tag="VFN58_07705"
     CDS             complement(391..1710)
                     /locus_tag="VFN58_07705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET8917911.1"
                     /translation="MDVKLPRLAEGVDSGTVVSILVSEGQEIKKDQPFIELETQKAVG
                     SIPSPESGTVSKIHVKEGMEVAVGQLLISISADKDSAIAKPATAEQASAQTAVERQAQ
                     THSPTSPTSTEAKSAPQAEEYRYESKSGAAPPASPSIRKIAGDLGIDLTRVKGSEAGG
                     RIVLSDVRAYIQRLQQRSFAEAKEQPGTVSQPATPQSEPIDFGKWGPIRRERMSPLRR
                     TVSRRMIESWTTIPKINQFDDADITNLLAVRKKYAATFEKKGSRLTLTAFLLKVIADG
                     LKKHPRANASIDEANNEIIYKDYYHIGVAVDTDGGLIVPVLRDVDKKNLFELSRELHE
                     LTEKTRQRKVSLEELQGGTFTISNQGSIGSGHFTPIIYAPQVAILGVGQGQPKPMVVE
                     EKIAIRTMLPLCLAYDHRVLDGADAVRFLKEIIAGLETFPEAQVKLK"
     gene            complement(1741..>3510)
                     /gene="aceE"
                     /locus_tag="VFN58_07710"
     CDS             complement(1741..>3510)
                     /gene="aceE"
                     /locus_tag="VFN58_07710"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET8917912.1"
                     /translation="DPLLAADENGLLVKRMEEAVDGDYQKYSVEPGSYTRKHFFGKYP
                     ALLEMVNHLTDDQIRKLLRGGHDPKKVYAAYKAAVDHGGSPTVILAKTVKGYGLGEAG
                     EGRNVTHQQKKLNERELREFRTRFDIPLADEEVVETPFYRPPLDCPETKYVLERRNQL
                     GGFIPERKVKASALSLPDVTHYTEFFKGSGQHAMSTTMAMVRLLTKLLRHEDIGKRIV
                     PIIPDEARTFGMDALFRQIGIYSPKGQLYEPVDRETLLYYNEAKDGQILEEGITEAGA
                     MSSFIAAGTAYANLGMQMIPFYFYYSMFGFQRIGDLMWAAGDIKAKGFLLGATAGRTT
                     LNGEGLQHQDGHSHILASSIPTLLTYDPGFAYEVAVIVRDGLRRLYGEGEEYFYYLTL
                     YNENYEMPEMPNGAEEGILKGLYKFKAADKGKTHKAHILGSGPLLREALRAQQILADQ
                     FDVSADIWSATSYKLLRGDALNIKRWNMLHPLAPQKKSYLESLLQNEEGVFVAVSDYM
                     KMVPDQIAPWVPGGLTTLGTDGFGRSDTRPNLRRFFEVDAETTVIATLYALYQKGSLP
                     AQAVDAAIKKLDVNPDKSFPFYL"
BASE COUNT          699 a          962 c          970 g          879 t
ORIGIN      
        1 gtttcgacgc gaagagtgtg cgaatcttcg aagtgacctc taccgtgaat cacttggacg
       61 ccgcgttttt gggccagacc tttgattcct tgacccagcc tctccaggat cgattccttc
      121 caagcgcgga gcttatcgag atcgatttta ggctcaccga attcgatacc ccgcgcggcg
      181 gaatccttgg cttcgcgaag aatttccgtg gcgtgcagca atgccttgga gggaatgcat
      241 ccgctgttga gacaaattcc cccgagccgc ggattttgct ccaccagggt tacctttttg
      301 cccctgtcgg cggcataaaa ggcggcggta tagcctccgg gcccggcccc aaggacgaca
      361 atctcggttt taatgggttc cattttttct ttacttgagc ttcacttgag cttcgggaaa
      421 ggtttccaag ccggcgatga tctcttttag aaagcgcacg gcatcggcgc cgtcaagcac
      481 ccggtgatcg tacgccagac agagggggag catggtgcga atcgcgatct tttcttcgac
      541 caccatgggc tttggttggc cttggccgac gcctagaatc gccacctgag gggcgtagat
      601 aatcggagtg aaatgtccgc tgccgatgct tccctgattg gagatcgtga acgtgccgcc
      661 ttgaagctct tccagagata ctttccgctg ccgggtcttt tcggttagtt cgtgcagctc
      721 tcgggaaagc tcgaaaaggt tttttttgtc cacgtcgcgc agaaccggca cgattagccc
      781 gccatcggtg tcgacggcga caccgatgtg gtaataatct ttatagataa tctcgttgtt
      841 ggcttcgtca atactggcat tggcccgagg atgctttttt aggccatccg caatgacttt
      901 gagaagaaaa gcggtcaacg tcaaccggga tccctttttt tcaaaggtcg cggcgtattt
      961 cttacgcacc gccaggagat tcgtaatatc cgcatcgtca aattgattga ttttaggaat
     1021 ggtcgtccaa gactcgatca tccggcggct aacggttcgc ctcagaggtg acatccgctc
     1081 ccgtcgaata gggccccatt tgccgaaatc gatgggctca ctttgtggcg ttgccggttg
     1141 tgaaacggta ccgggctgct ctttagcttc agcaaacgac cgctgctgca gccgctggat
     1201 gtaagcgcgc acgtccgaga gcacaatcct gcctccggct tcactgccct taactcgcgt
     1261 caggtcgatg ccgagatcac cggcgatttt gcgaatcgac ggggaggcgg gcggtgctgc
     1321 gccgcttttg gactcgtacc ggtattcctc cgcttgtggg gcggatttcg cttccgtgct
     1381 tgtcggcgac gttggactgt gagtttgtgc ctggcgctct accgctgttt gcgcactcgc
     1441 ttgctccgcc gttgccggct tggcgatggc ggagtctttg tcggcggaga tcgagataag
     1501 gagttggccg acggcgacct ccattccttc tttgacgtga atcttgctaa cggtgcctga
     1561 ctccggggat ggaatggagc ccacggcctt ctgggtttcc aattctatga agggctggtc
     1621 ttttttgatc tcctggcctt cggagaccag aatactgacg acggtccctg aatctacgcc
     1681 ctcagctagt cgcggaagtt tgacgtccat gaaaaaaaat cgggcgcgga aaagagttgg
     1741 ttagagataa aaggggaaag atttatccgg gttgacgtcc agttttttaa ttgccgcatc
     1801 gaccgcctgc gcgggcagcg agcctttttg atagagcgca tagagggtgg cgatgactgt
     1861 ggtttccgca tccacttcga aaaagcgccg gagattcggg cgggtgtcac tccggccgaa
     1921 cccgtccgtc ccgagcgtgg tcaggccgcc aggtacccat ggagcaattt ggtccggtac
     1981 cattttcata tagtccgaaa ccgcgacgaa tacgccctct tcattctgaa gcaaggattc
     2041 gagatatgat tttttctgag gcgccagtgg atgcagcata ttccagcgct tgatgttgag
     2101 cgcatcccca cgtaagagct tgtagctcgt cgcgctccag atatcggcgg agacgtcgaa
     2161 ttggtccgca agtatctgtt gcgccctgag ggcttctctg agaagcggcc cgctaccgag
     2221 aatgtgagcc ttatgggttt ttcctttatc agctgctttg aatttgtaca gtcctttgag
     2281 aatgccttct tctgcgccgt tgggcatttc cggcatttcg tagttttcgt tatagagagt
     2341 caggtagtag aaatactctt ccccttcacc atagagccgc cgtaacccgt cgcgaacaat
     2401 caccgcgacc tcataggcaa atcctggatc gtaggttaac aacgttggaa tgctgctggc
     2461 caggatgtgg ctgtgaccgt cctggtgctg caatccttcg ccgttcaaag tcgttcgacc
     2521 ggctgtggca cccagtaaaa agcctttggc cttgatatca ccggcggccc acatcaggtc
     2581 tccgatgcgc tgaaatccaa acatggaata ataaaaatag aaggggatca tttgcatccc
     2641 cagattcgca taggccgtcc cggcggcaat gaacgacgac atggctccag cttcagtaat
     2701 cccttcctcc aggatttggc cgtcttttgc ctcgttgtag taaaggagcg tttcccgatc
     2761 caccggctcg taaagttggc ctttcggcga atagatgccg atctgccgga acaacgcgtc
     2821 cataccgaaa gtgcgcgcct catcgggtat gatggggacg atgcgtttgc cgatgtcctc
     2881 atgacgcaga agtttcgtga gcaaacgaac catggccatg gtagtggaca tggcatgttg
     2941 acccgatcct ttgaagaatt cggtgtaatg agtcacatcg gggagactca gcgccgacgc
     3001 cttgaccttg cgctccggaa tgaatccgcc gagctggttt ctgcgttcca agacgtactt
     3061 tgtttccgga caatccaagg gcggacggta gaaaggtgtt tcgacgacct cttcatccgc
     3121 cagcggtatg tcgaagcgcg tgcgaaactc gcgcagctcg cgctcgttca gtttcttctg
     3181 ctggtgcgtg acattacgtc cctcgccggc ttcacccaac ccgtatccct tgaccgtctt
     3241 tgccaatatg acggtggggg aacccccatg atccaccgcg gccttgtacg ccgcgtacac
     3301 ttttttcgga tcatggccgc cccgcagcaa cttgcggatc tggtcatcag tcaaatgatt
     3361 gaccatctcg aggagcgccg ggtacttgcc aaaaaagtgc ttgcgggtgt aactgccggg
     3421 ctcaaccgaa tatttctggt agtccccgtc taccgcctct tccatccgtt tgaccagcag
     3481 cccgttttcg tctgcagcca gaagcggatc
//
LOCUS       DASSWZ010000321        13075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_720461, whole genome shotgun sequence.
ACCESSION   DASSWZ010000321 DASSWZ010000000
VERSION     DASSWZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720461"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFN58_07715"
     CDS             complement(<1..937)
                     /locus_tag="VFN58_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917913.1"
                     /translation="MSQKLGVAVFLVTLILPVNFSSAAESKNYPPPRFPSYVRPPKSI
                     EDVMPFARAAVRQTGGRTPLGLVEQGQTVAIFTEPGAEEMVMQAIKRAYEERGVKIQI
                     VPEHQLLGVSREEALKAVKANRWFTSEQGYMEVRAWLLQRFGDAEVPKKWLKERRPDL
                     YKLVFQREDDTAQELVDLGRKFGGKSVGEAIVKYLDQNKDINAVFWRRGGRTVTRKSI
                     EPYGSKYFGNFIFDNRYELMNKAATFPGDLWRLAEERVVESLSWVDRVEVNDPEGTNF
                     AFELDEKQAQSWAEGAYQQGHLFMFPHQATGRFPYS"
     gene            complement(993..2690)
                     /locus_tag="VFN58_07720"
     CDS             complement(993..2690)
                     /locus_tag="VFN58_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917914.1"
                     /translation="MKLLAMGLLLLGMSLAEVAFGAEQGKYPPPRFPSYVKPPKSIED
                     IMPFARAAVRQTGGRTPLGLVEKGTLVGLVTEAIADDTVLQAIIRAYKERGVEARIIP
                     EHELAGVSREEVLQAIKANKWYTSELGFMEIKPWITQRFADPEVPKKWLRERRPDIYK
                     AMFSQDDEVITPAHKEIFNKLAQRNMGELLAKYLDSHPEVKGVFWRRGGRPNTRKAMK
                     HQGEKLLGNFIFDNHWELMNKAATFPGDVWKLTEERVIESFAWVDQVHVTDPEGTNFT
                     FSLTEKEAEVWAEGAYQQGHLYLYPTQATRGFPYSKVDYPALTKHWLAPVLIKVNGVF
                     AGTNNHYGTYPRIEVTVKDGIVKEVKGGGTYGDLWREFIKYPQINEAQYPFMPEKGYW
                     WLHEAGLGTNPKFFKRPDENMEGNNISERNNAGVIHWGFGLNMLHGPKEALLPKEWID
                     FTKTANLPDDHGWHIHNLLPTYRVKVRGTKNSWITLIDKGELTAFRSPEVRALASRYG
                     DPRDVLSDDWAPHLPGINAPGKYEDYAKDPWKTISGVIKRIQAGNYEGFYPPVKPKQ"
     gene            complement(2701..4395)
                     /locus_tag="VFN58_07725"
     CDS             complement(2701..4395)
                     /locus_tag="VFN58_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917915.1"
                     /translation="MKTIALMMAVICLGLASLVAGAENTKYPTPRFPSYLRPPKSIDD
                     VMPFARAAVRQTGGRTPLGLVEKGMGALIVSEVSADAMVMQAIKKAYEERGVKVYIVS
                     EDELLEINKEEAQKAIQAMHWYTSEQGYMEVRRWIDDLFVDAEVPKKWLKERRPDLYN
                     AIYTKGEEVPQKQRETAKQFGGGNVAEAIVKYLDQHKDINAVFWRRGGRPRTARLLKQ
                     HSAKFYGNFIFDNRYELMNKAATFPGDVWRLAEERVLESLAWLDRVEASDPEGTKMHF
                     ELTEEEAKIWASGAYNQGHLFMSPYQATGRFPYSVIDYPAIQKKWNAPLQTKVTGVFA
                     GTSNHTGSFPRIEVHVKDGYVTDVKGGGPYGELWREFLKYPKINELTYPYQDKPGYWW
                     FYEAGLGTNPKFFKRPDENMLGNNTSERNNSGVIHWGFGGSVVHDPDKPEESKAWIDF
                     PKQNGLPKDHWWHIHNMMVTYRGKVRGTKNSWITLIDKGELAAYKSPEIRALASRYGD
                     PSEVLAEDWVPHIPGITAPGRYEDYAKDPWKTFTEVMKKVNAGTYEFFYPVARSKK"
     gene            4593..5996
                     /locus_tag="VFN58_07730"
     CDS             4593..5996
                     /locus_tag="VFN58_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET8917916.1"
                     /translation="MSTRTQILVLGLAVGLLHGCAQFPLTLVRSAPLSLGSPPENPAD
                     GPSRQPPPTVEYGEIAAVLAQQVDVREAELLPPATLDTSSESAHEAPPEPDQQEANAK
                     TTPGSSDSPVSRPASGSRAEDRFLNSLEKDLNKAIEQPAERRRLEFSKAVIENPRVRY
                     FVNQFSKSGRNDLEKVLARSGKYMAMIVQVLHEEGVPEELAYLALIESGFVTHSSSPS
                     GAVGLWQFVPGTARRYGLKIDSWVDERRDPVKSTRAAAAYLKDLHNYFGRWYLAIAAY
                     NAGQGAIEKAIQSSGANDFWTLNAKAKLREETRNFVPKFVAASLIASDPQKYGFIDVA
                     YEPPMDYEEMEVQGSLRFASLAEMAGTDPETIRDLNPALLRGQTPPEESAFRVKLPAG
                     HATIFAQGYQERETRQAQFVTHEVKKGETLFAIARRYGQHVRSLMELNGLANPTLRVG
                     QLLKVIMIDGLRSGILR"
     gene            complement(6011..7297)
                     /locus_tag="VFN58_07735"
     CDS             complement(6011..7297)
                     /locus_tag="VFN58_07735"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET8917917.1"
                     /translation="MASETLTIIDNRTGKKYEIPIRHGAIKATDLFQIKVAEEDGLVS
                     YDPGFMNTASCQSKITYIDGDRGILRYRGYAIEQIAEQSNYLETAYLILHGELPTQSQ
                     LADWTHNITYHSIIHENIKKLIDGFHHDAHPMGILVSTVGALSTFYPGAKKIFDPQSR
                     REQTYRLISKMPTLAAFAYRHSLGLPYSYPDNDLSYTGNFLNMLFKMTELKYKPNPVL
                     ERALDVLFILHADHEQNCSTNAMRGVGSSQSDPYSSVAAAAAALYGPLHGGANEAVLR
                     MLMAIGSKDKVPEFIKKVKGGEGRLMGFGHRVYKNYDPRAKIIKEIADQVFEVKGRNP
                     LLDIALELERIALEDGYFIQRKLYPNVDFYSGLIYQSMGLPMDMFPVLFAIPRTSGWI
                     AQWEEMLTDAEQKLARPKQLYLGADQRDYVPMEKRK"
     gene            complement(7378..7836)
                     /gene="sixA"
                     /locus_tag="VFN58_07740"
     CDS             complement(7378..7836)
                     /gene="sixA"
                     /locus_tag="VFN58_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HET8917918.1"
                     /translation="MNIYLVRHGEAVSEKLDPERPLTASGREAVERIAGMAATKNVTV
                     TTIFHSGILRARQTAEILAEPLRCTSGVQPLSGLRPQDDPAIAKAEIEAAESPIMLVG
                     HLPHLNRLLALLLSGDPESQVMEFPPAAMVCCSNDMSKWKISWTLTSQPL"
     gene            8032..8760
                     /locus_tag="VFN58_07745"
     CDS             8032..8760
                     /locus_tag="VFN58_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET8917919.1"
                     /translation="MIEFHDVDKWFGSLHVLKSINLKINGGEVVVVCGPSGSGKSTLI
                     RCINRLEPIQSGEIVVDGQSLSDPKFDPTKLRTHVGMVFQSFNLYPHMTVLQNITLAP
                     TKVKGLAIREAEKIATQLLERVGIPDKVGVYPANLSGGQQQRVAIARALAMKPKIMLF
                     DEPTSALDPEMINEVLEVMTDLAHDGMTMVVVTHEMGFARRVAHRVIFMDEGQIIEES
                     KPEDFFGAPQSPRAKQFLSKILSH"
     gene            8771..9628
                     /locus_tag="VFN58_07750"
     CDS             8771..9628
                     /locus_tag="VFN58_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET8917920.1"
                     /translation="MRRLLLLLATVIAIAGSITVSRAQTTLEKINQTGVLTIGTRTGS
                     PPFAYVNKNNEWVGFSIDLVETAILPLISKKLNKQIKLEKKESAPNTRIPLLTSNAVD
                     LIAETMTDTQSRRDQVDFSLTFFATGAQFLVKRGTPIKGIQTIAGKRIAAQQGSTNAK
                     IIRERVPTAKLLEFPDQPAAFQALAQGQVQAYTNDGIQLAGLKAKAPKPDEWAVVGEF
                     FSYEPYGMAMRKNDSDFRQAVNVGLMEAIESGKYFELYEKWFGPKGEVPYPLAPETKR
                     FLQMQVVPK"
     gene            9774..10499
                     /locus_tag="VFN58_07755"
     CDS             9774..10499
                     /locus_tag="VFN58_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8917921.1"
                     /translation="MTYQFDWSVLWRGQSGLWLLQGLITTLEISAIAWLLAITLGILS
                     GALRTAAITPLRWLASSYVEFFRNVPLLVWMFFWYFAVPPLLAQPVQDWLFDHGAEFW
                     AGVVALGVYHGARFSEVIRSGIQSIPKTQFEAALSTGLTMAQAYRLIIIPIALRLIIP
                     PATNESLNLIKNSSVALTIGVAELTFQTRQIETYTAKAFEALTAGTLIYLMLCLTIAT
                     VMSQLEKRFAIPGMITKRQPGEF"
     gene            10502..11197
                     /locus_tag="VFN58_07760"
     CDS             10502..11197
                     /locus_tag="VFN58_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET8917922.1"
                     /translation="MDFGVIINNARFLILQGFLGLGNFTGGTLRLAVPAIIFGFFLGV
                     FIGMGRLSRSRWIHVAATLYVEFFRGVPLVMVIFWFWFIIPVLVGRSFPEYGVALAAF
                     VIFEAAYFGEIVRAGIQSVPRGQAEAATATGLTRAQTMRYVILPQALRNMIPALVTQS
                     IVLLKDTSLASIIGYVDLTKAAQIVNNREIRPFELYLFIAVIYWLCSYGMSRYAGRIE
                     RRLAVNPVQAPGL"
     gene            11338..12288
                     /locus_tag="VFN58_07765"
     CDS             11338..12288
                     /locus_tag="VFN58_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET8917923.1"
                     /translation="MLFLKNTPIDQIVSMDEIIVAIEDSLKEMALGRGFDLPRRRIHH
                     PNRMIFGLLPGSVHGVMGAYLQTDLDRQLHHETVILYSVETGEPLILFQDCSINEFRT
                     GAAGGIGAKYLARKNSSRVAVFGSRVHAETQLKAAAAVRTLTQVKVFSPNEVRRRAFA
                     EKMSRELAIEINVASSPEEALEGADIVITATNSRRPVFDGNWLKEGTHITSIANGDRT
                     RTRQEIDETTLRRAATIFITSKETVCVNESDIFRALRDKVTSWDRVREISSLLLGKVT
                     GRTDDDQITLYKLQGIGIMDVAVGLRAYERIKDSNVATRL"
     gene            12317..>13075
                     /locus_tag="VFN58_07770"
     CDS             12317..>13075
                     /locus_tag="VFN58_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET8917924.1"
                     /translation="MAHYDMLVIGSGPAGQKAAIQAAKVGKRVGIVERKKVVGGICIN
                     TGTIPSKSLREAVLFLSGFRQRNLYGASYRVKKDITFEDLAMRCDHVVKAEQEVIQNQ
                     LIRNHVDFIIGTATFLDPHRLSIKQESETNEHTADFIVVAVGTETARPPEIEFDSESI
                     IDSDGLLTLKQLPTSLTIVGGGVIGCEYASILATLGIPVVLVERRPRLLEFVDSELIE
                     ALQYQMRSIGVTLRFNEEVVRIEKSPDNSVAIHLK"
BASE COUNT         2883 a         3644 c         3444 g         3104 t
ORIGIN      
        1 acgaataggg aaatcggccg gtcgcttgat ggggaaacat gaaaaggtgt ccctgctggt
       61 aagcgccttc ggcccacgat tgcgcctgtt tctcgtccaa ttcgaaagca aagttcgtcc
      121 cctccgggtc attgacttcg acgcggtcga cccaggagag cgactccacg accctttctt
      181 cggccagccg ccaaagatct cctgggaacg tcgctgcttt gttcatcaat tcgtaccgat
      241 tgtcgaagat gaagttgccg aaatacttgc tcccgtacgg ctcgatggat ttgcgggtca
      301 ccgtccgccc gccgcgccgc cagaaaacgg cgttgatgtc tttattctgg tcgagatact
      361 tgacgatcgc ttcgccgacg cttttgccgc cgaattttct acctaaatcg acaagttctt
      421 gcgcggtgtc atcctcgcgc tggaaaacta gcttatagag atccggtcgc cgttctttga
      481 gccacttttt cggaacttcg gcatcgccga aacgttgcaa gagccacgcg cgaacctcca
      541 tgtacccttg ttccgaagtg aaccagcggt tcgcctttac cgccttaagg gcttcctcgc
      601 ggctcacccc caagagttgg tgttcgggca caatctggat cttcacgccg cgctcctcgt
      661 aagcgcgctt gatcgcctgc atcaccatct cctccgcccc gggctcggta aaaatcgcta
      721 ccgtttgccc ttgctcaacc aagcccaacg gagtcctacc gccggtctgt cgcacggcgg
      781 cacgggcaaa cggcatgaca tcttcaatcg atttcggcgg cctgacatag gatggaaagc
      841 gcggcggagg ataattctta ctctccgcag cgctagagaa attgactgga agaatcaacg
      901 tgactagaaa caccgcgacg ccaagctttt gactcatgtc aaacctcccg ggtttgagcc
      961 attgaacctt tgaacttcga accgcaagcg tgtcactgtt tcggcttcac aggcggatag
     1021 aaaccttcgt agtttccggc ttgaattcgc ttgatcactc ccgaaatcgt cttccacggg
     1081 tccttggcgt aatcctcgta cttgcccggc gcgttgatgc ccggcaggtg gggcgcccag
     1141 tcgtcgctga gcacgtcgcg tggatcgccg taacgcgatg ccaacgcgcg cacctcggga
     1201 cttctgaagg cggtcaactc gcctttatcg atcaatgtga tccagctatt cttggtcccc
     1261 cgcactttca ctctgtaggt cggcaacaaa ttatgaatat gccagccgtg gtcatccggc
     1321 agatttgcgg tcttggtgaa gtcaatccat tccttaggca agagcgcttc cttcggtccg
     1381 tgcagcatgt tcaagccgaa gccccagtga atcacgccgg cgttgttgcg ctctgagata
     1441 ttgttgccct ccatgttctc atcgggtcgt ttaaaaaact ttgggttggt tccgagccca
     1501 gcctcgtgca accaccagta gcccttttcg ggcatgaacg gatactgcgc ttcgttgatc
     1561 tgtggatatt tgataaattc gcgccagaga tcaccatagg taccgccgcc cttcacctcc
     1621 ttaacgatac cgtctttcac ggtcacttcg atcctgggat acgtcccata gtggttgttg
     1681 gtgccggcga acacgccgtt cactttgatg agcaccggag cgagccaatg cttggtcagg
     1741 gcgggatagt caacctttga atacgggaag ccgcgcgtcg cctgggttgg atagagataa
     1801 agatggccct gctgataagc cccttctgcc catacttccg cttctttctc agtcagtgag
     1861 aaagtgaagt tggtgccctc gggatcggtg acgtgcacct gatccaccca cgcgaacgac
     1921 tcgatgaccc gctcttcggt gagcttccac acatcgccgg ggaaagtcgc cgctttgttc
     1981 atcagctccc aatggttgtc gaatatgaag ttgccgagga gcttttcccc ctggtgcttc
     2041 atcgccttgc gtgtattggg ccggccgccg cgccgccaga aaacgccctt tacttcggga
     2101 tgcgaatcca gatatttggc tagcagttcg cccatatttc tttgcgcgag cttgttgaaa
     2161 atctctttgt gcgccggcgt aataacctca tcgtcctgtg agaacatagc cttatagata
     2221 tccggccggc gttcgcgcag ccactttttc ggcacttcgg gatcggcaaa gcgttgggtg
     2281 atccagggtt tgatctccat gaaacccaat tccgatgtgt accacttgtt cgctttgatc
     2341 gcttgcaaaa cttcctcacg gctgacgccc gcaagctcat gctccggaat gatgcgcgct
     2401 tcgacgcccc gctctttgta agcgcggatg atcgcctgca gcactgtatc gtcagcgatg
     2461 gcctcagtga ccaggccgac caatgttcct ttttcgacca aacccaacgg agtccggccg
     2521 ccggtctggc gcacggcggc gcgcgcgaaa ggcatgatgt cttcaatcga tttcggcggc
     2581 ttaacatagg acggaaagcg cggtggggga tatttccctt gctcagcacc gaaagcgacc
     2641 tcggcaagac tcatcccaag taaaagcaag cccatagcta acaatttcat gatacgcctc
     2701 ctatttcttg gatcttgcga ccgggtagaa aaactcgtag gtcccggcat ttactttttt
     2761 catgacttcg gtaaatgtct tccacggatc cttcgcgtaa tcttcatagc ggccgggcgc
     2821 ggtgataccc ggaatgtgcg gcacccaatc ttccgccagc acttcgcttg gatcgccgta
     2881 gcgcgatgcc agcgcacgaa tctcaggact tttgtatgcc gcgagctcgc ctttgtcgat
     2941 gagcgtgatc caactgttct tcgttccccg tactttcccc cggtaagtga ccatcatatt
     3001 gtgaatatgc caccagtggt ctttgggcaa accgttttgt ttgggaaagt cgatccacgc
     3061 cttcgactcc tccggcttgt ccggatcgtg gacgacgctg ccgccgaagc cccagtggat
     3121 aacaccggaa ttgtttcgct ccgaagtgtt attgccgagc atgttttcat ccggccgttt
     3181 gaagaatttt gggttggtcc ccaaacccgc ctcataaaac caccagtagc cgggtttgtc
     3241 ctgataggga taggttagct cattgatctt cggatatttg agaaactctc gccacagctc
     3301 accatagggt ccaccgcctt taacgtcagt aacgtatccg tccttcacgt gaacttcgat
     3361 ccgcggaaac gaaccggtgt ggttgctggt gccggcaaag accccggtga ctttggtctg
     3421 gagcggcgcg ttccattttt tctgaatcgc cggataatct atgacggagt acgggaaacg
     3481 acccgtcgct tgatacggcg acatgaaaag atggccctga ttgtatgccc cggaggccca
     3541 gatctttgcc tcttcttcgg tcaattcgaa atgcatcttg gttccttccg ggtcggatgc
     3601 ctccacgcga tccagccaag ccagcgactc cagcactcgc tcttcggcta aacgccagac
     3661 gtcgcccggg aacgtggccg ccttgttcat caattcgtag cgattatcga aaatgaaatt
     3721 gccgtaaaat ttggcgctgt gctgcttgag gagtcgcgcg gtgcgcggcc ggccaccacg
     3781 gcgccagaaa accgcgttga tatctttgtg ctgatccaga tactttacga tcgcctctgc
     3841 aacgttgccg cccccgaact gttttgccgt ttcccgttgt ttctgaggca cctcctcgcc
     3901 cttggtgtaa atggcattgt acaagtcggg ccgccgttcc ttcagccact tctttggcac
     3961 ttccgcatcg acgaacaaat cgtcgatcca tcgacggact tccatgtaac cttgctcgga
     4021 cgtataccaa tgcatggcct ggatcgcttt ttgtgcttct tctttgttaa tttccaagag
     4081 ctcgtcctcg gatacgatgt agactttgac gccgcgttcc tcgtaggcct tcttgatcgc
     4141 ctgcatcacc atcgcatccg cgctaacctc gcttacaatc agtgcaccca tacctttttc
     4201 gaccaagccc agcggggtgc gccccccggt ttggcgcacg gcggcccgcg caaagggcat
     4261 aacatcgtcg atggatttag ggggtcgcag ataagaggga aaccggggcg tcgggtattt
     4321 ggtattttcc gctcccgcca cgagagatgc caggcccaaa caaatcaccg ccatcatcaa
     4381 ggcgattgtc ttcacaacag cgcctccttc gtccttatac gtcgattccc tgaaatattt
     4441 tttatttttt tttgctccaa tctgtcaatc cttttcaact tcttggcatc gctgaggaat
     4501 ctagagactt agacgatcaa ttgtcaagca ctttcgggtt gcacgacgct gacgattcga
     4561 gtagccttcg cgggttcgtc ggtgaccaag caatgtcgac gcgaactcaa attctagtcc
     4621 tcggcttggc ggttggcctc ttacatggct gcgcacaatt tccactaacc ctggtgcgca
     4681 gtgcgccact ctctttaggg tcaccaccag aaaacccagc tgacggcccg tctcgtcaac
     4741 cgccacccac tgtcgagtat ggggagatag cggcggtcct tgcgcagcag gtcgatgttc
     4801 gggaggcgga gctactgccc ccggccacgc tcgacaccag ttccgaaagc gcccacgaag
     4861 caccgccaga acccgatcag caagaagcaa acgcgaaaac gacaccgggt tcgagcgatt
     4921 ctccggtctc gagaccggcc tccggatcgc gcgcagaaga tcgtttcttg aactcgttgg
     4981 agaaggatct taacaaagcc atagagcaac ccgccgagcg acgtcgatta gaattctcca
     5041 aagcggtcat cgaaaaccca agggtccgct actttgtcaa ccaattttcc aaaagcggta
     5101 gaaacgacct cgaaaaggtc ctggcccgct ccggaaagta tatggcgatg atcgtacagg
     5161 tcctgcacga ggaaggagtt ccggaagagc tagcctacct ggcgctcatc gaaagcggtt
     5221 ttgtcaccca cagctcatcc ccttcagggg ccgtcggact ttggcaattc gttcccggca
     5281 ccgcgcgtag gtacggtttg aaaattgatt cgtgggtgga cgagcgccgg gatccggtaa
     5341 aatcaacccg ggccgccgct gcctatctca aggatctgca caactacttt ggtcgatggt
     5401 atctggccat agcggcctac aatgcagggc agggtgccat tgaaaaggcc atacagagtt
     5461 ccggagcaaa cgatttttgg acgctaaacg cgaaggctaa gttaagagag gaaacacgta
     5521 acttcgttcc caagttcgtg gcagcgtcgc tgatcgcaag cgatccgcaa aaatacggtt
     5581 tcattgacgt ggcgtatgaa cccccgatgg actacgaaga aatggaagtt caggggagtt
     5641 tgcgcttcgc ttctttggct gaaatggcag gtactgatcc ggaaacgatc cgagatctaa
     5701 acccggcatt actccggggc cagaccccgc cggaggagag tgctttccgc gtgaagcttc
     5761 cggccggaca cgcgacaata ttcgcgcaag gctatcaaga acgcgaaacg agacaagctc
     5821 agttcgtcac ccacgaggtc aaaaaaggag aaacgctctt tgccatcgcc cgccgctacg
     5881 gtcaacacgt taggtctttg atggagctca acggccttgc aaatccaacc ctacgggtcg
     5941 gtcagttgct aaaagtaata atgattgacg gattacgcag cggaatacta aggtaagacc
     6001 tggctctttt ctactttctc ttttccatcg gcacatagtc gcgctggtca gcgccaagat
     6061 aaagctgctt cgggcgcgcc aatttttgtt ccgcatcggt gagcatttct tcccattgag
     6121 caatccatcc cgaggtgcgc ggaatggcga acaaaaccgg aaacatgtcc atgggtaggc
     6181 ccatggactg gtagattaga ccagaataga agtcgacgtt cggatagagc ttgcgctgaa
     6241 tgaagtaacc gtcctcgaga gcgatacgtt cgagttcgag agcgatatca agcagcgggt
     6301 tgcgtccttt gacctcaaag acctggtcgg caatttcctt gatgatcttg gcgcgcgggt
     6361 cgtaattctt gtagacccga tggccaaagc ccatcaatcg cccctcgccg cccttgacct
     6421 tcttgatgaa ctcaggaact ttgtctttcg agccgatcgc catcagcatt cgcagcacag
     6481 cttcattggc gcctccgtgg agcgggccat aaagagccgc cgccgccgcc gcgacggagg
     6541 aataagggtc ggactgggag ctgccgacac cgcgcatggc gttagtgctg caattttgct
     6601 cgtgatccgc gtggagaata aaaaggacat ccaaggctcg ctcaagcacc ggattgggct
     6661 tgtacttgag ctccgtcatt ttgaagagca tgttcagaaa gttgccagtg taactgaggt
     6721 cgttatccgg gtatgaatag ggcaagccga ggctatggcg ataggcgaag gcggccaagg
     6781 tcggcatctt actgatgaga cggtaggttt gctccctccg ggactgggga tcgaagattt
     6841 tcttggcacc tggataaaaa gtcgataagg ctccaacggt actgaccaaa attcccatgg
     6901 ggtgggcgtc atgatggaac ccgtcaatga gtttcttgat gttttcgtga atgatggagt
     6961 gataagtgat gttgtgtgtc cagtccgcca attgcgattg ggtcggcagc tcgccgtgga
     7021 gaatcagata agcggtttct aaataattgc tttgttccgc aatctgctcg atggcgtagc
     7081 cccgatagcg caggataccc ctgtcgccat caatgtaggt gatcttgctc tggcaggagg
     7141 ctgtgttcat gaagcccgga tcataactaa ccaggccgtc ctcttcggca accttgatct
     7201 gaaacaggtc tgtcgctttg atcgcgccgt ggcggatagg aatctcgtac tttttgccgg
     7261 tacggttgtc gatgattgtc aacgtctctg aagccattgc atctgccttt caattactcc
     7321 taagcgtttt ccatttcctt tttagaccat cccctgtcgc tggtcaacta tggggggcta
     7381 cagaggctgc gacgtcaacg tccaggagat tttccatttt gacatgtcgt tgctacagca
     7441 aaccatagcc gccggaggga actccataac ctgcgactca gggtccccgc taagcaacaa
     7501 ggccagcagc cggtttagat ggggaagatg gcccaccagc atgatgggac tttccgctgc
     7561 ctcgatctcg gctttggcga tggcgggatc gtcttgcggt cgtaagcccg agagtggctg
     7621 tacgccggag gtgcatcgga gaggctcagc cagtatctcg gcggtttgcc tagcgcgcag
     7681 gattcccgaa tggaatatgg tagtgaccgt gacatttttg gtcgccgcca ttccggcgat
     7741 acgctcgact gcttcccgcc ccgaggcagt gagcggtctt tccggatcca atttttccga
     7801 caccgcctcg ccgtgccgta cgagataaat gttcatgcgc tactaaatcc tcttcgattt
     7861 attcgcgagg tgaatctttg aaagaaaagt tgccggccgg acaaaatgcc gtccgtccaa
     7921 gcgagatatt tctatacagg acgcgttcgt gtctgttgca aaaagccctt gttcatatag
     7981 tatgcgaacc ggtattttgc aatcgtgatg acgcaaaatt aagctcttcc cgtgatcgaa
     8041 tttcacgatg tagacaagtg gtttggctcg ctccacgtcc tcaaaagcat aaacctgaaa
     8101 atcaacggag gcgaagtcgt ggtggtgtgt gggcccagtg gcagcggcaa gagcacgctc
     8161 atccgctgta tcaatcggct ggaaccgatt cagtcgggag agatcgtcgt agacgggcag
     8221 tctctttcgg atcccaagtt cgatccgacg aaacttcgga cccatgtggg gatggttttt
     8281 cagtccttta atctttatcc tcacatgacc gtgctacaga acattactct tgcgccgacg
     8341 aaggtaaagg gattggctat ccgggaagcc gaaaaaattg ccacacagtt gctggagcgt
     8401 gtcgggatac ccgacaaagt cggggtctac ccggcgaacc tttctggcgg gcagcagcaa
     8461 cgggtggcca ttgctcgtgc cctagcgatg aagccaaaga tcatgctttt tgatgaaccc
     8521 acttcggctt tggaccccga aatgattaac gaagttttgg aagttatgac cgacctcgcg
     8581 cacgacggca tgaccatggt cgtggtcacc catgagatgg gctttgcccg acgggtcgct
     8641 caccgggtta ttttcatgga tgaagggcag atcatcgaag aaagcaagcc ggaagatttt
     8701 tttggcgcgc cacaatcacc cagagccaag caatttttaa gcaaaattct ttcacattaa
     8761 ggagaatcca atgagacgat tgctactctt gctcgccacc gttatcgcga ttgcgggaag
     8821 catcactgtt tcccgagcgc aaaccacact ggaaaagatc aaccagaccg gggtcttgac
     8881 gatcggtacg cgtaccggct cgccaccgtt cgcctacgta aacaagaaca acgagtgggt
     8941 aggattttcg atcgaccttg tggaaacagc gatcttgccg ttgatctcga aaaagctcaa
     9001 caagcagatc aagctagaga aaaaagaatc ggcgccgaat actcgtatcc cgctcttgac
     9061 ttcgaacgcg gtggacttaa tcgccgagac catgaccgat acccagtcgc gccgggacca
     9121 agtcgatttt agtttgactt ttttcgccac tggcgcgcag tttctcgtca agagaggcac
     9181 cccgatcaaa ggaattcaaa ccatcgccgg caaacgcatt gcggcccaac agggttccac
     9241 gaatgcgaaa attatacgcg agcgcgtgcc gacagcgaag ttgttggaat ttccagatca
     9301 accggcggca ttccaagcac ttgcccaagg acaagtgcaa gcctatacga acgatggaat
     9361 tcaactcgcc ggtctgaagg ccaaagcgcc caagccggat gaatgggccg tggtcggcga
     9421 attcttttcc tacgagccgt atggcatggc catgagaaag aacgactccg actttcgtca
     9481 agcagtcaac gtcggcctta tggaagccat cgaatccgga aaatacttcg agctatatga
     9541 aaaatggttc ggcccgaagg gcgaagtgcc ttacccgttg gcgccggaga ccaaacgatt
     9601 tctacaaatg caagtcgtgc ccaaatagta ctggtgtttt atatcgcgcc tttcgcatca
     9661 cgtatgatga aatattggag tagtggagta ttggagtgtt gggttataaa tccttccatt
     9721 actccaatac tccatcactc cagtactcca actctcagtc actttcttaa tcgatgacct
     9781 accagttcga ctggagtgta ctgtggcgcg gccaatccgg cctctggctc cttcaaggtc
     9841 tgatcacgac cctcgagatt tccgctatcg cgtggctgct cgccatcacc ctcgggatat
     9901 tgtccggtgc gctccgaacc gcagccatca cgccgctgcg ctggctggcg agctcttacg
     9961 tcgaattctt cagaaacgtg ccgttactcg tttggatgtt tttctggtat ttcgccgtgc
    10021 cgcccttact ggcgcagccg gtgcaggatt ggttattcga tcatggcgct gagttctggg
    10081 ccggcgttgt cgcgctgggc gtttatcacg gcgcgcgttt ctccgaggtg atccgctccg
    10141 gaattcagtc cattccgaaa acgcagttcg aagccgcgct ctcgacggga ctcacgatgg
    10201 cgcaagccta tcgtttgatc atcatcccga tcgctctgag gctgatcatt ccccccgcta
    10261 ccaacgagtc gctgaacctg atcaaaaact cctcggttgc gctgaccatc ggcgttgctg
    10321 aattgacctt ccagacacgc cagatcgaaa cctataccgc gaaggccttc gaagctttga
    10381 ctgccggaac tttgatctac ctcatgcttt gcctaaccat cgccaccgtc atgtctcagt
    10441 tggagaaaag attcgcgatc ccagggatga tcacgaaaag gcagcctgga gaattttgaa
    10501 cgtggacttc ggggttatca tcaacaatgc tcgctttctg atcttgcagg gctttcttgg
    10561 cctgggcaat tttaccggcg gaaccttgcg cttggccgtc cctgccatca tcttcggctt
    10621 ttttttgggt gttttcattg gcatgggccg tctgtcccgt tcgcgttgga ttcatgtcgc
    10681 agcaacgcta tacgtcgagt tttttcgcgg cgtgcctttg gtgatggtga tcttttggtt
    10741 ctggtttatc atcccggttc tcgtcggaag atcttttcca gaatacggcg tggctctcgc
    10801 ggccttcgtt attttcgaag cggcctactt cggcgagatc gtcagggcgg gaatccaatc
    10861 cgttccccgt ggccaggcgg aagcggccac ggcgaccgga ctcacccggg cacaaacgat
    10921 gcgttacgtt attttgcctc aagcgctgcg caacatgatc cctgctttag tgacgcaatc
    10981 tatcgtgcta ctaaaagaca cttctctggc gtcgatcatc ggctatgtcg atttgaccaa
    11041 agccgcccag atcgtgaaca accgtgaaat tcgacccttc gagctctacc tgttcatcgc
    11101 ggtgatctac tggctatgca gttacggaat gtcccgctac gccgggcgca ttgagcggcg
    11161 gctggcggtc aacccggtac aggcacccgg cctttgagca agctcttcct gaaatcatcc
    11221 aagctacacg gcgtttccat tgacgggttt caaccgactg cgttaaactc taccttaaac
    11281 acgcatccgg ttgttgcacg gctcaggaaa tctaaacttc gttcgctttc aagtgacatg
    11341 ctcttcctca aaaacactcc catcgatcag atcgtctcca tggacgagat aatcgtggcc
    11401 atcgaagact ccctcaaaga aatggcgctc ggtcgagggt tcgatctacc gcgccggaga
    11461 atccatcacc cgaatcggat gattttcggc ctcttgcccg gttcggtcca tggcgtcatg
    11521 ggagcctatc tccagactga tttggatcgc cagttacacc acgaaacggt tattctctac
    11581 agcgtagaaa ccggcgagcc tctcatactg tttcaagatt gttctatcaa tgaatttaga
    11641 accggagccg cgggcggcat cggcgccaag tacctggccc ggaaaaactc cagccgggtt
    11701 gcggtttttg gaagtagggt ccatgcggag acgcagctca aggcggctgc cgccgtcaga
    11761 actttgacgc aagtaaaagt cttcagtccc aacgaagttc gtcgtcgcgc tttcgccgaa
    11821 aagatgagcc gcgaattggc aatcgagatc aacgtcgcat cgagccctga agaagccttg
    11881 gaaggtgccg atatcgttat tacagctact aattctcgga ggccggtttt tgacggcaac
    11941 tggctgaagg aaggaaccca catcacttcg attgcaaacg gcgacagaac tcgtacgcga
    12001 caggagatcg acgaaacaac gctccgtagg gcagctacga ttttcattac ctcgaaagag
    12061 accgtatgcg tgaacgagag tgacatcttt cgcgcgttgc gagacaaggt cacttcatgg
    12121 gaccgtgtac gcgagatatc gagtctgcta cttgggaaag tgaccgggag aacagacgac
    12181 gatcagataa cgctctataa gctgcaaggt atcgggatca tggatgttgc ggtcggtttg
    12241 agagcgtacg aacgaatcaa agatagcaac gtcgcaacgc gattgtgacg agcgccttag
    12301 ctttcgggaa caagaaatgg ctcactacga catgctggtg attggctcag gtccggccgg
    12361 ccagaaagcc gctatccagg ccgccaaggt tggcaagcga gtgggcatcg tggaacgcaa
    12421 gaaagttgtc ggcggcatct gcatcaacac ggggacgatt ccgagcaaat ccctgcgtga
    12481 ggctgtcctt ttcctgtcgg gcttccggca acgcaacctg tacggggcca gctaccgggt
    12541 caaaaaggac attaccttcg aagatctcgc catgcgctgc gatcacgtgg tcaaggccga
    12601 gcaagaagtt atccagaatc aactgattcg caaccatgtc gattttatca tcgggaccgc
    12661 cacgtttctt gacccgcacc ggctctccat caagcaagaa tctgaaacca acgaacatac
    12721 tgcggacttc atcgtggttg ccgtgggtac tgagacggcg cgccctccgg agatcgagtt
    12781 cgactcggaa tcgattattg acagcgacgg cttacttaca ctgaagcagt tgccgacgtc
    12841 tttgaccata gtgggtggcg gagtgatcgg ctgcgaatac gcctcgattc tcgccactct
    12901 cgggatccct gtcgtcttgg tagaacggcg tccgcggttg ctcgagttcg tcgatagcga
    12961 gctcattgaa gccctacaat atcaaatgcg cagcatcggc gtcaccttgc gtttcaacga
    13021 ggaagtcgtc cgcattgaaa agtcaccgga caactctgta gcgatccatc tcaaa
//
LOCUS       DASSWZ010000322         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_791204, whole genome shotgun sequence.
ACCESSION   DASSWZ010000322 DASSWZ010000000
VERSION     DASSWZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791204"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VFN58_07775"
     CDS             <1..1294
                     /locus_tag="VFN58_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HET8917925.1"
                     /translation="GPAKMAIVSSSLFGTISGSAVANVVVDGTMTIPMMKKSGYPPAV
                     AAAVEATASTGGQIMPPVMGAAAFLIAEYLQIPYTEVALRALVPAVLFYLALFVQVDL
                     LAGRQGLRGVPRDELPRLGNVMRRSAGFLVPLTVLVTFLFVIPRRAEVAGLLATAASL
                     VVGLLTPGIKLGPRQLLDMLADTGRGLLDIAVITGLAGVVIGILSLTSLDFSLTFTLL
                     NVAQSSIILLLVLTAIVNMVLGLSLPTTAVYILLAVLVGPALTQAGIVPIAAHLFIFY
                     FGMLSTITPPVCLAAFTAASLAKADPMKTGWESMRLAILAYIVPFIFALTPTLLLIGH
                     WTEVTLSIITAVIGATLLGVGLVGYLFRPVGIVRRILFLVAAIGLLVPVVNTGQYAAL
                     TWASNGIGLALAILLVLVEWLARSSRARASTAVVSADS"
     gene            complement(1327..1464)
                     /locus_tag="VFN58_07780"
     CDS             complement(1327..1464)
                     /locus_tag="VFN58_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917926.1"
                     /translation="MQTLPFKQVDVFTQKPFLGNQVAVVIEAEGLATEEIRVVSTFEF
                     E"
     gene            complement(1479..1907)
                     /locus_tag="VFN58_07785"
     CDS             complement(1479..1907)
                     /locus_tag="VFN58_07785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET8917927.1"
                     /translation="MNSIRYQYGNDLDLDQVIELYNASTLGERRPVDDRQVMSDMLRH
                     ANLVVTAWDDNSLVGIARTLTDFGYVGYLSDLAVRVSHQRKGIGIQLVQKTREKMGPR
                     SMLVLLAAPKAVEYYSKIGFTKHNSAWTIKAQDPFPIRHP"
     gene            complement(2051..3046)
                     /locus_tag="VFN58_07790"
     CDS             complement(2051..3046)
                     /locus_tag="VFN58_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8917928.1"
                     /translation="MNRVTMTLLWRLVVFSLLLACPVSVHSAEPMIVGVAGPAINMVY
                     AFVARDAGLFKKYGLDVRIVVFDAGSILAQAALSGEVKVAVSSGPATIASRTQAADTI
                     IIAACVNTLPYSLVAAKGITKWDQIKGKKIAISRFGSGTDTAIRLVLKKFGLDPAKDV
                     TIIQLGTQPSRVQALAAGAIDATIISPPLDITAKKQGYQILVNIAELKIPYPQQVIET
                     TDRFIRENPHTVKNFLKGFIEGVRYAASLKEETKKAIIKYLKTTDPEILEATYQSFVQ
                     VTDETCYPNMEGIRNAMDEVAQRVTAVKSKKPEDFVNTRFLKELEKEDFFRQVTK"
BASE COUNT          683 a          887 c          921 g          878 t
ORIGIN      
        1 cgggccggct aaaatggcga ttgtatcgtc gagcctgttc ggcaccatca gcggcagcgc
       61 ggtggccaat gtagtcgtcg atggcacgat gaccattccg atgatgaaga aatcaggata
      121 tccgccggct gtagccgctg cagtggaggc gacggcgtca accggtggcc agattatgcc
      181 acccgtcatg ggcgcggcgg cctttctgat tgctgaatat ctccagatcc cctacaccga
      241 agttgcgctg agagcccttg tgcctgccgt gcttttttat ctggcgcttt tcgttcaggt
      301 tgatttactg gcgggaaggc aaggcctgcg gggtgttcca agagacgaac taccacgcct
      361 cggtaatgta atgcggcggt cggcaggttt tctcgtgcct ctaactgtgc ttgtcacctt
      421 tctctttgtc atacctcgaa gagcagaggt agcgggttta ctggcgacgg ctgcctccct
      481 ggttgttggt ctactcaccc cgggtattaa acttggcccg cggcaactcc tcgatatgct
      541 ggctgatacg ggtcgcggcc tgctggatat tgccgtgatc accggccttg ccggagtggt
      601 gatcggcatc ctttcgctta ccagcctaga tttctctctg acgtttactc ttttgaacgt
      661 cgctcaatcg agtatcattt tattgctggt tctcacggct atcgtaaata tggttcttgg
      721 gctgagcttg cccacgactg ccgtgtacat ccttttggcc gtcctggtgg gcccggctct
      781 gacccaggca ggcattgtgc ccatcgcggc gcacctgttt attttttatt ttggcatgct
      841 gagcacgatc acgccgcccg tctgcctggc cgcattcacg gctgcgtcgc tcgcgaaagc
      901 tgatccgatg aagacgggct gggagagcat gagactggct attttggctt atattgttcc
      961 cttcattttt gctttaacgc cgactttgct gctaatcggt cactggactg aagtgacgct
     1021 ctctattatc accgccgtta tcggcgcaac tttgctgggg gttgggttgg taggttatct
     1081 tttccgtccg gtaggaatcg tgcggcggat cttgtttctc gtcgctgcca ttggcttact
     1141 ggttccagtc gttaacaccg gccaatatgc cgcgttgacc tgggccagca acggtatcgg
     1201 actcgcgttg gcaattttac ttgtgttggt agagtggttg gcgcgctcat ctcgcgcccg
     1261 cgcgtcgaca gccgtggtaa gcgctgacag ctaatgggcc gtcttgcgcg tcccggcaaa
     1321 ctcacttcac tcgaattcaa acgtgctgac gacgcgaatc tcttccgttg ccaatccttc
     1381 cgcttcgata acgaccgcca cctggtttcc caagaacggc ttctgcgtaa agacatcgac
     1441 ttgcttgaag ggaagggttt gcatacttat caccctttct aaggatggcg gatcgggaag
     1501 ggatcttggg ctttgatggt ccaggcgctg ttatgcttgg tgaagccgat tttggaataa
     1561 tactcgacgg ctttcggggc cgccagcaag acgagcatcg acctcggtcc catcttctct
     1621 ctggtctttt gaaccaattg aatgccgatg cctttccgct ggtgcgatac cctcaccgcg
     1681 aggtcggaca ggtaaccgac gtagccaaag tccgtgagcg tgcgcgctat tccaaccaac
     1741 gagttgtcat cccaggcggt gacgacgaga ttcgcgtgcc ggagcatgtc ggacataacc
     1801 tgccgatcat ccaccggtct tcgttccccg agagtggaag cgttgtaaag ctcaatgact
     1861 tgatcgagat ccaagtcgtt gccgtactga taccgaatac tgttcatttt ggctgctccc
     1921 tagtcagtaa gccgctcaag ctgggggacc tgaagcttcc aaatacgaga tgctaatccg
     1981 ggccaatgga aggccgagaa tctctgctgt tcgggttaga acccgcgctt gcgttgcgca
     2041 gcgatcttaa tcattttgtg acttgtctaa agaagtcttc cttctccagc tccttcaaaa
     2101 agcgcgtgtt gacgaaatcc tcgggctttt tgctcttgac cgcggtgacg cgttgcgcca
     2161 cttcgtccat ggcgttgcgt atgccctcca tattcggata gcaggtttca tcggtgacct
     2221 ggacaaagct ctggtaagtc gcctcgagaa tttcagggtc ggtggttttc aggtatttga
     2281 tgattgcttt cttggtttct tctttcaagc tggctgcgta gcggacaccc tcgatgaaac
     2341 ctttcagaaa gttcttcacc gtgtgcggat tctcgcggat gaaacggtcc gtcgtttcga
     2401 tcacttgttg cggataggga attttgagtt ccgcgatgtt cacgagaatc tgatagccct
     2461 gttttttggc ggttatgtct aggggcggcg atatgatcgt cgcatcgatg gcgccggccg
     2521 ccaaagcctg cacgcggctc ggctgggtgc cgagctgaat gatggtgaca tccttggctg
     2581 gatcgagtcc aaattttttg agcaccaatc ggatcgcggt gtccgttccc gaaccgaagc
     2641 ggctgatggc gattttcttt cctttgattt ggtcccactt ggttattcct ttggcagcga
     2701 ccaagctata ggggagcgta ttgacgcaag cggcaataat aattgtgtct gctgcttgag
     2761 tgcgcgaagc aatggtggct ggtcccgacg acacagccac tttaacttcg cccgagagcg
     2821 cggcctgggc caagatagaa ccggcatcaa agacgacgat gcggacatcg agaccatact
     2881 tcttaaaaag gcccgcgtcc ctcgcgacaa aggcatacac catgttgatt gcagggccgg
     2941 caacgcccac gatcataggc tcagcagagt gaacggaaac cgggcaagca agcaagagcg
     3001 aaaatacgac caagcgccac agcaaagtca tggttactct attcattcat tctccggttc
     3061 aatttacaag tgtatcgatt tggatgttga gccggaaaga atgatgcatt cctcgctcac
     3121 caccggtacc gagtcaatta tagtctaaat tctagtgggt tagctagatt gcagaaatct
     3181 aatacgatac ggcagcaaat tcttgggttt cgcttgtgtc ggcggcaaga agttcaaaaa
     3241 ctagaaacga aacccgacgg tgcgtcagcc aaccatctcg aaccgacaag taggaaccag
     3301 aaacgtagac tcatctattt gaacgctgct tgcaaaagca ggactgccat tgttaattag
     3361 gcctctgtg
//
LOCUS       DASSWZ010000323         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_295855, whole genome shotgun sequence.
ACCESSION   DASSWZ010000323 DASSWZ010000000
VERSION     DASSWZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295855"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..290)
                     /locus_tag="VFN58_07795"
     CDS             complement(12..290)
                     /locus_tag="VFN58_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HET8917929.1"
                     /translation="MPVRVRVPTPLRKFTQGVDEVSAQGANVKALVEDLERNFPGIKE
                     RICDETGKIRRFVNVYVNGDDIRFLQNLETALKEGDNISIVPAIAGGC"
     gene            complement(419..1681)
                     /locus_tag="VFN58_07800"
     CDS             complement(419..1681)
                     /locus_tag="VFN58_07800"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET8917930.1"
                     /translation="MSFVRGLQCRECGHNYPKEPLHVCETCFGPLEIVYDYDAIKKSI
                     SREKITSRDKNLWRYRELLPIDEEPQVGLYSGFTPLVRARRLGQALGVKELYIKDDSV
                     NHPTFSYKDRVVSVAISKAIEFGFDTVSCASTGNLANAVAAHAARAGLNCYVFIPDGL
                     EQGKIIGSAIYGPKTVAIKGNYDDVNRLCSEIGDKYGWAFVNVNLRPYYSEGAKTHGF
                     EVAEQLGWRLPQHIVVASAGGTILPKLAKSFKELVKVGLVEDTGCKIYSAQASGCAPI
                     INALQQGTDLISPVKPNTIASSIAIGNPADGYYVLQAVRESGGWGESVTDKEILEGIH
                     LLAQTEGIFTEPAGGTEVAVTKKLIQNGKIPREESIVISITGNGYKTLEAVAASVEKP
                     LTITATLENFDELYQQLNPSVTPKAASG"
     gene            complement(1707..>2142)
                     /locus_tag="VFN58_07805"
     CDS             complement(1707..>2142)
                     /locus_tag="VFN58_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009167884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET8917931.1"
                     /translation="FSGYDFIIDGSDNFPTKFLVNDIAVSMDIAFSHAGIVRLQGQTM
                     TVVPRQSACYRCLFREPPPPEEILNCQQSGILGAVAGTVGSIQATEAIKYLAGFEEGL
                     LTDRLLTYDAKSMTFHTVDVSRDVRCTACGGETIGSSIAPST"
BASE COUNT          483 a          560 c          565 g          534 t
ORIGIN      
        1 tcggtaggaa attagcagcc cccggcaatc gccgggacga tggaaatatt atctccctct
       61 tttagcgcgg tttccaggtt ctgcaaaaaa cggatgtcat ccccgttgac gtaaacgttg
      121 acgaagcgac ggatctttcc ggtttcatcg cagatccgct ccttgatgcc ggggaaattc
      181 ctctcgagat cttccacgag cgctttaaca ttggcgccct gcgcgctaac ctcatcgacg
      241 ccctgggtaa attttctcag cggggtcgga acacgaactc gcactggcat aaaaaactac
      301 ctccttcgaa tctatgaaaa acaatagcta agaactttct aaccaagata gactcttgcc
      361 acaatccttg caagaaatag agcgctgcgg acacggtcaa tcgatggacc ggtgaatttt
      421 aaccgctcgc ggcttttggc gtcaccgacg gattgagctg ttggtagagt tcatcgaagt
      481 tttccaaagt tgcggtgatc gtcaatggtt tttcgacact ggcggcgacg gcttccagag
      541 ttttgtagcc gttgccggtg atgctgatga cgatagactc ctctcgggga attttgccat
      601 tttggatgag cttcttagtc acggccactt cggtaccccc agcaggctcg gtaaatattc
      661 cctcggtttg ggccaagaga tggatacctt caagaatttc cttgtcggtg acgctttcgc
      721 cccatccgcc cgattcccgc acggcttgga ggacataata gccatcagcc ggatttccaa
      781 tggcgatgga gctcgcgatt gtgttcggct tcaccgggct gatcaaatca gttccttgct
      841 gtagtgcgtt gatgatcggg gcgcaaccgg aagcttgagc agaatagatc ttgcagccgg
      901 tgtcttcgac gagaccgact ttgacgagtt ctttgaaaga cttggcgagt ttaggcagga
      961 tcgtcccgcc cgcggatgcg acgacgatat gttggggcag cctccagccg agctgttccg
     1021 ccacctcaaa cccatgggtc ttggcgccct cggagtaata gggccgcaaa ttgacgttga
     1081 caaaggccca gccgtatttg tcgccgattt cactgcagag tcgattgacg tcatcgtagt
     1141 tgcctttgat ggcgacggtt tttggtccgt aaatggcgga accaatgatc tttccctgtt
     1201 ccaggccgtc ggggataaaa acatagcagt tcaacccagc tctcgccgca tgcgctgcga
     1261 ctgcgttggc gagatttccc gtggacgcgc aggagacagt gtcgaaaccg aactctatgg
     1321 cttttgatat cgcaacggaa accacccgat ctttgtatga gaaagtgggg tgattcactg
     1381 aatcatcttt gatgtaaagt tctttgaccc ccaatgcctg cccaagcctc cgggcgcgga
     1441 cgagcggcgt aaaaccggaa taaagaccca cctgcggttc ttcgtcgatg ggaagtagct
     1501 cgcgatagcg ccacaggttt ttgtcccggg aagtgatttt ttcacggctg atggattttt
     1561 ttatggcatc ataatcgtag acaatctcca gcgggccgaa gcaagtttca cacacgtgaa
     1621 gcggctcctt cggataattg tgcccgcatt ctctgcactg aagccctcta acgaagctca
     1681 tgattatccc tctcgtgatt tgagttttag gtgcttgggg cgatacttga ccctatggtt
     1741 tccccaccac aggcggtaca acgaacgtct cggcttacat cgaccgtatg aaacgtcatg
     1801 gattttgcgt cgtacgtcag taatcgatcc gtcaataatc cttcctcaaa gccggcgaga
     1861 tacttgatcg cttcagtggc ttggatcgaa cccacggtcc ctgcaacggc acccagaata
     1921 ccggattgct ggcaattgag tatttcttcg ggaggaggcg gttctctgaa gagacaacga
     1981 tagcaggcgc tttgccgcgg cacgaccgtc atcgtttgtc cctgcagccg gacgattcct
     2041 gcgtgagaaa aggcgatgtc catactcacg gcaatatcgt taaccaggaa tttggtggga
     2101 aaattgtcgc tgccgtcgat gataaagtca tagccggaaa ag
//
LOCUS       DASSWZ010000324         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_508111, whole genome shotgun sequence.
ACCESSION   DASSWZ010000324 DASSWZ010000000
VERSION     DASSWZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508111"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1102)
                     /locus_tag="VFN58_07810"
     CDS             complement(26..1102)
                     /locus_tag="VFN58_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET8917932.1"
                     /translation="MTGQLEEISAQKLRTVFNKETVILSLAALAGDASSRRYYRAKLA
                     GPATPSSVVIMELPSGSVLPLSSEELAIFKNPLDELPFLNVHRFLTRIGVKVPRLYDQ
                     SMDEGLLFLEDLGDLALWDRVQGLPPSEVLAWYQRAIDELLVLQIRGTKERDESCIAF
                     QQRFDFRLYMWEFDHFLEFGLEKRPGTNINQTSEEELRRVFVEIAQRLDGLVPCLSHR
                     DYHSWNLMIHDEAVAVIDFQDALLAPPQYDLASLLNDRITDSVIQPDLEEKLVDYYVK
                     KRLDMAREHIDRDEFTEMYLLSAIQRDLKVVGRFHYLDVVKGKTTYKRFIPPTVRRLK
                     RNFARLPQTERLIPILAEHYEEMR"
     gene            1159..2388
                     /locus_tag="VFN58_07815"
     CDS             1159..2388
                     /locus_tag="VFN58_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET8917933.1"
                     /translation="MINLAQGFLYFAVFLLIVAPLQLYLFKQVQSYIKEQVKEVRLVR
                     KLSFVLGFLFIVMQLPALWRGLFGLNISDPYSQLVRGLFTASTIWAVGSIGAAAIFLV
                     YNLFQRFNLLSRAPVAVTPDPARREFLKKGVGLAAAAPFMVSGYGALLERRKFEVDHF
                     TIPVPNLSSELNHLTVVQLSDIHVGPFMPPEELAEYVEAVNRLKPDIIALTGDFVTGS
                     ESEVVPCAESLAQLHARYGVFACMGNHDVYARAEDKLEHLFAEKGIETLRNNAKSIRI
                     AGSKVSMLGIDDLERGHSDLRRALTVCEREPGEVKVLLSHRPEIFPAAAKGDIDLVLS
                     GHYHGGQVKLSPAARSLSVARLLTPYAEGLFRLAPAGHGAGKTAHLFVSRGIGITGVP
                     IRINCPPQIAHLRLIKA"
BASE COUNT          537 a          636 c          678 g          626 t
ORIGIN      
        1 tggccggccg ccagcaccat cgccttcatc gcatttcctc gtagtgctcc gctagaatcg
       61 gaatgagcct ttccgtctgc ggcagtcggg cgaaatttcg tttcaatcga cgcactgtcg
      121 gcggaatgaa tcttttgtag gtagtcttcc ccttgacgac gtcgaggtag tggaagcgcc
      181 cgacgacttt caagtctctc tgaatcgcgg agagcaaata catctcggta aattcgtctc
      241 ggtcgatatg ttcccttgcc atatcgagtc tttttttgac gtagtagtcc acgagttttt
      301 cttcgagatc gggctggatg acgctgtcgg taattcggtc attgagaagc gaggctagat
      361 catactgcgg cggcgctaac agggcatcct ggaagtcgat cacggccaca gcttcgtcgt
      421 gaatcatgag attccagcta tgatagtcac ggtggctcaa gcagggaact aggccatcca
      481 gacgctgggc gatctcgaca aaaaccctcc tcaactcttc ctctgaagtt tgattgatgt
      541 ttgttcccgg ccgtttctct aagccgaact ccaggaaatg gtcaaactcc cacatgtaca
      601 ggcgaaaatc gaagcgctgt tgaaaggcta tgcaagattc gtcgcgctct ttggttccgc
      661 ggatctgcag cacgagcagc tcgtcgatgg ctctttggta ccacgccaag acttccgacg
      721 gtggcagacc ttgcactctg tcccatagag ctagatcgcc caggtcttcc aggaacaaaa
      781 gcccttcgtc catcgattgg tcgtagagtc tcggcacctt gacgccgatc cgtgtcaaga
      841 atcgatggac attgagaaaa ggcaactcgt caagtgggtt tttaaagatg gccaactctt
      901 cggaagaaag aggtaaaacg ctcccactcg ggagttccat gataacgacc gatgacgggg
      961 ttgctgggcc ggcgagctta gctcggtaat aacggcggct ggaagcgtct ccggccaggg
     1021 cggcaagcga gaggatcacc gtctccttgt tgaagaccgt gcgcagtttt tgtgcggaga
     1081 tttcttcaag ttgtccggtc actgttatgt ctctctttcg ttgccactcc tggaaatggc
     1141 cgttataatt gtaaagatat gatcaacttg gcgcagggat tcctatattt cgccgttttc
     1201 ttgctgattg tcgcacccct tcaattatat cttttcaagc aggtgcagag ctacataaaa
     1261 gaacaggtaa aagaagttag gttggtaaga aagttatcat ttgtcttggg tttcctattc
     1321 atcgttatgc aattgccagc gctttggcgt ggcctctttg gcttgaatat cagtgaccct
     1381 tattcccaat tggtccgagg attgtttact gcttccacta tctgggcggt aggttcaatc
     1441 ggagctgcgg cgattttcct ggtatataac ttgtttcagc ggtttaatct attatccagg
     1501 gccccggtag ccgtcacacc ggatccagca aggcgcgagt ttctgaaaaa aggtgtcggc
     1561 ctagcagcag ccgcgccttt tatggtttcc ggttacggtg cgctattgga aaggcgcaaa
     1621 tttgaagtgg atcattttac catcccagtg ccgaatcttt catcagagct gaaccatttg
     1681 actgtagttc agttgagcga tatccacgtc ggccctttta tgcccccgga agaacttgcg
     1741 gagtatgtgg aagcagtgaa ccggctcaag cccgatatca tagcgcttac cggtgacttt
     1801 gttacgggta gcgagagcga ggtagttccc tgtgctgagt ctctcgcgca attgcatgcg
     1861 cgttacggtg tgtttgcatg tatggggaat cacgatgttt acgcccgcgc ggaggataag
     1921 ctcgaacacc tgtttgccga gaaggggatt gagactctgc gtaacaatgc aaaaagcatc
     1981 cggattgcgg gctccaaagt ttccatgctt gggatcgacg atcttgagcg agggcactcc
     2041 gatttacgcc gcgcgctcac ggtttgtgaa agggagccgg gagaagtaaa ggttctgttg
     2101 tcccatcggc cggagatctt tcccgcagca gccaaaggcg acatcgatct ggtgctttcc
     2161 ggccactacc atggcgggca agtgaagctg agcccagctg cgcgaagtct ttccgtcgcc
     2221 cgactactga cgccttatgc tgaggggctt ttccggcttg cgccggcggg ccacggcgct
     2281 gggaaaaccg cgcacctgtt cgtcagtcgt ggcataggaa tcaccggggt gccgatcagg
     2341 atcaattgcc ctccgcagat cgcgcacctg agattgatca aggcgtgagt ctgagcagcc
     2401 ggcaaacaga gctttgatgc gggccgacgc catcggcctt cttaaacctc tcaactgccg
     2461 ccaaagccga tcgtgac
//
LOCUS       DASSWZ010000325         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_380627, whole genome shotgun sequence.
ACCESSION   DASSWZ010000325 DASSWZ010000000
VERSION     DASSWZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380627"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFN58_07820"
     CDS             complement(<1..489)
                     /locus_tag="VFN58_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET8917934.1"
                     /translation="MRILRLLSIAFLAVCGLPQEQLAGTPPDLKIEVLVRGLDTPWAI
                     DFAPNGRIFITERPGRIRVVEEGRLLPEPWITLEVAAAGEAGLMGLALDPQFGQNRFA
                     YVAYTYRAANGRMQNRLVRLREDSKTGKGSLDKVLIDNVAGYNNHDGGRVKFGPEGKL
                     YWT"
     gene            complement(538..2067)
                     /locus_tag="VFN58_07825"
     CDS             complement(538..2067)
                     /locus_tag="VFN58_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET8917935.1"
                     /translation="MHRIVFGLLIFSFLTVEPSILSAQDNIRDLVVKIHAVHHTPDVL
                     RPWTKNSPQQVKGSGVVIEGKRILTNAHVVRYASQIYVQPNQSAERIPARVEAMTPSM
                     DLAVLKLDDESFFDNRGTLPFAEELPRVKDTVNVYGYPTGGSELSVTQGIVSRIEYTD
                     YYFQTSGLRIQVDAALNFGNSGGPAVSDGKLVGLVFSLIQNAQSIGYLIPVEEIQLFL
                     ADITDGKYDGKPQLYDYLQTVENEALRQRLGLPAGVNGVMVTEPYRQDPNYPLKEWDV
                     ITQIGNTPIDSEGKVAVRYDLRLSALYLVQKLAKDGYIDVTVFRDGKLNPMKLPVQSH
                     RELVMPYLLNATPRYFILGPLVFSQATQDYIERASAQRPMPASQQNSPLVTRRYDKPR
                     FDGEELVVVTSPMFPHRITKGYDDPERFVLSEVNGQRVKSLQHLVQLLRDDTQSQITL
                     KFARASRRTHETMVFNRAELLESTAKVIEENGIRYPYSFDLRPLWEKSLTVMPKSAAG
                     S"
     gene            2400..2708
                     /locus_tag="VFN58_07830"
     CDS             2400..2708
                     /locus_tag="VFN58_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET8917936.1"
                     /translation="MWYLVLRRLVKPREEWTVTLDQHLVWMKQQHEGGKILFSGPTPD
                     RKLGIYVIRAGSRQEAEKIATSDPYTAAGFTAFDLIEWEVHQIMGAGPFTAAGLQAHK
                     "
     gene            2744..>3096
                     /locus_tag="VFN58_07835"
     CDS             2744..>3096
                     /locus_tag="VFN58_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917937.1"
                     /translation="MNARNSRKCVIVVSLCLLLSFSAPAGAQSDWKKQWETTIEAGKK
                     EGEVVIYGPHNPMYQQLWAVFQKSFSEIKFNFVPGKGADHYQRIVAERRAGKYLADLV
                     MGGSSSFASFPPGTL"
BASE COUNT          726 a          783 c          864 g          723 t
ORIGIN      
        1 tgtccagtaa agttttccct caggaccaaa ttttactcgg ccgccgtcgt gattgttgta
       61 gcctgcgacg ttatcgataa ggactttgtc caaactgcct ttgccggtct tcgaatcttc
      121 gcgcagccga acaagacgat tttgcatccg gccgttggcg gcacggtatg tataagccac
      181 ataagcaaag cggttttggc caaattgcgg gtcgagagct aaacccatga ggcccgcttc
      241 acccgcggcc gcgacctcta acgttatcca aggttcaggt aatagtcgcc cctcctcaac
      301 gactcggatg cgaccgggcc gttccgtaat aaagattctg ccattgggtg caaagtcgat
      361 ggcccacggt gtgtccaagc cgcggaccag gacttcaatc tttaaatccg gcggcgtgcc
      421 ggcgagttgt tcttggggta gaccgcagac ggcgagaaaa gcgattgaaa gcaagcgcag
      481 gatgcgcatg aatttagcga tttaaagtcc aaaacttcgc gagaatcccc ggcacgatca
      541 tgagccagcc gccgacttcg gcataacggt cagagacttt tcccaaagcg gccggagatc
      601 aaaggagtag ggataacgaa tgccgttttc ctcgatgacc ttggcagtgg attctaaaag
      661 ttcggcgcga ttaaagacca tggtttcgtg agtacggcgc gaggctctgg cgaatttgag
      721 ggtgatctgg gactgcgtgt cgtcacgcag aagctggacc aggtgttgca gactttttac
      781 acgttgaccg ttgacctcgc tgagcacgaa gcgctcgggg tcgtcatagc ccttggtgat
      841 gcgatgggga aacattggcg aggtaaccac gaccagctct tcaccatcaa aacggggctt
      901 gtcgtagcgg cgcgtcacca gcgggctatt ttgctgactt gcgggcatcg gcctttgggc
      961 gctcgctcgc tcgatataat cctgagtcgc ttgggaaaag accaggggcc cgagaatgaa
     1021 atagcgcgga gtcgcgttta atagataggg catgaccagc tctcggtgcg attggaccgg
     1081 cagcttcatc gggttgagct tgccgtcgcg aaaaaccgtg acgtcaatat atccgtcctt
     1141 ggccagcttc tggaccaggt atagtgccga aaggcgcaga tcgtaacgga ccgcaacttt
     1201 gccttcgctg tcgatgggtg tgttgccgat ctgggtgatg acatcccact ccttgagcgg
     1261 gtagtttggg tcctgccggt agggttccgt gaccatgact ccattcacgc cggcgggaag
     1321 cccgagtctt tggcgtaacg cttcattttc gaccgtttga agatagtcgt aaagttgcgg
     1381 tttaccgtca tacttgccat cggtgatatc ggccaagaat agctgaattt cttcgacggg
     1441 gatcagataa ccgatacttt gggcgttttg tatcagacta aaaaccagac ccaccaattt
     1501 tccatccgat accgccggtc cgccgctgtt gccgaagttg agcgcggcgt ccacctgaat
     1561 ccttaacccg ctggtttgaa aataataatc ggtgtattcg atgcgagaga cgatgccttg
     1621 agtaacggaa agctccgacc cgccggttgg atagccgtag acattaacgg tatctttgac
     1681 ccgcggcaac tcctctgcga acggaagcgt tcctcgatta tcgaagaatg attcgtcatc
     1741 aagctttaac acggccagat ccatcgacgg agtcatcgct tccacgcgcg ccgggatacg
     1801 ttcggcggac tggttgggtt ggacgtaaat ctggctggca taacgcacca cgtgcgcgtt
     1861 ggtgaggatc cgtttaccct cgatgaccac gcccgagccc ttcacttgct gcgggctgtt
     1921 tttggtccac ggacgcaaaa cgtcgggggt gtgatgcact gcgtggatct tgacgaccaa
     1981 atcccgaata ttgtcctggg cactcaatat agacggttcg accgttagaa aactgaatat
     2041 caataagccg aaaacgattc gatgcataag gttgcctcgt ttctgtggcg cgatgcgcgg
     2101 aacatattat atacaaaaac gggcagagtt aaacggtacc tccctagtga tctcctttga
     2161 ctgtgcatgc caggttttgt attccttccg agtcaccgtt cccatgtcgc ctcaagctat
     2221 ggctgatttg gcttcagtat tccacacctc ttgggtcacg tataggaaat atgaccttga
     2281 aaaagttctt gacacgcgaa gcaggcaatc atatccctgc gtcatagagt ctgacgtttc
     2341 gagtctcgcg atcagccgaa acctgaaacc cgaaaccaca aaattgttgg gaggtaatca
     2401 tgtggtactt agtgcttagg cgtcttgtga aaccgcgcga agagtggacc gtgaccctgg
     2461 atcaacatct ggtctggatg aaacaacagc atgaaggtgg aaaaatcctc ttctcgggtc
     2521 cgaccccgga ccggaaactc ggcatttatg ttatccgcgc tggatcgcgg caagaggcgg
     2581 agaagatcgc cacaagcgat ccctacacgg cggcaggctt taccgctttc gacttgatcg
     2641 agtgggaagt gcaccagatc atgggcgcgg gaccatttac ggcggcggga ttacaagctc
     2701 acaaataaat ggcaaacaag ctttgtttgt gaggaagcca gatatgaacg ctcgaaactc
     2761 taggaagtgc gtgatcgttg tttcattgtg cctgctgtta agcttttcag caccggccgg
     2821 cgcgcagtcc gactggaaga agcagtggga aacaaccatt gaggctggga agaaagaagg
     2881 ggaggtggtg atttacggac ctcacaaccc gatgtaccag caactctggg ctgtgtttca
     2941 gaaaagcttt tcagaaatca agttcaattt cgttccaggc aaaggagcag accactatca
     3001 acgtattgtt gcggaaagac gggcgggaaa atatttagcg gacctcgtca tgggaggatc
     3061 ttcgagtttt gcgtcctttc cgccggggac cctgga
//
LOCUS       DASSWZ010000326         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_706376, whole genome shotgun sequence.
ACCESSION   DASSWZ010000326 DASSWZ010000000
VERSION     DASSWZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706376"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFN58_07840"
     CDS             complement(<1..698)
                     /locus_tag="VFN58_07840"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET8917938.1"
                     /translation="MAFSDLRTFIEAAADIGEAKTVEGAHWNLEIGCLTELMAEQEGP
                     LLLFDSIAGYPKGFRIATNVLATARRFALALGLPTELPKLEILKAWREKTRNLKPFSP
                     VEVSFGPVTENILAGDRIDLGIFPTPKWHEHDGGRYIGTGDMVITKDPDSGWINVGTY
                     RACVVDKNRLTLWIIDHKHGKQIARKYWQAKHACPVAIVLGCEPATWTAAPSAAKAGV
                     SEYDYAGALRGEPV"
     gene            complement(855..1598)
                     /locus_tag="VFN58_07845"
     CDS             complement(855..1598)
                     /locus_tag="VFN58_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917939.1"
                     /translation="MFKCTEREAEDLIAEKKAKLEGRWKQTVDRTRFMDELREGKLKK
                     ETIQLYYKNWGAFVPVINSIYTSAFYKHLWFFVKNVDLMEVYTQKVLDEFGHPCPPGH
                     IQILIGTGQALGLSAEEVLLTPMLPEARALPDFQRTLMVDGQIQEYWFSVLWEHAFGE
                     TCLDWFRALTTHYGLTTEQASYFSKHHEADTMDHLDRKAHGAVTQTVLVRLLQQGIIE
                     RPGYSAEYCAVTPADLNAMMMDGCYKATH"
     gene            1766..2122
                     /locus_tag="VFN58_07850"
     CDS             1766..2122
                     /locus_tag="VFN58_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8917940.1"
                     /translation="MDFVNLNEMMATKKRNIRLFGTDNFRSWMLYFVPGDGTDMHYHA
                     NPETFLVLSGSGVVKGIKGEERPIQKNEVVFFAAKDYYQITNTGTEPLVLFGNRSEGF
                     GGPHIKSDGTDRNVRA"
     gene            2213..2551
                     /locus_tag="VFN58_07855"
     CDS             2213..2551
                     /locus_tag="VFN58_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET8917941.1"
                     /translation="MPIYEYQCQKCGTFEVTQRITEKPLGKCPTCKGKVKKLISNTSF
                     QLKGTGWYVTDYARKDNNKGAGKGENGAKPSAESKSDSKSDSKGESKTESSSTGSPKS
                     SSTSSSSTSA"
BASE COUNT          627 a          701 c          718 g          634 t
ORIGIN      
        1 tgcaccggtt cgccgcgcag cgccccggca tagtcgtatt cgctcacccc ggctttggcc
       61 gccgacggcg ccgccgtcca agtcgcaggc tcgcagccca agacaatcgc gacgggacag
      121 gcgtgcttcg cttgccaata ttttcgcgcg atctgtttgc cgtgcttatg gtcaatgatc
      181 cacaatgtga gtcggttctt atcaacgacg caggctcgat aggtgccaac gttgatccaa
      241 ccactgtccg ggtctttggt gatgaccatg tcgccggtac caatatagcg cccgccgtcg
      301 tgctcgtgcc atttcggcgt ggggaatatt ccgagatcga tgcggtcgcc tgcaagaata
      361 ttttcggtca ccgggccgaa agaaacttcc acgggtgaaa acggctttag gttgcgcgtc
      421 ttttcgcgcc acgctttgag aatttccaac tttggcagtt ccgtgggaag acccagagcc
      481 aaggcaaaac gccgggcggt ggcgagcaca ttagttgcga tgcgaaagcc tttcggatag
      541 ccggctatgc tgtcaaacag gagcagcgga ccttcctgtt cggccattag ctctgtcaaa
      601 cagccgatct ccagattcca gtgagcaccc tcaacggttt tggcttcgcc aatgtcggcc
      661 gcggcttcga tgaaggttcg aagatcagag aaggccattg aatttatctc cagcgctatg
      721 ccctccccgc tggggaaagc gaaccgggct gctcacggtc ctgtttctgc tcgcattcgg
      781 gatggtccaa accttgagtc cttgattgag ctcttcaaga gacatagcgt catttgcaat
      841 ccgaaaggga aaggtcaatg cgtcgccttg tagcagccgt ccatcatcat tgcgttaaga
      901 tccgctggcg tcactgcgca atactccgcc gaatagcccg ggcgttctat gattccttgc
      961 tggagaaggc gtactaatac ggtttgtgtg accgcaccgt gggcttttcg gtccaagtga
     1021 tccatcgtgt cggcttcgtg atgtttggag aaatagcttg cctgctccgt agttagaccg
     1081 taatgagtgg tcagcgcgcg gaaccaatcc aaacaggttt ccccaaacgc gtgctcccag
     1141 aggacggaga accaatattc ctgaatctgg ccgtccacca tcaatgtccg ttggaaatcg
     1201 ggcagcgccc gtgcttctgg cagcatcgga gtcagcaata cttcctcggc ggagagcccc
     1261 aaggcctgtc ccgtgccgat aaggatctga atgtgaccgg gagggcaggg atggccaaac
     1321 tcatcgagta ccttttgggt atagacctcc atcaggtcga cattctttac gaagaaccac
     1381 aggtgcttat aaaaggccga tgtgtagatg gagttgatga ccggcacgaa ggctccccaa
     1441 ttcttgtaat acaattgaat ggtttccttc ttgagcttcc cttcccgcag ctcatccatg
     1501 aagcgagttc tgtcgacggt ttgcttccaa cggccttcca acttagcttt tttctccgcg
     1561 atcaaatcct cggcttctcg ttccgtacac ttgaacatcc taggtctccc ttttgaggcg
     1621 atgatctgta gatatgtgag gaacctcctg gctgtcaagt aaagtcaggc cggccgcgcc
     1681 tcgcaatgtc gagttggagg agatgggcgg gttgccattt ggcgagcaat cgagataaga
     1741 tcgtcaaaat atagagaggt atattatgga ttttgtgaat ctcaatgaaa tgatggcgac
     1801 gaagaaacgg aatattcgtc tctttggaac ggacaatttc cgttcctgga tgctctactt
     1861 tgttccgggt gacgggacag atatgcacta tcatgccaac ccggagacct ttttggtcct
     1921 ctcgggaagc ggcgtggtca agggaatcaa gggcgaggag aggccgattc aaaagaacga
     1981 ggtagtcttt ttcgccgcca aagactatta ccagatcacc aatacgggga ccgaaccttt
     2041 ggtgctgttt ggtaatcggt cggagggttt tggcggcccg cacatcaaat ccgacggaac
     2101 ggatcgcaac gtcagagctt agtctattgc ggtcggccgg agggcatcaa ttgacaaccg
     2161 gcgggcgagt ggttaatttt attaagcaaa ccgtgcaact agaggatttt ccatgcctat
     2221 ctacgagtat caatgccaga aatgcggcac ctttgaagta acacagcgga tcacggaaaa
     2281 gccattaggc aaatgtccta cgtgtaaggg aaaggtcaag aaactgatat ccaatacctc
     2341 ctttcaattg aagggtaccg gctggtacgt cacggactat gcccgcaaag acaataataa
     2401 gggagccggg aagggtgaaa acggtgccaa gccctccgca gagtccaaaa gcgattcaaa
     2461 gagcgattcc aaaggcgagt ccaaaactga aagttcttcg accggcagcc caaagtcctc
     2521 ttccacatca tcgagctcga cgtcagctta atagttgtca accatcagcc tagaatagct
     2581 cgggttcctt tccactcaaa tccatttaca gtgaaacggg cggcgaagct gccgctcgtg
     2641 ttttaatcct gttacggaaa acattttcct gcgtgattcg
//
LOCUS       DASSWZ010000327         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_791406, whole genome shotgun sequence.
ACCESSION   DASSWZ010000327 DASSWZ010000000
VERSION     DASSWZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791406"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFN58_07860"
     CDS             complement(<1..361)
                     /locus_tag="VFN58_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8917942.1"
                     /translation="MNFDLTEEQKLLKQTIRDFAETQIAPGAAARDEAARFPTELIPK
                     MAELGLFGIMIPQEYDGAGLDTLSAAIIGEELARVDAAVALIVASHNSLCAAHIHNFG
                     NEMQKQKYLPPLAHGEKL"
     gene            complement(409..870)
                     /locus_tag="VFN58_07865"
     CDS             complement(409..870)
                     /locus_tag="VFN58_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET8917943.1"
                     /translation="MVRRRSGRTNNRKPPVRKLRVLIAKVGLDGHDRGVKIVARALRD
                     AGMEVIYLGIHNTPEEIASSALQEDVDAIGLSIHSAAHMTLFTEIFGLLRKKKARDIA
                     VFGGGIVPPEDIRSLKKMGVKEIFTPGAPLEEIVNFVNSLPPRKRNASLKQ"
     gene            complement(978..2573)
                     /locus_tag="VFN58_07870"
     CDS             complement(978..2573)
                     /locus_tag="VFN58_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET8917944.1"
                     /translation="MKSMEKHFRNLSEIEIRSVYSPEDIKDFDYRRDLGSPGEFPFTR
                     GIYPTMYRERLWTMRQFAGFGIAEESNKRYRYLLKQGNTGLSVAFDMPTLMGYDSDDP
                     RSRGEVGRCGVAIDSLSDMEALFRDIPLDQVSVSMTINGPAIVLFAMYLVVAERQGVG
                     FRDLQGTLQNDILKEYIAQKEWICPPAPSLKIINDIVRFCVESAPRYHPISISGYHIR
                     EAGSTAVQELAFTLYDGLTYVSSAIEAGLKVDDFAPRLSFFFNAHNDFFEEIAKYRAA
                     RRLWAREMKTRFRPENLASLALRFHTQTAGCALTAQQPYNNVVRVALQALAAVLGGTQ
                     SLHTNSLDETLALPTEEAVKIALRTQQIIAHESGVANTVDPLAGSYYLEWLTNRMESE
                     AKAYFDKLDEIGGMIQAIQRGFPQSEIHRVAVAYQKETDNRKRIIVGVNAYTEPEERR
                     ISILKIKGEVEKKQVQRLKQRKKNRNLRKVASVLSQLAGVAEANGDLMPAVMEAVRAQ
                     STVGEVCEVFRRVYGEYREAVSL"
     gene            complement(2548..>3096)
                     /locus_tag="VFN58_07875"
     CDS             complement(2548..>3096)
                     /locus_tag="VFN58_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET8917945.1"
                     /translation="SLLFEDCRVPGENLLGTEGEGFAIALATLDGGRIGIAAQALGIA
                     QGCLEESVAYAQQRRQFGRAIAEFEAIQWMLADMATEIDAARLLTYRAAWLAEQGRTF
                     TKEAAMAKLFASEAANRAAYKAIQIFGGYGYTKEFAVERFFRDARITTLYEGTSEIQR
                     LVIARRLIHGEEIAHEIHGETF"
BASE COUNT          643 a          889 c          816 g          748 t
ORIGIN      
        1 cgagcttctc gccatgcgct aaaggaggca ggtatttttg cttctgcatt tcgttgccaa
       61 agttatgaat gtgagccgcg caaagggaat tatgggaggc gacaatcaac gctaccgcgg
      121 cgtccacacg agcgagctcc tcgccgatga tcgcggcgct aagggtatct aaaccagccc
      181 cgtcgtattc ctgcggaatc atgatgccaa agaggcctag ctcggccatc ttgggaatca
      241 gctccgtggg aaaacgcgct gcttcgtcgc gcgctgccgc tcccggcgca atctgcgtct
      301 cggcaaaatc gcggatcgtc tgcttgagaa gcttttgctc ctccgtgaga tcaaaattca
      361 taacgctctt cgttcacttc cgcgcttggc caacacttgg aagcggattc attgctttag
      421 cgacgcattt cttttccttg gcggaagcga gtttacaaaa ttaacgatct cttcgagggg
      481 cgcgcctggt gtaaagatct ccttgactcc catcttcttg agactgcgaa tgtcctccgg
      541 aggaacaatt cctcccccaa atactgcgat gtcccgtgcc ttttttttcc tgaggagccc
      601 gaatatctcg gtgaacagag tcatatgggc ggcggagtgg atggaaagac cgatggcgtc
      661 cacgtcttct tggagcgccg agctagcgat ctcttccgga gtgttgtgga tgccgagata
      721 gatcacctcc atgccggcat ctcgcagcgc ccgggcgacg atcttaaccc cacggtcatg
      781 accgtcgaga ccgaccttgg caattaaaac tcggagcttc cgaactggcg gctttcgatt
      841 gttcgtccgg cctgaacgtc gtctgaccat gtctcagttg tcgctggaat attggagtgg
      901 tggagtgatg aagtactgga ttattgcaga attccgaccc cacccaacac tccattgctc
      961 cactactctg gttttcgtca cagactcacc gcctcgcgat actctccgta aaccctgcgg
     1021 aacacttcgc agacttcccc gacagtggat tgcgctcgta ccgcttccat gaccgccggc
     1081 atgagatcgc cgtttgcttc agcaaccccc gcaagttgac tgagcacgga agctactttc
     1141 cttaagttgc gatttttctt tctttgcttc agtctctgaa cctgcttctt ctccacttct
     1201 ccttttattt tgagaatcga gattcggcgc tcttcaggct ctgtgtaagc gttcacacct
     1261 acgatgattc gcttccggtt atccgtctct ttctgatagg cgaccgcgac gcggtgaatc
     1321 tcgctctggg gaaagccgcg ctggatcgct tggatcatgc ctcctatctc atccagcttg
     1381 tcaaagtaag ccttcgcttc ggattccatc cggttggtca accactccag ataataggag
     1441 ccagccaatg gatcgacggt gttcgccacg ccggactcat gggcgatgat ctgttgagtc
     1501 cgaagggcga tcttcaccgc ttcctctgtg ggcaacgcca gcgtttcatc gagggagttc
     1561 gtgtgtaacg attgggttcc gcccaaaact gcggcgagcg cttgtagcgc tacccgcacg
     1621 acgttgttgt aaggctgctg cgcagttaga gcgcatccgg cggtttgggt atgaaagcgc
     1681 agagccaggg acgccaagtt ctctggtcgg aaccgcgttt tcatctcgcg cgcccacaga
     1741 cggcgcgcgg cgcgatattt ggcgatctct tcgaagaagt cgttatgagc gttgaagaag
     1801 aaagaaagtc tgggagcgaa atcgtctacc ttcaatcccg cttcgatggc cgagctcaca
     1861 taggtcagcc cgtcatacaa agtaaaagcg agctcttgaa ccgcagtgga gccggcttcc
     1921 cggatatggt agccgctgat gctaatggga tgatagcgcg gtgccgattc gacgcaaaag
     1981 cgaacgatgt cgttgatgat tttgagcgac ggagctggtg ggcagatcca ttccttctgc
     2041 gcgatatatt ctttcaggat atcgttctgt agcgttcctt ggagatctct aaagccaacc
     2101 ccctgccgtt cagccacaac cagatacatc gcgaaaagaa caatcgcggg accgttgatg
     2161 gtcatcgata cgctcacctg atcgagagga atgtcgcgaa acagcgcttc catgtccgac
     2221 agcgagtcga ttgccacgcc acatcttccc acctcgcctc tcgatcgagg atcgtcggag
     2281 tcgtaaccca tgagagtcgg catgtcgaag gcaacggaaa gccctgtgtt gccctgctta
     2341 agtaggtagc ggtaacgctt gttactttcc tcggcgatcc cgaagccagc aaactggcgc
     2401 atggtccaga gtctttcccg atacatggta ggataaatcc cgcgggtgaa gggaaattct
     2461 ccggggctgc caaggtcgcg ccggtagtcg aagtccttta tgtcctccgg actataaacg
     2521 gatcgaatct caatctcgga gagattccta aaatgtttct ccatggattt catgggcgat
     2581 ctcctcgccg tgaatcaagc ggcgcgctat gaccaggcgt tgaatctcag aggtcccttc
     2641 atagagtgtt gtgatccgcg cgtcccgaaa gaagcgctcg acggcgaatt ccttcgtgta
     2701 gccatagccg ccaaagatct gaatcgcctt ataggctgcc cggttggcgg cctccgacgc
     2761 gaacagtttc gccatggctg cctctttggt gaatgtcctt ccctgctctg ctagccaagc
     2821 tgcgcggtac gtcagcagcc gtgccgcatc gatctccgtt gccatgtcgg ccagcatcca
     2881 ctgaatggct tcaaattcgg ctatggcacg gccaaactga cggcgctgct gcgcgtatgc
     2941 cacggattct tccagacatc cctgcgcgat gccgagcgct tgagcggcga ttcctatccg
     3001 gcctccatcc aatgtcgcca gcgctatcgc gaaaccttct ccttctgttc ctaaaaggtt
     3061 ttcacccgga acccggcaat cctcgaaaag taaact
//
LOCUS       DASSWZ010000328         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_904784, whole genome shotgun sequence.
ACCESSION   DASSWZ010000328 DASSWZ010000000
VERSION     DASSWZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904784"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1284
                     /locus_tag="VFN58_07880"
     CDS             175..1284
                     /locus_tag="VFN58_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HET8917946.1"
                     /translation="MPHTDRSRLMMLMTVAALTLWGGGAYTQDEDQPTNDLPNPYQTI
                     APWGKLPEGRTWGALNAVAIDNDGESVWVADRCGANPDTPPGASAFQYDSCAGSTLPP
                     ILKFDSSGSLVKSFGAGMFIFPHKIYMDRDGNVWVADQRSANERELTKSPGEKGKGHV
                     VVKFSPDGKVLLTLGKPGVAGNPPDALTQPTSVVMAPNGDLFVSEGHNQSPQAPPETV
                     SRISKFTKDGKFIKSFGKLGSGPGEFRGPHDLAMDAQGRLFVADRGNMRIQILDQDGK
                     YLGEWKQFSRPSGVYIRNDMIYVADSESNGVATHPGWKRGIRIGSLKDGKVLYRIPDP
                     LEMKGTSAAEGIAVDVKGNVYGGEVGPRQVAKHVK"
     gene            1468..2436
                     /locus_tag="VFN58_07885"
     CDS             1468..2436
                     /locus_tag="VFN58_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET8917947.1"
                     /translation="MITANDLRGIMAMMPAFTTKDGDRPDAEDTVNTAELTRAVDQII
                     RDGGANVITTTGSFGEFHTLLWEEHKKLIEATVATVKKRVPVFIGCTNLNPREAMRQA
                     RFAQEAGADGLLLGVPFYYQATVDNAVQFYHDIADAFPKLGVMIYHNPTHHRVTIPVG
                     AFKKITQKPNIVGMKDSHRTPLQFIELMNTVQGKISVFVNQTQAYPYVMLGASGCWSF
                     NVWMGPSPVTRLRDACASQDWETAKQICLDLDGVGKVGPNIGNLSWRENVFKLSVNEA
                     GYCTAGPLRAPWRIVPQEVIDNSKKIAAYWKTLCERYPAEQLAKSA"
     gene            complement(2609..>2872)
                     /locus_tag="VFN58_07890"
     CDS             complement(2609..>2872)
                     /locus_tag="VFN58_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917948.1"
                     /translation="SIPDVAEGWQHPITREDSAIWDFPTLSQLVNGEHPGRTKNDAIS
                     CFCNNVGLGLQFAAVGSEVLARAREARVGREIPTEWFLESVHP"
BASE COUNT          689 a          810 c          801 g          572 t
ORIGIN      
        1 ctcgccttga gacgctcgaa gacaccctgc tcaacctcga gaagctcggc gatgcaagaa
       61 aaattggcag gctgctcacg gcctgattga tcaactcagg tcgaagtccc gaagacaaga
      121 ccgcgccctc gcttgggtcg aatctcgtta aagttgagag aaaggagtgc tccgatgccg
      181 catacagatc gatccaggtt gatgatgctc atgaccgtcg ccgcgctgac attatggggt
      241 ggcggagcct acactcagga tgaagaccag cccaccaacg acctgcccaa tccgtaccaa
      301 accatcgcac cgtggggaaa acttcccgag ggaagaacgt ggggagcact gaacgccgtc
      361 gcgatcgata acgacggcga atccgtatgg gttgcggacc gatgcggcgc gaatcccgat
      421 accccaccag gggcttctgc cttccagtac gacagctgcg ccggctcaac tctgccgccg
      481 atcctcaagt ttgattcatc cggcagcctg gtgaaaagct ttggcgctgg gatgtttatt
      541 ttcccgcaca agatctatat ggatcgtgac ggcaacgtgt gggtggctga ccaaagatct
      601 gcaaacgagc gagaactcac gaagtccccc ggagaaaaag gaaagggtca cgtcgttgtt
      661 aagttcagtc cggacggcaa ggtcctgttg acgctcggca agcctggggt ggccggaaat
      721 ccaccggacg ctttgaccca gccgaccagt gttgtgatgg ctccgaatgg cgaccttttt
      781 gtctcagagg gccacaacca gagcccgcaa gctccgccgg agaccgtttc acgcatctcc
      841 aagttcacga aggacggcaa gttcatcaag tcctttggta agttgggatc ggggccggga
      901 gaattcaggg ggcctcacga ccttgccatg gatgcgcagg gtcgactttt cgttgccgac
      961 cgggggaaca tgcgcatcca gatcctcgat caagacggga agtacctcgg agagtggaag
     1021 caattcagtc gccccagcgg tgtttacatc cggaacgaca tgatctatgt ggccgattcc
     1081 gaatccaatg gggttgccac acacccggga tggaagagag gtatccggat cggaagcctc
     1141 aaagatggta aggtcctgta ccgtattcct gacccgctcg aaatgaaggg aacaagtgcc
     1201 gcggagggca tagcggtgga tgtgaagggc aacgtgtacg gcggcgaggt cggcccgcga
     1261 caagtggcga aacacgtcaa ataattggaa cccgagttcg tgcgggaact tagtttaccg
     1321 gcgcgtgttc atcatcaaca atttcaacgc gacgcttgga aatcccatca gccgctcgaa
     1381 ctgcttgact cccccttccc gctggaggag aatccggctc aagcttgaac gacactgcga
     1441 tttgaaaaat cataaaggag accctcaatg atcacagcaa acgatttaag aggcattatg
     1501 gcgatgatgc cggcgtttac gaccaaggac ggcgaccggc cggatgcgga agataccgtt
     1561 aataccgctg agttaacgcg cgcggtggat caaattatcc gcgacggcgg cgccaatgtc
     1621 atcacgacca ctggcagctt cggcgaattt cacacactgc tttgggaaga gcacaagaag
     1681 ttgatcgagg ctacagtggc tacggtcaaa aagcgagtgc cggtgttcat cggctgtacg
     1741 aacttgaatc cccgcgaggc catgcgacag gcgcgattcg cccaagaggc aggcgcggac
     1801 ggcttgctgt taggtgtgcc tttttactat caagccacgg tggacaacgc cgtgcagttc
     1861 taccatgata tcgccgatgc gtttcccaag ctgggcgtca tgatctacca caacccaacc
     1921 catcaccggg tcacaattcc cgtcggagca ttcaagaaga taacacagaa gcctaatatc
     1981 gtgggcatga aggacagtca ccgcactccc ctccagttca tcgagctaat gaataccgtg
     2041 caaggcaaga tcagcgtctt cgtcaatcag acacaggcgt atccctatgt catgctcgga
     2101 gcctcaggct gctggtcgtt taacgtctgg atgggtccgt cgcctgtgac ccgtttgcgc
     2161 gacgcctgcg catcacagga ttgggaaacc gccaagcaga tctgtctgga cttggatggt
     2221 gtcggcaagg tcggtcccaa catcggcaac ctgtcctggc gcgaaaacgt tttcaaactc
     2281 tccgtcaacg aagccggata ctgcaccgcg ggaccgctgc gcgctccctg gcgcattgtg
     2341 ccccaagaag tcattgacaa cagtaagaaa atcgccgcct attggaaaac cttgtgcgaa
     2401 agatatccgg cagagcaatt ggcgaagagc gcgtaagcaa tttatgacag tctcgagtct
     2461 caggtcccgg gtctcgagtt agaaaacgcc aggcttttca ggtgcagtgg agacttggag
     2521 cactagcagt cgaaacccat tgctttgcta tccccgtttt ccaatcctcc accccaggac
     2581 ccgaaacccg caactcgaaa ctgcatgctt acggatgcac agattccagg aaccattccg
     2641 tgggtatttc ccgcccgact ctggcctctc tcgcacgcgc caaaacttca ctcccgaccg
     2701 cggcgaactg caacccaagg ccgacattgt tgcagaagca acttatcgca tcgtttttcg
     2761 ttcggccggg atgttctccg ttgacgagct gagatagtgt tggaaaatcc caaatagccg
     2821 aatcttcccg tgtgatcggg tgttgccagc cctcggcgac gtcgggaatc ga
//
LOCUS       DASSWZ010000329         2882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_522578, whole genome shotgun sequence.
ACCESSION   DASSWZ010000329 DASSWZ010000000
VERSION     DASSWZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522578"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFN58_07895"
     CDS             complement(<1..231)
                     /locus_tag="VFN58_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917949.1"
                     /translation="MRKKFFPGMLSALVLLAVICWAGVVAAQDFYKGKTIRLIVATTP
                     GGGFDAYSRMLARHIGKHISGNPTFVVENMPGA"
     gene            847..1728
                     /locus_tag="VFN58_07900"
     CDS             847..1728
                     /locus_tag="VFN58_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917950.1"
                     /translation="MDFRHRPRIIVNRFSAPVIVVPFQPFGYYPYCDSFYFGAYGCPS
                     PYTPFAPVYVPVPVSSYSGENPYADDNSVSFPADIADYTPSSGTTDPAAVSPESFERQ
                     GEYAFRARDYMAANRDWQHAVVDDPSSGALAMKLALAMFAVGKYREAAGTTQQVLMLL
                     PQEKWGQAVSDYKKLYANPQDYLDQLKNLAKAAADKPNDPALRFLLGFHYGYSGRVAD
                     AVRELNKLVQLEPKDQLGRKLRDLMADKTYKDNNSAIATAPSDIYVRKDSNGTLYFSN
                     IPADLNYQPATWHRTQY"
     gene            complement(1903..>2882)
                     /locus_tag="VFN58_07905"
     CDS             complement(1903..>2882)
                     /locus_tag="VFN58_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8917951.1"
                     /translation="PESDPRFEKLAKEFYPKAKQEGALVVYTVWDVDHIRSLSSAFSK
                     RFPGINTSYWQATRSEVTTRTLTEYQGNQATVDVILTEAPLVLHAAGATEPYQTVQGN
                     SLIVNDAIAPVVSLQIQALAYNTKKLKREEAPKSWEDVTNPRYKGIVALDDPMRAGPL
                     SSMLAAFKDDWKDDARWANFIRGLKSLNVSIHKSTSAMFRLVVAGEYSIVMPALFHDV
                     VREKEIGSPIDFARGAGPVVSPQQAAIYVKAPHPTAAKLFAEWLISPEGQSAIDAVGR
                     ASARKGFKAKTSMENGWGTNVKPIVVSNKSYIEDARKWLDTAVKPVWEN"
BASE COUNT          641 a          815 c          720 g          706 t
ORIGIN      
        1 cgcccccggc atgttttcca cgacaaatgt cggattgccg gaaatatgct tccctatgtg
       61 acgggcgagc atgcgggaat aggcgtcgaa tccgcctccc ggcgtagtcg ccacgatcaa
      121 gcgaatcgtc ttacccttgt aaaaatcctg tgccgccacc acgccggccc aacaaatcac
      181 tgccaacaat accagcgctg agagcatacc tggaaaaaac ttcttcctca tgttgtcacc
      241 tcattttttc ccaataccgc acgataacca tcggtgatgc cggggaacat agtacctatt
      301 ttgcgttgac tcaagctttt ttagctcgat cttttggctt tgcctgatca agtctcggag
      361 atctccgatg atcacgaact atttcgtcgc gatgcagtgc tttgagaaat gttctaacgc
      421 tttacctgat ggggcggcgg tggtagcgta aagaagaggg taatgagtcc aaagggtcgt
      481 gtaaatcact gctaacccac cagcggatat cgggaggcag atatggaacg agccaaatat
      541 aatgaatatt atgcctcgtt tttagcggtc gttctcttgt tttcacccca actagccttc
      601 gcacgcggca tgagtggcgg gccagccggt agggctggga gttcttcgtt cagctccgta
      661 gggtctagat cggcccgcat ggtcgttagt ggtccacgcg ttctcgtccc aagtccggga
      721 tccggcgctt tcatggctgc tggaaatcgt ctagacccca gaagaagtat tcagcccttc
      781 attacggcag gtgcaaggac aagcgcacag tcgtctttct tccctatctc gcctaacgga
      841 caccgtgtgg attttcgcca tcgccctcgg atcatcgtaa ataggttcag cgctcccgtt
      901 attgttgtcc cattccagcc ttttggctac tatccgtact gcgatagctt ttactttggc
      961 gcttacggtt gtccatcccc ttacacccca ttcgcccctg tttatgtgcc tgtacctgtg
     1021 tcctcttact cgggcgaaaa tccttatgcc gatgataact ctgtaagctt cccggcagac
     1081 atcgctgact acactccgtc aagcggcaca accgacccag cggcagtgag cccagaatcg
     1141 ttcgagcgac agggcgagta tgcattccgc gcgcgtgatt acatggcggc gaatcgtgat
     1201 tggcaacacg cggtcgtgga tgatcccagc agcggcgcgt tagctatgaa gctggcttta
     1261 gccatgttcg cggtgggcaa gtaccgggaa gcagccggta caactcagca ggtattgatg
     1321 ctcctccccc aggagaagtg gggacaggcg gtcagtgatt acaagaaact atatgcaaac
     1381 cctcaagatt acttggacca actgaaaaac ctggccaagg ctgccgcaga taaaccaaac
     1441 gatcccgcac ttcgtttctt gctagggttt cattatggtt actcaggccg ggttgctgat
     1501 gccgtgcgtg agctcaataa gcttgttcag ttggaaccga aagatcagct cggccgcaag
     1561 ctgcgagacc tgatggccga caaaacgtat aaagacaata actctgccat tgccacggcc
     1621 ccaagtgaca tctatgtccg taaggattcc aatggaacac tctatttctc gaacattccc
     1681 gcagatctaa actatcagcc cgcaacttgg caccgtacac agtattgaac tgaaacgtat
     1741 cggaaccacc gccatcaaag taagggaaaa cgctagctcg gccgtcgccg acgtcgagct
     1801 tttcaacatg ccggccaaat tgcgaagata ctccttcggt gcatcatttg ggggtcaggc
     1861 aaaccttatc gatctctttc gccatcgttt tttcccgaag agctagttct cccacaccgg
     1921 tttgacggcg gtgtccaacc actttcgcgc atcctcgata taggacttat tcgaaaccac
     1981 gatcggtttg acgttagtac cccagccatt ttccattgag gtcttggctt tgaagccctt
     2041 ccgagcactc gctctaccta ctgcgtcaat cgccgattgc ccttcgggcg agatcagcca
     2101 ttcggcaaag agcttggcgg ccgtcggatg aggcgcttta acatagatgg cagcctgttg
     2161 tggcgagacc acgggcccag cgcctctcgc aaaatcgatc ggactgccga tctccttttc
     2221 gcgcacgacg tcatggaaga gcgccggcat cactatggag tattcgccgg caactaccaa
     2281 tcgaaacata gcgctcgtgc ttttgtgaat agaaacgttc agagacttca gccccctgat
     2341 gaaatttgcc cagcgcgcat cgtccttcca gtcatcttta aaggccgcga gcatggaact
     2401 tagcggacct gccctcatgg gatcatccag cgctacgatc cctttatatc ttggattcgt
     2461 tacatcctcc caactcttcg gggcctcttc gcgtttcagt ttcttcgtgt tgtaagcaag
     2521 cgcttgaatc tgcaggctga ccaccggagc gatggcgtca ttcacgatca aggaattacc
     2581 ctgcacggtt tggtaaggtt ccgttgcgcc ggccgcgtgg agaaccagcg gtgcttcggt
     2641 aagaatcaca tcgacggtcg cctgattccc ctggtattcg gtcaatgtcc gcgtcgtgac
     2701 ttccgatctc gtcgcctgcc aataggaagt attgatgccg gggaagcgtt tgctaaaagc
     2761 gcttgatagc gagcgaatat gatctacatc ccaaacagtg tagacgacca gcgccccttc
     2821 ctgcttcgcc ttggggtaaa actctttcgc cagcttttcg aagcggggat cgctttcggg
     2881 tc
//
LOCUS       DASSWZ010000330         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1357898, whole genome shotgun sequence.
ACCESSION   DASSWZ010000330 DASSWZ010000000
VERSION     DASSWZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357898"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFN58_07910"
     CDS             <1..308
                     /locus_tag="VFN58_07910"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET8917952.1"
                     /translation="KGIRRDGRAKVSGWLGPAQGDSSSQIILYAVDLVSLQPYLVRMG
                     EAHVRQGTLDLRLNSEVQADRLDGKGKIIIRDLEFAPSRGYLGDLHGDSSQRADQLS"
     gene            271..555
                     /locus_tag="VFN58_07915"
     CDS             271..555
                     /locus_tag="VFN58_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917953.1"
                     /translation="MGIPRSALINFLKNNENAIDVDFVLVGDTSNPRFSINEAIATRV
                     AMGMAAEIGVGIRGLAEDFGTLGRRSVEGAAGVAEGVGSVVRRLFGGTRK"
     gene            762..1640
                     /locus_tag="VFN58_07920"
     CDS             762..1640
                     /locus_tag="VFN58_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917954.1"
                     /translation="MAKFRIQSHGRLQEWVAEEKGYFKDEGLDYEFLVKPIATWSSEI
                     ESTESAPEEVQRGAFESIEEGRGCNLSLACHWTVNMAASAGHGKMYTKGYAVTPSGIF
                     VAPESLIKTPGDLAQVPVTVGYHSGSHFSTLQNLEKFLNRDEIKLHFAGLLLDRLALL
                     VDRRVPAVSLFGAPSYVVEQLGFRKVVDTTFMVGHLVSGDANTEDVDRYFKALRRAQQ
                     DIDMEPELYKHYFLKELPERYHGMVDIRAFGPGERLVFEPYPREIFERTRKWIESWNL
                     FPADQAGNAGYEGSVI"
     gene            1834..>2103
                     /locus_tag="VFN58_07925"
     CDS             1834..>2103
                     /locus_tag="VFN58_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917955.1"
                     /translation="MSRVLVVEKTGHIQALLKERFSADHISVDSVPFVAGVLERIKTQ
                     GYDVLIWDAAASKTEQSKGLELLDLLTKDSNKTYIIVLADQEGGPF"
BASE COUNT          466 a          519 c          621 g          497 t
ORIGIN      
        1 tgaaggggat cagacgcgac ggccgggcaa aggtctccgg ctggctgggt cccgcccaag
       61 gagactcttc atcgcaaatc atactttatg cggtggatct tgtgagtctc caaccctatc
      121 tcgttaggat gggcgaggca cacgtgcggc aaggaacttt ggacttacgc cttaactctg
      181 aggttcaggc ggacaggttg gacgggaaag gcaagatcat catcagagat ctcgaatttg
      241 caccctcgcg cggctatctg ggagaccttc atggggattc ctcgcagcgc gctgatcaac
      301 tttcttaaaa ataatgagaa tgccatcgac gtagactttg tcctcgttgg cgacaccagc
      361 aatcccagat tctcaatcaa cgaagccatc gctacccgcg ttgcgatggg gatggcagcg
      421 gaaatcggcg tcggcatcag gggcctagct gaagatttcg gaacgctcgg cagacggagc
      481 gtagagggcg ctgctggggt agccgagggt gtcggctctg ttgtcaggcg tctctttggc
      541 ggtaccagga aataattgat ctcctgacgg tcaccggtac gagacggcgc cgctgcggga
      601 cttttgcctt ccaatacacc cggtattgaa tcagctgttg attttgactg gctattgagt
      661 ttgacttgac ggctgagtgt gctgtcgaat agattccaga aagctcagct ccaaatcagt
      721 ctgggacgac cagtgatttg gggctctgaa ggagaagcca aatggccaaa tttcggattc
      781 agtcgcacgg gcggttgcaa gaatgggtgg cggaagaaaa aggttacttc aaggatgaag
      841 ggttggacta cgagtttctt gtaaagccga tcgcgacttg gtcgagcgaa atcgagtcca
      901 cggaatcagc tcccgaggag gtgcagcgag gcgccttcga atccatcgaa gagggtcggg
      961 gatgcaatct cagcttggcg tgtcattgga ccgttaatat ggcggcctct gccggtcacg
     1021 gcaagatgta cacaaagggc tacgccgtga ctccgtcagg tatttttgtt gctccggagt
     1081 ctttgattaa gacgcctgga gatttggcgc aggtgccggt gacggtgggt tatcactccg
     1141 gcagccattt ttccactttg caaaacctgg aaaaatttct caatcgcgac gagatcaagc
     1201 tccactttgc cggtttgctt ttggatcggt tggcgctgct ggtcgatcgg agggttccgg
     1261 ctgtgagcct ctttggcgcg ccttcctacg tcgtggaaca gttgggcttc cgcaaggtgg
     1321 tagatacgac gttcatggtt ggccatctgg tttccggcga cgccaacacg gaggacgtgg
     1381 atcgttattt caaagccttg cgccgagccc agcaggatat cgatatggaa ccggagctgt
     1441 ataaacatta ttttctcaaa gagctaccgg agagatatca tgggatggtc gacatccgag
     1501 ctttcggccc cggcgagcgg ttggtatttg agccctaccc gcgagaaatt tttgaacgga
     1561 cgcggaaatg gatcgagtcg tggaatttgt ttcccgctga tcaggcgggc aacgccggct
     1621 acgaaggatc agtgatttag ttcccgctac aagacgctct gtcaccgcag gcgttgcata
     1681 atttgctgca acagagcgcc ttttttgacc ctcagtatca ccgcgcgctt ctgctgctcc
     1741 cctgcgcgcg ctaaccgtcg cagtatgcca gggcccgact ggactcatgg tgatctcatc
     1801 tgatagagcg taagaccaaa ggcgtaggtg cctatgtcaa gagttctggt agttgagaaa
     1861 accggtcaca ttcaagcgct gctcaaggag cggttttcag cggaccatat ctctgtggat
     1921 tcggtcccct tcgttgccgg ggtcctagaa aggattaaga cgcagggcta cgacgttctc
     1981 atctgggatg ctgccgcttc gaaaacggaa caatccaaag gccttgagct gcttgatctg
     2041 ctgacaaagg attcaaacaa aacctacatt attgttttag cggaccagga aggcggaccg
     2101 ttt
//
LOCUS       DASSWZ010000331         6054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1300932, whole genome shotgun sequence.
ACCESSION   DASSWZ010000331 DASSWZ010000000
VERSION     DASSWZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300932"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFN58_07930"
     CDS             <1..1108
                     /locus_tag="VFN58_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET8917956.1"
                     /translation="GPANPMEDVPGPKSKCPPYEFYRFQRFTPKSRWLMAQRVFHGLD
                     PITPELKEVIYTCTNCLMCQELCGVRDDGYGPWDITVAMREEMTERDGPIEAHRPLFE
                     GLQRYDHPWSQPKAERGLWANGLGLKKFGSGSATTLLFAGCSADRDSGRAGAIALAKL
                     MQKAGEDFVILGDEEKCCGLYAYDLGFRREYDRLENENLNRIKEAGIRKVVVACGSCQ
                     RIWRQYAKKADPQIAVLHGVEYVEDLVQTGRLKFSKTVLKKVTYHDSCHLGRGCEVYQ
                     APRTVLRSIPGIELVEMERNRRWAWCCGGGGGVPEAYPELAQWNAADRMREATETGAE
                     LVLTSSALCQRSFSTCSEGALPTMDLLEFACQAL"
     gene            1118..1852
                     /locus_tag="VFN58_07935"
     CDS             1118..1852
                     /locus_tag="VFN58_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917957.1"
                     /translation="MVKEPHFTPQKMLGMLGLKADSLGPYALIPGPKERSQMLLGLLD
                     NPAKNFTFLDYEMHTGTIDGKRVTVGNGGRFAPDTAMTTEILCAAGVQSLIRIGSCGS
                     LQEHVKIGDLVIVTGAIRGEGTTSYYVPKNFSTIANPDIVEALKAAAESLDVRHHLGW
                     IFTTDALFQETPELIQELNEQKVTSIDMVTSTFLTIAQLRKKKAGAILAVSDECLYGK
                     FAFRDPVFLAAEEKMVEVAQKALDYL"
     gene            1881..3419
                     /locus_tag="VFN58_07940"
     CDS             1881..3419
                     /locus_tag="VFN58_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917958.1"
                     /translation="MKLVHSPLFEPVLWEGRGFKILDETLIPERIEYIWIDQVAQAID
                     AVREMKTRAFGQVLTFLYGGALLAREYQGDDPAPLRNQLTQMTQQFCAARPTFDFNGL
                     GDFFSRWIAGLPAGAQAGERIAEQAREFAEQIIKARLARAERTAALLPDSARLLTHCN
                     ISGELVAIAQLCKSMGKEISVIATETRPYLQGSRLTAWELAQASVSVSLVPDCAAAQV
                     MDRGEVNAVVVGSDRCAQNGDVINKVGTYPLALMAKQYGVPFHVLVQDPGGLVRGEDV
                     TIEERSAAELLEFHGRPLVAEGAANLACRYPAFDMTPASLISSLVGFDDLFTPESFRK
                     KYLKMPSTAAKNGSQSQAQYLLVYGVPSKENYSFLAHALKAEQAHSVLVPEMRPQLWG
                     ARVVARELLSRSTPTTLISDNMMGTLFAQGEIRKLCLFYDGLSERGPRGICGSLLAVR
                     LACHHKVPIELLASETPEGGGADRDVSTFLGQRICPAGVSVHPIESEILPWAIFKNAS
                     GASS"
     gene            3416..4420
                     /locus_tag="VFN58_07945"
     CDS             3416..4420
                     /locus_tag="VFN58_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET8917959.1"
                     /translation="MTKTKREMIQWGKLLFDRRLISGWGGNLSCRVGKEGFLITGQHA
                     PLGFLVPEDLVQIDHDGKPSKPTQRASSETPLHLAVYRGTDAQAIVHVHPPMVLAFSL
                     THESFVPISFEEKYTIGEVPIVPQDTPTVTKPEQVVEELKYHPVVIIKGHGTVAIGKN
                     LQEAFLVTDLLEEAVHCQFFKESAAATRGANSHPSQVQNGKTVVLADHKSYVLFSQEH
                     MAALVERANHDSEFRRHGSETSLSTSLTLHLEETDASWTVKFVAGEITELNQKTNGEF
                     MISGKEEWWRAVFSNRIDPFLATQQGKLNLKRGELAQLSRWYKPFQRVFSLWQTIQAQ
                     "
     gene            4396..5925
                     /locus_tag="VFN58_07950"
     CDS             4396..5925
                     /locus_tag="VFN58_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8917960.1"
                     /translation="MANDSGSIGFFAAEVLEKTALRQPQKMAIIAKDEELTFTALNQR
                     VHGLAANLQQIGICQGDRVGVLLPNCAAIPLSYFATQKIGAVTVILDARLKGKELQGV
                     LKDAAVKLLIVHQQLRAEAEEAFKELGLIPLWVVNGEGDESFENKLSFPGAPASLPQL
                     RADDDALILYTSGTTGEPKGVVLSYRNLAQYPRVMGGMGITDTSTIRGCILPMSHIVG
                     PVVCNEVADKGFTLVIFDQINPVTLLEGIQQHRVTVFESVPIVFQLLLGVRDLANYDT
                     SSVKIAAMMGTSIPLPLLRAFQSAQPHIKVIQGFGLTETAPMITLVEPERAETKMGSI
                     GRAVPGVEVKIVDEAGKEVPIGEPGEIITRGPHVMKGYFRKPEATAARIRDGWLYTGD
                     VGKVEADGYYYHLGRRDDMIITGGLNVYPAEVENLIYTYPGVQETIVFAIPDPKRGYV
                     IGAALVPRPGANIVERELVTFLRGNLANFKVPQKIVVRDSLPRTSSGKLIREADTLLS
                     N"
BASE COUNT         1375 a         1591 c         1777 g         1311 t
ORIGIN      
        1 tggtcccgcc aatccaatgg aggatgtgcc cgggccgaag agcaagtgcc cgccctacga
       61 gttttatcgc ttccaaagat tcacgccgaa gtcgcgctgg ctcatggcac agcgtgtgtt
      121 tcacgggctc gatcccatca cccctgagct gaaagaagtc atctacacct gcacgaattg
      181 tctgatgtgt caggagctgt gtggcgtccg tgacgacggc tacggaccct gggacatcac
      241 agtcgcaatg cgcgaagaga tgactgaaag ggatggccct atcgaagccc atcgcccgct
      301 cttcgagggt ctgcagcggt atgaccatcc atggtcgcag cccaaagctg agcgagggct
      361 gtgggcgaac ggactgggat tgaaaaagtt cggcagcggc agtgccacca cgctgctatt
      421 cgccggctgt tcagccgatc gggacagcgg gagagccggc gccatcgcgc tcgcgaagct
      481 catgcaaaag gccggtgaag atttcgttat tctaggggac gaggaaaaat gctgcgggct
      541 ttatgcttat gacctaggtt tccggcggga gtatgatcgc ttggaaaacg agaacctcaa
      601 caggattaag gaagctggga tccgcaaagt tgtggttgcc tgcgggagtt gccagcgcat
      661 ctggcggcag tacgccaaaa aagccgatcc ccaaatcgct gttcttcatg gagtcgaata
      721 cgtcgaagat ctggtgcaaa cgggccggct taaattcagc aaaaccgtgc tgaaaaaagt
      781 cacctatcac gactcttgcc atcttggccg gggctgcgaa gtttaccaag cgcctaggac
      841 cgttctacgt tctattcccg gaatcgagtt ggtggagatg gagcgcaatc ggcggtgggc
      901 ttggtgttgt ggcggtggcg gtggtgtacc ggaggcctat cccgagttgg cgcaatggaa
      961 tgccgcggat cggatgcgag aagcaaccga aaccggcgcc gaattagtgt taacgtcaag
     1021 cgcgctgtgc cagcgtagct tctctacctg ttctgagggt gccttaccaa cgatggactt
     1081 gctggagttt gcttgtcagg cgctttgaat caattcaatg gttaaagaac cgcacttcac
     1141 tccgcagaag atgcttggaa tgttgggcct gaaagccgat tccctcggac cctatgcgct
     1201 gattcctgga ccgaaggagc gcagtcaaat gttgcttggc ctgctggaca acccagccaa
     1261 gaattttacc ttcttggatt acgagatgca caccggcacc atcgacggaa aacgagtgac
     1321 cgtcggcaac ggcgggcgct tcgcaccgga cactgcgatg actacggaaa ttctctgcgc
     1381 cgcgggcgtg caatcgttga ttcgcatcgg tagctgcggt tcgttgcaag agcacgtgaa
     1441 gatcggtgat cttgtcattg tcactggcgc gatccgcggc gagggaacga cgagttacta
     1501 cgtgcccaag aatttctcca cgattgctaa ccctgacatc gttgaggctc tgaaagcagc
     1561 ggcggaaagt ttagacgttc gccatcacct gggctggatt tttaccaccg atgcgctatt
     1621 tcaggaaacg ccggaactga ttcaagagct gaatgaacag aaggttacca gtatcgatat
     1681 ggtgacgtcg accttcctca ccatcgcgca gctccgcaaa aagaaggctg gcgccatttt
     1741 ggcggtttcg gacgagtgtc tctatggtaa gtttgctttt cgcgacccgg tatttctcgc
     1801 cgctgaagaa aaaatggtcg aggtagcgca gaaagcatta gactatctgt gaatcttttg
     1861 tttaccctca gtgtagaaac atgaaactcg tgcacagtcc tctgttcgaa ccggtgttat
     1921 gggaagggcg cggcttcaag attcttgacg aaactttgat cccggagcgg atcgaataca
     1981 tttggattga tcaggtcgca caagccatcg acgccgtcag ggagatgaaa acgcgagcct
     2041 tcggccaagt gctgactttt ctgtacggcg gagcgttgct cgcccgggaa tatcaaggtg
     2101 atgaccctgc ccctctccgg aaccagctaa cgcaaatgac tcagcagttc tgcgcggcac
     2161 gcccgacatt cgatttcaat ggcctggggg atttcttctc caggtggatt gccggcttgc
     2221 cggccggcgc tcaggctggt gagcggatcg cagagcaggc gcgtgaattc gcagaacaga
     2281 ttatcaaagc gcgcctggca cgagccgaac gaactgccgc ccttctgccg gattccgcgc
     2341 gcctattaac ccactgtaac atcagcggcg aactggtggc gattgcgcag ctctgtaaat
     2401 cgatgggcaa ggaaatctcc gtgattgcca cagagacgcg gccgtatctg cagggatcgc
     2461 ggctcacggc gtgggaactg gcacaggcaa gcgtttcggt atccttggtt ccagactgcg
     2521 cggccgccca ggtgatggac agaggagaag tcaacgcagt cgtcgtgggt tccgaccgct
     2581 gcgcgcagaa tggcgacgtg atcaacaagg taggaactta tcctttggcc ttgatggcga
     2641 aacaatacgg cgttccattc catgtgttgg tgcaggaccc cggtggtttg gtcagaggcg
     2701 aggatgtgac gattgaagag cggtcggctg ctgagcttct cgagtttcac ggccggcctc
     2761 tggttgctga aggcgcggcg aatctggcct gtcgttatcc ggctttcgat atgactcctg
     2821 cttccttgat cagctccttg gttggttttg atgacctgtt cacgccggaa agctttcgga
     2881 agaagtattt gaaaatgcca tcgacagccg caaaaaacgg cagccaatcg caggcgcagt
     2941 atctgttggt ttacggcgtg ccgagtaaag agaactattc cttcttggcc catgcgctta
     3001 aggccgagca ggcgcacagt gttttggttc cagagatgcg accgcagctg tggggcgctc
     3061 gagtcgtggc ccgcgaacta ctttctcgca gcacaccgac aactttgatc tctgacaaca
     3121 tgatggggac cctgttcgcg caaggcgaga tccgcaaact ctgcctattt tatgatggat
     3181 tgagcgagcg agggccgcgc gggatttgtg gctcgctgct ggcggttcgc ttggcgtgcc
     3241 accataaggt accgatcgag ttactcgcgt cggaaacgcc agagggtgga ggagcggacc
     3301 gcgacgtgtc gacttttttg ggtcagagga tttgtccggc aggggtctcc gtgcatccca
     3361 tcgagagcga aattctgcct tgggcgatat tcaaaaacgc aagcggtgct tcttcatgac
     3421 caagaccaaa cgagaaatga tccagtgggg caagttgctt ttcgaccggc gccttatcag
     3481 cggttggggt ggaaacttaa gctgccgagt cggcaaagag ggattcttaa tcaccgggca
     3541 acacgcgccg ctcggttttc tcgtgcctga ggatctcgtg cagattgatc atgatgggaa
     3601 gccgagcaaa ccgacgcaac gcgcctcgag cgaaacgccg ctgcatctcg ccgtgtatcg
     3661 gggcaccgat gcccaagcca tcgttcatgt tcatccacca atggtgctcg cgttttccct
     3721 gacacatgag agtttcgttc ccatcagttt cgaggagaaa tacaccatcg gcgaggttcc
     3781 cattgttcca caggatacgc cgacggtgac aaaaccggag caggtcgtcg aagagctcaa
     3841 gtatcatccg gtggtgatca tcaagggtca cggcacggtt gccatcggca agaatcttca
     3901 agaagccttt ctagtgacag atttgctgga ggaagcagtg cattgtcagt ttttcaaaga
     3961 aagcgctgcc gcgacgcgcg gggcaaactc gcacccgagt caagtgcaaa atgggaaaac
     4021 cgtggtgctt gctgaccaca agagttacgt cttgttttcc caggaacaca tggcggcgct
     4081 cgtggaacgc gccaatcacg attctgaatt tcgccgacac gggagcgaga cgagtctttc
     4141 tacgtctctc acccttcatc tagaggaaac cgacgcctct tggaccgtga aattcgtcgc
     4201 cggtgaaatc accgagctaa atcagaagac caatggggag ttcatgatca gcggcaagga
     4261 ggagtggtgg agagcggttt tcagtaaccg gatcgatcct tttctcgcta cccagcaagg
     4321 aaagttgaat ctaaaacgcg gtgagctggc gcaactgtcg cgctggtata aaccttttca
     4381 aagggtattt tcactatggc aaacgattca ggctcaatag gctttttcgc ggcggaagtg
     4441 ttggaaaaga cagcactccg gcaaccacag aagatggcga tcattgccaa ggacgaggaa
     4501 ttgaccttca cggcactgaa ccagcgggtt catgggctgg cggcaaatct gcaacagata
     4561 ggaatttgcc aaggcgatcg cgtcggagtt ctcttgccca attgcgctgc gatcccgctc
     4621 agctacttcg ccacgcaaaa gatcggcgcg gtgaccgtta ttctcgacgc gcgattgaag
     4681 ggaaaagagc tgcaaggcgt tctgaaagat gcggcggtca agttgcttat cgtgcaccag
     4741 cagctgcgcg ccgaggcgga ggaagctttc aaagaactag ggttgattcc cctctgggtg
     4801 gtcaacgggg agggcgatga gagtttcgag aataaacttt ctttcccggg agcaccggcc
     4861 agtttgcccc aactgcgcgc cgatgacgac gcgctgattc tttacacttc cggaactacc
     4921 ggggagccca agggcgtcgt gctcagctac cgaaacctcg cgcagtatcc ccgcgtaatg
     4981 ggcgggatgg gcatcacgga tacctctacg atcagaggct gtatcctgcc catgtcccat
     5041 atcgttggac ccgttgtttg caacgaggtg gcggacaaag ggtttacttt agtgattttt
     5101 gatcagatca atccagtcac gcttttagaa ggcatccagc aacaccgggt tacggttttc
     5161 gaaagcgtgc cgattgtctt tcaacttcta ctaggagtgc gcgatctcgc caactacgat
     5221 accagcagcg tcaagattgc cgcgatgatg ggaaccagta ttccgctgcc gctgctacgc
     5281 gcgttccaat cggctcaacc tcacatcaaa gtcatacaag gcttcggact gaccgaaact
     5341 gcgcccatga tcaccttggt ggagccggag cgagcagaaa ccaagatggg cagtattggc
     5401 cgggccgtcc ctggcgtcga agtcaagatc gtcgatgaag cgggcaaaga agtgcccatc
     5461 ggggagccgg gtgaaattat cacccgcggc ccgcatgtga tgaaaggcta ttttcgaaaa
     5521 ccggaagcaa cggcggcgcg cattcgcgat ggttggctat acaccggcga cgtgggcaaa
     5581 gtggaagcgg atggctatta ctatcatttg ggacggcgtg acgacatgat catcaccggc
     5641 ggactaaacg tttatcccgc cgaggtggag aacctgatct atacctatcc gggggtacag
     5701 gagacgattg tctttgccat tcccgatcca aagcgcggat atgtgatcgg cgcggccttg
     5761 gtgccacgac cgggagcgaa cattgtcgaa cgagaactgg tgacctttct gcgcggcaat
     5821 ctggcgaact tcaaagtccc gcaaaagatc gttgtccgcg actcgctgcc gcgtacttcg
     5881 tcgggaaaac tcatccggga ggccgatacc ctcctctcga actagaggtc gattgcatgg
     5941 ccgacgctct tcccaacggc gacgaaatta ttgactcgat caaaaacgcc gccggtatca
     6001 tactcggggt ttccgaaaac cgcagttgga tcgagctttt cttcgaaggc gacc
//
LOCUS       DASSWZ010000332         6562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_933167, whole genome shotgun sequence.
ACCESSION   DASSWZ010000332 DASSWZ010000000
VERSION     DASSWZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933167"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFN58_07955"
     CDS             complement(<1..858)
                     /locus_tag="VFN58_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET8917961.1"
                     /translation="MIEGEPEKVNGFGRSNSAGLMPPPSVIWALAIAFVTLIVYLSRG
                     FVLLLFLSATVAYLLNPIVKVAEGAMIKRRVAVAVIYLGIGVAFLVTAYFVFPRLRAE
                     VNTLSGNLPFFGQRFDEAVDVLQNEIVERYPAADGLFTPRELRYEKLNDFIEQQVADL
                     PLLLGHLASIVLAGVLIPFFSFFFLRDSRKIVKFVLDRLPANHIETTVAVWCEIDRTV
                     GRYLRGLAIEGILVGVSAALGLAILGVNYPLLLGLVTGLANIVPYLGPIIGGATAVLV
                     ALVQYKSLTV"
     gene            complement(851..1423)
                     /locus_tag="VFN58_07960"
     CDS             complement(851..1423)
                     /locus_tag="VFN58_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET8917962.1"
                     /translation="MKVHSMAKWRWLEHVTDREQLLRLKVLANVPLFAGLSRRQLGKL
                     LVKFFEKEYPSGETIFSEGEPGKALFIVLTGRVSICRSLKKEEELLATLTAGGYFGEL
                     ALIDDQPRFATVRALEPSVLLILYKSDFDDLIEGHRTLAIRVMGNLLKTLATYVRRAQ
                     SQANGRNSMSPGRTSAPDQSTVTSEDVPRD"
     gene            1660..2166
                     /locus_tag="VFN58_07965"
     CDS             1660..2166
                     /locus_tag="VFN58_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HET8917963.1"
                     /translation="MSVEEHKAKGKRSHNCFVVTVSDTRDETTDESGQLIKSMLTDET
                     HLLAGYRIVKDDPTEIVARLREALANEQVEAVIVNGGTGISPRDGTYEVIVGLLEKRL
                     DGFGEIFRYLSYQDIGSAAIMSRAVAGSAQGKVLISLPGSKGAVKLAMEKLILPELRH
                     MVSQLQGK"
     gene            2163..2408
                     /gene="moaD"
                     /locus_tag="VFN58_07970"
     CDS             2163..2408
                     /gene="moaD"
                     /locus_tag="VFN58_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HET8917964.1"
                     /translation="MKVKVKLFAILRERAGTAEVTKEIQEGSTVADLWAALQQDYPKL
                     AVPGIRLLYAVNQDYVRPDRVLKNSDEVVFVPPVSGG"
     gene            2411..2842
                     /locus_tag="VFN58_07975"
     CDS             2411..2842
                     /locus_tag="VFN58_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET8917965.1"
                     /translation="MFRVTDKPIDLHELVDCVTDPEAGAIATFIGTTRNNNQGRKVIA
                     LDYEAYPEMAEKELARLGEEAKKKWQICRMAIVHRIGPVQITEPSVIIAVSAAHREAA
                     FAACRFAIEEIKKTVPIWKKEVYEGGEIWIGTQSGQPLNRS"
     gene            complement(2883..3533)
                     /locus_tag="VFN58_07980"
     CDS             complement(2883..3533)
                     /locus_tag="VFN58_07980"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET8917966.1"
                     /translation="MTEILNWIIDSIRAHGPWSVFVGVIIESVIVPIPSPLIIMGAGF
                     LLISAELSFLQAFAPIITQIVLPGSIASTLGAYIGYAIGYVGGKPMVERLQGFLGFNW
                     SDVEAMERRFRVGQVNTSLFFLRALPIFPLSVISAGAGLLRLPIKQFTLWTFYGSIAR
                     CLFLGYLGWGLGEPYQAMAKGIDKIEAMVSLLLLGLIFGVILWLRWRVRGRIMEND"
     gene            complement(3619..5058)
                     /locus_tag="VFN58_07985"
     CDS             complement(3619..5058)
                     /locus_tag="VFN58_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET8917967.1"
                     /translation="MSDNQAWGTLPPFKKAPLPPLPSMWSALGPGVIFMALAQGSGEL
                     IWWPYIIAKYGLSFLFLLLPACLLQFPVTYEIGRYTVLTGESIFQGFIRLNRGFAFFL
                     WILMTLSFLWFGAFAAAGGTSLAALTNFPAGWSPRGQTLFWGYLSMFVFLTAILLSKV
                     IYRLVESFMWAVALLTLVGLLWASANAEALHALPAFVKGLFIPASPMPRAWDPADATK
                     LLTAITFAGLGGFWTLFYSYWIRDKGAGMAYYMGRLTGPITGKAEAIPASGFVPGEDE
                     GLAHVARWRRYLFWDVSIGIGGNLLTTLMTCLLAYALLFPKGLLPQGYELAVVQSRFF
                     EVSWGWLGRILFLIVAAAFLSDTWLATVDAVSRIHTDCLYGFFPSTQSVSVRSWYLIF
                     LTMLTAITAATMGFAEPGPLILLSAVIGFVGTVLFSVALIFLNHVYLPRQLPAAARPG
                     RVNLIFLCIACAAYSLLAVAYLLTIAKII"
     gene            complement(5069..5797)
                     /locus_tag="VFN58_07990"
     CDS             complement(5069..5797)
                     /locus_tag="VFN58_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET8917968.1"
                     /translation="MSKTAGIVIIGNEVLSGKTQDINSHFFCAELRQLGVDVQKISTI
                     PDEIELIGQEVAAFSKRFNWVFTSGGVGPTHDDVTIDGIARGFDLKVIRHPDIERRMR
                     QRLGTEVNEARLRMANVPEGAELLATEALFAPIIKIHNVYIFPGIPKILQERFHAIKE
                     RFRDAPYFLKIVYVRQGEGVIAGFMNDLLIKYPQLMLGSYPVLDIPDYKVKVTLESKD
                     SDYLDQALQSFLSALPDGAVHRIQ"
BASE COUNT         1719 a         1684 c         1809 g         1350 t
ORIGIN      
        1 gactgtaagg ctcttgtatt ggaccagagc aactaaaaca gcggtcgcgc cgccgatgat
       61 cgggccaaga tagggcacta tgttagctag acctgtcacg agaccgagta agagcgggta
      121 gttgacacca aggatcgcaa ggcccaacgc cgcagagacg ccgaccaata tgccttctat
      181 ggccaagccg cggaggtacc gacctacggt tcgatcgatc tcacaccaaa ccgcaactgt
      241 cgtctcaata tggttagccg gcaaccgatc caacacgaac tttacgatct tgcggctgtc
      301 gcgcaggaag aaaaacgaga agaatggaat caacacgcct gcgagcacta tcgatgccag
      361 atggccgaga aggagaggaa gatccgccac ctgctgctcg ataaagtcgt tcaacttctc
      421 gtagcgaagc tcgcgaggcg taaacaaccc atccgcggcg gggtagcgtt cgacaatctc
      481 attttgcagg acatcgacag cttcgtcgaa gcgttggccg aagaaaggta agttgccaga
      541 aagagtgttc acttcggcgc gcaaacgcgg aaatacaaag taggccgtga ccaggaaggc
      601 aacgccaatc cccaggtaga tcacggcgac ggctactctg cgcttgatca tggcaccttc
      661 ggcgactttt actatcgggt tgagcaagta agcaacggta gccgacagaa agaggagcag
      721 gacgaagccc cgcgagagat acacgatcag agtcacaaag gctatggcga gggcccagat
      781 gactgaaggc ggcggcatca atccagcaga gtttgagcga ccaaatccat tgactttttc
      841 cggctctcct tcaatcacgg ggaacgtctt ccgaagtcac cgtactctgg tcaggcgcgg
      901 atgtgcggcc agggctcatg ctgttacggc cgttggcttg agactgagcc cggcgaacgt
      961 acgttgccag agttttcaac agattaccca tcactcgaat ggccagcgtt ctatggccct
     1021 ctattagatc gtcaaagtcg ctcttgtaca ggatcagaag aaccgatggt tccagcgcac
     1081 ggacagtggc aaagcgcggc tgatcgtcga taagcgccag ctcaccgaaa tagccgccgg
     1141 ccgtgagtgt tgcaagtagt tcttcttctt ttttgagtga gcggcagatc gatactctgc
     1201 ctgtgagaac aataaacaac gctttaccgg gctccccttc gctgaagatt gtctcgcccg
     1261 atggatactc cttttcgaag aactttacca aaagtttgcc gagttgcctt cggctcaaac
     1321 ctgcaaagag cgggacattc gcgagaacct taaggcgcag tagctgttca cgatctgtta
     1381 cgtgctcaag ccagcgccac ttagccatag aatgaacttt catcggtgat gtccacagta
     1441 acacaaaacc atggaaacgg ctttaccttt atgcttccgg catcgcttgt caagggttct
     1501 cagactggtg gcttatcacc ttgaagtcgg agcacgttga atcttgcccg gtcttagtga
     1561 tattcgccta tcaggctcaa ggccgaattt catgactttt gaaatcattt aacatggttg
     1621 ttatagttca ctaatggaat cggtaaaaag gcaaccacta tgtccgtaga agagcataag
     1681 gcaaagggca agcgaagcca taactgtttt gttgtcaccg tgagtgacac ccgcgatgag
     1741 actacagatg agagcgggca attgatcaag tcaatgctca ccgacgagac gcacctgctg
     1801 gccggttacc ggatcgtcaa ggatgacccg accgagattg tagcacggct gcgcgaagcg
     1861 ctcgccaacg agcaggttga ggcggttatc gtcaacggcg ggaccggcat ttcgccccgc
     1921 gatgggacct acgaggttat tgtggggtta ctcgagaaaa ggcttgacgg gttcggggag
     1981 atctttcgct acttgagcta tcaagacatc ggctccgctg cgataatgag tcgggcggtt
     2041 gcgggatcgg cgcaaggaaa agttcttatc tcgcttcccg gttccaaagg cgccgtaaag
     2101 ttggccatgg agaaactcat cttgccggag ctgcgccata tggtctctca gctgcaagga
     2161 aaatgaaggt caaagtcaaa ctcttcgcga ttctaagaga gcgtgctgga accgccgaag
     2221 tgacaaaaga aatccaagaa ggcagcacgg tagcggatct ctgggcggcg ctgcaacaag
     2281 attatccgaa gttggctgtt cctgggatcc ggctacttta cgctgtcaat caggattatg
     2341 tgaggcccga tcgcgtcctc aagaattccg atgaagtcgt cttcgttcct ccggtgagtg
     2401 gagggtaaag atgttccgcg tcaccgataa gcccatcgat cttcatgaat tggttgattg
     2461 cgtgactgac cccgaggcgg gagccatcgc gactttcatc ggcaccacgc gcaacaacaa
     2521 ccaagggcgc aaagtcattg ctctcgacta cgaagcctat ccggagatgg ccgaaaagga
     2581 actagcccgc ctaggcgagg aagccaaaaa gaagtggcag atctgccgca tggccatcgt
     2641 ccatcgaata ggtccggtgc agattaccga gcccagcgtg atcatcgctg tttcagcggc
     2701 tcatcgcgaa gctgcgtttg ccgcctgcag attcgccatc gaggaaatca agaagacagt
     2761 gccgatctgg aaaaaagaag tttatgaagg cggcgaaatc tggattggca cccagagcgg
     2821 ccaaccgctc aatcgttctt agagagtata cacgatggga gggttgggtg gttaggctga
     2881 tgtcaatcgt tctccatgat tcttcctctt acccgccagc ggagccacag gatcacaccg
     2941 aaaatcaggc ccaatagtaa caaagagacc atggcctcaa tcttatcgat gcccttcgcc
     3001 attgcttggt aaggttcgcc tagcccccag cctaaatatc ccagaaaaag gcagcgcgcg
     3061 atggagccat aaaaggtcca cagcgtgaac tgtttgatgg gaagccggag cagtcccgcg
     3121 ccggcggaga ttactgagag tggaaatatt ggcaatgcgc gaaggaaaaa aagcgaagtg
     3181 ttgacttgac ctacacgaaa acgacgctcc atcgcttcga cgtccgacca attgaagccc
     3241 agaaaacctt gcaaacgctc aaccataggc ttgccgccga catagccaat ggcgtagcca
     3301 atgtaagcac ccagggtgga agcgatggag cccgggagaa caatttgggt tatgatcggg
     3361 gcgaaggctt gcaaaaatga gagttcggcg ctgatgagca aaaagccggc tcccattata
     3421 atcaaaggcg agggtatcgg gacaatgaca ctctcaatga taacaccaac aaaaacactc
     3481 cagggaccgt gggcacggat agagtctatg atccaattga gaatttcagt caatgcagtt
     3541 ctcctccggc taaaagttct gctgaagata gcagttcttg ttttcgaact taaacgaatt
     3601 gtttgcgccc attaatcatc aaatgatctt agcaatagtt aggaggtagg caacggcgag
     3661 cagactgtag gcggcgcagg cgatacacaa aaagatcaag ttaacgcgac ccggacgagc
     3721 ggcggcgggt aactgacgcg gcaaatagac atgatttaga aaaatcaacg ccaccgagaa
     3781 aagcacggtg ccgacgaacc cgataaccgc tgagagcagt atgagcggtc cgggctcggc
     3841 aaaacccatc gtggcggcgg tgatggcggt gagcatggtc aggaaaatca gataccagga
     3901 gcgaaccgac acggattgtg tgcttgggaa aaacccatag agacaatcgg tgtgaatgcg
     3961 gctcaccgca tcgacggtgg cgagccaagt gtcggataga aatgccgcgg cgacgatcaa
     4021 aaagagaatt cgtcccaacc aaccccagct tacctcgaaa aaacggcttt ggacgacggc
     4081 aagttcatag ccttgcggca aaagcccttt agggaacaag agcgcatagg ccaacaggca
     4141 ggtcatgaga gtcgtgagga ggtttccgcc gatgccgatg ctgacatccc aaaacagata
     4201 gcgcctccag cgggcgacat gggccaatcc ttcgtcttcg cccggcacga agccggacgc
     4261 aggaatggcc tcggctttgc ccgtgatcgg acccgtgagt cgccccatgt agtacgccat
     4321 ccccgcgccc ttgtcgcgaa tccagtagga ataaaagagc gtccaaaatc cgcccagtcc
     4381 ggcgaaggtg atcgcggtca gcaattttgt tgcatcagcg gggtcccagg ctctcggcat
     4441 cggcgacgca ggaatgaaca aacctttaac gaaagcgggt agagcgtgta gcgcttccgc
     4501 attggcactc gcccacaaga gtcccacgag agtcagaagc gccacagccc acataaagct
     4561 ttcaaccagg cgatagatca ctttgctcag aagaatcgca gtaagaaaca caaacatcga
     4621 gaggtacccc caaaaaagag tctgacctct ggggctccaa cccgctggaa aattcgtcag
     4681 ggcggctaac gatgttccgc cggcggcggc aaacgcaccg aaccaaagaa atgaaagcgt
     4741 catcaagatc cagaggaaaa atgcgaagcc gcgattcaag cgaataaagc cttgaaagat
     4801 gctttccccg gtgagcacgg tgtagcgtcc gatttcatag gtgacgggaa actggagaag
     4861 gcaagcgggt agaagcagaa ataggaatga caagccatac ttggcgatga tgtagggcca
     4921 ccagatcagc tcgccgcttc cctgagcgag agccatgaag atgacccctg gcccgagcgc
     4981 gctccacatg ctgggaagcg gtggcagagg agctttctta aagggcggga gagttcccca
     5041 ggcctggttg tcggacattc aatgactgct actggatgcg gtgaactgcg ccgtcgggaa
     5101 gcgctgaaag aaaactttgc agcgcctgat caaggtaatc ggaatctttg gattcgaggg
     5161 tgactttgac cttataatcg ggaatgtcga gcacgggata ggaaccgagc atgagctgtg
     5221 gatacttgat cagaagatcg ttcataaaac cagcaatgac tccttcacct tgcctgacat
     5281 atacgatctt aaggaagtag ggcgcatcgc gaaacctttc cttgatggcg tgaaaacgtt
     5341 cctggagtat ttttggtatg ccgggaaaga tgtagacatt gtggatcttt attatcggag
     5401 cgaaaagggc ctcggtcgcc aaaagctccg cgccttcggg gacattggcc attctcagtc
     5461 gagcttcatt aacctccgtg ccaaggcgtt ggcgcatccg tcgttcgatg tcgggatggc
     5521 ggataacctt gagatcaaag ccgcgcgcga ttccgtctat ggttacgtca tcatgggttg
     5581 gtccgacacc tccggaggtg aagacccaat taaagcgctt cgagaaagca gcgacctctt
     5641 ggccgatcaa ttcgatttca tcgggaatcg ttgaaatctt ctgaacgtcc acgccgagct
     5701 gacgcaactc ggcgcagaag aagtgggaat tgatgtcctg ggtttttccg gacagcacct
     5761 cattgccaat gataacgatg ccggcagttt tggacatgct cttcttttac cgcaatggaa
     5821 cgatgtcaag caagctcagg gctcgaaacc gttacatatc aaactcactg caactcataa
     5881 agaaatggca tcggggattg gagcagacaa agcggcattt gtgattgagg agcctggaac
     5941 cacacacggg acaaaattcc caccagtctt cctgcttttc ttcctttgag gggtgattaa
     6001 tgtcgatgac ggggctctta ttgagcatga tattttcttg taatcgaact agttcggttt
     6061 gacaagcgat atttgcggtc atgaaacttg tccgttggac ttcaacgtgt gctcataata
     6121 tacagagaca ggcaacttgg cgaatcgttg agaaaaccat aaaaggagtt gtcagccctg
     6181 tctaacatcg tggacaagcc tttgccgaaa aagagtttgg aagagctcag agaagagcgg
     6241 cgacgcgcgc gctggagcgc cttttggacg cccttcatca cgctcgcctc catcgttagt
     6301 tcggtggccc ttctcatgtt cttcgtgcgg gcatgtcagg atcgattgcc gttcccctta
     6361 caaccggggc cgccggctcc gctgaagaat cagtttacag gcatcagcct agggtcgagc
     6421 tccgccccag cagctcaaga gggaactcgc actagaccgt tccacggcac gtctccgcgc
     6481 ccgggtgggt ttccacggcc gtaaacaaag cttcaatttg attgaactcc tactttcgct
     6541 ataatcgcag tctgggtgtc tg
//
LOCUS       DASSWZ010000333         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1230546, whole genome shotgun sequence.
ACCESSION   DASSWZ010000333 DASSWZ010000000
VERSION     DASSWZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230546"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VFN58_07995"
     CDS             <1..1583
                     /locus_tag="VFN58_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET8917969.1"
                     /translation="AFSTPMLGCKYCISLLIPLVLSLSVHAQGGAEIKKEQVDQAVQE
                     LEKRAQQAIQNKVVPGLAIAVVFQDKVVYAKGFGVREVGKSEPVNPDTVFQLASVSKP
                     IGATVVAALVGDKKISWDSKISDLDPTFAMYDPWVTREITIRDLYAHRSGLPEHAGDL
                     LEDLGYDRAQVLYRLRFQKPKSSFRTSNAYTNFGLTEAAVAAAQAYGLEWEDASEQKL
                     YKPLGMNSTSSRFRDFMARPNKALGHVLVDGNWVHKFQRQPDAQSPAGGVSSSVNDMA
                     KWIRLKIANGKFGEEPIVAEEALVQMDHPHMLTGFNPLTGLPTFYGLGMNVSYDQQGR
                     ARLNHSGGFALGAGTHVNMVPSEQLGIVVLTNAYPVGLAEGLGFTFLDFALYGKLTYD
                     WLALFKKIFSDPAAIGTVVGTDYSKPPASPLAALKSDAYVGAYANDFFGDIEIVAKDG
                     GLAIVAGPNKMTFPLKHYDRDIFTYETTGENAVGTTGVYFTIGADGKAKTVLVENLNV
                     HKQGTFTRKQAPTDTAQD"
     gene            1857..2309
                     /locus_tag="VFN58_08000"
     CDS             1857..2309
                     /locus_tag="VFN58_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917970.1"
                     /translation="MKGSDRRERSFDTAESETLGMWGNSMRENREAPQTPTIDGVGRS
                     KKAKGRNVDVHVCGESDGLVVPTKRTNKADAEEWAAESAEDRRSTKGNAPQTILRRTQ
                     NRGSHRVCGAYGKRQEGIASVSQRAAGRQMNPKILEGKTCPTDSESFQ"
     gene            complement(2320..3258)
                     /locus_tag="VFN58_08005"
     CDS             complement(2320..3258)
                     /locus_tag="VFN58_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET8917971.1"
                     /translation="MSDRARSQELLSLWLQLLLVILFTAVAIVIAIYSDSETMALEAG
                     SAIVDLVVSVLAIFVARKVAEPANQRYQFGYAKYEPLMTTVEGILMTGICASAILSSA
                     QDIMHPDPFEAPYLIVIYSAASFFISVIFGLWMRSVGKRAGSPLVLANADLWILGGWL
                     ALGVCGAFVASIVLGRMGKIEAAAYIDPAVCLVLSLFFLKKPYDILRESISDLVDANP
                     YAGAVNAVEESAAAVAEQFHLKGVEWVRVRKAGRRVFVMVSFFEGPTHSLQDMDKVRE
                     AVTNEVVRLNPDVDIVVAFRLAPALDSGPSPTPGTI"
     gene            4302..4403
                     /locus_tag="VFN58_08010"
                     /pseudo
     CDS             4302..4403
                     /locus_tag="VFN58_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT         1076 a         1197 c         1367 g          918 t
ORIGIN      
        1 tcgcgttcag taccccaatg ctgggctgca agtactgcat cagtttgttg attccccttg
       61 tcctgtccct gtctgtgcac gctcaaggag gagccgagat aaagaaagaa caagtggacc
      121 aagccgtcca ggagctcgaa aaacgcgcgc aacaggcgat tcaaaacaag gtggtgccag
      181 gtctggcgat tgcagtggtg tttcaggaca aagtcgtgta cgcgaaagga tttggcgtgc
      241 gcgaggtcgg caaaagcgaa cccgtcaacc ctgatacggt tttccagctc gcgtccgttt
      301 ccaaaccgat cggtgcaacg gtcgtcgcag cgctggtggg tgataagaag atttcctggg
      361 actcgaaaat cagcgatctc gatcccacgt ttgcaatgta cgatccctgg gtcacgcgtg
      421 aaatcaccat tcgagatctg tacgcgcatc gcagcggatt gcccgaacac gcgggagatt
      481 tgctggagga tttgggatat gaccgtgccc aggtactgta tcgtctgcgt tttcagaagc
      541 ccaagagcag tttccgtacg agcaatgcct ataccaattt cggcctgacc gaagccgcgg
      601 ttgcagcggc gcaagcgtac gggctggaat gggaggatgc cagcgagcag aaactgtata
      661 agccgctcgg catgaattcc accagttcgc gctttcgaga ttttatggcg cgtcccaaca
      721 aggcgctggg gcatgtgttg gtggacggca actgggttca caagtttcaa cgccaacccg
      781 acgcgcaatc gcccgcgggc ggcgtcagtt catcggtgaa cgacatggcg aaatggatcc
      841 gcctgaaaat tgcgaacggc aaatttggtg aggaaccaat tgtcgcggag gaggcactag
      901 tccaaatgga ccatccgcac atgctcacag gattcaatcc gctgaccgga ctaccaacct
      961 tttatgggct ggggatgaat gtgagctatg atcagcaggg gcgtgcgcgg ttgaatcatt
     1021 cgggcgggtt cgccttgggt gcggggacgc atgtaaatat ggtgccgagt gagcagttgg
     1081 gcattgtcgt tctcacgaat gcgtatccgg tgggtcttgc cgaggggttg gggtttactt
     1141 tcttggactt cgcgctgtac ggcaagttga catacgattg gcttgcgctc ttcaagaaga
     1201 tattttccga tcctgcagcc attggtactg tggtcggcac cgactattcc aaaccgcctg
     1261 cgtcgccttt agccgcgttg aagagtgacg catatgttgg cgcctacgca aacgatttct
     1321 ttggggacat tgaaattgtt gccaaagatg gcggacttgc aattgtcgca ggcccgaaca
     1381 aaatgacgtt cccgttgaaa cactatgatc gcgacatttt cacgtacgag accacgggcg
     1441 agaacgcggt cggcacgaca ggtgtgtact ttacgatcgg cgcggacggc aaggcgaaaa
     1501 cggtgctcgt cgaaaatctc aacgtacata agcaaggtac cttcactcgc aagcaagcgc
     1561 caaccgacac agcgcaagac tagaacacgc cgccagcaga tcggcctgac gattccgctg
     1621 aacgtgctga tgcgggcgga tagggtgatt aaggatgcgc cgagataaac cggtatggag
     1681 tagcggatga gagagccgca caagaaagga gtagcgcacc attttggccc cgagtcatgc
     1741 gcctgggtcc aaaaggacgc gggcggagcg ttgacagggg tacccacagg cgaggtattg
     1801 agctccgaaa tcaccacttc ggcgtgccga ccttgtcttg atatagggaa ggctacatga
     1861 agggcagcga taggcgcgag cggtcctttg acaccgcgga gtcagagacc ctaggcatgt
     1921 ggggaaactc tatgcgcgag aaccgggagg ccccgcagac gcccaccatc gatggagtgg
     1981 gacggtcgaa gaaggctaaa ggccgtaatg tcgacgtgca cgtttgcgga gagtcggacg
     2041 gcctcgtagt accaacgaag cggacaaaca aagccgacgc ggaagagtgg gcagcggagt
     2101 ccgcagagga caggaggtcg accaagggga acgcaccaca gacaatcttg cgccggacac
     2161 agaaccgggg aagtcacagg gtttgtggcg cgtacggcaa gcggcaagaa gggatagcat
     2221 cggtatccca acgggcggct ggccgtcaga tgaacccgaa aatcttagag gggaagacct
     2281 gcccaacgga cagcgagagc tttcagtaat cggctacttt caaatcgttc cgggggtagg
     2341 ggatggtccg gagtctagcg ctggtgctag tcgaaaagcc accacgatgt cgacatcagg
     2401 attcaggcgg acgacctcgt tggtgaccgc ctcgcggacc ttgtccatgt cctgaagtga
     2461 gtgcgtggga ccttcgaaga acgacaccat cacaaagacc cgccgtcctg ccttgcgcac
     2521 ccgcacccat tcaacgccct taaggtgaaa ttgctcggcc acggcagccg ccgattcctc
     2581 cacagcgttg accgccccgg catacgggtt agcgtcgacc agatcgctga tgctttcgcg
     2641 caggatgtcg tacggctttt tgagaaagaa cagcgataac acaaggcaca cagcgggatc
     2701 aatgtaggcg gcggcctcga tcttccccat gcggccaagg acgatgctgg ctacaaacgc
     2761 tccacacacc cctaaggcga gccaaccgcc gaggatccaa agatcggcgt tggctaggac
     2821 tagcggcgag ccggcacgtt tgccgacgct gcgcatccag agcccaaaga tcacggagat
     2881 gaaaaaactc gcggcggaat agatgacgat caagtacggg gcttcgaacg ggtccgggtg
     2941 catgatatct tgtgcggagg agaggatggc gctcgcgcag atcccggtca tgagaatgcc
     3001 ttccaccgta gtcatgagcg gctcgtattt cgcgtagccg aactggtagc gctggttggc
     3061 tggctcagcg accttgcgtg cgacgaagat ggccaggact gacaccacga gatcgacgat
     3121 cgcggacccc gcctctaggg ccatcgtctc cgagtcggaa tagattgcga tgacgatagc
     3181 gaccgctgtg aacaggatga cgagaaggag ctgtagccag agagagagaa gctcttgtga
     3241 cctggcgcga tcgctcatgt tacctcctcg gcgtcgggta tgtccgccgg cgattaacta
     3301 tgcgacgcta cgtggtgtct tttttttcga agatgaggac tgagccggtc ttccaagcga
     3361 actcaatacg ggattgcaag gccccatcga agttccgcgg ccaatgagtt aaggtcgggc
     3421 aaaccttgtg gaccttttgg accaccgcct cctctcagct gttgcagaat agttccacca
     3481 ggcgacacaa gctccgggtt ttagttgcct tcgtcattga cggacgcgac tgtctttctt
     3541 cctgcagtat gctcctcaac atgcgagaga tacacatggt gttgcgacag tttatgacga
     3601 atctccagcg actcctctct aatgattcga gcattatcaa gaagcgtatc ggtgagttgt
     3661 tttgatacca gcataactcc gttgcaatcc tcgctttgac ttgatttatg cccttctcgt
     3721 acacccccgt ggaaacccga aaggtatcac actccatttg ttagacacaa catcttattt
     3781 acaggctgtt gtaccaaagt gattatgtca ggggttaatg gcaacgtaat tatgtcaggg
     3841 ggtggagatg acaaccctga cgatgaagga agagaagcgg ctagagataa tacagagagt
     3901 gtttcgaggg gagctgacag tggtggagca caaaacaaac acagccggga tcggccctcg
     3961 ccttgcgggc ggcctgtgct gatactaata tccgcaaagg ccgttctata tcttactggt
     4021 taggaccgca tgccgcgcaa ccgcacgaca atcgaagcag cggccggacg ttccgtccag
     4081 aaggaatggg atggcgaact cggtgaggat cgcagccaca tagtcttagc gccttgatcg
     4141 ccgcccacaa tttgctcaag ctgccaagcg tggcgacatc aaacgagttg cttcatggac
     4201 gcggaatagt cgctgatacc gaggtatcgg ccgataccat cgtccaatcg accgggcact
     4261 tgagggacga aaggaggtcc acatgaaaac ggctcgaatc catggcggta gtcctgctga
     4321 tcgtcggcag cggtttggca ctgcacgaag cgctgtcaca gcagccggga atcaagcgca
     4381 ccgatctcca gcggcacgat ctggcgtgcc actcgcggat ctcgagtcga acagtcatcg
     4441 ggtgcgtgcc cggacccact gggttctaag ctgcagcgga tgggttcact tacgcatatt
     4501 gaaggagaca ccctatgaga cggattcttt tcgtgctggt tttagttatc gcggtggc
//
LOCUS       DASSWZ010000334         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_225579, whole genome shotgun sequence.
ACCESSION   DASSWZ010000334 DASSWZ010000000
VERSION     DASSWZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225579"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /gene="obgE"
                     /locus_tag="VFN58_08015"
     CDS             <1..1023
                     /gene="obgE"
                     /locus_tag="VFN58_08015"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET8917972.1"
                     /translation="VKIRVIAGDGGRGCISFRREKFVPRGGPNGGNGGKGGNVIALAD
                     PQLTTLLDLRYQKQYQAGRGEHGLGKDQHGRTGEDRVIKVPVGTIIRDATTGELIGDL
                     KEANQQVVVAAGGRGGKGNAHFVSSTHRSPRFAQPGEPGEDKELEIELRLLADVGIIG
                     LPNAGKSTLIAAISAVRPKIADYPFTTLVPNLGVVGYGEGKSFVVADIPGLIEGAHAG
                     HGLGHKFLRHVTRTSLLIHLLDASNIDEKNPLAEWKIVNQELELFDPELAKKPQIVVA
                     NKIDLPAGKNHAKLLAKKLPNEHQPLHSISAATREGVRTLVQYIGMKLEEIKQQGEMG
                     GDAAGL"
     gene            1001..2140
                     /gene="proB"
                     /locus_tag="VFN58_08020"
     CDS             1001..2140
                     /gene="proB"
                     /locus_tag="VFN58_08020"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET8917973.1"
                     /translation="MATQLDYKKEILRRARRVVIKIGSQILSSQTGIEEGRVKGLVHG
                     LAELHNQKKDIIVVSSGAVAAGMTRLGVKERPKTIPQKQALAAVGQIKLMALYERFFS
                     RFEKNVAQVLLTHEDLANRQRYLNAKHTFQMLLESSIIPIVNENDTVAVEEMKFGDND
                     HLSALVATLLEADLLVMLSDVQGVYDRDPRLYNDAEGISLITDIKEIARKISGESQSE
                     FGTGGIATKLDAAEKAAAAGIPTLISSGLQTGALALVFDATAEVGTLIIADENRMTIR
                     KHWIAYNLKPAGEIVVDQGAHDAVVERGKSLLPSGLKEIRGSFGVGECVRCLDPQGRE
                     FARGLVNYNAQELNQIKGLHTSKIDKVLGYKAYDEIIHRDDLVIL"
     gene            2144..>2763
                     /locus_tag="VFN58_08025"
     CDS             2144..>2763
                     /locus_tag="VFN58_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET8917974.1"
                     /translation="MSIKNESIKEEAIRLAKQAKAASRQLAPISAAEKDRALHLMADQ
                     LEAQNDYLVAENKKDLDAAERAGISEPIVDRIALNPGRVQAMAKGLRDVAALPDPVRE
                     VVKMWRRPNGLQVGKMRIPLGVIAIIYEARPNVTADAAALCLKSGNAVILRGGSEAHF
                     SNQAIGQILRKACAQTRVPREAVQIVQTKDHAFVQELLQLEDYIDLV"
BASE COUNT          699 a          663 c          843 g          558 t
ORIGIN      
        1 gtaaaaatcc gtgtcatcgc cggtgacggc gggcgcggtt gcatcagttt ccggcgtgaa
       61 aagtttgttc ctcgcggcgg ccccaatggc ggcaatggtg gaaagggtgg aaacgtcatt
      121 gcgttggccg acccgcagct cacgacactc ctggatttgc ggtaccaaaa gcaataccag
      181 gcgggtcgcg gcgagcatgg attgggtaaa gatcagcacg gaagaacggg cgaagaccgg
      241 gtgattaaag ttcccgtcgg aacgatcatt cgggatgcca caacaggtga attgatagga
      301 gatttaaaag aggcaaacca acaggtcgtc gtggctgccg gtggccgcgg gggcaagggc
      361 aacgcccatt tcgtttcgtc gacccatcga agtccacgct tcgcccagcc gggtgagccg
      421 ggagaagaca aggaattgga gattgagctc agattgctgg ccgacgtcgg cattattggt
      481 ctgcccaatg ccggaaaatc aaccttgatc gcagcgattt ccgcggtgcg gccgaagatt
      541 gccgactatc cgtttacgac tttggttcct aacctgggcg tcgtcggcta tggggaaggg
      601 aagagtttcg tggtcgccga tattcccgga ctcatcgaag gcgcgcacgc aggccatggt
      661 ttgggacaca agtttttgcg gcatgtgacg cggacgagtt tgttgattca tttgctggat
      721 gcatcgaata tcgatgaaaa aaatcccctc gccgaatgga agatcgtcaa tcaggagctc
      781 gagctctttg atccggaact ggcaaaaaaa ccacagattg ttgtggccaa caagatcgac
      841 ttgccggcgg gaaaaaacca cgcgaaactt ctggcaaaaa aattgcccaa tgaacaccag
      901 ccgctgcaca gcatatccgc cgcaaccaga gaaggggtgc gcacgttagt gcagtacatc
      961 ggaatgaagt tggaagagat aaaacaacaa ggtgagatgg gtggcgacgc agctggatta
     1021 taagaaggag atcctgcgcc gtgcccgccg ggtcgtcatt aagatcggga gtcaaatact
     1081 ctcctcacaa accgggatcg aagaggggag agtcaagggg ttggtgcacg gcctcgcgga
     1141 gctccataat caaaagaaag acatcattgt ggtaagctcc ggcgccgtag ccgctggaat
     1201 gacgcgtctc ggtgttaagg aaagacccaa gaccattcca cagaaacagg cgttggcagc
     1261 ggtgggccag atcaagctga tggcgttgta tgagcgcttc ttctcgcgct tcgagaagaa
     1321 cgttgcccag gtactgttga ctcacgaaga cttggcgaat cgccagcgct atttaaacgc
     1381 gaagcatacg tttcaaatgt tgctggaatc gtccatcatt ccgatcgtca atgaaaacga
     1441 cacggtggcg gtggaggaaa tgaaattcgg ggacaacgac catttatcgg ctcttgtggc
     1501 gaccctgctt gaggcggatc tcctggtcat gctgagcgat gttcagggag tttatgatcg
     1561 agatccgaga ttgtataacg acgccgaagg gatttctttg ataaccgata ttaaagaaat
     1621 cgcgcgaaaa ataagtggtg agagccaaag cgagtttggc accggcggca ttgcgacgaa
     1681 gctggacgcg gcggagaaag ccgccgccgc aggaattccg accctgatca gtagcggtct
     1741 gcaaaccgga gcgcttgcgc tggtatttga tgcaactgcg gaagtcggca cactcattat
     1801 agccgatgag aatcgtatga ccattcggaa acactggatc gcctacaatc taaagccggc
     1861 aggcgagatc gttgtcgatc agggcgccca tgatgctgtg gtggagcggg ggaaaagctt
     1921 gttaccctcc ggtttgaagg aaatacgcgg ctcgtttggt gtgggtgaat gtgtgcgctg
     1981 tctcgaccca cagggacgag agttcgcccg tggcttggtc aattacaacg cccaggagtt
     2041 gaatcagatc aaaggattgc atacaagcaa gattgacaaa gtcctgggct acaaagctta
     2101 cgacgagatt attcaccggg acgatcttgt gattctatag agcatgtcta ttaagaacga
     2161 atccataaaa gaagaagcga tccgcttggc taagcaggct aaggcggcgt ctcgacaact
     2221 ggcgccgatc tcagcggcgg aaaaggatcg cgcgctccat ttgatggcgg accagcttga
     2281 ggcgcaaaac gattaccttg ttgcagaaaa caagaaagac ctagacgcgg ctgagagggc
     2341 gggaatttca gagccgatag tggaccggat tgcgcttaat cccggtcgtg ttcaggcgat
     2401 ggcgaaaggg cttcgagacg ttgccgcctt gcctgatccg gtgcgagagg tcgtgaaaat
     2461 gtggcggcga cccaacgggc tgcaagtcgg caagatgcgg atccccctag gcgtaatcgc
     2521 tatcatttat gaagcgcggc ccaacgtgac ggcagacgcc gcggcgctgt gcctaaaatc
     2581 gggtaatgcg gttatcctga ggggtggcag tgaggcgcat ttctctaacc aagcgatcgg
     2641 ccagatctta aggaaagctt gcgcgcagac gcgcgtgccg cgggaagcgg tgcaaattgt
     2701 tcagaccaaa gaccatgctt tcgtccagga gctgctccaa ctggaggatt acatcgatct
     2761 cgt
//
LOCUS       DASSWZ010000335         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_353001, whole genome shotgun sequence.
ACCESSION   DASSWZ010000335 DASSWZ010000000
VERSION     DASSWZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353001"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1271)
                     /locus_tag="VFN58_08030"
     CDS             complement(261..1271)
                     /locus_tag="VFN58_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917975.1"
                     /translation="MIHACLWPGLSWGQAAYYQGKTLTILRGGSPGGYGDFQARALIP
                     YLKKYIPGEPTIVIEHKQGAAGRTAVNHIYSGAKPDGLTIGAVGNVLVSGPILGLPGS
                     NYDLDRLIYLGSTESGSPTAFVTRKEAGLDSLEKLRAATGIRIGAHSVGHITYINGRL
                     AAYLLGLKEPKFVVGYSSPERDVALLQGEVDGGSMSRATMQQDPSLMEKVYFHTVITV
                     PKGRYVPPYSRDIPEIDSFAKSEKEHRLLSLYRAFDYPRWPYFLPPGTPKELVNILRE
                     AMAKAFKDPGFAKEFKNLTGADPSPIPAEAIEAAIKELPRDPETIALYKHLADHRPMP
                     AR"
     gene            complement(1708..2799)
                     /locus_tag="VFN58_08035"
     CDS             complement(1708..2799)
                     /locus_tag="VFN58_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET8917976.1"
                     /translation="MRGTQDRQASMLALFSIEARIPREHPLRRIKRMADQQLLRLSPV
                     FNRMYSNVGRPSVAPEKILKSLLLIALYSVRSERQFCEQLGYNLLFRWFLDMELEEES
                     FDATVFSKNRERLMEHEVGRLFFDAVVREARDAGLMSAEHFTVDGTLIEAWASLKSFR
                     PKGEEKSGDKDQGDPGNPSVDFHGEKRKNDTHQSTTDPDSLLIRKGPGKEAKLSYAAH
                     ALMENRNGLLVDLAVSQATGTAEREAAKSMIKRQKRKGVRIKTVGADKAYDTEGFVDF
                     LRSRKIVPHVAANTERRGGPAIDGRTMRHQSYSISQRIRKRVEEIFGWMKTVGGFRKT
                     HYRGTDRTRLAAWWVGAAYNLVRMAKLVA"
BASE COUNT          682 a          856 c          760 g          713 t
ORIGIN      
        1 cgctctaagc ctccagaccg cgctatttat accgtcttct atcggttaat caatggtttg
       61 cgccggtccg acccgagccg cttggccgag ccagcttcca gtcggtttgg aatctcggga
      121 gaccctttga ggaggccaaa ctatcaagat cgcgtctgcc tatgaaaaaa tcaaacggta
      181 tcgccgcccg cccgcgggat ttggccccct ctcgggtgag ccataaagtg ggtaacaaag
      241 tctagctcga atctcacacg tcaacgcgca ggcatggggc ggtggtccgc caggtgcttg
      301 tacaacgcga tcgtctccgg atcgcgcgga agctccttga tggccgcctc gattgcctct
      361 gctggtatgg gcgaaggatc ggcacccgtg agattcttaa attctttggc gaaccccgga
      421 tctttgaatg cttttgccat ggcctcgcgc aaaatgttga ccagctcttt gggcgtgccg
      481 ggcggcaaaa aatagggcca gcgcggatag tcgaacgccc gatacaagct cagcaaccgg
      541 tgttccttct cgcttttggc aaagctatcg atttcgggaa tgtcgcgcga atagggcgga
      601 acgtagcgac ccttggggac ggtgatgacg gtgtgaaaat aaactttttc catcaacgac
      661 ggatcctgtt gcatggtcgc gcgcgacatc gacccgccgt ccacttcgcc ttgtagcagt
      721 gctacatccc tttccgggga tgaatagcca acgacgaatt ttggctcctt caatcccagc
      781 aaataggcgg caaggcgccc attgatataa gtaatatgtc ctaccgaatg ggctccgatc
      841 cggattcccg tggcggcacg gagcttttcg aggctgtcca gccccgcctc ctttcgagtg
      901 acgaacgcag tcggactgcc gctttccgtg gagccgagat agatcagcct gtccaaatcg
      961 tagttgctgc cgggcaggcc gaggatcggc ccgctgacca agacgttgcc cacggcgccg
     1021 atcgtcaacc catcgggttt agctcccgag taaatgtgat taacggccgt tctacctgcg
     1081 gcaccctgct tgtgctcgat aacgatagtg ggttccccag ggatatattt tttcaggtaa
     1141 gggatcaggg cccgggcctg gaaatcgccg taaccgcccg gtgatccgcc gcgcaggatg
     1201 gtgagcgtct tcccttgata gtaggcggcc tgcccccagg ataaccccgg ccagaggcaa
     1261 gcatgaatca agaaaagagc ggcaagaaaa ctcaggcatt cgaagcgatt ctttcttagt
     1321 tccggctcta taggttggtc ccccccttcc aaagaaaact atcagggcca cgtgaaaaaa
     1381 cggtgtgcgt ccttagctgg catttgcacc atagataaaa aaaattcaag acggttttga
     1441 ggttttatct taaaacgtgc tgccgaaatt cacgtatgat cacgggaaat aaacagttgc
     1501 tcggcaatca agggagctct cgacacaata agctacacga taacctagca ggttgctgaa
     1561 aaatggactt taagaagaga aatttgtatg cggggagaga attttggagc gtcatcaagc
     1621 acgtaggatg aaatgatgag cgtggaatgc actacgaact gggttttcaa ctctgattcc
     1681 atcatttctc agttactttt aaaccgctta ggccaccagt ttggccatgc ggacaagatt
     1741 gtacgcagct ccgacccacc aagccgccag tcgggttcta tcggttcccc tgtagtgggt
     1801 ctttctaaat cctcccacgg tcttcatcca accgaaaatc tcttctactc gttttcgtat
     1861 ccgctgactg atgctatagc tctgatggcg catggtacga ccatcaatcg caggtccgcc
     1921 tcttcgctcg gtattggctg ctacatgagg gacgatcttt cggcttcgca aaaaatccac
     1981 aaacccctca gtgtcatagg ctttatcggc tcccacagtt tttatcctca cgccttttcg
     2041 tttctgacgc ttgatcatac tcttagccgc ttcccgctct gcggtgccag tggcctgact
     2101 gacggcaaga tcaaccaaca ggccgttgcg gttctccatc aaggcatgcg ccgcgtaact
     2161 cagcttggct tctttgcccg gccctttgcg gatcagcaga ctgtcgggat cggtggtgga
     2221 ctgatgggtg tcgtttttgc gcttctcgcc gtgaaagtcc accgagggat tacccggatc
     2281 tccctgatct ttatctcccg atttctcttc gcctttgggt cgaaagctct tcaggctagc
     2341 ccatgcctca atgagagtac cgtccaccgt aaaatgctct gcactcatca gtcccgcgtc
     2401 tcgggcttct cggaccaccg cgtcgaagaa caaacggccc acctcgtgct ccatcaatcg
     2461 ctcgcggttc ttactgaata ccgtggcatc aaaactttct tcctcaagct ccatatccaa
     2521 aaaccagcga aacagaagat tatagcccag ttgctcgcaa aactgccgct cgcttctcac
     2581 cgagtagagt gcgatcaaca acagcgactt caggatcttc tcgggcgcca ccgagggccg
     2641 tccaacgttg gaatacatcc gattaaatac cggcgacaat ctcaagagtt gttgatccgc
     2701 catccttttg atccgtctca atggatgctc cctgggaata cgggcctcga tagaaaacag
     2761 cgccaacata cttgcttgtc tgtcctgggt tccgcgcatc aatgcctccg aagattcttt
     2821 cggccccata ctaacatacg cggtgattct cagagtagaa aaaatgtcca aattaggagt
     2881 ttttcagcag cctgctaggc aactccttgg cgcagcaggg taacttcgaa gcggccggca
     2941 aacaactgca gcacgcgttg caaataaatc ccggcaatgc catggccgct tacgacttag
     3001 gcaacgtccg t
//
LOCUS       DASSWZ010000336         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_494798, whole genome shotgun sequence.
ACCESSION   DASSWZ010000336 DASSWZ010000000
VERSION     DASSWZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494798"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /gene="rsmB"
                     /locus_tag="VFN58_08040"
     CDS             complement(<1..1330)
                     /gene="rsmB"
                     /locus_tag="VFN58_08040"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET8917977.1"
                     /translation="MIASNEKRRKGVRQLASEILFKVDSRKAYADVLLDHSLKDAALS
                     DRDRALLTELAYGTLRWRGKIDAHLNLYLRDPLEDSDPFIRNLLRVSFYQLIFLDKIP
                     DYAAVNEAVRLAKAHGGDKVAGFVNAVLRCFLREKDRAAEPQRNNNWQASLAIEHSHP
                     QWLVEKWLDYFGRDETVALIKANNEVAPLVLRVNSCRSSRKALLALLSKSGVSAVATR
                     WSPVGISVKSSSPVNQLPGFQEGLFQVQGEASQLVSYLLSPEKGERVLDACAAPGGKT
                     THIAELMADTGELIALDKSEKGIEKIRENVARLGLASVCAAKTDVSLRLPVEFRSPYD
                     RILVDAPCSGLGTLRSHPEIKWHRNETDIKRLSHLQENIVDRVVHYLKPGGVLVYSTC
                     TLNKDENENIIEDFLEHHKEFAVDDASGYLPGDASSLVRGSHYMTLPHRHN"
     gene            complement(1314..2228)
                     /gene="fmt"
                     /locus_tag="VFN58_08045"
     CDS             complement(1314..2228)
                     /gene="fmt"
                     /locus_tag="VFN58_08045"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET8917978.1"
                     /translation="MGTPQTAAFTLEHLLQGPDPVIGVVTQPDRPAGRGQKTVLSPVR
                     KLAESRCLSVVAPEKVRDPGFLETLKRWNPQLIVVVAYGRILPTAVLELAPHGCLNVH
                     YSLLPKYRGAAPAVWTILNGEEKGGVTTMKLAEKMDSGPVYLQKEVTLAPDETTSSLQ
                     AKLTPIGARLLLETIRRLKDGSLEPQAQNEADASFAPIIKKEDGLIDWNEPATKIERR
                     VRGFDPWPGAYSHVDGKLLKIHRAKVVAANTKGSPGEVMRADAGGLWVATSSNVLGLE
                     EVQLENRKRLSGVEFLRGARIKPGDRFQ"
     gene            complement(2305..2844)
                     /gene="def"
                     /locus_tag="VFN58_08050"
     CDS             complement(2305..2844)
                     /gene="def"
                     /locus_tag="VFN58_08050"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET8917979.1"
                     /translation="MAILEVLKYPDPRLSRIAQPVENINGEIVRLVNDLLETMYAAPG
                     VGLAANQIGAFHRIVVLDVDHENPQKNVYKLINPTIVRSEGEILWEEGCLSVVDFTAE
                     VRRAARVEVAALDENEKEIKIEAEGLLAVALQHEIDHLDGKLFIDRISRLKRDLYTRK
                     RKKMLRTGATPSESRHVMI"
     gene            complement(2857..>4409)
                     /gene="priA"
                     /locus_tag="VFN58_08055"
     CDS             complement(2857..>4409)
                     /gene="priA"
                     /locus_tag="VFN58_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET8917980.1"
                     /translation="RAGRGSLILIPEISLTPQLIDRVNARFPGRVGILHSALSGAERW
                     TQWWRIVRGNVDVVVGARSAVFAPLANLGLIIVDEEHDPSYKQEEGLRYNGRDVAVVR
                     GKLSACPVILGSATPAIESYQNSLDGRYRLLEMTQRVQRRPLPFIETIDLRSKFNRSR
                     QPNAGKVPLQTDSPSVQQSGEHRLISDRLATFLRKNYQASRQSLIFLNRRGFSNYLQC
                     ALCGHVLRCSYCSVTLTLHKKQKSVCCHHCNFRRAATALCPECGNLTLSGVGVGTEQI
                     EEALRRLVPEARIARMDRDTTSKRGAHEELIRSWEKGETDILVGTQMITKGHDVTGVT
                     LVGALLADLSLNLPDFRSGERTFQLLSQVAGRSGRGDEPGTVIIQTYAPDHYAIQHLI
                     HHDYKGFFAAEIDFRRALNYPPFSRLVCLRLEGPKFEEVEKKARMLGATLHDKTIHNA
                     RLREKIEILGPAPAPIEKLRNRYRWQLLLKGKQSSALLELAKQAREALPRSRSVRLHI
                     DVDPYNML"
BASE COUNT          955 a         1217 c         1188 g         1049 t
ORIGIN      
        1 tgttatgccg atgaggtaag gtcatgtagt gactgccgcg aaccaaagag ctggcgtctc
       61 cgggaagata gcctgaggca tcgtccacgg caaattcttt atggtgttct aagaaatctt
      121 caatgatgtt ctcgttttca tccttattga gtgtgcaggt cgagtagact aagacgccgc
      181 cgggcttgag gtaatgcacg actcgatcaa caatgttctc ctgcaagtgg ctcaatcttt
      241 tgatgtcggt ttcatttcga tgccatttaa tttcgggatg agatctcaga gtgccaagcc
      301 cgctacaggg cgcgtcaaca agaatgcggt cgtagggact gcggaattcg acgggcaatc
      361 gaaggctgac atcggttttg gccgcacaga ccgaggccaa ccccagcctc gcaacgtttt
      421 cgcgaatctt ctcgatccct ttctctgact tatcaagcgc gatcaactcg ccggtgtccg
      481 ccataagctc agcgatgtga gtagtcttgc cacccggcgc ggcgcaagca tcgagaaccc
      541 gttcgccctt ttcaggtgaa agaagatagc tgacgagctg cgatgcctct ccttgaactt
      601 gaaacaaacc ttcctgaaaa cctgggagct ggttcaccgg agaactcgac tttaccgata
      661 tccccaccgg cgaccatcga gtggcgacag ctgaaacgcc gctctttgac aacaaagcga
      721 gtaacgcctt tctgctgctc ctgcaagaat ttacacgtag caccagcggg gcgacctcgt
      781 tgttggcttt gattagcgct acggtctcgt cgcggccgaa atagtcaagc catttctcta
      841 ccaaccactg gggatgagag tgctcaatgg ccaggctcgc ctgccaatta ttattacgtt
      901 gaggttcggc ggctctgtcc ttctctctca ggaagcagcg cagaactgca ttgacaaacc
      961 cggctacctt gtccccgcca tgggctttgg caagccggac ggcttcattg acggctgcgt
     1021 aatcggggat cttgtcgaga aaaatcaact ggtaaaagga aacgcgaaga aggtttctaa
     1081 tgaaaggatc gctatcttct aaagggtccc gtaggtaaag attcaggtgc gcgtcgatct
     1141 tgccgcgcca gcggagggtt ccgtaagcaa gctctgtaag cagagcgcgg tcgcgatcgg
     1201 aaagagccgc atctttgagg ctgtggtcga gaaggacatc ggcgtaggct tttctagaat
     1261 cgactttaaa gagaatctcg cttgcgagct gacgaactcc tttgcgtctt ttttcattgg
     1321 aagcgatcac cgggtttaat cctcgcccct cttagaaact cgacaccgga aagtcttttt
     1381 cggttttcca attggacctc ttccaatccc aagacattgg agctcgtggc gacccaaaga
     1441 ccgcctgcgt cggctctcat aacttcgcct ggactcccct ttgtgtttgc tgcgacaacc
     1501 ttcgctcggt gaatttttag taactttccg tcaacgtgac tgtaggcgcc gggccaagga
     1561 tcgaagcccc gcacccgccg ttcgatcttt gttgccggtt cattccagtc tatcaaaccg
     1621 tcttctttct ttatgatggg agcgaaactg gcatcagctt cgttctgcgc ttgtggttcg
     1681 agggagccgt cttttagtcg tctaattgtt tccagcagca gccgcgcgcc gataggcgtg
     1741 agcttcgcct gcagcgaact cgttgtttca tccggcgcca gcgtgacttc cttttgcaaa
     1801 tagaccggtc ccgagtccat cttctccgct aacttcatcg ttgtgacccc gcctttttct
     1861 tcaccgttaa ggatcgtcca cacggcaggc gccgcaccgc gatacttcgg cagcaacgaa
     1921 tagtggacgt tgaggcagcc atggggcgca agttccaaga cggccgtagg aagaatgcga
     1981 ccataggcga ccacaacgat gagctgagga ttccaacgct tcagcgtttc caggaagcct
     2041 ggatcgcgca ctttctccgg cgccacaacg gataggcagc gactctcggc tagtttacgg
     2101 accggcgaaa gaacagtttt ctgacccctc cccgcggggc gatcgggttg agtgacgacg
     2161 cctatcacgg gatcaggacc ctgcagcagg tgctccaacg tgaaagcggc ggtttgcgga
     2221 gttcccatga aaacgatgcg ccaggcaggg gacatacaag cggtttgaat cccgatcgtg
     2281 gggaaaaccc aaaagccgtt acttctagat cataacgtgc cgagattctg atggggtcgc
     2341 gccggtgcgg agcatctttt tgcgtttacg ggtatagaga tcgcgtttta gtcggctgat
     2401 acggtcaatg aagagtttgc cgtccaagtg atcgatctcg tgctgcaagg cgacagccag
     2461 gagaccctct gcttcaatct tgatttcctt ttcattttcg tcgagagccg caacctctac
     2521 ccgggcggcc cgacgcacct cggcggtaaa atccacgacg ctcaagcaac cttcttccca
     2581 gagaatttcg ccttcggacc ggacgatggt gggattgatc agcttgtaaa cgtttttctg
     2641 cgggttctca tgatcgacgt ccaggacaac gatgcgatgg aacgccccga tttgattcgc
     2701 tgccaatccg acaccaggcg ccgcgtacat cgtttcgagc aggtcgttga ccagccgcac
     2761 aatctcgccg ttgatgttct ctactggctg ggcgattctg ctcagccgtg gatcggggta
     2821 tttaagcacc tccaaaatag ccatgtctat cagatttcat agcatgttgt agggatcgac
     2881 atcaatgtgg agacgaacgc tacgtgatcg tggtagcgcc tcgcgtgctt gtttggcaag
     2941 ctcgagcaaa gcagaactct gctttccctt taatagaagt tgccagcgat agcgattgcg
     3001 cagcttttcg atgggcgccg gtgccggccc aagtatttca attttctctc ggagccgggc
     3061 attatgaatg gttttgtcat gcaatgtcgc gccgagcatt ctggccttct tttcgacctc
     3121 ctcgaatttt ggaccctcta atcgaagaca gacgagtcga ctgaaaggcg ggtagtttaa
     3181 ggcgcgccgg aagtcgatct ccgcagcaaa gaatcctttg taatcatggt ggatcaagtg
     3241 ttgaatcgcg tagtgatccg gcgcgtatgt ttgaatgatg acggttcccg gctcatcgcc
     3301 tctgccggat ctgcccgcga cttggctcaa cagctgaaag gttctctcgc cggagcgaaa
     3361 gtccggcaag ttgagcgaca gatcggcaag caacgcgcct actagtgtca cgccggtcac
     3421 atcgtgtcct ttggtaatca tttgggtacc gactaggatg tcggtctcgc ctttttccca
     3481 actccgaatc aattcctcgt gcgcccctct tttgcttgtc gtatctcggt ccatgcgcgc
     3541 tatgcgcgcc tcgggcacaa ggcgacgcag cgcctcttcg atctgctcgg ttccgacacc
     3601 gacacccgaa agagtcagat tgccgcattc gggacaaagt gccgtggcgg cgcggcgaaa
     3661 attgcaatga tgacaacaga cgcttttttg cttcttgtga agggtaaggg tcacgctgca
     3721 ataggagcag cgcagcacat gaccgcaaag agcgcattgc agataattcg agaatcctcg
     3781 ccggttgaga aagattaggc tctgccgcga agcttgataa ttctttctca gaaacgtcgc
     3841 aagccgatcc gagatcagtc gatgctcgcc tgactgctgc accgatggcg aatctgtctg
     3901 caatggaact ttgccggcgt ttggctgccg gctccgattg aattttgatc taagatcgat
     3961 ggtctctatg aagggaaggg ggcgccgttg taccctctgt gtcatctcca ggagtcggta
     4021 tcttccatcc agcgaattct gatagctctc aatggccggc gttgccgacc ctaagatgac
     4081 cgggcaggcg gacaacttgc cacggaccac ggctacgtcg cgtccgttgt atcgaagtcc
     4141 ctcctcctgc ttgtaagaag gatcatgctc ctcgtcgacg atgataagtc ccaggttcgc
     4201 gaggggcgca aaaacagctg agcgtgcacc cactacgacg tcaacgttgc cgcggacaat
     4261 gcgccaccac tgggtccagc gctccgcccc cgaaagagca ctgtgcaaaa tcccgactct
     4321 accaggaaag cgtgcattga cacgatcgat cagctggggt gtcaacgaga tctccggaat
     4381 cagtataaga ctgccacgcc cggctcgcc
//
LOCUS       DASSWZ010000337         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_735025, whole genome shotgun sequence.
ACCESSION   DASSWZ010000337 DASSWZ010000000
VERSION     DASSWZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735025"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..778)
                     /locus_tag="VFN58_08060"
     CDS             complement(32..778)
                     /locus_tag="VFN58_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET8917981.1"
                     /translation="MTRNSLAIIGEGRLSQELLELSRGTGLDAVLLSDLAELTPTTPL
                     VIETSSTLVEKKKSLLQRLDLALPGPAVIITSCLGFATSLMASWTAKPERIVGFATFY
                     PFKDRKVVELAGGIRTTEGALQSAEQFFKSIGKETVRVKDMPGLTFPRILSLIINEAA
                     RSLEEGVATAEEIDLAMRLGVNYPQGPLRWADQIGLDEVLAVLEGLQRETGDDRYRPA
                     PLLKKLVIAGFLGETRGKGFYSYNEGQVKP"
     gene            complement(765..1631)
                     /locus_tag="VFN58_08065"
     CDS             complement(765..1631)
                     /locus_tag="VFN58_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016741463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET8917982.1"
                     /translation="MAEQSVKIGVLGAGTMGAGIAQVAAQAGFETLLYDIKQEFIEAG
                     LGRIRDFLQGSRQRGKISPDDEKQILDRLRSTTKLETFEESTLIIEAVPEKLELKRDI
                     FKQLDGICGPETLLATNTSSFSVTAIAADTQHPERVLGLHFFNPPPLMALVEVIQGDR
                     TTLEVIARATELMRQMGKTPARAKDTPGFIVNRVARPFYNEALRILGDGEATIETIDR
                     IMRLAGNFRMGPFELMDLIGHDVNFAATESLYRSFFEDPRFRPSPIQHKMVMGGNLGR
                     KTGRGFYVYDKK"
     gene            complement(1717..2496)
                     /locus_tag="VFN58_08070"
     CDS             complement(1717..2496)
                     /locus_tag="VFN58_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8917983.1"
                     /translation="MPYETILYEKENGVATITLNRPQALNAFVPQMNKEVLEVLKEGE
                     RDKEIRCFVITGAGRAFCAGQDLKGRTPEQKGSLGASLREKYNPMIRQIRQMEKIVIG
                     AVNGVAAGAGCNLALACDLRVASEEAKFIQSFVRVGLAPDSGGSFILPRLVGLSKAME
                     LLLLGETIDAHEAQRIGLVAKVFSAAEFAKSTKEIAARVAGAPRGIGLIKRALNHAIL
                     PDIEADLEYESHLQEIAGRSTDYDEGVRAFLEKRPPVFTGK"
     gene            complement(2511..3284)
                     /locus_tag="VFN58_08075"
     CDS             complement(2511..3284)
                     /locus_tag="VFN58_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET8917984.1"
                     /translation="MQFDNVIVEKDASIAVLTLNRPQQLNALSYDLVKDLSLAMQELD
                     HDDEVRVMIVTGGEKVFAAGADIKEMADRGPFDARIQERLAYRDRINRITKPVIAAVS
                     GFALGGGCELAMSCDIVVASETARFGQPEVNLGIIPGSGGTQRLTRTLGKYRAMEIVL
                     AGETISATDAERFGLVNRVVPVELLLDEAKNLAKKIAAKPVLAVRAAKEAVLKSANAN
                     LDEGLEFERKSFYLLFASEDRKEGMKAFLEKRKPEFKGS"
     gene            3462..4145
                     /locus_tag="VFN58_08080"
     CDS             3462..4145
                     /locus_tag="VFN58_08080"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET8917985.1"
                     /translation="MSNRLEPTSRNYDEKLQRILKTSAKIFAEKGFHRTSVRDISRAT
                     KMSLAGLYYYFTTKEELLYLIQERCFVTLLQRWERAAVPEIDARTRIRFFAENHLSFF
                     CHNMHEMKVMAHEDESLTGEFQVKILVLKRRYVKVIMDLIRELQDQEAGKKVDLRVAT
                     FALFGMMNWIYTWYQPKRDLPLPQLMEQMLRIYFFGVLQGAAVEAPWFNSISAASSKE
                     TFSLWHNHQ"
     gene            4193..4717
                     /locus_tag="VFN58_08085"
     CDS             4193..4717
                     /locus_tag="VFN58_08085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET8917986.1"
                     /translation="MRVGDKAPNFTLKGIFRGTVSDFALQSFSGKWLILFFYPADFTF
                     ICPTEVAGFSKMAHQFRAEDAEILGVSVDSLESHRKWAEELGGVNYPLLSDDGKTVSR
                     LYGVLDEKEGVCLRGTFIINPTGEITFLVISHTNVGRSVEETLRVTKALRSERLCPAD
                     WHPGEPTGDLNLKY"
BASE COUNT         1074 a         1317 c         1225 g         1110 t
ORIGIN      
        1 gtgcgcacgg cttccacaat aactgcttcc ttcatggttt cacctgacct tcgttgtatg
       61 aataaaaacc tttgccccga gtctcgccga gaaaacccgc gatgaccaat ttctttagca
      121 ggggcgccgg gcggtaacga tcgtctccgg tttcacgttg cagaccttcg aggaccgcca
      181 agacttcatc caaaccgatc tgatccgccc agcgcagcgg accctgcgga tagttcacac
      241 cgaggcgcat cgcgaggtcg atctcctccg ccgtggcgac tccttcttcg agactcctgg
      301 cagcttcatt gatgatcaaa ctcagaatac gcgggaaagt tagccccggc atgtctttaa
      361 cacgaacggt ttccttgccg atggatttga agaattgctc ggcactttga agcgcaccct
      421 ccgtagtgcg tatgccaccc gccaactcaa cgacctttct atccttgaat ggatagaaag
      481 ttgcaaaacc cacgatgcgt tcaggtttcg ccgtccatga agccatgaga ctagttgcaa
      541 aaccaaggca ggaggtgatg atcacggccg gaccaggcaa agccaggtca agcctttgaa
      601 gaagcgattt tttcttctca acaagggtcg aacttgtctc aatcaccagg ggcgttgtcg
      661 gtgtaagctc ggccagatct gaaagcagaa ctgcgtcaag cccggttcca cggctgagct
      721 cgagcagctc ctgagacaat cgaccttcgc cgataatggc gagactattt cttgtcatag
      781 acgtaaaaac cccgacccgt tttccttccg agattccctc ccatgaccat tttatgctga
      841 atcgggcttg ggcgaaaccg cggatcttca aaaaacgatc ggtagagcga ctccgttgct
      901 gcaaagttga cgtcgtgtcc gatcaaatcc atcagctcaa aaggacccat gcggaaattg
      961 cccgccagtc tcataatgcg atcgatggtt tcaatagtcg cttccccgtc acccaatatt
     1021 cgcagcgcct cgttataaaa gggacgggcc acccggttaa cgataaagcc cggagtatct
     1081 tttgcgcggg ccggagtttt gcccatctgg cgcattagct cggttgccct ggcgatcact
     1141 tccaaagttg tgcgatcgcc ctggatgacc tccaccaatg ccatcagcgg cggaggattg
     1201 aagaagtgca gaccgagaac tcgctccggg tgctgagtgt ccgccgcgat agccgtaacg
     1261 gaaaaggaag acgtgttggt ggccagcagg gtttcgggac cgcatatccc atcgagttgc
     1321 ttgaaaatat cacgcttgag ctccaacttt tccggaacgg cttcgatgat cagagtactt
     1381 tcttcgaagg tttcaagttt ggttgtgctg cgaaggcggt cgagaatctg cttctcgtca
     1441 tcggggctga tcttcccacg ttggcgactt ccttgcagga agtcgcggat ccgacccaag
     1501 cccgcctcga tgaattcctg cttgatgtcg tacaggagag tttcaaaacc cgcttgcgca
     1561 gccacttggg cgatgcccgc gcccatggtg ccggcgccca aaacaccaat tttcactgat
     1621 tgctcagcca tatgcttgaa ctacaactcg tctcgcgtct ggggtttcgg gtttaggaca
     1681 ctagacgcca gacacaaaac ccgagacttg ccattcctat ttccccgtga acaccggagg
     1741 gcgcttttcc agaaacgcgc gcactccctc gtcgtaatcg gtactccgcc cggcaatttc
     1801 ctgcaaatgg gattcgtatt ccaggtccgc ttcaatatcg ggtagaatcg catggttcaa
     1861 ggcccgcttg atcagaccaa tgccccgcgg cgcgccggcg actcttgcgg caatctcctt
     1921 ggtcgacttg gcaaactccg ctgccgagaa gactttggcg acgagcccga tacgctgggc
     1981 ctcatgcgca tctatcgtct cgccgagaag aagcagctcc atggctttac tgaggccgac
     2041 gaggcgcggc aagataaaac taccgccgga gtccggcgcc aaaccgacgc ggacaaagga
     2101 ctgaatgaac ttcgcctctt cggaagcgac ccgcaggtca caggccagag ccaaattgca
     2161 acctgctccg gcggcaacgc cgttaacggc gccgatgaca atcttttcca tctgccggat
     2221 ctggcgaatc atcggattat acttctcgcg taacgaagcc ccgagggatc ctttctgctc
     2281 cggcgtccgg cctttgagat cctgtccggc gcagaaggct cgtcccgcgc ccgtgatgac
     2341 aaagcaacgg atttctttat cgcgctcacc ctctttcaat acttccaaca cttccttgtt
     2401 catctgggga acaaaggcat taagcgcttg cgggcgattg agcgtgatcg tggcgacgcc
     2461 gttttctttc tcataaagga tggtttcgta tggcacggcg ctttactttc ttagctgcct
     2521 ttgaactccg gcttacgctt ttcgagaaag gctttcatcc cttctttgcg gtcctctgag
     2581 gcgaacaaca gataaaatga cttgcgttcg aactccaagc cttcgtcgag gttggcgttt
     2641 gcggacttga gtaccgcctc cttggccgcc ctcacggcga gaaccggttt cgcggcgatc
     2701 ttcttggcta ggttctttgc ttcgtcgagc agcagctcaa cggggacaac gcggttcacc
     2761 aagccaaagc gctcggcatc ggttgcgctg attgtttctc ccgccaatac tatttccatg
     2821 gcccgatatt tccccagggt tcgggttagc ctttgggttc cgccgctacc cggaatgatc
     2881 ccgagattaa cctccggttg gccgaagcgc gctgtctccg aggcgacaac gatgtcacaa
     2941 ctcatagcga gttcgcagcc gcctcctaac gcgaagccgc tcacggcagc gatgaccggc
     3001 ttcgtgattc ggttgattcg atcgcgatag gccaatcgct cttggattct cgcgtcgaag
     3061 gggccccgat cggccatttc tttgatatcg gcgccggcag caaacacctt ctctccgccg
     3121 gtgacgatca taactcgaac ctcgtcgtca tgatcgagct cttgcatagc aagggacaga
     3181 tccttgacta ggtcgtagga gagggcattc agttgctggg gccggttgag agtgagaacc
     3241 gcaatcgagg cgtctttttc aacgatgacg ttatcaaatt gcatacaatc cagacttttt
     3301 acaaatacac catgaaaagg ggggattcaa ggtagagtga gaacgcgcat gccgcaaaaa
     3361 gcgttaaaaa tcattggcct acgaagtcct tccccagtag aatgactccg tgacttcaag
     3421 ttgacacact cccagctatt tgcaactata aggcctcatc catgtccaac cggctcgaac
     3481 ccacttcccg taactacgac gaaaaactcc agcgtatatt aaaaacctcg gcaaaaatct
     3541 ttgctgaaaa gggatttcac cgcacgtcgg tacgggacat ctcgcgcgcc acgaaaatga
     3601 gtctggcagg cctttattat tacttcacga ccaaggaaga actgctctat cttattcaag
     3661 aacgctgttt tgtcacgctg ctgcaacgtt gggaacgggc cgcggtcccg gagatagacg
     3721 cgcgtactcg cattcggttt tttgccgaaa accacctgag ctttttctgc cataatatgc
     3781 acgagatgaa ggtgatggcg cacgaagacg aatccctcac gggcgagttc caggtcaaaa
     3841 tcttagtgct caagcggcgc tacgtaaaag tcatcatgga tctcattcgg gaacttcaag
     3901 atcaagaagc tgggaaaaag gttgacctgc gcgtcgcgac ctttgcactg ttcgggatga
     3961 tgaactggat ctatacctgg tatcagccga aacgtgactt gccgcttccc caactgatgg
     4021 agcagatgct gcgcatctac tttttcggtg tgctgcaagg agctgcagtt gaggccccct
     4081 ggttcaattc aatttccgct gcctcgagca aggaaacgtt ttctctttgg cataatcatc
     4141 agtagacaag gtgaagcgag aaccgggatg agtttgctcg aagtccgtta tgattcgcgt
     4201 tggcgataag gcaccgaatt tcaccttgaa gggcattttc cgcggaacgg tctcggattt
     4261 cgcgcttcaa tctttttcgg ggaaatggtt gatcctattt ttctatcccg cggattttac
     4321 gttcatctgc cctactgaag tggctgggtt cagcaagatg gcacaccagt ttcgtgccga
     4381 ggatgccgaa atcctcggag tcagcgttga ttcgctggaa agtcatcgaa aatgggcgga
     4441 agaattgggc ggcgtgaact atccacttct cagcgacgac ggaaagacgg tcagtcgtct
     4501 ttacggcgtg ttggatgaaa aggaaggcgt gtgtttgaga gggacgttta ttattaatcc
     4561 tacaggcgag ataacctttc tagttataag tcacaccaat gtgggccgca gcgtcgaaga
     4621 aacgctgcgc gtcacgaagg cgctgcgcag cgagcgactt tgcccagccg attggcatcc
     4681 cggtgaaccc acgggcgatc taaaccttaa atattaagcg ctatgg
//
LOCUS       DASSWZ010000338         9141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1414041, whole genome shotgun sequence.
ACCESSION   DASSWZ010000338 DASSWZ010000000
VERSION     DASSWZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414041"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /gene="yidD"
                     /locus_tag="VFN58_08090"
     CDS             complement(<1..185)
                     /gene="yidD"
                     /locus_tag="VFN58_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET8917987.1"
                     /translation="MHRVCRNIIPVILSAYHKLISPFLPRSCRFFPSCSVYASEAIQK
                     HGILRGLSLGLKRVSRC"
     gene            complement(185..547)
                     /gene="rnpA"
                     /locus_tag="VFN58_08095"
     CDS             complement(185..547)
                     /gene="rnpA"
                     /locus_tag="VFN58_08095"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET8917988.1"
                     /translation="MLSGGKANFPKQARLRKRPEFLSLSRWGKKIYSPHFLIITKPNE
                     RGEARLGVTVSSKVGNAVVRNRVKRLLRECFRRHRHQIVPNQDVLIIAKKDAVSLSWH
                     EVESEIRDILIENRGRRK"
     gene            complement(557..718)
                     /gene="rpmH"
                     /locus_tag="VFN58_08100"
     CDS             complement(557..718)
                     /gene="rpmH"
                     /locus_tag="VFN58_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET8917989.1"
                     /translation="MKRTYQPSNVRRKRTHGFLIRMETPGGRKVLKRRRAKGRKRLTV
                     RVPPKRART"
     gene            complement(792..1799)
                     /locus_tag="VFN58_08105"
     CDS             complement(792..1799)
                     /locus_tag="VFN58_08105"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET8917990.1"
                     /translation="MLKPIGIIGAGGWGIALAKLLADKGEQTTLWCHGAESYRELQEK
                     RESRTYLAGIVLPSSIKFTRSIEAAVTDKSLVICVVPSHTVRGVIASAASHVSSEATV
                     LCGTKGLEEESLKTMGEVLVEIFGDPRKQRHAFLSGPTFAIEVARGLPAAVTVAARLE
                     EIARDVQETMSTQNFRVYTSTDIVGVQMGGVIKNIIAIAAGISDGLNLGHNARAALIT
                     RGLAEMTRLAVRMGADSMTLAGLPGLGDLVLTCAGDLSRNRKVGIQIAQGTSLQEIIQ
                     GTQMVAEGIRNTRSVYLLSQRLGVEMPIVGQMYKVIYEGKKPAEAVRDLMQRSLKPEL
                     S"
     gene            complement(1893..4340)
                     /gene="gyrA"
                     /locus_tag="VFN58_08110"
     CDS             complement(1893..4340)
                     /gene="gyrA"
                     /locus_tag="VFN58_08110"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET8917991.1"
                     /translation="MEATLKQQKIPVYIEDEMRQSFMDYAMSVIISRALPDVRDGLKP
                     VHRRVLYAMYDMGNSWDKPYKKSARLVGDVIGKYHPHGDAAVYDTIVRLVQAFSMRYP
                     LIDGQGNFGSIDPDPPAAMRYTEIRMARLAEELLADIEKETVDFIPNYDDSLKEPAVL
                     PSRIPNLLVNGASGIAVGMATNIPPHNLGEVIDGLIALVENPDLTIKQLMKFIPGPDF
                     PTGGFIHGKEPIAQAYSEGKGIIQMRGKAFTETVKRTGKEQIVISEIPFMVNKNRIIE
                     QIAGLVQEKKVEGISDLRDESDREGMRIVVELRRDAIADIVLNQLYKHTALQESFGVN
                     MLAIADGRPKVLNLKEALKAFLDHRKEVVTRRTAYDLRKAEERLHILEGLKIALDHLD
                     AVITLIRRSQDPKAAKDGLMQNFGLSELQAQAILDMRLQRLTGLERDKILEEHKETVE
                     LIAKLRAILANEKEIYRIIVEELKEIKEKYGDERRTQIVAQSEEISIEDLIVDEDMAV
                     TISHEGYIKRNPVTLYRAQRRGGKGKIGATTKEEDFVEYLFIASMHSYILFFTTIGKV
                     YWIKVHELPQASRAARGKPIVNLLNLEPGEKVSAFLTVREFQEGRYVVFATKKGLVKK
                     TELMSYANPRSNGIRAIALEDKDEVIGVRLTDGQQEIILSTADGQSIRFKEEQVRSTG
                     RGTYGVVGMRLDDGDLVVSMEILSLGCDVLTVAENGFGKRTETNEYRLQSRGGKGIIT
                     MKTTDKTGRVVGAQQVTEDDQLMLVTNTGKIIRLRIKDIRVIGRNTQGVRLIDLEEGE
                     RVVSLARLAEKEDEEEE"
     gene            complement(4431..6860)
                     /gene="gyrB"
                     /locus_tag="VFN58_08115"
     CDS             complement(4431..6860)
                     /gene="gyrB"
                     /locus_tag="VFN58_08115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET8917992.1"
                     /translation="MTEPNTPQASDYRADNIKVLEGLEAVRKRPGMYIGDTSERGLHH
                     LVYEVVDNSIDEALAGYCDKIDVTIHIDNSVTVVDNGRGIPVDIHKTEKVSAAEVVLT
                     KLHAGGKFDKASYKVSGGLHGVGVSVVNALAETLEVEIKRDGKVYQQSYKRGEPQGSL
                     KEVGTSKERGTRVTFKPDTKIFETTEFSFDILSQRLRELAFLNRGVKITIEDERSQKK
                     HELFYKGGIVSFVEHLNRAKSAIHSKVIYLQGEKEGVDVEIAMQWNDGYVENVFSFAN
                     NINTLEGGTHLIGFKSALTRTINSYAIDSGQLKKDDENLQGEDVREGLTAVISVKVPE
                     PQFEGQTKTKLGNSEVKGIVETLLNERLANYLAEHPTDAKKIMMKGVEAARVREATRK
                     AKDLARRKGALDSGSLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRDRRNQAILP
                     LKGKILNVEKARFDKMLSSQEIRLMITALGTGVKEDYDAGKLRYHSIVIMTDADVDGS
                     HIRTLLLTFFYRQMPDLVEKGHLFIAQPPLYKVKRGKQERYIKDETGLEDYLIEIGTE
                     DIRVRTSSNGNGLAGQPLQAFIKKVLRWEKLMAVAARKRRSRSVLEALLLEPDFSEET
                     LKDEKALEKFQTDLESYINLVAPDEAPLSCSLEEDPEHNCFKLICATRSNGTGLHTVI
                     DRELLTSVEYKELKRLFSELMSPGSPPYSVENGEQKAKLRSFTELADYILQGGKKGQY
                     IQRYKGLGEMNPEQLWETTMNPETRVLLQVRIEDAVEANDIFSTLMGDEVEPRRKFIE
                     EHALTVKNLDI"
     gene            7098..7808
                     /locus_tag="VFN58_08120"
     CDS             7098..7808
                     /locus_tag="VFN58_08120"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET8917993.1"
                     /translation="MKSAFAAFKFLTLASRFDRTQASPQQVGAAIPYFPLVGIFLGLV
                     LVLLNRFLDPRLESEILGSLLIAFLALLTGAIHYQGLQNTFDALSVKKGRREETGGSH
                     AFGVLAIVFVVLLKVRAVEVTGETRSLGLLLTPLFARWSLVIFLYGAASIAEGTARIL
                     ADNVKAWHLALTTVVTLALAAFLVGRTALWIGLCLSLFALLSRSYLRRRNDCIDSDNF
                     GAVVELSETLSFVLFASL"
     gene            7994..8143
                     /locus_tag="VFN58_08125"
     CDS             7994..8143
                     /locus_tag="VFN58_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8917994.1"
                     /translation="MLYGSLPAASMNFILTEKFGQDPNLAASIVVLSTFFSVVTIPLV
                     FWLIL"
     regulatory      8188..8405
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8557..8802
                     /locus_tag="VFN58_08130"
     CDS             8557..8802
                     /locus_tag="VFN58_08130"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HET8917995.1"
                     /translation="MSVLPKNPDPRRCPLCGDLNACGIAAGEKNCWCFTELVPPEILK
                     KIPAEARGIACVCRFCVSSQRALRKALEGMSDLLRRR"
     regulatory      8900..9101
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1947 a         2467 c         2374 g         2353 t
ORIGIN      
        1 tggcagcgac ttacccgttt gagcccgagt gataatcctc tcagaatccc gtgtttttga
       61 atagcctcag aagcatagac ggagcagctt ggaaaaaaac ggcacgagcg aggcaagaaa
      121 ggcgatataa gcttgtggta cgccgacaag ataacgggaa taatgtttct acaaactcga
      181 tgcactattt ccggcgccct ctgttttcga tgagaatatc tcgaatttca ctctcgacct
      241 catgccagga aaggcttacc gcatccttct tggcaataat gagcacatcc tggttgggga
      301 caatttggtg gcgatgcctg cggaaacatt ctctgagaag ccgctttact cggttgcgga
      361 cgaccgcatt tcctactttc gaactaacgg ttacgccaag ccgggcctct cctcgctcgt
      421 tcggtttggt gataatcaag aaatgggggc tatagatctt tttcccccaa cgcgacaggc
      481 tcagaaactc tggccgtttt ctcagacggg cttgctttgg aaaattcgcc ttgcctcccg
      541 acaacatcct ccgttcttaa gtacgagccc gtttcggcgg cactctaact gttaaccttt
      601 ttcgcccttt ggcccttcgt cttttcaata ccttgcgtcc cccgggagtt tccatccgaa
      661 tgagaaagcc atgggtccgt ttgcggcgga cgttgcttgg ctggtaggtt cttttcatag
      721 aagtagtact cccttgggcc gattagatgg ccaagagttt atttcaatca tagactccag
      781 gtattgtcaa tttaggaaag ttccggcttg agagagcgct gcattaggtc acgcacggct
      841 tcggcgggct ttttgccttc ataaatgacc ttatacattt gccccacaat cggcatttct
      901 actcctagcc gctgagaaag caaatagaca gagcgtgtat tgcggatgcc ttcggccacc
      961 atctgagtcc cctggatgat ctcttgcagg cttgttccct gggctatctg tatacccacc
     1021 tttcgattcc ggctgaggtc gccggcacag gtcagtacca gatcccccaa gcccggaagt
     1081 cccgcgagag tcattgaatc cgcccccatg cgaaccgcca agcgggtcat ctctgcaagc
     1141 ccgcgggtaa ttagagcggc acgcgcatta tgacccaggt ttagaccgtc gctaattccg
     1201 gcggcaatgg cgataatgtt cttaatcacc ccacccatct gcacgccaac gatatccgta
     1261 gaggtataga ccctaaaatt ctgcgtgctc atggtctctt ggacgtctct tgctatttct
     1321 tcgagacgcg cagcgacggt aacggcggcc ggcaagcccc gagcaacttc aatggcaaat
     1381 gtcggaccgg acaagaaggc atgtctttgt ttccgcgggt cgccgaagat ctcaactagg
     1441 acctctccca tagttttcaa actttcttcc tcgagcccct tggtaccgca cagcacggtc
     1501 gcttccgagg acacatgaga cgcagcgctg gcgatgactc cacgcaccgt atgggacggc
     1561 acaacgcaaa taactaagga tttgtcggtg acggcagctt cgatggagcg agtaaattta
     1621 atactcgacg gcaggacgat acccgccaga taggtgcgac tctctcgctt ctcctgaagc
     1681 tcgcgataac tttctgcccc gtggcaccag agcgtggttt gttctccctt atcagcgagt
     1741 agtttcgcca gggcaatacc ccaaccgcct gccccaataa ttccgatcgg cttcagcata
     1801 ctggtgaaaa ggtcacggca gcgaaagaaa aatcaagctc ggagcgaact cgtcaaacga
     1861 aatcgtgagg gacacggtga atcgacggaa ggctactctt cttcctcgtc ctctttctcg
     1921 gccaggcgtg ccaacgatac cacccgctcg ccttcctcga ggtcgatcag tcgcacgcct
     1981 tgcgtgttgc ggccgatgac ccggatatct ttgatgcgca gacgtatgat cttgcccgtg
     2041 ttggtgacca gcatgagctg atcatcctcg gtcacctgtt gcgcgcccac gacacggccg
     2101 gtcttatcgg tcgttttcat cgtgatgata cccttgccgc cgcgggattg cagtcgatac
     2161 tcattggtct ccgtgcgctt tccgaagccg ttctcggcca ccgtgaggac gtcgcagccg
     2221 aggctcagta tttccatact gaccaccaaa tccccatcat cgagccgcat accgacgacc
     2281 ccgtaagttc cacgaccggt tgaccggacc tgttcttcct tgaagcggat ggattgtcca
     2341 tcggcggtgg aaaggatgat ttcctgctgg ccatccgtga gacggacacc aatgacctcg
     2401 tctttgtctt ccagggcgat ggcgcgaatc ccgttagagc gcggattcgc ataggacatc
     2461 agctcggttt tctttacgag gccctttttt gtggcaaaga caacgtaacg cccttcttgg
     2521 aactcacgga ccgttaagaa agccgaaact ttctctcccg gttcgagatt gagcagattg
     2581 acgatcggtt tgcctctcgc ggctctgctc gcctgcggca gctcgtgcac cttgatccag
     2641 taaaccttgc cgatggtcgt gaagaaaaga atgtacgagt gcatggaagc gatgaagagg
     2701 tactcgacaa aatcttcttc tttggtggtc gcaccgatct ttcccttgcc gccgcgtcgc
     2761 tgcgcgcggt agagagtgac cggattacgt ttgatgtaac cttcgtggga aatggtgacc
     2821 gccatgtctt cgtcgacgat caagtcctcg atggagatct cctcggattg ggcgacaatc
     2881 tgggttcttc gctcgtcgcc gtacttttcc ttgatttctt tcagctcttc gacaatgatc
     2941 cgatagatct ccttctcatt tgcgagtatc gctcgaagct tagcaatcag ctccaccgtt
     3001 tccttgtgct cttcaagaat cttgtcgcgc tccaaacccg tcaatctttg cagccgcatg
     3061 tccaggatcg cctgggcttg gagttcggag agcccgaaat tctgcattaa accgtctttt
     3121 gcggctttgg gatcctggga gcggcgaatc agagtaatga cggcatcgag atgatcgagg
     3181 gcgatcttca ggccctcgag gatatggagg cgctcttccg ccttgcgcag gtcgtaggcg
     3241 gtgcgccgtg tcaccacttc tttacggtga tccaagaagg ctttgagcgc ctctttgagg
     3301 ttaagtacct ttgggcggcc gtcggcaata gcgagcatgt tgacgccgaa agattcttgc
     3361 aacgccgtgt gcttgtagag ctggttgagg acgatgtccg ctatggcgtc gcgcctgagt
     3421 tcgacgacaa tgcgcatccc ttcgcggtcc gattcatcgc gaagatcgct gattccttca
     3481 accttcttct cctgtaccag accggcaatc tgctcgatga tccgattttt gttgaccatg
     3541 aagggaattt cactgataac gatctgctct ttgccggtgc gtttgacggt ctcagtgaaa
     3601 gccttgccgc gcatttggat gatgccttta ccttcgctgt aagcttgagc gatgggctcc
     3661 ttgccatgaa tgaaaccgcc ggtcggaaaa tccggccccg ggataaactt catcaattgc
     3721 ttgatcgtaa gatccggatt ttccaccaag gcaatcagtc catcgatcac ttctcccaga
     3781 ttgtgcggcg ggatattggt cgccatgccg accgcgatac cggaggcgcc gttgaccagc
     3841 agattgggaa tgcgcgacgg cagaaccgcc ggttctttga gggagtcgtc gtagttcgga
     3901 atgaaatcga cggtctcttt ttcgatatcc gccaacagct cttcggcgag gcgggccatc
     3961 cggatctcgg tgtagcgcat ggccgcggga ggatcgggat cgatggagcc aaagtttccc
     4021 tggccatcga tgagcggata acgcatcgag aaagcttgca ccaaccggac gatcgtatcg
     4081 taaaccgcgg catcgccatg gggatgatat ttaccgatga catcaccgac gagacgcgcc
     4141 gatttcttat agggcttatc ccagctattc cccatgtcgt acatggcgta gagcacccgg
     4201 cggtggaccg gtttgagccc atcgcgcacg tcaggcagag cgcggctgat gatcacgctc
     4261 atggcgtagt ccataaagga ctggcgcatc tcgtcttcga tatagacggg tatcttttgt
     4321 tgtttcagcg tcgcttccat agtcttggga gttcaagcag ttccaatggt tccagccgtt
     4381 tcattcgtct ggaactcatg gaactaatgg aacgtctgga actttgatcg ttagatgtcc
     4441 agattcttga ccgtcagcgc atgctcttcg atgaacttgc gccggggttc cacttcatcg
     4501 cccatcaacg tggagaaaat atcgttggct tcgacagcgt cttcaatacg gacttgcaaa
     4561 agcacgcggg tctcggggtt catggtcgtc tcccatagct gttccgggtt catttcccct
     4621 agacctttgt aacgctggat gtattggccc ttcttgccgc cttgaagaat atagtccgcg
     4681 agctccgtaa aagagcgcag cttggctttt tgttcgccat tctctaccga gtaaggcggc
     4741 gaccccgggc tcatgagttc actgaaaagt cgcttgagct ctttgtactc gacgcttgtc
     4801 agcagttcgc gatcaataac ggtatgcagc ccggtgccgt tggagcgcgt ggcacaaatg
     4861 agcttgaagc aattatgttc cggatcttct tccagcgaac aggaaagcgg cgcttcgtcc
     4921 ggcgcgacca ggttgatgta cgattcgaga tcggtttgga atttctcgag cgccttttca
     4981 tccttgagag tctcttcgct gaaatccggt tccagcaaca gagcttcgag gaccgaccgg
     5041 cttcgccttt ttcgcgccgc caccgccatc agcttctccc aacgcagcac cttctttata
     5101 aaggcttgca gcggttgtcc agcgagaccg ttaccattac tcgacgtccg cactcggata
     5161 tcctcggtgc cgatctcgat taaataatct tccagcccgg tttcatcttt gatgtagcgt
     5221 tcctgtttcc cccgtttcac cttgtacaaa ggcggctggg cgataaagag atgccctttt
     5281 tccactaaat cgggcatctg ccggtaaaag aaggtgagca acagcgttcg gatatgggag
     5341 ccatccacat ccgcgtccgt catgatcacg atggaatgat accggagctt gcccgcgtca
     5401 taatcttcct tgaccccggt gcccagggcc gtgatcatca ggcgaatctc ttgggaggaa
     5461 agcattttgt cgaaacgggc cttttccacg ttgagaatct tgcccttgag cgggagaatc
     5521 gcctgattgc gccggtctct cccttgtttg gctgatccac cggcggaatc cccctccacc
     5581 aagtagagct cgctcaaagc ggggtctcgc tcctgacaat cggcaagttt tcccggtaaa
     5641 gaaccagaat ccaacgcgcc tttgcgccgg gcgagatcct tggctttgcg tgtcgcctct
     5701 cttacccgcg ccgcttcaac gcctttcatc atgatttttt tggcgtcagt tggatgttcg
     5761 gcgagatagt tggcgagcct ttcgttcaat agagtctcaa cgatgccttt cacctcgctg
     5821 ttacccagct tagtctttgt ctggccttca aattgaggtt ccggaacctt aacgctgatg
     5881 acagcggtca agccttcgcg cacatcttct ccctgaagat tctcgtcgtc tttcttaagc
     5941 tggccgctgt ctatcgcgta gctgttgatg gtgcgtgtga gcgcggactt aaaaccgatc
     6001 aggtgggtac caccctccag ggtattgatg ttattagcga aagaaaagac gttctccaca
     6061 tagccgtcgt tccactgcat ggcgatttcg acatcgacgc cctctttttc accttgtaag
     6121 tagatcactt tcgagtgaat cgcagatttg gcgcggttga gatgctcgac aaaagaaaca
     6181 atgccgccct tatagaacag ctcgtgtttt ttctggctcc tctcgtcctc gatggtgatt
     6241 ttcactcctc gattcaggaa cgccagctcg cgcaatcgtt gggagagaat gtcgaagctg
     6301 aactcggtag tttcgaagat cttggtatcg ggcttaaagg taactcgtgt cccgcgctcc
     6361 ttggatgtgc cgacttcctt caaacttccc tgaggctcgc cccgcttgta gctttgctga
     6421 tagactttgc cgtcgcgctt gatctcgact tccaaggttt ctgccaaagc gttaacgacg
     6481 gagactccca ctccgtgaag accgccggaa actttgtaag aagctttatc gaacttgccc
     6541 cccgcgtgga gctttgtaag cacaacctcg gcggcggaga ctttttccgt cttatgaatg
     6601 tccacgggaa taccgcggcc attgtcgact actgtgacgc tattatcgat gtgaatcgtt
     6661 acgtcaatct tgtcgcaata accggccaaa gcctcgtcaa tggagttatc cacaacttca
     6721 tatacgaggt gatgaagtcc ccgttcactg gtgtcaccga tgtacattcc tgggcgcttt
     6781 cggaccgctt ccagcccttc taaaactttt atgttatccg ctctatagtc cgatgcttgg
     6841 ggtgtatttg gttcggtcat ttttaccaac tataactgcg attttacgtg aaaaattccg
     6901 gggttagcct ggtttttgac taatacaaat attaccggaa aaacggccga aagtaaagcg
     6961 aaaaattgtc cataacatat tgatttttcg gataaaatat tgccgttgta aaaacccggg
     7021 aggaccgccc cgcgagtgga cggttaaaag tgtcatccgt gttgacagcg acagaagtct
     7081 gagagaaaag gagggccatg aaaagtgcgt ttgctgcgtt caaattcctc acccttgcta
     7141 gccgattcga tcggactcag gcaagccctc agcaagttgg cgcggccata ccttattttc
     7201 cgttggtagg aatttttttg gggttagtgc tggttctgct gaatcgcttt ttggatccac
     7261 gtcttgagtc ggaaattcta ggttcccttc tcatcgcgtt cctcgcgctc ttaaccggag
     7321 cgattcacta ccagggattg caaaatacct ttgatgcatt gtcagtaaaa aaaggccgta
     7381 gagaagagac aggaggcagc cacgcgtttg gagtgctagc catagtgttt gtagtcttgc
     7441 tcaaggttcg ggccgtcgaa gtgactggag aaacgcgcag cctcggtctt ctgttgacac
     7501 ctttgttcgc gcgttggtca ctggtgattt tcctttatgg cgcggcctcc atcgctgaag
     7561 gcacagcacg gatcctcgcc gacaacgtca aggcatggca cctcgcttta acaacagtcg
     7621 tgaccctggc actagccgct ttcctggtag gacgaacagc actctggatt ggattatgtt
     7681 tgtctctttt tgcgttgctt agcaggagct atctccgaag gcgaaatgac tgcatcgatt
     7741 ctgacaactt tggagcggtc gtcgagttga gtgaaacctt gagcttcgtt ctcttcgcat
     7801 ccttgtagcg caacactgaa ctcgccgccg cccccatgga gatcataggt taccgcctgc
     7861 gtgactttcg gtctttgcaa tggggccaat gctttcggcg gtgcactgct gcgaatcctt
     7921 ggtggattcg ctgccgcgta catcgcggtg aaactatatt agtcggcgcc gacggcatca
     7981 accggcaagt ttactgcttt acggttccct acccgcagct tctatgaact tcattctcac
     8041 agaaaagttt ggtcaggatc cgaatctcgc ggcgtccatc gttgttctca gcactttttt
     8101 ttccgtggta acgatcccgt tggtgttttg gctgatcctt tagcgagatc ccatatttag
     8161 tgttgacaac taattcagca tactataaac ggatttgcag ctgtcggtac gcttgctctt
     8221 cagagagcgt ggaaacaggg aacccggtga gaatctggga ctgccccgca gcggtaagta
     8281 ggaacgaaag tcgtcataag cactggctct cccatgagct gggaagcgac ggcgagtagg
     8341 aagatagcct tccaaagcta aagcgcctac gagtccgaag acctgccgat ggtccgtgta
     8401 accgcacgga aaatgacccg ggcctccgag gggagagaag cgggaattag tgtatcgtcc
     8461 tttcatcttt gttctccctc aagaaatgtc cgagtcttgc ccgagagggc gtaattcttc
     8521 tgcctctcgg tcgtggcaaa tgagagaggg gaacatatga gcgtgcttcc gaagaatccc
     8581 gatcctcgcc gctgtccgtt gtgtggcgat ctcaacgcat gcggcatcgc ggccggcgag
     8641 aagaactgtt ggtgcttcac tgagttggtg ccgcccgaaa tcctcaagaa gattcccgct
     8701 gaggcacgcg gaatcgcatg tgtatgccga ttctgtgtat cgagtcagcg agcgttaaga
     8761 aaagctttgg aggggatgtc cgatttgctc cgtcgacgct gatgatgtta gtgaggcagc
     8821 ctttaaaaat gcccaggaaa ccatctcttg aaggcgatag cgtgtcagag aatatgttct
     8881 aaaccttgac aatctttttc agtcattata attcttgcgc tgttttttta tgctttaagg
     8941 cgtcagggaa gaccggtgaa aatccggcgc ggtcccgcca ctgtaatggc gacgaaagcc
     9001 gcaacgcaag tcactgtcct gagctgagtc gaaggatggg aaggcgcggc gagtagatta
     9061 aagcctaagc caggaaacct gcccagagca ttttcagccc gtctctccgg aagagagaga
     9121 aaggtttttt caatcttttc c
//
LOCUS       DASSWZ010000339         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1372154, whole genome shotgun sequence.
ACCESSION   DASSWZ010000339 DASSWZ010000000
VERSION     DASSWZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372154"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFN58_08135"
     CDS             <1..529
                     /locus_tag="VFN58_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET8917996.1"
                     /translation="SMASPKGMDFRISKLGHIVLQVSDLERSTAFYTQVLGFKVSDVY
                     PEEMMPGGMVFLRCNTDHHCLALVGAGDGKNANRELHHLAFEVSTLAEVIRARDRLRA
                     HGAKIDFEGRRRAGCQIAVEFRDPDNHSLEIYWGVDQVGSDGRVRPSSEWHGVKSLDE
                     AIANPVPGQDTHLPR"
     gene            526..1488
                     /locus_tag="VFN58_08140"
     CDS             526..1488
                     /locus_tag="VFN58_08140"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HET8917997.1"
                     /translation="MNSSPESLEDLIHASSRALVIGVGGGGDVVGALAAARFLEFCGL
                     AFVLGGLSWERFVFDPIPGPRMLSEVENVRVLHPRVWMANPNSQTKTGVFFAESRMAA
                     AHGKEVLLVDINGGADGVVQGLEVAIKELRTDLLLGLDVGGDSLAQGNEPGLRSPLAD
                     SVMLAAYAELERRGHRALWGVFGYGSDGELTVDEMELALSKLAAAGAWLGTTSLTPRI
                     AAELAEVIKTVSTEASAIPVQCFHGGWGEGKIRSDQRTVKLTPLTALIFFMSPTKLFE
                     TLSRPAQAVSKSGSLEAANDALHALGIRTELDLERERSTGAKNS"
     gene            1706..2950
                     /locus_tag="VFN58_08145"
     CDS             1706..2950
                     /locus_tag="VFN58_08145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET8917998.1"
                     /translation="MFRDLHHDAKLLMISRGIRAFAFSYLGVVFAIYLDRLGYSTVVI
                     GMIFSVAYISSALLTAVWGYLSDRLGRRRILMLLAALTIVSNVIYIFFSSLFFILLAV
                     VVASVGSGGSGGGGSGGGPFNPVEEALLAEKCSPENRNQVFSINSFVGSVMGSLGALG
                     SGLPQYLEESWGWEAVSSYKPLFALTILFSIVLFFVYSSISEQHQPRKVEKKISRKTG
                     VFVTKMSILGMVDNFGAGMAGSLVSYWFFLRFGVELKALGVLFFLSYFLAALSFLAAP
                     VVARYLGVVRTMAFSHGLASLIYLVIPFAPTFFLAGLLLTIRSFFAYMDNPLRASFTM
                     AMVKSEERGSAAGVTGLARVVPFGISPTISTYLMQSVSLTLPLFIGGGLQLANDIAFY
                     LMFRHVRPPEELKGPVAQGSSA"
     gene            2947..>3298
                     /locus_tag="VFN58_08150"
     CDS             2947..>3298
                     /locus_tag="VFN58_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HET8917999.1"
                     /translation="MSYFWRIAEERILDAQRAGAFDDLPGKGKPLQLEDLSWVPEDLR
                     IGYHILKNAHVLPPEAELMKDIHTLEDLLKHVEDENQRRALAKSIQFKMIRLDLLKRR
                     SMSVACVRDYGRKLV"
BASE COUNT          638 a          918 c          936 g          806 t
ORIGIN      
        1 aagtatggcg tccccgaagg gaatggattt tcgtatcagc aagctaggcc acatagtctt
       61 gcaagtgagc gacctggaaa gatccaccgc tttttacacc caggttctgg gctttaaagt
      121 gtccgatgtc taccccgaag agatgatgcc cggcgggatg gtctttctac gctgcaatac
      181 ggaccaccac tgtttggcgt tggtcggcgc cggcgatggg aaaaacgcga accgggaact
      241 gcaccatctc gctttcgagg tctcaaccct ggcagaggtc atccgcgcgc gcgaccgcct
      301 gcgcgcgcat ggggcgaaga tcgacttcga aggccggcgc cgcgccggtt gccagatcgc
      361 cgtggagttt cgcgacccgg ataatcattc cttggaaatt tactggggcg tcgatcaagt
      421 cggcagcgat ggccgggtcc ggccttccag tgaatggcat ggcgtaaaat ccttagacga
      481 agcgattgcc aatccggtgc ccggccaaga tactcacctt cctagatgaa ttcctcgccg
      541 gagtctctcg aagatctcat ccacgcttcg tcgcgcgcgc tggttatcgg tgtcggcggt
      601 ggcggagatg tcgtgggcgc attagccgct gcgcggttct tggagttctg cggattggca
      661 ttcgtcctcg gcggcctctc atgggaacgt ttcgtctttg atccgattcc cgggccgcgc
      721 atgctcagcg aagtcgagaa cgtccgtgtt ctccaccccc gcgtttggat ggcaaacccg
      781 aacagccaga ccaagacggg agtttttttt gccgagtcca ggatggcggc agcccatggc
      841 aaggaagtcc tgctcgtcga catcaacggc ggtgccgacg gggtcgtgca ggggctggaa
      901 gttgcaataa aggaattgag gacggacctc cttctcggtc tcgacgtcgg cggcgactca
      961 ttggcacaag gcaacgagcc gggactgcgt agccccctgg cagattccgt gatgcttgcg
     1021 gcctacgccg aattagaaag acgcgggcac cgcgctctct ggggagtctt cgggtatggc
     1081 agcgatggag aactgactgt ggatgagatg gagttggcgc tgagcaaact ggcagcggcc
     1141 ggcgcttggc tcgggaccac ttcgctgacg ccgcggatcg cagctgaatt ggcagaagtc
     1201 atcaaaaccg tctccaccga ggccagcgcc attccagtcc agtgttttca tggaggttgg
     1261 ggagaaggca agatccgcag cgaccaaaga accgtgaagc tcacaccgct aaccgcgctc
     1321 atttttttta tgtcgccgac aaagctgttc gagacccttt cacggcccgc tcaagccgta
     1381 agcaagagcg gctcattgga agcggccaac gatgcgctgc acgccctcgg catccgaacc
     1441 gagcttgatc tcgaacgcga gagatctacc ggagcgaaga attcgtagca tcacttatgc
     1501 tcgagaatct gcagcgggat gttttattac taaggggttc gcccacgctc gccgcctacc
     1561 tcatgcagtc attttccctc gttgtgccta tcttcattgg cagatttttg cagctctttc
     1621 atgactgtgc tgtttacttc ctctttcgcg acgtgaagcc tcgcgaggaa gaagtggccc
     1681 gagcttagaa acggtccgac ccatcatgtt tcgcgatctt catcacgacg ccaaactact
     1741 gatgatttcg cgcggcattc gcgcttttgc cttttcctat ttgggtgtgg tttttgccat
     1801 ttatctggac cggttgggct attcgaccgt agtcatagga atgatctttt ctgttgccta
     1861 catcagcagc gcgctgctca cggcagtttg gggatatctt tcagaccgcc tcgggcgaag
     1921 acggatcttg atgctgctgg ctgctttaac gatcgtttcg aatgtcattt acatcttttt
     1981 tagcagcctg tttttcatat tgctcgcggt ggtggttgcg agcgttggct cgggcggttc
     2041 cgggggaggc ggatccggcg gcggcccttt caatccggtg gaagaggccc tgctcgccga
     2101 gaaatgcagc cccgaaaaca gaaaccaagt tttttcaatc aactcctttg tcggatcggt
     2161 gatgggctct ctcggcgctc tcggcagcgg tctgcctcaa tacctggagg aaagctgggg
     2221 ctgggaagcc gtctcgtcct acaagccttt gtttgcgttg accatcctgt tcagcatcgt
     2281 tctgtttttc gtttacagct cgatcagcga gcaacaccaa ccccgcaaag ttgaaaaaaa
     2341 gatttcgcga aagaccggcg tttttgtgac caagatgtcg atcttgggga tggtcgataa
     2401 ttttggcgcc gggatggctg gctcgctggt ctcctactgg ttttttttgc gtttcggtgt
     2461 cgaactgaaa gctttaggag tgctcttctt cctgtcttat tttctggctg ccctttcatt
     2521 ccttgccgcg cctgtcgtcg cgcgctatct cggcgtcgta cgcacaatgg cattctccca
     2581 cgggctcgca tctttgatat atctggttat cccttttgct ccgacctttt tcctcgcagg
     2641 gttgctcctg accattcgat ccttctttgc gtacatggac aatcccttac gggcttcctt
     2701 caccatggcc atggttaaat ccgaagagcg cggctccgct gccggagtca cgggacttgc
     2761 ccgggttgtc cccttcggca tcagcccgac gatctcgacc tacctgatgc agagtgtgtc
     2821 tttgacactt ccattattca tcggcggcgg gttacaattg gctaacgaca ttgcctttta
     2881 tttgatgttt cgtcacgtga gacctccgga agagttgaag ggacctgtcg cgcaagggag
     2941 ttcggcttga gttacttttg gcgcatcgcg gaagagagaa tcctcgacgc ccaacgggcg
     3001 ggggctttcg atgacctgcc cggcaaaggg aaacctctgc agctcgaaga cttgagctgg
     3061 gtgccggaag acttgcgcat cggctatcac atcctcaaga acgcccatgt cctgccgcct
     3121 gaggctgagc tcatgaagga cattcacacc ctcgaagatc ttttgaaaca tgtcgaagac
     3181 gaaaaccagc gacgggctct cgcaaagagc atccagttca aaatgatccg cctcgatctt
     3241 ttgaagcgcc gctccatgtc ggtggcctgc gtccgggact atggccgaaa attagtcg
//
LOCUS       DASSWZ010000340         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_338917, whole genome shotgun sequence.
ACCESSION   DASSWZ010000340 DASSWZ010000000
VERSION     DASSWZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338917"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFN58_08155"
     CDS             <1..526
                     /locus_tag="VFN58_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET8918000.1"
                     /translation="QPRRDIGIRTIFNLLGPLTSPAMATHQLVGLYSGELVGTIAHVL
                     KNLDSARAMVVHGVEGLDEISLCGPTQVAELRNGQVKEYVIEPEALGLTRCRLDDLRG
                     GTAAESAEVVRAVLQGKKGATRDVVLLNSGAALYVSGSAGSIDDGLRLAAESIDSGKA
                     QQKLEGLVRMTTAA"
     gene            534..1313
                     /gene="trpC"
                     /locus_tag="VFN58_08160"
     CDS             534..1313
                     /gene="trpC"
                     /locus_tag="VFN58_08160"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET8918001.1"
                     /translation="MAEFLATIVEHVKNQVEQRRREMPASALRERPLFQTPTRGFAKS
                     LAGNSRRIIAEVKRASPSKGLIREICDPVAIAKDYASHGASAISVLTEERFFQGSLSY
                     LEQIRSTVNVPLLRKDFIVDPYQLTEARSYGADAVLFIAALLDPSLLKELREQATALS
                     LDSLVEVHTEEELNAAVKAGAQLLGINNRDLKTFEVNLATTEKLAPLVPVGMPAVCES
                     GIDSVEQIRRVEKLGIHIFLIGESLMRASQPGKRLRELLER"
     gene            1346..1972
                     /locus_tag="VFN58_08165"
     CDS             1346..1972
                     /locus_tag="VFN58_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET8918002.1"
                     /translation="MIKIKVCGITNLADAEKSLDFGADALGFNFYPPSPRRMAPEGAR
                     AILQRLPEGCCNVAVFVNEPEAKVREIVAQGELANGRQAYSALQFHGEESQEYCRGWS
                     MKVIKAFRVREKKSLEGMELFPADFYLLDSWSSGYGGSGAAFSWEWLEGVKTEKLILS
                     GGLTIENVVEAIRRIHPYGVDVCSSVEARPGIKDHAKLKEFILAAKGA"
     gene            1953..3149
                     /gene="trpB"
                     /locus_tag="VFN58_08170"
     CDS             1953..3149
                     /gene="trpB"
                     /locus_tag="VFN58_08170"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET8918003.1"
                     /translation="MLPKVPDRRGHFGPYGGRYVAETLMPALTELERAYAQARRDAGF
                     RKDFQRSLHEYAGRATPLYFAERLTEKLGGAKIYLKREDLCHTGAHKINNTLGQILLA
                     RRMGKKRIIAETGAGQHGVATATVAARFGLECEIFMGKEDVARQSLNVFRMKLLGAKV
                     RVVEAGSQTLKDAMNEALRDWVTYVRNTYYLIGSVAGPHPYPMMVRDFQSVIGKEARK
                     QFLKAEGRLPDYLVACVGGGSNSMGLFYPFVRDSKVKMLGVEAAGRGLRTGRHAASIL
                     GGEIGVLHGSKSYVLQDERGQIRQTHSIAAGLDYPGVGPELSYLRDRGRVQFASATDS
                     EAIEAIKFLAEVEGIIPALESAHAIAAVMGLAPRLAKHQTVIIGLSGRGDKDMMTVGD
                     YLGVTL"
     gene            3223..>3378
                     /locus_tag="VFN58_08175"
     CDS             3223..>3378
                     /locus_tag="VFN58_08175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET8918004.1"
                     /translation="MSSIDNKFAELRRKGEAALIPFVTAGDPNLATTLKVLHALEKGG
                     ADCIELGI"
BASE COUNT          757 a          832 c         1052 g          737 t
ORIGIN      
        1 gcagccgcgt cgcgatatcg gtatccgtac catctttaac ctgctgggac ctttgacgag
       61 tcctgcgatg gcgacgcatc agctagtggg tctatacagc ggcgaattgg tcgggaccat
      121 cgctcatgtt ttgaagaacc tcgatagcgc tagggccatg gtcgttcacg gcgttgaagg
      181 gttggacgaa atctccctct gtggtcccac ccaagttgcc gagctgcgca atggacaggt
      241 taaagagtac gtgatcgagc cagaagcgct gggacttacg agatgtcgct tagacgatct
      301 ccgtggcggc accgcggcag agagtgccga ggtagtgcgc gctgtgttac agggaaagaa
      361 aggggctacg cgggacgtgg tgctgctcaa cagtggtgcg gccctctatg tcagtggcag
      421 cgccgggtcg atcgatgatg gcctgcggtt ggcagcggag tcgatcgatt ctggcaaggc
      481 gcagcagaaa ctggaggggc tggtgcggat gacgaccgct gcgtaaacct tccatggctg
      541 aatttctcgc caccatcgtt gagcacgtta agaaccaagt ggagcaacgg cgacgggaga
      601 tgccggcgtc ggctttacgc gagcggccgc tgtttcaaac gcccacccgt ggatttgcca
      661 agagtttagc gggaaactcg cgtcggatca ttgcagaagt caagcgagcc tccccttcca
      721 aagggttgat tcgggaaatt tgcgatccag tagcgatcgc caaggactat gcaagtcacg
      781 gagcaagcgc gatctcggtc ctcacggagg agcgattttt ccaggggagc ctttcatatc
      841 tggagcagat tcgcagcaca gtaaatgtgc cgttgctgcg taaggacttc atcgtcgatc
      901 cctaccagtt gacggaagca cggagctatg gggccgacgc ggtcttgttc atcgcggcct
      961 tgctcgatcc gagtttgctt aaggaattgc gtgagcaggc cacggctctg tcgttggatt
     1021 cactcgtcga agtgcacacc gaagaggaac tcaatgctgc ggtgaaggcc ggggcacagt
     1081 tgctgggcat caataaccga gacctcaaga cttttgaggt gaacttagcc acgacggaga
     1141 aactagcccc gctggtgccc gtcgggatgc cggcggtgtg tgagagcggt atcgatagtg
     1201 tggagcagat tcggcgcgtc gaaaagttgg gcatacacat ctttctgatc ggtgaatcgc
     1261 tcatgcgggc ctcccagcct ggaaaaaggc ttcgcgagct attggagcgg tagcataaaa
     1321 tcttcagcgg ttaaaattca tcaccatgat taagatcaag gtctgcggca tcacgaactt
     1381 ggcggacgcc gagaagtcgc tcgacttcgg tgcggatgct ttaggattta acttctatcc
     1441 cccgagtccc cggcgcatgg ccccagaggg cgcgcgcgcg attctacaga gattgcctga
     1501 agggtgctgt aacgttgcgg tctttgtcaa cgagccggag gcgaaggtgc gcgagattgt
     1561 cgcacagggg gagctcgcca atggacgcca ggcttatagc gctctacaat tccatggtga
     1621 agagagccag gagtattgcc gaggctggtc gatgaaagtc attaaggcgt ttcgagtgag
     1681 agaaaaaaaa tccttggaag gtatggaact atttccggcg gatttttatt tgttggattc
     1741 gtggtcgtcg ggatatggcg gatcaggtgc cgcattttcc tgggaatggc tcgagggagt
     1801 gaagactgaa aaactgattt tgtcgggtgg tctaactatc gaaaacgtcg ttgaggccat
     1861 tcgccggatc catccctatg gtgtcgatgt ctgcagcagc gtggaagcac gtccgggtat
     1921 caaagatcat gcaaagctca aggaattcat tcttgctgcc aaaggtgcct gatcgacgcg
     1981 gtcatttcgg tccctacggc ggccggtatg tggcggagac tcttatgcct gcgctcactg
     2041 agctagagcg agcctatgcg caggcgcgtc gagacgcggg ctttcgcaaa gatttccagc
     2101 gctctcttca tgaatacgcc ggaagagcaa cgcccctcta tttcgccgag cggctgaccg
     2161 agaaactggg cggcgcgaaa atctacctca agcgcgagga tctctgccac accggcgcgc
     2221 acaagatcaa caacaccctt ggccaaatcc tcctagcgcg cagaatgggg aagaagagaa
     2281 ttatcgcgga aacgggcgcg ggacagcatg gagtcgctac ggcgacggtg gctgcgcgat
     2341 ttggtctgga gtgtgagatc ttcatgggca aggaagatgt cgcacggcaa tcgttgaatg
     2401 tatttcgcat gaaactgctt ggcgcgaagg tgagggtcgt agaagcaggc agccagacct
     2461 taaaagacgc aatgaatgag gccctgaggg attgggtcac ctacgtgcgc aatacgtatt
     2521 atttgattgg ctccgtggcc ggtccgcatc cctacccgat gatggttcgt gactttcaat
     2581 cggtgatcgg gaaagaggcg cggaagcaat ttctaaaggc cgaggggcga ttacccgatt
     2641 acctggtcgc ttgtgttggc ggcggcagca attccatggg gcttttttat ccctttgtac
     2701 gcgactccaa ggtaaaaatg ctcggcgttg aagcagccgg aagaggttta cgcacgggcc
     2761 gacatgcggc gtctatcctt ggcggggaga ttggtgtgct ccacggtagc aaaagttacg
     2821 tcctgcagga tgagagagga cagatccgcc aaacgcattc gattgccgct ggcctggact
     2881 atcccggcgt aggaccggag cttagctacc tacgtgatcg tggtagagta cagtttgctt
     2941 cagccacaga cagtgaagcg attgaagcga tcaaattcct ggcggaggtg gaggggatca
     3001 tcccggcatt ggaaagcgcc cacgcgattg cagccgtcat gggcctcgcg cctcgtttag
     3061 ccaaacacca gaccgtcatc atcggtctct ccggaagggg agataaggat atgatgacag
     3121 ttggggatta tctcggggtg actctctaaa tgtggttcaa gtttaagatt tttctttact
     3181 ttgagcccgg aaccttgaat ttttgaagct cctgtccgaa ccatgagcag tatcgacaat
     3241 aaatttgcgg agctgcgaag gaaaggggag gcagcgctga tcccctttgt gaccgctggc
     3301 gatcccaatc ttgcgactac gctgaaggtc cttcacgccc tggaaaaggg cggtgcagac
     3361 tgtatcgagc tgggaatt
//
LOCUS       DASSWZ010000341         6500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1273514, whole genome shotgun sequence.
ACCESSION   DASSWZ010000341 DASSWZ010000000
VERSION     DASSWZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273514"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..1101
                     /locus_tag="VFN58_08180"
     CDS             442..1101
                     /locus_tag="VFN58_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET8918005.1"
                     /translation="MEGFYDYQKCTILYVDDEEQSLKYFTRAFRNKFRILSAADASEG
                     YRLLEQHRDEIALLMTDQRMPGEKGVEFLQRASELHPKAIRILTTAYSDFDVVIEAVN
                     SAAIYKYVTKPWDIPQLETILEQACEFFTQQRERDLLLAAKVPAPEQKTIAGRGVESG
                     DLQTPEQVARYLNVTKFTLYRLVTQKKIPAFKVGNQWRFKQEMIDAWLMAKSGIRKKS
                     D"
     gene            1514..1915
                     /locus_tag="VFN58_08185"
     CDS             1514..1915
                     /locus_tag="VFN58_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET8918006.1"
                     /translation="MANKTKKILIVDNNDAWRDTLGGLIKGLGHEVFEAATGSEAIDR
                     ASSLHPDLIMMDLSLPEMNGDEATVRLKRNASTRNIPVVINTSWSMSCNVGDRVDRAL
                     NAGAEEILYKPYHLSTLRGVLRTYLMTSDSQ"
     gene            2283..3557
                     /locus_tag="VFN58_08190"
     CDS             2283..3557
                     /locus_tag="VFN58_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET8918007.1"
                     /translation="METVSSRKTIEERQLKRLQQMLKAVLPSNEFYRNKFSTHGSCEI
                     SSMETLKTLPFTTKNELVEDQMRHPPFGTDMTFPRERYIRIHQTSGTTGKPMYWLDTE
                     ESWEWWAECWKVILEAAGVSSGDRIFFAFSFGPFIGFWAGWEGARKLGAMAVSGGGQS
                     TSQRLRAIIDYGATVLICTPTYALHLASEARKTGMDLAKESTIRITLHAGEPGASIPS
                     TKKMIEESWGAKCFDHPGATEVGAFGFECQCQPGGVHVNESEFIAEILDPVTGTAVDD
                     GEKGELVVTNLGRTGSPVIRYRTGDLVQPSYQLCPCGRPFLLLQGGVLGRVDDMVIVR
                     GVNVFPGAIENVMREFHEIEEFRVEIFEKEAMKEIRLVVEPRSDQSSGAGLGEQVSQR
                     VRERIGLRPHVELVAPGTLPRFELKAKRFFKL"
     gene            3657..4166
                     /locus_tag="VFN58_08195"
     CDS             3657..4166
                     /locus_tag="VFN58_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET8918008.1"
                     /translation="MRKIKKNGAGGRLTKTLLQLCVNGETQEVATEINKTLLEVLRED
                     MGLTGTKHGCELGECGTCAVLVDGAPVLSCLMLGVEAVNTEIVTVEGMTQDGRPHPLQ
                     KTFADLGAAQCGYCIPGILLTAKALLDKTPQPTRNEIRQALSGNLCRCTGYTKILQAV
                     ELAAKEIVG"
     gene            4240..>6500
                     /locus_tag="VFN58_08200"
     CDS             4240..>6500
                     /locus_tag="VFN58_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET8918009.1"
                     /translation="MTKKKLSVIGKPLRKIDAMAKCAGETVFADDLNLPRMTYAKLLR
                     SPHPHARVLSIDAARALKLDGVFTVITGQDLPEKFGIMPSTQDEETLAIDKVRYAGDP
                     VAAVAAASESLAEKALGYIDVEYEVLKPILTIEEALASTEESERIHTWNRQANIQKAV
                     SFEFGDVDGGFARSDHVFEGTFFYQGNTHLPMEQYCAVGFYGPDDKLTVWSSTQTPHY
                     LHRALAKVLKLPASRIRVVATPVGGGFGGKSDPFSHEIAVAKLSMITRRPVKITLTRE
                     ESFYVHRGRHPVKMLAKTGVKNDGTIQAMQYKTYLDGGGYSSYGLATVYYTGALQTVT
                     YKVPAYKFEGVRVFTNKPACGPKRGHGTPQPRFAQEIQLDEIAEKLGIDPIELRLKQL
                     VEPNSITVNGLRISTTGLRECSERVAERAEWKNKFRKLPFGHGVGFAASSYISGAGLP
                     IYWNDMPHSGVQIKLDRSGGVAVFCGSIDIGQGSDSILAYAVAEEFGIDPADIRVLTA
                     DTDLTPVDLGSYSSRVTVMTGNAAVQACGKLKPMLLEAASKALEVPPDDLEFAGGKIR
                     SASFPERGISFADAVTKAEGKYGTLGATGSYWPPKNPRDFKGSGVGPTPSYSFSTSIV
                     ELHCDSATGEIKIDKVWIAHDCGRAFNPLLVEGQTEGSVYMALGEALMEEQIFRKNGL
                     HKIPSILEYKSPTTLETPEIETILVETNDPEGPYGAKEAGQGPLLPVVPAVANAVYDA
                     IGVRIHEVPITPD"
BASE COUNT         1590 a         1739 c         1776 g         1395 t
ORIGIN      
        1 acccacagtg ggctgtatcc gagcgtcttg ctcgatgccc caagaaaaat cttgggtttc
       61 tcctgcgcca tgactgaaga ggaaaccacg ccgcaaacca caagcgtgac cgcaattcga
      121 aaatggatag ccatgggact ctcctcttgc gtccgattcg ttttcagcaa accgccgtcg
      181 tccaatcctt agtataacag tgcgtggcca tcaagacgaa gattatgatc gtgggatggc
      241 aacgaatccg ggcccgatct agggttattc gaaattggga ccctatcgca tacatcatcg
      301 atttaggata agttaggaat ggctagatgt cgaaaggagt atgggctcgg gcggagggta
      361 gaaagaagca actggcacgc acaaggctaa acgttccgct tttccagagg ccgataaggc
      421 cgatgggcgc agaggtgaga catggaaggt ttttatgact atcagaagtg caccatcctt
      481 tacgtggatg acgaggaaca atcgctcaag tattttactc gagccttcag gaacaagttt
      541 agaattctaa gcgccgccga cgcctcagag ggctaccgtc tcctggaaca acatcgagac
      601 gaaatcgcgc ttctgatgac tgaccaaagg atgcccggtg aaaagggcgt cgaatttcta
      661 cagcgagcca gcgaattaca cccgaaagcg attcgtattt tgactacagc atactccgac
      721 ttcgatgtgg ttatcgaggc ggtcaactcc gctgccattt acaaatacgt gaccaagccc
      781 tgggatattc ctcagcttga gacgatcctt gaacaagctt gtgagttctt tacgcagcaa
      841 cgggagcgcg atcttcttct tgcagccaaa gttccggcgc ccgagcaaaa gacaattgcc
      901 ggccggggtg ttgaatcggg ggatctgcag actccggagc aagtcgcccg ttacttaaat
      961 gtcaccaagt ttactcttta tcgtctggtc acccagaaga aaattcccgc cttcaaagta
     1021 ggcaatcaat ggcgcttcaa gcaagaaatg atagacgcgt ggctcatggc caaatcaggt
     1081 atcaggaaga aatcagatta attcccgcca aggcaagatt tcgtagacta accggccgca
     1141 attcgtagcg atttcgcgaa cctgcttcga gatctttgac tatttcttga gcaaggatgt
     1201 tgtcggggca aaggtttccg attcttcaaa gcctttctca agaagcttcg ctctatgctc
     1261 ctcaattaat tgggattgtg ctacccctgt tcctgggctt tccgagagcg attggaccga
     1321 tgacctgttg tcaagttgtc aaacttacgt aagtaagaat gaccggtaac cggaagaaac
     1381 tactttccgg tatgagtcac acctcaaggg tattcccacg ccgtatatct caggagggtg
     1441 acttcgcggc aacaaatacc aagacagcgg cccttgctct ggctggcatc ccttgtgctt
     1501 ctaccccttt tgtatggcga acaaaaccaa gaaaatattg atcgttgaca ataacgatgc
     1561 ttggcgagac accctgggcg ggcttatcaa agggttaggc cacgaagtct tcgaagccgc
     1621 tacgggttca gaggccatcg acagggcttc cagcctacat cccgatctaa tcatgatgga
     1681 tctaagctta cccgaaatga atggagatga ggcaacggta cgcttaaaga ggaacgcgtc
     1741 gacccgaaac atccccgtgg ttatcaatac ctcttggagc atgtcttgta acgttggaga
     1801 ccgtgtggac cgtgccctga atgcaggggc cgaagagatt ctgtataagc cgtatcactt
     1861 gtcgaccttg cgtggtgttc tgcgcacata tttaatgacc tcggactcgc agtaaagaat
     1921 tcgctaagtt atcgacagga ggaggacagg ttcctatgaa tgagtttttg ttgcttgcgg
     1981 tcgttgtcgt ctacttagcc atcctttacg gaaagcccac catatagaat ctccagcgcc
     2041 agagtcactc cactcccgac cattcggcta ttcacctgac tacgaccgct gcaagagcgg
     2101 tctttttgtt tcagtcgcgg catttcccct aggaaaagtt ttccatctca tcagagccca
     2161 agcgatctca tcatcacttc ttgcagttcc acctcttgca taatagattt cgggcaacga
     2221 gcacccgaat ctcttttcac aacggtgaga aactcagttt gaatgagagt tcaagggcct
     2281 tcatggaaac tgtaagcagc cgcaaaacga tcgaggagcg acaactgaag cgattgcaac
     2341 agatgctcaa ggcggttcta ccgtcgaatg agttttaccg gaataaattt tcaacccatg
     2401 ggtcttgtga gatcagctcc atggaaaccc tcaagaccct accttttacg accaagaacg
     2461 aattggtgga agatcaaatg cgccatcccc cgttcggcac cgacatgacg ttcccacggg
     2521 aacgctacat tcgaattcat cagacctcgg gcaccaccgg aaagccgatg tactggctcg
     2581 acactgaaga atcctgggaa tggtgggcgg aatgttggaa ggtcatcttg gaagcggccg
     2641 gcgttagttc cggggatcgc attttttttg ctttttcgtt tggtccgttt atcggattct
     2701 gggccggctg ggagggtgcg cgaaaactcg gcgccatggc ggtctcgggc ggcggacagt
     2761 ctacttcgca gcggctgagg gcgataatcg actatggcgc gaccgtgctt atctgcaccc
     2821 caacatacgc gctacacttg gcgtccgagg caagaaagac aggcatggat ctcgccaaag
     2881 aatccaccat aaggattacc ctccatgccg gcgagccggg ggcgagcatt ccgagcacaa
     2941 aaaagatgat tgaagagtcc tggggagcaa aatgctttga tcaccctgga gccaccgagg
     3001 taggcgcctt cggattcgag tgccaatgcc aaccgggagg cgtccacgtc aacgaaagtg
     3061 aatttattgc agagatcctg gaccccgtca ccggcacggc cgtcgacgat ggagaaaaag
     3121 gcgagctggt cgtcaccaat ctcggcagga ccggcagccc cgtgattcgt taccgcaccg
     3181 gcgatctagt tcaacccagt taccagctct gcccatgcgg tagaccgttt ttgcttttgc
     3241 aaggtggcgt gttgggccgg gtggatgata tggttattgt ccgtggcgtc aacgtttttc
     3301 ccggcgccat cgaaaacgtc atgcgggaat ttcacgagat cgaagagttt cgcgtggaaa
     3361 tctttgaaaa ggaagcgatg aaggaaatta ggctggtcgt agagccgcgc tcagaccaaa
     3421 gttccggcgc tgggttgggg gaacaagtga gccaacgcgt gcgcgaacgc atcggtcttc
     3481 gccctcacgt tgaactcgtt gcgccgggaa ctttgccgag attcgagctc aaggctaaga
     3541 gattctttaa gttatagggg tggggttcaa acccgcccct acgggatgcc ctgcctgggt
     3601 gtgacgataa gagagtcccg agtgcattat cgaacaaacg actagtcaga acgggcatga
     3661 gaaaaatcaa aaagaacgga gccggtggca ggctgaccaa aacgctattg cagctttgcg
     3721 tcaacggcga aacccaagag gtcgccacgg agattaacaa aactttgttg gaagttttgc
     3781 gcgaggacat gggtctcacc ggcaccaaac atggttgcga gctgggagaa tgcggcactt
     3841 gcgccgtact tgttgacggc gcgccggtgc tttcgtgcct gatgctcggc gttgaagcgg
     3901 tcaataccga gatcgtcaca gtggaaggaa tgacccagga tggccggccc catccgctgc
     3961 aaaagacttt cgccgatctc ggcgcggcac aatgcggtta ttgcattccg gggatactcc
     4021 tcaccgctaa ggctttatta gacaagacac cgcagccgac tcgcaacgag attcgccaag
     4081 ccctgtctgg aaatctctgc cgctgcaccg gctataccaa aattttgcag gctgtcgagc
     4141 tcgccgcgaa ggaaattgtt ggatgacatt gttaactgca gaaaaatttc cctcttttaa
     4201 aaagacgggt taggggagat tttgaacaag ggtgatttaa tgacaaagaa gaagctctcc
     4261 gtgatcggca aaccgctgcg caagatcgat gcgatggcca agtgcgcggg cgagactgtc
     4321 ttcgcggatg atctcaatct tccccgtatg acttatgcga aacttttgcg tagcccccat
     4381 ccccacgcgc gagtgctaag tatcgatgcc gcgcgagcgc tcaagctcga cggcgtgttc
     4441 acggtgatca cgggccagga cttgccggaa aagttcggca tcatgccttc gacccaggac
     4501 gaagaaacgc tcgccattga caaagtgcgt tacgccggcg accccgtggc tgcggtggct
     4561 gcagccagcg aaagtcttgc cgaaaaggca ctaggctata tcgatgtcga atatgaagtt
     4621 ctgaaaccga ttctcaccat cgaagaagcg ctggcttcga ccgaggagag cgagcggatt
     4681 catacgtgga accggcaggc caatattcaa aaggcggtat cctttgaatt cggcgacgtc
     4741 gacggcgggt tcgcgcgctc ggaccatgtt tttgagggca cctttttcta tcaaggtaac
     4801 acccacctgc cgatggagca gtactgcgcg gtgggtttct acggccccga cgataagctc
     4861 acagtttggt cttccaccca gacgccgcac tatcttcatc gtgccctggc caaagttttg
     4921 aaacttccag caagccgcat acgcgtggtc gccactccag tgggaggcgg cttcggcggc
     4981 aagagcgatc ccttttccca cgaaatcgca gtggccaaac tctccatgat cacgcgaaga
     5041 ccggtgaaaa tcacgcttac ccgcgaggag tccttctatg tgcaccgcgg cagacatccg
     5101 gtcaagatgc tagcgaagac cggcgtaaag aacgacggta cgattcaagc gatgcagtat
     5161 aaaacctatc tcgacggcgg cggctacagc agttatggct tggcgacggt ctactacacc
     5221 ggcgcattgc aaacggtcac ctacaaagtg ccggcttata agtttgaagg cgtgcgggtt
     5281 ttcaccaata agccggcctg cgggccaaag cgcggccacg gtacgccgca gccgcgcttt
     5341 gctcaggaaa ttcaactcga tgaaatcgcc gaaaagctcg gcatcgatcc tatcgaactg
     5401 cgcctcaagc aactagtaga gcccaatagc attacggtga acggcttgcg catcagcact
     5461 accggcctgc gcgaatgcag cgagcgcgtc gccgagcgcg ccgagtggaa gaataaattc
     5521 cgcaagcttc ccttcggtca cggcgtcggt ttcgcggcca gctcctacat cagcggcgcg
     5581 ggcttgccca tttactggaa cgacatgccc cactcagggg tacagatcaa gctcgaccgc
     5641 agtggtggcg tggcggtttt ttgcggttca atcgatatcg gccagggttc ggattccatt
     5701 ctggcatacg ctgtcgccga agagttcggc atcgatcccg ccgacataag agtattaacc
     5761 gccgatacgg atttgacgcc ggtggatctg ggcagttact cgagccgcgt caccgtgatg
     5821 accggcaacg ccgcggtgca ggcctgcggc aaactcaagc ccatgctgct cgaagccgca
     5881 agtaaagcgc tggaagtacc gccggacgat ctcgaattcg ccggcggcaa gattcgcagc
     5941 gcaagcttcc ccgaacgggg cataagcttc gctgacgcgg tgaccaaggc cgagggaaaa
     6001 tacggcacgc ttggcgctac cggcagctac tggccgccga agaatccgcg cgattttaaa
     6061 ggctcgggag tcggtcccac accgtcctac agtttttcta cctctatcgt cgagcttcac
     6121 tgcgacagcg ctacgggcga aatcaagatc gacaaagttt ggatcgccca cgattgcggg
     6181 cgcgccttca atccgctgct cgtggaaggg caaaccgaag gcagcgttta tatggctctc
     6241 ggcgaagcgt tgatggagga acaaattttc cgcaaaaatg ggttgcataa aatcccgtcg
     6301 attttggaat acaaaagccc gacgacactg gagacacccg agattgaaac gattctagtc
     6361 gaaaccaacg atcctgaagg accctacggc gccaaagaag ccggccaagg accgctcctg
     6421 ccggtcgtcc ccgcggtggc gaacgcggtt tatgacgcca tcggcgtgcg catccatgaa
     6481 gttccgatca cgccggacaa
//
LOCUS       DASSWZ010000342         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_254436, whole genome shotgun sequence.
ACCESSION   DASSWZ010000342 DASSWZ010000000
VERSION     DASSWZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254436"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFN58_08205"
     CDS             complement(<1..492)
                     /locus_tag="VFN58_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET8918010.1"
                     /translation="MKSMTGYGEASSQIRGTRVTVQVRSLNHRHLDLQLRAPREYLSF
                     EEEIRKVIRQKISRGRLDVFINRSQAKGQSRRLELDEELLGQYLTAVRQAKGKYQLSG
                     DFSLSLLSSIPDLFHVREVEVNAAAERQGVFKILDAALTRLERAREREGRQLKSDMQS
                     QIRH"
     gene            complement(489..1475)
                     /gene="rfaE1"
                     /locus_tag="VFN58_08210"
     CDS             complement(489..1475)
                     /gene="rfaE1"
                     /locus_tag="VFN58_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET8918011.1"
                     /translation="MKRLLDVLNGFHGAHLLVVGDLMLDRFVWGEVERISPEAPVPVL
                     RVTAESSRLGGAANVVNNVRSLGAQVTACGVIGRDLAGKRLLHDLRRAGAATAGIFAD
                     KQVQTIQKSRIIARPRHQQIVRLDRENQNPIGESTLKRLRGFVLARATACDAVIISDY
                     GKGVVHADLLRSIARLVKKNQVICVIDPKKENYEHYRLPTLITPNKDEASAASGIEIQ
                     DEKSLLAAGKKLEQIWQAEAVLITQGPDGMSLFRPRCAVKHFPTEPRDMFDVTGAGDT
                     VVAICGLALACGASFEEAAILANLAAGLVGDEVGTVAVSLDKLKRIVQDGQL"
     gene            complement(1475..2617)
                     /locus_tag="VFN58_08215"
     CDS             complement(1475..2617)
                     /locus_tag="VFN58_08215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET8918012.1"
                     /translation="MTASGHSRLLKILHIDPERHWGGGEAQVLGLLSYLAERGHRNDL
                     LTHPDGRLFQQSQALNVRTIPLVILNDLDLRPVPRLRRLIRDNNYDIIHFHTKRAHAL
                     SFWLSRGTPRPKCVVTRRMDYPEASNWYTRCLYNRKVEGVVAVSQKISELLVQAGVEP
                     EKIRLIHDGIDPRPFESAANNRDIHTDCIVVGTAAVFEERKGHRFLLEAARRLKAQVC
                     RIQYRLAGDGSLRQSLEKTATRLGLKDDVQFLGFVSDIPTFLSQVDIFVLPSLFEGLG
                     VAVLEAMAAGKAVIASRVGGLAEVVLDGVTGFLVAPGDAEGLANAIAKLAADRSLSRE
                     MGQKGRARLKEHFTLAQMATKNEAYYYGLLKENGDRLPGTEAGSTL"
     gene            complement(2598..3407)
                     /locus_tag="VFN58_08220"
     CDS             complement(2598..3407)
                     /locus_tag="VFN58_08220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET8918013.1"
                     /translation="MSAKNAELRVQPMVSAIVVCFNEERNIGACLDSLRWCDEIVVVD
                     SFSTDRTVEICRQYTERVVQRPWAGYRDQKAFAHSLATKDWVFLVDSDERVSPELRDE
                     IKESLLSYDGAYAGFSVPRLVFYLGRWWWRGGWYPDYDVRLFRRDRATWGGTDPHEKI
                     LVNGKVRRLRNPLYHFSYHDIEDHVQRINRFTSISSRELFHEQRRWRLRDALLRPAFR
                     FFRSYILNRGFLEGFAGFYVALTSAVYVFLKYAKLWELELKRKDDGDRKRS"
     gene            complement(3584..>3992)
                     /locus_tag="VFN58_08225"
     CDS             complement(3584..>3992)
                     /locus_tag="VFN58_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8918014.1"
                     /translation="GHDESLRICMDLDLSFRLLMNYPVAYLDRVVFLYRFHEGNIGKN
                     EELRLTENIRVIQKLLEQFPAAQRELGVRRIAKRISYRYYRLAKKRWHKGQRAEARQA
                     IRESTSLCPFSLKYRFYQFHWGAIARASEPAAL"
BASE COUNT          937 a         1167 c          979 g          909 t
ORIGIN      
        1 gtgacgaatc tgtgactgca tatccgactt taattggcgt ccctcccgtt cgcgcgcccg
       61 ttcgagcctc gtcaaagcgg catcgaggat tttgaagacc ccttgacgct ccgcggcggc
      121 gttgacctcg acctcgcgaa catggaacag gtccggaata ctagaaagaa gcgacaggct
      181 gaagtctcca ctcaactgat actttccttt agcctgccgg acggcggtca aatactgccc
      241 taacagctct tcgtctagtt cgagcctccg actttgtcct tttgcctgcg agcggttgat
      301 aaaaacgtcc agcctgcctc ttgaaatctt ctggcgaata actttccgaa tctcttcctc
      361 gaaagaaaga tattctctcg gcgccctcaa ttgcagatcc aaatgtcgat gattcaagct
      421 tcgcacttgg acggtgactc tggttcctcg aatctggctg gaggcttcgc cgtaacccgt
      481 catgctcttc atagttgccc gtcctggacg attcgtttta atttgtctaa cgacaccgct
      541 acggttccaa cttcatcacc caccagcccg gcggccagat tggcaaggat agccgcttcc
      601 tcgaaactcg cgccgcaggc caacgccaac ccacaaatcg cgaccaccgt atcaccggct
      661 ccggtaacgt cgaacatgtc tctcggttcg gtgggaaaat gtttaaccgc gcagcgcggc
      721 cgaaaaaggc tcatcccatc cggaccttgc gtgatcagca ccgcctcagc ctgccaaatt
      781 tgctccagct tcttacctgc ggcaaggaga ctcttctcat cttgaatctc gattcccgag
      841 gcggcgctcg cttcatcttt gttcggcgtg atcaaagtag gaaggcgata gtgctcgtag
      901 ttttcttttt ttggatcgat cacgcaaatg acctggtttt ttttaaccaa cctcgcgatc
      961 gaacgcagta ggtccgcgtg cacgactcct ttgccgtagt ccgatatgat aacggcatcg
     1021 cacgccgtag cgcgggcgag gacgaagccg cgcagtcgct tcaaggttga ttctccgata
     1081 ggattttggt tttcccggtc caggcgaaca atctgttgat gccgtggccg ggcaataata
     1141 cgactctttt gaatcgtctg aacctgtttg tcagcgaaga tgcccgcagt cgccgctccc
     1201 gcccgacgca ggtcgtgcaa taaccgtttt ccggcgaggt cgcgcccgat gaccccgcaa
     1261 gcggtcactt gagctcccaa tgaacgaaca ttattcacga cgttagccgc accgccaaga
     1321 cgagaactct ccgcagttac ccgcagcacc ggaaccggag cctccggtga aattctctct
     1381 acctcccccc atacgaaacg atcgagcatc aagtccccga cgaccaaaag atgggctccg
     1441 tgaaatccgt tcagaacatc caataggcgc ttcattacag agtcgaacct gcctccgttc
     1501 caggtaagcg gtccccgttt tctttgagga gaccgtagta atacgcttca tttttggttg
     1561 ccatttgcgc caacgtgaag tgctctttca agcgcgccct tcccttttgg cccatttctc
     1621 tactcaggga cctgtcagcg gcgagtttcg caatggcgtt ggcaagtcct tcggcatccc
     1681 ccggcgccac caagaatccg gtgacgccgt caagcaccac ctctgcgagc cctcccactc
     1741 ggctcgcgat cacggctttt ccggctgcca tggcctcgag aaccgctact ccaagacctt
     1801 caaatagcga gggcagaaca aagatatcga cttggcttaa aaatgtcgga atgtcggaga
     1861 cgaagccaag aaactgcacg tcatctttca agcctaaccg ggtcgcggtc ttttccagag
     1921 attgtcttag cgacccgtca ccggcgaggc ggtattgaat ccggcaaacc tgagccttga
     1981 gtcgccgcgc ggcttccaat aagaaacgat gaccttttct ttcctcaaaa acggctgccg
     2041 taccaaccac aatgcagtcg gtatgtatgt cgcgattatt ggccgcagat tcaaacggtc
     2101 gcgggtcaat gccgtcgtga atcaagcgga ttttttcagg ctcgacccct gcttggacca
     2161 acagctcaga aattttttgc gatactgcaa ccaccccctc gacctttcga ttataaagac
     2221 accgggtgta ccagttgctc gcctccggat aatccattcg acgggtcacc acgcattttg
     2281 gccttggggt gccgcgggat agccagaacg aaagcgcgtg cgctcgcttg gtatgaaaat
     2341 gaataatatc gtaattgttg tcgcgtatga gtcgcctgag cctgggtacc ggccttagat
     2401 ccaaatcatt gagtatcacc agagggatcg ttctcacgtt cagcgcttga ctctgttgga
     2461 agagccgccc gtcgggatgc gttaggagat cattgcgatg acctcgctct gcaaggtagg
     2521 acaaaagacc gagaacctga gcctcgccac ccccccaatg cctctcagga tcaatatgta
     2581 agatcttaag caggcggcta tgaccgcttg cggtcaccgt cgtctttcct ttttagctct
     2641 agttcccaga gcttagcata cttgaggaat acataaactg ccgacgtcag tgcaacatag
     2701 aaacccgcaa agccttccag gaagccccga ttcaaaatgt aggagcgaaa gaagcgaaac
     2761 gccggacgga gcaaagcgtc tcttagtcgc caacgtctct gttcatggaa cagctctcgg
     2821 gacgatatcg acgtgaaacg gttgatcctt tgaacgtgat cttcgatatc gtggtaggaa
     2881 aaatgataaa gggggttccg caggcggcga actttgccgt tcaccaaaat cttttcgtgg
     2941 ggatctgtgc cgccccaagt cgcgcgatcg cggcggaata atcgaacgtc ataatccgga
     3001 taccagcccc cgcgccacca ccatcggccc agataaaaca ccaaacgcgg taccgaaaat
     3061 cctgcgtacg caccgtcgta ggacaacaag ctttccttga tctcatccct aagctcggga
     3121 gaaactcgtt catcggaatc cacaaggaaa acccaatctt tcgtcgccag ggagtgagcg
     3181 aaagcctttt gatcacgata tccggcccat ggccgctgca ctacccgttc cgtgtattgc
     3241 cggcaaatct ccacggtgcg atccgtgctg aaggaatcca ccacgacgat ctcatcgcac
     3301 cagcgcaggc tgtcgagaca cgcgccgata ttccgctctt cgttgaaaca gacaacaatg
     3361 gcggagacca tcggctgtac ccgcagctcg gcattcttgg cgctcatgtt tcctaaaaga
     3421 taggggctcg ctttcggaaa atcaaactag agtttgaact ctcccgtgca aggcgctgca
     3481 aggttacgca gattaatccg gagaaagctc gcgtgtacgg gcttgaagat gtttgcacca
     3541 ttcacggtat gctgaccaaa gcgacaccac tacggttcta cagctaaagc gcagctggct
     3601 cactagcgcg cgcgattgcg ccccagtgaa actggtaaaa ccgatacttc aaagaaaacg
     3661 gacatagaga ggtcgactca cgaatcgctt ggcgggcttc ggctcgttgt cccttatgcc
     3721 aacgtttttt ggccagtcga tagtaccggt aagatatgcg ctttgcgatt cgacgaacgc
     3781 cgagctctcg ctgtgcggcg ggaaattgct cgagcaattt ttggatcact cgaatatttt
     3841 cggtcaaacg tagctcctca tttttgccaa tattaccctc gtgaaatcga tacaaaaaaa
     3901 ccacgcggtc gagataagcc accgggtagt tcatcaacag acggaaagat aaatccaagt
     3961 ccatgcaaat acgcaacgac tcatcgtgcc cg
//
LOCUS       DASSWZ010000343         4094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1400703, whole genome shotgun sequence.
ACCESSION   DASSWZ010000343 DASSWZ010000000
VERSION     DASSWZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400703"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFN58_08230"
     CDS             complement(<1..926)
                     /locus_tag="VFN58_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET8918015.1"
                     /translation="MVLVLKNDQMENLLPMGEEIDAIEQAFRELGQGVAMNAPRARLR
                     TPWKEEGGQYYFNNIMGLVPGVKSMALRIDSSFSKEVQVAGSKRRVYPGDYIGLVMLF
                     DMDTCEILALMDDHHISTMRVGATSAVASKYLARKDAKVLGLLGSGEQARTQVTAHLS
                     VRPLTRVKVYSPTKENREKFAREMSRETGVEVVPVASAEEAVRGSDIVSAATNTVDPV
                     VQGRWLEAGMHVTSLVGGDGFLPRKELDDEAVKRAALIVVGYKPQIFIDKQAEFHDRL
                     ERGIVKAEDLHELGELVNGKCRGRKDNQEITL"
     gene            complement(1081..1353)
                     /locus_tag="VFN58_08235"
     CDS             complement(1081..1353)
                     /locus_tag="VFN58_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET8918016.1"
                     /translation="MTYIIAEPCIGVKDTACVDVCPVDCIHPTKNEAEEFEKENKLYI
                     DPEECIDCGACEPVCPVEAIFEEAATPEKWKQFIQIDADWFKKKKG"
     gene            1492..2583
                     /locus_tag="VFN58_08240"
     CDS             1492..2583
                     /locus_tag="VFN58_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET8918017.1"
                     /translation="MIRKLSRIHVPRPLLAIAIIALGVAFPAGAATIDDALKNLRSLS
                     PAQRKAVLEENARKEGEVVWYTSMSLTDFPKIVGAFEKAVPYVKVRANRLTQSTVITK
                     IDTEARAGLFAVDVVGSAPVEMWELKQRGHSAPYLSPELKAFPAGSYDPQGFWSSFEV
                     TPIVLAFNTKLVPQDEAPRSYQDLLQPKWKGKMNMGSDEYAWYSVMLDGMGKANGLEY
                     MKALARQQLHIPGSSSIMRLQLMLAGESAITIAARGRRATEYKEKGAPIDYRLLDPYP
                     AEPNALTLMRRSSHPHASILFIDWILSEEAQSLMAQQIPRLTLRKGVKQIPRHQELYK
                     KDFVFVNPASIGANLNELIASYQQVFNIR"
     gene            2660..3109
                     /gene="ybeY"
                     /locus_tag="VFN58_08245"
     CDS             2660..3109
                     /gene="ybeY"
                     /locus_tag="VFN58_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET8918018.1"
                     /translation="MPVEIIRRDDGKEFSSRKLKKVAQKVLEIVEQDQAELCLVLVGN
                     AEIRKLNAQFRKKDYPTDVLSFPAGDELPTGVQLLGDVIISVAKAKEQAKARRRTLDE
                     ELVTLLIHGIVHLLGYDHERSPKDARIMDRLEKKIYRALCDRGILKV"
     gene            3213..4094
                     /gene="prfB"
                     /locus_tag="VFN58_08250"
                     /pseudo
     CDS             3213..4094
                     /gene="prfB"
                     /locus_tag="VFN58_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          949 a         1106 c         1144 g          895 t
ORIGIN      
        1 aacaaagtga tctcttggtt gtccttgcga ccgcgacact tgccgttcac aagctccccc
       61 agctcgtgca agtcttcggc tttgacgatg ccccgctcga gccgatcgtg aaactctgcc
      121 tgtttgtcga taaaaatttg cggtttgtag ccgacaacga tgagggcggc tcgcttgacc
      181 gcctcgtcgt cgagctcctt gcgcggcagg aagccgtcgc cgccgaccag gctggtgacg
      241 tgcatgcctg cttcgagcca gcggccctgc acgacgggat cgacggtgtt ggttgcggcg
      301 ctaacgatat cactgccgcg caccgcctct tctgccgagg ccaccggcac gacttcgacg
      361 ccggtctccc ggctcatttc gcgggcgaat ttctcgcgat tctctttcgt tggactgtac
      421 actttgaccc tggtgagcgg ccgtaccgat aaatgagccg tgacttgcgt tcgggcttgt
      481 tcaccagaac cgaggagtcc caggactttg gcatctttac gtgcaaggta tttgctggca
      541 acggcgcttg tggcacccac ccgcatggtg gaaatgtgat gatcatccat gagcgctaaa
      601 atctcacagg tgtccatgtc gaacagcatg acgagaccga tatagtcgcc aggataaacg
      661 cggcgcttgc tgccggcgac ttgcacttcc ttggaaaagc tggaatcgat gcgcagtgcc
      721 atggacttca cgccgggaac aagccccatg atgttgttga agtagtattg tccgccttct
      781 tccttccaag gggttctcag ccgggcgcgc ggcgcgttca tcgcaacacc ctggcccagt
      841 tcgcgaaagg cctgctcgat ggcgtcgatc tcttctccca tggggagcag gttttccatc
      901 tgatcgttct tgagaactaa aaccatagag cttgctcctt ttgcgttgtg ctgagaactc
      961 gggtcttatg gcctgcgtac aacagagatt aacgaagagt caagaaggcg aaaaaaagaa
     1021 aggctgaggg aatttcctca gcctttcgac cggatttgta caatcacgcg gacgggagag
     1081 tcagcctttc tttttcttga accagtcggc gtcgatctga ataaactgtt tccacttctc
     1141 gggagtcgcc gcctcctcaa agatcgcttc gacagggcag accggctcgc aagccccaca
     1201 gtcgatgcac tcctcaggat cgatatagag cttgttttct ttctcgaatt cttccgcctc
     1261 gttcttggtc ggatggatgc aatcgaccgg acagacatcg acacaagcgg tatccttcac
     1321 gcctatgcag ggctcagcaa tgatataggt catctctcat tcctcctaag tcgaactttc
     1381 agtttagtag caaatcgact agcccaagtc aataaaatct acgcctttcc tgaggcttac
     1441 cttgaccggt ctttgcggaa gtgttacacg gggccaactc cgagaaacga gatgatcaga
     1501 aagttatcgc gaattcatgt gccgcgtccg ctgttggcga tcgccattat cgctttgggt
     1561 gttgcttttc cggccggcgc cgcaacgatt gacgatgctc tgaagaacct ccgtagcttg
     1621 tctcctgcgc aacggaaagc tgtgttagag gaaaacgcgc gcaaggaagg cgaggtcgtc
     1681 tggtatacct cgatgagtct gaccgacttc ccaaaaattg tcggtgcttt cgagaaagct
     1741 gttccctacg tcaaagttag agccaatcgg ctaacacaat ccactgtcat taccaagatt
     1801 gacaccgaag cgagagccgg gctcttcgcg gtagacgtag taggatccgc gccggtggag
     1861 atgtgggagt taaagcagag aggtcattcg gccccttact tgtcgcctga actcaaagca
     1921 tttcccgcgg gttcctatga cccgcagggt ttctggtcct ccttcgaggt aactcccatc
     1981 gtgctggctt tcaataccaa gctggtgcca caggatgagg ccccccgaag ctatcaggat
     2041 ttgctccagc ccaagtggaa aggcaagatg aacatgggca gcgacgagta cgcctggtat
     2101 tcggtgatgc tcgacggaat ggggaaggcg aacgggctgg agtacatgaa agcgctggcc
     2161 cggcagcagt tgcatatacc gggttccagc agcatcatgc gcctgcagct catgctagcc
     2221 ggcgagtcgg ccatcaccat tgccgcgcgc ggtcgacgtg ccactgagta caaagaaaaa
     2281 ggagcgccca ttgactatcg tctcttggac ccctaccctg cggagccgaa cgcgttgact
     2341 ctgatgcggc gatcgtccca tcctcacgcg tcgatcctgt tcattgattg gattttgtcg
     2401 gaagaggcgc agtcgttgat ggcacagcag attccgcggc taacactgcg gaaaggcgta
     2461 aagcagatcc cacgccatca agagctttat aagaaggatt tcgttttcgt aaacccggcc
     2521 tccatcggag ccaatctcaa tgagttgatt gcttcttacc agcaggtctt taacatccgt
     2581 taggaattgt agagcgggtt tcaagccctc ccctattctc tcgttcgcaa gatcagcaag
     2641 gaaaagttac actcccgcga tgcctgttga aatcatccgc agggatgacg gcaaagagtt
     2701 ttccagtcgc aaactgaaga aagtcgccca gaaagtacta gagatagtcg agcaagatca
     2761 agccgagttg tgtttggtgt tggtgggtaa cgcggagatt cgaaaactaa atgctcagtt
     2821 tcgcaaaaag gactatccga cggacgtgtt gtcgtttcct gccggggacg agttgccaac
     2881 cggcgtgcag ctgttagggg acgtcattat ctcggtggca aaggctaaag aacaggccaa
     2941 agcaagacgg cgaacattgg acgaggaact ggtgacgctg ttgattcatg gcatcgtgca
     3001 tctgcttggg tatgaccatg agcgctcgcc gaaagacgcc cggatcatgg accgtctgga
     3061 gaagaagatc tatcgcgcgc tttgtgaccg ggggatatta aaggtataat tgcttatcgg
     3121 cgcttcaggc cgatcgctta acagcgacga ctgagcttgg gtcagccaag acacagcgaa
     3181 tacaatgaat tgaacggaag agaaacggat aaatgctgga agaaaccaaa gaacaacttg
     3241 cccacctgga agagcgactc gaacttttgc ggaggcgtct ttgacctcga cggaaaacag
     3301 aagcgggtcg aggagctaaa caaggtcact tcccagcctg atttttggaa cgacagcgaa
     3361 aaggcccaag gtattctcaa agagcaagcc tccctcaaag atgtcataga gtcctgggaa
     3421 aaatatcgat cggctcttga agaagcgcgg ttctttctcg acgtagccaa agacgagaag
     3481 agcgaagagg cgctcagtga agccgccgcg aaggtcgccg aggtggcgca agggatggcc
     3541 cagaccgaac ttacgcaact ccttggcggg cctgacgacc gacgcaatgc gattgtaact
     3601 cttcaccccg gtgccggtgg aaccgaagcg caggactggg cggagatact gctgcgtttg
     3661 tatctccgct gggcggaccg ccgcggctat cggaaagaga tcctggaata tcagccgggc
     3721 gaagaagccg gcgtcaagag cgttaccttc acggtggaag gcgagtacgc ctacggctat
     3781 ctaaaggctg aagccggtat ccaccggctg gtgcggatct cgccgtttga cgcgaattcc
     3841 cgacggcaca cttccttcgc ttcggttttc gtctatccgg aagtggacga caccatcaaa
     3901 atcgaggtca acgaagcgga tctgcgggtc gatacctatc gttccagcgg cgccggcggc
     3961 cagcacgtga acaagaccga ctcggcggta cgcctgactc accttcccac cgggatcgtg
     4021 gtggcctgcc agaacgagcg ctcacaacat aaaaaccggg caatggcgat gaagattctg
     4081 cgctcccgtt tgta
//
LOCUS       DASSWZ010000344         5739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1089580, whole genome shotgun sequence.
ACCESSION   DASSWZ010000344 DASSWZ010000000
VERSION     DASSWZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089580"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFN58_08255"
     CDS             complement(<1..589)
                     /locus_tag="VFN58_08255"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HET8918019.1"
                     /translation="MRSFVLKIYHYPLLPRIRTGLRISKAEQEFNSLRYLNQQGVPAA
                     EAVAFGVERTRLGFVRSCFVITGFVEGAVNLSRWRSESSQQQPPHTDKNHFLLKQLGT
                     MFRRLHDVRFFLFTAKTKNILIRNESTRSPEIFFLDVPYARTLRWRPVARWARGRDLG
                     LFLGNFYPALAESETTAFYKGYLPDPLGGSAAALRR"
     gene            1201..2325
                     /locus_tag="VFN58_08260"
     CDS             1201..2325
                     /locus_tag="VFN58_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET8918020.1"
                     /translation="MGAVSIVLPGRLSGQPYKELGELTQEKSNPAPEENSRGPALWGW
                     YTYLSLAIGAVILVGLAGLLDPKEIWREVIAANKKLLLLGALAHYATYPVRGLRWRRG
                     LIHFPVQGGPGKFGLIVFFYTFVDNLVPAKLADLYGAHLARINCGVRRSAALGSIVFL
                     RTADAWTVLLLAFVASWVLFSATMAASVVWTLVAGGIIAVATTSVMLVFIFLKKSSLT
                     WLPAKAREMVHAFHTGMWPNTSEIAPIAMLTLLVWALETLWVFLLVLAFGVIPSFEEA
                     VFLTMVPILASAFPLTPSGAGLVELTLFTCLRLVGVASPVAASVTVVNRLIDYWLHIV
                     LGVLIWAIRHVIGLRTWNEVPLESVEPPEAVLREEAVRGG"
     gene            2315..3820
                     /locus_tag="VFN58_08265"
     CDS             2315..3820
                     /locus_tag="VFN58_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET8918021.1"
                     /translation="MEVNSVFLIRVSSQADKVDHPQFFDPSYPLKSIQAGLERYGDVA
                     VSLLDCWIHPMDVPQMVEHVQRFQPDLVVVSASSFDITVAKRLMVSLKRHDGAPLVVG
                     IGQGYYLQHDFFMDDSEYEYDTVLLGEPEEEFFKLFEWIRESKPTDESWKDHYRRLYV
                     DGKRYTVNDPDNLAFANYTYDELRAYKSIYPVKLSKAVVWGYLIATRGCPYDCTFCSE
                     VMRVSIGKKLRGRLPANIVDEMEHLARQGVNICSFQDDSFSSNRNLVKALCEELIARD
                     SKMPWMCRARIDEVNYETLALMKKAGCVMIGFGVESGSQRIVDDMIKQYRDRPWQDLC
                     RQTFKWTRELGIGTNAYYIIGNPTETREEIEKTIELALELNTDTIQVHFYTPYPGSTD
                     WEKYKDQIEKSDAEHMFHYAAPKFNPSAVFSEELVALRSKFYRRYILRPSFAIPHFWG
                     HAGFYWNNPDVFWSLVGIRKVFGTSAKGSTQEDTNGVGPHRTATMRESSPG"
     gene            3768..5027
                     /locus_tag="VFN58_08270"
     CDS             3768..5027
                     /locus_tag="VFN58_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET8918022.1"
                     /translation="MALVRTVPRRCVNLPPGSLRTLISCVLINKVEEGPCIEEFHREF
                     GKWLGVPYVFGTSSGRSAFQLALESLGLEKGSEIIFPVLTFPVMPMVAKLLGYNPVFC
                     EVDPVTFNSGPEHIAPKISNRTGAVLATHLFGQPCPIRDVMELARARKIRVLEDCAHA
                     CGVKVGGQQVGTFGDVGVYSFAEGKNMPCFGGGAIATADEKIAKRAKQILSESRKPRK
                     MAVITEALTIWAKWLMTRPLIFGLTVYPVLRLKLRMGQPLMDSAVGNELLDEFVSTKP
                     GITRLSNLQAAIGLRQLQRIDHFNEGARRNAHRLTEKLGEVPGIKAPSSTDGDHIYVY
                     YPLTVNPERRDDLRHYLLRHGIDSKTTDMADCSTLKPFLSTGNGGQRNGPSEASILEI
                     CVYPVIPEAKMFEIARTIRHWGGLQEQ"
     gene            5336..>5739
                     /locus_tag="VFN58_08275"
     CDS             5336..>5739
                     /locus_tag="VFN58_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET8918023.1"
                     /translation="MLLLAVLGVLLYVGCIGLFDLWYPDEPDIAEVAQAMFVSGDWIA
                     PRRMGVIFVDYPPLIYWAGSISSTVLGGMNQFTLRLPSALAAVGLVLLTCAVGSRWYD
                     GKTGLWAGFLLLTFSQFVLEAVGYRTDMLFSF"
BASE COUNT         1269 a         1484 c         1572 g         1414 t
ORIGIN      
        1 aacggcggag ggctgcggca gagccgccca aagggtccgg cagatagccc ttatagaacg
       61 cagttgtctc gctctcagct aaagcaggat aaaaattgcc gagaaacaat cccaagtccc
      121 gcccccgcgc ccatcgcgcg acaggccgcc agcgcaatgt tctggcgtag ggcacgtcaa
      181 gaaaaaaaat ctccggggat cttgtcgatt cgtttcggat cagaatgttt ttcgtcttag
      241 cggtaaacaa gaaaaatcgc acatcatgaa gccggcgaaa cattgttccg agttgtttta
      301 agagaaaatg attcttgtcg gtgtggggcg gttgctgctg gctgctttca gatctccatc
      361 gggaaagatt aacggctcct tcgacaaagc cggtgataac aaaacaggat cgtacgaaac
      421 ccagccgagt ccgctccacg ccaaaagcca ccgcctcggc agccggcaca ccctgttgat
      481 tgaggtaacg caagctgttg aactcttgct cagccttaga aatgcgaagc ccggtcctga
      541 ttcttggcag aagagggtaa tggtaaattt tgaggacgaa cgaacgcatt gattcttcct
      601 ggcgcccttt tgcctgcctc cgcagtaaaa caatccgggt tctacgctcc gacttgaccg
      661 gttcaccgtc ggcattcaag aacgcatcat agctcggata ttcactcgcc ggaaaatagc
      721 actcatagga cgggcacacg aaaaagggcc gcctgcaatc tgtagtcgag tgcagcgatg
      781 ccgttgccat agattgaatc gtcataattg ccgggccgcc ttttcatttg tttctcgcca
      841 caaccacggt taaactaaca ttggaactga caatcgctat agggaacctc ttattttcgg
      901 ttccgacttc ctggttaaga cgtccaaaat ttctaccata attagcagga atctgaaaac
      961 cgactaaact attataagat cagtcaccaa ggattgaaat cacacgcaac gcctctggca
     1021 aaccttttca atttttaggc tgtcttccct tcctcaagtt gtcgtagacc gtttcacaac
     1081 atcacgtaac tccggcgctt agccggcctt gaagggacgg aatctcgagc aggttgaatt
     1141 tctgaaacgc atcagctatg tgaagttaaa aaggaatgac ctgctcatgg cgccaaggaa
     1201 gtgggcgccg tatccatcgt tctccccggt aggctcagcg gccagcccta caaggagctt
     1261 ggcgagttga ctcaagagaa aagcaatccg gcaccagaag aaaactcacg agggcccgcg
     1321 ctgtgggggt ggtataccta cctgagtctt gccatcggag cggtcattct ggttggtttg
     1381 gccggcctgc tggatccgaa ggaaatctgg cgcgaggtca tcgcggcgaa taaaaagctt
     1441 cttttgttgg gggctctggc ccattatgca acttatccgg tgcgcgggct gaggtggcgc
     1501 aggggtctga ttcattttcc cgtgcaggga gggccgggca aattcggtct catcgttttc
     1561 ttttacactt tcgtggacaa tctcgtgcct gcgaagttgg ccgacctcta cggagcacac
     1621 ctcgcacgta tcaactgtgg ggttcgacgc tccgcggctc tgggttctat cgtctttttg
     1681 cgcacggcgg atgcgtggac ggtccttcta cttgccttcg tagcctcctg ggtcctgttt
     1741 tcggctacca tggcagcatc cgtggtttgg accctcgtcg ccggtgggat tatcgctgtc
     1801 gcgacgactt cagtaatgct ggtttttatt ttcttgaaga aatcgtcact cacatggttg
     1861 ccggcaaagg ctcgggagat ggtgcatgcc tttcacaccg gtatgtggcc caatacgagc
     1921 gagattgctc ccattgccat gctcacactg ctcgtctggg cgctggagac gttgtgggta
     1981 tttcttctcg tcctggcctt cggcgtgatc cccagctttg aggaagcggt attcttgact
     2041 atggtaccga tactggcctc agcgtttccg ttgactccct caggggcggg tcttgtggaa
     2101 ctcaccctat tcacttgcct tcgtctggta ggtgttgcgt caccagtggc ggcctccgtc
     2161 accgtcgtaa atcgcttgat tgattactgg cttcacatag ttctaggcgt gctgatctgg
     2221 gcgatacgtc acgtgattgg cctgcgtact tggaacgaag tgccgcttga aagcgtagaa
     2281 ccgcccgagg cggtgctcag agaggaggcc gttcgtggag gttaattctg ttttcctaat
     2341 tcgcgtcagt tcccaagcgg acaaggtgga ccatccgcag ttctttgacc catcctatcc
     2401 cctgaaatcc attcaagcgg gtttggagcg gtatggagat gtcgccgtaa gtctattgga
     2461 ttgctggatc catcccatgg atgtcccgca gatggtagaa catgttcaga ggtttcaacc
     2521 cgacttggtc gtggtttcgg cctcgagttt tgatattaca gtggcgaaga ggctcatggt
     2581 ctcgttgaag agacacgacg gtgcgccact cgtggtgggt atcggtcagg gatactatct
     2641 tcagcatgat ttttttatgg acgattcgga atatgagtac gacaccgttt tgctcggaga
     2701 gccggaagag gagttcttta aactctttga gtggatacgc gaaagcaaac caacagatga
     2761 aagctggaaa gaccactatc ggcggctcta tgtagatggg aaacgttata cagtgaacga
     2821 tccagacaac ctcgcctttg ccaactatac ctacgacgag ctgcgggcct ataaatccat
     2881 ctatcccgtg aaactctcca aggcagtggt atggggttat ctcattgcca cgcggggatg
     2941 tccctatgac tgcacctttt gcagcgaggt gatgcgcgtg agcatcggaa aaaaactccg
     3001 cggccgtttg cctgcgaaca ttgtcgatga gatggagcat ttggcgcggc agggggtcaa
     3061 tatttgttct tttcaggacg acagcttctc atccaatcgc aacctcgtta aggccctctg
     3121 tgaggagctc atcgcccggg attccaagat gccttggatg tgccgggcgc ggatcgatga
     3181 ggtgaactat gagacccttg ctctcatgaa aaaggcaggt tgcgtcatga tagggtttgg
     3241 ggtggagtcc ggcagccaga ggatcgttga cgacatgatc aaacagtaca gggatcgacc
     3301 ctggcaggat ctctgccgtc agaccttcaa atggacgcga gagctgggta tcgggacgaa
     3361 tgcctactac attattggaa atccaactga gacgcgggaa gagatcgaaa agaccatcga
     3421 actcgccttg gagctcaaca ccgacacgat tcaagtacat ttctatacgc cttaccctgg
     3481 ctctacagac tgggagaagt acaaagatca gatagagaag agcgatgcgg agcatatgtt
     3541 ccactatgcc gctcccaagt tcaatccatc agccgtattc agcgaggaac tagtggcgct
     3601 ccgctcaaaa ttttatcgcc ggtacatctt acggcccagc tttgccattc ctcatttctg
     3661 gggacacgct ggcttctact ggaataaccc tgatgttttt tggagtctcg tggggatccg
     3721 gaaggtgttt ggcacttcag ctaagggctc tacccaggag gacaccaatg gcgttggtcc
     3781 gcaccgtacc gcgacgatgc gtgaatcttc ccccgggtag tttacgcacg ctcatcagct
     3841 gcgttctcat caacaaggta gaagagggtc cctgcatcga agaattccac agagaattcg
     3901 ggaagtggtt gggtgtccct tatgtatttg gcacttcatc gggccgatct gcattccagt
     3961 tggctctgga gtcattgggg ctcgaaaagg gttcggagat catctttccg gttctgacgt
     4021 tccctgtgat gcccatggtg gccaagttgc tgggctacaa ccccgtcttt tgtgaggtgg
     4081 atccggttac tttcaactct ggtcccgagc atattgcgcc taaaatatcg aatcgaaccg
     4141 gcgccgtatt ggctacccat ctttttggcc aaccctgtcc gatacgcgat gtgatggagc
     4201 tcgcgcgggc gcgaaagatc cgcgtcttgg aagattgcgc ccatgcctgt ggagtcaagg
     4261 tagggggcca gcaggtgggt accttcggag acgtgggtgt gtatagtttt gccgagggga
     4321 aaaacatgcc ttgctttggg ggtggggcga ttgcgactgc tgacgagaag atcgcaaagc
     4381 gagcgaagca gatcctgtcc gaatcgcgga aacctaggaa gatggccgtc atcacggagg
     4441 cactcaccat ctgggccaag tggttgatga cccggccact gatctttggg ctgacggtct
     4501 atccggtttt gagacttaag ctccgtatgg gtcagccgct gatggattcc gccgtaggaa
     4561 acgagctgct cgatgagttc gtcagcacaa aacccggaat cactcggtta tcgaaccttc
     4621 aagcggccat cgggctccgc cagctccagc gaatcgatca ctttaatgaa ggagcccggc
     4681 gcaatgctca ccgcttgacc gaaaaactcg gcgaggtgcc cgggatcaag gctccgagtt
     4741 ccactgacgg tgatcacatc tacgtctatt accctcttac ggtgaatcct gaacggcggg
     4801 acgacctgcg ccattatctc ctgcggcatg gaatcgattc aaagactacg gatatggcgg
     4861 attgctccac tctcaaacct ttcctgagca cagggaatgg aggccagcgg aatggcccga
     4921 gtgaggcatc gattttagag atctgtgtat atccggtgat accggaagcc aaaatgtttg
     4981 agatcgcccg cacaattcgc cactggggtg ggttgcaaga acaatagaat ctctattcca
     5041 taaatccaca tagctgtgcc aacggagatg aataacgtat aggcgaacct actaaccagt
     5101 ttaccaccga gtccagcaca ccgatattca gctttcgact cgccatcttt actcatttgg
     5161 gagatatctt agtaatcaat gacaaggtcc cggagccgaa gacgacgcgc ctaagtttcg
     5221 gttcttcgcc ggattaaaac agagttgctc aacaatgact agtgatcgtt ctttacacga
     5281 gagtttgtat gtctcaagcg aatccccgcc gccgagtttt ctcgtagagc accggatgct
     5341 tcttctcgct gtcctcggcg ttctcctcta cgtcggatgc attggccttt ttgatctgtg
     5401 gtatcccgat gaacctgaca tcgccgaggt tgcccaggca atgtttgtaa gcggtgactg
     5461 gattgcgccg aggcgcatgg gagtcatatt tgtcgattat ccgccgctca tttactgggc
     5521 gggcagtatc tcttccactg tcctgggtgg gatgaaccag ttcactctgc ggctgccttc
     5581 tgcgctggct gccgtcggcc tcgtgctttt gacctgtgcc gtggggtcgc gttggtacga
     5641 cgggaagacc ggactttggg ccggttttct tctgttgact ttctcccagt tcgtgctgga
     5701 ggcggtgggt taccgcaccg acatgctgtt cagcttctt
//
LOCUS       DASSWZ010000345         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_353590, whole genome shotgun sequence.
ACCESSION   DASSWZ010000345 DASSWZ010000000
VERSION     DASSWZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353590"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFN58_08280"
     CDS             <1..252
                     /locus_tag="VFN58_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET8918024.1"
                     /translation="ETAELADNSSLPDEEAIAGEAKRKADLTLEAMPNKQRTAFLLRN
                     HQGLSIPDIAKVMKTTEGTVRVYLHRAVAALRQSLMEFV"
     gene            264..800
                     /locus_tag="VFN58_08285"
     CDS             264..800
                     /locus_tag="VFN58_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET8918025.1"
                     /translation="MKEHSDTLRTACKDFEEDLVLYYYGDGSEKERIRVQGHLQTCSS
                     CREFLEDLRKLLPQMAKPSELPPTFWEDYYDEMVQKLAVHEERQAWWKNWFAPMRTWM
                     MPAFGTAVVAALAIGLVISRGHWHYPFSQTQEQIPQEILTDTNQLEFFKSMDMLETLS
                     SLEAQDGTKVQPGNNQHG"
     gene            793..1128
                     /locus_tag="VFN58_08290"
     CDS             793..1128
                     /locus_tag="VFN58_08290"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HET8918026.1"
                     /translation="MGNTMDGGDHAKSNGLVKMLKRTIGFLGVCLVLLLPAYGWARDG
                     GDNWRRLSPKEKENVQRNYQRWQNLPPQDKEHLREEWDRFKNLPPDQRDNLRRRYDEQ
                     RKQGPGNKR"
     gene            complement(1352..2479)
                     /locus_tag="VFN58_08295"
     CDS             complement(1352..2479)
                     /locus_tag="VFN58_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET8918027.1"
                     /translation="MAKQGFRILDSDMHVMEPPDLWERYIDSKYRSQAPRGVTSSNVR
                     DLRMVYPDGRDWARKTIRQPRPDPGHNFERNQEVYRSYAARGWTAEVQLEAMDIEGID
                     VAVLYPTRGLRALVVDDMEAGFAAALARAYNDWLYDFCEQDPQRLVGAGMISPYDMSN
                     AVDEARRCAQELGFRAVFLRANPLVNHQWHDDYYEPLWDALEELGLSLGFHESTGTGK
                     HQIGERLEPNLMLRRIYAQPMEQMMALGSFCAGGVLARHPRLRIAFLEANCSWLPWLL
                     WRLDEGWEREGDVWAPDLTLAPSEYFKRHCVVSVEPDEITVKQVIDTIGSNNIVFSTD
                     YPHGDSKYPEAVDRFLQLPLANEAKRNILWDNCAAYYALQD"
     gene            2629..>3111
                     /locus_tag="VFN58_08300"
     CDS             2629..>3111
                     /locus_tag="VFN58_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET8918028.1"
                     /translation="MTTTKRLPAFEKFIQDVRAAWAELPDTEKRMKRVKVLLEGLVQD
                     STIREHSKHWPSTEGHKNLLLYEDPDYQFVVNAVVRTPGRDGRIHDHAHAWTAYGVLD
                     GTERLERYRRVDQGEKAGYALLELESLTEGYPGKVDLVPPFAIHSEKGGPTRSVGVIV
                     R"
BASE COUNT          750 a          791 c          878 g          692 t
ORIGIN      
        1 gagactgccg agttggccga taactcttcg ttgccggacg aagaggcgat tgccggagaa
       61 gctaaacgta aagctgatct cacgctggag gcgatgccca acaaacaacg gacggcattc
      121 ttgttgcgca atcatcaagg gttatcgatt ccggacattg ccaaggtgat gaaaactacg
      181 gaaggaacag ttcgcgtcta tcttcatcgg gcagtggcgg cgctgagaca gagtctgatg
      241 gaattcgttt agggagaatg gtcatgaagg agcatagcga cacgttgcga actgcttgca
      301 aggatttcga agaagatttg gtgctgtatt attacggcga tggttctgag aaagagcgca
      361 tccgggtgca aggccacttg cagacgtgtt cgtcttgtcg ggagtttctc gaagacttgc
      421 gaaagttact gccccaaatg gctaagccga gcgagcttcc accaacgttt tgggaagatt
      481 actacgatga gatggtccag aagttggcgg ttcacgagga acgccaggct tggtggaaga
      541 actggtttgc gccgatgcgg acatggatga tgccggcttt tggtaccgcc gtggtcgcgg
      601 ctctggccat cggtctggtg attagtagag gccattggca ttatcctttc agccaaactc
      661 aagaacagat ccctcaggag atcctgacgg atacgaacca gttggagttt ttcaagtcga
      721 tggacatgtt ggaaacgctg agctctctcg aggcccagga tgggacgaag gtacagccag
      781 gaaataacca gcatgggtaa cacgatggat ggcggtgatc acgcaaagag caatgggctg
      841 gtcaaaatgt tgaaacgaac tataggattt ttgggtgttt gcttagtcct gctgctgccc
      901 gcctacggct gggcaaggga tggcggcgat aattggcgcc gtctcagtcc caaagagaaa
      961 gagaatgttc aacgcaacta tcaacgctgg caaaatcttc cgccgcaaga caaagagcac
     1021 ctgcgcgaag agtgggaccg gttcaaaaat ctgccgccgg accaacgcga caatttgcgg
     1081 cggcgctacg atgagcagcg taagcaaggt cccggtaata aacgatagaa attctgtccg
     1141 ttttttctac tccgggtagg actcactacc gaagtcagtc attcccgcat gcttttagcg
     1201 gtggctcgac tgggctcgcc acactgagct tgtcgaagtg gaatccaggg gatgttcgaa
     1261 ctggaccccc gataaaaaca ctcgggggtg acgaccttgg aataaattcc gatcccctcg
     1321 ggggcttgct gcgagtatag atcattgctt attagtcttg cagggcgtaa taagcagcgc
     1381 agttgtccca caggatattt cttttggctt cattcgcgag gggtaattgg aggaagcgat
     1441 caaccgcctc aggatacttg gaatcgccat ggggatagtc ggtggagaaa acgatgttgt
     1501 tgctgcctat ggtgtcgatc acctgcttga cagtgatttc atcgggctct accgaaacaa
     1561 cacagtgacg cttgaagtat tcgctcggcg ccaaggtgag atccggtgcc cagacgtccc
     1621 cttcccgttc ccacccttca tccagccgcc acaatagcca cggcagccaa ctacaattgg
     1681 cttcgagaaa agcgatcctc agtcttggat gacgggcaag cacgccgccg gcacaaaaac
     1741 ttccgagagc catcatttgt tccatcggct gggcgtagat gcggcggagc ataagattgg
     1801 gctccaatcg ctcgccaatc tggtgtttgc cagtgccggt ggactcatga aatcccaatg
     1861 acaagccaag ctcttccaaa gcatcccaga gcggctcgta atagtcgtca tgccattgat
     1921 gattaacgag cgggttagct ctcagaaaaa ccgcgcggaa acccaattcc tgcgcgcacc
     1981 gacgtgcttc gtcgactgcg ttgctcatgt cgtagggtga gatcattccg gcgcctacca
     2041 ggcgctgcgg atcttgctcg caaaaatcgt agagccaatc gttgtaagcc cgtgccagag
     2101 cagcggcgaa gcccgcctcc atgtcgtcta ctactagggc tctcaggccc cgagttggat
     2161 agagcacggc aacgtcgatt ccttcgatat ccatggcctc aagctggact tccgccgtcc
     2221 atccgcgcgc tgcgtaactt cgatagacct cctggttgcg ttcaaagtta tgacctggat
     2281 ctggccgggg ctgtcgaatt gtctttcggg cccagtctct gccatcgggg tagaccatgc
     2341 ggaggtcacg cacgttagag ctggtcacgc cgcgcggcgc ctgagatcga tatttgctgt
     2401 cgatgtaacg ctcccaaaga tccggcggct ccataacgtg catgtcacta tccagaatcc
     2461 taaaaccttg cttggccatc tggcatcctc ctggtcgagc gatagtatag cgaatcggtg
     2521 gtgtcaacaa accttgaatc ataacctggc taatcgaaga agctggcatt ctattttttg
     2581 gcgcgatata gctgagatgt ttttctgaca aagacaggag acgaatttat gaccaccacc
     2641 aaacgccttc cagcatttga aaagtttatc caagatgtac gagcggcatg ggcggaacta
     2701 ccagatacag agaaacgcat gaaacgggta aaagtcttac tcgagggact ggtgcaggat
     2761 tcgacgatac gggagcattc taaacattgg ccatccaccg agggtcataa aaacctgttg
     2821 ttgtatgaag atccagatta tcagttcgtc gtcaacgcgg tcgtgcgcac acccgggcgc
     2881 gacggccgga ttcacgatca tgcgcacgca tggacagctt acggcgtgct cgatggtacc
     2941 gagcggctcg aacgttaccg tcgcgtcgat caaggggaaa aagccggata cgcgctgctc
     3001 gagctagaaa gcctcactga gggctatccc ggcaaggttg atctcgtgcc gccttttgcg
     3061 atccattccg aaaaaggtgg tccgacaagg tcggtcggag tgattgttcg c
//
LOCUS       DASSWZ010000346         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_891139, whole genome shotgun sequence.
ACCESSION   DASSWZ010000346 DASSWZ010000000
VERSION     DASSWZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891139"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFN58_08305"
     CDS             <1..224
                     /locus_tag="VFN58_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET8918029.1"
                     /translation="LERERIIQPIMRRKLKFYSPDQVDRVRVAHLLIEEMGVNLEGAE
                     VALHMREQMIAMQRQFNELLRLLGQQFKK"
     gene            complement(407..1024)
                     /locus_tag="VFN58_08310"
     CDS             complement(407..1024)
                     /locus_tag="VFN58_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET8918030.1"
                     /translation="MKGFPLLHVLINLFCVGFSTKKDAGMALAFVSHKNATEIFFKKG
                     EQMKRKKPVVAAIILSGAVGFASSPLWSQELPGERRQSAPNQTRPGRDQDVPGVNQLG
                     TPELSKNDMRAVEEALQTKGYKPGKIDGVADDEAREAIRAFQKDNGFSVTGMVDQKTA
                     DRLGVRISSKSGGSSTGGTSGRSGAGSDSKMTKPGDDPPTGTRKY"
     gene            complement(1253..2263)
                     /locus_tag="VFN58_08315"
     CDS             complement(1253..2263)
                     /locus_tag="VFN58_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET8918031.1"
                     /translation="MVKTFDRLLVSFALFYIVALPCEAPAQLAKVTTSYVSDSAGVLP
                     LWIAKESGLFSRNGLDVQAVRVHASVGVMALMSGELQFVQASGPVVVESALRGSNAVY
                     VACGMSTLDFVLLSRPEIKTAEQLKGATVGLSAFRGANPTATRFALQKLGLAPKDVSL
                     IVVGGVTERIASLRLGKIQATLFSPPQNVVAQREGFNVLADVAELGLPFVHIGLATTR
                     SFIRDNPETVRRYVKSYVEAIHLMKTDRETGIKVLAKYLGQIKDRDILVKTYDGAVPD
                     NKFPRKQYPSAAGLQMALDLLAEENPKAKTAKPQDFVDARFIKELDDSGYIDGLYRSK
                     GN"
     gene            2497..4131
                     /locus_tag="VFN58_08320"
     CDS             2497..4131
                     /locus_tag="VFN58_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET8918032.1"
                     /translation="MAETNIAETVVEYLSSKNVRHIFGVLAHTSFALGDAIAKRPHIR
                     FVNSQHEGGAGNMALGYARAAKKPAVCLVSAGGGATNIVTAVAQAYKEAVPLFVISSD
                     INTASSSKGAWSSWHGMEHVRLFEPITKQSLKLERVEEIGSVLDSLFRLTTRGRQGPV
                     FLIIPADLQAVALPEPLMFSPTAAPAAPSIDSRSTEMAVDLLLKATAPVVLVGTGVDW
                     SGAQREVQELAELLSLPVATSYTAKGVFPENHPLALGCLGVGGRPYAKELFLKSDLIL
                     ALGTTFSEGTTMGFGHRTIPDQAKIIQIDIDPDELGRNYPTQLAMQADVKAALGAIID
                     GIKSKRPSAPAAGGGGRAREIERAKQAWRSDLDKKTANGNLTYDSVLKTLNELLTGTE
                     ILVTSGMTGDLLRNIDAATPIIHAGEFRAIGTAFATSLGVKLGLPDARVVCVTGDGSF
                     MMEQQELATARLHNIPVLAIVLRNNAYGGMKRDQLHHYGGRVIGTELFVPDLARMAEL
                     FGAKGYTITRPEEVRPVLQKSLESDDFVVVDVKVDK"
     gene            4254..5363
                     /locus_tag="VFN58_08325"
     CDS             4254..5363
                     /locus_tag="VFN58_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET8918033.1"
                     /translation="MVIFSMKKLMVTVLAALHLLFVAALTIESVQAAEAKQAAAKRHK
                     LPATLETTQWGWLDPNESPKLTVDSGDIVSIETMMHSHNKIQPGTTMDEIVALRKANP
                     GGGPHSVTGPIYVNGAEPGDVMEIRILRIVPKPFGVNFNLPGKEFPTIGALATEFPEG
                     FVKYFNFDSKNRQAEFKPGVMIDLQPFPGTLAVGIDPDDPAPRKGGVKDSMAPVSTLR
                     PWKNGSNMDLNELQEGSIIYIPIFLKGGLVWTGDSHCRQGNGEVNLTALECSFREIAL
                     QLIVRKDMKLEWPRAETKTHWILMGFDEDLNKAMVSAVRETVDFLANQKMVPLDRYEA
                     YSITSMTADCRVTQVVDVRKGVHCMVPKSIFTKKK"
     gene            5367..>6012
                     /locus_tag="VFN58_08330"
     CDS             5367..>6012
                     /locus_tag="VFN58_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET8918034.1"
                     /translation="MLCALAFFLLWTAAAWGAEPLPVVTTSTDLKALVEVVGGERVRV
                     ESLASPLQDPHAVEVKPGQLTKLKVAALLVRIGLDHEPWLGRALRTVGDSRFTRESPH
                     YLDTSKGIQLLQAETPRVRADKGAHLHGFGNTHYWLDPENGRPITAAILTALLRLAPA
                     DRPYFEANRKRFLEQLDDRLKGWSQTMAPYKGTRLVAVHETWPYFAQRFGLTIVA"
BASE COUNT         1335 a         1637 c         1680 g         1360 t
ORIGIN      
        1 ccctggagcg ggaacgcatt atccagccta ttatgcggcg caagctaaaa ttttattccc
       61 ccgatcaggt agaccgtgtt cgggtggccc acctcctgat cgaagaaatg ggggtgaacc
      121 tggagggcgc cgaggtcgct cttcacatgc gcgagcagat gatcgcgatg cagcgtcagt
      181 tcaacgaatt gttgcggttg ctcgggcagc agtttaagaa ataggcaagt gacttttgtg
      241 ttttctcgca cccctgccac atgaaaatat acggaaggcg ggcatttacc cgccttccgt
      301 aacgaccaac ggattgccgt cagcggttat ttacagtgct gttttccccg gaagaaccag
      361 aagagaaatg gtactgtagt tctgaacgca acctgctgac cttcagttaa tacttacgcg
      421 ttcctgttgg gggatcgtcg cctggtttcg tcatcttcga atcggaaccc gctccgcttc
      481 gacctgacgt gccaccggtc gaggatcctc ccgattttga actgattctc acgcccaggc
      541 ggtcggccgt tttttgatcg accataccag tcactgaaaa gccattatct ttttgaaacg
      601 cccgaatagc ctcacgcgcc tcatcgtccg ccactccgtc gattttacca ggcttatatc
      661 cctttgtttg taaggcctcc tcgactgctc tcatatcatt ctttgacaat tcgggagtgc
      721 ccaactggtt aacccctggc acgtcttgat cgcggccagg tcgagtttga ttgggagccg
      781 attgtcgtct ttccccggga agctcttgag accagagcgg actcgatgca aatccgaccg
      841 ccccactcag aattattgct gctactactg gtttttttct tttcatttgt tctcctttct
      901 tgaaaaaaat ttcagttgcg ttcttatgag aaacaaacgc aagtgccatg ccagcgtctt
      961 ttttcgtgga aaaaccaaca caaaaaagat taatcagtac gtgcagtaag gggaatccct
     1021 tcactcgttg ttttttgtct cacatgtgca ctccgccaaa aaaaaccggt gtctattttt
     1081 ttcatacgac tatcgtacgg agtttttgac aagccatgaa ggttgccaag acgatgggtt
     1141 ctggctaagc ggagactggc gacagcgacg ccttgagcag cttttgtcgg actgtctgcg
     1201 atatgttgct tcgtacggat tggtatcggt gatgcgggtt gccgccggcg ccttagtttc
     1261 ctttacttcg gtatagacca tcgatgtagc cgctgtcatc aagttccttg atgaatcggg
     1321 cgtcgacgaa atcttgcggc ttggcggttt tcgctttagg attttcttcc gctaaaaggt
     1381 ccagggccat ttgcaggccg gctgcggaag ggtactgctt gcgagggaac ttgttgtcgg
     1441 gcacggcgcc gtcgtaggtc ttgacgagaa tatcgcgatc cttgatctgc cccaggtatt
     1501 tggcgagcac cttgattcca gtttcccggt cggttttcat caaatgaatc gcttctacgt
     1561 aggacttgac gtagcggcgt actgtttccg ggttgtcacg gatgaagctg cgggttgtcg
     1621 ccaaaccgat atgaacaaaa ggcaagccta attcggccac gtccgccaga acattgaacc
     1681 cctcgcgctg cgcgacaacg ttttgcggcg gactgaaaag cgtcgcttga atttttccca
     1741 ggcgcaggct ggcgatcctt tcggtgactc cgcccacaac aatcagcgag acgtccttgg
     1801 gtgcgaggcc aagcttttgc agcgcgaagc gggtggcggt tgggttcgcg cctctgaacg
     1861 ccgacaggcc gacggtggcg cctttgagct gctcggcggt cttgatctcg ggcctgctca
     1921 ggagcacaaa gtccagtgtg gacatgccgc aggcaacgta cacggcattg gatcctcgca
     1981 gcgcactttc caccaccacc ggtcccgagg cttggacaaa ctgaagctct ccggacatca
     2041 aagccatgac gccgacggaa gcgtggacgc gcacagcctg aacgtccagt ccgttgcgag
     2101 agaagaggcc ggactccttg gcgatccaga gcggcaacac accagccgaa tcggaaacat
     2161 aggaggtcgt caccttggcg agttgcgcgg gtgcctcgca cgggagcgca acgatgtaga
     2221 aaagcgcgaa agagacgagc agccggtcaa aagttttcac cactagaatc tccaatgcgc
     2281 ttcgaaacta actgaaatcg tagggaagcg tcaaggcgat tggaagtatg ggtgaagcag
     2341 gcaacgcgat acgcgccatt gttgctcctt gcggctatga tcgacaacag agtgtttgtc
     2401 ctttcgagac gctgcgggct tgctgatcga gtcgcgtgtg ttgctttttt gtcctgcctg
     2461 aactaatgag gcctatcatg aaggaggacc actcacatgg ccgaaacgaa cattgccgaa
     2521 acggtggtag aatatcttag ttcgaagaac gtccggcata tcttcggtgt cctggcccat
     2581 acttcttttg cactgggcga tgccatagcg aagcggccgc acattaggtt cgtcaattcg
     2641 cagcacgagg gcggcgcagg aaacatggcc ctggggtacg cccgcgccgc caagaagccg
     2701 gcggtttgcc tggtttccgc gggcggcggc gccaccaaca tcgtcacagc tgtcgcacaa
     2761 gcttataaag aagctgtgcc gctgttcgtc atctcttcgg acatcaacac ggcttcttca
     2821 agcaaaggcg cttggtcttc ctggcatggc atggagcacg tgaggctttt tgagccgatc
     2881 accaagcaga gtttgaagct cgagagagtc gaagagatcg gctccgtctt ggattcgctg
     2941 tttcgcttaa ccacgcgtgg acggcaaggt ccggtttttc tcatcattcc ggctgatctg
     3001 caggcggtag cccttcccga accgctgatg ttctccccga cggcggctcc cgccgctcct
     3061 tccatcgatt cgcgctcaac cgaaatggcg gtcgatctgc tgctgaaggc aactgcgccc
     3121 gtcgtacttg tgggcaccgg tgtcgattgg tccggcgcgc aaagagaagt tcaagaacta
     3181 gcggagctct tgtcgctgcc ggtagctacc agctataccg ccaaaggcgt tttccccgag
     3241 aatcatccgc tggcgctcgg ctgtctcgga gtcggtggga ggccgtacgc aaaagagttg
     3301 ttcctcaagt ccgatctgat tttggccctg ggcacaactt tcagcgaagg gacgacgatg
     3361 ggtttcgggc atcgaacgat tcccgatcag gcgaaaatta tccagatcga catcgacccc
     3421 gacgagctcg ggcggaatta tccgactcaa ttggccatgc aagcggatgt gaaagcggcg
     3481 ctgggcgcga ttatcgacgg catcaaaagc aaacggccga gcgcaccggc tgccggtggt
     3541 ggcggaagag ctcgagagat agagcgggcg aagcaggcgt ggcggagcga tctcgataaa
     3601 aaaactgcca acggaaacct aacctacgac tcggtgctca aaactctcaa tgaactgttg
     3661 accggaacag agatattggt gacttccggc atgaccggcg acctgctgcg aaacatcgac
     3721 gccgcgacgc cgatcattca cgccggtgag tttcgcgcca tcggcaccgc gtttgcgact
     3781 tccctcggtg tcaagctcgg ccttccggac gcgcgggtcg tctgcgtcac cggcgatggt
     3841 tcattcatga tggaacagca agagctcgcg acggcccggt tgcataacat tccagtgctg
     3901 gccatcgtcc tgcgcaataa cgcgtacggt gggatgaaac gcgatcaact ccatcattac
     3961 ggcgggcggg tgatcggcac cgagcttttc gtgccggacc tcgcgcgcat ggccgagctg
     4021 ttcggtgcta agggatacac gatcactcga cccgaggagg tgaggccggt tctacagaaa
     4081 tccctcgagt ccgatgattt cgttgtggtc gatgtaaagg tggataaatg aaagcaacgc
     4141 cgggtcaaaa tcaccggtca actcgcaaca gatcggtcgc tattttttag agcagcacgt
     4201 gcataaaaat taacaccttt attccgaaag gaagacagga aaggagtcaa gccatggtaa
     4261 tcttcagtat gaagaagcta atggtcacgg tgctcgcagc tcttcacctg ctgtttgtcg
     4321 ccgccctcac catagaaagc gtgcaagcag ccgaagcaaa gcaggcagcg gcgaagcgtc
     4381 acaagcttcc cgccactttg gaaacaacgc agtggggatg gcttgatcct aacgagtctc
     4441 ccaagcttac ggtggattca ggggatatcg tctcgattga gacgatgatg cattcccata
     4501 acaagatcca gcctggcact acgatggatg agatcgtcgc cctgaggaag gccaatcccg
     4561 gtgggggacc tcactctgta acgggtccca tttatgtcaa tggtgccgag cccggagatg
     4621 tcatggagat tcgcattcta agaattgtcc ctaaaccatt tggagttaac tttaacctgc
     4681 cgggcaaaga attcccgacc atcggtgccc tggccaccga atttccggaa ggtttcgtca
     4741 agtacttcaa ttttgattcc aagaaccggc aagccgagtt taaacccggc gtcatgatcg
     4801 atctgcagcc gtttcccggc acactcgcag tcggcatcga tccggacgac cctgcaccgc
     4861 gtaaaggtgg agtaaaagat tccatggcgc cggtctctac tcttcgacct tggaagaacg
     4921 gatctaatat ggatctgaac gaactccagg aaggaagcat catctacatt ccgatctttc
     4981 tcaagggcgg gctcgtatgg acgggagact cgcactgccg acagggaaac ggtgaggtca
     5041 acctcacggc tctcgagtgt tcgttccgag agattgcgct ccagttgatc gtgcgcaaag
     5101 acatgaaact cgaatggccg cgagccgaaa ccaaaactca ttggattttg atgggcttcg
     5161 atgaagacct gaataaagcg atggttagcg cggtccgcga gacggtggac ttcctggcaa
     5221 atcaaaaaat ggtgccattg gatcgctacg aggcctattc catcacctcg atgacggctg
     5281 actgccgtgt gacccaggtg gtcgatgtcc gcaaaggggt ccattgcatg gtgccaaagt
     5341 ctatttttac gaagaagaag tagcgggtgc tttgcgcgct tgcattcttc ttgctgtgga
     5401 ccgccgccgc ttggggtgcc gagccgcttc ctgtagtcac tacctcgacg gacctcaaag
     5461 ccctggttga agttgtgggc ggggaaaggg ttcgggtaga gagcctagct tctcctctcc
     5521 aggatccgca cgctgtggag gtgaagcctg gccaactaac caagctcaaa gtggccgctt
     5581 tgctcgtaag gattggcttg gaccatgagc catggcttgg ccgcgcgctc cgcacggtgg
     5641 gcgactcgcg cttcacgcgg gagagcccgc actatctcga tacctccaaa ggtatacaac
     5701 tgctccaggc cgagactccg agagtgaggg cggataaggg cgctcacctt cacgggttcg
     5761 gcaataccca ctactggctc gacccggaaa atggccggcc tatcactgca gctatcctga
     5821 cagccctgct ccgactagct cctgcagacc gtccctattt tgaggccaat agaaaacgat
     5881 ttctcgagca attagatgac cggctaaaag gttggtcaca gacaatggct ccgtacaaag
     5941 gaacgcgact ggtcgcggtt catgagactt ggccctattt cgcccaacgc ttcggtctga
     6001 caattgtcgc tg
//
LOCUS       DASSWZ010000347         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_353771, whole genome shotgun sequence.
ACCESSION   DASSWZ010000347 DASSWZ010000000
VERSION     DASSWZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353771"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VFN58_08335"
     CDS             complement(<1..1272)
                     /locus_tag="VFN58_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HET8918035.1"
                     /translation="METAAREFQIMVKTVGAICNLDCHYCYYLKKEELYPKGSNFRLN
                     DETLERYIIQHIQATPKEMVSFSWHGGEPTLLGVDFFRKAVELQKKHLPEGKKIINGI
                     QTNGTTVNEEWCEFLAKENFYIGLSLDGPKDLHDHYRVTKGQKPTHKQVVQAFQMLRR
                     AKVHVDLLCVVHDVNVRHPTQVYRYFKEIGGQYLQFLPLVEINDDAARSINPRSVPAA
                     AYGEFLCTIFDEWVRNDMGKIFIQLFDESVRPFLGMEHALCIYRETCGDVPVIEHNGD
                     FYSCDHYVLPEYKIGNIYEQTLAEMVEDPRQRDFGQKKWTSLPRYCRECEVLSMCNGG
                     CPKDRVIKTPDGEAGLNYLCAGLKQFFTHSKPYFRKFAELADAGEPTEKLMEIVRAAD
                     AKTFVRQAGRNDPCPCGSGKKYKRCCMGKRTY"
     gene            1441..1755
                     /locus_tag="VFN58_08340"
     CDS             1441..1755
                     /locus_tag="VFN58_08340"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET8918036.1"
                     /translation="MIDFGKITGFEWDEGNRDKNWDNHKVAWWEIEETFFHQPFVLAP
                     DPSHSDQETRFYALGHTKGGRLLQIVFTIRGTKIRPISARNMSRKERKVYGQAAKEGA
                     DL"
     gene            1721..2020
                     /locus_tag="VFN58_08345"
     CDS             1721..2020
                     /locus_tag="VFN58_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HET8918037.1"
                     /translation="MAKQRKKVPIFKSEDEEREFWARHDSTDYIDYKKAKRVQFPNLK
                     PSSRAISIRLPESLIENIKVLANREDVPYQSMMKVLLAEKVTEALRSPKRKKTAA"
BASE COUNT          524 a          568 c          578 g          545 t
ORIGIN      
        1 gtaagttcgc ttccccatgc agcacctttt gtacttcttc ccgctcccgc atgggcaggg
       61 atcattgcgg ccggcttgac ggacaaacgt cttggcgtct gctgcgcgca cgatctccat
      121 gagtttttcc gttggctccc cggcgtccgc caactcggcg aactttctaa aatagggttt
      181 gctatgcgtg aaaaactgtt ttagccccgc acacaggtaa ttcagtccgg cttcaccatc
      241 aggggtcttg atgactcgat ccttgggaca gccgccattg cacatgctca agacttcgca
      301 ctcgcggcag tagcgcggca agctcgtcca cttcttttgt ccgaagtcac gctgccgtgg
      361 gtcctcaacc atttccgcaa gggtctgctc gtagatattg ccgatcttgt attcaggcaa
      421 aacgtagtga tcgcaagaat aaaaatcgcc gttgtgctcg atgaccggga catcgccaca
      481 ggtttcacgg tagatgcaaa gggcatgttc cattcctaaa aacgggcgca cggactcgtc
      541 aaagagctgg atgaaaatct ttcccatgtc gtttcgtacc cactcgtcga agatcgtgca
      601 aagaaactca ccgtaggccg ccgcggggac tgagcgggga ttgatcgacc tcgcggcatc
      661 gtcgttgatt tcgacgaggg ggagaaactg caggtactgc ccgccgatct ctttgaagta
      721 gcgatacact tgggtcggat gcctgacgtt aacgtcgtga acaacacaga ggagatcaac
      781 gtgcactttg gcccgccgga gcatctggaa agcctgcacg acttgtttgt gagtgggttt
      841 ttgccccttg gtgacgcggt agtgatcgtg gaggtccttt ggcccgtcta gactgaggcc
      901 gatataaaaa ttctccttgg cgagaaattc gcaccactcc tcattgaccg tcgtgccatt
      961 agtctgaata ccgttgatga tctttttgcc ctcgggtaga tgcttctttt gtagctcgac
     1021 agccttgcgg aaaaagtcga cgccgagcaa ggtcggctcg ccgccgtgcc aggagaagct
     1081 taccatctcc ttgggcgtcg cttggatatg ctggatgata tagcgctcta aagtttcgtc
     1141 gtttagacga aaattcgatc ctttgggata gagctcttcc tttttgaggt aatagcagta
     1201 gtgacagtcg aggttacaga tcgcacccac tgtcttcacc atgatctgaa attcacgcgc
     1261 ggcagtttcc aaagggatta cctccgagct cagggcttga ggcactctac tcaaaaaagc
     1321 cgtcgatgtc atcacctttg gaggccactt tagaatgtgg ttcggtgggg ccttttacgc
     1381 tgcttgacgt ctcactccga gtgtatatca tatctatata caaccgagtt acggctttta
     1441 atgattgatt tcggcaagat cacggggttt gagtgggacg aaggaaatcg ggacaagaat
     1501 tgggacaatc acaaggtcgc gtggtgggag attgaggaga cattcttcca tcagccattc
     1561 gttcttgcac ccgacccctc tcattccgac caagaaactc ggttttatgc ccttggtcat
     1621 accaagggtg gccgactact gcagattgta tttactatcc ggggcacaaa aattcggccg
     1681 atctctgccc ggaatatgtc cagaaaggag cggaaggtct atggccaagc agcgaaagaa
     1741 ggtgccgatc tttaaatccg aggacgaaga acgggagttt tgggcacgcc acgactccac
     1801 cgactatatc gactataaga aggcgaaacg cgtccagttt ccgaatctga agccttcgag
     1861 tcgggcgatc tccatccgtt taccggagtc cttgatcgag aacatcaagg tcctcgcaaa
     1921 ccgggaagac gttccctatc agtcgatgat gaaagttctc ttggcggaaa aggtgactga
     1981 agcacttcgt tccccgaagc gtaaaaagac cgcggcctaa taaggtggtc aacctaacgg
     2041 tcagtgatat ccggtagcga ttcttctgcc tgacaatgag atggaaaatt tggttctagc
     2101 gaacccaatc agctatgaaa atattgaact cccttggctc tttagcgttc ctcgtggaag
     2161 cgaagaccaa tttcttcccg tcccgtgaca acatcggaaa gctggaaaag aaaaa
//
LOCUS       DASSWZ010000348         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U637
            k141_1131944, whole genome shotgun sequence.
ACCESSION   DASSWZ010000348 DASSWZ010000000
VERSION     DASSWZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968660
            Sequence Read Archive: SRR25158317
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 08:00:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,674
            CDSs (total)                      :: 1,657
            Genes (coding)                    :: 1,646
            CDSs (with protein)               :: 1,646
            Genes (RNA)                       :: 17
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 4 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 0 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131944"
                     /isolate="SMAG_U637"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.32083333 N 106.1394444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFN58_08350"
     CDS             complement(<1..849)
                     /locus_tag="VFN58_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET8918038.1"
                     /translation="MLVLSEGQVRSLIDVEELITALGQAHIQYSTGKAVMPVRLVVPL
                     PQIHGRITSMPGYLTEDKALGMKVVTYFQNNPEQNLPAILATVMLFSAATGKIIAVMD
                     GSYITAIRTACASAMATRALANPATPVLGILGAGVQARAHIQALCQVRKLNQIKLYSP
                     SGASAASVKRELEPAVGVAIDVVKSAEETVRNSDLVVTATTAEQPILKCEWLKPGTHI
                     NAVGSHRPDSREVDGPTVAQSKVVVDSREAIMAECGDILLAIKEKTIAENHVHAEIGE
                     VLAGIKP"
     gene            921..1460
                     /locus_tag="VFN58_08355"
     CDS             921..1460
                     /locus_tag="VFN58_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET8918039.1"
                     /translation="MARHKHVINLCEVAVDRINAPEDSPFGGLRQRVGAHIGAQKLGY
                     SFFNVPPGKAAFPYHTHSGNEEMIYIIDGAGVLRFGKEELAVTTGTVIACPPGAEYPH
                     QLINTGDKELRYLAVSTMEFPDLCEYPDSNKVGAYATAAVGQQVGFRALYVKDKNVNY
                     YEGEDGKEIDRVKRSLAKA"
     gene            complement(1476..2132)
                     /locus_tag="VFN58_08360"
     CDS             complement(1476..2132)
                     /locus_tag="VFN58_08360"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET8918040.1"
                     /translation="MSKTETANSKAQNEALENSSAAVRVLKGGGVIVFPTETLYGLGA
                     DALNNAAVEKVFQLKGRDPRSPIPVLVADQEMLHTLVAQVPTTAQKLIDRYWPGPLTL
                     VLPGQKNIPKPLCNPAGGVGVRISSQPIATLLVNELGRPLTATSANPSGKEPARTLQE
                     AKTYFAGRVELFVDGGALTSKSGSTVVEVMEDSIKIIREGELSASELRQVLGEEKVVD
                     "
     gene            complement(2083..2607)
                     /gene="purE"
                     /locus_tag="VFN58_08365"
     CDS             complement(2083..2607)
                     /gene="purE"
                     /locus_tag="VFN58_08365"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET8918041.1"
                     /translation="MAKNSTGKPRVGILMGSDSDLEVMREAEKRLDYFGITYETRIMS
                     AHRTPDKAAKYAATAQQRGLEVIICGAGAAAHLAGAIAANTNLPVIGIPIDSSSLQGL
                     DALLATVQMPAGIPVATMAIGKAGAANAGIFAAQIVARKDSKVAAKLGEFKKEMAAGV
                     EDRDRKLQSAKRSA"
     gene            complement(2625..>3254)
                     /locus_tag="VFN58_08370"
     CDS             complement(2625..>3254)
                     /locus_tag="VFN58_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HET8918042.1"
                     /translation="VFDNDQGPNTGGMGAYSPAPVVTPAVQKRIFDEILNPLLTGLKK
                     KGITYRGVIYVGLMITKTGPKVLEFNVRFGDPECQPIMMRLKSDLVPLLEATIDGKLN
                     QVEAEWYEDPAVCVVLSARGYPGPYDKGCEIRGLDKLRHWEKGFVFHAGTTKENGRWL
                     TSGGRVLGVTARGSDIGNAVTNAYAAVSQISWDGMHYRKDIARRAILRN"
BASE COUNT          708 a          912 c          855 g          779 t
ORIGIN      
        1 cggttttatc ccagccagca cttcgccgat ttcggcgtgg acatggttct cagcgatggt
       61 tttctctttg atggccaaga gaatatcgcc acactcggcc atgatcgcct cacgtgaatc
      121 gacgactact tttgactgcg ctacggtcgg gccgtcgact tcccgagagt ctggacgatg
      181 ggacccgacc gcattgatat gcgtgccggg cttcaaccat tcgcatttta aaattggttg
      241 ctccgccgtt gtggcagtaa ccaccaaatc ggaattccgc acggtttcct cagcgctctt
      301 gaccacatcg atggcaactc ccacggcggg ctccagctct cgcttgacac ttgcggcgct
      361 cgcgccagaa ggactgtata gtttgatctg gttcaatttt ctgacctgac acaaagcctg
      421 aatgtgtgct cgggcttgaa caccggcgcc cagtattccc aacaccggcg ttgctgggtt
      481 tgctaacgcc ctggtcgcca tagccgaagc gcaagccgtg cggatggcgg taatatagct
      541 tccgtccatg acggcaatta ttttacccgt agccgcgctg aatagcatta ccgtcgcgag
      601 aattgccggc agattttgct cagggttatt ctgaaaatag gtcacgacct tcatgcccag
      661 ggctttgtcc tcagttagat atcccggcat gctggtgatt cgcccgtgga tttgtggcag
      721 cggcaccaca agccgcacgg gcatgaccgc tttccccgtg gaatattgga tatgagcctg
      781 acccagcgcg gtgatcagct cctcaacatc gatgaggctt cgaacctgcc cttcagataa
      841 gacgagcata aatgattctg atagcacaaa gacagcggcg aatggtagtg tgtgaaaacg
      901 aaagctcacg aggaggcgtt atggcgagac acaaacatgt gatcaatctt tgtgaagtgg
      961 cagtcgatag gatcaacgcg ccggaagact ccccctttgg gggtttaaga caacgcgtgg
     1021 gcgcgcacat aggcgcgcaa aaacttggct acagcttctt caacgttcct cccggcaagg
     1081 cggcgtttcc gtaccatacg cacagcggca atgaagaaat gatttacatt atcgacggag
     1141 caggggtgct caggtttgga aaggaagaac ttgcggtcac cacgggaaca gtcatcgcgt
     1201 gtccgccagg agcggaatat cctcaccagc ttatcaacac gggggacaag gaacttcgtt
     1261 acctggctgt cagtacgatg gagttccccg atctctgcga gtaccctgac tctaataaag
     1321 ttggcgctta tgcgaccgct gcggttggcc agcaggttgg atttcgcgcg ctttatgtta
     1381 aagacaaaaa cgtcaattac tatgaagggg aggatggcaa agagatcgac cgggtgaaga
     1441 ggtcccttgc aaaagcatga cgacggactg agttttcagt ctacgacttt ttcctcgccg
     1501 agaacttgtc gaagttcaga tgcactgagt tcgccttcgc ggattatttt tatgctgtct
     1561 tccatgacct ccaccaccgt ggagccgctt tttgatgtta gtgctccacc atctacgaaa
     1621 agttcgaccc ggccggcaaa ataggtcttt gcctcttgaa gagtccgtgc cggttctttg
     1681 ccggaggggt tggcgctggt ggcggtcagc gggcgaccca actcattgac gagtaacgtt
     1741 gcaatcggct ggctcgaaat ccgcacgccg actccccccg cgggattaca aagcggcttc
     1801 ggaatatttt tttgcccagg cagaaccaag gttagcggtc caggccagta tcgatctatc
     1861 aatttctgag ctgtcgtcgg tacctgcgcg accaaggtat gaagcatttc ctggtcggcc
     1921 acgagcacag ggatcggact gcgtggatcc cggcctttta gctgaaatac tttctcgacg
     1981 gctgcattgt taagcgcgtc tgcacccaat ccatagagag tttccgtggg gaaaacgatc
     2041 acaccaccgc ccttgagtac ccggacggcc gcggacgagt tctcaagcgc ttcgttttgc
     2101 gctttggagt ttgcggtctc ggtcttcgac acccgcggcc atctcctttt tgaattcacc
     2161 gagcttggcg gcgactttgg aatctttgcg ggcgacaatc tgagcggcaa agatcccggc
     2221 attggctgcg cctgccttgc cgattgccat ggtagccact ggaattcccg ctggcatttg
     2281 taccgtagct aggagcgcgt ccaaaccttg gagactggat gaatcaatgg gaataccgat
     2341 gaccggtaag ttagtgtttg cggcgatggc ccccgcaaga tgagccgcgg cgcccgcgcc
     2401 gcagataata acttcaagcc cacgttgctg cgcagtcgcg gcgtatttcg ccgccttgtc
     2461 cggtgtgcgg tgcgcggaca taatgcgggt ctcgtaggta attccaaagt agtcgagccg
     2521 cttctctgcc tcccgcatca cttctaaatc agaatcgctg cccatcaaaa taccgactct
     2581 tggcttcccg gttgaattct ttgccacctc tgatttcctc tcggtcaatt tctaagtata
     2641 gctctgcgag caatatcctt gcgatagtgc atcccgtccc aactaatttg actcacggcg
     2701 gcataagcat tcgttacggc gttgccaatg tcgcttcccc tcgcagtgac gccgagcacg
     2761 cggcccccgg aggtaagcca gcgaccgttc tccttcgtag tgcctgcgtg aaagacaaag
     2821 cccttctccc aatgccttaa tttatccaat ccgcgaatct cgcagccttt gtcgtacggt
     2881 cccggataac ctctcgcgga caacaccacg caaacggccg gatcttcgta ccactccgcc
     2941 tcaacctgat tgagtttccc atcgatggtt gcttccaaaa gcggcacgag atcacttttc
     3001 aaccgcatca tgatcggctg gcattcggga tcgccgaaac ggacgttgaa ctccaacacc
     3061 tttggtccag tttttgtaat catcaaacca acatagatca cgccacgata tgtaattccc
     3121 ttcttcttta agccggtgag caatggattg agaatctcat caaaaatccg cttttgtacg
     3181 gcgggtgtta ctacaggcgc gggagaataa gcgcccatgc cccccgtgtt tggaccttgg
     3241 tcgttgtcaa agac
//
